LOCUS       DANLXS010000001        64152 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.001, whole genome shotgun sequence.
ACCESSION   DANLXS010000001 DANLXS010000000
VERSION     DANLXS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 64152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64152
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.001"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            8..442
                     /locus_tag="PXD71_000001"
     CDS             8..442
                     /locus_tag="PXD71_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL6290175.1"
                     /translation="MEQLDITVLENAFNSLEKTIVSLENQAWFNRQEAIIQDTLIAGA
                     IQKFEFVYELSIKMLKRQLKNIESNDTDIDSTEFRDILRMAAQYGLIESPEVWLDYRK
                     MRNITSHTYDQNKAQQVYNGISNFLESTYFLVKQLQKRNSYD"
     gene            435..728
                     /locus_tag="PXD71_000002"
     CDS             435..728
                     /locus_tag="PXD71_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL6290176.1"
                     /translation="MIDLTESQRQQVLDIFKKYLPDTPVWVFGSRIKGTAKAYSDLDL
                     ALITKQPLTIRQQTELELAFTDSDLPFTVDLIDWASCNESFKQIILQRYEVLI"
     gene            777..1811
                     /locus_tag="PXD71_000003"
     CDS             777..1811
                     /locus_tag="PXD71_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL6290177.1"
                     /translation="MKKFFLLLIAFFTLSACEKAPEQITLSGKTMGTTYTVKYIDNGE
                     VENLISPEKMKNELDGLLIEVNNQMSTYQQDSEISRFNQAKQANQAVEISQDFAKVVQ
                     EAIRLNKVTEGALDVTVGPLVNLWGFGPDKRLNKVPSDEQIAERSAYVGIDKILLEMS
                     SKPKLTKSAANLYLDLSSIAKGFGVDKLAEHLETIGVSNYLVEIGGELRGKGKNLQGL
                     DWRIAIEKPVMTQGQAAQITVPLHNLAMATSGNYRNYFEDEKGNRLSHIIDPKQLRPV
                     NHNLASITVFAPTTMTADGLSTGLFVLGAEKALAVAEREKLAIFLIVKNGDKFETQMS
                     SEFSKLIQSQ"
     gene            1824..2069
                     /gene="nqrM"
                     /locus_tag="PXD71_000004"
     CDS             1824..2069
                     /gene="nqrM"
                     /locus_tag="PXD71_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HDL6290178.1"
                     /translation="METVLLTFGFFVAVIFAMSIGFIVKGKTIKGSCGGITALGMKKM
                     CDCEEPCDNLKSKIADGTADPEEVARFNKEPQFYEVK"
     gene            2071..2487
                     /locus_tag="PXD71_000005"
     CDS             2071..2487
                     /locus_tag="PXD71_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL6290179.1"
                     /translation="MNRDIRWKQRFDNYKKSVSYLQAEAEKYADTDIDVIKKGIIQSF
                     EITHELAWKLMQDILKWEGEVDIYGSKTASRLAFNRGLITQGEVWLEMIKSRNITVHT
                     DDETILTEEFNKIIKAYLPLFIEFEQRVETLCQTLD"
     gene            2469..2780
                     /locus_tag="PXD71_000006"
     CDS             2469..2780
                     /locus_tag="PXD71_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL6290180.1"
                     /translation="MPNFGLSERATKTITDILAKYPEVEQAIIYGSRAKGNYREGSDV
                     DLTFKGKDLTYDIVKRIWGDLDDSDSPYLFDLSIYHQLTNANFIEHIDRVGKVFYQRE
                     L"
     gene            2891..4054
                     /gene="mnmA"
                     /locus_tag="PXD71_000007"
     CDS             2891..4054
                     /gene="mnmA"
                     /locus_tag="PXD71_000007"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL6290181.1"
                     /translation="MTTQLTSKTYDTHFPKLTPEQLAENSKKKVIIGMSGGVDSSVSA
                     FILQQQGYQVEGLFMKNWEEDDDTDYCTAAAELADAQAVADKLGMKLHKINFAAEYWD
                     NVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEYAAEDLGADYIATGHYVRRTSDDQD
                     AKLLRGLDENKDQSYFLYALNKKQIGQSLFPVGEIEKPIVRAIAEDLGLATAKKKDST
                     GICFIGERKFKDFLARFLPAQPGEIRTVEGKLVGRHDGLMYHTLGQRKGLGIGGVKGL
                     SEDPFYVVEKDLINNVLIVAQGHDNSALLSSGLIAKQLTWVDMQPLRENLRCTVKTRY
                     RQADIACEIQVIDDETIRVMFDEPQIAVTPGQSAVFYLGEVCLGGGIIESQLK"
     gene            4171..5550
                     /gene="argH"
                     /locus_tag="PXD71_000008"
     CDS             4171..5550
                     /gene="argH"
                     /locus_tag="PXD71_000008"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDL6290182.1"
                     /translation="MALWGGRFTQQADAKFKYFNDSLRFDYRLAIQDIEGSIGWAKAI
                     TSVGILTVAELEQLIAALKEVRAEVESNLTIILKDDAEDIHSWVESKLIEKVGDLGKK
                     LHTGRSRNDQVAVDIKMWCKVQAVALQERIRALQERLVSVAEENQESVMPGYTHLQRA
                     QPVTFAHWCMAYYEMLERDFSRLSDANKRMSTCPLGSGALAGTAYGIDRDLLARDLGF
                     EEATRNSLDSVSDRDHILELLSTASISMMHLSRFAEDLIIFNSGESGFLEMSDRVTSG
                     SSLMPQKKNPDACELIRGKSGRVFGALNGLLTTLKGLPLAYNKDMQEDKEGIFDALET
                     WQACLEIAELVLVDIQVNTERTREAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VYAISKKEPLEALSINEFQQFHHSIGDDVYPILSLESCLEKRCAKGGVNPQRVAEAIA
                     VAKANLASK"
     gene            5718..6716
                     /locus_tag="PXD71_000009"
     CDS             5718..6716
                     /locus_tag="PXD71_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HDL6290183.1"
                     /translation="MTESVNSTQAGTALSLGQQLKAAREALNLSITDVAQKTNLKKSH
                     IESLENDIFILQNVAPTFVRGYVRNYVKFLRLPESLISSVNYGEVTIPKEVTKVSSVK
                     PSSNSQGKTLKYLTVLVLLAALGMTLLWWWQGYQKEQENREQFVSSAPVAEVANTATN
                     NDASAQTSVPVQQPIQVSEQPAQVAETVVAAEPALTEKVEPTQAASSPVTQEDVKVVE
                     EVKQADATTAQVEAVNVLQQSQSEQAPTEQPVAVTNDELRIEITGSQSWITVRGAKNK
                     RLAEKLYNNGETLSFNENEQYRLTIGAPANVKVYYKGQEVPLKIDGRVARIHLPLQ"
     gene            6817..7929
                     /gene="ispG"
                     /locus_tag="PXD71_000010"
                     /gene_synonym="gcpE"
     CDS             6817..7929
                     /gene="ispG"
                     /locus_tag="PXD71_000010"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDL6290184.1"
                     /translation="MIHQSPIKRRVSKKIYVGNVPVGGDAPITVQSMTNTRTTDVEAT
                     VAQIKSLERVGADIVRVSVPTMDAAEAFKAIKQQVSVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRTVVDCARDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLES
                     ALRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARAGSV
                     KSAVGLGLLLAEGIGDTLRVSLAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EIDVIATVNELEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGVTGSNKMSGFYLDGV
                     RQKERFDNAKLIDQLEAKIRARVAQQHNRIDIEQIG"
     gene            7935..9206
                     /gene="hisS"
                     /locus_tag="PXD71_000011"
     CDS             7935..9206
                     /gene="hisS"
                     /locus_tag="PXD71_000011"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL6290185.1"
                     /translation="MAKTIQAIRGMNDCSPSESPLWQWVEQKVRNVLASYGYSEVRMP
                     IVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQ
                     RLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIANPEIDAELIILTARLWKELGIDQHV
                     SLQLNSIGSLEARANYRSALVKFLENHTALMNEEEKERLVKNPLRILDTKNEALQNVL
                     NDAPKLLDYLDDDSREHFTQLCALLDAMGIRYEVNPKLVRGLDYYNKTVFEWVTSALG
                     SQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEIDLPKTVDVYIVF
                     SGEGATLNAFQLAEQIRSELPHVGVMTHCSGGKFQKQFKRADKVEAQIALVIGESEVA
                     EKQVVIKDLRSGAEQITIAQSELIAELSKRF"
     gene            9216..9836
                     /locus_tag="PXD71_000012"
     CDS             9216..9836
                     /locus_tag="PXD71_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL6290186.1"
                     /translation="MSNEYLNKTEEQQFEDAKNWFKENGTPILVAIILACAATAGWNF
                     WKNHQLETAQKTSASYQSVMESYLQNPSKNQPLVEKFIADNKDSSYAAFAQFEVAKQA
                     VEKGNFSAAKTALQSVLTYSDSTLQSIARFRLAQIDFQLKSYDEALASLAQIQDKAWE
                     LRKQILTGDILAAKGDIPAAKSAYEQAKTTANPQEQTLIDVRLNNL"
     gene            complement(9899..11002)
                     /gene="dnaN"
                     /locus_tag="PXD71_000013"
     CDS             complement(9899..11002)
                     /gene="dnaN"
                     /locus_tag="PXD71_000013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL6290187.1"
                     /translation="MQITITREQLLKPLQQVCGVLSSRPTLPVINNLLLEIEGNRLSL
                     TGTDLEVELTTQAQLLQAVDFAGKFTIPAKKFLDISKSLPDDSVISVQFEEDKAIIKA
                     GRSKFSLATLPASDYPNLMDWKPEVDFSIDQSTLARLIDATQFSMANQDARYFLNGMK
                     FETEGNLLRTVATDGHRLAVCTMVLNQELLTHSVIVPRKAVLELSRLVASNDETVRLE
                     IGSSNIRITMNGIVFTSKLIDGRFPDYRRVLPRNADRILEAETETLKRALMRAAILSN
                     EKFRGVRLTLSNNLLKITANNPEQEEAEEIIDVAYDSPEMEVGFNVSYVLDVLNTLKC
                     QRVRFSLVDSSSSCLIEDCDNSSAEYVIMPMRL"
     gene            complement(11021..12370)
                     /gene="dnaA"
                     /locus_tag="PXD71_000014"
     CDS             complement(11021..12370)
                     /gene="dnaA"
                     /locus_tag="PXD71_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL6290188.1"
                     /translation="MEQIVSSLWSDCLNHLQTKVSPTDYSTWLRPLQASSENGELTLY
                     AQNQFVENWVKDKFLSEITELARFLAKNDNLVVTLKVGIKPTEQPKVSPNLKNESSEF
                     SNTGFKTGLNPNQTFDNFVQGRSNQLAKLVAQQVVDNLGESHCNPLSFYAGTGLGKTH
                     LLHAIGNELVKRTPGTRVLYIHLERFYRDIIKAINSSNNDAEKIKKFYRSLDVLMIDD
                     IQFLANKPKVQEEFLHLLNALFEQRKQIILTSDVLPKNIDKIDTAIKSRLSGGISATI
                     EPPELETRVAILRQKALERGADLQEDAAYLMGQKLRTNVRELEGALNRVIAWQKLTLR
                     PITVDAVRETLQDLFASFDYLITIENIQRIVAEYYGIKVADIKSKSRARSIARPRQMA
                     MALAKELTNHSLPEIGREFGGRDHTTVMHACKTINELRDTESGIQEDYIHLTRKLSS"
     gene            complement(12557..14404)
                     /locus_tag="PXD71_000015"
     CDS             complement(12557..14404)
                     /locus_tag="PXD71_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6290189.1"
                     /translation="MFEKLFKWFEARVETYPNEMPKTPKSGLIPFIYDATKGMRFYIL
                     LLTVLVAAVGIIEAVLFRFMGDLVDWVNKYSPTELWAEKGWTIIGMFFIALLAVVFVY
                     LASSIRFQSLQGVFPMRLRWNFHRLMLGQSLGFYQDEFAGRVSAKVMQTALSVRDVIM
                     TCADMLVYVLVYFTTSSVILLQLDGWLFVPFILWVISLAATIIYFVPKLAQAAQEQSD
                     ARSLMTGRITDAYANIATVKLFSHGNRESAYAKESMEGFMVTVHKQMRLVTVIETVTN
                     LTSMALIISTAGIGLWLWGSSVVTAGAIATSTALALRIKGLSQWIMWEFARLFENLGT
                     VQDGMITLSKPHNVVDKIDAKPLNVTKGEIKFENVDFAYDPKKPLLKDFDLTIKPGEK
                     VGLVGRSGAGKSTLTNLLLRFYDIQDGSITIDGQNIRDITQESLRSQIGLVTQDTSLL
                     HRSVRENLMYGRPNATEEEMIKAVEQAAASEFIPNLQDAKGRTGFDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEVAIQENLTQLMEGKTVVAIAHRLST
                     IMAMDRLIVLDKGEIVEQGTHEELLAQNGVYARLWAHQSGGFLPENTDI"
     gene            complement(14473..14982)
                     /gene="luxS"
                     /locus_tag="PXD71_000016"
     CDS             complement(14473..14982)
                     /gene="luxS"
                     /locus_tag="PXD71_000016"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL6290190.1"
                     /translation="MPLLDSFKVDHTKMNAPAVRVAKTMTTPKGDTITVFDLRFCRPN
                     IDILPVRGIHTMEHLFAGFMRDHLNSEQVEIIDISPMGCRTGFYMSLIGSPSAEEVVK
                     AWTASMEDALHKIPDVSAIPELNEYQCGSYKEHSLKEAHQIARNVLAAGIGINRNEDL
                     ALDEKLLNP"
     gene            complement(15000..17666)
                     /locus_tag="PXD71_000017"
     CDS             complement(15000..17666)
                     /locus_tag="PXD71_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HDL6290191.1"
                     /translation="MTMEENTHTVVDAQIRQPRKISPFWLLPIVAFIIGALLFFQILK
                     EQGETITIRFSKGDGITAGKTAIRYQGLQIGQVKRVYFVDNLKEVEVQAEINPEAKSI
                     LRKQTKFWLVQPSASLAGVSGLDAIVSGNYITLLPGEGDFVDEFIAEEDPPAITVSDG
                     DLLIRLISDDIGSITVGASVYFRKVPVGNIADYRFTKDQKKIEIDVVINQKYAHLVKK
                     ESRFWNISGISADVNLQGIKINMDSLASVVQGAVAFDSPDNMEIAEQGQKFELYPDLK
                     SAKRGIEINVKLPLTHNLKINETGVFHQNVQIGMLSQLKLPQVKDNGVPQQESGQHKQ
                     IDGVLLIDPSYENLLRSQTKILLKEPKFSLNTEQLTKVGELLRGVYFEVHPGEGEPQS
                     EFVVQNEADYLLSLPNLLSFSLIAPQAYGVDTNQGIYYSDVQIGEIVKRSLELDKVRF
                     DVIIYPPYRNLIGSNSKFVAISNIDMSVGLDGLRVHAGSPSEWLKGGVRLINGKNDGS
                     PKKQYALYKDIESAESGMATLDKKATTTLSADSLSGISEGSLVLYRDFQVGEVLKITP
                     KQQKFEVELFIQPNYRHLLTEKSRFWIEPATSVDVSLKGVNITTAPLMRTLKGAISFD
                     DGGSKGNKTLYASKEKATSGNTYLTLIAKDASKLSEGMDIKYMGLTVGQIEKLELQNA
                     KKQIKATAYIQRKYYPLIAKAGSKFNVISPEISTSGFKNLDAALQNYITVDVGSGQAQ
                     NQFVLADTDTVKTTYANGFPIIVETTNANGIQPEAPVLYRGMQVGIVSRLGLSELGDR
                     VLIYVNIQDKYKHLVRTNTQFWQASGYTMDVSLQGVSMNSGTMSQLLNGGIEFSTPYT
                     KVVKPQAQPNRRFFLQRKLPDEAPAWDQGIAE"
     gene            complement(17749..18189)
                     /gene="fur"
                     /locus_tag="PXD71_000018"
     CDS             complement(17749..18189)
                     /gene="fur"
                     /locus_tag="PXD71_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDL6290192.1"
                     /translation="MSEANIKRLKEVGLKVTEPRLTILALMQDIRDEMQHFAAEDIYK
                     ILLEKGSDIGLATVYRVLNQFEEAGILTRHNFDANKAVFELNMNHEHDHIICMDCGKV
                     FEFKDPDMERRQREISEKHGMELTNHSLYLYGKCSNLTNCDEKK"
     gene            complement(18225..18749)
                     /gene="fldA"
                     /locus_tag="PXD71_000019"
     CDS             complement(18225..18749)
                     /gene="fldA"
                     /locus_tag="PXD71_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDL6290193.1"
                     /translation="MAVVGLFYGSDTGNTENISKMIQKKLGAELIDIRDIAKSTKEDI
                     EAYDFLMFGIPTWYYGESQADWDDFMPTLKEIDFNGKVVAIFGCGDQEDYAEYFCDAM
                     GTVRDVVEPNGGVIVGHWPTAGYTFEVSQALVDDDTFVGLCIDEDRQPELTTERVNKW
                     CEQLKEEMCLDQLA"
     gene            complement(18755..19030)
                     /gene="ybfE"
                     /locus_tag="PXD71_000020"
     CDS             complement(18755..19030)
                     /gene="ybfE"
                     /locus_tag="PXD71_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDL6290194.1"
                     /translation="MAKQDSDCITLDLFATVPKVGRPRTNPLDREQQLRINKRNQLKR
                     DKSSGLKRVELKLHSNLVKLLEEQASEQGVSRGQLIETILNNYLTNR"
     gene            19271..20257
                     /locus_tag="PXD71_000021"
     CDS             19271..20257
                     /locus_tag="PXD71_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL6290195.1"
                     /translation="MSNIHLAIAADFTLAEKLLEALANQDLGIENISAVELEPFGEEQ
                     NLRFGAKAVAQYALDEVNWAEFSHVLFAGKMAQAEVLAEAVKAGCVILDLYGITALIG
                     DVPVVVPSVNDENVANLRERNIVALANPQISQLALALKPLLNEPLKQVWVTSLLPSAY
                     FGDEKVKELAGQTARLLNGIPFDENAERIAFDVVPANVQGEEKSLPFSKAFELQIAKV
                     LPNLTACTTFHSVQVPVFYGVSQMVSVQSEYQLDANALSEAWKAENWLNFNENSVITA
                     VKNGENEEQNALEISQLLAKSEQEVQFWTVADEQRFSLAFLAVKLLKLVLAY"
     gene            20354..21649
                     /locus_tag="PXD71_000022"
     CDS             20354..21649
                     /locus_tag="PXD71_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL6290196.1"
                     /translation="MLEKLFQLKAKGSNVKTEIIAGITTFFTMVYIVFVNPSILGVAG
                     MDTQVVFVTTCLIAAFGTIAMGLFSNLPIALAPAMGLNAFFAFVVVQKLGYSWQVGMG
                     AIFLGSVGLFLLTVLQVRYWFMSAIPLGLRVGIGAGIGLFIALIGFKNMGLVVPNPAT
                     LVALGDLHDPKVLMGILGFFIIVVLAAKGIHSGVLISIVIVTALALAFDPAVSFNGIM
                     SMPPSLGAVVGQVDIAGAFDVALLGMIFSFMLVNLFDSSGTLIAVTTKAGFADEQGRF
                     PKMKQALLVDSTAAMAGSYMGTSAISTYIESGSGVSVGGRTGLTAVVVGILFLLTIFF
                     SPLAGLVPAYATAGALVFVGILMASSLIEVKWDDLTEATPAFITTAMMPFTYSITEGI
                     AFGFISYCVMKAFTGRWKEINLSVLVVSLLFIAKFVWVG"
     gene            21769..22137
                     /locus_tag="PXD71_000023"
     CDS             21769..22137
                     /locus_tag="PXD71_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HDL6290197.1"
                     /translation="MSNSVQKLLFIINSAPYGNETFFSALRLALQIQEQHKANTEIKL
                     FLMSDAVTAGLAKQNPAEGYHIQQMLEILTAQGANVKLCKTCTNARGITDLPLADGVE
                     IGTLIELADWTIQADKVLNF"
     gene            22137..23330
                     /locus_tag="PXD71_000024"
     CDS             22137..23330
                     /locus_tag="PXD71_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL6290198.1"
                     /translation="MQNIDVVVIGAGAAGLFCAAQLGQAGKKVVVLDSGKKVGRKILM
                     SGGGFCNFTNLDVSAGNYLSQNRHFVKSALSRYTQWDFISLVSHYGISYHEKELGQLF
                     CDESSQQIVDLLQAECNKGKVEILLRQAVISVEKVANFFEISTACGKFQSEHLVIATG
                     GLSMPALGASPFGYQLAEQFGIPVVPPRASLVPFTWKESDKYFAELSGIALPITVSAN
                     GKSFSNQMLFTHRGLSGPAILQISNYWELGETVEIDLLPSQSITDILSELRQSSPKLQ
                     LKTVLSRYLPKKLVEVWLEQKLLTDKVIADLSKKDLTLLEQFIHHWHFLPNGTEGYRT
                     AEVTKGGVDTDYISSKTMEAKNTAGLYFIGEVLDVTGWLGGYNFQWAWSSAFACAEGI
                     IEKSK"
     gene            complement(23401..23619)
                     /gene="infA"
                     /locus_tag="PXD71_000025"
     CDS             complement(23401..23619)
                     /gene="infA"
                     /locus_tag="PXD71_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL6290199.1"
                     /translation="MAKEDCIEMQGTILETLPNTMFRVELENGHIVTAHISGKMRKNY
                     IRILTGDKVTVEMTPYDLSKARIIFRAR"
     gene            complement(23729..24388)
                     /locus_tag="PXD71_000026"
     CDS             complement(23729..24388)
                     /locus_tag="PXD71_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDL6290200.1"
                     /translation="MYISREKITKTLLLAGITLLCCGIVAVILMGVNQFKLGSQLASI
                     NQVSNLSHLLVRQQANIFSFLLVKNAKTDELVDALDSFAKEDFIIDANIYSPNGVLLA
                     QSQNAKDLKVLLDSEQSAKTQQIVEPIFSQQALVGFLRVTFDAEYGQTTKARVEQQFH
                     QLYGEIIILLLTGILLASSINYYFRRKIHHVQISPKPLMSQVKTQTQRFHSRRRWIKR
                     K"
     gene            24474..25337
                     /gene="serB"
                     /locus_tag="PXD71_000027"
     CDS             24474..25337
                     /gene="serB"
                     /locus_tag="PXD71_000027"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HDL6290201.1"
                     /translation="MTNTIFIYSKTLNEREIEQFSQQTAAILLKQAVYLDYKIAFFST
                     NLTACELRETAQKINADISDLTIIPTLNQGGLLLMDMDSTAIKIECIDDIAKLAGTGE
                     VVSAITASAMRGELDFEQSLRKRVGTLENASESILQKVRENLPLMDGFEQMVAILKEH
                     GWKLAIASGGFDYFADYLKQKYGLNFAVSNQLEIIDGKLTGKVLGKVVDAQHKADTLQ
                     NLATTFNIPPNQWVAVGDGANDLPMLKTASLGVALHAKPKVQAQADFVVNFGDLTALC
                     LLLNANHLFQH"
     gene            25353..25844
                     /locus_tag="PXD71_000028"
     CDS             25353..25844
                     /locus_tag="PXD71_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL6290202.1"
                     /translation="MPSFDIVSEITMHEVRNAVENANRVLSTRYDFRGVEAVIELNEK
                     NESIKLTTESDFQLEQLIEILIGSCVKRGIEHSSLDIPTESEHHGKLYSKEIKLKQGI
                     ESDMAKKITKLIKDSKIKVQAQIQGEQVRVTGKSRDDLQAAIQLVKGADLGQPFQFNN
                     FRD"
     gene            25929..26105
                     /locus_tag="PXD71_000029"
     CDS             25929..26105
                     /locus_tag="PXD71_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5363 domain-containing protein"
                     /protein_id="HDL6290203.1"
                     /translation="MMSEQTEKKGWFKKALEKYDAFCDELGVNQGACRGCVPVVKFDP
                     EPEKEQPEKSENKT"
     gene            complement(26153..26449)
                     /locus_tag="PXD71_000030"
     CDS             complement(26153..26449)
                     /locus_tag="PXD71_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290204.1"
                     /translation="MKKHHIIILTLASASVVACSSNTPVQVVNGDKNIDYSQPKTIYI
                     SETNTQPPALTKQEKIKPSNATALCFNGSYSTDIQNPCKKDGGVKQYFAHHHAN"
     gene            complement(26526..27494)
                     /gene="mdh"
                     /locus_tag="PXD71_000031"
     CDS             complement(26526..27494)
                     /gene="mdh"
                     /locus_tag="PXD71_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDL6290205.1"
                     /translation="MCQSKKVALLGAAGGIGQSLALLLKLNLPAKSELSLYDISPVTP
                     GIAVDLSHIPTDVKVTGFAGEDPTEALKDADVVVISAGVARKPGMTRADLFNTNATIV
                     HNLVEKAAKVCPKACIAIITNPVNTIIPIAAEVLKKAGVYDKNKLFGVTTLDVIRANT
                     FVAEAKDVNVKYVRVPVIGGHSGTTILPLLSQATVNGLKLEFTQEQIEQLTHRIQNAG
                     TEVVEAKAGGGSATLSMAQAGAEFALGLVRALIGEDVIRYAYVDNANGETSPAFFAYP
                     IRLGTNGVEKVLPIGNLSEFEKDQLEQLIPVLNDEIQLGQTFNKNA"
     gene            27690..28151
                     /gene="argR"
                     /locus_tag="PXD71_000032"
     CDS             27690..28151
                     /gene="argR"
                     /locus_tag="PXD71_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDL6290206.1"
                     /translation="MDKIDSLSEAFKSLLKEEKFGSQSEIVAALQEMGFDNINQSKVS
                     RMLSKFGAVRTRNPKMEMVYQLPVELSVPTTSSPLKNLVVDIDHNDALIVVKTSPGAA
                     QLIARLLDSMGKAEGILGTIAGDDTIFITPVRNVQIEKLVKNVTQLFETSL"
     gene            28201..29097
                     /locus_tag="PXD71_000033"
     CDS             28201..29097
                     /locus_tag="PXD71_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDL6290207.1"
                     /translation="MHIFITGGTGFIGRELIQQLKSENHHFTLLTRDTEAKSDDLQIN
                     YCYSLDELVDLNDFDAVINLAGEPIFDKRWTDEQKQKLIESRVNLTNKLSQLMNASSN
                     PPHTFLSGSAIGIYGDLPPSANFYTESTACYQDCFTNRLCQQWEAAALKAASDKTRVC
                     LIRTGTVFSPEGGALKKILPLYRCGLGGKLGSGKQYWAWISRRDHVQAVIFLLKNANC
                     SGAYNFVAPNPVRNEEFNRILANAVNRPAFFTVPEFALKLGLGERACLVLDNQQLIPE
                     KLLAASFVFQDPILTENVFLNP"
     gene            29142..31340
                     /gene="uvrD"
                     /locus_tag="PXD71_000034"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             29142..31340
                     /gene="uvrD"
                     /locus_tag="PXD71_000034"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDL6290208.1"
                     /translation="MDFSLLLDGLNDKQREAVAAPIGNYLVLAGAGSGKTRVLTHRIA
                     WLIGVENVPESNILAVTFTNKAAAEMRHRIEYTLSQSGDNRLFGMWVGTFHSIANRLL
                     RSHYLDADLPQDFQIMDTEDQQRLLKRLLKLHNIDEKHFPPKHVAWYINAQKDKGKRA
                     KDIDHYNDPNEKKLVEIYQIYQDACDRAGLLDFAELLIRAYELFKTKSTILQRYQQRF
                     QQILIDEFQDTNNIQYDLIRLLAGDVGKVMIVGDDDQSIYGWRGAQVENIQRFLNDYD
                     NAQTIRLEQNYRSTGNILSCANELIANNDNRLGKNLWTDSGDGEPVDIYCAFNELDEA
                     RFVASQIKQWKEDEGDLAECAVLYRSNSQSRVIEEALIQANIPYRIYGGMRFFERQEI
                     KDALAYLRLIANRQDDAAFERVINTPTRGIGDRTLDTLRQITRGRQITLWQAIQVAVQ
                     EEQLSGRATSSLLRFVELINALDQETEQMSLAEQTDFVIKKSGLYEMYKQEKGEKGEV
                     RIENLEELVTATKQFVKPDEAEELTDLTAFLTHASLEAGEAQASQHEDYVELMTLHSA
                     KGLEFPRVFIVGVEEGIFPSGMSFDEGRLQEERRLAYVGITRAKKKLTISYAESRRLY
                     GKEERHVPSRFIAELPEEHIREVRLRGSINRAASFNQANSFAKNPTKTTASFLNDDTW
                     KMGQKVNHAKFGQGTIINVEGSGEATRLQVAFVGNGIKWLIAKMANLERI"
     gene            complement(31487..31561)
                     /locus_tag="PXD71_000035"
     tRNA            complement(31487..31561)
                     /locus_tag="PXD71_000035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31527..31529),aa:Gln,seq:ttg)
     gene            complement(31616..31690)
                     /locus_tag="PXD71_000036"
     tRNA            complement(31616..31690)
                     /locus_tag="PXD71_000036"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31656..31658),aa:Gln,seq:ttg)
     gene            complement(31716..31800)
                     /locus_tag="PXD71_000037"
     tRNA            complement(31716..31800)
                     /locus_tag="PXD71_000037"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31764..31766),aa:Leu,seq:tag)
     gene            complement(31803..31879)
                     /locus_tag="PXD71_000038"
     tRNA            complement(31803..31879)
                     /locus_tag="PXD71_000038"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31843..31845),aa:Met,seq:cat)
     gene            complement(32046..32870)
                     /gene="aroE"
                     /locus_tag="PXD71_000039"
     CDS             complement(32046..32870)
                     /gene="aroE"
                     /locus_tag="PXD71_000039"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL6290209.1"
                     /translation="MNRYAVWGNPIAQSKSPRIHQLFALQTQRNIEYIANLGDETAFE
                     QQLADFFASGAKGCNITAPFKERAFKLADEFSETALLAQACNTLKRLEDGRLYADNTD
                     GAGLVSDLERLGWLQKGHKTLILGAGGATKGVLLPLLKAGLEITLYNRTQEKAVVLAE
                     KFAKFGKIQTACLSDLANQLFNLIVNATSLGLQGKYVELPESVIASANIYDMQYAPNM
                     QTPFLNYARRCGATACQDGLGMLVGQAAFAFSLWEGQFPDVEPVLAQLKLEMQTVK"
     gene            complement(32877..33431)
                     /locus_tag="PXD71_000040"
     CDS             complement(32877..33431)
                     /locus_tag="PXD71_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDL6290210.1"
                     /translation="MNNFLEIVNQLKQEEVVAYPTEAVFGLGCNPQSEKAVRKLLVLK
                     QRPEEKGLILLASDLKYLLPYIDEARMTEQAWQRLSHIGEQAITWIVPVKNTVPSYLT
                     GKFDSIAVRLCNVPAVVQLCEASGVALTSTSANLSGEPPCRNAKEVIDQFGQDFPVLH
                     ADTLGRENPSEIRDIFSQQIFRQG"
     gene            complement(33424..33960)
                     /locus_tag="PXD71_000041"
     CDS             complement(33424..33960)
                     /locus_tag="PXD71_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDL6290211.1"
                     /translation="MVLFKSTKQTETCPQCGSPLQIKQGKKGLFLGCSAYPECDYIKP
                     LHQAGHIIKQLDELCPECGGNLQLKQGNYGIFIGCSHYPECHFIVHEEAEVEEEFDCP
                     ECKQHKLIARKGRSGKIFYGCIGFPNCKFTLATRPINHLCEKCGCELATIKKIKGKQF
                     TICANKSCQHQTELKPNE"
     gene            complement(33970..34281)
                     /locus_tag="PXD71_000042"
     CDS             complement(33970..34281)
                     /locus_tag="PXD71_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL6290212.1"
                     /translation="MNREKITFFSRFEADILSGKKTITIRDKSESYFQPNQELAVFTN
                     ETDRFFANIKVLSVTPIVFDELNEQHAKQENMSLAELKQVIREIYPNDNAFFVIEFTL
                     I"
     gene            complement(34274..34750)
                     /gene="recX"
                     /locus_tag="PXD71_000043"
     CDS             complement(34274..34750)
                     /gene="recX"
                     /locus_tag="PXD71_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL6290213.1"
                     /translation="MTTSTTKTNKYTAVNYLLYLLSKRDYSEQELRQKLKQKEYELTE
                     IDAAIEKAQANQWQSDERFCTTFIRYRSMQGIGPRRLKQELKLKGVKDWLINQELENA
                     EVDWFELAEQIFEKKRPLVWDLKAKQKMWRFMVSRGFYNDHFSHLMALDYSSDEYE"
     gene            complement(34809..35915)
                     /gene="recA"
                     /locus_tag="PXD71_000044"
     CDS             complement(34809..35915)
                     /gene="recA"
                     /locus_tag="PXD71_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL6290214.1"
                     /translation="MAEKKSQKNTPVKQIDPEQKEKALAAALAQIEKQFGKGSIMKLG
                     DTQALDIEAVSTGSLGLDSALGIGGLPMGRIVEIYGPESSGKTTLTLSVVAQAQKNGK
                     TCAFIDAEHALDPIYARKLGVDTDGLLISQPDNGEQALEICDALVRSGAVDVIIVDSV
                     AALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKATNCLVIFINQIRMKIGVMFG
                     NPETTTGGNALKFYASVRLDIRRSGVVKDGDEVIGSETKVKIVKNKVAPPFREVQFDI
                     MYGEGIARMNELLILAESHGFINKAGAWFSYEGEKIGQGKNNAIKWLKEHPEVASKIE
                     QDIRNLLISNPTFTATPDSENADNADDEFSEEEL"
     gene            complement(36016..36636)
                     /locus_tag="PXD71_000045"
     CDS             complement(36016..36636)
                     /locus_tag="PXD71_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDL6290215.1"
                     /translation="MCRFPLFETLSIIDGKVQNLQYHQQRFEQATQEYFGCEPRFSLA
                     DILVVPEASQRGKVRCRIDYNATEFELKFFIYSPKKVERFRLVEVENWDYHLKFSDRK
                     RFDLLPILQNEEVIILNNGYVSDCSIGNLLFLKQGIWYSPQHYLLKGTQLSKLLDEGK
                     VVLAKIAKQDLHQYEKIMLINALNPFEEPRALPCSQIYFCDLDRKS"
     gene            complement(36614..37588)
                     /locus_tag="PXD71_000046"
     CDS             complement(36614..37588)
                     /locus_tag="PXD71_000046"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDL6290216.1"
                     /translation="MQDFIHQANHYGQAKRPFFFLIDFEQQKPLIFPLEQAHASGLFF
                     EFFCKSNLEKPVEKPTKAFELSANPIPFSEYQTGFELVKKEIQAGNSYLLNLSYPTAI
                     ETNYSLAEIFAASKAKYKCYLQGRFVCFSPECFVRIENNRIFSYPMKGTINANEENAE
                     QKLMASEKEFTEHNTIVDLIRNDLALVAKNIEVTKYRYVEKVETHRGAILQTSSEICG
                     ELGENWQENIGSILAKLLPAGSISGAPKEKTVSIIQQAELGKRGYYTGIFGYFDGESL
                     ESAVAIRYIAQTSNGLVFHSGGGITSQSVLTDEYNELLEKVYVPISAI"
     gene            37698..39305
                     /gene="rmuC"
                     /locus_tag="PXD71_000047"
     CDS             37698..39305
                     /gene="rmuC"
                     /locus_tag="PXD71_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDL6290217.1"
                     /translation="MHFQEIGIYIALAVCVLVGVYLFFLKSRYQRNAYELSQDLGKIT
                     ENFEQIQQKYEVLAQEKNQLEQWAIQQQTKYEAVSERLNERDGQLQRFQQKIELAEQQ
                     ENQLERYINELKERVGSSQAKAESLEEQLQFSQSSLSTKERENQALFSRLNEVQNELT
                     ELRTTLSEKQANFEAQQRNFIEVKQQLNVEFQHLAQQILDEKSKRFSETNQSSLEALL
                     KPFKEQIEGFQKRVNEVHSESLKGSANLEAEIKRVLQIGVSMSEEAQNLATALKGNNK
                     IAGNWGEVQLESALQSAGLLAGEHYQAQESFRDEEGRRFAPDFVVHLPDQKHLIIDSK
                     VSLVAYDQAVRSEENFAISQALDEHCRSLRNHIEGLSKKNYSALLGIKSPDFVLMFIP
                     IEPAYIEAMKHDPQLFNFGYERNVILVSHTTLMPILRTVANLWRIERGNSEAREISEK
                     AGEIYNQVCAVADRLAKLGNTLNTANNQYNQTVTSLVGRQGLLGKVERFQHLSSKASQ
                     IVPQVELIENEVDTIKLEMVSRELLEE"
     gene            39368..39952
                     /locus_tag="PXD71_000048"
     CDS             39368..39952
                     /locus_tag="PXD71_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HDL6290218.1"
                     /translation="MLKKLLIIDNHDSFTFNLVDLLRKLDVPAKVVLVEELDLNEVEH
                     FSHILISPGPDVPRAYLQTFAMLERYHQTKSILGVCLGHQTICEFFGGKLYNLNDVRH
                     GQQRTLTQIEPNPIFKGLPKQLKVGLYHSWGILQNSLQNTPLVPTAVCDQNVLMAFKH
                     QHLPIFGVQFHPESFITEFGQQMLANWLEITQDF"
     gene            40049..41131
                     /gene="prfA"
                     /locus_tag="PXD71_000049"
     CDS             40049..41131
                     /gene="prfA"
                     /locus_tag="PXD71_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL6290219.1"
                     /translation="MKDSIINKLESLSERHEELQALLGDASIISDQDKFRAYSKEYSQ
                     LEEVVGTFNRWKKLNNDIEEAQLLLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLP
                     KDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYK
                     EIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEIN
                     PSDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNKAKALAVLAS
                     RIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMN
                     GKIDELIQPIITEYQADQLAALSEQN"
     gene            41243..41845
                     /locus_tag="PXD71_000050"
     CDS             41243..41845
                     /locus_tag="PXD71_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290220.1"
                     /translation="MRKLGLLAVFTLVLSACSNSTKIEQTILSKQDIVGSWVCAIKYD
                     DLKVSTLDFSEFKANGEMTNFGDISDENFEPIKFTYVTGDKGKWDLKGNRLVIDYDLS
                     ERKVKRTTPKKFLEFLKQEERKASKTAKKLLKYEQGIFNILSDQKNTENSKIILDILK
                     FGHSRMAIQQNMGDKVYSGGCVTADKAEEYLDILKNRNSK"
     gene            41845..42711
                     /gene="prmC"
                     /locus_tag="PXD71_000051"
     CDS             41845..42711
                     /gene="prmC"
                     /locus_tag="PXD71_000051"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL6290221.1"
                     /translation="MDYREWLTFAEQKLLENSGADPYLNAKFDANLLLQAVTKRSKSA
                     IFAFSETLLSESELAELAEKLARRAKGEPMAYILGYREFWSLPLKVSPATLIPRPDTE
                     RLVELALEYANKRLGNQKMLQILDLGTGTGAIALALASELGEKAQIIGVDFQNEAVQL
                     AEENRRNLGLNQVTFLQSDWFSSLANQQFDLIVSNPPYIDESDENLTVGDVRFEPLTA
                     LVADNKGLSDLQKIIENAPLHLKPQGALMLEHGWKQGNLVRDLFDLHLWEQVETAQDY
                     AGNDRVTKAILK"
     gene            42720..43586
                     /locus_tag="PXD71_000052"
     CDS             42720..43586
                     /locus_tag="PXD71_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HDL6290222.1"
                     /translation="MDDIIEELLKELEDPKVTISETELKKFLYREILRLTTLIDKNVS
                     EQQVFGQMSALVKKARYNVNGEGDEERINQLLHLVYREWGFHCYYEDYFHTENLLLNQ
                     VIRKRRGMPVSLGAVALYLSAVLDLPLYPVNFPTQLVLRAEIHQPNGATKVRFINPWN
                     GEFLTIEMLNKWLEGEMGFGVEVTPDLLRRAEPQELLERVETVFKMALTREGKYEETL
                     RLIEYRLAFSPEDPYEIRDRGMVLASMDCYQAALEDINYFIDQCPEDPSAEILKMEVK
                     GLQQKSQESVVH"
     gene            43676..44530
                     /gene="kdsA"
                     /locus_tag="PXD71_000053"
     CDS             43676..44530
                     /gene="kdsA"
                     /locus_tag="PXD71_000053"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDL6290223.1"
                     /translation="MTDKIVKVGNIEVANHKPFTLFGGMNVLESRDMALRVCEQYVEV
                     TQKLNVPYVFKASFDKANRSSIHSYRGPGMEEGLKIFQELKQTFGVNIITDVHEIYQC
                     KPVAEVVDVIQLPAFLARQTDLVEAMARTGAVINVKKPQFLSPGQMGNIVEKIAECGN
                     DQVILCDRGTNFGYDNLVVDMLGFGIMKKVSKGCPVIFDVTHSLQCRDPFGAASGGRR
                     DQVTELARSGMAIGLAGLFLEAHPDPNSAKCDGPSALPLSKLEAFVSQMKAIDELVKS
                     FEEIDTSK"
     gene            44751..45698
                     /locus_tag="PXD71_000054"
     CDS             44751..45698
                     /locus_tag="PXD71_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDL6290224.1"
                     /translation="MLNIVFLDRTGIPATHDIPRPNFPHTWTEYATTSADQTFERVKE
                     ADIIITSKVLLQRDLLAKLPKLKLIAITATGTNNVDLAAAKELGIAVKNVTGYSSVTV
                     PEHVIGMIYSLKHRLADYHRDLITSDRWATCGQFCYVDYPIHDVRGSTLGIFGKGNIG
                     AEVARLAQAVGMQVIFAEHQGATEIREGYTAFEEVFKQADIISLHCPLTEQTQNLINA
                     ETLALMKPTAYIINTGRGPLIDEQALLEALEQGKIAGAALDVLVKEPPEKDNPLMLAA
                     KRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVATGM"
     gene            45796..46968
                     /locus_tag="PXD71_000055"
     CDS             45796..46968
                     /locus_tag="PXD71_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HDL6290225.1"
                     /translation="MNLTPFFIAFRYFRSKSADRFGRLVTNLASLGIVLGVMALVIVL
                     SVMNGLENMQKTNLLSTLPHAIITPQEGHFAKGEKLALPEFAKQSVVINRANVIIQSS
                     QGINAGQLIGVENATDDPLLVAENIPALLPSGEFKVVIGSRLANKLNVTVGDKLRLMI
                     TENSQYTPLGRVPVQRLFEISGVYQSNREASEFTLFANLADVGRLLRISEDQVQGVRL
                     YLQDPFQVTELSQYFDAERYQISDWREQKGEFFQAVKMEKNMMGLLISLIIVVAISNI
                     VTSLSLMVVDKQGEIAILQTQGLTKKQVMQIFVFQGAIVGVIGSIIGGLLGVLIAYNL
                     DQIILLLNPSIHLPTLISGTQIAVIVGVSMLLSLLCTLYPAYRASKIEPAQALRYE"
     gene            46985..47668
                     /gene="lolD"
                     /locus_tag="PXD71_000056"
     CDS             46985..47668
                     /gene="lolD"
                     /locus_tag="PXD71_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDL6290226.1"
                     /translation="MTELLRCEHISKFYDEGEQKVQVLKEVSFSMNEGELVAIVGSSG
                     SGKSTLLHTLGGLDQPSTGEVWIRGQSLQQLSSDKLALLRNQNLGFVYQFHHLMADFT
                     ALENVMMPMLIGKQNKTEAANRAEKMLQAVGLAHRMTHRPSALSGGERQRVAIARALV
                     NNPALVLADEPTGNLDQKTTESIFELIQQLNQEQNIAFLLVTHDLNLANKLSRSLVMR
                     DGVLSEGSL"
     gene            47665..48930
                     /gene="lolE"
                     /locus_tag="PXD71_000057"
     CDS             47665..48930
                     /gene="lolE"
                     /locus_tag="PXD71_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDL6290227.1"
                     /translation="MSTPFFISWRYQRGKQKNKLVSLIALFSNIGIALGVAVLIIGLS
                     AMNGFERELNNRVLSVVPHAELVSFQGNQPQPIFDSKKLEALIKKSQNVTASSPFVSF
                     TALIENGAQLKIAQVRGVDPKKQDQASQLSQFIPPEQWQAFNQQFQADEDGLILGAGI
                     AKALEVEAGDEVTLLIPQPTEDGKLAQPLRFNLNVTGILRLDGQLDHSYALIPLNKAQ
                     ELMEYQANEVSGLEISLANPFDVQQLEMPQLNGYTQPLYLNTWIDKFGYMYNDIQLIR
                     TVMYIAMVLVIGVACFNIISTLIMAVKDKQGDIAILRTLGANNGFIRRIFLWYGLISG
                     MKGALFGLILGVILSLNLTAIIKAIEAFFEVKLLSDGVYFVDFLPSELHWQDVVYVLA
                     ATIILSLFASLYPANRAAKLEPAKVLSGH"
     gene            complement(49005..50936)
                     /gene="thrS"
                     /locus_tag="PXD71_000058"
     CDS             complement(49005..50936)
                     /gene="thrS"
                     /locus_tag="PXD71_000058"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL6290228.1"
                     /translation="MPIITLPDGSQRSFDNPVSVMEVAQSIGAGLAKATIAGRVNGER
                     RDASDIISQDSTLEIITAKDEDGLEIIRHSTAHLLGHSIKQLFPDVKMAIGPTIENGF
                     YYDIDLDRSLTQEDLDALEKRMLELAKTNYDVVKRPVSWQEARDTFEKRGEPYKMAIL
                     DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRI
                     YGTAWADKKQLAEYLQRLEEAAKRDHRRIGKALDLYHMQEEAPGMVFWHNDGWTIFRE
                     LETFVRTKLKEYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQSENREYAIKPMNC
                     PGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IESEVISCIKMVYDIYSTFGFTNIAVKLSTRPENRIGDDAMWDRAEKGLANALRNNGL
                     EYEIQEGEGAFYGPKIEFALRDCLGREWQCGTVQLDFALPGRLAASYVAEDNGRRTPV
                     MIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQQVVKQLSDAG
                     IRVKADLRNEKVGFKVREHTLRRVPYMLVCGDKEIAEGKVSVRTRKGADLGTYLVSDL
                     VEILKNQIKARELKLLGEE"
     gene            complement(51103..52452)
                     /locus_tag="PXD71_000059"
     CDS             complement(51103..52452)
                     /locus_tag="PXD71_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6290229.1"
                     /translation="MNSTKFYSLYSGIFFLFFGYGLFLNSAGIKLAEMGITDIIIGLL
                     NAAFFIGAALSTIFAHRIVSSVGHIRSFSVFCAVFAIAALGHIMIDTLWIWGVLRIIL
                     GFCHYSLLLLVESWLSEKTNVDTRGKVLATYNIIFYLAFILGVSLLSLELSSRNIFIL
                     STILVVMSMIPIALTKMVQPDIPARERISIPKLFAISPLALATSFISGMLVNGFFTMA
                     SVFLLKFSFSLSEISLYLIISMIGGFISQMPMAILSNRIGRRNAILACSVIATLTALA
                     GLFAIFQENLTAWGQYIIAFFFGCSLFSLYALSIARANDELPNNMNTVEVSRGLLFCY
                     GVGALVAPPLIGFAMSLSAEYGFYALFGLFSLSLFICAFRTKSIPKAERAEMQYATPI
                     ATASPLEEIEVVEDALIPFDEEAVQEYQAQLEILEQDPQTENENIELDEGAAENSKV"
     gene            complement(52727..53218)
                     /locus_tag="PXD71_000060"
     CDS             complement(52727..53218)
                     /locus_tag="PXD71_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL6290230.1"
                     /translation="MFTTQLIQTKRWLEANSITTFWLKTFIGATLLAIFAQISVPMYP
                     VPITGQTLALTVVGFAMGRKAATAAVLLYLFEGAIGLPVFAGGASGFHHFFGPTGGYL
                     LGYIPTAYFLGYYSDKGVLNSFWKSLFVALIASVITFAFGLAQLSLYQKAQYYKRAYI
                     HLF"
     gene            53299..54066
                     /locus_tag="PXD71_000061"
     CDS             53299..54066
                     /locus_tag="PXD71_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HDL6290231.1"
                     /translation="MVKPVYIYCDEGSSEIGVQSLLLAVKQHLQRPAYLINAKQIIQS
                     KFSDGCLLIMPGGADLPYCKKLNGEGNRRIRQFIEQGGAYLGICAGAYYGCAELNFVG
                     RDYSVNGNRELALFNGIAGIAKGSLPQFTNGQLYDKNIGSKAIVEVTLAEGGKMPFYY
                     HGGCCFEPNSTTVIYNPLAYYPDGSVAMIEGELGRGYYLLSGVHFELQAVVYWQKIVN
                     CCENLDIKTQEEAICSNFNVNYGSQIWQHLKQRLNQA"
     gene            complement(54057..54851)
                     /locus_tag="PXD71_000062"
     CDS             complement(54057..54851)
                     /locus_tag="PXD71_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL6290232.1"
                     /translation="MRFFDTHTHLDYLAESLNLSIPELVENAKQHQVECILIVAVFAK
                     NFAKVTACAKQKPENLVYGLGLHPLYIQQHQQQDLILLENLLSQQDPQCTAIAEIGLE
                     KAVSEVSTPELWKKQCEFLEAQLSLAKKFNLPVNLHSRKSHDQLFVFLKKAQLTKTGV
                     VHGFAGSYEQAKRFVDLGYKIGVGGTITYERANKTREAIRKLPLESLLLETDSPDMPV
                     FGFQGQPNRPERLLVTFNSLCELRTESPNLIAETIWQNSERLFFYA"
     gene            complement(54912..56498)
                     /locus_tag="PXD71_000063"
     CDS             complement(54912..56498)
                     /locus_tag="PXD71_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDL6290233.1"
                     /translation="MLWFFFCVAVLVLGYFTYGKIIEKIFVINPKRATPAYAMNDGVD
                     YMPMSKTKIWLIQLLNIAGTGPIFGPILGALYGPVAMLWIVIGCIFAGAVHDYFCGML
                     SIRNGGATMPNLAGKFLGRPVKLFINVMALILLLLVGVVFVASPAQLLSTITMDIAGA
                     TQSAMELGDVEAIKESVASGGVTVWGMDKATVISVWTLIIFAYYILATLLPVDKIIGR
                     IYPFFGALLLFMSVGMVYGLVSAHLSATDPIDFFRTVNADGQGLTWEKFTQNFQVKGD
                     VPIWPLLFLTISCGALSGFHATQTPLMARCAQNEKEGRFIFYGAMITEGVIALVWCAV
                     GLSFYENPQALQDAISAGSPSKVVYDSSLHFLGFIGGIFAVLGVVVLPITSGDTAFRA
                     ARLQLAEIFGLEQRTLVKRLYIAVPLFVLGFIVSKVDFSVLWRYFTWANQMTAMVMLW
                     TAAGYLYRYKKFHWVCSIPAWFITTVCATYLANNKIGFGLDYELSVYIGFAITVVCVI
                     CFFAFLKPSEARDEDAYVTN"
     gene            complement(56684..58537)
                     /gene="dxs"
                     /locus_tag="PXD71_000064"
     CDS             complement(56684..58537)
                     /gene="dxs"
                     /locus_tag="PXD71_000064"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL6290234.1"
                     /translation="MEKKYPLLSEINSPEDLRLLSKDQLQTVVDELRAYLLESVSQTS
                     GHLASGLGVVELTVALHYVYQTPFDQLIWDVGHQAYPHKILTGRRDQMHTIRQKDGIH
                     PFPWREESPYDVLSVGHSSTSISAGLGIAVAAEKENAGRKTVCVIGDGAITAGMAFEA
                     LNHAGALHTDMLVILNDNEMSISENVGALNNHLARIFSGSLYTSVRDGSKKILDKVPT
                     IKNFMKKSEEHMKGVIFSPESTLFEELGFNYIGPIDGHNIDELVKTLSNMRELKGPQF
                     LHIRTKKGKGYTPAENDPIGYHGVPKFDPTAAELPKSKAAPTYSNIFGDWLCEMAERD
                     PKIIGITPAMREGSGMVEFSKRFPEQYFDVAIAEQHAVTFGAGLAIAGYKPVVAIYSS
                     FLQRAYDQLIHDVAIQNLPVIFAIDRAGIVGADGQTHQGAFDLSFMRCIPNMTIMAPS
                     DENEMRKMLYTAYTMNTPSAIRYPRGNAKGVKLEPMQRLEVGKGNIIREGKKVAILNF
                     GALLDEAKIVAEKHNYTLVDMRFIKPLDEALLQKVADSHELLVTLEENAIQGGAGSFV
                     NEYLQNIGKIKPLVMLGIPDFFVPQSTQAEAYADLGLDAQGIEAKIQKFDI"
     gene            complement(58582..59394)
                     /gene="suhB"
                     /locus_tag="PXD71_000065"
     CDS             complement(58582..59394)
                     /gene="suhB"
                     /locus_tag="PXD71_000065"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDL6290235.1"
                     /translation="MNPMLNIAIRAARKAGNIIAKGYEQSPQDTQVLQKNTNDYVTEI
                     DKAAEAAIIDVIRKSYPEHSIVTEESGVLNGSNENVQWVIDPLDGTTNFVKRLPHFAV
                     SIAVRENGRTTVGVVYDPIRNELFTAVRGEGAKLNEFRLRIDAERRDLKGTVIATGFP
                     FKVIRHRDAHLNMLEGLMNNGVADFRRTGSAALDLAYVAAGRVDGFFEIGLKPWDFAA
                     GELIAREAGAIVTNFVGGTDYLKTGNLVVGNGRVVKEILNSIQPTLTEELKA"
     gene            59575..60609
                     /locus_tag="PXD71_000066"
     CDS             59575..60609
                     /locus_tag="PXD71_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HDL6290236.1"
                     /translation="MQFKENNQPRFKHREERRSDECSPRFSDKGKMRGNEGKSDRPHK
                     LHFKRDEAYSRSEQIYPAEGKKASGEGSVQIWVKGGNSSVKEKKNGPLSPRAPEKIKK
                     NRLEEMKVYGENACISLFQQRPESIVRLWATVEGAKKLGDLMSYLAQHKKAYHVIDRE
                     EMERVTGTEHHGDMCLLVKKTASFVLEGYLQVPKKQDCLVLLDGVNNAQNVGGIIRTC
                     AFYGVKGIITENVDSLNSSAAARVAEGGLEFVRALETKNKHIALVQLRQAGYQIVHLT
                     RHKQAPSLTKIKLAEKVVFVLSEIPASDVAYAEDTTIQLSFNNPLNSGLNVAVNAGIL
                     LNQWYQTHIL"
     gene            60619..61503
                     /locus_tag="PXD71_000067"
     CDS             60619..61503
                     /locus_tag="PXD71_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDL6290237.1"
                     /translation="MNQQKPFEVIAIVGTPRHDSAFETHLAVYNWLKDRDYQILVETE
                     VATQLQLKESYSLDQIGQRAELVIVIGGDGNMLGIARALAKYRVPLIGINRGNLGFLT
                     DIAPQTAFEQLYSCLVKNEYVIEERFLLEAFIERNGKIIAANNALNEIVVHPTQVARI
                     IEFEVYIDGKFAFSQRADGLIVATPTGSTAYSLSAGGPILTPNMNAMALVPMHPHTLS
                     SRPLVVDGDSQISLRFAKYNTPDLEVICDGQVNMHFTPEDRVLVRKSPDKLQLLHLKD
                     YSYFTVLGSKLGWASKLF"
     gene            61642..62619
                     /locus_tag="PXD71_000068"
     CDS             61642..62619
                     /locus_tag="PXD71_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1852 domain-containing protein"
                     /protein_id="HDL6290238.1"
                     /translation="MTTNFKCQVHTIEFNEDYQPEQSTRLTTNFANLARGENRKQNLQ
                     NAIAMINNRFNDLANWDNPNADRYSVKLDIVSLDIDVEGKGEFFPAIEWLKTYVIDYK
                     TGKTIDGIIGNNFSSFVRDYDFSVLLLDHNKDKPQFSVPEGYGELHGKLFKHLLASPE
                     YTAKYKKPPVICLSVATTRTYTRTTNVHPILGVEYLQDSYSLTDAYFAKMGLKVRYFM
                     PPKSVAPYAFYFDENADLLNDYTNLELISTIATMEAFQKIYRPEIYNANSVAGQVYKG
                     SLTYQDYSVTKVEYDREERTQLAIKQGKYTEEHFIKRYQAILEAWATEE"
     gene            62707..63738
                     /locus_tag="PXD71_000069"
     CDS             62707..63738
                     /locus_tag="PXD71_000069"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL6290239.1"
                     /translation="MSNILLPTSTAGSLPKPSWLAEPEKLWSPWKFEGEELLQAKRDA
                     LLVSLAEQIAAGIDIVSDGEQTRQHFVTTFIEHLEGVDFSKREIVRIRNRYDASVPSV
                     VGAVSRPKSVFVDDAKFLRAHTDKPIKWALPGPMTMIDTLYDGHYKSREKLAWEFAKI
                     LNEEAKELEAAGVDIIQFDEPAFNVFFDELNDWGVAALERAAEGLKCETAVHICYGYG
                     IKANTDWKQTLGNEWRQYEESFPKLQQSKIDIISLECQNSRVPMDLIELVRGKKVMLG
                     AIDVATNIIETPEQVADTLRKALQFVDADKLYPSTNCGMAPLSRQVAQGKLQALSQGA
                     AILRAEFAK"
     gene            63826..64047
                     /locus_tag="PXD71_000070"
     CDS             63826..64047
                     /locus_tag="PXD71_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290240.1"
                     /translation="MLNLFKKPIKKSSFEAWAKLMDDVAKVAILAIPVILYGEYSLLM
                     KFTHSITLFAVVYFLLATARLFRETAEEM"
BASE COUNT        19181 a        12812 c        13727 g        18432 t
ORIGIN      
        1 gaagcaaatg gaacaactgg atattacggt acttgaaaat gccttcaatt cccttgagaa
       61 aacgattgtc agccttgaga atcaagcgtg gtttaaccgg caagaagcaa ttattcaaga
      121 cactttgatt gcaggagcaa tccaaaaatt tgaatttgtt tacgaattgt cgataaaaat
      181 gctcaagcgt cagctcaaaa acattgagtc aaatgacact gatattgata gcaccgagtt
      241 ccgtgatatt ttgcgaatgg ctgctcaata tgggctgatt gaatcaccgg aagtttggct
      301 ggattatcgc aaaatgcgaa atattacttc gcatacttat gatcaaaata aagcacagca
      361 ggtttataac ggaatttcaa actttctgga aagtacgtat tttttagtta aacagctcca
      421 aaaacgcaat agctatgatt gatttaactg agtcacaacg ccaacaggtg ttggatattt
      481 tcaaaaaata cttgcctgat acacccgttt gggtttttgg ctctcgcatt aaaggcacag
      541 ccaaagccta ttcggatctt gatttagcat taatcaccaa acaaccgctc acaattcggc
      601 aacaaacaga gttagagctt gcgtttacgg attctgacct acctttcaca gtagatctga
      661 ttgactgggc aagttgcaac gaatcgttta aacaaattat tttacagcga tatgaagtgc
      721 ttatttaatc gctaaatatg ctataatttc cccaattttt aacttttgtg acaccaatga
      781 aaaaattttt tcttctttta attgcttttt ttactttaag tgcttgtgaa aaagcccctg
      841 agcaaattac gctgtcaggc aaaaccatgg gaacgactta caccgtaaaa tatattgata
      901 atggtgaagt ggagaatttg atttcgcctg aaaaaatgaa aaatgagctg gatggcttat
      961 tgattgaggt gaataatcag atgtccacct accaacaaga ttcggaaatt agccgtttta
     1021 atcaagcaaa gcaggcaaat caagcggtcg aaatttcgca ggattttgca aaagtggtac
     1081 aagaggcaat ccgtttaaat aaagtaaccg aaggggcttt agatgtaacc gttggtccat
     1141 tggtgaactt gtggggcttc gggccggata aacgcttaaa taaagtgccg tctgatgaac
     1201 aaattgcaga gcgttctgct tatgtcggca ttgataaaat tttgcttgaa atgagtagta
     1261 aacccaagct caccaagtca gcagctaatc tttatctaga tttatcgtct attgcaaagg
     1321 gtttcggtgt agataaatta gccgagcatc ttgaaactat aggtgtgagc aactatttag
     1381 ttgaaattgg tggcgaatta cgaggcaaag gaaaaaattt acaaggctta gattggcgga
     1441 ttgcaatcga gaagccggta atgacacaag gtcaagccgc acaaattacc gtgccgttgc
     1501 acaatttggc gatggcaact tcgggcaatt atcgtaatta ttttgaagat gaaaaaggca
     1561 accgtctttc acatattatc gacccaaaac agttacgtcc ggtaaaccat aatttagcct
     1621 ctatcaccgt atttgcccca actacaatga cggcagacgg cttatcaacc ggtttatttg
     1681 ttttaggagc ggaaaaagca ttagcagtgg cagagcgtga aaaattagct atttttttaa
     1741 ttgttaaaaa cggcgataaa tttgagacac aaatgtcgag cgaatttagc aaacttattc
     1801 aatcacaata gttggagtga aaaatggaaa ctgtattatt aacctttggc ttttttgtag
     1861 ccgtaatttt tgcgatgtcg attggtttta tcgtgaaagg caaaaccatc aaaggcagct
     1921 gtggcggtat caccgcttta ggtatgaaaa aaatgtgcga ctgcgaagaa ccttgcgata
     1981 acctaaaatc taaaatcgca gacggcacag ccgaccctga agaagtggca cgttttaata
     2041 aagagccaca attttatgaa gtgaaataat atgaatagag atattcgctg gaaacagcgg
     2101 tttgataact ataaaaaatc agtaagttac cttcaagcag aagcagaaaa atacgctgat
     2161 accgacattg atgtaattaa aaaaggaatt atccagagtt ttgagattac acacgagtta
     2221 gcgtggaagt taatgcaaga tattttaaaa tgggaaggtg aagttgatat ttatggttca
     2281 aaaacggctt ctcgtttggc ttttaatcgt gggctaatca ctcagggcga agtttggcta
     2341 gaaatgataa aaagccgcaa tataacagtt catactgacg atgaaactat tttaactgaa
     2401 gaatttaata aaattatcaa ggcgtatctg cctttattta ttgaatttga acagcgagta
     2461 gaaacactat gccaaacttt ggactaagcg aacgagcgac gaaaactatt accgatattc
     2521 ttgcgaaata ccctgaagtg gagcaagcca ttatttacgg ctcgcgtgct aaaggtaatt
     2581 atcgtgaagg ctcagatgta gatcttactt ttaaaggaaa agatcttact tacgatattg
     2641 tcaaacgcat ttggggtgat ttagacgatt ccgactcgcc ctatttattt gacttgtcga
     2701 tttatcatca attaaccaat gccaacttta ttgaacatat tgatagagta ggaaaagtct
     2761 tttaccaaag agaactctaa tttcttaata ggcaagcggt tcattttgca aaattttttg
     2821 caaatttcac cgcttgttgt ttttattatt aaccaatgtg cttcgcctta accgaagtta
     2881 cgggagattt atgacaactc aattaacctc aaaaacctac gacactcatt tccctaaatt
     2941 aaccccagaa caacttgctg aaaacagcaa gaaaaaagtc attatcggta tgtccggtgg
     3001 ggtggattcc tcggtttccg cttttattct tcaacagcag ggctaccaag tggaaggctt
     3061 gtttatgaaa aactgggaag aagatgatga tacggattat tgtaccgccg ccgccgagct
     3121 tgccgatgct caagcggttg ccgataaact gggtatgaaa ctgcataaaa ttaattttgc
     3181 agcggaatat tgggataatg tatttgaata tttcttggca gaatacaagg caggaagaac
     3241 gccaaatccg gatattttgt gtaataaaga gatcaaattt aaagcctttt tggaatatgc
     3301 ggcagaagat cttggtgcgg attatattgc gaccgggcat tacgtccgcc gtacgagcga
     3361 tgatcaagat gcgaaacttt tgcgtggctt agatgaaaac aaagatcaga gttacttttt
     3421 atatgcgtta aataaaaagc agatcgggca gagtttattc ccagtgggtg aaattgagaa
     3481 gccgattgtg cgagccattg ccgaagacct aggattagca accgcaaaga aaaaagattc
     3541 taccggcatt tgttttatcg gtgagcgtaa atttaaagat ttcttagctc gttttctacc
     3601 ggctcaaccg ggggaaatcc gcaccgttga gggcaaatta gtcggtcgcc atgatgggct
     3661 gatgtatcac acgcttgggc aacgcaaagg cttgggtatc ggtggggtga aagggctaag
     3721 tgaagatccg ttttatgtgg tggagaaaga cttaattaac aatgtattga ttgtggcaca
     3781 aggacacgat aattcagccc tgctttcaag tggcttaatt gcgaaacagc tcacttgggt
     3841 ggatatgcag ccgttgcgtg aaaatttacg ctgcacggta aaaacccgtt atcgtcaagc
     3901 agatattgcg tgtgaaatcc aagtgattga tgatgaaacc attcgagtga tgtttgatga
     3961 accgcaaatt gccgtgacgc cggggcagtc tgccgtgttt tacttaggtg aagtttgctt
     4021 aggcggtggc attattgagt cacaattaaa atagttataa ataaatattg aaatattttg
     4081 catatttatg caaaataagc gaaaagttaa ggcggtaaaa cgcctttttc attttgatcg
     4141 atacaaagaa taatatacaa ggaacagact gtggcacttt ggggtggacg ctttacacaa
     4201 caagcagacg caaaatttaa atattttaac gattcattac gttttgatta ccgcttggcg
     4261 attcaagata ttgaaggctc aatcggctgg gcaaaagcca ttacttcggt aggtatttta
     4321 acggtagcag aattagaaca actaatcgca gcattaaaag aggtgagagc agaggtagaa
     4381 tctaacctta ccatcatctt aaaagatgat gcggaagaca ttcacagctg ggttgaatct
     4441 aagctgattg aaaaagtggg cgatttaggt aaaaaattgc acaccgggcg tagccgtaat
     4501 gaccaagtag cggtggatat taaaatgtgg tgcaaggtgc aagcggtggc attgcaagaa
     4561 agaatccgag ctttgcaaga gcgtttggtt tccgttgccg aagaaaatca agagtcggtg
     4621 atgccgggct atacccattt acaacgagct cagcctgtga ccttcgctca ttggtgtatg
     4681 gcgtattatg aaatgctaga acgggatttc agccgtttga gtgatgctaa taaacgtatg
     4741 agcacttgcc cgcttggttc aggggcattg gctggtacgg cttacggcat tgatcgtgac
     4801 ttattagccc gtgatttagg ctttgaagaa gccacacgca acagtttaga cagcgtgtca
     4861 gatcgtgacc atattttaga gctgctctct actgcctcga tcagtatgat gcacctctct
     4921 cgctttgctg aagatttaat catttttaat agtggcgaat caggcttcct tgaaatgtcg
     4981 gatcgtgtga cctcaggctc atcacttatg ccacagaaga aaaaccctga tgcttgtgag
     5041 ctgattcgtg ggaaatccgg tcgggtattt ggggcattaa acggtttatt aactacactc
     5101 aaagggttac cacttgctta taataaagat atgcaagagg acaaagaggg tatttttgat
     5161 gcgttggaaa cttggcaggc ttgtttagaa attgcagaac ttgtactggt tgatattcaa
     5221 gtgaataccg aacgcacccg agaagccgct cagcaaggtt atgcgaatgc gaccgagttg
     5281 gcagattatt tagtggcgaa aggcgtgcca ttccgtgaag ctcatcatat tgttggcgaa
     5341 gcggtggttt acgcaatcag caaaaaagag ccacttgaag cattaagcat taacgaattc
     5401 cagcaattcc atcattcgat tggcgatgat gtttacccaa ttctctcgtt agaatcttgc
     5461 ttagaaaaac gttgtgccaa aggtggagtg aacccacaac gtgtggcaga ggcgattgcg
     5521 gtagccaaag ccaatttagc ttcaaagtaa aaccggatac aagcggtcat attttgcaaa
     5581 aaaattgaaa aatatgaccg cttgccgatt aaattttcaa ttttttacgc attaagattg
     5641 caaaataggc attacctctt taaacttccg aataattttt gaattttatt tctactactt
     5701 tctttaaaga gttttatatg acggaatcag ttaattctac tcaagctggt acagcacttt
     5761 ctttagggca acaattaaaa gcggctcgtg aagcgttaaa tctctctatt acagatgttg
     5821 ctcaaaaaac caacttaaaa aaatcacata ttgaatcact tgaaaatgat atctttattc
     5881 tacaaaatgt tgccccaact tttgtgcgtg gctatgtgcg taattatgtg aaatttttgc
     5941 gtttacctga atcccttatt tcgagcgtta attatggtga agtcactatt ccaaaagaag
     6001 tcactaaagt ttcttcagta aaaccttcat caaattcaca aggtaaaacc ttaaagtatt
     6061 taacggtatt agtattgctt gcggcattag gaatgacatt actttggtgg tggcaagggt
     6121 atcaaaaaga acaggaaaat cgcgagcaat ttgttagctc tgcaccagtt gctgaagtgg
     6181 cgaataccgc tacaaataat gatgcttcag cacaaacttc cgtgcctgtt cagcaaccta
     6241 ttcaggtttc agagcagcct gctcaagtag cagaaacggt agttgcagca gaaccggcat
     6301 taactgaaaa agtagagcct acacaagcag catcatcacc tgtaacacaa gaagatgtta
     6361 aagtggtaga agaagttaaa caagctgatg ccaccactgc acaggttgag gcagttaatg
     6421 ttttacagca atctcaatcg gaacaagccc ctacagaaca gcctgttgcg gtaacgaatg
     6481 atgagcttcg tatcgaaatt accggttcac aaagctggat tacagtgcgg ggtgctaaaa
     6541 ataaacgttt agcagaaaaa ttatataaca atggtgagac tttaagtttc aatgagaatg
     6601 agcaatatcg tttaaccatt ggtgcgcctg ccaatgtaaa agtgtactat aaaggtcaag
     6661 aagtaccatt gaaaattgat gggcgtgtag ctcgtattca tttaccgtta cagtaagctt
     6721 gatttaacga tgaaacgcac gtaaagctag catttaacga tagctgtctt tacgagcgtt
     6781 ttctgttttt ataaattttc acaattagct aaaaatatga tacatcaatc acctattaaa
     6841 cgtcgggtat cgaagaaaat ctatgtgggt aatgtgcctg taggcggcga tgcaccgatt
     6901 acggttcagt caatgaccaa cacccgcaca accgatgtgg aagccaccgt tgctcaaatt
     6961 aaatcgctgg aacgggtcgg ggctgatatt gtgcgtgtgt ctgtgccgac aatggacgct
     7021 gctgaggcat ttaaagcgat taaacaacaa gtgagtgtgc cattggtggc ggatatccac
     7081 ttcgattacc gcattgcgtt gaaagttgcc gaatatgggg tggattgctt gcggattaac
     7141 ccgggtaata tcgggaatga agagcggatc cgcacggtgg tagattgtgc cagagataaa
     7201 aacattccga tccgaatcgg ggtaaatgcc ggatcattag agcgtgattt gcaggaaaaa
     7261 tatggcgagc cgacaccaca ggcattatta gaatcggcat tacgccacgt tgatcactta
     7321 gatcgcctta actttgatca attcaaagtc agcgtgaaag cctcagatgt atttctagcg
     7381 gttgaatctt accgacttct tgcaaaacag attgatcagc ccctgcattt aggtattact
     7441 gaagcaggcg gagcgagagc cggttcggtg aaatcggcgg taggcttagg tctgttgcta
     7501 gcggaaggga ttggtgatac cttgcgtgtg tcattagcgg ctgatcctgt tgaagaggtg
     7561 aaagtcggtt ttgatatttt gaaatcgctg cgtattcgct ctcgtgggat caactttatt
     7621 gcttgcccaa cctgctcccg ccaagaaatt gatgtgattg cgacggtaaa cgagctagaa
     7681 caacgtttag aagacattat cacgccgatg gacgtttcga ttatcggctg tgtggtaaac
     7741 ggtccaggag aggcgttggt gtctgattta ggggtaaccg gcagcaataa aatgagcggc
     7801 ttttatttag atggcgttcg tcaaaaagag cgttttgaca acgcaaaatt aatcgaccaa
     7861 ttagaagcta aaattcgtgc cagagttgca caacaacata atcgtataga tattgaacaa
     7921 ataggataaa tatagtggca aaaacaattc aagcaattcg aggaatgaac gactgctcac
     7981 caagtgagtc tccattatgg caatgggttg aacagaaagt gcgtaatgtg ctggcaagtt
     8041 acggctattc ggaagtacgt atgccgattg tagaaagcac tccgttattt gcccgtgcca
     8101 ttggcgaagt aaccgatgtg gtgtcgaaag agatgtacac gttctgggat aacgatgagc
     8161 aattaacgct ccgcccggaa ggtacagccg gttgtgtgcg tgcagcgatt gagcgtggtt
     8221 ggatctacaa taatgagcaa cgcttatggt atatggggcc gatgttccgc cacgaacgtc
     8281 cacagaaagg gcgttaccgc caattccacc aagccggtgt tgaagtgttt ggcattgcaa
     8341 atccggaaat tgatgcggag ttgattattc tcaccgcacg cctatggaaa gagttaggca
     8401 tcgatcaaca tgtttctttg caacttaact ctatcggctc gttagaagct cgtgcaaatt
     8461 atcgctcagc attagtaaaa tttttagaaa atcacaccgc tttaatgaac gaagaagaga
     8521 aagagcgttt ggtgaaaaac ccgctacgga ttttagatac caaaaacgaa gcactacaaa
     8581 atgtattaaa cgatgcccca aaactgctcg attatttaga tgatgacagc cgtgaacatt
     8641 ttacccaact ttgtgcctta cttgatgcaa tgggaatccg ttacgaagtc aatccaaaat
     8701 tggtgcgtgg tttagactat tacaataaaa ccgtgtttga atgggtcact tcagccttag
     8761 gctcacaagg caccgtatgt ggcggtgggc gttatgacgg cttagtcgaa caactcggtg
     8821 gacacgcaac ccaaggcgtt ggctttgcaa tgggcttaga gcgtttagtg ctgttggtac
     8881 aagaggtgaa taaagagatt gatttaccaa aaacggtgga tgtgtatatc gtattctccg
     8941 gtgaaggggc aacactaaat gccttccaac ttgcagagca gattcgtagc gaattgccac
     9001 acgttggcgt aatgacacac tgttccggcg gtaaattcca aaaacaattt aagcgtgcag
     9061 ataaagtaga agcgcaaatt gcgttagtga ttggcgaaag cgaagtggct gaaaagcaag
     9121 ttgttatcaa agatttacgt tcaggagcag aacaaatcac gattgctcaa tcagagctga
     9181 ttgctgagct atctaaacga ttttaggaga aaaagatgag taacgaatat ttaaataaaa
     9241 ccgaagagca gcaatttgaa gatgccaaaa attggtttaa agagaacggg acaccgattt
     9301 tggtggcgat tattttagct tgtgcggcaa ctgccggctg gaatttttgg aaaaatcacc
     9361 agcttgaaac ggcacaaaaa acatccgctt cataccaaag cgtgatggag agttatttgc
     9421 aaaatccaag caaaaatcaa ccgcttgttg agaaatttat tgccgataat aaagattcaa
     9481 gctatgccgc ttttgcccaa tttgaagtag ctaaacaagc ggtcgaaaaa gggaattttt
     9541 ctgcggcaaa aacagcgtta cagtcagttt tgacttattc ggattcaacc ttacaatcta
     9601 tcgctcgttt tcgtttggct caaattgact tccaactaaa atcttacgat gaagcactgg
     9661 caagtctggc tcaaattcaa gataaagcgt gggaattgcg taagcaaatt ctaacaggcg
     9721 atattcttgc agcaaaaggc gatattcctg cggcgaaaag tgcttatgag caagctaaaa
     9781 cgaccgcaaa tcctcaggaa caaaccttaa ttgatgtacg tttgaataac ttataaaaac
     9841 aaagcctgat tgaaataaaa tcaatcaggc ttttttgttg gtttagtgtt gctgtgaatt
     9901 ataaacgcat tggcataatc acatattcgg cagagctgtt gtcgcaatct tcaattaagc
     9961 aactagaact tgaatcaact aagctaaaac gcacacgttg gcattttaag gtgtttaaca
    10021 catctaatac atagctaacg ttaaagccga cttccatttc cgggctgtcg taggcaacat
    10081 caatgatctc ttccgcctct tcttgctccg gattgtttgc ggtaattttg agtaaattat
    10141 tgcttaaggt taaacgcacc cctctaaatt tctcattaga aagaatcgct gcacgcatta
    10201 atgcccgttt aagggtttcg gtttccgctt ctagaatgcg atctgcattg cgaggcagaa
    10261 ctcggcggta atccgggaat ctgccatcaa tcagtttaga ggtaaataca atgccgttca
    10321 ttgtaatacg gatattgctt gaaccgattt ccaaacgcac ggtttcatcg ttgctggcaa
    10381 ccaagcggga aagctcaagt acggctttgc gtggcacgat aaccgaatga gtcaaaagct
    10441 cttgatttag caccattgtg caaaccgcta aacggtgccc atctgtggca acggtgcgta
    10501 ataaattgcc ttcggtttca aatttcattc cgtttaagaa ataacgggcg tcttggtttg
    10561 ccattgagaa ttgggtggca tcaatcagac gggcaagggt agattgatca atcgagaaat
    10621 ccacttccgg tttccaatcc atcaaattcg ggtaatcact tgccggtaat gtagctaaac
    10681 taaatttact acgtcctgct ttgataatgg ctttatcttc ttcaaattgt accgaaatca
    10741 cgctgtcatc aggtaggctt ttactgatat ctaggaattt tttagctgga atggtaaatt
    10801 ttccggcaaa atcgaccgct tgtagcagtt gtgcttgggt ggttaattcc acttctaaat
    10861 cggtaccggt taaagataga cgattacctt caatttctaa taaaagattg ttaataacag
    10921 gcaaagtcgg acggctactt aatacgccac acacctgttg taaaggctta agtaattgtt
    10981 ctctggtaat ggtaatttgc ataatcattc cttcatcggg ctaagatgat aatttgcgtg
    11041 ttaaatggat ataatcttct tggataccgc tttcggtatc gcgtaattca ttaatggttt
    11101 tgcaggcgtg catcacggta gtatgatccc gaccgccaaa ttcccgacca atttccggca
    11161 agctgtggtt ggttagctct tttgccagtg ccattgccat ttggcgagga cgggcaatgc
    11221 ttcttgcccg acttttcgat ttaatatcgg caacttttat gccgtaatat tcggccacaa
    11281 tgcgttgaat attttcaatc gtaatcaagt aatcaaaaga ggcaaacaga tcttgcaagg
    11341 tttcacgcac cgcatcaacc gtaatcgggc gaagggtcaa tttttgccac gcaatcacac
    11401 ggtttaatgc tccctccaat tcacgcacat tggttcggag tttttgcccc attaaatagg
    11461 cggcatcttc ttgcaaatct gctccacgtt ctaaggcttt ttggcgaaga attgccactc
    11521 gggtttccaa ttcaggtggc tcaatggtcg cactaatacc gccgcttaaa cgggatttaa
    11581 tcgctgtgtc aattttatcg atattttttg gcaaaacgtc agaggttaga ataatctgct
    11641 ttctctgctc aaataaagca ttgagtagat gtaaaaactc ttcttgtact ttcggtttat
    11701 tggcaagaaa ttgaatatcg tcaatcatta acacatcaag cgagcggtag aattttttga
    11761 ttttttccgc atcgttattg gaactgttga tcgccttaat aatatcacga taaaaacgtt
    11821 ccaaatgaat atatagcacc cgagtacccg gtgtgcgttt aactaattca ttgccgatag
    11881 cgtgtaataa gtgggttttg cccaaaccgg tgccggcata aaacgagagt gggttgcaat
    11941 ggctttcgcc taaattatcc accacttgtt gagccactaa tttagctaat tggtttgagc
    12001 gaccttgtac aaaattatca aaggtttgat ttggatttag cccggtttta aagccggtat
    12061 tgctgaattc tgaactctcg ttttttagat ttgggctgac ctttggctgt tctgtcggtt
    12121 taatcccaac ttttaacgta accaccaaat tatcattttt cgccaaaaaa cgggcaagct
    12181 cggtaatttc cgataaaaat ttatctttta cccagttttc aacaaattga ttttgtgcat
    12241 aaagtgtaag ttccccattc tcagaactcg cttgcagagg acgcagccaa gtgctgtaat
    12301 cagttggtga gactttcgtt tgtaagtgat taaggcaatc agaccaaagg gaagacacaa
    12361 tttgctccaa aattattgtt attaaaggtc ataatgatac ctgaaaaaag gggagaaatc
    12421 ttgcaaattt ttgagagatt ttatgggggc ttggaagcga ttttaattgg cgaacaagcg
    12481 gtcgtttttt gttgaaatct tgcaaattgc taacaaaaaa tgaccgcttg cttagtatga
    12541 tgtgtgatgg agttgattaa atatccgtat tctccggcaa gaacccaccg ctttggtgag
    12601 cccaaagtct ggcgtaaaca ccgttttgag caagcaattc ttcgtgcgtg ccttgttcaa
    12661 cgatttcgcc tttatctaac acgataagtc tgtccattgc cataatggta gagaggcggt
    12721 gggcaatcgc aaccacggtt ttaccttcca ttaattgcgt gagattttct tgaattgcca
    12781 cttctacttc agagtctaac gcactggttg cctcatcgag taataaaatc ggagcgtctt
    12841 ttagcatcac tcgggcaatc gcaatccgtt ggcgttgtcc gccggaaagt tttacccctc
    12901 gttcgccaac gtgtgcatca aaaccggttc tgcctttggc atcttgcagg tttggaataa
    12961 attcactggc ggcagcttgt tcaacggctt tgatcatctc ttcttcagtg gcgtttgggc
    13021 gaccgtacat caagttttca cgcacagagc ggtgtaacag cgaggtgtct tgagttacta
    13081 aaccaatttg tgagcgaagg ctttcttggg taatatcacg gatattttgc ccgtcaatcg
    13141 tgattgaacc gtcttgaata tcgtaaaaac gcaataataa attggtgagc gttgatttac
    13201 ctgcaccact acgcccgact aagccgactt tttcccccgg ttttatagtg aggtcaaaat
    13261 ctttcagtag cggttttttc ggatcgtagg caaaatccac attctcaaat ttaatttcgc
    13321 ctttggttac atttaacggt ttcgcatcga ttttatctac cacattgtgt ggtttggaaa
    13381 gcgtaatcat accgtcttgt accgtaccta aattttcaaa tagacgagca aattcccaca
    13441 taatccattg tgataagcct ttaatgcgta aagccaaagc ggttgaggtg gcaatcgccc
    13501 ctgcagttac tacgcttgaa ccccaaagcc ataaaccgat acccgcagtt gaaattatta
    13561 atgccatcga ggttagatta gttacggttt caattacagt cactaaacgc atctgtttat
    13621 gcactgtcac cataaatcct tccatcgact ctttcgcata ggcagattca cggttgccgt
    13681 gcgagaagag tttgacggtg gcaatgttag cgtaagcatc ggtaattcga ccggtcatta
    13741 aacttctggc atccgattgc tcttgagcag cttgggctaa tttcgggaca aagtagataa
    13801 tggttgccgc taatgagatg acccataaaa taaacggcac gaataaccag ccgtcaagtt
    13861 gtaataaaat cacgctggag gtggtgaagt aaaccagcac atacaccaac atatcggcac
    13921 aagtcataat cacatcacgc acggaaaggg cggtttgcat tacttttgca gacactcttc
    13981 ctgcaaattc gtcttgatag aaacctaaac tttgcccaag cattaagcgg tggaagttcc
    14041 aacgcaaacg cattggaaat accccttgca gagattgaaa gcgaatgctg ctggcaaggt
    14101 aaacaaacac cactgcaagt agggcaataa aaaacatacc gataatcgtc cagccttttt
    14161 ctgcccaaag ttctgttggc gagtatttat tgacccaatc gactaaatcg cccataaagc
    14221 gaaacagaac cgcttcgata ataccgacag ctgcaactaa tacggtcagt agtaagatat
    14281 aaaaccgcat tcctttagtg gcatcgtaaa taaaaggaat gagacctgat tttggagttt
    14341 tcggcatttc atttggatag gtttccaccc ttgcctcaaa ccatttaaac agtttttcaa
    14401 acataaattt gtgatttccg aataaaaatg caggggcaaa tagccatttg cccccgaaaa
    14461 aatgtgaaaa aattatggat taagtaattt ttcgtctaag gctaaatctt cgttgcgatt
    14521 tataccaatg ccggcagcta atacgttgcg ggcaatttga tgtgcttctt ttaaagaatg
    14581 ctctttgtaa gagccacatt gatattcatt cagctcaggg attgccgata catcaggaat
    14641 tttatgtaac gcatcttcca ttgatgccgt ccaagctttg acaacttctt ctgctgaagg
    14701 cgatccaatc agtgacatat aaaaaccggt acgacaaccc attggtgaaa tatcaatgat
    14761 ctccacttgt tcgctattga ggtgatcacg cataaaaccg gcaaaaagat gttccatcgt
    14821 gtgaatccca cgcaccggta gaatatcaat attcggacga cagaaacgga gatcgaacac
    14881 ggtaatggta tcgcctttcg gtgtggtcat tgtttttgca acacgcacgg ctggtgcgtt
    14941 cattttggtg tgatctactt taaaactatc taataaaggc attttttact ccatcaatat
    15001 tattccgcaa ttccttgatc ccaagccggg gcttcatcag gtagtttgcg ctgtaagaaa
    15061 aatcgtctgt ttggctgggc ttgaggcttc accactttag tgtaaggggt agagaactca
    15121 ataccgccat ttaataactg cgacattgtg ccggagttca tacttacacc ttgcaggctc
    15181 acgtccatag tgtaacctga agcttgccag aattgggtat tggttctgac taagtgcttg
    15241 tatttatctt gaatattgac ataaataagc actcgatcgc ctaactcact tagccctaaa
    15301 cggctgacta ttcccacctg cattccacgg taaagcaccg gtgcttccgg ctgaataccg
    15361 ttagcattgg tggtttcgac aataatgggg aaaccgttgg cataggtggt ttttaccgta
    15421 tcggtatctg caagaacaaa ttggttttga gcttgccctg aaccgacatc aaccgtaatg
    15481 taattttgta gtgctgcatc taaattttta aagccggagg tactgatttc aggcgagata
    15541 acattaaatt tactgccggc ttttgcaatt aacggatagt atttcctttg aatgtaagcg
    15601 gtcgctttta tctgtttttt tgcattttgt agctcaagtt tttcaatttg acctacggtt
    15661 aagcccatat atttaatgtc cattccttcg cttaacttac tcgcatcttt tgcaatcagc
    15721 gtgagataag tattgcccga agtggctttc tccttacttg cataaagcgt tttattgcct
    15781 tttgaaccgc catcatcaaa gctgatcgcc ccttttaagg tacgcattaa tggagctgta
    15841 gtgatattca cgcctttgag tgacacatca acactcgttg ccggctcaat ccagaaacgg
    15901 cttttctcgg ttaatagatg acgataattt ggctggataa atagctcaac ctcaaatttt
    15961 tgttgtttcg gggttatttt taacacctcg ccgacctgaa aatcacgata tagcaccaaa
    16021 gagccttcgc tgataccaga taatgaatct gccgaaaggg tagtggtggc ttttttatct
    16081 aaggtagcca tgcctgattc cgcactttct atatctttat aaagtgcata ttgttttttc
    16141 gggctaccgt catttttacc gttaattaag cgaacaccgc cttttaacca ttcagatggt
    16201 gaaccggcgt gaactcttaa cccatctagc cccaccgaca tatcaatgtt ggatatcgca
    16261 acaaatttgc tgttactccc aatcaggttg cgataaggcg gataaataat gacatcaaaa
    16321 cgtactttgt ctaactctag gctacgtttg acgatttctc cgatttgcac atcgctatag
    16381 taaatcccct gattggtatc taccccatag gcttgaggag caattaagct aaagctaagt
    16441 aaattcggta aacttaatag ataatcggct tcgttttgta ccacaaattc cgattgaggt
    16501 tctccttccc ccgggtgaac ctcaaaatag acaccacgca gcagctcacc tactttcgtc
    16561 agctgctctg tgtttaagga gaatttcggc tctttgagca agattttagt ttgggaacgt
    16621 agtaaatttt cataacttgg gtcaattaat aaaaccccat caatctgttt atgctgccct
    16681 gattcttgct gtggcacacc gttgtctttt acttgcggta attttagctg cgaaagcata
    16741 ccaatttgca cattttggtg aaaaacacca gtctcattaa tttttaaatt atgtgtaagc
    16801 ggtagtttta cgttgatttc aattccacgt ttagctgatt tcaaatccgg atatagctca
    16861 aatttctgtc cttgctcggc aatttccata ttatcaggcg aatcaaaagc aaccgcacct
    16921 tgtactaccg aggcaaggct gtccatattg attttaatac cttgtaaatt tacatcagcg
    16981 ctaataccgc taatattcca aaatcggctc tcttttttta ctaaatgagc gtatttttga
    17041 ttaatgacga catcaatttc gatttttttc tggtctttgg taaaacgata atcggcaata
    17101 ttacccactg gcactttacg gaaataaaca ctcgctccga cagtaataga gcctatgtca
    17161 tcagagatta aacggatcag taaatcgcca tcgctcacgg taatagccgg aggatcttct
    17221 tctgcaataa attcatcaac gaaatcgcct tcaccgggaa gtagggtaat atagttacca
    17281 gaaacaatcg catctaagcc tgaaacgcca gccaaagaag cacttggttg cactaaccaa
    17341 aatttagtct gtttgcgtaa aatacttttg gcttcagggt ttatttctgc ttgcacttcc
    17401 acttctttta aattatcgac aaaataaaca cgcttaacct gaccaatctg caagccttgg
    17461 taacgaatag cggtttttcc ggcagtaatg ccatcacctt tactaaatcg aatcgtgatt
    17521 gtttcacctt gctcttttaa aatttggaaa aatagcaaag caccaataat aaaagcaact
    17581 attggtaaca gccaaaaagg agaaattttg cgaggctgcc ggatttgggc atctacaaca
    17641 gtgtgggtat tttcttccat cgtcatttta aattagggaa acctatatag aacaataccg
    17701 aagacccaaa gggacttcgg taatagaaat aagtcaaaat aagaaatatt attttttctc
    17761 atcacagttg gttaaattac tgcatttacc atacagatat aaactgtggt tagtcaattc
    17821 cattccgtgt ttttcactga tttcacgttg gcgacgctcc atatcaggat ctttaaattc
    17881 gaacacctta ccacaatcca tacaaataat atggtcgtgt tcgtggttca tatttaattc
    17941 gaatactgct ttattcgcat cgaaattatg gcgggttaaa atgccggctt cttcaaattg
    18001 gtttaagaca cgataaaccg ttgctaaacc aatgtctgag cctttttcta acagaatctt
    18061 ataaatatct tctgctgcaa aatgttgcat ttcatcacga atatcttgca ttaatgcaag
    18121 gatagtcaaa cgaggctcgg ttacttttaa gcctacctct tttaaacgtt taatatttgc
    18181 ttcagacata tatgccactt atttatcttt atccaaaata gttattatgc gagttggtct
    18241 aagcacattt cttctttcaa ctgctcgcac catttattaa cacgttcagt ggttaattca
    18301 ggctgacgat cttcatcaat acataagccg acaaaagtat catcatcaac taaggcctga
    18361 gaaacctcga aagtgtaacc ggcagttggc caatgaccaa caatcacacc accatttggc
    18421 tctaccacat cacgcacagt acccattgca tcacagaaat attctgcata atcttcctga
    18481 tcaccgcaac caaaaattgc aacgactttg ccgttaaaat caatctcttt tagggtaggc
    18541 ataaaatcgt cccaatcggc ttgcgattca ccataatacc aagttgggat cccaaacatc
    18601 agaaaatcgt atgcttcaat atcttctttt gtgcttttgg caatgtctcg aatatcaatt
    18661 aattcagccc ctaatttttt ctgaatcatt tttgaaatat tttctgtatt gcctgtatcg
    18721 ctaccgtaaa ataaacctac aactgccatt gctactacct atttgtgaga taattattta
    18781 gaattgtttc aattaactgc ccgcgtgaaa caccttgttc agacgcctgt tcctctaata
    18841 actttactaa attcgaatgc agttttaatt ctacccgttt taaaccggaa gatttatctc
    18901 gttttagttg gttacgttta ttgattcgaa gttgttgttc tctatcaaga ggatttgtac
    18961 gaggacgccc aacttttggt acagtcgcaa acagatctaa ggttatacaa tctgaatctt
    19021 gttttgccat tttgttttac tattgggctt tagctaatcc tcagttttaa tgagaatgat
    19081 aagctacttt taaagtgtga actatatcaa aagttcaatc atttgaaaag taaattacag
    19141 gcaaaacaag cggtgttttt aggtttaaaa tttgcaaaaa ccataaatta cacattatga
    19201 gttatgattt ttctgaagcg tgaaattagc ttataatgaa accgaataac gttatgaaaa
    19261 aggagatagt gtgtcgaata tccatttagc gattgccgct gattttacgc tggcagaaaa
    19321 attacttgaa gcattagcaa atcaagattt agggattgaa aatatttcag ccgttgagct
    19381 tgagccgttt ggtgaagagc agaatttacg ctttggggca aaagcagtcg cacaatatgc
    19441 gttagatgaa gtaaattggg cggaatttag ccatgttcta tttgccggca aaatggcaca
    19501 agcggaagtg ttggcagaag cggtgaaagc cggctgtgtg attttagatt tatacggcat
    19561 taccgcactc atcggcgatg taccggtagt tgtgccaagt gtaaatgatg aaaatgtcgc
    19621 aaatttgcgt gagcgaaata tcgttgcttt agccaatccg caaatttcac aactggcgtt
    19681 agccttaaag ccattgttga acgagccgtt aaaacaggtg tgggtaacct cacttttacc
    19741 gtctgcctat tttggcgatg aaaaagtcaa ggagcttgcc ggacaaactg cccgtttgtt
    19801 aaacggcatt ccgtttgatg aaaatgcgga acgcattgcc tttgatgttg tgccggctaa
    19861 tgtgcaagga gaagagaaaa gtctgccgtt ctcaaaagcc tttgaactgc aaattgcaaa
    19921 agttttacca aatttgaccg cttgtactac cttccacagc gtgcaagtgc cggtctttta
    19981 tggggtttcc caaatggtgt cggtgcagtc tgaatatcag ttagatgcta atgcgttaag
    20041 tgaagcttgg aaagccgaaa attggctgaa ttttaatgaa aattcagtga ttacagcggt
    20101 gaaaaatggc gaaaacgaag agcaaaatgc gttagaaatc agtcagttat tggcgaaatc
    20161 cgaacaggaa gtgcagttct ggacggtcgc cgatgaacag cgtttcagtc tggcattttt
    20221 agcggtgaaa ttactaaaat tagttttagc gtattaagtg cttgcaaaag tatttattcc
    20281 gtgataaaaa tcgtcaacgc ccgagcaacc gattgcttgg gcgttgtcat tttgtttaaa
    20341 caccgaaggt cttatgttag aaaaattatt tcagctcaaa gcaaaaggca gtaatgtaaa
    20401 aaccgaaatt atcgccggta ttactacctt tttcacaatg gtttatattg ttttcgtcaa
    20461 tccgtctatt ttaggcgtgg cgggaatgga cacccaagta gtgttcgtga ctacttgttt
    20521 aattgccgct tttggtacta tcgcaatggg gttattcagt aatttaccga ttgctcttgc
    20581 accggcaatg ggcttaaatg ccttttttgc attcgtagtg gtgcaaaaat taggttactc
    20641 gtggcaagtg ggtatggggg cgattttctt aggctctgtc ggcttattcc tattaactgt
    20701 attacaagtt cgttactggt ttatgtctgc gattccactt ggtttacgtg taggtattgg
    20761 tgccggtatc ggtttattca ttgccctaat cggttttaaa aatatgggct tggtggtgcc
    20821 aaatccggca acgcttgtgg cactaggtga tttgcacgac ccgaaagtgt taatggggat
    20881 tttaggcttt ttcatcattg ttgtattagc ggcgaaaggt attcactcag gcgtgttaat
    20941 ttcaattgtg attgttaccg ctcttgcctt agcctttgac ccggcggtga gctttaacgg
    21001 cataatgtct atgccaccaa gccttggggc agttgtgggg caggttgata ttgccggtgc
    21061 ttttgatgtt gccttactcg ggatgatttt ctcctttatg ttggtaaacc tgtttgactc
    21121 ttccggcacc ttaattgcgg tgactaccaa agcaggcttt gcggatgaac aagggcgttt
    21181 cccgaaaatg aaacaagctc tgctagtaga tagtaccgct gcaatggcag gctcttatat
    21241 gggaacttca gcgatttcta cctatattga aagtggctcc ggcgtttcag ttggcggtcg
    21301 taccggctta actgcggtgg tagttgggat cttattccta ttaaccattt tcttctcacc
    21361 acttgcaggg ttagtacctg cctatgcaac tgcgggggca ttggtatttg taggaatttt
    21421 aatggcatct agcttaattg aagtaaaatg ggacgattta actgaagcta cgccggcatt
    21481 tattactacc gcaatgatgc cgtttactta ctccatcact gaagggattg cattcggctt
    21541 tatcagctac tgcgtaatga aagcctttac agggcgttgg aaagaaatca acctttctgt
    21601 tttagtggtt tcacttctct ttattgctaa atttgtgtgg gtgggttaag ctctaccccc
    21661 ctctccctga tttttcttct aaacgaagaa aaatctatcc ctctcccaca aaggggagag
    21721 ggtatttgat tcattatcta tgtgtttcgt gtattccgag gtaaaacaat gtctaattca
    21781 gttcaaaaac ttctttttat catcaattct gctccttacg gcaatgaaac gtttttcagt
    21841 gcgttgcgtt tggcattaca aattcaagaa caacataaag ccaatacgga aattaaactt
    21901 ttcttaatgt cggatgcagt caccgcaggg ctggcaaagc aaaatccggc agagggttat
    21961 catattcagc aaatgctgga aattttaacc gcccaagggg caaacgtaaa attatgcaaa
    22021 acctgtacta atgctcgagg gattacagat ttgcctttag ccgatggggt agagattggc
    22081 acattaattg aactggcaga ttggacaatt caagcagata aggtactgaa tttttaatgc
    22141 aaaatattga tgttgtggtg attggtgccg gtgcggcagg gctgttttgt gctgcccaac
    22201 tggggcaagc tggcaaaaaa gtggtggtgt tagacagcgg taagaaagtc ggacgtaaaa
    22261 ttttaatgtc tggcggtggt ttttgcaatt ttacgaattt agacgtaagt gccggaaatt
    22321 accttagcca aaaccgccat tttgtgaaat ccgccctttc acgttatacc caatgggatt
    22381 ttatttcgct tgtcagccac tatggcatta gctatcacga aaaagagcta gggcagttgt
    22441 tttgtgatga aagctcgcaa caaattgtcg atttactaca agcagaatgc aacaaaggca
    22501 aggttgagat tctgttacgg caagcggtca tttctgtgga aaaagttgca aatttttttg
    22561 aaatttcgac cgcttgtggc aagttccaat ccgaacactt agtgattgca accggagggc
    22621 tttcaatgcc ggctctcggg gcaagccctt ttggctatca acttgccgag cagtttggta
    22681 ttcctgttgt gccaccacgt gcaagccttg tgccttttac ttggaaagag agcgataaat
    22741 attttgccga gctttccggc attgcactgc cgattactgt ttccgccaat ggcaaaagtt
    22801 ttagcaatca gatgttgttt acgcacagag gcttatccgg cccggcaatt ttgcagattt
    22861 ccaattattg ggaattgggg gaaacggtag agattgatct gctaccttcg cagtccatta
    22921 ccgatatatt gagcgaacta cgccaatctt cgccgaaatt acagctaaaa accgtattat
    22981 ctcgttattt acctaaaaaa ttggttgaag tttggctgga acagaaattg ctgaccgata
    23041 aagtgattgc cgatttgagt aaaaaagatc tgaccttatt agagcaattt atccaccatt
    23101 ggcatttctt gccaaacggc acggaaggct atcgtacagc ggaagtaacc aaaggcggtg
    23161 ttgataccga ctatatttca tctaaaacga tggaagcgaa aaatacagca gggctttatt
    23221 ttatcggcga agtgctggat gttaccggct ggcttggtgg ttataatttc caatgggcgt
    23281 ggtcatctgc ttttgcctgt gcagaaggga ttattgaaaa aagcaaataa ctaaaaaagc
    23341 aaataactaa aaaaccaaac aaaaaggcga acacaattgt tcgccttatt ttatgctgaa
    23401 ttatcttgca cggaaaataa tgcgtgcttt gcttaaatcg tatggagtca tttcaaccgt
    23461 tactttatca ccggttaaaa tacgaatgta gtttttacgc attttgcctg aaatatgtgc
    23521 ggtaacaata tgaccgtttt ctaattccac acggaacatt gtatttggta aggtttctaa
    23581 aatcgtacct tgcatctcaa tgcaatcttc tttagccatt tattcctctt taaagtttta
    23641 atttttatat gatgccattc ggcgaaaaac ggcggatatt atacgcttat tcgctcaaat
    23701 tacaatcgtt tatccatttg agtgtaaact atttacgctt aatccaacgg cgacgtgagt
    23761 gaaaacgctg ggtttgggtt tttacttgac tcataagtgg ttttgggctt atctgtacat
    23821 gatgaatttt acgacgaaaa tagtagttaa tgctacttgc caacaatata cctgtgagta
    23881 gcaaaataat tatttcccca taaagttgat gaaattgttg ctctactcgt gctttagtag
    23941 tttgcccata ttctgcatca aacgtgacgc gtaaaaagcc aaccaacgcc tgttgagaaa
    24001 atattggctc aacaatttgc tgagttttgg cagattgctc tgaatcgagc aatactttta
    24061 aatctttcgc attttggctt tgagccagta gcacgccatt tggtgaatag atattggcat
    24121 caataataaa atcttctttg gcaaaactat ccaacgcatc aaccagctca tcggttttgg
    24181 catttttaac taacaaaaac gaaaaaatat ttgcttgctg gcgaaccaat aggtgtgaaa
    24241 ggtttgatac ttgattaatg ctggccaatt gtgagcctag cttaaattgg tttacgccca
    24301 ttaaaataac cgccacgatc ccacagcaga gtagcgtaat ccctgccagc aaaagcgttt
    24361 tcgttatttt ttcacgacta atgtacatcg aaattccctc aaaatgatgc gtgattgtag
    24421 cacgcttttc tgttagatta gtgcagtaaa tttaaacaac ctaaaataag acaatgacaa
    24481 acacaatttt tatctattca aaaaccttaa acgagcgtga aattgaacag ttttcacaac
    24541 aaacggcagc aattttgctg aaacaagcgg tctatttaga ctacaaaatt gcattttttt
    24601 ccacaaattt aaccgcttgc gagctgcgtg aaacggcaca gaaaatcaat gcggatatta
    24661 gcgatctcac tattattcca accttaaacc aaggcggttt gctattaatg gatatggatt
    24721 ccaccgctat taaaattgaa tgtattgatg atattgccaa acttgccggc accggcgagg
    24781 tggtgtccgc cattactgcc agtgcaatgc ggggcgagct ggatttcgag caaagtttac
    24841 gcaagcgagt cggcacgttg gaaaatgcat cggaaagcat tttgcaaaaa gtgcgggaaa
    24901 atctaccgct tatggacggc tttgagcaga tggttgctat tcttaaggaa cacggctgga
    24961 aattagcgat tgcgtccggt ggatttgatt acttcgccga ttacttaaaa caaaaatacg
    25021 ggttgaattt tgcggtctct aaccaactgg aaattattga cggtaaatta accggcaagg
    25081 tgttaggcaa agtagtggat gctcaacata aagccgatac cttacagaat ttagcgacaa
    25141 cctttaatat tccaccaaat caatgggtgg cagtcggcga tggggcaaat gacttaccaa
    25201 tgttaaaaac cgccagttta ggcgttgctt tgcacgctaa accaaaagtg caagcacaag
    25261 ctgattttgt ggtaaacttc ggggacttaa cggcattatg tttattactt aatgccaatc
    25321 atttatttca acattaattg agaggaacaa caatgccatc atttgatatt gtatctgaaa
    25381 tcacgatgca cgaagtgcgt aatgcggtag aaaatgccaa tcgtgtatta agcacccgct
    25441 acgatttccg tggggtggaa gccgttattg agttaaatga aaaaaatgaa agcattaagc
    25501 tcacgaccga atcggatttc caattagagc agttaattga gattttaatc ggctcttgtg
    25561 taaaacgtgg gatcgagcat agttcattag atatccctac agaaagtgag catcacggta
    25621 aactctattc caaagaaatt aagctcaagc aagggattga gtcagatatg gcgaagaaaa
    25681 ttaccaagct gattaaggat tctaaaatca aggttcaagc ccaaattcaa ggcgagcaag
    25741 tgcgtgtaac gggcaaatct cgtgatgatt tacaagccgc aattcagcta gtgaaagggg
    25801 cagatttagg gcagccattc caatttaata atttccgtga ttagcaattc ctgcaattaa
    25861 tgatgacaag tggaaaaatt cagcaaaatt tgaccgcttg cattaataat tcaacataaa
    25921 aggaagagat gatgagcgag caaacagaaa agaaaggctg gtttaaaaaa gcccttgaga
    25981 aatatgatgc cttttgcgat gagctagggg taaatcaagg tgcttgccga ggttgtgtcc
    26041 ctgtggtgaa gtttgaccct gaaccggaaa aagagcagcc agaaaaatca gaaaataaaa
    26101 catagaaaaa atgcacaatg gtaagataca ttgtgcattt tttataggat ttttaattgg
    26161 cgtgatgatg ggcaaaatat tgcttaactc cgccatcttt cttgcaagga ttctgaatat
    26221 cagtagagta actaccgtta aaacaaagag cagtagcatt cgatggttta atcttttcct
    26281 gtttagttag ggctggcggt tgagtattgg tttcactaat atagatcgtt ttaggttgag
    26341 aatagtctat atttttgtcg ccatttacga cttgaacggg ggtgttcgag ctgcaagcta
    26401 caaccgatgc tgaagccaat gtaagtataa tgatatggtg ttttttcatg ggtaatttcc
    26461 gtttttatat ttttaattga gagaatgtta aaaagggctg atttctcaac ccttaaagtt
    26521 atgccttaag catttttatt aaaagtttgt cctaactgaa tttcatcatt taatacagga
    26581 attaactgct ctagttgatc tttttcaaat tcgctgagat tgccgatagg tagaactttt
    26641 tccacaccat ttgtacctaa acgaatcggg taagcaaaga atgccggaga ggtttcgcca
    26701 ttggcattat ctacataagc ataacgaatg acatcttccc cgattaaggc tctcactaaa
    26761 ccaagtgcaa attctgctcc tgcttgagcc atagataagg ttgctgagcc accacctgct
    26821 tttgcctcaa ccacttcagt accggcgttt tggatacgat gagttaattg ctcgatttgt
    26881 tcttgagtga actcaagttt taagccattt actgttgctt gtgaaagcag tggaagaata
    26941 gttgtgccgg aatgtccgcc aatcacaggc actcttacat atttcacatt tacgtctttc
    27001 gcttcagcca caaaggtatt cgctcgaata acatctaatg ttgtaacacc gaaaagttta
    27061 tttttgtcat acacaccggc ttttttcaat acttctgccg caatcggaat aatggtgttt
    27121 accgggttag taataattgc aatgcaagct ttagggcaaa ctttcgctgc tttttcgact
    27181 aagttatgca cgatagtggc attcgtattg aataaatcgg cacgtgtcat ccccggctta
    27241 cgggcaacac cagcagaaat aacgaccaca tcggcatctt ttagtgcctc agtcggatct
    27301 tcacctgcaa aaccggttac ttttacatca gttggaatat ggcttaaatc cactgcaatt
    27361 cctggagtaa caggggaaat atcgtaaaga gataattcag atttagccgg taaattgagt
    27421 tttaataata aggcgagtga ttgaccgatt ccacctgcag cacctaaaag ggcaactttt
    27481 ttactttgac acataaaatt tctcctaaat gttatggaag tttagaccaa tttagtctaa
    27541 gccaattttt tgggcgattc aaacggcaaa tctcattttt gagacataga gcaaattttt
    27601 agctgatttt tatgggtttt attgatttat ttgcataaat aagcaagaat tatgaatatt
    27661 tttaagtttt tgtgaataag agtgtgaaag tggacaagat agatagttta tcagaagcgt
    27721 ttaaatcgct gttaaaggaa gaaaaattcg gctcgcaaag tgaaattgtt gccgcactac
    27781 aagagatggg ctttgataat atcaaccaat ccaaagtttc tcgaatgtta tcaaaatttg
    27841 gtgcagtcag aacccgtaat cctaaaatgg aaatggtgta tcagctacca gtcgaattaa
    27901 gtgtaccgac tacctccagc ccacttaaaa atttggtggt agatattgat cataatgatg
    27961 cgttaattgt ggtcaaaacc agccccggtg cggctcaact gattgcacgt ttgctcgatt
    28021 caatgggtaa agccgaaggt attttaggca ctattgctgg cgatgatacg atttttataa
    28081 cgccggtacg caatgtgcaa attgaaaaat tggtgaagaa tgtgacacag ttatttgaaa
    28141 catcattata gaataagtaa aggaggactt aatgttctcc ttatttttta ggagattatt
    28201 atgcacattt ttataacagg tggtacgggt tttattgggc gtgaattaat ccagcaatta
    28261 aaatctgaaa atcaccactt tacacttctt actagagata cagaagcgaa aagtgatgat
    28321 ttacagatta attattgcta ttcacttgat gaacttgttg atttaaatga ttttgatgca
    28381 gtaattaacc ttgccggtga gccgattttt gataaacgtt ggacagacga acaaaaacag
    28441 aaattgattg aaagccgggt taacctaacg aataagctca gtcaattaat gaatgcaagc
    28501 tctaaccctc cgcatacttt tctctccggt tcggcaatcg ggatttatgg cgatttgcct
    28561 ccttctgcaa atttttatac agaatcgacc gcttgttatc aagattgctt taccaaccgg
    28621 ctttgccaac agtgggaagc cgctgcacta aaagccgcaa gtgataaaac ccgagtgtgt
    28681 ttaattcgta ccggcacggt attttcgccc gaaggcggag cattaaagaa aattttaccg
    28741 ctttatcgat gcggattagg cggaaaactt ggttcaggca aacaatattg ggcgtggatc
    28801 agccgccggg atcacgtaca agcggtcatt tttctgctca aaaatgcaaa ttgttcgggt
    28861 gcttataatt ttgttgcacc aaatccggtg agaaatgaag aatttaatcg tattttagcg
    28921 aatgctgtta atcgcccggc attctttact gtgcctgaat ttgccttgaa attaggctta
    28981 ggcgaacgag cttgtttagt cttagataat caacagctca ttccggaaaa actactcgca
    29041 gccagttttg tattccaaga tccgatttta accgagaacg ttttcttaaa cccataattt
    29101 aggctataat gctgtataaa agcacagatt aaaaggtgat tatggatttc tcattattat
    29161 tagacggttt aaatgacaag cagagggagg cggttgctgc cccgattggt aattatttag
    29221 tgttagccgg ggcgggttcg ggcaaaactc gggttttaac ccatcgcatt gcgtggctga
    29281 ttggggtgga aaatgtgccg gaatccaata ttctggctgt tacctttacc aataaagcgg
    29341 cggcggaaat gcgacatcgg attgagtaca ctctttccca atcgggcgat aatcgattgt
    29401 tcggaatgtg ggttggtact ttccacagca ttgccaaccg cttacttcgt tcccactatt
    29461 tagatgcgga tttaccgcaa gatttccaaa ttatggatac cgaagatcag caacgtttac
    29521 tcaaacgttt actgaaactg cataatattg atgagaaaca ttttccgcca aaacacgttg
    29581 cgtggtatat caacgcccaa aaagataaag gcaaacgggc aaaagatatt gatcactata
    29641 acgatccgaa tgaaaaaaaa ttggtggaga tttaccaaat ctatcaagat gcctgcgacc
    29701 gagccggttt gttagatttt gccgagctgt tgattcgggc ttatgaacta ttcaaaacca
    29761 aatcaaccat tttgcaacgt tatcaacaac gttttcagca aattttgatt gatgagtttc
    29821 aagacaccaa caatattcag tacgacttaa ttcgtttgct tgccggcgat gtcggcaaag
    29881 tgatgattgt cggcgatgat gatcagtcta tttatggttg gcgtggggca caggttgaga
    29941 atatccaacg tttcttaaat gattatgaca acgctcaaac cattcgctta gagcaaaatt
    30001 accgctctac cggcaacatt ttaagctgtg ccaatgaatt aattgccaat aacgataacc
    30061 gcttaggcaa aaatttatgg acagatagcg gtgatggcga gcctgtggat atttactgtg
    30121 ccttcaacga acttgatgaa gctcgctttg ttgcctcgca aatcaagcaa tggaaagaag
    30181 acgagggtga tttggcggaa tgtgcggtgt tgtatcgcag caacagccaa tctcgtgtga
    30241 ttgaggaagc tttaattcaa gcgaatattc cttatcgcat ttacggtgga atgcgattct
    30301 ttgaacgcca agaaattaaa gatgctctgg cttatttacg cttaatcgcc aatcgtcaag
    30361 acgatgcggc gtttgaacgg gtgatcaaca cgccaaccag aggcattggc gatcgtacct
    30421 tagacacact ccgtcaaatt acccgtggtc gccaaattac gctgtggcaa gcaattcaag
    30481 tggcggtgca agaagaacaa ctttcagggc gtgccacttc ttccttattg cgttttgtag
    30541 agctgattaa tgctcttgat caagaaaccg agcagatgtc gcttgccgaa caaaccgatt
    30601 ttgtgattaa aaaatcgggt ttatatgaaa tgtataagca agaaaaaggc gaaaagggcg
    30661 aagttcggat tgaaaactta gaagagttag ttaccgcaac taagcagttt gtgaaacctg
    30721 acgaagcgga agagctgacc gatcttaccg catttttaac ccacgcctca cttgaggctg
    30781 gagaagccca agcctcgcaa cacgaagatt atgtcgaact gatgacgcta cattccgcca
    30841 aaggattgga gtttccccgt gtgtttattg tgggcgtgga agaagggatt ttcccaagcg
    30901 gaatgagctt tgatgaaggc aggttgcaag aagaacgccg tttggcttat gtcggcataa
    30961 cccgagctaa gaaaaaactg acgattagct atgccgaatc aagacggctt tatggtaaag
    31021 aagaacgcca cgttccgtct cgctttattg ctgaattacc tgaagagcat attcgagaag
    31081 tgagattgag aggctcaatt aaccgagcgg caagttttaa ccaagctaat tcatttgcaa
    31141 aaaatccgac aaaaacgacc gcttcctttt taaacgatga tacttggaaa atggggcaga
    31201 aagtgaacca cgctaaattt ggacaaggca cgattattaa tgtggagggc tcaggtgaag
    31261 ccacccgttt gcaagttgcc tttgtcggca acggcattaa atggctgatt gccaaaatgg
    31321 caaatttaga gaggatttag gcgaataaaa agttttttgc aggaatatag atggcgagat
    31381 atattgcccc ttaaccttaa actaagggta aataaaatta aataatgcaa gctagatata
    31441 atgtaaagta tcagaaataa gatattaatt taagataaag aatagatggc tggggtacta
    31501 ggattcgaac ctagggatgc cgagatcaaa acccggtgcc ttaccgcttg gcgatacccc
    31561 aaaagaaaaa actaacttac gttagcttat atagatttga atgtttgaaa ttaagtggct
    31621 ggggtactag gattcgaacc tagggatgcc gagatcaaaa cccggtgcct taccgcttgg
    31681 cgatacccca acactaattt ttgttaatcg ataaatggtg cgggacgagg gacttgaacc
    31741 cacacacctc gcggcgccag aacctaaatc tggtgcgtct accaatttcg ccagtcccgc
    31801 aatggtggct acagcgagat tcgaacttgc gaccccaaca ttatgagtgt tgtgctctaa
    31861 ccagctgagc tatgtagcca tttgcgttca ccatatcggt caacggagtg cattatgctg
    31921 attttagggc tttccgtcaa atactttttt taaaaaattt gaatttttga ttcgcttgta
    31981 tagttattcg tcgttttggc ttaaatctag cattttttgt ttataaaata atagcttata
    32041 ttctgctatt tcaccgtttg catttctaat tttaattgag ccagtacagg ctcaacatcg
    32101 gggaattgcc cttcccacaa agaaaacgca aacgctgcct gtccaaccaa cattcctaat
    32161 ccgtcttggc acgctgttgc tccacaacgc cttgcataat tgagaaaagg agtttgcata
    32221 ttgggggcat attgcatatc gtaaatattc gccgatgcta taacagattc aggcaattca
    32281 acatatttgc cttgcagacc gagagaggtg gcattgacga ttaaattaaa cagttggttt
    32341 gccaaatcgc ttaaacaagc ggtctgaatt ttgccaaatt ttgcaaattt ttcggctaaa
    32401 acgaccgctt tctcctgcgt gcggttatat agagtaatct ctaaccccgc tttaagtaac
    32461 ggaagtaata cgcctttggt tgctccacct gctccaagaa ttaaggtttt atgccctttt
    32521 tgtaaccaac ctagccgttc caaatcagaa actaaaccgg ccccgtcggt attatcggca
    32581 tataaccgtc catcttctaa gcgtttaagc gtattacacg cctgtgccaa caaagcggtt
    32641 tcgctaaatt catctgccaa tttaaaggct ctttctttaa aaggggcggt gatgttgcaa
    32701 ccttttgctc cagaagcgaa aaaatcggct aattgttgct caaaagccgt ttcatctcct
    32761 aagttagcga tatattcaat gttgcgttgg gtttgtaaag caaagagttg atgaatacgg
    32821 ggggatttac tttgagcaat cgggttgccc cacacggcat atcggttcat ttcggcttat
    32881 ccttgtctaa aaatctgttg gctgaaaata tctcgaattt cggaagggtt ttctctacct
    32941 agcgtgtctg cgtgtaatac aggaaaatct tgaccaaact gatcgatgac ttcttttgca
    33001 ttgcggcaag gtggttctcc gctcaaattg gcactggtag aggtcaacgc aacgccgctt
    33061 gcctcacaca actgaaccac cgccggcaca ttacataaac gtacggcaat agaatcaaat
    33121 ttgccggtga ggtaacttgg cacagtattt ttaaccggta cgatccaagt aattgcctgc
    33181 tcgccaatat ggcttaggcg ttgccaagcc tgttccgtca ttcgagcttc atcaatatag
    33241 ggaagaagat actttaaatc agaagccagt aaaattagcc ctttttcctc agggcgttgt
    33301 tttaatacga gtaatttcct taccgctttt tcactttgtg gattgcagcc taagccaaaa
    33361 accgcttcgg ttgggtaagc tacaacttct tcttgcttta attgattaac tatttctaaa
    33421 aaattattca ttcggtttta attctgtttg gtgctggcaa cttttgttag cacaaatggt
    33481 gaattgcttg cccttaattt ttttgattgt agcgagttca caaccacatt tttcacacaa
    33541 gtggttaatc ggtcgagttg ctaatgtaaa tttgcaatta gggaagccta tacaaccata
    33601 aaatattttg cctgaacgcc cttttctagc aatcagttta tgttgcttgc attcagggca
    33661 atcaaactct tcttcaacct cagcttcttc gtgtacgatg aagtggcatt cagggtaatg
    33721 gctacagccg ataaaaatac cataattgcc ttgttttaat tggaggtttc cgccacattc
    33781 agggcaaagt tcatctaatt gcttaataat atgaccggct tgatgtaatg gcttaatata
    33841 atcacattca gggtaagctg aacaacccaa aaataaccct tttttgccct gcttaatttg
    33901 caacggcgag ccacattgtg ggcaagtttc ggtctgtttg gttgatttga aaagtaccat
    33961 attgatttgc taaataagcg taaattcaat cacaaaaaac gcattgtcat tcggataaat
    34021 ctcccgaata acttgtttca attcggctaa gctcatattt tcttgtttag cgtgttgctc
    34081 gtttaattca tcaaacacta taggtgttac cgatagcact ttaatattgg cgaaaaatct
    34141 atcggtttca ttagtaaaaa ccgctaattc ttggtttggt tgaaaataag attctgactt
    34201 gtctcgaatc gtaattgttt ttttaccgct taaaatatcg gcttcaaatc ggctgaaaaa
    34261 ggtaattttc tctctattca tattcatcgc tactgtaatc cagagccatt aaatgactaa
    34321 aatgatcgtt ataaaaacca cggctaacca taaatcgcca cattttctgc tttgctttca
    34381 gatcccatac aagcggtcgt tttttctcaa aaatttgctc agctaattca aaccaatcca
    34441 cttcggcgtt ttctaattct tggttaatta accaatcttt taccccttta agttttagct
    34501 cttgctttaa acggcgaggc ccaatgcctt gcattgaacg ataacggata aaagtggtac
    34561 aaaagcgttc atcactttgc cactgattcg cttgagcttt ttcaatcgct gcatcaatct
    34621 ccgtgagttc atactctttt tgctttagtt tttgccgaag ctcttgctcg ctgtaatcac
    34681 gctttgaaag taaatatagc agataattaa ccgctgtgta tttatttgtt ttagtagtag
    34741 atgttgtcat ttatcggtta taaataaaaa tgcgattttc acgttgagcg aaaaccgcat
    34801 aaatttaatt agagttcttc ttcactaaat tcatcatctg cattgtctgc attttcagaa
    34861 tctggagtag ccgtaaaagt tgggtttgaa atgagtagat ttcgaatatc ttgctcaatt
    34921 ttactcgcca cttcagggtg ttcttttaac catttgatgg cattattttt accctgaccg
    34981 attttctccc cttcataaga gaaccaagcc cctgctttat taatgaagcc gtgagattcc
    35041 gctaaaatga gtaattcatt catacgggca atgccctcac cgtacataat atcaaattgg
    35101 acttcacgga acggtggagc aaccttgttt ttcacaattt tcacttttgt ttcgctaccg
    35161 ataacctcat caccgtcttt tactacgcct gaacgacgaa tatctaaacg tactgaagca
    35221 tagaatttaa gggcattacc accagttgtg gtttcagggt taccgaacat tactccaatt
    35281 ttcatacgaa tttggttaat gaaaatcacc aagcagttgg tcgctttgat atttgcggtt
    35341 aatttacgca atgcttgtga cattaaacga gcttgtaagc ccatatggga atcacccata
    35401 tcgccttcaa tctctgcttt tggtgtaagt gccgcaacag agtccacaat aattacatca
    35461 accgcaccgg aacgaactaa cgcatcacaa atttctaatg cttgttcgcc attatccggt
    35521 tgtgaaatta ataatccatc tgtatctacc cctaacttac gggcgtaaat aggatcaagt
    35581 gcgtgttcgg catcaataaa ggcacaagtt ttaccatttt tttgggcttg agccaccaca
    35641 gataaagtta atgtagtttt acctgaagac tccggtccat aaatttccac aatacgaccc
    35701 attggtaaac caccaatgcc taatgctgag tctaaaccaa gagaaccggt tgaaactgct
    35761 tcaatatcaa gggcttgagt atcgcctaac ttcataattg agcctttgcc aaattgcttt
    35821 tcaatttgag ctaatgcggc tgccaacgct ttttcttttt gttccggatc aatctgttta
    35881 accggagtgt tcttttgact ttttttctct gccatattct atcctttgta aacttttatc
    35941 taaaagcaac cgcttgttgc tgcaaaataa ctgtatatta ttatagtatt tacttatcca
    36001 gttgcaagca tttttttagg attttcgatc aagatcacaa aaataaattt gcgagcaagg
    36061 caatgccctt ggttcctcaa agggattaag agcattaatc aacataattt tttcatattg
    36121 atgcaaatct tgcttggcta ttttggctaa aacgactttc ccttcatcaa gcaatttact
    36181 aagctgagtg cctttgagca aataatgttg cgggctatac cagattcctt gttttaggaa
    36241 cagtaaattg ccgatggagc aatcactgac gtagccatta ttgagaataa tcacctcttc
    36301 attttgtaaa atagggagca aatcaaaccg cttgcggtcg ctaaatttga ggtgataatc
    36361 ccaattttcc acctcaacca agcgaaaacg ttctactttt ttgggcgaat agataaaaaa
    36421 tttcagctca aattctgttg cgttgtaatc tattcggcaa cgtactttgc cccgttgaga
    36481 agcctctggc acaactaaaa tatcagcaag gctaaaacga ggttcacagc caaaatattc
    36541 ttgtgttgct tgctcaaaac gttgttgatg atactgtaag ttctgaactt tgccgtcaat
    36601 aatcgaaagg gtttcaaata gcggaaatcg gcacatagac tttctctaac agttcattat
    36661 attcatcagt taacacactt tgtgaggtaa tgccaccacc actatgaaac acaagcccgt
    36721 ttgaggtttg agcaatgtaa cgaattgcca ccgcactttc taagctctcg ccatcaaaat
    36781 aaccaaaaat accggtgtag taaccccgtt ttcctaattc tgcttgctga ataatcgaaa
    36841 ccgttttctc tttcggtgca ccgctaatgg agccggcagg tagtaatttt gccaatatcg
    36901 aaccgatatt ttcttgccaa ttttcaccta attcgccaca gatctcggaa cttgtctgca
    36961 aaattgcccc tcgatgggtt tctacttttt ccacataacg gtatttagta acttcaatat
    37021 ttttcgccac caacgccaag tcatttcgga tcaaatccac aatggtatta tgttcggtaa
    37081 actctttttc cgaagccatt aatttctgct cggcattctc ctcatttgca ttaattgtgc
    37141 ctttcatcgg gtaggaaaaa atgcggttat tttcaattcg cacaaagcat tcaggggaaa
    37201 aacagacgaa cctgccttgc aaataacatt tatattttgc tttgctggcg gcaaaaattt
    37261 ccgccaatga ataattggtt tcaatggcag tcgggtagct caaattcagc aagtagctat
    37321 tgccggcttg aatttccttt ttcactaatt caaacccggt ttggtattcg ctaaacggaa
    37381 tcggatttgc cgataattca aaagctttag tcggtttttc aaccggcttt tcgagattcg
    37441 atttgcaaaa aaattcaaaa aaaagaccgc ttgcgtgagc ctgttctagc ggaaaaataa
    37501 gtggcttttg ctgctcaaaa tcaattaaaa agaaaaaagg gcgttttgct tgcccgtagt
    37561 gattggcttg gtgaataaaa tcttgcatag aaaacacaat ggctgaaatt ggctgaatca
    37621 tcaaaagctc tgttataatg caaagcaata gatttgaaaa gcatttttta aaaaaggttt
    37681 taccgatgaa caacaccatt cattttcaag aaattgggat ctacatcgcc cttgccgttt
    37741 gtgtgctggt aggcgtttat ctctttttct tgaaatcccg ttatcagcga aatgcctacg
    37801 aattaagcca agatcttggc aaaatcaccg aaaattttga acaaattcag caaaaatatg
    37861 aggtgttggc tcaagaaaaa aatcagcttg agcagtgggc aattcaacaa cagaccaaat
    37921 atgaagcagt gagtgaacgt ttaaacgagc gagatgggca attgcaacga ttccaacaaa
    37981 aaatcgaact tgccgaacag caagaaaacc agctggaacg ctatattaat gagctaaaag
    38041 aacgtgtggg ttcttcccaa gcaaaggcag aaagtttgga agaacagcta caatttagcc
    38101 aaagttcact ctcaactaaa gagcgggaaa atcaagcgtt atttagccgt ttaaacgagg
    38161 tacaaaacga gctgaccgaa ctgcgtacta cgctttcgga aaaacaggca aattttgaag
    38221 cacaacaacg caattttatt gaggtaaaac aacagcttaa tgtggaattt caacatttgg
    38281 cacaacaaat tcttgacgaa aaaagcaagc gttttagcga aaccaatcaa agctcgttag
    38341 aagctctact gaagccgttc aaagagcaaa ttgaaggctt tcaaaaacgg gtgaatgagg
    38401 tacatagtga gtcgctcaaa ggctcggcaa atttagaggc tgaaattaag cgggtgctac
    38461 aaattggcgt ttcaatgtcg gaagaggcac aaaatctggc aacggcatta aaaggcaata
    38521 ataaaattgc cggcaactgg ggtgaagtac agcttgaaag tgccttacaa tctgccggtt
    38581 tactcgcagg cgagcattac caagcccaag agagttttcg tgatgaagag gggcgacgtt
    38641 ttgcccccga ttttgtggtg catttgccgg atcaaaaaca tctgattatt gacagtaaag
    38701 tctcgttagt agcgtacgat caagcggtca gatcagaaga aaattttgca atttctcaag
    38761 ccttagacga acattgccgt tcgctacgca atcatattga gggattgtct aagaagaact
    38821 acagtgcatt attaggcatt aaaagcccgg attttgtact gatgtttatt ccgatagaac
    38881 cggcttatat tgaagcgatg aagcacgatc cgcaactgtt taactttggc tatgaacgca
    38941 atgtgatttt ggtttctcac accaccttaa tgccgatttt acgcacggtc gccaatttat
    39001 ggcggattga acgaggcaac agcgaggctc gtgaaatcag cgaaaaagcc ggcgaaattt
    39061 acaaccaagt gtgtgcggtt gccgatcggt tagctaaatt aggtaacacg ctaaataccg
    39121 caaataatca atacaatcaa accgttacct cgttagtggg acggcaaggc ttactgggca
    39181 aagtggaacg attccaacat ctttcaagca aagcaagcca aattgtgccg caggttgagc
    39241 tgattgaaaa tgaggtggat actattaaat tggaaatggt atcgagagaa ttattggaag
    39301 aatagtcaaa tctctttatt ccctacaaaa atatggggtg atttgaaata ctaaagaaaa
    39361 taataaaatg ctaaaaaaac tattaatcat cgacaaccac gactctttta ccttcaactt
    39421 ggtggattta ctccgtaagc ttgatgtgcc ggcgaaagtg gtgctggttg aagagcttga
    39481 cctcaatgag gtggagcatt ttagccatat tttgatttcg cccggccctg atgtgccgag
    39541 agcctatcta caaacctttg cgatgttgga gcgttatcat caaaccaaat caattctagg
    39601 cgtttgcttg gggcatcaaa cgatttgcga gttttttggc ggcaagttgt ataacctaaa
    39661 cgatgttaga cacgggcagc aacgcacgct cactcaaatt gagcctaacc ccatatttaa
    39721 aggtttgcct aagcagttga aagttgggtt gtatcactct tggggcattt tgcaaaattc
    39781 gttgcaaaat acaccgcttg taccaaccgc agtttgcgat caaaatgtgt taatggcgtt
    39841 taaacaccaa catttgccga tttttggcgt gcagtttcac ccggaatctt ttatcactga
    39901 atttggtcaa caaatgttgg caaattggct tgagattaca caagattttt agcgtgaaat
    39961 ccgctataat ccgtcagtta tttacccttt taaaatctgt aggggcgaaa aatttttcgc
    40021 cttacttaat atgcaagaga taatttaaat gaaagattcg attatcaata aattagaaag
    40081 tttaagtgaa cgccacgaag aactacaggc gttacttggc gatgcctcaa ttatttccga
    40141 tcaagacaaa ttccgtgctt attccaaaga atattcgcaa ttagaagaag tggttggcac
    40201 ctttaaccgc tggaaaaagc tgaacaatga tattgaagag gctcaattat tactcgatga
    40261 tcctgatatg aaggatatgg cagcggaaga aattgcggaa aataaagccg aaatcgagaa
    40321 tcttgagcaa catttacaaa ttttattatt gccgaaagat ccgaatgatg aatacaacgc
    40381 cttccttgaa atccgtgccg gaacgggcgg tgacgaggct ggtatttttg ccggtgattt
    40441 ataccgtatg tacagccgtt attgcgaaag taaacgctgg cgaattgaag aaatgtcggc
    40501 aaatgagagt gaacaaggcg gatataaaga gatcatcgtt aaaatttcag gcgaaggcgt
    40561 ttacggtcag cttaaatttg aatcgggcgg acaccgtgta caacgtgtac cgaaaaccga
    40621 atcacaaggg cgtattcaca cctctgcttg cacggtagcg gtaatgccgg agttgccgga
    40681 aagcgagatg ccggaaatca atccatctga tctgcgaatt gacacctacc gctcatcggg
    40741 tgcaggcggt cagcacgtta ataccaccga ctctgcagtg cggattactc atatcccgac
    40801 cgggattgtg gtcgagtgtc aagatgagcg ttcacagcac aaaaataaag ccaaagcgct
    40861 ggcagtgtta gcctctcgga ttgtacaagt tgagcaagaa cgccaagccg cagagcaagc
    40921 ggatacccgc cgtaacttac ttggctcagg cgaccgttcg gataaaatcc gcacctacaa
    40981 ctacccgcaa gggcgtgtga ctgatcaccg tattaacctc acggtttatc gtttagacga
    41041 agtgatgaac ggcaaaattg atgaattgat acaaccgatt atcactgaat atcaagcgga
    41101 tcagttagcg gcgttgtcgg agcaaaatta attttattgt aagggtacac tgtgtgtgcc
    41161 tgccaaatat aaatttgcaa aattttgact atttttgatc gcttgtagtt tctaaaaagt
    41221 ttaaaaattt gaggaaaaca aaatgagaaa gttaggttta ttagcagtat ttactttagt
    41281 attatcagcc tgttcaaatt caacaaaaat tgaacaaacc attctttcca agcaggatat
    41341 cgtaggctct tgggtatgcg ctattaaata cgatgatcta aaagtaagca ctttagattt
    41401 ttctgaattt aaagctaatg gcgaaatgac gaatttcggt gatatttctg atgagaattt
    41461 tgagcccatt aaatttactt atgtaacagg agataaaggg aagtgggact taaaaggaaa
    41521 tcgattagtg attgattatg atctttctga acgtaaagta aaaagaacaa caccaaaaaa
    41581 atttttagag ttcttgaaac aggaagagcg taaagcctca aaaacagcta aaaaactcct
    41641 taaatatgaa caagggattt tcaatattct gagtgatcag aaaaatactg aaaacagtaa
    41701 aataatatta gatattttga agtttggtca ttcaagaatg gcgattcagc aaaatatggg
    41761 agacaaagtt tattcaggtg gttgtgtaac agctgataaa gcagaagagt atttagatat
    41821 cctaaaaaat agaaattcta aataatggat taccgagagt ggctcacttt tgctgagcaa
    41881 aaattacttg aaaatagcgg ggcagatcca tacctaaacg caaaatttga tgcaaatttg
    41941 ctattgcaag cagtcacaaa acgttcaaaa tcggccattt ttgctttttc ggaaacccta
    42001 ctttctgaaa gcgagcttgc agaattagcg gaaaaattag ctcggcgtgc caaaggtgaa
    42061 ccaatggcat atattttagg ctatcgggaa ttttggtcgt tgccgttaaa ggtttcaccg
    42121 gcaacgctga ttcctcgccc tgatacggaa cgactggttg agttggcgtt ggaatatgct
    42181 aacaagcggt taggaaatca gaaaatgttg caaattctgg atctcggtac cggcacgggg
    42241 gcgattgcgt tggcactggc gagtgaatta ggcgagaaag cccaaattat cggggtggat
    42301 ttccaaaatg aggcggtgca actggcagaa gaaaatcgcc gaaacttagg cttaaatcaa
    42361 gttacatttt tacaaagcga ttggttcagt tcgcttgcaa atcaacaatt tgatttaatt
    42421 gtctcaaatc cgccttatat tgatgaatcg gacgaaaatt tgacggtggg agacgtacgt
    42481 tttgaacctt taaccgcatt agttgccgat aataaggggt tgagcgattt gcaaaaaatc
    42541 atcgaaaatg caccgcttca cttaaaaccg caaggggcgt taatgctgga acacggctgg
    42601 aaacaaggta acttggttcg agatttattt gatttacact tgtgggaaca ggtagaaact
    42661 gctcaagatt atgccggcaa tgaccgagta accaaagcga ttttaaaata aggcagaaaa
    42721 tggacgatat tattgaagaa ttgttaaaag agttggaaga tccgaaagta accatcagcg
    42781 aaaccgagct gaaaaagttt ctttatcgtg aaattttacg attaactacg ctcattgata
    42841 agaatgtaag tgagcaacaa gtgtttggtc agatgtctgc gttggtgaaa aaagctcgct
    42901 ataacgtgaa tggcgaaggc gatgaagaga gaattaacca attacttcat ttagtctatc
    42961 gtgaatgggg ctttcattgc tactatgaag actatttcca caccgaaaat ttgttgctaa
    43021 atcaagtaat tcgcaaacgg cgtggaatgc cggtttcact tggggcggtg gcgctttatt
    43081 tatccgccgt attggatttg ccgctttacc cggttaattt tccgactcag cttgtgttac
    43141 gggctgaaat acaccagccg aacggggcaa ccaaagtgcg ttttatcaac ccgtggaacg
    43201 gtgaatttct caccatcgaa atgctgaata aatggctgga aggagaaatg ggctttggcg
    43261 tagaagttac ccctgatttg ttaagacgag cagaaccaca agagttgctg gaaagggtcg
    43321 aaactgtgtt taaaatggca cttacccgtg aaggcaaata tgaagaaacc ttacgcttga
    43381 ttgaataccg cttggcattt agcccggaag atccgtacga aatcagagac cgaggaatgg
    43441 tactggcaag tatggattgc taccaagcag cattagaaga tattaattat ttcattgacc
    43501 aatgccctga agatccttct gccgaaattt taaaaatgga agtgaaagga ctgcaacaaa
    43561 aaagccaaga aagtgtggtg cattaagctg agaagtagcc ccaaaggggc tgagctatga
    43621 ttaacctagt acggctctgc cgtgctaggc agaaaataag aaacaaggac tccctatgac
    43681 agataaaatt gtaaaagttg gcaatattga agtggctaat cacaaaccgt ttacgctttt
    43741 tggtggtatg aatgtgctgg aaagccgtga tatggcactt agagtatgtg agcaatatgt
    43801 tgaagtcacg caaaaactaa atgtgcctta tgtgttcaaa gcctcatttg acaaagcaaa
    43861 ccgttcttca atccactctt accgtggacc gggaatggaa gaaggcttaa agattttcca
    43921 agaattaaaa caaacctttg gcgtgaacat cattaccgat gttcacgaaa tttaccaatg
    43981 taaacctgtg gcagaagtag ttgatgtgat tcaactacca gcatttttgg ctcgccaaac
    44041 cgatttagtg gaagcaatgg ctcgtaccgg tgcggttatt aacgttaaaa agccgcaatt
    44101 tttaagcccg ggtcaaatgg ggaatatcgt agaaaagatt gctgaatgcg gcaacgatca
    44161 agtgattttg tgcgaccgtg gcactaactt tggctacgat aatctagttg tggatatgct
    44221 tggtttcggc attatgaaaa aagtgtcgaa aggttgcccg gtgatttttg atgtgactca
    44281 ctcattacaa tgccgtgatc cattcggtgc ggcttccggt ggtcgcagag atcaagtaac
    44341 cgaactggca cgctccggta tggcgattgg tttagccggc ttgttcttag aagctcaccc
    44401 ggatccaaac tcggcgaaat gtgatggtcc atctgcatta ccgctgtcaa aattagaagc
    44461 ctttgtttca cagatgaaag cgattgatga gttggttaag agctttgaag aaattgatac
    44521 ttccaaataa actttctccg cattaaatcc tttcctactt tctttgcttc gccaaagaaa
    44581 gtaggcaaag aaaggcgacc ctacttcgct acttttcttc gcttagtgtg aatgggctta
    44641 acgaaaaatt tacattcgct tcgctcagag taaattttcc tacccattaa aacacgctca
    44701 ggtagctcag aagggagtta gaaaggtaaa agacagaata ggagaaaaaa atgctaaata
    44761 ttgtttttct tgaccgtacc ggtattcctg ccactcacga tattcctcgt ccgaattttc
    44821 cacatacttg gacggaatat gccaccacct ctgccgatca aacttttgag cgggtaaaag
    44881 aagcggatat tatcattacc agcaaagtgt tactccagcg tgacttgctg gctaaattac
    44941 caaagctgaa attaattgcg atcacagcaa ccggcacgaa caatgtggac ttagcggcag
    45001 caaaagagtt agggattgca gtgaaaaatg taaccggcta ttccagcgtt accgtgccgg
    45061 aacacgtcat tggtatgatt tactccctga aacatcgttt agccgattat caccgtgatt
    45121 taattacgtc tgaccgctgg gcaacttgcg gccaattttg ctatgtagat tacccgatcc
    45181 acgatgtgcg aggctcaacc ttaggcattt tcggtaaagg gaacatcggg gctgaagtgg
    45241 cacggctggc acaagcggtc ggaatgcagg ttatttttgc agaacatcaa ggcgcaactg
    45301 aaattcgaga aggctacacc gcctttgaag aggtattcaa acaagcagat attatttcac
    45361 tacactgccc tttaaccgag cagactcaaa acctgattaa tgcagaaacc ttagccttaa
    45421 tgaagccgac cgcttatatt atcaatacgg gtcgaggccc actgattgat gaacaggcat
    45481 tgcttgaggc attagagcaa ggcaagattg ccggcgctgc gttagatgtt ttagtgaaag
    45541 agccgcctga aaaagacaac cctttaatgt tagccgcaaa acgtttaccg aatttactga
    45601 ttaccccaca cgttgcgtgg gcaagcgatt ctgcggttac aacactagtg aataaagtga
    45661 cacaaaatat ggaagagttt gtagcaacgg gtatgtagct tgaagtaggg gctggtcctg
    45721 tgccagccct aatattaata gaatgagagg gcgggtataa aacccgcccc taccaatatc
    45781 attttagaaa aacagatgaa tttaacccct ttttttattg cttttcgcta ttttagatcg
    45841 aaaagtgctg accgtttcgg gcggttagtc accaatcttg ccagccttgg cattgtgctg
    45901 ggcgtaatgg cattggtgat tgtgctgtcg gttatgaacg gtttggaaaa tatgcagaaa
    45961 accaatctgc tatccacctt gccgcacgct atcatcaccc ctcaagaggg gcattttgcc
    46021 aaaggtgaaa aactggctct acctgaattt gccaagcaaa gtgtggtgat taaccgtgcg
    46081 aatgtgatta tccaaagttc acaaggcatt aacgccggtc agttaatcgg cgtggaaaat
    46141 gccacagacg atccgttgtt agtggcggaa aatatccccg cattattgcc tagcggtgaa
    46201 tttaaggtag taattggctc tcgtttagcc aataaattga acgttacggt aggcgataag
    46261 ctccgcttaa tgatcactga aaacagccaa tatacgccac ttgggcgagt gccggtacag
    46321 cgtttgtttg aaatttcggg cgtttatcaa tctaatcgtg aagcgagcga atttaccctc
    46381 tttgctaatt tagctgatgt cgggcgtttg cttcgcatca gcgaagatca agtacaaggc
    46441 gtgcgtttat atttgcaaga tcctttccaa gttaccgaac ttagccaata tttcgatgca
    46501 gaacgctacc aaatcagtga ttggcgtgag caaaaaggcg aatttttcca agcggtgaaa
    46561 atggaaaaaa atatgatggg cttactcatc agcctgatta ttgtggttgc aatttccaac
    46621 attgtgactt cactcagttt gatggtggta gacaaacagg gcgaaattgc aattttacaa
    46681 acccaaggct taaccaaaaa acaagtgatg cagatttttg tgttccaagg ggcgattgtc
    46741 ggagtaattg gctcgattat tggtggtttg ctcggcgtat tgatcgccta caatttagat
    46801 cagattatcc tgctattaaa cccgagcatc catctgccta ccctaatttc aggcacgcaa
    46861 attgcggtca ttgtaggggt gtcgatgttg ctttcactcc tttgcacgct ttacccggca
    46921 taccgagcct caaaaattga gccggcacag gcattgagat atgaataata agaacggaaa
    46981 taaaatgaca gaattacttc gctgtgaaca tatcagcaaa ttttacgatg agggcgaaca
    47041 gaaagttcag gtgctaaaag aggtgtcatt ttcgatgaac gaaggtgagc tggtagcgat
    47101 tgtcggtagt tcaggttccg gcaaaagcac cctattgcac acattaggcg ggctagatca
    47161 gccaagcacc ggtgaagtct ggattcgagg gcaatctctg caacagctca gttcagacaa
    47221 attagcgtta ttgcgtaacc aaaacttagg ctttgtgtac caatttcatc acttaatggc
    47281 agactttacc gcccttgaaa atgtgatgat gccgatgctg atcggcaaac agaacaaaac
    47341 tgaagctgcc aatcgtgccg agaaaatgct acaagcggtc ggattagctc atcgaatgac
    47401 acatcgccca tcagcacttt caggcggcga acgccaacgg gtggcgattg cccgagcgtt
    47461 ggttaataat ccggctctgg tacttgccga tgaaccgacc ggtaatttag accaaaaaac
    47521 cactgaaagc atttttgagc tgattcaaca gctcaaccaa gagcagaaca ttgcgttttt
    47581 attagtaacg catgatttaa atctagcgaa taaactttca cgtagtcttg taatgcgaga
    47641 tggcgtgtta agcgagggga gcttatgagt acgccgtttt tcattagctg gcgttaccaa
    47701 cggggcaaac agaaaaacaa actggtttca ctgattgctc tcttttccaa catcggtatt
    47761 gcccttgggg ttgcggtatt aattatcggg ctaagtgcaa tgaacggctt tgagcgagag
    47821 ctgaataatc gtgtgctttc ggttgtgcca cacgctgaac tggtttcgtt tcaaggcaat
    47881 cagcctcaac caatttttga tagcaaaaaa ttagaggcat tgataaaaaa atcgcaaaat
    47941 gtgaccgcta gttcgccttt tgtcagcttt accgccttaa ttgaaaatgg ggctcagctt
    48001 aaaatcgccc aggtgcgagg ggttgatcct aaaaagcaag atcaggccag ccaattaagc
    48061 caatttattc cacccgagca atggcaagcg ttcaatcagc aatttcaagc cgatgaagat
    48121 ggcttgattt taggagccgg tattgccaaa gcgttagaag ttgaggcagg agacgaagtt
    48181 acgcttttaa ttcctcagcc aacagaagac ggcaaattag cccagccctt gcgttttaac
    48241 ctaaacgtaa ccggcatttt gcggctagat gggcagctgg atcacagtta tgccttaatt
    48301 ccactgaata aagctcaaga gctgatggaa taccaagcga atgaagtgtc agggcttgaa
    48361 atcagccttg ccaatccgtt tgatgtacaa cagcttgaaa tgccacaact taacggctat
    48421 acccaaccgc tttatttgaa tacgtggatt gataaattcg gttatatgta taacgatatt
    48481 caactgattc gcaccgtgat gtacattgca atggttctcg tgattggcgt tgcctgtttt
    48541 aatattattt ccaccttgat tatggcagtg aaagacaaac aaggcgatat cgcgatttta
    48601 cggacactgg gggcaaataa cggttttatc cgccgcattt tcttatggta tggcttaatt
    48661 tcaggtatga aaggggcgtt attcgggctg attttaggtg taattttatc gctaaattta
    48721 accgctatta tcaaagcgat tgaagcattc tttgaggtga aattgctttc agacggggtt
    48781 tattttgttg atttcttacc aagtgaatta cattggcaag atgtcgtcta tgtattggca
    48841 gccacgatta tcctaagcct ttttgccagc ttataccctg ccaaccgagc cgctaaatta
    48901 gaaccggcaa aagtgttaag tgggcattaa tcattttcct attctaataa aaaagcagct
    48961 acccctcgat tatgtcagaa aggtagctgc ttttatttat gttattactc ttcgcctaat
    49021 aatttcaact cacgagcctt aatttggttt tttagaattt caactaagtc tgaaacgagg
    49081 taagtaccta agtctgcacc tttacgggta cgcacagaca ctttaccttc ggcgatttct
    49141 ttatcaccgc aaaccaacat ataaggcaca cggcgtaagg tgtgctcacg cactttgaag
    49201 ccgacttttt cgttgcgtaa gtctgctttc acacgaatgc ctgcatcaga aagctgtttc
    49261 accacttgtt gcacataatc tgcttggctg tcggtaatgt tcattaccac tgcttgggtt
    49321 ggggcaagcc aagttgggaa gaaaccggca tattcttcgg tgataatccc gatgaaacgt
    49381 tcgatcgagc ctaaaatcgc acggtgaatc attaccggtg tgcggcgacc attatcttct
    49441 gctacataag aggctgctaa acgacccggt agagcgaagt caagttgcac tgtgccacat
    49501 tgccattcac gtcctaagca atcacgcaat gcaaactcaa ttttcggccc gtagaatgca
    49561 ccttcgcctt cttgaatttc atattcaagt ccgttgttgc gtaaggcgtt ggctaagcct
    49621 ttttctgctc tgtcccacat tgcatcatca ccgatacggt tttccggacg ggttgaaagt
    49681 tttactgcaa tgttagtaaa accgaaggtg ctgtaaatgt cgtacaccat tttgatacag
    49741 ctgattactt ctgactcgat ttgttcttca gtacagaaaa tatgggcatc atcttgggta
    49801 aaaccacgca cacgcattaa gccgtgtaat gaaccggacg gctcgttacg gtgacacgaa
    49861 ccaaattccg ccatacggat cggtaaatca cggtaagatt tcaagccttg attaaagatt
    49921 tgaacgtgac ccgggcagtt catcggctta attgcatatt cacggttttc cgattgggta
    49981 gtgaacatta aatctgcgta gttttgccag tgtccggttt tttcccaaag cacacgatcc
    50041 atcataaacg gaccttttac ttcttggtaa tcgtactctt tcaatttggt acgcacaaaa
    50101 gtttcaagtt cacggaaaat cgtccagccg tcattgtgcc agaacaccat acccggtgct
    50161 tcttcttgca tatggtataa atctaacgct ttaccgatac ggcggtggtc acgttttgcc
    50221 gcttcttcca aacgttgtaa atattcggct aattgttttt tatcagccca agctgtaccg
    50281 taaatacgtt gtagcatttt atttttgctg tcgccacgcc agtaagcacc cgctactttt
    50341 tgtaatttga agtggtggca gaaacgcata tttggtacgt gtggaccacg gcacatatca
    50401 atatattctt cgtggtgata taacgcaggc gttgccgtac gttcaatatt ttcatctaaa
    50461 atcgccattt tgtatggctc accacgtttc tcaaaggtat ctcgagcttc ttgccagctt
    50521 accgggcgtt ttaccacgtc atagttggtt tttgccagct ctaacatacg cttttcaaga
    50581 gcgtctaaat cttcttgagt caaagagcgg tctaaatcga tatcgtagta gaaaccgttt
    50641 tcgatagtcg ggccgatagc cattttaacg tctgggaata attgcttgat ggagtgacct
    50701 aacaagtgag cggttgagtg gcgaatgatc tctaaaccat cttcgtcttt ggcagtaatg
    50761 atttcaagtg tgctgtcttg gctgatgata tcagacgcat cacggcgttc accgtttaca
    50821 cgaccggcaa tggtggcttt cgctaaacct gccccgatag attgggcaac ttccattaca
    50881 gaaactgggt tgtcaaatga acgttgtgaa ccgtccggta aagtaataat tggcataata
    50941 aatccttaca gtggtaatcc atacgagaga ttacttgtgt tgtttgaatg aaaaaaccgc
    51001 ctacaagcgg tcttttttga ttaaaatttt gcaatttgct tattatttcc cttacaagtg
    51061 gggaataaaa gcactgaaat tctagcataa aacaaagaaa aattaaactt tgctgttttc
    51121 agcggcccct tcatcaagct caatattttc attttctgtt tgtggatctt gttcaagtat
    51181 ttcaagctga gcctggtact cttgtaccgc ttcttcatca aatggaataa gtgcgtcctc
    51241 aacaacttca atttcttcaa gtgggcttgc ggttgcaatt ggggtagcat attgcatctc
    51301 tgctcgttcc gctttcggaa ttgatttggt gcggaaagca caaataaata aactaagtga
    51361 gaacagtcca aaaagtgcat aaaagccata ttcagcggaa aggctcatcg caaaaccgat
    51421 aagcggtgga gcaactaacg cccccacacc atagcaaaac agtaaacccc tgcttacttc
    51481 aaccgtgttc atattattcg gcaattcatc gttggcacgg gcaatgctca aagcatataa
    51541 gctaaacaga ctacagccaa agaaaaaggc gataatatac tgtccccagg cggtcaaatt
    51601 ttcttggaaa attgcaaata atccggcaag tgcggttaaa gtggcaatca ctgaacaagc
    51661 taaaatggcg ttacgtctgc caatacgatt cgataatatc gccatcggca tttggctgat
    51721 aaaaccaccg atcatcgaga taatcaaata aagcgagatt tcacttaatg aaaagctaaa
    51781 cttcagcaga aagactgacg ccattgtaaa aaagccgttt accaacattc cgctaataaa
    51841 gctggttgct aaggctaacg gtgaaatcgc aaatagttta ggaatactaa tgcgctctcg
    51901 agccggaata tcaggctgaa ccattttagt taaagcaatt gggatcatcg acatcacaac
    51961 caaaatggta gataagataa aaatgtttcg gctactaagt tctaaactta ataaacttac
    52021 gcctaaaata aaggcgagat agaagataat gttataagtg gcaagcactt tgcctctggt
    52081 atcgacattg gttttttcgc ttaaccaact ttcgactaaa agcagcaagc tataatgaca
    52141 gaatcccaaa ataattcgta acacgcccca gatccacaaa gtatcaatca taatatgacc
    52201 gagagcggcg atagcaaaaa cagcacagaa aacgctaaat gaacggatat gcccaacact
    52261 tgatacaatg cggtgagcaa aaatagtgct taatgccgcc ccaataaaga aagcagcgtt
    52321 aagtaaaccg atgatgatgt ccgttatccc catttctgcc agtttgatcc ctgctgaatt
    52381 tagaaataaa ccgtagccga aaaacagaaa aaagatacct gaataaaggg agtaaaattt
    52441 agtgctattc atcaatgctc cttcaaacat tctgccagta ttaaatcatt gtaccgattt
    52501 aatacctttt aggcaaaaaa gttattatta gcgttgagaa aataactttt ggctattttg
    52561 attaaaaaat caaatgaagg tgattttgaa atgtagtaaa aaatagagca atcagaattg
    52621 aaaaagggtt tgccgtaaca aagcctttag aaagattaga gtttcgagaa gaatttgtag
    52681 cttgggctaa ccaaaacact tgctaggagt gctttaagca caccacctaa aataaatgga
    52741 tataagcccg cttgtaatac tgtgccttct ggtacaaaga aagttgagcc aagccgaaag
    52801 caaaggtgat cacacttgca attaaggcga caaataaact tttccaaaaa gagtttaata
    52861 cgcctttatc gctataatag cctaagaaat aggcggttgg aatatagcct aataagtatc
    52921 cgcctgttgg accaaagaaa tgatggaaac ctgaagcacc gccggcaaag acaggtaaac
    52981 caatcgcccc ttcaaaaaga taaagtaata ctgcagcagt tgcggcttta cgacccattg
    53041 caaaaccaac cacagttaaa gcaagtgttt gccctgtaat gggtacaggg tacatcggca
    53101 cagaaatttg tgcaaaaata gcaagcagtg ttgctccaat aaaagttttc aaccaaaatg
    53161 tagttatact attggcttct agccaacgtt tagtttgaat taattgcgta gtgaacataa
    53221 tcctctcctt taatatggtt aaaatgttct caaccgatta tataagaaga tgtcttttag
    53281 ggtaagagtg aatttaaaat ggtaaaacca gtttatattt attgtgatga aggtagcagt
    53341 gaaattggtg ttcaatcact gttattggca gtaaaacagc atttacagcg tccggcttat
    53401 ctcatcaatg ccaaacaaat tattcaaagc aagttttccg atggctgttt attaatcatg
    53461 cccggtggag ccgatttacc ttactgcaaa aaattaaacg gagaaggcaa tcgtagaatc
    53521 cgccaattta ttgaacaagg tggagcttat ctcggtatct gtgccggagc ttattatggc
    53581 tgtgctgaat tgaattttgt agggcgtgac tatagcgtaa atggtaatcg agaactagct
    53641 ttatttaatg gcattgcggg cattgcgaaa ggcagtctgc cgcagtttac caacgggcaa
    53701 ctttacgata aaaacattgg tagcaaagcg atagttgaag taacacttgc agagggtgga
    53761 aaaatgccgt tttattatca cggtggttgc tgttttgaac caaattcaac aacagtgatt
    53821 tacaacccgt tggcttatta tcctgatggc tcagtagcaa tgatagaggg agagctaggg
    53881 cgaggttact atctgttgtc gggtgtacat tttgaattac aagcggtcgt ttattggcaa
    53941 aaaattgtaa attgctgtga aaatcttgac ataaaaacac aagaagaagc aatttgctct
    54001 aattttaatg taaattatgg ctcgcaaatt tggcaacatc taaagcaacg actaaatcaa
    54061 gcgtaaaaaa ataacctttc gctattctgc caaatggttt ctgcaatcag atttggtgat
    54121 tctgttctta attcacatag actattgaaa gtcactaaaa gccgttccgg tcggttcggc
    54181 tggccttgaa agccaaacac cggcatatcg ggcgagtcgg tttccagtag taaactttca
    54241 agaggtagtt tgcggatagc ctcacgggtt ttgttagcac gttcataggt aatagttccc
    54301 cccacaccaa ttttataacc gagatcgaca aaacgttttg cctgttcata gctgccggca
    54361 aagccgtgaa ccacaccggt tttggttagt tgggcttttt tgaggaacac aaaaagttga
    54421 tcgtggcttt tacgtgaatg aagattaacg ggtaaattaa attttttcgc aagggaaagt
    54481 tgtgcctcta aaaattcaca ttgctttttc cataattcag gggtagaaac ttcacttacc
    54541 gctttttcca aaccgatttc ggcaatagcg gtacactgtg gatcttgttg gcttagcaaa
    54601 ttttccaata agattaaatc ttgttgttgg tgctgttgaa tatacagtgg atgcaaccct
    54661 aatccataca ccaaattttc cggtttttgt ttggcacaag cggtcacttt tgcaaaattt
    54721 tttgcaaata cggcaacgat aagaatgcat tcaacctgat gctgtttggc attctcaacc
    54781 aattccggaa tgcttaaatt caggctttct gcgagatagt cgaggtgagt atgagtgtca
    54841 aaaaatcgca taataaaacc aataaaaacg gggactagat tgtccccatt tttagcattt
    54901 tacgcaagca attagtttgt tacataagca tcttcatcac gagcctcaga tggcttgagg
    54961 aaagcaaaga agcaaataac acaaactacc gtaatcgcaa aaccgatata aactgataat
    55021 tcataatcta aaccgaagcc gattttgttg ttcgcaaggt aagttgcaca cacggtagtg
    55081 ataaaccatg caggaatcga gcatacccag tggaattttt tatagcggta taagtaacct
    55141 gcggcagtcc ataacataac cattgcagtc atttggtttg cccaagtgaa gtaacgccat
    55201 aatacgctga aatcaacttt tgagacaatg aaaccaagta cgaataatgg tacggcaata
    55261 tacaaacgtt ttactaaggt acgttgttct aaaccaaaga tttccgctag ttgtaaacga
    55321 gcggctcgga atgcagtgtc acctgaggtg attggtaata ctacaacgcc taatacggca
    55381 aaaataccac cgataaaccc taagaagtgt aacgagctgt catataccac tttagacggt
    55441 gaaccggcag agatcgcatc ttgtaaggct tgtgggtttt catagaatga aagacctact
    55501 gcacaccata ctaatgcaat taccccttca gtaatcattg caccgtagaa aataaaacga
    55561 ccttctttct cattttgcgc acagcgagcc attaatgggg tttgggttgc gtggaaaccg
    55621 gataatgctc cgcaagaaat agttaagaat agtaatggcc aaattggaac atcacctttc
    55681 acttggaagt tttgggtgaa tttttcccac gttaagcctt gaccgtctgc attcactgta
    55741 cggaagaagt cgataggatc agtcgcactt aagtgagcgc tgactagacc atataccata
    55801 cctacagaca taaagagtaa taatgctccg aagaaaggat aaattcgacc gatgatttta
    55861 tcaacaggta gtaatgtagc aagaatatag taagcaaaaa tgattaacgt ccacacagaa
    55921 atcacggttg ctttatccat accccaaacg gttacgccac ctgatgctac agattcttta
    55981 attgcttcaa catcacctaa ttccattgca ctttgggttg cacctgcaat atccatggtg
    56041 atggtagaga gtaactgagc agggcttgca acgaaaacta cgcctactaa taaaagcaag
    56101 atgagcgcca ttacgttgat gaataacttg actggacgac ccaagaattt ccctgccaga
    56161 tttggcatcg ttgcgccacc gttgcggata cttaacatac cacagaagta gtcgtgtact
    56221 gcaccggcaa aaatacaacc aatcacaatc caaagcattg caactggtcc gtataaggca
    56281 ccaagtattg gtccaaaaat agggccggta ccggcaatgt ttaataattg aatcagccaa
    56341 atcttggttt tagacattgg catatagtca acaccgtcgt tcattgcata agccggcgtt
    56401 gctcttttcg ggttaatcac gaagattttt tcaatgattt ttccgtaagt aaagtagcca
    56461 agaacaagca cggctacaca gaaaaagaac cacaacatag tagaaatcct ataaattgag
    56521 ataaatgatc atttttagca gaactgataa aggcgtgcca ttgtaagtga ggagtgagga
    56581 taaagtaaaa tgaattgtta gatttgtgtg aataatttac tgaaaatgtg aggttcatca
    56641 aaaaatccct ttgttctgcg tcaatcaaag ggacttttta aagttaaata tcgaattttt
    56701 gaattttcgc ttcaatgcct tgggcatcta atccaagatc ggcataagct tcagcttgag
    56761 tagattgggg aacgaaaaag tcaggaatac caagcattac aagcggtttg attttgccga
    56821 tattttgcaa atattcattc acaaaactac ctgcgccacc ttgaatagca ttttcttcaa
    56881 gcgtaactaa caactcgtgt gaatccgcca ctttttgaag taatgcttca tctaatggtt
    56941 taatgaaacg catatctacc aacgtgtagt tatgtttttc agccacaatc tttgcttcat
    57001 caagcaaagc accaaaattt aaaatcgcaa cttttttacc ttcacgaata atgtttcctt
    57061 tacctacttc aagtctttgc attggctcaa gttttacccc tttagcattg cctcgagggt
    57121 aacgaatagc acttggcgta ttcatcgtgt aagcggtata aagcattttt cgcatttcat
    57181 tttcatcgct cggtgccata atggtcatat tgggaataca acgcataaaa cttaaatcaa
    57241 atgcaccttg gtgagtttgc ccgtcagcgc cgacaattcc ggctcggtca atcgcaaaaa
    57301 tcaccggcag gttttgaata gcaacatcgt gaattaattg gtcataagct cgctgcaaga
    57361 agctggaata aattgcgact accggtttgt aacctgcgat tgctaagcct gccccgaaag
    57421 tcactgcgtg ttgttcggca atcgccacat caaaatattg ttcagggaaa cgtttggaaa
    57481 attctaccat tcccgagcct tcacgcattg ccggcgtaat gccgataatt ttcggatcac
    57541 gctctgccat ttcacatagc caatcgccaa aaatattgga ataagtaggt gctgcttttg
    57601 attttggtaa ctcggctgcg gtagggtcaa atttcggaac gccatggtag ccaatcggat
    57661 cattttccgc aggtgtgtag cctttgcctt ttttcgtgcg aatatgcaga aattgagggc
    57721 ctttcagctc acgcatattg ctcaaggttt tgactaactc gtcaatatta tgtccatcaa
    57781 tcgggccgat ataattaaag cctaactctt caaacaaagt gctttcaggg gagaaaatca
    57841 cccctttcat atgctcttcg ctttttttca taaagttttt gatggtaggc actttatcta
    57901 aaattttctt actgccgtca cgcactgaag tatagagcga accagaaaaa atgcgggcaa
    57961 ggtggttgtt taatgccccg acattttcag agattgacat ttcattatca tttaagataa
    58021 ccaacatatc ggtatgcaat gcacctgcgt ggtttaatgc ttcaaatgcc attccggcag
    58081 tgattgcacc atcaccgatt acacagacgg ttttacggcc ggcattttct ttttctgctg
    58141 ccaccgcaat gccaagtccg gcactgatcg aagtagaaga atgcccaacg cttaacacat
    58201 cgtaagggct ttcttcacgc caagggaagg ggtgaatacc atctttttgg cggatagtgt
    58261 gcatttgatc tcggcgccca gtaaggattt tatgtgggta agcttgatga ccaacgtccc
    58321 aaattaattg gtcgaaaggc gtttgataaa cataatgcaa agctaccgta agctctacta
    58381 cccctaagcc agaggctaaa tgtccgctag tttgactgac tgattccaat aaataggctc
    58441 gcagttcatc aactacggtt tgaagctggt ctttagaaag taaacgtaaa tcttccggtg
    58501 agttgatttc gcttaacaga ggatacttct tttccataat gttttattac ccacaaccat
    58561 caaataatag atgatattaa attaagcttt gagttcttcg gtcaaagtag gctgaatgct
    58621 atttaaaatt tctttaacca cacggccgtt accaacaacc agattacctg ttttcaagta
    58681 atctgttccg ccaacaaagt tagtcacaat tgcaccggct tcacgggcaa taagttcacc
    58741 ggcggcgaaa tcccacggtt ttaagccgat ttcaaagaaa ccatcaacac gacccgctgc
    58801 aacataggca agatctaaag cggcagaacc ggtacggcgg aaatcagcta ctccgttatt
    58861 cattaagcct tcaagcatat ttaaatgtgc atcacgatgt ctgatgactt tgaaaggaaa
    58921 accggttgca attacagtgc cttttaaatc acggcgttct gcatcaatgc gtaaacggaa
    58981 ctcgtttaat ttagcccctt cgccacgcac cgcagtgaaa agctcgttac ggataggatc
    59041 gtaaactacc cctactgttg tacgtccgtt ttcacgcacg gcaatcgaca cagcaaaatg
    59101 aggtaagcgt tttacgaaat tggttgtacc atctaatgga tcgatcaccc attgtacatt
    59161 ttcattgcta ccgtttaaca cgccggactc ttcggtcacg atcgagtgtt cagggtaaga
    59221 tttacggatc acatcaataa ttgccgcttc cgctgcttta tcgatttcag tcacataatc
    59281 attcgtattt ttttgtaaaa cttgtgtatc ttgaggcgat tgttcatagc ctttggcaat
    59341 aatattgcct gcttttcgtg ccgcacgaat agcaatgttt aacattgggt tcatatttcc
    59401 accagatttt aaagaacata ataaagcacg aattataaag aatgtagcga aataaagaaa
    59461 caacaagcgg tctaatctgt gaaaaatttt gcaaaaaaat ggctttttct aaccgcttgg
    59521 agtttggatt cgttataata cctcccattc aagccaccca tttaggtact aaaaatgcaa
    59581 tttaaagaaa ataaccaacc tcgttttaaa cacagagaag aacgccgttc agatgagtgt
    59641 tccccccgtt tttcagataa aggcaaaatg cgaggaaatg aaggtaaaag tgacagaccg
    59701 cataagctac attttaagcg tgacgaggct tattcacgct ctgagcagat ttatccagca
    59761 gaaggcaaaa aagccagtgg tgaaggtagt gtgcaaattt gggtaaaagg tgggaatagc
    59821 tcagttaaag agaaaaaaaa cggcccgctt tcaccgagag cgcctgagaa aattaagaaa
    59881 aatcgcttgg aagagatgaa agtctatggc gaaaatgcct gtatcagttt gttccaacaa
    59941 cgtcctgaat ctattgtacg tttatgggca acagtggagg gggcaaaaaa attaggcgat
    60001 ttaatgagct atttggctca gcacaaaaaa gcctatcacg ttattgaccg tgaagagatg
    60061 gagcgagtca ccggtactga acatcacggc gatatgtgct tattggtgaa aaaaacggct
    60121 tcatttgtgc tagagggcta tttacaagtg ccgaaaaaac aagattgctt ggtactgtta
    60181 gatggtgtaa acaatgctca gaatgtgggg ggaatcatca gaacgtgtgc tttttatggt
    60241 gtgaaaggca ttattactga aaatgtggat agcttaaact ccagtgctgc agctcgtgtc
    60301 gcagagggtg gtttggaatt tgtgcgagcg ttagaaacta aaaacaagca tattgctttg
    60361 gtacaactcc gccaagccgg ttatcaaatt gtccacctca cacgccataa gcaagcccct
    60421 tctttaacca aaattaagct ggcggaaaag gtggtttttg tattaagtga gattcctgca
    60481 agtgatgtgg cttacgcaga agacacaaca atacagcttt cctttaataa tccgttaaat
    60541 agtggtttaa atgtggcggt aaacgcaggt attctactta atcaatggta tcaaacccac
    60601 attctttaag gaattgaaat gaatcagcaa aaaccgtttg aagtgattgc gattgttggc
    60661 acacctcgcc acgacagtgc atttgaaaca catcttgccg tttataattg gttaaaagat
    60721 cgtgattatc aaattttagt ggaaacagag gttgccactc agttacagct taaggaaagc
    60781 tacagtttag atcagattgg gcagcgcgca gagttggtga ttgtgatagg tggcgatggt
    60841 aatatgcttg gcatcgcccg agctttggct aaataccgag tgccactgat aggcattaac
    60901 cgtggcaatc tgggtttttt aaccgatatc gcacctcaaa cggcttttga acagctttat
    60961 agttgcttag tcaaaaatga gtatgtgatt gaagagcgat tcctgttaga ggctttcatt
    61021 gagcgcaacg gtaaaattat tgccgcaaat aatgccttga atgaaattgt ggttcaccca
    61081 acccaagtgg ctcgaattat tgaatttgaa gtttatattg acggaaaatt cgctttttca
    61141 caacgtgcag atggcttgat tgtggcaacg cctacaggct caaccgccta ttcactttct
    61201 gctggtggcc cgattttaac cccgaatatg aatgcaatgg cattagtgcc gatgcaccct
    61261 cacactcttt cttctcgtcc tttggtggtt gatggcgata gtcaaatttc gcttcgtttt
    61321 gccaaatata atacccctga tttagaagtg atttgtgacg ggcaggtgaa tatgcacttt
    61381 acgcctgaag atagagtctt ggtgcgtaaa agcccggata aattacagct gctccattta
    61441 aaagattata gctactttac tgtattaggt tctaaattag gctgggcaag taagttgttt
    61501 taaacacaag cggtgttttt tctgattaaa tttgcaaatg caatatgaat tgatttcata
    61561 tacatttcaa aaatatatca tttttattca ataaaaattt gtggttcaat aagctatctg
    61621 aaaaataaag gggacaataa aatgacaaca aattttaaat gccaagtgca tactattgaa
    61681 tttaatgaag attaccaacc ggagcaaagc acccgtttaa ccaccaactt tgccaacctt
    61741 gcccgtggcg aaaaccgcaa acagaacctg caaaatgcga ttgcgatgat caataaccgt
    61801 tttaacgatt tagcgaactg ggataacccg aatgcggatc gttattcggt gaaattggat
    61861 attgtctcac tggatattga tgtggaaggc aaaggcgagt ttttccctgc gattgagtgg
    61921 ctgaaaacct atgttatcga ctacaaaacc ggcaaaacca ttgatggcat tatcggtaat
    61981 aacttctctt cttttgtgcg tgattacgat ttcagcgtat tgttgttaga tcacaataaa
    62041 gacaaaccgc aatttagcgt gccggaaggc tatggcgagt tacacggcaa gctgtttaag
    62101 catttattgg catcgcctga atataccgca aaatataaaa aaccaccggt gatctgttta
    62161 agcgtggcaa ccacccgtac ctacacaaga accactaacg ttcacccaat tttaggtgtg
    62221 gaatatctgc aagatagtta ttcattaact gatgcctatt ttgcgaaaat gggcttaaaa
    62281 gtacgttact ttatgccgcc aaaatccgtt gcaccctatg cgttctactt tgatgagaat
    62341 gcagatttac tcaatgatta cactaactta gagctaatca gcacgattgc cacaatggaa
    62401 gcgttccaaa aaatttaccg tccggaaatt tacaatgcca attcggtcgc agggcaggtc
    62461 tataaaggca gcctgactta ccaagattat tccgtcacca aagtggaata tgaccgtgaa
    62521 gaacgcaccc agttggcgat caaacagggt aaatacaccg aagagcattt tattaaacgt
    62581 taccaagcga ttttagaggc ttgggcgacg gaggaataaa cgtagggtgg gcgtaagccc
    62641 accataaaat gagatataac caatggtggg cttacgccca ccctactttt tataggaaaa
    62701 ttcaaaatga gcaacattct tctcccaacc tctaccgccg gcagcctacc aaaaccttcg
    62761 tggcttgccg agccggaaaa actgtggtca ccttggaaat ttgaaggcga ggaactactt
    62821 caagccaaac gtgatgccct ccttgtttcc cttgccgaac aaattgcggc agggattgat
    62881 attgttagtg atggcgaaca gacccgccag cactttgtga ccacctttat cgagcatttg
    62941 gaaggggtgg attttagcaa gcgtgaaatc gtgcgcattc gtaaccgtta cgatgccagc
    63001 gtgccgagcg tggtgggggc agtttcccgc ccgaaatcgg tgtttgtgga cgatgccaaa
    63061 ttcttgcgtg cgcataccga caaaccaatc aaatgggcat tacccggccc gatgacaatg
    63121 atcgacactc tctatgacgg tcattacaaa agccgtgaaa aattggcgtg ggaatttgcc
    63181 aaaattctca acgaagaagc caaagagttg gaagcggcag gggtggatat tattcaattt
    63241 gacgaaccgg catttaacgt cttcttcgat gagctgaacg actggggcgt ggcggcgtta
    63301 gagcgtgcgg cggaaggctt aaaatgcgaa acggcggtgc atatctgcta cggctacggc
    63361 attaaagcca ataccgactg gaaacagacc ctcggcaacg aatggcgtca atacgaagag
    63421 agtttcccga aactgcaaca atcaaaaatc gacattatct cgttagagtg ccaaaactcc
    63481 cgtgtgccaa tggacttaat cgaattagtg cgtggcaaaa aagtaatgct gggagcgatt
    63541 gatgtggcta ccaacattat cgaaacaccg gaacaagtgg cagatacgtt acgcaaagca
    63601 ttacaatttg tcgatgcgga taaactttat ccaagcacca actgcggaat ggcaccactt
    63661 tcacgccaag tcgcacaagg taaattacag gcactcagcc aaggggcagc gatattgcgt
    63721 gcggaattcg caaaataatt ttgcgatctg catcgaaaat atcaactttg ctttttgctc
    63781 gtttccagat tttcttccaa aataacagga aatgacggag gcactatgct aaatctattt
    63841 aaaaagccaa ttaagaaaag ctcctttgag gcttgggcaa aactaatgga cgatgtggca
    63901 aaagttgcaa ttttagcaat tcctgttatt ctctatggag aatattcact gctaatgaaa
    63961 tttacacact caattacctt atttgcagta gtttattttt tgttagcaac ggcaagattg
    64021 tttcgagaaa cagcagagga gatgtaaatg gacggattga caatcggatt atttcttttg
    64081 cttgcatttt catttgctgc ataccaatgg gcaaagcatt taaaagatca taatcattaa
    64141 ttcaatgaat aa
//
LOCUS       DANLXS010000002        41960 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.002, whole genome shotgun sequence.
ACCESSION   DANLXS010000002 DANLXS010000000
VERSION     DANLXS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 41960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41960
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.002"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            126..422
                     /locus_tag="PXD71_000071"
     CDS             126..422
                     /locus_tag="PXD71_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL6290241.1"
                     /translation="MTAITIPQFEEVTSKQFQSEFGRIVDIVKGKTPVAITQYGRPTM
                     VLFPYDQAVEARRLLAKQRLLEMLAERDKNPPAALAEITLEELDKMIDEERNAL"
     gene            412..828
                     /locus_tag="PXD71_000072"
     CDS             412..828
                     /locus_tag="PXD71_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HDL6290242.1"
                     /translation="MHSDYLVIDTNILISGLLFPKSNPNLAVKKALLGYTICVSQATF
                     DEFLEVSARPKFAKYFSIQEREEFIANLLDAVKFVEIKETITDCQDPKDNKFLEVAIS
                     AKAKYLVTGDKKDLLSMNPYRNVEIISVSEFLAETR"
     gene            828..2486
                     /locus_tag="PXD71_000073"
     CDS             828..2486
                     /locus_tag="PXD71_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HDL6290243.1"
                     /translation="MKRFLTLFLLLFSSFSLAETHQIGILAQRGEAYTRTHWQPWVDW
                     LNSQFPNERFELVPLGINEASRRAELDFLLTNQAQFFYLSNQNVRWLATLNSPFSANG
                     EQGAVGSSIWVRADSHYQQLSDLKNQTISAVDNDAFGGFLLGLYQFHQAGMRQNRDFS
                     VQFSGFPVENALALLAEKQVEAAIVPVCLLEELEKEGKFKRSDFRLILQNPQAQGCLA
                     STPLLPNWSLAAMENVPNELAVQLATRLLNSHNPDLPRWTLPFSSAQADHILRELYRH
                     PQQKSLWATVLDWVRLNKFGLLAVVLFILLNLVALRYQVYRKSKALQQAHCKMQQYQQ
                     ELTRADRLALLGEMTTGFAHELKQPLSAVRMYAEGLKSQNSDPYQQRILDKLIAQVDR
                     AVKTMQSIRDWAQNRPSGEPQAVILNQLITNVIEFVAVENRQNAQISLIADRTFRLNL
                     HATVLEQVLTNCLLNALQAGASEISVRLQAVENGLEIAIEDNGGGFSPAQLEFPFVPF
                     RTDKPHGLGLGLVLCQRLMQSLNGRIVLANGENGAKVVLLLPQI"
     gene            2572..3229
                     /locus_tag="PXD71_000074"
                     /pseudo
     CDS             2572..3229
                     /locus_tag="PXD71_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249115.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(3213..4151)
                     /gene="ttcA"
                     /locus_tag="PXD71_000075"
     CDS             complement(3213..4151)
                     /gene="ttcA"
                     /locus_tag="PXD71_000075"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDL6290244.1"
                     /translation="MTDDIQKSDKKITYNFNKLQKRLRRNVGNAIADFGMIEEGDRVM
                     VCLSGGKDSYTLLDILLNLQQSAPVNFSVVAVNLDQKQPGFPEEVLPRYLESIGVEYK
                     IVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLF
                     LNMFYGGKMKSMPPKLISDDGKQIVIRPLAYCKEKDIIKYAEAKQFPIIPCNLCGSQP
                     NLQRQVVKEMLNTWDRQYPGRLETMFSALQDIVPSHLCDPKLFDFKGIKHGQTIEGIE
                     GDTAFDDVKIEAKTFLYEDDDNNFAEAGMIQFKEVN"
     gene            complement(4214..4927)
                     /gene="ubiG"
                     /locus_tag="PXD71_000076"
     CDS             complement(4214..4927)
                     /gene="ubiG"
                     /locus_tag="PXD71_000076"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HDL6290245.1"
                     /translation="MQNVDQQEITKFENMAKTWWDPNGSFKPIHLLNPLRLAYILEKS
                     NGLFGKKVLDVGCGGGILSEAMAKQGAIVTGIDMTSQPLEVAKQHAKESGLEIDYQQT
                     TIENFLAKMTACNQEKFDVITCMEMLEHVPDPLSIIQSCKALLKPDGVLFFSTINRTF
                     KAYMLVIIGAEYVLKMLPKGTHEFEKFIKPAELLAWCDQADLRCQEMRGYHFNPLTEK
                     FWLNNDVSCNYIATLKPNN"
     gene            4992..5372
                     /locus_tag="PXD71_000077"
     CDS             4992..5372
                     /locus_tag="PXD71_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDL6290246.1"
                     /translation="MTRINLVPPTELCDQHLLAEHRELTRIPNAVAKGKFSLKGQPED
                     YKLGEGHVRFFFNKLTFLKKRYDALHEECLARGFKVQYFWAEHLPQDPSLWLDYQPTE
                     TALTVNRERIALRMPANPRFSERK"
     gene            5469..8165
                     /gene="gyrA"
                     /locus_tag="PXD71_000078"
     CDS             5469..8165
                     /gene="gyrA"
                     /locus_tag="PXD71_000078"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDL6290247.1"
                     /translation="MSELAKDIIPVSIEDELKTSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFSMDQNSNTYNKPHVKSARVVGDVIGKYHPHGDFAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDAPAAMRYTEVRMQKITQELLTDLDKETVDFSPNYDGKEMIPDVLP
                     TKIPALLVNGSSGIAVGMATNIPPHNLGEVLDGCLAYIENEQISVEELMRYIPGPDFP
                     TGAIINGRKGIEEAYRTGRGKVYVRAKASVETTDKGREQIVVTELPYQVNKAKLIEKI
                     AELVKEKKIEGISKIDDYSDKKGISIVIEVKRDAVGEVVLNHLYSLTQMQVTFGINMV
                     ALDNGQPKVLNLRQIIEAFVKHRREVVTRRTIFELRKARERAHILEGLAIALANIDPV
                     IELIRASKTAEEAREGLLARPWALGNVAPMLEAAGVGASRPEDLPEHLGVRDGEYYLS
                     EAQARAILELRLHRLTGLEHEKIVEEYKELLTEIGELIHILTSPERLMEVIREELERV
                     KTDFNDERRTEITAASGDINIEDLIAQEDVVVTLSHAGYVKYQPLSDYEAQRRGGKGK
                     SATKMKEDDFIEKLLVANTHDTILCFSSRGRLYQLKVYQLPQASRGARGTPIVNILPL
                     VKEENERITAILPIPNGEFSADKFIFMATASGVVKKVSLDAFSNVRASGLIALKLREG
                     DELIGVDITDGNSEIMLFSAQGRVVRFAETAVRAMGRTATGVRGIKLSTTEISSEEIE
                     EAEIIETDNEEAEDSSSVSLDKDRVVSLVIPRTEGDILTVTQNGYGKRTVISEYPVKS
                     RATKGVVSIKVNERNGKVVAAVQVEETDQIMLITDAGTLVRTRVAEVSLVGRNTQGVR
                     IIRTAEDEQVVSLERVCEPEDDENESLDALENAETAETSTEE"
     gene            8260..10224
                     /locus_tag="PXD71_000079"
     CDS             8260..10224
                     /locus_tag="PXD71_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6290248.1"
                     /translation="MALINLSNAYLGFGDHPLLDHTELHIEPGERVCLVGRNGAGKST
                     LMKILAGEVQLDDGKLVFEKDIVVTRLEQDPPRHIEETVFDYVAEGIAHLADLLKQYH
                     HISLQMQTDYSDELLAKLSAVQSQLEHANGWQFENRIQDTLKLLELAPDKRLCELSGG
                     WVRRAALARALVADPDVLLLDEPTNHLDIEAIAWLENLLLDFKGSIIFISHDRAFIRK
                     MATRIVDLDRGKLVSYPSNYDKYLEEKAEDLRVEALQNELFDKKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKALREERRNRREVQGTAKIQIDNTARSGKIVFEVENAGYEIGGRTL
                     LKNFSTTILRGDKIALIGANGIGKTTLIKLLLGEIQPTSGSVRCGTKLEVAYFDQYRV
                     DLDPEKTVMDNVADGKQDVEVNGVKRHVLGYLQDFLFPPKRAMTPVKALSGGERNCLL
                     LAKLLLKPNNLLILDEPTNDLDVETLELLEEMLTDYQGTLIIVSHDRQFVDNTVTECY
                     FFEGEGVVNKYVGGYFDAKQQQENYFAATADKRLNSSKNLQKIEEKAPLAEQEKQKAD
                     QEKTKAEPTKKTKLSYKEQRELDELPAKMEQLEADIEALQAEVNSAEFFAKDPSYTNA
                     QLQKLADTEAALEQAFERWEALENIKNGNI"
     gene            10306..11064
                     /gene="tcdA"
                     /locus_tag="PXD71_000080"
     CDS             10306..11064
                     /gene="tcdA"
                     /locus_tag="PXD71_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDL6290249.1"
                     /translation="MTERLDNYEQRFGGIGRLYTPEGLEKLRRSHICVIGIGGVGSWA
                     VEALARSGIGKITMIDMDDICVTNINRQIHAMSGTVAQLKTEAMKERIERINPECVVE
                     IIDDFLTQDNLADYLNRGYDYVIDAIDSLRVKAAMIAFCKRNKIKIITTGGAGGQTDP
                     TKIQITDLSKTVQDPLAARVRSLLRKEYGFSNKNKFGVECVSSTQQLIFPQMGEACEV
                     SATMNCANGFGAVTMVTATFGFFAVSRVIDKLLK"
     gene            11111..12478
                     /locus_tag="PXD71_000081"
     CDS             11111..12478
                     /locus_tag="PXD71_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HDL6290250.1"
                     /translation="MAIHRVNPKGSMEQLSHLEMELLAKNTQGNLHQLYRNCSLAVLN
                     SGVHTDDSRALLSQYPDFEIRLLTREKGVSLELHNPPETAFVDGKMILNIQYHLFAVL
                     RDIVFVNALKNAIRPLEETSLEALTTNTVFSILRNAKAIEMNTDPNLVVCWGGHSINE
                     TEYQYCRAVGQEFGLRELNIVTGCGAGVMEAPMKGAAIGHANQRYKNGRFIGITEPSI
                     IASEPPNPIVNELVIMPDIEKRLEAFVRLGHAIVVFPGGPGTLEELLYIIGIKLNPAN
                     HAQQLPLILTAPKESADYFYAIDQFIGETLGEEARSLYEIIIDDSVLVARKVKQAMIE
                     VKSSRYESGDAYNFNWSLKIEEDFITPFIPTHENMANLNLSLAQPKEQLAANLRRAFS
                     GIVAGNIKPETSDLIEKYGVFKLHGDRKLMEHIDKLLERFVVQHRMKIPTGEAYQPCY
                     EIVKG"
     gene            12498..14045
                     /locus_tag="PXD71_000082"
     CDS             12498..14045
                     /locus_tag="PXD71_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDL6290251.1"
                     /translation="MYLKHIEISGFRGINHLSLQLRPNMVLIGENAWGKSSLIDALSL
                     IFNLEQALYQFQFKDFHIQYTDTPNQVRDIQLVFTFCEEHQHDLAVQFPAYLAQAAFY
                     IDNHRCVSLAVTGKLHGEQISTDYQFVDQSLKPIQIEQAENAIRQFIYHHPVYRLRDA
                     RLNNRVHNELLLKGSSQLEDDFQRELNALSSLLKYYFLTSQSRYLIKTSMQDTALWWE
                     KVKSLCLRLKQQPELVESAQQHISTLFLFNPRSTVIEKPIILFEDLNAQLHPRMIAIF
                     WELLGFLPSQRITTTNSIELLSQVPLREICRLVRHHDHTQAYWLERHSLGKEDLRKLT
                     FHIHHNRGLALFAKAWILVEGETEVWILQELARLLDIHLEMEGIKIVEFAQCGLRPLI
                     KYARAMGIEWYVLTDGDDSGKRYADTVKSMEEDFAHRLTMLPQKDIEHFFYKAGLRNV
                     FIQLANWRSQEKYPVSYIIKRAIQRSSKPDLAIALANEMGQRGEHSIPTLFKQLFADV
                     LALINKV"
     gene            14268..15302
                     /gene="metN"
                     /locus_tag="PXD71_000083"
     CDS             14268..15302
                     /gene="metN"
                     /locus_tag="PXD71_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDL6290252.1"
                     /translation="MIQLKNISKQFEVKGKKITALDNVNLEVPRGTIYGVIGASGAGK
                     STLIRCVNLLERPTIGQVIVDGQDLTAMSDKELIHARRNIAMIFQHFNLLSSQTVFEN
                     VALSLRLSNTPKEQVEKKVNELLDLVGLTDRKDVYPANLSGGQKQRVAIARALASDPH
                     VLLCDEATSALDPATTQSILQLLKDINKRLGLTILLITHEMEVVKRICDRVAVIDKGQ
                     LIESGSVSEIFSNPKTELAQKFIQSTFHFELPQEYTEQLSSSPTEHSKPIIKFEFTGR
                     SVDAPLLSMASKKFGIDFSILMSQIDYAGGVKFGFVIAEVSGNPDSIAEAKFYLIDNN
                     VKVEVLGYVG"
     gene            15292..15969
                     /locus_tag="PXD71_000084"
     CDS             15292..15969
                     /locus_tag="PXD71_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6290253.1"
                     /translation="MWADFMQEMTPQMWSLVWESTFETVYMGFVATALAVVIGLPIGF
                     LAFLTGKGEILENPRLHQVLDVVINIGRSIPFIILLIILLPFTRLVVGTTLGTTAAIV
                     PLSVSAIPFFARLTSNALLEIPSGLTEAAKSMGATNWQVVTKFYLPESLPILINGITL
                     TLVALIGYSAMAGAVGGGGLGNLAISYGEHRNMVYVKWIATIIIVAIVMISQKLGDHL
                     AKRYDHR"
     gene            15992..16825
                     /locus_tag="PXD71_000085"
     CDS             15992..16825
                     /locus_tag="PXD71_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL6290254.1"
                     /translation="MSFKKILGVALVSALALTACKEEKKAESTAAPAAQAPAKIKVGV
                     MSGPEHTVAERAAQIAKEKYGLEVEFVLFNDYALPNTAVSKGDLDANAFQHKPYLDKD
                     SQSKGLNNLVIVGNTFVYPLAGYSKKVKNVSELAEGAVIAVPNDPSNLARALILLEKQ
                     GLIKLKDNTNLFSTSVDIIENPKNLKIKEVDTSIAAKALDDVDLAVVNNTYAGQVGLN
                     TQDHGVFVESKDSPYVNIIVARQDNKDAANVQNFIKSYQTEEVYQEAQKHFKDGVVKG
                     W"
     gene            16908..17738
                     /locus_tag="PXD71_000086"
     CDS             16908..17738
                     /locus_tag="PXD71_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL6290255.1"
                     /translation="MNFKKLLGVALVSALALTACKDEKAQAPATTAKTENKAPLKVGV
                     MTGPEAQMTEVAVKIAKEKYGLDVELVQFTEYTQPNAALHSKDLDANAFQTVPYLEQE
                     VKDRGYKLAIIGNTLVWPIAAYSKKIKNISELKDGATVAIPNNASNTARALLLLQAHG
                     LLKLKDPKNVFATENDIIENPKNIKIVQADTSLLTRMLDDVELAVINNTYAGQAGLSP
                     DKDGIIVESKDSPYVNLVVSREDNKDDPRLQTFVKSFQTEEVFQEALKLFNGGVVKGW
                     "
     gene            17790..18581
                     /gene="metQ"
                     /locus_tag="PXD71_000087"
     CDS             17790..18581
                     /gene="metQ"
                     /locus_tag="PXD71_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDL6290256.1"
                     /translation="MKIMKLAGAVAIFSLFLTACNDKAEKLKVGVISGPEHKVMEVAA
                     KIAKEKYNRDVELVVFTDYATPNAALDKGDLDLNAFQHKPYLDNQIQEKGYKLVPVGN
                     TFVYPIAAYSKKIKSLAELKDGDTIAVPNDPTNLARALILLEKQDLIKLRADAGLKAT
                     SVDIIENPRKLVIQEIEAPLLPRTLDDVAFSIINTTYAGQNGLTPTKDGIFVEDKDSP
                     YVNLIVARENNQHSEAVKDLVKAYQTEEVYNKANEEFKGAMIKGW"
     gene            complement(18626..19777)
                     /gene="metK"
                     /locus_tag="PXD71_000088"
     CDS             complement(18626..19777)
                     /gene="metK"
                     /locus_tag="PXD71_000088"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL6290257.1"
                     /translation="MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACET
                     YVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPD
                     INQGVDRADPLEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMEQQSKVRKSGKLDW
                     LRPDAKSQLTFAYENNKIIGIDAVVLSTQHAEHISQKELIEGVMEEIIKPVLPSEWLS
                     QNTKYFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGKVSNEILVKLIYQNF
                     DLRPYGLIKMLDLIKPIYRETAAYGHFGRKHFPWEKTDKAEALRAGAGL"
     gene            complement(19960..20802)
                     /locus_tag="PXD71_000089"
     CDS             complement(19960..20802)
                     /locus_tag="PXD71_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDL6290258.1"
                     /translation="MKVGLVLEGGGLRGIFTAGVLDVFLDEHIHIDGAIGVSAGALFG
                     VNLPSKQKGRALRYNKKYAKDKRYMGLHSLLTTGNIVNRDFAYYELPSKLDPFDQQAF
                     EDSGMDFWVTVTNVETGEPEYIKIDNVFEQMEAFRATSSMPIVSKIVEIEGKKYLDGG
                     VSDSIPVQKFIEMGYDKIIVVLTRPLEYRKKPSSMWLFKRFYKKYPKLVQRWENRYAE
                     YNQAVEQIIQLNEKQQIFVIRPSRTVKISRLETDVNKIQAMYDLGVEDAKNALAGLRA
                     YLAK"
     gene            20862..21179
                     /locus_tag="PXD71_000090"
     CDS             20862..21179
                     /locus_tag="PXD71_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL6290259.1"
                     /translation="MADAEFMDFLLDQLSPISGLRSKKMFGEYCLFFDNKIVAIISKD
                     YRIFVKANPQTLPLFLAENAEQFHYFAKGKVQKMHYWSIPELAIEEADELVKWIRLGL
                     QAV"
     gene            21270..21983
                     /locus_tag="PXD71_000091"
     CDS             21270..21983
                     /locus_tag="PXD71_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HDL6290260.1"
                     /translation="MNQKKLVSLIVVIVLAIISQIFSFFDKKEPSKAQEKSTQSSQSH
                     QVSTSSLGNYDVQMKNDRLKQHIVDTDYYTLALSWSPAFCQKQKRNNHGKIPSHLEYQ
                     CGGSADFGWVIHGLWPQSGKARSVDEHPRYCQGDLPMVAEQTIKQYLPESPGATLLQG
                     EWEKHGACAFNQADDYFAKQKELFNQLKLPDTEMSKLALFNWMRANNPHLQNVYLGGS
                     KDELYICYDKAFQPMDCPR"
     gene            21983..22477
                     /locus_tag="PXD71_000092"
     CDS             21983..22477
                     /locus_tag="PXD71_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDL6290261.1"
                     /translation="MADLRQLKMQVQRQLKRDLERANHYFNTTFTPPSISYAVRGVKA
                     GVAYLERNEVRFNPVLLQENEQAFIRQVVPHELAHILVYQHFGRVQSHGKEWKMMMET
                     VLGVPAEIYHCFDTQSVQQQFSYQCACQTHQLSIRRHNAVMRDKKSYICRLCKTQLHF
                     VEKS"
     gene            22582..23862
                     /locus_tag="PXD71_000093"
     CDS             22582..23862
                     /locus_tag="PXD71_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL6290262.1"
                     /translation="METIVIVGGGAGGLELATYLGNKLGRKQKAKVVLIDRNATHLWK
                     PLLHEVATGTIDEGTDALSYRAHAKNHSFEFQQGTLVAVDREKKTVSLAPIYNDQNQL
                     LVKERHISYDKLVIAIGSKSNDFGTKGVAENCIFLDGSEQAKDFQKRMMELFLRFSHS
                     ENKDVKIAIVGGGATGIELSAELYHVVKNLNSYGFGKLNRASLKVTLIEAGPRLIPAL
                     PEKVSVSAFHELKKAGVSVRLGTMITEARPDGLMTRLGEKIEADIMVWAAGVKAPEIT
                     REFGFETNRLNQIEIKDTLQTTVDESVFVIGDCASLIQDGKPIPPRAQAAHQMATRCG
                     KNIVALIEGKEMKPFRFNDKGSLLSFSKFGTVGNLMGNLVSGDMFIEGKIARLAYLSL
                     YRMHQVALHGCVKTGLIVLVGQINRFLRPTMKLH"
     gene            complement(23949..24986)
                     /locus_tag="PXD71_000094"
     CDS             complement(23949..24986)
                     /locus_tag="PXD71_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6290263.1"
                     /translation="MNVLKLISLKSILIASGIFLSAQAAQAEGRLTVYCSVQNNVCEE
                     MTKQFSHKYNVETKFIHGGTGTIFGKVKAEASNPQADIWYGGTIEPHFQAGELGLLEA
                     YRSPKQAEILPQFKSLVESEQGKFTSIVYMLVLGLGVNTEKLAKLGIEAPKKWEDLSD
                     PRLKGEVQLPDPRSSGTTYTFMTTLSTLWGEEKTFDYLKKLNNNVSQYVKSTLVTSNL
                     ARGESAVTVGFVHSYATEKEKGAPIEAIIPEGKVGYALGGVSIIKNARNLDNAKLFMD
                     WVLSKEAQEIPWKKHGVYQTPTNINAEVAPQSTKAEALDFVEIDYQKAGSSEEGKRLI
                     DKWLAEIKLAN"
     gene            25208..25906
                     /locus_tag="PXD71_000095"
     CDS             25208..25906
                     /locus_tag="PXD71_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL6290264.1"
                     /translation="MENSWKYAFKLITPIFMGYFAAGVAYGMLATNAGMPAWFTIVMS
                     FTVVSGTAQYAAIPFFVSGVGAVSVFLSTLLMSLRFSFYTLNMMEHKPISKAKSLVAI
                     AGLTDEGFAVLSSLPKEQKQALFFKIAVLCVAYWTFSTIVGVLLGDSVANYIPHLDFA
                     LPCLFAILAYEQYKNQKQWKPIVIALIGFLLARQITETSVLLVAILVAIVIVALLPQS
                     SSIKLEKGVENEHK"
     gene            25917..26219
                     /locus_tag="PXD71_000096"
     CDS             25917..26219
                     /locus_tag="PXD71_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL6290265.1"
                     /translation="MFAALVLGSQICRFLPEFLPKKVLSSPILQKLNKMLPLVIMLLL
                     LLTSLNFPKNGEGFNLILAQILALIGVLGSYVWLKNTFLSVALGIFGVNVFLSLLG"
     gene            complement(26340..27641)
                     /locus_tag="PXD71_000097"
     CDS             complement(26340..27641)
                     /locus_tag="PXD71_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL6290266.1"
                     /translation="MSLFKFEERGSNARQEIIAGLTTFLAMVYSVIVVPGMLSQAGFP
                     AESVFIATCLVSGLGSILIGLWANAPMAIGCAISLTAFTAFSLVLGQQVSIPVALGAI
                     FLMGVVFTLISATGIRTWILRNLPSSIAHGAGIGIGLFLLIIAATNVKLVVNSGMDIP
                     VKLGDFTSFPVLVSLIGLAAIIGLEKLKVKGSILWVIIAITIVGLIFDPNVKYTGFFK
                     MPSFGENSQFLNLDIMGALNTAILPVVFALVMTAIFDATGTIRAVAGQAGLLDKDGQI
                     INGDKALTSDSFGSILSGLFGTAPAAVYIESAAGTAVGGKTGLTAVVVGIGFLFMLFF
                     QPLAFLVPSYATAPALMYVGLLMLSNVSKLDFSDFVGAMSGLVCAVFIVLTANIVTGI
                     MLGFATLVIGRLISGEANKLNLGTVIIAIILVAFYAFDLAI"
     gene            complement(27774..28748)
                     /gene="secF"
                     /locus_tag="PXD71_000098"
     CDS             complement(27774..28748)
                     /gene="secF"
                     /locus_tag="PXD71_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDL6290267.1"
                     /translation="MAIENVQQDVAEIKLPYKLIPFMKYRYVGFIFSLIVTALCIFTI
                     ATKGFNWGLDFTGGTVIETQFSQPADLAKLRTELENSGYGNALVQTTGSQKDLMIRLA
                     ASAGDMSVGNKVMEMIHQKLDPNATIKSIEFVGPNVGEELTQGAIYATLATLAMLLIY
                     VGTRFEWRLAVGGIVALFHDVLVTIGIFSFLQIEIDLTFVAAILSVVGYSLNDSIVVF
                     DRVRENFSKVRRSSSIEIIDISLSQTLSRTLMTSVTTLFVVLALFYLGGPTLHSFSLA
                     LLIGIGFGTYSSIYIAIGVALQLGLKREHMVQPKVEKEGADQDAFIDY"
     gene            complement(28758..30599)
                     /gene="secD"
                     /locus_tag="PXD71_000099"
     CDS             complement(28758..30599)
                     /gene="secD"
                     /locus_tag="PXD71_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDL6290268.1"
                     /translation="MLNRFPLWKNLMVIFVIAIGALYALPNLYGDDPSVQISGTRGQQ
                     ATPETLSQVQSTLSAMNIQPKSAVLENGSILVRLESSEQQLPAKEKISEALGNNYSVA
                     LNLAPATPQWLSDIGGSPMKRGLDLLGGVRFLMEVDMNTALAKQQDTLQDSLRSDFRK
                     EKLQYQAVKKGENFATEVEFADNDTAEKAVRLVRLSHPTLDILVNDNKVTLTPSAQAL
                     SESRTMAIEQNLSILRKRVEELGVSEPTIQRQGADRIVVELPGVQDTARAKELLGATA
                     TLEFRLVNTEANVASAAKGIVPADSGLQNTRDGEPVVLRRKPSLGGEHIIDATSGTDE
                     RGLPQVSIRLDTEGGEMMGEITKMAIQKPMATLYTEFKDSGRKDANGKVILEKHQEVI
                     NVATIQTRLGNQFQITGINSPAEAQNLAVLLRSGALIAPIVIVEERTIGASLGADNVK
                     QGMEASILGLGLTILFCLAFYKVFGIFASLALIVNMILTIGLMSLIGATLTMPGIAGI
                     VLAVGMSVDANVLIYERIKEEIRNGRPIQQAIHEGYNGAFSSIFDSNLTTILTSLVLY
                     GVGTGPVKGFAVTLALGVIISMFTAITGTRMLVNWVYGGKRVKKLWI"
     gene            complement(30639..30932)
                     /gene="yajC"
                     /locus_tag="PXD71_000100"
     CDS             complement(30639..30932)
                     /gene="yajC"
                     /locus_tag="PXD71_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL6290269.1"
                     /translation="MQQGGGMEMIFILIIFGAIFYFMIYRPQAKRQKQQRDLLASLTK
                     GDEVLTSGGLIGKITKVTADNDNIVIALNDTTEVTIKRDFVVAVLPKGSLKSL"
     gene            31133..31690
                     /locus_tag="PXD71_000101"
     CDS             31133..31690
                     /locus_tag="PXD71_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDL6290270.1"
                     /translation="MKKLFAQAVIFGAFFASSAFAHNVQLNQALPAVSVAKDGELAVA
                     GGKVSYKNWHSSSLAGKVRVIHHFAGRSSVKEKNEDLMTAIKNAGFDRSKYQTVTIVN
                     ADDAIVGTGVFVKNSVENGKLDNPHSQVVLDQQGSVKKAWGLKEKESFIAVLDKSGKV
                     QFTSEGKLSAKQIQEVIGLVKTLTQ"
     gene            complement(31731..32699)
                     /locus_tag="PXD71_000102"
     CDS             complement(31731..32699)
                     /locus_tag="PXD71_000102"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDL6290271.1"
                     /translation="MKLFTLENAFLRITLSDVGASWLSCIVKMPNEEREVLVTTSAKN
                     WQKQTAFFGATIGRYANRIANAEYRLNGKTYQLAKNNGEHNLHGGELGADKIVWQLES
                     QSEQAVKFSYFFADGEEGFGGEVKAFVEYRLTENELQISFDAVANQDTPLCLTNHAYF
                     NLQGSETILDHELIINADGYLPVDASGIPNAPLKKVESTSFDFRNAKKIGQDLLADDD
                     QKAVKGYDHSFHLAKNSENPTACLAVEDLKMELRTSMPALQLYTGNWLKGQPNLTKGE
                     YEDYAGVALEPEFFPNSLNQPELLQFGGITKAGEEYHHTISYRFII"
     gene            complement(32825..33988)
                     /gene="galK"
                     /locus_tag="PXD71_000103"
     CDS             complement(32825..33988)
                     /gene="galK"
                     /locus_tag="PXD71_000103"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL6290272.1"
                     /translation="MKPQEIALQQFEQHFGYKAERTVFAPGRVNIIGEHTDYNDGFVM
                     PCAINYGTAVSFAKRSDNKWRVFAIDLNQQDEFDLSQAIEPSEQKWANYVRGVVKFIQ
                     AKHPEFKQGADLAITSDVPMSSGLSSSAALEVSIGKTAQLLGDLPLSLPQIALIGQEA
                     ENKFVGCNCGNMDQLTSALGQQDHLVMIDCRSLEITPTPVPQGYSIAIINSNVKHDLV
                     TGEYNSRREQCEFAAHFFGKAKLRDVTAEQFIERASELSEENELAYKRAKHIISENQR
                     VLEAVEALKANDMVKLGELMAQSHDSMRDDFEITCPEVDCLAEIAQQAIGKNGGARMT
                     GGGFGGCVVCLVPNDKVEDLRQAVANNYEKQTGIKETFHVCTASDGVRECPAR"
     gene            complement(33998..35044)
                     /gene="galT"
                     /locus_tag="PXD71_000104"
     CDS             complement(33998..35044)
                     /gene="galT"
                     /locus_tag="PXD71_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDL6290273.1"
                     /translation="MSEFVLNDHPHRRFNPLKNQWILVSPHRAKRPWQGQQEEIVQDE
                     KPSYDPSCYLCPGNKRITGEQNPNYTKPFVFKNDFSALLPDTPAPIPNDDPLFQISHT
                     QGESRVICFSPDHSKTLPQLSIEEITDVVTVWQEQANELKEHYQWVQIFENKGSMMGC
                     SNPHPHGQIWASNFLPNEIAIEDECQAKYFAQYGSPLLLDYAQRELDKQERIVAETED
                     WIAVVPYWAAWPFETLLLPKAKQFKSITDLNEAEKADLALALKKLTTRYDNLFNISFP
                     YSMGFHFAPFNGKENAHWQLHAHFYPPLLRSATVRKFMVGYEMMAESQRDLTPEQAAE
                     RLNAVSDTKHYKDK"
     gene            35257..36108
                     /locus_tag="PXD71_000105"
     CDS             35257..36108
                     /locus_tag="PXD71_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HDL6290274.1"
                     /translation="MERLNKFDAWLMNHKPNARLSGASRLLTEFWFFGLKEARACLFA
                     GLFFIAMFSVPKTGLWGLPRYDILLIFAIVVQIAMVKLGLETWDELKAISLFHLVGFV
                     LEVFKTSSNIQSWAYPDFAYTKLFNVPLFTGFMYAAVGSYIIQAWRLFDLKIKSHLPY
                     WLATLTALMIYLNFFTHHYIGDYRWYIAAFALGLYARTFVFFTPYDITRKMPLLLSFF
                     LIGFFIWLAENIGTFAGIWRYPNQFGAWSMVHMSKWSAWALLVMMTFTIIVHLKHIKQ
                     TVRVSSD"
     gene            complement(36194..37279)
                     /locus_tag="PXD71_000106"
     CDS             complement(36194..37279)
                     /locus_tag="PXD71_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL6290275.1"
                     /translation="MEYRYYITTALVIAVLQLFLFNFAKTVSWLFNLSSKNRKTVGIL
                     TFLVANGLVILHLTRTFALFRIMAFILVLLLFAFFISFFTACIYKIFNGNTTVNRWLK
                     IAYPFAFFGLLGLGYYNAYTPTVVHYSVKLDKPLEKPLRIGMASDLHLGKLFGTKQLD
                     QLAEIFNQQKVDLILLPGDIMDDNTEVYVADKMQPHLAKLKAPLGVYATLGNHDFFGA
                     QETIAKEITDAGITVLWDQTAEINGKFTLIGRNDDLVKNRPSAEQLLQNVNTKLPVIL
                     LDHRPTEIEQHAKLPIDIQVSGHAHKGQVFPASIITKLIYRLHYGYEKIGFGHYFVTS
                     GYGFWGVPMRLGSQSEVMIIDVEGENP"
     gene            complement(37301..38281)
                     /locus_tag="PXD71_000107"
     CDS             complement(37301..38281)
                     /locus_tag="PXD71_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL6290276.1"
                     /translation="MLFDSIQFSIGVTLPTILLMLFGIFPRRRKFIDDTFCNMASKVV
                     FNFTLPPMIFLNMLKSPLDFSSQINLISTGVGGSILIYLWAEWWAARHIENRRYRCIF
                     TQGTFRTNAAILGIALILNAYGDKALAAASVYISCLVITFNVLAVITLTSSLSEKKVN
                     FGRLILSILRNPLILAVIAGLTFNYFELSHQIPTPLESAGKLMASLTLPLALICTGAS
                     INFKQLKQFNQGVESTINKIVLFSASIRLIFAPIFLLLLGKFVFQLPPMELGIVFVAA
                     SAPVASATYAMTRNYGGDGEAAANLIGITTLGSMFSASIGLFLLRQIGWV"
     gene            complement(38384..39482)
                     /gene="prfB"
                     /locus_tag="PXD71_000108"
     CDS             complement(join(38384..39406,39408..39482))
                     /gene="prfB"
                     /locus_tag="PXD71_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550889.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL6290277.1"
                     /translation="MFELNPIKNQLTDLSERTAVIRGYLDFEHKVERLEEVNAELEQP
                     DVWNKPEKAQALGKERAMLEQIVNTIKALEQGIEDVEGLIELAVEAEDQDTFNEAQVE
                     ADELEKKLADLEFKRMFSGPHDSADCYLDLQAGSGGTEAQDWTEMLLRMYLRWAESKG
                     FKAELMEASDGDVAGIKSATVKISGDYAYGWFRTETGIHRLVRKSPFDANNGRHTSFS
                     AAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQN
                     GRSQHQNKDQAMKQLKAKLYELEMSKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DHRTGVEHRTTQQVLDGDLDKFVEASLKAGL"
     gene            39738..40646
                     /locus_tag="PXD71_000109"
     CDS             39738..40646
                     /locus_tag="PXD71_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDL6290278.1"
                     /translation="MAKRPSDIDATLLQLEILRRIPKLPAKIDAKSLHQQLLDAGFER
                     DIRTVQRSLKMLCEHFDIECDERSKPFGYSWKIRSEGLALPILNEQQSLLLKLAEQQL
                     KYLLPANLMASMKPFFEQADRMVSGAKDKPEYQWLNKVCSTPTSLPLIPAKIKEEIFN
                     SVSQALFQNKLLKIEYQNQHGKKHQATIMPLAISQQGASIYLVCRYEGFDDNRLLALH
                     RIRKAEISTFSFERPKDFNLQAYQDAGHLGFGNGEKASLTFSISRDAGFHLTETPLAK
                     DQKILEESETHYRIQAIVADNDMLTW"
     gene            40947..41588
                     /locus_tag="PXD71_000110"
     CDS             40947..41588
                     /locus_tag="PXD71_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDL6290279.1"
                     /translation="MPFQLTFCQQAGNQHQKNQDALFNGKAVYQWQLKNAESYLLDND
                     KVIFGVADGVSSHPRNHLASHYLMALLSQCQSLDSQWLRQAHGDFCEKLAKDYFGASC
                     TFVACELHHNGRGRMVNVGDSRAYKITANGEWQQLSFDHTILAEMKQKGLVKEGSNYA
                     SMYNGLTDCIVADFDADDFRIHIAEFQLEKGESILLCSDGLHEQLAPEAIQAI"
     gene            41755..>41960
                     /locus_tag="PXD71_000111"
     CDS             41755..>41960
                     /locus_tag="PXD71_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDL6290280.1"
                     /translation="MKNDFHYAAELIRNADGILITAGAGMSVDSGLPDFRSVGGLWNS
                     YPPLKKFNLQFMQIATPLAYQERP"
BASE COUNT        12164 a         8528 c         9369 g        11899 t
ORIGIN      
        1 gtttgttcaa gtttttatgt tattgaattt acaataacta caaaaattta tcttaattgg
       61 ctcgattgat ttttacacaa aacatagcta acatatccaa aatcactcac attaggagct
      121 taaaaatgac agcgattact atccctcaat ttgaagaggt gacttctaag caatttcaat
      181 ctgagttcgg cagaattgtt gatatcgtaa aaggcaaaac ccctgtggcg attacccaat
      241 atggcagacc gacaatggtg ctttttcctt acgatcaagc ggttgaagct cggcgattat
      301 tagcaaaaca gcgtttgctt gaaatgcttg cagagcgaga taagaatccg ccggcagctc
      361 ttgcagaaat cacgcttgaa gagttggata aaatgattga tgaggaacgt aatgcactct
      421 gattatttag tgattgatac gaatattttg attagtggtt tattgttccc aaaatcaaat
      481 cccaatttag cagtgaaaaa agccttatta ggctatacca tttgtgtctc gcaagcgact
      541 tttgacgaat ttttagaagt ctccgctaga ccgaaatttg ctaaatattt cagtattcag
      601 gaaagggaag aatttattgc caatttgttg gatgcagtaa agtttgtgga aattaaagaa
      661 acgattacag attgccaaga tcccaaagat aataaatttt tagaagtggc gatcagtgca
      721 aaagcgaagt atttagtgac gggcgataaa aaagatctcc tcagtatgaa cccttatcgg
      781 aatgttgaaa ttatttccgt cagcgaattt ttagcggaaa ctcgctaatg aaacggtttc
      841 tcactctttt tcttctcctt ttttcatctt tttcattagc cgaaacccac caaatcggca
      901 ttctcgctca gcgtggggaa gcctacaccc gcacccattg gcagccttgg gtggattggt
      961 taaattcgca gtttcccaac gagcggtttg agctggttcc attggggatt aacgaggcga
     1021 gccgccgggc ggagttggat tttctgctga ccaatcaagc tcagtttttc tatttgagta
     1081 accaaaatgt gcgatggctt gccacgctca acagtccgtt ttcggcaaat ggtgagcaag
     1141 gggcggtggg cagtagcatt tgggtgcgtg ccgacagcca ttatcagcag ttaagcgatc
     1201 tgaaaaatca gactatcagt gcggtggata acgatgcttt cggcggtttt ttgcttgggt
     1261 tgtatcagtt tcatcaagca ggaatgcggc aaaatcggga tttttcggtg cagtttagcg
     1321 gttttccggt ggaaaatgcg ttggctttgc tggcggaaaa acaggtggaa gcggcgattg
     1381 taccggtctg tttgctggaa gagttggaga aagagggcaa gttcaagcgg tcggattttc
     1441 gcttaatttt gcaaaaccca caggcgcagg gttgccttgc cagcacgccg ttgttgccga
     1501 actggtcgct ggcggcaatg gaaaatgtgc caaacgagct ggcggtacag cttgccacac
     1561 gcttgctaaa cagccacaat cccgacctac cccgctggac gttgccgttt tcttccgccc
     1621 aagccgatca cattctgcgt gaactgtatc gccacccgca acaaaaaagc ctgtgggcaa
     1681 cggtgctgga ttgggttcgt ctcaataaat tcggcttgtt ggcggtggtg ttgttcattt
     1741 tgctcaacct tgtcgccctg cgttatcagg tctatcgcaa aagcaaggcg ttacagcagg
     1801 cacactgcaa aatgcagcag tatcagcagg aactgacccg agccgaccgc cttgctttgt
     1861 tgggcgagat gacaaccggt tttgctcacg aactgaaaca gccgttgtcg gcggtgcgaa
     1921 tgtacgccga ggggctgaaa agccaaaaca gcgatcctta tcaacagcgg attttggata
     1981 agctgattgc gcaggtagat cgggcagtga aaacgatgca gagcattcgg gactgggcgc
     2041 aaaaccgccc gagtggtgaa ccgcaagcgg tcattttgaa ccaattgatt actaatgtga
     2101 tcgagtttgt ggcggtggaa aatcgccaga atgcacaaat ctcgctgatt gccgaccgca
     2161 cttttcggct taatttacac gccacggtac tggaacaagt actgacgaac tgcctgctca
     2221 acgccctgca agccggtgca agcgagatat cggtgcggtt acaagcggtc gaaaacgggc
     2281 tggaaattgc aatcgaagac aacggcggcg gtttttcccc tgcccagctt gaatttccgt
     2341 ttgtgccatt tcgcaccgac aaaccacacg gcttagggct aggcttggtg ctatgccaac
     2401 ggctgatgca atcgctcaac ggacggattg tattggcgaa cggggagaac ggggcgaagg
     2461 tagtgctttt actccctcaa atataaatgt aggggcaggt cctgtgcctg cccgtaaatc
     2521 ctatcaaatt cagggcgggc acaggacccg cccctacgga aaaaagaaaa aatgattatt
     2581 cacattcttg atgacgaaga aagcatttta gacgctatgt cgttcctgct tgccccgctt
     2641 ggggtggaaa tccaaacgtg gcaatccagc ctagattttt tggcacaggc ggatttacac
     2701 cggcaaggcg tgctgttact agatattcga atgccgctgc cagacgggca acaggttcat
     2761 caacaactgc gagaggcaca atcgaccctg gcggtggtga ttatgaccgc ccacggtgat
     2821 gtgccgatgg cagtcgctga attgaaaaaa ggcgcagtgg attttctgca aaagccggca
     2881 agttttgaac agctaaaaca ggcgattgca caggcggaaa aagtaagtga aaaagcggtg
     2941 aaaattcgtg aaaattcgtg aaatttcgca aaattacgcc aaattgacgg aaaaagagcg
     3001 taaccttgtg ccactgatta tgcaaggttt caccaataaa cagatcgcag ataaattagc
     3061 gatttcagtg cgtacggtgg aagtacatcg agccaatgtg atggaaaaaa tgcaggcgga
     3121 gagtttggcg gggctggtgc agatgatcgg ggagttacaa tataaaggta gcggaaattg
     3181 ctctgctacc tttagtttcg acttttgccg tattaattta cttctttaaa ctgaatcatt
     3241 cccgcttcgg caaaattgtt atcgtcatct tcgtataaga aggttttggc ttcaattttg
     3301 acatcatcaa aagcggtgtc gccttctatg ccttcaatgg tttgaccgtg tttaatccct
     3361 ttaaaatcaa acaatttcgg atcgcataag tgggatggca cgatatcttg caaagcactg
     3421 aacattgttt ctaaacgccc cgggtattgg cgatcccaag tattcagcat ttctttcacc
     3481 acttggcgtt gtaaattagg ctgagaacca cacagattac acggaataat cgggaactgc
     3541 ttggcttcgg catatttgat aatgtctttt tctttacaat aggcgagtgg gcggatcaca
     3601 atttgcttgc cgtcatcaga gatcagttta ggtggcatac ttttcatttt gccgccgtag
     3661 aacatattta agaataaagt cgccagcata tcgtcacggt gatgccctaa tgcaattttg
     3721 gttgcaccca attcggtggc ggtgcggtat aaaatcccac ggcgaagtcg tgagcaaagt
     3781 gaacaggtgg ttttgccctc cggaattttc tcttttacga tgccgtaggt gttttcttcc
     3841 acaattttgt attccacgcc aatgctttct aaatagcgag gcaatacctc ttccggaaat
     3901 cccggttgct tttggtctaa attgaccgct accacactaa aattcaccgg tgcactttgc
     3961 tgtaaattga gcaaaatatc taacagcgta tagctgtctt taccgccgga aaggcagacc
     4021 attacacggt cgccctcttc aatcatacca aaatcggcaa tggcattacc gacattacgg
     4081 cgtaaacgtt tttgtaactt gttgaaatta taagtaattt ttttatcaga tttttgaata
     4141 tcgtctgtca taatagttgt actaggctta gaaattagcg gtgtatagta aagatttcgc
     4201 actattttgc aagttaatta ttcggcttca gcgtagcgat atagttacaa ctcacatcat
     4261 tattgagcca aaatttttcg gttaatgggt tgaaatggta gcctctcatc tcttggcaac
     4321 gtaaatcagc ctgatcgcac caagctaaca attcggcagg cttgatgaat ttttcaaatt
     4381 cgtgcgtgcc ttttggcaac attttcagca catattccgc cccgataatt accaacatat
     4441 aggctttaaa agtacggttg atggtagaga aaaacagcac gccgtcaggt ttgagcaggg
     4501 ctttacagct ttgaatgata gaaagcggat cgggaacgtg ttccagcatt tccatacaag
     4561 taatcacatc gaatttttct tgattacaag cggtcatttt tgctaaaaaa ttttcaatcg
     4621 tggtttgttg gtaatcaatc tctaacccac tttctttggc gtgctgcttt gccacttcaa
     4681 gcggttgaga ggtcatatcg atgccggtaa caatcgctcc ctgttttgcc attgcctcac
     4741 ttaaaatacc gccaccacag ccgacatcta acaccttttt gccaaataaa ccgttcgatt
     4801 tttctaaaat ataggctaaa cgcagtggat taagcagatg aatcggcttg aaactgccgt
     4861 ttggatccca ccaggtttta gccatatttt caaattttgt gatttcttgc tgatcgacgt
     4921 tttgcataaa ctgttcctat ggtaaattta gggcattact ataacgcatt tagcctaaaa
     4981 ggaaaatcag aatgacccga attaaccttg ttccgccaac ggaattatgc gatcaacatc
     5041 tgcttgccga acatcgtgaa cttactcgga ttccgaatgc ggtagcgaaa ggcaaattta
     5101 gcctaaaagg acagccagaa gattacaaac tgggcgaggg acacgttcgg tttttcttca
     5161 ataaactcac gtttttgaaa aagcgttacg atgcgttaca cgaggaatgt ttagcaagag
     5221 gttttaaggt gcaatatttc tgggcggaac atttaccaca agatccctct ctctggctag
     5281 attatcagcc gactgaaacc gctctgactg taaatcggga gcgaattgca ttaagaatgc
     5341 cggcaaatcc acgttttagc gaaagaaaat gaccgcttgt acgccttttt gttgcttttt
     5401 atgctataat ctcgccaatt ttttcgcctt atttaaggct cttttactaa taaaattagg
     5461 aaatttcaat gagcgaatta gccaaagata ttattcccgt aagtattgag gacgaattaa
     5521 aaacctctta ccttgactat gcgatgtcgg tgattgttgg gcgtgccttg cctgatgtac
     5581 gagacggttt aaagccggta caccgccgag tgcttttttc aatggatcaa aacagcaaca
     5641 cctataataa gccgcacgtt aaatctgccc gtgtggtcgg tgatgtgatc ggtaaatatc
     5701 acccacacgg tgactttgcg gtgtataaca ctattgtgcg tatggctcaa ccgttctcac
     5761 tacgttatat gttggtggac gggcaaggta actttggttc tattgatggc gatgcaccgg
     5821 cagcaatgcg ttataccgaa gtgcgtatgc agaaaattac gcaggaatta ttaaccgatc
     5881 tcgataaaga aacggtggat ttctcaccga actatgacgg caaagagatg attcctgatg
     5941 tattgccaac caaaattccg gctctgttag tcaatggttc ttcagggatt gcggtcggta
     6001 tggcaaccaa tattccacct cataatttag gcgaagtatt agatggttgc ttggcgtata
     6061 ttgaaaatga acagattagt gttgaagagc taatgcgtta tatcccgggg ccggatttcc
     6121 cgaccggggc gattatcaac ggtcgtaaag ggattgaaga ggcttaccgc accggtcgtg
     6181 gcaaggtgta tgtgcgcgcg aaagccagcg tggaaacaac tgataaaggg cgtgagcaaa
     6241 ttgttgtaac cgaactgcca taccaagtga ataaggcaaa attaattgaa aaaattgccg
     6301 agctggttaa agagaagaaa atcgaaggca tcagcaaaat tgatgactat tccgataaaa
     6361 aagggatttc gattgttatt gaagtcaagc gtgatgcagt gggtgaagtg gtgttaaacc
     6421 acctttattc tttaacccaa atgcaggtca cgtttggtat caatatggtg gcgttagata
     6481 atgggcagcc aaaagtgctg aacttacgcc agatcatcga agcctttgtt aaacaccgtc
     6541 gtgaagtggt cacacgccgt acgatttttg aattgcgtaa agcccgtgaa cgtgcccata
     6601 ttttagaagg tttggcgatt gcgttagcga atatcgaccc ggtaattgaa ttaattcgag
     6661 cctcaaaaac cgcagaagaa gcccgtgaag gcttattggc tcgcccttgg gcattaggca
     6721 atgttgcacc aatgttagag gctgctggtg ttggtgcttc ccgaccggaa gatttacctg
     6781 aacacttagg agtgcgtgat ggcgaatact atttatctga agctcaagcc agagcgattt
     6841 tagagctacg cttacaccgc ttaaccggtt tagaacacga gaaaatcgtt gaagaataca
     6901 aagaactctt aactgaaatt ggcgaattaa tccacatttt aaccagccca gaacgcttaa
     6961 tggaagtgat ccgtgaagag ttagaacgtg taaaaaccga ttttaatgat gaacgccgta
     7021 ctgaaattac cgcagcctct ggcgacatca acattgaaga cttaatcgca caagaagatg
     7081 tggtggttac gctttctcac gccggctatg tgaaatatca gccgttatcc gactacgaag
     7141 cacaacgccg tggcggtaaa ggtaagtcgg caaccaagat gaaagaagat gatttcatcg
     7201 aaaaactctt ggtggcaaac acccacgaca ccattctttg cttctcaagc cgtgggcgtt
     7261 tatatcaatt aaaagtgtat caattaccgc aagcaagccg tggggcaaga ggcacgccga
     7321 ttgtgaatat tctgccgtta gtgaaagaag aaaacgaacg tattaccgcg attctaccga
     7381 ttccaaacgg tgaatttagt gccgataagt ttatctttat ggcaacagct agcggtgtag
     7441 tgaaaaaagt ttcccttgat gcctttagca acgtgcgtgc aagcggtctg attgcgttaa
     7501 aacttcgtga aggcgatgag ctgatcgggg tagatattac tgatggtaac agcgaaatta
     7561 tgttgttctc ggcacaaggg cgagtagttc gctttgccga aacagcagtt cgtgcgatgg
     7621 gacgtaccgc caccggcgta cgaggtatta aactttctac caccgaaatt tcaagcgaag
     7681 aaattgaaga agctgaaatt attgaaactg acaatgaaga ggcagaagat tcctccagcg
     7741 tcagccttga taaagaccgt gtggtttctt tagtgattcc tcgcaccgaa ggcgatattt
     7801 taaccgtcac ccaaaacggc tacggtaaac gtacggtgat tagcgaatat ccggtgaaat
     7861 cccgagcaac caaaggggtc gtttcgatta aagtgaatga acgaaacggc aaagtggttg
     7921 cggcagttca agtagaggaa accgaccaaa ttatgttgat taccgatgcc ggcacgctgg
     7981 ttcgcacccg agtggcagaa gtcagcctcg ttggacgaaa cacccaaggc gtacgtatta
     8041 ttcgcaccgc agaagacgaa caagttgtta gccttgaacg agtctgcgag cctgaagacg
     8101 atgaaaatga aagcctagac gcattagaaa atgccgaaac agcagaaaca tcaactgaag
     8161 aataatctta actaaacaag cggtcgaatt ttcacaattt tttgcaaaaa tagaaggaaa
     8221 ttcgaccgtt tttggatcat acagtagata ataaaaacta tggcacttat caatctttcc
     8281 aacgcctatt taggctttgg cgatcatcct ttattggatc atactgaatt acatatcgaa
     8341 cccggcgagc gggtttgttt ggtggggcgt aacggggcgg gtaaatcgac cctgatgaaa
     8401 attctggcgg gcgaagttca gcttgatgac ggcaagttgg tatttgaaaa agacattgtc
     8461 gttacacgtc ttgaacaaga tccgccccgc catattgaag aaacggtgtt cgattatgtg
     8521 gcggagggga ttgcccatct tgccgactta ttgaagcaat atcaccacat ttccctgcaa
     8581 atgcagaccg attacagcga tgaactgctc gccaaacttt cagcggtgca atcgcaatta
     8641 gaacacgcta acggttggca gtttgaaaac cgcattcaag acaccttgaa actgctggaa
     8701 ctcgcccccg acaaacgcct gtgcgaactc tcaggcggtt gggtacgccg tgcggcattg
     8761 gctcgggcgt tggttgccga tcctgatgta ttgctgttgg acgaaccgac caaccacctc
     8821 gacattgagg cgattgcgtg gctggaaaat ctgttattgg atttcaaagg cagtattatt
     8881 tttatctcgc acgaccgtgc ctttatccgc aaaatggcga ctcggattgt ggatttagat
     8941 cgaggcaaat tggtttccta cccaagcaat tacgataaat acttggaaga aaaagccgag
     9001 gatctgcgag tagaagcgtt gcaaaatgag ctgttcgaca aaaaactcgc ccaagaagaa
     9061 gtctggatcc gtcaagggat taaggcaaga cgcacccgaa acgaagggcg tgtgcgagcg
     9121 ttgaaagcgt tgcgtgaaga gcgtagaaac cgccgagagg tacaaggcac ggcgaaaatt
     9181 cagatcgaca acaccgcacg cagcggcaaa attgtgtttg aagtggaaaa tgccggctat
     9241 gaaatcggcg gcagaaccct gctgaaaaat ttcagcacca ccattttgcg tggcgataaa
     9301 attgccctga ttggagcgaa cgggatcggc aaaaccacgc tgatcaagct gttacttggc
     9361 gaaattcagc cgacatctgg ttcagtgcgt tgtggcacaa aattggaagt ggcgtacttc
     9421 gaccaatacc gagtcgatct tgaccctgaa aaaaccgtga tggacaacgt ggcagacggc
     9481 aagcaagatg tagaagtaaa cggggtgaaa cgccacgttc ttggctatct gcaagacttc
     9541 ctgttcccgc caaaacgggc gatgacaccg gtgaaagccc tctcgggtgg tgaacgcaac
     9601 tgcttgttgc tggcaaaact gttactcaaa ccgaacaact tgctgatttt ggacgaaccg
     9661 accaacgatt tggacgtgga aaccctcgaa ctgctggaag agatgctcac cgactatcaa
     9721 ggcaccctga ttattgtgag ccacgaccgt caatttgtgg ataataccgt aaccgaatgc
     9781 tacttcttcg agggcgaagg cgtggtgaat aaatatgtcg gcggctattt cgatgccaaa
     9841 caacagcagg aaaattactt cgccgccact gccgacaagc ggttaaattc gagtaaaaat
     9901 ttgcaaaaaa tagaggaaaa agcaccgctt gcggagcaag agaaacagaa agccgatcag
     9961 gaaaaaacga aagcagagcc gacgaagaaa accaaacttt cctacaaaga acagcgtgaa
    10021 ctggacgagt taccggctaa aatggaacag cttgaagccg acattgaagc cctgcaagcc
    10081 gaagtaaata gtgccgaatt cttcgccaaa gatccaagct acaccaacgc ccagctacaa
    10141 aaacttgccg ataccgaagc cgcgttagag caagcctttg agcggtggga agcgttggaa
    10201 aatattaaaa acgggaatat ctaatgtagg ggcgaatgat tattcgccct tgatggataa
    10261 aatgattggg cgaaaaattt ttcgccccta caaatgtgaa gcaaaatgac agaaagatta
    10321 gataactacg aacaacgttt tggtggcatt ggtcgccttt atacgccgga aggattggag
    10381 aaactacgcc gttcgcatat ttgcgtgatt gggattggcg gggttggctc gtgggcagtg
    10441 gaggcattgg ctcgctcagg tatcggtaag atcacgatga tcgatatgga cgatatttgc
    10501 gtgaccaata tcaaccgcca aattcacgca atgagtggca cggtggctca actgaaaacc
    10561 gaagcgatga aagagcggat tgagcggatc aaccctgaat gtgtggtgga aattattgat
    10621 gatttcttaa cgcaggataa tctcgcagac tacctcaatc gaggatatga ctatgtgatc
    10681 gatgcgattg acagtttgcg ggtaaaagcg gcgatgattg cgttttgtaa acgcaataag
    10741 atcaaaatta tcaccaccgg cggggcgggc gggcagaccg atccgaccaa aattcaaatt
    10801 accgatttaa gcaaaacggt gcaagatccg ttggcggcaa gggttcgtag cctgttgcgt
    10861 aaagagtacg gtttcagcaa taaaaacaaa ttcggcgtgg agtgcgtttc ttcgactcag
    10921 caactgattt tcccacaaat gggtgaggcc tgtgaggtgt cggcaacaat gaactgtgcc
    10981 aacggctttg gagcggtgac aatggtgacg gcaacgttcg gcttttttgc ggtgagtcgg
    11041 gtgattgata aactattaaa ataacaagcg gtcatattaa atgaattttt tgcaaatcaa
    11101 ggagtaaata atggcaatac atcgagtcaa cccgaaaggg agtatggaac agctttccca
    11161 tttggaaatg gagttgttgg caaagaatac gcagggtaat ttgcatcaac tttatcgtaa
    11221 ttgctctttg gcggtgctaa attccggtgt gcataccgat gacagccgag cgttgttaag
    11281 ccaataccct gattttgaaa tccgcttatt aacccgtgaa aaaggcgtgt cgcttgaatt
    11341 gcataatcca cccgaaactg cgtttgttga cggcaagatg attttgaata ttcaatatca
    11401 cctttttgcg gtgctgcggg atattgtgtt tgtgaatgcg ttaaaaaatg cgattagacc
    11461 gcttgaagag acttctttag aagccttaac cacgaatacc gtattttcga ttttgcggaa
    11521 tgcgaaagcg attgaaatga ataccgaccc aaatttggtg gtgtgctggg gagggcattc
    11581 gattaatgaa accgaatacc aatattgccg tgccgtcggg caagagtttg gcttgcgtga
    11641 gctgaatatt gtgaccggtt gcggagccgg tgtgatggaa gccccgatga aaggagcggc
    11701 tatcggacac gccaatcaac gttataaaaa cgggcgtttt atcggcatta ccgagccgtc
    11761 tattattgcc tccgagccgc caaacccgat tgtgaatgaa ttggtaatta tgccggatat
    11821 tgaaaaacgg ttggaagcct ttgtgcgttt agggcacgcc attgtggttt tcccgggcgg
    11881 gccgggaacg ctggaagagt tgctgtatat tatcggtatt aagttaaacc cggctaacca
    11941 tgctcagcaa ttaccactga ttttaaccgc cccgaaagaa tcggcagact atttctatgc
    12001 catcgatcaa tttattggcg aaactttagg cgaagaagct cgcagtttgt atgaaattat
    12061 tattgatgac tcggtgttgg tggctcgtaa agtcaagcaa gcgatgattg aggtaaaaag
    12121 cagccgttat gaaagtggcg atgcttataa tttcaactgg tcgttaaaaa ttgaggaaga
    12181 ttttattacg ccatttattc caacccacga aaatatggca aacctgaatt taagtttagc
    12241 acagccaaaa gagcagctgg ctgcgaattt aagacgggca ttttcaggga ttgttgcagg
    12301 taatattaag ccggagacct ccgatttaat tgagaaatat ggggtgttta aattacacgg
    12361 cgatcgtaaa ttaatggagc atatagataa actgttggag cgttttgtgg ttcaacaccg
    12421 tatgaaaatt ccaacgggtg aggcgtacca accttgctat gaaattgtaa aagggtaatg
    12481 ctaacaaggg gtaaggtatg tatttaaagc atattgaaat cagtggcttt cgtggtatta
    12541 accatttatc gctccagcta cgaccaaata tggtattgat tggcgaaaat gcgtggggga
    12601 aatccagtct gattgatgca ttaagcctga tttttaacct tgagcaggcg ttgtatcaat
    12661 ttcagttcaa agatttccat attcaataca ccgatacccc caaccaagtg cgggacattc
    12721 aactggtgtt taccttttgt gaagagcatc agcacgattt agcggtgcaa tttcccgctt
    12781 atttggcaca agccgcgttt tatattgata atcaccgctg tgtttcgctt gccgtaaccg
    12841 gaaaattaca cggagagcag attagcaccg actatcaatt tgtggatcaa agcctaaaac
    12901 cgatccagat agaacaagcg gaaaacgcca ttcggcagtt tatttaccat caccctgttt
    12961 atcgcctgcg tgatgcaagg ctcaataaca gggtgcataa tgaattatta cttaaaggat
    13021 cgtctcaact tgaagacgat tttcaacgtg aattaaacgc cctctcttcg ctactgaaat
    13081 actattttct aaccagccaa agtcgctatt tgatcaaaac cagtatgcaa gacaccgcat
    13141 tgtggtggga aaaagtaaaa tccctctgtt tacgcctaaa acagcagccg gaattggtcg
    13201 aatctgctca gcagcatatt tctactctat ttttgtttaa tcctagatct acagtaatag
    13261 aaaagccgat tattttattt gaagatctga atgctcaact acaccctcga atgattgcga
    13321 ttttttggga actgctcggt tttttaccca gccaacggat caccactact aattctattg
    13381 aattgttatc gcaagtgcct ctgcgtgaaa tctgccgttt ggtgcgtcat cacgatcata
    13441 cccaagccta ttggcttgaa cgccacagtt taggcaagga agatttgcga aaattaacct
    13501 ttcatattca ccataatcgt ggcttggcac tgtttgctaa agcgtggatt ttggttgagg
    13561 gggaaacgga ggtttggatt ttgcaagagc tggcgagatt attagatatt catttggaaa
    13621 tggaagggat caaaatcgtt gaatttgctc agtgtggctt acgtccgtta attaaatacg
    13681 ccagagcaat ggggatcgaa tggtatgtgc tgactgacgg tgacgattcc ggcaaacgtt
    13741 atgcagatac ggtaaaaagt atggaagagg attttgctca ccggctcacg atgttgccac
    13801 aaaaagatat cgaacatttt ttctacaaag caggcttgcg gaatgtgttt atacagcttg
    13861 caaattggcg ttcacaagaa aaatatccgg tgtcctacat cattaaacga gcgattcaac
    13921 gaagctcaaa gcctgattta gccattgcat tagccaatga gatggggcaa cggggagagc
    13981 attcaattcc aactttattt aaacaattat ttgcagatgt gctggcattg ataaacaaag
    14041 tatagaatac aagcggttaa atttgacaaa aaattgccaa ttttgaccgc ttgtttgctt
    14101 gtgatgaaaa ccaatcatac aaaactctaa tttatttcaa gtgcaagcta ttattttatg
    14161 cgttatgctt atttggaata aaaaaatcgg gttagttgat tgacatagca ttctagacgt
    14221 ctaaaataac caaaaataat agttaattga atgtggataa atagaaaatg attcagctga
    14281 aaaatatcag caaacaattt gaggtaaagg gcaaaaaaat taccgctctg gataatgtaa
    14341 acctagaagt accaagaggc actatttatg gtgtcattgg ggcatcaggg gcaggaaaaa
    14401 gtaccttaat tcgctgtgta aatttattag aacgcccaac aatcgggcaa gtgattgtcg
    14461 atgggcaaga tttaacggca atgtcggata aagagttgat tcacgctcgc cgtaatattg
    14521 caatgatttt ccaacatttt aatttgcttt catcacaaac tgtatttgaa aacgtggcgt
    14581 tatcgttgcg tttaagcaat acgccaaaag agcaagttga gaagaaagtc aatgagctgt
    14641 tggatttagt ggggttaaca gatcgtaaag atgtttaccc tgcaaatctt tccggtgggc
    14701 agaaacagcg tgtagcgatt gctcgtgcgt tagccagcga tccacacgtt ttgttgtgtg
    14761 atgaagcgac cagtgcgtta gatccggcga ctacgcaatc cattttgcag ctattaaaag
    14821 atattaataa acgtcttggt ttgacgattt tattaatcac tcacgaaatg gaagtggtaa
    14881 aacgcatttg tgaccgagta gcagtgattg ataaagggca gttgattgaa tctggctcgg
    14941 taagtgaaat tttctcgaat ccgaaaaccg aattagctca gaaatttatt caatcgactt
    15001 tccattttga attaccgcaa gaatataccg agcagctttc aagttcgccg actgaacata
    15061 gtaagccgat tattaaattt gagtttaccg gacgttcggt ggatgcacct ttgctttcaa
    15121 tggcatcgaa gaaattcggc attgatttta gtattttgat gtcgcaaatt gattatgcag
    15181 gcggggtcaa atttggattt gtaattgcag aagtgagtgg taatccggat tccattgcag
    15241 aggcaaaatt ttatttaatt gataacaatg taaaagtaga ggtgttaggc tatgtgggct
    15301 gattttatgc aggagatgac gccacagatg tggagcttag tatgggaatc tacttttgaa
    15361 accgtttata tgggctttgt ggcaacggca ttggcggtag tcatcggttt accgattggt
    15421 ttcttggcgt ttttaaccgg taaaggcgaa attttagaaa atccgagatt acaccaagtg
    15481 ttagatgtcg ttattaacat cgggcgttcg attccgttca ttattttatt gattattttg
    15541 ttgccattta ctcgcttagt tgtgggcaca acattaggca caacggcagc gattgttcct
    15601 ttaagtgttt cggccattcc gttttttgcc cgtttaactt caaatgcgtt gcttgaaatt
    15661 ccgtccggtt taacagaagc ggcaaaatca atgggagcaa caaattggca ggtagtgaca
    15721 aaattttatt taccggagtc attaccgatt ttgattaatg gcattacgct aacgctggtt
    15781 gctttaattg gctactcagc aatggcgggg gctgttggtg gcggcggttt aggcaattta
    15841 gcgattagct atggcgaaca ccgtaatatg gtgtatgtga aatggattgc taccattatt
    15901 attgttgcga ttgtgatgat tagccaaaaa ttgggtgatc atttagcaaa acgttacgat
    15961 catcgttaat tttatttaaa aagaggtttc tatgagtttc aagaaaattt taggcgttgc
    16021 attggtttct gcattagcat taaccgcttg taaagaagag aaaaaggcag aatctaccgc
    16081 tgctccggca gctcaagctc cggctaaaat caaagtaggg gtaatgtcag gccctgagca
    16141 taccgttgca gaacgtgcag cacagattgc aaaagaaaaa tatggtttag aagttgagtt
    16201 cgttttattt aacgactacg ctttaccgaa tactgcagta agtaaaggtg atttagatgc
    16261 aaacgcattc caacacaaac catatttaga taaagacagc caatcaaaag gtttaaacaa
    16321 cttagtgatt gtgggtaaca cctttgttta tccgttagcg ggctactcta aaaaagtgaa
    16381 aaatgtgtct gagttagcag aaggtgcggt aattgcagta ccaaatgatc cttcaaactt
    16441 agctcgtgct ttgattttat tagaaaaaca ggggttaatt aaattaaaag ataataccaa
    16501 tttattctca acttcagtgg atattatcga aaatccgaaa aacttaaaaa ttaaagaagt
    16561 ggatacttca attgccgcaa aagcgttaga tgacgtagat ttagcggtgg taaataacac
    16621 ttatgccggg caggtaggct taaataccca agatcacggt gtatttgttg agtctaaaga
    16681 ttcaccgtat gtgaatatta ttgtggctcg ccaagacaat aaagatgcag ctaatgtaca
    16741 aaactttatt aaatcttacc aaaccgaaga agtgtaccaa gaagcacaaa aacactttaa
    16801 agatggtgta gtaaaaggtt ggtaatttag ctgattttta cttaaatggc gattaagcgc
    16861 taggttaatc gccattttca tctttaaaat ataggaagga gcaaacaatg aactttaaaa
    16921 aattattagg tgtggcgtta gtatctgcct tagcacttac tgcgtgtaaa gatgaaaaag
    16981 cacaagcacc tgctacaaca gctaaaactg aaaacaaagc cccattaaaa gtgggtgtga
    17041 tgaccggccc tgaagcccaa atgaccgaag tggcagtgaa aattgcaaaa gagaaatatg
    17101 gcttagatgt agagttagtg cagtttactg aatacactca accaaatgcc gcacttcatt
    17161 ctaaagattt agatgctaac gcgttccaaa cggtgcctta tttagagcaa gaagtgaaag
    17221 atcgtggtta taaattagcg attatcggca atacgctagt atggccaatc gcggcttatt
    17281 ctaaaaaaat taaaaacatt tccgagttaa aagacggagc gactgttgcg attccaaaca
    17341 atgcaagtaa tactgctcgt gcgttattat tgcttcaagc tcacggttta ttgaaattaa
    17401 aagatccgaa aaatgtgttt gctaccgaaa acgatattat cgaaaacccg aaaaatatca
    17461 aaatcgtaca ggcggatacc tcacttttaa cccgtatgtt agatgatgta gaacttgcgg
    17521 taatcaacaa cacttacgca ggtcaagctg ggttaagtcc ggataaagac ggtattattg
    17581 tggaatctaa agattcaccg tatgtgaatt tagtggtaag tcgtgaagat aataaagatg
    17641 acccacgctt acaaactttt gtgaagtcat tccaaaccga agaagtattc caagaagcgt
    17701 taaaattatt taacggtggt gtggtgaaag gttggtaatc agttggacgg catattctgc
    17761 cgtccttttc ttaaaaggag aaacagataa tgaaaataat gaaattagcc ggtgcagttg
    17821 caattttctc gcttttttta accgcttgta atgataaagc cgaaaagttg aaagtcggtg
    17881 tgatttccgg ccctgaacat aaggtaatgg aagtggcggc aaaaattgca aaggaaaaat
    17941 ataaccgtga tgttgaatta gtggtattta ccgattatgc cacgcctaat gcagctttag
    18001 ataaaggcga tcttgatttg aatgctttcc agcataaacc ttatttagat aaccaaattc
    18061 aggaaaaagg ctataaatta gtgccggtcg gcaatacttt tgtttatccg attgcggctt
    18121 attccaaaaa aattaaatcg ctggcagagt tgaaagatgg tgatactatt gcagtgccta
    18181 atgatccgac taatttagcc cgtgctttaa ttttattgga aaaacaagat ttaattaagc
    18241 tgcgagcaga tgcaggctta aaagcaacca gtgtggatat tattgaaaac cctcgtaaat
    18301 tggtgatcca agaaattgaa gcaccattat tgcctcgaac gttggacgat gttgcctttt
    18361 cgattattaa tactacctac gcagggcaaa acggtttaac gccaaccaaa gacggaatat
    18421 tcgttgaaga caaggattcg ccttatgtga atttaattgt tgcccgtgaa aataaccaac
    18481 attctgaagc cgtaaaagat ttggtgaaag cctaccaaac agaagaggtg tataacaaag
    18541 cgaatgaaga gtttaaaggg gcgatgataa aaggctggta attgaagata aataaggcat
    18601 agattcctat gccttacttt ttatgttaca gacctgctcc ggctcttaat gcttcggctt
    18661 tgtcagtttt ttcccaagga aaatgtttac gaccgaagtg tccgtaggca gcggtttcac
    18721 ggtagattgg tttgattaaa tctaacattt tgattaagcc atacgggcgt aagtcaaagt
    18781 tttggtagat taatttcaca agtatttcgt tgctcacttt acctgtgccg aaggtttcga
    18841 ccataattga tgttgggtca gccaccccga tagcataaga aagttggatc tcacagcgat
    18901 cggctaagcc tgcggcaacg atattttttg cgacataacg agcagcataa gcagcagagc
    18961 gatccacttt tgacgggtct ttaccagaga atgcaccacc accgtggcga gccgcaccac
    19021 cgtaggtatc tacaatgatt ttacgcccgg ttaaaccaca gtcacccatt ggaccgccaa
    19081 tcacaaaacg accggttggg ttgatgaaat atttggtgtt ttggctgagc cattcgcttg
    19141 gtagtaccgg tttgatgatc tcttccatca ccccttcaat taactctttt tgcgaaatat
    19201 gctctgcgtg ttgtgtagaa agcactacgg catcaatgcc gataatttta ttgttctcat
    19261 aagcaaatgt taattggctt ttagcgtcag ggcgtaacca atcgagtttg cccgatttac
    19321 gcacttttga ctgctgttcc attaagcggt gtgcgtaagt aattggagcc ggcattaaca
    19381 cctcggtttc attggtagcg tagccaaaca taatcccttg gtcgcccgca ccttgctcta
    19441 acggatcggc acggtcaacg ccctggttga tgtccggaga ttgtttacca atcgcattta
    19501 ataccgcaca agagtgagca tcaaagccca tatcagagtg agtatagccg atgtcgcaaa
    19561 tcacttggcg ggttaagttt tcaatatcca cccacgcaga ggtagtgatt tctcccccta
    19621 ctaatgccat accggttttc acataagttt cacaagcaac gcgtgcttta gggtcttgtt
    19681 ttaaaatttc atctaatacc gcgtctgaaa tttggtctgc gattttatcc ggatgccctt
    19741 ctgaaaccga ttcagaagta aataaattaa ttgccatttg tattcctctc tatcttaaat
    19801 atggattatt aatagtaggg acgggtccag tgcccgccca taaattatat aaaatgtttg
    19861 gacaggcaca ggaccggtcc ctataaaatt tgtttggaag tgtttccgtc tagacgtcta
    19921 taatatattt tttgaacgtt ttaggcaaat agaatttgtt tatttggcta aatatgccct
    19981 taaaccagcc aacgcatttt tggcatcttc tacccctaaa tcgtacattg cttgtatttt
    20041 atttacatcc gtttcaaggc gactgatttt gactgttctg cttggacgaa tcacaaaaat
    20101 ctgttgtttt tcattgagtt gaataatctg ctcaacggct tgattatatt ccgcatagcg
    20161 gttttcccag cgttgcacta atttcggata ttttttataa aaacgtttaa acagccacat
    20221 cgagcttggt ttcttacggt attcaagtgg gcgagtaagc accacgataa ttttgtcata
    20281 tcccatttca ataaatttct gcaccggaat actgtctgac acaccaccgt ctaaatattt
    20341 tttaccctca atttcgacta ttttggatac aatcggcatt gaggaagtag ctcgaaaggc
    20401 ttccatttgc tcgaacacat tgtcaatttt gatgtattcg ggctcaccgg tttcgacatt
    20461 ggttacagta acccaaaagt ccataccgga atcctcaaag gcttgttggt caaacggatc
    20521 cagtttggaa gggagttcat agtaagcaaa atcccgatta acgatattgc cggtggtaag
    20581 caagctgtgt aaccccatat accgcttatc tttagcgtat tttttgttgt atcgcaacgc
    20641 cctacctttt tgttttgaag gcaaattcac gccaaataac gcaccggctg aaacaccgat
    20701 tgctccatct atatgaatat gttcatctaa aaatacatct aacacgccgg cagtaaaaat
    20761 acctcgcaaa ccaccgcctt ccagcactaa tccaactttc atatcattct ccctgtaatt
    20821 tgtgtattat aacccgaatt atcttgctag gagaaatcac aatggctgat gcagaattta
    20881 tggatttcct gttagatcaa ctaagcccga tttccggttt gcgttcgaaa aaaatgtttg
    20941 gtgaatattg cttgtttttt gataataaaa ttgtcgctat tatcagcaaa gattatcgca
    21001 tttttgtcaa agctaatccg caaaccttgc cgttattctt agctgaaaat gccgaacaat
    21061 ttcactattt tgctaaagga aaagtgcaaa aaatgcacta ctggagcatt ccggaattag
    21121 cgattgaaga agccgatgaa ctggtcaaat ggattcggct aggcttacaa gcggtgtaat
    21181 ttagcgaaaa ttttgtaaaa aaacgctaaa atatgcccgc ttgttagggg gggggaccta
    21241 atatttctat actttaagat tttatattta tgaaccaaaa aaaattagtt tctttgattg
    21301 tcgttattgt gttggcaatc atttctcaaa tttttagctt ttttgataaa aaagagcctt
    21361 caaaagcaca agaaaaatcg acccagagta gtcagtctca tcaagtttca acttcatcac
    21421 tagggaacta tgatgtgcag atgaaaaacg accgtttgaa acagcatatt gtagataccg
    21481 attattacac cttagcgttg tcgtggtcgc cggcgttttg ccaaaaacaa aagcgaaata
    21541 atcacggtaa aattccgtct catttggaat atcaatgtgg tggctcagcg gattttggtt
    21601 gggttattca cggcttatgg ccgcaaagtg gcaaggcaag aagtgttgat gaacaccctc
    21661 gttattgcca aggcgatttg ccgatggtgg cagagcagac gattaagcaa tatttacctg
    21721 aatcgccggg ggcaactttg ttgcaaggcg aatgggaaaa acacggtgct tgtgccttta
    21781 accaagcgga tgattacttc gccaaacaaa aagagctatt taatcagctc aaattaccgg
    21841 ataccgaaat gtctaaatta gcactgttta actggatgcg agccaataac cctcatttgc
    21901 aaaatgtcta tttagggggt agcaaagatg agctgtatat ttgctatgat aaagcatttc
    21961 aaccaatgga ttgcccacgc taatggcaga tcttcgccaa ctgaaaatgc aggttcagcg
    22021 ccaattaaag cgtgatcttg aacgtgcaaa tcactatttt aataccactt ttacgccacc
    22081 aagtattagc tatgcggtgc gtggcgtaaa agccggtgtg gcttatcttg aacgcaacga
    22141 agtacggttt aatcctgtgt tattgcaaga aaatgaacag gcatttatcc gccaagttgt
    22201 gccgcacgaa ctggctcata tcttggttta tcaacatttt gggcgggtac aatctcatgg
    22261 taaagagtgg aaaatgatga tggaaaccgt actgggtgtg ccggcggaaa tttaccattg
    22321 ttttgatacc caaagtgtgc aacagcaatt tagttaccaa tgtgcctgcc agactcacca
    22381 gctttcgatt cgccgacaca atgccgtgat gcgagataaa aaaagctata tttgccgttt
    22441 atgtaaaact caactccatt ttgtagaaaa aagttaatgt ggagaaaagt taagagaatg
    22501 ttacgattta gctagacatt cctgtttaaa cgcctacaat ttgccgtagt ttttattttt
    22561 aattttattt ccggagtatt tatggaaaca attgtaattg taggtggcgg tgcaggcggt
    22621 ttggagcttg caacctatct aggcaacaaa ttagggcgta aacaaaaggc aaaagtggtg
    22681 ctgattgacc gcaatgcaac gcatttatgg aaaccactgt tgcacgaggt ggcaaccggt
    22741 actattgatg agggaactga tgcgttaagc tatcgggctc acgctaaaaa tcatagtttt
    22801 gaatttcagc aaggtacgct ggtggcggta gatagagaga agaaaacggt ttcactcgcc
    22861 ccgatttata atgatcaaaa ccaacttctc gttaaagaac gccatatcag ttatgacaaa
    22921 ttagtgatcg cgatcggcag taaatccaat gattttggca ccaaaggggt tgcagaaaat
    22981 tgcattttct tagatggctc agagcaagca aaagatttcc aaaaacgaat gatggagctg
    23041 tttttacgtt tttcacatag tgaaaataaa gatgtaaaaa ttgcgattgt ggggggggga
    23101 gcaacaggta ttgagctttc agcggagctt taccacgtgg tgaaaaattt aaattcttac
    23161 ggctttggta aattaaaccg tgccagcttg aaagtcactt taattgaggc aggccctcgt
    23221 ttgattccgg cattaccgga gaaagtgtcg gtttctgctt tccacgaatt gaaaaaagcg
    23281 ggtgtttcgg tgcgtttagg tacgatgatt accgaagctc gtccagatgg gctaatgacc
    23341 cgtttaggtg agaaaattga ggcggatatt atggtttggg cggctggcgt gaaagcccct
    23401 gaaattaccc gtgaatttgg ttttgaaact aaccgcttga accaaattga gattaaagac
    23461 acgctacaaa ccaccgttga tgagtcagtg tttgtcattg gcgattgtgc atcattaatt
    23521 caagatggca agccaattcc acccagagca caagcagccc accaaatggc aacccgatgt
    23581 ggtaaaaata ttgtggcatt gatagaagga aaagagatga agccgtttcg atttaacgat
    23641 aaaggctcac tgctttcctt ctcaaaattc ggcacggtgg gtaatttaat ggggaattta
    23701 gtgagcggtg atatgtttat tgaaggtaaa attgcccgct tggcttacct ctcgctttat
    23761 cgaatgcacc aagtggcatt acacggctgt gtcaaaaccg gcttgattgt gttggtaggg
    23821 caaattaacc gtttcttaag accaacaatg aaactacatt aaacaaaaat ccgcatcaaa
    23881 tgatgcggat tttttatggc aaaataacaa gcggttattt tcttgcctat ttttgcaaat
    23941 actcacgcct agtttgctaa tttaatttcc gccaaccatt tatcaattaa gcgtttgccc
    24001 tcttcgctgg aacctgcttt ttggtaatca atttccacaa agtctaacgc ttcggctttg
    24061 gttgattgtg gagcaacttc ggcgttgatg tttgttgggg tttggtaaac accgtgtttt
    24121 ttccaaggaa tttcctgtgc ttcttttgaa agcacccaat ccataaagag tttggcgtta
    24181 tctaaattac gggcattttt gataatgctg acaccgccta acgcatagcc cactttacct
    24241 tccggaataa tggcttcaat aggcgcacct ttttcttttt cagtggcata actatgtaca
    24301 aagcctaccg taaccgcact ttcaccacga gcaagattgc tggtgactag ggtgcttttc
    24361 acatattgcg aaacgttatt gttgagtttt ttcagataat caaaggtttt ctcttcgccc
    24421 cataaggttg aaagagtggt cataaacgta taagtcgtgc cggagctacg aggatcaggc
    24481 agttgtactt caccttttaa gcgaggatct gataaatctt cccatttttt cggtgcctca
    24541 atgcctagtt tggcgagttt ttcggtattc acgcctaacc ctaatacaag catataaacg
    24601 atagaggtaa atttaccttg ttcactttca actaaggatt taaattgtgg cagaatttca
    24661 gcttgttttg gtgaacggta ggcttccagt aagcctaatt cgcctgcttg aaagtgaggt
    24721 tcaatcgtgc cgccatacca aatatctgcc tgagggttcg atgcttccgc ttttactttt
    24781 ccgaaaattg taccggttcc gccgtgaatg aatttagttt cgacattata tttgtgtgaa
    24841 aattgcttgg tcatttcttc acaaacatta ttttgtaccg agcaataaac agttaaacga
    24901 ccttctgctt gtgctgcttg agctgataag aaaatgccag aggcgattaa gatcgatttt
    24961 aatgagatta atttgagtac gttcatcact ttcctgaaaa cataaaataa aaggtagtga
    25021 agtctagcat ttcaaagcgc tggttgaaaa tagttaattg caatgaagta gggcaattgt
    25081 gcctattact ttaatattta ccaactattt gcaagtttta gcaaaaaata accgcttgta
    25141 gtagctcttg ctattttgtc aaaattcaga ataataaatg cctttagtct aatgttaaat
    25201 tttagttatg gaaaatagtt ggaaatatgc ttttaaatta atcacgccga tttttatggg
    25261 ctattttgcg gcaggggttg cttacggaat gcttgccacc aatgccggaa tgcctgcgtg
    25321 gtttacgatt gtaatgagtt ttaccgtggt atcggggacg gcacaatatg cagcgattcc
    25381 attttttgtc tccggtgttg gggcggtgtc ggtctttctc agtacgcttt taatgagtct
    25441 acgttttagt ttttacacct taaatatgat ggagcataag ccgatatcca aagcgaaaag
    25501 tctggtcgct attgcaggtt taactgatga aggctttgcg gtgttatctt cattaccgaa
    25561 agagcaaaag caagcattat tctttaaaat cgctgtgcta tgtgtggctt attggacatt
    25621 ttccacgatt gtcggtgtgt tgttaggcga ttcagtggcg aactatattc cgcatttgga
    25681 ttttgctctg ccttgtttgt ttgcaatttt agcctatgag caatacaaaa accaaaaaca
    25741 atggaaaccg attgtgattg cgttaatcgg atttttactt gctcgtcaga ttaccgaaac
    25801 cagcgtgtta ttggtggcaa ttttagtagc gattgtcatt gtggctttat tacctcaatc
    25861 ttcttcaatc aaactagaga aaggagtaga aaatgagcac aaatgatact ttgataatgt
    25921 ttgcagcatt agtgctcggc tcgcaaattt gtcgtttttt gccggaattt ttaccgaaaa
    25981 aggtgttatc ttcgcccatt ttgcaaaaat taaacaaaat gttaccgctt gtgattatgc
    26041 ttttattgct tttaacgagc ctaaactttc ctaaaaacgg ggaaggcttt aacttaatat
    26101 tggcacaaat tctggcttta ataggcgtat taggtagcta tgtttggctt aaaaatacct
    26161 ttttaagcgt agcgttgggg attttcggtg tgaatgtatt tttatccctg ttaggataaa
    26221 taccaaaggt cattttatag gagcgaaaca ccttcgctca atcattaaca gatgtggggc
    26281 gaaaaatttt tcgcccctta tttatcacaa gcggtctttt tctttcaatt ttttgcaaat
    26341 taaatcgcta aatcaaacgc atagaaggca actaaaataa tcgcaataat tactgtacct
    26401 aggtttaatt tattggcttc gcctgaaatt aagcgaccaa tcacaagggt ggcgaaacct
    26461 agcataatgc ctgtcacaat gttggcggtt aatacgatga acaccgcaca gactaaacct
    26521 gacattgcac ctacaaaatc gctgaaatct aatttagaga cattacttag cattaataag
    26581 cccacataca ttaatgccgg tgcggtggca tagcttggta ctaagaatgc aagaggttgg
    26641 aagaacagca taaataagaa accgatacca accactacag cggttaaacc tgttttaccg
    26701 ccaacggctg tacctgctgc cgattcaata tacactgccg ctggtgctgt accaaataag
    26761 cccgataaaa ttgatccgaa tgagtcagaa gttagggctt tatcgccatt gataatttgt
    26821 ccgtctttat ctaataaacc ggcttgccct gcaaccgcac ggatagtacc tgttgcatca
    26881 aaaatagcgg tcattactaa cgcaaacact accggtaaaa tggcggtatt taatgctccc
    26941 ataatatcaa gatttaggaa ttgcgaattt tcaccaaaac tcggcatttt gaaaaagcct
    27001 gtgtatttta cgttagggtc aaaaattaaa cctacgatgg taatagcaat aatgacccat
    27061 aaaatactgc cttttacttt cagcttttct aacccgataa tggcggctag accgattaat
    27121 gacactaaaa ccggaaatga agtgaagtcc cctaatttaa ccggaatatc cataccgcta
    27181 ttgaccacta atttaacgtt agtggcggca ataatcagta agaataaacc aataccaatt
    27241 ccagcaccgt gggcaatgct gcttggcaaa ttacgtaaaa tccaagtacg aataccggta
    27301 gcagaaatca aggtaaatac tacgcccatt aagaaaatcg ccccgagtgc aacaggaata
    27361 gaaacttgtt gccctaatac taagctaaag gcagtaaaag cagtaagtga aatcgcacaa
    27421 ccgattgcca ttggggcatt tgcccataaa ccgattaaga tagagccgag acctgaaact
    27481 aaacaggtgg caataaatac cgattcggca ggaaagcctg cttggcttag cattcccggc
    27541 acaacgatga cggaataaac catggctaaa aaggtagtta aaccggcgat gatttcttga
    27601 cgagcgtttg aaccacgctc ctcaaattta aataacgaca taagaacctc aaaagaagaa
    27661 aatgaaaggg gaaattttag gatgcggaat tttacagaaa tttaaaataa ttagcaaacg
    27721 tttgcgtaaa aagtgaaaga aaagcaggaa taaatcctgc ttttgtttga tggttagtaa
    27781 tcgatgaaag cgtcttgatc tgccccttcc ttttcaactt taggctgaac catatgttca
    27841 cgttttagcc caagttgtag tgccacacca attgcaatgt agatggatga ataagtaccg
    27901 aaaccgatac cgatcaataa tgcaagtgag aaactatgaa gcgttggacc acctagatag
    27961 aataaggcta gtaccacaaa gagggtggtt actgatgtca ttaaagtacg cgaaagtgtt
    28021 tggctcaagg aaatatcgat aatttcaatt gagctggagc gacgaacttt agaaaagttt
    28081 tcacgtacac ggtcaaacac cacaattgag tcatttaacg agtaaccgac aacagataaa
    28141 atcgctgcta caaaggtcaa gtcaatttca atctgtaaga aagagaagat tccgatcgtc
    28201 actaatacat cgtgaaataa agccacaata ccgcctaccg ctaaacgcca ctcaaaacgt
    28261 gttcccacgt aaataagtag catagcaagt gttgccaaag tggcataaat agccccttgg
    28321 gttagctctt cccccacgtt tgggccaacg aattcaatgc tcttgatagt cgcattcgga
    28381 tctaattttt gatggatcat ctccatcact ttattcccaa cagacatatc accggcactg
    28441 gcggcaagtc ggatcattaa atctttttga ctgcctgtgg tttgcactaa ggcattgccg
    28501 tagccgctat tttcaagctc cgtacgcagt tttgctaaat cagcaggttg tgaaaattga
    28561 gtttcgatta ctgtcccgcc ggtaaaatct aacccccagt taaagccttt tgtcgcaata
    28621 gtaaaaatgc ataaagcagt tacaatcagc gaaaaaatga atccgacata gcggtatttc
    28681 ataaagggga ttaatttata aggcagttta atttctgcta catcttgttg tacattttca
    28741 atagccattt ttccacctta aatccaaagt ttcttcacgc gtttgccacc gtaaacccag
    28801 tttacaagca ttcgagtacc tgtaattgcg gtaaacatag aaataattac ccctaatgca
    28861 agcgttaccg caaagccttt taccggacct gtacctacac cataaagcac aagagaagtc
    28921 aaaatagtgg tgaggttgga gtcaaaaata cttgagaatg cgccattata accttcgtgg
    28981 attgcttgtt gaattgggcg accgttacga atctcttctt tgatccgctc gtagattagt
    29041 acgttggcat caactgacat acccacagca agtacgatac cggcaattcc cggcatagta
    29101 agggttgcac cgattaatga cattaaacca atcgttaaga tcatattcac aatcaaggca
    29161 agagaggcaa atataccaaa tactttataa aaggctagac agaacaggat cgttaagcct
    29221 aagcctaaaa tactagcttc cataccttgt ttaacattat ctgccccaag agaagcaccg
    29281 atagtacgct cttccacaat cacgattgga gcaatcaacg cacctgaacg taataatacc
    29341 gcaaggtttt gagcttccgc tggtgaatta atccctgtaa tctggaattg attacctaaa
    29401 cgggtttgaa tggttgcaac gttaattacc tcttggtgtt tttctaaaat gactttaccg
    29461 ttagcatctt tacgacctga atctttaaat tcggtataaa gtgttgccat cggtttttgg
    29521 attgccattt tagtaatttc acccatcatc tcgccgcctt cggtatcgag tcggatactc
    29581 acttgcggca agccacgctc atcagtgcct gaggttgcat caataatatg ttcaccccct
    29641 aacgaaggtt tacggcgtaa tacaactggc tcgccatcac gggtgttttg cagtcctgaa
    29701 tcagccggca caatgccttt cgccgcagag gccacattgg cttcggtatt gactaaccgg
    29761 aactcaaggg ttgcagttgc ccctaaaagc tctttggcac gagcggtatc ttgcacaccc
    29821 ggtaattcaa ctacgatacg atctgcacct tgacgctgaa tggtcggctc agaaacgccc
    29881 aattcctcaa cacgtttacg caaaatagaa aggttttgtt cgattgccat cgtgcgagat
    29941 tcagaaagag cttgtgctga tggcgttaag gtgactttat tatcatttac caaaatatca
    30001 agcgttgggt gagagagacg cactaaacgc actgcttttt cggcggtatc gttatcggca
    30061 aattcaacct cagttgcaaa attttcgcct tttttgaccg cttgatattg caatttttct
    30121 ttacggaaat cactacgcag gctgtcttgc aaagtgtctt gttgttttgc aagcgccgtg
    30181 ttcatatcca cttccattaa gaaacgaaca ccgccaagca agtccaagcc tcgcttcatc
    30241 gggctaccgc caatgtcaga caaccactgt ggcgttgctg gtgcaaggtt taaggcaaca
    30301 gagtagttat tccccaatgc ttctgagatt ttttctttgg caggcaattg ctgttcgctg
    30361 ctttcgagac gaaccaaaat cgagccattt tctaacacgg cagatttcgg ctgaatattc
    30421 attgctgaaa gtgtggattg cacttgagac agtgtctcag gtgtggcttg ttgcccacga
    30481 gtaccggaaa tttgtaccga aggatcatcg ccatataaat ttggaagggc atataaagca
    30541 ccaatggcga tcacgaagat caccattaaa ttcttccata gagggaaacg gtttaacaca
    30601 ataactcccc gaatgttaaa gttggataag agggttgatt aaagtgattt taatgaacct
    30661 tttggtaaca ccgcaacaac gaaatcacgt ttgatggtca cttcagttgt gtcatttaat
    30721 gcaatcacga tgttatcgtt atcagcggtc actttggtaa ttttaccgat taaaccaccg
    30781 ctggttaata cttcatcgcc ttttgtcaag cttgccagta aatcacgttg ttgtttttgg
    30841 cgttttgcct gaggacgata aatcataaaa tagaaaattg caccgaaaat aatcaggata
    30901 aagatcattt ccattccgcc accttgttgc ataaaaactc cttaaatgtt tgagtggtat
    30961 aaaaataagt gttataaaaa acgcctgtta aaataccata aattcaatga attaacgagt
    31021 agaaaaattc atcgaatgct tttcttttaa acaaaaattt gagtagaatc agcactttta
    31081 gtttaggatt aggttcttgg cgtgttcttg cgctgacaca ataggagaaa aaatgaaaaa
    31141 attattcgct caagcggtta tttttggggc tttttttgca agttccgctt ttgctcacaa
    31201 tgtgcaatta aatcaagcat tacctgctgt ttcggttgca aaagacgggg aattggcagt
    31261 ggcaggtgga aaggttagct ataaaaattg gcattcatca tctctggcag gtaaagtccg
    31321 tgttattcat cattttgccg gtagaagttc ggtgaaagaa aaaaatgaag atttgatgac
    31381 agcaattaaa aatgccggtt ttgatcgctc gaaataccaa acggtgacga ttgtaaatgc
    31441 tgatgatgcg attgtcggca caggggtatt tgtgaaaaat agtgtggaaa acggcaaact
    31501 tgacaatcct cacagccaag tagtgttaga ccagcaaggc agtgtgaaaa aagcgtgggg
    31561 cttaaaagag aaagagagct ttattgcagt gctggataaa tcgggtaagg tacaatttac
    31621 ctctgagggc aaactctctg caaaacagat tcaagaagtg attgggttgg tgaaaacatt
    31681 aacgcaataa ccagtgtttt tatgggcgga ttttgtatcc gcccatcatt ctagataata
    31741 aatcgataac ttatcgtgtg atgatactct tctcctgctt ttgttatccc gccaaattgg
    31801 agtaattcag gctggtttag tgaatttggg aaaaactcag gctctaatgc tacaccagcg
    31861 taatcttcat attcgccttt agtcaaatta ggttgtcctt ttagccagtt gccggtgtaa
    31921 agttgtaatg ctggcatgga ggtgcggagt tccattttta agtcttccac ggctaaacaa
    31981 gcggtcggat tttcactatt ttttgcaaga tgaaaggagt gatcgtaacc ttttaccgct
    32041 ttttggtcgt catcagccaa taaatcttgc ccaatttttt tagcattgcg gaagtcaaaa
    32101 ctggtactct ccactttttt cagtggagca ttaggaatac cgctggcatc aaccggtaag
    32161 tatccgtctg cattaattat cagctcgtga tctaaaatcg tctctgaacc ttgcaaatta
    32221 aaataggcgt gattagttaa gcaaagcggt gtatcttggt tagcaacggc atcaaagctg
    32281 atttgaagct cattctctgt taagcgatac tccacaaaag ctttcacttc gccgccaaat
    32341 ccttcctcgc catctgcaaa aaaataacta aatttgaccg cttgttcgct ttggctttca
    32401 agttgccaca ctattttatc tgccccaagc tcgccaccgt ggagattatg ttcaccgtta
    32461 tttttagcga gctggtaggt tttgccgttt aagcgatatt cggcattggc aatgcggtta
    32521 gcgtaacgcc caatggtcgc tccaaagaaa gcggtctgtt tttgccaatt ttttgcagac
    32581 gtggtgacta atacttcccg ttcctcgttc ggcattttga caatgcacga aagccaagaa
    32641 gccccaacat cagaaagggt aatgcgtaaa aacgcatttt caagggtgaa taatttcatc
    32701 ggttgacctt aattacctag cacggcatag ccgtactagg ttaagcataa ttcagccctg
    32761 ctagggctga ggttatttat tttagagtag cggagctact tccttatgct taacccggta
    32821 cgctttagcg tgccgggcat tctcgcaccc catcggaggc agtacaaacg tggaaggttt
    32881 ctttaatgcc ggtttgcttt tcgtaattgt tagcaacggc ttggcggaga tcttccactt
    32941 tgtcatttgg cactaggcac acgacacaac cgccaaagcc gccgccagtc attctggctc
    33001 cgccgttttt accgatagct tgttgggcga tttcggctaa gcaatccact tcagggcagg
    33061 tgatttcaaa atcatcacgc attgagtcgt gagattgagc catcaactcc ccgagtttga
    33121 ccatatcatt cgcttttaag gcctctactg cttccagaac tcgttgattt tcgctgatga
    33181 tatgttttgc tcgtttatac gccagctcat tctcttcact aagttctgag gctcgttcaa
    33241 taaattgttc cgctgtgaca tcacgcaatt ttgctttgcc gaagaagtgg gcggcaaatt
    33301 cgcactgttc acggcgactg ttgtattcgc cggtaaccaa gtcgtgtttt acgttggagt
    33361 taataatcgc aatcgaatag ccttgtggca caggggttgg ggtgatttct aaactacggc
    33421 agtcgatcat cactaaatga tcttgttgcc cgagagcgga ggtgagctga tccatattgc
    33481 cgcagttgca gccgacaaat ttattctcag cctcttgccc gattaatgcg atttgcggta
    33541 aagaaagcgg taaatcgccg agtaattgag cggttttacc aatcgacact tccaatgcgg
    33601 cggaagaact taaacctgat gacatcggca catcgctggt aatggctaaa tctgctcctt
    33661 gtttgaattc ggggtgtttg gcttgaataa atttcaccac gccacgcaca taattcgccc
    33721 atttttgctc gcttggctca atcgcctggc ttaaatcaaa ttcatcttgt tggttgagat
    33781 caatggcgaa cacccgccat ttgttgtcac tacgtttggc aaagctaacc gctgtgccgt
    33841 aattgatggc acaaggcatt acaaacccat cgttgtaatc agtatgttcg ccgataatat
    33901 tcactctgcc cggggcaaat accgtgcgtt ctgctttata accaaaatgt tgttcaaatt
    33961 gttgcaaagc gatttcttgt ggtttcattt caattcctta tttatcttta tagtgtttgg
    34021 tatcacttac tgcatttaaa cgttcggcag cttgttccgg ggttaaatca cgttggcttt
    34081 ctgccatcat ttcataaccg accataaatt tacgcactgt tgccgaacgc aagagcggcg
    34141 gatagaagtg agcgtgtaat tgccagtgag cattttcctt gccgttaaac ggagcgaagt
    34201 gaaagcccat tgaataagga aagctgatgt taaacagatt gtcgtaacgg gtggtgagct
    34261 ttttcaatgc aagggctaaa tccgcttttt ccgcttcgtt taaatcggta atggatttga
    34321 actgcttggc tttcggcaac agcaaggttt caaacggcca agccgcccag taaggcacaa
    34381 ccgcaatcca atcttcggtt tctgccacaa ttcgctcttg tttgtctaat tcacgttgtg
    34441 cgtagtcaag caacagtggc gagccatatt gagcaaaata tttcgcttgg cattcgtctt
    34501 caatcgcaat ttcattaggt agaaaattac ttgcccaaat ttgcccgtgt gggtgagggt
    34561 tagaacaccc catcattgag cctttgttct caaaaatttg cacccactga taatgctctt
    34621 tcagctcatt cgcttgctct tgccaaactg ttaccacatc agtaatttcc tcgatggaaa
    34681 gttgcggtaa ggttttacta tgatcaggcg aaaaacagat cacacggctt tcgccttgtg
    34741 tatgagaaat ctgaaacagc ggatcatcat tcggtattgg tgccggtgtg tctggtagca
    34801 gggcagagaa gtcgttttta aacacaaacg gcttggtgta attcgggttt tgttcgccgg
    34861 taatccgctt attgccgggg cagaggtaac aacttgggtc gtagctcggt ttttcatctt
    34921 gcacgatttc ttcttgttgc ccttgccacg ggcgttttgc ccgatgcgga gagactaaaa
    34981 tccattgatt ttttaatgga ttaaaacgac gatgtgggtg atcgttgagg acaaattcgc
    35041 tcatataaaa ctcctcgaaa ttagttatgt aaagagtata gcgaaagtca gacaaaatga
    35101 ctgtgacata gctcacaaaa taatgatgtc aagcggtcaa atttcagaaa aattttgcaa
    35161 gttttcctcc attttgcgat ttaaggtagc ttacgtggaa ttgaatgtgg cttttggtag
    35221 aatagatgaa tatgcaagat attaagacaa aaatagatgg aaagactgaa taaatttgat
    35281 gcttggttaa tgaatcataa acctaatgct aggttaagcg gagcgagtcg tttgttgaca
    35341 gagttttggt tctttgggtt aaaagaagct agagcctgtt tatttgcggg attatttttt
    35401 attgcgatgt tttcggtacc aaaaacggga ttatggggct tacctcgtta tgatattttg
    35461 ctgatttttg ccatagtggt gcaaattgca atggtgaaac tggggctgga aacgtgggac
    35521 gagctaaaag caataagcct atttcatttg gttggttttg tgttagaagt ctttaaaaca
    35581 tcgagtaata ttcaatcttg ggcatatccg gattttgcct atactaaatt gttcaatgtg
    35641 ccattattta ctggttttat gtatgcagca gttggttctt acattatcca agcgtggcgg
    35701 ttgtttgatt tgaaaattaa aagccacctg ccctattggt tagccacttt aacggcactg
    35761 atgatttacc tgaatttctt tacgcatcat tacattgggg attaccgttg gtatattgcc
    35821 gcttttgcat tagggctgta tgcacgtact tttgtatttt tcacccctta tgacattaca
    35881 cgaaaaatgc cactgctgct ttctttcttc ctgataggtt ttttcatttg gttggcggaa
    35941 aatatcggta cgtttgccgg aatttggcgc tacccgaatc agtttggggc ttggtctatg
    36001 gtgcatatga gcaagtggag tgcttgggcg ttattggtga tgatgacttt taccatcatt
    36061 gtgcatttaa agcatatcaa gcaaacagtc agagtctcga gcgattaaca agctgtgggt
    36121 tttggcgaaa attttactca tcagttattt tcagtggaat taggggcaaa taataatttg
    36181 cccctatatc aaattatgga ttttcccctt ccacatcaat aatcatcact tccgactgtg
    36241 agcctaaccg catcggcacg ccccagaatc cgtagccgga ggtgacaaaa taatgcccga
    36301 agccgatttt ttcatagccg taatgtaatc gatagatcag tttggtaata atacttgcag
    36361 ggaacacttg ccctttgtga gcgtgtcctg aaacttgaat atcaatcggc aatttagcat
    36421 gttgctcaat ttcagtcggg cggtggtcaa gcaaaattac cggtagttta gtgttcacgt
    36481 tttgtaaaag ctgttcggca gaaggtcgat ttttgactaa atcatcatta cgcccaataa
    36541 gggtaaattt tccgttaatt tcggccgttt gatcccataa aacagtaata ccggcgtcgg
    36601 taatttcttt tgcaatcgtt tcttgagccc caaaaaagtc gtgattgcca agggtagcat
    36661 acacgccaag aggagctttt aatttggcta aatgaggctg cattttatcg gcaacataaa
    36721 cttcggtatt gtcgtccata atatcgcccg gcaataaaat cagatcgact ttttgctggt
    36781 tgaaaatctc tgccagttgg tcaagttgct tggttccgaa cagttttcct aaatgcagat
    36841 cgcttgccat accaatgcga agcggtttct ctaatggttt atccaatttc accgaataat
    36901 gtactactgt tggggtataa gcgttgtaat agccaagccc aagtaagcca aagaatgcaa
    36961 atgggtaggc gattttcagc cagcgattga cggtagtatt gccgttgaat attttgtaaa
    37021 tacaagcggt gaaaaaactg ataaaaaatg caaaaagtaa caataccaat atgaatgcca
    37081 ttattctaaa tagcgcaaaa gtacgagtaa gatgcaaaat cactaatccg tttgcgacta
    37141 agaaagtgag aataccaact gttttacggt ttttacttga taaattaaat aaccagctga
    37201 cagtctttgc aaagttgaat aaaaagagtt gtaacaccgc aatcacaagg gcggtggtaa
    37261 tgtagtaacg atattccata tttttccttc aataaattgg ctaaacccag ccgatttgtc
    37321 ttaataaaaa taagccgatg ctggcactaa acattgagcc gagagtcgta atgccgatca
    37381 gatttgcggc tgcctcgccg tctccgccat agttgcgtgt cattgcgtag gtggctgaag
    37441 caacgggagc agaagcagcc acaaagacaa tgcctagttc catcggcgga agttgaaaca
    37501 caaatttccc gagtagtagc aagaaaattg gagcaaaaat cagccgaatc gaggcagaaa
    37561 acagcacaat tttattgatg gtgctttcca ctccttgatt aaattgtttc aactgtttga
    37621 aattaatgct tgctccggtg cagataagtg ctaatggtag tgtcaggctc gccattaatt
    37681 taccagcaga ctcaagcggg gttggaattt ggtggcttaa ttcaaaatag ttaaaagtta
    37741 agcctgcaat gacggctaga atcagcggat ttctcaatat cgacaatatt aatctgccaa
    37801 aattcacttt tttctcgctt aacgatgaag tgagggtaat cactgcaagc acattaaagg
    37861 taatcaccaa gcacgaaata taaactgaag cggcagcgag agctttatcg ccataagcat
    37921 tgagaattaa ggcaatgcct aaaatcgccg cattggtacg gaaagtgcct tgagtgaaaa
    37981 tacaacgata acggcggttt tcaatatgtc gagccgccca ccattccgcc cataaataga
    38041 tcaaaatcga accgccgact ccggtcgaga tcaggttgat ttggctggag aaatctaacg
    38101 ggctttttag catattcaag aaaatcatcg gtggcagggt aaaattaaat accacctttg
    38161 atgccatatt gcaaaaggta tcgtcaataa attttcgtct gcgtggaaaa ataccgaaca
    38221 gcattaacaa aatggttggt aaggtaacgc caatgctaaa ttggatagag tcgaaaagca
    38281 taatgtttgc cttaaatgat ggcacgagtg tcttttgctt gtaccgtaga ttatcaataa
    38341 agtaccagcg tggacgctgg tactatcatc aatggaaatg aaattacaat cctgccttta
    38401 agcttgcttc gacaaattta tccaaatcac cgtccagcac ttgttgggtg gtgcgatgtt
    38461 ccacaccggt acggtggtct ttaatccgtg aatcgtctag cacataagag cggatctggc
    38521 tgccccagcc gatgtcggat ttgttgtctt ccatcgcctg tttttccgca tttttcttgc
    38581 tcatttccag ctcatatagc ttcgctttaa gctgtttcat cgcctgatct ttattttgat
    38641 gttgtgaacg accgttctgg cactgcacca caatgccgct cggtatatgg gtaattcgca
    38701 ccgccgattc ggttttattg acgtgctgac cacccgcccc tgaggcacga tacacatcaa
    38761 tccgcaaatc cgcagggttg atttcaatat caatatcgtc atcgacttcc ggataaacga
    38821 aagccgccga gaacgaggta tgacgaccgt tattggcatc aaacgggctt ttacgcacca
    38881 agcggtgaat acccgtttcg gttctgaacc agccgtaagc atagtcgccg gaaattttca
    38941 cggtggcgga tttaatccct gccacatcac cgtctgaggc ttccattagt tcggctttaa
    39001 accctttgct ctctgcccaa cgcaaataca tacgcagtaa catttctgtc cagtcctgag
    39061 cttccgttcc acccgaaccg gcttgcaaat ccaaatagca atcggcactg tcgtgtgggc
    39121 cgctgaacat ccgcttaaat tctaaatcgg caagtttttt ctctaattcg tctgcttcca
    39181 cttgggcttc attgaaggta tcctgatctt ctgcttccac cgccagctca atcaaccctt
    39241 caacatcttc aatcccttgc tctaaggctt taatcgtatt gacgatctgc tccaacattg
    39301 cccgctcttt accaagcgct tgtgcttttt ccggcttgtt ccatacatcg ggttgttcca
    39361 gctcggcatt gacctcttct aagcgttcga ctttatgctc aaagtcaaag ataccccctg
    39421 atcaccgcgg tgcgctcgct taaatcagtt agttggtttt taattgggtt taattcaaac
    39481 attgtcgttc ctgtttttgc ttattttaaa aagatgaatt atagctgaag ttaagcaagc
    39541 ggtcgaattt tgcaagaaat ggagcaatga tcgtttctag taatgagatt tggttcataa
    39601 aagttgcaac tgtttctgct atttggcttt gatagctaaa aatctttaaa aaaacaagcg
    39661 gtcgttttac cccatttttt gcaaaacgac cgtagcttct tgctataatc gctgaaaaaa
    39721 tgctgcaagg agattttatg gcaaagcgcc caagtgatat tgatgcaacc ctattgcaac
    39781 tggaaatcct acgccgtatc cccaaattac ccgctaaaat tgatgctaaa agtttgcacc
    39841 aacagctatt agatgccggc tttgaacggg atattcgcac tgttcagcgt tcgctcaaaa
    39901 tgttatgcga gcattttgat attgaatgcg atgagcggag caaacccttt ggttatagct
    39961 ggaaaatccg ctcagagggg ctggcattgc cgattctcaa cgaacaacaa tcattactgc
    40021 taaaacttgc cgagcaacag ctgaaatatc tgttacccgc aaatttaatg gcatcaatga
    40081 aacccttttt tgagcaggca gacagaatgg tttccggggc gaaagacaag cccgaatacc
    40141 aatggctaaa taaagtttgc tccacgccaa ccagcctacc gctgatccct gcaaaaatta
    40201 aagaagagat ttttaattcc gtaagccaag ccttatttca aaataagttg ttaaagatcg
    40261 aatatcagaa ccagcacggc aaaaaacacc aagcgacaat tatgccgttg gcgatttcac
    40321 aacaaggtgc gagcatttat ttggtctgcc gttacgaagg gtttgacgac aaccgcctgt
    40381 tggctcttca ccgcattcgt aaagcggaga tttctacttt tagttttgaa cgcccgaaag
    40441 attttaattt gcaagcctac caagatgccg gacacctcgg ttttggcaat ggcgaaaaag
    40501 ctagccttac cttttcgatt agcagagacg ccggtttcca tttaaccgaa acaccgcttg
    40561 ccaaagatca aaaaattctc gaggagagtg aaactcacta ccgtattcaa gccatagtcg
    40621 ctgataatga tatgctaacc tggtagctac gcaaatttgg cgaagatatt tgggatatcg
    40681 agaaagaagc gctaaaataa gctaaacaag cggtcggttt tgtgggaatt tttgctaaat
    40741 agtatcgacc gctcctccct aaacagacct ttatcataaa tacaactctt tcaatcagat
    40801 attcttactg tctattgatg aacgattcga atttttctag tggttgaaat ttaaactaat
    40861 tttcttgctt tttggcattt ttcagacttt taattctctc cattagcctg tttttttacg
    40921 ctctttttca taaacaggaa taaccaatgc cttttcaact tactttctgc caacaagccg
    40981 gcaatcagca ccagaaaaat caagatgcac tgtttaacgg caaagcggtg taccaatggc
    41041 aacttaaaaa tgccgagagt tatctgctgg ataacgacaa agtaattttt ggggttgcgg
    41101 acggcgtttc cagccaccct cgaaatcatc ttgcctcaca ttatttaatg gcgttactca
    41161 gccaatgcca atcccttgat agccaatggt tgcgacaggc tcacggcgat ttttgcgaaa
    41221 aattagcgaa agattatttt ggtgcttctt gcacttttgt tgcttgtgaa ttacaccata
    41281 acggcagagg gcgtatggtt aatgtgggcg acagtcgagc ctataaaatt acggcaaacg
    41341 gggagtggca acaactgagt tttgatcaca ccatactggc tgaaatgaaa cagaaaggct
    41401 tggttaagga agggagcaac tatgcctcaa tgtataacgg cttaaccgat tgtattgttg
    41461 ccgattttga cgccgatgat ttccgcattc atatagccga atttcaactg gaaaaaggcg
    41521 agagcatttt gctctgttct gacggtttgc acgaacaact cgccccagaa gctattcagg
    41581 cgatttagga cggatacgaa agcccgtctc atcgtctgca aatttgcaga aaaatggtaa
    41641 aaatgcaccg cttgtatgac gatttttctg cggtgatttg tgaaattgtg taattaaaac
    41701 gacattattt gtcgcattgg tgcttaaaat ccgcataaaa cagaggagaa aaaaatgaaa
    41761 aacgatttcc attatgccgc tgaattaatc cgcaatgcag acggcatttt aattaccgca
    41821 ggggctggaa tgtcggtgga cagtggcttg cccgatttcc gtagcgtggg agggttatgg
    41881 aattcctacc caccgctcaa aaaatttaat ttgcaattta tgcagattgc cacaccgctt
    41941 gcctatcagg aacgccctga
//
LOCUS       DANLXS010000003        38601 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.003, whole genome shotgun sequence.
ACCESSION   DANLXS010000003 DANLXS010000000
VERSION     DANLXS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 38601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38601
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.003"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            9..701
                     /gene="uppS"
                     /locus_tag="PXD71_000112"
     CDS             9..701
                     /gene="uppS"
                     /locus_tag="PXD71_000112"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDL6290281.1"
                     /translation="MPKHVAIIMDGNGRWAKQKGKLRIFGHQNGVKAVRSAVSFAAKH
                     HIQVLTLYAFSSENWSRPETEVSALMSLFMKALDSEVKQLHKNNIRLKIIGDKSAFSE
                     NLQKRIAESEKLTENNTGLILNIAANYGGYWDIVQAAQQLAVHVKSEQLAVEQITPAL
                     FQQHLVTENQPQVDLLIRTSGEQRISNFLLWQIAYAELFFSPVLWPDFNDEAFSDAIY
                     AYQQRERRFGGC"
     gene            725..1594
                     /locus_tag="PXD71_000113"
     CDS             725..1594
                     /locus_tag="PXD71_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL6290282.1"
                     /translation="MLKERVLSAIIMIIAVLAAIFWLSPLPLTLVLSAIVVAAMWEWA
                     QFAGFKHPVPRAIVAMVTICLLLFAIVANTDYIRATRFITDETTPILFIGCIWWLIAL
                     VLVATYPNSAKLWEKSVAAKFLFGLATLVPFLIGTLALRFHNYAFDPYQGTYLFLYVC
                     LLVWGADSGAYFAGRAFGKRKLAPKVSPGKSWEGVIGGVLTSSLIAFTFLQLTPANVF
                     GKALATTPFILVSVGMVAISVLGDLAESMFKRQAGIKDSSQLIPGHGGILDRIDSLTA
                     AVPFFATFFFFVL"
     gene            1598..2908
                     /gene="rseP"
                     /locus_tag="PXD71_000114"
     CDS             1598..2908
                     /gene="rseP"
                     /locus_tag="PXD71_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL6290283.1"
                     /translation="MTSIIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVL
                     FKKKDKQGTEFVFSLIPLGGYVQMWNDETDINAPAQQALNTKSVLQRAFIIIAGPAAN
                     FIFAILAYWVVFIAGIPTVKPVIGEILPNTIASQARIPTELEITKIGHQSIQDWESAS
                     LALVGFVGNKNVPLEGNINGNSTQKYELDLSNWSIDSAKENPLTALGIRPKSAKVTAE
                     IKQVVENSPASQVGIQAGDRILQINQQPFNWFNLVELVQAGKPIELKIEQRGQIKDLV
                     VQPEKKDERYIIGVIPSYEPLADKYRTELKYDILTAFYKSIEKVWSLTQTILQFIGNL
                     ISGDLSIKNLGGPISMAKGAGATAEIGLVYYLSFMALISVNLGVMNLFPLLPLDGGQL
                     VLLAVEAIRGKALSEKIQLKFQQIGFAFVLSLMLFAFANDIIHF"
     gene            3023..5404
                     /gene="bamA"
                     /locus_tag="PXD71_000115"
     CDS             3023..5404
                     /gene="bamA"
                     /locus_tag="PXD71_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDL6290284.1"
                     /translation="MKKLLLSSLLIANGVVAAPFVVKDIRVEGVQPTTGESIISSIPV
                     RVGQTATDTDVSNVVRHLFSQQRFADVRATREGNTLVIKVAEKPIIGKVEIEGNQAIP
                     KDALEQNLKANLINQGEIFDAAKLEAFKEGLIEHYHSTGRYEAKIDTSVSNNTEGGVN
                     IKLAINEGEVAKAKTIKFEGNNAFSDKKLLENLDIQPDVSWWNIFESSKFEQTAYQKD
                     LETLRDFYLNRGYAKFAIQDTDVQFNDNKTEVALTYKLDEGSQYHVSEMRIIGNTANM
                     DSQLNALLKEFKPGQLFRKSDLTQIEEEIKSILGDAGYGSAKVDIYPQFNEENKTVKI
                     SFVVDAGRRIYVRKIRFEGNGVTADSTLRREMRQQEGAWLSTNKASLGKARLERTGFY
                     ESVDMSMPNVEGTADQVDVVYKIKERNTGSINFGIGYGTKNGISYQAGIKQDNFLGMG
                     STISLNGTRNNYGTSVNLGYTEPYFTKDGVSLGGNIFYEDYDNSDNDSMASYKRQTYG
                     INGTLGFPVNENNSYYLGLGYTHDKIKNAAREYTREKYVKSMNFEFDPAADYYKKIKA
                     DDFDFSLGWNYNSLNRGYFPTEGTTANIGGKITIPGSDNKYYRVSADFRNYYPLNREH
                     KWVISTKASIAYTNGFGGKEVPFYQLYTAGGIGTLRGFSYGAVGPQAIYYNTKSGKFD
                     SMNNDIIGGNALAAASLELITPTPFVSEKYQHNVRTSLFVDAASAWNTKWKKDEYTNL
                     PDYGDFKRFRASAGIAFQWMSPIGPLSFSYAKPIRKYDNDDIEQFQFNIGSSF"
     gene            5484..6275
                     /locus_tag="PXD71_000116"
     CDS             5484..6275
                     /locus_tag="PXD71_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDL6290285.1"
                     /translation="MKKLFKMTGLAVALASTTGIANAADTIGFVDPSYVLQNHPVLLD
                     ASAKFDKFMKESQEKFAADEKKLADENKTLTAERNKINEDAQKLQNEQKNVEASIKKK
                     VAALEKDAPRLRSKEIQARQNAIQKEANAFQAKVTAIQKREADFAKKAEAFQKRADEF
                     QKRIEQANKENGGINPQEAQKQAVDEVNATIKEIAKSKGYTLVLQPQVALYAEDEGKD
                     ITEAVLDAIVKKHPEIKIEKPATEAQPATEKETKAEETKPEEAKK"
     gene            6275..7300
                     /gene="lpxD"
                     /locus_tag="PXD71_000117"
     CDS             6275..7300
                     /gene="lpxD"
                     /locus_tag="PXD71_000117"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDL6290286.1"
                     /translation="MANFRLIDLAEHIGGTLRGNADLAIQSIAALDKADSSQITFISN
                     AKYRENLTACNAGAIIVSPADVEFCRADQNLIIVANPYVAYAQLAQYMDSTPKAASEI
                     HPSAVISPEAKLGNNVSVGANAVIESGVELGDDAVIGAGCFVGKNSKIGARTQLWANV
                     SVYHNVQIGADCLIQSSTVIGSDGFGYANDKGQWIKIPQTGGVIIGNRVEIGACTCID
                     RGALDPTVIEDNVIIDNLCQIAHNVHIGFGTAVAGGVIMAGSLKVGRFCQIGGASVIN
                     GHMEICDGAIITGMGMVMRPITEKGIYSSGIPLQTNKEWRKTAALVMNIDEMNKRLKS
                     LEKRFND"
     gene            7329..7787
                     /gene="fabZ"
                     /locus_tag="PXD71_000118"
     CDS             7329..7787
                     /gene="fabZ"
                     /locus_tag="PXD71_000118"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL6290287.1"
                     /translation="MTTENREPNIIEVTEIMDMLPHRYPFLLVDRVTDYEEGKWLKAI
                     KNVTFNEPCFTGHFPGAPIFPGVLILEAMAQATGVLAVATYGKLSEDELYYFAAIDNA
                     RFKRPVVPGDQLQLEVEFLKEVRGITKFTGKAYVDGKLVCEADLMCARRK"
     gene            7821..8615
                     /gene="lpxA"
                     /locus_tag="PXD71_000119"
     CDS             7821..8615
                     /gene="lpxA"
                     /locus_tag="PXD71_000119"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDL6290288.1"
                     /translation="MRLIDSTAKISPLAIIEEGAEIGAHVEIGPFSVIGKDVKIGART
                     KIHSNVVINGMTEIGEDNHIFQFASIGEINQDLKYQGEPTKVVIGNRNRIRESVTIHR
                     GTVQGGGVTKIGDDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
                     IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHARPFGINFEGLKRRGFDKPTMHAIR
                     KVYKLIYSSGKTLEECLVEIEQIAATEPAIAIFKQFFKRSTRGIIR"
     gene            complement(8653..10653)
                     /gene="mnmC"
                     /locus_tag="PXD71_000120"
     CDS             complement(8653..10653)
                     /gene="mnmC"
                     /locus_tag="PXD71_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDL6290289.1"
                     /translation="MGKLTFATLTFNDADTPVSEQFDDIYFSTQDGLAESYYVFQEGN
                     QLWEKWQSHPKEAFVIAETGFGTGLNFLAVADKFQQFRAAFPQSKLKRLYFISFEKFP
                     LTASQLADIHQRYPQFATLSENLTACWQPRQIGCQRYHFEQVYLDLWFGEIAENLPQL
                     GDLYSGTIDAWFLDGFSPDKNPEMWNEQLYLQMFRLSANGGSFATFTASSMVRRGLEA
                     AGFAVKKRKGFGKKREMLWGEKPLDSQVSEPNYPYFYAKNETADDIAIIGGGVASFFV
                     ALSLLEKGKQVTLYCKDETLAINASGNLQGAIYPQLSDDDERNVQFYIHSFDYALQRL
                     QQLKSNIEFEHNLSGVVLYAYDEKTAKKLEKMVEQTADDTLLKSCSQAELSEKLGLSV
                     PNSGAFIPQAGWLSPVQLVRNGFTWLEQKGLKIVLNHKVEHLTFSNGMWQWQTRGETV
                     RHQAVVLANGHTLNEFSQANGIPLYPVRGQVSQIPTTSELQKLKCVLCYDGYLTPMSA
                     YGTHCIGASHIRDNAETEFSLSEHWENVAKLQQNVTVCEWTQGIDLSENKAKVGIRAA
                     FRDRVPMVGAVHHFEQQKSQYANLYNQLRRKQAVENAEIFTNLYMVNGLSSRGLTTAP
                     LLGELLASLIVNEPVPISEDIWHNLSPNRAWIRKLLKGSTVA"
     gene            complement(10878..12314)
                     /gene="trpCF"
                     /locus_tag="PXD71_000121"
     CDS             complement(10878..12314)
                     /gene="trpCF"
                     /locus_tag="PXD71_000121"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDL6290290.1"
                     /translation="MQNIVQDNAQPNQPTILQKIVQDKAIWVAQKEQAFPLSEFQHKI
                     VKSDHDFYAALAKGTHQEPAYILECKKASPSKGLIRAEFDLDSIAQVYKNYAAAISVL
                     TDEQYFQGDFRYIDQVKRQTTQPILCKDFMISAYQVYLARFYNADAILLMLSVVNDET
                     YRELATLAHELGMGVLTETSTEAEFERALALGAKVIGVNNRDLHTLTIDMNRIVRLVE
                     KYQAQISADVCLISESGINDHQQVKNIKPYVHAFLIGSSLMGSADLNNAVRAVIFGEN
                     KVCGLTRVQDVKAVYEQGALYGGLIFAEGSPRQLSLRQAQELVVNAPLRYVGVFQNQA
                     VEFVEKMAKQLELFAVQLHGSEDEQYIAELAEKLGSKTQIWKALSVDVEVENLEFNDN
                     PQIARYVLDSKVGNQQGGTGKTFNWALIPETLKQKALLAGGIGTENIEQALAQGCVGL
                     DLNSGVESAKGVKDLQKLTACFGKIIQA"
     gene            12402..12713
                     /locus_tag="PXD71_000122"
     CDS             12402..12713
                     /locus_tag="PXD71_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDL6290291.1"
                     /translation="MKSQVIQHLSYLEIALRVNQLWEATPPSPEALANEQPFCVETLT
                     PTQWLQWIFIPRMRAILDANADLPRNFAITPYLEEALKNEGYLQAICEPLLGLEKLLK
                     D"
     gene            12867..13595
                     /gene="truC"
                     /locus_tag="PXD71_000123"
     CDS             12867..13595
                     /gene="truC"
                     /locus_tag="PXD71_000123"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDL6290292.1"
                     /translation="MTLDILYQDNSLIAINKPAGVLVHRSWLDKHETVFAMQTLRDQI
                     GQHVFPIHRLDRPTSGVLLFALNSETARIMSEQFEQHLVQKTYLAVVRGYLQGSAQID
                     YPLKVILDKIADKFSQEKEAQAAITDYKGLATVEMPYPVGKHQTSRYSLVELSPKTGR
                     KHQLRRHLKHLFHPIMGDSKYGDLHQNRALVEKSGVSRLMLHAHKLQFQHPKNLQKIE
                     IIASLDEQWQRLFAFFDWNFTQYY"
     gene            13613..13777
                     /locus_tag="PXD71_000124"
     CDS             13613..13777
                     /locus_tag="PXD71_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDL6290293.1"
                     /translation="MDNSLLSLTHEQQQAAVEEIQKLMAQGISAGEAIQIIANQLRDK
                     YQILEKSNNE"
     gene            13770..14246
                     /locus_tag="PXD71_000125"
     CDS             13770..14246
                     /locus_tag="PXD71_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290294.1"
                     /translation="MNKLWTVLALTITSHYSFAAAEKIVTKDLPAEGKPAVVLQVFDE
                     SEKIAKPIKGNKLFISKKQTRLCWHSINLPVQNKVTIAEAFYAPAAMKLASPGSKINS
                     SADKKNHTIITEINLANNQVLNRCWGFDKTDPVGKYKMEIQINDHIFKGLEFEIVK"
     gene            complement(14283..15134)
                     /locus_tag="PXD71_000126"
     CDS             complement(14283..15134)
                     /locus_tag="PXD71_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL6290295.1"
                     /translation="MENQKQAEIELKIMLEQQNVALVENYLNQTSPFPVLAHQTELLG
                     NTYYDTPEQFFATQKMGLRVRSVNQQYEITLKTQGEIVGGLHIRPEYNLALPNSQPDF
                     QTLVEKYQLPFENSEQIAESLQAIFSTDFTRKTWLVQVGMSDIEVALDQGIIKNHFGE
                     EPICELEFEIKQGNLGDLFTLVEAMPKADGMWLSNLSKAQRGYLLGQAVKWEQKIATA
                     MDGENSEKLTQLLADFICVGNESLEVLVRFNQCTQQQFSNWQQARAFVKSKDYLLHNL
                     TNLKTMI"
     gene            15195..16604
                     /locus_tag="PXD71_000127"
     CDS             15195..16604
                     /locus_tag="PXD71_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDL6290296.1"
                     /translation="MFSKIHHKLNNFVQRGLDNHLRLAVTGLSRSGKTAFITSLVDQL
                     LHINKGGNSHLNLFAPARHGQILSVKRVEQGDLTIPRFEYDKNRQSLENQPPQWPEST
                     TGISEIRLAIRYQRQHSLLRHIKETGTLYLDIFDYPGEWLLDLPLLSLSYKEWSQTQQ
                     TIHKDERAELATPWLQAVKKLDLFAKTDENLLAEFSQQYTDYLLACKAQGMQYIQPGR
                     FVLPTENAKGAPVFQFFPLLDLSEAEWEKLEQSDRNSIFHTLKKRYEHYQKKIVKPFY
                     EDYFSQFDRQVILADCLTPLNYGYNEFMEMKLGLQQLFKHFHYGNRSLFHRLFSSNID
                     KLLFIATKADHITSDQVPNLESLMRQLVQEGGRHAEFDGIETGYLAISSIQATDAVKV
                     TQQDKIIKAIRGIRSSDRKQVTLYPGSVPNRIPDKNYWQFNRFEFDQFEPKQLDYSQA
                     IPHFRMDSVLQFLLADMFE"
     gene            16650..17000
                     /locus_tag="PXD71_000128"
     CDS             16650..17000
                     /locus_tag="PXD71_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6290297.1"
                     /translation="MNTVISQRKPIHWVGSTLDDIREFPDNIKRELGFDLDLVQQGLL
                     PRDFKAMANLGSGVMEIRVKDPSGAFRLVYLAKFKDAIYCLHAFQKKTQKTAPKDIAV
                     IKARYAAVKELENE"
     gene            16993..17316
                     /locus_tag="PXD71_000129"
     CDS             16993..17316
                     /locus_tag="PXD71_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL6290298.1"
                     /translation="MNNIDTKIRHTTEAQGNVFEDLGFPIDEAQKLKSASQQLIETKL
                     MLMNEMSNWIDKNNLKQSEAASILGVSRPRISDMVNGKLEKFTLDTLVTFVAKTGKTI
                     QLQLS"
     gene            17424..17903
                     /gene="smpB"
                     /locus_tag="PXD71_000130"
     CDS             17424..17903
                     /gene="smpB"
                     /locus_tag="PXD71_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL6290299.1"
                     /translation="MAKKPKVASNTIALNKRARHEYFIEDEIEAGLELQGWEVKALRA
                     GKGNISDSYVIFRHGEAFLFGAMITPLNMASTHIVSDPTRTRKLLLNKRELDSLFGKV
                     NREGYTIVATSMYWKNAWVKVKIGLAKGKKLHDKREDIKDREWQVAKQRIMKNANRG"
     gene            18106..19413
                     /gene="serS"
                     /locus_tag="PXD71_000131"
     CDS             18106..19413
                     /gene="serS"
                     /locus_tag="PXD71_000131"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL6290300.1"
                     /translation="MIDQNLLRTNLEDVATALKIKRNFILDVERVKTLEEQRKTLQVK
                     TETLQAERNTRSKNIGAAKARGEDISALLAEVDSMGNELDTAKVELDKVQAEIRELLL
                     SVPNLPADEVPLGKDDSENLEVLRWGEPRQFDFEVQDHVTLGENLNGLDFAAGVKLTA
                     SRFVVMKGKLARLHRALSQFMLDLHTEQHGYVETNVPFLVNHDTLYGTGQLPKFGEDL
                     FHTQPLTGQDPNEVQRPFGLIPTAEVPVTNLVRDEIIDEDSLPLRYTAHTPCFRAEAG
                     SYGRDTRGLIRMHQFEKVEMVQIVAPEKSMEALEELTGHAEKVLQLLGLPYRKVLLCT
                     GDMGFGAAKTYDLEVWLPAQNTYREISSCSNMWDFQARRMSARCKAKGDKKTRLVHTL
                     NGSGLAVGRTLVAVLENYQNADGSITVPEVLRPYMGGLEVITA"
     gene            complement(19472..19798)
                     /locus_tag="PXD71_000132"
     CDS             complement(19472..19798)
                     /locus_tag="PXD71_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290301.1"
                     /translation="MVSVDFKNNLLEFIFYGLFSEHTQTNIINGLQFYPKAGVAFRKD
                     LRFRKRAVYQREIMVFYQLDKIDEPKKVTIIAFRLGRMKNPIRLDTVQNRRVVTEIFM
                     NYPEKD"
     gene            complement(19791..20003)
                     /locus_tag="PXD71_000133"
     CDS             complement(19791..20003)
                     /locus_tag="PXD71_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290302.1"
                     /translation="MSIVIVDTDFVVHKEDEEWTTEEIKMIEEHLLEEQSAQYQVKRE
                     VPKQFYADIAERLSRGDLTAWEQLDG"
     gene            20190..22775
                     /gene="leuS"
                     /locus_tag="PXD71_000134"
     CDS             20190..22775
                     /gene="leuS"
                     /locus_tag="PXD71_000134"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL6290303.1"
                     /translation="MQQQYNPSEIEPKVQRYWAENKVFKAVKDVTKEKYYCLSMLPYP
                     SGKLHMGHVRNYTIGDVVSRYQRMIGKNVLQPMGWDAFGLPAEGAAVKNNTAPAKWTY
                     ENIEYMKGQLKMLGFSYDWDREVTTCRPEYYKWEQWFFTELYKKGLVYKKTSTVNWCP
                     NDETVLANEQVHEGCCWRCDTPVEQREIPQWFIKITDYAEELLNQLDNLPQWPDQVKT
                     MQRNWIGRSEGVEITFKIAGSNVELPVYTTRPDTFFGVSYVAIAAAHPLAELAAQNNP
                     ALAEFIREAKNTKVAEAELATMEKKGMATGLFAIHPLTGKEVPVWVANFVLMHYGTGA
                     VMAVPAHDERDFEFAQKYDLQINQVIQPLDGSEWDFSKAAYTEHGKLINSAEFDGLEF
                     DVAFTAIADKLEAMGVGKRQVNFRLRDWGVSRQRYWGAPIPMMTTEEGEVVTVPLQDL
                     PVILPEDVVMNGVQSPIKADPEWAKTTYNGKPALRETDTFDTFMESSWYYARYTCPQY
                     HEGMLDSDEANYWLPVDQYIGGIEHATMHLLYFRFFHKLLRDAGILNSDEPATKLLCQ
                     GMVLADAFYYTSPTNERIWVSPTQVTLERDEKGRIIKATDPQGRELVHSGMTKMSKSK
                     NNGIDPQEMVEKYGADTVRLFMMFASPAEMTLEWQESGVEGAKRFLGRVWNLVYSYAQ
                     NPATTALDVNALDKDQKELRRDVHKTIAKVSDDIGRRQTFNTAIAAVMELMNKLTKAP
                     LETEQDKAVMAEALSAVVRMLYPITPHICFELWQVLGNEQTIDFAPWVQADESAMVED
                     EKLVVVQVNGKVRGKITVSATATEDEVKAIAKADPNVAKFLDGQQIVKEIYVPFKMLS
                     FAVKA"
     gene            22975..23463
                     /locus_tag="PXD71_000135"
     CDS             22975..23463
                     /locus_tag="PXD71_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290304.1"
                     /translation="MIKKLSLIAVFALTACGWHFKNNEVLPESFRTLTFESADQHSEM
                     SRILRNQLQLSDVKLVPSTANVAKLRLVSTSTDSKVVSVFKQAREAEKILTLNVDAIA
                     DIPNKGQYPLEVSVHRTFFDDSRAALAKSAEKEMIMKDMYEQASRQLIIKMAGLKKEL
                     SK"
     gene            23506..24531
                     /gene="holA"
                     /locus_tag="PXD71_000136"
     CDS             23506..24531
                     /gene="holA"
                     /locus_tag="PXD71_000136"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL6290305.1"
                     /translation="MQKIFPETLQFSLNKGLQPFYLLTGNDLLLVNETKDAIIHTARL
                     NGFDEKKEVEIKNDTNWEELFEAAQSMGLFFARQIMVLNFPDSPTATQFKKLAELCAF
                     IHSDLLFILHLPKFSKTMEKQAWFTQIELNLVQINCQTPELSKLPQWVSHRAKAMNLQ
                     IENEANQLLCYSYEGNLLALKQALQLLQLNATDGKITLTKAQEVVEHSAQFTPFQWID
                     ALFEGKIQRAQRILYHLQNEEVQAVVLLRIVQKELMILLEITRNPTAVVSSEQSLFIG
                     NLRTEFDRLKIWQNRRPFYNQITQRLTYQKLYALIQQLAELERKVKQEFSDDIWQELE
                     RFSAQFT"
     gene            complement(24532..24717)
                     /gene="yacG"
                     /locus_tag="PXD71_000137"
     CDS             complement(24532..24717)
                     /gene="yacG"
                     /locus_tag="PXD71_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDL6290306.1"
                     /translation="MPETIVNCPTCEKEVVWSKESKYRPFCSERCQIIDLGDWAAEKH
                     SIASEEAELFSEDLEKY"
     gene            complement(24699..25334)
                     /gene="coaE"
                     /locus_tag="PXD71_000138"
     CDS             complement(24699..25334)
                     /gene="coaE"
                     /locus_tag="PXD71_000138"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL6290307.1"
                     /translation="MTYVVGLTGGIGSGKTTVSDLFSALGVEVIDADVVARQVVEPGT
                     PLLAKIVEHFGEEILTAEKALERTKLRQIVFNNEEEKTWLNNLLHPAIREEMVKKLQE
                     STACYVIWVVPLLIENKLTEYCDRILVVDVSPEIQLERATKRDNSKIETIKNIMAAQV
                     SREERLSYADDVIENNLPFEQGLLLIKEQVQALHYKYLALAKKEKECQKLL"
     gene            complement(25370..26026)
                     /locus_tag="PXD71_000139"
     CDS             complement(25370..26026)
                     /locus_tag="PXD71_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL6290308.1"
                     /translation="MLILFSLFIAVWLKAEIPRFAIRVNRQIYQETTSLVNIGLTEQQ
                     FLAQSVLQPKQPRSANLFFLLFPLIMLCSESPIIGTIFILLCFLSLLDMCYYLTDIRY
                     LVLIFLLVLWESLADFQHQTFTFTLAFCLLIGLVSQLMFKKEAFGSGDMLLLVALSPL
                     FRLEKMLLLILTASLLGIGYYLAYWLMKKQKLAKLAFIPFISTAMLLVLMLESRGINY
                     "
     gene            complement(26029..27225)
                     /locus_tag="PXD71_000140"
     CDS             complement(26029..27225)
                     /locus_tag="PXD71_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL6290309.1"
                     /translation="MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIR
                     ISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQALSMLLQNCRNIRLYLWLNELIQ
                     HIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
                     KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGT
                     VLLGTLLLAVIFYLFFAKKSRLVAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLN
                     AHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGINPNVFGAEMAQMLEV
                     GEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFD
                     MGALVE"
     gene            complement(27222..27674)
                     /locus_tag="PXD71_000141"
     CDS             complement(27222..27674)
                     /locus_tag="PXD71_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HDL6290310.1"
                     /translation="MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAIPLQETEE
                     QLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSDELKFLLNALSPEQSSRQSEIYEEN
                     TAYSLAHSEQVDRNDPIIQILENLFKFCLQHNASDIHFEPQKEKLLRQ"
     gene            complement(27695..28174)
                     /gene="ppdD"
                     /locus_tag="PXD71_000142"
     CDS             complement(27695..28174)
                     /gene="ppdD"
                     /locus_tag="PXD71_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDL6290311.1"
                     /translation="MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNS
                     YTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIA
                     VTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG"
     gene            28355..29734
                     /gene="radA"
                     /locus_tag="PXD71_000143"
     CDS             28355..29734
                     /gene="radA"
                     /locus_tag="PXD71_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL6290312.1"
                     /translation="MAKAPKTAYVCNDCGAEYSRWMGQCKECKAWNTISEVRLISSKE
                     AAKSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGFNELDRVLGGGVVPGSAILIGGH
                     PGAGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANHLGLPTANLKMLSETSVEH
                     ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVG
                     HVTKDGTLAGPKVLEHAIDASLLLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGL
                     KEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHSMLANPRRVAVGLDH
                     NRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQD
                     LVIFGEVGLAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLS
                     DALDILYNL"
     gene            complement(29789..30019)
                     /locus_tag="PXD71_000144"
     CDS             complement(29789..30019)
                     /locus_tag="PXD71_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDL6290313.1"
                     /translation="MDYVEILGYVAMILVACSFLLKDVIKLRVVNSLGCACFVVYGLL
                     IGSIPVTGLNALVVCINLYYISKGNRPTAAHS"
     gene            complement(30139..30633)
                     /gene="folA"
                     /locus_tag="PXD71_000145"
     CDS             complement(30139..30633)
                     /gene="folA"
                     /locus_tag="PXD71_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDL6290314.1"
                     /translation="MKISIIVARTINKVIGKDNQMPWHLPVDLAWFRQNTLGKPVIMG
                     RKTYESIGRLLPKRPNIILSRSGFTVEGAYAVQSLEQAVELAKNFANTAEIMIIGGGE
                     LFKQALPLAERLYLTEIQADIEGDTFFEFDEQNWALVEEKWSEIDENNPFRCRFMCLE
                     RKSV"
     gene            30709..30864
                     /locus_tag="PXD71_000146"
     CDS             30709..30864
                     /locus_tag="PXD71_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl kinase"
                     /protein_id="HDL6290315.1"
                     /translation="MATLFLVTSEQIMSKTIVIKFGTSTLTHGTKSLSRPYMLELVKL
                     NYTNSIV"
     gene            30861..31829
                     /gene="proB"
                     /locus_tag="PXD71_000147"
     CDS             30861..31829
                     /gene="proB"
                     /locus_tag="PXD71_000147"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL6290316.1"
                     /translation="MIVVTSGAAAAGRDYLGHPELPKTLASKQMLAAVGQSQLIRVWE
                     NLFDIYNIHIGQMLLTRADLDDRERFLNARDTLDALLAQKIIPVINENDAVATEEFKV
                     GDNDNLSALVAILAQADQLYLLTDQEGLFSADPRSNPDAKLLSVVEKITPEIRQIAGG
                     SSTGLGTGGMSTKITAADIATRSGVETIIASGSRPNVLVDLANGQNIGSKFLVQSDRL
                     EGRKRWLFAAPQVGEIVVDFGAEQALLQHKSLLPVGVSAIKGEFTRGEVIKIFSQDGK
                     ALALGIARYSSEALLRIKGKKSKEIESLLGYEFGSVAVHSDEMVVY"
     gene            31829..32593
                     /locus_tag="PXD71_000148"
     CDS             31829..32593
                     /locus_tag="PXD71_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDL6290317.1"
                     /translation="MELGIDVLAILFAVATLAGFIDAIAGGGGLLTIPALMAAGLPPA
                     MALGTNKLQACGGSLSSSLYFIRKKAVNLKQIWLLILCTFLGSAAGTIFVQMINVDFL
                     KTLLPFLILIIGIYFLVSPKIGDEDRKQRISFTLFAFTAAVALGFYDGMFGPATGSFM
                     TLAFILLLGFNLSKAVAHAKVINFTSNFASLIFFTLGGAVMWKIGLIMMVGQFIGGTL
                     GAKMVMTKGKKLIRPMLVTMSFMMVGKMLYEQGIFN"
     gene            32596..33228
                     /gene="nth"
                     /locus_tag="PXD71_000149"
     CDS             32596..33228
                     /gene="nth"
                     /locus_tag="PXD71_000149"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL6290318.1"
                     /translation="MNKAKRIEILTRLRNENPKPTTELNYSNPFELLIAVILSAQATD
                     KGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNG
                     EVPEDRDALEALAGVGRKTANVVMNTAFGHPTIAVDTHIFRVSNRTNFAPGKNVVQVE
                     EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEFKEKTE"
     gene            33425..34675
                     /locus_tag="PXD71_000150"
     CDS             33425..34675
                     /locus_tag="PXD71_000150"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HDL6290319.1"
                     /translation="MDIYLVGGAVRDQLLGLPVKDRDFLVVGATAEQLLAQGFQQVGA
                     DFPVFLHPETKEEYALARQERKNGNGYNGFICDFSPNVTLEQDLIRRDLTINAIAQDE
                     NSTLFDPYGGVQDLENRLLRHVSPAFSEDPLRVLRVARFAARFHSFGFTIAPETLKLM
                     REMVQSGELKHLTAERVWLETQKAFETDNPHVYFDILRLIGALKVLFPELDRLFGVPQ
                     PPQHHPEIDSGKHTLMVLQQAKRLAKKAENPTALLFAALCHDLGKGLTPADILPHHYG
                     HEVKGIQPTRELANRLKIPTEIKDFALLVTEYHTHCHKMAELRPETVIKLFNSLDVWR
                     KPNRFFDFLFACEADARGRLGFEQRNYPQPELAKKYYQAAIEIDVQQVIQDGFEKQAI
                     REELNKRRVYAVRSIKLDSDLINH"
     gene            complement(34776..35711)
                     /locus_tag="PXD71_000151"
     CDS             complement(34776..35711)
                     /locus_tag="PXD71_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL6290320.1"
                     /translation="MSESKPFPITTGFFAMPLGLGALGLAWLNATPITPYATTIGNII
                     AGTSVILWVALLGCYVYRVFFCFEQIKAEFMHPVQCCLFSLVTMTTMISGDVLYVWDF
                     YLGELLIFLGVIVHLIFLVFIIGGLWKGTQFLPETLHPVHYLPPIAGSFTMASSLSLI
                     GYSEIASLFLGIGIISWLVYEPTMLQRIRLSPLFPIARPSLGIVLAPAFVGASAYFSM
                     GGELDLLAKMLWGYGFLQFCFILRNLKWIAENGFSMGFWAFSFGLAAMARGAIEFYQF
                     EPLSFIGLAGFTIANFGIGLLLIFTLKLWLQQSRA"
     gene            complement(35840..36034)
                     /gene="iscX"
                     /locus_tag="PXD71_000152"
     CDS             complement(35840..36034)
                     /gene="iscX"
                     /locus_tag="PXD71_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDL6290321.1"
                     /translation="MKWTDTRMIAENLYDMNPDLDPTTVRFTDMHKWICEMEDFDDDP
                     EASNEHILEAILTIWLEEYE"
     gene            complement(36034..36375)
                     /gene="fdx"
                     /locus_tag="PXD71_000153"
     CDS             complement(36034..36375)
                     /gene="fdx"
                     /locus_tag="PXD71_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDL6290322.1"
                     /translation="MPKIVFLPHEEFCPEGMVVEAKAGDNLLEVAHEAGVEIHHACDC
                     SCACTTCHVVIREGFDSLNDTSDQEEDMLDKAWGLEIDSRLSCQCIVGEEDLVVEIPK
                     YNLNHANEAAH"
     gene            complement(36394..36759)
                     /locus_tag="PXD71_000154"
     CDS             complement(36394..36759)
                     /locus_tag="PXD71_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 51 family protein"
                     /protein_id="HDL6290323.1"
                     /translation="MSNYVKTEISEQFRTVAVEFDLEDNRIFELGERINTEYDVAYMN
                     GYNWAAFLRAYLSIYRPSLLELLEEDPDVGCYYVTYPLSEKSKEKARELKKIIVYLVE
                     NEEEILNFIGEHLDNIEWD"
     gene            complement(36769..>38601)
                     /gene="hscA"
                     /locus_tag="PXD71_000155"
     CDS             complement(36769..>38601)
                     /gene="hscA"
                     /locus_tag="PXD71_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDL6290324.1"
                     /translation="EPGQTAAPHQHKLAVGIDLGTTNSLVATVRSGQTQVLLDEQERA
                     LVPSVVHYAETEKTVGIEAFAKAALDPKNTVISAKRLIGRSLADVQQRYQNLPYQFVA
                     SENGLPLIQTAQGNKSPIEVSADILSHLAQFAEKRLAGELSGVVITVPAYFDDAQRQS
                     TKDAARLAGLNVLRLLNEPTAAAIAYGLESGQEGVIAVYDLGGGTFDLSILRLRKGVF
                     EVLATAGDTALGGDDFDHLLAEWIAEQANYKPQTASEQRELLTLATQTKVALSQAVEF
                     EVKFANWTGVVSRSQFNELIQPLVKRSLMTCRRALKDAGVEAEEVREVVMVGGSTRVP
                     FVREQVGEFFGKTPLTSIDPDKVVAIGAAVQADILVGNKPDSDMLLLDVVPLSLGIET
                     MGGLVEKIIPRNTTIPVARAQEFTTGKDGQTAMTIHVLQGERELVEDCRSLGKITLRG
                     IPAMAAGVAQVRVTYQVDADGLLNVTAMEKSTKVQASIQIKPSYGLTDEEVTQMIKSS
                     MANAKEDMEARQLVEQRVEADRVIDSVVTALKEDGSAVLSVEEFQAIEAQLARLIELK
                     EGTDRFAIQQGIKEVDLATQEFAARRMNLSIQKALAGKKMDDLA"
BASE COUNT        11790 a         8186 c         7625 g        11000 t
ORIGIN      
        1 ctcaaaatat gcctaagcac gttgccatta ttatggatgg caatggcaga tgggctaagc
       61 agaaaggaaa attacgcatt tttggtcatc agaatggtgt aaaggcagtt cgttctgcag
      121 ttagttttgc agcaaagcat catatccaag ttttaaccct ctacgcattt agcagtgaga
      181 attggagcag accagaaact gaagtttcag ctttaatgtc tctgtttatg aaagctttag
      241 attctgaagt taaacaatta cataaaaata atattcgctt aaagattata ggcgataaat
      301 ccgcctttag cgaaaattta caaaaacgca ttgccgaatc ggaaaaatta accgaaaaca
      361 atactgggct aatcttaaac attgctgcca actatggtgg ttactgggat attgttcaag
      421 ctgctcaaca actagcagtg cacgttaaat ctgaacaact tgctgtggag cagattacac
      481 cagcattgtt tcaacaacat ttagtgacag aaaaccagcc gcaagtggat ttattaattc
      541 gaaccagtgg tgagcagcgg atcagcaatt ttctattatg gcaaattgct tatgccgagc
      601 tgttttttag cccggtttta tggcctgatt ttaatgatga ggcttttagt gatgcaattt
      661 atgcttatca gcaacgtgaa cgccgattcg gtggttgtta atataaaaag aataaggaaa
      721 tacaatgctt aaagaacgtg tactttcagc gattattatg attattgccg tgctggctgc
      781 catattttgg ctctcaccac ttcctttaac tttagtgtta tcggcaattg ttgtggctgc
      841 aatgtgggaa tgggctcaat ttgccggctt taaacaccct gtgccacgtg caattgtggc
      901 aatggtgaca atctgcttat tactttttgc gatcgttgcg aatactgatt atatccgagc
      961 cacccgtttt attactgatg agactacccc tatcctcttt attggctgta tttggtggtt
     1021 aattgccctt gtcctagtgg caacttaccc caactcggca aaactgtggg aaaaatctgt
     1081 tgcagctaaa ttcctatttg gacttgctac cttagtgccg tttttaattg gcacgctcgc
     1141 attgcgtttc cataattatg catttgatcc ataccaagga acttatttat tcctttacgt
     1201 ctgtttatta gtttggggag ctgattcagg tgcttatttt gctggcagag cctttggcaa
     1261 aagaaaactt gccccaaaag tatctccggg taaatcgtgg gaaggcgtaa ttggtggcgt
     1321 actcacctct agtttgattg cctttacctt tttacaactt acaccggcaa atgtattcgg
     1381 caaagcatta gccactaccc catttatttt agtttcggtc ggtatggttg ctatttcagt
     1441 cttaggcgat ttggcagaaa gtatgtttaa acgccaagca ggcattaaag acagtagcca
     1501 gttaattcct gggcatggag gtattttaga tcgtattgat agtttaaccg ctgccgttcc
     1561 gttttttgct acattcttct tttttgtact atagaatatg acgtccatca ttgcattttt
     1621 tattttaatt tgcgtactgg tttttgtgca tgaatacggg cacttttggg ctgctcgcaa
     1681 atgcggggta aaggtgatcc gtttttcgat cggatttggt aaagtcttat ttaagaaaaa
     1741 agataaacaa ggaaccgaat ttgttttttc acttattcct ctgggcggct atgtacaaat
     1801 gtggaatgat gaaacggata tcaacgctcc agctcaacaa gctcttaaca caaaatctgt
     1861 attgcaacgt gcatttatta ttatagccgg tcctgctgca aattttattt ttgcgatttt
     1921 ggcatactgg gtcgttttta ttgccggtat tccaactgtt aaacctgtta ttggggaaat
     1981 tttaccaaac actatcgcct cccaagcaag aattcctacc gaattggaaa ttacaaaaat
     2041 cggtcatcaa tctatccaag attgggaaag tgcatcttta gcactagttg gttttgtggg
     2101 caataaaaat gtaccgttag agggaaatat caacggcaac tcaacacaaa aatatgaact
     2161 cgatttatct aactggagta ttgactcagc caaagaaaac ccattaaccg cattaggtat
     2221 tcgccccaaa agtgcaaaag tcaccgctga aattaaacaa gttgtcgaaa attctcctgc
     2281 aagccaagtg ggtattcaag caggtgatcg tattcttcaa attaaccagc agccatttaa
     2341 ttggttcaat ttagttgaac tggtacaagc aggtaaacct attgaactta aaattgaaca
     2401 acgaggtcag attaaagacc ttgtcgtcca accagaaaaa aaagacgaac gctatattat
     2461 cggggtaata ccaagttatg agcctcttgc agataaatat cgtaccgaat taaaatatga
     2521 tattcttacc gcattttata aaagtatcga aaaagtgtgg tctcttaccc aaaccatact
     2581 ccaatttatc ggtaatttaa taagcggtga tttgtccatt aaaaatttag gtgggccaat
     2641 ttcaatggca aaaggagccg gtgcaacagc tgaaatcggc ttagtttatt accttagttt
     2701 tatggctcta atcagtgtca atttgggggt aatgaactta tttccacttt tacccttaga
     2761 tggtgggcaa cttgttttat tggctgttga agcaattcgt ggaaaagcat tatcagaaaa
     2821 aatacaactc aaattccaac aaattggatt tgcattcgtg ctgagtttaa tgttattcgc
     2881 ctttgccaac gatataattc acttttaaca agcggttaaa aaaggtgact tttttgcaaa
     2941 aagcaccctg acatcaaaat ccataatatg gatttaaact atattcttaa taattttgca
     3001 aaacgatcat aggataattt caatgaaaaa attattactt tcctctcttt taattgcaaa
     3061 cggtgtagtt gctgcacctt tcgtagtaaa agacattcgt gttgaaggtg tgcaacctac
     3121 aacaggcgaa tctatcattt catctattcc ggttagagta ggtcaaacag caacggatac
     3181 tgacgtatct aatgtcgtcc gccatctatt cagccaacaa cgttttgcag atgttcgtgc
     3241 aacacgagaa ggcaatacgc ttgtcatcaa agtcgctgaa aaaccgatta ttggcaaggt
     3301 agagatcgaa ggtaaccaag cgattcctaa agatgcctta gagcaaaatt taaaggcaaa
     3361 cttaattaac caaggtgaaa tttttgatgc tgccaagctt gaagctttta aagaaggact
     3421 aatcgaacat tatcactcta ccggccgtta tgaagccaaa attgacacat ctgtttcgaa
     3481 taacaccgaa ggtggcgtta atattaagtt agcgattaat gaaggtgaag ttgctaaagc
     3541 caaaacaatt aagtttgaag gtaataatgc cttctctgat aaaaaattat tagaaaactt
     3601 agatattcaa cctgatgttt cttggtggaa tatttttgaa agtagcaaat tcgaacaaac
     3661 cgcctatcaa aaagacttag aaacattaag agatttttat ttaaatcgtg gctatgcaaa
     3721 atttgctatc caagataccg atgttcaatt caatgataat aaaacggaag ttgcattgac
     3781 ctataaatta gatgaagggt cacagtacca tgtcagtgaa atgcgcatta tcggtaatac
     3841 agccaatatg gatagccagc tcaacgccct actaaaagaa ttcaagcctg gtcaattatt
     3901 ccgtaaatca gaccttacac aaattgaaga agagattaaa tctattttag gggacgcagg
     3961 ttatggttca gctaaagtag atatttatcc tcaatttaat gaagaaaata aaaccgtaaa
     4021 aatcagtttt gttgtggatg ccggtcgtcg tatttatgtt cgtaaaatcc gttttgaagg
     4081 taatggtgtc actgcagatt caacattacg tcgtgaaatg cgccaacaag aaggtgcttg
     4141 gctttccaca aacaaagctt ctctaggtaa agctcgttta gaaagaaccg gtttctatga
     4201 aagtgttgat atgtcaatgc caaatgtaga aggtacagcc gatcaagttg atgtagtgta
     4261 taaaattaaa gaacgcaata ccggtagtat taactttggt atcgggtacg gcacgaaaaa
     4321 cggtatcagc tatcaagcag gaattaaaca agataatttc ttaggtatgg ggtcaactat
     4381 cagtctaaat ggtacccgta ataactacgg taccagcgtt aacctaggct ataccgaacc
     4441 ttactttact aaagatggtg taagtttagg cggtaatatt ttctatgaag attatgataa
     4501 ctcagacaat gattcaatgg cttcatataa acgccaaacc tatggtatta acggcacatt
     4561 aggcttcccg gtaaatgaaa ataactcata ctacttaggt ttaggctata cgcacgataa
     4621 aattaaaaat gcagcacgtg agtacactcg tgagaaatat gtaaaatcaa tgaactttga
     4681 gttcgatcca gctgccgatt attacaaaaa aatcaaagct gatgattttg atttttctct
     4741 cggttggaac tacaacagct tgaatagagg ctatttcccg actgaaggaa ctaccgcaaa
     4801 cattggcggt aaaatcacaa ttccaggctc tgataataaa tattaccgag tcagtgcaga
     4861 ctttagaaat tattacccgt taaaccgtga gcacaaatgg gtaatttcaa cgaaagctag
     4921 cattgcttat actaatggat ttggtggtaa agaagttccg ttctatcagc tttatactgc
     4981 cggaggtatt ggtacactaa gaggtttctc ttacggtgca gtagggcctc aagcgattta
     5041 ctataataca aaatccggca aatttgatag catgaataat gatattatcg gtggtaatgc
     5101 gttagctgcg gcaagcttgg aattaatcac accaacgcca tttgtgagcg aaaaatatca
     5161 gcacaatgta agaacctctc tctttgttga tgctgcaagt gcatggaata caaaatggaa
     5221 gaaagatgaa tatacgaact tgcctgacta tggtgatttc aaacgtttcc gtgcttccgc
     5281 cggtattgca ttccaatgga tgtctccaat cgggccatta tcattctctt atgcgaaacc
     5341 aattcgtaaa tatgataatg atgatattga acaattccag ttcaacattg gtagttcatt
     5401 ctaattaacc acaagcggtt aataaatgta aaaaatttgc aacttagccg cttatttcaa
     5461 aactgttcat acaaggaaaa caaatgaaaa aattatttaa aatgactggc ttagccgtag
     5521 ctttagcctc tacaaccgga atcgcgaatg ctgcagatac tattggtttc gtagatccaa
     5581 gctatgtatt acaaaaccac cctgtattat tagatgcttc ggcaaaattt gataaattca
     5641 tgaaagaaag ccaagaaaaa tttgctgctg atgaaaaaaa attagcagat gaaaataaaa
     5701 ccttaacggc tgagcgcaat aaaattaatg aagatgcgca aaaattacaa aatgaacaaa
     5761 aaaatgtaga agcttctatc aagaaaaaag tggcagcatt agagaaagat gcgcctcgct
     5821 tacgttcaaa agagattcaa gcacgtcaaa atgcgattca aaaagaagca aacgcattcc
     5881 aagctaaagt aacagcaatt caaaaacgtg aagctgattt tgcaaaaaaa gcagaagcct
     5941 tccagaaacg tgcagatgaa ttccaaaaac gtattgaaca agcgaataaa gaaaatggtg
     6001 gcattaaccc acaagaagcg caaaaacaag cggttgatga agtaaatgca accattaaag
     6061 agatcgcaaa atcgaaaggt tacactttag tattacaacc acaagtagca ctttatgctg
     6121 aagatgaagg taaagatatt actgaagcag tacttgatgc tattgttaaa aaacatccgg
     6181 aaattaaaat agaaaaacct gcaactgaag ctcaaccagc tacagaaaaa gaaaccaaag
     6241 ctgaagaaac taaaccagaa gaagcgaaaa aataatggca aatttccgtt taattgattt
     6301 agcggagcat atcggcggta ctcttagggg taacgccgat ttggctattc aaagtattgc
     6361 tgctttagat aaagccgaca gctcgcaaat tacctttatc tcaaatgcaa aatatcggga
     6421 aaatttgacc gcttgcaatg ctggagctat tatcgtcagc ccggcagatg tagaattttg
     6481 ccgagcagat caaaatctta tcatcgttgc caatccgtat gttgcttatg ctcaactggc
     6541 tcaatatatg gattctactc cgaaagcagc gagcgaaatc cacccaagtg cggtgatttc
     6601 acctgaagct aaactgggta ataacgtatc ggtaggggca aatgccgtga ttgaaagtgg
     6661 cgtagaattg ggcgatgatg ccgtgatcgg tgcaggctgt tttgtgggaa aaaacagtaa
     6721 aatcggggca agaacccaac tttgggcaaa tgtttcggtg taccacaatg tacaaatcgg
     6781 agctgattgc ttgattcaat cctcaacggt tatcgggagt gatgggtttg gctatgcgaa
     6841 tgacaaaggg caatggatta aaatcccaca aaccggtggt gtgattatcg gtaatcgtgt
     6901 tgaaattggt gcttgcactt gtattgaccg aggtgcatta gatccaaccg taatcgaaga
     6961 taatgtgatt attgataacc tttgccaaat cgcccataat gtacacattg gttttggcac
     7021 tgccgttgcc ggcggcgtca ttatggcagg cagtctaaaa gttggtcgct tttgccaaat
     7081 tggtggagca agtgttatca acggtcatat ggaaatctgt gatggtgcca taattaccgg
     7141 aatgggtatg gttatgcgcc caattacaga aaaaggaatt tactcatcag gtattccatt
     7201 acaaaccaat aaagaatggc gaaaaaccgc agcacttgta atgaatattg atgaaatgaa
     7261 caaacgctta aaatcgttag aaaaacgttt taacgattaa ttttattaga ggtcttaaag
     7321 tgacagaaat gacaactgaa aatcgtgagc caaatattat tgaagtaacc gaaattatgg
     7381 atatgttacc gcatcgctac cctttcctgt tagttgatcg cgtaaccgat tatgaagaag
     7441 ggaaatggtt aaaagccatt aaaaatgtaa ccttcaatga accttgtttt accgggcatt
     7501 ttcctggcgc accaatcttc ccaggagtat tgattctgga agcaatggct caagcaactg
     7561 gcgtattagc cgttgccact tatggtaagt taagtgaaga cgaactctac tattttgccg
     7621 caattgacaa tgctcgcttt aagcgaccgg ttgtgccggg cgatcaactt caacttgagg
     7681 ttgaatttct taaagaagta cgtggtatta ctaaatttac cggtaaagca tacgttgatg
     7741 gcaaattagt ctgtgaagca gatttaatgt gcgctcgccg taaataattc gatataagcc
     7801 caaactagag ggaacaaact atgcgtctaa ttgattcaac agcaaaaatt agcccgcttg
     7861 ccatcattga agaaggtgct gaaattggcg ctcacgttga aattggtcca ttttcagtca
     7921 tcggtaaaga tgtcaaaatc ggggctagaa cgaaaattca ctcaaatgtt gtcattaacg
     7981 gcatgactga aattggtgaa gataaccata ttttccaatt tgccagcatt ggtgaaatca
     8041 accaagatct aaaataccaa ggtgaaccaa ccaaagtggt gatcggtaat cgtaaccgta
     8101 ttcgtgaaag tgttactatt caccgaggta cggtacaagg cggtggcgtg acgaaaatcg
     8161 gtgatgataa cttatttatg atcaacaccc acatcgcaca cgactgctct atcggtaacc
     8221 gttgtattat cgccaataat ggtacgctag caggtcacgt taccctagat gatttcgtga
     8281 ttgtgggagg aatgtccgcc attcaccaat tcgttgtgat tggttcacac gttatgcttg
     8341 gaggcggttc aatggtcagc caagacgtac cgccttatgt catggcacaa ggtaaccacg
     8401 cacgcccatt tggtattaac tttgaagggt taaaacgtcg tggctttgat aaaccaacta
     8461 tgcacgctat tcgcaaagtc tataaactga tttactccag cggaaaaaca ttggaagagt
     8521 gcctagtaga aattgagcaa attgcagcaa cagaacctgc gattgctatt tttaaacaat
     8581 tcttcaaacg ttctacccgt ggtattattc gttaataaac gaaaccccga aatcaattcg
     8641 gggtttgttt tatcaagcga ccgtcgaacc tttcaacaat tttcgaatcc aagctcgatt
     8701 tgggctaagg ttatgccaaa tatcctcact gattggcaca ggctcattca caatcaggct
     8761 tgctaacaat tcccctagaa gtggtgcagt cgtcaaacct cgagaagaca ggccgtttac
     8821 catatacaga ttggtaaaaa tttcagcatt ttctaccgct tgtttccgtc tcaactggtt
     8881 gtataaattg gcatattggc ttttttgctg ctcaaaatga tgcactgccc caaccatcgg
     8941 tacacgatca cgaaacgccg cacgaatacc gactttggct ttattttcag ataaatcaat
     9001 tccttgcgtc cactcacaaa cggttacatt ttgctgaagt tttgcaacat tctcccaatg
     9061 ctccgataaa ctaaactcgg tttctgcatt atcccgaata tggcttgctc caatgcaatg
     9121 cgtgccatag gctgacattg gcgttaaata accatcataa caaagcacac acttcagttt
     9181 ttgtaattcg cttgtagtcg gaatttggct cacttgccct cgtaccggat acaacggaat
     9241 gccgttcgct tggctaaatt cattcaacgt atgtccattt gccagcacaa ccgcttgatg
     9301 tcgaaccgtt tctcccctcg tctgccattg ccacattcca ttagaaaagg ttaaatgctc
     9361 aactttgtga ttcagtacaa tcttcaaccc tttctgctcc agccaagtaa agccatttct
     9421 aacaagttgt accggtgaaa gccaaccagc ttgcggaata aatgccccgc tgtttggcac
     9481 gcttaaacct aatttttcac ttaattctgc ctgtgaacaa gattttaata aggtgtcatc
     9541 agccgtctgc tccaccattt tttccagttt tttagccgtt ttctcgtcat aggcgtaaag
     9601 cactaccccg cttaaattat gctcaaactc aatgttggat ttcagttgtt gcaagcgttg
     9661 caatgcataa tcaaaactat ggatataaaa ctgtacattt cgctcgtcat catcgcttaa
     9721 ctgtgggtaa atcgcccctt gtaaatttcc tgaagcattt attgccaagg tttcatcttt
     9781 gcaatacagc gtgacttgct tgcctttttc taacagcgat aacgcaacga agaaactcgc
     9841 tactccgcca ccaataattg caatgtcatc agcggtttca tttttggcat aaaaataggg
     9901 gtaattaggc tcggaaactt ggctatctaa cggtttctcg ccccaaagca tttctcgttt
     9961 tttgccaaat cctttgcgtt ttttcactgc aaaacctgcg gcttccagcc ctcgtctgac
    10021 catacttgaa gcggtaaaag tagcaaaact gccgccattg gcacttagtc gaaacatttg
    10081 cagatataac tgttcgttcc acatttctgg gtttttatca ggcgaaaagc catctaaaaa
    10141 ccaagcgtca atcgtgccgc tataaagatc gcctaattgg ggtaagtttt cagcaatctc
    10201 accgaaccat aaatctaaat aaacctgctc aaaatgataa cgttggcaac ctatttggcg
    10261 aggctgccaa caagcggtta aattttcaga taatgttgca aattgcggat agcgttggtg
    10321 aatatcagct aattggctgg ccgtcagagg aaatttctca aatgagataa aatagagtcg
    10381 ttttagttta ctttgcggaa atgctgcccg aaactgttgg aatttgtcag caacggctaa
    10441 aaaattcaag cccgtgccaa agccggtttc cgcaataaca aaagcctctt tcgggtgaga
    10501 ttgccacttt tcccacagtt ggttaccttc ttgaaacaca tagtaacttt ccgccaaacc
    10561 atcttgggta gaaaaataaa tatcatcaaa ttgctccgac accggtgtat ctgcatcatt
    10621 aaatgtaagt gtcgcaaaag tcagtttacc catcatatcc ccttaaaaac aaaacacgga
    10681 aaaccccaag attttccgtg tttcaaccag ttcaatagta ccaatgttag aaaatgttgc
    10741 tggcaaaaat aacgatcata ccaagtgcgc ctacaaccgt tagcattgaa taaccaaaag
    10801 tacccatttt acaatcctca ttgttaatta tgattaaaac ggctttattc tagcataatt
    10861 tccatcgctt tgaatgccta tgcttgaata attttgccaa aacaagcggt caatttttgt
    10921 aaatctttta cgcctttggc actttccacg ccagaattta aatctaaccc gacacagcct
    10981 tgagcaaggg cttgctcaat attctcagtg ccaataccac ctgccaataa cgctttttgt
    11041 tttaaggttt ctggaatcaa cgcccaatta aaggttttgc ctgtgccgcc ttgctgattg
    11101 cctactttgc tgtctaatac ataacgagca atctgtggat tatcgttaaa ttctagattt
    11161 tcaacctcaa catcaaccga taaggctttc cagatttgag ttttactacc taatttctct
    11221 gccagttctg caatgtattg ctcatcttcc gagccgtgca attgaactgc aaataactca
    11281 agctgttttg ccattttttc cacaaattcg accgcttgat tttggaacac ccccacataa
    11341 cgcaacggtg cattcaccac caattcttgg gcttggcgaa gtgaaagctg gcgaggcgag
    11401 ccttcggcaa aaattaaacc gccatataat gccccttgct cataaaccgc tttcacatct
    11461 tgcacacggg ttaagccaca cactttgttt tcaccaaaaa tcaccgcccg caccgcatta
    11521 ttgagatcgg cacttcccat taaactgctg ccgattaaaa aagcatgaac atacggctta
    11581 atgtttttca cttgctggtg atcgttaata cccgattctg aaatcaaaca gacatcagcc
    11641 gaaatttggg cttggtattt ttccaccaaa cgcacaatgc ggttcatatc aatcgtgagc
    11701 gtgtgcaaat cacggttatt aacgccaatt accttcgccc ctaaggctaa ggcacgttca
    11761 aattccgcct ccgtgctggt ttcagtcaaa acgcccattc ctaactcgtg agccagtgtg
    11821 gcaagttcac ggtaggtttc atcatttacc accgataaca tcaataaaat cgcatcagca
    11881 ttataaaaac gggcaaggta aacctggtat gccgaaatca taaaatcttt acacaaaatc
    11941 ggctgtgtgg tttggcgttt gacttgatcg atataacgga aatcgccttg aaaatattgc
    12001 tcatcggtta aaaccgaaat cgccgcagcg taatttttat acacttgggc aatactgtct
    12061 aaatcgaact cagcacgaat taaccctttt gacggtgaag cttttttaca ttctaaaata
    12121 taagctggtt cttggtgtgt gcctttggca agagccgcat agaaatcatg gtcgcttttt
    12181 acaattttgt gctgaaattc acttaacgga aaggcttgct ctttttgtgc cacccaaatg
    12241 gctttatctt gtacgatctt ttgtaaaatc gtcggttggt ttggttgagc gttatcttga
    12301 acgatgtttt gcatataaat tcctagagcg aaatcggttt gaatggtaaa atctgccctt
    12361 atttttaccc gaaaaagtct catttcacaa gcaaaactaa gatgaaatca caagttatac
    12421 agcatttatc ttatttagaa atcgccctga gagttaatca actttgggag gcgacaccac
    12481 caagccctga agccttagcc aatgagcaac ctttctgcgt ggaaacctta accccaaccc
    12541 aatggctaca atggattttt attcccagaa tgcgggccat tttagatgca aatgccgatt
    12601 tacctcgcaa ttttgcaatc accccttatt tagaagaggc attgaaaaac gaaggttatt
    12661 tacaagcgat ttgtgaaccg ctattaggtt tggaaaagtt gttgaaagat taacttctct
    12721 ctcagttctt ttattaatct gacaaattcg ttgatagtgc cagcgtccac gctggtacta
    12781 attttaacgg ttaattaatc aaaatttggc acaagcgagg acgcttgcgc catcatagag
    12841 atttgataaa ttatacttaa tgtattatga ccttagatat tttataccaa gacaactcgc
    12901 ttatcgccat caacaaacct gccggagtgt tggtgcatcg ttcttggtta gataaacacg
    12961 aaacggtgtt tgcaatgcaa accctgaggg atcaaatcgg gcaacacgtt tttccgattc
    13021 accgccttga ccgcccaacc tcaggtgtat tgctgtttgc tctcaacagc gaaaccgccc
    13081 gaataatgag cgagcagttt gaacaacacc tagtacaaaa aacttatttg gcggtggtgc
    13141 gtggctattt acaaggttcg gcacaaattg attatccgct caaagtgatt ttagataaaa
    13201 ttgcggataa attttctcag gaaaaagagg ctcaagcggc gattactgat tataaaggct
    13261 tagcaacggt tgaaatgcct taccctgtcg gcaagcacca aacctctcgt tatagtttgg
    13321 ttgaacttag ccctaaaacc gggcggaaac atcagcttcg ccgccacctg aaacacctgt
    13381 tccacccgat tatgggagac agcaaatatg gcgacttaca ccaaaaccgt gctttagttg
    13441 aaaaatcagg cgtaagtcgt ttaatgttac acgcccataa attgcaattt cagcacccga
    13501 aaaatttgca aaaaatagag ataattgcat cgcttgatga gcaatggcaa cgcctctttg
    13561 cattttttga ctggaacttt actcaatatt attagacaaa aaagaggatt gaatggataa
    13621 ttcactttta tcattgacac acgagcaaca acaagccgca gtagaagaaa ttcaaaaact
    13681 gatggcacaa ggcatcagtg ccggagaggc aattcaaatt attgctaatc aattacgtga
    13741 caaatatcaa atattggaga aatcaaacaa tgaataaatt atggacagtg cttgccctaa
    13801 caataacttc ccactatagc tttgctgccg ctgaaaaaat agtcactaaa gatttaccgg
    13861 cagaaggaaa acctgcagtc gtgctacagg tttttgatga aagtgaaaaa attgcaaaac
    13921 ctattaaggg aaataagctc tttattagca aaaaacaaac aaggctttgc tggcacagta
    13981 ttaatcttcc tgtacaaaat aaagttacta ttgcagaagc tttctatgcc cctgctgcaa
    14041 tgaaattagc ttctcccggc tctaaaatta attcttctgc agacaagaaa aatcatacta
    14101 tcattactga aataaacctt gcaaataatc aagtattaaa tcgttgctgg ggttttgata
    14161 aaactgaccc tgtcggtaaa tacaaaatgg aaattcaaat taacgatcac attttcaaag
    14221 gtttagaatt tgaaattgta aaataaccta aaagccacag agtaacaccc tgtggctttt
    14281 tgctaaatca ttgtttttaa attggttaaa ttgtgcagca aataatcttt actcttcaca
    14341 aaggctctcg cctgttgcca gttgctaaat tgttgctgag tgcattgatt aaaacgtacc
    14401 agcacttcca aactttcatt gccgacacaa ataaaatcag ctaataattg ggttaatttt
    14461 tcactgtttt caccatccat tgcagttgca attttctgct cccatttaac cgcttgtccg
    14521 agcaaataac cccgctgggc tttgcttaaa ttactcaacc acatcccatc cgcttttggc
    14581 attgcctcaa ccaaagtaaa taagtcgcct agatttcctt gcttgatttc aaactccaat
    14641 tcacaaatcg gctcttcacc aaaatgattt ttaatgatgc cctgatctaa cgctacctca
    14701 atgtcagaca tccccacttg caccaaccaa gttttacgag tgaaatcggt gctgaatatc
    14761 gcttgcaagc tttctgcaat ctgctctgaa ttttcaaacg gcaactggta tttttccact
    14821 aatgtttgaa aatccggctg gctattgggt aaagctaaat tatattccgg acgaatatgc
    14881 aaaccgccta caatttcccc ttgtgttttt aacgtgattt catattgttg attcacagaa
    14941 cgcacccgca gccccatttt ttgcgtggca aaaaattgct cgggcgtatc ataataggtg
    15001 ttgcctaaca attccgtttg atgagcaagc acagggaatg gcgaggtttg attcaagtag
    15061 ttttccacta acgccacatt ctgctgttcc aacattattt tcagttcgat ttctgcttgt
    15121 ttttggtttt ccatttcaca cgcccctgca acgttgtata atcaagcggt tattttatca
    15181 ataaattttg caaaatgttt tcaaaaattc accataaact gaataacttt gtccagcgtg
    15241 gactcgacaa ccatttacgc ctcgccgtta ccggcttaag ccgtagtggc aaaacggcat
    15301 ttattaccag cttggttgat cagcttttgc atatcaacaa aggcggtaat tcgcatttaa
    15361 acctgtttgc gcctgcacgc cacgggcaaa ttctatcggt aaaacgcgtt gagcaagggg
    15421 atttaactat tccccgcttt gaatatgata aaaaccgcca aagccttgaa aatcaaccac
    15481 ctcaatggcc tgaatccacc actggcatta gcgaaattcg ccttgcgatt cgctaccagc
    15541 ggcaacattc tctgctacgt catattaaag aaaccggcac gctttatttg gatattttcg
    15601 attacccggg cgaatggctg ttagatttac ccctgctctc cctttcctat aaagaatggt
    15661 cgcaaacgca acaaaccata cataaagatg agcgtgcaga gcttgccaca ccttggttac
    15721 aagcggttaa aaaactcgat ctttttgcaa aaaccgatga aaatttactg gcagaattca
    15781 gccaacaata caccgattat ttgctcgcct gcaaagctca aggaatgcaa tatattcagc
    15841 cggggcgatt tgtgttgcca actgaaaatg ccaaaggtgc accggtattc caattttttc
    15901 cgttattaga tttaagcgaa gctgagtggg aaaagttgga acaaagcgac agaaacagta
    15961 ttttccatac cctcaaaaaa cgctatgagc attaccagaa aaaaatcgtc aaaccgtttt
    16021 atgaagatta cttctctcaa tttgaccgcc aagtgatttt ggcagactgc ctcacaccgt
    16081 taaattatgg ctataacgaa tttatggaaa tgaaattagg gttacagcaa ctgtttaaac
    16141 atttccatta tggcaatcgt tcgctatttc accgtctgtt ttcgtccaac attgataaat
    16201 tgctatttat cgcaacaaag gcagatcaca ttactagcga ccaagtgccg aatttggaaa
    16261 gcctaatgcg acaattagtg caagaagggg gaagacacgc agaatttgat ggtatcgaaa
    16321 ccggctattt agccatttcc tcgattcaag caaccgatgc ggtgaaagtg actcagcaag
    16381 ataaaatcat taaagcgata agagggatcc gttccagcga tagaaaacaa gtgacactct
    16441 accctggcag cgtgccaaat cgcatacccg ataaaaatta ctggcaattt aaccgttttg
    16501 aattcgacca attcgagccc aagcaacttg attatagcca agcgattcca cacttcagaa
    16561 tggattcggt gctacaattt ttattggcgg atatgtttga gtaaaatctt gttgcatatt
    16621 tccattaaag gtataaaatt ttatacatta tgaatactgt aatttcccaa cgaaaaccaa
    16681 ttcactgggt tggatcaacc ttagatgata ttcgtgaatt tccggataac attaaaagag
    16741 aactcggttt tgatcttgat ttagttcagc aaggcttatt accgagggat tttaaagcga
    16801 tggcaaattt gggttcaggc gtaatggaaa ttcgagtaaa agatccctca ggggcttttc
    16861 gcttagtgta tttagctaaa tttaaagatg caatttactg tttacacgct ttccaaaaga
    16921 aaacgcaaaa aacagcccct aaagacattg ctgtcattaa agcacgctat gctgccgtaa
    16981 aggagttaga aaatgaataa tattgatact aaaattcgcc atactacaga agctcagggt
    17041 aatgtttttg aagatcttgg tttccctatt gatgaagctc aaaaattgaa atctgccagt
    17101 cagcaactga ttgaaactaa actgatgcta atgaatgaaa tgagcaactg gattgataaa
    17161 aataatctca aacaatcaga agcggcttct attctaggtg ttagccgccc tcgaatttct
    17221 gatatggtca atggcaagtt agaaaagttt accctagata ccttagtcac ttttgttgca
    17281 aaaaccggca aaacaatcca attgcaatta tcctgatatt tgcaaaattt tactcaaatt
    17341 caaccgcttg ttgcttttat cattcactaa caaggttatt tcaagtaaaa ttgtgactaa
    17401 ttttttatta ataaacacaa attatggcaa aaaaacctaa agttgcatca aatacgattg
    17461 cgttaaataa acgtgccaga cacgaatatt tcattgaaga tgaaattgaa gccggcttag
    17521 agctacaggg ctgggaagtc aaagcattgc gtgccggaaa aggcaatatc agcgatagct
    17581 atgtcatctt tcgccacggc gaagccttct tattcggggc aatgattacg cctttgaata
    17641 tggcatcaac ccatattgtc agcgacccga cccgcacccg caaacttcta ctgaataaac
    17701 gtgagttaga ctcactgttc ggtaaagtta atcgtgaagg ttataccatt gttgccacct
    17761 caatgtattg gaaaaatgcg tgggtgaaag taaaaatcgg cttagccaaa gggaaaaaac
    17821 tgcacgataa acgtgaagat attaaagacc gtgaatggca agtggctaaa cagcgtatta
    17881 tgaaaaacgc caatcgtggc taaatgaata attaccctct ccccttgtgg gagagggaca
    17941 gaaaaattgc gttcagcaat ttttcaggga aagggggatt tgtaaaagtt caaggaaaaa
    18001 caacgcttgc cctctctctg aaaaatattt ctgaacgaaa tatttttcgg tctctctccc
    18061 ataaagggag agagtgaaac acaaatttaa actaaggaac atacaatgat cgaccaaaac
    18121 ctacttcgta ccaatttaga agacgttgct acagctctta aaatcaaacg taattttatt
    18181 ttagatgtag agcgagtaaa aacccttgaa gaacagcgta aaacgctaca agttaaaacc
    18241 gaaaccttac aagcagaacg caacactcgc tccaaaaaca tcggtgcggc aaaagctcgt
    18301 ggcgaagata tttcagcttt attagccgaa gtagattcaa tgggcaacga attagacacg
    18361 gcaaaagtag aattggataa agtacaggca gaaatccgtg agttgttgct ttctgtgcca
    18421 aacttaccgg cagatgaagt accactcggt aaagacgata gcgaaaactt agaagtatta
    18481 cgttggggcg aaccacgtca attcgacttt gaagttcaag atcacgttac ccttggcgaa
    18541 aacttaaacg gtttagattt tgctgccggt gtaaaattaa ccgccagccg ttttgtggta
    18601 atgaaaggta aacttgcccg cttacaccgt gcattatctc aatttatgtt ggatttacac
    18661 actgaacaac acggctatgt ggaaaccaat gtgccatttt tagtcaatca cgacactcta
    18721 tacggcacag gtcagctacc aaaatttggc gaagatttat tccatactca accattaaca
    18781 gggcaagatc caaacgaggt gcaacgccca ttcggtttga ttccaaccgc tgaagtgcca
    18841 gtaacgaact tagtgcgaga tgaaatcatt gatgaagaca gcttaccact gcgttatacc
    18901 gcccacaccc cttgtttccg tgcagaagcc ggttcttatg ggcgtgatac tcgtggttta
    18961 attcgtatgc accaatttga aaaagtggaa atggtgcaaa ttgtcgcccc tgaaaaatca
    19021 atggaagcct tagaagaatt aaccggtcac gcagaaaaag tcttacagct tttaggcttg
    19081 ccataccgca aagtgctact ctgcactggt gatatgggct ttggtgcagc aaaaacctac
    19141 gacttagaag tttggctacc ggcacaaaac acttaccgtg agatttcttc ttgctcaaat
    19201 atgtgggatt tccaagcccg ccgaatgtct gcacgttgta aagctaaagg cgacaagaaa
    19261 actcgcttgg ttcacacctt aaacggctca ggtttagcgg ttggtcgcac actggtcgct
    19321 gtgttagaaa actaccaaaa tgccgatggc tctattactg tgcctgaggt gttaagacct
    19381 tatatgggtg gtttggaagt aattaccgct taagattaat aaatgaaacc aatctcagaa
    19441 atggggttgg tttttttaat aaatcttatc cctaatcttt ttcaggataa ttcataaaaa
    19501 tttcagtaac tactcggcgg ttttgcactg tatctaaacg aataggattt ttcattctcc
    19561 ctaaacgaaa tgcaataatg gtgacttttt tcggttcatc tattttatct aattgataga
    19621 ataccattat ttcccgttga taaaccgctc gctttctgaa acgtaaatcc ttgcgaaatg
    19681 cgacacctgc ttttggataa aactgtaaac cattgatgat attcgtttga gtatgctccg
    19741 aaaataatcc atagaaaata aactctaata gattattttt aaaatcaaca ctaaccatct
    19801 aactgctccc aagcagttaa atctcctcgt gataaacgtt cagcaatatc agcataaaat
    19861 tgtttcggta cttccctttt tacttggtat tgagctgatt gttcttctaa taaatgttct
    19921 tctatcatct tgatttcttc tgttgtccac tcttcatctt ctttatgtac aacaaaatcg
    19981 gtatctacaa tcacaataga catacctagc tccttcaagt actggttata tattctgtat
    20041 tttgcctgtt ctaaattcag ctgtctagct tttctgttac tcacaatttg caaatttttc
    20101 atcaaattcc accgcttgcc attcaaaaaa aggcgttttt gcggtatcct atcggcaatt
    20161 ttttcagtta aatttaaagg ttaaatttta tgcaacagca gtataaccca agtgagattg
    20221 aacctaaggt tcagcgttat tgggcagaaa ataaagtttt taaagcagtt aaagacgtta
    20281 ccaaagagaa atactattgc ctttcaatgt tgccttatcc gtcaggtaag ctacatatgg
    20341 ggcacgttcg taactacacc attggtgatg tagtgtctcg ttatcagcgt atgatcggta
    20401 aaaatgtatt acagccgatg ggttgggacg ctttcggttt accggcggaa ggtgctgcgg
    20461 tgaaaaataa taccgcaccg gcaaaatgga cttacgaaaa catcgaatat atgaaaggtc
    20521 agcttaaaat gttgggcttc tcttatgact gggatcgtga agtcaccact tgccgtccgg
    20581 aatactacaa atgggagcaa tggttcttca cagaacttta taaaaaaggc ttagtgtata
    20641 aaaaaacctc aaccgtaaac tggtgtccga atgatgaaac tgtgcttgcc aacgagcaag
    20701 ttcacgaagg ttgttgctgg cgttgtgaca ccccagtgga acagcgtgaa attccacaat
    20761 ggtttatcaa aatcactgac tatgctgaag aattactcaa tcagttagat aatctcccac
    20821 agtggccgga tcaagtaaaa accatgcaac gcaactggat cggtcgttcc gaaggggtag
    20881 aaatcacctt taaaattgcc ggttcaaacg tagaattacc ggtttacact acccgtccgg
    20941 ataccttctt cggcgtaagc tatgtggcga ttgctgcggc tcacccatta gcagaattag
    21001 ctgcacaaaa taacccggca ttggctgaat ttatccgtga agcgaaaaac accaaagtgg
    21061 cagaagcaga attagccacc atggagaaaa aaggaatggc aaccggcttg ttcgccattc
    21121 acccgttaac aggaaaagaa gtaccggttt gggttgctaa ctttgtgtta atgcactacg
    21181 gtacaggtgc ggtaatggcg gttccggctc acgatgagcg tgactttgaa tttgcacaaa
    21241 aatacgattt gcaaattaat caggtaattc aaccgcttga tggctcagag tgggatttca
    21301 gcaaagccgc ttacaccgaa cacggtaaat taatcaactc tgccgagttt gacggcttgg
    21361 aatttgatgt ggctttcact gcgattgccg ataaattaga ggcaatgggt gtaggtaaac
    21421 gccaagtgaa tttccgtctg cgtgactggg gtgtttctcg tcaacgttat tggggtgcac
    21481 caatcccaat gatgaccaca gaagagggcg aagtggttac cgttccatta caagatttac
    21541 cggtgattct ccctgaagat gtggtaatga acggcgtgca aagcccaatc aaagccgacc
    21601 cagagtgggc gaaaaccact tacaacggca aaccggcatt gcgtgaaacc gataccttcg
    21661 atacctttat ggagtcctct tggtattatg cacgttatac ttgcccacaa tatcacgaag
    21721 gaatgttaga ttcagacgag gcaaactact ggctaccggt cgatcaatat atcggcggta
    21781 ttgaacacgc cacaatgcac ttgctctact tccgtttctt ccataaattg ttgcgtgatg
    21841 cgggtatttt aaattcggac gaaccggcga ctaaactgtt atgccaaggt atggtgcttg
    21901 ccgatgcgtt ctactacacc agcccaacta acgagcgtat ttgggtcagc ccgacacaag
    21961 tcaccttaga acgtgatgag aaaggtcgta tcatcaaagc aaccgatcca caagggcgtg
    22021 agctggttca cagcggtatg actaaaatgt cgaaatcaaa gaacaacggt atcgatccgc
    22081 aagaaatggt ggaaaaatat ggtgctgaca cagttcgctt gtttatgatg tttgcctccc
    22141 ctgcggaaat gacattagaa tggcaagaat ccggcgttga aggggcaaaa cgtttcttag
    22201 gtcgtgtttg gaatttagtt tatagctatg cccaaaatcc ggcaacgact gcattagatg
    22261 tgaatgcctt agacaaggat caaaaagaac ttcgccgtga tgtacacaaa acgattgcta
    22321 aggtgagtga tgatattggt cgtcgtcaaa ccttcaatac agcgattgct gcggtgatgg
    22381 aattgatgaa caagctcacc aaagccccac ttgaaaccga acaagataaa gccgtaatgg
    22441 cagaagcttt aagtgcggtg gtgcgtatgc tttacccaat cacgccacat atctgctttg
    22501 agttatggca agtgttaggt aatgaacaaa ccatcgattt tgccccttgg gtacaagccg
    22561 atgaatccgc aatggtggaa gatgaaaaat tagtggtagt acaagtcaac ggaaaagtgc
    22621 gtggcaaaat cactgtttca gctactgcta cggaagatga agttaaagct atcgctaaag
    22681 ccgatccaaa cgttgctaaa ttcttagatg gtcagcagat tgtaaaagaa atctacgttc
    22741 catttaaaat gttgagcttt gcggttaagg cgtaacaaac acactctccc ttatatggga
    22801 gagagaccga taaatatttc gtgcagaaat atttatcaga gagagggtac aagcggttat
    22861 tttcttggaa aattttgcaa aaaatagccc cctctccctg ctttttcttc taaacgaaga
    22921 aaaacctgtc cctctcccac aaggggagag gggaataaac aaggaaatct cattatgatt
    22981 aaaaaactct ctctcatcgc tgtttttgct cttaccgcct gcggttggca ctttaaaaac
    23041 aatgaggtat tacctgaatc gttcagaaca ttaacctttg aaagtgcaga tcaacacagc
    23101 gaaatgtctc gaattttacg caatcagtta caactaagcg atgtaaaatt agtgcctagc
    23161 acggctaatg tggcaaaatt acgtttggtt tcaacctcaa cagacagcaa agtggtttct
    23221 gtgtttaaac aagctcgtga agcagaaaaa atcttaacgc taaacgttga tgcgattgcc
    23281 gatattccaa ataaagggca atacccttta gaagtttcgg ttcatcgtac tttctttgat
    23341 gactctcgtg cagctttggc aaagtctgct gaaaaagaga tgattatgaa agatatgtat
    23401 gaacaagcct ctcgccaatt aattatcaaa atggcgggct taaaaaaaga actctcaaaa
    23461 taatcattgt aggggcgaaa tatgtttcgc ccattgttta tctgtatgca aaaaatcttt
    23521 ccggaaacac ttcaattttc attaaacaaa ggactacagc ccttttatct attaacaggg
    23581 aatgatctgc tattggtgaa tgaaaccaaa gatgccatta ttcacaccgc ccgtctcaac
    23641 ggttttgatg aaaaaaaaga agttgaaatc aaaaacgata ctaactggga agaacttttc
    23701 gaagcagctc aatcaatggg attgtttttt gcacgccaaa ttatggtgct gaattttccg
    23761 gattccccca cagcgacaca gtttaaaaaa ctggctgagc tttgtgcctt tattcatagt
    23821 gatttgctct ttatcctgca cttacctaaa ttttccaaaa caatggaaaa acaggcttgg
    23881 ttcacgcaaa ttgagctaaa tctcgtgcaa attaactgcc aaacaccgga acttagcaaa
    23941 cttccacaat gggtcagtca tcgagcaaaa gcgatgaatt tgcaaattga aaatgaagca
    24001 aaccaactcc tttgttatag ctacgaaggc aatctactgg cgcttaaaca ggcattacaa
    24061 ctgctacaac tcaatgcaac tgatggaaaa atcacgctga caaaagccca agaagtggtt
    24121 gaacactccg cccaatttac tccatttcaa tggattgatg cattatttga aggaaaaatc
    24181 caaagagctc aacgcatttt gtaccatctg caaaatgaag aagtacaagc ggtcgtttta
    24241 ttaagaattg tgcaaaaaga gttgatgatt ttactcgaaa tcactcgcaa cccaaccgca
    24301 gtcgtttcca gcgaacaatc actttttatc ggtaatttac gaacagaatt tgaccgcttg
    24361 aaaatttggc aaaaccgcag acctttttac aaccagatca ctcaacgact gacttaccaa
    24421 aaactttacg ccctaatcca acaactggct gaattagagc gtaaggtgaa acaagaattt
    24481 agcgatgata tttggcaaga attagagcgt ttttccgctc aatttaccta actaatactt
    24541 ttctaaatct tcgctaaaca actccgcttc ttcactggcg atagagtgct tttctgccgc
    24601 ccaatcgcct aaatcaataa tttgacaacg ctcgctacaa aacgggcggt atttactttc
    24661 tttactccac accacttctt tttcacaagt cgggcagttt acaatagttt ctggcattct
    24721 ttttcctttt ttgctaatgc taagtattta taatgtaagg cttgaacctg ctctttaatt
    24781 aaaagcaagc cctgttcaaa tggcaagtta ttttcaatca catcatcagc ataacttaat
    24841 ctttcttcac ggctgacttg ggctgccata atatttttaa tggtttcaat tttactgtta
    24901 tcacgtttgg ttgctctttc cagttggatt tcaggagaaa catcaacaac caatatacga
    24961 tcacaatatt cggtgagctt attttcaatt aaaagcggca cgacccaaat cacataacaa
    25021 gcggtcgatt cttgcaattt ttttaccatt tcttctcgaa tggcggggtg aagtaaatta
    25081 ttgagccacg ttttttcctc ttcattgtta aacacaattt ggcgaagttt agtgcgttct
    25141 aatgcttttt cagcggttaa gatctcttca ccaaaatgct ctacaatctt ggctaaaagc
    25201 ggtgtacccg gttcaacaac ttggcgagcc accacatcag catcaattac ctctacccca
    25261 agagctgaaa ataaatcaga cactgtggtt ttgccactgc ctattccacc ggttaagcct
    25321 acaacgtaag tcataaaaat tgattcctca aaataaaaac aaaccaattc tagtaattaa
    25381 tccctctgct ttcaagcatt agcaccagca acatcgctgt gctaataaac ggaataaacg
    25441 ccaattttgc taatttctgc tttttcatta gccaataagc caagtaataa ccaatcccta
    25501 gcaggctggc ggttaatatc aataagagca ttttttcgag ccgaaagagt ggggataacg
    25561 caaccaataa caacatatcg cccgagccaa atgcctcctt tttaaacata agctgtgaaa
    25621 ccaagccaat caacaggcaa aaagcaaggg taaaggtaaa agtttggtgt tggaaatcgg
    25681 ctaaactctc ccataacacc aacaaaaata taagcacgag gtagcgaata tccgttaggt
    25741 aatagcacat atccagcagt gataaaaaac agagcagaat aaaaatcgtg ccgataatag
    25801 ggctttccga gcagagcata atcaatggaa aaaggagaaa aaatagattt gccgatcggg
    25861 gttgtttcgg ttgtagcacc gattgagcca aaaactgttg ctcggttaag ccaatattca
    25921 ctaatgaagt tgtttcttgg taaatttggc gattgactcg aatggcaaat cgagggatct
    25981 ccgctttgag ccaaaccgca atgaagaggg aaaacagtat taacatcgct actccaccaa
    26041 agcccccata tcaaaaatcg gtaaatacag cccaacaata atagtgccga caatcacgcc
    26101 cattatcaac attagcatcg gttctaatag ctgagacaaa atatcaattt ggtaatctaa
    26161 tttttgttgg tacatctcac taatatgctc acacattttc gccacattgc cacttttctc
    26221 cccaacctca agcatttgtg ccatctcagc accaaacaca ttcgggttga tcccttgtga
    26281 aaatttgtag ccttgttgta acaaattaag catagtgcca atttcttgct gtaaaatcgg
    26341 gtcatcattt ttctcggata aaaacgacct caaggcaaca tcaagcggaa tatgagcatt
    26401 cagcattaag gcaatatttt ggctgaaaaa gataatgcga gattgctcaa taattcggct
    26461 aaacagtggc attttagccc atagagccat tttccacttt gcgactaacc tgctcttttt
    26521 tgcaaaaaac aggtaaaaaa tgaccgctag tagtaacgtg ccaagtaata ctgtgccttg
    26581 ctcaatcaag cattcggaaa gggtaaataa aatatcggta atgaggggta aagttttgtc
    26641 ttttgagcca taaagttggg caaactgcgg tacaatcaaa accaatagcc cgatagataa
    26701 agcgagcgaa atcagcaaca caatcaccgg gtaaaacagg attttcttca cttttttcgc
    26761 caatttttcc aatttcgctc ggctttcagc taaattagtc agcataatgc ccagttttcc
    26821 gctttgttcg cccattttga ttagctgaat ttcctgattg gtcacaaatt cacccaattt
    26881 ctccaacgat tgtgaaaaag catagccact ttcaatatgc tgaatcagct cattaagcca
    26941 tagataaagg cggatatttc ggcagttttg caacaacata gataatgcct gttttaacgg
    27001 catggcagaa ttaaccaaca atgcaagctg tgatagcaag ctattcacct cttctttttt
    27061 cgggtttgct gaaagcacaa aatttcggct aatccgaata tgctgataac cttgtgccag
    27121 caaaaggttt tccaacatgt ctcgattcgt tgccaaccgc ttgccccgct gtttttgccc
    27181 aaatcggctc tgggcttgcc aatgaaattc atagattttc gtcattgcct aagcaatttc
    27241 tctttttgtg gttcaaaatg aatatctgag gcgttgtgtt gcaaacagaa cttaaataaa
    27301 ttctctaaaa tttggataat cggatcgttt ctatccactt gttccgaatg agccaatgaa
    27361 tacgccgtat tctcctcata aatctcactc tgccgactac tctgctcagg cgaaagggca
    27421 ttgagtaaaa acttcaattc atcggaggaa atcaaaacag gctcaatctg tttgtggtag
    27481 ataaacgcaa aaatctcaca ggcattgaga ttttgcatat catcaatccc tagccaaagc
    27541 tgttcttctg tttcttgcag cggaatggct aaatatcgca ataaaatctg cttttcattg
    27601 caatttttct gccactgctc tgctgaaatc tcgatgaacc tttggctgtt taaatccgtt
    27661 accgaatact ccattttgat ttcctcacct caaattagcc gtttggtgta acagttgtgc
    27721 tacagaaatt agctggaaat aggctcgtat cattgccact acaacttgcc gtccaactaa
    27781 tcgtattatt tgagaacgac ggtgttaagg tatagccata accattcaga gagcctttgc
    27841 cggttaccgt aatcacgcct tttgttaccg caattgagtt taggtattta gtggtattac
    27901 tactggtttt attcgcctga atcccattcg tgccaccgga gcagttatcc aacccgccac
    27961 tgttgtaaat acagacttca acgtctgatt tatacgaagc agatgcttgt aataactccg
    28021 ataacgccgc cttttgggtg tatgagttat aggaagggat cgcaatagtg gcaagaatgg
    28081 caataatcgc aatcacgatc atcaactcaa tgagggtaaa tgcccgatgc agttggcgaa
    28141 tgccaagcgg tctaaaatgt acaatttttt gcatataaat tccttataaa ttgcaccaac
    28201 aaattgtggc gaatcaagtg tgcaaatttt ttgcctgctt tgcgaccggc aaattttaat
    28261 ttttcgacgc agatcgaaaa aataaaattt ttatctgttt atccatacag ttttttgtta
    28321 gaatttgcca aatttactca attaggaatc aagaatggca aaagcaccta aaaccgctta
    28381 tgtttgtaac gattgcgggg ctgaatattc ccgttggatg gggcaatgta aggaatgtaa
    28441 ggcttggaat accatcagtg aagtgcggtt gatttccagc aaagaagcgg cgaaatctga
    28501 ccgctttagc ggctatgccg gtgaaacgtc aggcaaagta caaacgctgt ctgacatcag
    28561 cttgcaggaa gtgcctcgct ttagcagtgg ctttaacgag ctggatcgtg tactcggtgg
    28621 cggcgtagtg ccggggtcag caattctgat cggcggtcac ccgggggcgg ggaaaagtac
    28681 gttattactg caagtaatgt gcggtttagc ccaacatctg ccaacgctgt atgtcaccgg
    28741 tgaagagtcg ttacaacaag ttgcaatgcg ggcaaatcac ttaggcttac caacggcaaa
    28801 tctgaaaatg ttgtctgaaa cctctgtcga gcatatttgc aatattgcag atcaagaaaa
    28861 gcccaaaatt atggtgattg actcgattca ggtgatgcac ttgtcggata ttcagtcctc
    28921 acccggcagt gtttcacaag tgcgtgaatg tgcatctttc cttacccgct acgccaaaac
    28981 ccgccaagtg gcgattatta tggttgggca cgtcactaaa gatggcacgc ttgccggccc
    29041 gaaagtgttg gaacacgcca tcgatgcttc cttgctgtta gaaggcgaat ccgattcccg
    29101 tttccgtact ttgcgtagcc agaaaaatcg ttttggtgcc gtcaatgaat tgggcgtatt
    29161 tgcgatgacc gaacaagggc taaaagaggt taaaaaccca tctgcgattt ttttaagccg
    29221 tagcgaagag caaacgccgg gcagttcggt gatggtgtta tgggaaggaa cacgcccatt
    29281 gttggttgaa attcaagcct tagtcgatca ctcaatgctg gcaaaccctc gccgtgtggc
    29341 ggttggctta gatcataacc gtttatcgct attacttgcg gtgctacacc gacacggtgg
    29401 cttgcagatg tcggatcaag atgtgtttgt aaatgtggtc ggtggggtga aagtcacgga
    29461 aaccagtgcc gatttagcct taattttggc gttaatttcc agcttccgta accgtccatt
    29521 gccgcaagat ttggtgattt ttggggaagt cggcttagca ggggaaatcc gcccggttac
    29581 cagtgggcaa gagcggatca gcgaagccgc aaaacacggc tttaaacgag cgataattcc
    29641 tcacggcaat gtcccgaaaa aggcaattaa agatatgcag gtgttcaccg tgaaaaaact
    29701 cagtgatgcg ttagatattc tgtataatct ctaaaatcaa gaaccgcttg taagtctgtc
    29761 ttacaagcgg tctgttttag gtaaaaattt acgaatgtgc ggcagttggt ctattgcctt
    29821 tggaaatata gtataaatta atacacacca ccaacgcatt taagccggtg accggaatcg
    29881 agccaatcag caaaccataa acaacgaaac aggcacaacc cagcgaattc accacccgca
    29941 gtttaatcac atcttttaat aaaaaagaac aggcaactaa aatcatcgcc acatagccga
    30001 gtatttcaac atagtccatt tagaacctcc tcaaataaaa taaactgccg atattttaaa
    30061 ggctgttttt ttatttgtat ctagctctaa aaatagcaaa ggtttgcgtt cacattttta
    30121 cttttaaccg cttagttatt aaacagattt tcgttctaaa cacataaaac ggcaacggaa
    30181 agggttattt tcatcaattt ctgaccactt ttcttctact aacgcccaat tttgttcatc
    30241 aaattcaaag aacgtatccc cttcaatgtc tgcttgaatc tcagttaaat aaagtctttc
    30301 tgctagtggt aaggcttgct taaacagttc ccccccacca ataatcatta tttcagctgt
    30361 atttgcaaaa tttttcgcta attcaaccgc ttgttcgagc gattgtaccg cataagcccc
    30421 ttccaccgta aaacctgaac gggaaagaat aatattaggg cgttttggca ataacctgcc
    30481 gatagattca taggttttcc gccccataat caccggtttg ccgagcgtgt tttggcgaaa
    30541 ccaggctaaa tctaccggca aatgccacgg catttggtta tctttgccaa tcaccttgtt
    30601 gattgttcgg gcaacaataa tgctgatttt cattttttct cctgttatgc aaattggttt
    30661 gattttatat caaattaagc gtgattttac accctaaaag tgatactttt ggcaacatta
    30721 tttttagtta caagtgaaca gattatgtca aaaactatcg tgataaaatt tggcaccagc
    30781 actctcacac acggcactaa aagtttaagc cgtccttata tgttagagct ggtaaagctc
    30841 aattacacca acagcatcgt gtgattgtgg tgacatccgg agctgccgct gccggtcggg
    30901 actatttagg acaccctgaa ctccctaaaa ccttagcctc taagcaaatg ttagcagcgg
    30961 tcgggcaaag tcagctcatt cgagtgtggg aaaatttatt cgatatttat aatatccata
    31021 tcgggcagat gctcttaacc cgagccgatc tggacgatcg tgaacgtttc ctcaatgccc
    31081 gtgataccct tgatgccctg cttgcacaaa aaatcattcc ggtgattaat gaaaacgatg
    31141 ccgttgctac cgaagaattt aaagtaggcg ataacgataa tttatcggct ttagttgcga
    31201 ttttggctca agcagatcag ctctatttat taaccgacca agaaggctta ttcagtgccg
    31261 atcctcgctc aaaccctgat gctaaattgc tctcggtggt ggaaaaaatc acgcctgaaa
    31321 tccgccaaat tgccggtggt agttcaaccg gtttaggcac gggcggaatg agtaccaaaa
    31381 ttactgctgc tgatattgct acccgctccg gtgtggaaac catcatcgcc tccggttctc
    31441 gcccaaatgt gttagttgat ttagcaaacg ggcaaaacat cggcagtaaa tttttagtcc
    31501 aatctgaccg cttggaaggc agaaaacgct ggttatttgc tgctccgcaa gtgggtgaaa
    31561 ttgtggtaga tttcggggcg gaacaagcct tgcttcaaca taaatcatta ctgccggtgg
    31621 gagtttctgc catcaaaggc gaatttactc gaggtgaagt aatcaaaatt ttcagccaag
    31681 acggcaaagc cttagcactc ggcattgccc gttatagcag tgaggcattg ctgcgaatta
    31741 aaggtaagaa atcaaaagaa attgaatcgc tccttggtta tgaatttggt tctgttgcag
    31801 tgcatagtga cgaaatggtg gtgtattaat ggaattggga attgatgttt tagcaatctt
    31861 gtttgccgtt gcgacccttg ccggctttat cgatgccatc gctggcggcg gtggcttact
    31921 cactattcct gcattaatgg cagcagggct tcctccggca atggcacttg gcacgaataa
    31981 attacaggcg tgtggcggat cactctcttc ctcactctat tttattcgca aaaaagctgt
    32041 taatttaaaa caaatttggt tgctaattct atgcactttt ttaggctctg ccgccggtac
    32101 tatttttgtg caaatgatca atgtcgattt cttaaaaaca ttgttgcctt tcttaattct
    32161 gattatcggc atctattttt tggtcagccc taaaattggc gatgaagatc gtaaacaacg
    32221 cattagcttt accctatttg cctttaccgc cgctgtcgct ctcggttttt atgatggaat
    32281 gttcggaccg gcaaccggct cttttatgac tctggcattt attttgctgc tcggcttcaa
    32341 cctctctaaa gccgtagcac acgccaaagt gattaatttt acctccaact ttgcctcatt
    32401 aattttcttt acgcttggtg gtgcggtaat gtggaaaatc ggcttaatta tgatggtcgg
    32461 gcaatttatt ggcggcacac taggtgcgaa aatggtgatg acaaaaggca aaaaattaat
    32521 ccgtccgatg ctagtgacta tgtcttttat gatggtcggc aaaatgctct acgagcaagg
    32581 aatttttaat taacaatgaa taaagcaaaa cgtattgaga ttttaacccg tttacgcaat
    32641 gaaaatccaa aaccgacaac tgaactcaat tacagtaatc cgtttgaatt attaattgcc
    32701 gtgattctat ccgcccaagc gaccgataaa ggcgtaaata aagccaccga taagctcttc
    32761 cctgtcgcca acacgccaca agcgatttta gatttaggcg tagatgggct aaaagagtac
    32821 atcaaaacta tcggcttatt taacagcaag gcagaaaata ttatcaagac ctgccgagat
    32881 ctgatcgaaa aacataacgg cgaagtgcct gaagacagag atgccctcga agcccttgcc
    32941 ggtgttggca gaaaaaccgc caatgtggtg atgaataccg cattcggaca ccctacgatt
    33001 gcagtcgata cccatatttt cagggtgtcg aaccgcacga acttcgctcc cggcaaaaat
    33061 gtagttcagg tagaagaaaa actgctcaaa gttgtgccgg acgaatttaa agtcgatgtt
    33121 caccattggt tgattttaca cggacgctac acttgcattg cccgtaagcc tcgttgtggt
    33181 tcttgtatta ttgaagattt atgtgagttt aaagagaaga cggaataaca ttatctggtg
    33241 gtatttaatg aataactccc ctctttagaa aagtaggggc tgggggagat ttgatactat
    33301 ttcaacattt cgtatgagcg tatagaaatt ctgccaaatc ttccctatcc cctctttgct
    33361 aaagagggga actagatcga gcgtaaaata taacaagcgg tcattttttt gagatttttt
    33421 gcaaatggat atttatttag tcggtggagc agtgcgggat caacttcttg ggctgccagt
    33481 gaaagataga gattttttag tggttggcgc aactgccgaa caacttttag cccaaggatt
    33541 tcaacaagtt ggggcagatt ttccggtgtt tttacaccct gaaaccaaag aagaatatgc
    33601 ccttgctcgc caagaacgca agaacggcaa tggttacaac ggctttattt gcgatttctc
    33661 cccaaacgtc acgcttgagc aagatttgat ccgccgagat ttaaccatta acgccatcgc
    33721 ccaagatgaa aacagcacac tttttgatcc ttatggcggt gtgcaagatt tagaaaatcg
    33781 gctactccgc cacgtttctc ctgcattcag cgaagatccg ctacgggtgc tacgggttgc
    33841 ccgctttgcc gcccgttttc attcatttgg ttttaccatt gctcctgaaa ccctgaaatt
    33901 aatgcgagag atggtacaaa gcggtgaatt gaaacattta accgctgaac gtgtgtggtt
    33961 ggaaacacag aaagcctttg aaaccgataa tccgcacgtt tattttgaca ttttacgctt
    34021 aattggggca ttaaaagtgc tattccctga actggatcgt ctgtttggcg taccacaacc
    34081 acctcaacac caccctgaaa ttgactccgg caaacacact ctaatggtgc tacaacaggc
    34141 aaaaagactt gcaaaaaaag cggaaaatcc gaccgcttta ctctttgcag ccctttgcca
    34201 cgacttaggc aaaggcttaa cgcctgctga tattttgcct catcactacg gacacgaagt
    34261 caaaggcatt caaccaaccc gagagttagc caatcggtta aaaatcccaa ctgagattaa
    34321 agattttgcc cttttagtga ccgaatacca cacgcattgc cataaaatgg cggaacttcg
    34381 ccctgaaacg gtaataaaac tgtttaacag cctagatgtg tggcgtaaac caaaccgctt
    34441 ttttgatttc ctgttcgcct gtgaagcgga tgctagaggg cgattaggtt ttgaacagcg
    34501 gaattacccc caacctgaat tggcgaaaaa atattaccaa gccgcaatcg aaattgatgt
    34561 gcagcaagtt attcaagacg gttttgaaaa acaagcaatt cgggaagagc tgaataaacg
    34621 cagagtgtat gcggtaagaa gcatcaagct agatagcgac ctaataaacc attaggttat
    34681 ccgccacttt ctcaaaatca attcccaaag ttcaagttgg ttaaatgtat ggattcttta
    34741 aaaggtggcg ttattttagt gaatttataa tgttcctatg ctcttgattg ttgtagccac
    34801 aatttcaggg taaagatcag caataaccca atgccgaaat tggcaatagt gaagccggct
    34861 aacccaataa aagataaagg ttcaaattga taaaattcaa tcgcacctct tgccattgca
    34921 gctaaaccaa atgaaaatgc ccagaaaccc atcgagaagc cattttcagc tatccatttg
    34981 aggtttcgta aaataaaaca gaattgtaag aatccatatc cccataacat ttttgccaac
    35041 aagtctaatt ctccacccat agaaaaatag gcagaagcgc ccacaaatgc cggagcaaga
    35101 acaattccta aagatggtct agcaatcgga aaaagcggac taagccgaat tctttgtaac
    35161 atagtgggtt catatactaa ccaggaaatt atgccaatgc ctaaaaataa agatgcaatt
    35221 tcactgtagc caattaagct taatgaactt gccatagtga agctccctgc aattggcggt
    35281 aggtaatgta ccgggtgtag cgtttccggc aaaaattgtg tgcctttcca taatccacca
    35341 ataataaaaa ccaagaaaat taaatgcaca atgacgccta aaaaaatcaa tagttcgcct
    35401 aaataaaaat cccatacata gagcacatca ccgcttatca tggttgtcat tgtcactaag
    35461 gaaaataaac aacattgcac tgggtgcata aattctgctt taatctgctc aaaacaaaaa
    35521 aacacacgat acacataaca gcctaataag gctacccaaa gtatgactga cgttccagca
    35581 atgatattac caattgttgt agcataagga gtgataggcg ttgcatttaa ccaggcaagg
    35641 cctaacgccc ccagccctaa tggcattgca aaaaagcctg tagttattgg aaaaggttta
    35701 gattcactca taaatttatc ctcatattga aataatgcat aactgtattc ggctttataa
    35761 cagtcatatg taaaaaattt tatttttcta accgcttgta ataagaaaaa cctaaagcac
    35821 ttgctttagg ttcctactac tattcatact cttctagcca gatagttaaa atcgcttcaa
    35881 gaatatgttc atttgatgct tccggatcat catcgaaatc ttccatttca caaatccatt
    35941 tatgcatatc tgtaaaacga accgtggtcg ggtctaaatc cgggttcata tcatacagat
    36001 tttcagcgat cattcgggta tctgtccatt tcattagtgc gctgcctcat ttgcgtgatt
    36061 taaattatat tttggaattt caacaaccaa atcttcttca ccaacaatac attggcaact
    36121 taaacggctg tctatttcca atccccaagc tttatccagc atatcttctt cttgatctga
    36181 agtatcattt aacgaatcaa aaccctcacg aattaccacg tggcaagtgg tacaagcaca
    36241 agaacagtcg caagcgtgat gaatttcaac gcctgcttcg tgtgctactt ctaataaatt
    36301 atctccggct ttagcttcaa caaccatacc ttccgggcaa aattcttcgt gtggaagaaa
    36361 aacaattttt ggcattttaa agtttcctta ttactaatcc cattcaatat tatccagatg
    36421 ttcgccgatg aagtttaaaa tctcttcctc attttcaacc agataaacaa taatcttctt
    36481 taattccctt gctttctctt tacttttctc ggaaagcgga taggtaacat aataacatcc
    36541 tacgtccgga tcttcttcta ataactctaa taggcttgga cgataaatac taagataagc
    36601 ccgtaaaaat gcagcccagt tataaccgtt catataggca acatcatatt cagtattaat
    36661 ccgctcgccc aactcaaaaa tgcggttatc ctctaaatcg aattcaaccg caacggttct
    36721 gaattgttct gaaatctcgg tttttacata gttagacata ctgcctcctt atgctaaatc
    36781 gtccattttt ttaccggcta atgctttttg aattgataaa ttcatacgcc gtgctgcaaa
    36841 ttcttgcgta gctaaatcca cctctttgat accttgctga attgcaaagc gatcagtacc
    36901 ttcttttaat tcgattaaac gggctaattg tgcttcaatc gcttggaact cctccacact
    36961 caacactgca gagccatctt ctttcaatgc cgttaccacg ctgtcaatca ctcggtcggc
    37021 ttctactcgc tgttcaacta actggcgggc ttccatatct tctttggcat ttgccattga
    37081 ggatttaatc atctgcgtaa cttcttcatc ggttaagcca taagacggtt tgatttgaat
    37141 tgaagcctgc actttggtcg atttttccat tgcagtcacg tttaataaac cgtctgcatc
    37201 aacttggtaa gttacccgaa cctgtgcaac ccctgctgcc attgccggaa tgccacgcaa
    37261 agtgatttta ccgagagagc gacaatcttc gactaattca cgctcacctt gtaaaacgtg
    37321 gattgtcatc gccgtttgac cgtctttacc ggtagtaaac tcttgagctc gagctactgg
    37381 gatagtagtg ttgcgtggaa taattttctc caccaaacca cccatcgttt caataccaag
    37441 cgaaagtggc acaacatcaa gcaacaacat atcggaatcc ggtttgtttc caactaaaat
    37501 atccgcctgc actgccgcac caatggcaac aactttatcc gggtcgatag aggttaatgg
    37561 cgttttaccg aaaaactcgc cgacttgttc acgcacaaac ggtacacggg tcgaaccgcc
    37621 taccattacc acttcacgaa cctcttctgc ttcaactccg gcatctttca aggctcggcg
    37681 acaagtcatc agcgagcgtt ttacaagcgg ttgaattagc tcattaaatt gcgaacggct
    37741 tactacgcct gtccaatttg caaatttcac ttcaaattca accgcttgcg aaagtgcgac
    37801 tttggtttga gttgccaaag ttaataattc acgctgctcg cttgccgttt gaggtttgta
    37861 atttgcctgt tcagcaatcc attccgctaa taggtggtcg aaatcatcgc caccgagtgc
    37921 ggtatcacct gcggttgcta atacttcaaa cacacctttg cgtaaacgca aaatagataa
    37981 atcgaaagta ccgccaccta aatcgtaaac ggcaatcaca ccttcttgtc cgctttctaa
    38041 accataagca atcgctgctg ccgttggttc attgagcaaa cgtaatacat ttaaacccgc
    38101 aagacgtgcg gcatctttgg tactttggcg ttgtgcgtca tcaaaataag ccggtacggt
    38161 aattaccacg cctgaaagct caccggctaa acgcttttcg gcaaattgtg ccaagtggct
    38221 taaaatatcc gccgaaactt caatcgggct tttattacct tgcgctgttt ggattaaagg
    38281 taaaccgttt tcgctcgcga caaattgata aggtaaattt tgataacgtt gctgaacatc
    38341 tgccagagaa cgcccaatga gacgtttagc tgaaatgacg gtatttttcg ggtcaagtgc
    38401 agctttggca aaggcttcaa tcccaactgt tttttctgtt tccgcataat gcactactga
    38461 cggcactaat gctctttctt gttcatcaag caatacctga gtctgcccgc tacgcacagt
    38521 tgccactaat gaattggttg tacctagatc aattcctacc gctaatttat gttgatgtgg
    38581 tgcagcggtt tgaccgggtt c
//
LOCUS       DANLXS010000004        37872 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.004, whole genome shotgun sequence.
ACCESSION   DANLXS010000004 DANLXS010000000
VERSION     DANLXS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 37872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37872
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.004"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            52..270
                     /locus_tag="PXD71_000156"
     CDS             52..270
                     /locus_tag="PXD71_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6290325.1"
                     /translation="MARNKRVRDMSNFEIRGELMKIGKSLSQLGIENGLAKTTVRNAL
                     DKPYPKGEQIIAKAIGKTPQEIWPSRYE"
     gene            280..2250
                     /locus_tag="PXD71_000157"
     CDS             280..2250
                     /locus_tag="PXD71_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HDL6290326.1"
                     /translation="MKEWFLAKEIAGLSGVPELPNSVSRLATKEGWQKRQIQGVRGVT
                     YEYHLTSLPIETQQQLRLNAALAVIPQAAELQPKRDDPALIARLNNATDKGRDKAKGK
                     AEACMQLQAFLDQGFSYTQAEAGAATAKNVSQGSLKNWYYKVKGHPVHLWQAILISES
                     GRSKKPQLKAKITEEAWDCFLADYLRPEKPDLRASYRRTQAIAKQYGWQIASLQTFQR
                     RVLAEVPYEVILLKREGANAVAKLVPALQRTVKDILAGEWINGDGYQHNVFVKWHTGE
                     IVRPKTWFWQDVRTRKILGYRTSISENTDSIRHALMDVIFNVGIPKTLTLDNTRAAAN
                     KAMTGGIENRYRFKHTELDPKGIMPILGIDVHFTSILYGEGHGQAKPIERAFGRGGIG
                     EKIDKRPELSGFYTGRNAQETPDNYNGGKDGVDYNTFLKAIAAGIEEYNSQTERHTEM
                     CRGELSFNQVWERDYHPSNVRQASPEQLRLLFLQAETVSIKRNGSFTLKAAGKLYGLT
                     NVYWAESLIGITDKKVVARFDPDDLHGNVYVYNLEGQFLAEAVCREAKGFGDTSASRE
                     QGRLYQKVVKSAKAQAEALELLEAHELASLAPQVDVPEPIEKKVKEVLVKEDFIVDFN
                     TVRKATVVEETEEISIFDEVLLKQEKQLRIVE"
     gene            complement(2430..2918)
                     /locus_tag="PXD71_000158"
     CDS             complement(2430..2918)
                     /locus_tag="PXD71_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290327.1"
                     /translation="MLFRSLTDDEIHQIALDSARATFSGFKLKRANAIVMKNCITLFK
                     LSTINFCLAEYHSSFHHLTSIDDLDGIMPTVKKVAQMLSLQESKALIWLLYLKWLFVM
                     RQRSLQVLLENSADLIQGEVIAQPLPTFVKAVLAFQLETYHGLPEWIPEFDSAEFWQS
                     LS"
     gene            2950..3891
                     /locus_tag="PXD71_000159"
     CDS             2950..3891
                     /locus_tag="PXD71_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6290328.1"
                     /translation="MTLIDQIKQHIAESGSSQGKVAKEAGINAGALSAYLNENYKGNN
                     EELEAKLIAYLERIEAKKREFVEAPSFIETKTANQIFGSLRFAQNTGVLAIIHGASGV
                     GKTQAAREYRKRYANVWLVTASPSRSSLSEVLYEIALELGMNDAPRRKGTLARLIVRK
                     MKGTAGLLIIDEADHLPYEALEELRLLQEEVVNESDIGAGVGLVLIGNDKVYTRMKGG
                     INPAHEYGRLWSRSAKRTSIQKTKKEDTQAVAKAWGLSENQEALKVMQAITETGGGLR
                     ILTHTLRLAAMVAKDRMIPLTADLIQLARKDLLSTGD"
     gene            3894..4208
                     /locus_tag="PXD71_000160"
     CDS             3894..4208
                     /locus_tag="PXD71_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290329.1"
                     /translation="MRTPKPKHSFNRTNQAACRFLKQTQKAIIRLNSLGFTVLNIDFT
                     RIKPRIEVEIGNNKHIAQGLIYEGKAYRYSFGKSEDLGRWEGYYTMLEGIRVWWRQAA
                     IH"
     gene            4220..4456
                     /locus_tag="PXD71_000161"
     CDS             4220..4456
                     /locus_tag="PXD71_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290330.1"
                     /translation="MKTLALLTALLGLVGCDLVQSKQAKNYIGNNLTEICIDNVVYLI
                     YSGDKKGGITPKVNKDFYPYTCTNKEERQNGKTR"
     gene            4440..4733
                     /locus_tag="PXD71_000162"
     CDS             4440..4733
                     /locus_tag="PXD71_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290331.1"
                     /translation="MAKPDETPITKAECQSQLAELGVQYKKLPMAITRHICNATTNIH
                     GKVIKVSVVERVGYGVQITAQGNEKSCLVTYEAMLGMAEAMGLFDEVKEQNND"
     gene            4855..5073
                     /locus_tag="PXD71_000163"
     CDS             4855..5073
                     /locus_tag="PXD71_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL6290332.1"
                     /translation="MNDELAAISASYAQHNRYQHYAEKAAEAERKGNYKEAQDHWEVA
                     KLSAKKTANRDWAEQRAEFCKRMHNKPF"
     gene            5083..5265
                     /locus_tag="PXD71_000164"
     CDS             5083..5265
                     /locus_tag="PXD71_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290333.1"
                     /translation="MTETVKAQLNSQLNEAIIQLIQAQKYLNQDDAIRSGVYIGTVQD
                     LLPKVHLKLLTANRKH"
     gene            5282..5671
                     /locus_tag="PXD71_000165"
     CDS             5282..5671
                     /locus_tag="PXD71_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290334.1"
                     /translation="MAITEEQWKEIKQKLDSIIGRVKFRYKEHLLTVDVVQIKRSLKL
                     AVYVDGEIDGAWTKEGHEIRPYLEEVWYRKERPFFNAKEKKEYRGLMSKKKLNEKIVT
                     YSPMFPSPTALTRQYKKLDGLELIEVI"
     gene            5720..6007
                     /locus_tag="PXD71_000166"
     CDS             5720..6007
                     /locus_tag="PXD71_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290335.1"
                     /translation="MNKNEFFKKYHLEGSLAILDLDANDDQSQFCYQALLEYRELPLD
                     TFLAEYSDDEDMTESEFIELMLDELEWIIRRAKNAKRFVKKRQKTMREEIM"
     gene            6126..6671
                     /locus_tag="PXD71_000167"
     CDS             6126..6671
                     /locus_tag="PXD71_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HDL6290336.1"
                     /translation="MTDKAKLIQLIHIAKSQLAMDDLSYREMLKRLTNKTSSTKCTVV
                     ELHKVLHELQTKGAKVKYFAKRSKKPTAYSPATGETAVKSQIAHKIRAVWITMGKHGF
                     LNDPSEKALNAYMRKVINNKTRPTILLNVSALEAGDASYMLEILKKWHKRVMIKALSK
                     SCTVDPKISYDELVEWYNEVM"
     gene            6658..7191
                     /locus_tag="PXD71_000168"
     CDS             6658..7191
                     /locus_tag="PXD71_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290337.1"
                     /translation="MKLCRCPICHSDIHLEALVEDEAGRELLGKISQLTHGVAQPMVA
                     YLGLFKPQKSNLNNSRALKILNDVLALYPCSLLLAQALSETVVSIRKKRQQAMENGQK
                     IEPLPNHNYLKSVYETQKVHFAVVRTGKTERETMKAELEKTRNAIAYVQRFVDLGREE
                     VVKNSPEYQIWLAHKPK"
     gene            7356..7715
                     /locus_tag="PXD71_000169"
     CDS             7356..7715
                     /locus_tag="PXD71_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6290338.1"
                     /translation="MELVDIFEEKAPEVLTDLAKHIEVALEEKCQFSPEKAKQIGIDI
                     AQQMAQNWGGEVIYIPRNLLMALNERDMKIYEEFNGNNHRELARKYNVSMQWVYKIVK
                     KVHKDEVARRQMSMFEM"
     gene            7851..8360
                     /locus_tag="PXD71_000170"
     CDS             7851..8360
                     /locus_tag="PXD71_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL6290339.1"
                     /translation="MSQNLTFRQIFDRLIGHEGGYVNHPQDPGGETHWGITKRTAVAN
                     GYMYAMKNMTREQAYQIYEKAFWQRYRCAELKPAVAYQFFDAVVNHGFGNASRMLQRA
                     VGVADDGIIGNITLQAINAKSESDLLMLFNAERLKFYTKLRNFPTFGKGWVNRVAGNL
                     EYAAQDNEV"
     gene            8363..8632
                     /locus_tag="PXD71_000171"
     CDS             8363..8632
                     /locus_tag="PXD71_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL6290340.1"
                     /translation="MAKLFKELLSTNGTLSTTSTVQFFGTLCVFGLMVYAVITQQPYA
                     ESLLNNVLIYLFGATTAKGVVTSYQAKIKGGIHKQQSTFKEEEGC"
     gene            8626..8973
                     /locus_tag="PXD71_000172"
     CDS             8626..8973
                     /locus_tag="PXD71_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL6290341.1"
                     /translation="MLMQQILGVIGVIAAILSYALFKSWQLKRERKKNAILQAEKQQQ
                     AVEIEQKKAEVKYVYISKKNNEKVTRSSASDIDDKLQQHGYFRDNNRLHGVQSDLSEP
                     CGYAGNETSDSCS"
     gene            9000..9152
                     /locus_tag="PXD71_000173"
     CDS             9000..9152
                     /locus_tag="PXD71_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290342.1"
                     /translation="MKYIKKLRKRWQIWRFLKNHPEVAQRRDLLAIAIKQGVENPVGR
                     ARRREP"
     gene            9152..9487
                     /locus_tag="PXD71_000174"
     CDS             9152..9487
                     /locus_tag="PXD71_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 domain-containing protein"
                     /protein_id="HDL6290343.1"
                     /translation="MDLFTVFWESIRANFGFISALLTIVVTAFWLKLDSKYAKKNDLG
                     ELLEIAKSHDNRLTTLETKVENLPSAVDVEKLKTLVTDVKGDTKATSKQVDSISHQLG
                     LLLEAKLKE"
     gene            9489..9785
                     /locus_tag="PXD71_000175"
     CDS             9489..9785
                     /locus_tag="PXD71_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290344.1"
                     /translation="MSLRDLLTQDQRLVILRSLAEAGYDANESIISDCLDLYGHDISR
                     DLVRTHLSWLEEQGLLTLERLRDGYMVASITQRGLDVAQGRVVVSGVKRPRPKI"
     gene            9808..10380
                     /locus_tag="PXD71_000176"
     CDS             9808..10380
                     /locus_tag="PXD71_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDL6290345.1"
                     /translation="MSEKNTRGRASKVDLLPPNIKTQLAMMLRDKQYSQSQILEEIND
                     LIRDCGLPDEMLLSKTGLNRYASRMERLGAKIRNAREVAEIWTKQFGEMPQTDIGKTL
                     MEMVKQIAFETSLKLGEQEGGIEPKQLALLSSAIQRLEQAESLSFKREQAIRKEVAQQ
                     AAETAEKVVVQAGLSAETVRTIKEQILGIA"
     gene            10380..11936
                     /locus_tag="PXD71_000177"
     CDS             10380..11936
                     /locus_tag="PXD71_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDL6290346.1"
                     /translation="MALLNNRPLNELAPECRSFLESIHAFNPNELLLGYQKRWIADDS
                     QLKIAEKTRRCGLTWAEAADNALIASTRKSDGGSDVFYIGSNKEMAREYIDAVAMWAK
                     AFNRVAGEIQEEIFEDEDKDILTYVIYFSSGFKVKALSSNPKNLRGMQGVVVIDEAAF
                     HEYLAEVLKAALALTMWGAKVRIISTHNGSENLFNELITDSRAGRKRYSVHTITIEDA
                     CRDGLYQRICQVVTKKTWSPEAEKEWIENLLNDTASEEDALEEYFCVPKFGSGLWLSR
                     ALIERQMSEATPVIRFTAKDGFSLVAEPTRYAEIAEWCELELEPILATLNPDLLHFMG
                     EDFARSGDMTSLVVLAQQQNLVRKVAFIVELGNMPYKQQEQIVMFMLKRLPHFAGGAF
                     DARGNGGYLAEAARDEFSTLIDCVQLSEKWYREHTAPFKAALEDGELEAIPKDADILA
                     DLRSFQVVKGVPRIPDKRTKSADGKSKRHGDTAISLLLAHYASRQLVQMPVEPVSRRP
                     RHSQQLTEGY"
     gene            12055..13509
                     /locus_tag="PXD71_000178"
     CDS             12055..13509
                     /locus_tag="PXD71_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HDL6290347.1"
                     /translation="MAKKNNLISEIATRARSIDYWAFGYYLPNPDPILKRMGKDISVY
                     RELLSDGQVRSGVRRRKAAIKGLEWRITTTNNEKVDEQIYQIFNRLPMNNLITEMLNA
                     ALYGYHISEVIWAKEGELFVPTEIIGKKPEWFMFDDENLLRFRTKENWADGELLPDDK
                     FLLTTQEATQDNPYGLGDLSLCFWAATFKKGGFKYWLEFTEKYGSPWLIGKHPRQAHN
                     TDKERLADALEGMISSAIAVIPEDSSVEIKDAAGKSASTDAFEKFLNFCKAEINIALL
                     GQNQTTEQESNRASAQVGLEVMEDIRSDDQAMIEATFNTLLQWIVKYNFNVEQLPKFE
                     FYEQEAINTDQVERDSKLHAMGVRFSKAYFEREYGFEAGDIEVQAVEKAEKSANTTEF
                     SEGQRRTQVPMPVPILEQLEHEGEAMVDDWLQGVRDKLAQADSLEDFQSQLDSLIPEL
                     SFAEYGELLALGSTVAELAGRQSVNDEIKAKRNA"
     gene            13502..14779
                     /locus_tag="PXD71_000179"
     CDS             13502..14779
                     /locus_tag="PXD71_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL6290348.1"
                     /translation="MRKFTFKQQAQYFEKKLNLRTNSYLDILGEEHDYFFVVAGANRN
                     EVLSEFRKAVDEAIHNGETLENFRKRFDEVVAKTGWEYNGGRNWRTRIIYDTNVYGAY
                     NRGRLQQHLELAEDMPYWEYQHNDNAHPRPQHVAWDGLVLRYDDPFWKYHYPIKAYGC
                     HCTVQALDDFDLKHDGKTVSPPPEIEFEQKTVGVRTGNSRVVTVPKGVDVGFTPHNFD
                     NLKAGRAANVDKLLMQKMVTADPIFASKAVSDLLAKEPVAKLHNQAFKNWVNDVANAK
                     GSALSQVENMQLLGVLPVAVIERLQQINLAPQSAIIAITKGDMVHALRELKQNTGIAV
                     PLEFWEKLPAKLQYPNAILLEKQQAETTLLFIYDVAQTKIAVKVNYDATLQDRHGAKG
                     RQKVLLNRAVTGSLFQDTTGLKSSKYEVLLGEM"
     gene            complement(14981..15325)
                     /locus_tag="PXD71_000180"
     CDS             complement(14981..15325)
                     /locus_tag="PXD71_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290349.1"
                     /translation="MMVNAEQAIELVYDLLLSRQWLVTKAEKLPLDPLSEKEAVMFLY
                     TLDQQTEASWLQLTPEQRATANGLIMDFIAKCLTSTKQWLVSDNIVPELQAIEIIKHE
                     IFLSHNSLVMPN"
     gene            15389..15853
                     /locus_tag="PXD71_000181"
     CDS             15389..15853
                     /locus_tag="PXD71_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL6290350.1"
                     /translation="MIKITLNNTQAVKSLQQIANQLEQPRRLYGILGETLKKIHTARF
                     KAEQDPDGNNWKPLASSTLALKRKRGKSTKILRQDGYLADKTAYNVTSDNVEFGSQEV
                     YARLHQFGGKAGRGKKVTVPARPWLGVGDKDEQLLLRKAEWHLGQIIGRMGK"
     gene            16088..17203
                     /locus_tag="PXD71_000182"
     CDS             16088..17203
                     /locus_tag="PXD71_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL6290351.1"
                     /translation="MTQIEIFKAGKRYDANGTLIDITPEMLQQTVAAYNPEFHEAPLV
                     IGHPKSNNPAWGWVKALSLEGDVLKAEVDQLDAEFSEMVASGKFKKVSAAFYLPDSPN
                     NPHKGVLSLRHVGFLGAMPPAVKGLKQVEFAEDDDFVVFSEWGVASLFSRFRDWLVSE
                     FGLEKADKALPPHEIDWLKEEAMRSQISKQVHAEQATPEPIFNEPAQPEGEPEMSAEE
                     KEELERLKAENEQLKAEKAQAEAEKMEAELEAEKAGNAEFCEGLVKQGKLAPVAKAAF
                     VQALNNLSVLKAGREPEFNEGEDVVSQFKSAMSQSPQIIQFGEYATADKANPQEAEAV
                     EYAEGTDPVSIEADQKVRAYMKAHNVGYAAAFNAIYR"
     gene            17234..18160
                     /locus_tag="PXD71_000183"
     CDS             17234..18160
                     /locus_tag="PXD71_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDL6290352.1"
                     /translation="MTTHNLQKLRVQDPVLTELAQGYHNNELVAETLMPTVEIEKEAG
                     KIPTFGRLAFRLPSTVRNLRGSSNRLDPEDIGAIDVALEEHDVEYAIDYREENEAIFS
                     LRQFALNTTQDVIALGREKAVATLALDESRYETDNKVTLSGTSQFSHKDADVFKVFDT
                     AIRAVKRAIGRKPNVCVISGDVWAALKEHPAVVEKLKYSQVAIVTPEVFAKLIGIETV
                     KIGEAVYEESGQLKDIWSKDIVVAYVAPRSTGGKGTVYEPSYGYTVRRQKGLFVDTYK
                     ESGGKIEVVRTTDIHKPHLVGAAAGYLIKAAV"
     gene            18229..18510
                     /locus_tag="PXD71_000184"
     CDS             18229..18510
                     /locus_tag="PXD71_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290353.1"
                     /translation="MAKFIVLHTPILHDGKRYEVGAEIELTAEQAENNAINLKAVEAV
                     PKPPKNASKPEDEPKPEGEPKPEDAVTEDDVKLAEAKIAEAEQPKGNKK"
     gene            18510..18944
                     /locus_tag="PXD71_000185"
     CDS             18510..18944
                     /locus_tag="PXD71_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL6290354.1"
                     /translation="MYVQIDELLTAFSRKIVVQLSNDEPTATEPDLKMLETAVKVANE
                     RIDAALRSRYTLPLADVPTLINQHALTLARYWLYARRPETKMPDTVKETYQQAVKELE
                     QIATGKLHLGIAESSQILEDDVLADNSEYEVRANARIDTSRY"
     gene            18950..19447
                     /locus_tag="PXD71_000186"
     CDS             18950..19447
                     /locus_tag="PXD71_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDL6290355.1"
                     /translation="MSATQPILEDICQHLRQALPEWEVELFPNDPSTYHLSHINGAVL
                     ISYLASKFEKPRTTQAVLQVRHVQIALTVLTRDLHNDVGALDLLDKLRLLVVGFKPQN
                     CGECWLIDEFFNGSSEETGLWQYQLILQTETQQVQHQAVENLPKFVTAHLRRQDQPGS
                     PGLKT"
     gene            19532..20917
                     /locus_tag="PXD71_000187"
     CDS             19532..20917
                     /locus_tag="PXD71_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDL6290356.1"
                     /translation="MTFHHGSETERVNGGSVPVSVVDSAIIGIVGTAPIGAVNELTVC
                     LTKKDFAKFGTILNQGFTLPDAFDILARYAAGKVYVVNVLDPSRHRTTVTDEVLTLDR
                     NTLTAQTAKTGIIAVTRVKDGANVLNADRYSVDLQTGVVTFVSAPSAPKISYIYADPS
                     KVTEEDIKGGVDSLTGKRKGFELLRDGFNLYGADAKILICPEFDKTASCAAALATLAD
                     QLHAKAYIQLPKGTSLSKAIQGRGPAGTINASASNENVRHFFPYALGSSNTLESLATH
                     AAGLRMLIDVEKGYWFSTSNKELKGVIGLEIPLTARVDDKQSETNLLNAVGITTIFNS
                     FGTGFRLWGNRSSNFPTVTHISNFEVASRTGDIIDESIRQAELQFIDLPIDDALLDSF
                     LETIDTFLRTQKSLVGYSVSLDYEYDLVDAFSRGQVPIVYEYTPKIPGERISNKSVMT
                     RKYLANLVSQR"
     gene            20928..21443
                     /locus_tag="PXD71_000188"
     CDS             20928..21443
                     /locus_tag="PXD71_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL6290357.1"
                     /translation="MSIAINQIVNGNVYINGNSQMGRVNEVKIPDIEFEFIEHKGLGM
                     HGVIKLPAGTNAIEAEITWDSFYPEVRAVMLNPYKYTQLQIRSNLQVFNSQGLAAEEP
                     MVTIMNVSAGKIGGTGHKNKENAEFADTLNVHSIKQTVAGRELLFIDVLANIYRVNGV
                     DVLQKFRTNIG"
     gene            21539..21853
                     /locus_tag="PXD71_000189"
     CDS             21539..21853
                     /locus_tag="PXD71_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL6290358.1"
                     /translation="MANAAQQVLTNLRVQTTYKLQYPIALPDGRVIETLDVRRPKGKD
                     FRLLDEKGFDPEKDGVKILRFYIQQLTTLVPEDIDELDAADIKGLNNLLEDMLIEGKS
                     DK"
     gene            21850..22002
                     /locus_tag="PXD71_000190"
     CDS             21850..22002
                     /locus_tag="PXD71_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL6290359.1"
                     /translation="MSELDTVYADLAWWFKFSPSELLELDLVDIPKWVEQMNRQIKAG
                     YGQILR"
     gene            complement(21981..22283)
                     /locus_tag="PXD71_000191"
     CDS             complement(21981..22283)
                     /locus_tag="PXD71_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290360.1"
                     /translation="MLTKLNNRITEWAGFEACVTRTECTAKWLLAVALVGWSIFYWLD
                     AIQSIQSLSEGVSFVLIYGLILTILAWVGVYLAWGVAILLTLATEPFIWLYRKICP"
     gene            22331..24988
                     /locus_tag="PXD71_000192"
     CDS             22331..24988
                     /locus_tag="PXD71_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6290361.1"
                     /translation="MANNLAIGMVIGASLSSGFHGAFSGAKKKLEQLEQAVAKNQQSH
                     SKLGSELTALRSKQATLYAEMSRASSKGGAGLALMQQEYKKIGKEIANISREQRKWST
                     ELEKSIAKQTKLRNAMERHTQAKENRDELKGKIIGTVASAAMGVGVMKTYMEQEEAAN
                     NLKISMLKADGSFGKFEEIGKIADTLGTDLPGTKKDFYKLAMALKKQGVSDDVLTGGA
                     LKTSAELNVLLDMDQESGGEFLAKFMESHRLNENELPQAADYLQRAMFAGGLSKEQMY
                     ESMKYYAPKLNSMKLTGAENTEKVLAIEAMAGQQGLEGSTFGTGLNMMLSRMNKGPEM
                     LKAAAKGMKAEAREMMEAVGVEFNFWDKNGSFKGIDGMLAEMTKFEKIRKKFGDKGVG
                     LVAEELFGIEGGRLADILAMKGQKGLDEMLSKMREQASLQDRINLKTNTLGAALESLG
                     GAWETFVGDIGSVFAEDIKGFSKGLQSAVEWLVPFVSKHKEAIKFVGGLVAGLVILRG
                     VLQAGSFVIQTVTMAMNALKIAAMTNPIVLVIGAIAAGAYLIYSHWDSIAPWFANLWQ
                     NVTAYFNQFWTWVKGVWSGVTDWVSSVWNGVATFFGQLWTTITSLFSVAWTNIQTIWG
                     GATAWFSGLWDGIKSAAFGMFDSISQKVSSVVASIKSFLGFGDDVDKMGAKIGNVATQ
                     VISKEHSGQLSKTADMAANTGFSSGGFTGQGGKYTPAGIVHKGEYVMTKEATSRLGVG
                     MLDSLNYGGAMGFTQPVLQPILGKQKGFFGSLWDDVKFGANFVGNLLGLNQGSLKTPD
                     FNPNATNASGQNSSIFSNYEPLNRNAVTQQESSQAPQIVVNFNPTINVNGNATQEAQS
                     QIMQQLQMSAYEFEKLLNRVLDQRSRRAY"
     gene            24998..25921
                     /locus_tag="PXD71_000193"
     CDS             24998..25921
                     /locus_tag="PXD71_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6290362.1"
                     /translation="MYFMLGNIAFEPVNLTEFSEQHTAEFAEHAVLKGKPRLQAMGEK
                     LNELNFSLRLHHTIGGVESRYQALLEAKAKQAALALIWGSRYKGDYVIVDISSTTLFT
                     DGRGNALAREMQISLKEFVGDNDGGVLGEALNFGGGSLLGSILPSGAVTTLSQIKTAV
                     SRGVELYNSGKRLVDEVQNTVAIIRQLKNDPATALAYLPAALANLDGALGHFGNLTGS
                     SATLGGLQEKLPAAVVFSEEIGEIYQSLQTMRSSLGNASANSWDNWFTPAESALTEVT
                     DSFDHLAKPVAKMTAWIVLRTDEEAENDTVA"
     gene            25905..26132
                     /locus_tag="PXD71_000194"
     CDS             25905..26132
                     /locus_tag="PXD71_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL6290363.1"
                     /translation="MTQLLKHIVKQGERWDNLSYQYYGNAFEYGRIIDANPHIGFCEV
                     LPIGVTIYIPVLNVKPTNNENMPPWLRGRDA"
     gene            26125..27189
                     /locus_tag="PXD71_000195"
     CDS             26125..27189
                     /locus_tag="PXD71_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290364.1"
                     /translation="MRNVPKPDFSLFYEKTNITAEIEPHLVQLTYIDHLEGQSDELTV
                     EFEDIQGKWIRQWFPTQGDKLKAAIGYQGEFLTDIGEFEIDEVEYSYKPSTINLKALS
                     TGISKANRTLKPKAYENTTLAQVVAKVADNLKLKVVGKIKAIPIKRITQYQERDVEFL
                     ARLAREYHHSFKIVGNQLVFTDKSELGQSEPVLMLEERDTISLRLRDRIKDTAKAVEI
                     SGYDTSGKKVVKKRKKAKALRPNIKQAKSASEDTLKIITRGESQEQIDARGEAALATQ
                     NEDQAAGDIELIGNPKLVAGATILLRNFGVFSGKYLIKSSRHTISRSGGYTTHIEVRM
                     LEFIADDLITLGMEAGNANA"
     gene            27176..27712
                     /locus_tag="PXD71_000196"
     CDS             27176..27712
                     /locus_tag="PXD71_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL6290365.1"
                     /translation="MQTHNFGATYQEGIVSAVDPKTHKVRCKIPALEDLETAWLSFLT
                     PNAGGNQFYCLPDVGELVAMLLDAQGEGGCVFGAIYNEQDPTPVADSDMWVKKFSNGT
                     EISHNRKTGDVVVKTNGHLTATAQGGATINANTVVNGTLHATGKITSDEEVSAPVVKQ
                     GTVELGTHKHGGSPEPNK"
     gene            27767..28129
                     /locus_tag="PXD71_000197"
     CDS             27767..28129
                     /locus_tag="PXD71_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL6290366.1"
                     /translation="MNRQTSIQLTHWQLAPSTDTVEVVQGIDDIHLCIVNILSTQKGT
                     DILRPEFGSDHFRYIDYPEDVAIPNFVREITTALQKWEKRIEVEEVLVDGSAPHFIFT
                     VMWHLTDDVYREIYRTTV"
     gene            28139..29242
                     /locus_tag="PXD71_000198"
     CDS             28139..29242
                     /locus_tag="PXD71_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL6290367.1"
                     /translation="MTEAIKIIDDDVKTVLAEAIADYEKRTGKILQPAHIERSIIQTY
                     AYREQLLRKGINHAFLQTFPQFATGIALDLCGETFGCYRLRDKPARTLLRFSVSGEHT
                     AIVIPKGTQVAINDELYFSTLNDDVITPLITYVEIDAECNQVGVIGNGWESGRVSKLK
                     TALATNYKVTVENIDITSGGLMQEEDEPYRKRILAAPEAFSNCGSIAAYDYHVRSVSQ
                     DIADVNISTPRGGLVRIAVLTQKGLPDTRLLDDIKKHVSAERLRPLCDMVEVIKPTER
                     RYQITARLILLDGYREDLVKAKAREALQLYLSDKTKKLGLDIVPSAIISALRVEGVYD
                     VNLIQPVKTIINETEWANCTAISVEVEEERRDG"
     gene            29235..29801
                     /locus_tag="PXD71_000199"
     CDS             29235..29801
                     /locus_tag="PXD71_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6290368.1"
                     /translation="MASLHYADIIVRDPKYKALADLSKRLNLLDTSQIMTTLVDLLGD
                     ECIPLLAEKWSVTGYDGLLVADSENSKRALIKRAIELHQLKGTPWSIREVLRRLGFGE
                     VEIDEGLKNRLYESTYVSGIPENERWAHYAIRLSNPITNEQARNIRKVLRNFAPARCS
                     LAVLDYKADPIRYNNKATYNGTYNHGSS"
     gene            29811..32090
                     /locus_tag="PXD71_000200"
     CDS             29811..32090
                     /locus_tag="PXD71_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDL6290369.1"
                     /translation="MAGVQETAQWENHVYRIEENDPVHGGENGVTNKPIKHLANRTLY
                     LRRLLTEAGQRINPKKITATSKNSNDLTGHTHEIDHASLTQKGIVQLNASIQSTSTTE
                     SATPSAVKAAYDKAATAEQKATLANEKVEAIKVGGRNLLIQSNTPHNGNAYSVRYALA
                     EAPKVGEEIVVTLWGELGSQRTGIGVYNSQGATELAKLVKIRDGVYQGKGIWRKPMSE
                     GRERTPNDTHLNVYFYPKTATDNNRIERIQLERGSIGTDWTPNEADLKAEISAIRTKL
                     IPVRGGNVATTLNAAKRSDWFREMQIPYQSGWGYYTQAFHNSGTQHNYSNLPLTGRYY
                     FIAELYHNSGYSHLRITYPSLKRTFESAINLNNEDLILDWREVVFLKDGLYQGTFKTT
                     GTLESAYRLRAARDDERYQPYLQMVDMGYDLANPPVGKNKIIGSVDYYVKNGTSESSK
                     ATMMAAAMPDKNVFWEVALWNAAQQRNVLFKGYSKTNNLSIGKTTDNERDRVQVNGTI
                     QATAPADNANNDQVPTTAWVRKMAKILADGKVSKAGDTMTGHLFIKNGEYSAVHTYNT
                     SGWFAKWEAAPLSDSHFAAIVYANDRETVINRVLIPKKNGTVALINDIADTVRAAVSR
                     SDKSRSRTFPLQTGKKYTTTGSVTIYPDGRIVQIFHLKNIKAAWFDYEASAVGDRHRV
                     VNIPLWTAMPNKIFDVDVKTVRPSNNASTYYIEAAEWLSAWQIYGNDTNKSSVNINLS
                     RFRGGKDEDMDLYVTVEGY"
     gene            32091..32693
                     /locus_tag="PXD71_000201"
     CDS             32091..32693
                     /locus_tag="PXD71_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290370.1"
                     /translation="MAYFVNVIDEQGNYELIDDEFIGLYPDLDFNTLPKLTDKQYQTY
                     LAKANGKERFVNGEFVYEQIKVDMQAVISAEKSAKLAEINQKAQAFINDLAKYNETPK
                     FERDTWLEQAKEAKAWVADPTVQTPTLELIAQMRGIPLDTLRQKAYEKAMAYQTVAAI
                     VAGQRQGYEDRLEQAETLEQIQAIEPMYQLPQGANNEQTD"
     gene            32677..32952
                     /locus_tag="PXD71_000202"
     CDS             32677..32952
                     /locus_tag="PXD71_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290371.1"
                     /translation="MSKLTKLKSWSYHVLIAIDQLFNALTGGAADETFSSRCYRGAVL
                     AENPKKRWRFWHSFLNCLFLDPKHCYAAYKAEVHRKQYPKAFRLGGI"
     gene            32952..33239
                     /locus_tag="PXD71_000203"
     CDS             32952..33239
                     /locus_tag="PXD71_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290372.1"
                     /translation="MKKQNLKLVRGDDVSFRFSIKDSTGQPLALKDLWFDLHLVSYQG
                     EVLIALSTDDGSIETAEAGQMVLHFSHQQTARAEWTSANYDLQLTHDYTRV"
     gene            33249..33413
                     /locus_tag="PXD71_000204"
     CDS             33249..33413
                     /locus_tag="PXD71_000204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290373.1"
                     /translation="MTSIIELDLTDDLIIEVQLDSTIITGDSFLANLPDLLQIYNQAK
                     SDSSKELNND"
     gene            33406..34110
                     /locus_tag="PXD71_000205"
     CDS             33406..34110
                     /locus_tag="PXD71_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290374.1"
                     /translation="MTKHKNHENHPIAITFYQLGKDVGDINRRLAVIEQREQSAQPSQ
                     PQAEAVTMPQQDAVAINNINAVLEAVEKYKTFEKYNTQWQERFEKFLSRNPNDYELSE
                     EQRQKLYHTMCQHLWQVNFTEINSKQYELPIPQQFQGKFGYIVKTSGGSTEISFELLS
                     ETHSTYLYPDSYIPEGGGDVMLKPAYIYLVIPDLVAMREPEVVGVSPLKLNILQKHLL
                     TPQKLQEQKPLNTKAN"
     gene            34279..35262
                     /locus_tag="PXD71_000206"
     CDS             34279..35262
                     /locus_tag="PXD71_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290375.1"
                     /translation="MNKSLVALAVAAMASKAQTKQQASKQASKQASKQASKQASKQAS
                     KHCEIEKVFKQAPLPFSGQKRMFLSHFKQVLNANIEADGKDWTIIDVFGGSGLLAHTA
                     KREKPLARVIYNDFDNYAERLNHIKETNQLRQEIYQIVDEIIPKNKRISNEIKAKIIN
                     KINDFEGFKDLKCLSSWLLFSGEQVATLDELFKHDFWHCVRQSDYPEATGYLDDIEVV
                     SESFHQLLPRFHDKEKVLLILDPPYLCTRQESYKQENYFDLIDFLRLIDLTKPPYIFF
                     SSTKSEFIRFIHYMVQNRKENWQAFEGAERIVVNASASYNGKYEDNMVYKF"
     gene            complement(35299..37737)
                     /locus_tag="PXD71_000207"
     CDS             complement(35299..37737)
                     /locus_tag="PXD71_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family igA-specific metalloendopeptidase"
                     /protein_id="HDL6290376.1"
                     /translation="MKHTFKLSLLATSIAISLSPTLLASIVRGDVDYQYFRDLAENKG
                     KFFVGATNIPVIDKTGKNIGTFLQVTPTKQVEVESNISPKPNVSLSECFDNESLPGCY
                     EFLYGGGDEDSNDLPVFETVTGETMSIPMIDFSSITRQTGIATLVNPQYVISVNHNQG
                     YRDIQFGDSSSEKADDHHYNYSVVRRNNYMPDPNKERTDLGKGIEKRERNDEGEKLIL
                     DGSGNPTPRWDYHAPRLSKLVTEVAPANEIERTTENVQDFYSVFSDPFIFPMFIRAGS
                     GRQAVVNNERDRLNNGRIEVGNGPFLTGGSVLPVTNGDPSVPGSDFNSRILVAKTENN
                     TINDVFKDHGYGPLTTLGLPGDSGSALFGYDVRTKKWVVLGVYSDYFSENNTLGGDVY
                     KSYWNYHHPHYVRALEKENNAGAINANGARLTWTPSGNTSSIVGGNAPLTVNLADTSL
                     PTLQGHNGNTGDNPWNPQLQHGKTFHILGENNTLSLTENINQGAGAIHFHGNTTVEGM
                     KAGITWLGAGVDIDKDKNVIWKISNPAGDRLSKIGQGTLTINGKGENKGSVSVGDGTV
                     ILNQQADENNKKSAFSELGIVSGRPTVILNSSDQMDPNNIYFGYRGGRLDVNGNDLTF
                     RRIQNSDEGAMVVNHNTAQTANITLTGIVSPTIDQVKVQAITSPDQVSRHLDKDIFTY
                     QRFPHLPTNFIRLKKPLSVAEITAIFNARKVFIDLDFATGLIRENEYFEVLKVTDESA
                     AKQQILAEKIKAAEGTVQGFNGYFGETDPSKTNGRLNITMNSNCSEPTKLRNATTKTT
                     KENRSFTFLILMVF"
BASE COUNT        11900 a         7520 c         8802 g         9650 t
ORIGIN      
        1 atttacatca tttaagtagt ttctaaaact aactttcaag gatctcacaa aatggcaaga
       61 aataaaagag ttcgagatat gagtaatttc gaaattcgtg gtgagctgat gaaaatcggt
      121 aagtccttat ctcagttagg tattgagaat ggattagcaa aaaccacagt tcgtaatgcg
      181 ttagataagc catatcccaa aggggagcaa attattgcaa aagcgattgg taaaacaccg
      241 caggaaattt ggccctctcg ttacgaataa ggagctttta tgaaggaatg gtttttagcc
      301 aaagaaatcg caggattatc tggagttcca gaattaccga atagcgtttc aagattggca
      361 accaaagaag gatggcaaaa acgacaaatt caaggtgtaa gaggtgttac ttacgaatac
      421 cacctcacca gcctacccat tgaaacccaa caacaactta gactaaacgc cgcccttgcg
      481 gtaataccgc aagcggcaga actacaaccc aaacgagacg accccgcact tatcgcaagg
      541 ctcaataatg ccaccgataa aggcagggat aaagcaaaag gcaaggcaga agcctgtatg
      601 cagttgcaag cctttctcga ccaagggttt agttatacgc aggcagaagc gggagcggca
      661 accgctaaaa atgtgtcgca aggttcttta aaaaattggt actacaaagt gaaaggtcac
      721 cccgtccatt tatggcaggc gatcttaatt tccgaaagcg gtaggagtaa aaagccgcag
      781 ctgaaagcca agattactga agaagcgtgg gactgctttt tggcggatta cctccgccct
      841 gaaaagccgg atttacgggc aagttaccgc cgaacccaag ccatcgccaa gcaatacggc
      901 tggcaaatag caagtttaca gactttccaa cgccgagtgt tggcggaagt gccttacgag
      961 gtgattttgc tcaaacggga aggagcaaat gcggttgcca agttagtgcc ggcactacaa
     1021 cgcacggtga aagatattct cgcaggagaa tggattaacg gcgatggcta tcaacacaat
     1081 gtgtttgtga aatggcacac cggcgagatt gtccgcccta aaacgtggtt ttggcaagat
     1141 gttcgcaccc gcaagatttt aggttatcga acatcgattt cggagaatac tgacagcata
     1201 cgccacgcat taatggatgt gatttttaac gtaggcatac cgaaaacgct cacgctggat
     1261 aacacccgag cagcagcgaa taaggcaatg accggtggta ttgaaaaccg ctaccgcttt
     1321 aaacataccg agttagaccc gaaaggcatt atgccgattt tagggattga tgtgcatttt
     1381 acctcaattc tttatggtga aggacacggg caagccaagc cgattgaacg tgctttcggg
     1441 cgaggcggaa ttggcgagaa aatagataaa cgccctgaat taagtggttt ctatacgggc
     1501 agaaatgcac aagaaacccc tgataactat aacggcggta aagacggtgt ggattacaac
     1561 acattcttga aagccatagc agcagggata gaagagtaca actctcaaac cgaacgccat
     1621 accgaaatgt gccgaggaga attgagtttt aaccaagttt gggagcggga ttaccaccca
     1681 agcaacgtaa gacaagccag ccctgaacag ttgcggttac tgttcttaca ggcagaaacg
     1741 gtcagcatta aacgcaacgg cagttttacg ctgaaagcag ccggcaaact ttacggctta
     1801 actaacgttt actgggcaga aagcctgata gggataacgg ataagaaagt agtggcacgg
     1861 ttcgaccccg atgacttaca cggcaacgtg tatgtgtaca acctagaggg gcaattctta
     1921 gccgaagcgg tttgccgaga ggcgaaagga tttggtgata ccagtgcaag ccgtgagcaa
     1981 ggtcgcttat atcagaaagt agtgaaaagt gccaaagctc aagccgaagc ccttgaatta
     2041 ctggaagctc acgaactggc aagcctcgcc ccacaagtgg acgtgcctga gccgatagag
     2101 aagaaggtaa aagaggtgct ggtgaaagaa gattttatcg tggatttcaa cactgtgcgt
     2161 aaagcaacgg tggtggaaga aaccgaagag atcagtattt ttgatgaagt gttactgaaa
     2221 caagagaaac aacttcgcat agtggaataa ctagcggtca gtttttgcaa aaaatttgca
     2281 aatttttcta aaaaaacgac cgcttgaaat gagatttaaa cagggtttaa acgcctttaa
     2341 ggagtaaaaa atgacattaa cagggcaagc ttaccgagtg attttcaagg aactcaaaga
     2401 gcaacaagag agtgaatttg aacaggcttt caagaaaggg attgccaaaa ttcagcagaa
     2461 tcaaactcag gaatccattc tggtaaaccg tgatatgttt caagctgaaa agctaacact
     2521 gctttcacaa aagttggaag cggttgagca attacttcgc cttgaattaa atcagccgaa
     2581 ttttcaagca aaacttgaag actacgctga cgcattacga acaaccattt taagtaaagt
     2641 agccaaataa gagctttaga ctcttgaagg cttaacatct gagccacttt ttttactgtc
     2701 ggcattatac cgtcaagatc atcaatagaa gttaaatgat gaaaagagga atggtattca
     2761 gctaaacaaa aattgattgt actaagttta aacaaagtaa tgcaattttt catcacgatg
     2821 gcatttgctc gttttaactt aaaaccagag aaggttgcac gagcagaatc aagggctatt
     2881 tgatgaattt catcatctgt taaagaacga aagagcataa aaacctccaa acaaaaacag
     2941 gacaccatta tgacattaat cgaccaaatt aaacaacaca tcgccgaaag cggcagttct
     3001 caggggaaag ttgccaaaga agccgggatt aatgccgggg cgttatcggc ttacctgaat
     3061 gaaaattaca aaggcaataa tgaagaactt gaagcaaagc tgattgctta tttagagcgt
     3121 attgaggcga agaaaagaga gtttgtcgaa gctccgagtt ttattgaaac caaaaccgca
     3181 aaccaaattt tcggttcgtt acgatttgct caaaatactg gcgtgcttgc cattattcac
     3241 ggtgcgagtg gcgtgggtaa aacacaagcg gctagagagt atcgtaaacg ctatgcgaat
     3301 gtatggttgg ttaccgcaag cccttctcgt tcctcattaa gtgaggtgtt atatgaaatt
     3361 gctcttgaac ttggaatgaa cgatgcacca cgccgcaaag ggacattggc tcgtttaatt
     3421 gttcgcaaaa tgaaaggcac agcaggttta ttaattattg atgaagcaga ccatttacct
     3481 tacgaagcct tagaagaatt aaggctgtta caagaagaag tagtgaatga aagtgacatc
     3541 ggagctggtg ttggcttagt gctgattggc aatgacaaag tctacacccg aatgaaaggc
     3601 ggaattaacc ctgctcacga atatgggcga ctttggtcac gcagtgccaa acgcaccagt
     3661 attcagaaaa ccaaaaaaga agatacccaa gcggtagcca aagcgtgggg gttgagtgaa
     3721 aaccaagaag cactcaaggt aatgcaagcc attactgaaa ctggtggcgg attacggatt
     3781 ctgacacaca ccttacgatt agcagcgatg gtagcaaaag accgaatgat tcccttaact
     3841 gccgatttaa ttcagctcgc acgtaaagat ttactctcaa caggagatta acaatgagaa
     3901 ccccaaaacc caaacacagt tttaaccgca ccaaccaagc agcgtgccgt tttttaaaac
     3961 aaacccaaaa ggcaattatc cgcttaaaca gtcttggctt tacggtgctg aatattgatt
     4021 ttacccgtat taagccccgc attgaggtgg aaatcggcaa taacaagcac attgctcaag
     4081 gcttgattta tgagggtaaa gcctaccgct acagcttcgg taaaagcgaa gatttaggca
     4141 gatgggaagg ctactacacc atgctggaag gtatcagagt gtggtggcga caagcagcaa
     4201 ttcactagga gaaaaacaga tgaaaacctt agcactatta accgcccttt tagggcttgt
     4261 agggtgtgat ttagtccaaa gcaaacaagc taaaaattac atcggcaaca atttaactga
     4321 aatctgtata gataacgtgg tgtacctgat ttattccggt gataaaaaag gcggaattac
     4381 cccgaaagta aataaagatt tttaccctta tacctgtaca aacaaagagg aaagacaaaa
     4441 tggcaaaacc cgatgaaaca cccattacaa aagcagaatg tcaatctcag cttgcagagt
     4501 tgggggtgca atataaaaaa ctgccaatgg caattacaag acatatttgt aatgccacaa
     4561 ccaatataca cggcaaagtt ataaaggtca gtgtggtaga gagagttgga tatggcgtcc
     4621 agattaccgc acaaggtaac gagaaatctt gcttagtaac ctatgaagca atgttaggta
     4681 tggcagaagc aatgggatta tttgatgaag ttaaggagca aaataatgac taaaaaaaca
     4741 accagagtaa aaagtgcaac ccaaagtgcg atttatcaaa gccgagatga agtgcaagtg
     4801 gctataaaaa tcattggtga taaacagcgt gaattgcaac gtttagctac ggcaatgaat
     4861 gatgaattag ctgctattag tgccagttat gctcaacata accgctacca acattatgcc
     4921 gagaaagcgg ctgaagcaga acgcaaaggc aactataagg aagctcaaga ccattgggaa
     4981 gttgcaaaat tatcagcaaa aaagaccgct aaccgagact gggcggaaca acgagcggaa
     5041 ttttgtaaac gtatgcacaa taaaccgttt tagggggaag tgatgacaga aaccgtaaaa
     5101 gcacaactta acagccaatt aaatgaggca attatccagt tgatacaagc tcaaaagtat
     5161 ttaaaccaag atgatgccat tcgcagtgga gtgtatatcg gtacggtgca ggatttattg
     5221 ccaaaggtgc atttaaagtt attaacggca aatcgtaaac attgatagag atgaggtaaa
     5281 aatggctatt acagaagaac aatggaaaga aattaagcaa aagctagata gtatcatagg
     5341 gagagttaaa tttagataca aagagcattt actcacggta gatgttgtac aaattaagcg
     5401 atctttaaaa ttggctgtgt atgttgatgg ggaaattgat ggagcttgga caaaagaagg
     5461 acacgaaatt cgcccttatt tagaagaagt ttggtatcgc aaagaacgcc ctttttttaa
     5521 tgctaaggag aagaaagagt atagaggttt aatgagcaaa aaaaaactga atgaaaaaat
     5581 agtaacctac tcacctatgt ttccatcacc aactgcactg actcgtcaat ataagaaact
     5641 agatggctta gagttgattg aagtgattta aaacccattt acagcccatt taaccccgag
     5701 ttatttaaag gacaacaaaa tgaataaaaa cgaatttttt aagaaatacc acctagaggg
     5761 ttcactagct attttggatt tggatgcaaa tgacgatcaa agccaattct gttaccaagc
     5821 attgttggag tatagagaac tcccgttaga cacatttctt gcagagtact cagatgatga
     5881 ggatatgaca gagtcagagt ttattgagtt aatgcttgat gaattagagt ggattatccg
     5941 cagagctaag aatgctaaac ggtttgttaa aaaacgacaa aaaacgatga gggaggaaat
     6001 tatgtaacag ctcaacccaa ccaacgccac atcatccgtc tagctgagaa acaggtgtgg
     6061 cgtttgtttt aagtgggcaa tcaaaagcga taacgttttt aattgtttat ttatcggagt
     6121 aacaaatgac agacaaagcc aagctaatcc agcttatcca tattgccaag tcgcaactag
     6181 caatggacga tttaagctac cgtgaaatgc tgaaacggct caccaataaa accagctcga
     6241 ccaaatgcac ggtggtcgag ttgcacaaag tcttacacga actgcaaacc aaaggagcaa
     6301 aagtaaaata ttttgccaaa cgcagcaaaa aaccgaccgc ttacagcccg gcaacagggg
     6361 aaacagcggt aaaaagccaa attgctcata aaatccgagc ggtatggata acaatgggca
     6421 aacacggatt tttaaacgac ccaagcgaaa aagcattaaa tgcttatatg cgtaaggtta
     6481 tcaataataa aactagacca acaatattat taaatgtctc tgcattagag gctggagatg
     6541 caagttatat gcttgaaatc ctcaaaaaat ggcataaacg tgtgatgatt aaagccctaa
     6601 gcaaaagttg cacagttgat ccaaagataa gctacgatga attagtggag tggtacaatg
     6661 aagttatgta gatgcccgat ttgccacagt gatatacacc tagaagctct cgtagaagat
     6721 gaagccgggc gtgaactact cggcaaaatc agccaattaa cacacggtgt tgctcaaccg
     6781 atggttgctt atttaggctt atttaaaccg caaaaaagta accttaataa ctcaagagcc
     6841 ttgaaaatac tcaatgatgt attagcactc tacccttgct cattgctact ggctcaagcc
     6901 ctatctgaaa cggtagtaag catacgcaaa aaacgtcagc aagcaatgga aaacgggcag
     6961 aaaattgaac cgctaccgaa ccataactat ttaaaatcgg tgtatgaaac gcaaaaagtc
     7021 cattttgctg tggtgagaac cggcaaaacc gaacgcgaaa caatgaaagc cgaactggaa
     7081 aaaacacgca atgcgattgc ctatgtgcaa cggtttgttg atttaggacg tgaagaggtt
     7141 gttaaaaata gccctgagta tcagatttgg ttagctcata aacccaaata acacaagcca
     7201 ccaaaaggtg gctttttaac tttttgaaag tgcgataaaa cctgatttaa acaggcttta
     7261 aacgggtttt aagattgagt ttaaaaaaat atttcacaaa ataagaatgg ttttttatac
     7321 aataaccgaa acggtttttg gataggaaat agacaatgga attagtggat atttttgaag
     7381 aaaaagcccc ggaagtgtta accgatttag ccaaacatat tgaagtcgca ttagaagaaa
     7441 aatgccaatt tagccccgaa aaggcaaagc aaatcggtat tgatattgcc caacaaatgg
     7501 ctcaaaactg gggtggcgag gtaatttata ttcctcgcaa tttgttgatg gctcttaatg
     7561 agcgagatat gaaaatttac gaagagttta acggtaacaa tcaccgtgaa ttggctcgta
     7621 agtacaatgt atcaatgcag tgggtatata aaattgtgaa gaaagtccat aaggacgaag
     7681 ttgcccgcag gcagatgagt atgtttgaga tgtaaatcat tttttcccag ccctgatcat
     7741 tttcagggct ttttgtgtat taaaagaacg cagtctttaa acccgtttaa aatcaatttt
     7801 tcagcaaatt ctttaaactc cttttaaagc aattttaata ggagtgtgct atgtctcaaa
     7861 atcttacctt tcgccaaatt tttgaccgtt taatcggaca cgagggcggc tatgtcaatc
     7921 acccacaaga cccgggcgga gaaacccact ggggaattac caaacgtacc gcggttgcta
     7981 atggttatat gtatgcaatg aagaatatga cccgtgagca agcctaccaa atctacgaaa
     8041 aagccttctg gcaacgttat cgttgtgcag agcttaagcc agcagttgct tatcaatttt
     8101 tcgacgcagt ggttaatcac ggttttggta atgctagtcg tatgttacag cgtgcagtag
     8161 gcgtagcgga tgatggcatt attggcaata ttaccctgca ggcaattaat gcaaaatctg
     8221 aaagcgatct cttaatgcta tttaatgccg aacgtctgaa gttttacaca aaattacgca
     8281 acttccccac ttttggtaag ggttgggtca atcgtgtggc aggcaattta gaatacgcag
     8341 cacaagataa tgaggtttaa aaatggcaaa actatttaaa gaactactct caaccaatgg
     8401 cacattatcc accactagca ccgtgcagtt ttttggcaca ttgtgtgtat ttggtttaat
     8461 ggtttatgca gtaattaccc aacaacctta tgccgaaagc ctattaaata atgtgttgat
     8521 ttatcttttt ggggcaacta ccgctaaggg tgtagtaacc agctatcaag ccaaaattaa
     8581 agggggtatc cataaacaac aaagtacatt taaagaggag gagggatgtt aatgcaacaa
     8641 attttaggcg tgattggcgt catcgcagca atattgtctt atgctttgtt taaaagctgg
     8701 caactaaaac gtgagcgtaa aaagaatgcc atattacaag ctgaaaaaca gcaacaagcg
     8761 gttgaaattg agcaaaaaaa agcagaggta aaatatgtgt atatatcaaa gaaaaacaat
     8821 gaaaaagtta ctcgcagctc tgctagcgat attgatgaca agttgcagca acacggttac
     8881 ttccgtgaca acaacaggtt gcacggcgtt cagtctgatt tatccgaacc gtgcggatac
     8941 gctggaaacg aaacgtcaga ttcttgctca taatctgact tttgaggaaa tttgccaaaa
     9001 tgaaatacat taaaaaatta cgtaaacgct ggcaaatttg gcgtttttta aaaaatcatc
     9061 ctgaggttgc acaacgccga gatttattag cgattgccat taaacagggc gtagaaaacc
     9121 ctgtagggcg tgcaagacgg agggagccat aatggattta tttacagtct tttgggagtc
     9181 tatccgtgct aactttggtt ttatttctgc gttactcacc attgttgtta cggcattttg
     9241 gctcaagcta gatagcaaat acgccaagaa aaatgatctt ggtgaattgc tggaaattgc
     9301 caaaagccac gataaccgct taaccacctt agaaaccaag gtagaaaacc tgccaagtgc
     9361 ggtggacgtg gaaaagctga aaaccttagt cactgatgta aaaggcgata ccaaagccac
     9421 ctcaaagcag gtggatagta tcagccacca actcggcttg ttattagaag caaaattaaa
     9481 ggaatgatat gtcgttaaga gatttactca ctcaagacca acggctggtg attttacgct
     9541 cattagcgga agccggctat gatgccaatg agtcaattat tagtgattgc ctagatttat
     9601 acggtcacga tattagccgt gatttagttc gcacccattt aagttggctt gaagagcaag
     9661 gcttacttac cttagaacgt ttgcgagatg gctatatggt ggcaagcatt acccagcgtg
     9721 gcttagatgt tgctcaaggg cgtgttgtgg taagtggcgt taaacgccct cgccctaaaa
     9781 tttaaacggt atttaaggag cgtttaaatg agcgagaaaa atactcgtgg tcgagcatca
     9841 aaagtcgatt tactgccgcc gaatattaaa acccaactgg cgatgatgtt gcgtgataag
     9901 cagtactctc agtcacaaat tttagaagaa attaatgact taattcggga ttgtggcttg
     9961 cctgatgaaa tgctgttaag caaaaccgga ttaaaccgct acgcaagccg tatggaacgc
    10021 ttaggggcaa aaatccgtaa tgcccgggaa gttgccgaaa tttggactaa gcaattcggc
    10081 gaaatgccac aaaccgatat tggcaaaacc ctaatggaaa tggtcaagca aattgccttt
    10141 gaaacctcat tgaaattagg cgaacaagaa ggcggtattg agccaaaaca gcttgctttg
    10201 ctctcgtctg ctattcaacg tttagaacaa gccgaaagtt tgagttttaa acgggaacag
    10261 gcaatccgta aagaggtcgc acaacaagcc gctgaaaccg ctgaaaaagt ggtggtgcaa
    10321 gcgggtttat ccgctgaaac cgtccgtacg attaaagagc aaattttagg aattgcataa
    10381 tggcactact caataacaga ccgctgaatg aattagcccc tgaatgccgt tcgtttttag
    10441 agagcattca cgcttttaat cctaacgaac tgttattggg ctatcaaaag cggtggattg
    10501 cggacgacag ccaactcaaa attgccgaaa aaacccgccg ttgcggttta acgtgggcag
    10561 aagcagcaga taacgcctta atcgcaagca cccgcaaatc agacggtggc tctgatgtat
    10621 tttacattgg ctcaaataaa gagatggcac gtgaatatat cgatgctgtc gcaatgtggg
    10681 caaaagcctt taatcgtgtg gctggagaaa tccaagaaga gatctttgaa gacgaagata
    10741 aagacatctt aacctatgta atctacttct catcgggttt taaggtcaag gcattatcca
    10801 gtaatccgaa aaacctgcgt ggtatgcagg gcgtggtggt gattgatgag gcggcattcc
    10861 acgaatactt agcggaagtc ttgaaggcgg cgttagctct cacaatgtgg ggggcgaaag
    10921 tccgtattat ttcgacccac aacggctcgg aaaatctgtt taatgaactg attaccgata
    10981 gccgtgccgg ccgcaaacgc tactctgtgc atacgattac gattgaagat gcctgccgtg
    11041 atgggctata tcaacgtatc tgccaagtag tgacgaaaaa aacgtggtca ccggaagcag
    11101 aaaaagagtg gattgaaaac ctactcaacg acaccgccag cgaagaagac gcattggaag
    11161 aatacttctg cgtaccgaag ttcggctcag gcttatggct ctctcgtgct ttgattgaac
    11221 ggcagatgag cgaagccacg cctgttatcc gctttacggc aaaagatggt ttcagccttg
    11281 tggctgaacc gacacgctat gcggagattg ccgaatggtg tgaattagaa cttgagccga
    11341 tattggcaac ccttaaccct gatttgttgc attttatggg cgaagacttt gcacgcagcg
    11401 gcgatatgac ctctctggtg gtgcttgctc agcaacaaaa ccttgttcgt aaagtcgcct
    11461 ttattgttga gcttggcaat atgccctaca agcaacaaga gcaaatcgtg atgtttatgc
    11521 ttaaacgctt accgcatttt gccggtgggg cttttgacgc ccgaggcaat ggtggctatt
    11581 tagccgaagc tgcacgagat gaatttagca ccttgattga ctgcgtgcag ttatcggaaa
    11641 aatggtatcg ggaacacacc gcccccttta aagcggcatt ggaagacggc gagctggaag
    11701 ctattccaaa agatgcggat attttggctg atttgcgttc atttcaggtg gtgaaaggcg
    11761 taccacgcat accggataaa cgcactaaaa gtgcagatgg gaaaagcaaa cgacacggtg
    11821 ataccgcaat ttctttgctg ttggctcatt atgccagccg acaattagtg caaatgccag
    11881 ttgagccggt atcacgccgt ccacgccata gccaacaatt aacagaagga tattaacaaa
    11941 tgagatatgt accactttgt ttaatgatgt gtgcctcatt ctacctattt attaacaaca
    12001 ttgacggctg gggttgggga attcttttat cttttatttt agcgagtgca gcttatggca
    12061 aagaagaata atttaattag tgaaattgcc acccgtgcca gaagtattga ttactgggca
    12121 tttggctatt atctgccgaa ccctgacccg attttaaaac gaatggggaa agatatttcc
    12181 gtttaccgtg agttgttatc agacgggcaa gttcgctcag gcgtacgccg tcgaaaagcg
    12241 gcgattaaag gcttagagtg gcgaattacc accactaaca atgagaaagt ggacgagcag
    12301 atttatcaaa tatttaaccg cttgccgatg aataacctga ttaccgaaat gctcaatgca
    12361 gcactctatg gctaccatat cagcgaggtt atttgggcaa aagagggtga gttatttgtg
    12421 ccaacagaaa tcattggtaa aaagcctgaa tggtttatgt ttgacgacga aaacctactg
    12481 cgtttccgta ccaaagaaaa ctgggcggac ggcgaattat taccagatga taagttcttg
    12541 cttactacgc aagaagccac ccaagataat ccctacggtt taggcgattt atccctctgc
    12601 ttttgggcgg cgacctttaa aaaaggcggt ttcaaatact ggttggaatt taccgaaaaa
    12661 tacggctcac cgtggttgat tggcaaacac cctcgccaag ctcacaatac agataaagag
    12721 cgactagctg atgccctaga gggaatgatt agctcagcga ttgcggttat ccctgaagat
    12781 agttcggtcg aaatcaaaga tgcagcgggc aaatctgcca gcaccgatgc ctttgaaaag
    12841 ttcctcaatt tttgcaaggc agagatcaat atcgccctac ttgggcaaaa ccaaaccacc
    12901 gagcaagaaa gcaaccgtgc cagtgcacaa gtaggcttgg aagttatgga agatattcgc
    12961 tcggacgacc aagcgatgat tgaagccacc ttcaacacct tgttgcagtg gattgtgaaa
    13021 tataacttca atgtggaaca actgccgaag tttgaatttt acgagcaaga agccatcaac
    13081 accgaccaag ttgaacgaga cagtaaactc cacgcaatgg gcgtgcgttt ttccaaagcc
    13141 tactttgagc gtgaatatgg ctttgaggcg ggtgatattg aggtgcaagc ggtcgaaaaa
    13201 gcggaaaaat ctgcaaatac cacagaattt agcgaaggac aacgccgaac ccaagtgcca
    13261 atgcctgtgc caattttgga acagctagaa cacgagggag aagcaatggt ggacgattgg
    13321 ctacaagggg tgagagataa actcgctcaa gcagatagtc tggaggattt ccaaagtcag
    13381 ctagatagcc ttatccctga attaagcttt gccgaatatg gcgagctgtt ggcgttaggc
    13441 tcaacggtgg cagagcttgc aggcagacaa tcggtaaacg atgaaattaa ggcaaaacgc
    13501 aatgcgtaaa tttaccttta aacagcaagc ccaatatttt gagaaaaaac tcaatctccg
    13561 cacgaatagc taccttgata ttctgggcga agagcacgac tattttttcg tggtggctgg
    13621 ggcgaatcgt aatgaagtgt tgagtgagtt tcgcaaggcg gtggatgagg ctattcacaa
    13681 cggcgaaacc ttagagaact ttcgcaagcg gtttgatgag gttgtggcaa aaacaggctg
    13741 ggagtacaac ggcggacgaa attggcgaac acggattatt tacgatacca atgtttacgg
    13801 ggcgtataac cgtggacgtt tgcaacaaca tttagagctt gcggaggata tgccttattg
    13861 ggaatatcaa cacaacgaca acgcccaccc tcgcccacag cacgtggcgt gggacggctt
    13921 ggtgctgcgt tatgacgatc cgttttggaa atatcactat ccaataaaag cctacggttg
    13981 ccactgcacc gtgcaagcgt tagatgattt tgacctcaag cacgatggta aaaccgtcag
    14041 cccaccacct gaaattgagt ttgaacaaaa aacggtaggc gtgcggacag gcaattcacg
    14101 agtcgtaact gtgccgaaag gggttgatgt gggttttaca ccgcataatt ttgataatct
    14161 caaagccggg agagcagcaa atgtagataa gttgctaatg caaaaaatgg ttaccgccga
    14221 tcctatattt gcctccaaag cagtcagtga tttattggca aaagagccgg ttgcaaagtt
    14281 acataaccaa gcctttaaaa attgggtaaa tgatgttgcc aatgctaaag gaagtgcatt
    14341 atcccaagta gagaatatgc agttgttagg ggtactacct gttgcggtga ttgaacggtt
    14401 acagcagatc aatttagctc cgcaatctgc aattattgca ataacaaaag gcgatatggt
    14461 acacgctcta cgagagctaa aacaaaatac cggtattgcc gtaccgttgg aattttggga
    14521 aaagttgcct gcaaagttac aatacccaaa tgccatcttg ttggaaaaac aacaagcaga
    14581 aactacattg ctctttatct atgacgtagc tcaaaccaaa attgccgtaa aagtcaatta
    14641 tgatgcaact ttacaagata gacacggagc aaaaggacgg cagaaagtct tactgaatcg
    14701 agcggttaca ggtagtttat tccaagatac aactggattg aaaagttcaa agtatgaagt
    14761 gctattggga gaaatgtagc ggcgaaggac tcgaacctta taatgtaggt taaacctaca
    14821 acctttacca attaggaaac taccgctaca ttgtgtaaat tcactatacc cctaaattat
    14881 tttttaatca ataggagaaa caaaatggca gaaaaacaac tttcctttaa agtagccgaa
    14941 atagtgccaa tgtcagctat taacactatg cggcacttaa ttagtttggc attacaagcg
    15001 aattatgaga gagaaaaatc tcgtgcttga taatttcaat ggcttgtagt tcgggaacta
    15061 tattatctga aacaagccat tgttttgttg atgtcagaca tttagcgatg aaatccataa
    15121 ttaaaccatt agcagtagct cgttgttccg gagtaagttg taaccacgac gcctctgttt
    15181 gttggtctaa tgtataaaga aacataacgg cttctttttc tgataatggg tcaagtggta
    15241 atttttcagc ttttgtgact aaccattgac gggaaagcaa taaatcataa accagttcta
    15301 ttgcttgttc tgcatttacc atcatattct aattccttaa tatatcaggg caacattacc
    15361 caatctatat tttactgaga tttgcttaat gatcaaaatc accctcaaca acacccaagc
    15421 ggtcaaatcg ttacaacaaa ttgcaaatca gctggaacaa cctcgccgac tgtatggcat
    15481 tttaggggaa accctaaaga aaattcacac tgcacgcttt aaagcggaac aagaccccga
    15541 tggcaacaat tggaaaccgt tagccagtag cacgcttgct ttaaaacgta aacgaggtaa
    15601 gtccactaaa atcttacggc aagacggcta ccttgccgat aaaacggctt acaatgttac
    15661 aagtgataat gttgagtttg gctcgcagga agtgtatgca cggttgcatc aattcggcgg
    15721 taaagccgga cgaggcaaaa aagtgactgt accggctcgc ccgtggttag gcgtaggcga
    15781 taaagatgag cagttacttt tacgcaaagc tgagtggcat ttagggcaaa ttatcggtcg
    15841 tatgggaaaa taaccgctta aaatcaaatt taacgcataa aaacgcctct gtgcgttttt
    15901 attttttatt cgcctgatta ttcgactcaa aaaatttaaa cgctgttaaa cgcatttaaa
    15961 cggtatttaa acggtattgc cgattgtgat aaatcctctt tattaaaaca ccctcgaaaa
    16021 atctttaaac cagtttaaaa gctaatttct ctccaaatcc ttattattgc cccatcacgg
    16081 aggcaatatg acccaaattg aaatttttaa agccggtaag cgttatgacg ccaacggcac
    16141 gttaattgac attacccctg aaatgttgca acagaccgtt gctgcctata accccgaatt
    16201 tcacgaagct ccgctggtga tcggacaccc gaaatccaac aaccctgcgt gggggtgggt
    16261 aaaagccctt tcccttgagg gcgatgtact aaaagcagaa gtagatcagc tagacgctga
    16321 gttttcagaa atggtggcaa gcggcaaatt caaaaaagtc tcggctgctt tttacttacc
    16381 tgatagcccg aataacccac ataaaggcgt gttatcgcta cgccacgtgg ggtttttagg
    16441 ggcaatgcca ccggcggtta aaggcttaaa gcaggtggaa tttgcagaag atgatgattt
    16501 tgtcgtcttt tctgaatggg gggttgcaag tctatttagt cgttttcgtg attggttggt
    16561 gagtgagttc gggcttgaaa aagccgataa agctcttccc ccacacgaaa ttgattggct
    16621 gaaagaagag gcaatgcgaa gccaaattag caaacaggta cacgccgaac aggcaacacc
    16681 tgaaccgatt tttaatgaac ccgcacaacc agaaggagaa cctgaaatga gtgcagaaga
    16741 gaaggaagag cttgaacgct taaaagcgga aaacgagcag ttgaaagccg aaaaagccca
    16801 agccgaagca gagaaaatgg aagcagagct ggaagctgaa aaagctggta atgcagagtt
    16861 ttgtgaaggt ttagtcaagc aaggcaaact tgccccggtg gcaaaagcgg cgtttgtgca
    16921 ggctttaaat aacctttcag tattaaaagc cggtcgtgag ccggaattta atgagggcga
    16981 agatgtggtt tcacagttta agtctgcaat gtcgcaatca ccacagatta tccaatttgg
    17041 agaatatgcc acagcagata aagccaaccc acaagaagcg gaagcagtgg aatatgctga
    17101 aggcacagat ccagtttcga ttgaagccga ccaaaaagtg cgagcttata tgaaagccca
    17161 caatgtaggc tacgctgcgg catttaatgc gatttatcgc taatgtttaa cccacaactg
    17221 aaaaggaata ctaatgacaa ctcataactt acaaaaactg cgtgtacaag accccgtttt
    17281 aaccgagctg gcacaaggct atcacaataa cgagctggtt gccgagacct taatgccgac
    17341 tgtcgaaatc gaaaaggaag caggcaaaat cccaactttc gggcgtttag ctttccgatt
    17401 gccttctacc gtgcgtaact tgcgtggttc atctaaccgt ttagatccag aagatattgg
    17461 ggcgattgat gtggcgttag aagaacacga tgtggaatat gccattgatt atcgtgaaga
    17521 gaacgaagcc attttttctt tacgtcaatt tgccttaaat accacccaag atgtgattgc
    17581 gttaggtcgt gaaaaagcgg ttgcaaccct cgccttagat gaaagccgtt atgaaacaga
    17641 caataaagtc acgctgtccg gcaccagtca attcagccat aaagatgctg atgtgtttaa
    17701 ggtatttgat accgctattc gtgccgtgaa gcgggctatc ggtcgtaaac cgaatgtgtg
    17761 tgtgatttcc ggtgatgtgt gggcagcgtt aaaagaacat ccggcagtgg ttgaaaagct
    17821 caaatacagt caagtagcga ttgttacccc tgaggtgttt gccaaactga tcggcattga
    17881 gaccgtaaaa atcggtgaag cggtgtatga agaaagcggt cagctcaaag atatttggag
    17941 caaagatatt gtggtggcgt atgttgctcc tcgttcgacc ggaggcaaag gcacggttta
    18001 cgagccgtct tatggttaca cggttcgccg tcaaaaaggc ttatttgttg atacttataa
    18061 agagagtggc ggcaaaattg aagtggtacg cacgaccgat attcacaaac ctcatttagt
    18121 gggtgcggca gcaggctatt taattaaagc tgccgtataa gccacatacc atagggtgcg
    18181 tttactaacg caccattatc tcaaacacat tacatagagg aaaaaacgat ggctaaattt
    18241 attgttttac ataccccaat tttacacgat ggcaaacgct atgaggtggg ggcagaaatt
    18301 gaactcaccg ctgaacaagc tgagaacaat gcgatcaact taaaagcggt agaagctgtt
    18361 ccaaaaccac ctaaaaatgc ctcaaaacct gaagatgagc caaaacccga aggtgaacct
    18421 aagcctgaag atgcagtgac ggaagatgat gtgaagctgg ctgaagcaaa aattgcagaa
    18481 gcagagcaac caaaagggaa taaaaaataa tgtatgtgca gattgatgag ctactcacgg
    18541 cgtttagtcg caagattgtg gtgcagctca gtaatgatga accgacagcg accgagcctg
    18601 atttaaagat gcttgaaacg gcagttaaag tggcaaatga gcgaattgat gcagcgttac
    18661 gctctcgcta taccttgccg ctggctgatg tgcctacgct tattaatcag cacgcattaa
    18721 cccttgcccg ttattggctt tatgcccgcc gtcctgaaac caaaatgccg gacaccgtga
    18781 aggaaaccta ccaacaagcg gtgaaagagt tggaacagat tgcaacaggc aaactgcatt
    18841 taggcattgc tgaaagcagt caaattttag aagacgatgt tttagccgat aacagtgaat
    18901 atgaagttcg ggcgaacgct cgcattgata ccagtcgtta ttaaagatta tgtcagccac
    18961 acaaccgatt ttagaagata tttgccaaca cttgcggcaa gcattgcccg agtgggaggt
    19021 ggaactcttc ccgaatgacc caagcaccta tcatctctcg cacattaacg gtgcggtgtt
    19081 gattagctat ttggcaagca aatttgagaa acctcgcact acacaagcgg tgctgcaggt
    19141 tcgccacgtt cagattgctt taacggtgct gacccgtgat ttgcataatg atgtcggggc
    19201 gttggatttg ctggataaat tgcggttatt ggttgtgggg tttaagcctc aaaactgcgg
    19261 tgagtgttgg ttgattgatg aattttttaa tggctcaagc gaagaaaccg ggctttggca
    19321 atatcagttg attttgcaaa ccgaaactca gcaggtgcaa caccaagcgg tcgaaaatct
    19381 gccaaaattt gtaaccgctc atcttcgccg tcaagaccag cctggttcgc ccggacttaa
    19441 aacttaaatc gtagggtgcg tttgctaacg caccatcaac atcacctgat tttacggtgc
    19501 gttggcaaac gcaccctaca aggagaatat tatgacgttt caccacggtt ctgaaaccga
    19561 acgagtaaac ggtggctctg tgccggtatc ggtggttgat agtgccatta tcggcattgt
    19621 cggtactgcc ccaatcggcg cagttaatga attgaccgtc tgtttaacca aaaaagattt
    19681 tgccaaattc ggcacgattt taaaccaagg cttcacctta cctgatgcct ttgatatttt
    19741 agctcgttac gcagcgggta aagtgtatgt ggtgaatgtg cttgaccctt cccgccaccg
    19801 tactaccgtg accgatgaag tcctcacact ggatcgcaat acgctgaccg ctcaaacagc
    19861 gaaaaccggc attattgcgg tgacgagagt gaaagacggt gcaaacgtgc tgaatgctga
    19921 tcggtattcg gtcgatctgc aaaccggcgt ggtgaccttt gtctctgcac cgtctgcccc
    19981 gaaaattagc tatatctatg ctgacccgag caaagtgacc gaagaggaca ttaaaggcgg
    20041 ggtggatagc ttaaccggta aacgcaaagg ttttgaacta ttgcgtgatg gttttaacct
    20101 ttacggtgcg gatgccaaaa tcttaatctg tccggaattt gataaaaccg caagctgtgc
    20161 cgctgccctt gccacccttg ccgaccaact gcacgctaag gcgtatattc aactgccgaa
    20221 agggacgagc ttatcaaaag ctattcaagg acgtggcccg gcagggacga ttaacgcctc
    20281 ggcaagcaat gagaacgtac gccatttctt cccgtatgcg ttggggtcaa gcaatactct
    20341 tgaaagcctt gcgacccacg ccgccggttt gcgtatgttg attgatgtgg agaagggcta
    20401 ctggttcagc acttccaata aagagctgaa aggggtgatc gggttggaaa ttccgctgac
    20461 ggctcgggtg gacgataagc aaagcgaaac taatctgctc aatgcggtag ggattaccac
    20521 cattttcaat agttttggca caggcttccg cttatggggc aaccgttcgt ctaacttccc
    20581 gactgtgacc catatcagca actttgaagt cgcctctcgc acaggcgata ttattgatga
    20641 aagcattcgc caagccgaat tgcagtttat tgatttaccg attgatgatg cgttgctgga
    20701 tagcttcctt gaaaccatcg acactttcct gcgtacgcag aaatcgttgg taggttattc
    20761 ggtgagctta gactatgaat atgacttggt tgatgcattc agccgtgggc aagtcccgat
    20821 tgtgtatgaa tacactccta aaattccggg tgaacgtatc agcaataaat ctgtgatgac
    20881 ccgtaaatac ttggcaaact tggtgtcgca acgataaggg gaaataaatg agtattgcaa
    20941 ttaaccaaat tgtaaacgga aatgtttaca ttaacggcaa cagccaaatg gggcgagtga
    21001 atgaggtcaa aatccccgat attgagtttg aatttatcga acacaaaggc cttggaatgc
    21061 acggtgtgat taaattacct gccggcacga atgcgattga agcagaaatc acttgggaca
    21121 gtttttatcc ggaagtgcgt gcggtaatgc ttaatccgta taaatacact cagttgcaaa
    21181 tccgctctaa cctgcaagtg tttaactcgc agggcttggc agccgaagag ccgatggtca
    21241 ccattatgaa tgtgtctgcc ggtaaaatcg gcggcacagg gcataaaaac aaagaaaatg
    21301 ccgaatttgc cgacacgtta aatgtacatt caattaaaca gaccgttgcc ggtcgtgagc
    21361 tgttatttat tgatgtgctg gcgaatatct accgtgtgaa cggggtggat gtgttgcaaa
    21421 aattccgcac caatatcggt tagtaaaatc tttaaagccg tttaaaatca atttaaacac
    21481 cgtttaagta aactcctttg tgaagttaaa caacaaccac tcacaaagga gtttttttat
    21541 ggctaacgca gctcaacaag tattaaccaa tcttcgtgta caaaccactt ataaattgca
    21601 ataccctatc gctttaccag atggtagagt tattgaaacc ttagatgtac gtcgtccaaa
    21661 aggtaaagat tttcgtttgc ttgatgaaaa agggtttgac cctgaaaaag atggcgtaaa
    21721 aattctacgc ttctatattc aacagctcac cacacttgtg ccggaagata ttgatgaact
    21781 cgatgctgcc gatattaagg gcttaaataa cctattggaa gatatgctta ttgagggaaa
    21841 gtccgacaaa taagtgaact agataccgtt tatgctgatc tcgcttggtg gtttaagttt
    21901 tcaccaagcg agcttttaga attggattta gtggatattc cgaaatgggt agaacagatg
    21961 aacaggcaaa taaaagccgg ttatgggcag attttacggt agagccaaat aaagggttcg
    22021 gtggctaaag taagtaaaat ggcgacgccc caagctagat acacaccaac ccacgccaag
    22081 attgtcaaaa tcaatccata aatgagtaca aaactaacgc cctcgcttag gctttgtata
    22141 gattgaatcg catctaacca gtagaagata ctccaaccga ctaatgctac cgctaacaac
    22201 catttagcgg tgcattctgt tcttgttaca caagcctcga aaccagccca ctcagtaatt
    22261 cgattattta atttagtcag catacaccac ctccttaaac tttccctatt ttacgctaag
    22321 gatttaacca atggcaaata atttagctat cggaatggta attggtgcaa gcctatcatc
    22381 aggttttcac ggtgcttttt caggagcaaa gaaaaagcta gaacaattag aacaggctgt
    22441 cgctaaaaat cagcaaagcc attctaaatt aggttctgag cttactgcat tgcgtagtaa
    22501 acaagctact ctctatgcag aaatgagccg agccagtagt aaaggtggtg ctgggcttgc
    22561 tcttatgcaa caggaatata aaaaaattgg caaagaaatt gccaatatta gccgagagca
    22621 acgcaaatgg agtacagaat tagaaaaatc tattgctaag caaactaaat tacgcaatgc
    22681 aatggaacgt catacacaag ctaaagaaaa ccgagacgag cttaaaggga aaataattgg
    22741 aacggtagct tctgcggcaa tgggtgtcgg cgtgatgaaa acctatatgg agcaagaaga
    22801 agctgcgaat aatcttaaaa tttcaatgct gaaagcagat ggttctttcg ggaaatttga
    22861 agagattggc aagattgctg acacattagg gacagattta ccgggaacaa aaaaagattt
    22921 ctacaagctc gcaatggcat taaagaaaca aggtgtttct gatgatgtgc tgaccggagg
    22981 ggcattaaaa acttctgccg aactgaatgt cttgcttgat atggatcagg aaagtggtgg
    23041 agagttttta gcgaagttta tggaatctca ccggctcaat gaaaatgagt taccacaagc
    23101 agccgattat ctgcaacgag caatgtttgc cggcggttta agtaaagagc agatgtatga
    23161 atcgatgaaa tactatgctc caaaactgaa ctcaatgaaa cttacagggg ctgagaatac
    23221 cgaaaaagtg ctggcgattg aagcaatggc aggtcagcaa ggcttagagg gttcaacctt
    23281 tggtacgggc ttaaatatga tgttaagccg tatgaataaa ggaccggaaa tgctcaaggc
    23341 agcagccaaa ggaatgaaag ccgaagctcg agagatgatg gaggctgtcg gagtagaatt
    23401 taatttctgg gataaaaacg gctcattcaa aggcattgat ggaatgctgg ctgaaatgac
    23461 gaaatttgaa aaaatccgca aaaaattcgg tgacaaaggt gtaggtttag ttgcagaaga
    23521 gctgtttggc attgaaggtg ggcgtcttgc tgatattttg gctatgaaag gtcaaaaagg
    23581 cttagatgaa atgctgagta aaatgcgtga acaagccagc cttcaagatc gcattaacct
    23641 taaaacaaat acattaggtg ctgccctaga atctttagga ggagcgtggg aaacctttgt
    23701 gggggatatt ggctctgtct ttgccgaaga tataaaaggg ttttcaaaag gcttgcaatc
    23761 tgcggtggaa tggcttgtgc catttgtatc aaaacataaa gaggcgatta aatttgtagg
    23821 tggtttggtt gctggacttg ttattttacg aggcgtttta caagctggtt cttttgttat
    23881 tcaaacggta actatggcaa tgaacgccct caaaattgca gcaatgacca atcctattgt
    23941 cttagtcatt ggagcaatcg cagcgggtgc ttacttgatt tatagccatt gggatagcat
    24001 tgccccttgg tttgcaaatc tttggcaaaa tgtgaccgct tacttcaatc aattttggac
    24061 ttgggtaaaa ggtgtttggt cgggcgtgac ggactgggtg tcttctgtgt ggaatggtgt
    24121 agccactttt ttcgggcagt tatggactac gattactagc ttatttagtg tagcttggac
    24181 aaatatccaa acaatatggg gaggagcaac agcttggttc tctggtttat gggatgggat
    24241 taagtctgcg gcttttggta tgttcgacag tattagccaa aaagtcagct ctgttgtagc
    24301 aagcattaag agctttttag gctttggcga tgatgtggat aaaatggggg caaaaattgg
    24361 caatgtagct acccaagtca tcagcaaaga acactcagga caactcagca aaaccgcaga
    24421 tatggcagcc aatacagggt tctctagcgg tggtttcacc gggcaaggcg gtaaatatac
    24481 ccctgccggt attgtgcata agggcgaata tgtgatgacg aaagaagcga cctctcgttt
    24541 gggtgttgga atgctggata gcttaaacta tggcggtgct atgggtttca ctcaacctgt
    24601 tttgcaaccg attttaggta agcaaaaagg cttttttggc tcactttggg acgatgtgaa
    24661 attcggggca aactttgtcg gcaatctgct tggcttaaat caaggcagcc tgaaaacgcc
    24721 tgattttaac ccaaatgcga ccaatgcaag cggtcaaaat tcgtcgattt ttagcaatta
    24781 tgaaccactc aaccgaaatg cggtaacaca gcaggaaagc tcgcaagcac cgcagatagt
    24841 cgtgaacttt aacccgacaa tcaatgtaaa cggtaatgct acgcaagagg ctcaatcgca
    24901 aattatgcag cagttgcaaa tgagtgctta tgagtttgag aaattgctca atcgtgtgct
    24961 agaccaacga agccgtagag cctattagga gtgatttatg tattttatgt taggcaatat
    25021 tgcctttgag ccggtgaatt taactgaatt tagcgagcaa cataccgccg aatttgccga
    25081 acacgctgtg ctaaaaggca aacctcgttt acaagcgatg ggcgaaaagc taaatgaact
    25141 taatttttct cttcggttgc accataccat aggcggtgta gagagccgtt atcaagcctt
    25201 gcttgaagcc aaagcgaagc aagcggcatt ggcgttaatt tggggcagtc gttataaggg
    25261 agattatgta attgtcgata tttcatcgac cacattgttc accgatggca gaggcaatgc
    25321 cctcgctcgt gaaatgcaaa tcagcctgaa agagtttgtg ggcgataatg acggtggtgt
    25381 gttgggtgag gcgttaaatt ttggtggtgg ctcactgctt ggctctattt tgccaagcgg
    25441 tgcggtaact accctatctc aaatcaaaac agcagtcagc cgaggtgtgg agctttataa
    25501 cagcggcaaa cgcttagttg acgaagtgca aaataccgtg gcgattattc ggcagttgaa
    25561 aaatgacccg gcaactgcat tggcatattt acccgctgca ttagcgaatt tagacggggc
    25621 attggggcat tttggtaatt tgaccggctc atcggcgacc ttgggcggtt tgcaggagaa
    25681 actaccggca gcagtggtct ttagcgagga aatcggcgag atttatcaga gtttgcaaac
    25741 aatgcgaagc agtttgggta atgcctcggc aaatagctgg gataattggt ttacaccggc
    25801 agagagtgca ttgacagagg tcacagacag ctttgaccat ctagcaaaac cggtggcaaa
    25861 aatgaccgct tggattgtgc tgcgtaccga tgaggaggca gaaaatgaca cagttgctta
    25921 aacatatcgt caaacagggt gagcgttggg ataacttgag ctatcaatac tacggcaatg
    25981 cgtttgaata tggtcgaatt attgatgcca atccgcatat cggtttttgc gaggtgttgc
    26041 cgataggggt aacaatttat atccctgtac ttaacgttaa gccaacgaat aatgaaaata
    26101 tgccaccgtg gctaaggggg cgtgatgcgt aatgtaccga agcccgattt ttcactgttt
    26161 tatgaaaaaa ccaatatcac tgccgagatt gagccacatt tggtgcaact gacctatatc
    26221 gaccatttgg aaggtcaatc ggacgaactc actgtggaat ttgaagacat tcaaggcaaa
    26281 tggatacgcc aatggttccc gactcagggg gataagctca aagcggcgat tggctatcag
    26341 ggcgaatttc tgacggacat cggtgagttt gaaattgatg aagtggaata tagttataag
    26401 ccttccacta ttaacttaaa agctctaagc accggtatca gcaaagccaa ccgtacttta
    26461 aaaccgaaag cctatgagaa tacgactctt gctcaggtgg tggcaaaggt agcagataac
    26521 ctgaaattaa aggtagtcgg taagattaag gctatcccga ttaaacggat cacccaatat
    26581 caagagcggg acgtggagtt tttggcacgc cttgcccgag agtatcatca cagctttaaa
    26641 atcgtgggca atcagctggt ctttaccgat aaaagcgaac tggggcaaag tgagccggtg
    26701 ttaatgttgg aagaacggga tactatttcc ctacgtttgc gagatcggat taaagacact
    26761 gcaaaggcag tggaaatcag cggctatgat acaagcggta aaaaagtggt gaaaaaacgc
    26821 aaaaaggcaa aagccttacg cccgaatata aaacaagcta aatcagccag tgaagatacg
    26881 ctgaaaatca tcacacgagg cgaaagtcaa gaacagattg atgcacgggg tgaggctgcg
    26941 ttggctacgc aaaatgaaga tcaggcggct ggtgatattg agcttatcgg caatcctaaa
    27001 ttggttgccg gagcaacgat tttattgcgt aactttggcg tgtttagtgg caagtatctg
    27061 attaaatcat cacgccatac gattagccgt agcggtggtt atactactca tattgaggtc
    27121 agaatgttgg agtttattgc cgatgattta atcacattag gtatggaggc tggcaatgca
    27181 aacgcataat tttggggcga cttatcaaga agggattgta tccgctgttg atccgaaaac
    27241 gcacaaagtg cggtgtaaaa tccctgcgct ggaagattta gaaactgctt ggctgtcgtt
    27301 tttgaccccg aacgccggtg gcaatcagtt ttactgtttg cccgatgtgg gcgagctggt
    27361 ggcaatgcta cttgatgcac aaggcgaagg tggctgtgtg tttggggcaa tttataacga
    27421 gcaagacccg acaccggttg ccgatagcga tatgtgggtt aagaaattca gcaatggcac
    27481 ggagatttcg cataatcgca aaaccggcga tgtggtagtg aagactaacg ggcatttaac
    27541 cgcaacggct caaggtgggg ctacgattaa tgcaaacacc gtagtcaatg gcacattgca
    27601 cgctaccggt aaaatcacct ctgacgaaga agtctctgct cctgttgtga aacaaggcac
    27661 ggtagagctt ggcacacata aacacggcgg tagccctgaa cctaataagt aattctttaa
    27721 accagtttaa aatccctttt aaagatagcc ctgtatcatc agggctatga acagacaaac
    27781 ttccattcaa ttaacccact ggcaacttgc accctcaacc gatacagttg aggtggtgca
    27841 aggtattgat gatattcatc tttgtattgt caatatcctt tcaacccaaa aaggcacgga
    27901 tattttacgc cctgaatttg gctctgacca ctttcgctac attgactacc ctgaagatgt
    27961 ggctatcccg aattttgtac gggaaattac aaccgccttg caaaaatggg aaaaacgcat
    28021 tgaggttgaa gaagtattgg tggacggttc agctccgcat tttattttta ctgtgatgtg
    28081 gcacttaacc gatgatgttt atcgtgaaat ttaccgaaca acggtataag gataggcaat
    28141 gacagaagcg attaaaatca ttgatgacga tgtaaaaacc gtacttgccg aagcgattgc
    28201 cgattatgaa aaacgcaccg gtaaaatctt acaacctgca catattgagc gttccattat
    28261 tcaaacctac gcttaccgtg agcagttatt acgcaaaggc attaatcacg cctttttaca
    28321 aacctttccg cagtttgcga cggggattgc gttggatttg tgcggggaaa cttttggttg
    28381 ttatcggctt agagataaac ctgcccgtac tttactacgc ttttcagtaa gtggtgaaca
    28441 tacagctata gttatcccta aaggaacgca ggtggcaatt aatgacgagc tatacttttc
    28501 aacgctcaat gacgatgtta ttactccatt aattacctat gtggaaattg atgcagagtg
    28561 taatcaggtt ggtgttatcg gtaatggttg ggaatcagga agagtcagta aactaaaaac
    28621 agcactggca actaattata aagtaacagt tgaaaacatt gatattacga gcggtgggtt
    28681 gatgcaggaa gaagatgaac cttaccgtaa gcgaatttta gccgcaccgg aagcctttag
    28741 taactgtggc tctatcgctg catatgatta ccacgtccgc tctgtatccc aagatattgc
    28801 agatgtgaat atctctacgc ctaggggtgg cttggtaaga attgcggtat tgacccaaaa
    28861 agggttaccg gatacccgat tacttgatga tattaaaaaa cacgttagtg ccgaacggct
    28921 acgcccactt tgcgatatgg tcgaggttat taagcccaca gaacgccgtt atcaaattac
    28981 ggcacgttta atattgttag atggctaccg tgaggatttg gtaaaagcaa aagcccgaga
    29041 ggcattacaa ctctatttgt cagacaagac taaaaaactc ggtttagaca ttgtgccgtc
    29101 ggccatcatt tcagctttaa gagttgaggg tgtttacgat gtgaatttaa ttcagccagt
    29161 caaaactatt atcaacgaaa cagaatgggc gaactgcaca gcaatcagcg tagaagtcga
    29221 agaggagcgt agagatggct agcttgcact atgcggacat tattgtgcga gaccctaaat
    29281 ataaagcatt agcggattta agcaagcggt taaatttgct cgatacttcg caaattatga
    29341 ctacgctggt ggatttattg ggcgacgaat gtatcccatt gctggcagaa aaatggtcgg
    29401 taaccggcta tgacggctta ttggttgcag atagcgaaaa ctcaaagcga gccttaatta
    29461 aaagagcaat cgaactacat caactaaaag gcacgccttg gtcaattcgt gaagtattac
    29521 gcagactagg tttcggagag gttgagattg acgagggact gaaaaatcgg ctgtatgaaa
    29581 gcacttatgt gagcggaatc cccgaaaacg aacgctgggc gcactatgcg attaggttga
    29641 gtaacccgat taccaatgag caagctcgca atatccgtaa ggtcttacgc aattttgctc
    29701 cggctcgttg ctctcttgca gttttggatt ataaagcaga tccaattcga tataacaata
    29761 aagcaaccta caacggcaca tataaccacg gttcaagtta aggataaatt atggcaggtg
    29821 tacaagaaac cgcacagtgg gaaaaccacg tttacagaat tgaagaaaat gacccagtcc
    29881 acggaggaga aaacggtgta accaataagc caattaagca cctagcaaac cgcacgctct
    29941 atctgcgtag attgctcacc gaggcaggac aacgcattaa cccgaaaaaa atcacagcta
    30001 ccagcaaaaa tagtaatgat ttaactgggc atactcacga gattgaccac gcaagcctaa
    30061 cgcaaaaagg cattgtgcag ttaaatgcga gtatccaatc aactagcaca actgagtctg
    30121 caaccccttc agcagttaaa gcagcctatg ataaagcagc aacagcagaa caaaaagcga
    30181 cattggcaaa tgagaaagtc gaggcgatta aagtaggcgg acgaaacctc ctcattcaat
    30241 ccaacactcc acacaacggg aatgcgtact cagtcagata cgcattggct gaggctccga
    30301 aagtaggcga agagattgta gtcactctat ggggtgaact cggctctcaa cgtactggaa
    30361 ttggcgttta caactctcaa ggtgctacgg agttggcaaa actggtcaaa attcgtgatg
    30421 gagtttatca aggtaaaggc atttggcgta agccgatgag tgagggacga gagcgaacgc
    30481 ctaacgacac gcacctgaat gtgtattttt acccgaaaac cgcaacagat aataaccgta
    30541 ttgagcgaat ccagcttgag cgaggctcaa tcggtacaga ttggacgcca aacgaggcag
    30601 acttaaaggc agagattagt gcaatccgca ctaaactaat ccctgtacgg ggtggcaatg
    30661 ttgctacaac cttaaatgcg gctaagcggt cggattggtt tagagaaatg caaatccctt
    30721 atcaaagtgg ttgggggtat tacacccaag catttcataa ctcagggacg caacacaact
    30781 acagcaattt accgctgaca ggtcgttatt attttatcgc cgaactctat cataactctg
    30841 gttattctca cttgcgtatc acctatccaa gtttaaaacg cacctttgag agtgctatca
    30901 accttaacaa tgaggatttg attctagatt ggcgtgaggt ggtgttttta aaagatggat
    30961 tgtatcaagg cacttttaaa acaacaggca cactagagtc tgcttatcgt ctaagagcgg
    31021 ctcgtgatga tgaacgttat cagccatacc tgcaaatggt cgatatgggc tatgaccttg
    31081 ccaatccccc ggtaggaaaa aacaaaatta ttggcagtgt tgattattac gttaaaaacg
    31141 gcacaagcga gtcttccaaa gcgacgatga tggcagcggc aatgcctgac aagaacgtat
    31201 tttgggaagt agcattgtgg aatgcagcac agcaacggaa tgtattgttt aaggggtata
    31261 gcaagactaa caatttgagt attggtaaaa ccaccgacaa tgagcgagac cgagtacaag
    31321 tcaatggcac aatccaagcc accgcaccgg ctgataacgc caataacgac caagtgccga
    31381 ctacggcttg ggtgcgtaag atggcaaaga ttttagctga tggcaaggtg agtaaagccg
    31441 gtgacacaat gactgggcat ttattcatca agaatgggga atactctgcg gtacatacct
    31501 ataacacaag tggttggttc gcaaaatggg aggcggcccc attatctgat tcgcactttg
    31561 ctgccattgt atatgccaac gacagagaga cagttatcaa tcgggtgtta attcccaaga
    31621 agaatggcac agtcgcttta attaatgata ttgccgatac cgttagagcc gctgtgagtc
    31681 gtagtgataa atcccgtagt agaacttttc ctctgcaaac ggggaaaaaa tatacaacca
    31741 cagggagcgt tacgatttat ccagatggcc gaattgtgca gattttccac ttaaaaaata
    31801 ttaaagcggc gtggtttgat tatgaggcaa gtgcggtagg agataggcat agagttgtca
    31861 atatccctct ttggactgcg atgccaaata aaatttttga tgtcgatgtt aaaactgttc
    31921 gcccctcaaa taatgcctca acttactaca tagaagcggc tgaatggctg agtgcgtggc
    31981 agatttacgg taatgacacg aacaaatcaa gtgtcaatat taatctatct cgattcagag
    32041 gtggaaagga tgaagatatg gacttgtatg taacagttga gggatattaa atggcatatt
    32101 ttgtgaatgt gattgatgag caaggcaatt atgagctaat tgatgatgag tttattgggc
    32161 tgtatccgga tttggatttt aacacattac ccaaactcac ggataagcaa tatcagacct
    32221 accttgccaa agcaaacggc aaagagcgat ttgtaaatgg cgaatttgtg tatgagcaaa
    32281 ttaaggtgga tatgcaagcg gtcatttccg ctgaaaaatc tgcaaagtta gcagagatta
    32341 accaaaaagc acaagccttt atcaatgatt tagccaaata caacgaaacg cctaagtttg
    32401 agcgtgatac ttggcttgag caagcgaaag aggcaaaagc gtgggtggct gacccaaccg
    32461 tacaaacgcc aacccttgag ttaattgcgc aaatgcgagg cataccgctc gacacgctac
    32521 gccaaaaagc ctacgaaaaa gcgatggcgt atcaaacggt ggctgctatt gtagcagggc
    32581 aacggcaagg atacgaagac cgcctagaac aagcggaaac cttagagcaa atacaggcga
    32641 ttgagccgat gtatcaatta ccgcaaggag caaacaatga gcaaactgac taaattaaaa
    32701 tcgtggagct accacgtctt aattgcaata gatcaactct ttaacgctct caccggtgga
    32761 gcagcggacg aaacattttc tagccgttgc taccgtggtg cagtgcttgc ggaaaatccg
    32821 aaaaaacgtt ggcgtttttg gcactctttt ttgaattgct tatttttaga cccaaagcac
    32881 tgctacgccg cttataaagc ggaggtacac cgcaagcagt atccgaaggc atttagactt
    32941 ggggggattt aatgaaaaag caaaacttaa aattagtgcg gggcgatgat gtgagtttta
    33001 gattcagcat taaagacagc accggacaac ctcttgcgtt aaaggatttg tggtttgatt
    33061 tgcacttagt cagctatcaa ggtgaggtgc tgattgctct ctctactgat gatggcagta
    33121 ttgagacagc agaggctggt cagatggtgt tacattttag tcaccagcaa actgctcgag
    33181 ctgaatggac aagtgccaat tatgacttgc aactcacgca cgactataca agagtctagg
    33241 aggtggagat gacctctatc attgaactag acctgactga tgacctcatc attgaggtgc
    33301 agttagatag cacaatcatt accggcgata gctttttagc caacctgccg gatttactcc
    33361 aaatttacaa ccaagcaaag tcagatagca gtaaggagct taacaatgac taaacacaaa
    33421 aaccacgaaa accacccgat tgctataaca ttctaccaac ttggcaaaga tgtcggcgac
    33481 attaaccgcc ggctagcggt tattgagcaa cgtgagcaat cagcacagcc aagccaacca
    33541 caagctgagg cggttacaat gccacagcaa gatgctgttg ccattaataa catcaatgcc
    33601 gttttggagg cggtcgaaaa atacaaaact tttgaaaaat acaacactca gtggcaagag
    33661 cgttttgaaa aattcctgtc tcgtaacccg aatgattacg agttaagtga agagcaacgc
    33721 caaaaacttt accacacgat gtgccagcat ttgtggcagg tgaactttac tgaaatcaat
    33781 agtaaacagt atgaattacc gatcccgcaa caattccagg gtaagtttgg ctatattgtc
    33841 aaaacctcgg gcggtagcac agaaattagt tttgagttgc tatctgaaac tcacagcact
    33901 tatctttatc cggatagcta tattcccgag ggcggtggcg atgtaatgct caagccggct
    33961 tatatttatt tagtgattcc ggacttagtg gcaatgcgtg agccggaggt ggtgggggtt
    34021 tcaccgctga aactgaacat tctgcagaaa cacctgctga cacctcagaa actacaggag
    34081 cagaagccct tgaacacgaa ggcaaactga ttgatggcgt atggcatttt gagcgagagc
    34141 cggtaaatcc accgagagat cagaattatt atgtggcagg taacctgttc tacaacggca
    34201 taccggattg ataaattgat gtttcagtaa acctaattag gggggaatcc ccctttttta
    34261 ttattataag gaaagaaaat gaataaatct ttagtagcgt tagctgttgc agcgatggca
    34321 tcgaaagcac aaactaaaca gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa
    34381 gcaagcaagc aagcaagcaa gcaagcaagc aagcattgtg agatagagaa agtatttaaa
    34441 caagctcctt tacccttttc agggcaaaaa cggatgtttt tatcccattt caaacaggtg
    34501 ttaaatgcaa atattgaagc agacgggaaa gattggacta ttatagatgt ttttggtggg
    34561 agtgggttgc ttgcacatac cgcaaagcgt gagaaaccgt tagcaagagt gatttataat
    34621 gattttgata attacgccga acggctaaac catatcaaag agacaaatca acttcgccaa
    34681 gaaatctacc aaattgttga tgaaattata ccaaaaaata agagaattag caatgaaatt
    34741 aaagctaaaa tcatcaataa aatcaatgat tttgaggggt ttaaagacct caaatgctta
    34801 agttcttggt tattgtttag cggtgagcaa gttgcaactt tagatgagtt atttaagcac
    34861 gatttttggc actgtgtgcg gcaaagtgac tatcctgaag caacgggtta tcttgatgat
    34921 attgaggtgg ttagtgagtc ctttcatcaa ttattgccga gatttcacga caaagagaaa
    34981 gtattattga ttcttgaccc accttatctt tgcacaagac aggaaagcta taagcaggaa
    35041 aattactttg atttaattga tttcttacgg ttgatagacc ttactaaacc accttatatc
    35101 ttctttagct caaccaaaag cgagtttatt cgctttattc actatatggt tcaaaacaga
    35161 aaagaaaact ggcaagcgtt tgagggagcg gaaagaatag tggttaatgc ctcggcaagt
    35221 tataacggca aatatgagga taatatggtg tataagttct aaaaatttaa acgccctttg
    35281 atagtgattt aaagggcgtt aaaaaaccat taaaattaag aaagtaaagc tcctgttttc
    35341 tttcgtagtt ttcgtggttg cgtttcgcag ttttgttggc tcgctacaat tactgttcat
    35401 tgtaatgttt aatcgaccat tggttttgct aggatcagtt tcaccaaaat aaccgttaaa
    35461 accttggacg gtgccttctg ctgctttgat tttttcagca agtatttgtt gctttgcagc
    35521 actttcatct gttaccttga gtacttcaaa gtattcgttt tcacgaatga gaccagtagc
    35581 aaagtctaaa tcgatgaaaa ctttacgcgc attaaaaata gctgtgatct ctgcaacgct
    35641 taaaggtttt ttcaaacgga taaaattagt tggcaagtgt ggaaaacgtt gatatgtgaa
    35701 aatatctttg tccaaatgac gagaaacctg atctggacta gtaattgctt gaactttcac
    35761 ttggtcaatt gttggactaa ctatgccggt tagtgtaatg tttgctgttt gggctgtatt
    35821 atgattgaca accatcgctc cttcatcgct attctgaata cggcggaacg tgagatcatt
    35881 gccgttgaca tctaagcgcc caccacgata accaaaatag atattgttag gatccatttg
    35941 atctgatgaa ttgaggatta cggtaggtct gccactcaca atacctagct ctgaaaatgc
    36001 agattttttg ttattttcat ccgcttgttg atttaatata acagtgccgt caccaacgct
    36061 aacggagcct ttgttttctc ctttgccgtt gatagttagt gtcccttgtc cgatttttga
    36121 taatctgtca ccagcagggt tactgatttt ccagatcaca tttttatctt tatcaatatc
    36181 aacaccggct cctaaccaag taatgcctgc tttcatacct tccacagtgg tattaccatg
    36241 aaaatggatt gctcctgcac cttggttgat attctctgtg agacttagcg tattgttttc
    36301 acccaaaata tggaatgttt taccgtgttg gagttgagga ttccaagggt tatcgcctgt
    36361 attaccatta tgaccttgaa gtgttggtaa tgaagtatct gctaaattaa ctgtaagagg
    36421 agcattccct cctacgattg agctggtatt gccagaaggt gtccaagtga gtctagcacc
    36481 atttgcatta atagcgcctg cattattttc tttttctaaa gcacgtacat aatgtgggtg
    36541 gtgatagttc cagtaagatt tataaacatc tcccccaagc gtattattct ctgagaaata
    36601 gtcactatat acacctaaca caacccattt tttggtacga acatcataac caaaaagagc
    36661 ggagccacta tcgccaggta aaccgagagt tgttaaggga ccgtagccgt ggtctttaaa
    36721 tacatcgttt atggtattat tttcagtttt tgctactaaa atacggctgt taaaatcgct
    36781 gcctggaacg cttggatcgc cattagtaac aggcagaacg ctacctcctg tcaaaaaagg
    36841 accgttaccg acttcaatac gcccgttgtt taagcgatct ctttcgttgt taaccactgc
    36901 ttgtctacca gaaccggcac gaataaacat ggggaaaata aaagggtcgc taaatacact
    36961 ataaaaatct tgtacatttt cggtagtgcg ttcaatctca ttagctggag cgacttcagt
    37021 tactaatttc gataacctag gtgcgtggta gtcccagcgg ggcgttgggt tacctgaacc
    37081 atctaaaatc agtttttctc cttcgtcatt acgttcacgt ttttctatgc ctttcccgag
    37141 atctgttctt tctttatttg gatcaggcat atagttatta cggcgcacca cactataatt
    37201 gtaatgatgg tcgtcggctt tttcgctaga tgaatcacca aattgaatgt cccgatagcc
    37261 ttggttgtgg tttacactaa taacgtattg tggattaact aatgttgcaa tccctgtttg
    37321 acgagtaatg gaagagaagt caatcattgg aatagacata gtctcacctg ttactgtttc
    37381 aaaaacaggt agatcattag agtcttcatc acctccacca tagagaaatt cataacaacc
    37441 gggtaaactt tcattatcaa agcattcaga taggcttaca ttcggcttag gtgaaatatt
    37501 tgattccact tcaacctgct tagttggtgt aacctgtaaa aaagtaccga tgtttttgcc
    37561 cgttttatca ataactggaa tattggttgc cccaacaaaa aatttgcctt tattttctgc
    37621 taaatcacga aaatattgat aatcgacatc accacgtaca atagatgcca gtaatgttgg
    37681 tgaaagagaa atagcaattg atgttgctag aagagagagc ttgaaagtat gcttcattat
    37741 tatttgaaac ctgtataaat atgtaagtag atcagatttg agcgctatta aaaagattat
    37801 ttaaatcgat taaatattca tttaaatacc acacaattat tcagcgcgat tatcttgttc
    37861 ggcataaatc tg
//
LOCUS       DANLXS010000005        36828 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.005, whole genome shotgun sequence.
ACCESSION   DANLXS010000005 DANLXS010000000
VERSION     DANLXS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 36828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36828
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.005"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(199..1074)
                     /gene="ubiA"
                     /locus_tag="PXD71_000208"
     CDS             complement(199..1074)
                     /gene="ubiA"
                     /locus_tag="PXD71_000208"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDL6290377.1"
                     /translation="MNNETLFSKQKWIAYAQLMRFDKPIGTLLLLHPTLWALFIAAEG
                     MPPVAILAIFTLGVIVMRAAGCVINDYADREIDGHVKRTSQRPLATGRVTTTEAKVIF
                     AILLLFAFLLDLMLNRYAFLLSFVAVFLAVIYPFMKRFTHLPQVVLGMAFGWSIPMAF
                     GAVTETLPLECWLLFFANLAWTVAYDTQYAMVDRDDDLRIGVKSTAILFAQYDNKIIA
                     LLQFITLILLGLLGVVKDYHIGYFVVLALSATLFIYQCWLTKKRERSECFKAFLNNHY
                     FGLGVFVAILVGIYF"
     gene            complement(1067..1828)
                     /locus_tag="PXD71_000209"
     CDS             complement(1067..1828)
                     /locus_tag="PXD71_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDL6290378.1"
                     /translation="MKQANRHKKIIELVNQLGYVSTEQLVAELNVSPQTIRRDLNEMA
                     ENNLIRRHHGGAAAPSNTENSDYTHRKQFFSHEKNAIAQQVAKLIPNGASLFLDIGTT
                     SEAVALALLSHKNLKVVTNNLNAAHILMQNEDCQITVAGGGLRSDGGLIGEETVRFIN
                     QFRLDFGILGISAVDMDGSMLDYDYHEVQVKRALMECSRQVVLVTDHSKFTRTAIARL
                     GNVKEVNYLFTDQDLPLELQTHLSQSDVIVKICNE"
     gene            complement(1895..3262)
                     /gene="purB"
                     /locus_tag="PXD71_000210"
     CDS             complement(1895..3262)
                     /gene="purB"
                     /locus_tag="PXD71_000210"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL6290379.1"
                     /translation="MELTALTALSPIDGRYQDKVASLRPIFSEFGLLKFRVTVEVRWL
                     QKLASHTQINEVPVFSEKANDYLNQIVADFSLEDANRIKTIERTTNHDVKAVEYFLKE
                     KCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKVIDAVAELAKC
                     YQHIPLLSRTHGQPASPTTMGKEMANVAYRLQRQYKQLENLEILAKINGAVGNYNAHL
                     SAYPEIDWHTFSQEFVESLGVTWNPYTTQIEPHDYIAEFFDCVARFNTILIDFDRDMW
                     GYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAVMAHLGQKLPISRWQ
                     RDLTDSTVLRNLGVGLGYALIAYASTLKGISKLEVNEQHLRDELNQNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDETAMREFIDKLEIPENEKNRLKEMTPASYIGYAVE
                     LVDKL"
     gene            complement(3296..3925)
                     /gene="hflD"
                     /locus_tag="PXD71_000211"
     CDS             complement(3296..3925)
                     /gene="hflD"
                     /locus_tag="PXD71_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDL6290380.1"
                     /translation="MADYHDITLAYAGVCQAATLVQQFAHKGMADREAFKCALESLLI
                     TQPESTLSVFGDDLSHLKMGLETALAQTGGGNGKLDTEVGRYWISLLALSQKLNKNPE
                     AKQQLAQRLQQVERQLALYEDNVMADQMIANLAAIYSDIISPLGTKIHVMGMQDYLVR
                     PDIQNKIRASLLAGIRAGILWQQVGGSRWQFLFSRKKIFNQAQKFYRQI"
     gene            complement(4057..5721)
                     /gene="cydC"
                     /locus_tag="PXD71_000212"
     CDS             complement(4057..5721)
                     /gene="cydC"
                     /locus_tag="PXD71_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDL6290381.1"
                     /translation="MKSLFPFFALYQTHFGRLLLGVVLAILGLAASIGLLSLSGWFLA
                     ASFLAGNALIFNFFYPSSGVRGLAVGRTISRYFERLVTHDATFRVLANLRASVFKKLI
                     PLSPSGLNRFRNSELLNRLVADVDTLDTLYLNLVSPFVSAVMIIAFMAIGLSFVSVPL
                     MLIICGTLLVLLAVIPTVFYRLGLKLGRNAIQSRANYRSQFIEWVQLNAEFLLFGNLN
                     QMSEKLQQTEQQWLNAQSKESRLSGLSNSLIMLSNGILTCVVIYLVSTSINVPTAKYP
                     EALIALVIFCVMASAEILSPIGIAFLHLGQVITAAERISEITEQQPNVTFGSKAEWQN
                     LSQNQPLVRCENVSFAYYGEQNVLNNLSFKVLKGQKVAILGKTGSGKSTIFQLLNRNY
                     DPTSGQIWLNNCKIDEYSEPMLRSKLVTLSQRVHIFSQTLKDNLLMGNAQATDEQMQE
                     AVRKVGLGHLLETDGLNLWLGEGGRPLSGGEQRRLGLARLLLSSAELVLLDEPTEGLD
                     RETEQQILNLILTHCCDRTLIMITHRQSGLDKFDVVYRIDNGRLVG"
     gene            complement(5721..7487)
                     /gene="cydD"
                     /locus_tag="PXD71_000213"
     CDS             complement(5721..7487)
                     /gene="cydD"
                     /locus_tag="PXD71_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDL6290382.1"
                     /translation="MEKQRQRELQKWLKSQQKVIKKYMHLNILLGSFSSLCMIGQMWL
                     IATMLDKMLIGKQSPNSFLVEIMLLFCCFAARAFFIFLRERVGFKAGQTLRLHLRRQI
                     LAKMEAVGPMSIQQKPAGSWATLMLEQVENLHNFYARYLPQQFLSLIVPLLILCFVFP
                     INWAAGAILFATMPLLPLFMILAGMKAVEANQRNIGILSRISGQFLDKLKGLETIRLF
                     GQAEKQTEQIYQSTEDFRISTMDVLKMAFLSSAVLEFFTAVSIAVTAVYFGFIFLGEL
                     DFGYYGTGVTLFIGFFCLMVAPEFYQPMRELGVFYHDKAAAISAADNIETFLKEKVKT
                     QGGNLQENVENQPLVIQAKDCVIFSPQGKALTQALNFELNAHQHIALVGQSGAGKSSL
                     MNMLLGFLPYEGSVTINGTELRDLNLSQWRAKLAWVGQNPQLMHGSLKENILIGNPNA
                     TDSELAKALQLSKADEFVARLGLEHQVQDSNIGISGGQAQRIAIARALLRPYELLLLD
                     EPTASLDMDSEQQVLAALHNLSREQTTLMITHRVEDLTQCDEIWVMKQGQIIQKGRFT
                     ELEHTGFFAELIHNELVHQGEI"
     gene            complement(7602..11699)
                     /locus_tag="PXD71_000214"
     CDS             complement(7602..11699)
                     /locus_tag="PXD71_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HDL6290383.1"
                     /translation="MTDENKQTATNQTDSAQEPQATQPKKSKWRFLKHGFCLTFILLL
                     LAILFLCTGYGQRKVLSWVDKFVEPLQLGQVQGSLQDGLQLSDARFVMDGVNVTVGQA
                     DLHIGFSCLLHYEMCLNNLSLKDTEVLIDTTKLPPSQPKEETEPFTELNLPLGISAQN
                     INLENIKVKVDDMDIHLNHFHTALSGKGRAITVHPTQLEGLNLLLALKLDETDTEQAV
                     DFAQKNAKTEALSATNPQEIADKVIQEAVEKTSEKVVEGFHQLQRKTVAQAEQVDNKV
                     DWAAIKAQLAQPLLDKNIRFSLPLDLNIEQIDIANVAIAQKAKDEKGNLIEPISLLNV
                     ASLQLQAQAKDQHINLKSLDFKSDQGDLTASGSLSLQDNYPLSWTLSGHEAKTAKVKL
                     PFNQIEASLSGELYDKTSLKVKTAGAISASLEGNVALAQAKTPFDLSLKSESMRYPFA
                     PQQNGDVLSLENIAMRLNGNLLDYALEAKVSAKGMGVPASSASLTGKGELTHFNIQDL
                     SLNTLEGTAKIDGLVDWSEGVAWRSNLKLQHINTKSLLADWPAVLSGSLSSEGYAGRG
                     NSASGWKADVSNIDIKGSLLQKNLHLSGNLKADHQQLLDVPGLSLVYGENKIDLKGVL
                     GEKSDFYADIKAPNLQGLVPNLKASVNGNAKLSGKVSEPNIDLDLVASNVGYEQFKLQ
                     NLTAKGKITTEKTIQGDLTLGLRQFAYNEIKVGNATLLLKGNEENHSLKLSAKGNPVG
                     ADLQISGKFDRLKEIWEGQLSQVSIQSTEFGQFQADKAVNVKYDNKAINANISAHCWH
                     NRKINLCFPTAFNAGVEGKVPFEIRNFDLAVLQQFLEKNSQLSGIVNAKGDAAWFKNK
                     QPQVNLDLTSNAIKFIQKMEGGKSFPLTVSPLKIDLKMSDNNLTLKSDLRVENNGRLS
                     TDLVMKDLSKARALSGNIHLDQLSLKLIRPLLERGDSVDGNVNARLTVSGTATSPLLH
                     GNLNVTELKARSVTMPFDITGGNLAMNFHGATSTLSGKLQTKQSELRLEGDADWRDLN
                     AWKTRVHAQANRFQVDIPNIAKVAVSPDIQVTATPTLLTLSGNVDIPWARIEVEQLPE
                     SAVSVSGDEVIMDGSVKNKVPFSQRNIPAQTSGGMAINADIKINIGNDVKLKAYGLNS
                     NVNGLLSVRQGKQGLGLYGQVHLREGRFAAYGQDLLIRKGDISFAGSPSQPTLDIEAI
                     RNPEAMEDPTITAGVRVTGLADNPTVKVFSDPAMSQNEALSYILTGRSLESSGDTSSS
                     NAVAAALLSMSLSKSSKLVGDVGSTFGLKDLSVTTAGIGDNTKVEVSASLAPKFRVKY
                     GVGIFAPLTELTLRYNLTPRLYLQWVSSINQAVDLMYRFEFDELF"
     gene            complement(11780..13615)
                     /locus_tag="PXD71_000215"
     CDS             complement(11780..13615)
                     /locus_tag="PXD71_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HDL6290384.1"
                     /translation="MNTKLHFLTFCCLVAFASMAQANQEQRLSDVDRERVENLQEKLA
                     EDSDDEPEIEPLVTLEVKGIQGKDAKKNVELYLDQIANEYADGSERYQYLVQTTVDKA
                     LRALGYYNSRYQFVQLPQEGKKPLLVLNVELDKETVKIDETDIVLQGDAKTDEDFAHL
                     VSKAPKQGSRLNHKTYDNFKGDIESTAFSKGYFDGHWLYHRLEVYPTDHKADWRLGYD
                     SGKRYHYGQIRFVDSQIKEEYLQNILKIHSGEPYLANDLSILTQDYLSSNWFSSVMVE
                     SHLNEQDKLVDLNVLFQPKKRNEVEIGIGYETEVGPRLQLNWKKPWLNSRGHSIESRT
                     YVSKPEQTFEFGYNIPLREDPLHYYYQLSGTLENEDQNDTKSTAATLGLQRFWTHETG
                     WSFSVGLKARYDSFTQANDRFKTLLVYPTASLNRTRTDGKPYPLWGDTQKLTVNWGSR
                     AVGSDVNFYSYKASTSWARTYFDNHRFYLRAELGYLKANQFERIPPALRYFAGGDMSV
                     RGFGYKDISPRDPSNGKRVGGSHLATGTVEYQYQVYPNWWGAIFYDAGLASNKFKTQD
                     LYYGTGLGVRWASPIGAIKFDVATPVKSPDNSKGIQIYIGLGSDL"
     gene            complement(13627..14472)
                     /locus_tag="PXD71_000216"
     CDS             complement(13627..14472)
                     /locus_tag="PXD71_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDL6290385.1"
                     /translation="MFKQINKRLNFNIFLQMFVLFTPLVQAAQMAIVIDDVGYRIKED
                     REILALPKAVSVAIIPVAPYATERAKDAYNQKRDILIHLPMEPKSKQPIEEGGIHIGD
                     NEEKIRKLIHTSRGQVPYAIGLNNHMGSGATSDSATMQHLLKVLKENTLFFLDSKTIG
                     SSVAAKTARQFGINTLERDIFLDDSDLLADVQKQFAHAINHARKNGVAVVIGHPRKNT
                     ISVLKQNLAQLPQDIELVSVGNLWRNEKTVPDKPFIMLFEQEPALSSVPPYDSIPLLR
                     GIPKE"
     gene            complement(14473..15636)
                     /locus_tag="PXD71_000217"
     CDS             complement(14473..15636)
                     /locus_tag="PXD71_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL6290386.1"
                     /translation="MLVKRINSYCWTAIFSGVISFSTPFSFANNLNQIQQKIQQQTSK
                     INEQKQKRNALQSTLKNQEVEMGKVLDKLQKTEMSLTEVRQTIKATETEIARLEKLEK
                     QQKEKLKEQLDSAYRSGVNPSVLERLMSEEAKNAERMTAYYQHMNNIRIDAIVEIRKT
                     QADLKARRDELKGQQKDHQTQLNEQKKQEKDLKKVQSQRESTLRLIDKTLEQDQSRLE
                     NLRSNEQALKQQLEQAQREAQQAEKREQQREKQAKSGQSKTTENVERTPIKAGRYTMP
                     VSGNIITPFGNNWHGVVIGASAGTSVRAMASGRVIMAQWLAGYGNMVAIDHGNGDISL
                     YGYNQSISVSKGSRVQGGQVIAKVGNSGGQSRSALYFGVTRKGTPINPLNLVK"
     gene            15804..16487
                     /locus_tag="PXD71_000218"
     CDS             15804..16487
                     /locus_tag="PXD71_000218"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL6290387.1"
                     /translation="MQLVFIRHGFSEWNAKNLFTGWRDVNLTERGIEEAKSAGQKLKA
                     AGFEFDIAFTSVLTRAIKTCNIVLEESDQLWIPQVKNWRLNERHYGALQGLDKKETAE
                     KYGDEQVHIWRRSYDISPPDLDPQDPNSAHNDRRYANLPKDLIPNAENLKITLERVLP
                     FWEDHIAPALISGKRVLVTAHGNSLRALAKHIEGISDADIMDLEIPTGQPLVYELDDN
                     LKVISKRYL"
     gene            complement(16624..17181)
                     /gene="frr"
                     /locus_tag="PXD71_000219"
     CDS             complement(16624..17181)
                     /gene="frr"
                     /locus_tag="PXD71_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL6290388.1"
                     /translation="MINEIKKDTQDRMEKSLEALKGHIAKIRTGRAQPSLLDGIQVEY
                     YGSATPLRQVANVVAEDARTLAVNVFDKSLISAVEKAILTSDLGLNPSSAGTTIRVPL
                     PPLTEERRRDLIKIVKAEGEQGKVAIRNVRRDANDQIKALLKDKEISENEQHKAEEDI
                     QKITDTFIKKVDEVLAAKEKELLDF"
     gene            complement(17233..17949)
                     /gene="pyrH"
                     /locus_tag="PXD71_000220"
     CDS             complement(17233..17949)
                     /gene="pyrH"
                     /locus_tag="PXD71_000220"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL6290389.1"
                     /translation="MSKPIYKRILLKLSGEALQGSEGFGIDPSILDRMALEIKELLAE
                     GVEVGVVLGGGNLFRGAKLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAEVNAKLM
                     SAFQLNGICDTYNWSEAIKMLREKRVVIFSAGTGSPFFTTDSAACLRGIEIEADVVLK
                     ATKVDGVYDKDPAKYADAKLYNKLTYAQVIEQELQVMDLAAFTLARDHGMPIRVFNMG
                     KPGALRNVVFGTEEGTTISE"
     gene            18041..19603
                     /locus_tag="PXD71_000221"
     CDS             18041..19603
                     /locus_tag="PXD71_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL6290390.1"
                     /translation="MKSKQLFGILVAISLATAASCLMLIGSGFFPNPSIFEVILGVFL
                     IIALASSKWTYYLLLLPISIAYAIYTPVGIKFGKPTYEYIASIFATDMMESKEFIAQL
                     PILNIAAGIAIVLAVIFYRKITNRYQIRFLRNKTFVISAFVLMLFSLAPFKFFQEFYN
                     SSLKVKKELEVLNNLTLDSQWGQSTLSSTSNYDDYVLIMGESARKDYHHAYGYPAANT
                     PFMSSAKGTLIDGLTSGGTNTIASLRLMLTKPDTEKWEANYGLTLIDLIKSTGIKTYW
                     ISNQGYLGNYDTPISSIGNKSDVKIFTKAGDSLNTNVSDFELLPHFAKVIEEPAQGKR
                     FIFLHLYGSHPITCDRLTDYPKMFDDDKLPKRYFNVNCYVSSIKKTDELLKRVYDLLV
                     KNQENHQRSFSMVYLSDHGLSHEVSKENIVIHNGKDRSKLHYDIPLFKISSDDTQRNE
                     YKAFKSGLNFTNGIANWIGIENPLLDKEINLFNEQNDKSDYGLKKIIETQSTVEDPAV
                     PIPESYLYKATN"
     gene            complement(19667..20518)
                     /gene="tsf"
                     /locus_tag="PXD71_000222"
     CDS             complement(19667..20518)
                     /gene="tsf"
                     /locus_tag="PXD71_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL6290391.1"
                     /translation="MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKS
                     GQAKAAKKAGRVAAEGVILARVASGFGVLVEMNCETDFVAKDAGFLELANEVADFAVA
                     NKGTTIEALQAQFEEKRAALVAKIGENMNIRRVQYLEGEVIAQYLHGAKIGVLVAGAG
                     SEDELKKVAMHVAASKPEFVNPEDVSADVVAKEREIQIEIAMNSGKPKEIAEKMVEGR
                     MAKFTGEVSLTGQPFVMDPSQSVGQYLKSVNTSVSNFIRLEVGEGIEKVEEDFAAEVA
                     KITGGNA"
     gene            complement(20614..21333)
                     /gene="rpsB"
                     /locus_tag="PXD71_000223"
     CDS             complement(20614..21333)
                     /gene="rpsB"
                     /locus_tag="PXD71_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL6290392.1"
                     /translation="MAQVSMRDMLNAGVHYGHQTRYWNPKMKPFIFGARNGVHVINLE
                     KTLPLFNEALAELTRIASNNGKVLFVGTKRAASDAVKAAAVESQQFYVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQTQDGTFDKITKKEALMRTRELEKLELSLGGIKDMNGLPDA
                     IFVIGADHEHIAIKEANNLGIPVFAIVDTNSTPDGVNYIIPGNDDATRAIQLYLDSAV
                     AAIKEGRGVETVEVAEEASAE"
     gene            21678..22706
                     /locus_tag="PXD71_000224"
     CDS             21678..22706
                     /locus_tag="PXD71_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6290393.1"
                     /translation="MKKTLSALAIAAATFTSTSTLAANEVNVYSYRQPYLIEPMLKNF
                     EKDTGIKVNIIFADKGLVDRVKQEGELSPADVLLTVDISRVMEIVNADLAQKIDSKVL
                     EKNIPAQFRDSNDQWFGLTTRARVIYTSKDRVGKLPAGFDYLDLAKPEYKGKVCVRSG
                     KNSYNVSLFAAMIEHYGIEKTKAFLEGLKANLARKPQGGDRDQVKAIKEGICDYSIGN
                     SYYYGKMLDDEKQKSWAEAAIINFPSGEHGTHKNISGVVIAKHSPNKANAVKLIEYLS
                     GEKAQGLYAELNHEYPVKEGIEPSAIVKGWGTFKSDTIKLEDIAKNYEAALKLVDEVK
                     FDDFSEKK"
     gene            22829..24475
                     /locus_tag="PXD71_000225"
     CDS             22829..24475
                     /locus_tag="PXD71_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6290394.1"
                     /translation="MRSQFCWKSIAALTTFIVLLPLIAICYHALQGDNENLAHLWQTM
                     LPIYLKNSTLLVLGTVFLALIFALPTAWIVANYRFWGQRTLQWLLCLPLAMPAYLIAY
                     LYTDLLDYSGAFQGWLRSIFGWTHPQDYWFPQIRTLYGACFVLALVLYPYIFLLVRVA
                     LLEQSENLTHSAKMLGASPSQLFRRITLPLIRPSVAVGIALVAMETLGDFGTVAYFAV
                     PTLTTAIYDSWLGYHDLGTASQIAIFMLLMIFLFLSLEQYSRRKQKSYQRGYEKKSAF
                     KFLTGWKLALALAWCWGLLALAFFIPFGRLLYWAYDYFEQAWNLDFVQFSLNSLKVSI
                     IASVITVLIALLLHFSKRLNTRKILVKNDRLSLQLASLGYAVPGTVLAIGLLSPLTFA
                     DHQLHAWLKSLELSPVGLIFSGSLFALVLAYTIRFLAMAIGSLETSLNKISPSLDMAS
                     QTLGKNGSAMLVKIHIPLIYKGILTAGLMVFIESMKELNASLLLRPFNFDTLATYVFT
                     FTSDEQLERAALPAIVLVLVGLLPVIWLTRSLISQSEKER"
     gene            24485..25495
                     /locus_tag="PXD71_000226"
     CDS             24485..25495
                     /locus_tag="PXD71_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6290395.1"
                     /translation="MSVLTINHLNLNFGSTKVLQNLHLAVNQNEIICLLGASGCGKTT
                     LLKAIAGLLPIEQGDIHLAGECLKSKAVEERQIGLIFQDYALFPHLTVADNIQFGLSK
                     LPKVQQKAITEKMLSVVHLQGFESRFPHELSGGQQQRVAIARALACQPELLLLDEPFS
                     NIDSQTRYAMIQEIKQILKSQNVPAIFVTHSKEEAFAFADKIAVMDQGKIIQFGKPNE
                     LYHSPVNPFVADFLGGTNYLNCNVTEDNVLCSPIGEFRLFPEMNVQKGRYRWLLRPEQ
                     IILKPNELGLGKVVGKLFLGLYYRYQVEIKGIEIVTYQSQELKIGEQVEVGFQVREFI
                     LF"
     gene            25505..26131
                     /locus_tag="PXD71_000227"
     CDS             25505..26131
                     /locus_tag="PXD71_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HDL6290396.1"
                     /translation="MKIEVWSDYACPFCYIGKRHLEQALAQFEGEVEVVFRAFELDPH
                     ANGEPEGDIQQRLMRKYQKTAEQADEMIRYVEQAGKQAGLDLRYRTTQYTRTFEAHRL
                     AKFAESKDLGEAMVERLFKAYFTDNTILAKRTELISLALDIGLERDEIAQLLTGDDFG
                     HEVREDERVAHKYGIHSVPFFVINEKLGVSGAQPPEILLDAIKQALQK"
     gene            complement(26193..27683)
                     /locus_tag="PXD71_000228"
     CDS             complement(26193..27683)
                     /locus_tag="PXD71_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDL6290397.1"
                     /translation="MSLYQIARTETGKVISLNAKMANRHGLIAGATGTGKTVTLRKLA
                     ESLSDDGVPVFLVDVKGDLSGSVQAGNFSGKIAERILQFNLGGEDYLKGYPVSFWDVF
                     GTSGIPLRITISEMGPMLLSRLLNLNDTQGGLLNLVFRVADDRGLLLIDLKDLRALLK
                     FVADNAKEFQLEYGHISAASVGAIQRALLRLENEGADNLFGEPALDLHDWIQTRNGRG
                     VINMLNAEKLINSPRVYSAFLLWFMAELFENLPEVGDPEKPTFVLFFDEAHLLFDDAP
                     KALVDKIEQVVRLIRSKGVGVYFVTQNPLDIPDSVLGQLGNRIQHALRAFTPRDQKAV
                     KSAAETFRANKEVNVVEAITQLGVGQALVSVLDEKGMPTPVEIAYIYPPKSQLAPLAT
                     GERDSLVKQDDLYHHYSQYVDNESAFELLNAKAEAIKAEQAAAEEANNDFFGGILSSI
                     FGSKKKKDQSITEQVVSQVAHSVGRNLRNQVTKQIMRGILGAITKK"
     gene            complement(27742..28977)
                     /gene="rhlB"
                     /locus_tag="PXD71_000229"
     CDS             complement(27742..28977)
                     /gene="rhlB"
                     /locus_tag="PXD71_000229"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDL6290398.1"
                     /translation="MSNHLTEHRFIDFPIHANVLNALDSKGFEFCTPIQAKTFPITLK
                     GKDIAGQAQTGTGKTLAFLIATFNHLLNNEIETSKNQPRALILAPTRELAVQIGADAE
                     LFLKHSDLKLALAYGGDGYDKQVQEIEKGVDILVGTTGRVIDYVKQGIINLDKIQVIV
                     LDEADRMFDLGFIKDIRYLMRKAPKPSERLTLLFSATLSAKVRELAYEDMDNAEYIEI
                     EPLQKTGHRIKEELFYPSNEDKMALLLTLLEEEWPERCMIFANTKHKCEEIWQYLSAD
                     GHRVGMLTGDIPQKKRLSLLDNFTKGNLDILVVTDVAARGLHIPDVTHVFNYDLPDDK
                     EDYVHRIGRTGRAGESGHSISFACERYAVNLPAIEEYIGHQIPVSQYDKESLLTLPKV
                     NRSRSKKTFLAKTKKNSRN"
     gene            complement(29065..29787)
                     /gene="recO"
                     /locus_tag="PXD71_000230"
     CDS             complement(29065..29787)
                     /gene="recO"
                     /locus_tag="PXD71_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL6290399.1"
                     /translation="MTIDQWQRAFVLHRREYSENSLLVEFFTEHHGRITLLAKGARRA
                     RSPLKAVLQPFTPLLLRWSGKGDLKTLTKAEPAALTLPMETLSLYSGFYINEVLARVL
                     ENQTAYPELFQHYLRCITELATQKQIEPILRTFEFHTLKALGYGVDFTHCAATGLPVD
                     PKMTYQFQENQGFIASLLQNNQSFLGSDLLAFAQLDFSEKSTQQAAKRFTRMALKPYL
                     GSQPLKSRELFQSILPNKLKSG"
     gene            complement(30017..30934)
                     /gene="era"
                     /locus_tag="PXD71_000231"
     CDS             complement(30017..30934)
                     /gene="era"
                     /locus_tag="PXD71_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL6290400.1"
                     /translation="MTEQIQTPAKTYCGFIAIVGRPNVGKSTLLNKILGQKISITSRK
                     AQTTRHRIVGIHTEDQYQAIYVDTPGLHIEEKRAINRLMNRAASSAIGDVDLIIFVVE
                     GTKWTEDDEMVLNKLRSAKAPVLLAINKIDNIKEKEELLPHITELSQKFDFAEILPIS
                     AQRGKNVHILQKFVRQSLRQGEHHFPEEYVTDRSQRFMASEIIREKLMRFTGEELPYS
                     VTVEIEQFKMNERGVYEINGLILVERDGQKKMVIGAGGQKIKTIGIEARKDMERLFDN
                     QVHLELWVKVKAGWADDERALRSLGYMDE"
     gene            complement(31037..31711)
                     /gene="rnc"
                     /locus_tag="PXD71_000232"
     CDS             complement(31037..31711)
                     /gene="rnc"
                     /locus_tag="PXD71_000232"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL6290401.1"
                     /translation="MQLERLQKKLGYQFHNLDYLIQALTHRSAATKNNERLEFLGDSI
                     LNFTIGKVLYDKFPKANEGELSRMRATLVREQTLAIIARKFDLGDYLKLGPGELKSGG
                     FRRESILSDCVEAIIAAIYLDQDMAKAMEKVEDWYSDLLKDISPGDSQKDPKTRLQEF
                     LQGRKLPLPEYDVLDIKGEAHNQTFRVTCKVINVEEVFIGIGTSRRKAEQNAAEKALA
                     VVKTKK"
     gene            complement(31757..32716)
                     /gene="lepB"
                     /locus_tag="PXD71_000233"
     CDS             complement(31757..32716)
                     /gene="lepB"
                     /locus_tag="PXD71_000233"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL6290402.1"
                     /translation="MAQFMPIIFLGIIYGIWQWLDSMQLPNTISIILVALVIICGGFW
                     AFYKFSADPRRKQAIVLQQKRLGRELTAEEIAEIQPKSAVGEFFASLFGILFFVTVLR
                     SFLFEPFQIPSGSMEPTLRVGDFLVVNKFSYGIKDPIWQNTLIETGKPERGDVIVFKA
                     PAQPHVDYIKRVVAVGGDKIKYDFATQQLTVTKANGETKVFEYSEGKPNPEFFYHGEM
                     QVERTEKGNVTHQILNHPMAFNYAPYFYQQEGQAEGEWVVPQGYYFVMGDNRDNSEDS
                     RFWGFVPERNIVGKATFIWLSLDKKANEFPTGLRFSRMFTSIN"
     gene            complement(32803..34596)
                     /gene="lepA"
                     /locus_tag="PXD71_000234"
     CDS             complement(32803..34596)
                     /gene="lepA"
                     /locus_tag="PXD71_000234"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL6290403.1"
                     /translation="MQNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLNYKAKDGEIYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAIEIDLEVVPILNKIDLPAAEPERVAEEIEDIVGIDAIDAVR
                     CSAKTGLGIEDVLEEIVKKMPAPEGDAEAPLQALIIDSWFDNYLGVVSLVRVKNGVLK
                     KGDKIKVMSTGQSYNVDRLGIFTPKQVDTTELKTGEVGWVVCAIKDILGAPVGDTLTH
                     HHNPATEVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLNDASLFYEPETSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVVQTSGETIYVDSPSKLPA
                     INNIAEIREPIAECNMLVPQEFLGNVITLCVEKRGVQTNMVYHGNQIALTYEIPMGEV
                     VLDFFDRLKSTSRGYASLDYGFKRFQTADMVRVDIMINGERVDALAIIVHRANAAYRG
                     RELVEKMRELIPRQQFDIAIQAAIGNHVIARSTVKQLRKNVLAKCYGGDVSRKKKLLQ
                     KQKEGKKRMKSLGNVEVPQEAFLAILHVGKD"
     gene            34869..36098
                     /locus_tag="PXD71_000235"
     CDS             34869..36098
                     /locus_tag="PXD71_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDL6290404.1"
                     /translation="MQFREINPSPRLLMCPGPTDADPRVLRAMSAPILGQFDPEFTSL
                     MNETMEMERRLFQTKNEQTYVVNSTSRGGLETVLTAAICPGDKVLIPAFGRFGYLLNE
                     ILARSGADITIIEREWGTVFEPEEIEAELKKGGYKAVAIIHGETSTSMMQPLEEIGKI
                     CKQYDAMLIVDTVATLGGVDIRVDEWGIDACIGGTQKCISAPSGTALITYNKRIEEII
                     AKRKRVEKGIRAESDVDGTLPPIPSNYLDLAQLQDYWSPRRLNHHTETTSAQYGVHEA
                     LRIILQEGLEARFARHILNDKALCAGLEAMGLKIFGDRKHKAPVVTAFHIPEGMSGPA
                     LRSDILNHFGIELATSFGPLDGKVIRIGNMGFSSQKRNVLLTLGALEAVLLSHKVKVP
                     SGDAVAAALAVYKEADK"
     gene            36292..>36828
                     /locus_tag="PXD71_000236"
     CDS             36292..>36828
                     /locus_tag="PXD71_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6290405.1"
                     /translation="MPNFSFTHLYKKHTKELPTVTLLSALIGLSQVSYANPLPLGNTT
                     GTNNLAVGEGSFALGTGSQAIGKNAIATGGNISPQEFNVELENFRDLVNQIEELRTQI
                     AEQEKQGSVNQQLQDALKSQIAQYDTILERVREKQAQQAILANSVISMRKELVNRRNQ
                     LTELENNFNTSFLTGKNKQ"
BASE COUNT        10648 a         7992 c         6831 g        11357 t
ORIGIN      
        1 gtaatgtccc ttttttgaat gtctactaca aaatgggata ttttacaaac cgattgacta
       61 atgaagcaag aatataatta actacaactg gatatatttt gcaaatttcc cgataaattt
      121 caccgcttgt agataaaaac cgtagataaa aaccatagat aaaaaatagg agctaaacat
      181 ttttagcccc taccgtcatc agaaataaat cccgactaaa atcgccacga acacacctaa
      241 gccgaaatag tggttattta gaaacgcttt aaagcattcg ctacgttctc tttttttggt
      301 cagccaacat tggtagataa acagcgtggc acttaaggct aatacgacaa aatagccgat
      361 gtgataatct tttaccacgc ccaataaccc caataaaatc aaggtgataa actgtaataa
      421 cgcaataatt ttattatcgt attgagcgaa taaaattgca gtggatttaa ctccaattcg
      481 taaatcatcg tcacgatcaa ccattgcata ttgggtgtcg taggcgactg tccacgctag
      541 attggcaaaa aacagcagcc agcattcaag tggaagagtt tccgttaccg caccaaatgc
      601 catcggaatc gaccaaccga atgccattcc aagcactact tgtggcaagt gggtaaaacg
      661 cttcataaag ggataaatca ccgccagaaa aactgccaca aaggaaagca gaaacgcata
      721 gcggtttagc attaaatcga gcagaaaagc gaacaacagc aagatggcaa aaatgacttt
      781 tgcctcagtg gtggtcactc tgccggtagc taatgggcgt tgagaggtgc gtttaacgtg
      841 tccgtcaatt tctctatcgg cataatcatt aatcacacaa cctgccgcac gcataacgat
      901 taccccaagg gtaaaaatgg ctaaaatcgc aacaggcggc attccctctg cggcaataaa
      961 taacgcccat agtgtggggt gtaataataa taacgtgccg atgggtttat cgaaacgcat
     1021 taactgggcg taggcaatcc atttttgttt agaaaaaaga gtttcgttat tcattacaaa
     1081 ttttgacgat aacgtccgat tggcttaaat gagtctgtaa ttctaacggt aaatcttgat
     1141 ctgtaaacaa ataatttact tctttaacat ttcctaaacg tgcaatagct gtacgggtaa
     1201 actttgaatg atcggtcact aaaaccactt gtcgggaaca ttccattaat gcacgcttta
     1261 cctgcacttc gtggtaatcg taatcgagca tagagccgtc catatccacc gcactaatgc
     1321 ctaaaatccc aaagtccaaa cggaattggt taataaagcg aaccgtttct tctccgatta
     1381 gcccgccatc acttcttagc cctccgcctg ctaccgtaat ttggcaatct tcattttgca
     1441 tcagaatatg agccgcattt aaattattgg taactacttt taaattttta tgcgaaagta
     1501 aggctaatgc cactgcttct gaagtcgtgc caatatctaa aaataatgat gctccatttg
     1561 gaatcagttt tgccacctgt tgggcaatcg catttttctc gtgtgagaaa aattgcttgc
     1621 ggtgggtgta atcactattc tccgtattgg aaggagcggc tgccccaccg tgatgacggc
     1681 gaatcagatt attttctgcc atttcattca gatcacggcg aatggtttgc ggactcacat
     1741 tcaattcagc cactaattgc tcggtactca catagccaag ctgattaacc agctcaatga
     1801 tctttttgtg tctgtttgcc tgtttcatat ttcctctttt gcaaataatt tgcagaaaaa
     1861 gaccgcttgc aagcggtctt ttttcagtta aattttacaa tttatcaaca agttcaaccg
     1921 catagccgat atagcttgcc ggtgtcatct ctttcaaacg atttttttca ttttccggaa
     1981 tttccaactt atcgataaac tcacgcattg cggtttcatc aacccgtttg cctcgagtca
     2041 gctctttaag tttttcatac ggtttttcaa tgccataacg acgcataaca gtttgaattg
     2101 gctctgccaa tacttcccag ttttggttga gttcatcacg caagtgttgt tcattcactt
     2161 ctaatttgct aatacctttt aaggtggacg cataagcaat taacgcataa cctaaaccta
     2221 cacctaaatt acgcaacacg gtggagtcag ttaagtcacg ctgccagcga gaaatcggca
     2281 atttttgccc caaatgagcc attaccgcat ttgccaagcc aagattgcct tcggaatttt
     2341 caaaatcaat cgggttcact ttatgtggca ttgtgctaga gccgatttca ccggcaatag
     2401 tgcgttgttt gaagtgattt aaggcaatgt agccccacat atcacgatca aaatcaatta
     2461 aaatcgtatt aaaacgagcc acgcaatcaa agaactccgc aatataatcg tgcggttcaa
     2521 tttgggtggt gtatgggttc cacgtcacac ctaacgattc cacaaattct tggctgaaag
     2581 tgtgccaatc aatctccgga tatgctgata aatgtgcgtt atagttgccc actgctccat
     2641 tgattttagc gagaatttct aaattttcta actgtttata ttggcgttgt aaacgatatg
     2701 ccacatttgc catctcttta cccattgtgg tcgggcttgc cggctgaccg tgtgtgcgag
     2761 aaagcaacgg aatatgttgg taacatttcg ccagctctgc tacagcatca atcacttttt
     2821 tccattcagg taataatact tcttcacgag cggttttaag cattaaagcg tgcgaagtat
     2881 tattgatatc ttcagaagta caagcaaaat gaataaattc attcaccgct tgtaattcag
     2941 gcaacgcttc acatttctct tttaggaaat actccaccgc ttttacatcg tggttggtgg
     3001 tacgttcaat cgttttaatc cgattggcat cttctaacga aaaatcagcc acaatttggt
     3061 ttaggtaatc gtttgctttt tcagaaaaca ctggaacttc attaatttgt gtatgcgatg
     3121 ccaatttttg taaccagcga acttccaccg tcacacggaa ttttaataag ccaaattcac
     3181 taaaaatcgg acgaaggctc gcaactttgt cttgataacg tccatcaatc ggggataacg
     3241 cagttaaagc ggtaagttcc attgttttat attctccaaa taggtaaatg ttcggttaga
     3301 tttgacgata aaatttctgt gcttggttaa aaattttctt gcgtgaaaat aaaaattgcc
     3361 aacggcttcc gccgacttgc tgccacaaaa taccggctcg aataccggct agtagcgagg
     3421 ctcgaatttt attctgtata tccgggcgaa ccaagtaatc ttgcattccc atcacgtgaa
     3481 ttttcgtacc taacgggctg ataatatcgc tataaatagc ggctaaattg gcaatcattt
     3541 gatctgccat cacattatct tcatacaggg ctaactggcg ttccacttgc tgtaaacgct
     3601 gggctaactg ttgttttgct tcagggtttt tattgagttt ttggctaagg gcgagcaggc
     3661 ttatccaata acgtccgact tcggtatcta atttgccgtt accgccgcca gtttgagcta
     3721 atgcggtttc taagcccatt tttaaatggg aaagatcatc gccaaataca cttaacgttg
     3781 attccggctg agtgattaac aggctttcta aagcacactt aaaagcctct ctgtctgcca
     3841 tgcctttatg tgcaaattgt tgcactaacg tcgccgcttg gcaaacgccg gcataagcaa
     3901 gggtaatatc gtggtaatcc gccatctctg taaaattcaa tatgataaaa cattagtggg
     3961 aatatagcat tttttcagct tcttttgaat ggcaaaaggc atttattcta ttgaaaaata
     4021 aatgcctttt cattaatacg caatttgctg caaaaactat cctaccaatc gcccattatc
     4081 tattcgataa accacatcaa atttatccag tccgctttgg cggtgagtga tcataattaa
     4141 ggtgcgatca cagcagtgag tcaaaattag gtttaaaatc tgctgttcgg tttctctgtc
     4201 cagtccttct gttggctcat caagtaagac taattcagca gaactcagga gcaaacgggc
     4261 aagccctaaa cggcgttgtt cgccaccaga aagcggtctg ccgccctcgc ctaaccataa
     4321 atttaagccg tctgtttcaa gcaaatgccc taagcctact tttcttactg cttcttgcat
     4381 ttgttcgtct gtagcttgag cattccccat taataaatta tcttttaggg tttggctaaa
     4441 aatatgcact cgttggctta aggtaactaa ttttgagcgt aacatcggct ctgaatattc
     4501 gtcaatttta caattattca gccaaatttg accgcttgta gggtcgtagt tgcggtttaa
     4561 cagttggaat atggtcgatt ttccgctacc cgttttacct aagattgcca ctttctgccc
     4621 ttttaatacc ttaaaggaaa gattatttag tacgttttgc tcgccgtaat aagcaaagct
     4681 cacgttttca cagcgtacaa gcggttgatt ttggctcaaa ttttgccatt ctgccttgct
     4741 accaaatgtt acattaggct gttgttcggt aatttcacta atccgctcgg cggcggtaat
     4801 cacttgccct aaatgcaaaa aggcgatacc aatcggggaa agaatctccg ccgatgccat
     4861 tacacagaaa atcactaacg caattaaggc ttcagggtat tttgcagtgg gcacattaat
     4921 cgaagttgaa accaaataga tcaccacaca agtcaaaatg ccgttggata acattatcaa
     4981 gctatttgat aagccggaca gtcggctttc tttgctttga gcgttcagcc attgttgttc
     5041 agtttgctgt agtttttcgc tcatttggtt caagttgcca aagagtaaaa attctgcatt
     5101 taactgtacc cactcaataa attggctgcg gtaatttgct cggctttgta ttgcattacg
     5161 ccctaatttc aaacccaatc gataaaatac ggtaggaata acggcaagca ataccaatag
     5221 tgtgccgcaa atgatcagca ttaatggcac ggaaacaaaa gaaagcccga ttgccataaa
     5281 tgcaataatc atcactgcac tgacaaaagg cgagactaaa ttcagatata gggtgtctaa
     5341 cgtatccaca tctgccacca agcggtttaa caattcgctg tttctaaagc gatttagccc
     5401 gcttggactc agtggaatca attttttaaa gacgctagca cgcaaattcg ccaatacccg
     5461 aaaagtcgca tcgtgggtga ctaaacgttc aaagtaacgt gaaattgtcc gccctactgc
     5521 caaccctctc acccccgatg aagggtagaa aaagttaaaa ataagcgcat tgccggctag
     5581 aaatgaggca gctaaaaacc aaccggaaag gctgagcaaa ccgatacttg ccgctaatcc
     5641 taaaattgcc agcacaacac ctaataatag tcgcccaaaa tgggtttggt agagagcaaa
     5701 aaacgggaac aacgatttca ttagatttct ccctgatgca ctaattcatt atgtattaat
     5761 tccgcaaaaa agccggtgtg ttctaattca gtgaaacgac ctttctgaat gatttgccct
     5821 tgtttcatca cccaaatttc atcgcattgg gttaaatctt ccactcgatg agtaatcatt
     5881 agcgttgttt gctctcggct gagattatgc agagcggcta acacttgctg ttccgaatcc
     5941 atatcaaggc ttgcagtcgg ctcgtctaac agtaataatt cgtacgggcg aagcaacgct
     6001 cgtgcaatcg caatccgctg tgcttgcccg ccagagatac cgatattact gtcttgcact
     6061 tgatgctcca accctaaacg tgccacaaat tcatcggctt ttgaaagctg taatgccttg
     6121 gctaattcag aatctgtcgc attagggttg cctattaaaa tattctcttt caaactgcca
     6181 tgcattaatt gcggattttg ccccacccac gctaatttcg ctcgccattg gcttaaattt
     6241 aaatctcgca gttcggtgcc gttaatcgta accgaccctt cataaggcaa gaaaccaagt
     6301 aacatattca ttaatgaact ttttcctgca ccactttgcc caaccaaagc gatatgttgg
     6361 tgtgcattca actcgaaatt taacgcttgg gttaaggctt tgccttgtgg tgagaagatc
     6421 acgcaatctt tggcttgaat cacaagcggt tgattttcaa cattttcttg caaattgcca
     6481 ccttgcgttt tcaccttttc ttttaagaaa gtttcgatat tatctgcggc actaatcgct
     6541 gctgccttgt cgtgataaaa cacgccaagt tcacgcatcg gttggtaaaa ctcaggtgcc
     6601 accattagac aaaagaagcc gataaataag gtcacgcccg taccataata gccaaaatcc
     6661 agctcaccta aaaaaataaa gccaaaatag accgccgtca ccgcaatcga taccgccgtg
     6721 aaaaactcca ataccgcaga ggataaaaac gccattttta gtacgtccat cgtactgatt
     6781 cggaaatctt cggtgctttg gtagatttgt tcagtctgct tttccgcttg cccaaataag
     6841 cgaatggttt ctaaaccttt taatttatct aagaactgtc cgctaatacg ggaaagaatg
     6901 ccgatattac gctgatttgc ctccactgct ttcattccgg ctaaaatcat aaataaaggt
     6961 aataatggca ttgttgcaaa taaaatagcc cctgctgccc agttaatcgg gaatacaaaa
     7021 cagaggatca gtaatggcac tatcagcgat aaaaattgct gtggcaaata gcgtgcgtag
     7081 aaattatgta gattttccac ctgctcgagc attaaggttg cccagctgcc cgccggtttt
     7141 tgttgaatgc tcatcggccc gactgcttcc attttggcta gaatttggcg gcgaagatgt
     7201 aaacgcaagg tttgccctgc tttgaaaccg acacgctcac gcagaaaaat gaagaatgct
     7261 ctggcagcaa aacaacagaa taagagcata atttcaacca gaaagctatt cggtgactgc
     7321 tttccgataa gcattttgtc taacattgtg gcaatcagcc acatctgtcc aatcatacat
     7381 aaactactga aactccctaa cagtatgttt aagtgcatat attttttgat gactttttgc
     7441 tgacttttaa gccatttttg taattcgcgt tggcgttgtt tttccataat tttgtcaatt
     7501 tttttgtttt ttcaaccgct tgtaaatcgg gtttgccatt ctactataaa gcaaaaaggt
     7561 tgttaatggc gaaacattaa caacctttca ttcagcctta actagaatag ctcgtcaaat
     7621 tcaaagcgat acattaaatc taccgcttgg ttaatacttg agacccattg taagtacaat
     7681 cttggcgtta aattatagcg taaggttaat tcggtaagcg gtgcaaaaat gcccacgcca
     7741 tacttcactc tgaatttagg ggcaaggctg gcacttactt ctactttggt gttatcgcca
     7801 atacctgcgg tggtcacgct taaatctttg agtccgaaag tgctaccaac atcacccacc
     7861 aatttactgc tttttgataa gctcatactg agtaatgccg ctgcgactgc attactggag
     7921 ctggtatcgc cactgctttc caatgaacga ccagttaaaa tataggaaag tgcttcattt
     7981 tgcgacattg ccggatcaga gaatactttt accgttggat tgtctgccaa accggtcact
     8041 cttaccccag ctgtaatggt cggatcttcc attgcctccg gattgcgtat agcttcaata
     8101 tctaaggttg gctgagacgg tgagccggca aaactaatat cacctttacg aatcagcaag
     8161 tcttgaccat aagcggcaaa tcgtccttca cgcaaatgaa cttgcccgta aagccctaaa
     8221 ccttgtttgc cttgcctgac agacaataag ccattcacgt tactgtttaa accataggct
     8281 ttgagtttga catcattacc gatgttaatc ttaatatctg cattaattgc cattccacct
     8341 gaggtttgag ccggaatatt acgttggcta aacggcactt tatttttcac agagccgtcc
     8401 ataatcacct catctccact tacactgacc gcactttcag gtaactgctc gacttcaatt
     8461 cttgcccaag ggatatctac atttccactt aaggttaaaa gagttggcgt tgccgtcact
     8521 tgaatatccg ggctaactgc cacttttgcg atattaggaa tatccacttg gaaacggtta
     8581 gcttgagcgt gaacccgtgt tttccacgca tttagatctc gccagtctgc atcaccctcc
     8641 aaacgcagtt cgctttgttt ggtttgtaat ttaccgctta aagtcgatgt tgccccgtgg
     8701 aaattcattg ctaaattccc acctgtaata tcaaacggca ttgtaacaga gcgagctttt
     8761 aactctgtaa cgtttagatt tccgtgcaat aacggagagg ttgcggtgcc gctcacggta
     8821 agacgagcat tcacattgcc atcaaccgaa tcgccccgtt caagtagcgg tcgaatcagc
     8881 tttaagctta attggtcaag atgaatattg ccggataacg cacgagcctt acttaaatct
     8941 ttcatcacta aatcagtcga aagtctgccg ttattttcga cccttaaatc agatttaagg
     9001 gttaaattat tgtcagacat tttcagatca attttaagcg gtgaaacggt gagtgggaaa
     9061 ctttttcccc cctccatttt ttgaataaat ttaatcgcat tagaggttaa atccagattc
     9121 acttgaggct gtttgttttt aaaccaagct gcatcgcctt tggcattcac aatccctgaa
     9181 agctggctgt ttttctccaa aaattgttgc aataccgcta aatcaaagtt acggatttca
     9241 aaaggcactt taccctcaac accggcatta aatgcagtcg ggaaacaaag attaattttc
     9301 cggttatgcc aacaatgtgc ggaaatattg gcgttaatgg ctttattgtc atatttcaca
     9361 ttcactgctt tatctgcttg gaattgacca aactcggtgg attgaatcga aacttggctt
     9421 aattgccctt cccaaatttc tttcaaacgg tcaaattttc cagaaatttg caaatcagca
     9481 ccaaccggat tgccttttgc acttaatttc aagctatgat tttcttcatt tccttttagc
     9541 aataaagtgg catttcccac tttgatttca ttataggcaa attgacgtaa ccccaaggtt
     9601 aaatcccctt gaatggtttt ctcagtggta attttgcctt ttgcggttaa attttgcagt
     9661 ttaaattgct catagcctac atttgaggcc actaaatcta aatcaatgtt aggttctgaa
     9721 accttacccg ataatttggc attaccattc acgctggctt ttaaattcgg cactaaccct
     9781 tgcaaattag gggctttaat atccgcatag aaatcagatt tttcgcctaa cacgcctttt
     9841 aaatcgatct tattttcacc atacaccagc gataagcccg gaacatctaa aagttgttga
     9901 tgatcggctt ttaaattacc gcttaagtgt agatttttct gtaataacga acctttaata
     9961 tcaatattcg agacgtccgc tttccatccg ctagctgaat tgccccgtcc tgcataacct
    10021 tcggaagata aagaaccgga caataccgcc ggccagtctg ccagtaggga tttagtgtta
    10081 atatgctgaa gtttcaaatt tgaacgccac gcaacgccct cgctccaatc cactaaaccg
    10141 tcgatcttcg ccgtaccttc cagcgtattg agacttaaat cttgtatatt aaaatgggtt
    10201 aattcccctt tgccagttaa actagccgag cttgcaggca cgcccattcc tttggcactg
    10261 actttagcct ccaaagcgta atccaggaga ttgccattaa gcctcatggc gatattctct
    10321 aaactcagca catcaccatt ttgctgagga gcaaacggat aacgcattga ctcactcttc
    10381 agcgataaat caaagggagt tttcgcctga gccagtgcaa cattgccctc caacgaggcg
    10441 ctaattgcac cagccgtctt cacttttagg ctggttttgt catatagctc accggaaaga
    10501 gaggcttcaa tttgattgaa cggcaatttt actttcgccg tttttgcctc gtgaccactt
    10561 agcgtccaac ttagcggata attatcttgt aacgataaag aaccgcttgc ggttagatcc
    10621 ccttggtcgc ttttgaaatc taaacttttt aggttgatat gctgatcttt tgcttgagcc
    10681 tgaagctgta aagaggcaac atttaacaat gagatcggct caatcaaatt ccctttttca
    10741 tctttagctt tttgagcaat cgctacattc gcaatatcaa tctgttcaat attgagatca
    10801 agaggcaaac taaagcgaat atttttatcc aataacggct gtgctaattg tgctttaatc
    10861 gctgcccaat ccactttatt atccacctgt tcggcctgtg caacagtttt tctctgtaat
    10921 tggtgaaagc cctctacgac tttttcactg gttttttcca ccgcctcttg aatcacttta
    10981 tcagcaatct cttgtggatt tgtcgctgat aacgcctctg tttttgcatt tttttgtgca
    11041 aaatcgaccg cttgttcagt atcagtttcg tctaacttta aggctaaaag cagatttagc
    11101 ccctctaact gtgtcgggtg aacggtaata gcccgccctt taccagaaag tgcagtgtgg
    11161 aaatgattaa gatgaatatc catatcatca actttgacct tgatattctc taaattgata
    11221 ttttgagctg aaatccccaa tggcaaattt aattcggtga aaggttcggt ctcttctttt
    11281 ggctgagaag ggggtaattt agtcgtatca atcaacacct ctgtatcttt aaggctgaga
    11341 ttattcaaac acatttcata gtggagcaag cagctaaaac caatatgcag atctgcttgc
    11401 ccaaccgtta cattcacacc gtccatcaca aatcgggcat cagaaagctg taacccatct
    11461 tgcaggctac cttgcacttg cccaagttgt aatggctcta cgaatttatc tacccagctt
    11521 aacactttac gttgcccgta acctgtgcat agaaagagaa tggcgagtaa aagtagaata
    11581 aacgtcagac aaaagccgtg cttcaaaaac cgccactttg attttttagg ctgtgttgct
    11641 tgtggctctt gtgctgagtc cgtttgattt gtggctgttt gtttattttc gtcagtcata
    11701 atcgtgcata tttatgaggt gataaaaagt aaacaagcgg tatgcgaaat tttactgaaa
    11761 ttcgaccgct tgttttcatt tacagatcgg aacctaaacc aatataaatt tgtattcctt
    11821 tggaattatc tggtgatttt accggcgtgg cgacatcaaa tttaatcgcc ccgataggtg
    11881 atgcccaacg cacccctaag cccgtaccgt agtagagatc ctgagttttg aatttattcg
    11941 atgccaagcc tgcatcataa aaaattgcac cccaccaatt agggtaaacc tgatactgat
    12001 attctactgt gcctgtggct aaatgagagc ctccgacacg tttgccatta ctcggatcac
    12061 ggggggaaat atccttatag ccaaagcccc gcacactcat atcgccaccg gcaaaatagc
    12121 gtaacgccgg tggaatacgt tcaaattgat tggctttcaa ataccccaac tccgccctta
    12181 aatagaaccg atggttatca aagtaagttc tcgcccaaga tgtcgaggct ttataactat
    12241 aaaagtttac atctgaacct actgcacgac tcccccagtt taccgttaat ttttgtgtat
    12301 ccccccataa tgggtaaggc ttgccatctg ttctggttcg attcaatgat gccgttgggt
    12361 aaactaataa ggttttaaat cgatcattag cttgagtaaa agaatcgtaa cgggctttta
    12421 aaccaacaga aaatgaccaa ccggtttcgt gagtccagaa acgttgtaag ccaagggtgg
    12481 cagcggttga ttttgtatcg ttttgatctt cgttttctaa agtgccggat aattggtaat
    12541 aataatgtag cggatcttct ctaagtggaa tattataacc aaattcaaat gtctgctccg
    12601 gcttagaaac gtacgttcgg ctttcaatac tatgacctcg gctatttaac caaggtttct
    12661 tccagttaag ctgtaacctt gggccaactt cagtttcgta gcctatccca atttccactt
    12721 cattgcgttt tttaggctga aataacacat ttaaatccac taatttatcc tgttcattta
    12781 aatgtgactc aaccatcact gatgaaaacc aattactgga aaggtaatct tgggttaaaa
    12841 tcgaaagatc gttagctaaa tatggctcac cggaatgaat tttaagaata ttttgcaaat
    12901 attcttcttt gatttggcta tcaacaaacc ggatttgtcc ataatggtat cgttttccgc
    12961 tatcatagcc taaacgccaa tccgctttat gatcggtcgg ataaacttcc aaacggtgat
    13021 aaagccaatg accgtcaaaa taccctttac taaaagcggt actttcaata tcacctttaa
    13081 aattatcata ggttttatgg tttaaacgac taccttgttt tggggcttta ctgactagat
    13141 gagcgaaatc ttcgtccgtt tttgcatcgc cttgcaaaac aatatcggtt tcatcaattt
    13201 ttaccgtctc tttatctagc tcaacattta atactaagag cggtttcttt ccctcttgag
    13261 gtagctgcac aaattggtat cggctattat aatagcccaa ggctctcaga gctttatcta
    13321 ctgtggtttg caccaaatat tgataacgct ctgaaccatc tgcatattca ttggcaattt
    13381 gatctaaata tagctccaca tttttcttgg catctttacc ctgaatgcct ttgacttcta
    13441 aagtgactaa tggctctatt tctggctcat catcactatc ttccgctaat ttttcttgca
    13501 gattttcaac tcgctcacga tctacgtcag acagcctttg ctcttgattt gcttgtgcca
    13561 ttgaagcaaa agccactaaa cagcagaatg ttaaaaaatg tagttttgta ttcattagta
    13621 acctttttat tcttttggaa tgccacgcaa aagcggaata ctgtcataag gcggaacaga
    13681 acttaacgct ggctcttgct caaagagcat aatgaatggt ttgtcaggca ctgttttttc
    13741 attacgccac aagtttccaa cactcaccaa ttcaatatct tgaggtagtt gtgctaaatt
    13801 ttgcttcagt acactaatcg tattcttgcg agggtgtcct atcaccaccg ctaccccgtt
    13861 ttttcgtgcg tgattaattg catgtgcaaa ctgtttttgt acatcagcaa gcagatcgct
    13921 atcatctaaa aaaatgtctc tctccagcgt attaatacca aattgacgag ccgttttagc
    13981 cgccacacta ctgccaatag ttttactgtc taaaaagaaa agcgtatttt cttttaacac
    14041 tttcaacaaa tgttgcattg tagcagagtc tgaagttgct ccactgccca tatggttatt
    14101 taaaccaata gcatatggta cttgaccacg agaagtatga ataagtttcc gaattttctc
    14161 ttcgttatca ccaatatgaa tacccccctc ttcaattggc tgtttcgatt taggttccat
    14221 tggcaaatga atcaaaatat cccgtttttg gttataagcg tcctttgccc gttctgttgc
    14281 atagggtgca acaggaataa tcgccacgct taccgccttg ggtaaggcta agatttctcg
    14341 atcttctttt attcgatagc ccacatcatc aattacaatc gccatttgag ctgcttgtac
    14401 aagcggtgta aaaagcacaa acatttgcaa aaaaatgtta aaattcaacc gcttgttaat
    14461 ttgtttaaac atctatttca ccaaatttag aggattaatc ggcgtgcctt tacgggtaac
    14521 accaaaatag agtgcagaac ggctttgtcc cccactgtta ccaaccttcg caataacctg
    14581 tccgccttgc actcggctac ccttgctgac tgaaatagat tggttatagc cataaaggga
    14641 aatatcacca ttgccgtggt caatcgcaac catattgcca taccctgcaa gccattgagc
    14701 cattatgact cgcccgctcg ccattgctct aaccgaagta ccggcagaag caccaatcac
    14761 tacaccgtgc caattattac caaaaggagt gataatattg ccagacaccg gcatcgtata
    14821 tcgaccagcc ttgatcggtg ttctctcaac attctcagtt gttttggact gaccggattt
    14881 tgcctgtttt tcccgctgtt gttcacgttt ttctgcttgc tgagcctctc tttgcgcttg
    14941 ttctaattgt tgttttagtg cctgttcatt gctgcgtaaa ttctctaatc ggctttgatc
    15001 ctgctcaagc gttttatcga ttaaacgtaa ggtactttca cgctggcttt gtactttctt
    15061 taaatctttc tcttgtttct tttgttcatt aagttgcgtt tgatgatctt tttgctggcc
    15121 tttcaattca tcacgtctgg ctttaagatc agcttgggtt ttacgaattt caacgatcgc
    15181 atcaatgcga atattattca tatgctgata ataagcggtc attctttccg cattttttgc
    15241 ctcttcagac attaaacgct ctaagaccga agggttcacc ccagaacggt aggcagagtc
    15301 caactgctct tttaattttt ctttctgctg tttttccaac ttttctaaac gggcaatttc
    15361 agtctcagtc gctttaatgg tctggcgaac ttccgttaaa gacatttcgg ttttttgtaa
    15421 tttatccaac accttcccca tttctacttc ttggtttttt agggtagatt gcaacgcatt
    15481 acgtttttgc ttttgttcat taatttttga ggtttgctgt tggatttttt gctgaatctg
    15541 attaagatta tttgcaaaag aaaaaggggt agaaaagctt attaccccac tgaaaatagc
    15601 agtccaacag taagaattta ttcgtttcac cagcatacct ttgtttcttc aaaatattat
    15661 taaggttata ttctaatcgg ttatttgctt aagatcagct ttttagtgaa agaatttaac
    15721 tatctatttc aaaatataaa gaattttgct ataaatagca caatttcaaa tccattaatt
    15781 ttactcaaag ctaaggagtc attatgcaat tagtatttat ccgtcacggt ttcagtgaat
    15841 ggaatgctaa aaacttattt accggttggc gtgatgttaa tttaacagaa cgtgggattg
    15901 aagaagcaaa atctgccggt caaaaattaa aagcagccgg ttttgaattt gatattgcgt
    15961 ttacttcggt tttaacccgt gcgatcaaaa cttgtaacat cgtattagaa gaatcagacc
    16021 aattatggat ccctcaagtg aaaaactggc gtttaaacga gcgtcactac ggtgcattac
    16081 aaggtttaga caaaaaagaa actgctgaaa aatatggtga tgaacaggtt cacatctggc
    16141 gtcgttctta tgacatctct ccaccggatt tagacccaca ggatccaaac tcagcacata
    16201 acgaccgccg ttatgctaac ttaccaaaag atttaattcc aaatgcagaa aacctcaaaa
    16261 tcacccttga gcgtgtgtta ccattctggg aagatcatat tgcacctgca ttaatttcag
    16321 gtaaacgcgt attagttact gctcacggta actcattacg tgcattagca aaacacatcg
    16381 aaggcatttc tgatgcagac attatggact tagaaatccc aaccggtcag ccattagtgt
    16441 atgaactaga tgacaactta aaagtaatca gcaaacgtta cttataattg gtaaatttcc
    16501 gataaaaaat aaccgcttgt agtgcaacac attacaagcg gttatttttt gttaattttt
    16561 tgcaaatggc aaagaacaaa aataaaggcg aatcatacat tcgccttatt gttttaaagt
    16621 agattagaaa tctaacaact ctttctcttt tgctgctaat acttcatcaa cttttttaat
    16681 gaaggtatcg gtgatttttt gaatatcttc ttcagctttg tgttgttcgt tttcgctgat
    16741 ttctttatct tttaataacg ctttgatttg gtcattagca tcacgacgaa cattacgaat
    16801 tgccactttg ccttgctcac cttctgcttt aacgattttg attaaatcac gacgacgttc
    16861 ttccgttaat ggtggaagcg gaacacgaat ggttgtgccg gcagaagatg ggtttaaacc
    16921 taaatctgag gttaaaattg ctttttctac cgcagagatt aaagatttat caaatacgtt
    16981 taccgctaaa gtacgggcat cttcagctac cacgttcgct acttgacgta atggagtcgc
    17041 agagccgtaa tattccactt gaataccgtc taataaagaa ggttgggcac gacctgtacg
    17101 aattttcgca atatgccctt ttaaagcctc aaggcttttt tccatacgat cttgggtatc
    17161 tttcttgatt tcgttaatca ttgatttttt atccttttat ttagtaactg agagttaaac
    17221 agagaaaatt aattattcag aaatggttgt accttcttca gtaccgaata ccacattgcg
    17281 taacgccccc ggtttgccca tattaaagac acgaattggc attccgtggt cacgagcaag
    17341 cgtgaatgct gctaagtcca ttacttgtaa ttcttgctca ataacttgcg catacgttaa
    17401 tttattatat agttttgcat ctgcgtattt tgctgggtct ttatcataaa cgccatcaac
    17461 tttagttgct tttaatacca catcagcttc aatttcaata ccacgcaagc aagcagctga
    17521 atcggtggta aagaacgggc tacctgtacc ggcagagaaa atcaccacac gtttttcacg
    17581 caacatttta attgcttcag accagttgta agtgtcgcaa atcccattta attggaacgc
    17641 agacattaat ttagcattta cctctgcacg gtgtaaggca tcacgcatag ccaaaccgtt
    17701 cattacggtg gcaagcatac ccatatggtc gcccactaca cggttcattc ctgctttggc
    17761 taattttgca ccacggaata agttaccgcc gccaagtacc acaccaacct caacgccttc
    17821 agcaagtaat tctttaatct ctaacgccat acgatccaaa atagacgggt cgataccaaa
    17881 accttcactg ccttgtaggg cttcaccact taatttgaga agaattcgct tataaatagg
    17941 tttgctcatt ttaattgcct cttaactgta tggactaaac acaaaattac gttattatag
    18001 aacaatccaa tataacgata aactaaaaat attcactaca atgaaatcca aacaactttt
    18061 cggcattctt gtcgctattt cgctggcaac tgctgctagc tgtttaatgc taatcggatc
    18121 aggctttttt ccaaatccga gtatctttga agtcattttg ggcgtattcc tcatcatcgc
    18181 tcttgccagc tctaaatgga cttattattt actcttactg ccaatcagca tcgcttatgc
    18241 aatttatacc ccggttggga taaaattcgg taaaccgact tacgagtata tcgcctctat
    18301 ttttgctacc gatatgatgg aaagcaaaga gtttattgct caactgccga ttttaaatat
    18361 tgcggcgggc attgcgattg tactagcggt cattttttat cgaaaaatta ccaatcgcta
    18421 tcaaatccgt tttttacgca ataaaacatt cgtgatttcg gcttttgtat taatgctgtt
    18481 ttcccttgca ccgtttaaat tttttcagga gttctacaat tcgagcctaa aggtaaagaa
    18541 agaattggaa gtgctgaaca atttaacgct agattcacaa tggggacaat caaccctcag
    18601 ctcaacttca aactacgatg attatgtact gattatgggc gaaagtgcga gaaaggatta
    18661 tcaccacgcc tatggctatc cggctgccaa tacgcctttt atgagttcgg caaaaggaac
    18721 cttgattgac ggtttaacct ccggtggcac aaacactatc gcctcattac gcttaatgct
    18781 cactaagccc gatacggaaa aatgggaagc aaattatggg ttaaccttaa ttgatttaat
    18841 caaatctacc ggtatcaaga cctattggat ctcaaatcaa ggctatttgg gcaattacga
    18901 tacgccgatt tcctctatcg gtaataaaag tgatgtcaaa attttcacta aagccggcga
    18961 ttcattaaat accaatgtga gcgattttga attgttaccg cattttgcta aagtgattga
    19021 agaacctgct caagggaagc gtttcatctt tctgcattta tatggctcgc atcctattac
    19081 ctgcgatcgt ctcaccgatt acccgaaaat gtttgacgat gacaaactgc caaaacgcta
    19141 ctttaatgta aattgctacg tgtcatctat caagaaaacc gatgaattgc tgaaacgggt
    19201 atatgatttg ctagtgaaaa accaagaaaa tcaccagcgt tcattctcaa tggtttattt
    19261 gtctgatcac ggtttatcgc acgaggtttc aaaagagaac attgttatcc acaatggaaa
    19321 ggatagaagt aaattacact acgatattcc gctgtttaaa atttcaagcg atgataccca
    19381 acgtaatgag tataaagcct ttaaatccgg cttaaacttc actaatggta ttgcgaattg
    19441 gattggtatc gaaaatccgt tattagataa agaaattaat ctttttaacg aacaaaacga
    19501 taaaagtgat tatgggctaa agaaaatcat tgaaacgcag tctactgtag aagatccagc
    19561 tgttcctatt cctgaaagct acttatataa agcgactaat tagcaaaagt aagtaacaaa
    19621 aaagcaggct ttaagcctgc ttttttagtt tttagtatca gaaaaattaa gcgttaccgc
    19681 cagtgatttt cgctacttct gctgcgaaat cttcttctac tttctcaata ccttcaccta
    19741 cttctaaacg aataaagtta gaaactgaag tatttactga ttttaaatat tgacctacag
    19801 attgagatgg atccattaca aacggctgac cggttaatga aacttcaccg gtgaatttag
    19861 ccatacggcc ttcaaccatt ttttctgcga tttcttttgg tttaccagaa ttcatcgcaa
    19921 tttcaatttg aatttcacgt tcttttgcta ctacatcagc agaaacatct tctgggttta
    19981 caaattcagg tttgcttgca gcaacgtgca ttgctacttt cttaagctca tcttcagagc
    20041 ctgcacctgc tactaataca ccaattttcg caccgtgtaa gtattgagca ataacttcgc
    20101 cttctaagta ttgaacacgg cgaatgttca tattctcacc gattttcgca actaatgccg
    20161 cacgtttttc ttcaaattgt gcttgtaatg cttcgatagt tgtaccttta tttgcaactg
    20221 caaaatcagc aacttcgttc gctaattcta agaaaccggc atcttttgca acgaagtcag
    20281 tttcacagtt catttcaact aatacgccaa aacctgaagc gacacgagca aggattacac
    20341 cttcagctgc aacacgacct gcttttttag ccgctttcgc ttgaccggat ttacgcatat
    20401 tatcgattgc taattcaata tcaccgttcg cttcaactaa tgctttttta cattccatca
    20461 tacctgcgcc ggtacgttca cgaagttctt taactaatga tgctgtgatt tcagccattt
    20521 taaattcccc ttatttgata tggtaaaaca ggctctcacc tgcctagaaa tttcattgtt
    20581 tagggcaggt taaaacctgc cccctcaact gaattattcc gcagaagctt cttctgctac
    20641 ttcaacagtt tcaacaccgc gaccttcttt gattgcagca actgctgaat ctaagtaaag
    20701 ttggatagca cgtgttgcat catcgttacc gggaatgatg tagttaacgc catctggagt
    20761 tgagttagta tcaacaatcg caaataccgg aatacctaag ttgtttgctt ctttaattgc
    20821 gatatgttcg tgatcagcac cgataacaaa aattgcatcc ggtaaaccgt tcatatcttt
    20881 gataccgcct aagcttaact ctaatttttc taactcacga gtacgcatta acgcttcttt
    20941 tttagtgatt ttgtcaaaag taccgtcttg agtttgagtt tctaaatctt ttaagcgttt
    21001 gattgattga cgaactgttt tccagttagt caacatacca cctaaccaac ggtggtttac
    21061 gtagaattgt tggctctcta ctgccgctgc tttaacagca tcacttgctg cacgttttgt
    21121 accaacaaat aatactttac cattgttact agcaatgcga gttaattccg ctaatgcttc
    21181 gttgaataaa ggtaaagttt tttctaagtt gattacgtga acaccattac gagcaccaaa
    21241 gatgaatggt ttcatttttg ggttccagta acgtgtttgg tgaccatagt gaacgcctgc
    21301 gttaagcata tcgcgcatag aaacttgtgc cataagaatt ttccttcaaa gttgtttgtt
    21361 ttgccactta tgaacggcaa aacggggttt agcctccaca tatcctagaa aatcaatctg
    21421 ttaaagacac cccaattcct agcttgtgat atgtgtgtgt tttagtgtta aaaaataaat
    21481 gtgtacgttt tgcctgaata gacaaaaacg gggcgtatta tgccaaaaaa caaacaattt
    21541 gccaattatt ttattgctct cttcgcatta ctaaatcaag ctcaaaaata aaaaccgttc
    21601 tcattattga ctaaaaaaat ggggaggttt agaatgtctt tcattctgtt atgaaaacct
    21661 gtctgaagga gactatcatg aaaaaaacac tttctgcact tgctattgct gcagccactt
    21721 ttacatcaac ttcaacgctt gctgcaaatg aagtgaatgt ttattcttac cgccagcctt
    21781 acttaattga gccaatgcta aaaaactttg aaaaagatac tggcattaaa gtaaacatca
    21841 tcttcgctga taaagggttg gttgatcgtg taaaacaaga aggtgaatta agtcctgctg
    21901 atgtgctttt aacagtagat atttcacgcg taatggaaat tgttaacgct gatcttgcac
    21961 aaaaaattga ctctaaagtg ttagaaaaaa atattcctgc acaattccgt gactctaacg
    22021 accaatggtt tggtttaacc acacgtgctc gtgttatcta cacgtcaaaa gatcgtgtag
    22081 gtaaattacc agcaggcttt gactacctag atttagctaa accagagtat aaaggtaaag
    22141 tctgcgtgcg ttcaggcaaa aactcttaca atgtctcact attcgcggcg atgatcgaac
    22201 attacggcat tgaaaaaacc aaagcattct tagaaggctt aaaagcgaac ttagcacgaa
    22261 aaccacaagg tggtgaccgt gatcaagtaa aagcaattaa agaaggcatc tgtgattact
    22321 ctatcggtaa cagctactac tacggcaaaa tgttagatga tgagaaacaa aaatcgtggg
    22381 ctgaagcagc gattattaat ttcccgagtg gtgaacacgg cacgcacaaa aacatcagcg
    22441 gcgtagttat tgcaaaacac tcaccaaata aagcaaacgc ggtgaaatta attgaatacc
    22501 taagtggcga aaaagcacaa ggtttatatg ctgaacttaa ccacgaatat ccagtaaaag
    22561 aaggcatcga gccatcagca atcgtaaaag gttggggtac gttcaaatca gataccatca
    22621 aacttgaaga tatcgcaaaa aattacgaag ccgcattaaa attagttgat gaagtgaaat
    22681 ttgacgattt ctctgagaaa aaataatcaa ccgataagcg gtcatttttc cacaaaaatt
    22741 tgcaaaattt tgttaaaata caaccgcttg caaaaggacg cattttgcgt ccttttattt
    22801 ttttatgaaa taacaggctt aaagaaaaat gcgatcacaa ttttgctgga aaagtattgc
    22861 tgccctaaca acttttatcg ttttgcttcc tcttattgca atttgctatc acgcattgca
    22921 aggggataat gaaaaccttg ctcacctttg gcaaacaatg ttgccaatct atcttaaaaa
    22981 ctcaacttta ttagtattag gaaccgtttt ccttgcactg atttttgctc tgcctaccgc
    23041 ttggattgtg gcaaattacc gtttttgggg gcaaagaacc ttacaatggt tactttgttt
    23101 gcctctcgct atgcctgctt atttaattgc ctatttatat accgatttat tagattactc
    23161 aggtgctttt caaggctggc tgcgtagtat ttttggctgg actcatccgc aagattactg
    23221 gtttcctcaa attcgtacat tgtatggtgc ttgctttgtg cttgcgttag tactatatcc
    23281 atacattttt ttattagtgc gagttgcctt attagaacaa tctgaaaact taacacacag
    23341 tgcaaaaatg ctcggggctt cgccctctca gttgttccgt cgaattacgc tgccattaat
    23401 tcgaccttct gttgccgtag gcattgcatt agtggcaatg gaaacgcttg gcgatttcgg
    23461 tactgtggcc tattttgccg tgccaacact tactacggca atttatgatt cgtggctagg
    23521 ctatcacgat cttggtactg ccagccagat tgcgatcttt atgttgctaa tgattttcct
    23581 gtttttaagt ttagagcaat atagtcgccg taaacaaaaa agctatcaac gtggctatga
    23641 aaagaaatca gcctttaaat ttttaactgg ttggaaatta gcattagccc ttgcttggtg
    23701 ctggggttta ttggcgttag cctttttcat tccgtttggc aggttgctct attgggcata
    23761 cgactatttc gagcaagcgt ggaatttaga ctttgtccaa ttttcgctta atagtttgaa
    23821 agtatcgatc atcgcctccg tgataacggt actgattgct ctacttctgc atttttctaa
    23881 acgtttgaat acccgcaaaa ttttagtcaa aaatgaccgc ttgtcattac aacttgcctc
    23941 actgggctat gcggttcctg gcacagtatt agcgatcggt ttactttcgc ctttaacatt
    24001 tgccgatcat caactccatg cttggctaaa aagccttgaa ttatcacccg ttggattgat
    24061 tttttcaggc tcactctttg cattagtatt agcttatacc atccgatttt tagcaatggc
    24121 aattggcagc cttgaaactt cgctaaataa aatctctcca tcgctggata tggcgagcca
    24181 aacgcttggt aaaaatggct cagcaatgtt agtaaaaatt catatccctt taatttataa
    24241 aggtatttta actgcaggat taatggtctt tattgagagt atgaaagagc taaatgcttc
    24301 tctgctactt cgcccattta attttgatac actcgcaacc tatgtgttta cctttacatc
    24361 agacgagcag cttgagcgtg ctgctctgcc tgccatcgta ttggtgttag tcggtttact
    24421 gcctgttatt tggcttacca gatcccttat ttcgcaatct gaaaaagaaa gataaaggaa
    24481 ttttatgtct gttttaacta ttaatcatct caacctcaat ttcggcagta caaaggtttt
    24541 acaaaatctc catcttgctg tgaatcaaaa tgaaattatc tgtttgctag gtgcaagtgg
    24601 ctgtgggaaa actacactgc tcaaagccat tgcaggttta ttgccaattg agcagggaga
    24661 tattcattta gcgggtgaat gcctaaaaag taaagccgtg gaagagcgac aaatcgggct
    24721 gattttccaa gattacgctc tttttccaca cttgacggta gccgataata ttcaatttgg
    24781 cttatccaaa ttgccaaaag tacaacaaaa agcaattacc gaaaaaatgc tttcagttgt
    24841 gcatttacaa ggctttgaaa gccgttttcc acacgaactt tcgggcgggc aacaacagcg
    24901 tgtcgctatt gcccgagcat tagcctgcca gccagaattg ctactgttag atgagccgtt
    24961 ttccaatatt gacagccaaa ctcgctatgc aatgattcaa gagatcaagc aaatcttaaa
    25021 aagccaaaat gtgcccgcta tttttgtaac ccatagcaaa gaagaagcct ttgcatttgc
    25081 cgataaaatt gccgtgatgg atcaagggaa aattattcaa tttggtaagc caaacgagct
    25141 ataccattct ccagttaatc catttgtggc agatttttta ggtggcacaa actatcttaa
    25201 ctgtaatgta acggaagaca acgtgttatg cagccctatt ggcgagttcc gactcttccc
    25261 tgaaatgaac gttcagaaag ggcgttaccg ttggcttctt cgcccagaac agattatctt
    25321 aaaacccaat gagctaggtt taggtaaagt ggtaggcaaa ctgtttttag gcctatacta
    25381 ccgctatcag gttgaaatta aaggcattga gatagtaact taccaatctc aagagctgaa
    25441 aattggcgaa caagtggaag tgggcttcca agtaagagag tttatcctat tttaaggaga
    25501 atatatgaaa attgaagttt ggtcagatta tgcctgcccg ttttgttaca tcggcaaacg
    25561 ccacttagag caagccctag cccaatttga aggcgaggtt gaagtggttt tccgagcctt
    25621 tgagcttgat ccgcacgcta acggcgaacc ggagggcgat attcagcaac gcctaatgcg
    25681 gaaatatcaa aaaaccgcag aacaagctga tgaaatgatt cgttatgtgg aacaagccgg
    25741 caaacaagct gggctagatt tgcgttaccg caccacccaa tacactcgca cctttgaagc
    25801 tcatcgcttg gcgaaatttg cagaaagtaa agacttaggc gaagcaatgg ttgaacgttt
    25861 attcaaagcc tattttaccg ataacactat tctagctaaa cgcacagagc ttatcagttt
    25921 agccttagac attggcttag agcgtgatga aatcgctcaa ctgctcacag gtgatgattt
    25981 cgggcacgaa gtccgtgaag atgagcgagt cgctcacaaa tatggcattc acagcgtacc
    26041 gttttttgtg atcaatgaaa aactcggggt atccggggct caaccgccag aaatattact
    26101 tgacgctatc aaacaggcat tgcaaaaata atccgaaaaa agaccgcttg ttaagctaaa
    26161 caagcagtct tattcttctt ataatttaca atttattttt tggtgatcgc acctaaaata
    26221 ccacgcataa tttgtttggt gacttggttg cgtaaatttc tgccaactga atgcgccact
    26281 tggctaacca cttgttcggt aatcgattga tcttttttct tcttcgagcc aaaaatggaa
    26341 gagagaatac cgccaaagaa gtcattgttt gcttcttctg ccgcagcctg ctctgcttta
    26401 attgcttctg ctttcgcatt aagtaattca aacgcagatt cattatcgac atattggctg
    26461 tagtgatgat agagatcatc ttgcttcact aaactatcac gttcgcctgt ggctaatgga
    26521 gctaattggc tttttggtgg atagatatag gcaatttcta caggcgttgg catacctttt
    26581 tcatctagca ccgaaaccaa cgcttgcccc acaccaagtt gcgtgatggc ttctaccaca
    26641 tttacctctt tattcgctcg gaaagtttcc gctgctgatt ttaccgcttt ttgatcacgt
    26701 ggcgtgaaag cacgcaaagc gtgttggata cgattgccaa gctgccctaa tactgaatca
    26761 ggtatgtcca acggattttg cgtaacaaaa taaacaccca ccccttttga gcgaattaaa
    26821 cgaaccacct gttcaatttt atccaccaat gccttagggg catcgtcaaa caataaatga
    26881 gcttcatcaa agaataaaac aaacgtcggt ttttctgggt cgccaacttc tggcaaattc
    26941 tcaaagagct ctgccataaa ccaaagcagg aatgcactgt acactcttgg tgagttaatt
    27001 aatttttctg cattcaacat attgattaca ccacgcccat tacgggtttg gatccaatcg
    27061 tgcaaatcta atgcaggctc accaaataaa ttatctgccc cttcattttc aagccttaat
    27121 aacgcacgct gaatagctcc aacgcttgct gcagaaatat gcccatattc cagttggaat
    27181 tctttcgcat tatctgcgac aaatttaagc aacgcacgca aatcttttaa atcaattagc
    27241 aataaacctc tgtcatctgc cacacgaaaa acgagattta acaagcctcc ttgggtatcg
    27301 tttaaattga gtaaacgaga aagtaacatc ggtcccattt cggaaatagt gattcgaagt
    27361 gggatacctg atgtaccaaa tacgtcccaa aacgaaactg gatagccctt taagtaatct
    27421 tctcccccta aattaaactg taaaatgcgt tctgcaattt ttcctgaaaa attacccgct
    27481 tgtaccgagc ccgaaagatc accttttaca tctactaaaa atactggcac gccatcatca
    27541 ctcaaagatt ctgccaattt gcgtaaagta accgttttac ctgtacccgt agccccagca
    27601 attaaaccgt gccgatttgc cattttggca ttaagcgaaa taactttgcc tgtttctgtt
    27661 cgagcaattt gatacaaact catctttcat tctcttaaag gtaaattaat tttttgttct
    27721 catcatataa aaatttatca cttaattacg gctattcttt ttcgtttttg ccaagaacgt
    27781 ttttttactt cttgagcggt tcactttcgg taaagtcagt aaggattcct tgtcgtattg
    27841 actcacagga atctgatgcc caatatactc ttcaattgca ggcaaattaa ccgcataacg
    27901 ctcgcaagca aaactaattg aatggccgct ctcccctgct cgccccgttc taccaatacg
    27961 atgaacgtaa tcttctttat catcaggcag atcataattg aaaacgtgcg tgacatctgg
    28021 aatatgcaat ccacgagccg caacatctgt taccactaaa atatccaaat tgcctttggt
    28081 gaaattatcc agcaaagaaa ggcgtttttt ctgcggaata tcgccggtta gcattccaac
    28141 ccgatgtccg tctgccgata aatactgcca gatctcttcg catttatgct tggtgttggc
    28201 aaaaatcata caacgctccg gccactcttc ctcaagcaat gtcagcaaca atgccatttt
    28261 gtcttcattc gaggggtaaa atagctcttc tttaatacga tgtccggttt tttgtagtgg
    28321 ctcaatttca atatattccg cattatccat atcttcatag gcaagttccc gcacttttgc
    28381 cgaaagcgtt gccgaaaaga gcaaagttaa acgttctgaa ggcttaggtg cttttcgcat
    28441 taaataacgg atatctttaa taaaaccaag atcaaacatt cgatctgctt catctagcac
    28501 aatcacttga attttgtcta aattgataat gccttgtttt acatagtcaa tcactcgacc
    28561 tgttgtaccg acaagaatat ctaccccttt ttcaatttct tgaacttgtt tgtcgtaacc
    28621 atcaccaccg tatgccaaag ctagttttaa atcagaatgt tttaaaaaca gctcggcatc
    28681 tgccccaatt tgaaccgcca attcacgggt tggagcaaga attaatgctc tcggctgatt
    28741 ttttgacgtt tcgatttcgt tattcaataa atggttaaaa gttgcaatca aaaaggcaag
    28801 cgttttccct gtacctgttt gagcttgccc tgcaatatct ttgcctttca gcgtaatcgg
    28861 aaaggttttt gcctgaatcg gcgtacaaaa ttcaaagcct ttgctatcaa gggcatttag
    28921 tacattagcg tgaatcggga agtcaataaa gcggtgttcg gtaagatgat tagacatagt
    28981 atttccttga taattgtgat gtgacaagcg gtctttttta cacatttttt tgcaaaatag
    29041 catattgggc gttttttctc aaatttaacc gcttttgagt ttattcggca aaatagattg
    29101 gaaaagctct cggcttttca gcggttgtga ccctaaatag ggctttagtg ccattcgggt
    29161 aaaacgtttc gctgcttgtt gagtactttt ttctgaaaaa tccaactgag caaacgctaa
    29221 taaatcactg cctaaaaagc tctggttatt ctgtaataat gaggcgataa agccctgatt
    29281 ttcctgaaat tgataggtca ttttcggatc aactggcaaa ccggttgccg cacagtgcgt
    29341 aaaatccacc ccataaccca atgcttttaa cgtatgaaat tcaaaggtgc gtaaaatcgg
    29401 ctcaatctgt ttttgtgtgg caagctcggt aatgcaacgt aaataatgct gaaataattc
    29461 agggtaagcg gtctgatttt ccaatactct tgcaagtacc tcgttgatat aaaacccact
    29521 ataaagtgaa agcgtttcca tcggcaacgt taatgcagcc ggttctgctt tggttaaagt
    29581 tttcagatcc cctttacccg accaacgcaa caacaacggc gtgaacggct gtaaaaccgc
    29641 tttcaacggc gagcgagccc gccttgcacc tttagcaagt aaagtaatcc gcccgtgatg
    29701 ttcggtaaaa aactccacta ataaactgtt ttcgctgtat tcacgccgat gaaggacaaa
    29761 ggctcgttgc cattggtcta ttgtcataat ttcttgttaa ttatgaggtt gtaatttact
    29821 tctgccaaat ctccccctcc cctctttgct aaagagggga gatcttgcag agtatatttt
    29881 aaagtcattt tcactattta accttttata tagataggaa gatgaattta aaatttctgg
    29941 agaactagtc cccctcttta gaaaagaggg gttaggggag atttgattac gatattaatg
    30001 ctataacggg agccaactac tcgtccatat accctaaact tctcaatgct ctttcatcat
    30061 ccgcccaacc agcttttact ttcacccaaa gctcaaggtg cacttggttg tcaaataggc
    30121 gttccatatc ttttcgagct tcaatgccga tggttttgat tttttgcccc cccgcaccaa
    30181 taaccatttt tttctgacca tctcgttcta ctaaaatcaa gccattgatt tcatacaccc
    30241 cacgctcatt cattttaaat tgttcgattt ctaccgttac cgaataaggt aactcttcac
    30301 ctgtaaaacg cattagtttt tcacgaataa tttctgatgc cataaagcgt tgagagcggt
    30361 ctgtcacata ctcttcagga aagtggtgct caccttgacg cagtgattga cggacaaatt
    30421 tctgtaaaat atgcacattt ttaccccgtt gtgccgaaat tggcagaatt tcagcaaaat
    30481 caaatttttg tgaaagctcg gtaatatgcg gcagaagttc ttctttttcc ttgatgttat
    30541 caattttatt gattgccaat aaaacagggg cttttgccga acgcaatttg tttaacacca
    30601 tttcatcatc ttctgtccat tttgtgcctt ccacaacaaa gatgatgaga tctacatcgc
    30661 caatagcaga acttgctgca cggttcatta aacgattaat cgcacgtttt tcttctatgt
    30721 gtaagcctgg ggtatctaca taaatcgctt ggtattgatc ttcagtgtga ataccaacaa
    30781 tacgatggcg agtcgtttga gctttgcgtg aggtaatgga aattttttgt cctaaaatct
    30841 tatttaataa tgttgatttc cctacatttg ggcgaccaac aatcgcaata aaaccacaat
    30901 aggtttttgc aggagtttga atttgttctg tcattttgtt ttcctttcat tctgtggaat
    30961 gaatttgatg gtgcaagcgt ccacgcttgt accatttaat taaatcttta gcacaagcga
    31021 ggacgcttgc gctatcttac tttttcgttt taaccaccgc taacgctttt tctgcggcat
    31081 tttgttcggc ttttcggcgg ctggtgccaa tgccgataaa cacttcctcg acgttaatca
    31141 ctttacaggt tacacggaaa gtttggttat gagcttcgcc tttaatatct aatacatcat
    31201 attcaggcaa tggcaatttt ctaccttgta aaaactcttg caggcgggtt tttggatctt
    31261 tttgtgaatc gcctggactg atatctttaa gcaaatctga ataccaatct tccacctttt
    31321 ccattgcttt tgccatatct tgatcgagat aaatggccgc aataatcgct tcaacacagt
    31381 ccgataaaat cgattctcgg cgaaaaccac cactttttag ctctcctgga cctaatttta
    31441 ggtagtcgcc taaatcaaat ttgcgagcaa taatcgctag agtttgctca cgcacaagag
    31501 ttgctctcat acggctaagc tcaccttcat tcgcttttgg aaatttgtcg tataatactt
    31561 taccaatagt aaaatttaaa attgaatcgc ctagaaattc aagacgctca ttgtttttgg
    31621 ttgccgcact gcggtgtgtt aaggcctgaa tgaggtaatc aaggttatga aattgataac
    31681 ctaatttttt ctgtaatcgt tctagttgca tagtttatgt gaataaaggg gcgtatctca
    31741 tacgcccctg ttaagattaa ttgattgagg taaacattct agaaaaacga agccccgttg
    31801 ggaattcatt tgctttttta tctaaactta accaaataaa agtggcttta ccaacgatgt
    31861 ttctctcagg cacaaagccc cagaaacgac tgtcttcact gttatcacgg ttatcaccca
    31921 tcacaaaata gtatccttgt ggcacaaccc attcaccttc tgcttgccct tcttgttggt
    31981 agaaataagg tgcatagtta aatgccattg ggtgatttaa aatttgatga gttacattac
    32041 ctttttcagt gcgttctacc tgcatttcac cgtgatagaa aaactcagga tttggcttgc
    32101 cttcgctgta ttcaaatacc tttgtttcgc cattggcttt agtaaccgtt aattgctgag
    32161 ttgcaaaatc atatttaatt ttatcaccac ctactgccac cacacgctta atataatcaa
    32221 cgtgtggttg tgccggtgct ttgaatacaa tcacatcgcc tcgttctggt ttgcccgttt
    32281 caatcaaggt gttttgccag ataggatctt taatgccgta gctgaattta tttacgacta
    32341 aaaaatcgcc tacacgcaag gttggctcca ttgaaccact tggaatttga aacggttcaa
    32401 ataagaacga gcgaagtact gtaacaaaga ataaaatgcc gaaaagtgag gcaaaaaatt
    32461 cgcctactgc ggatttaggt tgaatttccg ctatttcttc agcggttaat tctctgccta
    32521 aacgtttttg ttgtagaaca atcgcctgtt tacgtcttgg gtcagcactg aatttgtaaa
    32581 acgcccaaaa tccaccacaa ataataacca aggcaactaa aataatggaa atagtatttg
    32641 gcagttgcat tgaatcaagc cattgccaaa taccataaat aatacctaga aaaataattg
    32701 gcataaattg tgccactgaa tgctcctttt tattcaaaat tgcaaagttt tctcaaaata
    32761 tgaccgcttg caagcggtcg tattttacac gaatattgac aattaatcct tacccacatg
    32821 aagaattgcc aaaaatgctt cttgtggcac ttctacgtta ccgagagatt tcattcgttt
    32881 tttaccctct ttttgttttt gtagcagttt cttcttacga cttacatcac caccgtaaca
    32941 ttttgctaat acgtttttac gcaactgttt taccgttgag cgagcaatga cgtggttacc
    33001 aattgccgct tgaatagcga tgtcaaattg ttggcgtgga ataagttcac gcattttttc
    33061 cactaattca cgtccacgat acgccgcatt tgcacggtga acgataatcg ccaacgcatc
    33121 aacacgctcg ccgttaatca taatatccac acgcaccata tcagccgttt ggaaacgttt
    33181 aaaaccgtaa tcgagtgaag cataaccacg agaggttgat tttaagcggt cgaagaagtc
    33241 taacactact tcgcccattg ggatttcgta agttaaggcg atttggttac cgtgatagac
    33301 catattggtc tgtacgccac gtttttccac acaaagggta atcacgttgc ctaggaattc
    33361 ttgtggcaca agcatattac attctgcaat cggctcacga atttctgcaa tattgttgat
    33421 tgctgggagt ttcgatgggc tatccacata gatcgtttcg ccactggttt gcaccacttc
    33481 gtaaactacg gtcggagcgg tagtaatcaa atcaagatcg tattcacgct ctaaacgctc
    33541 ttgaatgatc tccatatgca acaagcctaa gaagccacac cggaagccaa agcccaacgc
    33601 acttgaagtt tctggctcat agaacaatga cgcatcattt aggcttaatt tgcctaaagc
    33661 atcacggaag gcttcgtagt cgtctgaact aatcggaaat aaccctgcat aaacctgtgg
    33721 ttttactttc ttaaagcccg gtaatacttc agttgctggg ttgtggtggt gggttaatgt
    33781 atcgcccact ggtgccccta aaatatcttt aatcgcacaa actacccaac ctacttcacc
    33841 tgtttttagc tcggtagtat ccacttgttt tggcgtgaaa ataccaaggc gatctacatt
    33901 gtaagactgc cctgtactca tcactttaat tttatcgcct tttttcagca cgccattttt
    33961 cacacgcact aaagacacaa cgcctaaata gttatcgaac caagagtcga taattaacgc
    34021 ttgtaatggt gcttccgcat cgccttctgg tgcaggcatt ttcttcacga tttcttctaa
    34081 aacatcttca atgcctaaac ctgttttagc tgaacagcgt actgcatcaa tggcatcaat
    34141 gccgacaata tcttcaatct cttctgccac acgctcaggc tcggctgctg gtaaatcgat
    34201 tttatttaaa attggcacaa cctctaaatc tatctcaatt gcggtataac aattcgccaa
    34261 cgtctgtgct tccaccccct gccctgcatc aaccactaat aaagcacctt cacaagcagc
    34321 aagcgagcga gaaacttcat atgagaaatc cacgtgccct ggcgtatcaa taaagttgag
    34381 ctgataaatt tcgccatctt tcgctttata gtttaaggtt acactttgtg ctttaatagt
    34441 aattccacgc tcacgctcaa gatccattga atctaatact tgagcctcca tttcacgatc
    34501 tgataagcca ccacaagttt gaattaagcg gtctgataaa gtcgatttgc cgtgatcgat
    34561 atgagcgata atcgaaaagt tacgaatatt ttgcattttc atcgggcagt taatgtcctg
    34621 ttttgttatt tgtttatcaa aaattaatcg gggaattgta cttgaaatac aaggttttgc
    34681 aaagaaaaca aatagcaata agcggttatt ttttcctaaa aatgtgcaaa tttcttgaga
    34741 aaaatgaccg cttgcactca ccctaattgc aaataatttc tctaaaatat aaaaaacatt
    34801 gagcaatcac aaatttttat tatagaatcc ataagttttt ttaattcatt caattatagg
    34861 aaataattat gcaattcaga gaaattaatc cctcccctcg tttattaatg tgccctggcc
    34921 caacagatgc tgacccacgt gttttgcgtg caatgtctgc acccatttta gggcaatttg
    34981 accctgagtt tacctcatta atgaatgaaa caatggaaat ggaacgccgt ttattccaaa
    35041 ccaaaaatga gcaaacttat gtagtcaact ccacctctcg tggtggctta gaaactgttc
    35101 taaccgcagc gatttgccca ggcgataaag tgttaattcc tgcatttggt cgttttggtt
    35161 atttattaaa tgaaatttta gctcgtagcg gtgcagatat taccattatc gaaagagagt
    35221 ggggaaccgt atttgaacca gaagaaattg aagcagagtt gaaaaaaggc ggctataaag
    35281 cagttgctat tattcacggc gaaacctcaa cctctatgat gcaaccgtta gaagagatcg
    35341 gcaaaatctg taaacaatat gatgcaatgt taattgtaga taccgttgca accttaggcg
    35401 gtgtagatat tcgtgtggat gaatggggca ttgatgcttg tatcggcggt acacaaaaat
    35461 gtatttccgc cccatcaggc acagctttaa tcacttataa caaacggatt gaagagatta
    35521 ttgcaaaacg taaacgtgtg gaaaaaggca ttcgtgcaga gtctgatgta gatggcacat
    35581 taccgccaat tccaagtaac tacttagact tggcacagct acaagattac tggagcccac
    35641 gccgtttaaa tcaccacaca gaaaccactt ctgcacaata tggtgtacac gaagcgttac
    35701 gcattatttt acaagaaggc ttagaagctc gttttgctcg ccatatttta aacgacaaag
    35761 cattatgtgc aggtttagaa gcaatgggtt tgaaaatttt tggcgatcgc aaacataaag
    35821 cccccgttgt aacggcattc cacattccag aaggtatgag tggcccagca ttgcgtagtg
    35881 atattttaaa ccacttcggt attgaattag ccacctcatt cggcccctta gatggaaaag
    35941 taatccgtat cggtaatatg ggtttctcaa gccaaaaacg taacgtactt ttaaccttag
    36001 gtgcgttaga agccgtactt ttaagccata aagtaaaagt gccttcaggc gatgcggttg
    36061 cagctgcctt ggcagtctat aaagaggctg ataaataacc accttcctaa atttacaata
    36121 caactcacta ataattaata ctgtggatat ttatccacag taaaaccaca gaaactcaaa
    36181 atattttcac aaatttaatc ttttcctatt tcatttaatc aaattaaata ttatgacacg
    36241 gagcattttt ttaagaatga aaagtaaaag ttttaatgag gaaattttga tatgccaaac
    36301 tttagtttca cccatctgta taaaaaacac acaaaagagc tccctactgt taccttgctt
    36361 agcgctctga tagggcttag ccaagtctct tatgccaatc cacttccttt aggcaacacc
    36421 actggcacca ataaccttgc tgtaggtgaa ggtagttttg cgttaggcac aggttctcaa
    36481 gcaatcggca agaatgccat tgcaaccggt ggtaatatct ccccccagga attcaatgtg
    36541 gagttagaaa acttccgtga tttagttaat caaattgaag agttacgtac tcaaattgct
    36601 gaacaagaaa aacaaggctc tgttaatcaa caattacaag atgctttaaa gagtcagatt
    36661 gctcaatatg acactatttt agagcgtgta cgtgaaaaac aagcacaaca agcaatatta
    36721 gccaatagtg ttatatcaat gcggaaagaa ttagttaata gaagaaatca gttaacagaa
    36781 ttggaaaata attttaatac tagtttttta acaggaaaaa ataaacaa
//
LOCUS       DANLXS010000006        36585 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.006, whole genome shotgun sequence.
ACCESSION   DANLXS010000006 DANLXS010000000
VERSION     DANLXS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 36585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36585
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.006"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..982
                     /gene="pheS"
                     /locus_tag="PXD71_000237"
     CDS             <1..982
                     /gene="pheS"
                     /locus_tag="PXD71_000237"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL6290406.1"
                     /translation="HLKDLTNKAREALDKIDQNLEALEEFRVEYFGKKGHFTTLMQEL
                     RNIAAEERPAIGAKINEAKQVVLDVLNAKKEEWEQAVLNAKLANESIDVSLPGRKTEL
                     GGLHPVSITIERVVKFFSELGFTVANGPEIETDYYNFDALNIPDHHPARADHDTFWFD
                     ANRLLRTQTSGVQIRTMENVKPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKEAN
                     FTELKGLIHDFLKAFFEEDLQVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMVHP
                     NVLRNVGIDPEEYSGFAVGMGVERLTMLRYNVTDLRSFFENDLRFLKQFK"
     gene            1030..3417
                     /gene="pheT"
                     /locus_tag="PXD71_000238"
     CDS             1030..3417
                     /gene="pheT"
                     /locus_tag="PXD71_000238"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL6290407.1"
                     /translation="MKFNESWLREWVNPAISTEQLCDQITMLGLEVDDVEPVAGAFSG
                     VVVGEVVECAQHPDADKLRVTKVNVGGERLLDIVCGAPNCRQGLKVACAVEGAVLPGD
                     FKIKKTKLRGQPSEGMLCSYSELGIKEDHSGIIELPADAPIGQDFREYLNLNDVAIEI
                     SLTPNRADCLSIAGIAREVGVINRVDVKAPTITDVKATISDKVSVELQAPEACPRYLA
                     RVVKNVNVKATSPLWLQEKLRRCGIRSIDPIVDITNLSLLELGQPMHAFDAAKIEGGI
                     QVRMAKEGEELVLLDGTNAKLQSNTLVIADSKGALAMAGIFGGEASGVSENTKDVVLE
                     SAFFAPLAITGRARQYGLHTDASHRFERGVDPELTRQAMERATALLLEICGGEAGEIV
                     EAVSQENLPKRNTVTLRRSKLDSVIGHHIDDATVTDILTRLGLNVTFADNSWTAIAPS
                     WRFDIEIEEDLIEEIARIYGYNSIPNNSPLAHLTMKGTPEKLLEANRIRMALVDSDYQ
                     EVITYSFVDPKKQSLLHPQQEALILPNPISSEMSAMRVSLLTGLLDTIVYNQSRQQNR
                     VRIFEAGLRFIPDSNAESGVRQEFVFGAAIVGDKRPVHWENKSEAADFFDLKGDIERI
                     LSLTSIRHDLQFVAKQFPALHPGQSAAIMLDGKEIGFIGTVHPSIVQKFGIKGKPIVC
                     EILASAIAERPVTQAGEISKFPANNRDIAVVVDESVPAGEVLEACRKAGGNKLVAVNL
                     FDVYRGSNLESGKKSLAISLTVQDNEKTLEENEINAVIQAVLAELATRFNAVLRD"
     gene            3475..3771
                     /locus_tag="PXD71_000239"
     CDS             3475..3771
                     /locus_tag="PXD71_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDL6290408.1"
                     /translation="MALTKIEIAENLIEKFGLEKRVAKQFVELFFEEIRSSLENGEEV
                     KLSGFGNFSLREKKARPGRNPKTGENVAVSARRVVVFKAGQKLRERVEQASSQS"
     gene            3810..4331
                     /locus_tag="PXD71_000240"
     CDS             3810..4331
                     /locus_tag="PXD71_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6290409.1"
                     /translation="MKLTKRWLSLGCLLAITAMLVACSSSDTTSKSQVKSKVYRSIYQ
                     SDLSDPIMAISRLSEHQHEWKGTRYRLGGNSKAGIDCSGFMQVTFKDLFGIDLPRTTT
                     EQAKAGERISKDELRTGDLVFFKTGRGPNGKHVGVYVKNGQFLHASTKGGVIYSDLDS
                     PYWSKAFWQARRL"
     gene            4347..5390
                     /gene="degS"
                     /locus_tag="PXD71_000241"
     CDS             4347..5390
                     /gene="degS"
                     /locus_tag="PXD71_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDL6290410.1"
                     /translation="MLKKIGQAVIFGLFCAILILFVAPIIQSKGSSFSLSAFTQKEIV
                     SYNAAVKIASPAVVNVYSRNFSSENETFEVKNLGSGVIMSSDGYILTNRHVIEDADQI
                     VIALQNGAISTNVKLVGDDSLTDLAVLKIEGENLPTIPQNPERKLQIGDVVLAIGNPL
                     NLGQSITQGIVSAIGRKTLTESGRQNFIQTDVSINQGNSGGALINTAGELIGINTLRF
                     GKNTNELSEGLNFAIPINLANQIMSKIIKDGRVIRGYFGVSTDLLDTAKMQGYEKGVI
                     IVNVDKNGPAAKAGIQAGDLVLKVGDIEAFSPSQMMEELAEMKPYTTVKVLVQRDEQL
                     LNFDVTITELQTQ"
     gene            5399..6073
                     /gene="cmk"
                     /locus_tag="PXD71_000242"
     CDS             5399..6073
                     /gene="cmk"
                     /locus_tag="PXD71_000242"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL6290411.1"
                     /translation="MKKIVITVDGPSGAGKGTLCHALAEKLGFDFLDSGALYRITALS
                     AIKKEVPLDDEEKLAEVGRNLDIQFLPENGEIQVILEGENVGDLIRTAEAGQNASKVA
                     AFPLVREALLERQRAFSSEKGLIADGRDMGTVVFPDAQIKLFLDASAEERTKRRVKQL
                     QEKGFNANFDEILAEIKERDFRDRNRSVAPLVPAKDALLLDSTHLSIEDVINQALNHI
                     AQYIKF"
     gene            6165..7832
                     /gene="rpsA"
                     /locus_tag="PXD71_000243"
     CDS             6165..7832
                     /gene="rpsA"
                     /locus_tag="PXD71_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL6290412.1"
                     /translation="MSELSFAQLLEESFAATPRLGDVVKGTVVAIQKGLVIVDTGFKS
                     ESAIDASEFTNAQGELEVQVGDEVDVVLKAVEDGFGATVVSRGDAKRNEAWIALEKAF
                     EEQATVIGFVNGKVKGGFTVELNGVRAFLPGSLVDTRPVRDSLNLEGKEYEFKVIKLD
                     QKRNNVVVSRRAVIETESNQDRDAVLANLVEGSEVKGTVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEVVNVGDEVTVKVLKFDKDKTRVSLGLKQLGQDPWAVIAENHPV
                     NSKLTGKVTNLTDYGCFVEILDGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLE
                     VDEERRRISLGLKQCKANPWEQFAATHSKNDKVSGKIKSITDFGIFIGLEGGIDGLVH
                     LSDISWNVSGEEAVRNYKKGDEVEAVVLQVDAVKERISLGIKQLESDPFTNFVDGTKK
                     GAILTGKVVEVDAKGVKVELEGGVEAFIRANEATAERVEDITSVISVGDSIEAKYTGV
                     DRKSRVVNLSVRAKNEAEESAAIAQVNKEEVVVPNAFAEAFKAAKGE"
     gene            7961..8242
                     /locus_tag="PXD71_000244"
     CDS             7961..8242
                     /locus_tag="PXD71_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDL6290413.1"
                     /translation="MTKSELIESLASKNPSLPIKMVEHCVKELLEQLTATLEEGERIE
                     VRGFGSFSLHYRQPRLGRNPKTGESVLLGAKYVPHFKAGKDLKERVDLL"
     gene            8279..8566
                     /locus_tag="PXD71_000245"
     CDS             8279..8566
                     /locus_tag="PXD71_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL6290414.1"
                     /translation="MIKYILGILIVIAVIIVAVTIGANNDQLITFNYIFAQSELRLST
                     LVAILFGLGLILGWLVTGIFYLKIKFQNVALNRRVKRQAQQITELTTPKAE"
     gene            8570..9766
                     /gene="lapB"
                     /locus_tag="PXD71_000246"
     CDS             8570..9766
                     /gene="lapB"
                     /locus_tag="PXD71_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDL6290415.1"
                     /translation="MLELLFLLLPIAALYGWYMGQRSAKKDQETASNKFSREYVTGLN
                     FLLSNQQEKAVDLFLSMLQKQESENHIASESQFEAELTLGNLFRSRGEVDRALRIHQT
                     LVNSPHYSFDQKLLAKQQLAKDFMVAGFYDRAENYYISLVDEPEFAKYSLSQLAVIYQ
                     KTREWKKAINVTEKRLRIEPDADKIPLSHFYCEYAQGVKFDDKAAFIESLNKALEYYP
                     QSTRASIILGDFYLEEGEYLRALSYFEQILVQDPDYISEVLGRIKQSYMALEDLNGYE
                     LFLIRANRVKHNSSVDIALTEFIEEKDGINAAHSKLYQQLSTYPNLITFHRFIRYQAD
                     FAEEGNGKESLVLLHNMVGNQIKRSFQYRCLNCGYQSYRLMWQCPSCNQWEKIKPVQS
                     IEGIIQ"
     gene            9790..10482
                     /gene="pyrF"
                     /locus_tag="PXD71_000247"
     CDS             9790..10482
                     /gene="pyrF"
                     /locus_tag="PXD71_000247"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL6290416.1"
                     /translation="MNNKVIVALDYETEREALEFVDLVDPSLCRLKVGKEMFTTLGTN
                     FVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDLHASGGLAMMEEAKKIL
                     EPYGKEAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
                     VEVLRTHCGQDFKLVTPGIRPKGTDFGDQRRVMTPKQAVETGSDFLVIGRPITQAQDP
                     LAVLKSINQSIS"
     gene            10565..10876
                     /gene="yciH"
                     /locus_tag="PXD71_000248"
     CDS             10565..10876
                     /gene="yciH"
                     /locus_tag="PXD71_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDL6290417.1"
                     /translation="MTLVYSTETGRIKSEVIKEERPKGDGIVRIQRQTSGRKGKGVCL
                     ITGLDLEDKALNSLASELKRKLGCGGSFKDGVIEIQGDNRGFIKQILEQKGFKVKLAG
                     G"
     gene            10979..13141
                     /locus_tag="PXD71_000249"
     CDS             10979..13141
                     /locus_tag="PXD71_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDL6290418.1"
                     /translation="MWKKTLIAVFLASQYAFAVGTGKEPSKSYTETELKQLKNDWLAI
                     YQKELQYAENLRLKQRENFQQINYLADAAISQKNLSLSTEQIIQRLLLTLAGYPLEMK
                     AEWKLLNAKITLKQATESEVKAFISKYPDSLYQRQLQNLMFELLYREQKFPELLEYAQ
                     NVKQSSLADQCRLFSARYELAASKAQINPEVSQVTEQKAENAELLALVQEFDRFWLAT
                     PKLTSDCANLESYWRDQGLKTDEKMRLKAVELIKQNANAELANLAANVNNENLKIWLS
                     SVEKVANNPADLQNFIENQPLDSPFYADNKAIIQQLFAKYVRTLSENMENPSFQQYQT
                     WAEKYQLSSEEINGWKNAFITRFFDNPEPTFQFWRDEQIKLLKTDNLTERRLRMAIWQ
                     KADLNEWLVLLSQQAKEKAEWRYWAAKEEKNEAKRKVDFESLAKERGFYAMLAAQQLG
                     IVYQIPMLEVPELTQAQLELYANQLARIQELRELGQFDQARKIWIDWIKNIKPEAGEN
                     TPTKIQLALIKYAKDKNWYDLAVEGTIQTKAFDYLDLRLPNAYSDWFDLHLQDKKISK
                     TFAQAIARQESAWNAQAQSHANARGLMQLLPTTAQKTAKDNGLTFKDGNDLFQPFNNI
                     MLGTTHLMELNEKYPNNRILIASAYNAGAGRVEQWLKRSNGQLAMDEFIASIPFYETR
                     GYVQNVLAYDYYYQMLYSDINDKNGLKMFYQEELTRKY"
     gene            13190..13266
                     /locus_tag="PXD71_000250"
     tRNA            13190..13266
                     /locus_tag="PXD71_000250"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13224..13226,aa:Met,seq:cat)
     gene            13493..13954
                     /gene="rimP"
                     /locus_tag="PXD71_000251"
     CDS             13493..13954
                     /gene="rimP"
                     /locus_tag="PXD71_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL6290419.1"
                     /translation="MATLEQKLEDLVLDSIEALGFELVGIECQRAGRFLTVRLYIDKE
                     GGVTIDDCSDVSRQVSAIFDVEDPIADKYNLEVSSPGLDRPLFTVAHYERFIGQDVVI
                     HLRIPMFDRRKWQGKIKQVEGDFITLTVENEDRQFAFGNIQKANLVPVFNF"
     gene            13975..15471
                     /gene="nusA"
                     /locus_tag="PXD71_000252"
     CDS             13975..15471
                     /gene="nusA"
                     /locus_tag="PXD71_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL6290420.1"
                     /translation="MSKEILLAAEAVSNEKQLPKEAIFEALETALTIATKKSRDLDID
                     VRVVVDRKTGDFQTFRRWAVVENVNNMTREISLEAAQFEDPNVQLGDFIEDEVESIEF
                     GRVAIQTFGQVVKTKIREAEKTKIIDEFRSDLGKIILATVKKVTRDNIFLELQGKSED
                     IKGEAVIVREDMIPRENFRPGDRVRGVLYEIKPESKGPQLFVTRAKPVMLEELFKLEV
                     PEIGEGVIEIKGSARDVGSRAKIAVKTHDKRIDPVGACVGMRGARVQAISNELGGERV
                     DIVLWDENPAQFVINAMAPADVSSIVINDETHSMDIAVEAKNLAQAIGRNGQNVRLAT
                     QLTGWALNVMTVEELTKKSEEEDGKVINLLTSALEIDEEFAQVLIEEGFTSLEAIAYV
                     PVDTLTAIDGLEDEDLVEELQSRAKNAITKKALEEEKLLKSAQIEEKLLTLEGMDRHI
                     AFKLAEKQITTLEELAEQGVDDLADIEELSEEKAGELIMAARQICWFN"
     gene            15456..18023
                     /gene="infB"
                     /locus_tag="PXD71_000253"
     CDS             15456..18023
                     /gene="infB"
                     /locus_tag="PXD71_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL6290421.1"
                     /translation="MLVQLSKEDNKMTEEKKTLSLGGARKASKVNTTTTSGKVKAVEV
                     KEKKPKIDAKALQEKAAKEAAAAKARAEKEAAEKLAKEKAEAEAKEKAAKKAAESTAS
                     KPISVPVMPSSKAKEEKPKAQQVQPKQEKAVDPEKEAKRKEEAELRRKQEELAQQKAE
                     MEAKRAAENARRLAEIAREEAEDNGNEDFDDERFTSSYAREAERDNDRRSEGNRGRNG
                     KGNVAKAKKGGREEDKNERSADRRNHKEVKGKGKQGKKGAALQQAFTKPAQVVKADVV
                     IGETITVAELANKMAVKATEIIKMMMKMGEMVTINQVIDQETAQLVAEELGHKVILRK
                     ENELEEAVLEDRDTHAEKVTRAPVVTIMGHVDHGKTSLLDYIRKAKVAAGEAGGITQH
                     IGAYHVETDDGKMITFLDTPGHAAFTSMRARGAKATDIVVLVVAADDGVMPQTIEAIQ
                     HAKAAGAPLVVAVNKIDKPEANPDRVEQELLQHEVISEKFGGDVQFVPVSAKKGMGID
                     DLLEAIILQSEVLELTAVKDGMASGVVIESYLDKGRGPVATILVQSGTLNKGDIVLCG
                     FEYGRVRAMRDENGKDVESAGPSIPVEVLGLSGVPAAGDEATVVRDEKKAREVALYRQ
                     GKFREVKLARQQKAKLENMFSNMAAGDVAELNIIVKADVQGSVEAISQSLMELSTDEV
                     KVKIVGSGVGGITETDATLAAASNAIMVGFNVRADASARRVIENENIDLRYYSIIYEL
                     LNDVKAAMSGMLQPEFKQEIIGLAEVRDVFRHPKFGAIAGCMVTEGIVKRNNPIRVLR
                     DNVVIFEGELESLRRFKDDVSEVRNGMECGIGVKNYNDVKVGDQIEVFEVVEVKRTI"
     gene            18108..18488
                     /gene="rbfA"
                     /locus_tag="PXD71_000254"
     CDS             18108..18488
                     /gene="rbfA"
                     /locus_tag="PXD71_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL6290422.1"
                     /translation="MSREFKRSDRVAQELQKEVAVILQREVKDPRIGMVTVSDVEVSR
                     DLAYAKIFVTFLFDNDAEAIKQGMQGLEKASPYIRTLLGKAMRLRIVPELRFIYDESL
                     VEGMRMSNLVSSVIKNDEEKHKED"
     gene            18491..19414
                     /gene="truB"
                     /locus_tag="PXD71_000255"
     CDS             18491..19414
                     /gene="truB"
                     /locus_tag="PXD71_000255"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL6290423.1"
                     /translation="MSRPRKRGRDIHGVFLLDKPQGMSSNDILQKVKRIFQANKAGHT
                     GALDPLATGMLPICLGEATKFSQFLLDSDKRYLVTAKLGERTDTSDAEGQVVETKAVA
                     VTKSQILAALEKFRGDILQVPTMFSALKHNGKPLYEYARAGITVEREARPITIFELNF
                     IEYKAPFLTLEVHCSKGTYIRTLVDDLGEELGCGAHVTMLRRTAVADYPIEKMMSYAD
                     LQNLAENQPLAELDQHLLPMDTAVSALAKINLSSEQTKAVGFGQRIRFDNLNQLYGLV
                     RLYSEDEQFLGVAEITNDTVIRPNRMINLTE"
     gene            19417..20497
                     /locus_tag="PXD71_000256"
                     /pseudo
     CDS             19417..20497
                     /locus_tag="PXD71_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
     gene            complement(20535..21059)
                     /gene="tadA"
                     /locus_tag="PXD71_000257"
     CDS             complement(20535..21059)
                     /gene="tadA"
                     /locus_tag="PXD71_000257"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL6290424.1"
                     /translation="MFIKPNQTSCPLQISEQDIAFMQYALDLADLAEAKGEIPVGAVL
                     VDKNQQIIGRGWNQTIQLCDPSAHAEMQAIRQAGQTLGNYRLLDCTLYVTLEPCPMCA
                     GAILHSRIKRLVFGASDYKTGAVGSRYHLFEDYKMNHFLEVHGNVLGRECSQKISDFF
                     KQRRLQKKGDALIK"
     gene            complement(21141..22949)
                     /locus_tag="PXD71_000258"
     CDS             complement(21141..22949)
                     /locus_tag="PXD71_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL6290425.1"
                     /translation="MTTESLTFADLGLPQPILDAVNEMGFVNPSPIQQECIPHLLAGR
                     DVLGMAQTGSGKTAAFSLPLLAQIDPTQRHPQMLVMAPTRELAIQVADACEQFAKNMK
                     GIRVVTVYGGQRYDIQLRALKQGSQVVVGTPGRILDHIRRGTLDLSELKFIVLDEADE
                     MLRMGFIDDVETVMAELPETHQTALFSATMPEPIRRITRRFMKDPQEVKIKATERSAP
                     DIEQSYWLVNGFRKNDALLRFLEVEEFDAAIIFTRTKTGTIDITELCERNGYRTAALN
                     GDMTQQAREQTLDKLRSGRLDILVATDVAARGIDIERISLVINYDIPLEEESYVHRIG
                     RTGRAGRSGRALLFVEPRERRLLRNIENLIKKPINEVAIPNHEILMEKRREKFKARIS
                     KQLEHHDLEKYRELLEDLFTADQDHEELAAAMMMLLQEKQKLILPPDPVVRAARSERG
                     RDRNERNDRRNGREHRENNGVAMDLYRIELGREDGVEVRHIVGAIANEGDISSRYIGH
                     IKLYDKYSTIELPQGMPNHLVQHFAHKARVLSKPMQMTLLGSANSASNANPFEGRGRR
                     GNDRNDRGGRRDGGFNKDRKGGFKEKRFSEKRGRRD"
     gene            complement(23151..24074)
                     /gene="nlpI"
                     /locus_tag="PXD71_000259"
     CDS             complement(23151..24074)
                     /gene="nlpI"
                     /locus_tag="PXD71_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDL6290426.1"
                     /translation="MVQLFKLFRIRFLLLNLISVLMLSGCTSRYADLVSIKNLALAEL
                     NPQLRFEQEAMVVRLSQVLEEAPLTPSEQADLYFERGVIYDSLGLWSLARYDFAQAIS
                     LNQRMAAAYNYMGLYLLLEGDYDSSIDAFNAVLELDREYSYTYLNRGLAFYYSGRYSE
                     AQRDLLHFYEEDKKDPYRALWLYFNELEFTPTEAKNNLQKRANNLSSDYWGTNIVNYF
                     LGKLNLAQVRAIMERDAKENSSQYAEILTETYFYLAKQQLKLDHKDEAISLFRLALAN
                     QVFNFVEYRFALFELSQIRGMTQTTPVVDKE"
     gene            complement(24155..26305)
                     /gene="pnp"
                     /locus_tag="PXD71_000260"
     CDS             complement(24155..26305)
                     /gene="pnp"
                     /locus_tag="PXD71_000260"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL6290427.1"
                     /translation="MTPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVV
                     AKKDVKEGQDFFPLTVDYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPVRPLF
                     PEGFFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQF
                     VLNPTTSEQKISRLDLVVSGTDKAVLMVESEADILTEEQMLAAVVFGHEQQQVVIENI
                     KEFVKEAGKPRWDWVAPEPNTDLINKVKALAETRLGDAYRIVEKQVRYEQIDAIKAEV
                     IAQLTAEDETVSEGTIIDIITALESQIVRSRIIAGEPRIDGRTVDTVRALDICTSVLP
                     RTHGSALFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRI
                     GSPKRREIGHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALM
                     DAGVPIKAAVAGIAMGLVKEDEKFVVLSDILGDEDHLGDMDFKVAGTRTGVTALQMDI
                     KIEGITPEIMRIALNQAKGARMHILGVMEQAIPAPRADISDYAPRIHTMKIDPKKIKD
                     VIGKGGATIRALTEETNTSIDIDDDGTVKIAATDGNAAKAVMARIEEIVAEVEVNQIY
                     NGKVTRVVDFGAFVSILGGKEGLVHISQITNERVERVADYLTVGQEVQVKVVEIDRQG
                     RIRLTMKDINNTNEANAEETVAENVVETEQENNF"
     gene            complement(26567..27847)
                     /gene="phoR"
                     /locus_tag="PXD71_000261"
     CDS             complement(26567..27847)
                     /gene="phoR"
                     /locus_tag="PXD71_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDL6290428.1"
                     /translation="MKVKLSLKHFLLELLLAAAVAGVFNLFFPAYFFWFSFCLLVLLI
                     WHHHTESSLLQLLRPNEQSGRSKVTTMEHLSQSAAFYHERLRQDKIKTLRLLSKLNKH
                     IQYLPDAIIICQENGEISWCNNVAQELFEFYWDKKSNKSIFNVIFYPEFKSYFRQQRH
                     HRPLVLLTHNKRYIELNLNRYDSNSRLIVARDVTSFVRLMHSRQTFLANMNHELRTPL
                     TILQGYLEMLENEPHNMALQQKALNAMKEQSQRMTNLLRQLSLLVKIEHSNDSEHNEV
                     QMSALISAIQKNTEILSQNQQILFDIEPNLTVWGDQEQLQSAVSNLIYNAIRHAGEGV
                     TIQICCQRDNQEPNTAIFSVQDDGIGIDAHHITHLTERFYRVDESRSNKTGGSGLGLA
                     IVKHALEQHHSTLEIESEAGKGSRFSFKLPLIKK"
     gene            complement(27850..28536)
                     /gene="phoB"
                     /locus_tag="PXD71_000262"
     CDS             complement(27850..28536)
                     /gene="phoB"
                     /locus_tag="PXD71_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HDL6290429.1"
                     /translation="MNNKILIVEDERAIREMLSLYLSKQYQVIEAEDYQSAINKLGEK
                     PELILLDWMMPGRSGIHLIKYLKKQPETEKIPIIMLTARSSEEDCITGLNAGADDYIT
                     KPFSPQVLLARIEAVWRRSYDPPSNLINIDGLILDQQAQRVLVAEQEVKLSGTEYKLL
                     HFLMTHPEKVYRREQLLDFIWGNDIYVEDRTVDSYIRRLRKSLEPYGFERYIQTVRGS
                     GYRFSNHFQD"
     gene            complement(28620..29390)
                     /gene="pstB"
                     /locus_tag="PXD71_000263"
     CDS             complement(28620..29390)
                     /gene="pstB"
                     /locus_tag="PXD71_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDL6290430.1"
                     /translation="MLPTDLETKLHINNLNFYYGDFHALKNINMRIAKNKVTAFIGPS
                     GCGKSTLLRTLNRMFELYPNQRATGEINLDGENLLTSDTDISIIRAKVGMVFQKPTPF
                     PMSIYDNVAFGIRLFEKLPKSELNDRVEWALTKAALWNEVKHKLNQNGDSLSGGQQQR
                     LCIARGIAVKPEVLLLDEPCSALDPISTMKIEELINELKHDYTVAIVTHNMQQAARCS
                     DYTAYMYLGELIEFGDTKQIFDKPKFRQTEDYIKGKMG"
     gene            complement(29400..30248)
                     /gene="pstA"
                     /locus_tag="PXD71_000264"
     CDS             complement(29400..30248)
                     /gene="pstA"
                     /locus_tag="PXD71_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL6290431.1"
                     /translation="MKNYQNRRFYCRKIKNVLMLSLAYSAVLFGLFWLVWIIVTLLQK
                     GIPALSWELFTRPTPAPNETGGLRNAIVGSILIVSTATLIATPIGILAGTYLSEYGRY
                     TKLAKFTRFLNDVLLSAPSIIIGLFIYAIYVAQVKHYSGWAGSLALAVLVIPIVVRTT
                     DSMLNLVPNNLREAAAALGCPRWRVITMISYRAARSGIITGVLLAVARISGETAPLLF
                     TALSNQFASLDMNAPMANLPVVIYQYAASPFADWNTLAWAGAMLITLFVLSLNIITRL
                     YFSKTK"
     gene            complement(30245..31204)
                     /gene="pstC"
                     /locus_tag="PXD71_000265"
     CDS             complement(30245..31204)
                     /gene="pstC"
                     /locus_tag="PXD71_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL6290432.1"
                     /translation="MHKHKIDNKCLNHPWVEGLFHHTTRFFALLVFALLVVVLVSLLL
                     GSWEALSRFGVSFLWENEWNPVNDRYGALVPIIGTLISAGIALVIAVPVSFGIATFLT
                     ELAPQWLKRPVGTAVEMLAAIPSIIYGMWGLFVFVPLFQEYVQPYLIDTFGQLPFIGM
                     LFSGAPFGIGLFSAGLVLAIMIIPFIASIMRDVFSVVPPLLKESAYGLGSTTWEVIWK
                     VVLPYTKTGVIGAIMLGLGRALGETMAVTFVIGNAFMLPDSVFSPSTSIASAIANEFN
                     EAGGLQKSALMSLGLILFLITSIVLAASRLLIARMGKQEGKRE"
     gene            complement(31285..32322)
                     /gene="pstS"
                     /locus_tag="PXD71_000266"
     CDS             complement(31285..32322)
                     /gene="pstS"
                     /locus_tag="PXD71_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL6290433.1"
                     /translation="MAKRFKLLTVLGISFSLSVAANAQTITGAGASFPYPIYAKWAGL
                     YEKETGNKINYQSIGSGGGQQQIIAKTIDFGASDDPMKGETLAEHKLLQFPAIIGGTV
                     PVVNIPELKSGELKLSGKVLADIFLGKITQWNDPAIVALNQDIALPAKTILVVRRSDG
                     SGTTFGWTNYLSKVSAEWKEKVGEGKSVKWPVGQGGKGNEGVAAYVKQLKYSIGYVEY
                     AYAKQNALAWVSMQNADGKFVQPSRETFAAAAANADWSQTAGMGVILTNEAGEKAWPI
                     TAASFILIHQHSDKPEQTKGVFKFFDWAFKHGKDAAAELDYVPLPDNVVEKIQTQWKT
                     EVKDGQGNSLY"
     gene            32507..34753
                     /gene="parC"
                     /locus_tag="PXD71_000267"
     CDS             32507..34753
                     /gene="parC"
                     /locus_tag="PXD71_000267"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL6290434.1"
                     /translation="MTTEINYEGIEQMPIKRFTEDAYLNYSMYVIMDRALPFIGDGLK
                     PVQRRIIYAMSELGLNASAKYKKSARTVGDVLGKFHPHGDSACYKAMVLMAQPFSYRY
                     PLVDGQGNWGAPDDPKSFAAMRYTESKLSKIAEILLGELGQGTVDYQPNFDGSLEEPK
                     YLPARLPHILLNGTMGIAVGMATDIPPHNINELADASVMLLDNPKATLDDVLSVVQGP
                     DYPTEAEIITPKAEIARMYEQGRGSIKMRAVWKKEEGEIVISALPHQASPSKIIEQVA
                     TQMRNKKLPMVDDIRDESDHENPIRIVIVPRSNRIDFDALMDHLFATTDLEKSYRVNM
                     NMIGLDGKPAVKNLLTILNEWLSFRRTTVTRRLNYRLDKILNRLHILDGLMIAFLNID
                     EVIEIIRNEDEPKAELMARFNLTDVQAEAILNLRLRHLAKLEEHQLQAEKSELEKERD
                     ELQLILGSERRLNSLIKKEIQADAKAFASPRRSPLVERAESKAIAESDLTPTEDVTVI
                     LSEKGWVRCAKGHDIDVQALSHRAGDGYLAHARGRSNQPVVFLDSTGRAYALDPTSLP
                     SARSQGEPLTGKITLPEGAVVQQVLMASPETKVLMASDSGYGFICTFADLVSRNKAGK
                     AVISLTENAKVLPPQLLENDENLSLVAMSNVGRMLVFPVSELPQLSKGKGNKIINISA
                     AAAKSGDEYLARLLVIKPTNSLVFVSGKRKITLKPSDVDNYRGERARKGSQLVRGLST
                     NSTVEIVE"
     gene            complement(34824..36176)
                     /gene="lysC"
                     /locus_tag="PXD71_000268"
     CDS             complement(34824..36176)
                     /gene="lysC"
                     /locus_tag="PXD71_000268"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDL6290435.1"
                     /translation="MSHLSVAKFGGTSVANFDAMTSSANIVIADANTRVVVLSASAGV
                     TNYLVELANGCEKEHRDEVIAAVRQIQYNIIEKLQHQDAVKAEIDELLERIETLAESA
                     SLATSAALSDELISHGEMMSTKIFTQLLIERGHPAVWVDVRDVVATNSHYGKAAPNDE
                     KTQQQSDNVIKPLIASGKIVITQGFIGRDDEGKTTTLGRGGSDYSAALLAEVLNANDV
                     LIWTDVPGIYTTDPRVVPNAQRIDTMAFNEAAEMATFGAKVLHPATLLPAVRSNIPVY
                     VGSSKAPEQGGTWVTRDPQPRPTFRAIALRRNQILLTLSSLSMLHAQGFLANVFAILA
                     KHKISVDVITTSEVSVALTLDKTGSASSGADMLSAELLDELNAYCNVQVESDLALVAI
                     IGNNLHAQSGVAKKLFHTLENYNIRLISYGASSNNVCTLVKNAEADDVVRSLHKGLFE
                     "
BASE COUNT        11076 a         6890 c         8220 g        10399 t
ORIGIN      
        1 acacctaaaa gatttaacta ataaagcacg tgaagcttta gataagattg atcaaaattt
       61 agaggcttta gaagagttta gagtcgaata ttttggtaaa aaagggcatt ttacaacgtt
      121 aatgcaagaa ttacgcaata ttgcagcgga agaaagaccg gcaatcggag caaaaattaa
      181 tgaagcaaag caagttgtgc ttgatgtgtt aaatgccaaa aaagaagaat gggaacaagc
      241 tgtattaaat gcaaaattag ctaatgagag cattgatgtt tcactgccgg ggcgtaaaac
      301 tgaattaggt ggtttgcacc ctgtttctat cactattgag cgtgtggtga aatttttctc
      361 tgaattaggt tttaccgtag caaacggacc tgaaattgaa accgattact ataacttcga
      421 tgcgttaaac attccggatc atcatccggc tcgagcggat cacgatacgt tttggtttga
      481 tgccaatcgt ttattaagaa cccaaacctc gggtgtgcaa atccgtacaa tggaaaatgt
      541 aaaaccgcct atccgtattg tggcaccggg gcgtgtatat cgtaatgact acgaccaaac
      601 ccacacaccg atgtttcacc aaatcgaatt gttatatgtg gataaagaag caaactttac
      661 cgagttaaaa ggcttaattc acgattttct caaagcattc tttgaagaag atttacaagt
      721 gcgtttccgc ccgtcattct tcccattcac tgagccttct gcagaagtag atgtaatgcg
      781 tcaaaacggc aaatggttag aagtgttagg ttgcggtatg gtacacccga atgtgttacg
      841 caatgtaggg attgaccctg aagaatatag cggttttgct gtgggaatgg gcgtcgagcg
      901 tttaacgatg ttacgctaca atgtcacgga tttacgttca ttctttgaaa atgacttacg
      961 tttcttaaaa caatttaagt aatacaagcg gttaaaaatt tagaaaattt tacaaatagg
     1021 atagaaataa tgaaattcaa tgaatcttgg ttgcgtgaat gggtaaaccc agcaattagc
     1081 acagaacagt tatgcgacca aattacaatg ttagggttag aagttgacga tgtagagccg
     1141 gtagctggtg cgttttcggg tgtagttgtg ggtgaagtgg tggaatgcgc ccagcacccg
     1201 gatgctgaca aattgcgtgt cactaaagtg aatgttggtg gcgaacgttt attagatatt
     1261 gtttgtggtg caccaaactg ccgtcaaggt ttaaaagtgg cttgcgctgt tgaaggtgca
     1321 gtgctaccgg gcgatttcaa aattaagaaa accaaattgc gtggtcagcc ttcagagggg
     1381 atgttatgtt cttactcaga acttgggatt aaagaagatc atagcggtat tattgaatta
     1441 cctgctgatg cacctattgg tcaagatttc cgtgaatatt taaatttaaa tgatgtggcg
     1501 attgaaatca gccttacgcc aaaccgtgcc gactgcttaa gcattgccgg cattgctcgt
     1561 gaagtggggg tgattaaccg tgtcgatgtg aaagctccaa ctattacaga tgtaaaagcc
     1621 acgatttcag ataaagtttc agtggaatta caagcaccgg aggcttgccc tcgctattta
     1681 gcccgtgtgg tgaaaaatgt gaatgtcaaa gcgacatcgc cactttggtt acaagaaaaa
     1741 ttacgccgtt gtgggattcg ctcaatcgat ccaatcgttg atattactaa cttgagcctt
     1801 ttagaattag ggcagccaat gcacgccttt gatgcagcga aaattgaagg tggcattcaa
     1861 gtgcgtatgg caaaagaagg cgaagaattg gtattgctag atggcacaaa cgctaaatta
     1921 caatcgaata ccttagtgat tgctgatagc aaaggggcat tggctatggc aggtattttc
     1981 ggtggcgaag ccagcggggt gagcgagaat acgaaagatg tggtgttaga gtctgcattt
     2041 tttgctccat tagcgattac cggtcgcgct cgtcaatatg ggttacatac cgacgcatca
     2101 caccgttttg agcgtggggt tgatccagaa ttaactcgtc aagcaatgga gcgtgcaacg
     2161 gcattattac tggaaatttg cggcggtgaa gcaggtgaaa ttgtggaagc cgtgagccaa
     2221 gaaaacctac caaaacgcaa tactgtcacg cttagacgca gtaaattaga ttcggtgatt
     2281 ggtcatcata ttgacgatgc aacggtaacc gatattttaa cccgcttagg cttgaacgta
     2341 acatttgcgg ataatagttg gacggcaatt gctccaagtt ggcgttttga tattgaaatt
     2401 gaagaagatt taattgaaga aatcgcgcga atttatggct acaacagtat cccaaataat
     2461 tcgccacttg ctcatttaac gatgaaaggt acgccggaaa aattactgga agcaaaccgt
     2521 attcgtatgg cattggtaga tagtgattac caagaagtga ttacttatag ctttgttgat
     2581 ccgaaaaaac aatcattatt gcacccacag caagaagcct tgattttacc aaacccgatt
     2641 tcaagtgaaa tgtcggcaat gcgtgtttct cttttaaccg gtttgttgga cactatcgtg
     2701 tataaccaaa gccgtcagca aaaccgtgtg cgtatttttg aagcaggtct acgttttatt
     2761 ccggatagca atgcggaatc aggcgtgcgt caagaatttg tttttggtgc ggcgattgta
     2821 ggcgataaac gtccggtaca ttgggaaaac aaatcagaag cagccgattt ctttgattta
     2881 aaaggcgata ttgagcgtat tctttcttta acctctatcc gccacgatct gcaatttgtc
     2941 gcaaaacagt tcccagcatt acacccgggg caatcggctg cgattatgct tgatggcaaa
     3001 gaaattggtt ttatcggaac tgttcacccg tcaattgtgc aaaagtttgg cattaaaggc
     3061 aaaccgattg tatgtgaaat tctagcatca gctattgcag agcgtccggt tacacaagcg
     3121 ggcgaaattt ctaaatttcc tgcaaataac cgtgatattg cggtagtggt tgatgaatct
     3181 gttcctgcag gtgaggtgtt agaggcttgt cgtaaagctg gtggtaataa attagtggca
     3241 gtaaatttat ttgacgttta ccgtggttcg aatttagaaa gtggtaagaa gagtttagcc
     3301 attagcttaa cggttcaaga taatgaaaaa acattagaag aaaatgaaat caatgcggta
     3361 attcaagcgg tattagctga attagcaaca cgtttcaatg cggtacttcg tgattaatca
     3421 gtaaaatatg gctgatttat tttatcagcc ataacaataa actaaggata aatgatggcg
     3481 cttaccaaga ttgaaattgc agaaaacctg attgaaaaat ttgggttaga aaagcgtgta
     3541 gcgaagcaat ttgttgaatt gttctttgaa gagatccgat cctccctcga aaatggtgaa
     3601 gaagtgaaat tatctggttt tggtaatttt tcactgagag aaaaaaaagc tcgtccagga
     3661 agaaatccta aaacggggga aaatgtggct gtttcagccc gtcgagtagt agtttttaaa
     3721 gctgggcaaa aattacgaga acgtgttgag caagctagct cccaatcata ggggcagtta
     3781 tattagttag atttaatgtg gagtaataaa tgaaattaac caaacggtgg ctttccttag
     3841 gttgtttgtt agctattaca gcgatgttag tggcttgttc aagttcagat acaacctcta
     3901 agtctcaagt aaaaagtaaa gtttatcgtt caatatacca atcagacctt tctgatccta
     3961 ttatggcaat tagtcgatta agtgaacatc aacacgaatg gaaaggcact cgctatcgtt
     4021 taggtggcaa tagcaaggct ggcatcgact gttctggttt tatgcaagtc acttttaaag
     4081 atctttttgg gatagatttg ccgagaacca ccacagagca ggctaaggct ggtgagcgta
     4141 tatccaaaga cgaattgcgt acaggtgatt tagttttttt caagacaggg cgtggtccaa
     4201 atgggaaaca cgttggtgtt tatgtaaaaa atgggcaatt tcttcacgcc tcaacaaagg
     4261 gaggggtgat ttattctgat ttggattcgc catattggtc taaggctttc tggcaggctc
     4321 gtcgtttata atttagggta acatacatgc tgaaaaaaat cggacaagcg gtcatatttg
     4381 gacttttttg tgcaatttta attttgtttg tagctccaat tattcagagt aaagggagtt
     4441 cgttttcatt atcagcattc actcaaaaag agattgtaag ctacaatgca gcggttaaaa
     4501 tagcctcccc cgcagtcgtt aacgtatata gccgaaattt tagttctgaa aatgaaacat
     4561 ttgaagttaa gaatttaggt tcgggtgtga ttatgagtag cgatggctat attttaacta
     4621 acaggcatgt gattgaagat gcggatcaaa tcgtgattgc tttgcaaaac ggtgctattt
     4681 cgactaatgt aaaattagtc ggcgatgatt ctttaactga tttagctgtt ttaaaaattg
     4741 agggtgagaa tttacctact attccacaaa atccggagcg caaactacaa attggcgatg
     4801 tggtgttggc aattggtaat cctttgaatt taggacagag cattacgcaa gggatagtca
     4861 gtgctattgg gcgaaaaacc ttaacagaaa gtggtagaca aaattttatt cagacagatg
     4921 tttccattaa tcaagggaac tcaggtggtg ctttaattaa tactgctggt gagctaatag
     4981 gtattaacac tttgcgtttt gggaaaaata cgaatgaatt gtcagagggc ctaaattttg
     5041 cgattcccat taatctggcg aatcaaatta tgagtaagat cattaaagat ggccgagtta
     5101 ttcgtggcta ttttggggta agtacagatt tgcttgacac cgcaaaaatg caaggttatg
     5161 aaaaaggggt gatcattgtt aatgtagata aaaatgggcc agccgcaaaa gcggggattc
     5221 aagcaggtga cttagtgctt aaagtcggag atattgaggc attctctcct tctcaaatga
     5281 tggaagagct tgctgaaatg aaaccttata ctacagtaaa agtattggtt caacgtgatg
     5341 aacaattact taactttgat gtaactatta ctgagttaca gactcaatag ggtagaaaat
     5401 gaaaaaaata gtgattacag ttgatgggcc gagtggtgca ggtaaaggaa cgttatgtca
     5461 tgcattggct gaaaaattag ggtttgattt tttagattct ggagccttat accggattac
     5521 tgcattaagc gcaattaaaa aagaggttcc ccttgatgat gaggaaaaac tagcggaagt
     5581 tgggcgtaat cttgatattc aatttttgcc agaaaatggc gagattcagg tgattttgga
     5641 aggagaaaat gtaggcgact taatccgaac ggcagaagca ggtcaaaatg cctctaaagt
     5701 cgctgctttc cctttggtac gtgaagcact tttagaacgc caaagagcct ttagtagcga
     5761 aaaaggtctg attgcagatg gacgagatat ggggacagtt gtgtttcctg atgcacaaat
     5821 taagctgttt ttagatgcaa gtgccgaaga acgcacaaaa agacgtgtaa aacagttgca
     5881 agaaaaggga tttaatgcta actttgacga gattttagcc gagataaaag aacgtgattt
     5941 tcgcgataga aatcgatcag tcgcgccact tgttccggct aaagatgcgt tgctgttaga
     6001 ttcaacgcat ttatcaattg aagatgtgat taaccaagca ttaaatcata tcgctcaata
     6061 cattaaattt tagtttgttg cttcacatag gatttgtgta gcttttatta tcaaccccgt
     6121 ttaacaggac gttagatgga tgtttatttt taagaagatt tattatgtca gaattatcgt
     6181 ttgctcaact acttgaagaa tcatttgcag ctacaccacg tttaggtgat gttgttaaag
     6241 gtactgttgt tgctatccaa aaaggcttag ttattgttga tacaggtttc aaatctgaat
     6301 ctgcaatcga tgcctcagaa tttactaacg cacaaggtga attagaagtt caagtgggtg
     6361 atgaagtaga tgttgttctt aaagctgtag aagacggttt tggtgcaaca gtagtttctc
     6421 gtggtgatgc aaaacgtaac gaagcttgga ttgctttaga gaaagcattt gaagaacaag
     6481 ctacagttat cggtttcgta aatggtaaag taaaaggcgg tttcactgtt gaattaaacg
     6541 gtgttcgtgc attcttacct ggttcattag ttgatactcg tccggttcgt gattctttaa
     6601 acctagaagg taaagagtac gaattcaaag taatcaaatt agatcaaaaa cgtaacaacg
     6661 tagtagtatc tcgtcgtgct gttatcgaaa cagaaagcaa ccaagatcgt gatgcggtat
     6721 tagcaaactt agttgaaggt tctgaagtta aaggtacagt taaaaactta actgactacg
     6781 gtgcattcgt agatttaggt ggcgttgacg gtttattaca tatcacagat atggcttgga
     6841 aacgtgttaa acacccaagt gaagtggtta atgtaggcga tgaagttact gttaaagtat
     6901 taaaatttga caaagacaaa actcgtgtat cattaggctt aaaacaatta ggtcaagatc
     6961 cttgggctgt aattgctgaa aaccacccgg taaacagcaa attaactggt aaagtaacta
     7021 acttaactga ctacggttgc ttcgttgaga ttttagacgg tgttgaaggt ttagttcacg
     7081 tttcagaaat ggattggaca aacaaaaaca ttcacccatc taaagtggtt aacgtaggcg
     7141 atgtagttga agtaatggta cttgaagttg atgaagaacg tcgtcgtatc tcattaggtt
     7201 taaaacaatg caaagcaaac ccttgggaac aatttgcggc aactcacagc aaaaatgata
     7261 aagtatcagg taaaatcaaa tcaattactg acttcggtat tttcatcggt ttagaaggtg
     7321 gtattgacgg tttagttcac ttatctgaca tttcttggaa tgtttcaggt gaagaagcag
     7381 ttcgtaacta caaaaaaggt gatgaagtag aagctgttgt tctacaagtt gatgcagtga
     7441 aagagcgtat ctctttaggt atcaaacaat tagaatctga tcctttcaca aactttgtag
     7501 atggtactaa aaaaggtgca atcttaacag gtaaagttgt agaagttgat gctaaaggcg
     7561 ttaaagttga attggaaggt ggcgttgaag catttattcg tgcaaacgaa gcaactgctg
     7621 agcgtgtaga agacatcact tctgttattt ctgttggcga ttcaatcgaa gcgaaataca
     7681 ccggtgttga tcgtaaatct cgcgtagtta acttatctgt tcgtgcaaaa aacgaagctg
     7741 aagaatcagc agcaattgca caagtgaaca aagaggaagt tgttgtacct aatgcatttg
     7801 ctgaagcttt caaagcagct aaaggcgaat aattagtata gactaatcta aaatgggtat
     7861 gttttgaaca tacccattta aacttagaaa tgccattttg aatacttatt ttcataataa
     7921 atgattattc aaaatagaat taatttttgg tagaaataat atgactaaat ctgaattaat
     7981 tgagagttta gcatctaaaa atccaagttt accaattaaa atggtggagc attgtgtaaa
     8041 agagctttta gagcagttaa cggctacttt agaagaaggt gaaaggatag aagtaagagg
     8101 atttggtagt ttttctctac attatcggca gccgcgttta ggtcgcaatc ccaaaacagg
     8161 agaaagtgtt cttttaggcg cgaaatatgt accacatttt aaagctggta aagatctaaa
     8221 agagagagtt gatctgcttt aagtcttatt aattcaataa tcgtagtaag gagattatat
     8281 gattaagtat attttaggta ttttaattgt gattgcagtt attattgtcg ctgttacgat
     8341 tggagccaat aatgatcaac ttatcacctt taactatatt tttgctcaga gtgaattacg
     8401 cttatcaacg ttagttgcga ttttatttgg cttaggattg attcttggtt ggttggtaac
     8461 gggtattttt tacttaaaaa ttaagtttca aaacgttgct ttaaatcgtc gtgtaaagcg
     8521 ccaagctcag caaataactg aattaacgac tccaaaggct gaataatcaa tgcttgaatt
     8581 attgttcctt ttattgccta ttgcagcact atatggctgg tatatgggac aacgtagtgc
     8641 aaagaaggat caagaaaccg ctagtaataa gttctctcgt gagtatgtta cggggttaaa
     8701 ttttctttta tctaatcagc aggaaaaggc ggtggacctt ttcctctcta tgttacaaaa
     8761 acaagaatcc gaaaatcata ttgcatctga atcccaattt gaagcggagt taaccctagg
     8821 taatcttttc cgttcaagag gtgaggtaga tcgtgcctta cgtattcacc aaacattagt
     8881 gaatagccct cattattcat ttgatcaaaa attattagca aaacagcaat tagctaaaga
     8941 ctttatggtt gcaggtttct atgaccgtgc agaaaattat tacatttcat tagttgatga
     9001 acctgagttt gctaaatatt ctttatctca gcttgctgtt atttaccaaa aaaccagaga
     9061 atggaaaaaa gcgattaatg ttacagaaaa acggcttcga atagagccgg atgcagataa
     9121 aataccgctt tcacattttt attgtgaata tgcgcaaggg gttaaatttg atgataaagc
     9181 ggcatttatt gaatcgttaa ataaagcgtt agaatattat cctcaatcca cacgcgcttc
     9241 tataatatta ggtgatttct atttagaaga gggagaatat ctaagagcat taagctattt
     9301 cgaacagatt cttgtgcaag accctgatta tattagcgaa gtgcttggac gaattaagca
     9361 aagctatatg gctcttgagg acttaaatgg ctatgagtta tttctcattc gagcaaaccg
     9421 agtaaaacat aatagtagtg tagatattgc tttaactgaa tttattgaag aaaaagatgg
     9481 tatcaatgct gcacattcta aactatacca gcagttgagt acttatccga atttaattac
     9541 ttttcaccgc tttattcgct atcaagcaga ttttgcggag gaaggaaatg gtaaggaaag
     9601 cttagtttta ttacataata tggtgggaaa tcagataaaa cgcagcttcc aatatcgctg
     9661 tttaaattgt ggttatcaaa gttatcgctt aatgtggcaa tgtccttctt gtaatcaatg
     9721 ggaaaaaatt aagccggtac aaagtatcga aggtattatt caataaagaa agaatctgag
     9781 gaattgaaaa tgaacaataa agtaattgtt gcgttagact atgaaactga acgtgaagca
     9841 ttagaatttg tggatttggt cgatccgtcg ctttgtcgtt taaaagtagg caaagaaatg
     9901 tttacaacct taggcacaaa ttttgtcaaa caattacacg aacgtaaatt tgatgtattc
     9961 cttgatttaa aatatcatga tattccaaat acggttgctc gagcagtacg ttctgctgct
    10021 gatcttggtg tgtggatggt tgacttgcat gcttctggtg gtttagccat gatggaagag
    10081 gcaaagaaaa ttttggagcc ttatggcaaa gaggcaccat tattaattgc agtgacggtt
    10141 ctaaccagta tggaagattt ggatttgctt caaataggta ttaatgcttc gccgatggaa
    10201 caagttattc gtttagcaca tttagcacaa cgggctggtt tagatggcgt ggtatgttct
    10261 ccacaagaag ttgaggtatt acgtacccat tgtggtcaag attttaaatt agtgacccca
    10321 ggaatccggc caaaaggaac agattttggg gatcaacgcc gagtgatgac acccaaacaa
    10381 gcagtagaaa caggctcaga ttttttggtt attggtcgtc ctattaccca agctcaagat
    10441 ccgcttgcgg tattaaaatc tattaaccaa tctatcagtt aattcattgc atttttaatg
    10501 aaggcggaaa aatttccgcc ttttttaatc aaacaagcgg ttatttttga ggaaaaattt
    10561 gtaaatgaca ttagtctatt caacggaaac aggcagaata aagtccgaag taataaaaga
    10621 agaacgccct aaaggggatg gtattgtgcg tattcaacgt caaacaagcg gtagaaaggg
    10681 gaaaggtgtt tgccttatca ccggtcttga tttagaggat aaagccttaa attctcttgc
    10741 ttcagagctt aaacgtaaat tgggttgtgg tggctcattt aaagatggag taattgaaat
    10801 ccagggcgat aatcgaggat tcattaagca aatcttagaa cagaaaggtt tcaaagtgaa
    10861 actggcaggt ggttgagttt taaacaatta tcagctggca gtttttaaat aacttcattt
    10921 tatttgaaac ttcattccta cttttctgcc taaaactcac gattaaggag aaaaactaat
    10981 gtggaagaaa acgttgattg ctgtattttt agcaagccaa tatgcttttg cggtaggaac
    11041 cgggaaagaa ccatcaaaaa gttatactga aacagaatta aaacaattaa aaaatgattg
    11101 gttagcgatt tatcaaaaag agctacagta tgcagaaaat ttgcgtttaa aacagagaga
    11161 aaattttcag caaattaatt atttggcaga tgcagcgatt agtcagaaga atttatcact
    11221 ttcaacggag cagataattc agcgtttatt actaacctta gcgggttatc cgttggagat
    11281 gaaagctgaa tggaagctgc taaatgctaa aataacgtta aaacaagcca cagaaagtga
    11341 agttaaagca tttatttcaa aatatcctga ttctttgtat caaaggcagt tacagaactt
    11401 gatgtttgag ctgctctatc gtgaacagaa atttccggag ttactggaat atgctcaaaa
    11461 tgtaaaacag agtagtcttg ccgaccaatg ccgtttattt tctgctcgtt atgaactggc
    11521 agcaagtaaa gctcaaatca atccggaagt ttctcaagta actgagcaaa aagcagaaaa
    11581 tgcagaactg cttgctttgg tgcaagagtt tgatcgcttt tggcttgcta cgccgaaatt
    11641 aacttctgat tgtgcgaatt tagaaagtta ttggcgggat caggggctta aaaccgatga
    11701 aaaaatgcgt ttgaaagcgg tcgaattaat taaacaaaat gcaaatgcag aattggcgaa
    11761 tttggcggca aatgtaaata atgagaattt gaaaatctgg ctatcttccg ttgaaaaggt
    11821 agcgaataat ccggcagatt tgcaaaattt tatcgaaaat caaccgcttg attccccatt
    11881 ttatgctgat aataaagcga ttattcagca actctttgct aaatatgtgc gaactttatc
    11941 ggaaaatatg gagaacccga gtttccagca gtatcaaacg tgggcagaga agtatcaact
    12001 ttccagcgag gaaatcaatg gctggaaaaa tgcttttatc actcgatttt ttgataaccc
    12061 agaaccaaca ttccaatttt ggcgtgatga gcaaatcaag ttgctcaaga cagacaattt
    12121 aactgaacgc cgtttacgaa tggcaatttg gcaaaaagca gatttaaatg agtggctggt
    12181 tctgctatct cagcaggcga aagaaaaagc agaatggcgt tattgggctg cgaaagagga
    12241 gaaaaacgaa gcgaagcgta aagttgattt tgaaagctta gctaaagaga gaggctttta
    12301 tgctatgttg gctgctcaac aattaggcat tgtttaccaa atcccgatgc ttgaagtgcc
    12361 tgaattgaca caagcacagc ttgaattgta tgcaaaccaa ttagctcgta ttcaagagtt
    12421 gcgagagcta gggcagtttg accaggcaag aaaaatatgg attgactgga taaaaaacat
    12481 caagccggaa gcaggtgaaa atacaccaac taaaatccag ttagccctga taaaatatgc
    12541 caaggataaa aattggtatg atttggcggt ggaaggtaca attcaaacaa aagcctttga
    12601 ttatcttgat ttgcgattac cgaatgccta ttcggattgg ttcgatttac atctgcaaga
    12661 taaaaaaatc agcaaaacgt ttgctcaggc gattgctcgt caagaaagtg cgtggaatgc
    12721 tcaggctcaa tcgcacgcta atgcgagagg gctaatgcaa ctgttaccga caacagcaca
    12781 aaaaaccgct aaagataacg gtttaacctt caaagatggc aatgatctct ttcaaccgtt
    12841 taacaatatt atgttaggca caacccattt aatggagtta aacgaaaaat atccgaataa
    12901 tcggattctg attgcctctg cttataatgc aggagcaggg cgtgttgagc agtggctgaa
    12961 acgtagtaac gggcagttag caatggatga atttattgcc agtatcccgt tttatgaaac
    13021 cagaggctat gtacaaaatg tgctggcgta tgactactat tatcaaatgc tctatagcga
    13081 tataaatgat aaaaatggat taaaaatgtt ctatcaagag gaattaacaa gaaaatatta
    13141 aaaaatgctt gtgtttttgt gtagaaccgc tataatgcac cccatcagac gcggggtgga
    13201 gcagcttggt agctcgtcgg gctcataacc cgaaggtcgt tggttcaaat ccggcccccg
    13261 caaccagttt taagctcaca gctttggtta aagttgtgag ttttgtttta tatgctgtag
    13321 caaatttgca aaaaatttgg caaaaaagac cgcttgttgt ggttgctaca gcaaaaaaca
    13381 aaccgcttaa agccccacgg tttcccgtct ggggcttttt tgcggaaatt ggtacagcat
    13441 taacgtatca aagctggttt atccggcttt ttttatattt ggagattatg acttggcaac
    13501 tttagaacaa aaattagaag atttagtgct cgattctatt gaagcattag gctttgaatt
    13561 agtcggcatt gaatgccaac gtgcaggccg ttttttaacg gtgcgtttat atattgataa
    13621 agaaggtggc gtgacgattg atgattgtag cgatgtcagc cgccaagtga gtgcgatttt
    13681 tgatgtggaa gatccgattg ccgataaata taacttagag gtttcttcac cagggttaga
    13741 tcgtccatta tttaccgttg cccactatga gcgttttatc gggcaagatg tagtgattca
    13801 tttacgcatt ccgatgttcg atcgccgtaa atggcaaggt aaaatcaaac aagtagaagg
    13861 tgatttcatc actttaacgg tagaaaatga agatcgccag ttcgcatttg gcaatattca
    13921 aaaagcaaat ttagttccgg tttttaattt ttaagataaa ggtaaactga caaaatgagt
    13981 aaagagattt tattagctgc ggaagcagtt tcaaacgaaa agcaattacc aaaagaggca
    14041 atttttgaag ccttagaaac cgctttaacc attgcaacca aaaagagccg tgatttagat
    14101 attgacgtgc gtgtggtggt agatcgtaaa accggtgatt tccaaacttt ccgtcgttgg
    14161 gctgtggttg aaaatgtgaa taatatgacc cgtgaaatca gcttagaagc ggcacaattt
    14221 gaagatccaa acgtacaatt aggcgatttt attgaagatg aagtagagtc tatcgagttc
    14281 ggtcgtgtgg cgattcagac ttttggtcag gtagtcaaaa ccaaaattcg tgaagcggag
    14341 aagacgaaaa tcattgatga attccgttcg gatttaggca aaattattct tgccactgtg
    14401 aaaaaagtaa cccgtgataa tattttcctt gagttacaag gtaaatcaga agatatcaaa
    14461 ggcgaagcgg tcattgtgcg tgaagatatg atcccacgtg aaaacttccg cccgggtgac
    14521 cgtgtgcgtg gtgtgttata cgaaatcaag cctgaatcaa aaggacctca gcttttcgtt
    14581 acccgtgcta aaccggtgat gttagaagag ctattcaaat tggaagtgcc tgaaattggc
    14641 gaaggcgtaa ttgagatcaa aggttcagcc cgtgatgtcg gctctcgtgc aaaaattgcc
    14701 gtgaaaaccc acgataaacg tattgacccg gtaggggctt gtgtgggtat gcgtggtgct
    14761 cgtgttcaag ctatcagcaa tgaattaggc ggcgagcgtg tggatatcgt actttgggac
    14821 gaaaatccgg ctcaatttgt gattaatgca atggctccgg cagatgtaag ctctattgtg
    14881 attaacgatg aaactcactc aatggatatt gcggttgaag cgaaaaatct ggctcaagct
    14941 atcggtcgta acggtcaaaa cgtgcgtttg gcgacacaat taactggttg ggcattaaat
    15001 gtaatgaccg ttgaagagct aacgaagaag agcgaagaag aagatggtaa agtcatcaat
    15061 ttattaactt ctgctttaga aattgatgaa gaatttgctc aagttttaat tgaagagggc
    15121 tttacgtctc ttgaagcgat tgcttatgtg ccggttgata ctttaacggc gattgacggt
    15181 ttagaagatg aagatttagt cgaagaatta caatctcgtg ccaaaaatgc gatcacgaaa
    15241 aaagcgttag aagaagagaa gttattaaaa tcagctcaaa ttgaagaaaa attattaacg
    15301 cttgaaggta tggatcgcca tatcgcattc aaattagctg aaaaacagat tacgactctt
    15361 gaagaacttg cagagcaagg tgttgatgat ttagccgata ttgaagaact ttcagaagag
    15421 aaagctggcg agctaattat ggcagcacgc caaatttgtt ggttcaacta agcaaagagg
    15481 ataataaaat gacagaagag aaaaaaacac tttcgcttgg tggcgctcgc aaagcgagta
    15541 aagtgaatac aaccacaacc agtggtaaag taaaagcggt tgaagtaaaa gagaaaaaac
    15601 cgaaaattga tgcaaaagca ttgcaagaaa aagcagcaaa agaagctgcc gcagcgaaag
    15661 ctagagctga aaaagaagca gctgaaaagt tagccaaaga aaaagccgaa gctgaagcta
    15721 aagagaaagc tgcaaaaaaa gcagcagaat cgaccgctag taaaccgatt tctgtgccgg
    15781 taatgccaag cagcaaagca aaagaagaaa aaccgaaagc acaacaggta cagccaaaac
    15841 aagagaaagc ggttgatcct gaaaaagaag ctaaacgcaa agaagaagct gagttacgcc
    15901 gtaagcaaga ggaattagct cagcaaaaag cggaaatgga ggcaaaacgt gctgcagaaa
    15961 atgcccgccg tttagcagaa attgctcgtg aagaagcgga agataacggc aacgaagatt
    16021 ttgatgatga gcgttttact tcttcttatg ctagagaggc tgagcgtgat aatgaccgcc
    16081 gtagtgaggg aaatcgtggt cgtaacggca aaggcaatgt ggctaaagcg aaaaaaggcg
    16141 gacgtgaaga ggataaaaac gaacgcagtg cagatcgtcg caaccataaa gaagtaaaag
    16201 gcaaaggtaa acaaggtaag aaaggtgcag ctttacaaca agcctttacc aaaccagctc
    16261 aagttgttaa agctgatgtg gtgattggtg aaactatcac tgtggctgaa ttggctaata
    16321 aaatggcagt gaaagcgact gaaatcatca aaatgatgat gaaaatgggt gaaatggtga
    16381 cgatcaacca agttatcgac caagaaaccg cacaattagt ggcggaagaa cttgggcata
    16441 aagtgatttt gcgtaaagaa aatgagttag aagaagcggt tttagaagat cgtgatactc
    16501 acgcagaaaa agtaacccgt gcaccggtag tgactattat gggccacgtt gaccacggta
    16561 aaacctcgtt acttgactac attcgtaaag ccaaagtggc ggcaggtgaa gcaggtggta
    16621 ttacccagca cattggtgca taccacgttg aaaccgatga cggcaagatg atcactttct
    16681 tagatacccc ggggcatgca gcctttacct caatgcgtgc gcgtggtgcg aaagcaactg
    16741 acatcgttgt tctcgtcgtt gcggctgatg acggtgtaat gccacaaact attgaagcta
    16801 tccagcacgc caaagcagcg ggtgcgccat tagtggtagc ggtgaataaa attgataaac
    16861 cggaagcgaa ccctgaccgt gtagaacaag agttactaca acacgaggtt atttctgaga
    16921 aattcggtgg cgatgtgcaa ttcgtgccgg tatctgcgaa aaaaggtatg gggatcgatg
    16981 atttactaga agccattatt ctgcaatccg aagtgcttga attaaccgca gtaaaagacg
    17041 gtatggcaag cggcgtggta atcgaatctt acttagataa aggtcgtggc cctgtggcaa
    17101 ctatcttggt tcaatccggt acgctaaaca aaggcgacat tgttctatgt ggttttgaat
    17161 acggacgtgt gcgtgcaatg cgtgatgaaa acggtaaaga tgttgaatca gcaggtccgt
    17221 ctattccggt agaagtatta ggcttatcag gcgtgcctgc tgccggtgat gaggcaaccg
    17281 tcgtgcgtga tgagaagaaa gcccgagaag tggctcttta tcgccaaggt aaattccgtg
    17341 aagtaaaact ggctcgtcag caaaaagcta aactggaaaa tatgttcagt aatatggcag
    17401 ccggtgatgt ggcagaattg aacatcattg ttaaagcaga cgtacaaggc tcggtagaag
    17461 cgatcagcca atccttaatg gaactttcaa ccgatgaggt gaaagtgaaa attgttggtt
    17521 caggtgtagg tggtattacc gaaactgatg caaccttagc cgcagcttct aatgctatta
    17581 tggtcggctt taacgtgcgt gccgatgcct ctgcccgccg tgtgattgaa aacgaaaata
    17641 tcgatttacg ttactactcc atcatttatg agctattaaa tgacgtgaaa gcagcaatga
    17701 gcggtatgtt acagcctgaa ttcaaacaag aaattatcgg cttggcagaa gtgcgcgatg
    17761 tgttccgcca cccgaaattt ggggcgattg ccggttgtat ggtgacagaa ggtatcgtta
    17821 aacgtaacaa cccaatccgt gttctgcgtg ataacgtagt aatttttgaa ggcgagttag
    17881 aatctctccg ccgtttcaaa gatgacgtat ctgaggtgcg taacggtatg gaatgtggta
    17941 tcggcgttaa aaactacaac gacgtaaaag ttggcgacca aatcgaagtg tttgaagtgg
    18001 ttgaagtaaa acgtactatt tagcctaatt ttgtgtaggg gcgtattgca tacgcccttt
    18061 gtatttttta gggcgtgaag catcacgccc ctataaaaga gaaaaatatg tccagagaat
    18121 ttaaacgtag tgaccgtgtg gcacaggagc tacaaaaaga agtggcggtg attttacaac
    18181 gtgaggtaaa agatcctcgc attggtatgg tgactgtttc tgatgtggaa gtatcaagag
    18241 atttggcgta tgccaaaatt tttgtgactt tcttatttga taacgatgct gaagctatta
    18301 agcaaggtat gcaaggcttg gagaaagcaa gcccctatat tcgaaccctt ttaggtaaag
    18361 caatgcgttt gcgtattgtg cctgaactgc gttttatcta cgatgaatcg ctagtcgaag
    18421 gtatgcgtat gtctaacctt gtgtctagtg taatcaaaaa tgacgaagaa aaacataaag
    18481 aggactaatt gtgtctagac ctcgcaaacg tggccgtgat attcacggcg ttttcttatt
    18541 agataaacca caaggaatgt cttcaaacga tattctgcaa aaggtaaagc gaattttcca
    18601 agcaaataaa gccggacata ccggggcgtt agaccctctc gcaaccggaa tgctgccgat
    18661 ctgtttaggt gaagcgacca aattttcgca atttttgttg gattcggata aacgttatct
    18721 cgtcaccgca aaattaggcg agcgaaccga tacttcagat gcggaagggc aagtggtgga
    18781 aaccaaagca gttgcagtga caaaatctca aattttggct gctttggaga aatttagagg
    18841 tgatattttg caagtgccga caatgttttc ggccttaaaa cacaacggca aacccttgta
    18901 tgaatatgcc cgagcaggga ttacggtgga gcgtgaggct cgcccgatta cgatttttga
    18961 actaaatttc attgaataca aggctccatt tttaaccctt gaggtgcatt gttctaaagg
    19021 tacttatatc cgaactttgg tcgatgattt aggcgaagag ttgggctgtg gtgcacacgt
    19081 tacgatgcta cgccgtacag cagtggcaga ttatccgatt gaaaaaatga tgagctatgc
    19141 agatttgcaa aatttagccg aaaatcaacc gcttgcagag ttagaccagc acttattgcc
    19201 gatggatacg gcagtttctg cattagcaaa aataaattta agttcagagc aaactaaagc
    19261 ggtgggtttt ggtcagagaa ttagatttga taatctgaac caactttatg gtttggtacg
    19321 tttatattca gaagatgaac aatttctcgg tgtggcagaa atcaccaatg atactgtgat
    19381 tcgtcctaac cgaatgatta acttaacgga ataagaatga aatcgataat ttttacctta
    19441 cctttagcga ttatcacttt agccttagcc ggttgttcaa gtgataatca agaaagttat
    19501 gccggcttaa acttagatgc taaatacagt aaatctcgct cggtcagtca ttttaatgat
    19561 tatgtgcatt ttctgaaaca gaaggcagca ggggcaggag tgtctgcaaa aaccttaaat
    19621 gctcagaaat tcattcatta caacgccaga gcggtgcagt tagatcaaca gcaagctgca
    19681 cgaagacgtg acccgaatgt accgcctccg ccaccaaatc caagttttaa acaaagtatt
    19741 aactcaagcg aaggtggatc aggcaagtga acgttggtat gaatataata gccagcttac
    19801 ccatgccagc caaaaatatg gggtacaaaa agagtatatt ttggcacttt ggggaatgga
    19861 gagcagcttt gggcgtaacc aaggggattt tgatgtactt tcggtgttgg caaccctcgc
    19921 ctttgatggt cgccgtgaga agttatttac gcaagaattt gtgaatgcga tgaaaatgct
    19981 cgatgacggc acaattaaac gctatgaatt gcttggatct tgggcaggag cgatgggaca
    20041 aactcaattt atgccaactt cttacttaac ctatgcagct gatggcaata atgacggtaa
    20101 aaaagatatt tgggagaatg aagccgatgc atttgcctcg attgccaatt atctttcaac
    20161 agtaggttgg gataatcaac taccttgggg tgttgaggtt aaattagctt caccgatgga
    20221 tttaagtttc gccggtcttg aaaccaacaa agcgaaaaca ttggcggaat ggcaagcctt
    20281 aggtatttat ttagcttatc cgaatggaca agaatcggca aaattagctc aattacacgg
    20341 caaagaggtt tggttgattc gtccggataa agaagcggga agagcgtttt tagtttctaa
    20401 taatttccgt actttaatgg attggaataa atcgaataac cttggtatca gtatcggaaa
    20461 atttgccgat cgaattttag cttcagttgg tcaataatcg aataaaagca tagtagcaaa
    20521 gcctatctat gcttttattt tatcaaagcg tcgcctttct tctgcaaacg acgttgctta
    20581 aaaaaatcac tgatcttttg gctacattct ctgcccaata cattgccatg cacttctaag
    20641 aaatggttca ttttataatc ttcaaacaga tgatagcgtg aacctaccgc acctgtttta
    20701 taatcacttg ccccaaaaac caatcgctta atccgactat gtaaaattgc accggcacac
    20761 attgggcaag gctcgagcgt aacataaagg gtacaatcta acagacggta attccctaaa
    20821 gtttgtcctg cttgacgaat tgcctgcatt tcagcgtgtg cagatggatc acagagttga
    20881 atggtttggt tccagcctct gccgataatc tgttggtttt tatcaaccag tactgcccca
    20941 accgggattt cgccttttgc ttcggctaaa tcagcgagat caagggcgta ttgcataaag
    21001 gcgatgtctt gctcggagat ctgaagtgga caagaagttt gatttggttt gataaacata
    21061 gttgcaaaaa attgagtttt tttgaccgct tgtaaaatgc cccacgaggg tggggcatcg
    21121 cttacttcgc ttagtgtcgt ttagtcacga cgaccacgtt tttcactgaa acgtttttct
    21181 ttaaagccac ctttgcggtc tttattgaaa cccccgtctc ggcgaccacc acgatcatta
    21241 cgatcgttac cacggcgacc acgaccttca aatggatttg cattacttgc actgtttgca
    21301 gagccaagta atgtcatctg cattggtttg cttaatacac gtgctttgtg agcgaaatgt
    21361 tgtacgagat gatttggcat tccttgcggc aactcaatgg ttgagtattt atcataaagt
    21421 ttgatatgcc cgatgtaacg gctgctgata tccccctcgt tggcaatcgc tccaacaata
    21481 tgacgaactt ccacaccgtc ttcacgcccc aattcaatgc ggtataaatc cattgccaca
    21541 ccattgtttt cacggtgttc acgaccgtta cgacgatcgt ttcgctcatt gcggtcacgt
    21601 ccacgttcag aacgggcagc acgcacaacc ggatccggtg gcagaatcag tttttgtttt
    21661 tcttgcagta acatcatcat cgccgctgct aattcttcgt ggtcttgatc cgcagtaaat
    21721 aaatcttcca acaattcacg atatttttct aaatcgtgat gttctaactg cttagaaatg
    21781 cgggctttga atttctcacg gcgtttttcc attaaaattt cgtggtttgg aatcgccact
    21841 tcattaattg gttttttgat taaattttcg atattgcgta ataagcggcg ttcacgaggc
    21901 tcaacaaaca gtaaggcacg acctgaacga ccggcacgac cggtacgtcc gatgcggtga
    21961 acataagatt cttcttctaa cggaatatcg tagttaataa ccaagctgat tcgctcaata
    22021 tcaataccac gggcagcaac atcggtggcg actaaaatat ctaaacgacc ggagcgtaat
    22081 ttatctaagg tttgctcacg ggcttgctgg gtcatatcgc cgtttaatgc cgcagtacgg
    22141 tagccgttgc gttcacacaa ttcggtaata tcaatagtac cggttttggt gcgggtaaag
    22201 atgatagcag catcaaattc ttctacttct aggaaacgca ataacgcatc gtttttacgg
    22261 aagccgttta ccagccagta gctttgttca atatccgggg ctgaacgctc ggttgcctta
    22321 atttttacct cttgcggatc tttcataaaa cgacgggtaa tacggcgaat cggctccggc
    22381 atggtggcag agaagagagc agtttgatgt gtttccggta attcagccat taccgtttca
    22441 acatcgtcaa taaagcccat acgcagcatt tcatcggctt catctaacac gatgaatttt
    22501 aattcggata aatctaatgt gccacggcgg atatggtcaa gaatacgccc aggtgtaccc
    22561 actactactt gtgagccttg ttttaaagca cgtaattgaa tgtcgtaacg ttgtccacca
    22621 taaaccgtca ctacacgaat gcctttcata ttttttgcaa actgctcaca agcatcggct
    22681 acttgaatcg caagctcacg ggtgggtgcc atcaccaaca tttgcgggtg gcgttgtgtt
    22741 gggtcaattt gtgccaacag cggcagcgag aatgcggcgg ttttaccgct cccggtttgt
    22801 gccataccaa gcacatcacg accggctaat aaatgaggaa tacattcttg ttgaatgggt
    22861 gatgggttta caaagcccat ttcatttaca gcatcaagga ttggttgagg taagcctaaa
    22921 tcggcgaaag ttaaagattc agttgtcata aaaaaccatt tgtttggaac gcacaaagca
    22981 gaaaatatcg actatttttc tgcacagcca ttaatgaaaa ttatattgtt gaaatttagg
    23041 aagaaatgaa ataacaaagc aagtgatgct ttaaaaatcc cctgagtata agcggttaaa
    23101 tttacaaaat tttttgcgaa attaaccgct tgtattgtcc taatctttcc ctattcttta
    23161 tcaacaacgg gagtcgtttg agtcatacca cgaatttgtg ataactcaaa cagggcaaaa
    23221 cgatactcaa cgaagttaaa cacttgattt gcaagagcaa gacgaaagag gctgattgcc
    23281 tcatctttat gatctaattt gagttgttgt tttgctaagt agaaatacgt ttctgtcagt
    23341 atttctgcat attgtgagct attttcctta gcgtcacgtt ccattatagc acgaacttga
    23401 gctaaattga gcttccctaa aaaataattt acgatatttg ttccccagta atccgaagaa
    23461 agattattag ctcgcttttg taaattattt ttagcttctg ttggtgtaaa ttccagctca
    23521 ttaaaataga gccataatgc tcgataagga tctttcttat cttcttcata aaaatggaga
    23581 agatcacgct gggcttcaga atatctgccg ctataataga aagccaaacc tcgattgaga
    23641 taggtgtagc tatattcccg atctaattcc aatacggcat taaacgcatc aatcgaactg
    23701 tcgtaatcgc cttccagcag cagatacagc cccatatagt tataagctgc cgccatacgt
    23761 tgattgagcg aaatggcttg agcaaaatca taacgggcta acgaccataa acctaaactg
    23821 tcgtaaataa cgcctctttc aaaatagagg tctgcttgct cagagggcgt taatggggct
    23881 tcttccaata cttggctgag tcttaccacc attgcctctt gttcaaagcg aagttgagga
    23941 ttaagttctg ccaatgctaa gtttttaatt gaaaccaaat cggcataccg actggtacag
    24001 ccgcttagca ttaaaacaga aattaaattc agtaataaga aacggattcg aaacaactta
    24061 aaaagttgta ccattacagc atatccctca attaaggaaa attaaaaact gaataacgga
    24121 cactcaaagt ccgttattcg cctttattat tgaactagaa attattttct tgttctgttt
    24181 ctaccacatt ttcagctaca gtttcttctg catttgcctc gttggtatta ttgatgtctt
    24241 tcatcgtcag acgaatgcgt ccttgacggt caatttctac cactttcact tgtacttctt
    24301 gaccaacggt taagtagtcc gctacacgct caacacgttc gttggtgatt tgtgaaatgt
    24361 ggactaaacc ttctttgcca cctaagatgg aaacgaatgc accgaagtcc accacacggg
    24421 ttactttacc gttgtagatt tggtttactt ccacttcggc aacgatctct tcaatacgag
    24481 ccattactgc tttcgctgca ttgccgtcag ttgccgcaat tttcaccgta ccgtcatcat
    24541 caatgtcgat agaagtattg gtctcttcgg ttaaagcacg aattgttgca ccgcctttac
    24601 caatcacatc tttgattttc ttcggatcga tcttcattgt gtgaatgcgt ggggcatagt
    24661 cagaaatatc tgcacgaggt gccggaatgg cttgttccat tacacctaaa atgtgcattc
    24721 ttgcaccttt agcttggttt aaggcaatac gcataatttc aggggtgatc ccttcgattt
    24781 tgatgtccat ttgcagcgca gtcacaccgg tacgggttcc cgctacttta aagtccatat
    24841 cgcctaagtg gtcttcatca cctaagatgt cagaaagtac cacgaatttc tcgtcttctt
    24901 tcacgagccc cattgcgata ccggcaaccg ctgctttgat tggcacacct gcgtccatca
    24961 acgcaagaga cgcaccacac acagatgcca ttgaagaaga accgttagat tcagtgattt
    25021 cagacaccac acgcactaca tacgggaact cttcagcggt tggcatcacg gctaatacgc
    25081 cacgttttgc taaacgaccg tgaccgattt cacggcgttt tggcgagccg atacgaccgg
    25141 tttcgcccac agagtatgga gggaaattgt agtggaataa gaaacggtca gatttttcgc
    25201 cggtcaattc gtcaatgatt tgggcatcac gctctgtgcc caatgttgct actgctaatg
    25261 cttgggtttc gccacgggtg aaaagagcag aaccgtgggt gcgtggtaac acactggtgc
    25321 aaatatccaa tgcacgcacg gtatccaccg tacggccgtc aatgcgtggt tcgcctgcaa
    25381 taatacggct acgcacgatt tggctctcta atgcggtgat gatgtcgatg atagtccctt
    25441 cagaaacagt ttcatcttct gcggtaagtt gtgcaatcac ctctgcttta atcgcatcga
    25501 tttgctcgta acgaacttgt ttttctacga tacgataagc atcgccaagg cgtgtttctg
    25561 ctaatgcttt tactttgttg attaaatctg tatttggctc tggtgcaacc caatcccaac
    25621 gtggtttgcc cgcttcttta acaaattctt tgatgttttc gattacaacc tgttgttgct
    25681 cgtggccgaa caccaccgcc gctaacattt gctcttcggt taagatatcc gcttcagatt
    25741 ccaccatcaa cacggctttg tctgtacctg aaaccactaa atctaagcgg ctgatttttt
    25801 gctcgctggt ggttgggttt aatacgaatt gatcgttgat aaaaccgaca cgagccgcac
    25861 cgattggacc gttaaacggc acgccggata atgaaagggc agccgatgca ccgatcatcg
    25921 caaccagatc aggactgatt tgtgggttta ccgatactac ggtcgcaatc acttgaattt
    25981 cgttaaagaa accttctggg aaaagtggac gcacaggacg gtcgatcaag cgagcgatta
    26041 aggtttcacc ttcgctagga cgtccttcac gtttgaagaa accgcccgga atacgaccgg
    26101 ctgcgtaagt acgctcttga taatctacgg ttaatgggaa gaaatcttgc ccttctttta
    26161 cgtctttttt cgctactacg gtaacaaata cggttgtgtc gtccatactt gccattactg
    26221 ctgccgttgc ttggcgtgcg atagcaccgg tttctaaggt cacggtgtgc tgaccgtatt
    26281 taaactgttt tacaattgga gtcattgaaa ttttcctttt aaagaattta ctattattgt
    26341 cgttttattt ctctaaattg cgactagcaa aaaggctttt aagtaaaaca tttttgatag
    26401 ccgcctctca ataacaagaa ataaaacttg tctagtatag cgtgattttt ataaaaaatc
    26461 gcctattatt ttgatcttgc acctcagtta taaagaaatt caaaacttca gtgtaaaatg
    26521 gggattagtt tgtgaaaagc cttgtgaaaa tagtttgatt gcatcgctat ttttttatta
    26581 gaggtagttt aaaactaaat ctgcttcctt taccggcttc actttcaatt tccagtgtgg
    26641 aatggtgctg ttcaagagcg tgtttgacaa tagccagacc taaaccgcta ccgccggttt
    26701 tgttgctacg agattcgtcc acacgataga aacgttctgt cagatgggta atatgatggg
    26761 catcgatacc aataccgtca tcttgcacgc tgaaaatcgc agtattcggc tcttgattat
    26821 cacgttgaca acatatttga atggtaactc cttcaccggc gtgccgtatt gcattgtaaa
    26881 ttaaatttga tacagcgctt tggagttgct cttgatctcc ccataccgtt aaattaggct
    26941 caatatcaaa cagtatttgc tgattttggc ttaaaatctc ggtatttttt tgtatggcgg
    27001 agattaacgc cgacatctgg acctcattgt gttcggaatc attggaatgc tcgattttaa
    27061 ccaaaagtga gagttggcga agcaggttag tcatacgttg actttgctct ttcatcgcat
    27121 tgagcgcttt ttgctgtaat gccatattat gcggctcgtt ttccagcatt tccaaataac
    27181 cttgtaatat ggtaagaggt gttcgcagtt cgtggttcat atttgccaaa aaggtttgtc
    27241 gtgagtgcat aaggcggaca aaagaggtta catctcgtgc gacaataagt ctggagttac
    27301 tgtcatatcg attgagattc aattcaatat agcgtttatt atgagtcagc agtaccaatg
    27361 ggcgatggtg tcgttgttgg cggaaatagc ttttaaattc aggataaaaa atcacattaa
    27421 aaatgctttt atttgatttt ttgtcccaat aaaactcaaa tagctcttgt gcaacattat
    27481 tgcaccaaga aatctccccg ttttcttggc aaataatgat cgcatcgggt aagtattgaa
    27541 tatgcttatt taattttgat aacagacgta aggttttgat tttatcttgg cgtaaacgtt
    27601 cgtgatagaa tgcggcactt tgagataaat gctccattgt cgttactttt gacctgccgc
    27661 tttgctcatt aggtctgagt aactgaagga gagaagattc ggtgtggtga tgccaaataa
    27721 gtagaaccag taaacaaaaa ctaaaccaga aaaaataagc agggaaaaac agattgaata
    27781 cgccggcaac ggctgctgcc agcaaaagtt ccaataaaaa gtgtttaagt gagagcttaa
    27841 ctttcattat tagtcctgaa aatggttgga aaaacggtat cccgagccac gtacggtttg
    27901 aatataacgc tcaaaaccat agggttcaag actcttacgc aatcgccgaa tataagagtc
    27961 taccgtgcga tcctcaacat aaatatcatt accccaaata aaatcaagca actgttcacg
    28021 acgatacact ttttcagggt gagtcattaa aaagtgaagc agtttatatt ccgtgccgct
    28081 taatttcact tcctgttcgg caaccaaaac ccgttgtgcc tgttgatcaa gaatcaatcc
    28141 gtctatattg atgagattgc ttggcggatc atagcttcgc cgccatactg cttcaatccg
    28201 ggcaagcaac acctgtggtg aaaacggttt ggtaatgtag tcgtctgcac cggcattcag
    28261 accggtaata cagtcttctt cgctactgcg ggctgttagc ataatgattg gaattttttc
    28321 ggtttcaggc tgttttttta ggtatttaat taaatggata cccgaacgcc ccggcatcat
    28381 ccaatccagt aaaataagct cgggtttttc gcccagttta ttgatagcac tttggtagtc
    28441 ttccgcttca attacctgat attgtttgct gagatagaga cttagcattt cacgaatagc
    28501 acgttcgtct tcaacgatca gaattttgtt attcattctt cctctccgat gcggtgtgac
    28561 aagcggtcga tttatcgcag aaatttgcaa attttttact aaaaccgacc gcttgcagat
    28621 taccccattt tacctttaat gtaatcttcc gtctgccgga attttggttt gtcgaaaatt
    28681 tgtttggtat caccgaattc aatcagctcg cctagataca tataagcggt atagtcagaa
    28741 cagcgggctg cttgctgcat attgtgggta actatcgcta ccgtgtagtc gtgcttgagt
    28801 tcgttgataa gttcttcgat tttcatagtc gaaatcgggt caagtgcgga gcagggctca
    28861 tccagcagta acacctcggg tttcactgca atacctcgtg caatacagag ccgttgctgt
    28921 tgcccgccgg agagactgtc accattttgg tttaatttat gctttacctc attccataaa
    28981 gccgctttgg ttaatgccca ctctacacga tcgtttagct cggatttcgg caatttttca
    29041 aacagacgaa taccaaaagc aacattatcg taaatggaca tcgggaatgg ggtcggtttt
    29101 tggaaaacca taccgacttt ggctcgaata atcgaaatat ccgtatcgga ggtgagtaga
    29161 ttctctccat caagattgat ttcccccgtt gcacgttgat tcgggtagag ctcaaacatt
    29221 cggtttaggg ttcgcagtaa tgttgatttt ccacaaccgg aaggaccgat aaatgcggtc
    29281 actttatttt tggcaatacg catattgata tttttcagag cgtggaaatc gccataataa
    29341 aaattgaggt tgttaatatg taattttgtc tctaaatccg ttggtaacat atagagtcct
    29401 tattttgttt tgctgaaata cagacgagtg atgatattaa gagaaagcac aaacagagta
    29461 ataagcattg cacctgccca agccaatgta ttccagtcag cgaacgggct ggctgcgtat
    29521 tgataaatca ccacagggag gtttgccatt ggtgcattca tatctaaaga ggcaaactgg
    29581 ttggataatg cggtaaacaa tagcggtgcg gtttctccgg aaatacgggc aacagcgagc
    29641 aacacgccgg taataatgcc ggagcgggcg gcacgataag agatcattgt aattaccctc
    29701 catcgtgggc agccgagagc cgcagctgct tcacgcagat tattaggcac aagattgagc
    29761 atactgtcgg tagttcgaac aacaatcgga atcactaata ccgccaatgc taatgagcct
    29821 gcccagcctg aatagtgttt aacctgtgcg acataaatcg cataaataaa taagccgata
    29881 ataatagaag gagcagagag caacacatca ttcaaaaaac gggtgaattt tgccaatttg
    29941 gtataacgcc cgtattcgct caagtaagtg ccggctaaaa taccgattgg tgttgcaatc
    30001 agtgttgcgg tactgacaat cagtatagag ccgacaattg cattacgtaa tcctcctgtt
    30061 tcatttggtg caggagtcgg gcgggtaaac agttcccaag acagtgcagg gatacctttt
    30121 tggagtaaag taacaataat ccagaccagc caaaataagc caaataatac agcggaatag
    30181 gcaagactta acatcaagac attttttatt tttcggcaat aaaagcgtcg gttttggtaa
    30241 tttttcattc tcgttttcct tcctgtttac ccattcgtgc gattaataaa cgtgaagcgg
    30301 ctaaaacaat cgaagtaata aggaacagaa ttagccctag gctcatcaac gctgattttt
    30361 gtaatccgcc ggcttcatta aattcattgg cgatagcaga agcgatggag gttgaagggg
    30421 aaaataccga atcaggtagc ataaaggcat taccgatcac aaaggtaact gccattgttt
    30481 cgcctaatgc tcgacctaac ccgagcataa ttgcaccgat tactccggtt ttggtgtaag
    30541 gtagcacgac tttccaaata acttcccaag tggttgagcc taaaccgtaa gcagactctt
    30601 tcagtaaagg cggtacaaca ctgaaaacat cacgcataat tgaggcaata aagggaataa
    30661 tcataatcgc caataccaag ccggcactga atagcccgat cccgaaagga gctccgctaa
    30721 aaagcatacc gataaaggga agctgaccaa atgtatctat caaatacggt tgcacatatt
    30781 cttgaaataa gggtacaaat acaaacagcc cccacatacc gtaaataata gagggaatag
    30841 ccgctagcat ttctaccgct gtgccgacag ggcgttttag ccattgcggt gcaagttcgg
    30901 ttaagaaagt cgcaatgccg aacgagaccg gaacggcaat gactaatgca atgccggcag
    30961 aaatcaatgt gccgataatc ggcactaatg caccgtatcg gtcattcaca gggttccact
    31021 cgttttccca taggaatgat accccgaatc ggcttaatgc ttcccaactg ccaagcaata
    31081 atgaaaccag caccaccacc aacaaggcaa aaaccagcaa agcaaagaaa cgggttgtgt
    31141 gatgaaataa cccctctacc caaggatggt ttaagcattt gttatcaatt ttatgtttat
    31201 gcatagtgac aactcttttg caaaatgttg tgaaaaaacg accgcttgtt gttagttaag
    31261 ctccatacaa gcggtcgttt tgcattagta gagcgaattt ccttgtccat ctttaacttc
    31321 cgttttccat tgagtttgga ttttctctac tacattatcc ggtagaggca cataatccag
    31381 ttcggcagcc gcatctttac cgtgcttaaa tgcccagtcg aagaatttaa ataccccttt
    31441 agtttgctct ggtttgtcgc tatgttgatg aatcaagata aaactggcgg cggtaatcgg
    31501 ccaggctttt tctcccgctt cgttggttaa aataacgccc ataccggctg tttggctcca
    31561 atcggcgtta gcagcggcag cagcaaacgt ttcacgggaa ggctgaacaa atttaccatc
    31621 tgcattttgc atagaaaccc aagccagagc attttgttta gcataggcat attctacgta
    31681 accaatagaa tacttgagct gtttcacata agctgcgaca ccttcatttc cttttccgcc
    31741 ttgtccgacc ggccatttca ccgattttcc ttcgccgact ttctccttcc attcggcaga
    31801 gactttagaa agatagtttg tccaaccgaa agttgtgcct gaaccgtctg agcggcgtac
    31861 gactaaaatt gtttttgccg gcagggcgat atcttgatta agtgcgacaa ttgccggatc
    31921 gttccattgt gtaattttgc ctaggaatat atcggcaaga actttaccgg acagtttcag
    31981 ttcgcccgat ttaagttcag gaatattgac aaccggtaca gttcccccaa tgatggcggg
    32041 aaattgcaaa agtttgtgtt ctgctaatgt ttcgcctttc atcgggtcat cagaagcacc
    32101 gaaatcaatt gttttggcaa taatttgttg ctgaccgcca ccggaaccga tagattggta
    32161 attgatttta ttaccggtct ctttttcata taaacctgcc catttggcgt agataggata
    32221 agggaaagaa gcaccggctc ctgtgatggt ttgagcattt gctgcgacag aaagtgaaaa
    32281 agaaatgcct aaaacggtaa gtagtttaaa tcgtttggca atcatattgt tctccttaaa
    32341 gttagcgtga gttaatttca cgtgcttagt gtagaaaaag aatatgacaa aatgatgaca
    32401 atggtaaaaa aatcagaaaa aacgaccgct tgtgtttcag gctagactaa acaggctaaa
    32461 tcgtagaaaa tagccctcgt ttttttaata taaatatcga taaaaaatga ccacagaaat
    32521 caattatgaa ggcattgaac agatgccgat taagcgtttt accgaagatg cttacctcaa
    32581 ttattcgatg tatgtaatta tggaccgggc attgccgttt attggcgatg gcttgaaacc
    32641 ggtgcaacgc cgtattattt atgcgatgtc ggaacttggg ctaaacgcct cggcaaagta
    32701 taaaaaatca gcccgtaccg tgggggatgt attaggtaaa ttccatccgc acggtgatag
    32761 tgcctgctat aaagcaatgg tgctaatggc tcaaccgttc tcttaccgct atccgttagt
    32821 ggacggacaa ggcaactggg gggctccgga cgatccgaaa tccttcgctg caatgcgtta
    32881 taccgaatcg aaactgtcta aaattgcaga aattttattg ggcgagttag ggcaaggaac
    32941 ggtggattat cagccaaatt ttgatggctc gttggaagaa ccgaaatatc tgccggctcg
    33001 tttaccgcat attttgttga atggcacgat ggggattgcg gtgggaatgg caaccgatat
    33061 tccaccacac aatattaatg aattggctga tgccagtgtg atgttgctgg ataacccgaa
    33121 agcaacgctt gatgatgtac taagcgtggt acaagggccg gattacccga cagaggctga
    33181 aatcatcaca ccaaaagcgg aaattgccag aatgtatgag caggggcgtg gttctattaa
    33241 aatgcgtgcg gtgtggaaaa aagaagaggg tgaaattgtg atttcagcct tgccgcacca
    33301 agcctctcct tcaaaaatta ttgagcaagt agcaacccaa atgcggaata aaaaactacc
    33361 gatggtggac gatattcgtg atgaatctga ccacgagaac ccgattcgca ttgtgattgt
    33421 gccacgctct aatcgcattg attttgatgc gttaatggat cacctgtttg cgacgaccga
    33481 tttggagaaa agctaccgag tcaatatgaa tatgattggc ttagacggca agccggcagt
    33541 gaagaatttg ctgactattc tcaatgagtg gctgagtttt agacgtacca ctgtcactcg
    33601 ccgcctgaat taccgattag ataaaatttt aaaccgcttg catattttag acggtttgat
    33661 gattgcattt ctcaatattg atgaagtgat tgagattatt cgcaatgaag atgagccgaa
    33721 agccgagtta atggctcgtt ttaatctgac cgatgtgcaa gcagaggcga tcctgaactt
    33781 acgtttacgc cacttagcca aattggaaga gcatcaatta caggcggaaa aatccgaact
    33841 ggaaaaagaa cgtgatgaat tgcaactgat tttaggctcg gaacgccgtt tgaacagcct
    33901 gatcaagaaa gagattcaag ccgatgccaa agcatttgcc agcccacgcc gctcaccatt
    33961 ggttgaacga gcggagtcta aagcgattgc ggaaagtgac ttgactccaa ccgaagatgt
    34021 gactgtgatt ttgtctgaaa aaggctgggt acgttgtgcg aaaggacacg atattgatgt
    34081 tcaagcccta agccatcgag ccggagatgg ttatcttgcc cacgcaaggg gtagaagtaa
    34141 tcagcctgtg gtatttttag acagtacagg gcgagcttat gctctcgatc caacttcact
    34201 gccttcggca cgctcgcaag gcgaaccgct tacaggcaaa atcaccctac cggaaggtgc
    34261 agtagtgcag caagtgctaa tggcaagtcc agaaactaaa gtgctaatgg catccgattc
    34321 aggttacggc tttatttgca cctttgcaga tttagtttct cgtaataaag ccggcaaagc
    34381 ggttatttct ttaaccgaaa atgcaaaagt actgccgccg caattattgg aaaatgatga
    34441 aaatttatcg cttgtagcga tgagtaatgt tggcagaatg ttggttttcc cggtgagtga
    34501 attacctcaa ctttccaaag gtaaaggcaa taaaatcatc aatatttccg ccgcagcggc
    34561 aaaatccggt gatgaatatt tagcccgttt attagtcatc aaaccaacca actcgctggt
    34621 ctttgtgtcc ggtaaacgca aaatcacatt aaaaccgagc gatgttgata actaccgtgg
    34681 cgaacgagca agaaaaggct cacagctagt gagagggctt agtaccaatt caacggtaga
    34741 gattgttgag taaagagaag aagcggttag ggggtaaatc caacaatttt gccccaaata
    34801 accgcttgca atggagttat tgcttattca aataagcctt tgtgaagcga acgaaccaca
    34861 tcgtctgctt ctgcattttt cactaaggta caaacattat tgctgcttgc tccatagctg
    34921 attaggcgaa tgttgtaatt ctcaagagtg tggaataatt ttttcgccac accggactga
    34981 gcgtgtaagt tgttgccgat aatagcgact aaagcgaggt cgctttctac ttgcacattg
    35041 caatatgcgt taagctcatc gagtaattcc gctgataaca tatctgcacc tgaagaggct
    35101 gagcccgttt tatctagggt caatgcaacg ctgacttcgg aagtggtaat cacatcaacc
    35161 gaaattttgt gtttcgctaa aatcgcaaat acattcgcta agaaaccttg tgcgtgtagc
    35221 attgaaaggc tggaaagcgt gagtaaaatt tggttgcggc gtaatgcaat cgcacggaaa
    35281 gtcgggcgtg gttgtggatc acgggttacc caagtgccgc cttgttccgg tgctttgctt
    35341 gagccaacat atacagggat attgctacgc actgccggta ataaggttgc tgggtgtaat
    35401 actttcgctc caaatgttgc catttctgcc gcttcgttaa atgccattgt gtcaatgcgt
    35461 tgagcgttcg gtacaacacg aggatcggtg gtataaatcc ccggtacgtc tgtccaaatg
    35521 agtacatcgt tagcatttaa tacttctgct aacaatgcag cagagtagtc tgaaccgcca
    35581 cgtcctaaag tagtggtttt gccttcatca tcacgaccaa taaagccctg tgtaatcaca
    35641 attttgcctg atgcgattaa tggcttaatc acattgtctg attgttgctg ggttttctcg
    35701 tcatttggtg ctgcttttcc ataatggctg tttgttgcca caacgtcacg cacatctacc
    35761 catactgccg ggtgtccacg ttcaattaat aattgcgtga agattttggt ggacatcatt
    35821 tccccatggc tgattaactc atcacttaag gcagctgaag tagcaaggct ggctgattct
    35881 gctaatgttt caatacgttc taataattcg tcgatttccg ctttaacggc atcttgatgt
    35941 tgtaattttt caataatatt gtattgaatt tggcggactg ctgcgatgac ttcatcacgg
    36001 tgttcttttt cacagccgtt agcaagttcc actaagtaat tggttacgcc agcagaggca
    36061 gaaagtacca ccacacgagt atttgcatca gcaattacaa tattggcaga agaggtcatt
    36121 gcatcaaaat ttgcaacact tgtaccacca aatttagcca cagaaagatg agacataata
    36181 aaaatttcct taaaacaaaa atagataaat attgtgttag gggatattcg gaaaggaaat
    36241 ttgagtagat tatacggttt tattgttgaa ccaattaatc agagcactcc atttaatatg
    36301 gtgaaataac ctattaaatg acagctgata ggattcaacc tattcagccg atgttaattc
    36361 cagtctagtg aattcacacc tcggcaatca ctcccatcac ttaattctca aacggcccta
    36421 gttgcctaaa atcaagttgc ctgagtgttg cacctctccc gtaatggcaa gcccattact
    36481 ctttttcaac agtattgaaa agtgtgcata gaaataccgt tttattaatg ttttgtcaat
    36541 gaatatttag gtaatccaat gctatttagg taattattta aaata
//
LOCUS       DANLXS010000007        31658 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.007, whole genome shotgun sequence.
ACCESSION   DANLXS010000007 DANLXS010000000
VERSION     DANLXS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 31658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31658
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.007"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            74..1300
                     /locus_tag="PXD71_000269"
     CDS             74..1300
                     /locus_tag="PXD71_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDL6290436.1"
                     /translation="MAMPSQRFSLTWVLNLFGTAVGAGVLFLPINAGMGGFYPLIIMT
                     LLVGPMTYLAHRGLTRFVLSSKYKGSDITAVVREHFGEQAGKLITLLYFFAIFPILLI
                     YGVGITNTVSSFMENQLHIAPPSRAVLSFMLIAAMIGVMLLSEQVMLKITTCLVYPLV
                     LILLGLSIYLIPQWNTAALQQMPTTGDFLTTLWLTIPVLVFAFNHSPAISSFALSQQK
                     YYQDDKKAEIESAKVLRSTAFILVLFVMFFVFSCVLTLTPEELAQAKVQNISILSYLA
                     NKFDNPIISYFGPLVAFLAIGSSFFGHYLGAREGLEGLVNQMRKEPIDPSKFRKITAI
                     TFLIILWIVATINPSILGFIESLGGPIIAMILFIMPVYAVYKVPALARFKGEFGHLFV
                     LVMGCIAISAIVYGLL"
     gene            1357..2733
                     /locus_tag="PXD71_000270"
     CDS             1357..2733
                     /locus_tag="PXD71_000270"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDL6290437.1"
                     /translation="MISVFDMFKVGIGPSSSHTAGPMKAAKEFVDELQNRKFLNKIDR
                     LRVDVYGSLSLTGRGHNTDIAIIMGLMGYLPHNVDIERIEQTIENLKQNKQLIIAESS
                     VEPKVIAFDFYEDMPFHRDFLPRHENGVKFSALSGEELLFQNTYYSIGGGFIVDEANF
                     EQVKNSDLSVPFPYQNAADLLAHCKNNGLPLSSLVWKNELALRSRVEISDYLAMIWNT
                     MEACIERGLNTEGLLPGPLKVVRRASGLRRMLEANSKFNTDPMQIIDWVNMFALAVNE
                     ENAAGGRVVTAPTNGACGIVPAVLAYYQKFIAQLNQDTIERYLLTTSVIGSLYKMNAS
                     ISGAEVGCQGEVGVACSMAAAGLTEIMGGSAEQVCMAAEIAMEHNLGLTCDPVGGQVQ
                     VPCIERNAIASVKAINASRMALRRTSSPQVSLDKVIETMYETGKDMNAKYRETSTGGL
                     AIKILPCD"
     gene            complement(2788..4377)
                     /gene="purH"
                     /locus_tag="PXD71_000271"
     CDS             complement(2788..4377)
                     /gene="purH"
                     /locus_tag="PXD71_000271"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL6290438.1"
                     /translation="MAIQQALLSVSDKKGIVEFARGLSARGVKLLSTGGTAKLLAEAG
                     LLVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGTDDEVMNQHGIERIDMVVVNLY
                     PFAQTVANPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQHQ
                     NSLTLETRFNLAIKAFEHTAQYDSMIANYFGQKVPPYQGAEEEDLTQPSGQFPRTLNL
                     NFVRKQTMRYGENSHQNAAFYVENELKEASVSTAKQLQGKALSYNNIADTDAALECVK
                     EFSEPACVIVKHANPCGVALGKNILDAYNRAYQTDPTSAFGGIIAFNRELDGETAQTI
                     ADRQFVEVIIAPTVSASAKEVLATKKNVRVLECGEFSEAVKRLDFKRVNGGLLVQDAD
                     QGSVSIDDLKVVSERKPTEAELKDLLFCWKVAKYVKSNAIVYAKDGQTIGIGAGQMSR
                     VYSAKIAGIKAEDEGLQVVGCVMASDAFFPFRDGIDAAAKVGITCVIHPGGSMRDQEV
                     IDAANEHGMAVVLTGMRHFRH"
     gene            4549..4818
                     /locus_tag="PXD71_000272"
     CDS             4549..4818
                     /locus_tag="PXD71_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL6290439.1"
                     /translation="MINHNLADFVFEYFTENNLKAGTLRYRYIKENVIDAYSTRVDDA
                     FQGKGIAGELYNALIAFAQEKGLKIKPSCSYIEVKMQRSHRDLIA"
     gene            complement(4905..6350)
                     /gene="dacB"
                     /locus_tag="PXD71_000273"
     CDS             complement(4905..6350)
                     /gene="dacB"
                     /locus_tag="PXD71_000273"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDL6290440.1"
                     /translation="MIFKNFTRAFLNKFILSTLLIPSITQAEINTEELLNTLPAGTSV
                     SFIAKNLDTNQIITQYQSDIFMLPASTQKVFTALAAKLTLNDDFRFQTALLTNGKVEN
                     GVLKGNLIARFSGDPELTSGQIYQLMSKLKQQGINKIEGDLILDPSVFASHDKASGWI
                     WNDLTMCFNAPPAAINVDHNCFYVTLNADQPIGEFAKVNVPSAYPVQVFSSAYIVEPK
                     EAPFCQLDVVVHDNNRYQIKGCMARQSQPFGLSFSVQDPTNYGANMLKAQLKSLKIAF
                     NGQVKEPLTAQNGTLLAEHYSEPLPVLLKKMMKKSDNQIADALFRTVANKQHNRPASF
                     QLGSYVIRQLLKTKANIDFKNSVVADGSGLSRHNQVSSRTMLETLEYIAQNEESLKLF
                     ETFPIAGVDGTISGRGSISTEPLAKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFI
                     NGYSTGELESKTKRAPLNTFENKFYMALFNE"
     gene            6447..6923
                     /gene="greA"
                     /locus_tag="PXD71_000274"
     CDS             6447..6923
                     /gene="greA"
                     /locus_tag="PXD71_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL6290441.1"
                     /translation="MKQIPMTVRGAEQLREELDFLKNVRRPQIIDAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVTKMANNGKVIFGATVQLVNADTDEE
                     VTYRIVGDDEANIKEGLISVNSPIARGLIGKEVDDSVSITTPGGKVEFDIVGVEYI"
     gene            complement(6979..7395)
                     /gene="ndk"
                     /locus_tag="PXD71_000275"
     CDS             complement(6979..7395)
                     /gene="ndk"
                     /locus_tag="PXD71_000275"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL6290442.1"
                     /translation="MIQQTLSIIKPDATRRNLIGKILAQLEENGLTIKALKKVHLTRE
                     QAEGFYAEHKGKDFFEPLVEFMTSGSIVVSVLEGENAISHYRALMGATNPDNREKGTI
                     RDLYALSMRENSVHGSDSEESAKREIAYFFVPSEIV"
     gene            complement(7405..9483)
                     /gene="metG"
                     /locus_tag="PXD71_000276"
     CDS             complement(7405..9483)
                     /gene="metG"
                     /locus_tag="PXD71_000276"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL6290443.1"
                     /translation="MANQKRKMLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGN
                     EVHFVCADDAHGTPIMLNADKLGITPEQLIEKAKADHEADFASFNISFDNYHSTHSEE
                     NRQITTDIYKTLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
                     VCASTYSPMDLINPRSAVSGATPVVKESEHFFFDLPSFEGMLKEWTRSGSLQPEIANK
                     MQEWFESGLQQWDISRDAPYFGFEIPDAENKFFYVWLDAPIGYIASFKNLCDRAGIDF
                     DEFWQKDSTTELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSK
                     SRGTFIQANTYLKHLDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNSDIVNKLVNLA
                     SRNASFIAKRFEGKLAEKLDDEALFNEFTAQAETIANFYENREFNKAIRAIMELTDKA
                     NKYIDDKAPWVIAKEEGKEAELQAVCSMGIELFRVLMSYLKPVLPKLAERAEAFLQSE
                     LRWDNIATPLFGHTVAPFKSLFSRLEKKQIDAVIDETKALFAEANKKTENQTKGTQKV
                     EQQAVEKQENIAVEPISPEITIDDFAKLDLRAAKVLNCEAVPKSDKMLRFELDLGDHQ
                     RQVFSGIKAAYPNPEALIGRFVIVIANLAPRKMSFGMSEGMILSAGTGGANLFLLDAD
                     SGVQPGMQVK"
     gene            9695..10666
                     /gene="menC"
                     /locus_tag="PXD71_000277"
     CDS             9695..10666
                     /gene="menC"
                     /locus_tag="PXD71_000277"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDL6290444.1"
                     /translation="MRQVKLYRYQLPIQTGVVLRKQPLKERQGVLVCLQEQEKVGWGE
                     IAPLPSFSQESLEQAENQAVNWLKAWQKGEPETLEQLFPSVAFGLSCALAELNNELGE
                     QANNQSAVLCYGDLEKLHTAFSQHTNPIGKIKIGLNPEQEGEQANLLLHTFPNLRLRL
                     DANRLWSLEQAVKFAEKIANEHKPRIQFIEEPCQALELSRQFATQTDIAIAWDETVRE
                     ANFLVKKEPNLTAIIIKPTLVGSLEKCVNLIQQAQKQGLVAVISSSLESSLGLTQLAR
                     IAQQYTPNSVPGLDTLNLMQHQLLRDWHDSSLPLVGLESEFVREVVV"
     gene            10911..11612
                     /gene="rsuA"
                     /locus_tag="PXD71_000278"
     CDS             10911..11612
                     /gene="rsuA"
                     /locus_tag="PXD71_000278"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDL6290445.1"
                     /translation="MRLDKFIAENTGLTRSQAAKVLKSGVVTVNGNIEKSGAAKISQE
                     DEIYYEDQKLEWVEEGQYFMLYKPQGYICSHDDGEYPTVFQFFDYPLMTKLHTAGRLD
                     VDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAQRLAEGILLRGEKEP
                     TLPAQLEILDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDERLEEGE
                     FRSLTSEEIASFNGK"
     gene            11602..12783
                     /locus_tag="PXD71_000279"
     CDS             11602..12783
                     /locus_tag="PXD71_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDL6290446.1"
                     /translation="MESRRPSLSFFIILGMMAMLPPLAIDMYLPSFLDIARDLAVSQE
                     KVQTTLAVFTLGFGAGQLFWGPMADSFGRKIIILIGLVGSAIAAYFLTQVQNIETFYL
                     LRLVQGLCAAAPAVVLGALVRDLFERNLFAKLMSVIMIISMLAPLLAPIVGGYIAKYF
                     HWHSIFYVLVAMGILCVVLISWKIPETLAPEKRLPLSFGKTLSNFAILLKHRPTLGYV
                     LIGGLTFAGIFCFLTSGSIVYIGLYGVSQENFGYFFGLNMIVMVTMTAINSKMVVKVG
                     SETMLRIALAMQLLAGIWLALVAIFDLGFWAMAIGIPLYIGMLSTIGSNASAAILEQF
                     PQMAGTANGVAGTARFGVASLVGAGLSHIAVTSAAPMLLAMALCVMLAGVVYYFLCYK
                     R"
     gene            complement(12923..14242)
                     /gene="rlmD"
                     /locus_tag="PXD71_000280"
     CDS             complement(12923..14242)
                     /gene="rlmD"
                     /locus_tag="PXD71_000280"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL6290447.1"
                     /translation="MAIFYSEKSAKKPIKSPACQKATIQSLDYQGLGIAKINGKTWFI
                     ENALPDEQVEFKVLEEKRQYGRGQAVKILKKSANRRMPSCELYGQCGGCQMQHISLEL
                     QRKAKQQALFQRLQKLQTHAIDVQPMIIGNDKHYRRRAKLSIAIQQGKAVIGFRLQNS
                     NQIIPLAHCEVLVEPLANLLPKLKHLIENWQQPKKLGHIELVSADNTIALFFRHLGAL
                     SAQDNHNLRQFATQEQLSLFVMTSENHIEHLCGERPYYQIQGLKLHFSIRDFIQVNAT
                     LNEKMVEKALEWLELSNQDRVLDLFCGMGNFTLPIAERAKSVVGVEGVEPMVQQAREN
                     AVTSGLKNVEFYQTNLDEPFSDKPWAKEPFNKVLLDPARNGALFCLDHLTSLNPERIV
                     YVSCNPATLVRDAEKLLQLGYRLEKAAMIDMFPHTGHLESISLFLKS"
     gene            14327..15652
                     /locus_tag="PXD71_000281"
     CDS             14327..15652
                     /locus_tag="PXD71_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HDL6290448.1"
                     /translation="MISSVWNERYLADKKREQDHRSPFQRDRARLLHSEAFRCLQAKT
                     QIHAVGEDDFYRTRLTHSLEVAQIGSSLREKLLADVEGFQAVCSEQEFAKNMANLTAL
                     LPDHSLVESLCFAHDLGHPPFGHGGEMALNYKMYAFGGFEGNAQSFRIMTQLEPYTET
                     AGMNLTRRTLLGVMKYPALLSQTSPIEPAEMSEARHINLHHFPQSKGIFDDNNHFFEW
                     VLAPLSEKDRRQFCTVQPNQDPKQPNKTQYKSLDCSIMELADDIAYGVHDLEDAIVGG
                     MVTPQSWQNAEKLLAECQSDWVKQRLPEIREKLFSQHRYERKDVIGALVNHFITNVRW
                     KALPEFDEPLLRYNAYLPESVACVLKILKDFVYQYVICDAKTQRVERKGQRILMELFD
                     ILSSDPMRLLPNNVRERWKNAEESKRARVICDYLAGMSDGQAFKLFESL"
     gene            15773..16072
                     /locus_tag="PXD71_000282"
     CDS             15773..16072
                     /locus_tag="PXD71_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290449.1"
                     /translation="MRFLPSEIYQPRGELVKADRQGNGEFEVEYRVSGNDVRGLAKSA
                     IAHAKRKGFHLVESDIHRDDADLKFKRGDQELDIEIEVKGRNRIEYKADLDLDKN"
     gene            16233..19145
                     /gene="rapA"
                     /locus_tag="PXD71_000283"
     CDS             16233..19145
                     /gene="rapA"
                     /locus_tag="PXD71_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDL6290450.1"
                     /translation="MFLVGQRWLSESENNLGLGIVTSVDHRTVTINFPAAEEERVYAI
                     AVAPLTRVQFQAGDRINSIDGWQLEVSEVVENQGAVIYLGKRVDNGEEAVLPEMLLDH
                     KISFSKPQDRLFSAQIDRSDRFALRFNALQHQQAQYQSPLRGLRGIRASLIPHQLHIA
                     KEVGQRLAPRVLLADEVGLGKTIEAGMILQQQLFSGRAERVLVLVPESLQHQWLVEML
                     RRFNLKFSLFDEERCSDFDKQDENGDDVSENPFESEALVITSLNWLETSPQRANQVLA
                     ADWDLLIVDEAHHLEWSETESSIGYQLVERLSQQIPAVLLLTATPEQLGQESHFARLA
                     LLDSDRFYDYAEFVQEQAQYKPVADAVSSLLSDKPLSNDEQNAIAELLSEQDTEPMFR
                     AINSKKSDENDRLQARQELISELIDRHGTSRVLFRNTRQGVKGFPHREYHQISLEMPK
                     QYQNALKVMGMMGSVSQSDLLYPEYLFQRMNPNAKWAEFDPRIDWLITFLKNHRDEKV
                     LVICKQADTAIALEQVLREREAIRSAVFHEKMSIVERDKAAAYFAQQEEGAQVLISSS
                     IGSEGRNFQFAKDLVLFNLPDNPDLLEQSIGRLDRIGQKNDIQIYVPCFNLSAQQMLA
                     EWYHKGLNAFEETCPMGTAIFRELGEELQQFLQKPELSAQFDDFVAKTYKRQQYLKAE
                     LEKGQDRLLELNSNGGEAAQQLANDIAKEDNNPQLVNFALSLFDVIGVEQEDLGEQSI
                     VISPTGHMLVPDFPGLSEDGTTITFNRELALMREEVEFLTWDHPMIRNGIDLITSGDI
                     GKSAISLLVNNKLPAGTLLLEALYVVEAQAPKGLNLTRFLPPTPIRVLVDNKGNNIAP
                     QVSFAGLERQLKPVNKQMANKIAKMARADIEKLIKASEKAVEPQVQQVIETAKFEANS
                     KLKAELHRLESLKMVNKNIRADEVEALEQQLNASLAQLETANYRLDSLRVIVSNKA"
     gene            19272..19568
                     /locus_tag="PXD71_000284"
     CDS             19272..19568
                     /locus_tag="PXD71_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL6290451.1"
                     /translation="MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKV
                     VLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRLISVRRSRKKEISYYEKYH"
     gene            19552..19794
                     /locus_tag="PXD71_000285"
     CDS             19552..19794
                     /locus_tag="PXD71_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDL6290452.1"
                     /translation="MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFP
                     NWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR"
     gene            19806..20474
                     /gene="rluA"
                     /locus_tag="PXD71_000286"
     CDS             19806..20474
                     /gene="rluA"
                     /locus_tag="PXD71_000286"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDL6290453.1"
                     /translation="MALIEYNPPLEPFLDEVYRDNHILVINKPSGLLSVPGNRPEYYD
                     SAMTRVQQKYGFTEPAHRLDMATSGILLFALSKAAEKELKRQFREREPKKHYIALLWG
                     KLGEKVGDKGEMNFPLICDWENRPRQKICYERGKKAITHYEVLAHYPNNIARVKLTPI
                     TGRSHQLRVHSLALGHPIIGDKFYANPLAKSLSPRLCLHAESLTITHPITRELMTFTA
                     EPTF"
     gene            complement(20452..21027)
                     /gene="ampD"
                     /locus_tag="PXD71_000287"
     CDS             complement(20452..21027)
                     /gene="ampD"
                     /locus_tag="PXD71_000287"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDL6290454.1"
                     /translation="MPNLKIEQGCLTGQKQVPSPHFSQRPDQEDISLLVIHYISLPPE
                     EFGGNFIDLFFQGKLEPTAHPYFEEIKDLRVSAHCLINREGEITQYVNFNDMAWHAGV
                     SCFEGREKCNEYSIGIELEGSNNQPFTQAQYQSLANLTKAIQQSYPQITDERITGHCH
                     IAPERKIDPGQYFDWAYYKHLLNEAKKSAQQ"
     gene            21087..21929
                     /locus_tag="PXD71_000288"
     CDS             21087..21929
                     /locus_tag="PXD71_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HDL6290455.1"
                     /translation="MLSYRHSFHAGNHADVLKHIVQLLILRALKEKEKGFLYLDTHSG
                     VGRYSLLSFESEKTGEYLEGVARLWERDDLPEEVAFYIDELKKLNRGGKLKYYAGSPL
                     LAVQQLREQDRAILTELHPNDFPLLRQEFAKAKNVQTKRENGFQQLKSALPPKERRGF
                     VLIDPPYELKEDYELVVKAIEEGYKRFATGVYAIWYPVVLRQHTKRIVRGLMDTGIRK
                     ILQIELAVRPDSDQRGMTASGMIVINPPWQLETQMKKILPYLTEVLVPEGTGSWKVEW
                     ITPE"
     gene            22052..22693
                     /gene="pyrE"
                     /locus_tag="PXD71_000289"
     CDS             22052..22693
                     /gene="pyrE"
                     /locus_tag="PXD71_000289"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL6290456.1"
                     /translation="MQQYKHDFIEFALSRNVLKFGEFTLKSGRISPYFFNAGLFNTGR
                     DLAKLGEYYAKAIQASGIEYDVLFGPAYKGIPIATTVAVALANLFDVDKPCCFNRKEA
                     KDHGEGGNLIGSPLTGKILLVDDVITAGTAIRESMEIINANNAQLAGVLIALNRKEKG
                     KGELSAIQEVERDYGCQVFSIVDFDDLVAFIETSPQYAPYLPKMREYREKYGV"
     gene            22867..23956
                     /locus_tag="PXD71_000290"
                     /pseudo
     CDS             22867..23956
                     /locus_tag="PXD71_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     gene            complement(24021..25211)
                     /gene="metC"
                     /locus_tag="PXD71_000291"
     CDS             complement(24021..25211)
                     /gene="metC"
                     /locus_tag="PXD71_000291"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDL6290457.1"
                     /translation="MSNYKNLSTKLVHTGRKKRNTQGAVNPVIQRASSLVFETLEEKK
                     HATQNRYKGALFYGRRGTLTHFSLQEAMCELEGGAGCYLYPCGAAAVTNAILAFVEQG
                     NHVLMTGAAYEPTQEFCNALLKRMGVSTTYYDPMIGEGLKALVQPNTKVLFLESPSSL
                     TMEVPDIPTLVRVAREINPEIVIMIDNTWSAGVLFPALEYGIDISIQAGTKYLVGHSD
                     VMIGTAVSNARCWDKLREGSYLMGQMVDADSAYITARGLRTLGIRLKQHEQSSIAVAN
                     WLAQRPEVKAVYHPALESCPGHEFFKRDFLGSSGLFSFELCEKLSNDKLANFLEHFEL
                     FSMAYSWGGFESLILANQPEEIAKIRPAIERKLTGTLIRIHVGLEAVEDLIADLEQGF
                     NRIR"
     gene            complement(25270..25809)
                     /gene="dsbB"
                     /locus_tag="PXD71_000292"
     CDS             complement(25270..25809)
                     /gene="dsbB"
                     /locus_tag="PXD71_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDL6290458.1"
                     /translation="MLYFFKNLSQSRSAWFLLMLSCLILEGTALYFQHGMGLVPCVMC
                     IYERVALFGILFAGILGFLGNRYAIIRYLAILIWLGSAFKGLVLSIKHHDYQVNPSPW
                     NQCEFKVNFPQTLPLDQWLPQVFAPGPVNCSQSQWEMFGFSMAQWLIFTFAVYLIVAV
                     IVLISQFKRSRQRNNFMFR"
     gene            complement(25872..27413)
                     /gene="nhaB"
                     /locus_tag="PXD71_000293"
     CDS             complement(25872..27413)
                     /gene="nhaB"
                     /locus_tag="PXD71_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HDL6290459.1"
                     /translation="MSSIDALFKSFLGKSPEWYKLCILAFLIINPLIYFFINPFVAGW
                     FLVAEFIFTLSMALKCYPLQPGGLLAIEAVVIGMTNPHHIKEEIMANFDVILLLMFMV
                     AGIYFMKQLLLYIFTKLLVAIHSKKILSLSFCLSAAFLSAFLDALTVIAVIISVGTGF
                     YGVYHKVASGNNFEDSTDITNDEKIISNKEILEQFRGFLRSLLMHAGIGSALGGVMTM
                     VGEPQNLIIASQAEWGFIEFLLRALPVSLPVLICGIATCFLVEKYKLFGYGDRLPSKV
                     WGVLARYSQLKEQKMTKQDRLKMLVQAIAGIWLIIGLALHLADVGIIGLTIIIICTAF
                     CGITDEHTLGKAFQESMPFTALLIVFFSVVAVIIDLKLFEPIIAFVLSAEPHSQLSLF
                     YIFNGLLSMISDNVFVGTVYINEAKTALDAGIIDREQFDLIAVAINTGTNLPSVATPN
                     GQAAFLFLLTSSFAPLIKLSYGRMVYMALPYTIVLSIVGFLGLEFLLPSMTEVMINWG
                     WFITR"
     gene            27570..28295
                     /gene="fadR"
                     /locus_tag="PXD71_000294"
     CDS             27570..28295
                     /gene="fadR"
                     /locus_tag="PXD71_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDL6290460.1"
                     /translation="MGHSDILKAQSPAALAEEYIVKSIWNNHFPAGSDLPAERELAER
                     IGVTRTTLREVLQRLAREGWLSIQHGKPTRVNDIWETSGPNIIGTIIKLDKSYLPVVI
                     ANVVSLRTRMAEAYIPEAIYLAPEESAKLFDGLDELENTAAAYAEFDYKLFRQFTFLA
                     NKPVYALILNSFKSMYHQVAKIFFADENNRQLTLSFYRSLLCTCLAKDSEKAAACMAK
                     NRQSSGEIWQKLSANIPESFGQL"
     gene            28310..29380
                     /locus_tag="PXD71_000295"
     CDS             28310..29380
                     /locus_tag="PXD71_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL6290461.1"
                     /translation="MNEIIFSLEPQDNARLQSLCGAFDEHLALIEKSFNLIIARTGFN
                     FTIQSEDDNHYSATLVQNSVKLIKQLYNETAPIKGKIKELDLEDIHLAIQESRMLLQN
                     QWQAGSQGEIAIKTKRGVIKPRSEHQQAYLRNILSHDISFGIGPAGTGKTFLAVAAAV
                     ESLERQEIRRILLTRPAVEAGEKLGFLPGDLGQKIEPYLRPLYDALFEMLGFEKAQKL
                     MERGVIEIAPLAYMRGRTLNDAFIILDESQNTTTEQMKMFLTRIGFNSKAVITGDITQ
                     VDLPRSQKSGLKHAMEVLKEVPELSFNFFDSKDIVRHPVVAKIVQAYDAWEAVDEERK
                     EQRKLEKLALEQEKILAQAEML"
     gene            complement(29506..31512)
                     /gene="tkt"
                     /locus_tag="PXD71_000296"
     CDS             complement(29506..31512)
                     /gene="tkt"
                     /locus_tag="PXD71_000296"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL6290462.1"
                     /translation="MTERKVLANAIRFLSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPTNPKWADRDRFVLSNGHGSMLIYSLLHLTGYDLSIEDLKQFRQLHSKTPGHPE
                     YGYAPGVETTTGPLGQGITNAVGIAIAEKTLAAQFNREGHKIVDHYTYAFLGDGCLME
                     GISHEACSLAGTLGLGKLIAFYDDNNISIDGHVDGWFTDDTAQRFESYGWQVIRNVDG
                     HNPEQIKFAIENAQAETERPTLIICKTIIGYGSPNKSNSHDCHGAPLGDAEIQAAREF
                     LGWEHAPFVIPAEIYTEWDAKAKGTLAEKDWNAKFAAYEAAYPELAAEFKRRYAGELP
                     ANWEAESKAFIEKLQANPATIASRKASQNAIEAYAPILPEFLGGSADLASSNLTLWSG
                     SKPIRAEHNVDGNYINYGVREFGMSAIMNGIALHGGFIPYGATFLMFYEYAHNAVRMA
                     ALMKQRALFVYTHDSIGLGEDGPTHQPVEQTASLRQIPNLETWRPADQVESAVAWKAA
                     VERKDGPTALIFTRQNLAQQQRTAEQLANISRGGYILRECCEKGSCPDLILIATGSEV
                     DLAMKAADVLDAEGVKVRVVSMPSTNVFDKQDEAYRESVLPRSVTKRVAIEAQLADFW
                     YKYVGFDGRIVGMNSFGESAPADQLFKLFGFTVENVVAKAKEIL"
BASE COUNT         9165 a         6255 c         7067 g         9171 t
ORIGIN      
        1 aatcttgttg tgttactgtt tcccatcgct aatatagcat agcttgttag ataattcttt
       61 aaaggagatt actatggcta tgccatctca acgtttttct cttacttggg tactaaattt
      121 atttggtact gcggtaggag ccggcgtttt atttttgcca attaatgcag gtatgggtgg
      181 gttctaccca cttattatta tgactttgtt agttggacca atgacatatt tagctcaccg
      241 tggcttaaca cgttttgtgc tttcttcaaa atacaaaggg agcgatatta cggctgttgt
      301 tcgtgaacat tttggtgaac aggccggtaa attgatcacg ctactgtact tctttgccat
      361 ttttccgatt ttactgattt atggtgtagg tattaccaat accgtgagtt cttttatgga
      421 gaatcagctt catatcgcgc caccatctag ggctgtatta tcgtttatgc taatagctgc
      481 tatgatcgga gtaatgttat taagtgagca agtcatgtta aaaatcacaa cttgtcttgt
      541 gtatccatta gtgttgattt tattgggctt atctatttat ttaattccac agtggaatac
      601 agctgcctta cagcaaatgc caactacagg agacttcctt actacacttt ggctgactat
      661 tcctgtttta gtctttgctt ttaaccattc accggctatt tcttcttttg cactttctca
      721 acagaaatat taccaagatg acaagaaggc tgaaattgaa tcagctaaag tattacgttc
      781 taccgccttt atcttggtgt tatttgtgat gttctttgta tttagctgtg tattaacttt
      841 gacacctgaa gaattagcac aagctaaagt gcaaaatatt tcgattcttt cttaccttgc
      901 gaataaattt gataatccga ttatttccta ttttggaccg ttggttgctt ttttggcgat
      961 tggtagctct ttctttgggc attatttagg tgcgcgcgag gggttagaag gattggttaa
     1021 tcaaatgcgt aaagagccga ttgatccaag caaattccgc aaaattaccg ctattacttt
     1081 tttgatcatt ttatggattg ttgcgactat caatccaagc attttaggct tcattgaaag
     1141 ccttggtggg ccgattattg cgatgatctt gtttattatg cctgtctatg ccgtatataa
     1201 agtaccggca cttgcacgtt ttaaaggtga atttggccat ttatttgtgc ttgttatggg
     1261 ttgtattgct atttcagcaa tagtatatgg gttactgtaa tctttctaaa atcaatgtag
     1321 aatacgccct tttattttct atgtgaattt gtgtttatga ttagtgtatt tgatatgttt
     1381 aaagtaggga ttgggccttc cagttcccat actgcggggc cgatgaaagc ggcaaaggaa
     1441 tttgtcgatg aattacaaaa tcgcaaattt ttaaacaaaa ttgaccgctt gcgggtagat
     1501 gtctatggtt cattatcact caccgggcga ggacataata cggatattgc catcataatg
     1561 ggattaatgg ggtatttgcc tcataatgtg gatattgaac gtattgagca gacgattgaa
     1621 aatctaaaac aaaataagca acttatcatt gctgaaagca gtgttgagcc taaagtgatt
     1681 gcgttcgatt tttatgagga tatgccattt catcgagatt tcttacctcg gcacgaaaat
     1741 ggtgtgaaat ttagtgcctt gagcggtgaa gagttgctat ttcaaaacac ctattattct
     1801 attggtggtg gctttattgt tgatgaagct aactttgagc aagttaaaaa ttcagatctt
     1861 tctgttccat tcccatatca gaatgcagca gatttacttg cacattgtaa gaataacggg
     1921 ttaccgcttt cttctttagt ctggaaaaat gagcttgctt tacgcagtag agtagaaatt
     1981 tcggattatt tggcaatgat ttggaataca atggaagcct gtattgagcg tggtttgaat
     2041 acggaagggt tactacccgg gcctctgaaa gtggtacgcc gtgccagtgg gctacgcaga
     2101 atgttagagg caaacagtaa attcaatacc gacccaatgc aaattatcga ttgggtgaat
     2161 atgtttgccc ttgccgtgaa tgaagaaaat gcggcaggtg ggcgagtagt caccgcacct
     2221 accaatggag cctgtggtat tgttccggct gtgttggcgt actaccaaaa atttattgca
     2281 cagcttaatc aagatactat tgagcgttat ttattgacta ccagtgtgat tggttcgtta
     2341 tacaaaatga atgcctctat ttccggtgct gaagtaggtt gccaagggga agttggtgtt
     2401 gcttgttcaa tggcggcagc aggtttaacg gaaattatgg gtggcagtgc tgaacaggtg
     2461 tgtatggctg cagaaattgc aatggagcac aatttaggcc taacttgtga ccctgttggc
     2521 ggacaagtgc aagtgccttg tattgaaaga aatgcaatcg cttcagtcaa agcgattaat
     2581 gccagcagaa tggcactacg ccgtacttct tcgcctcagg taagtttaga taaagtcatt
     2641 gaaacaatgt acgaaaccgg caaagatatg aatgcaaaat accgtgaaac ctcaaccggt
     2701 ggcttagcaa tcaaaatttt accttgtgat taattaatga aaaaggctac cttatcaaaa
     2761 taaggtagcc ttttagtttg tagaacatta atgacggaaa tgcctcattc cggttagtac
     2821 cactgccata ccgtgttcgt tggcggcatc aatcacttct tgatcacgca ttgaaccacc
     2881 tgggtgaatc acacaagtaa tccctacttt tgcagcagcg tcaataccat cacggaacgg
     2941 gaagaacgca tcggaagcca ttacacaacc cacaacttgt aagccctcgt cttccgcttt
     3001 aatacctgca atttttgcgg aataaacacg gctcatctgc cctgcaccga taccgatagt
     3061 ttgcccgtct ttggcataca caatcgcatt cgatttcaca tatttcgcca ctttccagca
     3121 gaacaatagg tcttttagct cagcttcagt tggtttacgc tctgaaacca cctttaaatc
     3181 gtcaatagat acagagcctt ggtcggcatc ttgcactaat aatccgccgt ttacccgttt
     3241 gaaatctaag cgttttacag cttctgagaa ctcgccacat tcaagcacac gcacgttttt
     3301 cttagtggct aacacttctt tggcagaagc agaaacggtt ggtgcaataa tcacttcaac
     3361 aaattgacgg tctgcgatgg tttgagcagt ttcgccgtct aattcacggt taaaggcaat
     3421 aatgccaccg aaagcagaag ttggatcggt ttggtaagca cggttgtagg catctaaaat
     3481 atttttaccc aaagcaacac cacaagggtt agcgtgtttt acaatcacac aagcaggctc
     3541 gctaaattct ttcacgcatt caagagccgc atcggtatct gcaatattat tatacgaaag
     3601 ggctttacct tgtaactgtt tggcggtgga aacagaggct tcttttaact cattttcgac
     3661 atagaatgca gcgttttgat gcgaattttc gccataacgc atcgtttgtt tacgcacaaa
     3721 attgaggttt aaggtgcgag ggaattgtcc acttggttgg gttaaatctt cttcctcagc
     3781 cccttgataa ggcggcactt tttgcccgaa atagttggca atcattgaat cgtattgtgc
     3841 ggtatgctca aacgctttaa tagcaagatt aaaacgggtt tctaaagtta aactgttttg
     3901 atgttgatcc atttcggcaa gaatagcatc aaaatcattg ttattgacca caatcgccac
     3961 atctttatga tttttcgctg cagaacgcac cattgtcgga ccaccgatat caatattttc
     4021 caccgcatct tcaaggctac aatttggatt ggcaacggtt tgagcaaaag ggtagaggtt
     4081 tactaccacc atatcaattc gttcaatgcc gtgttggttc atcacttcat catcagtacc
     4141 acggcgacct agaattccgc cgtgcacttt cgggtgcagg gtttttacac gcccgtccat
     4201 catttcagga aagccggtgt aatctgatac ttctgttact agcaaaccgg cttcagccag
     4261 taattttgcg gttccgcccg tagaaagtaa ttttacccca cgagccgata gcccacgagc
     4321 aaattcgaca atgccttttt tatcagacac acttaataac gcctgttgaa tcgccattct
     4381 taaattcctt aaaatgagtg ttaacgtgaa aataaacggc aaagattata acgcaaacgt
     4441 ttgctttttt catctacaag tggtcgattt tgcaaatttt tctgcaaaat cgaccgcttg
     4501 cactcgtgat taaagcgggt agaatagagg aaaaaaatcg gaggaactat gattaaccac
     4561 aatttagcag atttcgtatt tgagtatttt accgaaaaca acctaaaagc aggcacacta
     4621 cgttatcgct acataaaaga gaacgtgatt gatgcttaca gtacccgagt agatgatgcc
     4681 tttcaaggta aaggtattgc aggagagctt tacaatgctt taattgcgtt tgcgcaagaa
     4741 aaagggctga aaatcaagcc aagttgtagt tatattgagg taaaaatgca acgaagtcat
     4801 cgagatttaa ttgcttagag aggagcaagc ggtcgtaaaa tttcaaaaat ttacgaccgc
     4861 tgtcttattc aattctcaaa aatccatcac acaaaatata agccctattc attaaacaac
     4921 gccatataaa acttattttc aaatgtatta agcggagccc gtttggtttt gctttcgagc
     4981 tcgccggtgg aatagccgtt aataaattgc acaaaagcga tgcgttcgcc acgggcgttt
     5041 ttcataaagc cggcaaggtt gtaaacccct ttcagtgagc cggttttggc aatcagattt
     5101 ttggcaagcg gttctgttga aatagagcct ctgccggaaa tagtaccgtc cacgcctgca
     5161 atcgggaagg tttcaaacag ttttaagctt tcttcgtttt gagcgatata ttcaagggtc
     5221 tccagcattg ttcggctgct gacttgatta tgacgggaaa gccctgagcc atcagccacc
     5281 acgctgtttt taaaatcaat attggctttg gtttttaata actggcgaat cacataacta
     5341 cctaattgga acgatgcggg gcggttgtgt tgcttgttgg caacggttct aaacagggca
     5401 tcggcaattt ggttatccga ttttttcatc atttttttca ataataccgg caacggctcg
     5461 gaataatgct cggcaagtag cgtgccgttt tgggcagtaa gtggctcttt tacttgcccg
     5521 ttaaaggcaa ttttcaggct ttttaactgg gctttaagca tatttgcccc gtaattagtt
     5581 gggtcttgca ccgaaaaact taagccgaac ggttgagact ggcgagccat acagcctttg
     5641 atttggtagc ggttgttatc gtgaaccacc acatcaagct ggcaaaacgg agcttctttc
     5701 ggctcgacaa tataagcaga gctaaacacc tgcaccggat aagcggacgg cacattcact
     5761 ttagcaaatt cgccaatggg ttggtcggca tttaaggtga cataaaaaca gttgtgatcc
     5821 acattaattg ccgccggtgg ggcgttaaag cacatcgtca agtcgttcca aatccaaccg
     5881 gaggctttgt cgtggctggc gaaaaccgac gggtctagaa ttaaatcccc ctcaatttta
     5941 ttgatacctt gttgctttaa tttgctcatt aattgataaa tttgaccgct tgtaagctca
     6001 gggtcgccac taaaacgggc aattaaattg ccttttaaca ctccgttttc tacttttccg
     6061 ttggttaaaa gggcagtttg aaagcggaaa tcatcgttta aagttaattt tgctgccagt
     6121 gccgtaaaga ctttttgggt actggcaggt agcataaaaa tatcactttg gtattgggtg
     6181 atgatttggt tagtgtctaa atttttagca ataaaactaa cagaagtacc ggcaggcaag
     6241 gtatttaata attcttcggt attaatttca gcctgtgtaa tgcttggaat cagaagtgta
     6301 gataagataa atttatttaa aaaagcacga gtgaaatttt taaaaatcat aataaaaggg
     6361 gagttgaaat ttttagtgag aaattgaata atagcccttt cgttttaatt cgtaaaccga
     6421 taatttttag taaggaaata gatttaatga aacaaattcc tatgacggtg cgtggtgcag
     6481 agcaactgcg tgaagaattg gattttttaa aaaatgtgcg tcgcccacaa attattgatg
     6541 cgattgcaga agcccgtgaa cacggtgatt taaaagagaa cgcggagtat cacgcagccc
     6601 gtgaacaaca aggtttctgt gaggggcgta ttcaagaaat tgaaggcaaa ttaggcaatg
     6661 ctcaaattat tgacgtcact aaaatggcaa acaacggcaa agtgattttt ggggcaacgg
     6721 tgcagttagt gaatgctgat acagatgaag aagtcactta ccgcattgtg ggtgatgatg
     6781 aagccaatat taaagagggc ttaatttcag ttaattcccc gattgctcgt ggtttaatcg
     6841 gcaaagaggt ggacgattca gtcagcatta ccacaccggg tggcaaagtt gagtttgaca
     6901 ttgttggcgt agaatatatt taatctacgc tagggcgaat tacaatagtg gggcgaaaaa
     6961 attttcgccc ctactttatt aaacaatctc actcggtaca aagaagtagg caatttcacg
     7021 ttttgccgac tcttcggaat ctgaaccgtg taccgaattt tcacgcatac ttaaggcata
     7081 taaatcgcga atggtgcctt tttcacggtt atccggattg gttgccccca ttaatgcacg
     7141 ataatggcta attgcatttt ccccttccag tacagaaacg acaatagatc ctgatgtcat
     7201 aaattccact aacggctcaa agaaatcttt gcctttgtgt tccgcataaa atccttccgc
     7261 ttgctcacgg gtaagatgta ctttttttag agctttgata gtcagcccgt tttcttccaa
     7321 ttgagccaaa attttgccga ttaaattacg gcgtgtagca tccggcttaa taatagaaag
     7381 cgtttgttga atcatcatct tctcctattt aacttgcatt cccggctgaa caccgctgtc
     7441 tgcatctaat aagaacaggt tcgcaccgcc tgtgccggca gataaaatca ttccctccga
     7501 cataccgaac gacattttgc gtggagcaag gttggcaatt acaatcacaa aacgcccgat
     7561 aagtgcttcc ggatttgggt aggccgcctt aatgcctgag aatacttggc gttggtggtc
     7621 gcctaaatcc agctcaaaac gtaacatttt gtctgatttt ggcacagcct cgcagttcaa
     7681 tactttggct gcacgtaaat ctaatttcgc gaaatcatca atcgtaattt cagggctgat
     7741 tggctcaact gcaatatttt cttgtttttc gaccgcttgt tgttccactt tttgtgttcc
     7801 tttggtttgg ttttcggttt tcttattcgc ttcggcaaat aaggctttgg tttcatcaat
     7861 cactgcatca atctgttttt tctcaaggcg tgagaatagc gatttaaacg gtgccacggt
     7921 atgcccgaat aatggggtag cgatattatc ccaacgcaat tcgctttgta agaatgcttc
     7981 ggcacgttct gccagttttg gtaacaccgg ttttaggtag ctcatcagca cacggaataa
     8041 ctcaatgccc attgaacaaa ccgcttgtaa ttcagcctct ttgccttctt ctttggcgat
     8101 gacccaaggg gctttgtcgt caatgtattt attggcttta tcggttaatt ccataatggc
     8161 acgaatagct ttattaaatt cacggttttc atagaaattc gctatggttt ctgcttgagc
     8221 ggtaaattca ttaaataacg cttcgtcatc taatttttca gctaatttgc cctcaaagcg
     8281 tttagcgata aaactggcgt tgcgagaagc aagattcact aatttgttta cgatatcgct
     8341 gtttacacgt tgcacgaagt cgtctaaatt aaaatctaaa tcttcaatgc gatcgtttaa
     8401 ttttgccgca tagtaatagc gtaagcattc cggatcgaga tgttttaaat aggtgttggc
     8461 ttgaatgaat gtaccacgag atttcgacat tttcgccccg tccactgtca cataaccgtg
     8521 agcaaacaca ttggtcggtt tgcggtagcc gctgccttcc aacattgccg gccaaaacag
     8581 gctgtggaag taaacaatgt ctttaccgat gaagtgataa agctcggtgg tggaatcttt
     8641 ttgccaaaac tcatcaaaat caatgcctgc acggtcgcat aaatttttaa acgatgctat
     8701 ataaccaata ggggcatcta accaaacgta gaagaatttg ttttctgcgt ccggaatttc
     8761 aaaaccgaaa taaggggcat cacggctgat gtcccattgc tgtaaaccac tttcaaacca
     8821 ttcttgcatt ttattggcaa tttcaggttg gagtgagccg gaacgagtcc actctttcaa
     8881 cattccctca aagcttggca ggtcaaaaaa gaaatgttct gattctttta ccaccggagt
     8941 tgcaccggaa actgccgagc gagggttgat taaatccatc gggctatagg tagaggcaca
     9001 tacttcacag ttatcgccat attgatcttc ggctttacat ttcgggcagg tccctttcac
     9061 aaagcgatcc ggcaagaaca tatttttttc tgggtcgaac agttgtgaaa tcactttgct
     9121 tttaataaaa ccgttcgctt ttaaggtttt ataaatatcg gtagtgattt ggcggttctc
     9181 ttcgctgtgg gtggaatggt aattatcaaa gctgatatta aaactggcaa aatccgcttc
     9241 gtgatcggct ttggcttttt cgattaactg ctcaggggta atgcctaatt tatcggcatt
     9301 aagcataata ggcgtgccgt gggcatcatc agcacagaca aagtggactt cattgccacg
     9361 catacgttga aaacgcaccc aaatatctgc ttgaatatgt tcaagcatat gacctaaatg
     9421 aattgcaccg ttagcatagg gcaaggcaca agtaacaagc atttttcgtt tttggttagc
     9481 cattcttttt ctcattaaaa ttagggtaaa aattgccgtc tattttagcc caaaagtggc
     9541 aggtttacca gttggcgtga atttattaga gctttgggct aacaagcggt agaatatgat
     9601 gcgctagagg cgaaacattt ttcgcctcaa tttcccaaat gatgatgtgg gcgaaagatg
     9661 tttcgcctct aaacctattt tggataaaaa tagtatgcga caagtaaagt tataccgcta
     9721 ccaactcccc attcaaaccg gtgtagtgtt acgcaagcaa cctttgaaag aaaggcaagg
     9781 ggtgttggtg tgtttgcaag aacaggagaa agtcggctgg ggcgaaattg caccgcttcc
     9841 aagttttagc caagagagcc tagaacaagc tgaaaaccaa gctgtgaatt ggttaaaggc
     9901 gtggcagaaa ggagagcctg aaacgttaga gcagcttttc ccgtctgttg cctttgggct
     9961 aagctgtgcg ttggctgaac tgaataatga attaggcgag caagcgaata accaatcggc
    10021 ggtgctgtgc tatggcgatt tggaaaagtt acacacggca tttagccaac atacgaaccc
    10081 aatcggtaaa attaaaatcg gtctcaatcc tgaacaggaa ggcgagcagg caaatttgct
    10141 gttgcatact tttccgaatt tacgcctaag gcttgatgcc aaccgtcttt ggagcttgga
    10201 acaagcggtc aaatttgctg aaaaaattgc aaatgaacat aaaccccgta ttcaatttat
    10261 cgaagaacct tgccaagcgc tcgaactttc ccgccaattt gccacacaaa ccgacattgc
    10321 gattgcgtgg gacgaaaccg tgagagaggc gaattttttg gtaaaaaaag agccaaattt
    10381 gaccgctatt atcattaaac ctacgctggt tggttcgctt gaaaaatgcg tgaatttaat
    10441 tcaacaagca cagaagcaag gattagtagc ggtgattagt tcaagtcttg agagcagttt
    10501 gggcttaacc caactggctc gtatcgccca acaatacacc ccaaacagtg tgccggggtt
    10561 ggatacgtta aatttaatgc aacaccaatt attgcgagat tggcacgact ctagcttgcc
    10621 gttggtcggg ctagaaagcg aatttgttag ggaagttgtg gtataatctc ccaattaaac
    10681 actctctccc cttgtgggag agagaccgct tatgagcgtt cagcgaaata agcagagaga
    10741 ggggaagcgg tcatttttct aaaaaagttt gcacatttcc cctctccctg atttttcttc
    10801 tggacgaaga cgtatttccc ccctctccct gatttttctt ctagacgaag aaaaatctgt
    10861 ccctctccca cgaggggaga gggtaaagta atttatggta taaaaatagt atgcgtttag
    10921 acaaatttat cgccgaaaat accggtttga cccgttcaca agcggctaaa gtgctgaaaa
    10981 gtggtgtggt gacggtaaac ggcaatattg aaaaaagtgg tgcggcaaaa atcagtcaag
    11041 aagatgaaat ttattacgaa gatcagaaat tagagtgggt ggaagagggg cagtatttta
    11101 tgctctataa gccacagggc tatatttgtt cacacgatga tggtgagtac ccaacggtgt
    11161 ttcaattctt tgattatccg ttaatgacta aattgcatac cgcagggcgg ttagatgtgg
    11221 ataccacagg gctggtgctg ttaaccgatg acggcaaatg gtcgcaccgt attacctcgc
    11281 ccaagcacca ttgtgaaaag acatacttag tgacgcttgc tgatccggtg gaagattttt
    11341 atgcacagcg attggcagaa ggaattttat tgcgtggcga gaaagagcca accttgccgg
    11401 ctcaacttga aattttagac gattacaatg tgaatttaac tattagtgag gggcgttatc
    11461 atcaagttaa gcgaatgttt gccgcccttg ggaataaagt cgaggcattg caccgctggc
    11521 gaattggcga tgtggtgtta gatgaaaggt tggaagaagg tgaattccgc tcgctcactt
    11581 cagaggagat agccagtttt aatggaaagt agaagaccaa gcctgagctt tttcatcata
    11641 ttaggtatga tggcaatgtt gccaccatta gccattgata tgtatttgcc ctcattttta
    11701 gacattgccc gtgacttggc ggtttcgcaa gaaaaagtgc aaaccacctt ggcggtattt
    11761 accctaggtt ttggggcagg gcaactattc tggggaccga tggcagacag ctttgggcgg
    11821 aaaatcatta ttttaatcgg attagtcggc tcggcgattg ccgcttattt tctgacccaa
    11881 gttcagaata ttgaaacatt ttacctgcta aggttagtac agggcttatg tgcggcagcc
    11941 ccggcggtgg tgctgggagc attagtgaga gatctgtttg agcgtaattt atttgccaaa
    12001 ttgatgtcgg ttattatgat tatttctatg cttgccccat tgcttgcacc gattgttggc
    12061 ggttatattg ccaaatattt ccactggcac tcaattttct atgtgttagt ggcgatgggg
    12121 attttgtgcg tggtattaat tagttggaaa atccctgaaa cattagcccc cgaaaaacgc
    12181 ctgccgttaa gttttggcaa aactttaagc aattttgcca tcttactgaa acataggcct
    12241 actcttggtt atgtgctgat tggcggctta acctttgcag gtattttctg ttttttaacc
    12301 tcaggctcga ttgtctatat tgggctttat ggcgtgtcgc aagaaaattt cggctatttc
    12361 ttcgggctga atatgattgt gatggtgacg atgacggcga ttaacagcaa aatggtagtg
    12421 aaagtcggct ctgaaacgat gttacgaatt gctttagcta tgcaactgtt agccggcatt
    12481 tggctggctt tagtggcgat ttttgattta ggcttctggg caatggcaat cggtattccg
    12541 ctttatatcg gaatgctttc caccattggt agtaatgcca gtgccgccat tttagagcaa
    12601 tttccacaaa tggcaggcac ggctaacgga gtagcaggta cggctcgttt tggtgttgct
    12661 tcccttgttg gagcagggct ttcacatatt gctgtaacca gtgctgcccc aatgcttttg
    12721 gcgatggcat tgtgcgttat gttagccggt gtggtgtatt attttctgtg ttataagcga
    12781 taactaggtt ggggttagct tgcgaaaacc caacaaattt ctacaagcgg ttaataatag
    12841 tacatttttt gcaaattttt aggcagaatt aaccgcttgt gtggtacgag cgtggacgct
    12901 cgcaccatcg gggcaatatc tactaagatt ttaaaaacag cgaaatagat tccaaatgcc
    12961 cggtgtgggg gaacatatca atcattgccg ctttttctag cctgtagcct agttgcaaca
    13021 gtttttcggc atctcgcacc agtgttgccg gattgcaaga gacataaaca atccgctcag
    13081 gattgagtga tgtcaagtga tctaggcaaa atagtgctcc gttgcgtgcc gggtcgagta
    13141 gtaccttgtt aaacggctct tttgcccaag gtttgtcaga aaacggctca tctaaattgg
    13201 tttggtaaaa ttccacattt tttagaccgc ttgtcaccgc attctctcgg gcttgttgca
    13261 ccattggttc taccccttcc acaccgacca ccgatttcgc tcgctcagca ataggcaagg
    13321 taaaattccc cattccacag aagagatcta acactcgatc ttggttggat aattccagcc
    13381 actcaagggc tttttccacc attttttcat tgagcgtggc attcacctga ataaaatccc
    13441 gaattgaaaa atgcaacttt agaccctgaa tttgataata cgggcgttca ccgcacagat
    13501 gctcaatgtg attttcgcta gtcatcacaa aaagagataa ttgctcttgt gtggcgaatt
    13561 gacgcaaatt gtggttatct tgcgccgata atgcccctaa atggcggaaa aatagggcaa
    13621 tggtattgtc cgcactgacc agctcaatat gccccaattt tttcggctgt tgccagtttt
    13681 caatcaggtg ttttaatttt gggagcaaat tggcaagcgg ttcaaccagc acttcacagt
    13741 gtgcaagcgg tataatttgg ttggaatttt gcaaacgaaa gccgattacc gccttgcctt
    13801 gctggatcgc aatgcttaat tttgcccgac ggcggtagtg tttatcgttg ccgataatca
    13861 tcggctgaac atcaatagcg tgggtttgca atttctgtaa gcgttggaat aaagcctgtt
    13921 gttttgcctt acgttgcagt tcaagcgaaa tatgctgcat ttggcagcca ccgcattgcc
    13981 cgtaaagttc gcagcttggc attcgccgat ttgcagattt tttaagaatt ttaaccgctt
    14041 gtccacgtcc gtattggcgt ttctcttcca acactttaaa ttccacctgc tcatcaggca
    14101 gggcattttc gataaaccaa gttttgccgt tgattttggc aatccctaag ccttgataat
    14161 ctaacgattg aatagttgcc ttttgacaag cgggtgattt tatcggtttt tttgccgatt
    14221 tttcagaata aaaaatagcc attgttttca gatgaattgt tatgatataa agtgtcgaaa
    14281 tggggagatt atataagaaa agcagttggc ggaaaatgga ggcaatatga tttcatcggt
    14341 ttggaatgaa cgttatttgg cggataaaaa acgggaacaa gatcaccgct cgccttttca
    14401 gcgggatcgg gcaaggttat tgcattcgga ggcttttcgc tgtttgcagg cgaaaacgca
    14461 aattcacgcc gtgggggaag atgattttta tcgaacccgt ttaacccatt cgttggaagt
    14521 ggctcaaatc ggtagcagtt tgcgagaaaa attattagcg gatgtggaag gctttcaagc
    14581 ggtctgttcg gagcaggaat ttgcaaaaaa tatggcaaat ctgaccgctt tattgccgga
    14641 tcatagcttg gtggaatccc tttgttttgc ccacgatctc gggcaccctc cgttcgggca
    14701 cggtggtgaa atggcgttaa attataaaat gtacgccttt ggcggcttcg agggtaatgc
    14761 tcaatccttt cggattatga cccagcttga gccttacacc gaaacagccg gaatgaatct
    14821 gacccgtcgc accttactgg gggtaatgaa atatccggca ctcttgtctc aaacctcgcc
    14881 gattgagccg gcagaaatga gcgaggctcg gcatattaat ttgcaccatt ttccacagag
    14941 caaagggatt tttgatgaca acaaccactt ttttgagtgg gtattagccc cgttaagtga
    15001 aaaagaccga cgccaatttt gcacggttca gcccaatcaa gatccgaaac agccaaacaa
    15061 aacgcaatat aaatctctcg attgcagcat aatggaacta gcggacgata ttgcctatgg
    15121 cgtacacgat ttggaagatg cgattgtagg tggaatggtc acaccgcaat cgtggcaaaa
    15181 tgcggaaaaa ttgctggcgg agtgccaatc cgattgggtg aaacaacgcc tgccggagat
    15241 tcgggaaaag ctcttttctc agcaccgcta cgagcgaaaa gatgtgatcg gggcattagt
    15301 gaatcatttt attaccaatg tgcgttggaa agctctgcct gaatttgacg agccgttgct
    15361 acgttataat gcctatttac cggaaagtgt tgcctgtgtg ctgaaaatct tgaaagattt
    15421 tgtgtatcaa tatgtgattt gtgatgccaa aacccagcgg gtggaacgca aggggcaacg
    15481 cattctaatg gaattgttcg atattttaag cagtgatccg atgcgattac tccctaataa
    15541 tgtgcgggaa cgttggaaaa atgcggaaga atctaagcgt gcaagggtaa tttgcgacta
    15601 tttagccggt atgtctgatg ggcaggcgtt taaacttttt gaaagtttat aatgaacttt
    15661 gttaattttg caaggtctca gtaataggtt tcatttattt aataggagat ttcaatgaaa
    15721 actgtttcta aaggtctttt agtattagca actttggctt ctgcaacagc atttgcggtt
    15781 tcttccaagt gaaatttatc agcctcgtgg cgaattagtt aaagcagatc gtcaaggtaa
    15841 tggtgagttt gaagtagaat atcgtgtcag tggtaatgat gttcgtggct tagcgaaaag
    15901 tgcgatcgcc cacgctaaac gtaaaggttt ccatttagta gaatctgata ttcatagaga
    15961 tgatgctgat ttaaaattca aacgtggtga ccaagagctg gatattgaaa ttgaagtaaa
    16021 aggtagaaat cggattgaat ataaggcaga tctagattta gataaaaact aaatttaccc
    16081 cttaaaacga ccgcttgtgt gatttaaccg cacaagcggt ctttttattg tgcttttttg
    16141 caaaaatact caagccgacc ggtcccggaa tttggtaaaa tagccgttta aatgacaata
    16201 atgatgcttt tacttattac ttagaggaaa aaatgttttt agtcggtcag cgttggctaa
    16261 gtgaatcgga aaataattta ggtttgggaa tcgtgacttc ggtggatcat cgcactgtca
    16321 ctatcaattt cccggcagcg gaagaagagc gggtctatgc aattgcagtt gcacctttaa
    16381 ctcgagtgca gtttcaagcc ggagatagaa ttaacagtat agatggctgg cagttagagg
    16441 tttcagaggt tgtggaaaat caaggtgccg taatctattt gggcaaaagg gtggataacg
    16501 gtgaagaagc ggtactgcct gaaatgttgc tcgatcataa aatcagtttc agcaaaccgc
    16561 aagaccgcct tttttcggct cagattgatc gctcagatcg ctttgcatta cgctttaacg
    16621 ctttgcagca ccagcaagcg caatatcagt cgccactgcg tggtttgcga ggcattcgag
    16681 ccagcctgat tccacatcaa ttacatattg caaaagaagt cgggcaacgc cttgctcctc
    16741 gtgtgctatt agccgatgaa gtgggactgg gtaaaactat tgaagctggt atgattttgc
    16801 aacaacagct attttcgggc agagcagaac gagtattggt attagtgccg gaaagtttgc
    16861 aacaccaatg gttggtggaa atgcttcgcc gttttaatct gaaattctcg ttattcgatg
    16921 aagaacgttg tagtgatttt gataaacaag atgaaaatgg cgatgatgta agtgaaaatc
    16981 catttgagag cgaagcgtta gtcattactt cactaaactg gctggaaacc tcaccgcaac
    17041 gagccaatca agtgcttgct gccgactggg atctattaat tgttgatgaa gctcaccatt
    17101 tagaatggtc ggaaaccgaa tcttctatcg gctatcagtt ggtagagcgt ttatctcaac
    17161 aaattcctgc cgtcttgctt ttaacggcaa caccggagca attagggcaa gagagccatt
    17221 ttgcccgctt ggcgttgtta gattcggatc gtttttatga ttatgccgag tttgtacagg
    17281 agcaagcaca gtataaaccg gtggcagatg cggtgagtag cttattaagc gataaaccgc
    17341 taagtaatga cgagcagaat gcgattgcgg agctgttaag cgagcaagat accgagccga
    17401 tgttccgtgc aattaatagc aaaaaatcgg acgaaaatga ccgcttgcaa gcccgtcaag
    17461 agctgattag tgagctaatt gaccgccatg gaaccagccg ggtgttgttt agaaataccc
    17521 gtcagggagt gaaaggcttc ccacatcggg aataccacca aatcagccta gaaatgccga
    17581 aacaatacca aaatgcgtta aaagtgatgg gaatgatggg gagtgtgagc caaagtgatt
    17641 tgctctatcc tgaatatttg ttccaacgaa tgaacccgaa tgcgaaatgg gctgaattcg
    17701 accctcggat tgattggctc atcactttct taaaaaatca tcgtgatgaa aaagtgctgg
    17761 tgatctgcaa acaggcggac actgcgattg ctttggagca agttttgcgt gagcgtgagg
    17821 cgattcgttc tgccgttttc cacgagaaaa tgtcgattgt tgaacgtgac aaagctgccg
    17881 cctattttgc tcaacaagaa gaaggcgctc aggtgctgat tagttcaagc atcggttcag
    17941 aagggcgaaa tttccaattt gctaaagatt tagtgctgtt taatttgccg gataacccgg
    18001 atttattgga gcaaagtatt gggcgattag atcgtatcgg acagaaaaat gacattcaga
    18061 tttatgtgcc ttgctttaat ttatcggctc aacagatgtt ggcagagtgg tatcacaagg
    18121 gcttgaatgc gtttgaagaa acctgcccga tgggaacagc gatcttccgt gaattaggtg
    18181 aagaattgca acaattcctg caaaaacctg aactttcagc ccaatttgat gattttgttg
    18241 cgaaaaccta taaacgccaa cagtatttga aagccgaact ggagaaagga caggacagat
    18301 tattagaact gaattctaat ggcggagaag cagctcaaca gctcgctaat gatattgcga
    18361 aagaagataa taatccgcag ttggttaatt ttgccttgag tttgtttgat gtgattggcg
    18421 ttgaacagga agatttaggc gagcagagca ttgtgattag cccaaccgga catatgcttg
    18481 tgcctgattt cccggggcta tcggaagatg gcacgaccat tacctttaat cgtgaactgg
    18541 cgttaatgcg ggaagaggtg gaatttctca cttgggatca cccgatgatc cgcaatggta
    18601 ttgatttaat cacctccggc gatattggca aatcagcaat ctcattgctg gtgaataata
    18661 aattgcctgc cggtacatta ttacttgagg cgctttatgt ggtggaagca caagcaccga
    18721 aagggctaaa tttaacccgt ttcctaccgc caaccccgat tcgggtttta gtggataaca
    18781 aaggcaacaa tattgctccg caggtctctt ttgccgggct tgaacgccaa ttaaaaccgg
    18841 tgaataaaca gatggcaaat aaaattgcca aaatggctcg agcggatatt gaaaaattaa
    18901 ttaaagcgag tgaaaaagcg gtagaacctc aggtccaaca ggtgattgaa actgccaaat
    18961 ttgaggcaaa tagcaaatta aaagcagaat tacaccgctt ggaatcactc aaaatggtga
    19021 ataaaaatat tcgagccgat gaagttgaag ccttagaaca acagcttaac gcttcccttg
    19081 cccaacttga aacggcaaat tatcgcttag atagcttgcg agtgattgta agtaataaag
    19141 cgtaattttt tgcaagtacg aaaagtgatt ttttcgatct gtgtcgaaaa aatcactttt
    19201 ctattttgtg tcatccttgc agattattta tgctattcct catattgtaa aaggtataaa
    19261 aaaggaatat tatgtatata gatttgcaat atgctgtgcc gttattgttt gaatacgacc
    19321 cgaacaaaag ccaaattaat ttagccaaat atggtattga ttttgaacag gcaaagttgc
    19381 tttgggaaga tgaacgaaaa gttgtactgg aggcttgcat tgaacccgaa ttacgctatt
    19441 tcctgattgg caagatttat gataaatatt ggacagcgtt ttttacacca agaaatgatg
    19501 ttattcgttt aatttcagtt agacgttccc gtaaaaaaga gatctcatac tatgagaaat
    19561 accattagtg ccgaagaatt agaacgtatc ttcgatgaag gcaaagaaga tattttacaa
    19621 tactgcgatc tttctaccgc tcatcgcccg gggcaaatga aaagggtgaa tgttgatttc
    19681 ccaaattgga tggtagaagc gttagataag caagccagac atttaggtgt gcctcgccaa
    19741 gccttgatta aaatgtggat tgcggaaaaa ttaccggttc aatcacctag gtaagcgagt
    19801 ggcaaatggc attaattgaa tataaccctc cattagaacc atttttagat gaggtttatc
    19861 gggataacca tattttagta attaataagc ctagtggttt attatcggtg ccgggcaatc
    19921 gtcccgaata ttacgacagt gcaatgactc gagtacagca aaaatatggc tttaccgaac
    19981 cggctcatcg tttggatatg gcaacaagcg gtattctttt gttcgcccta agcaaagcgg
    20041 cggaaaaaga actaaaacgc caatttcggg aacgtgaacc gaaaaaacac tatattgcgt
    20101 tactttgggg caaattaggt gaaaaagttg gggataaggg cgaaatgaat tttccgctga
    20161 tttgcgattg ggaaaatcgc ccccgccaga aaatttgcta tgaacgaggc aaaaaagcca
    20221 ttacccatta tgaagtgtta gctcattatc cgaataatat agcgagagta aaactcacgc
    20281 ctatcacggg gcgttcgcat cagctacgag tacatagcct tgcgttaggt cacccgatta
    20341 ttggcgataa gttttatgct aatccgctgg caaaaagttt atcgccccgt ttatgtttgc
    20401 acgcggagtc actaacaatt actcacccga ttacaaggga gttgatgacg tttactgctg
    20461 agccgacttt ttagcctcgt tcagtaggtg tttgtaatat gcccaatcaa aatattgccc
    20521 cgggtcgatt ttccgttccg gggcaatatg gcaatgcccg gtgatgcgtt catcggtaat
    20581 ttgtggatag ctttgctgaa tggctttggt caaatttgcc aaagattggt attgagcttg
    20641 agtaaacggc tgattattac tgccttccag ttcaatgcca atcgaatatt cattgcattt
    20701 ctcccgccct tcaaaacaag agacgccggc gtgccacgcc atatcgttaa aattcacata
    20761 ttgcgtgatt tccccctccc tattaatcaa acaatgggcg gaaacccgta gatctttgat
    20821 ttcttcaaaa taagggtgag cggttggttc gagtttacct tggaaaaaca gatcgataaa
    20881 atttccacca aattcttccg gcggtaagct gatgtagtga ataaccaaca aggaaatatc
    20941 ctcttgatca ggacgttggc taaaatgcgg agacggcact tgtttttgac cggttaaaca
    21001 gccctgttcg atttttaaat ttggcatagt ctaaataggg tgaatacgtt aaaatatggc
    21061 tcattttact agaaaaagag aagaatatgt taagttatcg ccacagtttc cacgcaggca
    21121 accacgctga tgtgctaaaa cacattgtcc agcttttgat cttgcgagca ttaaaagaaa
    21181 aagaaaaagg ttttttatat cttgatactc actccggtgt ggggcgttat agtctgttaa
    21241 gttttgaatc ggaaaaaaca ggcgaatatt tagagggcgt tgcccgttta tgggagcgtg
    21301 atgatttacc ggaagaagtg gcattctata ttgatgagct gaaaaaatta aaccgtggcg
    21361 gcaagttaaa gtattacgca ggctccccat tattggcggt acagcaactg cgtgagcaag
    21421 accgagccat tcttaccgaa cttcacccca atgattttcc gctgttacga caagaatttg
    21481 ccaaagcgaa aaatgtgcaa accaagcgag aaaatggctt tcaacaatta aaatccgccc
    21541 tgccgcctaa agagcgtcga ggttttgtgt tgattgatcc accttatgaa ttaaaagaag
    21601 attatgaatt ggtggttaaa gcgatagaag agggctacaa acggtttgcc actggtgtgt
    21661 atgccatttg gtatccggtg gtgcttcgcc aacataccaa acgtatagtg cgaggtttaa
    21721 tggataccgg cattcgcaaa attctacaaa ttgagcttgc cgttcgtcct gatagcgacc
    21781 aacgtggaat gacggcaagt ggtatgattg tgatcaaccc accgtggcag cttgaaaccc
    21841 agatgaaaaa aatcctgcca tatttaaccg aggtgcttgt gccggaagga acaggttctt
    21901 ggaaggtgga gtggattacg cctgagtaaa ttaaatacgg atagcacaag cgtggacgct
    21961 tgcgccatca taaattctga tttgcaatat tttttagaaa aataaccgct tgtcttatca
    22021 agaagtgggg gaaatataaa aggaaaaaac gatgcaacaa tacaaacacg actttattga
    22081 atttgcctta agccgcaatg tgctgaaatt tggcgaattt accttaaaat caggcagaat
    22141 cagcccctat tttttcaatg ctgggctttt taacaccggg cgcgatttag ccaaattagg
    22201 cgaatattac gccaaagcca ttcaagccag cggcattgag tatgatgtgt tattcgggcc
    22261 ggcttataaa ggcattccga ttgcaaccac ggttgccgtg gcactagcca acctgttcga
    22321 tgtggataaa ccttgttgct ttaaccgcaa agaggcaaaa gatcacggtg aaggcggtaa
    22381 tttaatcggt tctcccttaa cgggcaagat tttgctggtg gacgatgtga ttaccgcagg
    22441 cacggcaatt cgtgagtcga tggaaattat caatgccaat aatgcccagc ttgccggtgt
    22501 gctgattgcg ttaaaccgta aagagaaagg caagggcgag ttgtctgcga ttcaagaagt
    22561 cgaacgtgat tacggttgcc aagttttctc gattgtggat tttgatgatt tggtagcatt
    22621 tattgaaacg tcgccacaat acgctcctta tttgccgaaa atgcgtgaat atcgtgaaaa
    22681 atacggtgtt taacttttgc aaaaaaacag caaaaactca ccgcttgtca ctctctaatc
    22741 tcaccattat gaatcacttt catcttttgg tgagattttt tcaattaaaa agccgataaa
    22801 ttgattgtga gctttagcta attggcttag gataaactcc tgtaatacag gagttggaga
    22861 actaaaatgg cacatcaaaa attacctttt cacgcagaca ccgttggctc atatttgcgt
    22921 tccgatgctt ggaaaaaagc tcacgcagac tataaagcag gcaacatttc cctcgaacaa
    22981 cgtgatgaaa ttgtagaagc ggaagtgaaa aaattagtac aagcatcaat tagatgcggg
    23041 cattcaagtt gttacagacg gcgaatatca ccgctcttgg tggcatattg atttcttgga
    23101 aaatctgaac ggcattgaag gctatgtgcc ggaaaaagcc tatgcgttta aaggggttga
    23161 ggttcgccct tataatacac gttgttgcgg taaagtgtct tggaatccga atcacccttt
    23221 tattgagcat ttcaaaaaat tcaatgcgat tgtaggtggt cgtgcggtgg cgaaatttac
    23281 cattccaagt ccaaaccaat taatgtaccc gattcagtgg gatcacggca tttacccaaa
    23341 ccgtgaggaa tttgccaaag atgtgcaaca ggcttataaa gaggcgatta aagcgttcta
    23401 tgatgccggc tgtcgttatt tgcaaattga tgatgtctat tgggggtcgt tgtgtaataa
    23461 cgttggtcaa ccgacatttg aagccgataa agctcaagcg gtggcgaata ttcaagccat
    23521 tcttgccgat aagccggaag atttaaccat taccacccac gtttgccgag gcaactataa
    23581 atcttcttat ttattgacag gggcttatga tccgattgcc gacggcttat ttgctcaaac
    23641 caactatgat gggtatttct tagaatatga tgacgagcgt tcaggcggtt ttgagccgtt
    23701 aaaacatttt gccaacaaca aagggcgtgt ggtattgggc ttaattagtt caaaattccc
    23761 tgaattggaa gataaggaag cggttaaagc ccgtattcaa gaagcggcaa aatatgtgcc
    23821 gttagagcag ctttgcctaa gcccgcaatg tggttttgcc tcaaccgaag agggcaatat
    23881 tatgtcggaa gcacagcaat gggcgaaagt tcgtcttgtg gaggaaatcg cccaagaagt
    23941 ttggggcgaa gattaattag agtgaaaaat gcccgtaaca agcgggcatt ttttgtgaat
    24001 tttttgcaaa ttcaagccat ttagcgaatg cggttaaaac cttgttctaa atcggcaatc
    24061 aaatcttcca ccgcttccaa gccaacgtga atgcgaatta atgttcccgt cagtttgcgt
    24121 tcaatcgccg ggcggatttt ggcaatttct tccggctgat tggcaagaat cagcgattca
    24181 aacccacccc aagaatacgc cattgaaaac agttcaaaat gctctaggaa gttggctaat
    24241 ttgtcattcg agagcttttc gcaaagttca aatgaaaata gaccgcttga acctaaaaaa
    24301 tcccgcttaa aaaattcgtg ccccgggcag ctttccaacg ccgggtgata aactgctttc
    24361 acttcagggc gttgagccag ccagttagct accgcaatgc tactttgttc gtgctgtttt
    24421 aagcgaatgc ctaaggtgcg tagcccccga gcggtgatgt aagcggaatc agcatccacc
    24481 atttgcccca ttaaataaga gccttcacgc agtttatccc aacaacgggc gttagaaacc
    24541 gcagtaccga tcatcacatc agaatgcccg actaaatatt tagtgccggc ttgaatggaa
    24601 atatcaatgc catattctaa agcagggaac agcacaccgg cagaccaagt gttgtcgatc
    24661 ataatcacga tttctgggtt gatttcacga gcaacacgca cgagagtcgg aatatcaggc
    24721 acttccattg ttagcgagct cggcgattct aaaaaaagca ctttggtgtt cggttgcaca
    24781 agggctttca agccttcacc aatcatcggg tcataataag tagttgaaac acccatacgt
    24841 tttaacagcg cattacagaa ttcttgtgtc ggttcgtaag ctgcgcctgt cattaaaacg
    24901 tggttgcctt gttccacaaa agctaaaatc gcattggtga ctgccgccgc accgcaaggg
    24961 taaaggtagc aacctgctcc accttctaat tcacacatcg cttcttgtaa tgaaaaatga
    25021 gtgagagtgc cacgtctgcc ataaaataat gctcctttgt agcggttttg tgtagcgtgt
    25081 tttttctctt ctaaggtttc aaacactaaa gatgaagcac gctgaatcac cgggttaact
    25141 gctccttgtg tgttacgttt tttacgccct gtatgaacaa gtttggtgga aagattttta
    25201 tagttagaca taacttttcc taaagtaaaa tgcatagggg caaatcaaca tttgcccctt
    25261 tattaataat taacgaaaca taaagttatt tctttgacgg ctacgtttaa attggctgat
    25321 tagcacaatg accgcaacaa ttaagtaaac cgcaaaagta aagattagcc attgtgccat
    25381 tgaaaagccg aacatttccc attggctttg gctacaatta accggaccgg gggcaaacac
    25441 ttgtggtagc cattggtcaa gcggcaaggt ttgcgggaaa ttaactttaa attcgcattg
    25501 attccatggt gatggattga cctgataatc gtggtgtttg attgaaagta ctagcccttt
    25561 aaatgcgcta ccaagccaaa ttagaattgc caagtagcga ataatcgcat atctattacc
    25621 aagaaatcct aaaataccgg caaataaaat accaaataaa gcaactcgct cataaataca
    25681 cattacacaa ggtactaatc ccatcccatg ttggaaataa agcgctgtgc cttcaagtat
    25741 aagacagctt aacattagta aaaaccatgc actgcgagat tgtgatagat ttttaaaaaa
    25801 atagagcata gttattcctc attttgtgaa cttaaggcat tctaccttat ttggtatgag
    25861 ttatctataa actaacgtgt aataaaccat ccccaattta tcattacttc agtcatagaa
    25921 ggtagtaaga attctaaccc taagaaaccg acaatagata gaacaatggt ataaggtaat
    25981 gccatataga ccattctgcc gtaagacagt ttaattaacg gcgcaaagga ggacgttaat
    26041 aagaataaga aagcagcttg tccgtttggg gtagcaacag aaggaagatt tgtgccagta
    26101 ttaattgcaa ctgcaatcag gtcgaactgc tcacgatcta taatgccggc atctagtgcg
    26161 gttttagctt cattaatgta aacagtaccg acgaagacgt tatctgaaat catcgagagc
    26221 aaaccattga aaatatagaa taatgaaagc tgtgaatgtg gttcggcaga taacacaaag
    26281 gcaatgatcg gctcaaataa ttttaaatca ataatcacgg caacaaccga gaaaaacaca
    26341 ataagcaatg cagtaaaagg catagattct tggaatgctt tccctaaagt gtgttcatca
    26401 gtaatgccac aaaatgcagt gcaaataatg atgatggtta agccaataat ccctacatcg
    26461 gcaagatgta gcgctaagcc aataattaac caaattccag caatggcttg caccagcatc
    26521 tttaagcgat cttgtttcgt cattttttgt tcttttagtt ggctatagcg agcgagtacg
    26581 ccccaaactt tgctaggaag ccggtctcca taaccaaata atttatattt ttctacaaga
    26641 aagcaggtcg caatcccaca aattaacacg ggtaagctaa ctggtaatgc tctaagtaag
    26701 aattcgataa atccccattc cgcttgggag gcaataatta agttttgtgg ttctcccacc
    26761 atggtcatta cgccacctaa agctgagcca ataccggcgt gcatcaataa acttcttaaa
    26821 aaaccacgaa attgctctaa aatttcttta ttagagatga ttttttcatc attggtaata
    26881 tcagtagaat cttcaaagtt attacctgat gcgactttat gatatacgcc gtagaaacct
    26941 gttccaacgc taatgattac agcaataacc gttaaagcat caagaaaggc ggataaaaaa
    27001 gcagcgctta agcagaaaga aagggaaaga atttttttcg aatgaatggc gacaagcagt
    27061 ttggtgaaaa tataaagcaa taattgcttc ataaagtaaa taccggcaac cataaacatt
    27121 aaaagaagaa taacatcaaa gtttgccata atttcttctt taatgtggtg aggatttgtc
    27181 ataccgatta caactgcttc aatcgctaat aaaccacctg gctgtaatgg ataacatttt
    27241 aatgccatag agagagtaaa aataaattca gcgactagaa accagcctgc aacaaaagga
    27301 ttgataaaga aataaatgag aggattgata attaaaaatg ccaaaatgca caatttatac
    27361 cactcggggc tttttcctaa gaaacttttg aacagagcat caatgctact catataaagg
    27421 acctcaaaat ttgtaaacag atattacaat tgtaatatag tttagattaa aggataatag
    27481 tattactgaa ctttttagtc gcaataattg attagaaata aagaatagtt agattttaga
    27541 taagcatatt ttttgcttgg ggggcattaa tgggtcacag tgatatttta aaagcgcaaa
    27601 gcccggcagc gttagcggaa gaatatattg taaagagtat ttggaataat catttccctg
    27661 ccggctctga tcttcctgcc gagcgagagt tagcagagcg cattggggta acaagaacaa
    27721 ctttacgtga agtactacaa cgtctagcgc gagaaggttg gttaagtatt cagcacggca
    27781 agccaacaag agtgaatgat atttgggaaa cttcggggcc taatattatt ggcactatta
    27841 ttaagctaga taaaagctat cttccagtgg tgattgccaa tgtggtgtca ttacgtactc
    27901 gtatggcgga ggcttatatt ccagaagcga tttaccttgc tcctgaagaa agtgctaaac
    27961 tctttgatgg actagatgaa ttagaaaata cagcagctgc ttatgctgaa tttgattata
    28021 agttattcag acaatttact tttttagcca ataagcctgt gtatgcgttg atcctaaata
    28081 gctttaaatc aatgtatcat caagtcgcaa aaattttctt tgctgatgaa aataatcgtc
    28141 aattaacgtt aagcttttac cgttcattat tatgtacttg tcttgcaaaa gatagtgaaa
    28201 aagcagccgc ttgtatggct aaaaatcgcc aatctagtgg tgaaatttgg caaaaattat
    28261 ccgctaatat tcctgaaagc tttggtcagc tttaacagaa ggtagaattt tgaacgaaat
    28321 tatttttagc ttagaaccgc aagataatgc tcgtctgcaa tcgctttgtg gtgcatttga
    28381 tgagcattta gccttaattg aaaaaagttt taatctcatt attgctcgaa caggcttcaa
    28441 ctttacgatt caatcggaag atgacaatca ctattctgct accttggttc aaaatagtgt
    28501 gaagctgatt aagcaactgt ataatgaaac tgccccgatt aaaggcaaaa ttaaggaatt
    28561 ggacttagaa gatatacatt tggcgattca ggaaagccga atgctactac aaaaccaatg
    28621 gcaagccggc tcgcaaggcg aaatagcgat taaaaccaag cgaggggtga ttaaacctcg
    28681 tagcgaacat caacaagcct atttacgaaa tatattaagc cacgacatca gttttggtat
    28741 cgggcctgct ggcacaggca aaaccttttt agcggtagcg gcagcggtgg aatcgcttga
    28801 acgccaagaa atccgccgta ttttacttac ccgtccggcg gtggaagccg gtgaaaagct
    28861 cggtttttta cccggcgatt tagggcaaaa aattgagcct tacttacgcc cgttatatga
    28921 tgccctgttt gaaatgcttg gctttgaaaa agctcaaaag ttgatggaac gtggtgtgat
    28981 tgaaattgcc ccacttgctt atatgcgagg gcgtacttta aacgatgcgt tcatcatttt
    29041 agatgaaagc caaaacacga ctaccgagca aatgaaaatg ttcttaaccc gtattggctt
    29101 taattccaaa gcggtgatta ccggcgatat tacccaagtg gatttgcccc gtagccaaaa
    29161 atcaggctta aaacacgcaa tggaagtgct aaaagaagtg ccggaactca gctttaattt
    29221 ctttgacagc aaagatattg tccgccaccc tgtggttgcc aaaattgtgc aagcctatga
    29281 tgcgtgggaa gcggttgatg aagagcgtaa agaacagcga aaactggaaa aattagcttt
    29341 agagcaagaa aaaattcttg ctcaagctga aatgttatag cttacaagcg gtcatatttc
    29401 gctaattttt tgcaaaaaat cgctgaaatt tcaccgctta tcataacagt tgttcaacaa
    29461 aaaagcggga agttaattcc cgcttttctt tatcttattg tttcattata agatttcttt
    29521 tgctttagca accacgtttt caacggtaaa gccgaagagt ttgaataatt gatctgctgg
    29581 tgcggattca ccaaagctat tcatacctac aattcgacca tcaaagccga catatttata
    29641 ccagaagtca gctaattgag cttcaatcgc cacacgttta gtgactgaac gtggtaatac
    29701 cgattcacga taggcttcat cttgtttgtc aaacacgttg gtgcttggca ttgaaactac
    29761 acgcactttc acgccttctg catctaacac atcagcggct ttcattgcta aatctacttc
    29821 agaaccggtt gcaattaaga ttaaatccgg gcaactgcct ttttcgcaac attcacgcaa
    29881 gatatagcca ccacgagaga tgttcgctaa ttgctccgca gtacgttgtt gttgagccag
    29941 attttggcgg gtaaagatta gtgctgtcgg gccgtcttta cgttctaccg ccgctttcca
    30001 agcaacagca gattctactt ggtctgccgg acgccaggtt tctaagtttg gaatttggcg
    30061 taatgaagca gtttgttcta ccggttggtg ggtcgggccg tcttcaccta aaccgatgga
    30121 gtcgtgtgta taaacaaata aggcacgttg cttcattaag gctgccatac gcaccgcatt
    30181 gtgtgcatat tcgtagaaca ttaagaaggt tgcaccgtaa ggaatgaaac caccgtgtag
    30241 ggcaatcccg ttcataatcg cagacatacc gaattcacgc acgccgtagt tgatgtagtt
    30301 accatctaca ttgtgttccg cacgaatcgg ttttgagcca gaccataagg ttaagttaga
    30361 gcttgctaag tcagctgagc cgccgaggaa ttcaggtaag attggcgcgt aagcttcgat
    30421 tgcattttgt gatgctttac ggcttgcaat ggttgccgga tttgcttgta atttttcaat
    30481 aaaagctttg ctttcagctt cccagttagc cggtaattcg cctgcgtaac ggcgtttaaa
    30541 ttcagcggct agttcagggt atgccgcttc ataagcagca aatttagcgt tccaatcttt
    30601 ttctgctaat gtaccttttg cttttgcatc ccactctgta taaatttcag ctggaatcac
    30661 aaatggggca tgttcccaac ctaaaaattc acgagcggct tggatttcag catcacccaa
    30721 tggcgcaccg tggcaatcgt gagagtttga tttatttggt gaaccatagc cgataatggt
    30781 tttacaaata attaaagttg gacgctcggt ttcggcttgt gcattttcaa tcgcaaattt
    30841 gatttgttct gggttatggc catcgacatt acggataact tgccagccat aagactcaaa
    30901 gcgttgtgcg gtatcatcag tgaaccaacc atcaacgtgg ccgtcgatag agatattgtt
    30961 atcatcgtag aaagcaatca gtttgcctaa gcctaatgta ccggcgagag agcaagcttc
    31021 gtgtgagata ccttccatta aacagccatc gcctaagaaa gcgtaagtgt agtgatcgac
    31081 aattttatga ccttcacggt taaattgtgc tgcaagggtt ttttccgcaa tcgctatacc
    31141 gaccgcattg gtaatacctt gacctaacgg gcctgtggtg gtttcaacac ccggtgcata
    31201 accatattca gggtgaccag gtgttttaga atgtaactga cggaactgtt ttaaatcttc
    31261 aatagagaga tcatagccgg ttaaatgaag taagctatag attaacattg aaccgtgtcc
    31321 gttagacaga acaaaacggt cacgatctgc ccatttagga ttagttggat tgtgttttaa
    31381 aaaatcacgc cataaaactt ctgcaatatc agccataccc attggtgcgc ctgggtggcc
    31441 tgagttagct ttttgtacag catccatact taaaaaacga attgcattcg ctagcacttt
    31501 acgttctgtc atttgtgtta ctcctaatcg agaatgttat ataaaatgga aaggatatcg
    31561 aattggattc tacactattt tgctagaaaa actcaaaaat ggaaatgaga aatgtgatct
    31621 gtatcacaaa aaatgattaa attgtgattt gttttgat
//
LOCUS       DANLXS010000008        30481 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.008, whole genome shotgun sequence.
ACCESSION   DANLXS010000008 DANLXS010000000
VERSION     DANLXS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 30481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30481
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.008"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            5..505
                     /locus_tag="PXD71_000297"
     CDS             5..505
                     /locus_tag="PXD71_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL6290463.1"
                     /translation="MSNIGLDKEIAKKLADELNGLLATYQVFYTNVRGYHWNVRGVNF
                     FELHAKFEEIYDDLVTKVDEIAERILTLGHTPQNAFSQYLTKSQIQEHIGVSAAQECL
                     RGTLNGFKTLLGQQREILALANDANDEGTASQMSDYIKEQEKLVWMFTAACAECSDYA
                     QGCSCN"
     gene            564..1277
                     /locus_tag="PXD71_000298"
     CDS             564..1277
                     /locus_tag="PXD71_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDL6290464.1"
                     /translation="MAMSQGFQFKQFFIRHDRCAMKVNTDGILLGAIAHIKDCQNILD
                     LGIGSGLVALMLAQRTDDCCHITALELEPNAYQQAVENAQNSAWANRISVLQGDVMQQ
                     AFEQKFDLIVSNPPYFADSLAARTTERDLARSISHYSHLNWLAKAKPWLAENGKITLI
                     LPIAAAEKLVDQSEQIGLYCVEQWLIFTKSGKLAKRSIVSFSLNKQKREHKELIIYDL
                     DNCYTTEFKRLTQDFYLNF"
     gene            complement(1388..2392)
                     /locus_tag="PXD71_000299"
     CDS             complement(1388..2392)
                     /locus_tag="PXD71_000299"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL6290465.1"
                     /translation="MAFNLKNRHLLSLVNHTEREIRFLLDLARDLKRAKYAGTEQQKL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGAHVTYIDPNSSQIGHKESMKDTARVLGRMY
                     DAIQYRGFKQSVVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIENCEKPLSQISYV
                     YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEDAFVKMCEKFAAESGARMTVTED
                     IETAIKGVDFVHTDVWVSMGEPLESWTERIDLLMPYRVTPELMQATDNPKVKFMHCLP
                     AFHNSETKVGKEIAEKYPALANGIEVTEEVFESPMNVAFEQAENRMHTIKAVMVASLA
                     "
     gene            complement(2480..3319)
                     /locus_tag="PXD71_000300"
     CDS             complement(2480..3319)
                     /locus_tag="PXD71_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL6290466.1"
                     /translation="MQSYPYLLVNVFATSHFGGNPLAVFYEADGLTDLQMQLIARQFN
                     LSEVVFIQSSTDSQAVKKLKIFTPDYEMPFAGHPTVGAAFVLHSAFDFPTTYRLQTNA
                     GLVAIQHQHNVTTFALTNGVNLENTPLSRAECASILGLNVADIASEPCYVNTGVEQLL
                     VELASQQAVEKCKINANLAEKAFLKDSVYVWCREGNEAKVRLFFQSQGSIVEDPGTGS
                     AAANLGGWHIAQGKIPIQLQISQGDEIQRPNRLLLKVDQNGKIFVGGEVILVGKGEFY
                     LPE"
     gene            3509..4843
                     /locus_tag="PXD71_000301"
     CDS             3509..4843
                     /locus_tag="PXD71_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL6290467.1"
                     /translation="MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLS
                     APPISVMLVILAVVTCASILEAAGGLKFMLQVAERILRSNPKRITFLGPLVTYLMTLM
                     LGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEIVK
                     LPGFEGVTLLNVVGVTITATFCGVIAMSLYSLRRGKELEDDPEYQRRMQDPELRKQIE
                     QTSATSLDEKLPASAKNSVYLFLAAIATIVIIAMIPAIKPTVAATGKVAGMDQIIQIV
                     MLTFGGLILILTKTDPKKVPNGIVFKSGMVATIAIFGIAWMSDTYFTHAMPAFKEGVT
                     EMVRAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAMGLPAPLLVGLMPATYAYFFI
                     PNYPSDIATVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACIVGIAVANVLI"
     gene            complement(4923..5333)
                     /locus_tag="PXD71_000302"
     CDS             complement(4923..5333)
                     /locus_tag="PXD71_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL6290468.1"
                     /translation="MNSTLTNLQPLAFLITRVVAGYMFLLHGTAKFFEFPMSMTNGNG
                     SVPLASIYGVGGILEIVGGIFFILGLFTRQTAFILSGMMAYAFFIAHGAQGNVLLPLL
                     NGGELAGVYSVLFLTYIFIGGGKYSLDAKLFNKA"
     gene            complement(5456..6133)
                     /locus_tag="PXD71_000303"
     CDS             complement(5456..6133)
                     /locus_tag="PXD71_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL6290469.1"
                     /translation="MNLFGFRCFHCNHPLAIGVQGICSHCQKLLKPTPYCRHCGMSAV
                     EFRNACGYCLKNEPKWHKLVRVNEYRAPLSHWVHQFKFQQQYWWDQALARQLLLAIKQ
                     AQREQVLSLPEVIMPVPLFWHRHWKRGFNQSELLCDWLSKWLQIPLDTQSLQRVRATV
                     SQRELTAAERRKNLRGAFFYQPLKAYKRVAIVDDVLTTGSTLNVICAELLKAGVVEIQ
                     VWVLARA"
     gene            6251..7063
                     /locus_tag="PXD71_000304"
     CDS             6251..7063
                     /locus_tag="PXD71_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL6290470.1"
                     /translation="MKSLQKPIKIVFFDIDDTLYVKHKAYIPKSITEQVLPRLKAKGI
                     IPAIATGRNHGCFPEALKPYLNPEIGFELFVTINGQYNFYKDQIISDYSLSREQIERI
                     IEKANALGILYAFITPMTIAVSETNPIVHEAVLPITPNYLIDPHYYEKEKIVQMLLFY
                     TEEQKQAVENSGIFANDLKEARWHPNAVDILRKENSKARGIQDVLATLNIEIESAMAF
                     GDGINDLEMLPTVGVGVAMGNGVPELKAVADFITKPLEEDGILYALETLEVI"
     gene            complement(7111..7380)
                     /locus_tag="PXD71_000305"
     CDS             complement(7111..7380)
                     /locus_tag="PXD71_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL6290471.1"
                     /translation="MGLLYLLLLNTLIGYGSYGEALNHWDTSKVSIVTTMLPIFTMIF
                     SNLSYYFYPHIFAKPDMNWISYLGALVVVSGAILAIAGDKLFRRN"
     gene            complement(7353..8030)
                     /locus_tag="PXD71_000306"
     CDS             complement(7353..8030)
                     /locus_tag="PXD71_000306"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6290472.1"
                     /translation="MKQRPLLGFSLALTAAMTWGTLPIAAQQVLKVIDAQTLVWVRFV
                     VASVGLLLILGFAKRLPKLTACSKKELGLILLGVCGLSANFFLFAEALHYVSPTINQV
                     LWQLAPFSMILLGVLLFKEKFGLFQKIGFVLLIAGLVAFFNDKFAEILQLNTYALGVL
                     LSAGASLIWVLYGVAQKLLLAKFNSQQVLFIIYIGCSLLMLPFASPAQLGNLEDGFLW
                     GCFIYCC"
     gene            complement(8132..8389)
                     /gene="rpmA"
                     /locus_tag="PXD71_000307"
     CDS             complement(8132..8389)
                     /gene="rpmA"
                     /locus_tag="PXD71_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL6290473.1"
                     /translation="MATKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGSNVGMGKDHTLFATADGKVKFEVKGEKSRKYVSIIAD"
     gene            complement(8410..8721)
                     /gene="rplU"
                     /locus_tag="PXD71_000308"
     CDS             complement(8410..8721)
                     /gene="rplU"
                     /locus_tag="PXD71_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL6290474.1"
                     /translation="MYAVFQSGGKQHRVSEGQVVRLEKLELATGEKVEFDSVLMVVNG
                     EDVKIGAPVVAGAKVVAEVVAQGRGEKVKIVKFRRRKHSRKQQGHRQWFTEVKITGIQ
                     A"
     gene            8999..9997
                     /gene="ispB"
                     /locus_tag="PXD71_000309"
     CDS             8999..9997
                     /gene="ispB"
                     /locus_tag="PXD71_000309"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDL6290475.1"
                     /translation="MAVQLFSLEQIQALANEDMQAVNAEILAQLNSEVLLINQLGHYI
                     IAGGGKRIRPLIAVLAARALGYRGKKHITAAAFIEFVHTATLLHDDVVDESDMRRGRP
                     TANAHFGNAASVLVGDFIYTRSFQMMTDLDSLRVLKVMSAATNALAEGEVQQLMNVND
                     PNTTEANYMRVIYNKTARLFEAATQCVAIAADASEEIETALKNYGCYLGTAFQLIDDI
                     LDYSAQSESLGKNVGDDFVEGKPTLPLLHAMHAADKAGKTTQAELIRNAIENGGELEQ
                     IPQVLAIMQEHQSLDYAMQKAREEAQKAVDSLGVLPESEYKQALVSLAYLSVDRSY"
     gene            10100..10846
                     /locus_tag="PXD71_000310"
     CDS             10100..10846
                     /locus_tag="PXD71_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL6290476.1"
                     /translation="MTEQNQRPQEHIESVRARRRAQNQKKGPNAPFVREKLTLPNGHN
                     KLLLHSCCAPCSGEVMEAIHASGIEFTIYFYNPNIHPLKEYLIRKEENIRFAEKWGIP
                     FIDLDDDYENDRKEWFKKAKGMEWEPERGIRCTMCFDMRFEKAAEYAHNNGFPVFTSC
                     LGISRWKDMNQINDCGHRAAAKFDDVVYWDYNWRKGGGSQRMIEISKRERFYQQEYCG
                     CVYSLRDSNKWRLETGRQRIEIGKLYYSPD"
     gene            11263..12198
                     /gene="fabD"
                     /locus_tag="PXD71_000311"
     CDS             11263..12198
                     /gene="fabD"
                     /locus_tag="PXD71_000311"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL6290477.1"
                     /translation="MSKFAMVFPGQGSQAVGMLADLAPQYPIVEETFKQASDVLGYDL
                     WDLVQNGTAEELGQTHRTQPALLASSVALFRIWQQKFPEQKPAVMAGHSLGEYSALVC
                     AGVLDFQDAIKLVELRGRAMQEAVPAGSGAMFAIIGLDNESIIKACEQAASETGEIVS
                     AVNFNSPGQVVIAGTKAAAEKAGELCKAAGAKRALPLAVSVPSHCALMKPAAEKLAEA
                     LQNIELNQPLVPVINNVDVAVETDADTIRNALVRQLYSPVRWTETVEKMAQDGITTLY
                     EIGPNKVLTGLAGRIVKELSAKAVNDVASFEAVEA"
     gene            12215..12679
                     /locus_tag="PXD71_000312"
     CDS             12215..12679
                     /locus_tag="PXD71_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290478.1"
                     /translation="MSKETLFMLISALIALVILPLAYTVGIAKETLGGNMGMLSIAAG
                     CSFLAYFLSLNPKIRHAFIGGSAITAAAIQAWVLYGVLLSENDPKSAVVLFQIYLFSS
                     LGPLFFSAIWAFTRPRWIYPKQGSAVKYALFGGLTTVINYLITLFIFYILTL"
     gene            12700..13422
                     /gene="fabG"
                     /locus_tag="PXD71_000313"
     CDS             12700..13422
                     /gene="fabG"
                     /locus_tag="PXD71_000313"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL6290479.1"
                     /translation="MSKIALVTGATRGIGKAIAEELVAKGYIVVGTATSEKGAESISA
                     YLGENGKGLVLNVADAESIESVLKQIKETFGDVDVLVNNAGITRDGLLMRMKDEDWFD
                     IIQTNLTSVYRLSKAVLRPMMKKGGRIITIGSVVGSMGNPGQTNYCAAKAGLVGFSKS
                     LAKEVASRGITVNVVAPGFIATDMTDELTEDQKNAILSQIPAGELGSPQDIAKAVAFL
                     ASDDARYINGETIHVNGGLYMS"
     gene            13541..14839
                     /gene="brnQ"
                     /locus_tag="PXD71_000314"
     CDS             13541..14839
                     /gene="brnQ"
                     /locus_tag="PXD71_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL6290480.1"
                     /translation="MTRNTLVVGFLLFAIFFGAGNLIFPPKLGYESSTEFFSAISGFV
                     LTGVGLPLLGIIVSAFYNGGYKSALDKIHPWFSLAFLTSIYLAIGPFFAGPRTAATAY
                     EMAIMPFLGDSSQVSMLIFTLIYFAFTLWLALNPNKMVDRIGAILTPILLITIIGLVI
                     RAGFILDTDPQITTPNTNEQSYFFKGMVDGYLTMDALASLAYSVIVLNAIRGKGVSQA
                     ALQRQTVSAGLVAAVALAVIYISLGWIGNNMPISANTLSMLSERKLDLGTYILNTVTT
                     QAFGELGRSVLGVIVSLACLTTAVGLAVSVSEYFNEIFPKISYKVYAILFTVISFTVA
                     NQGLKEVISKSIPVLLVLYPIAMTVILMLLINLVIKLPLSAQRLSLLLVTIVAIASVA
                     GVGFTENLPLKSYSMEWLPFAIIGAIIGAIIHPLTGRKHK"
     gene            14885..15709
                     /gene="mutM"
                     /locus_tag="PXD71_000315"
     CDS             14885..15709
                     /gene="mutM"
                     /locus_tag="PXD71_000315"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDL6290481.1"
                     /translation="MPELPEVETSVRGVSPYLVGQTIEQIIVRQPKLRWTVSPELSQM
                     AGAKITAVERRAKYLIIRTDKGDILVHLGMSGSLGILQERQQKEIGKHDHVDLITQNG
                     TILRYNDPRKFGCWLWAEKAEDHELLRKLGPEPLSNDFTADYLFAKSRKKTTACKTFI
                     MTNEVVVGVGNIYACESLFMAGIHPELAAQNLTKKQCERLVKTIKEVLTKAIIQGGTT
                     LKDFIQPDGKPGYFAQVLQVYGRKGETCNDCGKPIETKVIGQRNTYFCPKCQRLPK"
     gene            complement(15792..16226)
                     /gene="dtd"
                     /locus_tag="PXD71_000316"
     CDS             complement(15792..16226)
                     /gene="dtd"
                     /locus_tag="PXD71_000316"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL6290482.1"
                     /translation="MIGLIQRVKFAKVEVEGQVVGEIASGLLVLLGVEKDDDEAKADK
                     LLEKVLNYRVFSDSEGKMNLNVQQAGGSLLVVSQFTLAADTNKGLRPSFSKGASPVDA
                     ERLYAYFHQQAATKIHTQTGQFAADMQVSLQNDGPVTFWLQV"
     gene            complement(16201..17046)
                     /locus_tag="PXD71_000317"
     CDS             complement(16201..17046)
                     /locus_tag="PXD71_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDL6290483.1"
                     /translation="MLNRLWRFIKLFAKRYNENNIAISAGYLTYSTMLAIVPFIVVLF
                     SIFTFFPIFHEANEQLKAFIYDNFAPSASEMVSEYIELFVSNAKQMGLVSFIGLFVVT
                     IMLIKSIDEALNTIWHDNRQRPMWFSFLLYFLILLFGPLLAGASIAISTYVMSLSIFN
                     GNGALSLGQHLLELMPFLLIWLLFTLVYKFIPIRKVKFQHAAIGALMAAIFFTLGKKA
                     FIWYITAFPSYQAIYGALAVLPIMLLWIQLSWQVVLLGGQFAAVLDEMETDKGSINDR
                     LNSAG"
     gene            complement(17048..18286)
                     /gene="proA"
                     /locus_tag="PXD71_000318"
     CDS             complement(17048..18286)
                     /gene="proA"
                     /locus_tag="PXD71_000318"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL6290484.1"
                     /translation="MQMIELAQQAKQASFELAQFGNLQKNQALLTIADSLEQRAEEIL
                     YANAKDIEFAQAQGISLAIIDRLLLNEDRLKDIANDVRNVAKLADPVGQVMDGGVLDS
                     GLKIERQRVPLGVILTIYEARPNVTIDVASLCLKTGNAVILRGGKETKFTNAVLVDVV
                     QDALEKAGLPKLAVQAVTDPDRALLLELLKLDRYIDMVIPRGGAGLHQFCKENSTIPV
                     IVGGIGVCHLFVEKTADQEKSLEVIANAKTQRPSTCNTLETLLVEKAIAVEFLPKLVA
                     KMQQLGVTIHSNDLQKIEGIEPLDQAKLSQEWLSLDLNVVVVNDLQQAVEHIRHYGSQ
                     HSEGILTSDYQLARQFVQQVDAAAVYINASTRFTDGGEFGLGAEVAVSTQKLHARGPM
                     GLEALTTYKWVCEGDYLVRG"
     gene            18570..20453
                     /gene="mutL"
                     /locus_tag="PXD71_000319"
     CDS             18570..20453
                     /gene="mutL"
                     /locus_tag="PXD71_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL6290485.1"
                     /translation="MINTTPKQLIHILPPQLANQIAAGEVVERPASVVKELVENSLDA
                     GANTIQIDIEKGGSQLIRIRDNGCGISKQDLPLALARHATSKISSLEDLEAILSLGFR
                     GEALASISSVSRLTLTSRTAEQTEAWQAYAQGREMAVEIQPASHPIGTTIEVANLFFN
                     TPARRKFLRTDKTEFAHIDEVVRRIALAKPNISFSLSHNGKIVRQYRKVQDNSVEQQQ
                     KRVAAICGDEFIQHSTYIDRQHGDLHLHGWVGSPALARLQNDLCYSYVNGRMMRDKTI
                     NHAIRQAYGDSIASGYYPAFVIFLDLDPTMVDVNVHPAKHEVRFHQGRLVHDFILQGV
                     LNSLQQTQPTLELRNEVNEPLPSYVQDTNRQAAGQNIFVSQTAPQAAEFTQKNASRSS
                     YTPKFERSNSVSSSAQKWYKELVGGDLHQTTPTIKTQSTLVEPKTEPQTEHKTEAVSQ
                     KITQIAPLASHHSQALAIVKNQAVLIKEEENFYLLPLATLAELKLKLQLEKGQSQALL
                     IPLNLSLDQQQTERWQQTKEELTKLGFSISEKQWQAQTRLTVLSVPQAVREQNLQQLL
                     FALFNQTQAVNLAEFFAKKCEKPTACTLSDVIALLSEIELYANGKKELEGIRKEVDFS
                     KFL"
     gene            complement(20472..20741)
                     /locus_tag="PXD71_000320"
     CDS             complement(20472..20741)
                     /locus_tag="PXD71_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL6290486.1"
                     /translation="MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYC
                     DHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLRIGSHSHLFG"
     gene            complement(20728..20994)
                     /locus_tag="PXD71_000321"
     CDS             complement(20728..20994)
                     /locus_tag="PXD71_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL6290487.1"
                     /translation="MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATE
                     KRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADLMEQLNARD"
     gene            21434..22387
                     /gene="miaA"
                     /locus_tag="PXD71_000322"
     CDS             21434..22387
                     /gene="miaA"
                     /locus_tag="PXD71_000322"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL6290488.1"
                     /translation="MNKKPLALFLMGPTASGKTDLAIALRQELPVEVISVDSALIYKG
                     MDIGTAKPSKEELALAPHRLIDILDPAESYSAMNFHADALREMADITSQGKIPLLVGG
                     TMLYYKALLEGLSPLPSADPAIRAEIESRAAQHGWATLHQELVKIDPIAGARINPNDS
                     QRINRALEVFYITGKTMTELTAQQGEALPYNILQFAIAPQDRAVLHQRIEQRFHKMIE
                     LGFEEEVKRLFLRKDLHINLPSIRCVGYRQMWEYLQGDISLDEAIFKGICATRQLAKR
                     QITWLRGWQGELTWLDSLAPTSSKAKMVEKIDHYLKNCDNI"
     gene            22456..22728
                     /gene="hfq"
                     /locus_tag="PXD71_000323"
     CDS             22456..22728
                     /gene="hfq"
                     /locus_tag="PXD71_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDL6290489.1"
                     /translation="MAKGQSLQDPYLNALRRERIPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPARALSHNNNNQAQHAQPAAEVTEQAE"
     gene            22748..24133
                     /gene="hflX"
                     /locus_tag="PXD71_000324"
     CDS             22748..24133
                     /gene="hflX"
                     /locus_tag="PXD71_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL6290490.1"
                     /translation="MLNESLPNSEENSAFGFALSEASQTTEAASPTDPENQDKAILVH
                     LYLGQHKDVENLLEFKTLAESAGVEVLATLTTSRQSSHIKYYVGQGKAEGIAEAVERL
                     GATVVLVNHQLSPAQTRNLQSLCGCRVVDRTGLILDIFAQRARSHEGKLQVELAQLRH
                     LSTRLVRRVTNQDQQKGGAVGLRGPGETQLETDRRLIKVRIQQLLNRLEKVSKQRNQN
                     RKTRQKADIPTVSLVGYTNAGKSTLFNAITQAGVYAADQLFATLDPTLRRIQVQDVGT
                     TILADTVGFIRFLPHDLVSAFKSTLQETTEATLLLHVIDGSDERKNENIDAVNQVLDE
                     IGALDIPTLLVFNKIDKLEGVEPHIERNADGTPVAVYLSAQENSGIELLFQAIQERLR
                     SNLVCENLLLPVTAGAIYAQFKAENCIKTEHFNEFGERIISIEINEVQWNKWVKQFPD
                     LAEYIELAKWG"
     gene            complement(24355..30426)
                     /locus_tag="PXD71_000325"
     CDS             complement(24355..30426)
                     /locus_tag="PXD71_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL6290491.1"
                     /translation="MNKIFKVIFNQTTQKMEVVSELARSQGKAASSTDKRGTIKLAAA
                     SILGGATLISGMAAFMLSATPAEAAVIIDRIEVQQKAPKKVVNGGIDIYTNTSLATDS
                     ITNPYNYFNPGSLSYADRTKLQESNNSVNKEKDSRLLYKSNTTSAIVIGHNAVALDNN
                     RKSRGKPKASGIAIGDYSYSGGGLSIAIGSFSRTASIGSVALGTSARAEGFNSFAAMR
                     QSAAIADYSTAIGSTSWANATASFAIGASATALGDRSFAIGSGTPQTVDGSYGHENRR
                     SIYDGQNNTLAFGTDAFSIGTKAKTNGNHSFAIGTNAWSGGFKLENSNISELGQVINP
                     DTAKVANNAFALGTNTRANSNDTLAFSTNASATGDGSIAFGVNSNTTGIGAIAFGNAS
                     KAWKSNAISIGWNANSHAEKVISIGSHSNASKDGAMAIGDTANSSGNNSVAIGYSANV
                     SKANATAVGTRANITHEGGTAIGFEAKSKGNYATAIAYNSTAKGKNSIAAGVNSTVSG
                     IRAAALGYNNTVSGKDTFVLGSNVTVKTKGSVILGSDSTEATATKEDTATVGTLKYSS
                     FAGDENVVDGDYVSVGNATNARQIKFVAPGKIASNSTDAINGSQLYAVADTLVKTGFN
                     ITADHSGLGGGAKEDNVKLGETVKYTSTDKNVVTTVTNNTIDFGLSDNISVGGKPGKD
                     GSVGVKGADGKDGVTIKPEAIVFHGVDGVNGKDGKDGKASIKVEKGAKGLDGNDGKDG
                     ESKTRIVYEKPNGDKEEVATLNDGLKFGANDGKVHDAKLNTQVDIKGATTNTDWAKFD
                     AGKNIMTQVVGNTITVALAKDLAGLDSATFGTQGDSTVINKDGVTIAKKDPNDATKSN
                     VTLTEDGLSNGGNQITNVDSGLKGKDGQPVKLTEAAGDVLNNAVNVGDLKNTVTETVT
                     EVTTDAFGLKDENNKEFKQNLGTTAKIIGDGNVKTNVVEKDGGHALEVSLVNNITVGK
                     DGEPGKVGIAGKDGKDAIALDGKDGAITVGRPGKDGIDGTIGVNGKDGSAVVLNGKDG
                     SIGLNGTNGKDGLTIKGTEGKPGLVGKDGLPGKDGVTRIQYQPTDGNGDPIGEPEQVA
                     TLNDGLHFKGDDATLVSKKLNETLEIIGGADKDKLTANNIGVNSKDGKLSVQLAKDLK
                     DLNSATFKPQDGENGQEGKGPTTVVNNNGVTITPKDGTDGKAVKLTEDGLDNGGNQIV
                     NVKSGIDGLAGKDGINGKDGKPITSVNDLTDDQLKEVGTNAANINDVVNASNDIIEKG
                     FSIAADNGETDHVKLGETVKYTSKDKNIITTVSDNVIDFTLNNDFTIGGPGKDGSIGV
                     KGADGTVGVTLNGKDGSIGLTGPKGADGKDGASATISVKDGAKGLDGNDGKDGESKTR
                     IVYEKPDGTTEEVATLNDGLRFVGDDGKEIVKKLNETLKISGGDTDLTNLSDNNIGVV
                     NNNGALTVKLAKDIDLTKDGSVTIGDTTVNNDGLTIKDGPSVTKAGINAGDKKIINVQ
                     DGEISATSKDAVNGSQLYQVQEVANAGWNLTANGKDKGNVKPGATVDLNNKDGNIIIT
                     KEGNNVTFGLKNNLTIGGKDGKDGQIGIAGKDGKDGVTIKGDGTITAGRDGKDGVDGS
                     IGATGKDGASVVLNGKDGSIGLTGPKGADGKDGASANIAVKDGAEGVDGTNGKDGLPG
                     ENGKTRIVYETKDKDGKTVTEQVATLNDGLIFSGNNEDVKNRQKLNTEVKVKGEGVDK
                     TASENFKSASGNINVKANGTDTLEVQLAKAIDLTEDGSVTTGDTTVNNNGLTIKDGPS
                     VTKGGIDAKGTKITNVKDGDVTATSKDVVSGSQLYNAMQNTGWYLTVDGNVAETSEKG
                     AQRVNNNGKVAVSGGKNIVVSRKGSTVEVATSSNLEFDSVKVGGTTISSTVAKDGVNE
                     LNIAGSKNAQTRITNVAPGVKGTDAVNVNQLKGAENRLNNRISNVDKDLRAGIAGALA
                     AGNLYHVTQPGKSMVSAGVGAYRGQGAIAVGYSRLSDNGKVGIKFSVNSNSRGDTGAA
                     ASVGYQW"
BASE COUNT         8897 a         6788 c         5977 g         8819 t
ORIGIN      
        1 ggcaatgtca aatatcggtt tagataaaga aatcgcaaaa aaattagcag atgagttaaa
       61 tggtttatta gctacttacc aagtgtttta cactaatgta cgtggctacc attggaatgt
      121 acgtggtgtg aacttctttg aattacatgc aaaatttgaa gaaatctacg atgatttagt
      181 gacaaaagta gatgaaattg cagaacgtat tttaacctta ggtcacacac ctcaaaatgc
      241 gttcagccaa tatttaacca agtcacaaat tcaagaacat attggtgttt cagctgcaca
      301 agaatgttta cgtggtactt taaatggttt caaaacatta ctgggacaac aacgtgaaat
      361 tcttgctcta gcaaatgatg caaacgatga aggtactgct tctcaaatga gtgattacat
      421 caaagaacaa gaaaaattag tatggatgtt cactgcagct tgtgctgaat gttcagacta
      481 cgcacaaggt tgttcttgta actaagttgc aataatataa ttaacccccg ataatgaaag
      541 attatcgggg gttacttttt cagatagcta tgtcgcaagg atttcaattt aaacagtttt
      601 ttattcggca cgacagatgt gcaatgaaag tcaatacaga tggtattcta ttgggagcga
      661 ttgctcacat aaaagattgt cagaatattt tagatctggg tataggttct ggtttagtag
      721 cacttatgct tgctcagaga acagatgatt gttgtcacat tactgcgttg gaacttgaac
      781 caaatgccta ccaacaagcg gtcgaaaatg cccaaaattc tgcatgggca aatcggattt
      841 ccgttttgca aggtgatgtg atgcagcagg catttgaaca gaaatttgac ttgattgtta
      901 gtaacccacc ttattttgca gattcacttg cagctcgaac cacagagaga gatttagctc
      961 gctcaattag ccattacagc cacttgaatt ggctggcaaa agcaaaacca tggttagcag
     1021 agaacgggaa aattacactg attttaccta tagcagctgc agaaaaatta gttgaccaaa
     1081 gtgaacaaat tggattgtat tgtgttgaac aatggttaat ttttaccaag agcggcaaat
     1141 tggctaaacg cagtatagtg agttttagtt taaacaagca aaagcgtgaa cataaagagc
     1201 tgatcatcta tgatctagat aattgttaca ctactgagtt taaaagactt acccaagatt
     1261 tttatttgaa tttttaattt actccccggg ttagtgtttt ccacaaatct tcttcagcta
     1321 cgatgatcta gccttaagct aaaaagcaga ttaccaccca agataatccg ctttttagac
     1381 ttagctatta tgccaaactt gctaccatta ccgctttaat agtgtgcatt cgattttctg
     1441 cttgctcgaa tgccacattc attggcgatt caaacacctc ttcggttact tcaatgccgt
     1501 ttgccagtgc cggatatttt tcggcgatct ctttaccgac tttagtttca ctgttgtgga
     1561 acgccggtaa gcagtgcata aatttcactt tcgggttgtc ggttgcttgc attaattcag
     1621 gcgttactcg gtaaggcatt aataaatcta tacgttcagt ccagctctct agcggttcac
     1681 ccattgaaac ccaaacatca gtatgcacaa aatcgacacc tttaattgca gtctcaatat
     1741 cttcggttac cgtcattctt gcccccgatt cagctgcaaa tttttcacac attttcacga
     1801 atgcatcttc cggtaataag gctttcgggg cacaaattcg cacgtccatc cccaatttcg
     1861 ccccaatcaa caggagtgaa ttacccatat tattgcgagc atcgccgata tagacataac
     1921 tgatttggct aagcggtttt tcgcaatttt cgatcatcgt tagcacgtca gccaacattt
     1981 gggttgggtg gaattcatcg gttaagccgt taaacaccgg cacgccggca taatcggcaa
     2041 gctcttgtac taccgattgt ttaaagccgc gatattgaat cgcatcatac attctgccta
     2101 gcactcgggc ggtatctttc attgattctt tatgcccgat ttgcgaggag ttcgggtcaa
     2161 tataggtaac gtgagcccct tgatcgtaag ctgctacttc aaaggcacaa cgagtacggg
     2221 tcgaggtttt ctcaaaaatt aaggcaatat ttttgccctt taatttttgc tgctcagtgc
     2281 cggcatattt cgcccgtttt aaatctcttg ctaaatccaa caaaaagcga atttcacgct
     2341 cagtgtggtt tactaaactg agtaaatgtc tgttttttaa gttaaatgcc attttgtttc
     2401 tccctgtaaa cattaaaatt tattttatgc aaaataattg cataaaaatt cacttatagc
     2461 aaacagaatt tgcttaccct cattcgggta aataaaactc gcctttgccg actaaaataa
     2521 cctctccgcc aacaaaaatc ttcccgtttt gatcgacttt taataacagg cgattcggcc
     2581 gttgaatttc atcaccttgt gagatctgaa gctggattgg tattttgcct tgtgcaatgt
     2641 gccaaccgcc taaatttgcc gctgccgaac cggtgccggg gtcttcgaca atcgaacctt
     2701 gcgattgaaa aaacagtcgc actttcgcct catttccctc acgacaccaa acatatacac
     2761 tgtctttcaa aaatgctttt tcagccagat ttgcatttat tttgcacttt tcgaccgctt
     2821 gttgagaagc taactcaacc aataactgct ccacgccggt gtttacataa caaggctcgg
     2881 acgcaatatc cgcaacattc agccccaata ttgaggcaca ttctgcacgg cttagcggtg
     2941 tgttttccag gttcacacca ttggttaaag caaaagtagt cacattatgt tgatgttgaa
     3001 tcgctaccaa tccggcatta gtttgcaagc gataggttgt tggaaaatca aaggcggaat
     3061 gcaacacaaa agccgctcca acagttgggt ggccggcgaa cggcatttca taatcaggcg
     3121 taaaaatttt gagttttttg accgcttgtg aatcggtgga agattgaata aataccactt
     3181 cagaaaggtt aaattggcga gcaataagtt gcatttgtaa gtcggttaat ccgtccgctt
     3241 cataaaatac tgcaagcggg ttgccgccaa aatggcttgt tgcaaacaca ttcaccagca
     3301 aataaggata gctctgcatt tatcactcct tacataaact taaagccaca ttgggcgaaa
     3361 attctaccat tttggtggcg aatcgtaaaa ttggaaaatt tatcggtatc cctctttaga
     3421 gttaactatt tttgatttat ctcaaagttt taaaaaaaag attaggtcat acttctttct
     3481 agtgattatt ttttaatttg aggttcttat gctttatgca gaatttttac ttttattagc
     3541 ctttttatat gcaggtagcc gttacggtgg catcggttta ggtgtggttt ccggcatcgg
     3601 cttatttgtt gaagtgttct tcttaggaat gccgctttcc gctccgccaa ttagcgtgat
     3661 gctcgtgatt ttggctgtag ttacttgtgc ctccattcta gaagccgctg gcggtttgaa
     3721 atttatgttg caagttgccg aacgcatttt gcgtagcaac ccaaaacgta ttacctttct
     3781 tggcccactt gtcacttatc tgatgacctt aatgctcggc acagggcatt cggtttactc
     3841 tattatgccg attatcggtg atattgcctt aaaaaataaa attcgcccgg aacgcccgat
     3901 ggcagcagcc tctgttgctt cacaattagg tattaccggt agcccacttt ctgctgcagt
     3961 tgcctattac ttaaccgaaa tcgttaaatt accgggcttt gaaggggtga ctttattgaa
     4021 tgtagtcggc gtaaccatca ccgcaacctt ctgtggtgtg attgcgatgt cattatatag
     4081 cttacgtcgt ggtaaagagt tggaagacga tcctgagtat caacgccgta tgcaagaccc
     4141 tgagctacgc aaacaaatcg agcaaacgag tgcaacgtct ttagacgaaa aactaccggc
     4201 aagtgcgaaa aactcggttt atctgttcct agccgctatt gcaaccattg tgattattgc
     4261 aatgattccg gctattaaac caacggttgc tgctacagga aaagttgccg gtatggatca
     4321 aatcattcaa atcgtgatgc taactttcgg gggtttaatc ttaattttaa ctaaaaccga
     4381 cccgaaaaaa gtaccaaacg gcatcgtttt caaatccggt atggtagcaa ccattgcgat
     4441 tttcgggatt gcttggatga gtgatactta cttcacccat gcaatgccag cgttcaaaga
     4501 aggggtaaca gaaatggttc gagcccaacc ttggacattc gccttcgcct tgtttgcggt
     4561 atctgtagta gtaaacagcc aagccgtaac cgcaaaaatg ttattaccgg taggcattgc
     4621 aatgggcttg cctgctccat tattagttgg tttaatgccg gcaacttatg cttacttctt
     4681 tattccgaat tatccgtctg atatcgcaac cgttaacttt gacgtaaccg gtacaactaa
     4741 aatcggtaaa tattacttca accacagctt tatggctccg ggtttagttg gcgtaattac
     4801 tgcctgtatt gtcggtatcg ccgttgcaaa tgtgctaatt taattttaac aaaaaaacaa
     4861 gcggtcagat taagtaaatt ttttaccaaa tctgaccgct tgattattta ctgcaaaacc
     4921 aattaggctt tgttaaagag ttttgcatct aacgagtatt ttccgccacc gataaaaata
     4981 taggttaaga ataataccga atacacgcct gctaattcac cgccgtttaa taaaggtaat
     5041 agcacattgc cttgagctcc gtgagcaata aagaacgcgt atgccatcat acctgaaagg
     5101 ataaacgctg tttggcgggt aaatagacct aaaataaaga aaattccgcc cacaatttcc
     5161 aaaataccgc ctacaccata aatggaggca aggggtacac tgccgtttcc gttggtcatc
     5221 gacattggaa attcaaaaaa tttcgctgtg ccgtgtaata aaaacatata accggcaaca
     5281 acacgggtga ttaaaaacgc taaaggttgt aaatttgtta aagtagagtt catttgcttt
     5341 ccttaaaata aattgtgata taaaggtcaa ttgattgagg caggcattct attttaaaaa
     5401 cctatacttc acaatccagc cgtcatgaaa tagattattt accattaata aacaattagg
     5461 ctctcgctaa cacccaaacc tgaatttcta ctactccagc tttgagtaac tcggcacaaa
     5521 ttacatttaa ggtggaacct gtggtgagca catcatcaac aatcgccact cgtttatagg
     5581 cttttaacgg ttgatagaaa aatgccccac gcagattttt ccgccgttca gcagcggtca
     5641 attctctttg tgagacggtt gctctcacac gttgtaaact ctgggtatcc agcggaatct
     5701 gtaaccactt cgataaccaa tcacacaata attcgctttg attaaaccct cttttccaat
     5761 gtctgtgcca aaaaagtggt acaggcataa tcacttcagg caagctcaga acctgttccc
     5821 tttgagcttg tttgattgcc agcagcaatt gtcgggcaag ggcttgatcc caccaatatt
     5881 gctgctggaa cttaaattga tgtacccaat gtgaaagtgg cgctcggtat tcatttaccc
     5941 tcactaattt atgccacttc ggttcatttt tcaaacaata gccacaagcg tttcgaaact
     6001 ccaccgcact cattccacaa tgacgacaat aaggggttgg ttttagcaat ttttggcaat
     6061 ggctgcaaat gccttgcacg ccaatagcga gtggatgatt acaatgaaag caacggaagc
     6121 cgaataaatt cacatttcct ccttttattt gcagattatc aaatttagcc ttattttttc
     6181 cggctatttt tgtgacatcg ctcgaaaaaa gagtaaaatc tcattatcag taaataaaaa
     6241 ggaaaccgag atgaaatccc tccaaaaacc gatcaaaatt gtctttttcg atattgatga
     6301 caccctctat gtaaaacata aagcctatat tccaaaatca attaccgagc aggtattacc
     6361 tcgtttaaaa gccaaaggca ttattccggc cattgccacc ggcagaaatc acggctgttt
     6421 tcctgaagct ctcaaacctt atttaaaccc tgaaatcggt tttgagctgt ttgttaccat
     6481 taacgggcaa tataatttct ataaagatca aataattagt gattattcgt taagccgtga
     6541 gcaaattgaa cgtattatcg aaaaagcgaa tgctttgggt attctgtatg cctttattac
     6601 gccaatgacg attgcagttt ccgaaaccaa tccgattgtg cacgaagcgg ttttaccgat
     6661 cacaccgaat tatttgatcg atccacacta ctacgaaaaa gagaaaattg tgcaaatgtt
     6721 actgttctac accgaagagc agaaacaagc ggtcgaaaat agtggcattt ttgcaaatga
     6781 tttaaaagag gctcgctggc accccaatgc ggtagatatt ctgcgaaaag aaaattcgaa
     6841 agctcgtggc attcaagatg tattagcaac cttgaatatt gagattgaaa gtgcgatggc
     6901 atttggcgat ggtattaacg acttggaaat gctcccgacc gtaggtgttg gtgtggcaat
     6961 gggtaatggc gtgccggaat taaaagcggt ggcagatttc atcaccaagc cgcttgagga
     7021 agacggcatt ctatacgctc ttgaaactct tgaggtgatt taaaagcaag cggttgtaag
     7081 tttttctcca tttacaaccg cttgtagttt ctaattacgc ctaaatagct tatcgcctgc
     7141 aatcgctaaa atcgccccac tgacgaccac caacgcccca agataactga tccaattcat
     7201 atccggcttg gcaaaaatat gcggataaaa atagtagcta aggttggaaa agatcatcgt
     7261 aaaaatcggc aacatcgtgg ttacaatgct aacctttgaa gtatcccaat gattcaacgc
     7321 ctcaccgtat gaaccgtagc caattaaggt gttcaacagc aataaataaa gcaaccccac
     7381 agaaaaccat cctcaagatt gcctaactgt gccggtgagg caaacggtaa catcagcaga
     7441 gaacagccga tgtaaataat aaacagcact tgctgagagt taaatttcgc taataataat
     7501 ttttgagcca cgccgtatag cacccaaatc aagctggctc cggcactcaa taacactccg
     7561 agagcgtagg tgttgagctg taaaatttca gcaaatttat cgttaaaaaa tgcgacaagc
     7621 cctgcaatca ataacacaaa tccaatcttt tgaaacaagc cgaacttttc cttaaacaat
     7681 aatacgccta gtagaatcat cgaaaatgga gctaactgcc acaatacttg gttaatggtg
     7741 ggcgaaacat agtgtagagc ctctgcaaac aaaaagaaat ttgccgacag accacacacc
     7801 cctaacaaaa ttaaaccgag ttcttttttg ctacaagcgg tcaatttggg taaacgtttt
     7861 gcaaatccta gaatcagcaa tagccctact gatgccacaa cgaacctaac ccaaactaaa
     7921 gtttgggcat ctatcacttt caatacttgc tgggcggcaa tcggtagcgt tccccaggtc
     7981 attgctgcgg ttaatgcaag tgaaaagcca agtaaggggc gttgtttcat cgtggaaatt
     8041 cctttttcta ttctatgttt ctatatttta gaaagattga gatacaaaaa aaccccgcca
     8101 ttggcgaggt ctcttattga ttttgaaatg attagtcagc aatgatgctt acatatttac
     8161 ggcttttctc gcctttcact tcaaatttaa ctttaccgtc agcagttgca aataaagtgt
     8221 ggtctttacc catacctaca tttgaacctg cgtggaattt agtaccacgt tgacgaacga
     8281 taatgctacc tgctaataca gattcaccac cgaaacgttt aacaccaagg cgtttagctt
     8341 cagaatcacg accgttacga gttgaaccac cagctttttt agttgccatc ttctagatct
     8401 cctctgaaat tatgcttgaa tcccagtgat tttcacttct gtgaaccact gacgatgacc
     8461 ttgttgttta cggctgtgtt tacgacgacg gaacttaacg attttaactt tctcgccacg
     8521 accttgtgct acaacttcag ccactacttt tgcacctgct acaaccggtg caccgatttt
     8581 aacatcttca ccgttaacga ccattaacac tgagtcaaat tcaactttct caccagttgc
     8641 aagttcaagt ttttctaaac gaactacttg accttcactt actcggtgtt gtttgccgcc
     8701 actttggaaa actgcgtaca tatttactcc gctatgtgcc aactgctttt gcttttaggc
     8761 actaaattaa ttcataaaaa tagggttgca attctacgga aaaagattcg aaagagcaag
     8821 agcgattttg agaatttttt gaaatttgca aaaaatggta aaaatactac cgcttgtttc
     8881 aactgcgact aaaaaacaat agactgctcc gattttacaa caaaatcata attctttttt
     8941 gaacacaggc taaattaagg tagaatctac tcaattttcg tcttacttta tggattcgat
     9001 ggctgtgcaa cttttttctt tagaacaaat tcaagccctt gcgaatgaag atatgcaagc
     9061 ggtaaatgcc gaaattttag ctcaacttaa ttccgaagtg ctactgatta accagctcgg
     9121 acattatatc atcgcaggcg gtgggaaacg tattcgcccg ttaattgcgg tgcttgcggc
     9181 tcgtgccttg ggttatcgtg gtaaaaagca tatcacggcg gctgctttta ttgagtttgt
     9241 gcatactgct acacttttgc acgatgatgt agtcgatgaa tccgatatgc gtcgtggcag
     9301 accaaccgca aacgcccatt ttggcaatgc ggctagtgta ttggtagggg atttcatcta
     9361 tacccgttcg ttccagatga tgacggattt agactctctg cgtgtgttaa aagtgatgtc
     9421 tgccgccacc aatgcgttgg cagaagggga agttcagcaa ttaatgaacg tgaacgaccc
     9481 aaacaccact gaagcgaact atatgcgggt catttacaat aaaaccgccc gcttgtttga
     9541 agccgccact caatgcgtgg cgattgcggc tgatgcttct gaggaaattg aaaccgcatt
     9601 aaaaaattac ggttgctact taggcacggc attccagctg attgacgata ttttagacta
     9661 ctccgctcaa agcgaaagtt taggcaaaaa cgtaggcgat gactttgtgg aaggtaagcc
     9721 aaccttgcca ttgctccacg caatgcacgc tgccgacaaa gcaggcaaaa ctactcaagc
     9781 agagctgatt cgcaatgcta tcgaaaacgg cggcgaattg gaacaaattc cacaagtatt
     9841 ggcaattatg caagaacatc aatctctgga ttatgccatg caaaaagccc gtgaagaagc
     9901 ccaaaaagcg gtggattcat taggggtttt accggaaagc gagtacaaac aagccttggt
     9961 ttcattggcg tatttatcgg tggatcgctc ttattaattt gcaaaaaatc atataaattt
    10021 aaccacttgt aggggcaaat gatatttgcc ccataaccta taattctttt gggcaaatgt
    10081 tatttgcccc aacataacaa tgactgaaca aaaccaacga ccccaagaac atattgaaag
    10141 cgtacgagcg agacgccgtg ctcaaaatca gaaaaaaggc ccgaatgccc cgtttgtgcg
    10201 tgagaaatta acgctgccaa acggacataa caaattactg ctgcactctt gctgtgcccc
    10261 ttgctcgggt gaggtaatgg aggctattca cgcctccggc attgagttta cgatctattt
    10321 ttataacccg aacattcacc cgctgaaaga gtatttgatc cgcaaggaag agaatattcg
    10381 ctttgccgaa aagtggggta ttccgtttat cgatttagac gatgactacg aaaacgaccg
    10441 caaagagtgg ttcaaaaaag ccaaaggaat ggaatgggag ccggaacgag gcattcgttg
    10501 cacaatgtgc tttgatatgc ggtttgaaaa agcagcggaa tacgctcaca acaacggttt
    10561 ccctgtattt accagttgct taggcatttc ccgatggaaa gatatgaacc aaatcaacga
    10621 ctgtggtcac cgtgcagcag ccaaatttga tgacgtggtt tactgggact acaactggcg
    10681 taaaggcggc ggctcacaac gaatgatcga aatcagcaaa cgtgaacgtt tctaccagca
    10741 agaatactgc ggttgcgtct attcgttaag agatagcaac aaatggcggt tggaaacagg
    10801 gcgtcagcgg attgaaatcg gtaagctcta ttactcaccc gattaaatgt tacttttctt
    10861 tgcttcgcca aagaaaagta accaaaagaa aggcgacccg aacttcgctt agttacgatt
    10921 tccttaacgg aaaatttgta aactcgctac gctcaaacag tacaaatttt cctagaaatc
    10981 gcaagtcgct cagacgggac aagcggtaca ttattcaaaa ttttttacaa aatatagtga
    11041 tgtactcttc ccttctgaac cgcccgaacg tgtttgaatt ggtaggaaaa tttactctga
    11101 gcgaagcgaa tgtaaatttt ccgtgaaacc aattcaaact aagtgaggat aagcggtgaa
    11161 gtaggggtgt ttctttggtt actttctttg cacatgcaaa gaaagtaacg aaaactcaga
    11221 tatagatttc aacttaaaaa tttacagaag gaataataac aaatgtcaaa attcgctatg
    11281 gttttccccg gtcaaggctc acaagcggtt ggaatgcttg ccgaccttgc gccacaatat
    11341 ccaatcgtgg aagaaacctt caaacaagca agtgatgtgc ttggctatga cttatgggat
    11401 ttagtgcaaa acggtactgc cgaagagtta gggcaaaccc accgcactca acctgcgttg
    11461 cttgcctcaa gcgtggcact tttccgcatt tggcaacaaa aattccctga gcaaaaacca
    11521 gccgtgatgg cagggcatag tttgggtgaa tactcagcgt tggtgtgtgc cggtgtgttg
    11581 gatttccaag atgcgattaa attggtcgag ttgcgtggtc gtgcaatgca agaagccgtg
    11641 ccggcaggca gcggtgcgat gtttgcgatt atcggtttag ataacgaaag cattattaaa
    11701 gcgtgcgagc aagcagcaag cgagaccggt gaaattgtct ctgcggtaaa ctttaacagc
    11761 ccggggcaag ttgtgattgc cggcacaaaa gcagcggcag aaaaagcagg agaattgtgt
    11821 aaagccgcag gtgcaaaacg tgcgttacca ttagcggtga gcgtaccttc acactgtgct
    11881 ttaatgaaac cggcggcaga aaaattagcc gaagccttgc aaaatattga gctaaatcaa
    11941 ccgcttgtcc ctgttatcaa caacgtggat gtggcagtgg aaacagatgc cgataccatt
    12001 cgtaatgcac ttgttcgcca actttacagc cctgttcgtt ggacggaaac cgttgaaaaa
    12061 atggcacaag atggcatcac tactttatat gaaatcgggc caaacaaagt cctaaccggt
    12121 ttagcaggtc gtattgtaaa agaattatca gccaaagcgg taaatgatgt tgccagcttt
    12181 gaggcggttg aggcttaatt ttcgggagcg taaaatgagt aaagaaaccc tatttatgct
    12241 aataagtgca ttgattgcct tagtgatttt acctttagct tatactgttg gtatcgctaa
    12301 agaaacatta ggcggaaata tgggaatgtt atccattgct gccggttgct catttttagc
    12361 ctatttttta agtctaaacc cgaaaattcg ccacgccttt atcggaggtt cagctattac
    12421 agctgctgct attcaagctt gggtattata tggtgtgcta ttaagcgaaa atgaccccaa
    12481 aagtgcggtc gttttatttc aaatttatct gtttagtagt ttagggccac tgtttttttc
    12541 agctatttgg gcatttactc gcccacgttg gatttaccct aaacaaggta gtgcggtgaa
    12601 atatgcctta tttggtggct taacgaccgt gataaactac ttgattaccc tttttatttt
    12661 ttatattctg acgttataac gtcatactaa ggagaattta tgagcaaaat cgcattagtt
    12721 acaggggcaa cccgtggaat cggcaaagcg attgccgaag aattggtagc aaaaggctac
    12781 atcgtagtcg gcaccgcaac ctctgaaaaa ggggcggaat ctatttcagc ttatttaggc
    12841 gagaacggca aaggcttagt gctaaatgtt gccgatgcag agtcgattga gagcgtatta
    12901 aagcagatca aagagacctt tggcgatgtg gacgtgttag tcaataacgc cggcattacc
    12961 cgtgacggct tattaatgcg tatgaaagat gaggattggt tcgacatcat tcaaactaac
    13021 ttaacttcgg tttatcgttt atccaaagcg gtattacgcc cgatgatgaa aaaaggcgga
    13081 cgcattatca cgatcggttc tgtggtcggc tcaatgggca acccggggca aaccaactac
    13141 tgtgctgcca aagcaggctt agttggtttt tctaaatctt tagcgaaaga agtagcaagc
    13201 cgcggcatta cggtaaacgt ggttgctccg ggctttatcg ccactgatat gaccgatgag
    13261 ctaaccgaag atcagaaaaa tgcgatttta agccaaattc ctgccggtga actcggttct
    13321 ccacaagata ttgccaaagc agtcgctttc ttagccagcg atgatgcccg ttatatcaac
    13381 ggtgaaacca ttcacgttaa tggcggttta tatatgagct aatccgctac aagcggtctt
    13441 ttttcaaccg agctttgtaa attcagagtt cggttttgtt atttgttatt ttgtgttatc
    13501 acctgcttta taggtatagg tgaaaaagtg tgaatatgtt atgactagaa atacattagt
    13561 agtaggcttt ttattgtttg ctattttctt tggtgccggc aatctgattt tcccaccaaa
    13621 attaggttat gaaagtagta cagaattttt ctcagccatt tcaggctttg ttttaactgg
    13681 agtagggctt cctctactcg gtattatcgt cagtgcattt tataatggag gctataaatc
    13741 tgcattagac aaaatccacc cttggttttc tttagcattc ctaacttcta tctatttagc
    13801 gatcggtcca ttctttgccg gtccgcgcac ggcagcaact gcctatgaaa tggcgataat
    13861 gccgttttta ggggattcca gccaagtgtc aatgttaatt tttaccttaa tttactttgc
    13921 ctttacccta tggttagcgc taaatccaaa taaaatggtt gatcgcattg gtgcaattct
    13981 taccccgatc ttgcttataa ccatcattgg attagttatt cgagccggct ttattttaga
    14041 taccgatcca caaatcacta cgcctaatac gaatgaacaa tcttactttt ttaaaggtat
    14101 ggttgatggc tatctaacta tggatgcact tgcttcgtta gcctattcgg taattgtatt
    14161 aaatgctatt cgtgggaaag gggtaagcca agccgcatta caacgccaaa cagtttctgc
    14221 cggcttagtt gcagcagttg ctctagctgt aatttatatt tcattgggtt ggattggtaa
    14281 taatatgcca attagtgcaa acacactttc gatgttatca gaaagaaaat tggatctagg
    14341 tacttacatt cttaacactg tgactaccca agccttcggt gaactcggac gtagtgtctt
    14401 aggagtgatt gtttcacttg cctgtttaac cactgctgtc ggtttagccg tatcagtcag
    14461 tgaatacttt aatgaaattt tcccgaaaat ttcatataaa gtttatgcta tcttatttac
    14521 tgtgattagt tttactgttg ctaaccaagg attaaaagaa gttattagca aatccattcc
    14581 ggtattatta gttctctacc caatagcaat gactgtcatt ttaatgttgc tgattaattt
    14641 agtgattaaa cttccgttgt ctgcacaacg tctatcactc ctattagtga caattgtagc
    14701 gatagcttca gttgccggtg tcgggtttac cgaaaacttg cctctaaaat cctactcgat
    14761 ggaatggcta cctttcgcta tcattggagc tattattgga gcaattatcc acccgcttac
    14821 cggtcgaaag cataaataaa gtaaaggcag tgagcattca ctgcctcttt tttaggagag
    14881 aataatgcct gaattacccg aagttgaaac cagcgttcgt ggtgtatcgc cctatttggt
    14941 tgggcaaact attgaacaaa tcatcgttcg tcagccaaag ttacgttgga cggtgagtcc
    15001 tgaattaagc caaatggcag gggcaaaaat taccgcagta gaaagacgtg ccaaatatct
    15061 gattatccgc accgacaaag gcgatatttt ggtgcatctg ggaatgtccg gctcgctcgg
    15121 gattttgcaa gaacgtcaac aaaaagaaat tggcaaacac gaccacgtcg atcttattac
    15181 ccaaaacggc acaattttac gctataacga ccctcgcaaa ttcggctgtt ggctatgggc
    15241 agaaaaagca gaagatcacg aactgttgcg taaactcggg cctgaaccgc tttcaaacga
    15301 tttcacagca gattacttat ttgcaaaaag ccgcaaaaaa acgaccgctt gtaaaacctt
    15361 tattatgacc aatgaagtgg ttgttggggt ggggaatatc tatgcgtgcg aaagtttatt
    15421 tatggcaggc attcaccctg agcttgcagc acaaaacctg actaaaaaac agtgcgaacg
    15481 tttagtaaaa accatcaaag aagtgctgac taaagcgatt attcaaggcg gcacaacact
    15541 gaaagatttt atccaacctg acggcaaacc aggctatttt gctcaagtgt tgcaagtgta
    15601 tggaagaaaa ggggaaacct gtaatgactg cggaaagccg attgaaacta aagtaatcgg
    15661 gcaacgtaat acctattttt gcccgaagtg tcagagattg ccgaagtaat ctaataataa
    15721 actctctccc cctgagggag agagacagct tgttgaaacg ttaagtgaaa caagcagaga
    15781 gagggtaaaa cctaaacctg caaccaaaag gtaacaggcc cgtcattttg taggctgact
    15841 tgcatatcgg ctgcaaattg cccggtttgg gtatgaatct tagtggcggc ttgttgatga
    15901 aagtaggcat acaaacgttc ggcatcaacc gggcttgctc cttttgagaa actaggacgc
    15961 aagcctttat tggtatccgc cgcaagggta aactgtgaaa ccactaacaa actgccacct
    16021 gcttgctgta cgtttaagtt cattttcccc tcgctatccg aaaacacccg ataattcagc
    16081 actttttcca gtagcttatc tgctttcgct tcatcatcgt ctttttccac accgagcagc
    16141 accaacaaac cgcttgcaat ttcgccaacg acttgccctt caacctccac ttttgcgaat
    16201 ttaacccgct gaattaagcc tatcattaat gctccccttg tctgtttcca tttcgtctaa
    16261 aaccgccgca aattgcccgc caagtaatac cacctgccaa cttaactgaa tccataacag
    16321 cataatcggt aacaccgcta atgctccata aattgcctga taagacggga aggcagtaat
    16381 ataccaaata aaggctttct tccctaaagt aaagaaaatc gctgccatta atgccccgat
    16441 agcagcgtgt tgaaatttta cctttctaat tggaataaat ttatacacta aggtaaaaag
    16501 tagccaaatt agcaaaaacg gcatcaattc taataaatgc tgcccgagcg ataacgctcc
    16561 atttccatta aaaatagaaa gcgacattac ataggtgcta atcgcaatgc tagcaccggc
    16621 aagcagcggg ccaaacagca aaatgagaaa gtaaagcaag aaagagaacc acatcggacg
    16681 ctgacgatta tcgtgccaaa tggtattcaa tgcctcatca atactcttaa tcagcataat
    16741 agtaaccaca aataagccga taaaacttac cagccccatt tgtttggcat tactgacaaa
    16801 caactcaatg tattccgaca ccatctcgct cgcactcggg gcaaagttgt cataaatgaa
    16861 cgcttttaac tgctcgtttg cttcgtggaa aatcggaaaa aaggtaaaaa tggaaaacag
    16921 cacgacaata aacggcacta tcgccagcat tgtgctataa gtgagataac cggctgaaat
    16981 cgcaatgtta ttttcattgt aacgcttggc aaagagctta ataaatctcc acagtcgatt
    17041 taacatctta acctctcacc aaataatcgc cttcacacac ccatttgtag gtggttaagg
    17101 cttctaatcc catcgggcct cgtgcgtgta atttctgcgt gctaactgcc acttctgccc
    17161 ctaaaccaaa ctcaccacca tcggtaaaac gggtgctggc attaatataa accgccgcgg
    17221 catcaacctg ttgtacaaat tgacgggcaa gttgataatc ggaggttaaa atcccctctg
    17281 aatgctgcga accataatgg cggatatgct ctaccgcttg ttgcaggtca ttcaccacca
    17341 ccacgttaag atcaagcgat aaccactctt ggctcaattt tgcttgatca agcggttcaa
    17401 ttccttcaat tttttgcaaa tcgttactgt gaatagtaac gccaagctgt tgcattttgg
    17461 cgaccaattt cggcaaaaat tccaccgcaa tcgctttttc caccaacagc gtttccagcg
    17521 tattgcaagt gcttgggcgt tgcgttttgg cattagcgat gacctctaga gatttctcct
    17581 gatccgctgt tttttccaca aacagatgac aaaccccaat cccccccaca atcaccggaa
    17641 tggtcgaatt ttctttacag aattgatgta aacctgcacc gcctcgaggg atcaccatat
    17701 caatgtagcg atctagcttc aatagttcca ataataaggc tcgatccgga tccgtcaccg
    17761 cttgcactgc caatttcggc aagccggctt tttctaacgc atcttgcact acatcaacca
    17821 acacagcatt ggtaaatttg gtctctttac cgccacgcaa aatcactgca ttgccggttt
    17881 ttaggcataa tgatgccaca tcaatggtca cattcgggcg agcttcgtaa atggttaaaa
    17941 tcaccccgag cggcacacgc tggcgttcga ttttcaaacc gctgtctaac acgccaccgt
    18001 ccatcacctg ccccaccgga tccgctaatt tggcaacatt tcgcacatcg ttagcaatgt
    18061 cttttagccg atcttcattc aataataagc ggtcaataat cgccaatgaa atcccttgtg
    18121 cttgagcaaa ttcaatatct ttggcattag cgtataaaat ttcttctgca cgttgctcaa
    18181 ggctatcggc aatcgtgagt aaggcttggt ttttctgtag gttgccaaat tgagccagct
    18241 caaaactagc ttgtttggct tgctgggcaa gttctatcat ctgcataaat taggtctcat
    18301 ttaatcaata ttctccataa tctaacacgt tcttttatgt gaagaaatat gcaaaagcca
    18361 agaaaattgg ataaaatggg ggaattttaa aacattacgc aattcccctc tccctgattt
    18421 ttctcctgaa cggagaaaaa tctatccctc tcctacaagg ggagagggta gaatttaact
    18481 ctctcccctt gtgggagaga gaccgcctac agattcgttc agaatctgta ggcagagaga
    18541 ggggtaaaaa cagataaaac ttaaacaaaa tgataaacac tacccctaaa cagctaatcc
    18601 acattcttcc gcctcaactg gcaaaccaaa ttgctgccgg cgaggtggtg gaacgccctg
    18661 cgtctgtggt aaaggagcta gtggaaaaca gtttagatgc cggagccaat actattcaaa
    18721 tcgacattga aaaaggcggc tctcagctta tccgcattcg agataacggt tgtggtatca
    18781 gcaagcaaga tttaccgctg gcacttgctc gccacgctac gagcaaaatc agcagcttgg
    18841 aagatttaga agccatttta agtttaggct ttcggggcga agcattggca agtatcagct
    18901 cggtttcacg tttaacactg acctctcgta ccgcagagca aaccgaagca tggcaagcct
    18961 atgcacaagg gcgggaaatg gcggtggaaa ttcagcctgc ctcacacccg attggcacaa
    19021 ccattgaagt agcaaatctg ttttttaaca caccggcacg gcgtaaattt ctacgcaccg
    19081 ataaaaccga atttgcccat attgacgagg tggttcgccg cattgccctt gccaagccga
    19141 atatcagttt cagcttaagc cataatggta aaatcgtccg ccaatatcgc aaagtgcaag
    19201 acaattcggt agagcagcaa caaaaacggg ttgcagccat ttgcggtgat gagtttattc
    19261 aacattcaac ttatattgac aggcaacacg gggatctgca cctacacggc tgggttggct
    19321 caccggctct tgcccgtctg caaaacgacc tttgctacag ctatgtgaac gggcgaatga
    19381 tgcgggataa aaccatcaat cacgccattc gccaagccta cggcgacagc attgcaagtg
    19441 gttactatcc ggcttttgtg atctttttgg atttagatcc gacaatggtt gatgtaaacg
    19501 ttcacccggc taaacacgag gtgcgctttc atcaaggaag actggtacac gactttattt
    19561 tgcaaggcgt actcaacagc ctgcaacaaa cccaacctac gcttgaactg cgaaatgaag
    19621 tgaacgagcc gttaccgagc tatgttcaag acactaaccg ccaagctgcg gggcaaaata
    19681 tttttgtgtc gcaaaccgct ccacaagcgg ccgaatttac gcaaaaaaat gcaagtcgat
    19741 caagctatac gcctaaattt gaacgaagca acagtgtgag ttcgtccgct caaaaatggt
    19801 ataaggaatt ggttggtggc gatttgcacc aaacaacgcc aactatcaaa acgcaaagca
    19861 cgcttgttga gccaaaaaca gaaccccaaa ctgaacataa aacagaagcc gtttcgcaaa
    19921 aaattacgca aattgcaccg cttgcaagcc accattcaca agccttagcg attgtaaaaa
    19981 accaagcggt gttaattaaa gaagaggaaa atttttacct gcttccactg gcaacattag
    20041 ccgagcttaa actcaagcta caactggaaa aagggcaatc tcaagcgttg ttgatcccac
    20101 tgaatctcag tttagatcaa caacaaaccg aacgctggca acaaaccaaa gaagaactaa
    20161 ctaagcttgg cttttcgatc agcgaaaaac agtggcaagc ccaaacacgg ctcacagtgc
    20221 tttccgtacc acaggctgtc agagaacaaa acctgcaaca gcttttattt gctctgttca
    20281 accaaacaca agcggtcaat ttagccgaat tttttgcaaa aaaatgcgaa aaacccaccg
    20341 cttgtacgct aagtgacgtg attgctctac tctcagaaat tgagctttat gccaatggga
    20401 aaaaagagct ggagggaata aggaaggaag tggatttttc caagttttta tagtctaaag
    20461 ggcaataccc tttagccaaa taaatgagag tggctgccaa ttcgcagcag ctctagctta
    20521 tcatcttcca cttttcgata gataagaacc aaatcaggtt ggatatggca atccctaaag
    20581 cctttccatt cgccggttaa agcgtgatcg cagtatttct caggtaatgg cttgtcatta
    20641 atcagaaaat acaacgcctc aattaatgaa gaggataaag actcaaccgc acatttttta
    20701 tagtcacgtt taaaagcatt actatactca atctctcgca ttgagttgct ccattaaatc
    20761 cgcaatacta tttgctgaat gtagcttttc ctcgccattc ttaacagccc ttaatacttc
    20821 tgctgtctca aaattaggct gataatcaaa ctgaaaacgt ttttctgttg cgattttcgc
    20881 taatgtcatt cgcatcacat cagagacagt caagccgata ccggctaaca cctcacttgc
    20941 ttgctcttta atttcagggc tgattcttac gcttactaat gcactatttg ccatcaaaaa
    21001 ctccttttgt atatcaaatg atatacaaaa taacatttaa caaaaaattg tgcaataaaa
    21061 aagaaattca aagaaaagaa atgaatagtt aaaattagct cgttacaaga aatcattgag
    21121 ttactcaaga aatcaaggta atataaagaa gtctgggcgg aacacgaacc cgcccctagc
    21181 atctagccgc ttacaagaac agatagatta gttgaaagag cgtgaaaatt tcaactgctg
    21241 cacacagaaa cccaagtgca actaaaatca atagtagttc tttcattgta agcctcctta
    21301 gtgctactaa ggatgattta ggctcaggct actgcgaata gccctaacga gcctaccagt
    21361 agcacaatca ggattgtaac aaaaaagctg tgaatttcac ggcttttttt gtatctatat
    21421 acagtatatt aatatgaata aaaaacccct tgcccttttt ttaatggggc cgactgcctc
    21481 cgggaaaacc gatttagcta ttgctttacg ccaagaattg ccggttgagg taatcagtgt
    21541 tgattctgcc ttaatttaca aaggaatgga tattggcacg gcaaagccaa gcaaggaaga
    21601 gcttgccctt gcaccgcatc gcttgattga tattttagac cctgccgaga gctattcggc
    21661 gatgaatttc cacgctgatg cgttgcgtga aatggcagat attacctcac agggtaaaat
    21721 tccgctcttg gtgggtggca caatgcttta ttacaaggca ttgcttgaag ggttatctcc
    21781 cctgccctct gccgatccag ccattcgggc tgaaatcgaa agccgagcgg cacaacacgg
    21841 ctgggcaaca ttgcatcaag aattagtgaa aatcgacccg attgccggtg caagaattaa
    21901 cccaaatgac agccaacgga tcaaccgagc gttagaagtt ttttacatta ccggcaaaac
    21961 gatgaccgaa ctcactgctc agcaaggtga ggctctacct tataatatct tacaatttgc
    22021 aatcgctcct caagatcgtg cagttctaca ccaacggatt gaacaacgct ttcacaaaat
    22081 gattgagcta ggctttgaag aggaggtcaa gcggttattt ttacgcaaag atttgcatat
    22141 taatttgccc tcgattcgct gtgtgggcta ccgccaaatg tgggaatatt tgcaaggcga
    22201 tatttcgctc gatgaggcga tctttaaagg catttgtgcc acccgccagc tggcaaaacg
    22261 gcagattact tggctgcgtg gctggcaagg cgaactgact tggttagata gtttagctcc
    22321 gaccagttct aaagcaaaaa tggtggaaaa gatcgatcat tatttaaaga attgtgataa
    22381 tatctaaacg cctttggcac ttatcgcatt atttggtgtc ttaaaaatca cacaactata
    22441 aaaaggaaaa taacaatggc aaaaggtcaa tctttacaag atccatattt gaatgcactt
    22501 cgtcgtgagc gtattcctgt ttcaatttat ttagttaacg gcattaaatt acaaggtcag
    22561 atcgagtcgt tcgaccaatt tgtgatttta ttaaaaaaca ctgtaagcca aatggtgtat
    22621 aaacacgcta tttcaacggt tgttcctgcc cgtgcgttat cgcataacaa taacaaccaa
    22681 gcccaacacg ctcagcctgc tgccgaagta actgaacaag cggaataata acattattac
    22741 tcaaccaatg ttaaacgaat cattgccaaa ctcagaagaa aattctgcgt ttggctttgc
    22801 tttatctgaa gcaagccaaa ctacagaagc cgcctcgcct accgatccag aaaatcaaga
    22861 taaagccatt ttggtgcatc tctatctcgg gcaacataaa gatgttgaaa atttgctgga
    22921 atttaaaacg ctggcagaat ctgccggtgt cgaagtgctg gcaacgctta ccaccagccg
    22981 ccaaagttcg catatcaaat attatgtggg gcaaggcaaa gcggagggaa ttgccgaagc
    23041 ggtagaacga ctcggggcaa cagtagtgct ggtgaaccat caacttagcc cggctcagac
    23101 ccgtaatttg caatcgttat gcggctgccg tgtggtggat agaaccggct tgattttgga
    23161 tattttcgct caacgggcaa gatcgcacga aggcaaacta caagtcgagc tggcacaact
    23221 tcgccattta tcgacccgtt tggttcgccg agttactaat caagaccaac aaaaaggcgg
    23281 agcagttggc ttgcgtggtc caggtgaaac acagctcgaa accgatcgcc gtttgattaa
    23341 agtgcgaatc caacagttac tcaaccgctt ggaaaaagtc agcaagcaac gcaatcaaaa
    23401 tcgaaaaacc agacaaaaag ccgatattcc aacagtatcc ttagtgggct ataccaatgc
    23461 cggcaaatct accctgttta atgcgattac ccaagctggt gtttatgctg ccgatcaact
    23521 ttttgcgacc cttgacccga ctttacgccg gattcaggtg caagatgtcg gcacgacaat
    23581 tcttgccgat acggtaggtt ttatccgttt tctaccgcac gatttagttt cagcctttaa
    23641 atctaccctg caagaaacca ccgaagctac tttactgctc cacgtcattg acggctcaga
    23701 cgaacgcaaa aacgaaaata ttgatgcggt aaatcaagtg ttggacgaaa tcggagcgtt
    23761 agatattcct actttattgg tattcaataa aattgataaa ttagaaggtg tagaaccgca
    23821 tattgaacgc aatgccgatg gcacgccagt tgccgtgtat ctttccgccc aagaaaatag
    23881 cggcattgag ctgttattcc aagccattca agaaaggctt cgcagtaact tagtgtgtga
    23941 aaacttgctc ctcccggtta ctgccggtgc aatttacgca caatttaaag cagaaaactg
    24001 cattaaaacc gagcatttta atgagttcgg tgaacggatt atctctattg aaattaacga
    24061 agtgcaatgg aacaaatggg tgaaacaatt tccggatcta gccgaatata ttgagctggc
    24121 aaaatggggg taattaatct cacttctgag taagttatat ttaaattatg caagcggtca
    24181 attctttacg tttttttgca aaaaagtgta aagaattgac cgcttgtttt atccattaaa
    24241 tccaatttag ccaataaaaa aatccctcaa ataaaccaga tttccccgtt ctatttgaag
    24301 gattttattt attcttcccc aaaagtgggg aagaaaagct taatcaagca gttactacca
    24361 ctgataacct acgcttgcag cagcacctgt atcaccacgg ctgttagagt taacactgaa
    24421 tttgataccc actttaccat tatctgataa acgagaataa cctacagcaa tagctccttg
    24481 accacggtaa gcacctacac cggctgaaac catcgattta ccaggttgtg ttacgtgata
    24541 taaattacct gcggctaatg cacctgcaat acctgcacgt aagtctttat ctacgttact
    24601 gatacgattg ttcagacgat tttctgcacc tttcaactga ttcacattaa cagcatcagt
    24661 gcctttcaca cccggagcaa cgttcgtaat acgtgtttgc gcattcttgc tacctgcaat
    24721 gttaagctca tttacgccat cttttgcaac agttgaactg atagttgtac caccaacttt
    24781 cactgaatca aattcaagat tgcttgaggt tgccacttca acagttgaac ctttacgtga
    24841 aacaacgatg tttttgccac ctgaaacggc cactttacca ttgttattaa cacgttgagc
    24901 acctttctct gaagtttcag ctacattacc atcaacagtg agataccagc ctgtattctg
    24961 cattgcatta tacaactggc taccgctcac aacatcttta cttgttgccg ttacatcacc
    25021 atctttaacg ttagtgatct tcgtgccttt cgcatcaata ccgcctttag ttacacttgg
    25081 gccgtcttta atggttaaac cattgttatt tacggttgtg tcgccagttg ttacagaacc
    25141 atcttccgtt aaatcaattg ctttcgctaa ttgtacctct aatgtgtctg taccattcgc
    25201 tttcacattg atgttgcctg atgctgactt aaagttttca gaagctgttt tatccacacc
    25261 ttcacctttc actttaactt cagtattcag tttctgacgg ttcttcacat cttcattgtt
    25321 accagagaag attaaaccat catttaaggt tgcaacttgt tctgtgacag ttttaccgtc
    25381 tttatctttg gtctcataaa cgatacgagt cttaccgttc tcacccggta atccatcttt
    25441 accatttgta ccatcaacac cttcagcacc atctttcact gcaatatttg cacttgcacc
    25501 atctttaccg tcagcgcctt tcggaccggt taagccgata gagccatctt taccgtttaa
    25561 tacaacagaa gcgccatctt tacctgtagc tccaattgaa ccatcaacac cgtctttacc
    25621 gtcacggcct gcagtaattg tgccgtcacc tttaatggtt acgccatctt taccgtcttt
    25681 gccagcaata ccgatttgac cgtctttacc atctttacca ccaatggtta agttgttttt
    25741 caagccgaag gtcacgttat taccttcttt agtaataata atgttaccgt ctttgttgtt
    25801 tagatcaact gttgctcctg gtttaacgtt gcctttgtct ttaccatttg ctgttaagtt
    25861 ccaacctgcg ttagcaactt cttgcacttg atataactgg ctaccattta ccgcatcttt
    25921 gctggttgca gaaatttcac cgtcttgaac gttaatgatt ttcttatcgc ctgcattgat
    25981 accggcttta gttacacttg ggccatcttt gatagttaaa ccatcattat ttaccgtagt
    26041 atcaccaata gttacagaac catctttcgt taaatcaatg tctttcgcta atttaactgt
    26101 taacgcacca ttgttgttta caacaccgat attgttatcg gacaggttcg ttaaatcagt
    26161 atcaccgcct gagattttta acgtctcatt aagtttctta acgatttctt taccgtcatc
    26221 acctacgaat cttaagccat cgttcagtgt tgcaacttcc tcagttgtac cgtctggttt
    26281 ctcataaacg atacgggttt tgctttcgcc atctttaccg tcgttaccgt caaggccttt
    26341 agcaccgtct tttacagaga tagttgcact tgcaccatct ttaccgtcag cgcctttcgg
    26401 accggttaag ccgatagagc catctttacc gtttaaagta acaccaactg taccatctgc
    26461 acctttaaca ccgattgaac catctttacc tggaccgcca atggtaaaat cgttatttaa
    26521 cgtaaagtca atcacattat cacttactgt tgtgatgatg tttttgtctt tagaggtgta
    26581 cttaacagtt tcacctagtt taacgtgatc agtttcacca ttatccgctg caatgctaaa
    26641 gcctttctcg ataatatcgt tgcttgcatt tacaacatca ttaatattcg cagcgttagt
    26701 accaacttct tttaattgat cgtctgttaa gtcgttcact gaagtgattg gcttaccatc
    26761 tttaccgtta atgccgtctt tacctgctaa accgtcaata ccgctcttca cgttaacaat
    26821 ttggttgccg ccattgtcta gaccgtcttc agtaagctta actgctttac catctgtgcc
    26881 atcttttggc gtgatagtta cgccattatt atttactact gttgttggac ctttaccttc
    26941 ttgaccattc tctccatctt gtggtttgaa tgtagctgag ttcaagtctt taagatcttt
    27001 agctaattga acagataatt taccatcttt gctgtttacg ccgatgttat ttgcagttag
    27061 cttatcttta tcagctccac caatgatttc taaggtttca ttcaatttct tagatactag
    27121 tgttgcatca tcacctttaa agtgtaaacc atcatttaaa gtagctactt gctctggttc
    27181 acctatagga tcaccattgc catctgttgg ctgatattgg atacgagtta caccatcttt
    27241 acctggtaaa ccatctttac caacaagccc tggtttgccc tcagtacctt taatggttaa
    27301 accatcttta ccatttgtac cgttcaagcc gattgaacca tctttaccgt taagaacaac
    27361 tgcagaaccg tctttaccgt ttacaccgat agtaccatca ataccatctt tgccagggcg
    27421 acctacagtg atagctccat ctttaccatc taaagcaata gcatctttac cgtctttacc
    27481 agcaatacca actttacctg gttcaccatc tttaccaaca gtaatattat tcactaggct
    27541 aacttctaat gcatggccac catctttctc aacaacgttt gttttaacat tgccatcacc
    27601 aatgatctta gctgtcgtac ctaagttttg cttaaactcc ttgttgttct catcttttag
    27661 accaaacgca tctgttgtca cctcagtaac agtctcagta acagtatttt tcaagtcacc
    27721 gacgttaacc gcattgttaa gaacatcacc tgctgcctca gtaagtttaa caggttgacc
    27781 atctttacct tttaagccgc tatcaacatt ggtgatttgg ttaccaccgt tgcttaagcc
    27841 atcctctgtt aaggtaacat tagatttagt tgcatcatta ggatctttct tagcaatcgt
    27901 tacaccatct ttattgataa ctgtagaatc accttgtgtg ccgaatgttg cactatctaa
    27961 gcctgctaga tcttttgcta acgccacagt aatggtattg ccaacaacct gtgtcatgat
    28021 atttttacca gcatcaaact tagcccaatc agtgtttgtt gtagcacctt taatatccac
    28081 ctgcgtattt aatttcgcat cgtgaacttt gccatcattc gcaccgaatt ttaagccatc
    28141 gttaagtgtt gcaacttctt ctttatcacc atttggtttt tcgtaaacaa tacgagtttt
    28201 gctttcgccg tctttaccgt cgttgccgtc aagaccttta gcacctttct caactttaat
    28261 tgatgcttta ccatcttttc catcttttcc atttacgcca tcaacgccgt ggaatacgat
    28321 ggcttctggt ttaattgtta caccatcttt accatcagca cctttaacac caactgaacc
    28381 atctttacct ggtttaccac caacagagat gttatcggat aagccaaaat cgatggtatt
    28441 atttgttacg gttgtaacaa cattcttatc ggttgaggtg tacttaacag tttcacctaa
    28501 cttcacattg tcttctttag caccacctcc taagccagag tgatccgctg taatgttaaa
    28561 gccagtttta actaaagtgt cagctactgc gtaaagttgt gaaccgttaa tcgcatctgt
    28621 tgaattggaa gcaattttac ccggtgcaac aaacttaatt tggcgagcat tggttgcatt
    28681 acccacactc acataatcac catcaacaac attttcatcg ccagcaaagc tgctatattt
    28741 taatgtgcca acagttgcag tatcttcttt agttgcggtt gcttcagtag agtcagaacc
    28801 tagaattaca gaaccctttg tcttaacagt aacattagaa cctaatacga aagtatcttt
    28861 acctgaaact gtattgttat aacctaaagc cgcagcacga atacccgaaa cagtactatt
    28921 tacaccggct gcaattgagt ttttaccctt tgcggttgag ttgtaagcaa tagcagttgc
    28981 ataattacct tttgatttgg cctcaaaacc aattgctgtg ccaccttcat gcgtaatatt
    29041 ggcacgtgta cctactgctg tcgcattcgc cttacttaca ttagcactat aacctatcgc
    29101 aacagaatta ttaccacttg aattagccgt atcaccaatt gccattgcac catctttgct
    29161 cgcgttagaa tgcgaaccaa tactgatcac tttttctgca tgagagtttg cattccaacc
    29221 aatgctaata gcattacttt tccaggcttt agaagcatta ccaaaagcaa tcgcaccgat
    29281 tcctgttgta ttcgaattta caccaaaggc gatagaacca tcacctgttg cacttgcatt
    29341 tgttgaaaac gccaaagtat cattcgaatt tgcacgagta ttcgtaccca aagcaaatgc
    29401 attatttgct actttcgctg tatcaggatt aattacctgt cctaattcag aaatatttga
    29461 attctctaat ttaaagccac cggaccaagc gttagtacca atggcaaatg aatggttacc
    29521 attagttttc gctttagtac cgattgagaa tgcatctgtt ccaaatgcta aagtattatt
    29581 ttgcccatca taaattgaac gtctattttc atgaccgtaa cttccatcca ctgtctgtgg
    29641 agtaccacta ccaatcgcaa aagaacggtc acctagcgct gtagccgaag caccaatggc
    29701 gaagctagct gtagcatttg cccaagatgt tgaaccaatt gcagtagaat aatctgcaat
    29761 tgcagcagat tgacgcattg cagcaaatga gttaaatcct tccgcacgtg cagaggtacc
    29821 taatgctaca gaaccaatac ttgcagtacg agaaaaagaa ccaattgcaa tagacaatcc
    29881 ccctccagaa tatgaataat caccaatggc tatgcctgac gctttcggtt ttcctctact
    29941 ttttctgtta ttgtctaaag ctacagcatt gtgtccaatt acaattgctg aggtagtatt
    30001 agacttatac aataatctag aatccttctc cttattaaca gagttattag attcttgaag
    30061 cttagttctg tctgcatagg ataagctacc tgggttaaag tagttatatg gattcgtaat
    30121 agaatctgta gccaaactag tgtttgtata aatatctata ccaccattaa caactttttt
    30181 tggtgctttt tgttgtactt ctattctatc aataatcacc gccgcttctg ccggtgtagc
    30241 agaaagcata aaggctgcca ttccactgat aagggttgct ccccctagaa tgctagccgc
    30301 tgctaattta atagtaccgc gcttatcagt agaagaagcg gctttacctt gcgatcgagc
    30361 aagttcagac acaacttcca ttttttgcgt cgtttggtta aaaataacct taaatatttt
    30421 gttcataatt ttataccttt agaattatta taaagcttac ttcgtaaata attcggcaat
    30481 t
//
LOCUS       DANLXS010000009        28656 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.009, whole genome shotgun sequence.
ACCESSION   DANLXS010000009 DANLXS010000000
VERSION     DANLXS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28656
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.009"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            25..999
                     /gene="znuA"
                     /locus_tag="PXD71_000326"
     CDS             25..999
                     /gene="znuA"
                     /locus_tag="PXD71_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDL6290492.1"
                     /translation="MFKRTLIGLAVLGASVAQADVLTSIKPLGFIASAITEGVTETKV
                     LLPVTASPHDYSLKPSDVTQLQSAQLVVWVGDGLEGFLEKSIEKLPREKVLTLEDVDA
                     IDDIVDKASKKEDKDDDHDHKHEHKHEHKHEHKHEGHNHEGHNHDHHGHSHDEDWHIW
                     LSSEASELAAEQIAARLSEQMPEHKAKIAENLAKFKANLTAKKAEIAKQLEPVKAKGY
                     YTFHDAYGYFEREYGLNSLGSFTINPTVAPGAKTLTAIKKNIAKNKAQCLFAEPQFTP
                     KVIETLAKETKVNVGQLDPLGAKVQLSATAYPEFLQVLADEFSQCLTK"
     gene            complement(1051..1371)
                     /locus_tag="PXD71_000327"
     CDS             complement(1051..1371)
                     /locus_tag="PXD71_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HDL6290493.1"
                     /translation="MIYPTCPECKSENTYHDSIQFVCPDCAYEWTGEETDQDDDVLIV
                     KDSNGNLLSDGDDVLLIKDLKLKGSSEVLKKGTKFKGIRLAQGDHNVDCGKIMLKSEF
                     LKKA"
     gene            1512..3875
                     /gene="rnr"
                     /locus_tag="PXD71_000328"
     CDS             1512..3875
                     /gene="rnr"
                     /locus_tag="PXD71_000328"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL6290494.1"
                     /translation="MIVDPNYQQELEKYENPVPSREFILNTIREHNAPMSRDELLSAF
                     HIDDEERMEGIRRRLRAMENDGELVFTKGKRYALPEKMDLIKGTVIGHRDGYGFLQVE
                     GHEKGATKSEDWFIPNAQMVKVMHGDFVLAQPNGTDRRGRKEVRIVRVLEARKKQIVG
                     RFFLESGIGFVVPDDSRVNQDILIPDEHRMGARMGQVVVVELQERKASFSRPVGIITE
                     ILGDNLAPGMEIEIALRNHDIPHIWPEGVEKQIRQFSSEEVPEEAKQGRVDLRDLPLI
                     TIDGEDARDFDDAVFAKKEGNGWRLWVAIADVSYYVRPKTALDLEALNRGNSVYFPNR
                     VVPMLPEVLSNGLCSLNPQVDRLCLVAEMSVSSKGELQDYQFYEAVMNSHARLTYTKV
                     WKMLEGDEALRERYAPLVPHIEELYAMFQTLMKARHKRGAIEFETIENQFIFNPQGRI
                     ERIEPLIRNDAHKLIEECMILANIAAARFVEKANEPALYRIHDKPSEEKLLSFKSFVR
                     ECGLTWDVGLDPTPKDYGVLLEQIAERADKELIQTMLLRSLKQAVYAADNIGHFGLAL
                     TEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRYYTDGGGYHYKLDDMDEFGDKCSA
                     TERRADEATREVADWLKCEFMQDHLGEEFDGVISSVTGFGLFVKLNELLIDGLVHIST
                     LDNDYYHYDADRQRLIGGSGIIYRLGDAVRVKVINVNLDEKKIDFELLTGNKKTGKTA
                     KKKAKATEVPVFKEPKTVKKAKKVAEKKPAKSAKKRTKTTASKSRKK"
     gene            3879..4145
                     /locus_tag="PXD71_000329"
     CDS             3879..4145
                     /locus_tag="PXD71_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290495.1"
                     /translation="MDYKKQILFSLLFALVIAIISYFLFTYIPFDCSDLSKLNDSQRL
                     ECRTAEFLGVYYWLPHTTTLFFGSVLLYWAVYGMISLVQAVLKR"
     gene            4368..4826
                     /gene="mraZ"
                     /locus_tag="PXD71_000330"
     CDS             4368..4826
                     /gene="mraZ"
                     /locus_tag="PXD71_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL6290496.1"
                     /translation="MFRGASSISIDSKGRIAIPTRYRAELLEKHHGILVCTVDIRQPC
                     LLLYPLHEWEMVEQKLLALSNFDPVQRRIQRVMQGFATECEMDSAGRILLSPTLRQHA
                     QLEQQIMLVGQLNKFEIWQDKQWQAQIAEDLAFGSSAEILDCEALKNLSL"
     gene            4842..5780
                     /gene="rsmH"
                     /locus_tag="PXD71_000331"
     CDS             4842..5780
                     /gene="rsmH"
                     /locus_tag="PXD71_000331"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL6290497.1"
                     /translation="MSENPKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSK
                     LGENGRLIATDRDPRAIAEAQTISDPRFQIEHTAFSAIPELCERMGLVGKIDGILLDL
                     GVSSPQLDEAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSAEDLAWVLKEFGEERF
                     AKRIAQAVVSYNKSANEKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINGEL
                     DELEKALNSALTVLAPEGRLSIISFHSLEDRMVKQFVRKHSKGMDVPRGLPILEAELT
                     KNIPLKTIGKAIMPSEAEIEANARSRSAVLRIAEKR"
     gene            5799..6110
                     /gene="ftsL"
                     /locus_tag="PXD71_000332"
     CDS             5799..6110
                     /gene="ftsL"
                     /locus_tag="PXD71_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL6290498.1"
                     /translation="MSNERYPLHQIIIDDIFSHNKVALLLLIGVVISAVATIWITHQT
                     RLSVSEQGQLIQANQKLESQYTNLQLEENSRSSRARVDAAAKSFGLQPIKKEQEIILV
                     E"
     gene            6114..8174
                     /locus_tag="PXD71_000333"
     CDS             6114..8174
                     /locus_tag="PXD71_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL6290499.1"
                     /translation="MVKSVKLKRKQEAPVAKAEPQQAEGKKTPSFLPTRFRVVVFFML
                     LMVGGLLAQTAYIQLFDSERLIKEANNRSLRTKELQFTRGRILDRNGRFLSISVPMYS
                     LTLDPREYFDTKLRRSNESWRALAIEMETSKGKIEANVNKFIEKKNFSKEKVDFDPRS
                     ILNTKSEGYWTQLAQVTGLDYNSLIEKVRNNPSSVFLRLDNENLVRERQKFQALSKEI
                     GRSYNDIMDELYSKNRQRFLYLSRHQSEAISEYAKELDIDALVIKVESRRFYPLAEES
                     SQLIGFTDKDDMHGSEGLERSFDSLLIGKNGKQIIRKDAKGNIVENIRSEKQYDPQDV
                     MLSIDEELQSMVYGEIKKAVQENNAESGTAVLVDVQTGEILAMANAPSFNPNKRDSFK
                     PELMRNRAITDTFEPGSTVKPFTVLTALQNGVTYRDEVIGTRPFVVNGHTIKDVAPRD
                     SLTLTGILQKSSNIGVSKLALRMPANALVDTYTKVGFGKDTGLGLGEQRGTNGDRKRW
                     ADIERATLSYGYGLNVTPLQLARAYATLGSFGLYRPLSITKVDPPVIGERVLPEKTTR
                     DVVHMMESVAAKGEGGHRAAVDGYRVAIKTGTIHKLEKGKYVEKYIAYTAGLAPASDP
                     RFALVVLINEPKAGNYYGGFVSAPLFSRIMGYTLKQRNIKPDNLTENTQSAVREIKLE
                     RPIN"
     gene            8183..9673
                     /gene="murE"
                     /locus_tag="PXD71_000334"
     CDS             8183..9673
                     /gene="murE"
                     /locus_tag="PXD71_000334"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDL6290500.1"
                     /translation="MKRLLPFLTELDAWVEELTELKQMTLDSRQVQAGDLFVALKGHQ
                     VDGRKFIPNAIEQGAAIILAEADEDQPEIELDPKFAKFNLDRTACCKVVSVPRLPKLL
                     SEIAGALYHNPSHKLTLAGITGTNGKTTTAQLLAQWHNLLGGKSAVMGTIGNGLYGQV
                     QEAVNTTGSAIEIQHNLANFVELGADFCAMEVSSHGLAQFRAEALDFDLAIFTNLSRD
                     HLDYHNTMEEYAQAKFRLFNELSTKAQVINADDEIGREWLTQLPNAVAVSTDPKFAGN
                     HQFVKATAVKFTLQGASIAFESSWGNGELHSRLIGAFNVNNLLTAFAGLLALGFDIQE
                     LVKTAPNLVGVAGRMECITAPNKPMVIVDYAHTPDALEKALQAARLHCEGELWCIFGC
                     GGDRDAGKRPLMATIAEKLTDKVIATDDNPRTEDNQKIMADIVKGFSKPQQIIHNREE
                     AIKTAIEQAKATDVILIAGKGHEDYQIIGTEKLHFSDQETARKYLF"
     gene            9761..11155
                     /gene="murF"
                     /locus_tag="PXD71_000335"
     CDS             9761..11155
                     /gene="murF"
                     /locus_tag="PXD71_000335"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL6290501.1"
                     /translation="MIKLTTQQIAEILNAKLIGNGETVVETTSTDTRQAVENGLFFAL
                     KGENFDAHHYLQNAVAQGCVAVVVERECEIDVPQLVVADTRLALGELAKWLKAKLNPK
                     TVAMTGSSGKTTVKEMTAKILQKMTACEQEVLYTFGNLNNDLGVPMTLLRLTEQHKYA
                     VIELGANHIGEIAYTTAIAQPDACLVNNVAAAHLEGFGSLAGVAQAKGEIYRGLKENG
                     KAIVNLAFYYPQWQKEIGSREMQSFSYIGSDSDSYADYWAEDVELHLSGSRFTLHTPQ
                     GEIAIDLPYLGKHNVSNALAATSLAMSVGATLEAVKAGLEQRSMVKGRLYPVQINDHC
                     LFIDDTYNANVDSMKSAISVLENYPAYRIFAVGDMAELGEESSKCHQEVADFAKNAAL
                     DLIVSFGKESAVISQQAEHHFTDKQQMVDFLLPIILQKIEQKQPLVLLAKGSRSQKME
                     TVISELCKPFNLNF"
     gene            11176..12258
                     /gene="mraY"
                     /locus_tag="PXD71_000336"
     CDS             11176..12258
                     /gene="mraY"
                     /locus_tag="PXD71_000336"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL6290502.1"
                     /translation="MLVWLAELLVQYNTAFNVVSYITFRAIMALLTAMGIGLWIGPKV
                     IRRLQILKFGQEVRNDGPESHFKKRGTPTMGGVMILAAIGVSTLLWADLRNSYVWFTL
                     FVLFGYGAVGFVDDYRKISRKNTDGLIARWKYFWLSAIALVAVFGMYAVGKDTAATQL
                     VVPFFKDVMPQLGLFYIVLSYFVIVGTSNAVNLTDGLDGLAIVPTIMVASAFALIAWA
                     TGNFNFAQYLHIPFIPNAGELVILCTAIVGAGLGFLWYNTYPAQVFMGDVGSLSLGGA
                     LGVIAVLVRQELLLVVMGGVFVVEALSVILQVGSYKLRQKRIFRMAPIHHHFELKGWS
                     EPRVIVRFWIITLMLVLVGLVTLKLR"
     gene            12322..13623
                     /gene="murD"
                     /locus_tag="PXD71_000337"
     CDS             12322..13623
                     /gene="murD"
                     /locus_tag="PXD71_000337"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL6290503.1"
                     /translation="MQKQYENKTVTIIGLGTTGLSCVDFFKKKNANIQVIDTRANPAG
                     ADKLDVTIPLHTGGLNSEWLLKSDLIVISPGLAVATPEIQQAIQAGVEIVGDVELFCR
                     EAKAPIIAITGSNGKSTVTTLTTEMAKQAGIKVGMGGNIGVPVLSLLEGDYDLFVLEL
                     SSFQLETTYSLKAKAATVLNISEDHMDRYDSIADYRQAKLRIYDNAENIIVNGEDAQT
                     YPIQAVKKVIRFAEQNAEYSLRNQMLYSGDDAIIQTKQMLISGRHNEMNALAAMALAE
                     AANVPREGIVKALQIYGGLPHRFQAVATNDGVRWVNDSKATNVGSTVAALNGLNIAGT
                     LYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAALAELSEQSVLVETMQQAIEAI
                     RPQLKKDDMVLLSPACASLDQFKNFEQRGEVFMALAQGQHA"
     gene            13623..14801
                     /gene="ftsW"
                     /locus_tag="PXD71_000338"
     CDS             13623..14801
                     /gene="ftsW"
                     /locus_tag="PXD71_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HDL6290504.1"
                     /translation="MLERIKSEWDKWVRLTPSNSLYDRTLIWLFLGLLIIGFVMVTSA
                     SLPVSTRLNNDPFYFAIRDGLYIIASIIFCYVFVQIPIEKWEKHNLALFFISIGFLIA
                     VLIFGRSINGAVRWIPLGILNFQPAELAKLAVICYFASFYVRKYDEIRKEKASFWRPA
                     VILFLFGFLLILQPDLGSTFVLFVLTFSMLFIVGAKIMQFMFLGVVGTVLFAVLILTS
                     EYRLKRVTSFMDPFADAYGDGFQLSNSQMAFGQGEFWGQGLGNSVQKLEYLPEAHTDF
                     VMAVIGEEFGFFGIACIVLLLILLTVRALKISKESLVLEERFKGYMAFGIAIWVFLQG
                     FVNLGVASGLLPTKGLTFPLVSYGGSSLVIMSIAIAVLLRIDHENRAERIGHAKIKTI
                     "
     gene            14920..15975
                     /gene="murG"
                     /locus_tag="PXD71_000339"
     CDS             14920..15975
                     /gene="murG"
                     /locus_tag="PXD71_000339"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL6290505.1"
                     /translation="MAKKLLVMAGGTGGHVFPAIAVARELQKQGWQIRWLGTKDRMEA
                     TLVPKHGIEIDFIEISGLRGKGVAALLKAPFAIFKAVMQARKIIKNYQPDAVLGMGGY
                     VSGPGGIAAKLCGVPVILHEQNAVAGLTNVWLSKIARKVLQAFPTAFKEAEVVGNPVR
                     EDLSALPLPNERFAKRGYPINILVMGGSQGARVINQTVPEVAKVLGNNVFISHQVGKG
                     NLAGIEEVYQQTGNGIASEFIDDMKAAYEWADLVICRSGALTVCEIAAVGLPAVFVPF
                     QHKDRQQFLNANYLAADGAAVIIEQSDFTPKALQQALEPLIANRQLLLDMSMRARAKS
                     TPMAAKRVAEVIIQEAN"
     gene            16051..17478
                     /gene="murC"
                     /locus_tag="PXD71_000340"
     CDS             16051..17478
                     /gene="murC"
                     /locus_tag="PXD71_000340"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL6290506.1"
                     /translation="MKNFQDKVKKLVPEMRRVSQIHFIGIGGAGMSGIAEVLLNEGYQ
                     ISGSDIADRAVTQRLSAAGAKVFIGHQAENIAGASVVVVSTAIDETNPEIIAAREARI
                     PVIRRAEMLAEIMRFRHGIAIAGTHGKTTTTAMISMIYTEAKLDPTFVNGGLVKSTGK
                     NAHLGASRYLIAEADESDASFLHLQPMVSVVTNIEPDHMDTYGGDFEKMKETYVRFLR
                     NLPFYGLAVMCADDETVMEIAPRVGRQVITYGFSENADYRIEDYQQTGFQEHYTVVCP
                     SGERIDVLLNVPGMHNALNATAALAVAKEEGIGNEAILAALADFQGAGRRFDQLGSFI
                     RPNGKIMLVDDYGHHPTEVDVTIQAARSGWEGKRVVMIFQPHRYSRTRDLFDEFVQVL
                     SKVDALILLDVYAAGEAPIVGADSKALARSIRNLGKVDPILVSDTDQLGEVLDQIIQD
                     GDLILAQGAGSVSRISRGLAESWKN"
     gene            17568..18479
                     /locus_tag="PXD71_000341"
     CDS             17568..18479
                     /locus_tag="PXD71_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL6290507.1"
                     /translation="MSIKNEKIAVLFGGVSQEREVSLNSGAAVTAALKSLGYNVEGID
                     TKEFPIEKLKEKGIQRVFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFR
                     TKLLWNAVGLPTADMVVVQRGQAVDFDQIIAKLGLPVFVKSSCEGSSVGVFKVKTKEE
                     LAPAIHEALKFDSIVLVEEFLAGNEYSVPVLDGQVLPAVQVIPDGEFYDYHAKYVSDN
                     TQYVVPALNDERQAEVAKLVKAAYDVVGCRGWSRIDVMEDGEGKFRLVEVNTNPGMTS
                     HSIFPKSAATVGISFEKLVERVLELSA"
     gene            18479..19270
                     /locus_tag="PXD71_000342"
     CDS             18479..19270
                     /locus_tag="PXD71_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL6290508.1"
                     /translation="MGIFRKKNSTNVRLNVLNPKTPVSPRKWLVFIKPLIVLLCTLFV
                     FGVYSNWQNILESWDKTPIRAYALTHKTQFTTNADIRETLSKEPALKGYFGQNIQEVK
                     DKLLSIPWVKDVAVRKLYPDRLSITLLEHKPVAIWNDTNFVSEQGTVFSLPKDRIDKN
                     GLPLLYGPDTEGKSVLEAWNKIRADLNARNLDLTSVSVDNRGSWTIRLSNQVELRLGR
                     GEWTSKIDRFVIIFPEIHIPEGNRLAYVDLRYPHGAAVGFSPIKN"
     gene            19293..20564
                     /gene="ftsA"
                     /locus_tag="PXD71_000343"
     CDS             19293..20564
                     /gene="ftsA"
                     /locus_tag="PXD71_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL6290509.1"
                     /translation="MTKAVESKIIVGLDIGTSKVVAVVGEVLPDGVINVMGAGVCPSK
                     GVDRGGVIDLDAAVNSIQRAIEQAESIADCQIMGVTLAISGQHIVGLNEAGSSTLSGG
                     IVTQDNIESAVDMAKAIKLQDGLETLHMIPQEYHVDRLPATKNPIGLSGMRLQVQAHL
                     IACHQAWLKNLKNAVEHAKLKVDQVVFSGLASSYSVLTEDEKELGVCLIDIGGGTMDV
                     LVYTDGALRYSKVIPFAGNNITDYLARVFTTSRPEAESLKVGYGSAISPPTHNSDKKI
                     EVAGLGGRMARTFTRAQVATVTSQCYNDLLKVVEEELTQLRHELFQKGIKQELIAGVV
                     ITGGGSQIEDIVECAKSIFGSQVRVGYPLNITGLTDYVNKPQYATALGLLQYSHYNYT
                     EQGTSTDSNEDIFAPIGRGTKKIIDFFKSKF"
     gene            20597..21844
                     /gene="ftsZ"
                     /locus_tag="PXD71_000344"
     CDS             20597..21844
                     /gene="ftsZ"
                     /locus_tag="PXD71_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL6290510.1"
                     /translation="MFEPIIEQTQDAIIKVVGVGGGGGNAVDRMSRSADDIKGVEFFD
                     VNTDAQVLRKRTTRQTIQIGASTTKGLGAGADPMVGKQAAEEDREAIANALKGANMTF
                     IAVGMGGGTGTGAAPVVAQIAKEQGSLTVGVVTKPFRFEGPRRMRFADQGIKELSQYV
                     DSLIIIPNDKLRGLGKQTTAVDAFAAANDVLSNCVLGITNMITSSGGSTGADINVDFA
                     DVRTVMSGKGHAMIGTGFAEGEVGEGRAEKAMNDAISSPLLENVDISGASGMLINISA
                     GTDFLLEEVYAMMDLIYGFATEDAAIVFGCNYYPEMDGKVSVTLVATGIGQPEEALHM
                     PHKAQPVYAQQGNHVQATQPTQPNHAVQQPNFTQPSSYGQPNQFSGQPQPLQQPVAPK
                     PQSVDTAQIFNPNSIPGFMRNSQ"
     gene            21876..22796
                     /gene="lpxC"
                     /locus_tag="PXD71_000345"
     CDS             21876..22796
                     /gene="lpxC"
                     /locus_tag="PXD71_000345"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDL6290511.1"
                     /translation="MTKQRTLKQAAKVTGIGLHSGKKVTLTLRPAPANTGVIYARTDL
                     EPVVYFPASADSIRDTQLCTCMVNDDGVRISTVEHLNAAMSALGLDNVIVEVDAPEIP
                     IMDGSSSPFIYLLLDAGIEEQDAPKKFIRIKETVRVEEGDKWAELKPYNKGFKLNFTI
                     DFEHPLIPKNVGSYSIDFSAENFIQQLSRARTFTFMKDVEYLQSIGLALGGSLDNAIV
                     LDDYRILNEDGLRFKDELVRHKMLDSIGDLYMCGYNILGEFSAYKSGHGVNNKLLRAV
                     LENKNAWEFVTFEDKAEVPQGYQVQQEIFI"
     gene            22969..24651
                     /gene="recN"
                     /locus_tag="PXD71_000346"
     CDS             22969..24651
                     /gene="recN"
                     /locus_tag="PXD71_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL6290512.1"
                     /translation="MLTQLTINNFAIVRHLILELNEGMSVITGETGAGKSIAIDALGL
                     CLGYRSESGMIRNGSDKADISATFTMQPHSPAYLWLQEHELLDEDNPQECILRRMINI
                     DGRSKAFVNNRSLPVSQLRELGQYLIHLNGQHAPQLLLKNEYQLEIVDHYATLQPLLM
                     KMAKQYGNWKELNKQVKNFQKQCQENEARKQLLEYQVEELDEFAIKANEFEEMEEKYN
                     ILSSSEELTELSDEITRLLSDDEINANSLIYKALRNAENLAEVDSQYQGILDMLNEAL
                     IQIQEASSEVAQLASKIEQDPELLQELDNRIAQTISLARKHHIQPNELWQYHATLKAE
                     LQELLDFEDNEEQLIQNEKQAYQECITLAEEIYQKRLDATHKLSEQVTKQIKHLAMEN
                     GEFFIHIEHNLEKMSANGADYVEFNLRSNLGQQPQPLAKIASGGELSRISLAVQVLTA
                     NKLSTPTIIFDEVDVGISGSAATTVGKLLRKLGEKCQVLCVTHLPQVASYGNQQFNVQ
                     KYVENNQTETQMTLLTEAERIHALARLLGGSEITETVLANAKEMLNLAKNNE"
     gene            complement(24698..24773)
                     /locus_tag="PXD71_000347"
     tRNA            complement(24698..24773)
                     /locus_tag="PXD71_000347"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24738..24740),aa:Ala,seq:ggc)
     gene            complement(24853..26292)
                     /gene="gltX"
                     /locus_tag="PXD71_000348"
     CDS             complement(24853..26292)
                     /gene="gltX"
                     /locus_tag="PXD71_000348"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL6290513.1"
                     /translation="MKIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKH
                     NDGEFVLRIEDTDLERSTPEATAAILEGMEWLDLAWEHGPYFQTKRFDRYNQVIDQML
                     EAGTAYRCYCSKERLDEVRAEQEANKEKAKYDQHCLHNPQNDPSQPHVVRFKNPTEGS
                     VVFDDAVRGRIEIANSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNT
                     PRQINILKALGAPIPTYAHVSMINGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLV
                     RLGWGHGDQEIFSRDEMIELFDIHSVSKSASAFNTEKLQWLNQHYIRSLAPSYVAKHL
                     EWHMQTQGIDFTSGPKLEEIIPVLGERCKTLKELAESSRYFYQEFKAYDEKAAAKNFK
                     AEAVAPLAKLLEKLTACNDWTVENIHEAMNQTAAELEIGMGKVGMPFRLAVTGSGQSP
                     SMDITAKLVGKERTLARLQKAIEFIQANS"
     gene            26467..26542
                     /locus_tag="PXD71_000349"
     tRNA            26467..26542
                     /locus_tag="PXD71_000349"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26500..26502,aa:Val,seq:tac)
     gene            26591..26666
                     /locus_tag="PXD71_000350"
     tRNA            26591..26666
                     /locus_tag="PXD71_000350"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26624..26626,aa:Val,seq:tac)
     gene            26719..26794
                     /locus_tag="PXD71_000351"
     tRNA            26719..26794
                     /locus_tag="PXD71_000351"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26752..26754,aa:Val,seq:tac)
     gene            26843..26918
                     /locus_tag="PXD71_000352"
     tRNA            26843..26918
                     /locus_tag="PXD71_000352"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26876..26878,aa:Val,seq:tac)
     gene            complement(27072..28565)
                     /gene="mltF"
                     /locus_tag="PXD71_000353"
     CDS             complement(27072..28565)
                     /gene="mltF"
                     /locus_tag="PXD71_000353"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDL6290514.1"
                     /translation="MKGLIARLFIAVALLLWAWDMIFPWQKIIHSEANHYTQIQQSKT
                     LKVGMINHPLSYFVGPEGKSGIEYDLATAFAQYLKVNLDIKTYDSSEKLFEALRNNDV
                     DIAAAGLLYHSELNDEFQIGPSYYSASWQLVYKKGSARPYRLSDLRENVIIPAGSAVI
                     PILNQLKVDNPELKWQISDKFTQEELLLKVAQGEISHTIAMSVDISAAQHINPNLAVG
                     FDLTDENPVLWYLANSSYSELQSVLLDFMSYSMETGLISRIEEKYFNHLAKFDYVDIQ
                     SYLNAIKTILPKYEPLFRAHKGELEWQMLAAIAYQESHWDPNATSPTGVRGIMMLTKD
                     TADRMKISDRTNPAQSIKAGSEYLHMLMRQMPETIPQADRIWYSLAAYNMGLGHLLDL
                     RRLTKQLGGDPDNWLDVKKNLPLLAEKRYYANLKYGYARGYEALQYVENIRRYYGSII
                     NYQRVEEQKMQEQENLPDGQIKQENSSYEKIEEKEIPTDKEITPASN"
BASE COUNT         8697 a         5509 c         6388 g         8062 t
ORIGIN      
        1 attaaatgcc ataaggggaa ttttatgttt aaaagaacat taatcggtct agccgtgtta
       61 ggtgcaagtg ttgctcaagc ggatgtatta accagtatta agcctttagg ctttattgcc
      121 agtgccatta ccgaaggggt aactgaaact aaagtattat taccggtaac tgcctctcca
      181 cacgattaca gcttaaaacc ttcagatgtg actcaattac aatctgctca attagtggtt
      241 tgggtgggtg acggcttgga aggcttttta gaaaaaagta ttgaaaaact gccgagagaa
      301 aaagtattaa cccttgaaga tgttgatgcg attgatgata ttgtcgataa agcaagcaaa
      361 aaagaagata aagacgatga tcacgatcat aaacatgaac ataagcacga acacaagcac
      421 gagcataaac acgaagggca taatcacgaa ggacataacc acgatcatca cggacatagc
      481 cacgatgaag attggcatat ttggctctca agcgaggcaa gtgagctggc agcagagcaa
      541 attgcggcac gtttaagcga acaaatgcct gaacacaaag cgaaaattgc ggaaaatctt
      601 gcaaaattta aagcaaatct aaccgctaaa aaagccgaaa ttgcgaaaca attagagccg
      661 gtaaaagcca aaggttacta cacattccac gatgcttacg gctattttga aagagaatat
      721 ggtttaaatt cattgggttc atttaccatt aacccaactg ttgccccggg tgcaaaaaca
      781 cttacagcaa tcaagaaaaa cattgcgaaa aacaaagcac aatgtttatt tgccgagccg
      841 caatttacgc cgaaagtaat tgaaacctta gcaaaagaaa ctaaagtaaa tgttggccaa
      901 ttagacccac tcggtgcaaa agttcagtta agtgcgactg cttaccctga atttttacaa
      961 gttttggctg acgaatttag ccaatgttta acaaaataag ttaaaaatcc cccatttaaa
     1021 tataaatggg ggattttgcg attacatcac ttaagctttt tttaagaatt ctgatttcaa
     1081 cataatttta ccgcaatcga cattatggtc accttgagct aaacggattc ctttgaattt
     1141 tgtacctttt ttcagtactt cagacgagcc ttttaatttt aaatctttga ttaataatac
     1201 atcgtccccg tcagataata aattgccatt actgtctttt acaattagca catcatcatc
     1261 ttgatccgtt tcttcgcccg tccattcata cgcacaatcc gggcaaacaa attgaattga
     1321 gtcgtggtaa gtattttcgc ttttacattc agggcaggtt gggtaaatca ttttaattcc
     1381 tcaaattaat aaagaaccag attatatacc gaaatccagc aaaacgctaa gcggtcatat
     1441 tttgccgatt ttttaccaat cgagctaaaa gtcagttaga atagccttaa ttacacaaaa
     1501 aggaggatac tatgatagtc gatccgaatt atcagcaaga attagaaaaa tacgaaaatc
     1561 cggtaccaag ccgagagttt attttaaata caattcgtga acataacgcc ccaatgagcc
     1621 gtgatgaatt attgtcggct tttcatattg acgatgaaga acgaatggaa ggcattcgcc
     1681 gccgtctgcg tgcaatggaa aatgacggtg aattggtgtt caccaaaggc aaacgctatg
     1741 cgttgccgga aaaaatggat ttaatcaaag gtacagttat cggccatcgt gacggttacg
     1801 gttttttgca agtggaagga cacgaaaaag gggcgactaa aagcgaagat tggtttattc
     1861 ccaacgccca aatggtaaag gtaatgcacg gtgactttgt gctggcacaa ccaaacggca
     1921 cagaccgcag aggtcggaaa gaagtccgca ttgtgcgagt gttggaggcc cgtaagaaac
     1981 agattgtcgg gcgttttttc cttgaaagcg gtatcggttt tgttgtgcct gatgacagcc
     2041 gcgttaacca agatattctc attccggatg aacaccgtat gggggcgaga atggggcaag
     2101 ttgtggtggt ggaattacag gaaagaaaag cgagttttag ccgcccggtg ggaatcatta
     2161 ctgaaattct aggcgataat ttagcgccgg gaatggaaat tgaaattgcc ctacgcaatc
     2221 acgatattcc gcacatttgg ccggaagggg tagaaaaaca aatccgccaa tttagtagcg
     2281 aagaagtgcc ggaagaagcc aagcaagggc gggtggattt gcgagatctg ccactgatta
     2341 ccattgacgg cgaagatgcc cgagatttcg atgatgctgt atttgccaaa aaagagggca
     2401 acggctggcg gttatgggtg gcgattgccg atgtaagtta ttatgttcgc ccaaaaaccg
     2461 cattagattt ggaagcccta aatcgtggta actcggtcta tttcccgaat cgggttgtgc
     2521 caatgttgcc ggaagtgctt tccaatggct tgtgttcact caatccacag gttgacaggc
     2581 tctgcttggt ggcggaaatg tcggtttcca gcaaagggga attacaagat tatcaattct
     2641 acgaagcggt aatgaactct cacgcccgtt taacttacac taaagtgtgg aaaatgttgg
     2701 aaggtgatga agccttgcgt gaacgctatg ctccattagt gccgcatatt gaagaactct
     2761 atgcaatgtt ccaaacctta atgaaagctc gccataaacg aggggcgatt gaatttgaaa
     2821 ccattgaaaa tcaatttatt ttcaatccgc aagggcgaat tgagcgaatt gagccgttaa
     2881 tccgcaacga tgctcataaa ttgattgaag aatgtatgat tttagccaat attgctgccg
     2941 ctcgttttgt ggaaaaagcg aacgaaccgg cgttatatcg gattcacgat aagccgagtg
     3001 aagaaaaatt gctcagtttt aaatcctttg tgcgtgagtg tggcttaacc tgggacgtag
     3061 gcttagatcc aaccccaaaa gattacggtg tactactgga gcaaattgcc gaacgtgccg
     3121 ataaagagtt gattcaaacg atgttgctcc gctcgctgaa acaagcagtg tatgcggcgg
     3181 ataatatcgg gcatttcggc ttggcattaa ccgaatatgc tcattttacc tcgccgattc
     3241 gccgttaccc ggatttactg ttacaccgtg caattaaata tctgatcgaa aaaggcaaag
     3301 gcaacactcg ctactatacc gatggcggtg gttatcacta caagttagat gatatggacg
     3361 aattcggcga taaatgttct gccaccgaac gccgagctga tgaggcaacc cgagaagtgg
     3421 cagattggct gaaatgcgaa tttatgcaag atcatcttgg cgaagagttt gatggcgtga
     3481 tttcgagtgt taccggcttt ggcttatttg taaaactcaa tgagctttta attgacggat
     3541 tggttcatat ttcaacgctg gataatgatt attaccacta tgatgcggac cgacaacgtt
     3601 taatcggtgg cagtgggatc atctatcgtt taggcgatgc agttagagta aaagtaatca
     3661 atgtgaattt agacgagaaa aaaattgatt ttgagttact caccggtaat aagaaaaccg
     3721 gcaaaaccgc caagaaaaaa gcaaaagcga cagaggttcc tgttttcaaa gagcctaaaa
     3781 cggtaaaaaa agccaaaaaa gtcgctgaga aaaaaccggc aaaatctgca aaaaaacgga
     3841 caaaaacgac cgctagtaaa tcaaggaaaa aatagcaaat ggattacaaa aaacaaattt
     3901 tattcagcct tttatttgct ttagtaattg caataataag ctattttttg tttacctata
     3961 tcccgtttga ttgtagcgat ctgagcaaac tcaacgattc acaacgttta gagtgccgaa
     4021 ctgccgaatt tctgggcgtt tattactggc tgccgcacac taccacgctg tttttcggct
     4081 cggtgttatt atattgggcg gtttatggca tgatttcatt agtgcaagca gtgctcaaac
     4141 gctaacaagc agttttgttt ttgcaaaaag ttgcaaatct catctgaata atccttccaa
     4201 gatcaaaggt ctatcaaaaa agatagacct ttattgtgta aataaactaa aactccttga
     4261 tagatgtatc acatatctat aaaattccag caattaagtg ggaaaaagtg gtaaattgtg
     4321 gaacttttcc ttaaatttaa cctcatttaa aaaggtaaat cagtaaaatg tttcgtggtg
     4381 caagttcaat cagcattgat agcaaagggc ggatcgctat tccgacccgt tatcgtgccg
     4441 agctattgga aaaacatcac gggatcttag tttgcactgt ggatatccgc cagccttgtt
     4501 tgttgcttta cccacttcac gaatgggaaa tggttgaaca gaaattgttg gcactatcta
     4561 attttgaccc tgttcaacgc cgtattcagc gagtaatgca aggctttgct accgaatgtg
     4621 aaatggattc ggcagggcga atactgctta gccctacact tcgccaacac gcacaattag
     4681 agcaacaaat tatgctggtt ggacaattaa acaaatttga aatttggcaa gataaacaat
     4741 ggcaagcaca aattgccgaa gatcttgctt ttggcagttc ggcagaaatt ttagactgcg
     4801 aagcattgaa aaacctgtct ctctagtaat tggaaattga gatgagcgag aacccaaaac
     4861 atattacagt attattgcac gaagcggttg acggcttggc aattaagcct aacggtatct
     4921 atattgacgg tactttcggg cgtggcgggc attctcgctt gattttatcc aaactgggcg
     4981 aaaatgggcg attaatcgca actgatcgtg acccacgagc gatagccgaa gctcaaacga
     5041 ttagtgaccc tcgttttcaa attgaacata cggcattttc tgccattccc gaactgtgtg
     5101 aacgaatggg gttggtgggc aaaattgacg gtattttatt ggatttaggt gtgtcttccc
     5161 cgcagcttga cgaagccgaa cgaggcttta gttttatgcg tgacgggccg cttgatatgc
     5221 gaatggatac caccaaaggc ttatcggcag ccgagtggct tgcccaagtt tcggcagaag
     5281 atttagcttg ggtattgaaa gaatttggcg aagagcgttt tgccaaacgg attgcacaag
     5341 cggtcgtttc ttacaataaa tctgcaaatg aaaaaatcag ccgcactttg cagcttgcgc
     5401 aaattattgc ggatgcggtg ccgtttaaag ataagcacaa acaccctgca acccgttcgt
     5461 tccaagcaat tcgcattttt atcaatggtg aattagacga gctggaaaaa gctttaaatt
     5521 ccgcattaac ggtgttagcc cctgaagggc gtttgtcgat tatcagcttt cattctctag
     5581 aagaccgaat ggtgaaacag tttgtgcgta agcatagtaa aggaatggac gtaccccgtg
     5641 gtttaccgat tttagaagca gagctaacca aaaatattcc gctgaaaacc attggcaaag
     5701 cgattatgcc gagcgaagcc gaaattgaag caaatgcccg ctcacgcagt gcggtattgc
     5761 ggattgccga aaaacgctaa cagtaaatag ggaagaatat gtcgaacgaa cgttatccgt
     5821 tacatcaaat catcattgat gatatcttta gccataacaa agtggctctt ttattgttga
     5881 ttggtgtagt gatttcagca gtggctacga tttggataac ccaccagact cgtttatcgg
     5941 tgagcgaaca agggcaactg attcaagcaa accaaaagtt ggaaagccaa tatactaatc
     6001 tgcaattaga agaaaacagc agaagcagtc gggcaagagt tgatgccgcc gctaaatcgt
     6061 tcggattaca gccgattaaa aaagagcaag aaattatttt agttgaataa aaaatggtta
     6121 aatcagttaa attaaaacgt aagcaagaag cccctgtcgc aaaagcagag cctcaacaag
     6181 cagaaggtaa aaaaacaccg agttttttac ctactcgctt tagagtggtt gtgtttttta
     6241 tgctattaat ggttggtggc ttactagcac aaaccgccta tattcagctt tttgactctg
     6301 aaagattaat taaagaggct aataaccgct cacttcgtac taaagaactc caatttaccc
     6361 gtggacgtat tttagatcgt aacgggcgtt ttctctctat tagtgtgcca atgtactcat
     6421 taacacttga cccgagagaa tactttgata ctaagctgcg tcgttctaac gaaagctggc
     6481 gtgccttagc gattgaaatg gaaacatcaa aaggtaaaat tgaggcaaac gttaataaat
     6541 ttattgaaaa gaaaaatttt tcaaaagaaa aagtagattt tgatccacgt tctattttaa
     6601 atactaaaag tgaaggttat tggactcagc ttgcccaagt tacaggtttg gattacaaca
     6661 gtttaataga aaaagtacgg aataacccta gctcggtttt tttacgttta gataacgaaa
     6721 atttagtaag agaacgccag aaattccaag cgttaagcaa agaaatcggc cgttcctata
     6781 atgacataat ggacgagctt tatagcaaaa accgccagcg attcctttac ttatcccgtc
     6841 atcaatctga agcgatcagc gaatatgcaa aagaactaga tattgatgca ttagtgatta
     6901 aagtcgaatc acgccgtttt tacccgctgg cagaagaatc ttcacagttg atcggtttta
     6961 ctgataaaga tgatatgcac ggttcggaag ggctggagcg tagttttgat tccttactta
     7021 tcggcaaaaa cggtaaacaa attatccgaa aagatgccaa aggcaatatt gtggaaaata
     7081 tccgttcgga gaaacagtac gatccgcaag atgttatgtt aagcattgat gaagaattgc
     7141 agtctatggt gtacggtgaa atcaaaaaag cggtacagga aaataatgcg gaatcaggca
     7201 ctgcggtttt ggttgatgtg caaaccggcg aaattttggc aatggcaaat gccccttcat
     7261 tcaacccaaa caaacgggat agttttaaac ctgaattaat gcgtaaccgt gctattaccg
     7321 acacttttga accgggttca acggtgaaac cttttaccgt attaaccgca ttacaaaatg
     7381 gggtaactta tcgtgatgaa gttattggca ctcgcccatt tgtcgtcaat ggacatacaa
     7441 ttaaagacgt tgctccaagg gacagcctta cgcttaccgg catcttgcaa aaatccagta
     7501 acatcggggt cagtaaatta gccctcagaa tgccggcaaa tgcgttagtg gatacttaca
     7561 cgaaagtcgg tttcggtaaa gacaccggtt taggcttggg tgaacaacga ggtacgaacg
     7621 gtgatcgcaa acgttgggcg gatattgaac gtgctaccct ttcctatgga tacggtttaa
     7681 atgtgacccc attacaactt gcccgtgctt atgcgacttt aggaagtttc gggctgtatc
     7741 gcccactttc gattaccaag gtcgatccgc cggtgattgg cgaacgggtg ctaccggaaa
     7801 aaaccacccg agatgtcgtg catatgatgg aaagcgttgc ggctaaaggc gaaggcggac
     7861 accgtgcagc ggtggacggc taccgtgtag ccatcaaaac cggtacaata cacaaattag
     7921 aaaaaggtaa atatgttgaa aaatatattg cttataccgc agggcttgcc ccggcaagcg
     7981 atcctcgctt tgcattagtg gtattaatca acgaacctaa agcaggcaac tattatggtg
     8041 gctttgtttc tgcaccactg ttctcacgca ttatgggtta caccttaaaa caacgtaata
     8101 ttaagccgga taacttaacg gaaaataccc aaagtgccgt gcgtgaaatt aaattagaac
     8161 gccctatcaa ttaaggttag ctatgaaacg tttactccct tttctgacag aacttgatgc
     8221 ttgggtggaa gaactcaccg agctaaaaca aatgacactt gatagtcgcc aagttcaagc
     8281 cggtgatctc tttgttgctt taaaaggaca tcaagtcgat ggcagaaaat ttattccgaa
     8341 tgcgatagaa caaggagcag caattatttt ggctgaggct gatgaagatc aaccggaaat
     8401 tgagcttgat ccgaaatttg caaaatttaa cctagatcgc accgcttgtt gcaaggttgt
     8461 ctctgttcca cgtctgccaa aattgctctc tgaaattgca ggagctcttt accataaccc
     8521 gtcacacaag ctgactttag ccggcattac cggcacgaac ggtaaaacga ctactgctca
     8581 attattagca caatggcata atttattggg tgggaaatcg gcagtaatgg gtactatcgg
     8641 caacggtttg tacggacaag tgcaagaagc ggtaaatacc accggctctg ccattgaaat
     8701 tcagcataat cttgcgaact ttgttgaact tggagcggat ttttgtgcga tggaagtcag
     8761 ctctcacggt ttggctcaat ttagagccga agccttggat tttgatttag cgatctttac
     8821 gaatttaagc cgagatcacc ttgattacca caacacgatg gaagagtatg cccaagccaa
     8881 attccgtctc ttcaacgaat taagcaccaa agctcaagta attaacgccg atgatgaaat
     8941 cgggcgtgaa tggctgacac aattaccaaa tgcagttgcg gtaagcaccg atccgaagtt
     9001 tgcaggcaac caccaatttg tcaaagccac tgcggtaaaa tttaccctac aaggggcaag
     9061 tattgcgttt gaatcaagtt ggggcaatgg cgagttacat agccgtttaa tcggggcttt
     9121 caatgtaaat aatctgctta ccgcctttgc cggactttta gctcttggct ttgatattca
     9181 agaactggtt aaaaccgccc cgaatttagt tggcgtagca ggtcgaatgg aatgcattac
     9241 cgctccaaat aagccaatgg taattgtgga ctatgctcac acccctgatg ctcttgaaaa
     9301 agccctacaa gcggctcgtt tacattgcga aggcgaactt tggtgcattt tcggctgtgg
     9361 tggagaccgt gacgccggca aacgcccatt aatggcgacg attgccgaga aattaaccga
     9421 taaagtgatt gctacagacg acaaccctcg caccgaagat aatcaaaaga ttatggcaga
     9481 tattgtgaaa ggttttagta aaccccaaca aattatccac aatcgtgaag aggcaattaa
     9541 aaccgccatt gagcaagcta aagccacaga tgtgattcta atcgcaggca aaggtcacga
     9601 agattatcag attattggca cagagaagtt acatttttcg gatcaggaaa cggcaaggaa
     9661 atatctgttt taatgtcact ttctttgcac aagcaaagaa aagtgacagt gacaaataca
     9721 aaatgctcgt aattaatttt ataaagaaaa agataaatca atgataaaac taaccaccca
     9781 acaaatcgct gaaatcctaa atgccaaatt gatcggcaat ggcgaaactg tggtagaaac
     9841 caccagcacc gacacccgac aagcggttga aaacggctta ttttttgcat taaagggcga
     9901 gaactttgat gctcatcact atcttcaaaa tgcagtcgca caaggttgtg tagcggttgt
     9961 ggttgagcgt gagtgtgaaa ttgatgtacc acaacttgtg gtggcagata cccgtttggc
    10021 attgggggaa ttggcgaaat ggttaaaagc taagctgaac ccgaaaacgg tagcgatgac
    10081 aggttcttcc ggcaaaacaa ccgtcaaaga gatgacggca aaaattctgc aaaaaatgac
    10141 cgcttgcgaa caggaagtgc tttatacttt tggtaactta aacaatgact taggtgtacc
    10201 gatgaccttg cttcgcttaa ccgaacagca taaatatgcg gtcattgagc tgggggcaaa
    10261 tcatatcggt gaaatcgctt ataccaccgc tatcgctcag cctgatgcct gcttggtgaa
    10321 taatgtggct gcggcgcatt tagaggggtt tggttcatta gcaggcgtgg cacaagccaa
    10381 gggcgaaatt tatcgtggct tgaaagaaaa tggcaaagcg attgtcaatt tagcttttta
    10441 ttatccgcaa tggcaaaaag agattggctc acgggaaatg caatctttct cttatatcgg
    10501 gtcagattcg gattcttacg cagattactg ggcggaagat gtagaactgc atttaagcgg
    10561 ttcacgcttt acgctacata ccccacaggg cgaaattgct attgatctgc cttatttggg
    10621 caaacataac gtgagcaatg ccttagcggc aacttcgctg gcgatgtctg ttggggcaac
    10681 gctagaagca gttaaggcag ggcttgagca gcgttcaatg gtaaaagggc gtttgtatcc
    10741 ggtgcaaatt aacgaccact gtttgtttat tgatgacact tacaacgcca atgtggattc
    10801 aatgaaatcg gcgatttctg ttttggaaaa ttatccggct tatcgtattt ttgcagtggg
    10861 tgatatggcg gaattgggtg aagaaagtag caaatgccac caagaagtag cggattttgc
    10921 caaaaatgca gcattagatt taatcgtgag ctttggaaaa gaaagtgcgg taatcagcca
    10981 acaggcagaa catcatttta ccgataaaca gcaaatggtt gatttcctac tgccgattat
    11041 tttgcaaaaa atcgaacaaa aacaaccgct tgtattattg gcaaaaggct ctcgtagcca
    11101 gaaaatggaa accgtgattt ccgaattgtg taaaccgttt aacctaaatt tctaattatt
    11161 tttaaggact aagcaatgtt agtttggctt gcagaactat tggttcaata taacacagcg
    11221 tttaacgttg tctcttatat tactttccgt gccattatgg cgttattaac tgcaatggga
    11281 atcgggcttt ggatcgggcc gaaagtaatt cgccgtttgc agatcttaaa atttggtcag
    11341 gaagtgcgta atgacggacc ggaaagccat ttcaaaaaac gtggtacgcc aacaatgggc
    11401 ggagtgatga ttctcgcagc gattggggtg agtacattgc tttgggcaga tctgcgtaat
    11461 tcctatgttt ggttcacgct gtttgtgcta tttggctacg gtgcagtggg ctttgttgat
    11521 gattatcgta agatctctcg taaaaatacc gatggtttga ttgcccgttg gaaatatttt
    11581 tggttatctg ctatcgcatt agtggcggtg tttggtatgt atgcggtggg taaagatacc
    11641 gctgcaacac aattagtcgt gccgtttttt aaagatgtga tgccgcaact cggtttattt
    11701 tatattgtgt tatcctattt cgtgattgtc ggcaccagta atgcggtaaa cctaacggac
    11761 ggcttagacg ggcttgcgat tgtgccaact attatggtcg catctgcctt tgcgttaatt
    11821 gcgtgggcaa ccggtaactt taattttgca caatatttac atattccttt tattccaaat
    11881 gccggtgaat tggttatttt atgtactgcg attgtgggag caggtttagg gttcttatgg
    11941 tataacacct accctgcaca ggtgtttatg ggcgatgttg gctcactttc gctcggcggt
    12001 gcattaggcg tgattgcagt attagttcgc caagaattat tattggtggt aatgggcggt
    12061 gtatttgtgg ttgaagccct ctcggtgatt ttacaagtag gttcatacaa attacgccaa
    12121 aaacgtattt tccgaatggc tccaattcac caccattttg aactcaaagg ctggtctgaa
    12181 ccacgggtta tcgtgcgttt ctggattatc acgctaatgc tggttttagt cggcttagta
    12241 acattgaaat tacgttaatt gattgaataa ggtgcattga gataatgtac cttatgttat
    12301 ttttaaatta cagaataaat tatgcaaaaa caatacgaaa acaaaaccgt tactattatc
    12361 ggcttaggta caacagggtt atcttgtgtg gatttcttta agaaaaaaaa tgccaatatc
    12421 caagtgattg acacccgagc aaacccagcc ggagcggata aattggacgt aactattccg
    12481 ttgcataccg gtgggttaaa cagtgaatgg ttgctgaaat ctgatcttat tgtaattagc
    12541 ccaggtttag ccgttgccac acctgaaatt cagcaagcga ttcaagctgg tgttgaaata
    12601 gtcggtgatg ttgaactgtt ttgtcgggaa gcgaaagctc cgattattgc gatcaccggt
    12661 tcaaacggca aaagtaccgt aacgacttta actaccgaaa tggcgaagca agcggggatt
    12721 aaagtgggaa tgggtggcaa catcggtgtg ccggtattaa gtttgctgga gggcgattat
    12781 gacctttttg tgctggagct atccagcttc cagcttgaga caacttattc actgaaagca
    12841 aaagctgcca ctgttttaaa tattagcgaa gatcatatgg atcgctacga ttcgattgcc
    12901 gactatcgcc aagcgaaatt gcggatttac gataatgcgg aaaatatcat cgtaaacggt
    12961 gaagatgccc aaacttaccc gatacaagcg gtcaaaaaag tgattcgttt tgcagaacaa
    13021 aatgcggaat atagcctacg caatcagatg ctttattcag gcgatgatgc aattatccaa
    13081 actaaacaga tgctgattag cggccgtcat aatgagatga atgcgttagc ggcgatggca
    13141 ttagcagaag cggcgaatgt tcctcgtgaa ggcattgtga aagctttgca aatttatggt
    13201 ggtttgccac accgtttcca agcggtggca accaatgatg gcgtgcgttg ggtcaatgat
    13261 tccaaagcga ccaatgtggg tagcacggta gcggcattaa acggcttgaa tattgccggt
    13321 acactttatt tacttttagg tggtgacggc aaggctgcgg atttttccga actcaaacca
    13381 ttaattaata agccaaatat tgtctgctat tgtttcgggc aagatggagc ggcattggca
    13441 gaattaagcg aacaaagtgt gttggttgaa acgatgcagc aagcaattga agcaatccgc
    13501 ccacaactga aaaaagacga tatggtactg ctttctccgg cttgtgccag tttagatcag
    13561 tttaaaaact ttgagcaacg tggcgaggta tttatggcat tagcacaagg acagcacgca
    13621 taatgttaga aagaattaag tcggaatggg ataaatgggt cagattaacc ccttctaatt
    13681 cactttatga cagaactcta atttggctat ttttaggcct gctaattatc ggttttgtga
    13741 tggtaacatc tgcttcactc ccagttagca caaggttgaa taacgatcca ttttattttg
    13801 cgattcgaga cgggctgtat attattgctt caattatctt ttgttatgtt tttgtacaaa
    13861 tcccaattga aaaatgggaa aagcataact tggcactgtt ttttatttca atcggttttt
    13921 taatcgccgt actcattttc ggccgctcaa ttaatggtgc agtgcgttgg attccgttgg
    13981 gtattctcaa ctttcagccg gcagaattag ccaaattagc ggtcatttgc tattttgcca
    14041 gtttctatgt acgtaaatat gatgaaattc gtaaagaaaa agccagtttc tggcgaccgg
    14101 cagtgatttt attcttattc ggcttcttgc tgattttgca gccggattta ggtagtacct
    14161 ttgtattatt tgttctcact ttctcaatgc ttttcattgt gggggcaaaa attatgcaat
    14221 ttatgttttt aggcgtggtg ggaacggtgt tatttgcggt gttgatttta acctcggaat
    14281 accgtttaaa acgtgttacc tcctttatgg atccgtttgc tgatgcttat ggcgacggtt
    14341 tccagctctc gaactcacaa atggcatttg gacaaggtga attttgggga caaggtttag
    14401 gtaattcggt acaaaagcta gaatatttgc cagaggcaca tactgacttt gtaatggcgg
    14461 ttattggtga agaatttggt ttctttggta ttgcttgcat cgtactttta cttattctat
    14521 taacggtgag agccttaaaa atcagtaaag aatctttggt gctggaagag cgttttaaag
    14581 gctatatggc atttgggatt gcgatttggg ttttcctaca aggcttcgta aacttagggg
    14641 tggcatcagg tttgctgcca acgaagggct taaccttccc attagtgagt tatggtggtt
    14701 cgagtttggt aattatgtcg attgcgattg cagttttgct acgaattgac cacgaaaacc
    14761 gtgccgagcg aatcgggcac gcaaaaatta aaaccatcta gccgagcggt ctgcaacgtt
    14821 acaatttgca aaattttaca aaaatgagac cgcttgtatt agctggaaag cataaaaatt
    14881 cagtaaaata gcacgtttta gaataaaaaa gagttgaaaa tggcgaagaa attattagta
    14941 atggcaggtg gaacaggggg gcacgttttc cctgcaattg ccgtagctcg agagctacaa
    15001 aaacaaggct ggcaaatccg ctggctgggg acgaaagacc gaatggaagc cactcttgtg
    15061 ccgaaacacg gtattgagat tgattttatt gaaatctcag gcttgcgggg taaaggcgtt
    15121 gcagcattgt tgaaagcacc gtttgctatt tttaaagcgg tgatgcaagc ccgcaaaatc
    15181 attaaaaatt atcagcctga tgcggtgttg ggaatgggcg gttatgtgtc cggtccgggc
    15241 ggtattgcgg ctaaattatg tggcgtgccg gtgattttac acgagcaaaa cgcagttgcc
    15301 ggcctaacca atgtgtggct atccaaaatt gcccgcaagg tgttacaggc tttccctact
    15361 gcttttaaag aggccgaagt agtaggtaat cctgtacgtg aagatttatc tgctcttccg
    15421 ctgccgaatg aacgtttcgc caaacgaggc tacccgatta acattttagt aatgggtggc
    15481 agccaagggg caagagtgat caaccaaacc gtgccggaag tggcaaaagt gttgggtaat
    15541 aatgtgttta tcagccacca agtcggtaaa ggtaatttag ccggtatcga agaagtctat
    15601 caacaaaccg gcaatgggat tgcttccgag tttattgatg atatgaaagc cgcgtatgag
    15661 tgggcagatt tggtgatttg tcgttccggt gcgttaacgg tgtgtgaaat tgccgccgta
    15721 ggtctgcccg ctgtgtttgt gccgttccaa cataaagaca ggcaacaatt tttaaacgct
    15781 aactatttgg cggcagatgg agcagcagtg attatcgaac agagcgattt cactccaaag
    15841 gcattacagc aagccttaga acccttaatt gccaatcgcc aactcttatt ggatatgtca
    15901 atgcgagcca gagcaaaatc caccccaatg gcagcaaaac gtgtagcgga agtgattatt
    15961 caagaagcga attaacttac tacaagcggt cgtttttggt aaaaattttg caaaatgtaa
    16021 ccgcttgcga atagattaaa aggacaataa atgaaaaatt tccaagataa agtcaaaaaa
    16081 ttagtgcctg aaatgcgtcg tgtcagccaa attcatttta tcgggattgg cggagccggt
    16141 atgagcggca ttgcggaagt gttattaaac gagggatacc aaatttcagg ttcggatatt
    16201 gcagacagag cggttacaca acgtctatcc gcagccggag cgaaagtctt tatcggacac
    16261 caagcagaaa atattgctgg ggcgagtgtg gtggtggttt ctactgcgat tgatgaaacc
    16321 aaccctgaaa ttattgccgc tcgagaagct cgaattccgg tgattcgccg agcagaaatg
    16381 cttgccgaaa ttatgcgttt tcgccacggc attgcgattg caggcacaca cggtaaaacc
    16441 actacaaccg caatgatttc aatgatctac accgaagcaa aattagatcc gactttcgtg
    16501 aatggcggtt tggtaaaatc taccggcaaa aatgctcatt tgggggcgag ccgttattta
    16561 attgccgaag ccgatgagag tgatgcttct ttcttgcact tgcagccaat ggtgtcggta
    16621 gtaaccaaca ttgagcctga ccatatggac acctatggtg gcgattttga gaaaatgaaa
    16681 gagacttacg tacgattttt gcgtaactta cctttctacg gcttagcggt aatgtgtgcc
    16741 gatgatgaaa cggtaatgga aattgctcct cgtgtcggca gacaagtgat tacctatggc
    16801 tttagcgaaa atgcggatta ccgaattgaa gattaccagc aaaccggctt ccaagagcat
    16861 tacaccgtag tttgcccaag cggtgaacga attgatgtgc tgttgaatgt gccgggtatg
    16921 cacaatgcct taaatgcaac cgctgcgtta gcggtggcaa aagaagaagg gattggcaat
    16981 gaggcgattt tagctgcttt agctgatttc caaggggcag gacgccgctt tgaccaatta
    17041 ggttcattta ttcgcccgaa cggcaaaatt atgttagttg atgactacgg ccaccaccca
    17101 actgaagttg atgtaaccat tcaagccgct cgttcaggct gggaaggtaa acgtgttgtg
    17161 atgattttcc aaccgcaccg ctattctcgt acccgtgatt tattcgatga atttgtacaa
    17221 gtactgtcta aagtcgatgc cttgatttta ttggatgtgt atgctgccgg cgaagctccg
    17281 attgtggggg cagacagtaa agccttagcc cgttcaatcc gtaatttagg taaggtcgat
    17341 ccgattttgg tttcagatac cgatcaactt ggcgaggtat tagatcaaat tattcaagac
    17401 ggtgatttaa ttttggcaca aggtgcgggc agtgtaagcc gcatttctcg tggcttggct
    17461 gagagctgga aaaattaaat actctctccc cttgcgggag agagaccgaa aaatacttcg
    17521 ttcagaagta tttttcagag agagggtata tatttaaagg atttcaaatg agtattaaaa
    17581 acgaaaaaat tgccgtctta ttcggtggcg tttcgcaaga gcgtgaagtt tcactgaatt
    17641 ctggtgcagc ggtaacagct gcattaaaaa gtttaggcta taacgtagaa ggcattgata
    17701 ctaaagaatt cccgattgaa aaattaaaag aaaaagggat tcagcgtgta tttaatattt
    17761 tacacggcgg tattggcgaa aatggcgtac tgcaaggtgc gttagagcaa atgggtattc
    17821 cttacaccgg ttgtggcgta atggcatcag cagtgacgct ggataaattc cggactaaat
    17881 tattatggaa tgcagtaggt ttaccaacag cggatatggt ggtggttcag cgtggacaag
    17941 cggtcgattt tgaccaaatt attgcaaaat taggcttacc ggtatttgtg aagtcttcgt
    18001 gtgaaggctc aagtgtgggt gtattcaaag taaaaaccaa agaagaattg gcaccggcaa
    18061 ttcacgaggc attaaagttt gattccattg tgttggtgga agaattttta gccggtaatg
    18121 aatattctgt accggtatta gacggacaag ttctaccggc ggtacaagta attccggacg
    18181 gcgaatttta tgactaccac gcaaaatatg tgtcggataa cacccaatat gtagtaccgg
    18241 cattaaatga cgaacgccaa gcagaggtcg ccaaattagt gaaagcggct tacgatgtgg
    18301 ttggctgtcg aggttggagc cgtattgatg tgatggaaga tggcgaagga aaattccgct
    18361 tggttgaagt aaataccaac ccgggaatga ccagtcacag tattttccca aaatcagcag
    18421 caacagttgg tatttccttt gaaaaattag ttgaacgcgt attggagtta agtgcttaat
    18481 gggcatcttc cgcaagaaga acagcacaaa tgtgcgtttg aatgttctta atccaaaaac
    18541 acctgtttct ccacgtaaat ggctagtatt cattaagcca ttaattgtgt tattatgtac
    18601 actatttgta ttcggtgtgt atagtaattg gcaaaatata ctcgaaagtt gggataaaac
    18661 accgattcgc gcttatgcat taacacataa aacacaattt acaacaaatg cagatattcg
    18721 tgaaactttg tctaaagaac ctgctctaaa aggatatttc ggacaaaata ttcaagaggt
    18781 caaagataag cttttatcta taccttgggt taaagatgtg gctgttcgta aactttatcc
    18841 cgaccgatta agtattactt tgctagaaca taaacccgtt gcaatttgga atgatactaa
    18901 ctttgtctca gaacaaggaa ccgtctttag tttaccgaaa gacagaatcg ataaaaatgg
    18961 acttccatta ctctatggtc cagatacgga aggaaaaagt gtattagagg catggaataa
    19021 aatccgagct gatctaaatg cccgcaactt ggatttaacc tccgtttctg tagataatcg
    19081 aggttcttgg acaattaggc tttctaatca agtagaatta agattaggaa gaggggaatg
    19141 gacttctaaa attgatcgtt ttgtaataat ctttcctgaa attcatatac ctgaaggtaa
    19201 tagattagct tatgttgatt tacgttatcc acatggcgcg gcggttggat ttagtcctat
    19261 aaaaaattaa actcagtgaa cacaaaaaca ttatgacaaa agcagtagaa tcaaaaatta
    19321 tcgttggtct tgacattggg acatcaaagg ttgttgccgt tgttggtgaa gtattacctg
    19381 atggtgtaat taatgttatg ggagctggcg tttgcccttc taaaggagtc gatcgaggtg
    19441 gcgttattga tcttgacgct gccgttaatt ctattcaaag agccattgag caggcggaat
    19501 ctattgccga ttgtcaaatt atgggtgtaa cactggcaat ctcaggtcag catattgttg
    19561 gtttaaatga agcaggatca tcaacattat ctggaggtat tgttacccaa gataatattg
    19621 aaagtgctgt ggatatggca aaggcaatta aattacaaga tggcttagaa acattacaca
    19681 tgattccaca agaatatcac gtggatagat tgcctgcgac taaaaatcca attggcttat
    19741 cgggtatgcg tttacaagtt caagcacact taattgcttg ccatcaagct tggttaaaaa
    19801 acttaaaaaa tgcggttgaa catgctaaat taaaagtcga tcaagttgta ttttcaggtc
    19861 ttgcctctag ctattctgtt ttaactgaag atgaaaaaga gctgggtgtt tgcttaattg
    19921 atattggtgg cggtacgatg gatgtactag tttataccga tggagcatta agatacagta
    19981 aagtcattcc atttgcaggt aataacatta ctgattattt ggctcgtgta tttacgacat
    20041 ctcgccctga agctgaaagc cttaaagtag gttatggtag cgcaatttcg cctccaaccc
    20101 ataattcaga taaaaaaatt gaggtggcag gactaggtgg acggatggca agaaccttta
    20161 cccgagctca ggtggcaacg gttacctcac aatgttataa cgatttgcta aaagttgttg
    20221 aagaggaatt aacccaatta cgccatgaac ttttccaaaa aggaataaaa caagagttaa
    20281 ttgccggtgt agtcattact ggtggtggct cacaaattga agatattgta gaatgtgcta
    20341 aatcaatttt tggttcacaa gttcgtgttg gatatccact caatattact ggcttaaccg
    20401 attatgtgaa taaaccacaa tatgcaacag cattagggct tttacaatat agccactata
    20461 attatactga acaaggtaca agtacagatt ctaatgaaga tatctttgct cctattggtc
    20521 gtggaaccaa gaaaatcatt gacttcttca aatcaaaatt ttaatagtgt taaaataaac
    20581 acgcagggag ataaaaatgt ttgaacctat tattgaacaa acgcaagatg ctatcattaa
    20641 ggtagtgggt gttggtggcg gtggcggtaa tgccgtagac agaatgtctc gctctgccga
    20701 tgatattaaa ggtgttgaat tctttgatgt aaatactgat gctcaagttt taagaaaaag
    20761 aaccacacgc caaactatcc aaattggagc aagtacaact aaaggtttag gtgcaggtgc
    20821 tgatccaatg gtaggaaaac aagctgccga agaagatcgt gaggctatcg ctaatgcatt
    20881 aaaaggtgca aatatgacct ttattgctgt tggtatgggt ggaggtaccg gtaccggagc
    20941 agcccctgtt gttgctcaaa ttgccaaaga gcaaggctca ctaactgtag gcgttgtaac
    21001 aaaacctttc cgcttcgaag ggcctcgccg tatgcgcttt gcagaccaag gcattaaaga
    21061 gctttctcaa tatgttgatt ctcttattat aattcctaat gataaattac gtggtttagg
    21121 taagcaaaca acagcagtag atgcctttgc cgcagcaaat gatgttttaa gtaactgtgt
    21181 gcttggtatt accaatatga ttaccagttc gggcggttct accggtgcag atattaacgt
    21241 ggacttcgcg gatgttcgta cagtaatgtc tggtaaggga cacgctatga ttggtacagg
    21301 ttttgctgaa ggtgaagttg gtgaaggacg agcggaaaaa gcaatgaatg atgccatttc
    21361 tagtccatta cttgaaaatg tggatatttc aggtgcaagt ggtatgctca tcaacattag
    21421 tgctgggaca gatttcttat tagaagaagt ttatgctatg atggacttaa tttatggctt
    21481 tgcaacagaa gatgccgcaa ttgtatttgg ttgtaactat taccctgaaa tggatggaaa
    21541 agtgagtgtc actcttgtcg caacaggaat aggccaacct gaggaagctc tacatatgcc
    21601 acataaggct cagccagtat atgcacagca aggaaatcat gttcaagcta cacagcctac
    21661 tcagccaaac catgcggtac aacaaccaaa ctttactcaa ccgtcaagtt atggtcagcc
    21721 aaatcaattt agtggtcaac cacaaccact acaacaacct gtggctccaa aaccacaatc
    21781 ggttgataca gcacaaattt ttaacccaaa ttcaattccg ggttttatga gaaatagtca
    21841 ataacttcca ctcatttcgg aagtaaggac aaaatatgac taaacaaaga acattaaaac
    21901 aagcagcaaa agtaacaggt attggcttac atagcggcaa aaaagtaacc ttaacgttac
    21961 gccctgcccc agctaataca ggagttattt atgcccgtac cgatttagag cctgtcgttt
    22021 atttccctgc aagcgcagac tctattcgtg atacacaatt atgcacatgt atggtaaatg
    22081 atgatggtgt tcgtatttca acagtagaac atttaaacgc agcaatgtca gcattaggct
    22141 tagataatgt tattgttgaa gtagatgcac ctgaaattcc gattatggac ggtagttcaa
    22201 gcccatttat ctatctttta ttagatgccg gcatcgaaga acaagatgca ccgaagaaat
    22261 ttattcgcat taaagaaaca gtacgcgttg aagaaggcga taaatgggcc gaattaaaac
    22321 catacaataa aggttttaaa ttgaacttca ctattgactt tgagcaccca ttaatcccca
    22381 aaaatgtagg gagctattcc attgattttt ctgctgagaa ctttattcag caactaagtc
    22441 gagcgagaac ctttacattt atgaaggatg tagaatactt gcaatctatt ggtcttgcat
    22501 taggtggaag ccttgataat gctattgtat tagatgatta ccgaattcta aatgaagacg
    22561 gtttacgatt taaagatgaa ctggttcgcc ataaaatgct ggattcgata ggtgacttat
    22621 atatgtgtgg ttataatatc ctaggtgaat ttagtgcata taaatcaggt catggcgtta
    22681 ataataaatt attaagagct gtattggaaa ataaaaacgc ttgggagttt gttacctttg
    22741 aagataaagc agaagtccca caaggttacc aagtacaaca agaaattttt atttagtcta
    22801 aatggcttaa aagacgaaca gaaatgttcg tcttttattt tgcaaatttt ttctaatatt
    22861 taaccgcttg caacccgcct cattagcgat tttttcgccc ttatctattt actgtttaaa
    22921 aaaacagttg tactatatac ccatacagtt aatcttgtgt ggtgtattat gcttactcaa
    22981 cttactatta ataattttgc gattgttcgc catcttattc ttgaattaaa tgaaggtatg
    23041 tctgttatta ccggtgaaac cggtgccggg aaatcaatcg ctattgatgc cttagggcta
    23101 tgcttgggtt atcgttctga aagtgggatg attagaaacg gttcggacaa agcagatata
    23161 agtgcaacct ttactatgca gccacatagc cctgcttatt tatggctaca agagcatgaa
    23221 ctgcttgatg aagataatcc tcaagagtgt atcttacgcc gtatgattaa tattgatgga
    23281 cgctcaaaag cctttgtaaa taaccgatca ttacctgttt cacaacttcg agagttgggg
    23341 caatatctta ttcatttaaa tgggcaacac gcaccacaat tactcctaaa aaacgaatat
    23401 caacttgaaa tagtcgatca ttatgccacg ttacagcctc tattgatgaa gatggcaaag
    23461 caatatggga attggaaaga actgaataag caggtaaaaa atttccaaaa acagtgccaa
    23521 gaaaatgaag ctcgcaaaca gctattagaa tatcaagttg aggaattaga tgaatttgca
    23581 attaaagcaa acgaatttga agaaatggaa gagaaataca atatcttgtc ttcttccgag
    23641 gagcttactg agctttcaga tgaaatcaca cgcttattaa gtgatgatga aatcaatgca
    23701 aacagtctga tttataaagc gttacgcaat gcagaaaact tggctgaggt tgatagccaa
    23761 tatcagggca ttttagatat gcttaatgag gccctgattc agattcaaga agcaagctcc
    23821 gaagtggctc aattagccag taaaattgaa caagatccgg aattactaca ggagctggat
    23881 aatagaattg cacaaactat atcgttagca cgcaaacacc atattcaacc gaatgaatta
    23941 tggcaatatc acgcgacatt aaaagcagaa ctacaggaat tactggattt tgaagataat
    24001 gaagaacaac tcattcaaaa tgaaaaacaa gcttaccaag agtgtataac attagcagaa
    24061 gaaatttacc aaaaacgtct agatgccaca cataagctct ctgaacaagt aacaaaacaa
    24121 attaaacact tagcaatgga aaacggtgag ttctttattc atatagaaca taatcttgaa
    24181 aaaatgtctg caaatggtgc tgactatgtc gagtttaatt tacgcagcaa tttaggacaa
    24241 caaccccaac cactggcaaa aattgcttct ggtggtgagc tatctcgcat ttctctggca
    24301 gtacaagtgc ttaccgcaaa taaactttct acgccaacta ttatttttga tgaagttgat
    24361 gttgggatta gtggctctgc tgccactaca gtcggaaagc tactgcgtaa gctaggcgaa
    24421 aaatgccaag tattatgtgt aacccattta cctcaagtgg caagttacgg caaccagcag
    24481 tttaatgtac aaaaatatgt agaaaataat caaactgaaa cgcaaatgac attactcaca
    24541 gaagcagagc gtattcacgc cttggctcga ctattaggcg gaagcgaaat caccgaaacg
    24601 gttttagcta atgcaaaaga aatgctaaat ttagcgaaaa ataacgaata gccaaaaaga
    24661 aaaatcgcat caatgaatat tgatgcgatt atgaatttgg tggaggtaaa cgggatcgaa
    24721 ccgatgacct cttgcatgcc atgcaagcgc tctcccaact gagctatacc cccttaatac
    24781 aagcggaata atatgctttc cgcttgaaga tgtcaatggc tttaatcaag caaggtgttg
    24841 attgattaaa aactagctat tcgcctgaat aaactcaatc gctttttgta agcgagctaa
    24901 ggttctttct ttaccgacta atttggcggt gatatccatt gacggcgatt gcccacttcc
    24961 tgttaccgct aaacggaacg gcataccgac tttgcccata ccaatttcca gctcggcagc
    25021 ggtttggttc attgcctcgt ggatattttc caccgtccag tcattgcaag cggtcaattt
    25081 ttccaataat tttgcaagtg gagcaacggc ttcggctttg aagtttttag cagcagcttt
    25141 ttcgtcgtaa gccttaaatt cttgatagaa atagcggctg ctttcggcaa gctcttttaa
    25201 ggttttgcaa cgttcgccca gtacagggat aatttcttcc aattttggac cgcttgtgaa
    25261 atcaatgcct tgagtttgca tatgccattc aagatgtttt gccacataac ttggggcaag
    25321 actgcggata tagtgttggt tcaaccattg taatttttca gtgttaaatg cacttgccga
    25381 tttactgact gaatgaatgt cgaacagctc gatcatttcg tcacgagaga aaatctcttg
    25441 gtcgccgtgt ccccagccta aacgcacaag gtagttaatt aaggcttccg gtagatagcc
    25501 gtcatcacga tattgcatta cgctcaccgc accgtggcgt ttggagagtt tttgcccgtc
    25561 atcgccgttg atcattgaaa cgtgggcgta ggttgggatt ggtgctccta atgctttcaa
    25621 aatgttgatt tggcgtggtg tattgttgat atggtcttca ccccgcacaa cgtgagtaat
    25681 gcccatatcc cagtcgtcca ccaccacaca gaaattgtag gtcggcgagc cgtcagtacg
    25741 gcgaataatc aagtcatcta attcgctatt ggcgatttca attcgcccac gcaccgcatc
    25801 gtcaaatact accgagcctt cggtcgggtt tttgaaacgc acaacatgtg gttggctcgg
    25861 gtcgttttgc ggattgtgta agcagtgctg atcgtatttc gctttctctt tatttgcttc
    25921 ttgctctgca cgtacttcgt ctaaacgttc tttcgagcag tagcaacggt atgccgtgcc
    25981 ggcttctaac atttggtcga tcacttggtt atagcgatcg aaacgcttgg tttgaaagta
    26041 tgggccgtgt tcccacgcca aatccagcca ttccataccc tctaaaatag cggcggttgc
    26101 ctcaggggtt gagcgttcaa gatcggtatc ttcaatacgt aacacaaatt caccatcatt
    26161 atgcttagca tataaccaag agtagagcgc ggtcctcgcc ccacctacgt gcaagtagcc
    26221 agttgggcta ggtgcaaaac gggtacggac ttttacattc ggatctaagg ggaaaagggt
    26281 ttcaattttc attttatatt gcctaattga tgattcttaa gaaaaacttg tctattttac
    26341 tacgcttgag ggtgaatcat agtaaaaaat taaatttgcg gggtaaaaaa tcagcaaacg
    26401 gggtaaaaag aacaaaaagg gtttgacgaa acagcaaaaa tctctataat tcacttccac
    26461 aacagcgggc gattagctca gttgggagag cacctccctt acaaggaggg ggtcactggt
    26521 tcgagaccgg tatcgcccac cacttcaact taagtgtttc gttgttgtat cacaatctaa
    26581 ttttgaaaac gggcgattag ctcagttggg agagcacctc ccttacaagg agggggtcac
    26641 tggttcgaga ccggtatcgc ccaccacttc aacttaagtg tttcgttttc aaattccctt
    26701 tgattttaag tctcaagtgg gcgattagct cagttgggag agcacctccc ttacaaggag
    26761 ggggtcactg gttcgagacc ggtatcgccc accacttaag actaaaatca taaacttcaa
    26821 atggcgattt ttcgtatctg aagggcgatt agctcagttg ggagagcacc tcccttacaa
    26881 ggagggggtc actggttcga gaccggtatc gcccaccatc tatttagtat caaagcctaa
    26941 aacttatatc ataatctaat aaccccttta aagtcataac taaaaaggga aaataaattc
    27001 agatggcctg ttgcatctgc ataaatgcct ttctacgtgc agaaattaat ctttttctat
    27061 ctttaatttg cttaatttga cgcaggcgta atttccttgt cagtagggat ttctttttct
    27121 tcaattttct cataagaaga attctcctgt tttatttgac catctggtaa attttcttgt
    27181 tcttgcatct tttgctcttc aactcgttga taattaataa tactaccata ataacgccgt
    27241 atattttcaa catactgtaa tgcttcatat cctcgagcat agccgtattt tagattagca
    27301 taataacgtt tttccgcaag caatggcaga ttctttttaa catcaagcca attatccgga
    27361 tcaccaccaa gttgttttgt taatcgacga agatccaaca agtgccccaa tcccatatta
    27421 tacgctgcta ggctatacca aattctatct gcttgaggaa tagtttccgg catttgtctc
    27481 attagcatat gtaaatattc agatcccgct ttgatacttt gtgcagggtt agtcctatca
    27541 gaaattttca ttctatctgc cgtatctttt gtcagcatca taattcccct cactccggtt
    27601 ggagaagttg cattaggatc ccaatgagac tcctgatagg cgatagccgc cagcatttgc
    27661 cattctaatt cacctttatg tgcccgaaaa aggggttcat atttaggtaa aatagtttta
    27721 attgcattca aatagctctg aatatcaaca taatcaaact tagctaaatg attaaaatat
    27781 ttctcttcta ttcttgatat cagcccagtt tccatagaat aagacataaa atccagcagc
    27841 acagattgca gttcactata agaactattt gctaaatacc ataacactgg gttttcatca
    27901 gttaaatcaa accctacggc taaattagga ttaatatgct gtgcagcaga aatatcaacc
    27961 gacattgcaa ttgtatgtga aatttcacct tgtgcgactt ttaacaatag ctcttcttgt
    28021 gtaaatttat ccgagatctg ccattttagt tccggattat ccaccttaag ctgatttaat
    28081 attggaatta ccgcagaacc ggctggaata atcacatttt cccgcaaatc ggaaagtcga
    28141 tatggtctag cacttccctt tttataaaca agctgccaag aagccgaata ataggaagga
    28201 cctatctgaa attcatcgtt taattcagaa tggtaaagca gcccagcggc agcaatatca
    28261 acatcattat ttcttaaggc ctcaaacaat ttctcgctac tatcataagt tttaatatca
    28321 agattgacct ttaaatattg agcaaaggct gttgctaaat catattcaat gccacttttt
    28381 ccttcaggac caacaaagta agaaagtgga tggttgatca ttccaacctt taacgttttg
    28441 ctttgctgaa tttgtgtata atgatttgct tctgaatgaa tgattttttg ccaaggaaaa
    28501 atcatatccc aagcccacag taacaatgct acggcaataa aaaggcgagc aattaatcct
    28561 ttcaatttaa aatcctcatt atttcaagct aagcattaag cctatctgaa attacaaatt
    28621 aacggaataa tactttatag tttaattatt tattaa
//
LOCUS       DANLXS010000010        26269 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.010, whole genome shotgun sequence.
ACCESSION   DANLXS010000010 DANLXS010000000
VERSION     DANLXS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 26269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26269
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.010"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            26..1942
                     /gene="iolC"
                     /locus_tag="PXD71_000354"
     CDS             26..1942
                     /gene="iolC"
                     /locus_tag="PXD71_000354"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL6290515.1"
                     /translation="MATVNHQDRPLDLICLGRVAVDFYGQQIGSRLEEMTTFSKYLGG
                     SSGNVAYGTAVQGLRSSMLARVGDEHMGRFLREELQSVGVDTSHLITDKERLTALVIL
                     GIKDKETFPLIFYRDNCADMAITKDDFSEAYIASSRCLAITGTHLSNPKTREAVLTAL
                     EYAGRNEVKRALDIDYRPVLWGLTSLGDGETRYIDSAEVTKSLQEVLHHFDLIVGTEE
                     EFHIAGGSTDTLTALKNVRKVSNAVLVCKRGALGCSVFEGELPNDLDGGLNVYGVRVE
                     VLNVLGAGDAFMSGLLRGYLNNEGWEQACHYANACGALVVSRHGCSPAMPTKRELDDY
                     LTRAESVPRPDLDERLNHLHRVTTRKKQWENLCIFAFDHRKQLVDMANKVGADLARIP
                     KLKQLLLKAMEQTATQSGLPQNQAGILADTTFGQDVLNEITGKDYWIARPIEEPSSRP
                     LELEYGDLGSQLASFPRDHVIKCLAFYHPNDESGLKARQDRKLKEVYQACCRTGHELL
                     LEIILPADMPQSEAFYNQAIAHFYQLGIKPEWWKLPDVSRAQWQAISATIEANDPDCR
                     GILILGLDAPLEVFKDTFEQAKGCEKVKGFAVGRTIFGEPSEKWLAGELDDNTLISAV
                     SEKYRTLIDLWQQR"
     gene            2231..3571
                     /locus_tag="PXD71_000355"
     CDS             2231..3571
                     /locus_tag="PXD71_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HDL6290516.1"
                     /translation="MSCNTSPKRFHASWESIKQHQVPEWYEDAKFGIFIHWGIYSVPA
                     FAPPTCQLGEIDIDEQWFCNNPYAEWYFNSINVKKGPTYEHHIKTYGESFAYNDFLPL
                     WKAEKWQPAEWAKLFAKAGAKYVVLTTKHHDGFCLYPSKYTDFNCMQAGPKRDLMGEL
                     TENVREQGLKMGAYYSGIIDWTYSSAPIFTESQNFSNACPTYEYADYAYKQVVELIDR
                     YKPDVLWNDIGWPKVGEHMLPHLFAHYYNSVQEGVVDDRWNKLWCDFTSKEYKHGVAS
                     RDKKWEMCRGMGLSFGYNQVEDESHLISVKDLISLLVETVADNGNLLLNIGPKADGTI
                     PQEQVERLLALGSWLEINGEGIYKSRCSQHQTEQIGDVTYRYTKVGADLYLYIDGLTE
                     AQISLPLSVYGELQALNTELKFTIENTAQGRVLHIQNYQPDWYVLGFKIADGEK"
     gene            3574..4914
                     /locus_tag="PXD71_000356"
     CDS             3574..4914
                     /locus_tag="PXD71_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL6290517.1"
                     /translation="MSVSQRFIDFMNAHVAPVARRMENQPHISAIRDGFIVVLPFLIV
                     GSFIMILLIPPFDENTQNAFGQAWWRFANWASPYGWNFFQMSFNAISLFTSASIAYNL
                     AKAYKREPLPAAFLSVMAFLLVAAPVKDGMMDIKFFGGIGLFSAIFIAIYSVEMTRLL
                     EFLKIKIRLPKEVPHAVAESLNIVIPILAILLTLYPFAIWVESATGRNIPQLIMDFMA
                     PLIAVSDSLGAICLFVIATHLLWFLGINGSLVLMQLWTPFLLQNMAANLAAFQAGEPL
                     PFIITNSFWDFYIVHGASGGVIALAFLLVRSKSAHLRSIGKIGLVPSFFSIGEPIVYG
                     VPMVVNPLFFIPLIFAPLANAVIAYLILDFNLIHRVYLMAPWTTPAPIGAYLVSAGDI
                     WAPILSIALIILDIVIYYPFFKMYEKVCVEKEKAQNLDEKAQQELLEKARMAAQ"
     gene            5052..5876
                     /locus_tag="PXD71_000357"
     CDS             5052..5876
                     /locus_tag="PXD71_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL6290518.1"
                     /translation="MNGPTQLAHFQEQIRKEYDGLSKRLKQVAQYVLDNGNSVVFDTV
                     STIAERADVPPSTLIRFANAFGFSGFNEIKQLYRQDMMEGTSRYTERVQLFRQIEPLD
                     EQNSELNHILDVFVQGNTQALQQLSTQVSEEQLAQTVDILHNAKRIFIVGLKRSFSIA
                     NYLNYALHHLDYDVFMIDGSGGMFDEQLSRIREGDAVIAVSFSPYANETLNVVTATAR
                     PGVKHIAITDSQLSPLLSFSDVSFIIKEAQVSGFRSQCATMTLAQTIAVSLALKKA"
     gene            complement(5920..6162)
                     /locus_tag="PXD71_000358"
     CDS             complement(5920..6162)
                     /locus_tag="PXD71_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL6290519.1"
                     /translation="MSDKSIILEIINALGNKENIKQVEACLTRLRVVLHNNGLLNKSA
                     LKKLGAVDVIKVGDTQQIIFGAKAGQYRDEINALIG"
     gene            6427..6675
                     /gene="rpsP"
                     /locus_tag="PXD71_000359"
     CDS             6427..6675
                     /gene="rpsP"
                     /locus_tag="PXD71_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL6290520.1"
                     /translation="MVTIRLTRGGAKKRPFYQIVVVDSRSPRDGRFIERIGFFNPLAA
                     GQAERLRLDVAKVDAWVAKGADLSDRVASLVKEARKAA"
     gene            6708..7235
                     /gene="rimM"
                     /locus_tag="PXD71_000360"
     CDS             6708..7235
                     /gene="rimM"
                     /locus_tag="PXD71_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL6290521.1"
                     /translation="MSEQKIEVVGKLGSTYGIRGWLRLYSSTEQTESIFDYQPWFLKI
                     KGQWQQVELESWRYHSNDLIVKLKGTEDREAAQLLTNAEIGVDLSVFPELEEGDYYWH
                     DLIGCSVVNLEGYTMGTVAEMMETGSNDVLVVRNNGKDAFGKQERLIPFLYEQVVKRV
                     DLATKTITVEWDAGF"
     gene            7280..8035
                     /gene="trmD"
                     /locus_tag="PXD71_000361"
     CDS             7280..8035
                     /gene="trmD"
                     /locus_tag="PXD71_000361"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL6290522.1"
                     /translation="MWIGIISLFPEMFKAITDFGVTGRAVKQNLLQVECWNPRDFTFD
                     KHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKKAALAVEGGEAKVIYLSPQGRKLDQNG
                     VQTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
                     PGVLGKQASALEDSFAEGLLDCPHYTRPEVLDGMPVPEVLMSGHHEQIRKWRLEQSLE
                     RTWLRRPELLDSLALTDEQRVLLAKIKKQHKIS"
     gene            8062..8412
                     /gene="rplS"
                     /locus_tag="PXD71_000362"
     CDS             8062..8412
                     /gene="rplS"
                     /locus_tag="PXD71_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL6290523.1"
                     /translation="MSNIIKQLEQEQLKQDLPSFRPGDTLEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTLRKVSNGVGVERVFQTHSPVIDSISVKRKGAVRKAKLYYLRE
                     RSGKSARIKERLGD"
     gene            8568..8924
                     /locus_tag="PXD71_000363"
     CDS             8568..8924
                     /locus_tag="PXD71_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290524.1"
                     /translation="MKQLSIRLPDDEFTFIKTFASAHKLTLQNFVMSAIQAKVAEMSA
                     KNKISEESNIHQPLSKDEKIMLNEAKVFYSARSHLKAENVILPSDFMEQIKSGCGAEK
                     MAEMMGYTKEDWESMK"
     gene            8926..9249
                     /locus_tag="PXD71_000364"
     CDS             8926..9249
                     /locus_tag="PXD71_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290525.1"
                     /translation="MGQITFSNIFTKVIFENVLSASTKEKIKQMLFQSVVALPPLHSE
                     RKMILQLKKQKITIFYQVIEEQSVQILAYQFGGTDKLTGVSKAHFKTLDAIFQMTDEE
                     KEGKF"
     gene            complement(9259..9591)
                     /locus_tag="PXD71_000365"
     CDS             complement(9259..9591)
                     /locus_tag="PXD71_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL6290526.1"
                     /translation="MQIEQYQQWVRQFYQQQGWYERSPFMRVTYLAEEIGEVACAVRA
                     IEIGRERPDETEASQQQKRDNLIEELGDVMDNLFVLADKYGISMTEVLERHQAKFEKR
                     YLQKNMEK"
     gene            complement(9684..10880)
                     /locus_tag="PXD71_000366"
     CDS             complement(9684..10880)
                     /locus_tag="PXD71_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL6290527.1"
                     /translation="MNIPFCLPENISPETFLREYWQKRPLLIRNGLPQIVGLFEPEDI
                     LELALEEEVTARLIQCENEKWSVKSSPLVEEDLEELPVQWSVMVQNLEQWSPELGELW
                     QAFGFIPQWQRDDIMVSCSPLNGTVGKHYDEYDVFLVQGYGQRRWQLGKWCDPSTEFK
                     PNQPIRIFDDMGELVMDEVMNPGDVLYVPSRMAHYGVSESEQGLTFSFGLRYPNATDL
                     LENLCKTIEQQADNINSSEFHLPFRLTPNEQPNALLDPKMVKMFKHQLIDLLQNSEQF
                     DELLTHSVATAASSRRYELLQTDNEYYPDEVQEILEAGGWLQQDANVKILYTENPQRV
                     YVNGEWIDELNEAEMALLIRIANGDSISWNKLASKVQDQAELELMLDTICDWLDSGWI
                     ILSEAE"
     gene            complement(11029..12372)
                     /gene="accC"
                     /locus_tag="PXD71_000367"
     CDS             complement(11029..12372)
                     /gene="accC"
                     /locus_tag="PXD71_000367"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL6290528.1"
                     /translation="MLEKVVIANRGEIALRILRACKELGIKTVAVHSTADRELKHVKL
                     ADETICIGPAPSVKSYLNIPMIIAAAEVTNADAIHPGYGFLSENANFAEQVEKSGFIF
                     IGPTADVIRLMGDKVSAINAMKKAGVPCVPGSDGPVGNDPKKNKEIAKRIGYPVIIKA
                     SGGGGGRGMRVVHHEKDLEESIAMTKAEAKAAFNNDMVYMEKYLENPRHIEIQVLADT
                     HGNAVYLAERDCSMQRRHQKVVEEAPAQGITPELRQFIGERCANACREIGYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHPVTEMITGVDLVKEQLRVAAGLPLSITQDDIKVKGHA
                     IECRINAEDPNTFLPSPGKITRLHVPGGLGVRWDSHIYSGYSVPPHYDSMIGKLITFA
                     ENRDIAIRRMENALSETIVEGIKTNIPLHTQILADKEFRKGGTNIHYLEKKLGLK"
     gene            complement(12444..12911)
                     /locus_tag="PXD71_000368"
     CDS             complement(12444..12911)
                     /locus_tag="PXD71_000368"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL6290529.1"
                     /translation="MDIRKIKKLIELVEESGITELEVSEEEGTVRISRAAPAVAPAAV
                     QYAAAPVVAPAAPQVASTVPVAAPAEAPKAAETSGHAVLSPMVGTFYRSPSPDAKPFV
                     EVGQTVKIGDTLCIVEAMKMMNRIESDKAGVVKEILVNDGEPVEFDQKLFIIE"
     gene            complement(13164..13946)
                     /locus_tag="PXD71_000369"
     CDS             complement(13164..13946)
                     /locus_tag="PXD71_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL6290530.1"
                     /translation="MAVKNEQFIVICNGVFRLFGNIVKILSYPFHALFPKKRFTIPEY
                     SPAKIQSSIPSKITKTIWQTNYSNKVTLPMYANYLFNRLMSLSYDYRYVSTEEREAYI
                     KANADERTFNAYSKLTDGAAQADFWRIFTLLREGGIYIDIDGHLVYPLSKIIGENDSE
                     VLIKRRDKYTNFFLASEAGNWILKDTLELIISNIENRRIEGGVFVMTGPDTLNNAIGD
                     KEVNFRRDKVTCAQGTFTNEYFQYIDKPGSKWNYKKNEDLLK"
     gene            complement(13956..14393)
                     /gene="aroQ"
                     /locus_tag="PXD71_000370"
     CDS             complement(13956..14393)
                     /gene="aroQ"
                     /locus_tag="PXD71_000370"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HDL6290531.1"
                     /translation="MKKILLLNGPNLNMLGKREPQIYGSQTLADIENHLKKLAAAQDV
                     ELESFQANGEEPIINRIHQAFQHTDFIIINPGAFTHTSVAIRDALLSVSIPFVEVHLS
                     NVHSREPFRHHSYLSDIAKGVICGLGAKGYEYALDFAISHLKA"
     gene            complement(14479..14847)
                     /locus_tag="PXD71_000371"
     CDS             complement(14479..14847)
                     /locus_tag="PXD71_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6290532.1"
                     /translation="MAFTIRKMVEQDTHQVFPLMQKLAVFEHYIDSFAITPEVVMESG
                     FRKSPPDFYCLVAENASNIIGILVYYFLPYTAQNRPAIYMKELYVDETARNQKVGEAL
                     MQALKNEAKAHHCKTIKSLE"
     gene            complement(14907..15731)
                     /gene="dapD"
                     /locus_tag="PXD71_000372"
     CDS             complement(14907..15731)
                     /gene="dapD"
                     /locus_tag="PXD71_000372"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDL6290533.1"
                     /translation="MSLQAIIEAAFERRAEITPKTVDAETKAAVEEVIAGLDNGSLRV
                     AEKKDGEWVVNQWVKKAVLLSFRIADNEIIDGAETKFYDKVPTKYGNYTEEQFKADGI
                     RAVPGAVVRKGSHIEKNVVLMPSFVNIGAYVGEGTMVDTWVTVGSCAQIGKNVHLSGG
                     VGIGGVLEPLQANPTIIGDNCFIGARSEIVEGVIVEDNCVISMGVFIGQSTKIYDRET
                     GEVHYGRVPAGSVVVSGSLPSKDGSHNLYCAVIVKKVDEKTLSKVGLNDLLRSIDE"
     gene            15879..16541
                     /locus_tag="PXD71_000373"
     CDS             15879..16541
                     /locus_tag="PXD71_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL6290534.1"
                     /translation="MENFANLLTGKTREHLVPLPNALSDKHYLQPQVVEAFLTLQHAA
                     KQAGFNLQPASTFRDFERQKGIWNAKFRGERKVHDDNGVAIDMAALSDLEKCKAMLRW
                     SAVPGASRHHWGTEIDIFDPDLLPENTPLLLEPWEYLEGGYFADLTKWLHVNAETFGF
                     YFPFDGKHSRVGFEPWHISYRPISNEYEQRFNDEILKAAWQDEEVAGKECLLEHFEEL
                     LK"
     gene            16538..17290
                     /locus_tag="PXD71_000374"
     CDS             16538..17290
                     /locus_tag="PXD71_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6290535.1"
                     /translation="MIQLINETQQHDKFRQICDKWQLVEDKSSPLALVLTEERLELRK
                     LDEPKLGAIAVNFVDGTMAHRRKFGGGRGEAVAKAVGIKGDYLPTVIDATAGLGRDAF
                     VLAAVGCKVLLVERNPIVAALLEDGLNRAYQDVEIGSFMQERMILADVRDISQLDPQS
                     QMAEVVYLDPMYPHKQKSALVKKEMRVFQHLVGADLDSDSFFLPAKGLAKKRVVVKRP
                     DYAPFLAEQKPDFSHTTKNHRFDVYLSHLKND"
     gene            17381..18217
                     /gene="ftsN"
                     /locus_tag="PXD71_000375"
     CDS             17381..18217
                     /gene="ftsN"
                     /locus_tag="PXD71_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDL6290536.1"
                     /translation="MPQRDYVARSNEKKSKKNQKESNVKKILMRIIGVILFFGIGLWF
                     LKSSAPVPTVPSAPQPANSQTKSELPSRPEETWSYIRDLETRTVITDNSQTSQERLAQ
                     LTAEQKQQIEERRKQEEARLAAEQAQQKPATDVSATELAPKTEEVKSLTEEELAAKKA
                     QEQALAQKQAEEKRRQAELKKQQEAKKEQPAKIVASAATLADKAANQAGKFGLQCGAF
                     KNKAQAENMQARLAMAGFNARIASSADWNRVFVGPIGDRAATSRAQSNARSVAECVIV
                     AM"
     gene            complement(18259..19440)
                     /locus_tag="PXD71_000376"
     CDS             complement(18259..19440)
                     /locus_tag="PXD71_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL6290537.1"
                     /translation="MKTELDMIVIGGGMIGAATALGLAKQGLRLALLEKNPLPTFNPD
                     SDYDLRISAISMGSIQLLEKLGVWEQIVAMRSTPYTQLETWEIDGFNTLFSAADLGLE
                     KLGDMVENNVIQIALWEQLKQYPNCLQAVGFEQIFANKAGEYWEVTVDGNTFAAPLLI
                     AADGANSQVRQWAGIGLTAWQYRQHCLLATVTTEKPAPSGTWQQFYPTGPRAFLPLSG
                     NHGCVVWYDSPQKIAELKMLPAEKLGVAIEQHFPSRLNAELGNIQVQASGAFPLTRQH
                     AQHYVQNGVVLVGDAAHTINPLAGQGVNLGFKDVKTLLEVIEQAVKKGKNFASEQVLQ
                     RYEQKRKPDNLLMQTGMDVFYKAFKTDLLPLKIARNVGLVLADKVSPIKKQALKYAIG
                     I"
     gene            19547..19885
                     /locus_tag="PXD71_000377"
     CDS             19547..19885
                     /locus_tag="PXD71_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290538.1"
                     /translation="MSCKTHANHEHEHGEGCGHTAIKHNDHIDYLHDGHLHHNKHGDH
                     YDEHVLEVNETNPADCNAAHCCGDHVHGEVCGHEAVPHGDHIDYIVNGRLHHQHGDHC
                     DDHGPVEIVK"
     gene            20017..20886
                     /locus_tag="PXD71_000378"
     CDS             20017..20886
                     /locus_tag="PXD71_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDL6290539.1"
                     /translation="MTLAIVYSRASIGVEAPLVTIEVHISNGQPKLTIVGLPEATVKE
                     AGDRVRSALLNANFIYPPQRITINLAPADLPKEGGRFDLPIAIGILAASGQIDSDLLT
                     QFEFLGELALTGHLRGVHGTIPAVISADKAKRQMILAKQNANEASLVSNATTYFAGSL
                     LEVVNMLNKRDKLPICQHISQHSAEIRPLVSRDLTDIIGQQHAKRALMIAAAGQHNLL
                     FLGPPGTGKTMLASRLADLLPEMTDEEAIETASVTSLVQNELNFQNWKQRPFRSPHHS
                     ASMVALVGGGCEN"
     gene            20978..22789
                     /locus_tag="PXD71_000379"
     CDS             20978..22789
                     /locus_tag="PXD71_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290540.1"
                     /translation="MFYLNNDNFKREPYRGYCPSHLEKLFTKPHLDIRGNGYYYPDNE
                     YVVAWYESDFIFKSRKAQAHAKVKLVNLTCPKYTGKNSAEYLSVLYISVPLNYLHYFT
                     IGSIWKGGVAKEQFALEEFYITVEAENQDGKRENLSIVSFGESEENGLEKPFDYTIYT
                     IPTEYHNYGDDLNRLLSISYQNQKFIIHPLHIFMMHYGYSTDIKRILATYPLDEVRER
                     LFIDKVVENFDVERYVVLPKYFVKKDAIFLYHFKYDEETTGTRVKKLVSQFRLNIHNQ
                     THPIEIDFWHTQKVELKIRGVRLGNAVLCAEIIGLNQPEGEDITLVLSQSKREKSGNK
                     TEEQNNEVNNVPITRVYTREPELDELPLTDNSPDNRTVEYNKRQFELLGRQRQIRALK
                     KAREESNQKMKALTPDEIDSLGVGESDGRNGKVGLAFCFLDDTPVGKSSKLYKLWQHA
                     KAVAEWNHGEAHWYTPNLGFRNDDELLPVSLETNKCFDYPEIAIIIRLQIYGETFFLI
                     DFSMKQRDISIRGLGYKPDPNEDFLYESDMSKSELSELLSAVHHHEHLPKEYIEKKNK
                     EKTKLTTFNHSEAESSNWAYNAVQKLTSRAITKPTYF"
     gene            22798..25578
                     /locus_tag="PXD71_000380"
     CDS             22798..25578
                     /locus_tag="PXD71_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDL6290541.1"
                     /translation="MNAEQRVIVTPTFWGKFFGKIKSVELQQNKVIVTDKKNNITEHD
                     LGKTFDFPAIQKSFFGTKLFFKDDSTEVVLSKLAKKQTDSLLLEIEKVVASNIKVKVK
                     EGFQHFANLAENQYLRDSDIPTLNDRVRLSVLSYGDNKEHFQKYFDESLVKKIQYISS
                     LFPVNAINAQTIRREYEQHVLNHRKHFFDVIESNPLTEQQRLSVIRNNDINMVLAAAG
                     TGKTSVMVAKALDLIDSGACKSEDILILAYNRDAATELSERLQERAREANLDLENSPS
                     ISTFHALGRRIIKECGKSVHLSKFAEDPMKLDRWFNKWLEDYIKEDPDNLAKFIQLAY
                     QPIDPFQFKTKDEYDRYVNDNDYRTLQGERVRGYQELLIANWLFSHSVEYEYEPRYVS
                     KRRIEIGFDYKPDFSLGDGVYLEHFGIDRQGRTRADINAQEYNANIQRKRELHAEHNT
                     TLLETYHYNWVENTLYKRLEQLMNEQFIPLKPKSQQEILDALNESGIFKENKNRYLKC
                     LQAIRTERLDYQQILKRLTDAKIVYAKEYATLLMRIHDAYVKELRSANEIDFDDMILL
                     ATQLVKTGEFKPKWKHILVDEFQDISMARLELLKEIYTKGPRPIWTVVGDDWQSIYRF
                     SGGKLEVTTRFNDMVGSHTLSKLEKTYRYNNSIADTAGQFIMQNPEQYQKNVVTHTKV
                     ADSCVHLYDSHVVKDEKSEANISLKASAILKLIRQKAPEATVAILARYRYLLEDAKSV
                     IKDKNVKFWTFHGSKGLEADYCILLGFFQGKVGFPNQNKEEAVVEALLPMLDSFPHSE
                     ERRLFYVALTRAKKESYLIADANAPSEFINELLSPKYKIDIVSKRFQERYRTIFKCNE
                     CSTGYFVMKQGKFGQFYSCTSGEVCRSSPRICEKCGRPMKLREGKFGQFWGCSGYGIK
                     GDQCTNTRKYFM"
     gene            25600..>26269
                     /locus_tag="PXD71_000381"
     CDS             25600..>26269
                     /locus_tag="PXD71_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL6290542.1"
                     /translation="MDLFESKKQLIELIKKYDSDKEIYSSSSYNESQLRTDFLDPFFG
                     LLGWDITNSKNKLTSEREVIVEEPLKEKVSENVKKPDYTFRFNSQRKFFVEAKKPSVK
                     IKTDPLPAHQVRRYGFTAKLSISVLSNFEYLAIYDCSEIVDVQQDALYARVRLYHYTE
                     YVKFFDEIMNLLGRESVYSGHFDNEWREISQKIENSKQTVDELFFEQINKWRHVLGSE
                     LLKIN"
BASE COUNT         7837 a         5334 c         5628 g         7470 t
ORIGIN      
        1 aatttacttg ttgtaaggag cggttatggc aacagttaat catcaagatc gtccattgga
       61 tcttatctgt ttagggcgtg tggcggtgga cttttatggg caacaaatcg gttcccgttt
      121 ggaagagatg accacatttt caaaatacct cggcggctcg tcaggcaatg tggcttacgg
      181 tacggcggtg caaggtttac gttcttcaat gctggcaagg gtcggtgacg aacatatggg
      241 gcgttttcta cgtgaagagc tacaaagcgt aggcgtggac accagccatt taattaccga
      301 taaagaacgc ttaactgcac tggttattct cggcattaaa gataaagaga cttttccgct
      361 gattttctat cgtgataact gtgcggatat ggcaatcacc aaagacgatt ttagcgaagc
      421 ctatattgct tccagccgtt gcttagcaat taccggcacg cacctgtcaa accctaaaac
      481 ccgtgaagct gtgctgaccg cactcgaata tgcgggtaga aatgaggtaa aacgagcgtt
      541 agatattgat tatcgccctg tgttatgggg attaacctca ctcggtgatg gcgaaacccg
      601 ttatatagac agtgccgaag tcaccaaatc gctacaagaa gtattgcacc atttcgatct
      661 gattgtcggc acagaggaag aatttcatat cgcaggcggt tcgaccgata ctctcactgc
      721 actcaaaaat gtgcgtaaag tctctaatgc cgtattagtg tgcaaacgtg gggcattagg
      781 gtgttcagta tttgaaggcg agctgccgaa tgatttagac ggtggcttga atgtgtacgg
      841 cgtgcgtgta gaagtgctga atgtgctggg tgcaggcgat gcttttatgt cgggcttact
      901 gcgtggctac ttaaataacg aaggttggga acaagcgtgc cactatgcca atgcgtgcgg
      961 agcgttagtg gtatctcgtc acggctgttc accggcaatg ccgaccaaac gtgaacttga
     1021 cgattattta acccgtgcag agagtgtacc acgtccggat ttagatgagc gtttaaacca
     1081 cttacaccgt gtcaccaccc gcaaaaaaca gtgggaaaat ctctgtattt ttgcttttga
     1141 tcaccgcaaa cagttggtgg atatggcaaa taaagtcggt gcagatcttg ctcgcatacc
     1201 gaaactgaaa cagctgctat taaaagcgat ggaacaaacc gcaactcaat ccggcttacc
     1261 acaaaatcaa gcagggatcc tagccgatac cactttcggg caagatgtac ttaatgagat
     1321 taccggcaaa gattactgga ttgcccgccc gattgaagag ccgtcttccc gcccgctgga
     1381 attagagtat ggagatttag gctctcagct agcatcattc ccacgcgatc acgtcatcaa
     1441 atgcttggca ttttaccacc cgaatgatga aagcggttta aaagcccgcc aagatcgcaa
     1501 gttaaaagag gtgtatcaag cctgttgccg taccggtcac gagctattgt tggaaattat
     1561 tctgcctgct gatatgccac agagtgaggc tttctataac caagcgatag cacatttcta
     1621 ccaacttggc attaagccgg aatggtggaa attgccggat gtttctcgcg cacaatggca
     1681 agcgatttcc gccaccattg aggcaaacga tccggactgt cgtggtattt taattttagg
     1741 tttagatgcc ccgttagagg tgtttaaaga cacctttgaa caggcaaaag gctgtgagaa
     1801 agtgaaaggc tttgcagtcg gccgcaccat tttcggtgaa ccgtccgaaa aatggcttgc
     1861 cggtgaattg gacgacaata ccttgatttc tgcggtatcg gagaaatatc gcactttaat
     1921 cgatctctgg caacaacgtt aatcatattg ctttaagtga aaacccctta gcttatgata
     1981 ttaagcaagg ggttttgttt tgcaaaaaat ccggctaaac agaccgcttg ctagccgtaa
     2041 aaatgcccga attttcctaa aaaagcgaat atcgggcatt cctcaatctg cttatcacaa
     2101 aataagtgtg atgaagttca catttcatac ataaaccccc ccctttttat cgaaacaaat
     2161 tgttttattt ttatcaaaat ggaaaatgta tttcagttta gttctattta gattcaatag
     2221 gagtctgtgt atgagttgca acacttcacc aaaacgtttc cacgccagtt gggagtcgat
     2281 caaacagcac caagtaccag agtggtatga agatgctaag tttgggattt ttattcactg
     2341 ggggatttac tccgtacccg cctttgcccc accgacctgt cagttaggtg aaattgatat
     2401 tgatgaacag tggttctgta ataaccctta tgcagaatgg tattttaatt ccatcaatgt
     2461 caaaaaaggc ccgacctacg aacatcatat caaaacttat ggcgaatcct ttgcctataa
     2521 cgacttcctg ccactatgga aagcggaaaa atggcaacca gcagaatggg cgaaactgtt
     2581 tgccaaagct ggcgccaaat atgtggtatt aaccaccaaa catcacgatg gtttctgtct
     2641 ctatccaagt aaatataccg atttcaactg tatgcaagcc ggaccgaagc gggatttaat
     2701 gggcgagctg actgaaaatg tgcgggagca aggcttaaaa atgggcgctt actactcggg
     2761 cattatcgat tggacttatt cctctgcccc gattttcacc gaaagtcaaa acttctcaaa
     2821 cgcttgcccg acttacgaat acgctgacta cgcctataaa caagtggtag agctgatcga
     2881 ccgctataaa ccagatgtat tgtggaatga tattggctgg ccaaaagtgg gcgagcatat
     2941 gttaccacac ctatttgctc attattacaa cagcgttcaa gagggcgtag tggacgaccg
     3001 ctggaacaaa ctatggtgcg atttcacctc caaagaatac aaacacggcg tggcttcccg
     3061 tgataagaaa tgggaaatgt gccgtggtat gggcttatcg ttcggctata accaggtaga
     3121 agacgaaagc catttgattt cggtgaaaga cttgatttcc ctattggtag aaaccgtagc
     3181 cgacaacggc aatttattgc tgaatatcgg cccgaaagca gatggcacta ttccgcaaga
     3241 gcaagttgaa cgcttattag cactggggag ctggctggaa ataaatggag agggaatcta
     3301 taaatcccgt tgtagccaac accaaaccga gcaaatcggt gatgtaacct atcgttatac
     3361 caaagttggg gcggatctct atctttacat tgatggctta accgaagccc aaatcagctt
     3421 gccactgtca gtttatggtg agctacaagc actcaatacc gagctgaaat ttaccattga
     3481 aaacaccgca caaggcagag tgttacatat tcaaaactac cagccggatt ggtatgtatt
     3541 aggctttaaa attgcagatg gggagaaata ggtatgtccg tttcacaacg ttttattgac
     3601 tttatgaatg cccacgttgc tccagtagca cgtcgaatgg aaaatcaacc gcatatttcc
     3661 gctattcgag acggttttat tgtagtgtta ccattcttaa ttgtcggcag ctttattatg
     3721 attttgctaa ttccgccatt tgatgaaaat acccaaaatg ctttcgggca agcttggtgg
     3781 cgttttgcga attgggcaag cccttacggt tggaatttct tccaaatgtc gtttaatgcg
     3841 atttcattat ttacctctgc cagtatcgcc tacaaccttg ccaaagccta taaaagagag
     3901 cccctgccag cggcatttct ctcggtaatg gcatttttgt tagtggctgc accggtcaaa
     3961 gacggaatga tggatattaa attctttggc ggtattgggc tattctctgc gatttttatt
     4021 gccatttatt cggtagaaat gacccgcttg ttggagttct taaaaattaa aatccgctta
     4081 ccgaaagaag tgccgcacgc tgtagcagaa tctctgaata tcgtgattcc aattttagcg
     4141 attttactga ctctctatcc gtttgccatt tgggtggaaa gtgccacagg tcgtaatatt
     4201 ccacaactaa ttatggactt tatggcgcca ttgattgctg tgagtgactc gctaggggca
     4261 atttgcttgt tcgttattgc tacccatttg ctctggttct taggcattaa cggctcattg
     4321 gtactaatgc aactttggac accattccta ctgcaaaata tggcggcaaa cttagcagca
     4381 ttccaagctg gtgaaccgct cccctttatc atcaccaatt ccttctggga tttctatatc
     4441 gtacacgggg caagtggtgg tgtaatagcc ttggcattcc tgctggtacg cagtaaatcg
     4501 gcacacttac gctctatcgg taaaatcggc ttggttccgt ccttcttctc tatcggtgaa
     4561 ccaattgtgt atggtgtgcc aatggtggtt aatccgctgt tctttatccc gctgattttc
     4621 gcaccactag cgaatgcggt tattgcctac ctgattttag attttaacct tattcaccgt
     4681 gtttacttga tggcaccgtg gacaacaccg gcaccgattg gggcatatct ggtttctgcc
     4741 ggtgatattt gggcaccaat tttaagtatt gcactgatta ttttggatat tgtgatctac
     4801 tatccattct tcaaaatgta tgaaaaagtc tgcgtcgaga aagaaaaagc acaaaatcta
     4861 gatgaaaaag cacaacagga gttgctagaa aaagcacgaa tggcagcaca ataacactta
     4921 tttctagcgt aattgagagg ggctaatcga aagattagcc cttatttttg caaaaaattg
     4981 acaaaattta tccgcttgta aacaagaaaa gcgtataatg aaataaaaaa ttcaagcaga
     5041 gataatttga aatgaatgga cctactcaat tagcacattt tcaagaacaa atccgaaaag
     5101 agtatgacgg actaagtaaa cgtttaaaac aagtggcaca gtatgtgctg gataatggca
     5161 acagcgtggt gtttgatacg gtttctacca ttgctgaacg agctgatgtt cccccttcaa
     5221 ccttaatccg ttttgcgaat gcgtttggat ttagcggttt taatgaaatt aaacagcttt
     5281 atcgccaaga tatgatggaa ggcacatcac gttacaccga acgtgtgcaa ctttttcgtc
     5341 aaattgaacc gcttgatgag caaaacagtg agctcaatca tattttagat gtgttcgtac
     5401 agggtaatac gcaggcactg caacagttat cgacacaagt cagcgaagag cagttagcac
     5461 aaacggtaga tattttacac aatgccaaac gcatttttat tgtgggttta aaacgctcat
     5521 tcagcattgc gaactaccta aactatgcgt tgcaccattt ggactatgat gtctttatga
     5581 tcgatggttc gggcggtatg tttgacgagc aattaagccg aatccgtgaa ggtgatgcgg
     5641 tgattgccgt gagcttttcc ccgtatgcga atgaaacact caacgtggtg acagcaactg
     5701 ccagaccggg ggtaaaacat attgcgatca ctgatagcca acttagccca ctcctttcat
     5761 ttagcgatgt gtcgtttatt attaaagaag cacaggtgag tggttttcga tcccagtgtg
     5821 ccacaatgac gctcgcacaa acgattgccg tttcactggc gttgaaaaaa gcctagatta
     5881 agaaagtgaa agacctgcgt aaatacaggt ctttgtctat taaccgatta aagcgttaat
     5941 ttcatcacga tactgcccgg cttttgcacc aaaaataatt tgttgcgtat caccgacttt
     6001 aatgacatca accgccccaa gctttttcag tgccgactta tttaataaac cgttgttatg
     6061 taacaccaca cgtaaacggg ttaaacaagc ttcaacttgc ttgatattct ctttgtttcc
     6121 taaagcatta ataatttcta gaatgataga tttatcagac atatttcctc catacctatt
     6181 tatatttaaa actcttatta tgatatttta aacattttct gatggttaga acaattttta
     6241 ttagtaattt gtataaaaaa acgctaaatg tatctttctt taaaagagtc atatcaatga
     6301 atctcccttg tctttccgca aaaaatctgt ataatttgac cgcttgtttt ctcttttgaa
     6361 acaagttcgt taccctcacc tcgaacgagg gtttattttt tactatcaac atttagagga
     6421 aaggttatgg taaccattcg tttaactcgt ggcggagcta aaaaacgccc attttaccaa
     6481 atcgtggtag tggacagccg ttcacctcgt gatggtcgct ttatcgagcg tatcggtttc
     6541 ttcaacccat tagcagcagg tcaagctgag cgtttacgct tagatgtggc taaagtagat
     6601 gcttgggttg ctaaaggtgc tgatttatca gaccgtgttg cttctttagt aaaagaagct
     6661 cgcaaagcag cttaattttt aagccaaaat taaataagta ggtgagtatg agcgaacaaa
     6721 agatcgaagt tgtcggaaaa ttaggttcaa cctatggtat ccgtggttgg ttgcgtctct
     6781 attcatcgac agaacaaact gagagcattt tcgactacca gccgtggttt ttaaaaatta
     6841 aaggtcagtg gcagcaggtc gaattagaaa gctggcgtta tcatagcaat gatttgattg
     6901 ttaaattgaa aggcactgaa gaccgtgaag ccgcacagtt attaaccaac gctgaaattg
     6961 gcgtggattt gtctgtgttt cctgaattgg aagagggaga ttactattgg cacgatttga
     7021 tcggctgttc ggtagtaaac ttagagggct acacaatggg aacagttgcc gaaatgatgg
     7081 aaacaggttc aaatgatgtg cttgtggtgc gtaataacgg caaagatgct ttcggtaaac
     7141 aagaacgatt aattccgttt ttgtatgaac aagtagttaa aagagtagat ctcgccacca
     7201 aaaccattac ggtggagtgg gacgctggtt tctaacctca ggtatgcggt gagctttgga
     7261 aaacatttaa ggcgttaaaa tgtggattgg tattatttca ctgtttcctg aaatgtttaa
     7321 agcaattacc gatttcgggg tgacaggacg tgcagttaaa caaaatctac tgcaagttga
     7381 atgttggaat cctcgtgatt ttacattcga taaacataaa actgtagatg atcgccctta
     7441 tggcggtggt ccgggaatgt tgatgatggt gcaacctttg cgtgatgcga ttcacgcagc
     7501 gaaaaaggcg gcattagcag tagaaggcgg tgaagctaaa gtgatttatc tttcgccgca
     7561 aggacgtaaa ctcgatcaaa acggtgtgca aacactggct tcaaatcaga aactgatttt
     7621 aatttgtgga cgatatgaag gcgtggacga gcgattgatt caaacagaag ttgatgaaga
     7681 atggtcaatc ggtgattacg ttctcacagg gggggaatta cctgcaatga cattaattga
     7741 tgcagttgca aggtttgttc ccggggtatt aggcaaacag gcttccgcgt tagaggattc
     7801 tttcgcagag ggattattag attgcccaca ttatacccgc cctgaagtat tagacggtat
     7861 gcctgttccg gaagtgctga tgtctggaca ccatgaacaa attcgtaaat ggcggttgga
     7921 acaatcgctg gaacgaacgt ggttaagacg ccctgagcta ttggatagcc tagctctgac
     7981 tgacgaacag cgtgtgttgt tagctaaaat taaaaaacag cacaaaatca gttaattcta
     8041 ggattataga aggtttttaa aatgagtaac atcatcaaac aacttgaaca agaacagtta
     8101 aaacaagact tacctagctt ccgtccgggt gacacattag aagttaaggt atgggtagta
     8161 gaaggtagta aaaaacgttt gcaagcgttc gaaggcgtgg ttattgcaat tcgtaaccgt
     8221 ggcttgcact ctgcattcac tctacgtaaa gtttcaaatg gcgtaggcgt tgagcgtgta
     8281 ttccaaactc actcacctgt aattgacagc atttctgtta aacgtaaagg tgcggtacgt
     8341 aaagctaaac tttactactt acgtgagcgt tctggtaaat ctgcacgtat caaagagcgt
     8401 cttggcgact aattgctaca aaacagtgaa atgcacaaaa gccttgagga aactcaaggc
     8461 ttttgctttt tgtacttaca agcagttatt tttcctcaat gatttacaaa tcttgctttc
     8521 ttttttgtta taatgttaac aagataacat taatgatagg tgtcgatatg aaacaattaa
     8581 gtattagatt gccagacgat gaatttactt ttattaaaac atttgcttct gctcataaac
     8641 tgactttaca aaattttgtg atgtccgcaa tccaagctaa agttgcagaa atgtctgcta
     8701 aaaataaaat atctgaagaa agtaacattc atcaacctct ttctaaagat gaaaaaataa
     8761 tgctaaatga ggcaaaagta ttttattctg ctcgttcgca tttaaaggca gaaaatgtga
     8821 ttcttccgag tgattttatg gagcaaatta aatcaggctg tggagcagaa aaaatggcag
     8881 aaatgatggg atataccaaa gaagattggg agtcaatgaa gtaatatggg gcagattaca
     8941 tttagtaata ttttcacaaa agtgattttt gagaatgtgc tttctgcttc aactaaagag
     9001 aaaataaaac aaatgctttt tcagtctgtg gttgcattac ctcctctcca ttcagaaaga
     9061 aaaatgattt tacagttgaa aaagcaaaaa attaccattt tttatcaagt tattgaggaa
     9121 caatctgtgc aaatcctcgc ttatcaattt ggtggaactg ataaattgac aggtgtgtct
     9181 aaagctcact ttaaaacact agatgccatt tttcaaatga cggatgaaga gaaagaagga
     9241 aaattctaat gtaagcggtt atttttccat atttttttgc aaataacgtt tctcaaattt
     9301 tgcttgatgg cgttcaagca cttccgtcat cgaaatgcca tatttatcgg ctaatacaaa
     9361 aagattatcc atcacatcgc ctaactcttc gattaagtta tctcgttttt gctgttgtga
     9421 agcttcggtt tcgtccggtc gttcacgccc gatttcaatc gctcttactg cacaagccac
     9481 ttcgccgatt tcttctgcta gataagtcac ccgcataaaa gggcttcgtt cataccaacc
     9541 ttgttgttgg taaaactggc gaacccattg ttgatattgt tcaatttgca ttttatttct
     9601 gattcaaaaa gtagggacga aaaatttttc gcccttttat aagaaaatgt atcagggcaa
     9661 atgatgattt gcccctagga gtgttactct gcctcagaga gaataatcca gccactatcc
     9721 agccagtcgc aaatggtgtc tagcattagc tccagctccg cttggtcttg cactttggag
     9781 gcaagtttgt tccacgaaat cgaatctcca ttggcgatac gaatcagtaa tgccatttct
     9841 gcttcgttta actcatcaat ccactcgcca tttacataaa cccgttgagg gttctcggtg
     9901 tagaggattt ttacatttgc atcttgctgt aaccagccgc cggcttcgag aatttcttgc
     9961 acttcgtcag ggtaatattc attgtcagtt tgcaataatt cataacggcg ggagcttgcc
    10021 gcagtggcaa cactgtgagt gagtaactca tcgaactgtt cggaattttg gagcaagtcg
    10081 attaactgat gtttaaacat tttcaccatt ttcggatcaa gcaacgcatt cggctgttca
    10141 ttcggcgtta agcggaacgg aagatgaaat tccgatgaat taatgttgtc ggcttgttgt
    10201 tcgatggttt tgcagagatt ttccagtaaa tcggtcgcat ttggataacg taaaccaaaa
    10261 gagaaagtta aaccttgctc gctttcggaa acaccatagt gagccattcg agacggcaca
    10321 taaagcacat cacccgggtt cattacctcg tccattacca gttcgcccat atcatcaaaa
    10381 atgcggatcg gttggtttgg tttgaattcg gtgcttggat cgcaccattt gcctaattgc
    10441 caacgacgtt gcccatagcc ttgcaccaag aacacatcgt actcatcata atgtttgccg
    10501 acagtgccgt tgagcggtga gcaagaaacc ataatgtcat cacgttgcca ttgcgggata
    10561 aaaccaaaag cctgccaaag ctcgcctagc tcaggagacc attgctcgag gttttgcacc
    10621 attacagacc attgtacagg taactcttcc aaatcttcct caacaagcgg gctggattta
    10681 acagaccatt tctcgttttc gcattgaatc aggcgtgcag tgacttcttc ctctaatgcc
    10741 agttctaaaa tatcttccgg ctcaaacaat ccgacaattt ggggtaagcc gttacgaatt
    10801 aatagcggtc gtttttgcca atattctcgc aaaaaggttt cagggctgat attttcaggt
    10861 aagcagaatg gaatgttcat aatggctcct tgctacgtgc ttctttgaga ttactgatat
    10921 tctacccgag aaatcaggaa aaacgggaaa ttttttatga tttagcgaca tcattcacat
    10981 tttgaagagg aaataatgaa aaaggcgaac attgttcgcc tttgaaattt attttaaacc
    11041 gagttttttc tcgagatagt ggatattggt gccacctttg cggaattctt tatccgcaag
    11101 aatttgagta tgtaaaggaa tattggtttt aatgccttcc acaatggttt cggaaagggc
    11161 attttccatc cggcgaatcg caatatcacg attttcagca aaggtaatca atttaccaat
    11221 cattgaatcg taatgtggcg gcacgctata gcctgagtaa atatgtgaat cccaacgcac
    11281 acctaaacca cccggaacgt gtaaacgggt aattttaccc ggcgatggca agaaggtgtt
    11341 tggatcttct gcgttgatac ggcactcaat ggcgtgacct ttcactttaa tatcatcttg
    11401 tgtgatcgaa agtggtaagc cggctgccac acgcagttgc tcttttacca agtccacacc
    11461 ggtaatcatt tcggttacag ggtgttctac ctgtaagcgg gtgttcattt caatgaagta
    11521 aaactcgccg ttttcatata agaactcaaa agtacctgca ccacggtagc caatttcacg
    11581 gcaggcattg gcacaacgct ccccaataaa ttgacgcaat tcaggtgtaa taccctgagc
    11641 aggtgcttct tccaccactt tttggtggcg acgttgcatt gaacagtcac gctctgctaa
    11701 ataaaccgca ttaccgtgag tatcggcaag tacttggatt tcaatatggc gtggattttc
    11761 taaatatttc tccatataca ccatatcgtt attaaatgcc gctttcgctt cggctttcgt
    11821 cattgcgata gattcttcca aatctttctc gtgatgaacc acacgcatac cacgaccgcc
    11881 accaccacct gatgctttga tgatcaccgg gtagccgata cgtttagcga tttctttatt
    11941 tttcttcgga tcgttaccaa ccggaccgtc agaacccggt acgcaaggca cgccggcttt
    12001 tttcattgca ttgatagcag agactttatc gcccattaag cgaatcacat cagccgttgg
    12061 accaataaag ataaagcctg atttctcaac ttgttcggca aagtttgcgt tttcagataa
    12121 gaaaccgtag cccggatgaa tagcatcggc attcgttact tcggcagcgg caataatcat
    12181 cgggatattt aaatagcttt ttaccgatgg agcaggaccg atacaaatgg tttcatcggc
    12241 taatttaacg tgttttaatt cacggtcggc agttgaatgc actgcaaccg ttttaatgcc
    12301 caattcttta caagcacgca aaatacgcag ggcaatttca ccacggtttg cgataacaac
    12361 tttttctaac atagggaaat tccttgtgtt ctagaaaatg tagggcgtgt tgcacacgcc
    12421 cgttggataa tttaaggaag agcttattca atgatgaata atttttggtc gaattctacc
    12481 ggttcgccat cattaactaa gatttctttt accacaccgg ctttgtctga ctcgatacgg
    12541 ttcatcattt tcattgcttc tacaatacat aacgtatcac cgattttcac ggtttgaccc
    12601 acttcaacga acggttttgc atctgggctt gggctacggt agaaagtccc caccattggt
    12661 gaaagcactg cgtggccact cgtttctgcg gcttttggtg cttcagccgg agctgcaaca
    12721 ggtactgtcg aagctacttg aggtgccgct ggtgcaacaa caggagctgc tgcatattga
    12781 actgcagctg gtgcaacagc tggagcagca cggctaatac gcaccgtacc ttcttcttca
    12841 gacacttcta actcggtaat gccagattct tcaactaatt caataagttt tttgattttg
    12901 cgaatatcca taataagatt ccgtatgtga ttaaataaaa taaaaaagag aactccacat
    12961 ttggggtata aattcaaatg tagagttaaa aaatgctggg cgtagaatac ctgaatttcg
    13021 ctaattttgc gaataaaatc tgataatttt ggcgaaaatg gacgatttta gcattcatcg
    13081 taccagttag gatttttgga aaatggtgac tcacaagcga ttgaaaattg ccaatttttt
    13141 gtaaaaaaca accgcttgtc agattatttc aataaatctt catttttctt gtaattccac
    13201 ttgctaccgg gcttgtcgat atactgaaaa tattcgttgg taaaagtacc ttgagcacaa
    13261 gttactttgt ctcggcggaa atttacttct ttatcaccaa tggcattatt tagggtgtca
    13321 ggaccggtca taacaaacac gccaccttca atccgacgat tctcaatatt gctgataatc
    13381 agctcaaggg tgtcttttag aatccaattt ccagcttccg aagccaagaa aaagttggtg
    13441 tatttatcac ggcgtttaat taacacttcg ctatcatttt cgccaataat tttagagagt
    13501 gggtaaacca aatgcccgtc aatatcaata taaatgccac cttctcggag caaagtaaaa
    13561 attcgccaaa aatcagcttg tgccgcacca tcggttaatt tgctataggc gttaaaggtg
    13621 cgttcatcgg cgttggcctt gatgtatgcc tctcgttctt ctgtgctaac gtagcgataa
    13681 tcgtagctca atgacattag gcggttaaac agataattcg catacattgg caaggtgact
    13741 ttgttagaat aattcgtttg ccaaatggtt ttggtaattt tgctcggaat gcttgattgg
    13801 atttttgccg ggctatattc cggaatggtg aaacgttttt tcggaaaaag tgcatggaac
    13861 ggataactca atattttgac gatattaccg aataaacgga agactccgtt acagatcaca
    13921 ataaattgtt catttttaac agccatttta ttaccttatg cttttaagtg tgaaattgca
    13981 aaatccaacg catattcata gccttttgcc cctaaaccgc aaataacgcc ttttgcaata
    14041 tcacttagat aggagtgatg gcgaaaaggt tcacggctat gaacgttaga caaatgcacc
    14101 tcaacaaacg gaatcgacac actcaataac gcatcacgaa ttgccacgct ggtgtgagta
    14161 aatgccccgg ggttaataat aataaaatcg gtatgctgaa aggcttggtg aatacggttg
    14221 ataatcggct cttcaccgtt ggcttgaaag ctctccagct caacatcttg ggccgcagcc
    14281 aattttttta agtggttttc aatatccgcc aaagtttgcg aaccgtaaat ttgtggctca
    14341 cgtttgccca acatatttaa attcgggccg ttaaggagaa ggattttttt catatttact
    14401 tattcaactt gtaattcata attaagccat tcacggtttt ctttcgcccc caagcgttga
    14461 taaaaacgaa taccaccgtc attccaagga ttttattgtc ttacaatgat gtgccttcgc
    14521 ctcatttttc aaggcttgca ttaacgcctc tcccactttc tgattccgag cagtttcatc
    14581 aacataaagc tctttcatat aaatagcggg gcggttttgg gcagtgtaag gaagaaaata
    14641 ataaaccaat atgccgataa tgttagacgc attttcagcc actaaacagt aaaaatcggg
    14701 tgggcttttg cgaaagccgg attccattac aacttcagga gtaatcgcaa aagaatcgat
    14761 atagtgctca aacacagcca gtttttgcat cagagggaac acttggtggg tatcttgttc
    14821 gaccattttg cggattgtaa atgccataaa attccttaca agcggtaaaa aaatccctca
    14881 cttttgcaaa gcgagggagg ttttaattat tcgtcaatag aacgtaataa gtcgtttaag
    14941 cccactttgc ttaaggtttt ctcatccact tttttcacaa tcaccgcaca gtagaggttg
    15001 tgtgaaccat cttttgatgg aagcgagcct gaaaccacaa cagagcctgc cggcacacgg
    15061 ccgtaatgta cttcgccggt ttcacggtcg taaattttgg tagattgacc gataaacacg
    15121 cccattgaga tcacgcagtt atcttcgaca atcacgcctt ctacgatttc agaacgtgca
    15181 ccgataaagc agttgtcgcc gatgatggtt gggttcgctt gtaatggctc taacacacca
    15241 ccgatgccca cgccaccaga aaggtgaaca tttttaccga tttgcgcaca agaacccact
    15301 gttacccaag tgtccaccat tgtgccttcg ccaacgtatg caccgatgtt cacgaaagat
    15361 ggcattaaca ccacgttttt ctcgatatgt gaacctttac gcaccaccgc acccggcacg
    15421 gcacgaatgc catctgcttt gaattgctct tcggtgtagt tgccgtattt ggttggcact
    15481 ttgtcgtaga atttggtttc tgcaccgtcg atgatttcat tgtcggcaat acggaatgaa
    15541 agtaataccg cttttttcac ccattggttt actacccact cgccatcttt tttctcggca
    15601 acacgcagtg aaccgttgtc taatcctgcg atcacctctt ctactgctgc tttggtttca
    15661 gcatcaacgg ttttcggagt aatttccgca cggcgttcaa atgccgcttc aataatagct
    15721 tgtaaagaca ttgttgtgtt ccttcaatat ataaattaaa acagaaaatt cggctcactt
    15781 tttaacatag aaaggcgttt atttccacaa gaaaaaacat atcgcagaaa tttagtttat
    15841 aattccaagc cacttatttg atacaagcgg tgatttttgt ggaaaatttt gcaaatctgc
    15901 ttaccggtaa aacgcgtgag catttagtgc cgttgccgaa cgctctttcg gataaacatt
    15961 accttcagcc tcaggtggtt gaggcttttt tgacgttgca gcatgctgca aaacaagccg
    16021 gctttaattt acagcctgcc agtacttttc gtgattttga acgccaaaaa gggatttgga
    16081 atgcgaagtt taggggtgag cgtaaggttc acgatgataa cggggttgcg attgatatgg
    16141 cggcgttatc ggatcttgaa aagtgcaaag caatgttgag atggtctgcg gtgccggggg
    16201 caagtcggca tcattgggga acggaaattg atatttttga ccctgattta ttgccggaaa
    16261 atacgccttt attgcttgag ccgtgggaat atttggaagg tggctatttt gccgatttaa
    16321 ccaagtggtt acacgttaat gcggaaacat ttggctttta tttcccgttt gacggtaaac
    16381 attctcgagt gggctttgag ccgtggcaca ttagctatcg cccgatttca aatgaatatg
    16441 agcaacgctt taacgatgaa atcttaaaag ctgcgtggca agatgaagaa gtggcgggta
    16501 aggaatgctt acttgaacat tttgaagaat tactaaaatg atccaattaa ttaacgaaac
    16561 ccaacaacac gacaaattcc gccaaatttg cgacaaatgg cagctggttg aagataaatc
    16621 cagcccgtta gccttagtgc taacggagga acgattggaa ctacgcaaat tagacgaacc
    16681 caagctcggg gcgattgcag tgaattttgt ggacggtacg atggcacatc ggcgtaaatt
    16741 cggtggtggg cgaggtgaag ccgttgcgaa agcggtcggc attaaagggg attatttgcc
    16801 aactgtgatt gatgcgacag ccggtttagg gcgagatgct tttgtattag cagcggttgg
    16861 ttgtaaagtg ctgttagtgg aacgcaatcc aattgtcgct gccttgttgg aagatggttt
    16921 aaaccgagct tatcaagatg tggaaatcgg cagttttatg caagaaagaa tgatcttagc
    16981 cgatgttcga gacatttctc agctagatcc acaatcccaa atggctgagg tagtgtattt
    17041 agatccgatg tatccgcaca aacaaaaaag tgcgttggtg aaaaaagaaa tgcgggtgtt
    17101 tcaacattta gttggggcag atttagattc ggacagcttt ttcttaccgg caaaaggatt
    17161 ggcaaaaaaa cgtgtggtag tgaagcgtcc ggactatgcc ccgtttttag cggagcaaaa
    17221 gcctgatttt tcacacacca ccaaaaatca ccgttttgat gtttatcttt ctcacctgaa
    17281 aaatgattaa aggtgggtca tgcgataggc aagcggttat ttttacagta aaatttacaa
    17341 attatgttat cgtttattta tatttaaaaa ggcggttatc gtgccacaac gtgattatgt
    17401 cgctcgttcc aacgagaaaa aaagtaaaaa aaatcaaaaa gaaagtaatg ttaaaaagat
    17461 tttaatgcgg attatcgggg taattttatt cttcggtatt ggtttatggt ttttgaaatc
    17521 cagtgcacct gtaccaacag ttccgtcagc accgcagcct gctaattcac agactaaaag
    17581 cgaattacca agtcgaccgg aagagacgtg gagctatatt cgtgatttag aaacccgtac
    17641 ggtaattacc gataatagcc aaacttcaca agaacgtttg gcacaattaa cggcggaaca
    17701 aaagcagcaa attgaagaaa gacgcaaaca agaagaagca cgcttagccg ctgagcaagc
    17761 tcaacaaaag cctgcgaccg atgtatctgc tacagaattg gctccgaaaa cagaggaagt
    17821 taagtcgtta acggaagaag aattagcagc aaaaaaagca caggagcaag ctttagcaca
    17881 aaagcaagcg gaagagaaac gcagacaagc agagcttaaa aaacagcagg aagcgaaaaa
    17941 agagcagccg gctaaaattg tagcaagtgc tgcaacactg gcagataaag ctgcaaatca
    18001 ggcgggtaaa tttgggttac agtgcggtgc atttaaaaat aaagcccaag ccgaaaatat
    18061 gcaagcaaga ttggcaatgg caggttttaa cgcccgtatc gcaagcagtg ccgattggaa
    18121 ccgtgttttt gtcggcccga taggtgacag agcggcaact tccagagctc aatccaatgc
    18181 acgcagtgta gcggaatgtg tgattgtggc gatgtaaaat tgattgaaat attgaagaga
    18241 gctataggct ctcttttttt atatcccaat cgcatatttc aaggcttgct ttttgatggg
    18301 tgacacttta tcagccaata ctaaaccgac attgcgggca attttgagcg gtaataaatc
    18361 ggtcttaaac gctttataaa acacgtccat gcctgtttgc atcagtaaat tatccggttt
    18421 acgtttttgc tcatagcgtt gaagcacctg ctcacttgca aaatttttgc cttttttgac
    18481 cgcttgttcg atcacctcaa gcaaggtttt aacatcttta aagcctaaat ttacaccttg
    18541 tccggcaagc ggattaatgg tgtgagccgc atcgccgact aataccaccc cgttttgcac
    18601 atagtgctga gcgtgctggc gggttagcgg gaaagcccct gaagcctgca cctgaatgtt
    18661 gcctaattct gcgttgagcc ggcttgggaa atgttgctca atcgcaactc ccagcttttc
    18721 tgctggaagc attttcagtt cggcaatttt ttgtggtgaa tcgtaccaaa caacgcagcc
    18781 gtgattgccg gaaagcggta aaaaggctcg agggccggtt ggatagaatt gttgccaagt
    18841 gccgcttgga gcaggttttt cggtggtaac ggtggcgagt aaacagtgtt ggcgatattg
    18901 ccaagcggtt aaaccaatgc ccgcccattg gcgaacctgt gaatttgcac cgtctgcggc
    18961 aatcagcagt ggggcagcaa aggtgttgcc atcaaccgtc acttcccaat actcgcccgc
    19021 tttatttgca aaaatttgct caaatccgac cgcttgtagg cagtttggat attgctttaa
    19081 ttgctcccat aacgcaattt gaataacgtt gttttcgacc atatcgccga gcttttccaa
    19141 acccaaatca gctgccgaaa aaagagtgtt gaatccatca atttcccagg tttcgagctg
    19201 ggtataaggg gtgcttctca ttgcaacaat ttgctcccac acgcccaatt tttctaacag
    19261 ttgaatcgag cccatactaa tggcggaaat tcgcaaatcg tagtctgaat caggattaaa
    19321 ggtcggcaga gggtttttct ccaacaaggc taaacgcagc ccctgttttg ctaagcctag
    19381 agctgttgct gccccaatca taccgccacc gattacaatc atatctaatt cagttttcat
    19441 tttttcttcc tttttagtta ggcaaaatac tgctaaaacg ccttgaattg tagtgcattt
    19501 cagattagga tcaaaccgat ttatctattt atctaaggag cgttttatgt cttgcaaaac
    19561 tcatgcaaac cacgagcacg aacacggcga aggctgtggt catactgcta ttaaacataa
    19621 cgaccatatc gactatttac acgatgggca tttacaccac aacaaacacg gcgatcacta
    19681 tgacgaacac gttttagaag tgaatgaaac caacccggca gactgtaatg ccgcccattg
    19741 ctgcggcgac cacgttcatg gtgaggtttg cggacacgaa gctgtgccgc acggcgatca
    19801 tatcgactac atcgtgaatg ggcgtttgca ccaccaacac ggcgaccact gcgatgacca
    19861 cgggccggtt gaaattgtga aataagattt ttgatgggag gttttagcct cccattatta
    19921 tttttgcgat ctcgctcgaa aaattgaaca ttcctgctac ccctcttccg attttccttt
    19981 tatgctctcg gtattgattt ctaataccga aatgtgatga ccttagcgat tgtttacagc
    20041 cgagcctcca ttggcgttga agccccgttg gtgacgattg aagtgcatat tagcaacgga
    20101 caaccaaaat taaccattgt cggcttgccc gaagccacag tgaaagaggc tggtgatcgt
    20161 gtgcggagtg cgttgctgaa cgctaatttt atctatccgc cacaacggat cacgattaat
    20221 cttgctccgg cagatttacc caaagaaggc ggacgttttg acttgccgat tgcgattggc
    20281 attctggcag cctcagggca aattgattct gatttattaa cgcagttcga gtttttgggc
    20341 gagttggcac tgacagggca tttgcgagga gtgcacggca cgattccggc agtgatttct
    20401 gccgataaag ccaaacggca gatgatctta gccaagcaaa atgcgaatga ggcatcgctt
    20461 gtttccaatg ccaccaccta ctttgccggc tcgttgttgg aggtggtaaa tatgctcaac
    20521 aaacgggata aactgccgat ttgtcagcac atttcgcaac attctgccga aattcgaccg
    20581 cttgtcagcc gagatcttac tgatattatc gggcaacaac acgccaagcg agccttaatg
    20641 attgccgctg ccgggcaaca taatttgctg tttctcggac caccgggaac gggtaaaaca
    20701 atgttggcaa gccggctggc ggatttactg ccggaaatga ccgatgagga ggcgattgaa
    20761 accgcctcgg tgaccagcct tgtgcaaaat gagctgaatt ttcagaactg gaaacaacgc
    20821 ccgtttcgtt cgccccatca cagtgcatca atggtggcat tagtgggagg tggctgtgaa
    20881 aactagaaat aaatgtcaaa aacaagaaat aaatgtcaca aatctttctc taatttcgct
    20941 ataattcgat catcaaaact ttaaggggaa atccgttatg ttctacctca ataacgacaa
    21001 tttcaaacga gaaccttatc gtggatattg tccgagtcac cttgaaaagc tttttactaa
    21061 accacacctt gatattagag gtaacggtta ttattaccct gacaatgaat atgttgtagc
    21121 ttggtatgaa tcggacttca ttttcaaatc aagaaaagct caagcacatg caaaagtaaa
    21181 gcttgttaat ttaacttgcc ccaaatacac tggcaaaaac agtgctgaat atttaagcgt
    21241 tttgtatatc tccgttcccc ttaattatct acattatttc actatcggca gtatctggaa
    21301 aggcggtgtc gccaaagagc agtttgctct cgaagaattt tacattacgg ttgaagctga
    21361 aaatcaagat ggtaaacgag aaaacctctc aatcgtttca ttcggtgaga gtgaggaaaa
    21421 cggtttagag aaaccatttg attacacaat ctacactatc cccacagaat atcacaacta
    21481 cggtgatgac ctaaaccgac tactttccat ctcttaccaa aatcaaaaat tcattatcca
    21541 tccgctacat atttttatga tgcactacgg gtactctacg gacatcaagc gtatcttggc
    21601 aacataccca cttgacgagg taagagaacg cctatttata gataaggtcg ttgagaattt
    21661 tgatgtagag cgttatgtcg ttttaccgaa atactttgta aaaaaagatg cgattttcct
    21721 ctatcacttc aaatatgacg aagagactac gggaacaagg gtaaaaaagc ttgtaagcca
    21781 gtttaggtta aatattcaca atcaaacaca tccaatagaa atagattttt ggcacacgca
    21841 aaaggttgaa ttgaaaatcc gtggtgttcg acttggaaat gctgtgttat gcgcagaaat
    21901 tatcggtctt aatcagcccg aaggcgaaga tattacgcta gtgctatctc aatcgaaaag
    21961 agaaaaatcg ggtaataaaa ccgaggaaca aaataacgag gtaaataacg ttcctattac
    22021 cagagtttat acacgagaac ctgaattgga tgagttacca ctcaccgata actccccaga
    22081 taatcgaaca gtcgaataca acaagcgaca atttgaactc ttgggccgtc aacgccaaat
    22141 cagagcgctc aagaaagcac gcgaagagag taatcagaaa atgaaagcct tgactccaga
    22201 tgagatagac tcactgggtg taggtgaatc agatggccga aacggtaagg ttggactcgc
    22261 tttctgtttc cttgatgata cacctgttgg caaaagctcc aaactataca aattatggca
    22321 gcacgcgaag gctgttgcgg aatggaatca cggtgaggcg cactggtaca cgcctaatct
    22381 cggttttaga aatgacgatg aactccttcc cgtttcctta gaaaccaaca aatgtttcga
    22441 ctaccctgaa atcgcgataa ttatccgcct ccaaatatac ggagaaacat ttttcctgat
    22501 tgatttctca atgaagcaaa gagatataag cattcgtggc ttagggtata aacccgatcc
    22561 aaacgaagat tttctctatg aaagtgatat gagcaaaagc gaattatccg aattattgtc
    22621 cgcagttcat catcatgaac atctgccaaa agagtacata gagaaaaaga acaaagaaaa
    22681 aaccaaactc actacattta accatagcga agccgaaagt agtaactggg cttataacgc
    22741 ggtgcaaaaa ttaacttcaa gggcaattac taagccgaca tatttttaat tatttttatg
    22801 aatgcagaac aacgtgtaat tgttactccg acattttggg gtaaattttt tggaaaaatt
    22861 aaaagtgttg aacttcaaca aaacaaagtt atcgtaactg acaaaaagaa taatataact
    22921 gaacatgatt taggaaaaac ttttgacttt ccagctatcc agaagtcttt ctttggtact
    22981 aaattatttt ttaaagacga tagcacagaa gttgttttat caaaacttgc gaaaaaacaa
    23041 actgatagtc tattacttga aatcgaaaaa gtagttgcat ctaatattaa agtgaaagta
    23101 aaagagggtt ttcaacactt tgcaaattta gccgaaaatc aatatctcag agattccgac
    23161 attcctacat taaatgatcg tgtaagattg tcagtgcttt cctatgggga taacaaagaa
    23221 cattttcaaa aatactttga tgaaagtttg gttaagaaaa ttcaatatat ctcttcactt
    23281 tttcctgtaa atgccataaa tgctcaaaca attcgaagag aatacgagca acatgtttta
    23341 aatcatcgca agcatttctt tgatgtcatt gaatctaatc cgcttactga gcaacaaaga
    23401 ttgtcagtca ttcggaacaa cgacatcaat atggttcttg ctgccgcggg gacaggaaaa
    23461 acgtctgtaa tggtcgcaaa agcattggac ttaattgatt ctggtgcttg taaaagcgaa
    23521 gatattttga ttcttgcata caaccgcgat gcagcaaccg aactatccga acgcttacaa
    23581 gaacgtgcac gagaagccaa tttagatctg gaaaatagcc ctagcatctc gactttccat
    23641 gcgcttggcc gaagaattat taaagaatgt ggaaaatcag tccacttgtc aaaatttgcc
    23701 gaagatccga tgaaattaga tagatggttc aacaaatggc tagaggacta catcaaagaa
    23761 gatcctgata atcttgcaaa attcattcaa cttgcatacc aacctatcga tccattccaa
    23821 ttcaaaacga aagacgaata cgatagatat gtaaatgaca atgactatcg aaccttacaa
    23881 ggcgaacgcg ttcgtggtta tcaagagtta ttgatcgcga actggctttt tagccacagc
    23941 gtagaatatg agtatgaacc aaggtatgta tcaaaacgcc gtatcgagat tggttttgac
    24001 tacaaacccg atttctcttt gggtgatggt gtttaccttg aacatttcgg cattgaccga
    24061 caaggtagaa ctcgcgcaga tattaacgcg caggaatata atgccaatat ccaaagaaaa
    24121 cgtgagttac acgccgaaca taatacaaca ttactggaga cataccacta caactgggtg
    24181 gaaaatacgt tatataaacg actagagcaa ttgatgaatg aacagttcat tcctctgaag
    24241 ccaaaatcac aacaagagat tttagatgca ctcaatgaat caggaatctt caaagaaaac
    24301 aaaaatcgct atttgaagtg ccttcaagct attcgtacag agcgattaga ctatcaacaa
    24361 atcttaaaac gtctaaccga tgcgaaaatt gtctatgcta aagaatatgc gacattgctc
    24421 atgcgtattc atgatgccta cgttaaagaa ctacgtagcg caaatgaaat cgactttgac
    24481 gatatgattt tgttggctac tcaactggtg aaaacaggtg agtttaaacc taaatggaaa
    24541 catattttag tcgatgaatt tcaagatatt tctatggctc gattggagtt attgaaagaa
    24601 atttatacta aagggcctcg accgatttgg actgttgttg gagatgactg gcaatcaatc
    24661 tatcgtttct ctggcggtaa actagaagtt actacgcgat ttaatgatat ggtcggctca
    24721 cacacgctaa gtaaactaga aaaaacctac cgctataaca atagtattgc tgacacagca
    24781 ggtcaattca ttatgcaaaa tcctgagcag tatcagaaaa atgttgtaac gcatacaaag
    24841 gtcgctgatt cttgtgttca tttgtatgac tctcatgtcg taaaggatga aaaatcagaa
    24901 gccaatatca gtttaaaagc ctcagcaata ttaaaactga tacgtcaaaa agcacctgaa
    24961 gccactgtcg caattttggc tagataccgt taccttttag aagatgcaaa aagtgtaatc
    25021 aaagataaga atgttaaatt ttggacgttc cacggctcaa aagggttgga agcagattac
    25081 tgcattttac ttggtttctt ccaaggaaaa gtcggatttc ccaaccaaaa taaagaagaa
    25141 gccgttgtag aagcattgct tcctatgcta gacagttttc cacactcaga agaacgtagg
    25201 ttattttatg tggcattaac gcgtgcgaaa aaagaaagtt atttgatcgc agatgcgaac
    25261 gcgccatcag aatttatcaa tgaattatta tcaccgaaat acaagataga tattgtgtca
    25321 aaacgattcc aagaacgtta tcggacaatc tttaaatgta atgaatgtag cactggctat
    25381 ttcgtgatga aacaaggtaa atttggtcag ttctattctt gtacatcagg ggaagtatgc
    25441 cgctcttcgc cacggatttg tgaaaaatgt ggccgtccta tgaagctgcg agaaggtaaa
    25501 tttggtcagt tttggggatg ctcaggatat ggaatcaaag gcgatcaatg tacgaatact
    25561 cgtaagtatt ttatgtaata tattattttg gaggattttg tggatttatt tgaatcaaaa
    25621 aaacaattaa tagagttaat taaaaagtac gatagtgata aagaaattta ttcttcatcc
    25681 tcatacaatg agtctcagtt aagaactgac tttttagacc ctttctttgg tttacttgga
    25741 tgggatataa ctaactcaaa gaataaactc acatctgaaa gagaagtgat tgttgaagaa
    25801 ccactaaaag aaaaagtttc agaaaatgta aaaaaaccag attatacatt tagattcaat
    25861 tctcaaagaa agttttttgt tgaagctaaa aagccaagtg tcaaaatcaa gactgaccct
    25921 ttacctgctc atcaggtaag acgatatggc ttcacagcaa aattaagtat ttctgtttta
    25981 tctaattttg aatatcttgc tatatatgat tgttcagaaa tagttgatgt acaacaagat
    26041 gctctatacg caagggtacg attatatcac tatacagaat atgtgaaatt ttttgatgaa
    26101 attatgaatc tcttaggtag agaaagtgtt tattcaggtc attttgataa tgaatggcgt
    26161 gaaatctctc aaaaaataga aaactctaag caaacagtgg atgaactatt ttttgaacaa
    26221 attaataagt ggcggcatgt tctgggtagt gaattactta aaattaacc
//
LOCUS       DANLXS010000011        25842 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.011, whole genome shotgun sequence.
ACCESSION   DANLXS010000011 DANLXS010000000
VERSION     DANLXS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25842
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.011"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..264
                     /gene="ftsB"
                     /locus_tag="PXD71_000382"
     CDS             <1..264
                     /gene="ftsB"
                     /locus_tag="PXD71_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDL6290543.1"
                     /translation="TFFALLLGFFQYSFWFGKNGWSDYQEVQEEIALLKVENTKLTSR
                     NKLIEAEIYDLKNGVDALEERARLEREMVKENEMFYRIVPKNK"
     gene            282..965
                     /gene="ispD"
                     /locus_tag="PXD71_000383"
     CDS             282..965
                     /gene="ispD"
                     /locus_tag="PXD71_000383"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL6290544.1"
                     /translation="MKRKIIAVIPASGVGSRMQAEKPKQYLQLKGKTILEHTVSIFLE
                     HPHVEKVIVAVARKDPFYSTLELLKSPKIQLVFGGETRADSVLNALQEINTENAWALV
                     HDAARPCLKRSDLDKLLQINDPQGGILAIPAVDTMKRANGTKIAHTEDRSTLWHALTP
                     QFFPAQALKQALSSAFANKLQVTDEASAMELAGYQPQLVSGRSDNIKITRPEDLALAE
                     FYLTHNLEN"
     gene            967..1446
                     /gene="ispF"
                     /locus_tag="PXD71_000384"
     CDS             967..1446
                     /gene="ispF"
                     /locus_tag="PXD71_000384"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDL6290545.1"
                     /translation="MIRIGHGFDVHAFGEDRPLMIAGVEVPYHTGFIAHSDGDVALHA
                     LTDALLGAAALGDIGKLFPDTDMQYKNADSRGLLIEAYRQVQEQGYQVGNVDVTIIAQ
                     APKMRPHIDKMRQVIADDLQCDISQVNVKATTTEKLGFTGRGEGIACEAVALLLKRA"
     gene            complement(1561..2418)
                     /locus_tag="PXD71_000385"
     CDS             complement(1561..2418)
                     /locus_tag="PXD71_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL6290546.1"
                     /translation="MMKSLCKFLSIFNRLLLVCLALFSLNATAVTYPEPPNPFYYITD
                     YTKNTLSQQEWRTLEDALIANCAKTSSQIAVVIIPSTEGEAVSTYAHTLFNKWGIGRS
                     KNNENNGILLLIAKNDRKLFIATGRGLEGALPDAIASSIIRNDITPFFKQEQYAAGIA
                     HGLSAIMAAINGEYAPYASYGEEMQEFDDIDGLLFFLFAGIVIFLIFRPRGNSTYISP
                     SSMDQLGQILRESQRRGGGFGGGLGGGFDRGSGGFGGGFGGGFGGSRGSGDSFGGGSS
                     GGGGAGGSW"
     gene            complement(2415..2873)
                     /locus_tag="PXD71_000386"
     CDS             complement(2415..2873)
                     /locus_tag="PXD71_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL6290547.1"
                     /translation="MGLFSFLSNKLPIDTQRIEQAIADLEQKTSAELRVVVERKAKIG
                     KTDNAAMLRASELFDELKMRETAEHNGVLIYLSFKPHYAAIIGDEGIHQKVDNDFWQK
                     IYATMCLDCQQGDYTQAICDAIKMVEAPLAHHFPYQADDKNELSNEVVVK"
     gene            complement(2955..3539)
                     /locus_tag="PXD71_000387"
     CDS             complement(2955..3539)
                     /locus_tag="PXD71_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDL6290548.1"
                     /translation="MKKIFWILLIAMLAVGGYSLTKYNDLMRAEEDINSVWGNVESAY
                     QRRADLIPNLVNTVKGQANFEKETLTSVIEARAKATAVTIDPSNATEEQMAKFQEAQQ
                     GVNSALSRLLVSVEKYPELKAHEAFLNLQAQLEGTENRINVERNNFNEKVKEYNKQVR
                     EFPTKLAAMIMGFKAKPQFKSEAGSDKAPTVNFN"
     gene            3717..4580
                     /gene="rarD"
                     /locus_tag="PXD71_000388"
     CDS             3717..4580
                     /gene="rarD"
                     /locus_tag="PXD71_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL6290549.1"
                     /translation="MLKGILASLAANVLFGAVYYFAILLRPLEGEAMFGFRIVVVIPF
                     ILLAIILFKQQSAFADLLQKIRQNPPLVLVILLLALNTGVQLWLFLWAPNHGQALQVS
                     FGYLLLPIVAVALGKMVFKEHFTRLKWLSLLFAVIGVGSSLFFNSTISWATWVAGLGY
                     PLYITLRRYFQINTLATFFVELLLVLPVALYYLSQVEMTPIVAENPQIYFYLGLLGLV
                     SGSAFILYILSSNLLPINVLGLLGYVEPLVMLLISFAIGEKLDANSYILMVCLLVSIG
                     LLSWDSFRSKV"
     gene            complement(4733..5251)
                     /locus_tag="PXD71_000389"
     CDS             complement(4733..5251)
                     /locus_tag="PXD71_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HDL6290550.1"
                     /translation="MILLRGDTMQFIEFTDPDSGISYQYSEFTIANVAFIINFCSDAD
                     VISTLSALGKDITNYINTYSCCTIKFMAKEHLENSGSNIDIYAPAANHQFKRKEIIAL
                     QETLERLLFEHYVRFTPESYLFIAERDSLNRMYQRMCVPRCDFMQSFQVVYPLGVNQD
                     CFILITPKGNLK"
     gene            complement(5418..7082)
                     /locus_tag="PXD71_000390"
     CDS             complement(5418..7082)
                     /locus_tag="PXD71_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL6290551.1"
                     /translation="MNISFVFPQTLGNARRFLVNEMLSAAAKQQGHNVVAANQADFVV
                     LFDNAIPQEAAGKQGAILDLEQAFAQPEASLKQAVENAQTFANATATTAVSAVSSGVK
                     NIVAVTACPTGVAHTFMSAEAIENYAKAQGWNIKVETRGQVGAGNPISAEEVAAADLV
                     FVAADIDVDLEKFKGKPMYRTSTGLALKKTTQEFEKAFAQAKIYEGGVAKADNAEAPT
                     SEKKGLYKHLMTGVSHMLPLVVAGGLLIAISFMFGIEAFKDETIAGGLPKALMDIGGG
                     AAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIVAGFLAGYTVKFL
                     NGAIQLPASLTSLKPILILPLLGSAIVGLLMIYVINPPVKALMDALVEWLNTMGQTNA
                     IILGIILGAMMCTDMGGPVNKAAYTFGVGLIASQQYMPMAAVMAAGMVPPIGMAIATL
                     IARSKFNTNQRDAGKASFVLGLCFISEGALPFVAADPVRVIASSILGGATAGAISMAL
                     GITLQAPHGGLFVIPFVSQPLMYLAAIAIGSLVTGVVYAAIKQKAE"
     gene            complement(7108..8052)
                     /gene="fruK"
                     /locus_tag="PXD71_000391"
     CDS             complement(7108..8052)
                     /gene="fruK"
                     /locus_tag="PXD71_000391"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL6290552.1"
                     /translation="MRIATVTLNPAFDLVGKLQRIEIGEVNTVETLGLYPAGKGINVA
                     KVLADLGTKLSVTGFLGEENQGDFVNAFKHNDLADEFYRINGKTRINVKITETEADVT
                     DLNFLGFEISESDWEKFVTLSHQWESKFDLVAVCGSLPRGVTPEKFASWLESLHRQGL
                     KVVLDTSNAALTAGLTAHPWLVKPNRRELEVLVGHSLNSIEEIISAAKQLRSRGIENV
                     IISMGEQGSLWLNHEGVLQARPPRCENVVSTVGAGDSMVASLIYGLSQGWSKEQTLRF
                     ASATSALAVVQSNVGIADRAALDDIIKQTDITVISENH"
     gene            complement(8066..9544)
                     /gene="fruB"
                     /locus_tag="PXD71_000392"
     CDS             complement(8066..9544)
                     /gene="fruB"
                     /locus_tag="PXD71_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDL6290553.1"
                     /translation="MLNLSAKNIRLNGSASNKEEVIRFVAAELVAKGNVAAGYEVGML
                     NRETQTSTFLGNGIAIPHGTLDTRDLVKETGVQIIQLPQGVEWGEGNTAYVVIGIAAK
                     SDEHLTLLRQLTTVLSDEEAAATLAKTQNLDEFIAVLSGKKNLPALSQELISLNIESS
                     SPITLSAINAAKLQEQGYVSQAFISDVVANKALHLGNNLFVVDSAKGNQANGIAIARN
                     TQGQTLLTVAETDNALQPLLTQLLKPEVRSQFAHFSAEQMLGLLKGESVASTPVTNVE
                     ASGNQVVGTFTIRNDNGLHARPAAVLVQTVKPFSSKISVENLDRNTAPASAKSAMKVV
                     ALGASQGHRLRFVAEGEDAQQAIEALQQAIINGLGESVSFVPAVADTIESIAVSAIPA
                     EETATAVDANTKEATFVIKNEHGLHARPSAVLVNEVKKFNAKIEVQNVDKNSPLVSAK
                     SLMKIVALGVTKGTTLRFVASGDDAEVALAAIGAAIEAGLGE"
     gene            9832..12054
                     /gene="glgB"
                     /locus_tag="PXD71_000393"
     CDS             9832..12054
                     /gene="glgB"
                     /locus_tag="PXD71_000393"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HDL6290554.1"
                     /translation="MQDYTYSEKDAFFVERLSSGSCNDPFSYLGIHPSNNGVIIRVFL
                     PSAIACQVIDLNGKPVSSMLKIDDRGFFIAELIGKKTDFSYRLLVEYPDSTVDIEDPY
                     RFKTHIQELDNWLFAEGKQLRPYEKLGAHLVTQNEVGGVHFSVWAPNAQRVSVVGDFN
                     HWNGLVHSMRFHPDSGIWDIFLPNVKKGECYKFEVLDRNGVLRLKSDPYAFASQFRPE
                     TASIVSGLPSIVPAEKTCRNANAPDKPISIYEVHLGSWRRNIENNYWLSYEELADELI
                     PYVKDMGFTHIELLPISEFPFDGSWGYQTTGIYSPTSRFGTPEGLSYFIQKAHDKGIG
                     VILDWVVGHFPVDEHGLVYFDGTHLYEHSDPKEGYHKDWNTLIFSYGRNEVSNYLSGN
                     ALYWIERFGIDALRVDAVASMIYRDYSRSDGEWIPNKYGGRENLEAIDFLRHTNNILR
                     DESQGGASIAEESTSFAGVSHSTERGGLGFNYKWNMGWMNDTLRYMQKDPIYRRYHHN
                     LMTFGMTYQYSEQFVLPLSHDEVVHGKGSLLNKMPGDCWQKFANLRAYYGYMWGYPGK
                     KLLFMGNEFAQGKEWNFAESLDWYLLGEEGGEWHKGVLGWVKALNHLYTQTPALFEQD
                     YNPEGFEWLVVDDHDNSVFAFERKAKNGDSVIVVSNFTPIVRENYRFGVNELAEYQEI
                     LNSDLACYCGSNVSNANRFASELIASHGKAYSLSITLPPLSTIFLKRAADKKTKQNKT
                     "
     gene            12078..14090
                     /gene="glgX"
                     /locus_tag="PXD71_000394"
     CDS             12078..14090
                     /gene="glgX"
                     /locus_tag="PXD71_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDL6290555.1"
                     /translation="MHYFRGKPFPLGNKLDRQAGEFGTNFAIVSQNATAIELCLFDEN
                     HHETRIPMFSSGDTWHIFVAGVQAGTKYGYRVKGIQDEQAGLLFNPQKLLTDPYAKAI
                     EGKPDLSSAEAAAWYKWNDERDNASLAPKSIVIDEHFDWENDSQLFTPWAETIIYEMQ
                     VKGFSQRNFQIPSDIRGTFAGLAHPVSIDYLKTLGITAVELLPVTYHIDETHLQKQGL
                     VNYWGYNVLGNFAIDPNLAADKQNPLKEFKQLVKTLHQAHIEVIMDIVFNHTAESDKN
                     GPMLSFRGIDNTAYWLDKQGEYENWTGCGNTLNLSNPYILCWAIDCLIYWVEECHIDG
                     FRFDLASILGRTPSFSEKADFFTALKSHSKLTHIKLIAEPWDIGVEGYQLGQFPEPFY
                     QWNDCYRDDIRRFWLQNQGDWGLLARRLAGSDDVFTKYSVSKSINFITAHDGFTLQDL
                     TSYQQKHNWANGEQNRDGHNSNYSNNHGVEGQTADELVKKQREITACSLLATLFLSAG
                     VPMLLAGDEIGHSQQGNNNSYCQDNEITWLDWENMNHTRLDYVRSLIRLRKKIALLSK
                     QNNWWQEDSVQWLNPSAQLMNIDDWHNQHNGLVVLLQEEWLLLFNANRQPQPFLLPQG
                     NWKVVLEQAQGILMTDTQAVTLNNMGVCVLQKSDVMFANKLETQYA"
     gene            14083..15396
                     /gene="glgC"
                     /locus_tag="PXD71_000395"
     CDS             14083..15396
                     /gene="glgC"
                     /locus_tag="PXD71_000395"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDL6290556.1"
                     /translation="MLNQESTALVARQDLTNDTLVLILAGGRGSRLYELTDKRAKPAV
                     YFGGSRRIIDFALSNCINSNLLKIGVITQYAAHSLLRHLQRGWSFLPYERNQYIDMLP
                     ARQQLDENTWYRGTADAVYQNMAMMKSHYRPKYVVILAGDHIYKMDYTQMLKDHVDQG
                     AKCTVGCIEVPRSQATEFGVMAVNEKLKVKEFVEKPADPPAMPGKPDSSLASMGIYVF
                     NADYLYEMLEKEASTPGTSHDFGKDIIPKAVEQGVLYAHPFERSCKGRNASGAIYWRD
                     VGTIDSYWAAHMDLVSEDPELDLYDESWPIHGRPQQTAPARFFYNRARARTLDNSLIA
                     GGCVITDAEISNSVLFNRVHVEAESVIEHTVILPQVTIGKNCVIKRAVIDRHCVIPDG
                     LEIGVNAEEDAKRFRVSKNGIVLVTQSMLDKLAGKEANSDIKYTI"
     gene            15519..16955
                     /gene="glgA"
                     /locus_tag="PXD71_000396"
     CDS             15519..16955
                     /gene="glgA"
                     /locus_tag="PXD71_000396"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDL6290557.1"
                     /translation="MKVLHICSEMYPLIKTGGLADVMGALPFAQQQAGNDVRVLIPYY
                     PKVAQTLGETVEVATIGTFAGTVSVRFAYFNGLGVYVIDAPHLFMRDGNPYHDSNYQD
                     YPDNYKRFALLGYIGAQLAQGLDHWWGTAQVLHAHDWQAGLACAYLKSWGSNVKTLFT
                     IHNIAYIGRFNAFHLAELGLPWEFFHAEGLEFYGEISYLKAGLYFADRITTVSPTYAR
                     EITEEIAGAGLHGLLATRQYQGRLRGILNGVDAEVWNPETDTHIVVNYRANYMHGKAK
                     NKAELQRIFGLPEDKEALLMVMVTRLTEQKGADFLLANIDTLMQHKVQIIVLGSGSPD
                     LENALRYFQHKYPNQIGVKIGYDEALSHQIIAGGDLILVPSRFEPCGLTQLYGLKYGT
                     LPLVRRTGGLADTVVDSHKESIENRSATGFVFDYPDAPAFLEAAMRAISLWQKQKLWS
                     SVRQNALAQDFGWAKVAEQYQNLYHEII"
     gene            17050..19550
                     /locus_tag="PXD71_000397"
                     /pseudo
     CDS             17050..19550
                     /locus_tag="PXD71_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
     gene            complement(19632..20348)
                     /gene="trmJ"
                     /locus_tag="PXD71_000398"
     CDS             complement(19632..20348)
                     /gene="trmJ"
                     /locus_tag="PXD71_000398"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDL6290558.1"
                     /translation="MQILNQIQIILVETSLPANIGSAARAMKTMGLTHLRLVAPKQPL
                     DEQAEALAAGAKDVLDNAQCYATFDEAIADCQLVIGTSARLRHLQNTLIEPRDCGELV
                     VNRTNLGKVAIVFGRERVGLTNEELLKCQYHLNFPTNPDYGSLNLAMAVQLASYEIRM
                     AYLQNSGEKQPLAADKDYPTAQALEHFFAHTERLYTELGFIRNDSVMLKLRRLYQRAE
                     LETNELNLLRGMLTAVEKAN"
     gene            20528..22066
                     /gene="pntA"
                     /locus_tag="PXD71_000399"
     CDS             20528..22066
                     /gene="pntA"
                     /locus_tag="PXD71_000399"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDL6290559.1"
                     /translation="MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEQDAGFKAS
                     FEDNTFLNAGANIGTQQEVWNADIIFKVNAPTEAEIELIKEGVTLVSFIWPAQNPQLM
                     EKLQSKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
                     GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEE
                     EGGSGDGYAKVMSEEFNRRAMALYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMK
                     PGSVIVDLAAATGGNCEYTKAGEIVVTDNQVKVIGYTDFPARLPTQSSQLYGTNLVNL
                     LKLLAPNKDGQIEINFDDVVLRGVTVVRDGELTWPAPPIQVSAQLQKQAAAPQAVKKE
                     EKPADPRVKYGVMAAAGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHT
                     PLMAVTNAISGIIIVGAVLQIAQPTGNFFIDILAFIAILVASINIFGGFKVTQRMLAM
                     FRKG"
     gene            22081..23526
                     /gene="pntB"
                     /locus_tag="PXD71_000400"
     CDS             22081..23526
                     /gene="pntB"
                     /locus_tag="PXD71_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDL6290560.1"
                     /translation="MSFGFVTAAYIIAAILFIMSLAGLSKHETAKAGCWYGIVGMAIA
                     LVATIFGPQSHGTIWILIAMAIGGFIGVKKALKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYGLHTNALMPANLDETAQAAFLAEQATLANIHNVEVFLGIFIGAVTFSGSLVAFG
                     KLSGKLFGRKVSSAALNIPHKHKWNLAAIIVSVFLMIVFLNNPHNIFPVLIMTIIALV
                     FGWHLVSSIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYI
                     MCKAMNRSFISVIAGGFGNEVQSGKGDEEYGEHRETKAEEVAEMLKNASSVIITPGYG
                     MAVAQAQYPVAEITAKLREKGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDE
                     INDDFEETDVVLVIGANDTVNPAALDDPSSPIAGMPVLEVWKAQNVIVFKRSMAVGYA
                     GVQNPLFFKENTQMLFGDAKERVDDILRALN"
     gene            complement(23593..24519)
                     /locus_tag="PXD71_000401"
     CDS             complement(23593..24519)
                     /locus_tag="PXD71_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6290561.1"
                     /translation="MKPIFLEIRHLRTLIALKESGSVSLAAKRVHLTQSALSHQIKLL
                     EEQYDLTLFERKSSPIRFTPAGERLIKLAYDILPKVIEAELDLARVKQGEVGEVRVAV
                     ECHTCFDWLMPAMDTFRQHWPLVELDIVSGFHTDTVGLLLSHRADLAIVAEEEDSAGI
                     IYKPLFSYEMVAICSKDHPLATKDVWEAEDFKDETLITYPVPDDMLDLLRKILGPAGI
                     KVTRRNSELTIAIIQLVASRRGIAALPYWAVKPYLDRGYVVARKITQQGLYSTLYGAI
                     READATLAYLDDFHDTVKSQSFATLPGLMVLE"
     gene            complement(24629..25744)
                     /gene="asd"
                     /locus_tag="PXD71_000402"
     CDS             complement(24629..25744)
                     /gene="asd"
                     /locus_tag="PXD71_000402"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL6290562.1"
                     /translation="MQNVGFIGWRGMVGSVLMDRMVEENNFANINPVFFTTSQAGQKA
                     PVFGGKDAGELKNAFDIEELKKLDIIVTCQGGDYTNEVYPKLKETGWNGYWIDAASAL
                     RMKDDAIIVLDPVNQHVISEGLKNGIKTFVGGNCTVSLMLMAIGGLFEKDLVEWVSVA
                     TYQAASGAGAKNMRELLSQMGELEDSVKAELANPASSILDIERKVTAKMRAEDFPTEN
                     FGAALGGSLIPWIDKLLPETGQTKEEWKGYAETNKILGLSDNPIPVDGLCVRIGALRC
                     HSQAFTIKLKKDIPLAEIEQIIAAHNEWVKVIPNDKETTLRELTPAKVTGTLSVPVGR
                     LRKLAMGGEYLAAFTVGDQLLWGAAEPVRRILVQLTQ"
BASE COUNT         7304 a         5142 c         5774 g         7622 t
ORIGIN      
        1 actttttttg ccctattact cggctttttc caatatagct tttggtttgg aaaaaatggt
       61 tggagtgatt atcaagaagt gcaagaagaa attgctttat taaaagtaga aaacacaaag
      121 cttacttcac gcaataaact aattgaagcc gaaatttacg atttaaagaa cggcgtagat
      181 gcacttgaag agagagcaag acttgaacga gaaatggtaa aagagaacga gatgttctac
      241 cgaattgtac caaaaaacaa ataatacata attgatatat tatgaagcga aaaattattg
      301 cagtaattcc ggcgtcagga gttggtagcc ggatgcaagc agaaaaacca aaacaatatt
      361 tacaattaaa gggtaagact atcttagagc atacagtgtc tatttttctt gaacaccctc
      421 atgttgaaaa agtgattgta gcggtggcaa gaaaagaccc tttttattcc acattggaac
      481 tcctaaaatc ccctaagatc caacttgttt ttggaggaga aacgagagca gattcagtgc
      541 taaatgcctt acaggaaatc aacactgaaa atgcttgggc attagttcac gatgcggctc
      601 gcccttgcct caagcggtca gatttagata aacttttgca aattaacgat ccgcaaggtg
      661 gcattctcgc cattccagcg gtagatacga tgaaaagagc aaacggcacg aaaatcgcac
      721 acacagaaga tcgctcaacc ttatggcacg ctttaacccc acaatttttt cctgctcaag
      781 cattaaaaca agcattatcc tctgcttttg ccaataagct tcaggtaaca gacgaagcat
      841 ctgcaatgga gcttgcaggc taccagccac aattggtttc aggtagaagt gataatatca
      901 aaatcactcg ccctgaagat ttagccttag cggaatttta ccttacacat aatttggaga
      961 actaaaatga tacgtattgg acacggtttt gatgttcacg cttttggcga agatagacca
     1021 ctgatgattg ctggggttga agtgccttat catacaggct tcattgcgca tagtgacgga
     1081 gatgtagcgc tgcacgcttt aacagatgcg ttgcttggcg cagcagcttt aggtgatatt
     1141 ggcaaattat tccctgatac tgatatgcaa tacaaaaatg ccgatagtcg tggtttgttg
     1201 attgaagctt accgacaagt gcaagaacag ggctatcaag tcggtaacgt cgatgtaacc
     1261 attattgctc aagctccgaa gatgcgaccg catattgata aaatgagaca ggtgatcgca
     1321 gacgatctac aatgcgatat atcacaagtg aatgtaaaag ccactacaac agaaaaatta
     1381 gggtttaccg ggcgaggtga aggcatagct tgtgaagccg tagcattgtt actaaaaagg
     1441 gcatagctta gagaagtgag gaagcggtct tattttcatt agcgttttgt gcgtttagcg
     1501 aaaaatacgc aatttttttg agaaatatga ccgcttgtat tgttttaaat tgggcggaaa
     1561 ctaccagctt cctccagcgc ctccaccgcc tgatgaacca ccaccgaagc tgtcgccact
     1621 gcctctgctt ccgccaaaac caccaccgaa gcctccgcca aatccacctg agcctcgatc
     1681 aaagccacct ccgagaccac cgccaaagcc cccacctctg cgttggcttt ctcttagtat
     1741 ttgtcctagt tgatccattg agctgggact aatataggta ctgtttcctc gtgggcggaa
     1801 aattaggaaa atcacaatgc cggcaaacaa gaaaaagagt aagccgtcta tgtcatcaaa
     1861 ttcttgcatt tcttcgccat aacttgcata aggggcatat tccccgttaa tggctgccat
     1921 aatggcggat aagccatggg cgataccggc tgcatattgc tcttgtttga agaatggcgt
     1981 gatgtcattg cggataatgc tcgatgcaat cgcatcaggt aacgctcctt caagcccacg
     2041 tccggtggca ataaagagtt tgcgatcatt tttggcaatt aataacaata taccattgtt
     2101 ctcgttattt ttgctccgtc cgattcccca tttgttaaac agggtatgag cataggttga
     2161 aactgcctcg ccttccgtgc ttggaataat caccacggca atttgcgaac ttgtcttggc
     2221 acagttagca attaaggcat cttctaatgt ccgccattct tgttggctca aggtgttttt
     2281 ggtgtagtcc gtaatataat aaaacggatt aggcggctct gggtaggtca ccgctgtggc
     2341 atttaggcta aacagagcca agcacaccaa taacaagcgg ttgaaaatag atagaaattt
     2401 gcaaagggat ttcatcattt gactaccacc tcgtttgaaa gctcgttttt atcatcagcc
     2461 tgataaggga aatggtgagc gagcggagct tccaccattt tgattgcatc acaaatggct
     2521 tgggtgtaat cgccttgttg gcagtccaaa cacattgtgg catagatttt ttgccaaaaa
     2581 tcgttatcga ctttttgatg aatgccttca tcgccaataa tcgcagcgta gtgaggcttg
     2641 aatgaaaggt agatcaacac accgttatgt tcagccgttt cacgcatttt taattcatcg
     2701 aaaagctcac ttgctcgcaa catagcggcg ttgtcggttt tgccgatttt ggcttttcgt
     2761 tccaccacca cacgcaattc ggcagaggtt ttttgctcta aatcggcaat tgcttgttca
     2821 atgcgttgcg tatctatcgg taatttgttg gagagaaaag aaaataatcc catttgttcc
     2881 ccttaaaaat aaagcaataa gcaccggatt tgaaagagct tattgctttt aatatgaata
     2941 aattgaaact aaaattagtt gaagtttacg gttggtgctt tatcagaacc tgcttcagat
     3001 ttaaattgcg gttttgcttt aaagcccata atcattgccg ctaatttagt cgggaattca
     3061 cgcacttgct tgttgtactc tttcactttt tcgttaaagt tattgcgttc aacgttaatg
     3121 cggttttccg taccttctaa ttgagcttgt agatttaaaa acgcttcgtg ggcttttaat
     3181 tcagggtatt tctcaactga aaccaataag cgtgaaagag cagagtttac accttgttgg
     3241 gcttcttgga atttagccat ttgctcttca gttgcattgc ttgggtcaat ggttaccgca
     3301 gttgcttttg cacgagcttc aatcacagag gttaaagttt ctttctcaaa gttagcctga
     3361 ccttttacgg tattcactaa gtttggaatt aaatccgcac ggcgttggta agccgattca
     3421 acatttcccc atacggagtt gatgtcttct tctgcacgca ttaaatcgtt gtattttgtt
     3481 aaagaataac cgccaacagc aagcatggcg atcagtaaaa tccaaaaaat ctttttcatt
     3541 atgaagtcct ataatataaa taaaagagtg aaaaattggg agcattgtat cgggaaaaat
     3601 cgaatatagc tagcttactt acaaattttt tcatactatg gggcgtagat aagatttgta
     3661 aatttttgtt caaagtcaac cgcttgttgt ttgattgttg ataagaggaa agccaaatgc
     3721 tcaaaggaat tttagcttcg cttgcagcca atgtgctgtt tggagcagtt tactattttg
     3781 cgatattgct tcgcccgtta gagggagagg cgatgttcgg ctttcggata gtggtagtca
     3841 ttccgtttat tttgctggcg attatattgt ttaaacagca atcggctttt gcggatcttt
     3901 tgcaaaaaat tcgacaaaat ccaccgcttg tattggtcat tttgctcctt gctctcaaca
     3961 ccggcgtgca gctttggctg tttttatggg ctcctaatca cgggcaggcg ttgcaggtgt
     4021 cgtttggtta cttgctttta ccgattgtgg cggtggctct aggaaaaatg gtgtttaaag
     4081 aacattttac ccgcctgaaa tggctttctc tacttttcgc agtcatcggt gttggcagta
     4141 gtttgttttt taacagcacg atttcgtggg cgacttgggt agctgggttg ggttatccgc
     4201 tttatattac ccttcgccgt tattttcaaa tcaacacgtt agctactttt tttgtggagt
     4261 tgttactggt attacccgtt gcactttatt atctcagcca agtggaaatg acgccaatag
     4321 tggcagaaaa tccgcaaatt tatttctacc tcggattgct ggggctggta agtggcagtg
     4381 cttttattct ttatatcctc tcgagcaatt tattaccgat taatgtatta ggtttactgg
     4441 gctatgttga accgttggtg atgttgctga tttcttttgc catcggcgaa aaactcgatg
     4501 ccaattccta tattttgatg gtctgtttgc tggtttcgat tggattattg agttgggata
     4561 gttttcgtag caaggtgtga taattaagga aaaataaatc gcttgtagag caagcggttt
     4621 aaagattatt tgaccggttt caatagacct gctttttttg ctcgctgata ggcttgctta
     4681 aaagcgatta agccttcttg ctttaaacga acggaaactg gacgttgttg attcatttta
     4741 gatttccttt tggcgtaatt aaaatgaaac aatcttgatt aacgcccaag ggataaacca
     4801 cttggaaaga ttgcataaaa tcacatcgtg gcacacacat acgctgatac attcggttaa
     4861 gtgaatctct ctcggcaatg aagagatagc tttctggtgt aaaacgtaca taatgctcaa
     4921 ataataaacg ctcaagggtt tcttgtaaag caattatttc tttacgtttg aactgatgat
     4981 tcgctgcggg ggcataaatg tcaatattag aaccgctatt ttctagatgc tctttcgcca
     5041 taaatttgat tgtgcaacag gaataggtgt ttatatagtt tgtgatatct ttgcctaatg
     5101 cggacaaagt cgatataaca tcggcatctg agcagaaatt aatgataaat gcgacattag
     5161 caatagtaaa ttcactgtat tgataagaaa ttccactatc aggatcggta aattcaataa
     5221 actgcatagt atctcctcgt agcaaaatca taacgagttg atattattcg taaagtgatg
     5281 aaaatgattt tttcgatctc gatcgcaaaa ataatatttt agacttgata tttcgatgaa
     5341 atagaattca gtaagtacca atatgatata aaaacggagc gtttcgctcc gtttttgttt
     5401 gtatttgtgt ttgtgcttta ttctgctttt tgtttgatag cggcatagac tacgccggtg
     5461 actaatgagc cgatggcaat agcagctaag tacattaacg gttgtgaaac gaatggaatc
     5521 acgaataaac cgccgtgtgg ggcttgtagg gtaatgccta gtgccattga aattgcaccg
     5581 gcggttgcac cgcctaagat tgagctggca attacacgca cagggtctgc cgccacgaac
     5641 ggtaatgccc cttctgaaat aaagcataaa cctaacacga atgaagcttt acctgcatca
     5701 cgttggttgg tgttgaattt gctacgagca atcagggtcg caatcgccat accaattggt
     5761 ggaaccatac ctgccgccat caccgctgcc attggcatat attgttgtga agcaattaaa
     5821 cccacaccaa aggtataagc cgctttattc accggaccac ccatatcggt acacatcatc
     5881 gcaccgagaa taatacctaa aataattgca ttggtttgcc ccattgtgtt taaccattcc
     5941 actaaggcat ccattaatgc tttcaccggt gggttgatga cataaatcat cagtaagccg
     6001 acaatcgctg agcctaagag tggtaaaatc aaaataggtt taagtgaggt taaacttgcc
     6061 ggtagctgaa tcgcaccatt taagaatttt acggtataac cggctaagaa accggctacg
     6121 ataccaccta aaatccctgc tcctgcggtg gtagcaagca taccgccgat aagaccgaca
     6181 gctaaccccg gacggtctgc aattgagaac gctacatagc ctgcgaatac cgcaatcatt
     6241 aagtggaatg ccgcaccgcc accaatatcc attaaggctt tcggcaagcc gcctgcgatg
     6301 gtttcatctt taaaggcttc aattccgaac ataaaggaaa tagcgatgag caagccacct
     6361 gctaccacca gcggtaacat atgtgataca ccggtcatca agtgtttata taaccctttt
     6421 ttctcagaag ttggagcttc agcattatcc gcttttgcca caccaccttc gtaaattttc
     6481 gcttgagcaa aggctttttc aaattcttga gtggttttct tcaatgctaa accggttgag
     6541 gtgcgataca tcggcttgcc tttgaatttt tccaaatcga catcaatatc ggcagctaca
     6601 aagactaaat ctgccgctgc gacttcttcg gcggaaattg gatttcctgc tcctacttga
     6661 ccacgggttt caaccttgat gttccaaccc tgtgctttgg cataattttc gattgcttcc
     6721 gcagacataa aagtatgagc cacgccagtt gggcaagcgg taacagcaac gatatttttc
     6781 acaccagatg aaaccgctga aaccgcagtg gttgcggtag catttgcaaa agtttgtgca
     6841 ttttcgaccg cttgtttgag actggcttca ggttgagcaa aggcttgttc taagtccaaa
     6901 attgcccctt gcttgccggc agcttcttgt ggaattgcgt tatcgaataa caccacaaaa
     6961 tcagcttggt ttgccgccac aacattgtgt ccttgttgct tggcagcggc agataacatt
     7021 tcattcacta agaaacggcg tgcgttgcct agtgtttgag gaaatacaaa agaaatattc
     7081 atattggttt gttcctttga ttgaggttta atgattttct gaaatcacgg taatgtcggt
     7141 ttgtttaata atatcgtcta acgccgcacg gtctgcaatc ccaacgttac tttgaacaac
     7201 agctagagca gaagtagctg aggcaaaacg caaggtctgc tctttgctcc aaccttgaga
     7261 taagccgtag atcaagctgg caaccattga atcaccagca cctacagtgc taactacatt
     7321 ttcgcaacgt ggtgggcggg cttgtaacac gccttcgtga tttagccaaa gagagccttg
     7381 ttcgcccatt gatataatta cattttcaat accacgggaa cgtaattgtt tagctgcact
     7441 aataatctct tcgattgagt tgagtgaatg cccgaccagc acttccagct ctcggcggtt
     7501 tggttttact aaccaagggt gagcggttaa gccggcggtg agagccgcat tgctggtatc
     7561 taacaccact tttaagcctt ggcggtgtaa agattccaac caagaagcaa atttttccgg
     7621 cgttacgcca cgagggagag atccacacac tgcaactaag tcaaatttgc tttcccactg
     7681 atgagataat gtgacgaatt tttcccaatc gctttccgaa atttcaaagc cgagaaagtt
     7741 cagatcagtt acatcagcct cggtttcagt gattttgaca ttaatgcggg ttttaccgtt
     7801 aatacggtaa aattcatcag ccaaatcatt gtgtttaaag gcatttacga aatcgccttg
     7861 attttcttcg cctaaaaagc cggtaaccga aagttttgtg cctaagtcag ctaaaacttt
     7921 agcaacgtta atacctttgc cggcagggta taaaccaagg gtttctacgg tgttcacttc
     7981 gccgatttca atacgttgaa gtttgccgac taaatcaaag gcagggttga gggtaacggt
     8041 tgcaatacgc ataatgattc ctcgcttatt cgcctaaacc tgcttcaatc gcagcaccga
     8101 ttgccgcaag tgctacttcg gcatcatcac cgcttgccac aaagcgtagc gtcgtgcctt
     8161 tggttacgcc aagggcaaca attttcatta agcttttggc acttacaagc ggtgaatttt
     8221 tatcaacatt ttgcacttca attttggcgt tgaatttttt cacttcattc accaacaccg
     8281 cacttgggcg agcgtgtaaa ccgtgttcgt ttttaattac aaaagttgct tctttcgtat
     8341 tggcatcgac agcagtcgca gtttcttccg caggaattgc tgaaacagca atgctttcaa
     8401 tcgtatctgc caccgccggt acgaatgaaa cactttcgcc taagccgtta ataattgctt
     8461 gttgtaatgc ctcaatcgct tgttgggcgt cttctccctc tgccacaaag cgtaaacgat
     8521 gcccttggct tgcccctagt gctactactt tcattgcact tttcgcactt gccggtgcag
     8581 tgttgcggtc taaattttca acgctgattt ttgaggagaa cggtttcacc gtttgtacta
     8641 acaccgcagc cgggcgggcg tgtaagccgt tgtcgttacg aatggtgaat gtgccaacca
     8701 cttggttgcc actcgcttcg acattggtta caggggttga agctacgctt tcgcctttta
     8761 ataaaccaag catttgctcc gcagagaaat gagcaaactg gcttcttact tctggtttta
     8821 ataattgagt taatagtggc tgtagagcat tgtcggtttc tgccacagtt agtagggttt
     8881 gaccttgcgt attacgggca atagcgatac cgttagcttg gttgcctttg gcggaatcca
     8941 ccacaaatag attattgcct aaatgtaacg ctttgttggc aaccacatcg ctgatgaatg
     9001 cttgggaaac atagccttgc tcttgtagtt ttgcggcgtt aatggctgat aaagtgatag
     9061 ggcttgaaga ttcgatattc agagaaatca gctcttgaga taaggcagga aggtttttct
     9121 taccgcttaa tacggcaata aattcatcta aattttgcgt tttcgctaat gtggcagcag
     9181 cctcttcatc acttaataca gtggtgagtt gacggagtaa ggttaagtgt tcgtccgatt
     9241 tagccgcaat cccgataact acataagcag tgttgccttc gccccattcc acgccttgcg
     9301 gaagttggat aatttgcacg ccggtttctt tgactaaatc acgggtatca agcgtgccgt
     9361 ggggaatagc aattccgtta cctaaaaaag tagaggtttg ggtttcacga ttaagcatac
     9421 cgacttcata gccggctgct acattgccct tggcgactaa ttcggcggca acaaaacgga
     9481 taacttcttc tttgttgctt gctgaaccat ttaaacggat gttttttgcc gataaattaa
     9541 gcattttagc tccttggatt taaagttgat ttttaaactg taatgttatg gttagtttac
     9601 tctatatttt gctaaaccgt tatagccaac taaaaatatc cataaaaatg tttcaaaatg
     9661 tgatctatgt cacaaaattt aaggtttgat gatttaaact aaagattaaa acgattttat
     9721 tttgtgattt agctcaaaaa atggcgtaaa tccacttctt ttatttctat ttagtgatag
     9781 agtgagaact attgaaattt aagtattaaa cgctttaagc aaggagtgcc gatgcaagac
     9841 tatacttatt ctgaaaaaga tgcttttttt gttgagcgac tttcttcggg ttcttgtaat
     9901 gatcctttct cctacctagg tattcaccct agcaataacg gcgttattat tagggtattt
     9961 cttccttctg ccatagcttg ccaagtgatc gatttaaacg gaaagcctgt gtcatcaatg
    10021 ctaaaaattg atgatagagg cttttttatt gcagagctga ttggtaaaaa aacagatttt
    10081 tcttaccgct tgttggtgga ataccctgat tcaacggtgg atatagaaga cccttaccgt
    10141 tttaaaaccc atattcaaga gcttgataat tggttgtttg cggaaggaaa acaacttcgc
    10201 ccgtacgaaa aattaggcgc acatttagtc acacaaaatg aagttggggg tgtgcatttt
    10261 agcgtctggg cgccgaacgc tcagcgggtt tccgtggttg gggatttcaa ccactggaac
    10321 ggcttggtac attcaatgcg attccacccc gattccggta tttgggatat tttcctgccg
    10381 aatgtgaaaa aaggggagtg ctataaattt gaagtgttgg atcgaaacgg cgtgctacgg
    10441 ttgaaatccg acccttacgc ctttgcctcc caattccgcc cagaaaccgc atctattgtt
    10501 tccggcttgc caagcattgt gccagccgag aaaacctgcc gtaatgcgaa tgctcccgat
    10561 aaaccgattt ccatttatga agtgcattta ggctcttggc gacgcaacat tgaaaacaat
    10621 tattggctta gctatgaaga gcttgccgat gaactcattc cttatgtgaa agatatgggc
    10681 tttacccata ttgaattgct gcctatttcc gaatttccgt ttgatggctc ttggggttat
    10741 caaaccaccg gtatttattc gccaaccagc cgttttggta cgcctgaggg cttgagttac
    10801 tttatccaaa aagctcacga taaaggcatt ggtgtgattc ttgactgggt agtggggcat
    10861 tttccggtag atgaacacgg cttggtttat tttgatggaa cgcatttata cgaacattcc
    10921 gatccgaaag agggctacca caaagattgg aataccttaa tttttagcta cggcagaaat
    10981 gaggtgagca attatttgtc tggtaatgca ctttattgga ttgagcgttt tgggattgat
    11041 gcgttgcggg tggatgcggt ggcgtcaatg atttatcgtg attactcacg ttcagacggc
    11101 gagtggattc caaataaata tggcggacgt gagaacttag aggcaatcga cttcttacgc
    11161 cacaccaata atattttgag agacgaaagc caaggcggtg cgagcattgc agaggagtcc
    11221 acttcttttg ccggagtgtc tcactcaact gaaagaggcg gtttgggctt taattataaa
    11281 tggaatatgg gctggatgaa cgacacttta cgctatatgc aaaaagatcc aatttaccgc
    11341 cgttatcacc acaatttaat gacttttggt atgacctacc aatacagcga acagtttgtg
    11401 ttaccgcttt cacacgatga agttgttcac ggcaaaggtt cattgctgaa taaaatgccg
    11461 ggggattgct ggcaaaaatt tgcgaactta cgagcctatt atggctatat gtggggctat
    11521 ccgggcaaaa aattgctctt tatgggcaac gagtttgctc aaggtaaaga gtggaatttt
    11581 gccgagagct tagattggta tttactcggg gaagaagggg gcgaatggca taagggcgta
    11641 ttgggttggg taaaagccct taaccattta tacacccaaa cccctgcact gtttgagcaa
    11701 gattacaatc ctgaaggttt tgagtggtta gtggtggacg atcacgataa ttcggtgttt
    11761 gcttttgagc gtaaagccaa aaatggcgat tcggtgattg tggtgagtaa tttcaccccg
    11821 attgtgcgtg aaaattaccg ttttggggtg aatgaattgg cagaatatca ggaaattctc
    11881 aattctgatt tagcttgtta ttgcggtagt aatgtcagca atgcaaaccg atttgccagc
    11941 gagttaattg catcgcacgg taaagcctac tccttgagca ttactttgcc accgctatcg
    12001 actatcttcc tcaaaagagc cgcagacaaa aaaacaaaac aaaacaaaac ataacttaaa
    12061 gaaacataaa aaataatatg cactatttta gaggtaagcc ttttccttta ggcaataaac
    12121 ttgatagaca agcgggtgaa tttggcacaa attttgccat tgtttcgcaa aatgcaaccg
    12181 ctattgagtt atgtcttttt gatgaaaatc accacgaaac ccgtattccg atgttttctt
    12241 ccggagatac ttggcacatt tttgtagcag gtgtacaagc agggacaaaa tatggctaca
    12301 gggtgaaagg catacaagat gaacaagccg gtttgttatt taatccgcaa aaattattga
    12361 ctgatcctta cgctaaagcg attgaaggga agccggattt aagtagtgca gaggcagccg
    12421 cttggtataa atggaacgat gaacgggata atgcctctct tgcacctaag tctatcgtga
    12481 ttgatgagca ttttgattgg gaaaatgata gccagttatt cactccttgg gcagagacta
    12541 ttatttatga aatgcaggtg aaaggtttta gtcagcggaa ctttcagatt ccaagcgaca
    12601 ttcgaggcac ttttgccggt ttggctcacc ctgtttcgat agactattta aaaaccttag
    12661 gaatcaccgc agtagaactt ctacccgtta cttaccatat tgatgaaacc catttgcaga
    12721 aacaagggct tgtgaattat tggggctata atgtattagg taattttgct attgatccga
    12781 atttagcagc agataagcaa aatcctctta aagagtttaa acagctcgta aaaacattac
    12841 atcaagcaca tatcgaagtg attatggata ttgtgtttaa ccacacagct gagagtgata
    12901 aaaacgggcc gatgctaagt tttagaggca ttgacaatac agcttattgg ctagataaac
    12961 agggcgaata cgaaaattgg actggctgtg gtaatacctt aaatttgagc aatccttata
    13021 tactctgctg ggcaatcgat tgcttaatct attgggtgga ggagtgccat attgatggat
    13081 tccgctttga tcttgcctca attctaggta gaacaccgag tttttctgaa aaagcagatt
    13141 ttttcacagc acttaaaagc cattctaaat taacacatat taagttgatt gctgagcctt
    13201 gggatattgg tgtagaaggt tatcaattag ggcaattccc tgagcctttt tatcagtgga
    13261 atgattgtta tcgtgatgat atacgccgtt tttggttaca aaaccaaggt gattgggggc
    13321 ttttggcacg gcgattggca ggaagtgatg atgtgtttac taaatattcc gtttcaaaaa
    13381 gtatcaattt tattacggca cacgatgggt ttacgttaca agatctcacc agctatcagc
    13441 aaaaacataa ttgggcaaat ggtgaacaaa accgagacgg gcataatagt aactacagca
    13501 ataaccacgg tgttgaaggg cagacagcag atgaattggt aaaaaaacag agagaaatca
    13561 ccgcttgtag tttgcttgct acgctctttt tatcggctgg tgttccaatg cttttagccg
    13621 gtgatgaaat cggacatagt caacaaggta acaataatag ttattgccaa gataatgaga
    13681 tcacttggct agattgggaa aatatgaatc acacacggct ggattatgtt cgtagtttaa
    13741 tccggttgcg taaaaaaatc gctttgcttt ccaagcagaa taattggtgg caagaggatt
    13801 cggtacaatg gttgaatcct tctgctcagc taatgaatat tgacgattgg cataatcaac
    13861 ataatgggct tgtcgtttta ttacaggaag aatggctgct tttattcaat gctaatcgac
    13921 aacctcaacc gtttttactg ccacaaggca actggaaagt tgtgttggag caggctcaag
    13981 gtattttgat gacagacacg caagcggtta cgctcaataa tatgggtgta tgcgtacttc
    14041 aaaaaagtga tgtaatgttc gctaataaat tggagacaca atatgcttaa ccaagaatca
    14101 acggctcttg ttgcaagaca agatttaacc aacgatacct tagtattaat tcttgcagga
    14161 ggacgaggtt ctcgcctata cgaattaacc gataaacgtg caaaaccggc agtctatttc
    14221 ggtggtagcc gccgtattat tgattttgcg ttatcaaatt gtattaactc gaatttatta
    14281 aaaatcgggg tgattaccca atatgcagca cactctttgt tgcgccattt acaacgtggt
    14341 tggtcatttt taccttatga acgtaaccaa tatattgata tgttaccggc acgccaacag
    14401 ctagatgaaa acacttggta tcgtggcaca gcggatgcgg tgtatcaaaa tatggcgatg
    14461 atgaaatcgc actatagacc aaaatatgtg gtgattttag caggggatca tatctataaa
    14521 atggactaca ctcaaatgct gaaagatcac gtcgatcaag gtgcaaaatg tactgtaggt
    14581 tgtatcgaag tgcctcgctc gcaagcaaca gagtttgggg taatggcggt gaatgaaaag
    14641 ctgaaagtga aagagttcgt cgaaaaacca gctgatccac ctgcaatgcc gggaaaaccg
    14701 gattcctcgc tagcatcaat ggggatttat gtatttaatg cagattatct atatgaaatg
    14761 ctggaaaaag aagcctctac accgggaacc tcacatgatt tcggtaaaga tattattcca
    14821 aaagcagtag agcaaggtgt gctatatgcc cacccatttg aacgttcttg taaagggcgt
    14881 aatgccagtg gggcaattta ctggcgagat gtcggtacga ttgacagcta ctgggctgcc
    14941 catatggatt tagtttctga agatccggaa ttagatttat atgatgaatc ttggccgatt
    15001 cacggtcgtc cgcaacaaac tgcaccggca cgttttttct ataatcgtgc gcgtgccaga
    15061 accctagata actcactgat tgcgggtggt tgtgtgatta ccgatgcgga aatcagtaat
    15121 tcggtcttat ttaaccgtgt tcacgttgaa gcggagtcgg tgattgaaca cacagtgatt
    15181 ttaccgcagg tgactatcgg taaaaattgc gtaattaagc gcgctgtgat tgacagacat
    15241 tgtgtaattc ctgatggctt agaaattggt gtaaatgccg aagaagatgc gaaacgtttt
    15301 cgtgtcagca aaaatggcat tgtattagtg acacagtcaa tgttagataa attagcggga
    15361 aaagaagcta attcggatat taaatataca atttagtaag agatgatata atgcttcgag
    15421 tttgtcattt gaggtcgcaa gctcgaagta ttgttatgat taaagcgctt caagcggtct
    15481 tatttttcaa ctattttaca catagaaaac ggttaaaaat gaaagtttta catatttgct
    15541 ctgaaatgta ccctttaatt aaaacaggcg gtttagctga tgtaatgggg gcattgccat
    15601 ttgcgcaaca gcaagctggt aatgatgtca gagtgttaat tccttattac ccgaaagtcg
    15661 cgcaaacttt aggggaaacg gtagaagttg ctactatcgg cacttttgcc ggaacagtct
    15721 ctgttcgatt tgcttatttt aatggcttag gcgtttacgt tatcgatgcg cctcatttat
    15781 ttatgcgtga tggtaaccct taccacgaca gcaattatca agactatccg gataactata
    15841 aacgctttgc tttattaggt tacattgggg cgcaattagc ccaaggtttg gatcattggt
    15901 ggggaaccgc tcaagtttta cacgcacacg attggcaagc agggcttgct tgtgcatatc
    15961 ttaaaagttg gggaagcaat gtaaaaacgc tcttcactat tcataatatt gcctatatcg
    16021 ggcgctttaa tgccttccat ttagcagagc ttggcttacc ttgggaattt ttccacgccg
    16081 aaggcttaga attttacggt gaaatttctt acctcaaagc aggtttgtat tttgctgatc
    16141 gcattactac cgttagcccg acctatgctc gtgaaattac tgaggaaatt gccggtgcag
    16201 gcttgcatgg tttactggca acccgtcaat atcaaggtag attgcgtggc attttaaacg
    16261 gggtcgatgc tgaggtgtgg aatccggaaa cggatacaca tattgtggtg aattatcgtg
    16321 cgaactatat gcacggtaaa gccaaaaata aagcggaact ccaacgaatt tttggtttgc
    16381 cagaagacaa agaagcatta ttgatggtaa tggtgactcg tttaactgag cagaaggggg
    16441 cagatttctt actcgccaat atagatacct taatgcaaca taaggtacag attattgtat
    16501 tgggaagtgg atcgccagat ttagaaaatg cgctacgtta tttccaacac aaatacccga
    16561 accaaattgg ggtgaaaatc ggttatgacg aggctttatc gcaccaaatt attgcaggtg
    16621 gtgatctgat tctagtacca agccgttttg agccgtgcgg attaactcaa ctttatgggc
    16681 tgaaatatgg cactttaccg ctggttcgcc gtaccggtgg cttagctgat acggtggtag
    16741 atagccataa agagagcatt gaaaaccgca gtgccaccgg atttgtattt gattacccag
    16801 atgcacctgc gtttttagag gcagctatgc gggcaatcag cttatggcag aaacaaaaat
    16861 tgtggtcgag cgttcgccag aatgcccttg ctcaagattt tggttgggca aaagtggcag
    16921 aacagtacca aaatttatac cacgaaatta tttaatttgt attcacaaca tcatttcaag
    16981 cggtcgaatt tgcggatttt tttgtaaaaa cgaccgcttg caatattaaa aattaggaga
    17041 atttactcaa tgagcaggca gtcattaagc ccagaaatga tcaaaaatgt taaaaatgcg
    17101 attttgcaca aaatggtgtt cgctttaggg gtcgaacccc gtgatgccag taagcgtaac
    17161 tggctcaatg caaccttacg tgtagtgcgt gatttatcta ccgaagattg gttacaaacc
    17221 cgccgtagcc aggttgcaaa tggtagccgc cgtgtttatt acctttcaat ggaattttta
    17281 atggggcgta cgttcagtaa cgcaatgatt tctgaaggtg tgtatgagct tattggcgct
    17341 gcgttaaaag agttagggca agatctggaa gagattatca atgaagaggg cgtccgggct
    17401 taggtaacgg tggcttaggg cgtttagccg cctgctatat ggacagcctt gccacaatga
    17461 aaattccggc aatcggctac ggtattcaat atgaatacgg tatgttccgc caagaaatcc
    17521 gcaacgggga gcaaattgag caacctgatg aatggttaga gaatgaattt gtgtggccat
    17581 atctacgttc aagtaagcgt ttcccggtac gctttggcgg taaaacttgg cgtgagggca
    17641 aaaaagtagt atggcagccg gaagaagaaa ttaccgctca agctcacgac caactgatcc
    17701 cgggctttga gaccacctca actaacagct tacgtttatg gtctgcacac gcatcgggta
    17761 gagggtttgg cttatctgat tttaaccgtg gcgattattt tgcagcaatg gttaaacaaa
    17821 acagctcgga aaacgtctct cgtgtgcttt atccggacga ttctacctat aacggtcgtg
    17881 aattacgctt acgccaagag tatttccttt gctcggcatc tgtacaagat attatccgcc
    17941 gccacgaagc ggagttcggc tcttgtgtga atttagccga caaagtggca actcacttaa
    18001 atgacacaca cccaaccctt gcggtgcctg agttaatgcg tattttaatt gacgaaaaag
    18061 gctatggctg ggaacaagcg tgggcgatga ctcgtaaaat tttcttctac accaatcaca
    18121 ctttgatgag tgaagcgtta gaaacttggc cggtagaaat gttagcccgt gtgctaccac
    18181 gccatttaga gattattttc gacattaacg aatatttctt agaggaagtg cgtagccaat
    18241 tcccggaaga tcacgatttg atttctcgtg tttccttaat tgatgaacaa ggcgatcgcc
    18301 gtgtgcgtat ggcgtggctt gcggtagtcg cctcagcgaa agtaaatggt gtggcgaaaa
    18361 ttcactcgga tttaatggtt gaatctattt ttgccgattt tgcccgtatt tacccaaatc
    18421 gttttactaa tgtgactaat ggggtgacac ctcgccgctg gatcaaaatt gccaacccag
    18481 ggcttgctaa tattttagat aaatgtatcg gcaataagtg gctgactgat ttaagtgaat
    18541 tggaaaaatt caacgtgttt gttgatgatg ctgatgtgca agcacaggtg gcagcggtga
    18601 aagtagaaaa caaacgtcgc ttagccaaat atgtcgaaga aactcaggga attaaactta
    18661 atcctgaagc gatttttgat gtacaagtta agcgtattca caagtataaa cgccagcaaa
    18721 tgaacgtgtt gcatatcatc acgctttata accgcatctt gaaaaacccg actgctgatt
    18781 ggacacctcg tgtgtttatc ttcgcaggga aagcggcaag tgcttattat gcggcgaaaa
    18841 aagtgattcg tttgattaat gatgtggcaa atgtggtgaa taatgaccct cgcattaatg
    18901 atttaattaa ggtggtgttt attccgaact acggtgtttc attagcacaa atgattattc
    18961 cggcagctga tgtttcagag caaatttcct tagcgggtac agaggcctcc ggaacctcta
    19021 atatgaaatt tgccctcaat ggtgctttaa ccattggtac attagacggt gcgaatgtcg
    19081 aaattttaga tcacgttggt aaagagaaca tctttatttt cggtaatgaa gtagagcagg
    19141 tggaagagct tcgccacaac ggttactcac cgtatcacta ctacgaaaaa gacagcgaat
    19201 tgaatgaggc aatttcacaa attctaaacg gtaaattctc accggaagat ccgtatcgct
    19261 atcaagattt gatcttaaat tcaggtgact actaccaagc ctgtgccgat tatcgtagct
    19321 atgtggaggc tcaagaaaaa gtggctcaag cctatcgtaa caagaaagct tggacacgtt
    19381 cagccatcat caatattgcc aatatgggct atttctcctc agaccgctcg gtgatggatt
    19441 atgcgaaaaa tatttggcat atcgaaccga tgagcgaaga acaattaaaa ggcgattcta
    19501 caatgggcag tattattgaa agttcaaata aactgttgaa tttaaaataa tgatttagag
    19561 aaaacgagta atcttaccgt ttttgtttta gtcagggatt ttgcaaattt ctactaaaaa
    19621 ttaaccgctt gtcaatttgc cttctccacc gccgtgagca ttcccctcag taaattcagc
    19681 tcattagttt ccagttcggc acgttggtat aaacggcgga gttttagcat cactgaatca
    19741 ttgcggataa agcctaattc ggtatataag cgttcggtgt gagcaaagaa atgctctaag
    19801 gcttgagcgg ttgggtaatc cttatctgct gcaagcggtt gtttttcgcc agaattttgc
    19861 aaataagcca ttcggatttc ataacttgcg agctgtactg ccattgccaa attcagagaa
    19921 ccataatcag gattggttgg gaaattcaaa tggtactggc atttcaataa ttcttcgttg
    19981 gttagcccca ctcgctcacg tccaaataca atcgccactt tgccaagatt ggttcgattt
    20041 accactaatt caccacagtc tcttggttca atcagggtat tttgcaaatg gcgaagtctg
    20101 gcactggtgc cgatcaccag ttggcaatcg gcaatggctt catcgaaagt ggcgtagcat
    20161 tgagcgttat ccaacacatc ttttgcccca gcagcaaggg cttcggcttg ttcatcaagc
    20221 ggttgttttg gggcgactaa tcgcaaatgg gttaagccca tcgttttcat tgctcgggct
    20281 gcggaaccga tatttgccgg gagtgaagtt tcaacaagaa tgatctgaat ttggttgaga
    20341 atctgcattg gttttgtttc ttttttgtta aatttagcct gttaatagtg tagcaaaaat
    20401 ttagcctgct taaaatttga aaaaatggga aataatcggt ttaattgggc ataatttttt
    20461 acctatttgt tttatttagt ggtacattta gagcgttttt aatcacaaca accatacagg
    20521 agtctttatg cttattggtg taccaagaga gctgttagac ggtgaaactc gagtggcggc
    20581 aacgccaaaa accgttgagc agatcaaaaa acttggcttt gacgtacttg tggaacaaga
    20641 cgcaggtttt aaagccagtt ttgaagataa tacattttta aatgcaggtg caaatatcgg
    20701 cacacaacaa gaagtttgga atgcagacat tatttttaaa gtgaatgcgc cgacagaagc
    20761 tgaaattgag ttaatcaaag aaggggtaac cttagtcagc tttatttggc cggcacaaaa
    20821 tccacaattg atggaaaaat tgcagtctaa gaaaattaat gtgttggcaa tggacgctgt
    20881 gcctcgtatt tcccgtgccc aagctctcga tgctttaagt tcgatggcaa atatttcggg
    20941 ttaccgtgcg gtgatcgaag cggcaaacgc ttttggtagc ttctttactg ggcaaattac
    21001 cgcagcaggt aaagtaccac cggcaaaagt gttggtaatt ggagccggtg ttgctggctt
    21061 ggctgcaatc ggggcggcaa atagcctggg ggcgattgtg cgtgcctttg actctcgtcc
    21121 tgaagtaaaa gagcaagtta aatcaatggg ggctgatttc ctcgaaattg attttgaaga
    21181 agaaggtggc tcaggtgacg gctatgcgaa agtgatgtcg gaagagttta accgtcgtgc
    21241 aatggcgtta tatgccgagc aagccaaaga ggtggatatt attattacca ccgctgccat
    21301 tccgggcaaa cctgctcctc gtttaatcac taaagagatg gttgattcaa tgaaaccggg
    21361 ttcagtgatt gttgatttag ccgctgcaac cggtggtaac tgtgaataca ccaaagccgg
    21421 cgagattgtg gtgaccgata atcaagtgaa agtgattggc tataccgatt ttcctgcacg
    21481 tttaccaacg caatcttcgc aactttatgg tactaacttg gtgaatttac tcaaactgtt
    21541 agcaccaaat aaagatggtc aaattgagat taattttgat gatgtagtgt tgcgtggcgt
    21601 aacggtggtg cgtgacggcg aattaacttg gccggctccg ccgattcaag tatctgccca
    21661 actgcaaaaa caagcagccg caccacaagc ggtcaaaaaa gaggaaaaac ctgcagaccc
    21721 tcgagtgaaa tatggcgtga tggcagcagc aggtgtgctg ttcctatggc ttgcatctgt
    21781 ggctccggca gcgtttttat cgcattttac cgtattcgtt ctcgcttgtg tggtagggta
    21841 ttatgtggtt tggaacgtca gccacgcact acatacccca ttaatggcgg taacaaatgc
    21901 gatttcaggg attatcatcg taggtgcggt attacaaatt gcccaaccga ccggtaattt
    21961 ctttattgat attttggcat ttattgcgat tttggttgcc agcatcaata tttttggtgg
    22021 ctttaaagta acgcaacgta tgcttgcaat gtttagaaaa ggttaaggag aaacgaaact
    22081 atgtcttttg gatttgtgac tgctgcgtac attattgcag ctattctctt tattatgagc
    22141 ttagccggtc tttctaaaca cgaaaccgcc aaagcaggtt gttggtatgg gattgtgggt
    22201 atggcgattg ctttagtggc aaccattttc ggcccgcaat cacatggcac gatttggatc
    22261 ttaatcgcaa tggcaatcgg tggattcatc ggggtgaaaa aagccctaaa agtagaaatg
    22321 accgaaatgc ctgaattagt ggcaattcta cacagctttg tcggtttagc agcggtattg
    22381 gttggtttta acagctacgg acttcatacc aatgcattaa tgcctgcaaa tttagatgaa
    22441 actgcacaag cggcattttt agcggaacaa gccactcttg ccaatatcca caatgtggaa
    22501 gtgttcttag gtattttcat tggtgcggta accttctccg gttcattggt tgcctttggt
    22561 aaactaagcg gtaaattatt tggtcgtaaa gtctcttctg cagcactgaa tattccacat
    22621 aaacacaaat ggaatttggc agccattatc gtttctgttt ttttgatgat cgtgttttta
    22681 aataatccgc acaatatctt ccctgtgctg attatgacaa ttattgcgtt agtcttcggc
    22741 tggcacttag tttcttctat tggtggggcg gatatgccgg tggtagtttc aatgctgaat
    22801 tcttattcag gttgggcagc cgcagcggca ggctttatgc taagcaacga tttattaatc
    22861 gtaaccggtg cattagtggg atcatcaggt gctatccttt cttatattat gtgtaaagca
    22921 atgaaccgct cattcattag cgtgattgcc ggtggttttg gcaatgaagt tcagtctggc
    22981 aaaggtgatg aagaatacgg tgaacaccgt gaaacgaaag ccgaagaagt ggcagaaatg
    23041 ctgaaaaatg caagctctgt gatcatcacg ccgggttatg gtatggcagt ggctcaagca
    23101 caatatccgg tagcggaaat caccgcaaaa ttgcgtgaaa aaggcgtgaa tgtgcgtttt
    23161 ggtattcacc ctgtggcagg gcgtttaccg gggcatatga acgtattatt ggcggaagcg
    23221 aaagtacctt atgatgtagt gctggagatg gacgaaatca atgacgattt tgaagaaacc
    23281 gatgtggtat tggtaatcgg tgcaaacgat accgtaaacc ctgctgcatt agacgaccca
    23341 tcaagcccta ttgctggaat gccggtgctt gaagtatgga aagcacaaaa tgttatcgtg
    23401 ttcaaacgtt caatggcagt aggctacgcc ggtgtgcaaa acccactgtt ctttaaagaa
    23461 aatacccaaa tgctatttgg tgatgcaaaa gaacgtgttg atgatatttt acgggcgtta
    23521 aattaaattt aaataaaaaa gcgaacataa gttcgctttt ttgtgcttag gctaacataa
    23581 attgttaatt ttttactcta ataccatcaa tccgggaaga gtcgcaaaac tctgcgattt
    23641 tacggtatca tggaaatcat ctaagtaggc tagggtagca tcggcttctc gaatagcgcc
    23701 ataaagggtg ctatatagtc cttgctgagt tatttttctt gccaccacat accccctatc
    23761 taaatatggt ttaaccgccc aataaggtag tgcagcaatg cctcgccgac ttgctactag
    23821 ctgaataata gcaatagtta actcactatt acgccgagta actttgatgc cagcagggcc
    23881 taatattttt cgcagtaaat ctagcatatc atcaggtact ggataggtaa ttaatgtctc
    23941 atctttaaaa tcttcagctt cccatacatc ttttgtggca agagggtgat ctttagagca
    24001 aatagctacc atttcatatg aaaagagagg tttgtatata ataccagcac tatcttcctc
    24061 ttcagcgaca attgccagat ctgcacgatg gcttaatagc aaaccaaccg tatcagtatg
    24121 gaaacccgat acgatatcta attcaactag aggccaatgt tgacggaaag tatccattgc
    24181 cggcattagc caatcaaaac aggtatggca ttctactgca acacgtacct ctccgacttc
    24241 tccttgtttc actcgggcta aatctaattc agcctcaatg actttgggca aaatatcgta
    24301 ggccaactta attaaacgtt cgccagctgg tgtaaagcga ataggggagc tttttcgctc
    24361 aaatagcgta agatcatact gttcttccag taattttatt tggtgtgaaa gtgcggattg
    24421 tgttaaatgg acacgctttg ctgcaagaga tacgctaccg ctctctttta atgcgattaa
    24481 tgtccgcaaa tggcggattt caagaaaaat cggtttcata atttattcat ctaaaagtga
    24541 aaaaagatga aaataattat aacaaacaag cggtcaaatt ctgcaaaaaa tttgcaaaat
    24601 ccaaccgctt gtatagagca gtggcgaatt attgggttaa ttgaactaaa atgcggcgaa
    24661 caggctctgc tgcaccccat aataattggt cacctacggt aaatgcagct aagtattctc
    24721 cacccatcgc taatttacgc aagcgaccaa ccggcacgct caatgtaccg gtcactttcg
    24781 ccggggtcaa ttcacgcaat gtggtttctt tgtcgtttgg aattactttc acccattcat
    24841 tgtgggcagc aataatttgc tcgatttccg ctaacggaat atcttttttc agcttaattg
    24901 tgaatgcttg gctgtggcag cgtaatgctc caatacgcac gcataaacca tcaactggaa
    24961 ttgggttatc gcttaaacct aaaattttat tagtttctgc ataacctttc cactcttctt
    25021 tagtttgccc ggtttccggt aataatttat caatccaagg aattaagcta ccgcccaatg
    25081 ccgcaccgaa gttctcggtt gggaagtctt ccgcacgcat tttagccgta actttacgtt
    25141 caatatctaa aatagaagag gcaggatttg ctaattcagc ttttacgcta tcttctaatt
    25201 cacccatttg agaaagcaat tcacgcatat ttttcgcacc ggcaccggaa gcggcttggt
    25261 aagttgctac agaaacccac tcgaccaaat ctttctcaaa taaaccaccg atagccatta
    25321 gcattaggct tacggtacag tttccgccta caaatgtttt aatgccgttt tttaagcctt
    25381 ctgaaatcac atgttggttc accgggtcta atacgataat agcatcgtct ttcatacgta
    25441 atgcagaagc cgcgtcaatc caatagccgt tccaaccggt ttcttttaat tttggataga
    25501 cttcatttgt ataatcacca ccttggcaag ttacgataat gtctaatttt tttaattctt
    25561 caatgtcaaa ggcgtttttt aattcgccgg catcttttcc gccaaaaaca ggggcttttt
    25621 gacccgcttg tgaagtagtg aaaaaaacag ggttgatatt ggcgaagtta ttttcttcaa
    25681 ccatacgatc cattaagacc gaaccgacca taccacgcca accgataaaa cctacatttt
    25741 gcattgtgtt tgctcctaaa aatgatgtta taaagattgt gaataaatac aggattttag
    25801 tgcaaaaatc aatgtttttt attgatttgc aaagttttga ca
//
LOCUS       DANLXS010000012        25333 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.012, whole genome shotgun sequence.
ACCESSION   DANLXS010000012 DANLXS010000000
VERSION     DANLXS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25333
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.012"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            131..634
                     /locus_tag="PXD71_000403"
     CDS             131..634
                     /locus_tag="PXD71_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HDL6290563.1"
                     /translation="MNQSYFNLLGNITWLWMNSSLHKEWSCELLARNVIPAIENEQYM
                     LLIDNGIPIAYCSWADLNLETEVKYIKDINSLTPEEWQSGDRRWIIDWVAPFGHSQLL
                     YKKMCQKYPDMIVRSIRFYPKQKELGKIAYFKGGKLDKKTAKKRFDTYQEELATALKN
                     EFNFIKK"
     gene            650..3511
                     /locus_tag="PXD71_000404"
     CDS             650..3511
                     /locus_tag="PXD71_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX family hemolysin"
                     /protein_id="HDL6290564.1"
                     /translation="MGTRLTTLSNGLKNTLTATKSGLHKAGQSLTQAGSSLKTGAKKI
                     ILYIPQNYQYDTEQGNGLQDLVKAAEELGIEVQREERNNIATAQTSLGTIQTAIGLTE
                     RGIVLSAPQIDKLLQKTKAGQALGSAESIVQNANKAKTVLSGIQSILGSVLAGMDLDE
                     ALQNNSNQHALAKAGLELTNSLIENIANSVKTLDEFGEQISQFGSKLQNIKGLGTLGD
                     KLKNIGGLDKAGLGLDVISGLLSGATAALVLADKNASTAKKVGAGFELANQVVGNITK
                     AVSSYILAQRVAAGLSSTGPVAALIASTVSLAISPLAFAGIADKFNHAKSLESYAERF
                     KKLGYDGDNLLAEYQRGTGTIDASVTAINTALAAIAGGVSAAAAGSVIASPIALLVSG
                     ITGVISTILQYSKQAMFEHVANKIHNKIVEWEKNNHGKNYFENGYDARYLANLQDNMK
                     FLLNLNKELQAERVIAITQQQWDNNIGDLAGISRLGEKVLSGKAYVDAFEEGKHIKAD
                     KLVQLDSANGIIDVSNSGKAKTQHILFRTPLLTPGTEHRERVQTGKYEYITKLNINRV
                     DSWKITDGAASSTFDLTNVVQRIGIELDNAGNVTKTKETKIIAKLGEGDDNVFVGSGT
                     TEIDGGEGYDRVHYSRGNYGALTIDATKETEQGSYTVNRFVETGKALHEVTSTHTALV
                     GNREEKIEYRHSNNQHHAGYYTKDTLKAVEEIIGTSHNDIFKGSKFNDAFNGGDGVDT
                     IDGNDGNDRLFGGKGDDILDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRKGDGNDIIT
                     DSDGNDKLSFSDSNLKDLTFEKVKHNLVITNSKKEKVTIQNWFREADFAKEVPNYKAT
                     KDEKIEEIIGQNGERITSKQVDDLIAKGNGKITQDELSKVVDNYELLKHSKNVTNSLD
                     KLISSVSAFTSSNDSRNVLVAPTSMLDQSLSSLQFARAA"
     gene            3586..5712
                     /locus_tag="PXD71_000405"
     CDS             3586..5712
                     /locus_tag="PXD71_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDL6290565.1"
                     /translation="MEANHQRNDLGLVALTMLAQYHNISLNPEEIKHKFDLDGKGLSL
                     TAWLLAAKSLALKAKHIKKEISRLHLVNLPALVWQDNGKHFLLVKVDTDNNRYLTYNL
                     EQDAPQILSQDEFEACYQGQLILVTSRASVVGQLAKFDFTWFIPAVIKYRKIFLETLI
                     VSIFLQIFALITPLFFQVVMDKVLVHRGFSTLNIITVALAIVIIFEIVLSGLRTYVFS
                     HSTSRIDVELGAKLFRHLLSLPISYFENRRVGDTVARVRELDQIRNFLTGQALTSVLD
                     LLFSFIFFAVMWYYSPKLTLVILGSLPCYILWSIFISPILRRRLDEKFARSADNQAFL
                     VESVTAINMIKAMAVAPQMTDTWDKQLASYVSSSFRVTVLATIGQQGVQLIQKTVMVI
                     NLWLGAHLVISGDLSIGQLIAFNMLSGQVIAPVIRLAQLWQDFQQVGISVTRLGDVLN
                     SPTEQYQGKLSLPEIKGDISFKNIRFRYKPDAPTILNNVNLEIRQGEVIGIVGRSGSG
                     KSTLTKLLQRFYIPENGQVLIDGHDLALADPNWLRRQIGVVLQDNVLLNRSIRENIAL
                     SDPGMPMERVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNN
                     PKILIFDEATSALDYESEHIIMQNMQKICQGRTVILIAHRLSTVKNADRIIVMEKGEI
                     VEQGKHHELLQNSNGLYSYLHQLQLN"
     gene            5724..7160
                     /locus_tag="PXD71_000406"
     CDS             5724..7160
                     /locus_tag="PXD71_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_140441352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDL6290566.1"
                     /translation="MKIWLSGIYEFFLRYKNIWAEVWKIRKELDHPNRKKDESEFLPA
                     HLELIETPVSKKPRLIAYLIMLFLVVAIVLASVSKVEIVATAPGKLTFSGRSKEIKPI
                     ENAIVQEIFVKDGQFVEKGQLLVSLTALGSDADIKKTMASLSLAKLENYRYQTLLTAI
                     EKESLPVIDLSRTEFKDSSEEDRLRIKHLIEEQYTTWQKQKTQKTLAYKRKEAEKQTI
                     FAYVRKYEGATRIEQEKLKDFKALYKQKSLSKHELLAQENKLIEAQNELAVYRSKLNE
                     LENDLLNVKEELELITQFFKSDVLEKLKQHIENERQLRLELEKNNQRRQASMIRAPVS
                     GTVQQLKIHTIGGVVTTAETLMIIVPEDDVLEATALVPNKDIGFVAAGQEVIIKVETF
                     PYTRYGYLTGRIKHISPDAIEQPNVGLVFNATIAIDRKNLTSPDGRKIDLSSGMTITA
                     EIKTGERSVMSYLLSPLEESVTESLRER"
     gene            7401..8399
                     /locus_tag="PXD71_000407"
                     /pseudo
     CDS             7401..8399
                     /locus_tag="PXD71_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            8416..9159
                     /locus_tag="PXD71_000408"
     CDS             8416..9159
                     /locus_tag="PXD71_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6290567.1"
                     /translation="MKQKYIYGLAGLLLLLLGWQLGTALLSQRLPMASMLAPAKTWES
                     FTWLISSGEIFPHLWASLQRIAVGLLAALVIGVPLGLLLGFSKHLEMLCTPSFQLLRM
                     ISPLSWMPIIVMLLGVGDLPIYFLLAFATVWSLIISTTSGVKSIDPQWLQLGRVMSAT
                     RKELLCKIVVPAITGHVLTGLRVAVGIAWVVLVPCEMLGVNQGLGYFILDTRDRLAYS
                     ELMAVIIIIGLIGWALDSLIRLALAKGGR"
     gene            9314..10372
                     /locus_tag="PXD71_000409"
     CDS             9314..10372
                     /locus_tag="PXD71_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL6290568.1"
                     /translation="MSVLSSSFIQWLQQNAENLDQSNERADLLIPQIAALGLYKIGVP
                     ETLGGSGGTLVDAIKAVTELGNHSLAAAFVSWGHRTFIEHIIASKNPIPRQSYLADLL
                     NGNLTAGTGLSNAMKFLSGIEELNVNIREENGKRYLNGRLPWVTNLRSYRFVASFVAG
                     FQDERKPLVIAIPSTAKGLTRTKELEFIALQGTNTTSLVFENVELQDEWILADDAPEF
                     LAATRPAFLGLQFGLPFGLVERSLAEIEKSLPFRAILASEWQAQVDALNDIKTRLFNG
                     LNEEGYFVRNPKELFQARIDIVNVVAQTILLELQASGGRGYLKNDTSGFSRRWKEAAF
                     LPVVTPSAVQLRMVLANS"
     gene            complement(10493..11035)
                     /locus_tag="PXD71_000410"
     CDS             complement(10493..11035)
                     /locus_tag="PXD71_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL6290569.1"
                     /translation="MSQFQIHTIESAPETAQEALKAVQQANGFIPNLIGVLANAPTAL
                     ETYRTVGGINSRNSLTAEEREVVQITAAVVNGCGFCVAGHTKIALKALKMPEEVVNAL
                     RATARIDDNKLDTLARFTLAVMLQKAKLTEAQLNEFFAAGYNQQNAIDVILGVSLATL
                     CNYVNNIAEIPINPELQPFA"
     gene            complement(11187..12698)
                     /gene="glpK"
                     /locus_tag="PXD71_000411"
     CDS             complement(11187..12698)
                     /gene="glpK"
                     /locus_tag="PXD71_000411"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL6290570.1"
                     /translation="MEKQYIIALDQGTTSSRAVLLDKNANVVEVAQREFTQIYPQAGW
                     VEHNPMEIWSSQSSTLNEVVAKAGIKPDSIAAIGITNQRETTIVWEKETGKPVYNAIV
                     WQCRRTSDITDKLKADGHEEYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAERGEL
                     LFGTVDTWLVWKLTQGRVHVTDYTNASRTMLFNIHTKQWDDKMLELLNIPRSMLPEVK
                     NSSEVYGQTNIGGQGGVRIPVAGIAGDQQAALYGHLCVKEGQAKNTYGTGCFMLMHTG
                     NTAIQSENGLLTTIACNAKGEPEYALEGSVFIAGASIQWLRDELKIVHDSFDSEYFAT
                     KEDDSNGVYVVPAFTGLGAPYWDPYARGSIFGLTRGANRNHIVRATLESIAYQTRDVL
                     EAMQSDSKAKLQALRVDGGATANNFLMQFQADILDTKVERPKVKEVTALGAAYLAGIA
                     VGFWKDLEELKDKAEIERTFTPDGDQEKRAKRYKGWKKAVRRSLEWAKEDAEE"
     gene            complement(12702..13487)
                     /locus_tag="PXD71_000412"
     CDS             complement(12702..13487)
                     /locus_tag="PXD71_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL6290571.1"
                     /translation="MSKSLRNACIGEFIGTGFILFFGAGCVAAAQVAGANFGLWEISI
                     VWGLGVSMAIYISAGISGAHLNPAVTIALAAFYGFEKHKILPYVIAQVAGAFCSVALI
                     YFMYSDLFTAAEAAQGITRGETVGFAGVFSTYPNPNITLVTAFIVEFVITVVLMSTIL
                     AIGDDKNGLPNKALAALLIGLLIAVIGGATGPLTGFAMNPARDFGPKLFAYLAGWGEI
                     ALTGGKEIPYFIIPIVAPICGALFGAWGYKNLIHNNLPANTQE"
     gene            13700..14059
                     /locus_tag="PXD71_000413"
     CDS             13700..14059
                     /locus_tag="PXD71_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDL6290572.1"
                     /translation="MISIGAVLGAMCRWQLAVWLNPILSQFAFGTLAANWLGCLLIGI
                     AMGFNLGESQRLLFISGFLGSFTTFSSFSLELTEKLLAEKWGQFVTVLSLHLIGGLLL
                     TLIGVMLARAVTSGRFC"
     gene            complement(14107..15429)
                     /locus_tag="PXD71_000414"
     CDS             complement(14107..15429)
                     /locus_tag="PXD71_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL6290573.1"
                     /translation="MSAMFIIQFAIVLLCILVGARVGGIGLGVFGGLGLAILSFGFGL
                     KPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIATRILRKNPKYITFIAPLVTWTF
                     TVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
                     LEPQGVTLGNVLSVTIPATLLGLGLACVFVNKMGKELKDDPNYQRLLQDPEYVKENHI
                     TTNPTELPLKPTAKLSVGLFLFGALLVVLMGAIPALRPVFDGKPMGMAHTIEIVMLSI
                     GALIIFFCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQGHMDEVKSMVSGLVE
                     TAPWAFAFALFVLSVLVNSQGATVATLFPVAIAIGIPAPVLIGVFVAVNGYFFIPNYG
                     PIIASIDFDTTGTTRIGKYIFNHSFMLPGLLSMIFSIGFGLLLSNILL"
     gene            15578..16402
                     /gene="dapF"
                     /locus_tag="PXD71_000415"
     CDS             15578..16402
                     /gene="dapF"
                     /locus_tag="PXD71_000415"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL6290574.1"
                     /translation="MQFSKMHGLGNDFMVVDGVTQNVYLTEEMIRTLSDRHRGIGFDQ
                     LLLVEPPYDPELDFHYRIFNADGSEVSQCGNGARCFARFVTLKGLTNKKDIHVSTAKG
                     KMVLSLQEDGKVRVNMGEPIWEPSQVPFTANKFEKNYILRTELQTVLCGVVSMGNPHC
                     VLQVDDVKTAPVNELGPLLESHERFPERANISFMQVVNRNHVKLRVYERGAGETQACG
                     SGACGTVAVGIMQGLLDNKVQVDLPGGTLIIEWQGVGHPLYMTGDATHIYDGFIKL"
     gene            complement(16573..17115)
                     /locus_tag="PXD71_000416"
     CDS             complement(16573..17115)
                     /locus_tag="PXD71_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDL6290575.1"
                     /translation="MAQVYIVHGYTANADKHWFPWLEQELAKCGVSCERLNMPDSAKP
                     SLDGWLNHLAQNVELTDQTVFVGHSLGCIAILNFLAKNYAKIKGAVFVSGFYQPVENL
                     PELSAFTNYYAILPSVSSFPSYVVSALDDKVVNHQYSDALAQHLHADYIRLNQGGHFL
                     DREGVTELPVVLELVKKLLE"
     gene            17239..17562
                     /locus_tag="PXD71_000417"
     CDS             17239..17562
                     /locus_tag="PXD71_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HDL6290576.1"
                     /translation="MNVWILLAISICLEIAATNLLKLSNGFTKAIPTIGSLALYGLSF
                     YFLSIIFRTLPVGIVYAIWSGVGIVLTALVAYFAFGQKIDLAGLIGISLIIAGVLVIN
                     LFSKV"
     gene            complement(17625..19070)
                     /gene="xylB"
                     /locus_tag="PXD71_000418"
     CDS             complement(17625..19070)
                     /gene="xylB"
                     /locus_tag="PXD71_000418"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDL6290577.1"
                     /translation="MYIGIDLGTSGVKVVLLDENQHIIAITQKSLPIFRPHPLWSEQN
                     PQDWWNATNEAMLELSATQDLQAVKAIGLTGQMHGATLLDNADNVLSPAILWNDGRSF
                     MECEELEQLVPNSREITGNLMMPGFTAPKLRWVDKHQPDIAEKVSKVLLPKDYLRLLM
                     SGEYASDMSDASGTMWLDVGKRNWNKSLLNACGLDIENMPKLFEGNQITGYLRPTLAE
                     QWKMKSVPIVAGGGDNAAGAIGIGLYQTGQAMLSLGTSGVYFVVSDKFQANPQKAVHS
                     FCHALPDRWHLMSVILSAASAVDWAVKSLGLKDVSTLFQQVEASQTASDAIFLPYLSG
                     ERTPHNDPYAKGVFWGLSHNDNQITMAKAVIEGVSFALAEGIEVLHETGVVADNIALI
                     GGGAKSVYWRQLLADISGKTFEYRTSGDVGPALGAAKLAQIALNPNQDIATFCQPLPL
                     EQVYQPTPARYAEYQQKRQKFTELYRRLKGL"
     gene            complement(19223..20542)
                     /gene="xylA"
                     /locus_tag="PXD71_000419"
     CDS             complement(19223..20542)
                     /gene="xylA"
                     /locus_tag="PXD71_000419"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDL6290578.1"
                     /translation="MSNYFDKISKVQYEGANSTNPFAFKHYNPDEVVLGKTMAEHLRL
                     AVCYWHTFCWTGNDMFGVGSLDRSWQKTGDLLQGAKQKAEIAFEFFQKLGIPYYCFHD
                     VDVAPEGNNFKEYQHNFNTIVDILEQKQAETGVKLLWGTANCFTNPRYMSGASTNPNP
                     EVFAWAAAQVFTAMNATKRLGGENYVLWGGREGYETLLNTDLKREREQIGRFMQLVVE
                     HKHKIGFNGTLLIEPKPQEPTKHQYDYDVATVYGFLKQFGLEKEIKVNIEANHATLAG
                     HTFQHEIATATALDIFGSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKAGGFTTG
                     GFNFDAKIRRQSTDPYDLFHAHIGAIDVLALSLKRAAKMLEDQALQKVVDNRYAGWNQ
                     DLGLQILNGKASLEDLAKIVETQGLDPKPVSGQQEYLENLVNSYIYR"
     gene            20784..21785
                     /gene="xylF"
                     /locus_tag="PXD71_000420"
     CDS             20784..21785
                     /gene="xylF"
                     /locus_tag="PXD71_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6290579.1"
                     /translation="MKLKSKLLAIATATLMVLSNASVAKDLKIGMSIDDLRLERWQKD
                     RDIFVNKAESLGAKVFVQSANGDATAQISQIENMLNKDIDVLVIIPFNGEVLSNVIAE
                     AKKEGVKVLAYDRLINNADVDFYVSFDNEKVGELQAKSIIGQKPEGNYFLMGGSPVDN
                     NAKLFRKGQMKVLQPLIDSGKIKVVGDQWVDSWLAEKALQIMENALTANKNNIDAVVA
                     SNDATAGGAIQALSAQGLSSKVAISGQDADLAAIKRIIAGTQTMTVYKPITNLADKAA
                     EIAVALGKDEKVEMNAQLNNGMKEVPAYLLEPVVVTKENIDDTVIKDGFHTKDAVYK"
     gene            21852..23366
                     /gene="xylG"
                     /locus_tag="PXD71_000421"
     CDS             21852..23366
                     /gene="xylG"
                     /locus_tag="PXD71_000421"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDL6290580.1"
                     /translation="MAKLLEMKNITKKFGEVVALNNISISLETGEILSLCGENGSGKS
                     TLMKVLCGIYPAGDYEGEIYFSGEKLIARNIKDTEEKGISIIHQELTLVKNMSILENM
                     FLGNEITKSGITDDNEMYLRCKTLLEQVQLDIDPNTKVGELGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEKETEILLSLIKDLQAHNIACVYISHKLNEVKAISDKICVIRDG
                     EHIGTRSAEGMSEDDIITMMVGREITSLYPHEPHNIGEEVLRVENLTAWHPTNTHIKR
                     VDNANFVLRQGEILGIAGLVGSGRTEMAQCIFGSYQGKYQGDIFLHKQPIKIKNCAQA
                     IENHIVMVPEDRKKHGIIPIMGVGKNITLSSLPQFCFGKKVINEPLEETIINQSIAKL
                     KVKTSSPELAIGRLSGGNQQKAILAKCLLLNPKILILDEPTRGIDVGAKYEIYKLINQ
                     LAKEGMAIIVISSELPEVLGISDRVLVMHQGKIKADLINQDLTQEKVMEAALKE"
     gene            23370..24497
                     /locus_tag="PXD71_000422"
     CDS             23370..24497
                     /locus_tag="PXD71_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL6290581.1"
                     /translation="MNKLKSINLQVYIMLIAIVVIMSFFSIATDGAYLSARNISNLLR
                     QTSITGILAIGMVFVIISAEIDLSVGSLMGLLGGFAAISNVWWGWPLSLTIIVTLILG
                     VMLGAWSGWWVAYQKVPSFIVTLAGMLAFRGVLVGLTNGTTVSPISNEMTTIGQGYLP
                     DMVGMILGTIGIFCFIVWGSYQRKARQKLNLTVPSMSKDTLKYGLVAIIVLGAIYLLN
                     DYRGVPFPVLVLVILAIAGTFLARKTAFGRHIYAIGGNIDAARLSGISVEKVKLAIFA
                     LNGLLVAIAGLILSSRLGAGAPSAGQNAELDAIAACVIGGASLAGGIGSIYGVVIGAF
                     IIALLDNGMSMLDVPTFWQYIVKGGILLLAVWVDTISKKKA"
     gene            complement(24513..25043)
                     /locus_tag="PXD71_000423"
     CDS             complement(24513..25043)
                     /locus_tag="PXD71_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6290582.1"
                     /translation="MSENSLKTHYSAKELAELSLACLPKTKQGVLYQAKKQGWTTHKK
                     VGVGGEILEFALSNLPQEIQTEIRSRFMSAVVEAKPKKLPAVRAEVDVGNLTTKQREI
                     ADARMALVAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLVELVAVANAKKTAKRTIV
                     PIFSPIMPDFPLDSRD"
     gene            complement(25059..25322)
                     /locus_tag="PXD71_000424"
     CDS             complement(25059..25322)
                     /locus_tag="PXD71_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6290583.1"
                     /translation="MSTLEDIKKTAKDWHRADIIAALHKAGWSLRQLSLKHGYSQSST
                     LKNAVDRPWLKGERIIAEAIGISPEEIWPERYAQRNHKKFADR"
BASE COUNT         7538 a         5084 c         5423 g         7288 t
ORIGIN      
        1 aagacaaaaa atacaattta agctacaaaa aacaacaaaa aacaacaaaa aacacgacaa
       61 taagatcgag taatgattat attatgttat aatttttgac ctaatttaga ataattatcg
      121 agtgcaaatt atgaatcaat cttattttaa cttactagga aacattactt ggctatggat
      181 gaactcctcc ctccacaaag aatggagctg tgaactacta gcacgcaatg tgattcctgc
      241 aattgaaaat gaacaatata tgctacttat agataacggt attccgatcg cttattgtag
      301 ttgggcagat ttaaaccttg agactgaggt gaaatatatt aaggatatta attcgttaac
      361 accagaagaa tggcagtctg gtgacagacg ctggattatt gattgggtag caccattcgg
      421 acattctcaa ttactttata aaaaaatgtg tcagaaatac cctgatatga tcgtcagatc
      481 tatacgcttt tatccaaagc agaaagaatt aggcaaaatt gcctacttta aaggaggtaa
      541 attagataaa aaaacagcaa aaaaacgttt tgatacatat caagaagagc tggcaacagc
      601 acttaaaaat gaatttaatt ttattaaaaa atagaaggag acatccctta tgggaactag
      661 acttacaacc ctatcaaatg ggctaaaaaa cactttaacg gcaaccaaaa gtggcttaca
      721 taaagccggt caatcattaa cccaagccgg cagttcttta aaaactgggg caaaaaaaat
      781 tatcctctat attccccaaa attaccaata tgatactgaa caaggtaatg gtttacagga
      841 tttagtcaaa gcggccgaag agttggggat tgaggtacaa agagaagaac gcaataatat
      901 tgcaacagct caaaccagtt taggcacgat tcaaaccgct attggcttaa ctgagcgtgg
      961 cattgtgtta tccgctccac aaattgataa attgctacag aaaactaaag caggccaagc
     1021 attaggttct gccgaaagca ttgtacaaaa tgcaaataaa gccaaaactg tattatctgg
     1081 cattcaatct attttaggct cagtattggc tggaatggat ttagatgagg ccttacagaa
     1141 taacagcaac caacatgctc ttgctaaagc tggcttggag ctaacaaatt cattaattga
     1201 aaatattgct aattcagtaa aaacacttga cgaatttggt gagcaaatta gtcaatttgg
     1261 ttcaaaacta caaaatatca aaggcttagg gactttagga gacaaactca aaaatatcgg
     1321 tggacttgat aaagctggcc ttggtttaga tgttatctca gggctattat cgggcgcaac
     1381 agctgcactt gtacttgcag ataaaaatgc ttcaacagct aaaaaagtgg gtgcgggttt
     1441 tgaattggca aaccaagttg ttggtaatat taccaaagcc gtttcttctt acattttagc
     1501 ccaacgtgtt gcagcaggtt tatcttcaac tgggcctgtg gctgctttaa ttgcttctac
     1561 tgtttctctt gcgattagcc cattagcatt tgccggtatt gccgataaat ttaatcatgc
     1621 aaaaagttta gagagttatg ccgaacgctt taaaaaatta ggctatgacg gagataattt
     1681 attagcagaa tatcagcggg gaacagggac tattgatgca tcggttactg caattaatac
     1741 cgcattggcc gctattgctg gtggtgtgtc tgctgctgca gccggctcgg ttattgcttc
     1801 accgattgcc ttattagtat ctgggattac cggtgtaatt tctacgattc tgcaatattc
     1861 taaacaagca atgtttgagc acgttgcaaa taaaattcat aacaaaattg tagaatggga
     1921 aaaaaataat cacggtaaga actactttga aaatggttac gatgcccgtt atcttgcgaa
     1981 tttacaagat aatatgaaat tcttactgaa cttaaacaaa gagttacagg cagaacgtgt
     2041 catcgctatt actcagcagc aatgggataa caacattggt gatttagctg gtattagccg
     2101 tttaggtgaa aaagtcctta gtggtaaagc ctatgtggat gcgtttgaag aaggcaaaca
     2161 cattaaagcc gataaattag tacagttgga ttcggcaaac ggtattattg atgtgagtaa
     2221 ttcgggtaaa gcgaaaactc agcatatctt attcagaacg ccattattga cgccgggaac
     2281 agagcatcgt gaacgcgtac aaacaggtaa atatgaatat attaccaagc tcaatattaa
     2341 ccgtgtagat agctggaaaa ttacagatgg tgcagcaagt tctacctttg atttaactaa
     2401 cgttgttcag cgtattggta ttgaattaga caatgctgga aatgtaacta aaaccaaaga
     2461 aacaaaaatt attgccaaac ttggtgaagg tgatgacaac gtatttgttg gttctggtac
     2521 gacggaaatt gatggcggtg aaggttacga ccgagttcac tatagccgtg gaaactatgg
     2581 tgctttaact attgatgcaa ccaaagagac cgagcaaggt agttataccg taaatcgttt
     2641 cgtagaaacc ggtaaagcac tacacgaagt gacttcaacc cataccgcat tagtgggcaa
     2701 ccgtgaagaa aaaatagaat atcgtcatag caataaccag caccatgccg gttattacac
     2761 caaagatacc ttgaaagctg ttgaagaaat tatcggtaca tcacataacg atatctttaa
     2821 aggtagtaag ttcaatgatg cctttaacgg tggtgatggt gtcgatacta ttgacggtaa
     2881 cgacggcaat gaccgcttat ttggtggtaa aggcgatgat attctcgatg gtggaaatgg
     2941 tgatgatttt atcgatggcg gtaaaggcaa cgacctatta cacggtggca agggcgatga
     3001 tattttcgtt caccgtaaag gcgatggtaa tgatattatt accgattctg acggcaatga
     3061 taaattatca ttctctgatt cgaacttaaa agatttaaca tttgaaaaag ttaaacataa
     3121 tcttgtcatc acgaatagca aaaaagagaa agtgaccatt caaaactggt tccgagaggc
     3181 tgattttgct aaagaagtgc ctaattataa agcaactaaa gatgagaaaa tcgaagaaat
     3241 catcggtcaa aatggcgagc ggatcacctc aaagcaagtt gatgatctta tcgcaaaagg
     3301 taacggcaaa attacccaag atgagctatc aaaagttgtt gataactatg aattgctcaa
     3361 acatagcaaa aatgtgacaa acagcttaga taagttaatc tcatctgtaa gtgcatttac
     3421 ctcgtctaat gattcgagaa atgtattagt ggctccaact tcaatgttgg atcaaagttt
     3481 atcttctctt caatttgcta gagcagctta atttttaatg attggcaact ctatattgtt
     3541 tcacacatta tagagttgcc gttttatttt ataaaaggag acaatatgga agctaaccat
     3601 caaaggaatg atcttggttt agttgccctc actatgttgg cacaatacca taatatttcg
     3661 cttaatccgg aagaaataaa acataaattt gatcttgacg gaaaagggct ttctttaact
     3721 gcttggcttt tagctgcaaa atcgttagcg ttgaaagcga aacacattaa aaaagagatt
     3781 tcccgcttac acttggtgaa tttaccggca ttagtttggc aagataacgg taaacatttt
     3841 ttattggtaa aagtggatac cgataataac cgctatttaa cttacaattt ggaacaagat
     3901 gctccacaaa ttctgtcaca agacgaattt gaagcctgct atcaagggca gttaattttg
     3961 gtcacgtcca gagcttccgt agtaggtcaa ttagcaaagt tcgatttcac ctggtttatt
     4021 ccggcggtga tcaaataccg aaaaatcttt ctagaaacct tgattgtttc gatctttttg
     4081 caaatttttg ccctaattac accgctattc ttccaagttg ttatggataa agtactggtg
     4141 catcgaggtt tttcaacctt gaatatcatt acggttgcct tagctattgt gatcatcttt
     4201 gaaattgtac taagtggttt gagaacctat gttttttctc atagcactag ccgtattgat
     4261 gttgaattag gcgctaaatt atttcgacat ttattatcac tacccatttc ttattttgaa
     4321 aacagacgag ttggagatac agtcgctagg gttagagaat tagatcaaat tcgtaatttc
     4381 cttaccggac aagcattaac ctcggtgtta gatctcttat tctcttttat cttttttgcc
     4441 gtaatgtggt attacagccc aaaattaacc ttggtaattc ttggttcatt gccctgctat
     4501 attttatggt caatttttat tagtccgatt ttaagacggc gtttagatga gaaatttgcc
     4561 cgaagtgctg ataaccaagc attcttagtt gagtcggtaa cagccatcaa tatgattaaa
     4621 gcgatggcgg ttgctccaca aatgacggat acatgggata aacagctggc aagctatgtt
     4681 tcatcaagtt tccgtgtcac cgtattagca accattgggc aacaaggtgt acaacttatt
     4741 caaaaaaccg ttatggtgat taacctttgg ttaggggcac acttagttat ttcaggcgat
     4801 ctgagtattg ggcaattaat tgcctttaat atgctatcag ggcaagtgat tgcaccggtg
     4861 attcggctgg ctcagctctg gcaagatttc caacaagttg ggatttccgt cactcgctta
     4921 ggtgatgttt taaactctcc aaccgaacaa tatcaaggca aattatcact accagaaata
     4981 aaaggcgata tctcatttaa aaatatccgc tttagatata aaccagatgc accaactatt
     5041 ttaaataatg tgaatttaga aattaggcaa ggagaagtga ttgggattgt tggacgttcc
     5101 ggttcaggca aaagtactct gactaaatta ctgcaacgtt tttatattcc tgaaaatggg
     5161 caggttttga ttgatggaca tgatctagcc ttagctgatc caaactggct acgccgtcaa
     5221 ataggtgtag tgctgcaaga taatgtgtta ttaaaccgca gtatccgaga aaatattgcg
     5281 ctatcagatc caggaatgcc aatggagcga gtaatttatg cagcaaaatt agcaggggct
     5341 cacgatttta tttcagaatt gcgtgaaggt tataacacca ttgtgggtga acaaggagcg
     5401 gggctttcag gcgggcaacg ccaacggatt gcgattgctc gagctttggt aaacaacccg
     5461 aaaatcctga tttttgatga ggcaaccagt gccctcgatt acgaatctga gcatattatt
     5521 atgcaaaata tgcaaaaaat atgccaaggc agaaccgtga ttttgattgc acatcgttta
     5581 tcgaccgtca aaaatgcgga tcgaattatt gtgatggaaa agggggaaat tgttgagcaa
     5641 ggcaagcacc acgaattact gcaaaacagt aacggacttt attcctactt acaccaatta
     5701 caacttaatt aagaaggaaa acaatgaaaa tatggcttag tggtatttat gaatttttcc
     5761 tacgctataa aaacatttgg gcagaagtat ggaaaattcg taaagaatta gaccacccaa
     5821 acagaaaaaa agacgaaagt gaatttttac cggcacattt agaactgatt gaaaccccgg
     5881 tttctaaaaa accacgtcta attgcttatt tgattatgct atttttagtt gtggcaattg
     5941 tgcttgccag tgtaagcaaa gttgaaattg tggcgactgc tcccggtaaa ttaactttta
     6001 gtggcagaag taaagaaatt aaaccgattg aaaacgccat tgtacaagaa attttcgtta
     6061 aagatgggca gtttgtggaa aaagggcaat tattagtcag cttaactgca ttgggttctg
     6121 atgcagatat caaaaagacc atggcttcac tttctttagc taaactggag aactatcgct
     6181 accaaacttt gcttactgcc attgaaaaag agtccttgcc ggtgattgat ttatctagaa
     6241 ccgaatttaa agattcatcg gaagaagatc gactacgtat taaacactta attgaggagc
     6301 aatacaccac ttggcaaaaa caaaaaacac agaaaacttt agcgtataag cgtaaagagg
     6361 ctgaaaaaca aacaatattt gcctatgtcc gtaaatatga aggtgcaaca cgtattgaac
     6421 aagaaaaatt aaaagacttt aaggcacttt ataaacagaa gtctttatct aagcacgaac
     6481 ttcttgcgca agaaaataaa ttaattgagg ctcagaatga gctagctgtt tatcgctcaa
     6541 aattaaatga attagaaaat gatctactca atgtaaaaga agaacttgaa ttgatcacgc
     6601 aattctttaa aagcgatgtg ttggaaaaat taaagcaaca tattgaaaat gaacgccaac
     6661 ttcggctcga gttagaaaaa aataatcaac gcagacaggc ctcgatgatc agagcaccgg
     6721 tttccggtac ggttcagcaa ctgaaaattc acactatagg tggtgttgtt acgactgctg
     6781 aaaccttgat gatcattgtg ccggaagacg atgtgttaga ggccaccgct ctggttccaa
     6841 acaaagatat cggctttgtt gcagcagggc aggaggtgat tattaaagtg gaaactttcc
     6901 cttatacacg ctatggttat ctaactggtc gaattaaaca tattagcccg gatgcgattg
     6961 aacaacctaa tgtaggctta gtttttaatg caactatagc tatagatagg aagaatctaa
     7021 catcgcctga tgggcgaaaa attgatttga gttcaggtat gacaataact gctgaaatca
     7081 aaaccggtga acggagtgta atgagttatt tactcagccc attagaagaa tctgtcacag
     7141 aaagtttaag ggaacgctaa tcgaaccaaa acaaagccat aaaagccatt ttgagctttt
     7201 atggctttat tttttagtcc acaagcggtc aaaaaagccc aattttttac acttttataa
     7261 caaattgttc taactaaaaa ttactaattc ttttctttta taagacgaat cctctatttt
     7321 catttcatta acattgaact agaaaagggg atttatgagc ctaagcatta cgaatctttc
     7381 tcttggctac cgcaaaaatc agcaaaggct tatttgaaaa gcacggtgtg caggtggaaa
     7441 aaccggtgat gtttcgcagc tgggctcagt tggtggaagc ctttttaagt ggcaatgtga
     7501 acgtggtgca tctgctttcg cctatgagtt tgtgggcgaa atatggagca aatgctccgg
     7561 tgaaagcggt aatgtggaat cacttggcag gttcggcttt aacggttcgc cctgaaatca
     7621 acagtattgc cgaactctcc ggcaaaacgg tagcaattcc gttttggtat tcaattcata
     7681 atatcgtttt gcaacgatta ttacgagaaa atggcttgaa aatcaccgaa aaagagccga
     7741 aaagcggtga agtgcgatta agtgtaatgg ctccgtcaga tatggtggca gcgttggcaa
     7801 gtgaggcaat ttccggcttt attgtggcag agccgttcaa tgcgttagcg gaagccaaag
     7861 gtgtggggaa ggtgctgcgt tttagtggcg atgtgtggaa agatcacgcc tgctgtttaa
     7921 ccctaatgca cgaatacgat attcaacacc gcccggaatg ggtgcaaaaa gtggtgaatg
     7981 tcttaaccga agcccaagtg tccacgcttg ccaaccgtgc agaaaccgct caattattgg
     8041 ctcggggcag cggctacacg ccgcacgatc aaaaagtgct ggaaaaggta ctggctccaa
     8101 cccaagctca gtgggataaa tatatcgaaa caggggcaat tcaaaaccct cagtggcacc
     8161 aacagcgaat cggtttccaa ccgtatccgt tcgattccta tatggaagag ctggtcaaaa
     8221 tgctcaaaca gacccacatt gccggcaata atgcgttttt acaacagctt gacccggtag
     8281 aggcagcgaa agcgattaac gagccgacct ttgtgcgaaa agcgattgag caaggcggtt
     8341 gggttgaaac ctttaattta caaaccggtt ggacaagaac cgagcaaatt agtgtataag
     8401 cgataggaac gaacgatgaa acagaaatat atttacggct tagccggttt attactgcta
     8461 cttttaggtt ggcagcttgg cacagccttg ttaagccaac ggttgccgat ggcgtcaatg
     8521 ttagctccgg caaaaacctg ggaaagtttt acttggctga tttcctccgg cgaaattttt
     8581 ccgcacttat gggcgagttt gcaacgcatt gctgtcgggc tactcgctgc gttagtgatt
     8641 ggtgtaccgc tgggcttatt gctcggcttc tccaagcacc ttgaaatgct ttgcacgccg
     8701 tcattccaac tactacgtat gatttcgcca ctttcgtgga tgccgattat cgtgatgtta
     8761 cttggtgtag gcgatttgcc gatttatttt ttactggcgt ttgccaccgt ttggtcgttg
     8821 attatttcca ccacctcagg ggtaaaaagc attgatccac aatggttaca actgggtaga
     8881 gtaatgtcgg ctacccgcaa agaactgctg tgcaaaattg ttgtaccggc tattaccgga
     8941 cacgttctca ccggtttacg tgtggcggtt gggattgcgt gggtggtgct ggtaccgtgc
     9001 gaaatgctag gcgtgaatca aggactcggc tattttattt tagacacccg agaccgatta
     9061 gcctattccg aactgatggc ggtaattata attatcggct taatcggctg ggcgttggat
     9121 agcttaatcc gcttggcttt agcgaaaggc ggcagataaa ttgacaagcg gtcgaaaaat
     9181 tgcatttttt tgcaaaaaat cgaccgcttg tttattccca aaaagcatat taccttaatt
     9241 tattttattt ctagggcata aaaaagcgat ttaagatagc gacaaagtaa actaattata
     9301 cgaggtaaac acaatgtcgg ttctttcatc ttcttttatt caatggcttc agcagaacgc
     9361 cgaaaattta gatcaatcta acgaacgagc ggatttactc attccacaaa ttgccgcact
     9421 agggctatat aaaatcggcg tgccggaaac gcttggcggc agcggtggca ctttggttga
     9481 tgcgatcaaa gccgttaccg aactgggtaa tcactcgctg gctgcggcat ttgtttcgtg
     9541 gggacatcgt acctttatcg agcatattat cgcctcaaaa aatcctattc cccgccaaag
     9601 ctatttggcg gatctgctaa atggcaattt aaccgcagga acaggtttat ccaatgcgat
     9661 gaaatttctc tccggcattg aagagttgaa tgtgaatatt cgtgaagaaa acggtaaacg
     9721 ctatttaaat ggccgcctgc cttgggtaac caatttacgt tcataccgct ttgttgctag
     9781 tttcgttgcc ggttttcaag acgaacgcaa accgctagtg attgcaattc cctccaccgc
     9841 caaaggctta acccgcacca aagaattgga atttatcgca ttgcaaggca cgaatactac
     9901 ctctctcgta tttgagaatg tggaattaca agatgaatgg attttggctg atgacgcgcc
     9961 tgagttttta gcagccactc gccctgcgtt cttaggttta caatttggtt tgccctttgg
    10021 cttggttgaa cgtagtttgg cggaaatcga aaaatcgctc cctttccgtg caattctcgc
    10081 ctctgagtgg caagctcaag ttgatgccct caacgatatt aaaactcgtc tgtttaatgg
    10141 cttaaatgaa gaaggttatt ttgtgcgtaa tccgaaagag cttttccaag cgagaattga
    10201 cattgtcaat gtcgtcgccc aaactattct gctagaactg caagcgagtg gcggacgtgg
    10261 ctatctgaaa aatgatactt caggcttcag ccgccgttgg aaagaagcgg catttctgcc
    10321 cgttgttacg ccaagtgcgg tacagctgag aatggtgctg gcgaatagtt aagcggataa
    10381 gagacaaatt gttgcctctt ttattttgca aaaaatagtc taaatataac cgcttgcaat
    10441 aaaaataaaa aaggatgcat tttttatgct tcctcaattt tacggctatc tattatgcaa
    10501 aaggctgcaa ttcagggtta attggaattt cggcaatgtt gttaacgtag ttacataatg
    10561 ttgccaagct cacgcctaaa atcacatcaa tcgcattttg ttggttgtaa cctgcggcaa
    10621 aaaattcgtt taattgggct tcggttaatt tggctttttg taacattact gcaagggtga
    10681 agcgagccag agtgtctaat ttgttatcgt ctatgcgagc cgtggcacgt aaagcattta
    10741 ccacttcttc aggcattttt aatgctttta aggcgatttt agtatgacct gccacgcaga
    10801 aaccacagcc gttgaccact gctgcggtga tttgtaccac ttcacgttct tcagcggtta
    10861 agctgttacg actattaata ccacccacag tgcggtaagt ttccagtgct gttggtgcgt
    10921 tggctaatac gccgatcaga tttgggataa aaccgtttgc ttgttgcact gcttttaggg
    10981 cttcttgtgc cgtttcagga gcggattcaa tagtgtgaat ttgaaattga gacataaatg
    11041 ttccttatca ggttgtttat tggtgtggct catcataaag aaaaggctaa tttagaacaa
    11101 atcaaaatca ctatttaata taactaaaag atcttaaagg gagtatagtt gcctatactc
    11161 cctttgttta tgcacaggtt gcgtttttac tcttctgcgt cttcttttgc ccattctaat
    11221 gagcggcgaa cggctttttt ccaaccttta tagcgtttcg cccgtttttc ttggtcgccg
    11281 tccggtgtga aggtgcgttc aatttccgct ttgtctttca gttcttccaa atctttccag
    11341 aagccgacag caatacccgc aagataagct gcccctaaag cagtgacttc tttcactttc
    11401 ggacgctcaa ctttggtgtc taaaatatcg gcttggaact gcattaagaa gttgtttgcg
    11461 gttgcaccac cgtccacacg taacgcttgt agtttcgctt tggagtcaga ctgcattgct
    11521 tctaacacat ctcgggtttg gtaagcgata gactctaggg tcgcacgcac aatgtggtta
    11581 cggtttgccc cacgggttaa accgaaaatt gagcctcgtg catacggatc ccaatatgga
    11641 gcacctaagc cggtaaaggc aggtacaaca taaacaccgt tagagtcatc ttctttagtg
    11701 gcaaaatatt cgctgtcaaa gctatcgtga acaattttca gctcatcacg cagccattga
    11761 atagacgcac cggcaataaa tactgaacct tccaacgcat attccggctc gccttttgcg
    11821 ttacacgcaa tggtcgttaa taaaccgttt tcagattgaa tcgcagtgtt accggtgtgc
    11881 atcaacataa aacagcctgt accataggta ttcttcgctt gtccttcttt cacgcataaa
    11941 tgaccgtaga gagcggcttg ttggtcacct gcaatacccg cgaccggaat acgcacaccg
    12001 ccttgaccgc cgatattagt ttgcccgtaa acttctgatg aatttttcac ttcaggtaac
    12061 attgagcgtg gaatattgag caattccagc attttatcgt cccattgttt ggtgtgaatg
    12121 ttaaacaaca tagtacgaga agcgttggtg taatcggtaa cgtgtacccg cccttgggtt
    12181 aatttccaca ccagccaagt atcgaccgta ccgaacagca attcgccacg ttctgctttt
    12241 tctcttgccc cttctacgtt atctaaaatc cacttcactt tagtgccgga gaagtacgga
    12301 tcgactacca agcctgtcgt tttacgaata tattcctcgt gtccgtctgc ttttaattta
    12361 tcggtaatat ccgatgtgcg acgacattgc cacacaatcg cattataaac aggtttaccg
    12421 gtctcttttt cccacacaat cgtggtttca cgttggtttg tgatcccgat tgcggcaatg
    12481 ctatccggct taataccggc ttttgccacc acttcattta aggtggaact ttggcttgac
    12541 caaatttcca tcgggttatg ttctacccag cctgcttgcg gataaatctg ggtaaattca
    12601 cgctgagcca cttcaaccac attggcattt ttatctaata aaaccgcacg agagctggtt
    12661 gtaccttggt cgagagcgat gatatattgt ttttccatag attactcctg agtatttgct
    12721 ggaagattgt tatgaatgag gtttttataa ccccaagcac cgaataatgc accgcagatt
    12781 ggggcgacaa tcggaatgat gaaatacgga atttcttttc cgcctgttaa agcaatttca
    12841 ccccaaccgg caagataagc aaagagtttc ggaccaaaat cacgagccgg gttcatcgca
    12901 aagccggtta acgggccggt tgctccacca attaccgcaa tcaataaacc gatgagtaat
    12961 gcggctaagg ctttattcgg caagccgttt ttatcatcgc caatcgctaa aatagtggac
    13021 atcaacacca cagtaatcac aaattcaacg atgaaggcgg taaccaatgt aatattaggg
    13081 tttggatagg tggagaacac accggcaaag ccaacagttt cgccccgagt aatgccttga
    13141 gcggcttcgg cggcagtaaa gagatcgcta tacataaaat aaatcagtgc gacagagcaa
    13201 aacgctccgg caacttgggc gatgacatag ggcaaaattt tatgcttttc aaagccgtag
    13261 aatgcggcaa gtgctatagt gaccgcaggg ttaaggtgag caccggaaat accggcagaa
    13321 atgtagattg ccatggaaac accaagcccc caaacgatgg aaatttccca taaaccgaaa
    13381 ttcgcaccgg ctacctgagc agcagcaaca caaccagcac caaagaaaag aataaaacct
    13441 gtaccgataa attcgccaat acaggcatta cgtaaggatt tgctcataaa gtttctcctt
    13501 aagatgaaaa tgattggaaa gtgatgaaaa aatgtttgaa tatgaacata tttcggagtg
    13561 atattgccaa agcggcattt tttaagcaaa taggaagttt caaatttgtg aaatccatca
    13621 cattattttt ctaaatcact catttttagg tatgatgtga gaaaacgtta gatgaggaaa
    13681 aatgatgtct tcaatactta tgatttcaat cggggcggta cttggagcta tgtgccgctg
    13741 gcaattagcc gtttggctaa acccgatact cagccaattc gcctttggta ctttagcagc
    13801 aaattggctt ggttgtttgt taattggtat tgcaatgggg tttaatttag gcgagagcca
    13861 gcggttgctg ttcatcagcg gttttttagg cagttttacc accttttcca gtttctcttt
    13921 agaactaacg gaaaaactat tagcagaaaa atgggggcag tttgtgacag tgctgagttt
    13981 acatttaatc ggtgggcttt tgcttacttt gataggtgta atgcttgcaa gagccgttac
    14041 aagcggtcgt ttttgctgaa aattttgcaa ataaaaaacc tcccaaacgg gaggttttgc
    14101 gagcgattaa agtaggatat tggaaagtaa taaaccaaat ccgatactga aaatcatact
    14161 aagtaagccc ggtaacataa agctatggtt gaaaatatat ttaccgatac gggttgtgcc
    14221 ggtggtgtcg aagtcaatcg aggcaataat cgggccgtag ttcggaatga agaagtagcc
    14281 gtttaccgcc acaaataccc cgattagcac cggtgcagga atgccgatag caatcgctac
    14341 agggaataag gttgcaaccg ttgccccttg gctgtttacc aatacggaaa gtacgaataa
    14401 tgcgaaagca aatgcccaag gtgcggtttc aactaagcct gaaaccattg atttcacttc
    14461 gtccatatga ccttgcatta aggtatcgcc taaccaagca ataccgaaaa tcgcaatcac
    14521 tgcacgcata ccggcgtgga agacagaacc tttggtgatt tcattaccgt ccggtttgca
    14581 gaagaagata atcaacgcac caatacttaa catcacaatt tcaatcgtgt gagccatccc
    14641 catcggctta ccatcaaaca cagggcgaag agctggaatt gcgcccatta ataccactaa
    14701 taatgcaccg aataggaata agcctactga taatttcgca gtcggtttta acggtaattc
    14761 ggttggattg gtagtaatgt gattttcttt cacatattca gggtcttgca ataagcgttg
    14821 ataatttggg tcgtctttta attctttgcc cattttattt acaaacacac aagctaaacc
    14881 taagcccaat aaggtcgcag gaatagtcac acttaacacg ttacccaatg taacaccttg
    14941 aggttcaagg aaggcaacca cagcaactac tgccgcagca atcgggctgg cgacaatcgc
    15001 aaattgtgag gcgattactg ccattgaaag tggacgctca gggcggatac cgttgtggcg
    15061 gcttacctct gcaattacag gtaatacaga ataagcaacg tgacctgtac cggcaagcac
    15121 ggtgaatgtc caagttacaa gcggtgcgat aaaggtaatg tatttcgggt ttttacgcaa
    15181 aatgcgagtg gcaattttga tcatatagtc caaaccaccg gctgcttgca tggctgctgc
    15241 agccgacaca accgccataa tcataaacat tacatcaatc ggtaaaccgg caggttttag
    15301 cccaaaccca aatgaaagaa tggctaagcc caagccaccg aacaccccta atccgatacc
    15361 gcctacacgg gcgccaacca aaatgcatag taacactatg gcaaattgga taataaacat
    15421 cgcagacata attttttgct cctaaataag taaatgaaac tctgtgtttg aaaaaatctg
    15481 catttttcat caaattaatc ggcacgtact atatcaataa ataaaactaa tctctataat
    15541 ctaaatcaat aaaatttcta attaattaag gaataatatg caattttcaa aaatgcacgg
    15601 cttaggcaat gattttatgg tggttgatgg tgtaacccaa aacgtttacc ttaccgaaga
    15661 gatgatccgt acgctttctg atcgccatcg tggtatcggt ttcgaccaac ttttattggt
    15721 tgaaccgcct tacgaccctg aattagattt tcactatcgc atttttaatg cagacggcag
    15781 tgaagtgtcg caatgtggca atggggcgag atgttttgcc cgttttgtta cgctcaaagg
    15841 cttaaccaac aaaaaagaca ttcacgttag caccgcaaaa ggcaaaatgg tgctaagttt
    15901 gcaagaagac ggcaaagtgc gtgtgaatat gggcgaaccg atttgggaac cttcgcaagt
    15961 gccttttaca gcaaataaat ttgagaaaaa ctatatttta cgcaccgagc tacaaaccgt
    16021 gttatgtggc gtggtttcaa tggggaatcc gcactgcgta ctgcaagttg atgatgtgaa
    16081 aaccgccccg gtaaatgaac tcgggccgtt actagaaagc cacgagcgtt ttcctgaacg
    16141 ggcgaatatc agctttatgc aagtagtgaa tcgcaatcac gttaaattac gggtgtatga
    16201 gcgtggagca ggcgaaaccc aagcctgtgg tagcggtgcg tgcggcacag tggcggtcgg
    16261 cattatgcaa ggcttgttag ataacaaagt gcaggtggat ttacccggtg gaacgctgat
    16321 tattgaatgg caaggagtag ggcatcctct gtatatgacc ggcgatgcca cccacattta
    16381 tgatggattt attaaactgt aagttttccc atagggaggc aagcggttta tttttaccgt
    16441 ttttttgcaa aatagggttg gtttatacaa aagtaccagc gtggacgctg gcactatcag
    16501 actgatgtat aacttttgtt taaattcaac ttatagatgg tgcaagcgtc ctcgcttgta
    16561 ccaaaacagg tttcactcga gtaacttctt caccaactcc aacaccacag gtaattcggt
    16621 aacaccttct ctatccaaaa aatgcccgcc ttgatttaac cgaatataat ccgcgtgcaa
    16681 atgttgggct aacgcatcac tgtattggtg attcacgacc ttatcatcta acgcacttac
    16741 cacatagctt ggaaagctcg atacgctcgg caaaatcgca taatagttgg taaaggcaga
    16801 cagttcgggt aagttttcta ccggttgata gaaccccgac acaaacaccg cccctttaat
    16861 cttggcgtaa tttttggcta aaaaattcaa aatggcaatg caacctaaac tatgcccgac
    16921 aaaaaccgtt tgatcggtca gctccacatt ttgtgcaaga tgatttaacc aaccatctaa
    16981 tgacggcttt gccgaatccg gcatattcaa ccgctcacaa ctgacaccac acttcgctaa
    17041 ttcttgctcc agccaaggaa accaatgctt atcggcattc gccgtataac cgtgtacgat
    17101 ataaacctgt gccatttcac acctcctgat tcaccaaaga ttgtaccata gcttaatcag
    17161 gaaaaaacaa gcggtgattt ttttaaattt ttttgcaact caggtaaaat agcacgcatt
    17221 tacgcaaaga ggtcatcaat gaacgtttgg attttacttg cgatttccat ctgcctcgaa
    17281 atcgctgcca ccaatttact caaattaagc aacggcttca ccaaagctat accgactatt
    17341 ggctcgctcg ccttatacgg tttatccttt tactttctct ctattatttt ccgcacccta
    17401 ccggtcggaa tcgtgtatgc tatttggtcc ggcgtaggga ttgtccttac cgcccttgtt
    17461 gcttattttg ctttcggaca gaaaattgac ttggcaggct taatcggcat cagcctcatt
    17521 attgccggtg tattggtgat taatttgttt tctaaagtgt gatacaagcg gttgatttta
    17581 caaaaaaatc tgcaaaatca accgcttgta atagttggca agaattataa cccctttaac
    17641 cgccggtaaa gctcagtaaa tttttggcgt ttttgttgat attctgcata gcgggcaggg
    17701 gttggttggt aaacctgttc caggggtaac ggctgacaga acgtagcaat atcctgattt
    17761 gggtttagcg caatttgagc gagttttgcc gcccctaatg ccggaccgac atcaccactg
    17821 gtgcggtatt caaaggtttt accgctaata tcggcaagta gttgtcgcca gtacacactt
    17881 tttgctccac cgccaattaa tgcaatattg tcagcaacta caccagtttc gtggagtacc
    17941 tcaatccctt cagccaaagc gaaacttacc ccttcaatca cggcttttgc catcgttatt
    18001 tggttgtcat tatggcttaa tccccaaaat acgcctttcg cataagggtc attatgcggt
    18061 gtacgttcac ccgataaata aggtaagaaa atagcatcag atgccgtttg gcttgcttca
    18121 acctgttgga ataaagtcga tacatctttt agtcccaatg attttactgc ccaatctacc
    18181 gcagaagcgg cacttaatat tactgacatc agatgccaac gatcaggtaa agcgtggcaa
    18241 aaactatgca ctgctttttg cggattagcc tgaaatttat cgctcactac aaaataaacc
    18301 cctgaagtcc ccaatgagag catagcttgc ccggtttggt ataagccaat gccgattgca
    18361 ccggcagcat tatcgccacc gcctgccaca atcggcacag atttcatttt ccattgctcg
    18421 gcaagggttg gacgtaaata acctgtgatt tgattgcctt caaacagttt cggcatattt
    18481 tcaatgtcta atccgcaagc atttagtagc gatttgttcc aattccgttt acccacatca
    18541 agccacattg tgccggaggc atcagacata tcagaggcat attcgccgct catcaacaag
    18601 cggagataat ctttcggtag caagactttg ctgacttttt cggcaatatc gggctggtgt
    18661 ttatcaaccc aacgcaattt cggagcggta aagcccggca tcatcaggtt gcctgtaatt
    18721 tcacggctat ttggcacaag ctgttctaac tcttcgcatt ccataaaact acgtccgtca
    18781 ttccataaaa tcgcagggga aagtacatta tctgcgttat ctagtaaagt cgctccgtgc
    18841 atttgcccgg ttaaaccaat cgcttttact gcttgtaaat cttgagtagc ggaaagttct
    18901 aacatcgctt cattggtggc attccaccaa tcttgcggat tttgttcaga ccacaatggg
    18961 tgtggacgaa aaataggaag tgatttttgt gtgatggcaa taatgtgttg attttcatcg
    19021 agtaacacga ctttcacacc cgatgtgccg agatcgattc caatgtacat aaatattcct
    19081 ttgttattga atacaagcgg tcattttatt cggaacattt gcaattttaa gttttggaga
    19141 tctatcaccc ctctttagca aagaggggga tcagatcgag cttaactttg caaaaaattg
    19201 ataaaaaaca accgcttgta gattaacggt aaatatagct atttaccaaa ttttccaaat
    19261 attcttgctg accggaaaca ggttttggat ctaacccttg ggtttccact atttttgcca
    19321 aatcttcaag gcttgcttta ccgtttagaa tttgtaagcc caaatcttgg ttccagccag
    19381 cgtagcggtt atctactact ttctgtaatg cttgatcttc cagcattttt gccgcacgtt
    19441 ttagcgaaag ggctaacaca tcaattgccc cgatatgtgc gtggaataaa tcatacggat
    19501 cggtactttg gcggcggatt ttcgcatcaa agttgaaacc gcccgttgtg aaaccacctg
    19561 cttttaagat ttcatacatc acaagggtgt tttcttccac gctgtttggg aattggtcgg
    19621 tatcccaacc taattgcgga tcacctcggt tggcatcaat cgagccaaaa atatctaatg
    19681 cggtggcagt cgcaatttcg tgttggaaag tatgccctgc aagagtggcg tggtttgctt
    19741 caatgttgac tttgatctct ttttctagac caaactgttt taagaaaccg taaacggtgg
    19801 caacatcgta gtcatattga tgtttggttg gttcttgtgg ttttggctca attaataaag
    19861 tgccgttaaa accgattttg tgtttatgtt ccaccactaa ctgcataaag cgaccgattt
    19921 gttcacgctc acgttttaag tcagtgttga gtagggtttc ataaccttca cgtccgcccc
    19981 ataatacata attttcgccg cctaagcgtt tggttgcgtt cattgcggta aagacttgtg
    20041 ctgccgccca agcaaagact tccggattcg gattggttga agcacccgac atatagcgag
    20101 gattggtaaa gcagttcgcc gtaccccata ataatttcac gcctgtttca gcttgttttt
    20161 gctctaaaat atccacaata gtgttgaaat tgtgctgata ttctttgaaa ttgttgcctt
    20221 ccggtgcaac atccacatcg tggaagcagt agtaaggaat gcctaatttt tggaaaaatt
    20281 caaaggcaat ttccgctttt tgcttcgcac cttgcagtaa atcccccgtt ttttgccagc
    20341 ttctgtcaag cgaacccacg ccaaacatat cgttacccgt ccagcagaag gtgtgccaat
    20401 agcaaaccgc taaccgcaga tgttctgcca tcgttttgcc taaaaccact tcatccgggt
    20461 tgtaatgttt gaatgcaaac gggttggtgg aattagcccc ttcatattgt acttttgaga
    20521 ttttgtcgaa atagttagac ataataatgc tcctatgaat tggaaaaata atctttcggt
    20581 aacatcatcg gaaaattatg caaaaccttc aattacgata tttcgttttt ggatttcgat
    20641 tattggttaa tgtggcgtag atcacaaaag tgaaaaatca taatgatatt aataaatatg
    20701 ataattgaac ttcatttgaa ataagcccag actgcttacg ggcgaaaaag atgctcacat
    20761 tttcataacc aatgaggtga actatgaaac ttaagtctaa attattagcc attgctacgg
    20821 cgactttaat ggtactcagc aatgcttctg ttgctaaaga tctaaaaatc ggtatgtcaa
    20881 ttgatgattt acgcttagag cgttggcaaa aagaccgaga tatttttgtg aataaagcgg
    20941 aaagcttagg agcaaaagtc ttcgttcaat cggcaaatgg cgatgcaacc gcacaaatct
    21001 cacaaattga gaatatgttg aataaagata ttgatgtatt ggtgattatt ccgtttaacg
    21061 gcgaagtgtt atccaatgtg attgccgagg cgaagaaaga aggtgttaag gtacttgctt
    21121 acgaccgctt aatcaataat gccgatgtgg atttttatgt ttcatttgat aatgaaaaag
    21181 taggtgaatt gcaggctaaa agcattattg ggcaaaagcc ggaggggaat tatttcctaa
    21241 tgggtggttc acctgtagat aataacgcga agctgttccg taaaggtcaa atgaaggtgc
    21301 tacaaccgtt aattgacagt ggaaaaatta aagtggtagg cgatcaatgg gtagattctt
    21361 ggcttgctga aaaagcatta cagattatgg aaaatgcact aactgctaat aaaaataata
    21421 ttgatgcagt tgtagcttca aacgatgcaa ccgcaggcgg tgcaattcaa gcgttaagtg
    21481 cccaagggtt atccagcaag gttgcaattt ccggacaaga tgcagattta gccgcaatta
    21541 aacgtattat tgccggtacg caaacgatga cagtgtataa accaattacc aatttagccg
    21601 acaaagctgc cgaaattgct gttgcattag gtaaagatga gaaagtagaa atgaatgctc
    21661 aattaaataa cggtatgaaa gaagtaccgg catatttatt agagccggtt gttgtaacta
    21721 aagaaaatat tgatgacaca gtaattaaag acggcttcca taccaaagat gcagtatata
    21781 aataaatcac tttaattttt ctagatggcg aggcttcctc gccatttttg aaaataaggg
    21841 gctaactaag tatggcgaaa ttattagaaa tgaagaacat cactaaaaaa ttcggtgagg
    21901 ttgtggcatt aaataatatt tcaatttccc ttgaaaccgg cgaaatactt tctttatgtg
    21961 gtgaaaatgg ttcaggcaaa tcaactctga tgaaagtatt atgtggaatt tatcctgccg
    22021 gtgattatga aggggaaatc tatttttccg gcgaaaaatt gattgccaga aatatcaaag
    22081 atactgaaga aaaaggcatt tcgattattc accaagaatt gaccttagta aaaaatatgt
    22141 cgattttgga gaatatgttt ttagggaatg aaattaccaa atcagggatt acagatgaca
    22201 atgaaatgta tctgcgttgt aaaaccttac ttgaacaagt gcagttggat attgacccaa
    22261 acaccaaagt tggagagcta ggtttagggc aacaacagtt ggttgaaatt gccaaagcat
    22321 tgaataaaca ggtacggttg ttgattttag acgagcctac cgcctcgtta accgaaaaag
    22381 aaaccgagat tctgctgagt ttaattaaag atttacaagc tcacaatatt gcttgtgttt
    22441 atatttcaca caaattgaat gaagtgaaag ccatttccga caaaatctgt gtcattcgag
    22501 acggcgaaca tatcggtact cgcagtgcag agggaatgag tgaagacgat attattacga
    22561 tgatggttgg acgtgagatt acctcgctct acccacacga gccacacaat attggtgaag
    22621 aagtccttcg agttgaaaat ttaaccgctt ggcacccaac caatacacat atcaaacggg
    22681 ttgataatgc taattttgta ctacgccaag gtgaaatttt aggtattgct ggtttggtcg
    22741 gttcagggcg aaccgagatg gcacaatgta tttttggctc ttaccaaggc aaatatcaag
    22801 gtgatatttt tctgcataag caaccgatta aaatcaaaaa ttgtgcacaa gcgattgaaa
    22861 accatattgt gatggtgccg gaagatcgca aaaaacacgg catcattcca attatggggg
    22921 tgggtaaaaa cataactctc tcttcattac cgcaattttg ttttggcaaa aaagtaataa
    22981 atgaaccgct ggaagaaacc attatcaacc aatctattgc aaaactgaaa gtgaaaactt
    23041 cctcgcctga attagcaatt ggacggttaa gtggcggtaa tcagcaaaaa gcaattttgg
    23101 caaaatgcct attgcttaac cctaaaatcc tgattttaga tgagccgacc cgtggtatcg
    23161 acgtgggagc aaaatatgaa atttataagc tcatcaacca attagctaaa gaaggtatgg
    23221 caattatcgt catttcctcc gaattacctg aagttttagg cattagtgat cgtgtattgg
    23281 ttatgcacca aggcaaaatt aaagcagatt tgattaacca agaccttacc caagaaaaag
    23341 taatggaagc ggcactcaag gagtaaccta tgaataagtt aaaatcgatt aatttacaag
    23401 tttatattat gctgattgca attgttgtga ttatgagctt tttctctatt gcaactgacg
    23461 gtgcttattt aagtgccaga aatatctcca acctgttacg ccaaacctcc attaccggta
    23521 ttcttgccat cggtatggta tttgtgatta tttccgcaga aattgattta tcggttggct
    23581 cgctaatggg cttactcggt ggatttgccg ccatcagtaa tgtatggtgg ggctggccgc
    23641 tctcgttaac tattatagtt actttaattt taggcgtcat gctgggtgct tggagcggtt
    23701 ggtgggttgc ttatcaaaaa gtgccgtcct ttattgtaac tcttgccggt atgctggcgt
    23761 ttcgtggtgt attggtcggc ttaaccaacg ggacaaccgt ttcaccgatt agcaatgaaa
    23821 tgacaaccat tggtcaaggc tatttgcctg atatggtggg tatgatttta ggtactatcg
    23881 ggatattttg ctttattgtt tggggtagct accaacgcaa agccagacaa aaactcaatc
    23941 ttactgtgcc ttctatgtcg aaagacacgc tgaaatacgg actagttgca attatcgtat
    24001 taggtgcgat ctacctactc aatgattacc gtggtgtgcc attcccggta ttggtgttgg
    24061 tgattctagc cattgccggc acattcttgg ccagaaaaac cgccttcggg cgtcacattt
    24121 atgcgattgg tggcaacatt gatgccgctc gcctctcggg gatttcagtt gaaaaagtca
    24181 aacttgcgat ttttgcctta aacggcttat tagttgccat tgcaggcttg attttaagct
    24241 cacgtcttgg agccggcgca ccttccgccg ggcaaaatgc cgaattagat gcgattgccg
    24301 cctgcgtgat tggtggagcc agtttagccg gcggtatcgg tagcatttat ggcgtagtta
    24361 ttggagcatt tattattgct ttgctggata acggtatgag tatgcttgat gtgccaacct
    24421 tctggcaata tatcgttaaa ggtggaattc tactgcttgc agtttgggta gataccatca
    24481 gcaagaaaaa agcctaattg gataacaagc ggtcaatctc gactatccaa cgggaaatcc
    24541 ggcataatcg ggctaaaaat gggaactatt gtgcgttttg ccgtcttttt agcatttgct
    24601 acggcaacca gctccaccaa gtgcggtggc atttcgcctt gtttggcaag gttgcaaagg
    24661 taggttatcg ctttaatccg gctcattgag ccttccagtt ccaacacata cgccaccagt
    24721 gccattcgtg catcagcaat ttctcgctgt ttggtggtca ggtttcccac atccacctcc
    24781 gcacgcactg ccggtaactt tttcggcttg gcttccacca ccgccgacat aaagcgggag
    24841 cggatttcgg tttggatttc ttgagggagg tttgagagtg caaattctaa aatttctccc
    24901 ccaactccaa cctttttatg agtagtccat ccttgtttct ttgcctgata caaaactcct
    24961 tgctttgttt taggtaaaca ggctaaactt aactcagcca attcttttgc cgaatagtgt
    25021 gtttttagac tgttttcact cataaaaacc tctttaagct atctgtcggc aaatttttta
    25081 tggtttcgtt gagcataacg ttcaggccaa atttcttcgg gtgatatacc gattgcttcg
    25141 gcaataatac gctcaccctt taaccacgga cgatctacag cgtttttcaa ggtgctggat
    25201 tgactgtagc catgcttcaa tgagagttgt ctaagcgacc aacccgcttt atgtaaagca
    25261 gcgatgatgt cggccctatg ccagtctttc gctgtttttt taatatcttc caatgtactc
    25321 attagataac tcc
//
LOCUS       DANLXS010000013        24957 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.013, whole genome shotgun sequence.
ACCESSION   DANLXS010000013 DANLXS010000000
VERSION     DANLXS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24957
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.013"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..663)
                     /locus_tag="PXD71_000425"
     CDS             complement(<1..663)
                     /locus_tag="PXD71_000425"
                     /inference="COORDINATES: protein motif:HMM:NF012920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6290584.1"
                     /translation="MNELRLLRQKKLKRLIDEVSEGNIADFARKIGKEPSYVARMLYP
                     SDKAGAKPIGEKMALHICHKFVLPLDWFNRDDDGEFIQINNGEEDNIILSVLNIQASA
                     GIGAKNGDVMEVIREIKFNPEQFYNLFRGITPEHIELISVKGDSMEPTFSHGDLLFVD
                     VTIQEYDGDGVYVFTCDDYIFVKRIQKTGRHFTILSDNDRYKPWQLDQEIYIHGKVKV
                     HQS"
     gene            792..1025
                     /locus_tag="PXD71_000426"
     CDS             792..1025
                     /locus_tag="PXD71_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL6290585.1"
                     /translation="MEELKAYLNSLTTKEQESFALRCNTTVGYLRKSISRNSRLGAEI
                     CVAIERETKGLVTRKHLIEDWHLRWPELANDKQ"
     gene            1182..1424
                     /locus_tag="PXD71_000427"
     CDS             1182..1424
                     /locus_tag="PXD71_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6290586.1"
                     /translation="MYGINLNLNEKSSQTQNARILAHLQNGGRITSLSALDDFGCLRL
                     SARIKDLRDRGHDIQSVFITVPNGKKVKQYFMECAK"
     gene            1421..1772
                     /locus_tag="PXD71_000428"
                     /pseudo
     CDS             1421..1772
                     /locus_tag="PXD71_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1910..2194
                     /locus_tag="PXD71_000429"
     CDS             1910..2194
                     /locus_tag="PXD71_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290587.1"
                     /translation="MLLEQFGITGKLAEDFIVHRKAKKAPITETALNGYQREADKAKI
                     PIQKAVEIAIERGWTGFKADWQWQDDQPKHRPKDNMRAEWNNPEAWAEVF"
     gene            2194..2841
                     /locus_tag="PXD71_000430"
     CDS             2194..2841
                     /locus_tag="PXD71_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL6290588.1"
                     /translation="MSLQKTEQNRPLAVLGEQQGKLSGEVESLVDRIFDQLLASCPSI
                     QYWSEKQVATAKKQWILGFAENGIRTVEQVRQGMKALRAKTDDFVPSIGKFIQWCNTI
                     DYHELGLPDLEQLLKLLDRSLRFDFIEIQKLKFKSNAEYWLITDLYARNKKYSWKDET
                     LRNQAEKALVAMAKRIMSGEQIPPPKPLIEEKKKVPIDPRIQRILDEKKQAGAMQ"
     gene            2838..3410
                     /locus_tag="PXD71_000431"
     CDS             2838..3410
                     /locus_tag="PXD71_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL6290589.1"
                     /translation="MTDFDKDTYQTPKYFRNWLNHRYAWFHIDGCSNGKNALYSYWIG
                     KAAEGLDEDTLSGQIAEDFLAENLLDVILERVADWGELLRIFVNPPYSNPLPFVQRAA
                     ELKKAGHIVVMLLPADKSTRWYQVIQENASEVIDIIGGRINFINPLTGEEVKGNNKGS
                     MVAVFDPFVEGIVTRQMPLDKIKELGGYEK"
     gene            3529..3981
                     /locus_tag="PXD71_000432"
     CDS             3529..3981
                     /locus_tag="PXD71_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL6290590.1"
                     /translation="MAKYQMMKLPGGILSPLNDEEAERLKSFQNGEQYEIEIKRQRNP
                     AFHRKVFAFFNFCFDCWSGSHSDWEFQDPTAQFDTFRKHLTVMAGFYVQTWNLKGQVR
                     IEAKSLSYANMEQDEFEQAYKALINAAIKHVFNNTKDENTINQLYSFF"
     gene            4031..4243
                     /locus_tag="PXD71_000433"
     CDS             4031..4243
                     /locus_tag="PXD71_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290591.1"
                     /translation="MKKNVIRYWLSVPFRIIAVLISLSILTLRYIGYLLIYPLAFFVA
                     VGSFIVIGQWKYQECLDDFKSDFNPI"
     gene            4620..4904
                     /locus_tag="PXD71_000434"
     CDS             4620..4904
                     /locus_tag="PXD71_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDL6290592.1"
                     /translation="MANLRKEAKGRECQVRLPGICNHNPETVVLAHYRMAGLNGVGMK
                     PDDIFGAWACSSCHDECDRRTRKLEADYVRQAHAEGVFRTQAILRREGKL"
     gene            4901..5269
                     /locus_tag="PXD71_000435"
     CDS             4901..5269
                     /locus_tag="PXD71_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL6290593.1"
                     /translation="MSDWLEIALPYPPSVNHYWKHTRNGRHYISDKGKAFRAEVVKIC
                     KMFDPFKGAVQTVLQIYYPDKRKRDPDNLEKALWDALKIGGLIEEDDNQILVDKRTIT
                     AGFKKGGMVVLKIKAVGNAN"
     gene            5259..5624
                     /locus_tag="PXD71_000436"
     CDS             5259..5624
                     /locus_tag="PXD71_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290594.1"
                     /translation="MQIDVKGVLNLWGGYVRFTDCKGYPTMQAFMRESPQERTKQRYL
                     ARLDDDTLEKVDRQMRKLQANDKRQFDVLFYKYAVGLENKQVWQKLSLSKTSYHTELL
                     LAERFMEGALAGSEIRLLL"
     gene            complement(5592..5786)
                     /locus_tag="PXD71_000437"
     CDS             complement(5592..5786)
                     /locus_tag="PXD71_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290595.1"
                     /translation="MALSRTEIVNRSNAKRGIKLKAFKLHESTIAQIEQLAQQLNIPQ
                     NQLIKLAVEKLQEQTNFTTG"
     gene            6213..6288
                     /locus_tag="PXD71_000438"
     tRNA            6213..6288
                     /locus_tag="PXD71_000438"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6246..6248,aa:Trp,seq:cca)
     gene            complement(6303..6881)
                     /locus_tag="PXD71_000439"
     CDS             complement(6303..6881)
                     /locus_tag="PXD71_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290596.1"
                     /translation="MIEFDQIDDSFYHFPFRPRPGDTSSDTATPCIGGVDLVSNPEKV
                     ELIPEAAHSPMLKKLLTDLNTPNSKLLTLGCAYWAHKDNRDTSYAYLEFSFREHSVAT
                     NLEFIRSIDEQFEQFLQENKKQLSIEFSVPEQAFDIVSQALFWSIRPFSYFGSEERIL
                     IYFQAGSPRHQDLEIFLDLLHRFLTEYLVVPA"
     gene            7000..7344
                     /locus_tag="PXD71_000440"
     CDS             7000..7344
                     /locus_tag="PXD71_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDL6290597.1"
                     /translation="MPEKNPDIYTQAWNYLVMFLSNHNGWICGVIIAFFTSITKSFLY
                     GKTDTPKRVLAEAILCSVIAGSMRPILIYLNADVSLITPIGAGIGLLGTSAIRQMILK
                     FLNKKAGGENVD"
     gene            7334..7927
                     /locus_tag="PXD71_000441"
     CDS             7334..7927
                     /locus_tag="PXD71_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDL6290598.1"
                     /translation="MWISESKFKQVFPNAVSGIYSIIDKHIEKAGCKTKEQQAMFLAQ
                     CGVECAGYSVFEENLNYSATALLRTFPKYFSQHNVVLYARDKRAIANRVYANRMGNGN
                     EQSGDGWKYRGRGLIQITGKANYIEFAKWEGLPSLVTDPDQITKNLALTVQVAVWYWV
                     SRKLSEFSDILTVTKKINGGTHGLTQRTAYYKKLMAD"
     gene            7928..8257
                     /locus_tag="PXD71_000442"
     CDS             7928..8257
                     /locus_tag="PXD71_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL6290599.1"
                     /translation="MIKYIYLAATVMILGLCGWIWHQSKQIDGLRAENQTQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKIQKIANELRNKVEMAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLH"
     gene            8223..8456
                     /locus_tag="PXD71_000443"
     CDS             8223..8456
                     /locus_tag="PXD71_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290600.1"
                     /translation="MVLLIALSGCTSKTIVSTEYLYPPAAYLVPCERTAFSGKTYGDT
                     VDYLIKVMGERDLCASQIDRIREWQAQTKQGFK"
     gene            8723..9199
                     /locus_tag="PXD71_000444"
     CDS             8723..9199
                     /locus_tag="PXD71_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDL6290601.1"
                     /translation="MDNLFDLKLNINQIAELTGLHRQTVSQRVAGLNPALGSNSKLKL
                     YALRDLILTGLAEKMSADVDSLSPADRRAWFQSENERLKFEKEIGELIPASEVALEMS
                     ALAKTVVQALETLPDILERDCGLQPKDLIRVQQVTDDVRDQMALHIQEVTTDTENE"
     gene            9199..11310
                     /locus_tag="PXD71_000445"
     CDS             9199..11310
                     /locus_tag="PXD71_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDL6290602.1"
                     /translation="MFASAKEIRRDIANAIKAPRRMRVSEAVAEYMRVPLGGGNSVKW
                     DKHTAAYMLEPMDCLNSREYDAVIFVGPARTGKTIGLIDGWITYSIICDPSDFLLVQL
                     TQEKASEHSRKRLDRTFRCSPEIAKRLSPRKNDNNVHDKYFRAGNLLKIGWPSINVLS
                     SSDYKYVALTDYDRWPEDVDGEGDGFSLASKRTTTFMSAGMTLVESSPGKDIVDLKHI
                     PKSTHEAPPTTGILSLYNRGDRRRFYWQCPDCSEYFEPSMANMVGYREDSDFVKASEN
                     ARLQCPHCQTLIAPELKRELNIKGVWLKEGQKIDASGQISGESRKSRIASFWLEGPAA
                     AYQTWAQLTYKLLNAEHEYEMTGSEETLKAVTNTDWGLPYLPRSALEQRRSDELMERR
                     EEVEEKTVPAQCRFIVAAVDVQGGKNRRFVVQMVGYGENGERWLIDRYNISHTLPDSD
                     GVIEKIDPRIPDDWYILISDVLEKRYPLAHNPNHFMPILAMAVDSGGEDGVTDNAYQF
                     WRKCRRDGYAKRVYLVKGDSTKRQKLITKTYPDNTARSDRHSSARGDVPLYLLQTDYL
                     KDRINNALFRDTVGANYIHFPDWLGEWFFNELTYEERGADGKWRKPGKGNNEAFDLFC
                     YAHAIAILRGYERIKWGDENDVPSWAKLPDINPNIIRETPRTAEQAVEIEEKSEKPQP
                     KPTKAKSSWLSGGGRKTGGWL"
     gene            11307..11531
                     /locus_tag="PXD71_000446"
     CDS             11307..11531
                     /locus_tag="PXD71_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290603.1"
                     /translation="MSLYTIDELKQKIRTLDEKIETAQSQVSFNGRSVSYQVAELTKQ
                     RDRYQTMLDEQLAQSGQRTKQHRIKYARFI"
     gene            11531..13030
                     /locus_tag="PXD71_000447"
     CDS             11531..13030
                     /locus_tag="PXD71_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL6290604.1"
                     /translation="MNFLEKTIATLSPKWAANRSRNRYVLNAYEAAQPSRTHKASRES
                     KGANSTVRQSAVSLREQARALDQNHDIVIGILDKMEERVIGSKGIHIEPQPLTLAGEV
                     HEELAGQIRKLWAEWSVKPDVTGLYTRPLLERMLLRTWLRDGEVFVQLVKGKVAGLEH
                     GSQVAFSLEALEPDFVPMQSDEAKNGLVQGVFLNAWRKPTAYQVYLDNPQESNGMYGK
                     VKTVPADNMLHLAFRKRLHQIRGVSMLHGVIVRLADLKEYEESERVAARIAAAMTMYI
                     KKGDAALYDDDSNSDGERLFDIAPGAVIDDLKPGEDIGLINSNRPNVNLETFRNGQLR
                     ATAAGTRSSYSSIARDYNGTYSAQRQELVESFEGYAVLQDAFVAAISRPIYREWLKMA
                     IASQAIKLPPDIDQNSLFNAVYSGPVMPWIDPIKEANAWKERIKGGLATEGQAIRASG
                     NNPAEVKRQRIVEIKENQAEGLKFDTDLTNYGGQDKSFSNQKDEINEDE"
     gene            13042..15003
                     /locus_tag="PXD71_000448"
     CDS             13042..15003
                     /locus_tag="PXD71_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDL6290605.1"
                     /translation="MKTWFNIQAKANDTAEISIFDEIGFWGVTAQQFAKDLKALGNNL
                     KQINLHIHSPGGDVFDGIAIYNLLKNHPANKTVYIDGLAASMASVIAMAGDEIIMPEN
                     AMMMIHKPWGIQGGDADDMRKYADLLDKVESTLIMAYVAKTGKYETDLAEMLKEETWL
                     TGKECVEQGFADKLAEPLVAMACIQSKKLEDYTKMPDKIKNMLFAPQGNAGANPKHEQ
                     PQTPPQAGENPQNVAKPDAMVALAQRNATIKATFAAFGTQFDGLLAECLADVSMTAEQ
                     AKDKLLAKLGENTTPSVPQNHIHAGNGNIVGDSVKQSLMARAGQDTDKTNARDNAYNA
                     MTLRELARASLVDRGVGIAGYTPMQMVGLAFTHSSSDFGQILIDVAHKSLLKGWETAT
                     ENYEQFTTRGTLTDFRPAKRVGLGEFGYLPEVGEGEEYTYGTIGDEGASVALATYGQL
                     FSITRQAIINDDMHLLTKIPEKMGAAAKATITKLVFALITGNATAQDGKKLFDATHKN
                     SLTGAALNVENIDKAIQLMNGFVNSRGEPLSIEPEFMLLPTSLHTKAKQILGSSSVEG
                     ADINSGIINPIRDIVSPIKSPRLQIADPKSWYLLNKEAIEVSYLDGVDSPYIEQQHGF
                     TVDGVSTKVRIDAGVNVIDYRGVVKVTNA"
     gene            15077..15400
                     /locus_tag="PXD71_000449"
     CDS             15077..15400
                     /locus_tag="PXD71_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDL6290606.1"
                     /translation="MAKNYIQDGNTVRLTATKAITSGDVIVAEDLIAIAVSDAAKNDA
                     VVGLTTGVFSVKAKQADDIKQGAVLYWSETEGATLTAGSNKRLGIAWKDSGTSSNQVD
                     VKINV"
     gene            15393..15695
                     /locus_tag="PXD71_000450"
     CDS             15393..15695
                     /locus_tag="PXD71_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6290607.1"
                     /translation="MSSPFEQAIAAADQTIQQTMMSEWLIGGKPYPATFDEAPAIFNG
                     LHSTDDRAIHGTERTLTLFRASGYKPRLDDRVSGNGKKYLVKSYHFVDQLIVLQLE"
     gene            15699..16223
                     /locus_tag="PXD71_000451"
     CDS             15699..16223
                     /locus_tag="PXD71_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6290608.1"
                     /translation="MGSQVTGLKELQASLKKIARQTVPKAAAQAIRTVGRQAMNKAVK
                     SVAAEIGVNQKTIKGRAKMTAKPTPSRLQATIKVNRSHMPMIRVLERKSNRLSPTKGS
                     IRVGKHTVQRGFRQRLANGRTHIMYRQGRKRYGIDVAKVPLSQPLTQAFDRELKNYPE
                     QVQAELAKQIAGKL"
     gene            16220..16615
                     /locus_tag="PXD71_000452"
     CDS             16220..16615
                     /locus_tag="PXD71_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6290609.1"
                     /translation="MKIHTKIRKEVLELLQTKLPEIDNFYNGLPTFIDIEEEQLAVSV
                     YLDDISLQEITVCNNQWDAKLNITIHLKSTESAEDELDEWAEKIREIIDDYPEFEHLE
                     SVALSQYQYEQDQNQRTWHMATLIFDVEY"
     gene            16643..17284
                     /locus_tag="PXD71_000453"
     CDS             16643..17284
                     /locus_tag="PXD71_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290610.1"
                     /translation="MAKTTKVQGTKFRISVGREAQKAITAINLNTATLTIASSGYKKG
                     DAIELTGCGQLDGIYPVLSVTTDQVKLCEEVNWTGKDLPANYTKAKAALVQYSEQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPTTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            17368..17769
                     /locus_tag="PXD71_000454"
     CDS             17368..17769
                     /locus_tag="PXD71_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290611.1"
                     /translation="MTLREKLLANKPKLQPIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGVLLFNPLDINDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            17814..18044
                     /locus_tag="PXD71_000455"
     CDS             17814..18044
                     /locus_tag="PXD71_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL6290612.1"
                     /translation="MPERHFAEYRLFYQEQPFGLWRDDYRTAQLAHLTAMFNRDPKGK
                     APKLAEFMPFFNRTDEVEEIEDDGVADYLAKR"
     gene            18107..18334
                     /locus_tag="PXD71_000456"
     CDS             18107..18334
                     /locus_tag="PXD71_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290613.1"
                     /translation="MFEALDRFMINTIKIAIIVFLVACIFISMPNWWIASILVLFPTA
                     YLLDKYWWRNTEMYRRKMDKLVKEMKNNRQI"
     gene            18388..21939
                     /locus_tag="PXD71_000457"
     CDS             18388..21939
                     /locus_tag="PXD71_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6290614.1"
                     /translation="MSSSNRLTITLDVENSKLLAGFAQSDTVANKFARSFIVNMDQAQ
                     QKARQFADRSTKYLKNIEQAANNLNSSVSKERFWQIANRLQFPTSKVISYANSYTELG
                     NKLKLVTESESQHARAMAAVYDISLKTAQSTQATSSVYQTFAQNAKQLGISQNDVAKL
                     TETVAKSVAISGASSATASNALVQFSQSLLMGKLKAQEFNSLMTQTPSVIQAIAKGLG
                     ITTAELKAMVDNGEMSAEKMIEGLKKAESYVNGQYAKTTTTISGAMQNLSTAAEKWVG
                     EMDSTVGASQKAVAVLNALNAHFDTFAKTALVAGGAFVLFKGYMSVRPFIEARAAIYE
                     NRKAIIAENQARQQTIATRQQELVVLSQQTALKLEQTLQTYNQNKAELEAIAVKQQKI
                     TKEREEIAVAIQAAATRTERLALSAELQALDQKEIALGQQKIAVERQQIATKQALKVA
                     YAENAVAQSAMNAGMVTATTTTRAMNGVLAAARAELNLLKAAALSNPLMTLAMVATTA
                     GTALWGFFESQKAAREEALRYADSLDSVRANIEKMTKAQTDAEMVKLSRSIKEQETAL
                     ASLKQRQAELTREIEQGATVLDDAFGAGVRFEKSAKELAKAQEELKLVTADVEKAQNT
                     LNASLEAQEALQAHVPIANLREEFVKLYPHIDESQIKVDGLNIAIGNFTVKSPEMVIA
                     ANNIANALGGVAGEAMRAAILVANLSSMKLDANGAAIIDPKHLEKIEQIERNNAIASA
                     KGKDKIALQVKDALIKSGMKEGDAGYERLKAAYEQQFTLQNAPKRGGKPKKTKAERSA
                     ESYQNQVAEMTNRLSGLKIDKEDLVKYGGISQYQEVRKLTEDIALNAEKYKNYGTEGV
                     AKLKELAAKIDSEQQLLDIEQFQVDHDKKLEAMQFELDLLGKTRTEKELLQFNNQLDL
                     EAERLKINMTKENIALLDQKIAKIKEEYELHQKKQEETRASVMMGIKEGWNNIETDVS
                     NVAANVANITQNTFDGMADSLTNFVMTGKADFRSFANSILSDLSKMIIKMMLFNAIKQ
                     GATMLFGYQGPTTTVNGNAVYGLGTWATGGYTGNGGKYTPAGIVHKGEYVITKEATSR
                     LGLDYLNYLNYGRRGFASGGGVAVPRVPSSSYQPKSAQSSISVQVINNGEPVDAKVSQ
                     KQQGEQTQITVELMRKIARQEANGMIQNNFRAGGVFA"
     gene            21939..22268
                     /locus_tag="PXD71_000458"
     CDS             21939..22268
                     /locus_tag="PXD71_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6290615.1"
                     /translation="METLQWCVRTDLAVENTPEIMEVKFGDGYTQRSPKGLNNLLRAY
                     TGTIKVKKGEHLAVEAFFAKHRGVSPFYFKDPYTQQNKKVVCSSWPAKMVGLNHWEFS
                     VTFKEVP"
     gene            22268..22984
                     /locus_tag="PXD71_000459"
     CDS             22268..22984
                     /locus_tag="PXD71_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL6290616.1"
                     /translation="MPVSISNQMKLDLAKLEQNAMLDLYEVDLRTLKDKSGNAGSVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEASGFERNSSGPSNRPTLTLSNLFGLVTGIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNAKIPSRIIMADTCTWIYRSAECGYTGKQYFDEKDKATSDPKADKCSHCLNG
                     CELRGNQRNFGGFVSVNKLG"
     gene            22988..23719
                     /locus_tag="PXD71_000460"
     CDS             22988..23719
                     /locus_tag="PXD71_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6290617.1"
                     /translation="MELEQQIIDYALQHEPHEMCGFVVFDGNKNRFIPCENQAEDKAN
                     YFEISDLDYIKAEEKGELVAVVHSHPEPNGKPILSVLDRKMQVQTGLDWWLVHNRQIH
                     KFRNVPHLIGREFKHGTMDCYTLYRDAYMLAGYEMDEFDRQDDWWHTGQNLYLDNIQG
                     QGFERVGTPQIGDVILMQVGADVPNHAAIYIGEQMVIHHSPNRLSKRDLYDGYWLRHT
                     HSIWRHKLADKLDFDGILNDIAVNN"
     gene            23988..24578
                     /locus_tag="PXD71_000461"
     CDS             23988..24578
                     /locus_tag="PXD71_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL6290618.1"
                     /translation="MITVKFYGHLRKFGTDFKLEVKDTAEAIRALCTQIRGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFQAVLGVALIGAA
                     FFLGPVGWGVLGASSAMMMGAMGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETMTVEV"
     gene            24581..>24957
                     /locus_tag="PXD71_000462"
     CDS             24581..>24957
                     /locus_tag="PXD71_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290619.1"
                     /translation="MGLFSRKRKNHTPKEAPETGRSKQIVNIVELLCEGEIEGLVDGF
                     KSIYLDGTKIQNDDDSYNFNNVSGQLNVGTQDQEVLEGYDSSQNEVSVGVEVKKKNGA
                     IVRTVTDERISRLRLTLGVRSLF"
BASE COUNT         7755 a         4972 c         5773 g         6457 t
ORIGIN      
        1 gctttggtgg actttaactt tgccgtgaat ataaatttct tgatctaatt gccaaggttt
       61 atagcggtca ttatcagata aaatagtaaa gtgtctgcct gttttttgaa tcctttttac
      121 aaaaatatag tcatcgcaag taaaaacata cacaccatcg ccatcgtatt cttggatagt
      181 tacatcaaca aaaagcaaat caccgtgtga gaatgttggc tccatagaat cgccttttac
      241 actgatcagc tcgatatgtt caggcgtaat acctctaaat aggttataaa attgttctgg
      301 attgaattta atttctctaa tgacttccat cacatcaccg ttttttgctc ctataccggc
      361 cgatgcttga atattcaata cacttaagat gatattatct tcctcaccat tgttgatttg
      421 aataaattcg ccgtcatcat ctcgattaaa ccaatcaagg ggtagcacaa atttatggca
      481 aatatggagt gccatttttt caccaatagg cttagctcca gctttatcgg acggatacaa
      541 cattcttgct acatagctag gctctttacc tatttttctt gcaaagtcag caatatttcc
      601 ctcactcact tcatcaatta ggcgtttcaa ttttttttgt ctaagaagtc tcaactcatt
      661 catataacct cctctaagga ttacttttta gtaattgtac tcttatttac taaaaggtaa
      721 acaaaccaaa tggtattgat attattttta cctaatggta ttattttggc gtttttaaaa
      781 gaggactcac aatggaagaa ttaaaagcat acctcaattc tttaaccaca aaagagcaag
      841 agagttttgc cttgcgttgt aatacgacag ttgggtattt aagaaaatcg atctcaagaa
      901 attctcgatt aggtgcggaa atttgcgttg cgatagagcg tgaaacaaaa ggtttagtta
      961 ctagaaagca tttaattgaa gattggcact tacgctggcc ggaattggca aatgacaagc
     1021 aataaaggga aagttttttc taaaaaactc aaatcattct accacagtaa aaattcaatt
     1081 tttattagag tggcaatttt tatgctgctt gcgtggtggg tttttaacaa ttaaaaaaac
     1141 caccgctgta acggtggcta attactaagg aggtttccta tatgtatggg ataaatctta
     1201 acctaaacga aaaatcaagt caaacccaaa atgcaagaat tttggcacat cttcaaaatg
     1261 gcggtcgtat tacttcgctt tctgcgttag acgatttcgg ctgcttgcgt ttatccgctc
     1321 gcattaaaga tttgcgtgac cgtggtcacg atattcaaag cgtatttatt acagtgccga
     1381 atggcaagaa agtgaagcag tattttatgg agtgtgcgaa atgagattta gcacttacat
     1441 caacaatgca aaatgccttg aatggaactt aaatgctaat caaggagcat tatttgattt
     1501 actgaatcaa gctcctgctt gggcgaaaga aatcattatt gatggcgtgg tttattactg
     1561 ggtttcccgt aatgcggtga tcgaagaact acctttgttt tactcaaagc ctgataccgt
     1621 ctatcgccat tttgtcgagt ttcacaaaaa agggttgatc gtgtatcaaa aacacggaga
     1681 taaagacctt attcgattaa ctgaaaaagg gaagacttgg aatgagttta actcggattt
     1741 aaatccgaca ctcggaaata aatccgagtt agctcggaaa cagatccgaa aaagctcgga
     1801 tttaaatccg acaaataata atactaatta taaaaatact aatgatcata ataataccct
     1861 tacgggtatt aacgcacaca cacgtgagac taaaaaatct gctgtgttgg tgttgcttga
     1921 acaattcggc attacaggca aattggcgga ggattttatc gtacaccgca aagccaaaaa
     1981 agccccgatt accgaaactg cactgaatgg ctatcagcga gaggcagaca aagccaaaat
     2041 cccgattcaa aaggcggtag aaattgcgat tgaacgtggc tggacaggat ttaaagccga
     2101 ttggcagtgg caagacgatc aacctaaaca tcgcccaaaa gacaatatgc gagctgaatg
     2161 gaataaccct gaagcgtggg cggaggtgtt ttgatgagcc tgcaaaaaac agagcaaaat
     2221 cgaccgcttg cggtgttagg tgagcagcaa ggcaaattat ccggtgaggt tgaaagcctt
     2281 gtggacagaa tatttgacca gttgcttgcg agttgcccaa gtattcaata ctggtcggaa
     2341 aagcaagttg ccacagccaa aaaacaatgg attttaggct ttgcggagaa cggaatccgc
     2401 acggttgagc aagttcgaca gggtatgaaa gcgttaagag caaaaactga tgatttcgtc
     2461 ccgagtatcg gcaaatttat ccagtggtgc aatacgattg actatcacga attaggatta
     2521 cctgatttag agcagttact caagcttttg gatcgctctt taagatttga ttttattgag
     2581 attcagaaac ttaaatttaa atcaaacgct gaatattggt tgataactga cctatacgca
     2641 cgtaacaaga aatatagttg gaaagatgaa accttacgta atcaagcaga aaaagcctta
     2701 gtggcaatgg caaaacgaat tatgagcgga gagcaaattc caccacctaa accgctaatc
     2761 gaagagaaaa agaaagtgcc aatagatcca cgcattcagc gaattttgga cgagaagaaa
     2821 caagcaggag caatgcaatg acagattttg ataaagacac ttaccaaacg ccaaagtatt
     2881 tccgtaattg gctaaatcat cgctatgcgt ggtttcacat tgacggttgt tctaacggca
     2941 aaaacgcatt gtatagctac tggattggca aagcggcaga gggcttagac gaagacacac
     3001 taagtggtca gattgccgag gatttcttgg cagaaaactt gctcgatgtg atcttggaac
     3061 gtgtggcaga ttggggcgaa ttactccgta tcttcgttaa tccgccatat agcaatccac
     3121 tcccttttgt tcagcgtgct gcggagctga aaaaagccgg tcatattgtg gtaatgctgt
     3181 tgccggcaga caaatctact cgttggtatc aggttatcca agaaaacgct agtgaggtga
     3241 tcgacattat cggtgggcgt attaatttta ttaatccgct cacaggcgag gaagtgaaag
     3301 gcaacaacaa aggctcgatg gtagcggtgt tcgacccgtt tgtggaggga atagttaccc
     3361 gtcaaatgcc gctggataag attaaggagc tgggcggcta tgaaaaataa cgaagggtgg
     3421 gaagtgtgat gagtgattct actattggat ttttagggat tctaattttc ttcgggtggt
     3481 tagcatattt ggtatttaga gcttttggat tttcattgag ggatagagat ggctaaatac
     3541 caaatgatga aattgccggg tgggatttta agcccgttaa acgatgaaga agccgagcgg
     3601 ttgaaatcgt ttcagaatgg cgagcagtac gaaatcgaga ttaaacgcca gcgtaatccc
     3661 gcttttcatc gcaaggtgtt tgcgttcttt aatttctgtt ttgattgctg gagtggtagc
     3721 cattccgatt gggagttcca agatccaacc gctcaatttg atactttccg caagcattta
     3781 actgtaatgg cagggtttta tgtgcagacg tggaatttaa aagggcaagt gcggattgag
     3841 gcgaaaagcc taagctacgc caatatggaa caagacgagt tcgagcaggc ttataaagcg
     3901 ttgattaatg cagctatcaa gcacgttttt aacaatacca aagatgagaa tacgataaat
     3961 cagttgtatt cgttttttta attaaggcga aatcgccata tttaattagg tttaattgca
     4021 ggagattaaa atgaaaaaga atgttattag atattggctt agcgtgccat tcagaattat
     4081 tgcagtgctt atttctttgt caattcttac tcttagatat atcgggtact tattgattta
     4141 tccattagca ttttttgtgg cagtcggtag ttttattgtt atcggtcaat ggaaatatca
     4201 agaatgcctt gatgacttta agagcgattt taatccgatt tagtgtgtgt ttattaattc
     4261 cctcgaattc gggggtagga gaaatcaaaa tgacaaaaat tctttgaaac tatcgcactc
     4321 gtgatgttat ttggcattat tccaagctgt ttattggcga ttaatactac tttaatgaat
     4381 attaaaaacg caccgtggtt tatggtgatt attcagttag ggttttggat tttaatatcg
     4441 tttgttatcg gttgcggatt attcctttta tgtggaatgt tatttaattt aaggagatta
     4501 ataagtgagc cagtgcaatg aactgactgt aattttaatc actatcgcta ttcttagtgt
     4561 atgtgccggt tcactctttt ttattgagtg gtttgataaa agagaccaag gaggttggaa
     4621 tggctaatct acgcaaagag gcaaaaggca gagagtgcca agtccgttta ccgggtatct
     4681 gtaatcataa tccggaaacg gtggtattgg ctcactatcg aatggcagga ttaaacggtg
     4741 tgggaatgaa acccgatgat attttcgggg cgtgggcttg cagtagctgc cacgatgagt
     4801 gcgaccgtag aactcgcaaa ttagaagcgg attatgtgag acaggctcat gcagagggcg
     4861 tatttagaac gcaggctatt ttacggagag aagggaaatt atgagtgatt ggcttgaaat
     4921 tgcgttgcct tatccgccga gtgtgaacca ttactggaag catacccgaa acggtaggca
     4981 ttatatttca gataagggga aagcctttag ggcggaagtg gtgaaaatct gcaaaatgtt
     5041 tgatccgttt aaaggagcgg tacaaacggt attgcagatt tactatcctg ataaacgcaa
     5101 gcgtgatcca gataatttag aaaaagcctt gtgggacgca ttaaaaatcg gtggcttaat
     5161 tgaagaagac gataatcaaa ttttagtcga taagcgaacc attacggcag gttttaagaa
     5221 agggggaatg gtggtgttaa agattaaggc ggtgggtaat gcaaattgat gtaaagggtg
     5281 tattaaattt atggggtgga tatgtgcgtt ttaccgactg caagggctat ccgacaatgc
     5341 aggcatttat gcgtgagtct ccacaagaga gaacaaaaca gcgttattta gcccgcttag
     5401 atgatgatac gcttgagaaa gtcgatcggc aaatgcggaa gctacaagcg aacgacaagc
     5461 gacaattcga tgtactattt tataaatatg cagtagggtt agagaataag caggtttggc
     5521 agaagttatc gctcagcaag acttcatatc atacagaatt acttcttgct gaacgattta
     5581 tggagggggc attagccggt agtgaaattc gtttgctctt gtagcttctc aacggctaat
     5641 ttaattagtt gattttgggg aatatttaac tgttgggcaa gttgctcgat ttgtgcgatt
     5701 gtgctttcgt gcagtttaaa ggctttaagt ttaattcccc gttttgcgtt gctacggttt
     5761 acaatttcgg ttcttgacag tgccatcata aatccttaat ataggttttg aagagtaggg
     5821 agatttctcc ccctacttag tttcattcaa ttagtatgct ggcaggctaa tcactaagag
     5881 aatgataact aggataatgc ggtaaagcat ttgttatccc tccaaaagtc ggtgggatta
     5941 aaggtcgcca ccggctcgtt tccagcacta ttactgaaaa caagtttatt atatgttgga
     6001 taaccaatat attcaagcaa aatatcgtta aaaagcccat ggttttgtaa attatgggct
     6061 tttttgcatt taaatgatta aaaagtgttg caaagttcgg acttaatcta tattatttat
     6121 ggtaagttgc ggttcattgc ataggtggtg aacttaaaag aattttacag ccctgagcag
     6181 aaatgcttgg ggcttttttg tttacggatt ttaagggtgt agctcaatag gtagagcaac
     6241 ggtctccaaa atcgttggtt gttggtttga gtccaaccac cctttccaaa tgcgagtaaa
     6301 tgttatgctg gaacaactaa atactcagtc aaaaagcggt gtagtaaatc caaaaagatt
     6361 tctaaatcct gatgtcgagg actaccagcc tgaaagtata tcaagatgcg ctcttcgctt
     6421 ccgaagtaag aaaatggacg aatactccag aaaagagctt gtgaaactat atcaaatgct
     6481 tgctcaggca cggaaaattc aatagataac tgctttttat tttcttgcaa gaattgctcg
     6541 aactgttcat caatacttct gataaattct aggttagttg caactgaatg ttcccggaat
     6601 gaaaactcta agtaagcgta tgatgtatct ctattgtctt tatgagccca ataagcacag
     6661 cctagtgtta gaagtttgct gtttggagtg ttcaagtctg tgagtaactt ttttaacatt
     6721 ggggagtgtg cggcttctgg tattagttct accttttcag gattggaaac aagatctact
     6781 ccaccaatac aaggtgtggc agtgtcgctt gaagtgtcgc ccggacgtgg tctaaatggg
     6841 aagtgataga aactatcgtc aatttgatca aattcaatca tttttaacct ctgctagttt
     6901 atttgttggg gaacagtatt ctagcagaat ttttaaccaa ggctcagttt tacgactggg
     6961 cttttttatt gcctcgaaac ggggtggagt atgaatacaa tgcctgaaaa gaaccctgat
     7021 atatatacgc aggcttggaa ttatcttgta atgtttttaa gcaatcataa tggctggatt
     7081 tgtggcgtta tcattgcttt ttttacatct attaccaagt catttctgta tggtaaaaca
     7141 gatacaccta aacgagtgct ggcagaggct atcttgtgta gcgttattgc tggctcaatg
     7201 cgtcctattt taatttatct caatgctgat gtatcactga ttacccctat tggtgcggga
     7261 attggattat tgggtacaag tgcaattcgc cagatgattt taaaattctt gaacaaaaaa
     7321 gcaggtggtg aaaatgtgga ttagtgaaag taagtttaaa caagtattcc ctaatgcagt
     7381 tagtggtatt tactcaatta ttgacaaaca cattgaaaaa gcaggctgca aaacaaaaga
     7441 gcaacaagcg atgttcttgg ctcagtgtgg tgtagaatgt gccggatata gtgtatttga
     7501 agaaaacctt aactattctg caactgctct attgcgaaca ttcccgaaat actttagcca
     7561 acacaatgtc gtactttatg ctcgagataa gcgtgctatt gctaatcggg tgtatgcaaa
     7621 tcgaatgggt aacggcaatg aacaaagtgg cgatggttgg aaatatcgtg ggcgtggctt
     7681 aattcaaatt accggtaagg caaattatat cgagtttgct aaatgggaag gcttaccaag
     7741 tctagtgact gaccccgacc aaatcactaa aaatctagca ttgacagtac aggtggctgt
     7801 ttggtactgg gtaagtcgca agttatccga atttagcgac attcttactg ttactaaaaa
     7861 gattaatggc ggaacgcacg gtctaactca gcgtacagct tattataaaa agctaatggc
     7921 ggactaaatg attaaataca tttatctagc tgccacagta atgattttgg gtttgtgtgg
     7981 ctggatttgg caccagtcca agcagattga tggcttaaga gccgaaaacc aaacacaagc
     8041 ccaaaccatt gagcagcagc aaaaagccaa tcaacggcta accgatagct tagagcaaga
     8101 gcgacaagcg gtcgaaaaaa tccaaaaaat tgcaaatgag ctgcggaata aagtggagat
     8161 ggccaagaat gaaattacat caatactggc acaagacagt tgtgctaagg ctgatttgcc
     8221 taatggtgtt gctgatagca ttaagcggtt gcactagcaa aacaatcgtc agcactgagt
     8281 atttatatcc gcctgcggct taccttgtgc cgtgcgagcg gacagcattt agcggtaaaa
     8341 cctacggcga tacggtggat tatctcatta aagtaatggg agagcgtgat ttgtgtgcga
     8401 gtcaaattga ccgtattaga gagtggcaag cacagactaa gcaagggttt aaataacttg
     8461 gcggattaaa ccgctaatga gagtgggagc ttaattgctc ccttttcatt tgcgttacat
     8521 tacttagtta tgcttttgat tgtatcaaga tcataactaa gcaatgtgtt gcatagtttg
     8581 taaaaggtac tcccgagggg ataccccttt ccacggggtt gcggcagcgt ggttttcggc
     8641 aattttttga gtttctaggc atcatcatct tcctatgatg ttgctgtttt ttaggcggtt
     8701 ttgattttta ggatttgagc aaatggacaa cttatttgat ctaaaactta acatcaatca
     8761 gatcgccgaa ctcacaggat tgcaccgcca gacggtatct cagcgagtcg ctgggttaaa
     8821 tccagcactc ggtagtaata gcaaattaaa actctatgct ctgcgtgatt taatcctcac
     8881 cgggttggcg gaaaagatgt cggcagatgt cgatagccta tcacctgctg atcgtcgtgc
     8941 gtggttccag tcggaaaacg aaagactaaa atttgaaaaa gaaattggcg agcttatccc
     9001 agcaagcgaa gtggctcttg aaatgtcagc tttagcaaaa acggtagtac aagccttaga
     9061 aaccttgcct gatattttag agcgagactg cggattgcag cctaaagatt tgatacgagt
     9121 gcagcaagta actgacgatg tacgagacca aatggcatta catatccaag aagttaccac
     9181 tgacacggag aatgaataat gtttgctagt gctaaagaaa ttcgacgtga tatagcaaat
     9241 gccataaaag cacctcggcg aatgagagtg tcagaagcag tagcggaata tatgcgtgta
     9301 ccacttggcg gaggcaactc ggtcaaatgg gataagcaca ccgccgccta tatgttagaa
     9361 ccgatggact gcctcaatag ccgagaatac gatgcggtga tttttgtcgg cccggcacga
     9421 acgggtaaaa ccatcggctt gattgacggc tggataacct actcaattat ctgcgatccg
     9481 tctgatttcc tgttggtgca acttacccaa gagaaagcta gcgaacatag ccgaaaacgg
     9541 cttgaccgca cattccgctg ttcgcccgaa attgccaagc ggctaagccc acgcaaaaac
     9601 gataacaatg tccacgacaa gtatttcaga gcgggcaact tgctcaaaat cggctggccg
     9661 tctatcaatg tgctttcttc atcggactac aaatatgttg ctctgactga ctacgaccgc
     9721 tggccggaag atgtggacgg agagggcgac ggtttctcgc ttgcctcaaa gcggacgacc
     9781 acctttatgt cagcaggaat gactttggtg gagagttcgc ccggtaaaga tattgttgat
     9841 ctcaaacata tcccgaaaag cacccacgag gctccaccga ctaccggtat tttaagcctc
     9901 tataatcgag gtgatcgccg tcggttttac tggcaatgcc ccgactgctc cgaatacttc
     9961 gagccgtcaa tggcaaatat ggtcggctac cgtgaagaca gtgattttgt caaagccagc
    10021 gaaaacgcac ggttacaatg ccctcactgc caaaccctga ttgcccccga actcaaacga
    10081 gagctaaata tcaagggcgt atggctcaaa gaggggcaaa aaattgatgc aagcggtcaa
    10141 attagtggcg aaagtcgcaa atcccgtatt gcctcctttt ggcttgaagg gccagcggca
    10201 gcctaccaaa cgtgggcgca gctcacctac aaactgctca atgccgaaca cgaatatgaa
    10261 atgactggca gtgaggaaac cctcaaagcg gtaacaaata ccgactgggg gttaccttac
    10321 ttaccacgat ctgcattaga acaacgccgt tctgatgagt tgatggaacg ccgagaagaa
    10381 gtagaagaga aaactgtacc agctcaatgt cgctttattg tggcggcggt ggacgtacag
    10441 ggcggtaaaa accgccgatt tgtggtgcaa atggtcggtt acggtgaaaa tggcgaacgc
    10501 tggttgattg accgctataa catctcacat actttgcccg acagtgatgg cgtgattgaa
    10561 aaaattgacc ctcgtattcc cgatgattgg tacatactga tttcggacgt actagaaaaa
    10621 cgctacccac ttgcccacaa tccaaaccac tttatgccga ttttggcgat ggcggtggac
    10681 agtggcggtg aagatggggt aaccgataac gcctaccaat tttggcgcaa atgccgccga
    10741 gatggctatg cgaaacgagt ctatttagtc aaaggcgaca gcaccaaacg gcaaaagctg
    10801 attaccaaaa cttaccccga caataccgca cgaagtgacc gccactcctc cgcacggggc
    10861 gatgtgccgt tgtacttgct ccaaaccgac tacctcaaag atcggattaa caacgccctg
    10921 ttccgtgata cggtgggggc gaactacatc cacttccccg actggctagg tgaatggttt
    10981 tttaacgaat tgacctacga agaacgagga gcggacggca aatggcggaa accgggcaaa
    11041 ggcaataacg aagccttcga cctgttctgc tatgcccacg ccattgcaat tttacgaggc
    11101 tacgaacgaa tcaaatgggg cgatgaaaac gatgtgccaa gctgggcaaa actgcccgac
    11161 atcaacccta atatcattcg ggaaacgcca agaacggctg aacaagcggt cgaaattgaa
    11221 gaaaaatccg aaaaaccaca acctaaaccc accaaagcaa aaagcagttg gttaagtggt
    11281 ggcggtagaa aaacaggagg ctggctatga gcctttacac catagacgag cttaaacaaa
    11341 aaatccgcac gcttgatgaa aaaatcgaaa ccgcccaaag ccaagtgagc tttaatggtc
    11401 ggtcggtttc atatcaagtg gcggaactaa ccaaacagcg tgatcgctat caaacaatgc
    11461 tggatgaaca acttgcccaa agcgggcaac gcaccaaaca gcatcgaatt aaatatgcga
    11521 ggtttatttg atgaacttcc tcgaaaaaac cattgccacc ctttccccga aatgggcggc
    11581 aaatcgctct cgaaatcgct atgtgctaaa tgcctatgag gcggcacagc ctagccgaac
    11641 ccataaggca agccgagaga gcaaaggggc aaactctact gttcgccaaa gtgcggtcag
    11701 cctacgggaa caagcccgag ccttagacca aaatcacgat attgtgatcg gcattctcga
    11761 caaaatggaa gagcgggtga ttggctcaaa aggcattcat attgaaccgc agccgctgac
    11821 gttggcaggt gaagtccacg aagaattagc tgggcaaatc cgaaagctgt gggcggaatg
    11881 gtcggtgaaa cctgatgtga cgggattata cactcgccca ttgctagaac gaatgctact
    11941 tcggacttgg ctacgagacg gcgaagtctt tgttcagttg gtgaaaggca aggtggcagg
    12001 gttagaacac ggctcacagg ttgctttttc ccttgaagcc ttagagccgg attttgtgcc
    12061 aatgcaatcc gatgaggcaa aaaacggctt ggtgcaaggc gtgtttctca atgcgtggcg
    12121 gaagccgact gcttaccaag tctatttaga caatccgcaa gaaagcaacg gaatgtacgg
    12181 caaagtgaaa accgtgccgg cagacaatat gttgcacctc gcctttcgca agcggttaca
    12241 tcaaattcgt ggtgtaagta tgttgcacgg tgtgattgtg cgattggcgg atctgaaaga
    12301 atacgaagag agcgaacgag ttgccgctcg cattgccgcc gcaatgacga tgtacattaa
    12361 aaaaggcgat gcggcactgt atgatgacga cagtaacagc gatggcgaac gcctgtttga
    12421 tattgccccc ggtgcggtga ttgatgattt aaaaccgggt gaagacatcg gcttaatcaa
    12481 ttccaaccgc ccgaatgtga atttggaaac tttccgcaat ggacaactgc gagccactgc
    12541 cgccggtact cgttcgagct attccagcat cgcccgagac tacaacggca cttactctgc
    12601 acagcgacaa gagctggttg aaagttttga aggttatgcg gtgctacaag atgcgtttgt
    12661 ggcggcaatc agccgcccga tttatcggga atggctcaaa atggcaatcg cctcacaagc
    12721 gatcaaactg ccgcctgata ttgaccaaaa ctctctcttt aatgcggttt attcaggtcc
    12781 tgtaatgccg tggattgacc caatcaaaga ggcgaatgcg tggaaagaac gaattaaagg
    12841 cggtttagcc accgaagggc aagcaatccg agccagtggc aataacccag ccgaagtcaa
    12901 acggcaacga attgtcgaga tcaaagaaaa tcaagccgaa gggctaaaat ttgataccga
    12961 tctcaccaat tacggtgggc aggataaatc tttctccaat caaaaagatg aaattaacga
    13021 agatgaataa caggagggtg cgtgaaaacg tggttcaaca ttcaagccaa agcgaatgac
    13081 accgccgaaa tctcaatttt tgacgaaatc ggcttttggg gtgtcaccgc ccaacaattt
    13141 gctaaagact taaaagccct tggcaacaat ttaaaacaaa tcaatctgca tatccactcg
    13201 cccggtggtg atgtgtttga cggcattgcc atttacaacc tgctaaaaaa tcaccctgct
    13261 aacaagaccg tctatattga cggtcttgcc gcatcaatgg ctagcgtaat tgcaatggca
    13321 ggtgatgaaa tcattatgcc tgaaaacgct atgatgatga tccacaaacc gtggggcatt
    13381 caaggcggtg atgcggatga tatgcgtaaa tatgcggatt tactggataa ggttgaaagt
    13441 acactgatta tggcgtatgt cgccaaaaca ggtaaatacg aaaccgattt ggcagaaatg
    13501 ctgaaagaag aaacttggct cacaggcaaa gagtgcgtgg agcaaggttt cgccgataag
    13561 ttagccgaac cccttgtggc gatggcttgt atccaatcca aaaaattaga ggactacaca
    13621 aaaatgcctg acaaaatcaa aaatatgcta ttcgcaccac aaggcaacgc aggtgcaaat
    13681 ccaaaacatg aacaacctca aacaccacca caagcgggtg aaaatccgca aaatgtggca
    13741 aaacctgatg caatggtggc attagcccaa cgcaatgcaa cgattaaagc gacctttgcc
    13801 gctttcggca cacaatttga cggcttgctg gcagaatgtt tagctgatgt gtcaatgacc
    13861 gctgagcaag ccaaagacaa gctccttgcc aaactgggcg aaaacaccac cccaagcgta
    13921 ccgcaaaacc acattcacgc aggtaacggt aacatcgttg gcgacagcgt gaagcagtcg
    13981 ttaatggctc gtgctggtca agataccgac aaaaccaacg caagagataa tgcctacaat
    14041 gcaatgacct tgcgtgagtt ggctcgtgcc tcattggttg atcgtggcgt gggtattgcg
    14101 ggctacaccc caatgcagat ggtcggtctt gcctttactc attcgagctc cgacttcggt
    14161 caaatcttga ttgatgtggc tcataaatcc ctactcaaag gctgggaaac cgctaccgaa
    14221 aactacgagc agtttaccac ccgtggcact cttactgact tccgaccggc aaaacgagtc
    14281 ggtttaggtg agtttggcta cctgccggaa gtgggcgaag gtgaagaata cacctacggc
    14341 acaatcggtg atgaaggtgc atctgttgca ttggcgactt acggacaact gtttagcatt
    14401 acccgtcagg cgattatcaa tgacgatatg catcttttaa ctaaaatccc tgagaaaatg
    14461 ggtgcagcag ccaaagcgac tattaccaaa ctggtgtttg ccttgattac cggcaatgcg
    14521 acagcccaag acggtaaaaa gctgtttgat gctacacata aaaacagctt aacgggtgcg
    14581 gcattgaatg tggaaaacat tgacaaggca atccagttaa tgaatggctt tgtaaacagc
    14641 cgtggtgaac cgctctctat tgagccagaa tttatgctgt taccaacctc attgcatacc
    14701 aaagcaaaac agattcttgg ttcgtcaagc gtggaaggtg cagacatcaa cagcggtatc
    14761 attaacccga tccgtgatat tgtcagcccg attaaatcgc ctcgcttaca aattgcagat
    14821 ccgaaatcat ggtacttgct caataaagag gcgattgaag tctcttatct ggacggggtg
    14881 gatagccctt acatcgagca acagcacggc tttacggtgg acggtgtttc gaccaaagtc
    14941 cgtatcgatg ctggtgtgaa tgtgattgac taccgtggtg tggtgaaagt gaccaacgct
    15001 taatttaaac aatttaaaac ctgaccgcac ttttaacagt gcggtttttt attatccgaa
    15061 aaaaggaaaa agagatatgg ctaaaaacta tattcaagac ggcaataccg tgcgtttaac
    15121 tgcaaccaaa gcgattactt ccggcgatgt cattgtggcg gaggatttaa ttgcgattgc
    15181 ggtttctgat gcggcaaaaa atgatgcagt agttggctta actaccggtg tttttagcgt
    15241 aaaagctaaa caagcagatg acatcaaaca aggtgcagtg ttgtactggt ctgaaacaga
    15301 gggcgcaact ctcaccgcag gctcaaataa acgacttggc attgcgtgga aagactctgg
    15361 cacatcatct aatcaagttg atgtgaaaat caatgtctag cccgtttgag caggcaatag
    15421 ctgccgccga tcaaactatt cagcaaacta tgatgagtga gtggttgatt ggcggcaagc
    15481 cttaccctgc aacttttgat gaagcccccg ctatttttaa cggtttacac tcaaccgatg
    15541 acagagctat tcacggcacg gaacgcacac tgaccctatt tcgggcgagt ggctacaaac
    15601 cgagattgga cgaccgagtg agcggaaatg gcaagaaata ccttgttaaa tcctatcact
    15661 ttgtcgatca gttaatcgta ttgcaattgg agtaaacaat gggttctcaa gtgaccggtt
    15721 taaaagaact gcaagcctcc ctgaaaaaaa tagctaggca aaccgtacct aaagcagccg
    15781 ctcaagcaat ccgtactgtt gggcggcaag caatgaataa ggcggttaaa tcggtggcgg
    15841 cagagattgg cgtaaatcaa aagacaatca aaggtagagc aaaaatgacg gcaaagccca
    15901 cgccgtcaag attgcaagcc acgattaagg ttaatcgatc acatatgccg atgattcggg
    15961 tactagagcg caagtccaac cgcctgtcac ccaccaaagg cagtattaga gttgggaaac
    16021 acactgtaca acgtggtttt cgccaacgac tcgccaatgg gcgaacgcac attatgtacc
    16081 ggcaaggtcg taagcgttac ggcattgatg tagcaaaagt gccgttatcc caaccactta
    16141 cacaagcatt tgatcgagaa ttgaaaaact atcccgaaca agtgcaagcg gaattagcta
    16201 aacaaattgc ggggaaatta tgaaaatcca cacaaaaatc cgaaaagaag tccttgaatt
    16261 attacagact aaattgccgg aaattgataa tttttataac gggctgccaa cttttattga
    16321 tattgaggag gaacaacttg cagtatcggt ttatctcgat gatatcagtc tgcaagaaat
    16381 aactgtttgt aataatcaat gggacgccaa gctcaatatt acaatccatc tgaaatctac
    16441 cgagagtgca gaagatgaat tagacgaatg ggcggaaaaa attcgtgaaa ttatcgatga
    16501 ttatcccgaa tttgagcatt tagagtcagt cgcgctctct cagtatcaat atgagcaaga
    16561 ccagaatcag cgtacgtggc acatggcgac attaatcttt gatgttgagt attaaacaaa
    16621 taatctctat aggagactta aaatggcaaa aacaacaaaa gtccaaggca cgaaatttag
    16681 aattagcgtt ggacgtgagg ctcaaaaagc aattaccgca attaacttaa atactgctac
    16741 tctaacgatt gcatcctcag gctataaaaa aggtgatgca atcgagctta caggttgtgg
    16801 tcaattagat ggtatctacc cggtgttatc agtaactacc gatcaggtaa aattatgcga
    16861 agaagtgaac tggacaggta aagatttacc agctaattac accaaagcca aggcggcact
    16921 agtgcaatac tcggagcaat tctgcgcggt aaaaaacatt gaaaaatctg atgatacttt
    16981 gagtaccgaa gatgtaacaa cggtttgttc agagggcaca gaaaccgagc cgggtgaaat
    17041 tgaatttggt tcgatcaaac tcagtttctt ccacaagcct actaccgaaa tgcaaacccg
    17101 attgcgcaaa ctcttttacg acaaatccac ctttgcctac aaactggaac taccggataa
    17161 ccatggcacg acctatggcg aaggctttat tgaggccggc aacggtttct cgggcgaagt
    17221 taagggtaag tatgaaggct ctgtatctat taagccaagc aaacgcgact acttattagt
    17281 ttaattgcaa aaaaaccata aaattcgacc gtttgtagaa ataacaagcg gtctttttat
    17341 atcagaattt tacaaaagga acgaacaatg acactgcgtg agaaactcct tgctaataaa
    17401 ccaaaattgc aacctatcga aatcaacggc gagacctact acctgcgtga agctactgtt
    17461 ggtgatatga acaagcagat ttttgaaacc cgaagctggc tcattcaaca agctgaacaa
    17521 gaaaatgttg aattaccggc agaagatgat gaaacctttg atgaggctct caaccgtttt
    17581 ggcgaaaaat accgccttgc tcaatcggtt gcctaccgct tatgtgatga aaacggtgta
    17641 ttactgttta atcctctcga cattaacgat ctgaatgcca ttgccgaatt agacagtaaa
    17701 gtgattatcg actttaacca agccgtgtcc gccccaaaag actcagcgag cgaagaaagt
    17761 tccaaataac cctttcgctc gcactgggta aaacacttga agaaatcgaa caaatgcccg
    17821 aacgccactt tgccgaatat cggcttttct accaagaaca gcctttcggc ttatggcgag
    17881 acgattaccg caccgcccaa cttgcccact taaccgcaat gttcaaccgt gatccgaaag
    17941 gcaaagctcc aaaattagcg gagtttatgc cgttttttaa tcggacagat gaggtggagg
    18001 aaatagaaga tgatggtgtg gcagattatt tggcgaaaag gtaaagaaaa cagcttgaaa
    18061 ttccttgctc ttggatatat atttcctcaa agagaaggag ggaagtatgt ttgaagcact
    18121 agaccgtttt atgattaata cgattaaaat tgcaattatt gtatttttgg tcgcttgcat
    18181 ctttattagt atgccgaact ggtggatagc ttctatctta gtacttttcc cgactgccta
    18241 tttgcttgat aaatactggt ggcgaaatac cgaaatgtat cgccgcaaaa tggataaatt
    18301 agttaaagag atgaaaaata atcgtcaaat ctaaattaac atcaattcta gcccgcttta
    18361 tgcgggcttt ttatttgggg tgaaaaagtg agtagctcaa acagattaac tattacctta
    18421 gatgtagaaa attctaagtt actagctgga ttcgcacaat ctgatacagt agcaaataag
    18481 tttgctcgca gcttcattgt gaatatggat caggctcagc aaaaagcacg tcaatttgct
    18541 gatagatcga caaaatatct aaaaaatatt gaacaagcag caaataatct taatagctct
    18601 gtatcaaaag aacgtttttg gcaaattgca aatcggttgc aattcccgac aagtaaagtg
    18661 attagttatg ccaatagcta caccgaactc ggtaataaac tcaaattagt caccgaaagt
    18721 gaatcccaac acgcccgagc aatggcggcg gtgtatgata tttcgctcaa aaccgcccaa
    18781 tctacacagg caacctcttc ggtttatcag acctttgctc aaaatgcaaa acagctcggt
    18841 atttctcaaa acgatgtggc aaaactaacg gaaacggtag ccaaatcggt tgctatttcc
    18901 ggtgcaagtt cggcaaccgc ttcaaacgcc ctagtgcaat tcagccaatc gttgttaatg
    18961 ggtaagctca aagcccaaga atttaactca ttgatgaccc aaacaccgtc tgttattcag
    19021 gcgattgcca aagggttagg cattaccact gccgaactta aagcaatggt ggataacggt
    19081 gagatgtctg ctgaaaaaat gattgagggg ctaaaaaaag cagagagtta tgtaaacgga
    19141 cagtacgcca aaacaaccac tacaattagt ggagcaatgc aaaatctttc aactgcggct
    19201 gaaaagtggg ttggcgaaat ggatagtacc gttggtgcgt cacagaaagc cgttgccgtt
    19261 ctgaatgcgt taaatgctca ttttgatacg ttcgctaaaa cagcacttgt cgcaggtggt
    19321 gcatttgtgc ttttcaaagg ctatatgagt gtacgcccat ttatcgaggc tagagcggcg
    19381 atttatgaga atcgaaaagc aattattgcg gaaaatcagg ctcgccaaca aacgatagct
    19441 actcgtcagc aggaattggt tgtactctct caacaaaccg ccttaaaact ggaacaaaca
    19501 ttacaaacct acaatcaaaa taaagccgaa ttagaggcga ttgcggttaa acagcagaaa
    19561 atcacaaaag aacgagaaga aattgctgtt gctattcaag cggcggcaac tcgtacggaa
    19621 agattagcgt tatctgctga actgcaagcg ctagatcaga aagaaatcgc tctcgggcaa
    19681 cagaaaatag cggttgaacg ccagcaaatt gcaacaaaac aggctctcaa agtggcgtat
    19741 gctgaaaatg cagttgctca aagtgcaatg aatgcaggta tggtcactgc aaccacaaca
    19801 accagagcga tgaatggggt tttagcggct gcaagagctg aattgaactt actgaaagca
    19861 gctgcactgt ctaacccatt aatgactctt gcaatggtgg caacgactgc cggtactgcc
    19921 ctttggggat tttttgaaag tcaaaaagca gctcgtgaag aagccctccg ctacgcggac
    19981 agcttagact ccgttcgagc caatatcgaa aaaatgacta aggctcaaac tgatgcagaa
    20041 atggttaaat tgtctcgttc tatcaaagaa caggaaacag cattagctag tctgaaacaa
    20101 cgccaagctg aacttacacg agaaatcgag caaggagcta cggttttaga tgatgccttt
    20161 ggtgccggtg tccgtttcga aaaaagtgcg aaagagttag cgaaagcaca agaagagtta
    20221 aagttagtta ctgctgatgt tgaaaaagca caaaatacat taaatgccag tttagaagct
    20281 caagaagcct tacaagctca cgttccaatc gctaacctac gagaggaatt tgtcaaatta
    20341 tatcctcaca ttgacgaaag ccaaatcaaa gttgatggct taaatattgc tatcggtaat
    20401 tttaccgtca aatcaccgga gatggttatt gccgctaata acattgctaa tgctcttggt
    20461 ggcgttgccg gagaggctat gagagcggct attctagtcg ctaatctgtc aagtatgaag
    20521 ctggatgcta acggtgctgc aattatcgat cctaaacatc ttgaaaaaat cgagcaaatt
    20581 gaacgtaaca atgcgattgc ttcggctaaa ggcaaagata aaatcgcatt gcaagtaaaa
    20641 gacgctctga ttaaatcagg tatgaaagag ggcgatgccg gatacgagcg actaaaagca
    20701 gcatatgagc aacaatttac gttgcaaaat gcccctaagc gcggcggaaa accgaagaaa
    20761 accaaagccg aacgctctgc cgaaagttac caaaaccaag tagcggagat gaccaatcgt
    20821 ttatccggct taaagataga taaagaagat ctcgtaaaat atggtggcat ttctcaatat
    20881 caggaagtgc gtaaacttac tgaagatatt gctctgaatg ctgaaaaata taaaaactac
    20941 ggcactgaag gcgtggctaa actcaaagag ctggctgcta aaattgattc tgaacaacag
    21001 cttttagaca ttgagcaatt ccaagtcgat cacgataaaa agctagaggc aatgcaattc
    21061 gagctggatt tactgggaaa aactcgtact gaaaaagaat tgttacaatt taacaatcaa
    21121 ttagatttag aggccgagcg gctgaaaatt aatatgacga aagagaatat tgctctttta
    21181 gatcagaaaa ttgccaaaat taaggaagaa tacgagctac atcagaaaaa gcaggaagaa
    21241 actcgtgctt ctgtaatgat gggaataaaa gaaggctgga acaatattga aaccgatgtc
    21301 agcaatgtgg cggcaaatgt tgccaatatt actcaaaata cctttgacgg tatggcggat
    21361 agcttgacta attttgtgat gacgggtaaa gcggattttc gtagttttgc taattccata
    21421 ttatctgatc ttagcaaaat gattatcaaa atgatgttat ttaatgctat caaacaaggt
    21481 gcaactatgt tgtttggcta ccaaggaccg actacaacgg taaatggtaa tgctgtttat
    21541 ggattaggta cttgggcaac aggtggctac acaggcaatg gcggcaaata caccccagcg
    21601 ggtatcgtcc acaaagggga atacgtcatc accaaagagg caacctctcg tcttggtttg
    21661 gattatttga attatcttaa ctatggcaga cgtggttttg cctctggcgg tggtgtcgct
    21721 gtaccaagag taccgtcatc atcttatcag ccgaaatcgg ctcaaagtag tatcagcgta
    21781 caggtcatca ataatggtga gccggtagat gccaaagtca gccaaaaaca gcaaggcgag
    21841 caaacgcaaa ttactgttga gttaatgcgt aaaattgccc gtcaggaagc gaacggaatg
    21901 attcagaaca actttagagc cggaggagta tttgcctaat ggaaacatta caatggtgtg
    21961 ttcgcaccga tttagcggtt gaaaataccc ctgaaataat ggaagtcaaa ttcggcgatg
    22021 gttataccca acgctcgccc aaaggcttaa acaatttatt acgagcctat acaggcacaa
    22081 tcaaagtgaa aaagggggaa catttagcgg tggaggcatt ttttgccaaa catcgtggtg
    22141 tttccccttt ttactttaaa gatccgtaca cacaacaaaa caaaaaggta gtgtgtagca
    22201 gttggcccgc aaaaatggtt ggcttaaacc attgggaatt tagtgtaacc tttaaggaag
    22261 taccctaatg cctgttagca tatccaatca aatgaaactc gaccttgcca aacttgagca
    22321 aaatgcaatg cttgatttgt acgaggtaga tttacgcaca ttaaaggata aaagcggtaa
    22381 tgctggctca gtttaccgct tttattcggg gttaaatgaa ctcaaaacaa gtattgtctg
    22441 gcaagggcgg acttacgatc cgtacccgat tgaggcaagt ggatttgaac gaaacagtag
    22501 cggcccaagc aatcgcccta ctcttacgct atcaaatctg tttggcttag ttacgggtat
    22561 tgctaatcag tttgatgagt gtatcggggc gattgttcgc cgtcatcagg tttacgccca
    22621 atatttagat gccgtgaatt ttgccggggg taatgcgaaa gctgatccaa atcaggaaat
    22681 aatcagtcat tttgtgattg agcagcttac cagcctcacc cgagaaacgg cgacctttac
    22741 gctggcatta ccgattgaaa ctgataatgc caaaatcccc agcagaatta ttatggcgga
    22801 cacctgcact tggatttacc gctcggcgga atgtggttat accggtaagc aatattttga
    22861 tgaaaaagac aaggcaacat cagacccaaa agccgataaa tgcagccatt gcctgaacgg
    22921 ttgtgaattg cgaggcaatc agcgtaattt tggtggattt gtatcggtga ataaattggg
    22981 gtaatgaatg gaattggaac aacaaataat cgactacgcc ctccaacacg aaccgcacga
    23041 aatgtgcggc tttgttgttt ttgacggcaa taaaaatcga tttatccctt gcgaaaacca
    23101 agcggaagac aaagccaatt actttgaaat atccgatctt gattacatca aagcagaaga
    23161 aaaaggcgaa ctggtagcgg tggtacattc acatcccgag ccaaacggca agcctatatt
    23221 atctgtgctt gatcgcaaaa tgcaggtaca gaccggttta gattggtggc tagtgcataa
    23281 tcggcaaatc cataaattcc gcaatgtacc acatttaatc gggcgtgagt ttaaacacgg
    23341 cacaatggat tgttacacgc tctaccgtga tgcctatatg cttgccggtt acgagatgga
    23401 cgaattcgat cgacaagatg actggtggca taccgggcaa aatctttatt tggataatat
    23461 ccaagggcaa ggttttgagc gagtgggaac cccacaaatc ggcgatgtga ttttaatgca
    23521 agtaggtgct gatgtgccga accacgctgc catttacatt ggcgaacaga tggtaatcca
    23581 tcatagcccg aaccgattat ctaagcgtga tttatacgac ggctattggt tacgtcatac
    23641 ccacagcatt tggcggcata aactggcgga taagttagat tttgacggca tattgaatga
    23701 tattgctgta aataactaac aaaaaatgac cgcttgtaac aaaaacaaac cccgaagcgt
    23761 ttgcagcact tcggggtttt tcatatccac ttacctaact aagaggacat aaattaaatg
    23821 aatgataaac gatttacatt caaatttcta ggagttctta tggaagcaat taatattacc
    23881 ccaaaagaaa tcagaaaaac aatgtggaca gccgcaataa ttctcttttt gtttgcgttc
    23941 atctggaaag cccctgaatt tatcactgct attcgttggt ggtaactatg attacagtta
    24001 aattttacgg acacttaaga aaatttggta cagactttaa gcttgaggta aaagacaccg
    24061 ctgaggcaat tcgtgcctta tgtacacaga ttagaggatt gcgtgaggca ttgcgagacg
    24121 gtgtttataa agtccgtatc ggtaagcaat atttagaccc atcagccctt gaaaaagggc
    24181 ttttttattg cttgaagaaa gggcaaacca ttcattttac ccctgttatc aaaggggcga
    24241 aaagcggggg tgttttccaa gctgtgttag gtgtagcctt aattggagcc gcattttttc
    24301 tcgggccggt cggttgggga gttctagggg cttcttccgc aatgatgatg ggagcaatgg
    24361 gggcatcaat gttactagga ggagtttccc aaatgctcac caaaatgcca aaagccccca
    24421 caatgggtaa tgaaacagaa aaagaaagct caacggcttt ttcaaattta aataacttgg
    24481 tggctcaagg caaacctgtg ccgttggctt atggcttaat tcggaccggc tcgcttgtga
    24541 tttcacaggg agttgagacg atgactgtag aggtataatt atgggattat ttagtcgtaa
    24601 gagaaaaaat catactccta aagaagcacc ggaaactggt cgctctaaac agattgtgaa
    24661 tatcgtggag ttactctgcg agggtgaaat tgaaggcttg gtagatggct ttaaatcgat
    24721 ttatcttgac ggcacgaaaa tccaaaacga tgacgacagc tataatttta ataatgtgag
    24781 cggtcagtta aatgtcggta cgcaagatca agaggtatta gaaggttacg acagctcaca
    24841 aaatgaagtg agtgtgggtg tagaagtcaa gaaaaaaaac ggtgcaatcg tccgcaccgt
    24901 tactgatgaa cgcatcagcc gtttgcgttt aacgttgggc gtgcggtcac tattcca
//
LOCUS       DANLXS010000014        23989 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.014, whole genome shotgun sequence.
ACCESSION   DANLXS010000014 DANLXS010000000
VERSION     DANLXS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23989
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.014"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(322..969)
                     /gene="rnt"
                     /locus_tag="PXD71_000463"
     CDS             complement(322..969)
                     /gene="rnt"
                     /locus_tag="PXD71_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDL6290620.1"
                     /translation="MEQTTEQIDYKLLKHRFRGYLPVVIDVETAGLNAQTDALLELAA
                     ITLKMDEQGYLVPDQQCHFHIAPFEGANINPESLKFNGIDIDNPLRGAVAENIAIPEM
                     FKMVRKAMKEQGCQRAVIVAHNAAFDQGFLQAAIKRINAKRDPFHPFAMFDTATLAGF
                     MYGQTVLVKACQVAKIPFDGKQAHSALYDTERTAELFCAMVNRLKDLGGFPPLSD"
     gene            complement(1054..1461)
                     /gene="gloA"
                     /locus_tag="PXD71_000464"
     CDS             complement(1054..1461)
                     /gene="gloA"
                     /locus_tag="PXD71_000464"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL6290621.1"
                     /translation="MRILHTMLRVGDLERSIKFYTDVLGMRLLRRSENEQYKYSLAFL
                     GYADESESAVIELTYNWGVDKYELGTAYGHIALGVDDIYKTIEDVRAAGGKITREPGP
                     VLGGTTVIAFAEDPDGYKIEFIENKDAKAALGN"
     gene            1551..2366
                     /locus_tag="PXD71_000465"
     CDS             1551..2366
                     /locus_tag="PXD71_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290622.1"
                     /translation="MAHDHDSPSKLEISTHAALPMVLMLFIEMALNFYSAPKNSIFIS
                     PYLLAFFVTSLISFLVLWKGEICRGQASRLSFILPFLLIFAVGNFLYSIGFTPKHNPM
                     AVASIAAVFACLIYFRLPEDQTLYNTLMYCGLAISSIGIIQYLAIYWFELPSLFNGIR
                     ANNFAQVLLGILLAGWYLMLAKSRLDVFLKLLVKTALVVLILNYCWTAFVLYQHLQIM
                     PDMPLLSFIIYFAAQFVILLMLAWLLLGKNIKNPTAWTVATLLAILYPFANVV"
     gene            2370..3116
                     /locus_tag="PXD71_000466"
     CDS             2370..3116
                     /locus_tag="PXD71_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290623.1"
                     /translation="MKRALKFFGIATVLSVVAIYYFLMPSHYLATPINWILPKAWKVQ
                     LPQGLNLNWQGSSLAQFQLSYRDCPLLSADKTDLGWIEKNHLSVDKLDIDYRCLALFP
                     QNEHSNSDTSLNALLAVIPDGEATIHALNWKNLPTDFNPRLQRLFEQTAIKLSLSKGE
                     IQATAAQQAVKFAANFANGRLNADLSYQPNEKEQHNLRISAEIEDNFTQLPPTISAEY
                     DWQLSYEIIANKALQKGALFSVGKKMPHKS"
     gene            3302..5077
                     /locus_tag="PXD71_000467"
     CDS             3302..5077
                     /locus_tag="PXD71_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HDL6290624.1"
                     /translation="MPLKTAIRISLLSQNSWGKGNIVISNSDGEITEKGLNLPLRLTG
                     NIKHGNFILYSSVPLDFQGNFDDLSVKFLPSSLLRLTGKERYLTIEDLRFPLAGIKID
                     KHGIHGRLHAILRGQSPDFNNIEFHLDGQAQNFKAGALDFFQDPKDANAVKDSWKWRI
                     WGASILKAFNSKVNLSGRGQWHKQLVRLEELKGDLATVKLADTTISKITLNNTQAIRF
                     NVKKFTLDGAVMLQSPEIAFSYGGVLPKPRVNLAFDGEVEKLRFKGAVNTTELGPLKL
                     FARRQLSQDASQIIGKLYWPEQSAQGFQPLFPFRSQWVINNGTIRGETAFSAGSKNGL
                     IAGGHLAIRNGGLSLPNGEAKGIEFSLPYRYQNGRFHFGAKKPLDVKIAQIKLGDLAL
                     DNASMKLNGYYPYTKAKPLNLNQLSFDLFGGKLNVKRFALPQTELAYLNLERIDFEQI
                     LQFMQYQQIDLKGKANAILPFWLSGKPCYICDGLLTQAETSYLTFTPELLSEMQKSGY
                     TEQILLHLVDKSTINDFRSLINVGPKGDLVLDGKLKLSSNQNKSKVNLNYNHRENLFD
                     LWHLINYGSQFEQKLENYLYQKLER"
     gene            5095..5289
                     /locus_tag="PXD71_000468"
     CDS             5095..5289
                     /locus_tag="PXD71_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDL6290625.1"
                     /translation="MKPATFYFAIFLLNLTACTPKVQLETPSEGITINMNVVVDHRID
                     VKMDEKSRAVITKSENKTKN"
     gene            complement(5286..5759)
                     /gene="folK"
                     /locus_tag="PXD71_000469"
     CDS             complement(5286..5759)
                     /gene="folK"
                     /locus_tag="PXD71_000469"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDL6290626.1"
                     /translation="MQTVYIALGSNLENPLEQLKQAVEKLKKFANEFEISPFYGSKPV
                     GPQDQPDYVNAVAKFSTDLSPTALLDKLQSIENEQGRVRVRRWGERTLDLDIILYGNQ
                     QIQTERLNVPHIEMKNREFVIVPMFDLTPDLVLPTGEKLADIYAQFKDHQMIIFS"
     gene            complement(5962..7293)
                     /gene="pcnB"
                     /locus_tag="PXD71_000470"
     CDS             complement(5962..7293)
                     /gene="pcnB"
                     /locus_tag="PXD71_000470"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDL6290627.1"
                     /translation="MLHKKFAVNASQYRISPKDFPKNAISIVDKLQIAGYEAYIVGGC
                     IRDLLLEKKPKDFDIATNARPEEIQRIFGRQCRLIGRRFRLAHIVYGREIFEVATFRA
                     GHESHRNDKISKVSDEGMLLRDNVYGTLREDAERRDFTVNALYYDIKRNLIFDFFNGI
                     EDLRAGKLSLIGEPTIRYQEDPVRMLRAVRFMAKLDMFLDKATASPIRDMAVLLTNIP
                     AARLFDESLKLLQTGAGLKTYQLLREYELWQFLFPVLPAFFTAKGDSYAERMITKALN
                     STDERIADKLPVNPAFLFAALLWYPLREKMEQLKNEGGFNSYDAMMLAANDVLAESCN
                     AIALHRRHTSVIRDIWALQFQMMKRSGNRPQQTLEHVKFRAGFDLLVMRAEIEGGDLV
                     ELSAWWHEYQFSNEGQRTELLKSVRHLGNVPSEKKKTRRRRTFKKKPKVNG"
     gene            complement(7538..7984)
                     /gene="dksA"
                     /locus_tag="PXD71_000471"
     CDS             complement(7538..7984)
                     /gene="dksA"
                     /locus_tag="PXD71_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDL6290628.1"
                     /translation="MTQAAKTSLGLLALAGVEPYELKKDEEYMNEAQREHFTKILKAW
                     HSQIMEEAERTKSQLQEDMTNFADPNDRASQEEEFSLELRNRDRERKLLKKIEQTLYK
                     IQEDEYGYCETCGVEIGIKRLEARPTADLCIDCKTLAEIREKQLGL"
     gene            complement(8234..9187)
                     /gene="thiB"
                     /locus_tag="PXD71_000472"
     CDS             complement(8234..9187)
                     /gene="thiB"
                     /locus_tag="PXD71_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDL6290629.1"
                     /translation="MKFILSSLALLSASVYANQPTLTVYTYSSFASEYGPAPKLEQNF
                     EKECGCDVKFVPLDDSVTMFNRLRLEGAKTKADVVVGIDNFLQPEAEKSGLFAEFEAK
                     TKLETQQKIFFPYDTGSYAFIYDKEKLKNPPKSLKELVERQDLKVIYQDPRTSSVGRG
                     FLMWVNQVYGDEAPTAWKTLAKHTVTVGKGWSETYGAFLKGESDLVLSYTTSPLYHKW
                     HENTDKYVAAPFEEGHLVQVENAAIVKSSQQKALANQFLEFLHKPESQHIISYHNVMN
                     PVLTDGVDPAFAAMPTYKTIEFTPPTTETIQKWLADWSKSW"
     regulatory      complement(9231..9374)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9658..10644
                     /locus_tag="PXD71_000473"
     CDS             9658..10644
                     /locus_tag="PXD71_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL6290630.1"
                     /translation="MSQLIRTLKGHIRDEIIKKGGWVNAHAHADRAFTMTPEKITVYQ
                     NANLQQKWDLVDEVKRNSSVEDYYRRFSQAIELMISQGVTAFGTFVDIDQVCEDRAII
                     AAHKAREVYKNDIILKFANQTLKGVIEPNAKKWFDIGSEMVDMIGGLPYRDELDYGKG
                     LEAMDILMDKAKSLGKMLHVHVDQFNSPKEKETEQLCDKSIEHGMQGRVVAIHGISIG
                     SHPKEYRKMLYQKMRDSQMMVIACPMAWIDSGRKEDLQPFHNALTPADELIPEGITVA
                     IGTDNICDYMVPLCEGDMWQELSLLSAGCRFTNLEEMANIASINGRKVLGLV"
     gene            complement(10698..11117)
                     /locus_tag="PXD71_000474"
     CDS             complement(10698..11117)
                     /locus_tag="PXD71_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDL6290631.1"
                     /translation="MLGNILGSLASSVLSGNNQSTATQLIQNLLASQGGIEGLIAKFQ
                     QGGLDDVLRTWISSDEDNAPVSGEQISNVFGQENIQNVAQEAGVDAADASDLLAQFLP
                     KIVDTLTPNGNVEDLKNFDANDLLAQAAKGILGNLFK"
     gene            complement(11195..11950)
                     /gene="kdsB"
                     /locus_tag="PXD71_000475"
     CDS             complement(11195..11950)
                     /gene="kdsB"
                     /locus_tag="PXD71_000475"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDL6290632.1"
                     /translation="MKFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQLAGASR
                     VIIATDHPEIEALAQSFGAEVCMTSEKHNSGTERLAEVIEKMQIADSEIIVNIQGDEP
                     LIPPVIVTQVAQNLDAYQVNMATLGVKLATREELFNPNAVKVVADKNGMALYFSRASI
                     PFARDSFPDCDDSFVAAQGYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALW
                     HGEKIHIEPAKEAPQVGVDTPEDLERVRAILAR"
     gene            complement(11960..12913)
                     /gene="trxB"
                     /locus_tag="PXD71_000476"
     CDS             complement(11960..12913)
                     /gene="trxB"
                     /locus_tag="PXD71_000476"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL6290633.1"
                     /translation="MTTKHTKLLILGSGPAGYTAAVYAARANLNPVLVTGMQQGGQLT
                     TTDEIENWPGEFEQITGTELMNKMLKHAEKFETEIVFDHIHSVDLSKRPFTLNGDLYT
                     FTCDALIIATGASAKYLGLPSEEHFKGKGVSACATCDGFFYRNKPVAVVGGGNTAVEE
                     ALYLANLASEVHLIHRRDSFRAEKILLGRLQKRIEEGKIILHTDRTVDEVLGDNMGVT
                     GLRLKSTKEELSEEIKVDGFFVAIGHAPNTAMFEGQLELENGYIKVKSGLEGNATATS
                     VEGVFAAGDVMDHNYRQAITSAGTGCMAALDAERFLDAQEA"
     gene            13080..14150
                     /gene="dhaK"
                     /locus_tag="PXD71_000477"
     CDS             13080..14150
                     /gene="dhaK"
                     /locus_tag="PXD71_000477"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL6290634.1"
                     /translation="MKKLINSIETVLDEQLSGFVKAHSDLTLNTEPVYIYRTTPSNQP
                     KVALISGGGSGHEPMHAGFIGKGMLDGACPGAIFTSPTPDQMFECGLAVDRGEGVLLI
                     IKNYTGDVLNFETATELLADSGVKVATVLVDDDVAVKDSLYTAGRRGVANTVLIEKLL
                     GAAADKGYNLSELAKLGHQLNNNGHSIGIALSACTVPAAGKPSFTLAENEMEFGVGIH
                     GEPGIERRPFENLDSTVKQMFETLIAHGDYQRKIRHWNTQTQQWDEIEESKVALKKGD
                     RVIALVNNLGAVPLSELYAVYDALEKECQTFGLSIERSLVGSYCTSLDMQGISITLLK
                     VDEDILKLWDAPVNTPAMRWGE"
     gene            14171..14797
                     /gene="dhaL"
                     /locus_tag="PXD71_000478"
     CDS             14171..14797
                     /gene="dhaL"
                     /locus_tag="PXD71_000478"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDL6290635.1"
                     /translation="MTISKQQILKWLENCQQVMTEKRDYLTELDTVIGDGDHGLNMQR
                     GFSKALEKAHTVQDKDIGTLLKTVGMTLLSQVGGASGPLYGSFFIKAAQGATGKEQLS
                     FNELLALLQQGAEAIVQRGHAQVGDKTMCDLWLPLLEDLAQADSTQPIAQILLQAVEK
                     AENFANQTAPLMAKKGRASYLGERSIGHKDPGATSSYYIFKALAEALQ"
     gene            14807..15214
                     /gene="dhaM"
                     /locus_tag="PXD71_000479"
     CDS             14807..15214
                     /gene="dhaM"
                     /locus_tag="PXD71_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL6290636.1"
                     /translation="MVNLVIVSHSAKLAEGVGEILAQMAQGGCKIALAGGIEDTSNPI
                     GTDAVKIMQAIESVFSPDGVIVFVDLGSAILSTQTAIDLLEPELAEKVIISYAPLVEG
                     AFAAVVAASAGDSLEAVLNEANEAAALKQEQAN"
     gene            complement(15254..16099)
                     /locus_tag="PXD71_000480"
     CDS             complement(15254..16099)
                     /locus_tag="PXD71_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HDL6290637.1"
                     /translation="MDYFVNVNEQNFSEVWAAAAQVPTVFHFISPREVASLEMDALLR
                     RISDENPNQFLLATVNCDEQQALAAQFRIQQIPTLYLFAGGQPIDAIQGAVSEQELRI
                     RLANILPKEEELKFQQALELLQDQRYTEALPLLKSAWEMTDKKNSDFALLYAETYIAM
                     KSVEEAREILKAIPIQDRDSRWHGLQAQIELLEQASESPELQQLQADYANSKTPEIAI
                     KLANQLHQAHKNEEALTLLFDWLKSDLNVANGEVKSQFLAILSALGTDPIVPKFRRQL
                     YSLLY"
     gene            complement(16143..16469)
                     /gene="glpE"
                     /locus_tag="PXD71_000481"
     CDS             complement(16143..16469)
                     /gene="glpE"
                     /locus_tag="PXD71_000481"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDL6290638.1"
                     /translation="METTFKEISPQQAWELIENEGAVLADVRDARRYVYSHPQDAFHL
                     TNQSYGKFLDEVDYDEPVVVICYHGVSSQSTAQFLIEQGFENVYSVKGGFEAWERNGL
                     PIETSY"
     gene            16620..17078
                     /gene="exbB"
                     /locus_tag="PXD71_000482"
     CDS             16620..17078
                     /gene="exbB"
                     /locus_tag="PXD71_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HDL6290639.1"
                     /translation="MEQLFQFLEHHTDFVILGLLGLLSVICLWKAIERIMFYSKVDIS
                     KYDTLEELDIDLTHNLTTISTIGSNAPYIGLLGTVVGILLTFYHLGQSGGDIDAASIM
                     VNLSLALKATAVGILVAIIAMVFYNGFGRKVEENKLKWLALQSKKPHQAV"
     gene            17115..17552
                     /gene="exbD"
                     /locus_tag="PXD71_000483"
     CDS             17115..17552
                     /gene="exbD"
                     /locus_tag="PXD71_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDL6290640.1"
                     /translation="MKKFDEINIIPFIDIMLVLLAIVLVTASFISQGKIQVNVPKAST
                     TQPMKADDLAKLLTITENNEFFFNDQAITKEQLIAEVATWDKSQKVSLKVDGAVAFEK
                     FVELTDILSANEIKNVAIITKKETAPAPSSTPGSPAQVPAVAP"
     gene            17568..18479
                     /locus_tag="PXD71_000484"
     CDS             17568..18479
                     /locus_tag="PXD71_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDL6290641.1"
                     /translation="MKKKHSRIGLLASLAIHTALIGGTFAWLHNHKVEKIAEQNSLSM
                     EMVAALLEQPQVAVAPDPITEEVKEIEPEKAEVPPEPKLTPEPTPEPEAIPDPTLNPK
                     KEKPKEEKRKERHHHKHKHRHKEKREELQEQEKPKDKPKERPKHHHKHIKALERGPEI
                     KQGIVAKAIPNAAEGEKVVAGVVGGQKNGSPNSTSTTGSANTGASSGNASELAAYKVA
                     LQRALQRKANNSYPQREKMMRKTGTVTIKFTITSSGSITNVSVLNSSGNSNLDNAAVR
                     SAEGLSMPPPPAGFPPTVTVPVRFALE"
     gene            complement(18548..19429)
                     /gene="cysZ"
                     /locus_tag="PXD71_000485"
     CDS             complement(18548..19429)
                     /gene="cysZ"
                     /locus_tag="PXD71_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDL6290642.1"
                     /translation="MSSVFSEPQNQVGLGFHYFIYGWRLLLQRQFLPFVIFPILINTV
                     LMVGLIWGFFANIGGLLDMLLPSWLEWLSFVLIPLIFLMILVGFYFAFTTLANFVAAP
                     FNALLAEKVELQLTGEPLGDMSFGAMLKDVPRMLKREWQKMMYSIPRLIALFVLGFLP
                     VVGQTVAPVLTFIFGAWLIAIQYCDYPFDNHKISFQRMRNALSQSRTLNFTFGALVSL
                     FTMIPFVNLVVMPVAVCGATALWVKEYRNFFLNNQTGEFSKADYTFTKVNSTSLSTET
                     RTGEVSPNVRNGDIRKQ"
     gene            19541..20443
                     /gene="zipA"
                     /locus_tag="PXD71_000486"
     CDS             19541..20443
                     /gene="zipA"
                     /locus_tag="PXD71_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDL6290643.1"
                     /translation="MELHIIFFILAGLLIAVLIGFSLWSARREKSNIFSNTFSTRPIA
                     VPPQNNKMSNSIPTSLQPQNEDYQAMSVPEENPQQIQQEVESSLQNIKISLPGGNPQQ
                     QPEQIQPIQSVAEPTVSTYGQKISNQQHIQYNTPDFANPMVNTVVEETPQAVAIEPQE
                     TQSAEEENPFVTLYLYAPQNEAFRGDFVVHYLEEVGLRFGEHQIFHRHQHIDDSLSPI
                     IFSAANLMNPGTFDLNNISNFATAGLVLFMQLPTEGNDVANMKLLISNAEHLAASLGG
                     FLYDDNQQPFGEESRQRYMQRVMH"
     gene            20508..22565
                     /gene="ligA"
                     /locus_tag="PXD71_000487"
     CDS             20508..22565
                     /gene="ligA"
                     /locus_tag="PXD71_000487"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL6290644.1"
                     /translation="MSLFNQTSLFEENATPELVEKIETIRQTLRQYEYEYHVLDNPSV
                     PDAEYDRLMNELKNLEWQHPELISADSPTQRVGAKPLAGFAQVTHEIPMLSLDNAFSD
                     EDLDGFLRRMESYITASPSQLEFCCEPKLDGLAVSILYENGILTRAATRGDGTTGEEI
                     TANIRTIRNIPLKLNTTNPPARLEVRGEVFMPQSGFNELNEKALAKGEKTFANPRNAA
                     AGSLRQLDPKITRQRPLVLNAYGIGVYESDDELPATHFERLQWLKSLGIPVNNEIRLV
                     QGKAALLAYYAEIQAKRPTLGYDIDGTVLKVNDIALQEQLGFISRSSRWAIAYKFPAQ
                     EEMTILKEVDFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVI
                     IRRAGDVIPQITGVMLERRPENAKKIEFPTACPICHSAVTRVEGEAVARCTGGLFCEA
                     QRKEALKHFVSRKAMDIDGVGEKLIEQLMARELIETPADLFKLDHTTLMRLERMGEKS
                     AQNALNSIEKAKNTTLPRFLFSLGIREVGEATALNLANHFGSLEAIRQATFEQLKEVQ
                     DVGEVVANRIVSFWQEPHNVEVVEDLIAQGVRWENVVKAEIVDNPLKDKNVVLTGTLT
                     QLTRDEAKALLQSLGCKVSGSVSSKTDYLIAGEKAGSKLAKAQELGIQILSEQQFIEL
                     TQH"
     gene            complement(22628..23896)
                     /locus_tag="PXD71_000488"
     CDS             complement(22628..23896)
                     /locus_tag="PXD71_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL6290645.1"
                     /translation="MTQQIEHSTWQSKFAAMGPGILMASAAVGGSHIIASTQSGAIYG
                     WQLAIIILLANLFKYPFFRFGVQYTLASGKTLLEGYKEKGKFYLIIFFILNVFAAMIN
                     TAAVAIVSAAILKFIFSALGIPLDLSIPMAGTIVIVVTWGILLLGQYKFLDTLSKWIM
                     IALTISTVSAVIIAALKGGVEIAPDFVEPSPWNLAALVFIVALMGWMPAPIEISAINS
                     MWVIAKKRLSKVNYEDGIFDFNVGYIGTAILAIVFLALGALVQYGSGEKVEQAGVAYI
                     AQLIKMYAFAIGDWSKLLIAFIAFMCMFGTTITVIDGYSRANAEALRLISGRKQHSQT
                     ELSIWMTVTALIGILIISQFMSDVAKMLQFAMISSFVSTPVFAWLNFSLVLKGEHRAK
                     GWLFWLAVIGLIYLTSFTLLFLAQQFKLIG"
BASE COUNT         7270 a         5211 c         4739 g         6769 t
ORIGIN      
        1 tttcaaggtg gaaacgcttg tttttagatt agataaaaaa taaggagggt cgaaatagcg
       61 ggtatacaga aagtgcttat agccaaaaaa tagtctattg ttattgaata caagcggtct
      121 attttgtcac atttttgcca aatttggtta aaaaaccaac cgcttgtaat cttaactgaa
      181 gatagatagt tttttatttt ttctacaact ctacagggct ggatttattg cagaatggta
      241 cttgccacta actatcatca ctcccaggat aatttataga aacaatacga gaaattgtgt
      301 taataaatga aatgaataac gttaatccga taatggtggg aaaccaccta aatcttttaa
      361 acgattgacc attgcacaga aaagttctgc cgtgcgctcg gtatcataca acgcagaatg
      421 cgcttgctta ccgtcaaacg ggatttttgc cacctggcag gcttttacca acacggtttg
      481 cccatacata aaaccggcaa gggtggcggt gtcaaacatc gcgaaagggt gaaagggatc
      541 tcgcttggca ttaattcgtt taatcgcagc ctgtaaaaag ccctgatcga acgctgcatt
      601 gtgggcaaca atcaccgccc gttggcagcc ttgctccttc attgctttgc gaaccatttt
      661 gaacatttcc ggaatcgcaa tattctccgc aaccgctcct cttagagggt tgtcaatatc
      721 aatgccgttg aattttaatg actcagggtt aatattcgcc ccttcaaatg gcgcaatatg
      781 aaaatggcat tgttgatccg gcactagata accttgctcg tccattttta aagtaattgc
      841 cgccaattcc aataaggcat cagtttgggc atttaagcct gctgtttcca catcaatcac
      901 aaccggtaaa tagccacgaa aacgatgctt taataattta taatctattt gttccgttgt
      961 ctgttccatt tatctatcaa cctaaaatac aagcggtctg atttgcaaaa ttttttacaa
     1021 atccgaccgc ttgaatattc atattcgcaa aaattagttt cctaacgctg ctttcgcatc
     1081 tttattctca ataaattcga ttttataacc gtccggatct tcagcaaagg caatcacagt
     1141 tgttccaccc aataccgggc ccggctcacg ggtaatttta ccgcctgccg cacgcacatc
     1201 ttcaatggtt ttgtaaatat catctacgcc taacgcaata tggccataag ccgtgcctag
     1261 ctcatattta tccacacccc agttataagt cagctcaatc accgcacttt cgctttcatc
     1321 ggcatagcct aaaaatgcta acgaatactt atactgctcg ttctcgcttc tacgtaacag
     1381 acgcatacct aacacatcgg tataaaactt aattgagcgc tctaaatctc caactcgtaa
     1441 cattgtatgt aaaattcgca tattttcttc cccctgaagt taaaaaacga cccctattat
     1501 ggcataatct tggcttaaaa tcattttctt tatacctttt gggattatct atggctcacg
     1561 atcacgattc gccgtcaaaa cttgaaattt caacccacgc tgcactgcca atggtattaa
     1621 tgctgtttat tgaaatggct cttaacttct actctgcacc gaaaaattca atttttattt
     1681 ctccttactt attggcgttt ttcgtgacct cattaatcag ttttttagtg ctatggaaag
     1741 gcgaaatctg ccgagggcaa gccagccgtt taagctttat tttacccttt ttacttattt
     1801 tcgccgtagg gaatttcctt tattccatag gctttacgcc aaagcataac ccaatggcgg
     1861 tggcaagcat tgctgcagtt tttgcctgct taatctactt ccgcctgccg gaagatcaaa
     1921 ccttatataa cacattgatg tattgtggtt tggctatatc aagtattggt attatccaat
     1981 atttagccat ttattggttt gaactgcctt cattgtttaa cgggattcga gccaacaatt
     2041 tcgcccaagt gctgttaggc attttattag ccggctggta tttaatgtta gcgaaaagcc
     2101 gtttagatgt tttcctcaaa ctgttggtga aaaccgccct tgtggtgctg attctcaatt
     2161 attgctggac agcttttgtg ctttatcaac atctacaaat tatgccggat atgccattgc
     2221 tttcatttat catctatttt gcagcacaat ttgtgatttt attgatgttg gcgtggttat
     2281 tacttggaaa aaacatcaaa aatccgaccg cttggacagt tgctacgctg ttagcgattt
     2341 tatacccttt cgctaatgta gtctgattta tgaaacgggc attaaaattt ttcggcattg
     2401 caaccgttct atctgttgtg gcaatctact attttttaat gcccagccac tatcttgcca
     2461 cgccgattaa ctggattttg ccgaaagcgt ggaaagttca actcccacaa ggcttaaacc
     2521 tgaattggca aggctcatca cttgctcaat ttcaactctc ttatcgagat tgcccgctac
     2581 taagtgccga taaaaccgat ttgggttgga ttgagaaaaa ccatctttct gtcgataaat
     2641 tggatattga ttaccgctgc cttgccttgt ttccacaaaa tgaacacagc aattccgaca
     2701 cttcactcaa cgcattgctg gcggtcattc ctgatggcga agcgactatc cacgccctta
     2761 actggaaaaa tttgcctacc gatttcaacc caagacttca gcggcttttt gagcaaacag
     2821 ctattaaact ctcactttca aaaggtgaaa ttcaggcaac tgccgctcaa caagcggtca
     2881 aatttgcagc aaattttgca aatgggcggt tgaatgccga tttatcatat caaccgaatg
     2941 aaaaagaaca gcataatctt aggatttcag cagaaattga ggacaatttt acccaactgc
     3001 cgcccactat ttccgctgaa tatgattggc aattatctta tgaaatcatc gccaataaag
     3061 ccttgcaaaa gggcgctcta ttctcagttg gcaaaaaaat gccgcacaaa agttagaagg
     3121 caactggcag gttgaacttg ccgaaagtga aaataacaaa ctggatttcc cttttctgtt
     3181 tgatggggaa agcctagaaa ttcaacaagg gcggtttgat tggcacttta cccaacaatt
     3241 tccactgcaa gggtttgtta gcaccaagct cacaccgaaa tcattcaacc aaaacggctt
     3301 tttgccactc aaaacggcga ttcgtatcag cctgctttcc caaaacagtt ggggtaaagg
     3361 caatattgta atctccaatt cagacggaga aataaccgaa aaagggctga atttaccgct
     3421 tcgtttgacg ggtaatatca aacacggcaa ttttattctt tacagtagcg tgccactgga
     3481 ttttcaaggt aattttgacg atttaagcgt aaaatttttg cctagctccc tacttcgttt
     3541 gaccggaaaa gagcggtatt taaccattga agatttacgt tttccacttg ccggaattaa
     3601 aattgacaag cacggtattc acggacgact gcacgcaatt ttaagagggc aaagccctga
     3661 ttttaacaat atcgagttcc atttagacgg acaagcacaa aattttaagg ctggagcctt
     3721 agattttttc caagatccta aagatgctaa cgcagtaaaa gacagttgga aatggcgaat
     3781 ttggggagca tctatcctca aagccttcaa cagtaaagtg aatttaagcg gcagagggca
     3841 atggcataaa cagcttgtgc ggttggaaga actcaaaggc gatttagcca ccgtaaagtt
     3901 agcagataca accatttcta aaatcacgct taacaatacg caagctattc gatttaatgt
     3961 caaaaaattc acccttgacg gagcggtaat gttgcaatca cctgagattg ccttcagtta
     4021 tggcggtgtg ctacccaaac cacgagtgaa tctcgccttt gatggtgaag tcgaaaaact
     4081 acgttttaaa ggagcggtga ataccaccga actcggccca ctcaaactgt tcgcccgccg
     4141 ccaattaagc caagatgcca gccaaattat cggcaaactt tattggccgg agcaatctgc
     4201 tcaaggcttc cagccgcttt ttccgtttcg tagccaatgg gtgatcaaca acggtacaat
     4261 tcggggcgaa acggcgttta gtgccggcag taaaaacggg ctgattgccg gtgggcattt
     4321 agccattcgt aacggcggtt tatctttacc taatggcgaa gcaaaaggga ttgaattttc
     4381 gcttccctac cgctaccaaa acggacgctt tcatttcgga gccaaaaaac cgcttgatgt
     4441 aaaaattgcc caaatcaaac tgggggattt agcattagat aacgcttcaa tgaaactcaa
     4501 cggttactac ccttatacca aagctaagcc attgaattta aatcaattat cctttgattt
     4561 gtttggcggt aaactgaatg taaaacgctt tgccttacca caaaccgaac tggcttacct
     4621 gaatttagag cggattgact ttgagcaaat tttgcaattt atgcaatatc agcaaattga
     4681 tctcaaaggc aaagccaacg ctatcttgcc gttttggtta agcggaaaac cttgctatat
     4741 ttgcgatggc ttactgacac aggcggaaac ttcctattta acctttaccc ctgaattgtt
     4801 gtcagaaatg cagaaaagtg gttacaccga gcaaattttg ttacatttag tagataaaag
     4861 cacgattaac gattttcgca gtctgattaa tgtcgggccg aaaggcgatt tagtattaga
     4921 cggaaaatta aaattatctt ccaatcaaaa taaatcaaaa gtgaatttaa actataatca
     4981 ccgagaaaat ctgtttgatt tgtggcactt aattaactat ggctcacaat ttgaacaaaa
     5041 attagaaaat tacctctatc aaaaactgga acgatgataa agaatggaat aaatatgaaa
     5101 ccggccacat tttattttgc aatattttta ctgaatttga ccgcttgtac gccgaaagta
     5161 cagcttgaaa cgccaagtga aggcattacg attaatatga atgtggtcgt tgatcaccgc
     5221 attgatgtga agatggacga gaaatcccga gcggtgatca ccaaaagtga gaacaaaacg
     5281 aaaaattagc tgaagataat catttgatga tctttaaatt gtgcataaat atcggctaat
     5341 ttttcccctg tcggtagtac taaatcaggc gttaaatcaa acatcggcac aatcacaaat
     5401 tcccgatttt tcatctcgat atgcggcacg ttcaaacgct cggtttgaat ttgctgattg
     5461 ccgtacaaaa taatatccag atctaaagtt ctctcccccc aacggcgtac acgcactctt
     5521 ccctgctcat tttcaatgct ttgcaattta tccagcaatg cagtgggcga taggtcggta
     5581 ctaaattttg ctaccgcatt gacataatca ggctgatctt gcggtccgac aggctttgag
     5641 ccgtagaacg ggctgatttc aaactcattt gcaaattttt ttaatttttc gaccgcttgt
     5701 ttaagctgct caagcggatt ctcaaggttt gatcctaagg cgatatagac ggtttgcata
     5761 aaatatactt acaaatataa caataaactc tctccccttg tgggagagag accgcttatg
     5821 agcgttcagc gaaataagca gagagagggg acaagcggta acttttatta aattttttac
     5881 acatttcccc tctccctgat ttttcttctg aacgaagaaa aatcgctccc tctcccacaa
     5941 ggggagaggg aaatatatta attatccatt tactttcggt tttttcttaa aggttctacg
     6001 gcggcgtgtt ttctttttct cgctaggcac attgcctaaa tgacgaaccg atttcagcaa
     6061 ttcagtacgc tgaccttcat tgctaaattg gtattcgtgc caccaagcgg aaagttcgac
     6121 caaatcgccg ccttcaattt ctgcacgcat cactaataaa tcaaaccctg cacggaattt
     6181 gacgtgttca agggtttgtt gcggtcggtt gccacttcgt ttcatcatct gaaattgcaa
     6241 tgcccaaata tctcgaatga ctgaagtgtg tctgcggtgc aacgcaatcg cgttgcaact
     6301 ttccgccagc acatcatttg ctgccagcat cattgcatcg tagctgttga agccaccttc
     6361 gtttttaagc tgctccattt tctcccggag cggataccaa agtagtgcag caaataaaaa
     6421 cgcaggattt accggtaatt tatcggcaat tcgctcatca gttgaattta acgctttggt
     6481 aatcattctt tcggcatagg aatcaccttt ggcagtaaaa aatgccggca ataccggaaa
     6541 caagaattgc catagttcat attcacgcaa aagctgataa gttttcagcc ctgcaccggt
     6601 ttgtaataat ttgagtgatt cgtcaaacaa acgggctgcc ggaatgttgg ttaacaacac
     6661 cgccatatcc cgaattgggg aggccgtggc tttatccaag aacatatcca acttcgccat
     6721 aaaacgaacg gctcgcaaca ttctcaccgg atcttcttgg taacgaatag taggctctcc
     6781 gataagcgaa agtttacctg ctcgtaagtc ttcaataccg ttgaagaaat caaaaataag
     6841 attgcgtttt atatcgtaat ataaggcgtt tacggtgaaa tcacggcgct ctgcatcttc
     6901 acggagcgtg ccgtaaacat tatcacgcag tagcattcct tcatcgctga ctttagaaat
     6961 tttgtcattg cgatgagatt cgtgtcctgc acggaaagtc gccacttcaa aaatttcacg
     7021 cccgtaaaca atgtgtgcca aacggaaacg acgtccgatt aatcggcatt gcctaccaaa
     7081 aatacgctga atttcttcag gtctcgcatt agtcgcaata tcgaaatctt taggcttttt
     7141 ctccaagagt agatctcgaa tacagccacc cacaatataa gcttcataac cggcaatctg
     7201 caatttatcg acaatcgaaa tcgcattttt aggaaaatcc ttcgggctaa ttctatattg
     7261 gctggcattc accgcaaatt tcttgtgtaa caaatgggag ggaggattat cttgttgttt
     7321 cttcccacct ctataagtct tttggtccgt taagttcggg gctgggcgtt tagaacggga
     7381 agttttgggt tctctttccg ctcttttttc actctctacc ttttcatttt tctgagccga
     7441 ctttttaggc ttggaaaata atgatttaat ataattaaaa ataatacacc tctaaatagt
     7501 aaatgcctct taaaaaagag gcaccctgta aagttgatta taagcctagc tgcttttctc
     7561 gaatttcagc taaagtttta cagtcaatac ataaatcagc tgttgggcgt gcttctaaac
     7621 gtttaatccc aatttctacg ccacaggttt cgcaataacc atattcatct tcttggattt
     7681 tatacaaggt ttgctcaatt tttttcaata atttgcgttc acgatcacgg ttacgaagct
     7741 ctaaactgaa ctcttcttct tgactggctc ggtcgttcgg atcggcaaag ttagtcatat
     7801 cttcttgaag ctggcttttg gtacgctccg cttcttccat aatttgactg tgccacgctt
     7861 tcagaatttt ggtgaaatgc tcacgttgag cttcattcat atattcttcg tcttttttaa
     7921 gctcataagg ctcaacgcct gctaatgcta ataaaccgag tgaggtttta gccgcttgag
     7981 tcatagatat ttactccttg ttccctgttt attggaagtt ttattgttaa aattattgcc
     8041 cgaatacaga ctaacttcct tgtaaccgca ctatacgaaa aaatcaacat cttcgggttt
     8101 taaataatgg cggctataaa taacacaaaa acgactcatt aatcaaatga atttgcaaaa
     8161 attttgcatt caaattgatt tgattgcaca aaaaaatagc cgaacatcca ttcggctatt
     8221 tttcaaacac tttttaccag ctctttgacc aatcggctaa ccatttttgg atagtttcag
     8281 tagtcggtgg cgtaaattca atggttttat aggttggcat tgccgcaaaa gccggatcaa
     8341 cgccgtcggt taaaaccgga ttcattacgt tgtggtaaga aataatatgt tgcgattccg
     8401 gtttgtgcaa aaattcaaga aattgatttg ccaatgcttt ttgttggctg cttttgacaa
     8461 tagccgcatt ttctacttgc accaaatgcc cttcctcaaa cggagcagcc acatatttat
     8521 cggtgttttc gtgccactta tgatataaag gcgatgtagt atagcttagt accaaatccg
     8581 actcgccttt taagaacgct ccataagttt cagaccaacc tttgcctacc gttaccgtat
     8641 gttttgccaa tgttttccaa gcagttggag cttcatcgcc atacacttga tttacccaca
     8701 ttaagaagcc ccgcccaacg ctgctggtac gtgggtcttg gtaaatcact tttaaatctt
     8761 ggcgttccac caactctttt aaactttttg gtggattttt cagcttctct ttatcgtaaa
     8821 taaaagcata agaacccgtg tcataaggga agaaaatttt ctgttgggtt tctaatttgg
     8881 tctttgcttc aaactcggca aacaagccac ttttttcggc ttcaggttgc aggaaattat
     8941 caataccgac caccacatca gctttggttt ttgctccctc caaacgcaag cggttgaaca
     9001 tcgttacgct atcgtcaagc ggaacaaatt tgacatcaca gccacactct ttctcaaaat
     9061 tctgctctaa tttaggagca ggtccatatt ccgaagcaaa cgagctgtaa gtgtaaacgg
     9121 ttaaggtcgg ttgattggca taaacacttg cacttaataa cgcaagtgaa gagaggataa
     9181 atttcataac aattccttct aaattaaatg aatagaagga tttaagaaag gcaagttgct
     9241 atctcaaatc cctacgctga tattaatcag atcaggttct atgggtattt ctcagccaca
     9301 agcggttaaa tttcggcaaa aatttgcaaa tttttacgca aaaacaaccg cttgttgcac
     9361 cccaatgaga acggcttcat tgtaaaaagt ttgcaggcag atttcaacca aagcaaacgt
     9421 ttgctatttg tattaaaaat ccgtatacta ccgccctagt ccaaacaagg aactagaaat
     9481 gaattgcaca acatcggtta aatcaaattc ccaacagcta cgcttcaact ttagaggcgt
     9541 agctgttttt ctattcattt caaaccattc aaaatcgctg ttatagcgat tttttttcgc
     9601 acgcttgcaa ttaccgcaag cggttgagcc acattaacag agagaaaagg aataaaaatg
     9661 agccaattga ttcgtaccct aaaaggacat attcgtgatg aaatcatcaa aaaaggcggt
     9721 tgggtaaatg cacacgccca cgcagatcgt gcatttacga tgacgcctga aaaaatcacc
     9781 gtttaccaaa atgcgaatct acagcaaaaa tgggatttgg tcgatgaggt aaaacgtaat
     9841 tcaagcgtag aagattacta ccgccgcttt tcccaagcga ttgaattgat gatttcacaa
     9901 ggggtaacgg catttggtac ttttgtggat atcgaccaag tatgtgaaga cagagccatt
     9961 attgccgctc acaaagcccg tgaagtatat aaaaacgaca tcatcttgaa atttgctaac
    10021 caaacgttga aaggcgtgat tgaacccaat gcgaaaaagt ggttcgatat cggctcggaa
    10081 atggtggata tgattggtgg cttgccttac cgtgacgaac tggattacgg caaaggcttg
    10141 gaagcaatgg atattttaat ggataaagcc aaatcgctcg gcaaaatgct ccacgtccac
    10201 gttgatcaat ttaactcacc taaagaaaaa gagaccgaac aactgtgtga taaatcgatt
    10261 gagcatggta tgcaagggcg agtggttgcc attcacggga tttccatcgg ctcgcacccg
    10321 aaagaatatc gaaaaatgct ttaccaaaaa atgcgtgata gccaaatgat ggtcattgcc
    10381 tgtccgatgg cgtggattga tagcggacgt aaggaagatt tacaaccgtt ccataacgct
    10441 ttaacccctg ctgatgaact cattcctgaa ggcattactg ttgctatcgg cacagataat
    10501 atctgtgatt atatggtacc gctttgcgag ggcgatatgt ggcaagaact cagcctttta
    10561 tcagcaggct gtcgttttac taacctagaa gaaatggcaa atattgccag tatcaatggt
    10621 cgaaaagtac tgggtttagt ctaactgaat aaacgaaagg cgaaccctag gttcgccttt
    10681 aataacagca taaccgatta tttaaacaag ttacccaaaa tcccttttgc cgcttgagct
    10741 aataagtcat ttgcatcaaa gttttttaag tcttcaacat taccattcgg ggttaaagta
    10801 tccacgattt ttggcaagaa ctgggctaat aaatcgctcg catcagcagc atcaacaccc
    10861 gcttcttgag ccacattttg aatgttttct tgcccgaaca cattactgat ttgttcgccc
    10921 gaaacaggtg cattgtcttc atcgctgctg atccaagtgc gtaacacatc atctaatccg
    10981 ccttgttgga atttggcaat cagcccttca atcccacctt gtgaggcgag taaattttga
    11041 attaattgag ttgcagttga ttggttattg ccactcaata ctgaagaagc caatgaacct
    11101 aaaatatttc ctaacataat ttctcctatg caagcggtac aattttgcga aaattttgca
    11161 aaaaaacagc aaacttcgac cgcttgtaat cactttaacg agctaaaatg gctcgcactc
    11221 tttccaaatc ttccggcgta tccaccccca cttgcggggc ttctttcgcc ggttcgatat
    11281 gaattttttc accgtgccat aaggctctta gttgctcaag ggactcaagc tgttctaatt
    11341 gtgtcggctg ccacgccaca tattgtttca caaaaccggc tcggtaagcg taaatgccga
    11401 tatggcgtaa ataaccttga gcggcaacaa aactgtcatc acaatccgga aaactgtctc
    11461 tggcaaacgg aatgctggct cgggaaaaat aaagtgccat tccgtttttg tctgccacca
    11521 ctttcaccgc atttgggtta aatagctctt ctcgggtcgc taatttcaca ccgagtgttg
    11581 ccatattgac ttgataagca tctaaatttt gagctacctg agtaacaatt accggtggaa
    11641 tcagcggttc atcaccctga atattcacaa taatttcgct gtcggcaatc tgcatttttt
    11701 cgatcacttc cgccaaacgc tcagtgccgg aattgtgttt ttccgaagtc atacagactt
    11761 ccgccccaaa agattgagcg agggcttcaa tttcagggtg atcggtggca ataatcacac
    11821 ggcttgcacc ggcaagctga gctttttccc aaacgtgttg aatcatcggt ttgcctgcaa
    11881 tatccaacaa cggcttacga ggcaaacggc tggaagcgta acgagcagga ataattacag
    11941 tgaatttcat aggctatcct tatgcttctt gagcatctaa gaaacgttct gcatccaggg
    12001 ctgccataca acctgtgccg gcagaggtaa tcgcttggcg atagttatga tccatcacat
    12061 caccggcagc aaatacgcct tccacagaag tggcagtagc attgccttcc aaacctgatt
    12121 tcactttaat gtagccgttt tcaagttcta attgaccttc aaacattgcc gtattcggtg
    12181 cgtgcccaat cgccacaaag aaaccatcaa ccttaatttc ttcgctcaat tcttctttgg
    12241 ttgattttaa acgtaagccg gtaacgccca tattatcgcc taacacctca tcaaccgtac
    12301 ggtctgtgtg cagaataatt ttgccttctt caatacgttt ttgcagacga ccaagcaaga
    12361 ttttttcagc acggaagcta tcacggcggt gaattaaatg cacttcactt gccaaatttg
    12421 ccaaataaag tgcttcttcc acagcagtat tcccaccacc gactaccgca accggtttat
    12481 tgcgatagaa gaaaccatca caagttgcac aagccgacac gcctttgcct ttgaaatgct
    12541 cttcagaagg caaacctaaa tatttcgccg acgcaccggt ggcaataatt aacgcatcac
    12601 aggtgaaagt gtataaatct ccgtttagcg tgaatgggcg tttagataaa tccactgaat
    12661 gaatatgatc aaacacgatt tcagtttcaa atttttcagc gtgcttgagc attttgttca
    12721 ttaactctgt gccggtaatt tgttcaaatt cccccggcca gttctcgatt tcatcggttg
    12781 tagtgagctg tccgccttgt tgcataccgg tgactaaaac cgggtttaga ttggcacggg
    12841 cggcataaac agcagcggta taaccggcag gccctgagcc taaaattaaa agtttggtgt
    12901 gtttagtggt cataatgaat cctctgaata aatattaatg tttattttag cctaaatttc
    12961 agttcaactc tattttcgtt ttcgctcaac ttttttcaat tttgtgacaa agttaacaga
    13021 aatccgactg tgtttcttgt aaattgtgac agtctaattc atttaaaagg ggcaaaaaga
    13081 tgaaaaaatt aattaattca atagaaaccg tcttagatga gcaactttct ggttttgtga
    13141 aagcacattc tgatttaaca cttaacacag aacctgtcta tatttaccgc acaacgccaa
    13201 gtaatcaacc taaagtagcc cttatttccg gtggtgggag tgggcacgag ccaatgcacg
    13261 ccggttttat cggtaaagga atgttagacg gagcttgccc cggagcaatc tttacatctc
    13321 caacgccgga tcaaatgttt gaatgcggtt tagcggttga tcgtggcgag ggtgtattgc
    13381 taattattaa aaactatacc ggtgatgtgc tgaattttga aacagccacc gagctacttg
    13441 cagatagcgg tgtaaaagtc gcaaccgtgt tggttgatga tgatgtggca gtcaaagaca
    13501 gtttatatac tgccggccgc cgcggtgttg caaatacagt attaattgaa aagctgttag
    13561 gagctgcagc ggataaaggc tataacttat ccgaattagc caaattaggg catcagttaa
    13621 acaataacgg gcattctatc ggaattgcct tatctgcttg tactgtgccg gctgccggta
    13681 agccatcatt tactttagct gaaaatgaga tggaatttgg ggtaggtatt cacggagaac
    13741 cgggcattga acgtcgcccc tttgaaaatc tggatagtac cgtgaaacaa atgtttgaga
    13801 ccttaattgc tcacggcgat tatcaacgta aaattcgcca ttggaataca caaacccaac
    13861 aatgggacga aatcgaagaa agcaaagtgg cattgaaaaa aggtgatcgt gttattgctt
    13921 tagttaataa cttaggagca gtgccactat ccgaacttta tgctgtttat gatgcgttag
    13981 aaaaagagtg ccaaactttc ggcttaagca ttgaacgcag tcttgttgga tcatattgca
    14041 cctcgctgga catgcaaggt atctctatca cattactgaa agttgatgag gatattctca
    14101 aactatggga tgctccggta aatacaccgg caatgcgttg gggtgaataa ttagattgaa
    14161 aggataagtt atgacaatat caaaacaaca gatcttaaaa tggcttgaaa attgccaaca
    14221 ggtaatgact gaaaaacggg attatttaac cgaattggat accgttattg gcgatggcga
    14281 tcacggttta aatatgcagc gtggatttag taaagcactt gaaaaagccc ataccgtgca
    14341 agataaagat atcggcaccc tgcttaaaac cgttggaatg acattattat cgcaagtagg
    14401 gggagcgagc ggccctctct atggttcatt ttttattaaa gcggctcaag gtgctacagg
    14461 caaagagcaa ctgagcttta acgagttact ggcacttcta cagcaaggag cagaagctat
    14521 cgtacaacgt ggacacgcac aagttggcga taaaacgatg tgtgatttat ggctgccatt
    14581 actcgaagac ttagctcaag ccgatagcac tcaaccaatt gctcaaatct tattacaagc
    14641 ggtcgaaaaa gcagaaaatt ttgcaaatca aaccgctcca ttaatggcga aaaaagggcg
    14701 tgccagttac ttgggcgaac gcagtatcgg gcataaagat ccgggagcaa cgtccagcta
    14761 ctatattttt aaagccttag ctgaagcatt acagtaggag gggtaaatgg ttaatttagt
    14821 gattgtttca catagtgcta aactggcgga aggcgtgggg gaaattttgg ctcaaatggc
    14881 acaggggggc tgtaaaatcg cccttgccgg cggcattgag gatacctcaa atcctatcgg
    14941 cactgatgcg gtaaaaataa tgcaagcgat tgaatcggta tttagcccag acggtgtgat
    15001 tgtgtttgtc gatttaggca gtgcaatttt aagtacccaa accgccatag atttactgga
    15061 gccggaactt gccgaaaaag tgatcattag ctacgctcct ttagttgaag gtgcttttgc
    15121 tgctgtagtt gcagcctctg ccggcgatag tttagaagcg gtattaaatg aagcgaacga
    15181 agccgctgct cttaagcagg aacaagccaa ttagtaaaaa tgccgtatcg aaaaatacgg
    15241 cattttagtt ttattagtac aatagtgaat aaagctgtct tctaaatttc ggcacaatcg
    15301 ggtctgtacc cagtgcagat aagattgcca gaaattggct ttttacctcg ccgttcgcta
    15361 catttaaatc cgattttaac caatcaaaaa gtaacgtgag tgcttcttca tttttatgag
    15421 cttggtgtag ctgatttgcc aatttaatcg caatttcagg ggttttgctg ttcgcataat
    15481 ccgcttgaag ctgttgtaat tcgggcgatt cgctggcttg ttccaataat tcgatttggg
    15541 cttgcaaacc gtgccaacgg ctgtctctgt cttgaatcgg gatcgcttta aggatttcac
    15601 gagcctcttc cacgcttttc attgcaatgt aagtttcggc atagagcaaa gcgaaatcgc
    15661 tgtttttctt atcggtcatt tcccacgctg atttcagcag cggcaaggct tcggtataac
    15721 gctgatcttg caataattcc aaggcttgtt gaaatttcag ctcttcctct ttcggtaaaa
    15781 tatttgccaa ccgaatccgt aattcctgct cacttactgc accttgaatt gcatcaatcg
    15841 gctgaccgcc ggcaaacaaa tagagcgttg gaatttgttg aatgcgaaat tgagcagcca
    15901 atgcttgttg ctcatcacaa tttaccgtcg ccaataaaaa ttggttaggg ttttcatccg
    15961 aaatacgacg tagtaaggca tccatttcca atgaagccac ttcccttggc gaaataaaat
    16021 gaaacaccgt cggcacttgt gccgctgctg cccatacttc actaaaattc tgctcgttta
    16081 catttacaaa gtagtccata caaaaccttc ttaagctaaa aattactttt tatattgtag
    16141 cattaataag aagtttcaat aggtagcccg tttctctccc acgcttcaaa accacctttt
    16201 acactataaa cattctcaaa cccttgttcg attaaaaact gtgctgtact ttggctactc
    16261 actccgtgat agcaaatcac aaccaccggt tcgtcataat cgacctcgtc taaaaactta
    16321 ccataactct ggttggttaa atgaaacgca tcctgtgggt ggctgtaaac ataacggcgg
    16381 gcatcacgca catcggcaag caccgcacct tcgttttcga ttaattccca agcctgttgc
    16441 gggctaattt ctttaaatgt agtttccata atcactttcc ctcaaaaaac gtgcaactat
    16501 accataaaat tctaaaaaga atggctatta ttccaagttg aaaatttgat gtttgtcaga
    16561 tataattgca aactattttc aaatgcactg atttaaaact accacagttt gaggatttta
    16621 tggagcaatt atttcaattt ttagaacatc atactgactt tgttatatta ggattactag
    16681 gcttattaag cgtaatctgc ctttggaaag ctattgaacg tattatgttt tattccaaag
    16741 tagatatttc aaaatacgat accctcgaag aattagacat cgatttaacc cacaatttaa
    16801 ctaccatttc gactatcggc tctaatgccc cttatatcgg cttattagga accgttgtcg
    16861 gtattctttt aactttctat catttaggtc aatcaggtgg tgatattgat gcggcctcta
    16921 ttatggtaaa cctttcttta gctctaaaag ccactgctgt cggtatctta gtggcaatta
    16981 ttgcaatggt tttctacaat ggttttggtc gtaaagtgga agaaaacaaa ctgaaatggt
    17041 tggcattaca atcaaaaaag cctcatcaag cggtgtgatt tagtccattt tttgcaaatt
    17101 aataaggcag aacaatgaaa aaattcgatg aaatcaacat cattccattt attgatatta
    17161 tgttggtatt actggctatc gtgttagtta ccgcctcttt catttctcag ggtaaaatcc
    17221 aagtaaatgt accgaaagca agtacaacac agccaatgaa agcagacgat ttagcgaagc
    17281 ttttaaccat taccgaaaat aatgagtttt tctttaatga tcaagcaata actaaagagc
    17341 aattaattgc tgaagttgcc acttgggata aaagccaaaa agtgtcactc aaagttgatg
    17401 gtgcagtcgc cttcgaaaaa tttgtggaat taaccgatat cctttctgca aatgaaatta
    17461 aaaatgtagc aatcattact aaaaaagaaa ctgctccagc accttcaagc acacctggta
    17521 gccctgcaca agtgccagca gtcgcacctt aaatagggta aacaggtatg aaaaaaaaac
    17581 attctcgtat cggcttattg gcatctttgg ctattcacac tgccttaatt ggtggcactt
    17641 ttgcttggtt acataaccac aaagtggaaa aaattgccga gcaaaactct ctttctatgg
    17701 aaatggtggc agcattatta gagcaacctc aggttgccgt tgctccggat cctattaccg
    17761 aagaggtcaa agaaatcgaa ccggagaagg cagaagtacc acctgaacct aagcttactc
    17821 cggagccaac tccagagccg gaggcaattc cagatcctac gctaaatccg aaaaaggaaa
    17881 aaccgaaaga agaaaagcgt aaagagcgac accaccataa acataagcat cgtcacaaag
    17941 agaaaaggga agagctacaa gagcaagaaa aaccgaaaga taagcctaaa gagcgcccga
    18001 aacatcatca caagcatatt aaagcattag agcgcgggcc agagataaaa caaggtattg
    18061 tcgctaaagc gattccaaat gcggctgaag gtgaaaaagt ggttgcgggg gttgttggag
    18121 ggcaaaaaaa cggttcacca aattcaacct caactacagg ttcagcaaat actggggcta
    18181 gcagtggtaa tgccagtgaa cttgctgctt ataaagtcgc attacagcga gcattacagc
    18241 gtaaggcgaa taactcttac cctcagcgtg aaaaaatgat gcggaaaacc ggtacagtta
    18301 cgattaaatt taccattaca tcgtccggtt caattacaaa tgtatccgta ctcaattcat
    18361 cgggaaatag taatttagat aacgctgcgg tacgcagtgc agaaggtttg agtatgcctc
    18421 cgccaccggc aggcttccct cctacggtaa cagtaccggt aagatttgcc cttgaataaa
    18481 aacagccctc aaaagcaatg tacttttgag ggctttttat tgcaaaaaat tgactaaaaa
    18541 tgaccgctta ctgcttgcga atatcgccat ttctcacatt cggactcact tcacccgtac
    18601 gagtttctgt gcttaacgag gtagaattta ctttcgtaaa agtataatcc gctttgctga
    18661 attcacctgt ttgattattt aagaagaaat tgcggtactc ttttacccat aatgccgttg
    18721 cgccacaaac tgccacaggc ataactacta aattcacaaa tggaatcatc gtgaataggc
    18781 tcactaaagc accaaaggta aaatttaaag tgcgagactg cgacaacgca tttcgcatac
    18841 gctgaaaact gattttatgg ttatcaaacg gatagtcaca atattgaatg gcgatcaacc
    18901 aagcaccgaa gataaaagtc agcacaggtg ctacggtttg cccaaccaca ggcaaaaagc
    18961 ctaatacaaa aagagcaatc agccttggaa tgctatacat cattttttgc cattctcgtt
    19021 tcagcattcg aggcacatct tttaacattg caccgaatga catatcgccc aaaggctcgc
    19081 cggtaagttg cagttcgact ttttctgcta ataacgcatt aaagggagca gcaacaaagt
    19141 tagccaaggt agtaaaagca aaataaaatc ccactaatat catcaaaaaa atcagtggaa
    19201 tcagtacaaa gctaagccat tccagccagc ttggcagtaa catatccaac aagccaccga
    19261 tattggcaaa aaatccccaa atcagcccta ccatcagcac ggtattaata agaattggga
    19321 aaatcacaaa aggcaaaaat tgacgttgta acaataaccg ccagccatat ataaaataat
    19381 gaaagcctaa tccgacttga ttttgcggct cagaaaaaac tgaagacata atgattcctt
    19441 attttctatc aaaattaatt agaaatatgg taagctacgc acattatttt cgcaaatatg
    19501 aaaatacatt ttttattgat tatttgataa agaggacaca atggaactgc atattatttt
    19561 ttttattctt gccgggctac tgattgctgt actaattgga tttagccttt ggtccgctcg
    19621 tcgtgaaaaa tcaaatattt tttcaaatac attctcgacc agacctattg ctgtgccacc
    19681 acagaataat aaaatgagca atagcatccc aacctcatta cagccacaaa atgaggatta
    19741 tcaagcaatg tctgtgccgg aagaaaatcc gcaacagata caacaggaag ttgaaagctc
    19801 gctgcaaaat atcaaaatca gtttgccggg cggaaatcct cagcaacagc ctgagcaaat
    19861 tcagccgata caatcggtag cagaaccaac ggttagcact tacggtcaga aaatttctaa
    19921 tcagcaacat atccaataca atacgccgga ttttgcaaac ccaatggtga acacagtggt
    19981 ggaagaaaca cctcaagcag tagctatcga acctcaagaa acccaatcgg cagaggaaga
    20041 aaatccattt gtaaccctct atctttacgc accacaaaat gaagctttcc gcggtgattt
    20101 tgtagtacat tatttagaag aagttggctt acgttttggc gaacaccaaa ttttccaccg
    20161 ccatcaacat attgatgatt cattaagtcc gattattttc agtgcagcaa atttaatgaa
    20221 cccaggaacc tttgatttaa ataatatctc aaactttgcg acagctggtt tagtgctatt
    20281 tatgcaactt ccaaccgaag gcaatgatgt ggctaatatg aagttgttaa tcagtaacgc
    20341 tgaacactta gcagcatcgt tagggggatt cttatatgac gataatcaac agccttttgg
    20401 cgaagaatcc cgccaacgtt atatgcaacg tgtgatgcac taattgcgaa aaaattaata
    20461 aatccgaccg cttgtacaag cggtcttttt tgtagagaat tttaccaatg tctttattta
    20521 accaaacctc tttatttgaa gagaatgcca cgcccgagtt agtcgagaaa atcgaaacta
    20581 ttcgccaaac attgcgtcaa tacgagtatg aatatcacgt tttagataac ccaagcgtac
    20641 ccgatgccga atacgaccgt ttaatgaacg agctgaaaaa tctggaatgg cagcacccgg
    20701 aactgattag tgccgactcg ccgactcaac gggttggtgc caaaccgctg gcaggctttg
    20761 ctcaagtcac ccacgaaatc cctatgcttt cgttggataa tgccttctcc gatgaagact
    20821 tagatggctt tttacgccgt atggaaagct atatcaccgc ctctccttcg caactggaat
    20881 tttgttgcga acctaaatta gacggcttgg cagtgagtat tttatacgaa aacggtatac
    20941 ttacccgagc tgcaacccgt ggcgatggca caaccggcga agaaattacc gctaatatcc
    21001 gcaccattcg caatattccg ttaaagctca acacgactaa tccacctgct cgattagagg
    21061 tgcgaggcga agtctttatg ccacaatcag gctttaatga gctgaatgag aaagccttag
    21121 ctaaaggcga aaaaaccttt gccaaccctc gcaatgccgc agcaggttcg cttcgccagt
    21181 tagatccgaa aatcacccgc cagcgtccgt tagtgctgaa tgcctacgga attggggtgt
    21241 atgaaagcga tgacgaactg ccagccaccc atttcgaacg tttgcaatgg ctgaaatcgc
    21301 ttggtattcc tgtaaataat gaaatccgct tggttcaagg caaagctgct ctcttggctt
    21361 attatgcgga gattcaggct aaacgcccaa cattaggcta tgatattgat ggcacagtat
    21421 tgaaagtaaa tgacattgcc ctacaagagc aactcgggtt tatctctcgc tcctctcgtt
    21481 gggcgattgc gtataaattt ccggctcaag aagaaatgac aatcctcaaa gaagtggact
    21541 tccaagtggg aagaacaggg gcaatcaccc ctgtggcgaa attagagcct gtttttgtcg
    21601 ctggcgtaac ggtaagtaac gcaacgctac acaacggtga tgaaatcgag cgacttggta
    21661 ttgtgattgg cgatacggta attattcgcc gagcgggcga tgtaattccg caaattacag
    21721 gcgtgatgtt agaacgccgc ccggaaaatg caaaaaaaat tgaatttccg accgcttgtc
    21781 ctatctgtca ctccgctgtt actcgggtag aaggcgaggc agtagcacgc tgtaccggtg
    21841 gtttattctg cgaagctcaa cgtaaagaag ccttgaaaca tttcgtttca cgcaaggcaa
    21901 tggatattga cggcgtaggc gaaaaactaa tcgagcagtt aatggctcga gagctgattg
    21961 aaacgcctgc cgatctgttt aagctcgatc acactaccct aatgcgatta gagcgaatgg
    22021 gggaaaaatc ggctcaaaat gctctcaaca gcatcgaaaa agcaaaaaac actactcttc
    22081 ctcgcttttt attctcgtta ggtattcgtg aagtgggcga ggcaaccgct ctcaacttag
    22141 ccaaccattt tggtagctta gaggcgatcc gccaagccac ttttgagcaa ttaaaagaag
    22201 tgcaagatgt gggcgaggtt gttgccaacc gtattgtcag cttctggcaa gaaccgcata
    22261 atgtggaagt ggtggaagat ttgatcgctc aaggtgtacg ttgggaaaac gttgtgaaag
    22321 ccgaaattgt agataacccg ttaaaagata aaaacgtggt cttaaccggc acgctaaccc
    22381 agctcacccg tgatgaagcc aaagccctat tacaaagttt gggctgtaaa gtctccggct
    22441 cggtttccag caaaaccgat tacttaatcg ccggcgaaaa agccggttcc aagctggcaa
    22501 aagctcaaga gttgggaatt cagattttaa gcgagcaaca atttattgag cttactcaac
    22561 attaacaaac aaaggggcag atgattgaaa tctgcccctt attctatcta ttgaatgctt
    22621 aaaagcatta accaatcaat ttaaactgct gagctaaaaa cagtaacgta aagctagtta
    22681 agtaaattaa gccgataact gccaaccaaa ataaccagcc ttttgcacga tgctcacctt
    22741 ttagcactaa agagaaattt aaccacgcaa aaacaggggt agaaacaaat gatgaaatca
    22801 ttgcaaattg taacattttc gccacatcac tcataaattg gctaataatt aaaatgccga
    22861 tgagtgcagt taccgtcatc caaatactta attctgtttg tgaatgttgc ttacgcccgc
    22921 taattaaacg caacgcttct gcattcgctc gtgaatatcc atcaataaca gtgatcgttg
    22981 tgccgaacat acacataaat gcaataaatg cgattaaaag cttagaccag tcaccaatcg
    23041 caaaagcata cattttgatt aattgagcaa tataggcaac accggcttgt tctactttct
    23101 cacctgaacc gtactgaact aacgcaccta atgctaaaaa aacaatcgcc aaaattgccg
    23161 tgccgatata acccacatta aaatcaaaaa taccgtcctc ataattcact ttacttaaac
    23221 gttttttcgc aataacccac attgagttaa tggcagaaat ttcaattggt gcaggcatcc
    23281 agcccattaa tgcaacgatg aacacaagtg ctgctaaatt ccacggcgaa ggctctacga
    23341 aatctggagc aatttcaacg ccacctttta aagcagcaat gatcacggca gataccgtag
    23401 agatggttaa agcaatcata atccatttag ataatgtatc taagaattta tattgcccta
    23461 gaagtaaaat accccaagtg accacaatca caatagttcc tgccattgga atgcttaaat
    23521 ctaacggaat acctaaagca gaaaaaataa attttagaat cgctgctgaa acaatcgcca
    23581 ccgcagctgt gttaatcatt gcagcaaaaa cgttaaggat aaagaaaata ataagataaa
    23641 actttccttt ttctttgtag ccttccaata aagttttacc gctagctaag gtatattgca
    23701 caccaaagcg gaaaaacgga tatttaaata aattggctaa cagaataata atcgccagct
    23761 gccagccata aatcgcacca gattgtgtag aggcaataat atgcgaacca cccactgccg
    23821 cagatgccat caaaatcccc ggccccattg cagcaaattt actttgccaa gttgaatgtt
    23881 ctatttgttg tgtcatatag ctttccttat cacattacta tcaaaacaag atggtttcat
    23941 tctacttata aaaaccaaat aaaaacactc ctctagaaaa gatgttcat
//
LOCUS       DANLXS010000015        22424 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.015, whole genome shotgun sequence.
ACCESSION   DANLXS010000015 DANLXS010000000
VERSION     DANLXS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22424
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.015"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..654
                     /locus_tag="PXD71_000489"
     CDS             <1..654
                     /locus_tag="PXD71_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6290646.1"
                     /translation="WFTASEVAGMGNLPEQPSNVTRRATKNNWLKRQIEGKRGVAFEY
                     HYSSLPISVQQELGFAQTAVKEEPRTLVAAQTADEMERVPFYNVQASAGFGAFNSEVY
                     APDDYIGLSKRWLDVRGFYLSYLAFITASGDSMYPTISDGDMLLVNRATTQPKDGKIY
                     VFRQGEQLWVKRVQGIMGGIRLISDNKELYSPIDVIFDETLDFEVIGQVVYIGHEMI"
     gene            702..3554
                     /locus_tag="PXD71_000490"
     CDS             702..3554
                     /locus_tag="PXD71_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6290647.1"
                     /translation="MVQAVSFSHFFAIHYFYFFLHKTKGQCTAKPLFSILLFHLFPKN
                     SAKFLLFFYFLVLMVLYSNEVANGKLLITGGTNLNGTLSAEGTSEIILSGRPTPHAYD
                     KVANKEVLIEGDWINRSFNATIFAAKDNGKLEISRNVANINGNFNLTDNATAQIGFTQ
                     GTSQACIRSDRTGVATCNINATLSETDLNSWERTKVAGNVSLADNSTFSLGSKADLTG
                     SITAQESTKVQLNDGSIANLTGLSTTGIFNGANGSTVNLSGVWTTLTNTVVDVLNVAN
                     GSQIHLNTPTATKQNTLTANTLTGNGIFKFVADLTKTASDIVTIKDTASGQFSIEIST
                     NGSAHSAQKIKLFETTNEEDFSLNLANEVRSGEYRYHLLKEGNVYYLNPEKEEEPKIT
                     QKGPSETADPLPEYTGDIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNG
                     NAHLIASELTAQTNAVLGVTTVLDHVIHSEGTQKQNTLTANTLTGNGIFKFVADLTKT
                     ASDIVTIKDTASGQFSIEISTNGSAHSAQKIKLFETTNEEDFSLNLANEVHSGEYRYH
                     LLKEGNVYYLNPEKEEEPKITQKGPSETADPLPEYTGDIEYSEKGPGETADPLPEYTG
                     DIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNGNAHLIASELTAQTNAV
                     LGVTTALDHVIHSEGTQNWKVWSKTDYQTTEHRSAGYGFNQDANVIHLGLEKALDNQV
                     TLGMILSQSKARNNFDHYYSGKGRLTMLSMYAKKTWQNGVFVAIDTGFGKASNRLTYQ
                     ANTVKLDRSVFVTGLSIGKAWESANVNIIPSFSARYHHLSSAGNQLVDAKIETNAVDL
                     LALQAGLSINKTLELNGLQIKPEIGSYFVDASHGKLRTRFNNLQIEQQMGRYFKQEAG
                     ISANYRNINAGIQAGFLTGNTLNKQRYISFKVTYEW"
     gene            complement(3629..4465)
                     /locus_tag="PXD71_000491"
     CDS             complement(3629..4465)
                     /locus_tag="PXD71_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL6290648.1"
                     /translation="MLPHIYIGTGGYADTDLIGTLYPFGTAKEDFLFVYSQHYDTLEI
                     NSTFHAPIGQKAFEGMVQKAEGRLNFSVKLHQDFSHQRTATLDQAQLFLNALAPFTAE
                     GLLANLFVQFPTQFERMPLNRRYLAELCQWFADYPLAIEFRHPSWHIPAVFDTFAKSS
                     NLIWCNVDYPQNIGLPAFHFFANQRTAYLRLHGRNPNWWKGQSAAERHDYRYSDEELK
                     GLAELLFSRRTEFDRLYLYFENTTKSHAFYNIQTLKGFLAEKGFQIKPVPENLLGQQS
                     LF"
     gene            4573..5448
                     /locus_tag="PXD71_000492"
     CDS             4573..5448
                     /locus_tag="PXD71_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6290649.1"
                     /translation="MWIWFTLLAAVAQAGRNAFQKQLSVNVPVLGVTLARFFYAVPLA
                     SGYLYFLYQYDDNLNIPHFPPLFFVYAIGAGLAQILATSLMVQLFHLKNYAIGVGLAK
                     SEAVWAALLGVLFFGVTISGIGWLGVVLGGVAIFLMTGSGNLRQLSWKTLFLGIASGL
                     CFALTSLWVREASLQLNSHYLLSAAWVLFSVISFEAVILVIYLALLQKETLIKFFRFP
                     KLGMLTSLFSFLGSFGWFNAMSLNHVAFVKTLGQVEIFFILGISYFVFKERLKIQDFI
                     GLILVVIAAILVVLG"
     gene            5584..6024
                     /gene="nudB"
                     /locus_tag="PXD71_000493"
     CDS             5584..6024
                     /gene="nudB"
                     /locus_tag="PXD71_000493"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDL6290650.1"
                     /translation="MNYKNPHSILVVIYAKNTQRVLMLQRKDDPDFWQSVTGSINIGE
                     TPLEAAEREVSEETGLQTFEQKQPLVDCQKQIEFEIFPHFRYKYAPNITHCVEHWFLL
                     PLDSEQEPILTEHLAYRWVSVEDAVRLTKSPNNAQAIQEYLTSK"
     gene            6037..6777
                     /locus_tag="PXD71_000494"
     CDS             6037..6777
                     /locus_tag="PXD71_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6290651.1"
                     /translation="MAGHSKWANIKHRKAAQDAQRGKIFTKLIRELVTAAKLGGGDTS
                     SNPRLRAAVDKALSSNMTRDTINRAIERGVGGGDDTNMETKIYEGYGPGGTAVMVECL
                     SDNANRTISQVRPSFTKCGGNLGTEGSVGYLFSKKGLILVANADEDALTEAAIEAGAD
                     DIQPQEDGSFEIYTAWEDLGSVKDGIEAAGFKIENAEVTMIPSTTVELDAETAPKLLD
                     LINRLEDCDDVQNVYHNGEISDEVAALL"
     gene            6907..7434
                     /locus_tag="PXD71_000495"
     CDS             6907..7434
                     /locus_tag="PXD71_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290652.1"
                     /translation="MKLVKFVPALAATLVLAACSNANTQSAPQQAQDAVTKVAEQATQ
                     QVNEIAGNAPRASGAVKVEKATDGAKSIVYRCQSGKRVTANYVFQGEEAKAVNLLVQS
                     GGKTQKIPTLTRDASNQDSTSFKTDKYSWDLDTGFTLSSNGQSGMLTKYGAKTDTIVA
                     KLCDVDKALTSKLNK"
     gene            7501..8079
                     /gene="ruvC"
                     /locus_tag="PXD71_000496"
     CDS             7501..8079
                     /gene="ruvC"
                     /locus_tag="PXD71_000496"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDL6290653.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQNGRQLEYLGSGAIRTTAEDLPTR
                     LKRIYAGVSEIITQFQPNMFAIEQVFMAKNPDSALKLGQARGTAIVAAVNHDLPVFEY
                     AARLVKQTVTGIGSADKVQVQDMVTRMLKLSAKPQADAADALAIAITHAHSIQHSLIV
                     AKQSQQNVSSEKEQILALMKTRYSRGRFRLKG"
     gene            complement(8145..9425)
                     /gene="murA"
                     /locus_tag="PXD71_000497"
     CDS             complement(8145..9425)
                     /gene="murA"
                     /locus_tag="PXD71_000497"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL6290654.1"
                     /translation="MEKFRVYGPFSLNGTVDISGAKNAALPILFAAILAEKTVTLKNV
                     PDLKDVDTTFKILRQLGVTVEKGSDKGVVHIDASKINNYVAPYELVKTMRASIWALAP
                     LVARFQLGQVSLPGGCTIGARPVDMHISGLEKMGAKIELDEGYVKATSNGRLNGARIY
                     MDKVSVGATLSVMMAGTLAKGTTVIENAAREPEIVDTADFLNAMGAKISGAGTDMITI
                     EGVEHLGGCEHSVVPDRIETGTFLVAAAISGGRITCRNTKADTLDAVIEKLREAGMQV
                     DVTENTITLDSLGRRPKAVNIRTMPHPGFPTDMQAQFTLLNTVAEGTSRITETIFENR
                     FMHIPELIRMGAKAEIEGNIAICYGVEQLKSAEVMATDLRASISLVLAGCIATGETIV
                     DRIYHIDRGYEHIEEKLRQIGARIERFSAPFEGE"
     gene            complement(9535..9795)
                     /locus_tag="PXD71_000498"
     CDS             complement(9535..9795)
                     /locus_tag="PXD71_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL6290655.1"
                     /translation="MNPSQIEEILKQALSDATEVHAQGENAHFGVIVVSDSIASLSKV
                     KQQQAVYAPLAEYFTTNAIHALTIKVFSVEKWKTERLLNMVG"
     gene            complement(9828..10151)
                     /locus_tag="PXD71_000499"
     CDS             complement(9828..10151)
                     /locus_tag="PXD71_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDL6290656.1"
                     /translation="MPQKTLQWDIQQNNESLFVKLSGELTRNTLLPLWKQRASFLSPT
                     GKQSLYWDLKEITRIDSAGFTLLAELLNHYHKIIPNSLINTPDSVRTLAALYDLDGWF
                     EQFII"
     gene            complement(10163..10798)
                     /gene="mlaC"
                     /locus_tag="PXD71_000500"
     CDS             complement(10163..10798)
                     /gene="mlaC"
                     /locus_tag="PXD71_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDL6290657.1"
                     /translation="MLKNIKKIIVTGLVAVSALFSTTAFAETSPYVLMQQTADKLFGD
                     IKANQAKIKSNPEYLRTIVRNDLMPHVHVKYAGQLVLGKNLSAATDAQKEAFFNAFGQ
                     FVEQSYAQVLTQYQDQSVQIESPKSVDGKSIVSIRVNVSGKGAAQPIKLDFKWRKNSK
                     TGEWQAYDMAAEGVSMVATKQNEWAGVIRQKGIDALIAQVAQSAKQPITLK"
     gene            complement(10844..11368)
                     /gene="mlaD"
                     /locus_tag="PXD71_000501"
     CDS             complement(10844..11368)
                     /gene="mlaD"
                     /locus_tag="PXD71_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDL6290658.1"
                     /translation="MRQSIKYEFWVGLFVLLGLAALVFLGLRVANVQGFSNEKTYTLY
                     ATFDNIGGLKVRAPIKVGGVVVGRVADISLDNKTYTPKVALAVNESFNQIPDTSSLSI
                     KTSGLLGEQYVALNVGFVIEGETAMMKEGDTFVDTNSAMVLEDLIGQFLYGDKKSDKT
                     EDEKASDSAEPKAE"
     gene            complement(11389..12165)
                     /gene="mlaE"
                     /locus_tag="PXD71_000502"
     CDS             complement(11389..12165)
                     /gene="mlaE"
                     /locus_tag="PXD71_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDL6290659.1"
                     /translation="MVNFISSIGAVVINFIRTLGRSAFMLWGALIGKPEFRKHTPLLI
                     KQLYVLGVQSLLIIMLSGLFIGMVLGLQGYVVLVDFAAESSLGTLVSLSLLRELGPVV
                     TALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLRRIIAPRFWAGVISMPVLAAI
                     FTAIGIWGGSLVGVDWKGVDGGSFWSVMQNSVNTSDLINGFIKSVIFAFAVVWIALFN
                     GYDCVPTSEGISQATTRTVVNASLVILGLDFILTAIMFGG"
     gene            complement(12158..12961)
                     /gene="mlaF"
                     /locus_tag="PXD71_000503"
     CDS             complement(12158..12961)
                     /gene="mlaF"
                     /locus_tag="PXD71_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDL6290660.1"
                     /translation="MTKTENLVEVKNLTFKRGERVIYDNLNLQVQKGKVTAIMGPSGI
                     GKTTLLRLIGGQILPESGEILFDGRDICKANNKELYEIRQRMGMLFQSGALFTDISTF
                     DNVAFPIREHTRLPEELIRKLVLLKLEAVGLRGAANLMPSELSGGMARRAALARAIAL
                     DPDLIMYDEPFTGQDPISMGVIVELIKELNQALNLTSIVVSHDVKEVLSIADYAYIVA
                     DKRVIAEGTPEILQASEDPQVVQFLAGKADGPVRFHYPAKDYTEELFNG"
     gene            13186..14580
                     /locus_tag="PXD71_000504"
     CDS             13186..14580
                     /locus_tag="PXD71_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HDL6290661.1"
                     /translation="MNKTMKKSFLTAFILGSTIAAMPAYAEATLPTAVNGQAVPSLAP
                     MLEKVRPAVVSIAVEGKTKGDSRRSREIPEEFEFFFGPHADLFGNRSSAPRNFRGVGS
                     GAVINAEKGYVITNNHVIDNADKITVKLEDGREFKAKLVGADPLSDVALIQVEDPKNL
                     TEIKFADSDKLQVGDFTVAIGNPFGLGQTVTSGIISALGRSTGQSDEGYENYIQTDAA
                     VNQGNSGGPLINLNGELIGINTAIISPSGGNAGIAFAIPSNMANSLVTQIIEFGEVKR
                     GMLGIKGGELNADLAKEFNISTQQGAFVSEVLPDSAAAKAGFKAGDVITEINGQKIRS
                     FSELRAKVATSGVGKEIELTYLRDGKEAKAKVTLQSDSEAKVTASNLIPALKGADFNN
                     YNAKGIKGVEISNVEKGSIAEMRSLKKGDIIIGVNRHSVENIADLRKILETKPSVVAL
                     NIVRDGANFYVILQ"
     gene            complement(14632..15393)
                     /locus_tag="PXD71_000505"
     CDS             complement(14632..15393)
                     /locus_tag="PXD71_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDL6290662.1"
                     /translation="MKTREQLSQENYQQAVKFAQHFADDVRQEFEREAEQRYQFERTQ
                     FEQSVLQNNAQNRPLAKIASLSCVAILLLAMTYYGLSGRYQVVQSGLQQHQDFQQQMS
                     DAHGTSKNERYILSLQNQLRENPNNGDLWYELGQAYALSNDFDSALVCYDNAEKLLGK
                     RAAILGAIATARYYDNGQKMTVEIQAIIEQALQLDKNENASLLLLASDSFLNNNFQQA
                     LDNWRKVLDSNNDSINRRAIIQSMEMARQMLNSQQ"
     gene            complement(15380..15832)
                     /gene="nrfF"
                     /locus_tag="PXD71_000506"
     CDS             complement(15380..15832)
                     /gene="nrfF"
                     /locus_tag="PXD71_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDL6290663.1"
                     /translation="MRNSIISIGFLQKIRKILPLVFGLLLFVGTVQAEMVDTFQFQNE
                     ADRVRAISLARSLRCLQCQNQNLVESNATAAYNMRIEVYEMVNQGKSNEEIIRIMTER
                     FGSFVNYDPPFTPQTWVLWGLPVGLFSLLFLAILWRTRKNKGNKNENA"
     gene            complement(15822..16355)
                     /locus_tag="PXD71_000507"
     CDS             complement(15822..16355)
                     /locus_tag="PXD71_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL6290664.1"
                     /translation="MKKILLLIPLLLLVVIVGFLTVPLMNKDALAPTEDWQGKPFPEF
                     VGKNLLDSNAHLNNNSLPKEPYILNVWVSWCTWCIKEFPILLELKQKGVPIVGLTYSD
                     RPNDALNALKNWGNPFSLIIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
                     DFAQDFLPKLEALRNEK"
     gene            complement(16352..18256)
                     /gene="nrfE"
                     /locus_tag="PXD71_000508"
     CDS             complement(16352..18256)
                     /gene="nrfE"
                     /locus_tag="PXD71_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HDL6290665.1"
                     /translation="MLPELGYFSLLLAAISAIFQVGFALLGEVRKQYHWLALAPLFTY
                     LQAAFTTFSFSTLAYAFLTDDFSVIYVAAHSNSQLPDFFKFAATWGGHEGSMLFWLTA
                     LVIWSAVFCKFSQKIDRLFANRTLAMLGLIALGFLLFILLVSSPFERGFPPPPEGRDL
                     NPMLQDIGLIFHPPLLYLGYVGFAVSFAMIVAALVSGGMDAAIMRWLRPWTMISWGFL
                     TAGIILGAWWAYYELGWGGWWFWDPVENASLMPWLLGTALVHSLIVSQQRGIFYYWTI
                     LLAIFAFALSLLGTFIVRSGVLTSVHAFAVDPDRGMALLILFFGLSFIALALFAFRAN
                     LWQGKVRFNLLSKETAFLLINGLFSVAASVVLLGTFYPMIFTVMNWGSISVGAPYFNS
                     VFTPLALLLMIVMGFAVVLGWKQISNKQILVKLWLLPIAVGLALGLIYASISQREHFA
                     LQPLPTIFISLAIWIILTHLPYSQKMWQIRPLAMRLAHIGFAVCVIGAMMNSYYGDEI
                     GVRLKPNQAAELAGYTFKYEDYHDLIGPNYTAEQAVFSISRDEQLLATVTPERRYYDV
                     RTMTMAEVGLFHHNLDDIYIVMGDKFGNLEYAFRLHYKPYVQALWLGGMMMVMAALLA
                     LFGYRRENKK"
     gene            complement(18526..19473)
                     /gene="nrfD"
                     /locus_tag="PXD71_000509"
     CDS             complement(18526..19473)
                     /gene="nrfD"
                     /locus_tag="PXD71_000509"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDL6290666.1"
                     /translation="MNEYVPFQTPNLVWDGTIAIYLFLLGISSGAVQLAIAYRNSGVK
                     MEKPSQNWIIRSAAILGTLPTIIGLLLLIFHLTKPWTFWKLMFNYNFTSVMSMGVMLF
                     QLYMAVLVVWIAIMFKDWIVRYLPVVSGLIDFAEAKLLKPIEFILFVLAAVLGAYTGF
                     LLSALVSYPMLNNPVLPALFLASGASSGIAATFLVILLAGKLSGESHEVHFMHKFEVP
                     IMVTELGLIVAFFVGLHFGGADKSLALTHALNGFWGNVFWIGVMLIGIAIPLVANIFG
                     SHRLKHNVKFIILISVFDLIGVLCLRYFILYAGQLTVAS"
     gene            complement(19473..20150)
                     /gene="nrfC"
                     /locus_tag="PXD71_000510"
     CDS             complement(19473..20150)
                     /gene="nrfC"
                     /locus_tag="PXD71_000510"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDL6290667.1"
                     /translation="MTCTRRDFVSGAGAIAVVASTGLATSITLATEKEQKKIRYAMVH
                     DETACIGCTACMDACRTTNKVPEGVSRLEIIRSEPFGEFPNVQYEFFRQSCQHCTNAP
                     CVSVCPTGASFIDPETGIIDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
                     TNLAEGKQPACVEACPTKALTFGDMNDPSSDIFHKVKNNPVYRTKTALGTEPNLYHIP
                     FGKGEHR"
     gene            complement(20150..20806)
                     /gene="nrfB"
                     /locus_tag="PXD71_000511"
     CDS             complement(20150..20806)
                     /gene="nrfB"
                     /locus_tag="PXD71_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDL6290668.1"
                     /translation="MKKLISKAGRAVALIQAVVFAGFFAISSANALMPEQPKPLWQSE
                     TADDAKVAQDARRDPNKYCTQCHESANATGKPFHHAGKHFQKDVKSPNNGEPLTCISC
                     HGNISESHRKGAKDVMRFNPHGKASNPSLERSVNEQNQVCFACHTAEKLREKFWAHDT
                     HATKIACTNCHEIHPEKDPMKDIPEKDRIKLCTDCHTKIHSGEFKNPEMKATEAKGTK
                     "
     gene            complement(20825..22327)
                     /gene="nrfA"
                     /locus_tag="PXD71_000512"
     CDS             complement(20825..22327)
                     /gene="nrfA"
                     /locus_tag="PXD71_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDL6290669.1"
                     /translation="MKFLIKSLAVATISILGCLQTALAEEAKTESLTDKAVKHEKLGV
                     KIESANHLFAEKYPLQYDSWKSTAKSTDRGSALEADPRYVILWAGYAFAKDYNKPRGH
                     FYAVTDVRDILRTGAPKDENDGPQPMACWTCKGPDVPRLIEEKGERGYFDPKWAKYGA
                     EIVNSIGCADCHDTTSEEFKQGKPALRVARPHVLRALNTVGWKFEDLDKHGKRPAVCA
                     NCHVEYYFKNKTDVTFPWDKGVDVDSIEKYYDEINFTDWTHALSKAPMLKTQHPDFEV
                     WSQGTHGKNGVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFEATCKTCHEQSKETLEK
                     RVKEYKHEVKEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKEILMAIRHAQWRWDYSA
                     AGHGNHFHAPDVMLRTIATGIDRAADARAKLGVVLAKHGVTTPVAIPDISTKEKAQLA
                     IGLDIPKEQAAKDEFLKTVVPQWEKEARAKGLLPAEEADKPVAAPKAEAK"
BASE COUNT         6747 a         4850 c         4528 g         6299 t
ORIGIN      
        1 tggtttacgg catctgaagt agctggaatg ggaaatttac ctgaacaacc atcaaatgta
       61 acaagaagag ctaccaaaaa taattggctc aaaagacaaa tagagggaaa aaggggggtt
      121 gcttttgaat accactacag ctctctccca ataagcgtgc agcaagagtt aggctttgct
      181 caaacggcag taaaagaaga gcctagaaca ttagtggcag cccaaactgc agatgaaatg
      241 gaacgtgtgc cgttttataa tgtacaggct tcagcaggct ttggtgcttt caatagcgag
      301 gtgtatgccc ctgatgacta tattgggcta agtaaacgat ggctagatgt gcgtggcttc
      361 tatttgagct atcttgcatt tattaccgct tcaggtgatt caatgtaccc aaccatcagt
      421 gatggcgata tgctattggt aaaccgagcc accacccagc ctaaagacgg taaaatttat
      481 gtgttccgcc aaggcgagca gctttgggtt aaacgtgtgc aaggcattat gggcggtatt
      541 cgcttaatca gcgataataa agagctatac agccctattg atgtgatttt tgatgaaacg
      601 ttagattttg aggttatcgg gcaggttgtg tacattggac acgagatgat ttaaacgcca
      661 tttaaagcta cttaaatcat ttttactttt tcgttgtttt aatggtacaa gcggttagtt
      721 ttagccattt tttcgcaatt cattattttt acttttttct acataaaaca aaggggcaat
      781 gcaccgcaaa gcccctgttt tcaatacttc tgtttcactt attcccgaaa aattccgcca
      841 aattcctatt attcttttat ttcttagttt taatggttct atacagtaat gaagtagcaa
      901 acggcaaatt gcttatcact gggggaacga acctcaacgg tacgctttct gcagaaggta
      961 caagcgaaat cattttatcg ggccgtccaa cacctcatgc ctatgataaa gtagcgaata
     1021 aagaagtcct gattgaaggc gactggatca accgatcgtt caacgcaaca atctttgcag
     1081 ctaaagacaa tggcaagctt gaaatcagcc gtaatgttgc caatattaac ggtaatttta
     1141 atttaacaga taatgctacc gctcaaattg gttttaccca aggtacctca caagcatgta
     1201 ttcgctctga ccgcacaggc gtagcaacct gtaatattaa cgctacttta tcagagactg
     1261 atttaaacag ctgggagaga accaaagtgg ctggcaatgt ctctttagct gacaattcaa
     1321 cattttcgtt aggcagtaaa gcagacttaa ctggctcaat tacagctcag gagtccacta
     1381 aggttcaact taatgatgga agtattgcta atttaaccgg cttgagtaca acaggaatat
     1441 ttaatggcgc caatggttca accgttaatc tttctggggt atggacaacc ttaaccaaca
     1501 ctgttgtaga tgtcttgaat gtcgcaaatg gtagccaaat ccatttaaac actccaactg
     1561 caacaaaaca gaatacgctt accgctaata ccctcacagg gaacggcatc tttaaatttg
     1621 tcgctgatct gacgaaaaca gcgagcgata tagtcacaat taaagacacc gcaagcggtc
     1681 aattttcgat agaaattagc acaaatggta gcgcccattc agcccaaaag attaaattat
     1741 ttgaaacaac aaatgaagaa gacttttctc ttaatttagc aaatgaagtg cgctcaggcg
     1801 aatatcgcta ccatttactc aaagaaggga atgtgtatta cctcaatcca gagaaagagg
     1861 aagaacctaa aattactcaa aaagggccaa gtgaaaccgc tgacccatta cctgaataca
     1921 ccggggatat tgagcattct gaaaaagggc cgggtgaaac tgctgatccg ttacctgaat
     1981 acacaggcga cattgagcat tcagaaaaag gatcgggtga aaccgctgag ccattaccta
     2041 aatacaatgg aaatgcacat ttgatcgcat ccgagttaac tgcgcaaact aatgctgtat
     2101 taggtgtaac tacagtatta gatcatgtaa ttcattcgga agggacccaa aaacagaata
     2161 cgcttaccgc taataccctc acagggaacg gcatatttaa atttgtcgct gatctgacga
     2221 aaacagcgag cgatatagtc acaattaaag acaccgcaag cggtcaattt tcgatagaaa
     2281 ttagcacaaa tggtagcgcc cattcagccc aaaagattaa attatttgaa acaacaaatg
     2341 aagaagactt ttctcttaat ttagcaaatg aagtgcactc aggcgaatat cgctaccatt
     2401 tactcaaaga agggaatgtg tattacctca atccagagaa agaggaagaa cctaaaatta
     2461 ctcaaaaagg gccaagtgaa accgctgacc cattacctga atacaccggg gatattgagt
     2521 attctgaaaa agggccgggt gaaactgctg atccgttacc tgaatacaca ggagacattg
     2581 agcattcgga aaaagggccg ggtgaaactg ctgatccgtt acctgaatac acaggagaca
     2641 ttgagcattc agaaaaagga tcgggtgaaa ccgctgagcc attacctaaa tacaatggaa
     2701 atgcacattt gatcgcatcc gagttaactg cgcaaactaa tgctgtatta ggtgtaacta
     2761 cagcattaga tcatgtaatt cattcggaag ggacccaaaa ttggaaagta tggtctaaaa
     2821 cagattatca aacgacagag catcgctctg ccggatatgg atttaaccaa gatgcgaacg
     2881 ttatccatct aggattagag aaagccttag ataatcaggt cacacttggt atgatattat
     2941 ctcaatcaaa agcgagaaat aattttgacc attattatag cggtaaaggt cgcttaacaa
     3001 tgctatcaat gtatgcgaag aaaacatggc aaaatggtgt ttttgttgcg atagataccg
     3061 gttttgggaa agcaagcaat cgcttaactt atcaagcaaa tacagtaaaa ttagatcgtt
     3121 cagtatttgt aacagggctt agtatcggta aagcatggga atctgcaaac gtgaatataa
     3181 tcccatcatt tagcgcccgt tatcatcatt tatcttcagc aggtaatcaa cttgtagatg
     3241 caaaaatcga aacaaacgct gttgatcttc tcgctcttca agctggtctg tcaattaata
     3301 aaacgcttga actaaatggc ctgcaaataa aaccggaaat aggtagctat tttgttgatg
     3361 caagccatgg gaaattaaga acacgtttca ataatttaca aatagagcag caaatggggc
     3421 gttattttaa acaagaagca ggtatatccg ctaattatcg caatataaac gcaggaattc
     3481 aggcaggttt cttaacgggg aatacgctaa ataaacagcg ttatatctcg ttcaaagtta
     3541 catatgaatg gtaaatattg agattaaggg ctatccatta ctaaatggca tggcccttta
     3601 tttactcctc aacaatgtaa attacccatc aaaacaaact ctgctgccca agcaaattct
     3661 ccggcacagg tttgatttga aatccttttt ccgccaaaaa gccttttagg gtttgaatat
     3721 tataaaaggc gtggctttta gtggtgtttt caaagtagag atagagcctg tcaaattccg
     3781 ttctgcggct aaaaagcaat tctgccaagc cttttaattc ctcgtctgaa tagcggtaat
     3841 cgtggcgttc tgccgcactc tgccctttcc accaattcgg gttacgacca tgtaagcgta
     3901 aataagcagt acgttggttg gcaaaaaagt gaaatgccgg taagccgata ttttgtgggt
     3961 aatccacatt acaccaaatc agattagagc ttttcgcaaa ggtatcaaac accgccggaa
     4021 tatgccagct tgggtggcga aattcaatcg ctaacggata atccgcaaac cattggcaca
     4081 actcggcaag gtaacggcgg ttaagtggca tacgttcaaa ttgagttggg aactgaacaa
     4141 ataaatttgc caataaccct tcagcggtga acggagctaa cgcattaaga aataattgtg
     4201 cttgatctaa agtcgccgtg cgttgatggc tgaaatcttg atgcagtttc accgaaaaat
     4261 tcagccgacc ttccgctttc tgcaccattc cttcaaaggc tttctgcccg atgggggcgt
     4321 ggaatgtgct gttaatttcc agcgtgtcgt aatgttggct atagacaaag agaaaatcct
     4381 cttttgcagt gccgaatggg taaagcgtgc cgattaaatc tgtgtcggca tagccgccgg
     4441 tgccaatata aatatgaggg agcattgtgt ttattctttc aattttttgc aaattttaat
     4501 ggttttttaa ccgcttgcaa actgagaata aaagagtaag atttggctat cttttcttat
     4561 ttcatcatca ttatgtggat ttggtttact ctgctggcag ccgtggctca agccggtcgc
     4621 aatgcctttc aaaagcaatt aagcgtcaat gtgcctgtat tgggggtaac gctcgcccgc
     4681 tttttctatg ctgtgccgtt ggcaagcggt tatctctatt ttttatacca atatgacgat
     4741 aacctcaaca ttccccattt tccaccgctg ttttttgtat atgctatcgg ggctgggttg
     4801 gctcaaattc tggcaacttc gctaatggtg caactttttc acttaaaaaa ttatgcaata
     4861 ggtgtcgggc tggcgaaaag cgaggcagta tgggcggctt tactgggcgt gttatttttt
     4921 ggtgtaacca tcagtggcat tggctggctt ggcgtggtac taggtggagt ggcgattttc
     4981 ttaatgaccg gcagcggtaa tctacgccaa ctttcgtgga aaacactctt tctcggcata
     5041 gccagcggtt tatgtttcgc cctcacatca ctttgggtgc gagaggcaag cctgcaactc
     5101 aacagccact atttgctttc agcggcgtgg gtacttttta gcgtgattag ctttgaggca
     5161 gtgatattgg ttatatacct agctcttctc caaaaagaga cactgattaa attctttcgt
     5221 tttccaaaat taggtatgtt gaccagcctg ttttcatttc tcggctcatt cggttggttc
     5281 aatgcaatga gcctgaacca cgttgccttt gtaaaaacgc ttggacaagt ggaaattttc
     5341 tttattctcg gtatttccta ttttgtgttt aaagaacggc ttaaaattca agattttata
     5401 gggctgattt tagtagtgat tgccgcaatc ttggtggtgc tgggctagat cggattttta
     5461 cgttttttat ctagctttgt agggtgggcg taagcccacg caaaaccaat aaatcattta
     5521 tccgtgggct tacgcccacc ctacgatacc taaaaaaatt aaatttcaca tcttagtaat
     5581 aaaatgaact ataaaaatcc tcatagtatc cttgtggtga tttatgctaa aaatacccaa
     5641 cgagtattga tgctacaacg caaggacgat cctgattttt ggcaatcggt gaccggttcg
     5701 atcaatattg gagaaacacc gctggaagca gcagaacggg aagtgagcga agaaaccggt
     5761 ttgcaaactt ttgagcaaaa acaaccgctt gttgattgcc aaaagcagat agaatttgaa
     5821 attttcccgc attttcggta taaatacgca cctaatatca cacattgtgt cgaacattgg
     5881 tttttgctgc cacttgatag tgagcaagag cctattttaa ccgaacattt ggcatatcgt
     5941 tgggtttcag tggaagatgc ggttcgactg actaaatctc ccaacaatgc ccaagcaata
     6001 caagaatatt taacatcaaa ataaggaaac aacagaatgg caggccatag taagtgggca
     6061 aatattaaac accgtaaagc ggcacaagat gcacaacgcg gtaaaatttt tactaaatta
     6121 attcgtgaat tggtgactgc cgccaaactc ggcggtggtg atacctcttc caaccctcgt
     6181 ttgcgtgcgg cggtggataa agcgttatcc agcaatatga cccgtgatac catcaaccgt
     6241 gcaatcgagc gtggtgtagg cggtggcgat gataccaata tggaaaccaa aatctacgaa
     6301 ggttacggcc cgggtggtac agcagtgatg gtggaatgtt taagtgataa cgctaaccgt
     6361 acgatttccc aagttcgtcc aagtttcaca aaatgtggcg gtaacctagg tactgagggt
     6421 tcggtaggtt atttatttag caaaaaaggc ttaattttag tggcaaatgc cgatgaagat
     6481 gccttaactg aagctgctat tgaggccggt gcggacgata ttcaaccaca agaagatggt
     6541 agctttgaaa tttataccgc ttgggaagat ttaggctcag tgaaagacgg cattgaggct
     6601 gcaggcttta aaattgagaa tgcagaagta acaatgattc catcaactac cgttgaactt
     6661 gacgcagaaa ctgcaccaaa acttcttgat ttaatcaacc gccttgaaga ttgtgatgat
     6721 gtacaaaacg tttatcacaa tggtgaaatc agtgacgaag tcgctgcatt actgtaataa
     6781 aaaaatcacg caaaataagc ataatttagg ttatgcttat tttttatgaa cttttttcct
     6841 atattactga ctaacctaat gtagtttata tgctactact ttactataac attttaagga
     6901 gagtctatga aattagtgaa atttgtccct gccctagcag caactttagt attagccgct
     6961 tgctctaatg caaacacaca atctgctcct cagcaagctc aggacgctgt gactaaagtg
     7021 gctgaacaag caacacagca agtgaatgaa attgcaggta atgcacctcg tgcaagtggt
     7081 gcagtaaaag tagaaaaagc aactgatggt gcaaaatcaa ttgtatatcg ttgccaaagt
     7141 ggtaaacgtg taactgcgaa ttatgtcttc caaggagaag aggcaaaagc agtaaatcta
     7201 ttagtccaaa gcggcggtaa aacacaaaag atcccaacat taactcgtga tgcctcaaac
     7261 caagattcta cttcattcaa aacggataaa tattcttggg atttagacac aggctttacc
     7321 ttatcatcta atggccagtc aggtatgcta accaagtatg gtgctaaaac agataccatc
     7381 gttgctaaat tatgtgatgt agataaagca ttaacctcaa aattaaataa ataatctttc
     7441 ttgattttac tactcataag cgagaagata atatcttctc gcttatttta ttgcacaaag
     7501 atggctatta ttttaggaat tgaccccggc tcaagggtaa caggttacgg tgtgattcgc
     7561 caaaacgggc gacagctgga atatttagga agtggggcta ttcgaaccac ggctgaagat
     7621 ctgcctaccc gcttaaaacg gatttatgca ggcgtgagtg aaatcatcac tcaatttcaa
     7681 ccaaatatgt tcgccataga gcaggtattt atggcaaaaa accctgattc tgccttaaag
     7741 ctcgggcagg ctcgaggaac agcgattgta gcagcggtta atcacgattt acctgtgttt
     7801 gaatatgccg cccgattagt caaacaaacc gttacgggaa ttggttctgc tgataaagta
     7861 caagtgcaag atatggtaac ccgtatgctt aaactctccg ccaaaccaca agccgatgcg
     7921 gctgatgcat tagccattgc gattacccac gctcattcaa tccaacattc actgattgtt
     7981 gctaagcaaa gccagcagaa tgtgagtagt gagaaagagc aaattttagc actaatgaaa
     8041 acccgttata gccggggaag gtttaggtta aaaggctaac tcaatataaa atgaccgctt
     8101 ggaactaatg tttcaagcgg tcattttttg agaaaatttt acaattactc gccttcaaaa
     8161 ggagctgaga aacgttcaat tctcgcccca atctgacgta atttttcttc gatgtgttca
     8221 tagccacggt ctatgtggta aattctatcc acaatcgttt cacccgttgc aatacagcct
     8281 gccaacacta agctgatgga agcacgcaag tcagttgcca ttacttctgc tgattttaac
     8341 tgctctacac catagcaaat tgcaatatta ccttcaattt ccgctttggc acccatacga
     8401 atcagctcag gaatatgcat aaaacggttt tcaaaaatcg tttctgtaat acggcttgtg
     8461 ccttctgcaa ctgtatttaa taaggtaaat tgagcttgca tatcagttgg gaaacctggg
     8521 tgtggcatag tgcgaatgtt cactgctttc gggcgacgac ctagagaatc aagcgtgata
     8581 gtattttccg tcacatcaac ctgcattcca gcttcacgta atttttcgat cactgcatca
     8641 agagtgtctg ctttggtgtt acggcaagta attcgaccac cggaaatagc cgctgccact
     8701 aaaaatgtac cggtttcaat acgatccggc actacgctgt gttcgcaacc gcctaaatgc
     8761 tcaacccctt caatggtaat catatcggta cctgcaccag aaattttagc tcccatcgca
     8821 tttaagaaat cggcagtatc tacaatttca ggctcacgag cagcattttc aataacagta
     8881 gtaccttttg ctagagtacc tgccatcatc accgataaag ttgctcctac gctcacttta
     8941 tccatataga ttcttgcacc gtttaaacga ccgtttgaag ttgctttgac atagccttca
     9001 tcaagctcga ttttcgcccc cattttttca agaccggaaa tgtgcatatc taccggtctt
     9061 gccccgatag tacaaccgcc cggtaaagaa acttgcccaa gttggaaccg ggcaactaat
     9121 ggagctaacg cccaaattga agcacgcatt gttttcacta attcgtaagg tgcaacgtaa
     9181 ttattgatct tactcgcatc aatatgtacc acgcctttat cgctaccttt ttcaaccgtt
     9241 acccctaatt ggcgtaagat tttaaaagtg gtgtccacat cttttaaatc aggcacattt
     9301 tttaacgtaa ctgttttttc agccaaaata gcggcaaata aaatagggag tgcggcattt
     9361 ttcgcacctg aaatatcgac agtaccattt aaagaaaatg gaccatacac acgaaatttt
     9421 tccatttggg agatcctaaa aaataataat ttgtaggggc gaaaaatttt tcgcccaatt
     9481 ttccaacgtt tacaaaagag tttgggcgaa tagtgattcg cccctacatt tcatctatcc
     9541 aaccatattt agcaaacgct cagttttcca tttttccacg ctgaatactt taatggttaa
     9601 agcgtgaatc gcattagtag tgaaatactc agctaaaggg gcataaacag cttgctgttg
     9661 ttttactttt gaaagagaag caatgctgtc acttactaca ataacgccaa agtgggcatt
     9721 ctcgccttga gcgtggactt ctgttgcatc agataatgct tgttttaaaa tttcttcaat
     9781 ttgtgatgga ttcattctga cttcctaaaa tatgattggt aactcactca aataataaat
     9841 tgctcaaacc aaccgtctaa atcgtataat gcagccaaag ttctgacaga atctggcgta
     9901 ttaatcaaac tgtttggtat gattttatgg taatgattca gtaattcagc aagtaaggta
     9961 aaaccggcag aatcaatccg agtgatttct tttaaatccc aatataggct ttgtttccct
    10021 gttggagata aaaaagaagc acgttgcttc cataaaggga gcaacgtgtt acgagttaat
    10081 tcgcctgata gtttcacaaa aagactttcg ttattttgct gaatgtccca ttgtaatgtt
    10141 ttttgaggca taactaaact acttatttta aagtgatagg ctgttttgca gattgtgcca
    10201 cttgtgcaat taaggcatca atcccttttt gacgaatcac acccgcccat tcgttttgct
    10261 tggttgcaac catacttact ccttcagcgg ccatatcgta tgcttgccat tcacctgttt
    10321 tgctgttttt acgccattta aaatctaatt tgatcggctg tgcagcacct ttacctgaca
    10381 cgtttacacg aatgcttacg atagatttac catccaccga tttcgggctt tcaatttgca
    10441 cactttgatc ttgatactgg gttaacacct gagcataaga ctgctcaaca aattgaccaa
    10501 aagcattaaa gaatgcctct ttttgtgcat cagtcgcagc cgataagttt ttccctaaca
    10561 ctaattggcc tgcatatttc acgtgaacgt gtggcattaa atcgttacgc acaatggtac
    10621 gcaaatattc agggtttgat ttaattttcg cttgatttgc tttaatatcg ccaaataatt
    10681 tatctgcggt ttgttgcatt aatacataag ggctagtttc agcaaatgcg gtggttgaaa
    10741 ataatgcaga aactgccact aagcctgtta cgataatttt tttaatgttt tttaacatta
    10801 ttgatgctct ccaaaactct aaattagagg ttgttgtact taattactct gcttttggtt
    10861 cagcagaatc acttgctttt tcatcttcag ttttatctga ctttttatca ccatataaga
    10921 actgaccgat taaatcttct aacaccattg cagaattggt atctacaaaa gtatcacctt
    10981 ctttcatcat tgcagtttca ccttcgatta cgaagccaac atttaacgca acgtattgct
    11041 cgcctaataa gcctgaggtt ttaattgaga gcgagctggt atcgggaatt tgattaaaac
    11101 tttcatttac cgctaatgcc actttcgggg tgtaggtttt gttatcaagt gaaatatcag
    11161 ccactcggcc aactaccaca ccgccaactt taataggcgc acgaaccttt agtccaccaa
    11221 tattatcaaa agttgcatac agggtgtaag ttttctcatt tgagaagccc tgcacatttg
    11281 ctactcttaa gcctaaaaag acaagagctg caagcccaag tagtacaaat aagcctaccc
    11341 aaaattcata tttaattgat tgacgcattg tctgttcctt tcaaaaaatc aaccgccgaa
    11401 cattatcgca gttaaaataa aatccaatcc taaaatcacc agtgaagcat ttaccaccgt
    11461 tcttgtggtc gcttggctga ttccttctga agtcggtacg caatcgtagc cgttaaataa
    11521 ggcaatccag accaccgcaa aggcaaaaat cacactttta ataaagccgt taattaaatc
    11581 gctggtattt accgagtttt gcattaccga ccaaaaactg ccaccatcaa cgcctttcca
    11641 atctaccccg actaatgagc cgccccaaat tccgatagcg gtaaaaatag cagctaacac
    11701 cggcattgaa atcacgcctg cccaaaagcg aggagcgatg atacgtctaa gcggatcaac
    11761 cgccatcatt tcaagactgg aaagttgctc tgtggctttc atcagcccga tttctgcggt
    11821 caatgccgaa ccggctcgcc ctgcaaataa taatgcagta actacaggac caagttctcg
    11881 taaaagcgag agtgaaacca aagttcccaa gctactttcg gcggcaaaat cgaccaatac
    11941 cacatagcct tgtaagccta acaccattcc gataaataag cccgacagca taataattaa
    12001 gagcgattgc actcctaaca cataaagctg tttaatcagt aacggggtat gttttctaaa
    12061 ttcaggttta ccgattaatg ctccccataa cataaaagcg gaacgcccta atgtacggat
    12121 aaaattaatc accaccgccc cgatagagct aataaaatta accattaaat agctcctccg
    12181 tataatcttt tgctggataa tggaaccgca ccggaccatc agctttaccg gctaagaatt
    12241 gtaccacttg cggatcttcg cttgcctgta aaatttccgg tgtaccttca gcaatcactc
    12301 gtttatctgc cacaatataa gcataatcgg caatacttaa tacttctttc acatcgtgag
    12361 acaccacaat ggaggtcaaa ttcaatgcct gatttaactc tttaatcagc tcgacaatca
    12421 cacccatact aatcggatct tgcccggtaa acggctcatc atacataatc agatccggat
    12481 ctaacgcaat cgctcgagca agggcggcac gtcttgccat accgccggaa agttcggacg
    12541 gcattaaatt tgccgcacct cgcaacccaa ccgcctcgag cttcaataac accaatttgc
    12601 gaattagctc ttcgggtaaa cgggtatgct cccgaatcgg gaaagccaca ttatcgaagg
    12661 tagaaatatc ggtaaataac gcccccgatt gaaacaacat ccccattcgt tggcgaattt
    12721 catacaactc tttgttattg gctttgcaaa tatctctgcc atcaaataaa atttcgcccg
    12781 attcaggtaa aatctgccca ccgattagcc tcaacagggt ggttttacca atcccagaag
    12841 gccccataat ggcagtcact ttacctttct gcacttgcag attcaggtta tcgtaaatca
    12901 ctcgctcccc tcgcttgaag gtcaagtttt ttacttcaac caaattttcc gtttttgtca
    12961 tcaaatagga ctcaaattaa taaaagtgat atttatggtc tatcaaattt gccagaatgt
    13021 caaaaaactt ttaataatct gacaaaaatc tgcctttaca agcggtcaga tttgctaaat
    13081 tttttgcaaa ccggctgaac tgaaaaagaa aggtcttgtc ttagccaata cgagcgtttt
    13141 cactcgtaga ttataaaaac ataggatatg aggaacattc acaatatgaa caaaacaatg
    13201 aaaaaatcat ttttaactgc attcatttta ggctcaacca ttgccgcaat gccggcgtat
    13261 gcagaagcaa cattacccac agccgtaaat gggcaagctg tgccaagcct tgcaccaatg
    13321 ctggaaaaag ttcgcccggc ggtggtgagt attgcagtag aaggtaaaac caaaggcgat
    13381 agccgccgca gtcgtgaaat tccggaagaa tttgaatttt tcttcggtcc tcacgccgat
    13441 ttattcggca accgctcatc agctccacgc aatttccgtg gcgttggatc aggtgcagtc
    13501 atcaatgccg aaaaaggtta tgtgattacc aataaccacg tgatcgacaa tgccgataaa
    13561 attaccgtaa aacttgaaga cggacgtgaa tttaaagcta aattagttgg tgctgatccc
    13621 ctttcagatg tggcgttaat tcaagttgaa gatccaaaaa acttaactga aattaaattc
    13681 gccgattccg ataaattaca agtgggcgat tttacagttg caatcggtaa cccgtttggc
    13741 ttaggacaaa ctgttacttc aggcatcatc tcggcacttg gtcgttcaac cggtcaatcc
    13801 gatgaaggct atgaaaacta tattcaaacc gatgcggcag ttaaccaagg taactctggc
    13861 ggcccgttaa ttaacttaaa cggcgaatta atcggaatta acaccgctat tatttctcca
    13921 agtggtggca atgccggtat cgcctttgcg attccaagta atatggcaaa cagcttggtg
    13981 actcaaatta ttgagtttgg tgaagtaaaa cgtggaatgt taggcattaa aggcggtgaa
    14041 ttgaatgctg accttgctaa agagttcaat atttccaccc aacaaggggc atttgtaagc
    14101 gaagtcttac cggattctgc tgctgctaaa gccggcttta aagcaggtga tgtgattact
    14161 gaaatcaacg gtcaaaaaat ccgtagtttc agcgaattac gagcaaaagt agctacttca
    14221 ggtgtaggta aagagattga acttacttac ctgcgtgacg gcaaagaagc caaagcaaaa
    14281 gtgaccttac aatcggattc tgaagccaaa gttactgctt caaacctcat tccggcatta
    14341 aaaggggcag attttaacaa ctacaatgcc aaaggcatta aaggggttga aatttctaac
    14401 gtagaaaaag gctcgattgc tgaaatgcga agcctcaaaa aaggcgatat tatcatcggc
    14461 gtgaaccgcc actcggtaga aaacattgcc gatttacgca aaatattaga aaccaagcca
    14521 tctgtggttg cactcaatat tgtgcgtgac ggtgcaaatt tctatgtgat tttgcaataa
    14581 accttttgat ccactcaaac aaaagccgat gacaaaaatc atcggctttt gttattgctg
    14641 ggaatttaac atctgtcgtg ccatttccat actttggata atcgctctgc ggttgataga
    14701 atcattattg ctatctaaca ctttacgcca attatcgaga gcttgttgaa aattgttatt
    14761 taaaaagctg tcggaggcta gcaggagtaa actggcattt tcatttttat ccaactgtag
    14821 ggcttgctcg ataatggctt gaatttcaac ggtcattttc tgcccgttat cgtaataacg
    14881 agcggtggca atcgccccca aaatagccgc tcgttttccg agtagcttct cggcattatc
    14941 gtagcaaacc agtgccgaat caaaatcatt gcttaacgca taagcctgcc ctagttcgta
    15001 ccataaatca ccgttattcg ggttttctcg aagctggttt tgcagactca aaatatagcg
    15061 ttcatttttc gatgtgccgt gtgcatcgct catctgttgt tgaaaatctt gatgttgttg
    15121 caaacctgat tgcaccactt gataacgtcc ggaaagccca tagtaagtca ttgctaacaa
    15181 caaaatcgcc acgcaactca ggcttgcgat tttagcaagc ggtcgatttt gagcattatt
    15241 ttgcaaaacg ctctgttcaa attgcgttcg ttcaaattgg taacgttgct ctgcctcacg
    15301 ctcaaactct tgtctgacgt catctgcaaa atgttgggca aatttaaccg cttgttggta
    15361 gttttcttgg cttaattgct cacgcgtttt catttttgtt gcctttattt ttgcgagttc
    15421 gccacaaaat agctagaaat aacaggctaa acaagccaac cggcaagccc cataataccc
    15481 atgtctgtgg cgtgaatggt ggatcgtaat tcacaaaact gccgaaacgc tcggtcataa
    15541 tgcggataat ttcctcatta cttttccctt gattcaccat ttcatacact tcaattcgca
    15601 tattgtaagc cgccgtcgca ttggattcga ccaaattttg gttctgacat tgcaaacatc
    15661 gcaacgaacg agccagtgaa atcgcccgta cccgatccgc ctcattttga aattgaaagg
    15721 tgtccaccat ttcagcttga accgtgccga caaatagcaa caagccaaat acaagcggta
    15781 atattttgcg gattttttgc aaaaagccga tggaaattat gctatttctc atttctgaga
    15841 gcctctaatt tgggtaggaa atcctgtgca aaatcagggt tgtaaccctt ttgttggtaa
    15901 cgcactacac cgtgtttatc aatcaaataa ctggaaggtg cagaagagac tttcagggtt
    15961 tggatcaaaa agcctttttc gtaatcatca ataattaaac taaacggatt tccccagttt
    16021 ttcaatgcgt tgagggcatc atttgggcgg tcgctataag ttaagccgac aatcggcacg
    16081 cctttttgtt ttagttccaa caaaatcggg aactctttaa tgcaccaagt acaccagctc
    16141 acccacacat tgagaatata aggctctttc ggcaagctgt tgttatttaa atgcgcgtta
    16201 ctatcaagta agttcttgcc tacaaactct ggaaacggct tgccctgcca atcttcagtg
    16261 ggagcaaggg catctttatt cattaacggc acagtcaaaa agccaacaat aactacaagt
    16321 aacaggagcg gaataagtaa caggattttt ttcatttttt gttttctcta cgataaccga
    16381 ataatgccaa cagggcggcc attaccatca tcataccacc taaccataag gcttgcacat
    16441 aaggcttata gtgcaaacgg aaggcatatt ctaaattgcc gaacttatcc cccatcacaa
    16501 tataaatatc gtctaaattg tggtggaaaa gccctacttc tgccattgtc atcgttctta
    16561 catcataata gcgtctttcc ggtgtgacgg ttgccagcaa ctgttcatct cggctgatag
    16621 aaaaaacggc ttgctcggcg gtgtagttcg gaccgatgag atcgtgataa tcttcatatt
    16681 taaaggtata accggcaagt tccgctgctt gattcggctt taaccgcacg ccgatttcat
    16741 cgccgtaata actgttcatc attgccccaa tcacgcaaac cgcaaagccg atatgggcta
    16801 accgcatcgc aagcggtcga atttgccaca ttttttgcga atagggcaaa tgagtgagaa
    16861 taatccagat cgcaaggctg ataaatatgg tgggtaacgg ctgcaaagca aaatgctctc
    16921 tttgcgagat ggaggcataa atcaacccta acgctaaacc gactgcaatc ggcagcaacc
    16981 aaagttttac caatatctgc ttatttgaaa tctgtttcca gcccagcacc accgcaaagc
    17041 ccattactat cattaacagt aacgctagtg gcgtgaatac gctattgaaa tagggggccc
    17101 ccactgaaat cgatccccaa ttcatcactg tgaagatcat cgggtagaaa gtccccagca
    17161 ataccacaga agcagccaca ctaaataagc cgttaatcaa taaaaatgcc gtttctttag
    17221 aaagtaagtt gaaacgcact ttgccttgcc agagattcgc tcggaatgca aacagagcaa
    17281 gggcaatgaa acttaagccg aagaataaga tcagcagtgc cattcctctg tcgggatcga
    17341 ccgcaaaggc atgaaccgag gtcagtacac ccgaacgaac aataaacgtg cctaataggc
    17401 ttaaagcaaa ggcgaaaatg gcaagtagaa tcgtccaata atagaaaatg ccacgctgtt
    17461 ggctcacaat taaactatgc accaatgcgg tgccaagtag ccaaggcatt agggaggcat
    17521 tttccaccgg atcccaaaac caccagccac cccagccgag ttcgtaatat gcccaccacg
    17581 cccctaaaat aatgccggcg gtgagaaatc cccacgaaat catcgtccac ggacgaagcc
    17641 aacgcataat cgcagcgtcc attccgcctg aaacgagggc ggcgacaatc attgcaaagc
    17701 tcaccgcaaa gccgacatag cccaagtaga gcaaaggcgg gtgaaagatg agcccaatat
    17761 cttgcaacat tgggttgaga tctcgccctt caggtggtgg tggaaagcca cgttcaaatg
    17821 ggctggaaac caatagaatg aaaagtagaa aacctagtgc aattagccct aacatcgcta
    17881 aggttcggtt ggcaaacaag cggtcaattt tttgcgaaaa tttgcaaaat accgcagacc
    17941 aaatcacaag agcggtgagc caaaacagca ttgagccttc gtgtccgccc caagtggcgg
    18001 caaatttaaa aaaatcgggg agctggctgt tggaatgagc ggcaacgtag ataaccgaaa
    18061 aatcatcggt tagaaaagca taagctaagg tagaaaagga aaatgtggta aaagcagctt
    18121 gcaaataagt gaaaagcgga gccaatgcca gccaatgata ttgcttgcga acttcgccta
    18181 gaagtgcaaa accaacttga aaaatggcac taattgcagc caataaaagt gaaaaatagc
    18241 cgagttccgg tagcataaaa aatccaaaaa atgaccgctt gtgaatgtaa aaaaagccct
    18301 ctccacttgt gggagaggga cagatttttc ttcgttcaga agaaaaatca gggagagggg
    18361 aattaaataa ttttgctaaa aataacaata ctccctctct ctgaaaaata tttctgaacg
    18421 aaatattttt cggtctcaat actcgctacg ctcgctcctt cggagccgtt ggcaaagcca
    18481 acgttcaaat gtaaacattt gtcccacaag gggagagagt atatcctaac ttgcaaccgt
    18541 tagttgccct gcataaagga taaagtaacg caagcagagt acaccgatta agtcgaaaac
    18601 ggaaattaag ataataaatt tcacgttatg tttcaagcgg tggctaccaa aaatattggc
    18661 aacaagcgga atcgcaatac cgattaacat tacgcctatc cagaacacat tcccccagaa
    18721 gccgttaagt gcatgggtta aagcgagcga tttatctgct ccaccgaagt gtaagcccac
    18781 aaagaacgct acaattaagc ctaattcggt taccataatc ggcacttcaa atttgtgcat
    18841 aaagtggact tcgtggcttt caccggataa cttgcctgcg agtaaaatca ctaggaaagt
    18901 tgctgcaata cctgacgatg cccctgatgc caagaacaat gccggtaaca cagggttatt
    18961 cagcattgga taactcacta acgccgagag taagaaaccg gtgtaagcac caagtacagc
    19021 agccaatacg aataagataa attcaattgg ttttaacagc ttcgcttcgg caaaatcgat
    19081 caaaccagag accacaggta gataccgaac aatccaatct ttaaacatta tggcgatcca
    19141 aaccaccaat actgccatat aaagttggaa taacattacc cccatcgaca ttactgaggt
    19201 aaagttatag ttgaacatta atttccagaa tgtccaaggt ttggttaagt ggaaaatcag
    19261 taacagtaag ccgataatgg ttggtaatgt gccaagaata gccgcactac gaataatcca
    19321 gttttggctt ggtttttcca ttttgacacc gctattacgg taagcaatcg ctaactgaac
    19381 cgcacctgat gaaataccaa gtaggaataa gtaaatagcg atagtgccat cccaaacaag
    19441 gtttggggtt tggaaaggta catattcatt cattatctat gttctccttt accaaatgga
    19501 atgtgatata aattcggctc cgtgcctaat gccgttttgg tgcgataaac cggattattt
    19561 ttcactttat ggaaaatatc gctacttgga tcattcatat caccaaaggt taaagcttta
    19621 gtcggacaag cctctacaca agcaggctgt ttgccttctg caagattggt atctcggcaa
    19681 aagttacatt tatccgccga ttttttctca gggtgaataa aacgtacacg atacgggcaa
    19741 accgcaatac agtattggca accgacacac aaatctgagt gtacatcaat aatcccggtt
    19801 tcaggatcga taaatgaagc accggtcggg caaacactca cacaaggtgc attggtacag
    19861 tgttggcaag attggcggaa aaactcatat tgtacgttcg gaaactcacc gaaaggctca
    19921 ctacggataa tttctaaacg agaaacccct tccggcactt tgttggtggt gcgacaagcg
    19981 tccatacaag ccgtacagcc gatacaggca gtttcatcat gaaccatcgc atagcggatt
    20041 ttcttttgtt ctttctcagt agcaagagtt atggaggtgg caagccctgt acttgccaca
    20101 actgcgatag cacctgcccc tgagacgaaa tctctacggg tgcaagtcat tattttgttc
    20161 cttttgcttc tgtggctttc atttcaggat ttttaaattc gcctgaatga attttggtat
    20221 gacaatccgt acaaagttta atgcgatctt tttcaggaat gtctttcatc ggatcttttt
    20281 ccgggtgaat ttcatgacag ttggtacaag caattttagt tgcgtgcgtg tcgtgagccc
    20341 agaatttttc acgcaatttc tctgccgtat ggcaagcaaa acagacttga ttttgctcat
    20401 ttaccgaacg ttcaagtgat gggttacttg ctttaccgtg tgggttaaaa cgcattacgt
    20461 cttttgcccc tttgcggtgg ctttcagaaa tattaccgtg gcaacttata caagtgagtg
    20521 gctctccatt attcgggctt ttcacatctt tttggaaatg tttgcctgcg tgatggaaag
    20581 gcttacctgt tgcgtttgcc gattcgtgac attgagtaca atatttattt ggatcacggc
    20641 gtgcatcttg agctactttt gcatcatcag ccgtttctga ttgccaaaga ggttttggct
    20701 gctcaggcat taacgcattc gcactggaaa ttgcaaaaaa acctgcaaat acgaccgctt
    20761 gtatgagggc gactgctcgt cctgcttttg agattaattt tttcatagtt cacctcaagc
    20821 tacattattt tgcttctgct tttggtgctg ctaccggttt gtctgcttct tctgcgggta
    20881 ataaaccttt agcacgagct tctttttccc attgaggaac cacagttttt aagaactcat
    20941 cttttgctgc ttgctcttta ggaatatcta aaccaatagc aagttgtgct ttctctttag
    21001 ttgagatgtc cggaatcgcc actggcgttg ttacaccgtg tttcgctaac actacaccta
    21061 atttagcacg ggcatctgct gcacggtcga tacctgttgc gatggtacgt aacattacat
    21121 caggtgcgtg gaagtggtta ccgtgacctg ccgcagagta atcccaacgc cattgagcgt
    21181 gacgaattgc cattaagatc tctttcatct cttcttgagt tgctccagct tcccaagcag
    21241 ctttcgcttc aaagtgagct ttcactaatt gatcttctaa gcgaatcatt gcctctttta
    21301 cttcgtgttt atactctttt acacgttttt ccaatgtttc tttgctttgt tcatgacaag
    21361 ttttacaagt cgcttcaaat tggtcaaacg gattaccgat tttgtggtca gtataaactt
    21421 tgccgtcttt gtctttcact tttggcatat ggcaatcaat acaggttaca ccatttttcc
    21481 catgtgtacc ttgtgaccaa acttcaaaat ctgggtgctg cgtttttagc atcggagctt
    21541 tcgataaagc atgagtccag tctgtaaaat tgatttcatc gtagtatttt tcaatgctat
    21601 ctacatctac acctttatcc caagggaaag ttacatctgt tttatttttg aaatagtact
    21661 ccacatggca gttagcgcac acagcagggc gtttaccatg tttatctaaa tcctcaaact
    21721 tccaaccaac cgtattcaat gcacgtaata cgtgaggacg tgcaacacgt agtgcaggtt
    21781 taccttgttt aaactcttct gatgtcgtgt catggcagtc tgcacaacct attgagttta
    21841 caatttcagc accatatttc gcccatttag gatcaaaata tcctcgctca cctttctcct
    21901 caattaagcg tggaacatca gggcctttac atgtccaaca agccataggt tgtggaccat
    21961 cattttcatc tttaggcgcg ccggtgcgca aaatatcacg cacatcggta actgcataaa
    22021 agtgacctcg tggtttatta taatctttcg caaaagcata gcctgcccac aaaataacat
    22081 aacgagggtc agcctctaac gcacttcctc gatctgtgga ttttgcagtt gacttccaag
    22141 aatcgtattg tagcggatat ttttctgcaa ataggtggtt tgcagactca attttcacgc
    22201 ctaatttttc atgcttaact gctttatctg ttaatgattc agttttcgcc tcttcagcta
    22261 aagcagtttg taaacacccc aatattgaaa ttgttgccac agctaggctt tttattaaaa
    22321 atttcatttt ggctctccca atcaggtaaa aataattaga gaaaaataag aatttttcct
    22381 attaaaatta ataagataat ccctgatatt cagttgcgag aata
//
LOCUS       DANLXS010000016        22340 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.016, whole genome shotgun sequence.
ACCESSION   DANLXS010000016 DANLXS010000000
VERSION     DANLXS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22340
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.016"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(76..975)
                     /gene="cdd"
                     /locus_tag="PXD71_000513"
     CDS             complement(76..975)
                     /gene="cdd"
                     /locus_tag="PXD71_000513"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL6290670.1"
                     /translation="MFRTDITERFEQLKAAKNCEVTQAIIEQLIKQDFHGQLSYEVVD
                     ELCEKFKRSRVELALYCIAIAACYAVTPVSDFNVGAVAIGKNGDFYFGANQEFSGECM
                     QQSVHAEQSAISHAFLAGETLITDVVVNYTPCGHCRQFMNELNSAASLKVHLPHSQNN
                     LLHSYLPDSFGPKDLGIEKVLFDEQPQQFEVKGDHLQQAAIFAANNAYAPYSNAFSGV
                     ALQVGERIVCGKYTENAAFNPTFLPLQSALNYRLFLGLADIPVTRVVLAEAKGSLSSY
                     AMTQALAKSLFGLEIEYLSLQAV"
     gene            complement(989..1891)
                     /locus_tag="PXD71_000514"
     CDS             complement(989..1891)
                     /locus_tag="PXD71_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6290671.1"
                     /translation="MISSSLKLGYFSLIVATLFWGGNFVLGKVLSGAVQPITLTYLRW
                     LPAFLILVLLFFRPTWRVMHLLKPRLGVMWVLGLLGVILFPATLYEGLKTTTALNASL
                     YLAISPVLVLFLNRLVFKEKISPIILSGAIISLLGVLWLLMQGKLTRLFSLAINQGDL
                     WAIASTISWAVYCCVIRLRPQGISNTVFLTVLVGLAVLTLTPFFIYEYVAESAKNLAN
                     LTALQWSGIGYLVIGPSILSYAFWNFGIAIVGSAKGAVMTNFTPLFAAMFSIILLNES
                     VHTYHIISAILITAGILICSYKKH"
     gene            complement(1888..2244)
                     /gene="folB"
                     /locus_tag="PXD71_000515"
     CDS             complement(1888..2244)
                     /gene="folB"
                     /locus_tag="PXD71_000515"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDL6290672.1"
                     /translation="MSDKVFIRELTAFASIGAYNWEHTIKQRLVFNIDMAWDFSKAAE
                     TDDVQYCLNYAEVSQKVLDFVESQPFKLVETVAYQLAELLQKEYQLSWIRIELHKPKA
                     VAQASSVGVIVERGHQ"
     gene            2314..2919
                     /gene="plsY"
                     /locus_tag="PXD71_000516"
     CDS             2314..2919
                     /gene="plsY"
                     /locus_tag="PXD71_000516"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL6290673.1"
                     /translation="MNLIPYLLILTAYLLGSISSAVIFCKLAGLPDPREVGSHNAGAT
                     NVLRIGGKSAALGVLLFDILKGTLPVAMGIYFELSPSQLGFIALAVCLGHIFPIFFQF
                     RGGKGVATAFGAISALSFIVAGGAIGTWLLVFLISGYSSLSAVFTALVVPFYIWWFEP
                     QFTFPVALVCCLLVYRHHDNIQRLWRGQEDKLWDKFKKNRA"
     gene            complement(2993..4900)
                     /gene="parE"
                     /locus_tag="PXD71_000517"
     CDS             complement(2993..4900)
                     /gene="parE"
                     /locus_tag="PXD71_000517"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL6290674.1"
                     /translation="MSEKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNS
                     VDEALSGFADKIDVILHADNSLEVVDNGRGMPVDIHSTEKISGVELILTKLHAGGKFS
                     NKNYTFSGGLHGVGISVVNALSERVEIQIKRGGEVYSIAFANGVKVEDLTVIGTCPKK
                     QTGTTVRFYPNPKYFDSPRFSVSRLRHLLRAKAVLCPKLTINFIDKINNTEESWYYED
                     GLSDYLSEALNGYETVPNPPFIGDVTAETEAVSWALTWLPEGGELVAESYVNLIPTAQ
                     GGTHVNGLRNGLLKAMVEFCEIHNLLPKGVKLTADDVWNRCAYVLSLKIQEPQFAGQT
                     KERLSSRQASSYVDSTLKDAFSLWLNQNVQTGKLIAEMAISSAQSRMRAAKKVVRKKL
                     VSGPALPGKLADCTAQDLSRTELFLVEGDSAGGSAKQARDKEYQAILPLRGKILNTWE
                     VSSDQVLASQEVHDIAVALGIDPDSDNLEELRYGKVCILADADSDGLHIATLLCALFL
                     RHFPKLVQNGHVYVAMPPLYRIDIGKDEVHYALDEAEKEAILARLAKKKGKPNVQRFK
                     GLGEMNPSQLRETTMEPSTRRLVQLTFDDTVEEGELNTFEIMDMLLGKKRAEDRKQWL
                     QNRGDEADLAV"
     gene            5108..5332
                     /locus_tag="PXD71_000518"
     CDS             5108..5332
                     /locus_tag="PXD71_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL6290675.1"
                     /translation="MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEE
                     REGSRIAVVLFQQVRVFHRPHPNPDTDKGR"
     gene            5375..5704
                     /locus_tag="PXD71_000519"
     CDS             5375..5704
                     /locus_tag="PXD71_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL6290676.1"
                     /translation="MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQL
                     KKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSPELHKAAVIAATAENLSLNEWIN
                     QTLEKSV"
     gene            complement(5743..6129)
                     /gene="cueR"
                     /locus_tag="PXD71_000520"
     CDS             complement(5743..6129)
                     /gene="cueR"
                     /locus_tag="PXD71_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDL6290677.1"
                     /translation="MNISQAAEKTGLSAKQIRDYEKAGLLPPTSRTEAGYRFYNATDL
                     SRLHFISNARKVGFSLAQISELLKLNDNPNRSSRDVKQLTEQHIGELEQKIADLNQML
                     TLLKSWSSSCCGNANSECSILNGLNG"
     gene            6218..6418
                     /locus_tag="PXD71_000521"
     CDS             6218..6418
                     /locus_tag="PXD71_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL6290678.1"
                     /translation="MTITLQLDGLHCGNCVKSVEKALNGVAGVNQVSVTLEPQIAVVE
                     GSAEAQALIEAVEDIGFDAKLA"
     gene            6471..8663
                     /locus_tag="PXD71_000522"
     CDS             6471..8663
                     /locus_tag="PXD71_000522"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL6290679.1"
                     /translation="MTSTAHYSPEQQLLIDGMHCAACVRRVEKALLKVEKVEFASVNL
                     ADQTAFVQGNANPEALVAAVVKIGFGAEVLESEEERRIKQQAQTQRVLGHKRTQFMLA
                     LVVGFALVAFGFYKGMTVNETNRSLWLSWAGISLATMYFSGKDFFLGAFSALKNRSAN
                     MDMLVAISTGTAWLYSCWLTLFPQPNAHVYFEASVMILGFINLGKYLELKAKQRSSLA
                     LEKLLDLAPKQAVIFEENIAKTIPAKAIKPQMRVQALTGDRLAVDGILDSGSIWVDES
                     MLTGEALPVEKKIGDKVRAGTLVQDGSGTYIAEQVGSQTALARVINAVRHAQSSKPPL
                     AQFVDKIAAVFVPVVVSIALLSALIWLAVGQDFAFALSIFTTVLIIACPCALGLAIPL
                     STIAGVARSAEFGVLVRNIEALQASSEIDTLVFDKTGTLTTGRMEVSDVLTFNGFEQK
                     RLLQLAKSVEQHASHPIAKAIAKFAENQTACGVTDVQVLKGLGISAKYEGSTIRMGNW
                     QFVGETIQANLASLLPAKEGDFSSKIFVSVDNQLAGVLSVSDQLRPEAKATIHQFQAE
                     GYQCVMLTGDRQQTADYFANALGLDGVIAEVLPEQKAEKIRALQAEGKKVAMIGDGIN
                     DAPALAQANVGIAMHNGSDIAVETADLSLMREGLAPLMQILPFSKKVLKNMKQSLLGA
                     FIYNVIAIPIAAGVLYPFTGWLLNPMIAAIAMTLSSITVVLNSQRLLK"
     gene            complement(8716..9501)
                     /gene="truA"
                     /locus_tag="PXD71_000523"
     CDS             complement(8716..9501)
                     /gene="truA"
                     /locus_tag="PXD71_000523"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL6290680.1"
                     /translation="MKIALGIEYDGSRYFGWQRQEAVESVQQQLEQALSVVANSPVEV
                     FCAGRTDAGVHGTGQVVHFETQANRPLQSWCFGTNAHLPDDIAVKWAVAVNEDFHARF
                     SATARRYRYIIFNQKLRSAILPKGVTHFHFPLDERKMHQAGQYLLGENDFSSFRAAKC
                     QSNTPWRNIHHLNVSRQGNYVIVDIQANAFVHHMVRNIVGTLLEIGQGRQPVEWAKWV
                     LEQRDREKAAPTAKAEGLYLVEVHYPEHFGIPKTPLGPLFLAD"
     gene            complement(9566..10255)
                     /gene="folE"
                     /locus_tag="PXD71_000524"
     CDS             complement(9566..10255)
                     /gene="folE"
                     /locus_tag="PXD71_000524"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDL6290681.1"
                     /translation="MTTISPELTITENTLSPEAQKVRQALLAKGIETPTVTHTKDKAS
                     RRAEIQQHMQAVLELLGLDLSDDSLEETPHRLAKMYVDEIFSGLDYATFPKITNIENR
                     MKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQV
                     QERFTEQILTAFQTILETDDVAVYVKATHFCVKCRGIKDTNSYTVTSAFGGVFLEDRE
                     TRKEFLGLLNK"
     gene            10356..11063
                     /locus_tag="PXD71_000525"
     CDS             10356..11063
                     /locus_tag="PXD71_000525"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HDL6290682.1"
                     /translation="MQNIQYLFNADLIPEHQAEFVQSLLTCKNFDNSDRLLGFSKGRG
                     TTWFLDTSAELGINVVLRHYYRGGLFGKIIKDSYFFTRFEATRAYKEFFLLQQMLAWG
                     LPVPRPVAIKIERHFCCYRADIMLEKLENTQDLSKILQSHSLSNEQYEQIGKLIRSLH
                     NHQIHHSDLNIHNILLGNDGKFCLIDFDKCGIEPGENWKQNNLDRLLRSFKKEQGRLN
                     IFFSEENWQAVLRGYTL"
     gene            complement(11118..12215)
                     /gene="apbC"
                     /locus_tag="PXD71_000526"
     CDS             complement(11118..12215)
                     /gene="apbC"
                     /locus_tag="PXD71_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDL6290683.1"
                     /translation="MNQLNEQQLVEIKAILQQFSHPTLQKDLIALNAFKKAELGGDIL
                     RIEITMPFAWNSGFETLKAETEAKLKQVTGSSGVKWVLNYQIATLKRANKHPAVNGVK
                     NIIAVTSGKGGVGKSTTSVNLALALRAQGTRVGILDADIYGPSIPHMLGAKDQRPTSP
                     DNKHITPITAHGLQSNSIGYLMEEDNATIWRGPMASSALSQLLNETWWNELDYLVIDM
                     PPGTGDIQLTLSQQIPVTGAVVVTTPQDIALLDAIKGISMFEKVSVPVLGVIENMSMH
                     ICSNCGHHEAIFGTGGAEKIAQKYGTKVLAQLPLHIRLREDLDSGVPTVVAAPEHEIS
                     QAYLALAEKVASELYWQGSVIPSEIMIREVK"
     gene            complement(12212..13759)
                     /locus_tag="PXD71_000527"
     CDS             complement(12212..13759)
                     /locus_tag="PXD71_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6290684.1"
                     /translation="MLKSLQAVTFVKKIAKVTACILLITSCDQLICPQSIQSVWVENR
                     QSDTLLKRAIYSAVFQLDPHKIKASADAAPLRDLLVGLTAYNRKGDVVPAIAQNWFTE
                     DEKEWLFILDEKAVWSNGQPVTAQDFVASWRRLIDPQNHSVLSKYLIYMGIENAKAIE
                     EQIKSVEELGVEALNAHTLRIRLNHPNHQLPEMLAHSALLPTYQGIAPTIDNFVSNAS
                     YRLEKLDAQKMVLKARTQEIPFQTIEYDLITTIQNCSAFDIVENPLLNKKTNIVKFPR
                     LCSYYYEFNFDDPLLAKREIREAIRTMVASANSGQEYGVPSQSVIPHSLVKDREKRWN
                     PVVVEQLLTKAGISPYNPLKLTLLYDENSINIANQVTRALSQSELFNITPKMVDWQTL
                     NKVREQKDYQIARAGWCADYPDPIVFLQKFHSMSPDNHSGYKSEIVDQKLNRLQTETL
                     SAEARESLIQEISEQLYSDVAVLPLFQYHHRVGIVSSLLGIDLNNDSEVIYSKDLKRN
                     LANKDNQ"
     gene            complement(13785..14435)
                     /locus_tag="PXD71_000528"
     CDS             complement(13785..14435)
                     /locus_tag="PXD71_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HDL6290685.1"
                     /translation="MDIIINFAIYLQFFIGLFAIVNPFGSLPIFFSMTAHQYEGERNR
                     TSLITAVSIGVILLVSLYFGNLILNAFSISLSSFRVAGGILIVSIAMTMISGKLGEHK
                     QNKEEKNEDITEYENIGVVPLAMPIMAGPGAIGSTIVWGTRYHTWEDYVGFSIAIIVF
                     ALICYILFRYSAPLVKKLGKTGSNVVTRIMGLILMALGIEIIVAGITTLFPVLLVK"
     gene            complement(14557..16428)
                     /locus_tag="PXD71_000529"
     CDS             complement(14557..16428)
                     /locus_tag="PXD71_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HDL6290686.1"
                     /translation="MIEKMHERTNGPVFKIIFALVSISFVIGGIGTGLISQDNSVAKV
                     NGEEISQQLFNNTLNREQNRLNAELGSRFWDLMDSPEYAAQFNQSVLNGLIDEELLRQ
                     YAKELKLGISADQIKSEIVNSQMFQQDGKFSNELYQQTLRHNNLSADGYAAIVQEGML
                     QSQLQEGIINSDFNVPAQQALLAKLLLQQREVRLAEFSVAKEMENQTASEQELRAYYE
                     ANKTKLLAPEKLAVEYVTLSPKDVESKVEITNEQIQTYYDRNKADYVTKGEAHLAHIQ
                     VASEAEAQAIEQALKNGEDFATLAKNKSSDTLSANNGGDLGWAKAGTFPKAFEDAVAN
                     LTAGQMSAAVKVDNAYHIIKVLERKPEQVIELAQVKDKIAQTIRQELVLTEYFTVARE
                     MANKAFENNSSLEEVAKAGNVELQKTDLFTRENVPTALQNEKVLKVLFEGELRQNKQN
                     SDALDVGNDANPQTLFVRVSDYDAERPQTFDEAKAAVENFVKAEKAEKALLAKVEENV
                     KALNEGKSVDVKFNAPQTLVYMQAEVENPVLAGTIFAMKKAQDKTTYQMARNQKGDVV
                     IVALDNITEGNAEQFKTLAPQFEQANRLILRNEFLQSLRAKASIEVNEDFMQQRSSH"
     gene            16610..16879
                     /gene="rpsO"
                     /locus_tag="PXD71_000530"
     CDS             16610..16879
                     /gene="rpsO"
                     /locus_tag="PXD71_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL6290687.1"
                     /translation="MSLSVEAKAKIVAEFGRDAKDTGSSEVQIALLTAQINHLQAHFA
                     EHKKDHHGRRGLLRMVSRRRKLLDYLKRTDLAKYSETIARLGLRR"
     gene            16951..17820
                     /locus_tag="PXD71_000531"
     CDS             16951..17820
                     /locus_tag="PXD71_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDL6290688.1"
                     /translation="MAFASLFTLLDDIAAVLDDVSIMTKVAAKKTAGVIGDDLALNAN
                     QVSGKNVVPERELPIVWAVAKGSLINKVILIPLALLLSVFLPQAIIPLLMIGGAYLCF
                     EGVEKLLHRLLHKNNQAHSDETETLSEKDKIKGAIRTDFILSAEIIIIALGVLQDSST
                     LTQILSLSAIGVGITLFVYGLVGLIVKLDDIGLWLMQKKSTFSQAVGKGLLFAMPWFM
                     KGLSVIGTIAMFLVGGGIFTHNLPMIHHFVEQFGISPHFSTLVDFAVGIVVGIIACLI
                     ILPLMKLFSKKAH"
     gene            complement(17910..19175)
                     /locus_tag="PXD71_000532"
     CDS             complement(17910..19175)
                     /locus_tag="PXD71_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HDL6290689.1"
                     /translation="MFRTLFLMLLLLVGLISGPYLAGNQGYVRIETATTVIEMSIVML
                     VVFFVLAMAVVYLLEWIVTKICRMSNGAYNWFSTRKHKKAQQETLEGLMRMSEGNYSK
                     AEKLFSKNAKHADEPVLNLIKAAEAAQQNGDDLAANKYLIKATEIAGPNNVAVELVRT
                     RILLQQGKLPAARTAIDSLLELAPHSDEVLRLAIQIYKESRAYVALDRLLVEIGQRNF
                     LTAEEYAELEYFVDDGLQDERLQEEGQEGLLSWWENQPNRRRKSVYSRVGVIKRLIDS
                     DDHQSAQEIALETLKKFEDEQLQGLFVQLTRLQIDSDSKLLKVLAKRADKANVKYTDD
                     YARALGYIYTRDGQYQKAKSQFELLLAHDECVADDRIMALHVAEQMGDTLMANRIREE
                     NLKQVNMATQSKVNEPLALPQTATESNSL"
     gene            complement(19194..20504)
                     /locus_tag="PXD71_000533"
     CDS             complement(19194..20504)
                     /locus_tag="PXD71_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDL6290690.1"
                     /translation="MSRSKKVTEHLEHSAEKVVEEAQQAVDSAGSFAKKEIEEDVVNE
                     AIEKDIQTEMQKAPKEQVVVQKSGGKGLALVALLVALAVGGAGHYMANKKFAEVESQI
                     QAVSAKANQPTTQTMVEMPNFENEKAQIAELVTEYQKALERIKALETAQNGYTQQISS
                     LQLQLQKLNNTSGSDNTFWLLSEADFLLNNAARKVVLDNDIETAKNLLLEADQVLSKA
                     PNTIHIREAIKTDLNTLTALHQIDQNALMQRVANLANKLDDLPILESEQASNSKQDGQ
                     VSDSLADWEKNLEKSAASFLDHFIRVSKRNAADEKAFVAPNQEIYLRENIRLRLQIAI
                     LAVPRQQSELYKKSLQTVGSWVRSYFETSNENVQHFLKELDELAEQTIYVDAPTSLQS
                     LKVIAQQLNKTPEKIEKVEIKAEKELEQAEPVKAEQDAMPLSAQ"
     gene            complement(20516..21280)
                     /locus_tag="PXD71_000534"
     CDS             complement(20516..21280)
                     /locus_tag="PXD71_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDL6290691.1"
                     /translation="MNVLVTRLDSRGQELVDMLNEQQIFAIHQPLFTIEAGKELPLLP
                     SAISRLKSDDYVFVVSPNAVDYAAKTLVDTGFHFRSDLKYFAVGQRTAKYLTEKAEQA
                     VIYPIEFENSEGVLALPEMQNLTDKTILILRADSGRELLAETAVLRGATIQYLECYRR
                     EPVTDDIPEKISLCKRLGVDTIVITSSEILRSLYEQAKADCRAWLFECDLVVVSRRIA
                     KIAKQMGWQEDKIRVSDKADNVTLMNTLLFKVGKSN"
     gene            complement(21280..22215)
                     /gene="hemC"
                     /locus_tag="PXD71_000535"
     CDS             complement(21280..22215)
                     /gene="hemC"
                     /locus_tag="PXD71_000535"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDL6290692.1"
                     /translation="MKDILRIATRQSPLALWQANFVKSELEKHFPQLSVELVTMVTKG
                     DIILDTPLAKIGGKGLFVKELELALLENRADIAVHSMKDVPMSFPEGLGLAVICERED
                     PRDAFVSNNYHSLEDLPKGAVVGTSSLRRQCQLMAKYPHLEVKSLRGNVGTRLSKLDN
                     GEYDAIILASAGLIRLGLQERIRSYISVEQSLPAAGQGAVGIETRANDERILNYIAKL
                     NHSSTACCVMAERAMNTRLQGGCQVPIGGYATLEGDELTLNALVGSLDGSQIIRASAK
                     GHKQEAEQLGILVAEQLLAQGADKILAAVYNENVQ"
BASE COUNT         6394 a         4932 c         4181 g         6833 t
ORIGIN      
        1 gaagctcatc aaaaaattta acactaaaaa ataaattgct taaagataca gcacctttgc
       61 aaaaataaat caaaattaca ccgcttgtag cgaaaggtat tcaatctcta acccaaatag
      121 tgattttgcc aatgcttgag tcattgcata actactgaga gagcctttcg cctcagccag
      181 cacaactcga gtaacgggaa tatcggcaag ccctaaaaat aatcgatagt tcaacgcgct
      241 ttgcaacggt aaaaaagtag gattaaaagc tgcattctca gtatatttac cacaaacaat
      301 acgctcacct acttgcagtg caaccccact aaatgcgttg ctgtaagggg cataagcatt
      361 attggcagca aaaattgccg cttgttgtaa atgatcgccc ttaacctcaa attgttgcgg
      421 ttgttcatca aataagactt tctcaatccc taaatctttc ggtccaaaac tatccggcaa
      481 atagctatgg agcaaattat tttggctatg cggtaaatgt acttttaagc ttgccgcact
      541 gtttaattca ttcataaatt ggcggcaatg cccacaaggg gtatagttca caaccacatc
      601 tgttatcaaa gtttcccctg ctaaaaatgc gtgcgagatt gcactttgct ctgcgtgaac
      661 ggattgttgc atacattccc cggaaaactc ttggtttgcc ccaaaataga aatctccgtt
      721 tttgccaatc gcaaccgccc ctacattaaa atcggaaact ggtgtaaccg cataacaggc
      781 agcaatggca atgcaatata aagccaactc aacacgagag cgtttaaatt tttcacagag
      841 ttcatcaacc acttcgtaag agagctgccc gtgaaaatct tgcttgataa gttgctcaat
      901 aatagcttgt gtaacttcac agtttttcgc tgcttttaat tgttcaaaac gctctgtaat
      961 gtctgttcga aacatactgt acctccgttt aatgtttttt atagctgcaa atcagtatcc
     1021 ctgccgtaat cagtatggca ctaataatat ggtaagtatg aactgattca tttaataaaa
     1081 taatactgaa cattgctgca aagagcggcg taaaattagt cattaccgct cctttagcag
     1141 aaccgactat tgcaatgccg aaattccaaa acgcataaga gagaatagaa gggccgataa
     1201 ccaaatagcc tatcccactc cattgtaaag cggtcaaatt tgctaaattt tttgcagatt
     1261 ctgctacata ttcatagata aaaaatggcg ttaacgttag aactgccagc cccactaata
     1321 cggtcaaaaa caccgtattg cttatacctt gtggacgtag ccgaatcaca caacaataaa
     1381 ccgcccagct tatagtagaa gcaatcgccc ataaatcacc ttgattaatt gccaagctga
     1441 ataagcgagt tagcttccct tgcatcaata gccacagcac tcccagcaaa ctgattattg
     1501 ccccacttag aataatcggg ctgattttct ccttaaaaac gagccgattt aaaaatagca
     1561 ccaatacagg cgaaatagct aaatagagac tcgcattcaa tgctgttgtc gtttttaaac
     1621 cttcatataa ggtagccggg aataaaatta ccccaagcag acctaatacc cacatcacac
     1681 ctaaacgagg ttttaataag tgcattaccc gccaagttgg gcgaaaaaag agcaaaacta
     1741 aaattaaaaa agccggcaac caacgcaaat aggttaaggt aataggctga actgccccac
     1801 ttaacacttt gcctaataca aaattcccac cccaaaaaag agtagcaacg attaaactga
     1861 aataccccaa tttcagtgat gatgagatca ttgatgacct cgttctacaa tcacacctac
     1921 actgcttgct tgggcaaccg ctttcggctt atgcagttca atccgaatcc aagaaagttg
     1981 atactctttt tgtaatagtt ccgccagttg ataggcaaca gtttcaacta atttgaaagg
     2041 ttgagattca acaaaatcca gcactttttg cgatacttcc gcataattca agcaatattg
     2101 tacatcatcg gtttctgccg ctttggaaaa atcccacgcc atatcaatgt taaaaaccaa
     2161 gcgttgctta atcgtatgct cccaattgta agccccaatg gaagcaaaag cggttagttc
     2221 tcgaataaaa accttatcac tcatttttcg tcctgtttta ttttctgaat ttctgtaaaa
     2281 tatagcacaa gataaattta atcaggaaaa tttatgaatt taattcccta tttattgatc
     2341 ttaaccgcct atttgctcgg ctcgatttcc agtgcggtca ttttttgtaa gctggcaggc
     2401 ttgcctgacc caagagaagt cggctcacac aatgccggtg caacgaacgt actacgaatt
     2461 ggcggtaaaa gtgccgcatt aggtgtgctt ttattcgata ttttaaaagg cacattaccg
     2521 gtggcgatgg ggatttattt tgaactctct ccctcacaac tcggtttcat cgccttagcg
     2581 gtttgtcttg ggcatatttt cccgattttc ttccaattcc gtggcggaaa aggcgttgcc
     2641 accgcatttg gtgcaatctc cgcattaagc tttattgttg caggcggtgc tatcggcact
     2701 tggctactgg tgtttttaat ctccggctat tcttctttaa gtgccgtctt cactgccttg
     2761 gtcgtaccgt tttacatttg gtggttcgag ccacaattta ctttccccgt cgccttagtt
     2821 tgttgcctat tggtttaccg ccatcacgat aatattcaac gcttatggcg tgggcaggaa
     2881 gacaaactgt gggataaatt caagaaaaat agagcataaa aataggcata gtgaaccgtt
     2941 cgctatgcct atttgcaaat tattgcggaa aaatcaccgc ttgtcttctc gactaaaccg
     3001 ctaaatctgc ttcatctcct cgattttgta gccattgttt acgatcttcc gctcgttttt
     3061 tacccaatag catatccata atttcaaagg tattcaattc gccctcttct accgtgtcat
     3121 caaaggttaa ttgtactaag cggcgagtgc ttggctccat cgtagtttca cgcaactggc
     3181 tcgggttcat ttcgcctaag cctttaaagc gttgcacatt tggctttcct tttttcttgg
     3241 caaggcgagc taaaatcgcc tctttttccg cctcatctaa ggcataatgc acctcatctt
     3301 tgccgatatc aatgcgataa agtggtggca ttgccacata aacgtgtccg ttttgcacca
     3361 atttcgggaa atgacgaagg aataacgcac acagcaaggt ggcaatatgc aaaccgtctg
     3421 aatccgcatc ggcaagaata caaactttgc cataacgcaa ctcttctaaa ttatcgctat
     3481 cagggtcaat tcccaaggct accgcaatat cgtgtacttc ttgtgaagcc aatacttggt
     3541 ctgatgaaac ctcccaagtg tttaaaatct taccacgcag tggcaaaatg gcttgatact
     3601 ctttatctcg agcttgtttt gccgaaccgc cggcagaatc accttctacc aagaaaagtt
     3661 cggtacggct gagatcttga gctgtacaat ccgccagttt gcccggtaaa gccggaccac
     3721 tcaccaattt cttacgcacc acttttttcg ccgcacgcat acggctctgg gcggaactaa
     3781 ttgccatttc agcaattaat ttacctgtct gcacattttg gttaagccat aaactaaagg
     3841 catctttcaa ggtgctatcc acatagcttg aggcttgacg ggaagacaaa cgctctttgg
     3901 tctgccctgc aaattgtggc tcttgaattt tcagagaaag cacataagca caacggttcc
     3961 acacatcatc agcggttaat tttacccctt tcggcaataa attatggatt tcgcaaaact
     4021 ccaccatcgc ctttaataaa ccgttacgca agccattaac gtgcgtgccg ccttgtgcgg
     4081 tcggaatcag gttcacatag ctttccgcta ctaactcgcc cccttccggc aaccaagtta
     4141 atgcccagct taccgcctct gtttcagcag taacatcacc aataaacggt ggattcggca
     4201 cggtttcata accgtttaat gcctcgctca aataatcaga taagccgtct tcgtaatacc
     4261 aactttcttc ggtattgttg attttatcaa taaaattgat ggtgagtttc gggcaaagca
     4321 ccgctttggc tcgtaacaaa tggcgtaaac gactcaccga aaaacgaggg ctatcaaaat
     4381 attttgggtt cgggtaaaaa cgcactgtcg tgccggtctg ttttttcggg caagtaccaa
     4441 ttaccgtcag atcttccact tttaccccgt tggcaaaggc aatcgaatag acttctccgc
     4501 cacgtttaat ctgaatttca acccgttcgg acagggcatt taccaccgaa atccccaccc
     4561 cgtgcaaacc accggaaaac gtatagtttt tattggaaaa tttaccgccg gcgtgcaatt
     4621 tcgttaagat aagctccacc cctgaaattt tctcggtgga atgaatatcg accggcattc
     4681 cccgcccgtt atccaccact tccaacgaat tatcagcatg aagaatcaca tcaattttat
     4741 ctgcaaagcc ggacaacgcc tcatcgacac tattatcaat cacctcctgc cccaaatggt
     4801 ttggacgtgt ggtatccgtg tacattcccg ggcgaagctg caccggctcg aggtctttca
     4861 gaacggtaat ttcatccgcc ccatagcgtt tttcagacat tatgctttct cttaggctta
     4921 aaataaaata aggctgaatt ttaaggaata ttcagctatt ttgcaaaatt ttggtgaaaa
     4981 atgacctgtt gttatgcaat aaaaactatt tttgcgatct acatcgaaaa aatgagtttt
     5041 aatttcccta tttgcttatt cattcagtat cttaatctca ctttttattt taaaatggta
     5101 ctatattatg gtagtaaaaa tcctaaagcg gaaatctcaa aagacactac aacaaatcta
     5161 cacccagcct atttctgcca atattaaatg gacaaatata gaagctcttt ttctagagtt
     5221 aggagcagaa atagaagaac gagaagggag cagaattgca gttgttttat ttcaacaggt
     5281 cagagtattt catcgcccac acccgaatcc tgatacagat aaggggcggt agcgagtatt
     5341 aaaaaatggt tatctgaaaa cggagtcatt ccttatgcgt aatattatgg atattaatgg
     5401 ttataaagct gtcattgctt acgatcccga aacagaatta tttcgaggtg aatttatcgg
     5461 attaaatggt ggagccgatt tttatgccga taatgtcatt caattaaaaa aagaaggtga
     5521 actaagtttg aatatctttt tagaactttg tagagaaaaa ggtattgagc cgttcaaaca
     5581 atattccgga aagtttaatg ttcgcctttc gcctgaatta cataaagctg cggtaattgc
     5641 agcaactgct gaaaatttaa gcttaaatga atggattaac caaacgttag aaaagagcgt
     5701 gtagcctaat tgcaaaacaa tcacctattt tgaccgcttg tattacccgt ttaacccatt
     5761 caatatcgaa cactccgaat tggcgttgcc acagcaagag ctactccagc ttttgagcaa
     5821 agtcagcatt tggtttaaat cggcaatttt ttgttcaagc tcaccgatat gctgttcggt
     5881 taattgtttt acatctcggc ttgagcggtt tgggttgtcg tttaatttaa gtagttcgct
     5941 gatttgagcc agcgaaaagc ccacttttcg ggcattgctg ataaaatgta atcggctcaa
     6001 atcagtagca ttgtagaatc gataccccgc ttcggtgcga cttgtcggcg gcagaagtcc
     6061 tgctttttca taatctcgga tttgtttggc ggaaagcccg gttttttctg cggcttggct
     6121 aatattcatt ataatcctct cactttaatc gcttgacctt aacctaaggt taagctttat
     6181 tatacgcctg aataatcact caaaacagga gaaaatgatg acaattaccc tacaacttga
     6241 tggcttacat tgtggcaact gtgtgaaaag tgtcgaaaaa gcgttaaatg gcgtggctgg
     6301 ggtgaaccaa gttagcgtaa cgttagaacc gcaaattgct gtggtggaag gctcagcaga
     6361 agcacaagcc ttaattgaag cggttgaaga cattggcttt gacgcaaaat tagcctaatt
     6421 aataaggggc agaaattttg ccctaatctg cccaatattt cggaggataa atgacaagca
     6481 cagctcacta ttcgccagaa caacaacttt tgattgatgg aatgcactgt gccgcctgtg
     6541 ttcggcgagt ggaaaaagcc ttacttaaag tggaaaaggt ggaatttgcc tcagtcaatc
     6601 ttgccgatca aaccgctttt gtgcaaggca acgccaaccc tgaagccttg gtagcagcgg
     6661 tagttaaaat cggttttggg gcagaagtgc tggaaagcga ggaagaacgc cgaattaaac
     6721 aacaagccca aacgcaacga gtgttagggc ataaacgcac tcaatttatg cttgcccttg
     6781 tagttggttt tgccttagtt gcttttgggt tctacaaagg aatgacggta aacgaaacca
     6841 atcgttcgct ttggctcagc tgggcaggta ttagccttgc gacaatgtat ttttcaggta
     6901 aagatttctt tcttggggca ttttcagcac tgaaaaaccg ctcagccaat atggacatgt
     6961 tagtagcaat cagcaccggc acggcttggc tctattcttg ttggctcacg cttttcccac
     7021 aaccgaatgc tcacgtttat tttgaagcca gcgtgatgat tctcggcttt atcaatctag
     7081 gaaaatactt agaactgaaa gccaaacagc gttcctcgct agcattggaa aaattgctgg
     7141 atctcgcccc aaaacaagcg gtcatttttg aagaaaatat tgcaaaaacc attccggcta
     7201 aagcaattaa gccacaaatg cgggtgcaag ccttaaccgg tgaccgcttg gcggttgatg
     7261 gtattttaga cagtggctct atttgggttg atgaatcaat gctcaccggt gaagccctac
     7321 ctgtagagaa aaaaatcggt gataaagtgc gagccggcac tctggtgcaa gacggttcag
     7381 gcacttacat tgccgaacag gtcggctcac aaaccgcttt ggctcgtgta atcaatgccg
     7441 ttcgccacgc ccaaagcagc aaaccgccat tggcacagtt tgtagataaa atcgccgctg
     7501 tttttgtgcc ggtggtggtt tcaatcgcat tactttctgc gttaatttgg ctggcagttg
     7561 ggcaggattt tgcctttgca ctctcgattt ttactaccgt gttgatcatc gcttgccctt
     7621 gtgcattggg gctggcaatt ccgctttcta ccattgccgg cgtggcacgt tctgccgaat
     7681 ttggcgtgct ggtgcgaaat attgaggcac tacaagcaag cagtgaaatc gacaccctag
     7741 tgttcgataa aaccggcaca ctcaccactg gcagaatgga agtgtcggat gttctcacct
     7801 ttaacggatt tgagcaaaag cgcttgctac aacttgctaa aagtgtagag caacacgcct
     7861 cacacccgat tgcgaaagcg attgcaaaat ttgccgaaaa tcagaccgct tgtggtgtaa
     7921 ccgatgtgca agtgttgaaa gggttaggca tttcagcaaa atatgaaggc agtacaatca
     7981 gaatgggaaa ttggcaattt gtaggcgaaa ccattcaagc aaatcttgcc tccctattgc
     8041 ccgctaaaga gggggatttt tcctcaaaaa tttttgtttc ggtggataat caactcgccg
     8101 gtgtattgtc ggtttcagac caacttcgcc ctgaagcgaa agcaaccatt catcaattcc
     8161 aagcggaagg ctatcaatgc gtgatgctga ccggtgatcg ccaacaaact gccgactatt
     8221 tcgccaacgc attggggtta gacggcgtga ttgcggaggt attaccggag caaaaagcgg
     8281 aaaaaattcg agccttgcaa gcagagggta aaaaagtagc aatgattggc gacggcataa
     8341 acgatgctcc agctctggct caagccaatg tgggcattgc gatgcacaat ggttcagata
     8401 ttgcagtgga aaccgccgat ctttcgctga tgcgagaagg gctagcacca ctgatgcaaa
     8461 ttctaccgtt ctctaaaaaa gtgctgaaaa atatgaagca aagtttgctc ggggctttta
     8521 tctataatgt tattgcgatt cctattgctg ccggcgtgct ttaccctttc accggctggc
     8581 tactcaaccc aatgattgcc gccattgcga tgacattgtc gtcaattacc gtggtgttaa
     8641 atagtcaaag gttgttgaaa taatttaagc ggtcaaattt gcatattttt ttgcaaattt
     8701 gaccgcttgt aatcactaat ccgccaaaaa cagcggccca agaggtgttt tcggaatgcc
     8761 aaaatgctca gggtaatgca cctcgaccaa atatagcccc tcggctttcg ctgtaggggc
     8821 agctttttcc cggtcacgtt gctccaacac ccattttgcc cattccacag gctgtctgcc
     8881 ttgcccgatt tcgagcaatg ttcccacaat attgcgaacc atatgatgta caaaggcgtt
     8941 ggcttgaata tcaacaatca cataattgcc ttgccgagac acatttaaat gatgaatatt
     9001 ccgccacggc gtattagatt ggcatttggc ggcacggaaa gaggaaaagt cattctcgcc
     9061 cagcaaatac tgccctgctt ggtgcatttt gcgttcatca aggggaaagt gaaagtgggt
     9121 aacaccttta ggtaaaattg ccgaccgcag tttttggtta aaaataatat agcggtaacg
     9181 gcgggcggtg gcactaaaac gggcgtgaaa atcctcattc accgctaccg cccatttcac
     9241 cgcaatatca tcgggtaaat gagcgttagt gccgaaacac cagctttgta gcgggcggtt
     9301 tgcttgcgtt tcaaaatgca cgacctgccc tgtgccgtgc acaccggcat cggttctgcc
     9361 ggcacaaaac acttctacgg gcgaattggc aacaaccgac aacgcctgct ctagctgttg
     9421 ttgtacactc tctaccgctt cttgtcgttg ccagccaaaa taacggctgc cgtcatattc
     9481 aatacctaat gcgattttca ttttaatacc ttgataaaaa gaggggcgaa aaatttttcg
     9541 cccccacagt aattgatatt tgcaattatt tattcaataa gcctaaaaat tctttgcggg
     9601 tttctcggtc ttctaagaaa acaccgccaa aggcagaagt aactgtatag ctattggtgt
     9661 cttttatgcc acggcatttc acgcaaaaat gggtggcttt cacatacacc gcaacatcgt
     9721 ctgtttctaa aatagtttga aaggcagtta aaatctgttc ggtaaaacgc tcttgcacct
     9781 gcggacgttg ggcaaaaaac tgcacgacac ggttgatttt cgagagtcca atcacccatt
     9841 ttttcgggta ataggctacc gctactttgc cgtcaatagt aacaaaatga tgctcacagg
     9901 tagaagttaa agtaatatcg tccaccagca ccatttcact gactttcatt cggttttcaa
     9961 tattggtaat tttcgggaag gtagcataat caagcccgct gaaaatttca tcaacataca
    10021 ttttcgccaa acgatgaggc gtttcttcta agctgtcatc acttaaatct aaaccgagca
    10081 attctaaaac tgcctgcata tgctgctgga tttctgctcg gcgactggct ttatctttgg
    10141 tatgagtaac agtcggagtt tcaatgcctt ttgccaacaa ggcttggcga actttttgag
    10201 cctccggcga aagcgtgttt tctgtgatag ttaattcggg cgagatcgtt gtcattggtt
    10261 ttcctctcaa atgtaagctg aagattctaa caaattgaca tcaaaaaaaa tagctctaaa
    10321 atgagctatt tcagccataa acaccctctt tatttatgca aaatatacag tatctcttta
    10381 atgccgacct gattcccgaa catcaagctg aatttgtgca gtctctctta acttgcaaaa
    10441 attttgacaa ttccgaccgc ttgctaggct tctcaaaagg gcgaggtacg acttggtttt
    10501 tagatacctc tgctgaacta ggtatcaatg tggtgcttcg ccattattat cgtggcggct
    10561 tgttcggtaa aatcatcaaa gatagttact tttttactcg ctttgaggcc acccgagcgt
    10621 ataaagaatt tttcttactg caacaaatgt tagcttgggg tttacctgtg cctcgccctg
    10681 tggcgattaa aatagaaaga catttttgtt gctatcgtgc cgacattatg ctcgaaaagc
    10741 tagaaaacac acaagattta agcaaaatct tgcaatccca ttctttatcg aacgaacaat
    10801 atgagcagat agggaaactg attcgcagtc tgcataacca tcaaattcac cattccgact
    10861 taaatatcca taatattttg cttggtaatg atggcaaatt ttgtctgatt gattttgata
    10921 aatgcggcat tgagccgggc gaaaattgga agcaaaataa cctagaccgc ctgttacgct
    10981 cgtttaagaa agaacaaggg cgattaaata ttttcttcag cgaagaaaat tggcaggctg
    11041 ttttgagagg atatactctc taagaaacaa taatggcaca agcttagaaa cttgtgccat
    11101 caaatcatca atattaacta cttcacttca cgaatcataa tttcagacgg aatcacactg
    11161 ccttgccagt aaagttcaga agctactttt tccgctaatg cgaggtaggc ttggctgatt
    11221 tcgtgttccg gagctgccac caccgttggc acaccgctat ctaaatcttc acgcagacga
    11281 atatgtagtg gtaattgtgc taacacttta gtgccatatt tttgggcgat tttttccgct
    11341 ccgccggtgc cgaaaattgc ctcatggtgt ccgcagtttg agcagatatg cattgacata
    11401 ttttcaatca ccccaagcac tggtactgag actttctcaa acattgagat ccctttaatc
    11461 gcatctaata aggcaatatc ttgtggggtt gttactacca ccgcaccggt caccggaatt
    11521 tgctgtgaaa gcgtaagctg aatatcaccc gtacccggtg gcatatcaat gaccaagtaa
    11581 tccaattcat tccaccaagt ttcatttaaa agctgactta acgcactgct tgccatcggg
    11641 ccacgccaaa tggtggcgtt atcttcttcc attaaatagc cgatagaatt agattgcaag
    11701 ccgtgagcgg taatcggcgt gatgtgcttg ttatcaggag aagttggacg ttggtctttt
    11761 gctcccaaca tatgcggaat agatgggccg taaatatccg catctaaaat ccccactctt
    11821 gtcccttgtg ctcgtaaggc taaagcaaga ttaactgagg tcgttgattt ccctacccca
    11881 ccttttcctg aagtgaccgc aatgatattt tttaccccat tgactgccgg gtgtttattc
    11941 gcccgtttta aggtcgcaat ttggtaattt aatacccatt ttacaccgct tgaacctgtt
    12001 acttgcttta atttagcctc tgtttcggct tttaaggttt caaaaccact gttccacgca
    12061 aatggcatcg tgatttcaat tctaagaata tcgcccccta attctgcttt tttaaacgca
    12121 ttaagggcaa ttaaatcttt ctgtaaagtc gggtgggaaa attgttgcaa gattgcttta
    12181 atttcaacaa gttgctgttc atttaattgg ttcattggtt atccttgttt gctaaattgc
    12241 gttttaaatc tttgctataa attacttcgc tatcgttatt caaatcaata cccaaaaggg
    12301 aggaaacaat cccaacccga tgatggtatt gaaataatgg caatacggca acatcgctat
    12361 aaagctgctc gctaatttct tgaataagtg actctctcgc ttctgcacta agcgtttctg
    12421 tttgtaaacg atttaatttt tgatcgacta tctcactttt atagccggaa tgattatccg
    12481 ggctcattga atgaaacttc tgcaaaaata caatcggatc aggataatct gcacaccagc
    12541 ctgctcgtgc tatctggtaa tctttttgtt cccttacttt atttaaagtc tgccaatcaa
    12601 ccattttagg tgtaatatta aataattctg attgagaaag ggctcgagta acttggttag
    12661 caatattaat gctattctca tcatataaca aagtcagctt taaaggatta taagggctaa
    12721 ttcctgcttt tgttaatagc tgctcaacca caacaggatt ccaacgcttt tctctatctt
    12781 ttactaagct atgtggaatt acagactgac tcggaacccc atattcttgc ccactatttg
    12841 ctgaagccac cattgtcctt attgcttcac gaatctctcg tttagctaat aatggatcat
    12901 caaaattgaa ctcatagtaa taagagcaca aacgcggaaa tttcacgata ttcgtttttt
    12961 tatttaataa aggattctcg acaatatcaa aagcagagca gttttggata gttgtaatta
    13021 aatcatactc tatagtttga aagggaattt cttgggttcg agcctttaac accatttttt
    13081 gagcatctag tttctcgaga cggtaagacg cattgctcac gaagttatct atggttggag
    13141 caatcccttg ataagtaggt aataatgcgg aatgtgccag catttcaggc aattggtgat
    13201 ttgggtgatt tagccgaatt ctcaatgtat gcgcatttaa tgcctctaca ccgagttctt
    13261 caactgactt tatttgctct tcaatcgctt ttgcattttc tatacccata tagatgagat
    13321 atttagacaa tacagaatga ttttgaggat ctattaaacg tcgccagctt gctacaaaat
    13381 cttgtgcagt cacaggttgg ccattagacc acacagcttt ctcatctaaa ataaacaacc
    13441 actctttttc atcttcagta aaccaattct gagcaatggc cggaacgaca tcaccttttc
    13501 tgttgtaggc cgttaaaccg actaatagat ctcttaaagg agcagcatct gcagaagctt
    13561 taattttatg tgggtcgagt tgaaatacag cagaataaat tgcacgtttg agtaaagtat
    13621 cactctgccg gttttctacc cagacagatt gaatggattg agggcaaatt aattgatcac
    13681 aagatgtaat aagcagaatg caagcggtaa cttttgcaat tttttttaca aatgtaaccg
    13741 cttgtaagga tttcaacata aactccccaa aggttaatta agatttattt tacaagaagc
    13801 acaggaaata aagttgtaat tcctgccaca ataatttcaa ttcccagcgc cattaaaatc
    13861 agccccatga tacgagtaac gacattagat cctgttttgc ctaatttttt caccaaaggg
    13921 gctgaatagc ggaataaaat ataacaaatt aatgcaaata caataatcgc aatgctaaag
    13981 ccaacatagt cttcccaagt atggtagcgg gttccccaca caatggttga gccaattgcc
    14041 cctggtcctg ccataatcgg cattgctaat ggcacgacac cgatattttc gtattcagta
    14101 atatcttcat ttttctcttc tttattttgt ttgtgttcac ctaatttacc gctaatcatc
    14161 gtcatcgcaa tgcttacaat caaaatgcca ccagctactc taaaagagct aagtgaaatg
    14221 ctaaacgcat tgagaatcag attaccaaaa tagaggctga ctaataaaat tactccaata
    14281 gaaacagcgg tgattaagct ggtgcgattt ctttccccct catattggtg agcggtcata
    14341 ctgaaaaaaa taggcaagga accaaaagga ttgactatag caaatagacc aatgaagaat
    14401 tggaggtaga tggcaaaatt aataatgata tccacaaaaa ctttctcaaa caacagtgtt
    14461 tcgggcgcta ttatatcggt taatcaaggt agatacaaac caaaagcacc cagcctaaag
    14521 gacggttggg tgctttttga taaaatgaaa ggcttgttag tgtgacgaac gttgctgcat
    14581 aaaatcttca tttacctcaa ttgaagcttt tgcacgtagc gattgtaaga attcattacg
    14641 taaaattaaa cgattagctt gttcaaattg tggcgccaat gtcttaaact gttcagcatt
    14701 cccttccgta atgttatcta aggcaacaat aactacatcg cccttttgat tacgtgccat
    14761 ttgataagtc gttttatctt gcgccttttt cattgcgaaa atcgttccag ccaataccgg
    14821 gttttctact tctgcttgca tataaactaa agtttgtggc gcattaaatt tcacgtccac
    14881 tgatttccct tcattcaagg cttttacatt ttcttctact ttcgccagca acgctttttc
    14941 cgctttttct gccttcacaa aattttcaac tgctgctttt gcttcatcaa aagtttgtgg
    15001 acgttctgca tcataatcac tcactctcac aaacaatgtc tgtggatttg catcattccc
    15061 aacatctaaa gcatcagaat tttgtttatt ttggcgtagc tctccttcaa acaaaacttt
    15121 taatactttc tcattttgta aagcagtagg tacgttttca cgggtaaaca gatccgtttt
    15181 ttgcagttcc acattaccgg ctttcgcaac ttcttcaagt gaagaattat tctcaaaggc
    15241 tttatttgcc atttcgcgcg caactgtaaa atactcagtt aacactaatt cttggcgaat
    15301 agtttgggca attttatctt tcacttgtgc taactcaatc acttgctcag gtttacgctc
    15361 caatactttg atgatatggt aagcattatc cactttcacc gctgcactca tctggccggc
    15421 agttaaattt gctacagcat cttcaaaagc tttcgggaaa gtgccagctt ttgcccaacc
    15481 tagatcacca ccattatttg ctgataaagt atcgctagat ttgttttttg ccaaagtagc
    15541 aaaatcttcc ccatttttga gcgcttgctc aatagcctgt gcttccgctt cactagcgac
    15601 ttgaatatga gctaaatggg cttcaccttt agttacataa tcggctttat tgcgatcata
    15661 gtaagtttga atttgctcat tggtaatctc aactttgctt tccacatctt ttggagaaag
    15721 agtaacgtac tcaaccgcta atttttcagg tgctaataat ttagttttat ttgcttcgta
    15781 ataggctcgc aattcttgct cggaagcggt ctgattttcc atttcttttg caactgaaaa
    15841 ttcggctaaa cgcacttcac gttgttgcaa cagtaatttt gctaacaatg cctgctgtgc
    15901 cggtacatta aaatcactat tgataatccc ctcttgtagc tgggattgca acataccttc
    15961 ctgcacaatc gctgcatagc catcggcact taaattattg tggcgtaatg tttgttggta
    16021 taactcgtta ctgaatttac catcttgttg aaacatctgg ctgtttacaa tttcagattt
    16081 gatttgatca gcactaatac ctagttttaa ctcttttgca tactggcgca gaagctcttc
    16141 atcaattaat ccatttaata ccgattgatt aaattgagca gcatactccg ggctatccat
    16201 taaatcccaa aagcgactcc ccagttcagc atttaatcta ttttgctcac gattaagcgt
    16261 gttattaaat aattgctgac taatttcttc cccatttact ttcgctacag aattatcttg
    16321 tgaaatcaat cctgtcccaa ttccaccgat tacaaatgaa atagagacta atgcaaaaat
    16381 aattttaaag accggcccat ttgtccgctc gtgcattttt tcaatcattt aaaaattcct
    16441 aaattactca aaaattgtcg ctgattatac tctttctcaa atgagaaata aacttgcaaa
    16501 tttttattga aatttaaccg ctttctcttt aaaattcaca gtccctgtcg tagctgagtt
    16561 agagatcggc tattgatgta ttatcaaccc ttaacaaaag gaaattaaaa tgtctctaag
    16621 cgtagaagca aaagcaaaaa tcgttgctga atttggtcgt gatgcaaaag acactggttc
    16681 ttctgaagtt caaattgcat tattaactgc acaaatcaac cacttacaag cacactttgc
    16741 tgagcataaa aaagatcacc acggtcgtcg tggtttatta cgtatggttt cacgccgtcg
    16801 taaattatta gactacttaa aacgtactga tcttgctaaa tattcggaaa ctatcgcacg
    16861 tttaggttta cgtcgctaat ttcaaatatt agatatgaga gccgagctga aaagttcggc
    16921 ttttttataa attttattcg aggaaaaaat atggcttttg cgtcattatt tacattatta
    16981 gacgatattg ctgccgtgct tgatgatgta tcgatcatga ctaaagtggc tgctaagaaa
    17041 acagcaggcg taatcggtga tgatcttgcc ttaaatgcta atcaagtcag tggaaagaat
    17101 gtggtgccag aacgagaact accgattgtt tgggctgtag cgaaaggctc attaatcaat
    17161 aaagtgatct tgattccact agcgttgctg ctatcggtct ttttaccgca ggctattatc
    17221 ccgctattaa tgataggcgg tgcatattta tgttttgaag gcgtagagaa attactccat
    17281 cgtctattac ataaaaacaa tcaagctcat tctgatgaaa ccgaaaccct ttctgaaaaa
    17341 gataaaatta aaggtgcaat tcgcaccgat tttattctat cagcagagat tatcattatt
    17401 gcgttaggtg ttttgcaaga ctcttcaacc ctaacccaaa ttttatctct ctccgctatc
    17461 ggggtcggaa ttaccctgtt tgtttatggc ttagttggat taattgtaaa actagatgat
    17521 atcggtttat ggttaatgca gaaaaaaagc acattctcac aagcggttgg aaaaggctta
    17581 ttatttgcaa tgccgtggtt tatgaaagga ctttctgtga ttggtactat tgcgatgttc
    17641 ttagtcggtg gtggtatttt tacccataat ttaccaatga ttcatcactt cgtagaacaa
    17701 ttcggtatct ctccccattt ttctactctg gttgattttg cagtgggtat tgtggttggt
    17761 attattgcat gcttaattat tttacctttg atgaaattat tttctaaaaa ggcgcactaa
    17821 tccccaaaag cgtagctttg gttaaagcta gctttttatc tcttgctata aaaaaatagc
    17881 aggtttcaag cctgctattt ttttaattat tataatgagt tagactcagt ggctgtttgc
    17941 ggtaaggcta atggctcatt aactttagac tgagtagcca tattcacctg tttgagattt
    18001 tcctcacgaa tacggtttgc cattaatgta tcccccatct gctctgccac gtgcagtgcc
    18061 ataatacgat catcagcaac acattcatca tgagccaata acaattcaaa ctgagacttc
    18121 gctttttggt attgcccatc tcgtgtataa atatagccaa gcgctctagc ataatcatcg
    18181 gtatatttaa cattcgcttt atctgcccgt tttgctaata cttttagtag cttactgtca
    18241 gaatcaattt gtaaacgggt taattgcaca aataaacctt gtaattgctc atcttcaaat
    18301 ttcttcaacg tttctaaagc aatttcttgt gcagattgat gatcgtcact atcaattaaa
    18361 cgcttgatga caccgacacg actatacacc gatttacgac gacgatttgg ttggttttcc
    18421 caccaagaaa gcaagccttc ctgcccttct tcctgtaaac gctcatcttg taaaccatca
    18481 tcaacgaaat actctaactc agcatactct tctgcagtta agaaattacg ttggccgatt
    18541 tcaactaata atctatctaa cgcaacataa gctctggatt ctttataaat ttgaatcgct
    18601 aagcgtaata cctcatcgct atgaggtgca agctctagta ggctatcaat cgcagttcgg
    18661 gcagccggta attttccttg ttgtaataaa attcgggttc gtaccaactc cactgcaaca
    18721 ttattcggtc cagctatttc agttgcctta attaaatatt tatttgcagc taaatcatca
    18781 ccattttgct gagcagcttc cgccgcttta attaaattca gtacaggctc atctgcatgt
    18841 tttgcatttt tactaaagag tttttccgct tttgagtaat tcccttcact catacgcatt
    18901 aaaccttcca gtgtttcttg ctgagctttt ttatgcttac gagttgagaa ccagttataa
    18961 gctccattac tcatacggca tattttagtg acaatccatt caagtaaata aactaccgcc
    19021 attgccaaca caaaaaaaac cacaagcatt acaatgctca tttcaatcac ggtagttgct
    19081 gtttcaattc tgacataacc ttgatttccg gctaaataag gcccagagat tagtccaact
    19141 aataataaaa gcatcaaaaa gagcgttcta aacataatct attctccttt ttattattgt
    19201 gctgaaagag gcatcgcatc ttgctctgct tttacaggct ctgcttgctc taattctttt
    19261 tcagctttta tttcgacttt ctctattttt tcaggggttt tattaagttg ctgagcaatc
    19321 acctttaagc tttgtaatga agttggagca tccacataaa tagtttgttc tgccaactca
    19381 tctagctctt tcaagaaatg ttgcacattt tcatttgaag tttcaaaata acttcttacc
    19441 caagagccta ccgtttgtaa cgatttttta taaagttcgc tttgctggcg aggaacagct
    19501 aaaatcgcaa tttgtaaacg caagcgaatg ttttcacgca aataaatttc ttgattcggt
    19561 gcaacaaagg ctttctcatc tgctgcatta cgtttcgata cacgaataaa atgatctaaa
    19621 aatgaagcag cacttttttc taaatttttc tcccaatccg caagagaatc acttacttgc
    19681 ccatcttgtt tgctatttga agcttgttca ctttccaaaa tcggcaaatc atctaattta
    19741 ttcgctaaat ttgcgacacg ctgcattaag gcattttgat cgatctgatg caatgcggtt
    19801 aatgtattta aatcagtttt gattgcctca cgaatgtgaa tcgtattcgg tgcttttgat
    19861 aaaacttgat ctgcctctaa caataaattt ttcgctgttt caatgtcatt atctaacacc
    19921 actttacgag ccgcattatt caataggaaa tctgcctcag ataatagcca gaatgtgtta
    19981 tccgaaccag aggtattatt tagcttttgc agttgaagct gcaaactact aatttgctgg
    20041 gtataaccat tttgggctgt ttctaaagct ttaatccgtt ctaaagcttt ttgatattcc
    20101 gtgaccaatt cggcaatttg tgctttttca ttttcgaaat ttggcatctc aaccatagtt
    20161 tgagtagtag gctgatttgc tttagcagat actgcctgaa tttgagattc aacctctgca
    20221 aactttttat ttgccatata atgaccagct ccgccaaccg ctaaagccac taatagtgca
    20281 actaatgcca agccctttcc gcccgatttt tgaacaacca cttgttcttt tggcgctttt
    20341 tgcatttctg tttgaatatc tttctctata gcttcgttca ctacatcttc ctcaatctct
    20401 ttttttgcaa aagatcccgc tgaatcgacc gcttgttgtg cctcttctac caccttttca
    20461 gctgaatgct ctaaatgttc agttactttt ttagatcttg acatactcgc cacctctagt
    20521 tagatttgcc cactttaaat aataatgtat tcatcaaagt tacattatcg gctttatccg
    20581 ataccctaat cttatcttct tgccagccca tttgtttagc tatctttgca atccgccggc
    20641 tcactacaac cagatcacat tcaaataacc atgctctaca atctgctttc gcttgttcat
    20701 aaagtgatct taaaatttca ctactggtga tcacaatagt atctactccc aagcgtttac
    20761 ataagctgat tttttcggga atatcatcgg taaccggctc tctacgataa cactccaaat
    20821 attgaatagt tgccccacgc aagactgcag tttcagcaag tagctcacgc ccagaatcgg
    20881 ctcttagaat taaaatagtt ttatcagtta gattctgcat ttcaggtaat gccaaaaccc
    20941 cctcgctatt ttcaaattct atcggataaa tgaccgcttg ttcagctttt tctgttaaat
    21001 atttcgcagt ccgttgccct accgcaaaat atttcaaatc tgaacgaaaa tggaacccgg
    21061 tatctactaa tgtttttgca gcatagtcca ctgcattcgg cgaaacaaca aatacataat
    21121 cgtctgattt tagccgagaa atagccgaag gaagcaaagg taattcttta ccggcttcaa
    21181 tcgtaaatag cggttgatga atagcaaaaa tttgctgttc attcaacata tctaccaatt
    21241 cttgcccccg actatccagg cgtgtgacca acacattcat tattgtacgt tttcgttata
    21301 aaccgcagct aaaattttat ctgcaccttg agcaagcaac tgttccgcca ccaaaatgcc
    21361 taattgctcc gcctcttgct tatgtccttt tgccgaagcc cgaataattt gtgagccatc
    21421 taacgatcca actaacgcat taagcgttaa ttcatcgccc tctaatgtag cataaccacc
    21481 aatcggcact tggcaaccgc cttgcaaacg ggtattcatt gcacgttctg ccatcacgca
    21541 acaagcggtc gaactatggt ttaattttgc aatgtagttc aaaatccgtt catcattggc
    21601 tcgagtctca atccctactg ctccttgacc agctgcgggt aatgattgtt ccacagaaat
    21661 atagctacgg attcgctctt gcaagcctaa acgaattaag ccggcagagg ctaaaataat
    21721 cgcatcatac tcgccattat ctaatttgct taagcgagtg ccgacattgc ctcgcaatga
    21781 tttaacctct aaatgcggat atttcgccat taattggcac tgacggcgta agctcgatgt
    21841 accgaccaca gcacctttcg gcaaatcttc taacgaatgg tagttgttcg acacaaacgc
    21901 atcacgggga tcttcacgtt cacaaatcac cgcaagcccc aagccttcgg gaaagctcat
    21961 cggcacatct ttcattgaat gcaccgcaat atcagctcga ttttctaaaa gggctaattc
    22021 taactctttc acaaacaagc cttttccgcc aattttggct aatggggtgt ctaaaataat
    22081 atcgcctttc gttaccatcg taaccagttc gacagataat tgcggaaaat gtttttctaa
    22141 ttcgcttttt acaaaatttg cctgccataa ggctaacggg ctttgacggg ttgcaatgcg
    22201 taaaatatct ttcataaatt ctaaaattaa aacattgagt taatgaatta tcctaccact
    22261 ttttagctga actgccaaaa ataatcgtgg atttcgcttt gcatttaatc aaaaatccga
    22321 ctttttctca aaatgtatca
//
LOCUS       DANLXS010000017        21351 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.017, whole genome shotgun sequence.
ACCESSION   DANLXS010000017 DANLXS010000000
VERSION     DANLXS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21351
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.017"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..289)
                     /locus_tag="PXD71_000536"
     CDS             complement(<1..289)
                     /locus_tag="PXD71_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL6290693.1"
                     /translation="MKSLVIKLFGTKKEKKTFKFSKIESVLLKPIGDAIGDAVVHIAH
                     LEQIKKALPNVKIAVLVTSRNKLIFSQANVVDMILEDKVSTYLSQRGHWDLY"
     gene            337..1368
                     /locus_tag="PXD71_000537"
     CDS             337..1368
                     /locus_tag="PXD71_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL6290694.1"
                     /translation="MNIKATLRKARLVIGKAILDSKNTDLILSEIPNKILFLRHDGKI
                     GDYVVSSFVFREIKKQSPQTQIGVVCSQKNRYLFENNPYIDKLYLVKTKSIPDYIRGG
                     KQLAKEQYDVVIDPTVTLRNRDLLFLRTISAKHYVGYQKQKYRLFDLNVEDRKQHFSE
                     IYQDALALIGFENIETQYDVPLNATSNQSIKHYLYENQIRNYIALNLFGAGSARRFSE
                     QKINELLSCLKQHSDKPIVLLTFPEVTAKLCSFTKKYKNIFVYEKTKSVFDSIELIRF
                     ADLLISPDTATVHIASGLSKKIIAFYSSDEQNFIHWHPNNQAETHILRFRKSVNDLDF
                     AAIKPEWLK"
     gene            1370..2260
                     /gene="ilvY"
                     /locus_tag="PXD71_000538"
     CDS             1370..2260
                     /gene="ilvY"
                     /locus_tag="PXD71_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDL6290695.1"
                     /translation="MDFQSLKLFLDIAQSRSFIRSAEKNYMTASTLTRHIQRIEEELG
                     QPLFLRDNRQVRLTEAGERFLAFAQQGWAEWQQLQNQLSPAQGDLEGELKVFCSVTAS
                     YSHLPPILGKFRQKYPKVDIKLSTGDPALALEQVQSLAADISVAGKPEFLPNNIVFHY
                     IDDIQLSIIAPRVASQATQFLQQSPIDWKNIPFILPVNGPVRKRIDRWFKEQKIKDPI
                     IYATVSGHEAIVPMVALGFGVALLPDIVIKHSPMNNQVSYVNLPSPITPFELGICVQQ
                     KRLQEPIINAFWQLLSDNSA"
     gene            complement(2394..4796)
                     /gene="lon"
                     /locus_tag="PXD71_000539"
     CDS             complement(2394..4796)
                     /gene="lon"
                     /locus_tag="PXD71_000539"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDL6290696.1"
                     /translation="MAPRKKKAIELPLLPLRDVVVFPYMVMPLFVGREKSIQALRSAM
                     DSNKQLFLVTQQDPNKEEPNAEDMYGVGVIANIIQMLNLPDGTVKVLVEGQTRAKIEQ
                     IHDDENGFWAAIQPIYSEYDDENEELKAIAKTTLTEFENYVKNNKKIPAEIIAKLQKI
                     TLEDRLADTIASNLIAPVKKKQELLEQPNLIARFEALLIAMATEMDTLETETRIRNRV
                     KQQMEKNQRDYYLNEQIKAIQKELGNGDDVEQSELDKLKEKIDAAKLPTEVKDKLNTE
                     FKKLKAMPQSSSEATVVRGYIDWILQMPWHKKSAVKKDLAQAQEILDKDHYGLERVKE
                     RIVEYLAVQSRLNKLKGPILCLVGPPGVGKTSLGQSIANATGRKYVRMALGGVRDEAE
                     IRGHRRTYIGSMPGSLMMKMAKVGVRNPLFLLDEIDKMAQDMRGDPASALLEVLDPEQ
                     NKAFNDHYLEVDYDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKMHIARGHL
                     LAKQQENNGLKEGELTIEDSAILSIIRYYTREAGVRSLEREIAKICRKAVKALVLDKK
                     LKSITVSEKNIEEYLGVKRFDYGKMDSQNRIGEVTGLAWTEVGGDLLTIETASVAGKG
                     KFSYTGSLGDVMKESIQAAMVVVRSRAEKLGIAEDFHEKRDIHVHVPDGATPKDGPSA
                     GIAMCTALISSLTGNPVRKEVAMTGEISLRGKVLPIGGLKEKLLAAHRGGITTVIIPK
                     ENEKDLEEIPENAKAALDIHAVETIDEVLALALENPPMGIEILSKAEIKVKKSRAKAT
                     VQ"
     regulatory      5000..5118
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5294..6391
                     /gene="ribD"
                     /locus_tag="PXD71_000540"
     CDS             5294..6391
                     /gene="ribD"
                     /locus_tag="PXD71_000540"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDL6290697.1"
                     /translation="MTDVQYMAYAITLAEKARGWTSPNPLVGCVIVKNGNIIAEGYHQ
                     KAGSWHAERNAILNCEEDLTGATAYVTLEPCCHHGRTPPCSELLIERGIKKVFIGSRD
                     PNPLVSGKGAAQLRAAGIEVVEDFMRTECDALNPIFFHYIQTKRPYVLLKYGMTADGK
                     IATYTGNSKWITGEAARANVQKSRHEYSAIMVGVETVLADDPMLNSRMENAKQPVRIV
                     CDSQLRTPLDSQLVQTARQYRTIIATLNNDAKKQAEYTACGVEILVTGERDKRVDLAD
                     LLQKLGELGIDSLLIEGGSQLNFSVLEQGLVNRVHCYIAPKLIGGKAAKTPIGGKGIG
                     ELAQAVKLRLKSTELLGEDILLDYEVDLLRN"
     gene            6608..7255
                     /gene="ribE"
                     /locus_tag="PXD71_000541"
     CDS             6608..7255
                     /gene="ribE"
                     /locus_tag="PXD71_000541"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDL6290698.1"
                     /translation="MFTGIIEEVGQIAQIKKQGEFVVVTVKAKKILTDVHLGDSIAVN
                     GVCLTVTSFTQEQFTADVMSETLKRTSLGELSINSSVNLERAMAANGRFGGHIVSGHI
                     DGTGVVAEITPADNSTWYRIKAEPKLMRYIIEKGSITIDGISLTVVDVDAESFRVSII
                     PHTIKETDLGTKKIGSLVNLENDIVGKYIEQFLLKKEPENPQSKITADFLKNAGF"
     gene            7274..8473
                     /locus_tag="PXD71_000542"
     CDS             7274..8473
                     /locus_tag="PXD71_000542"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDL6290699.1"
                     /translation="MFKFSTVEEAIQAIAQGKIILVSDDADRENEGDFICAAEFATPE
                     NINFMAKYGKGLICTPISTEIAKKLNFHPMVLENEDNHQTAFTVSVDHIQTGTGISAF
                     ERSLTCLKMLDENAKPEDFRRPGHVFPLVAKDGGVLVRNGHTEATVDLARLAGLKPAG
                     LCCEIMAEDGTMMQMPELQAFAQAHNMPFITIQQLQEYRRKHDSLVNVVSVVKMPTKY
                     GEFKAHSFVETISGKEHVALVKGDIGDGENVLCRIHSECLTGDAFGSQRCDCGQQFAA
                     AMNQVEQEGRGVVLYLRQEGRGIGLINKLRAYELQDRGMDTVEANLALGFKDDEREYY
                     IAAQMFEKLGVKSIRLLTNNPAKIEGLIEQGLNVVAREPIMVEPNEHDLEYLKVKQHK
                     MRHMFNF"
     gene            8679..9143
                     /locus_tag="PXD71_000543"
     CDS             8679..9143
                     /locus_tag="PXD71_000543"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDL6290700.1"
                     /translation="MATFQGNYIATGLKFGIVATNWHKIFIDQLLNGATDKLLRHGVE
                     EKNIDTVWVPGALEISIAAKKMAQSGRYDAIICLGAVVRGATSHYDVVVNESAKGISS
                     SALETGVPIINGILTVENLEQAIERSGTKAGNKGEECAMVAIEMANLLKALA"
     gene            complement(9189..10697)
                     /gene="lnt"
                     /locus_tag="PXD71_000544"
     CDS             complement(9189..10697)
                     /gene="lnt"
                     /locus_tag="PXD71_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDL6290701.1"
                     /translation="MNFAKNPPFLTACLLAIILGAIGTFAYSPFDIWILAFASAAGLV
                     WSATLANRKTALWATFAWSIGYFCTGVNWVSVSMTQFGGVPTIVSYIAVFLLACYLAI
                     YNLLFSVVSHRLKLNNPFALAAIFTFTEYLRGVVFTGFPWLQFGYSLIDSPFAGIAPI
                     LGVEGLTFLVIVASSYLVKIIQQENKTVPSLMVLAVIFGLSFATRFLQFVQIDTEKQP
                     LVVSLVQGNIEQKMKWDPQHFNHSVRTYEQLILPLLGNSELIILPESAIPALENQISP
                     LLGSLDHLARQHGSEIIIGTLYQNEQEELFNSSVLLGEKEREYALHSNPRYNKHHLVP
                     FGEYVPFGSVLDWMREVFILPINLSQGSFVQPSFLAKNNRFNMAICYEIIFGDQLQQN
                     QKSQNSDYLLTITNDAWFGSSIGPWQHFQMARMRALELGKPLLRAANTGITAIVDANG
                     KVIEQLPQFTADALTATIVPTKGDTPFKQLGHWLIYSFSLFCVIASLLLRRK"
     gene            complement(10756..11655)
                     /gene="corC"
                     /locus_tag="PXD71_000545"
     CDS             complement(10756..11655)
                     /gene="corC"
                     /locus_tag="PXD71_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249295.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDL6290702.1"
                     /translation="MSDEQQSVNTTEKSEKKSILHSLFSGLFQNEPKNREDLVEVIRD
                     SVENELIDSDTKEMIEGVMEISELRVRDIMIPRAQIVFIDANEPLDVCIDLIIESAHS
                     RFPVIRGEKDTIEGILHSKDLLKYLRSDSEPFNLQQVLRPAVIVPESKRLDRMLKEFR
                     SERFHMAVVVDEFGAVSGLVTIEDILEQIVGDIEDEFDEEEVKPIRQLSRHTYAVLPL
                     TDIKKFNEFFATDFNDEEVDTIGGLVMQAFGYFPKRDEQIELNGIDFKVTSANSRQLI
                     QLRVTLSDEQLEKMEKTSPNEEE"
     gene            11793..12401
                     /locus_tag="PXD71_000546"
     CDS             11793..12401
                     /locus_tag="PXD71_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDL6290703.1"
                     /translation="MKLTKIALSLTALVVSSMSVAGTLSTSSMVDILAFDGQRVKSGT
                     TSVQMSENKTHQVVVEVGGTVEGDFFTSDQIILTFQGSAENAKLETPRLNSKLDLRKF
                     QENQTVAIKTDSGKVISHKQDFLKGEGFLAKTRVEDNLTKYNLAKKTASVEQFATAPL
                     EAKGQIVVDTNKVSQEELQLLFRKADKDTQKRFLEWAKKNIK"
     gene            complement(12482..12865)
                     /locus_tag="PXD71_000547"
     CDS             complement(12482..12865)
                     /locus_tag="PXD71_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290704.1"
                     /translation="MKTLKLGCIFSLALMLSSCTLTPEQQAERKAKQIRAEQDLQVQL
                     AKQCDVETAELMYQQFNPPLSQTEQEEKAFKKRYAEKVNAPMFQACYKLAWQNYKAQV
                     ELEEIRWNYDRDYMYRGWRYCYYCW"
     gene            complement(12895..13464)
                     /locus_tag="PXD71_000548"
     CDS             complement(12895..13464)
                     /locus_tag="PXD71_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDL6290705.1"
                     /translation="MNETTINDFSLLCRLFGNLFYRHPTDPILAGTFNWLAQGGLRQV
                     WALNTDSQSENALTLLQKNANPTQLAASYQALFAENGAISPTISAYGLSVNNFVAFRQ
                     ERAMPELENADHIGLLFLTASWIEDNLDSALAQKILFEQFLLPCAGKFLGKIEAFDNG
                     FYKALAQLSRDALSAMADELEESELEAVE"
     gene            13523..14368
                     /gene="nfo"
                     /locus_tag="PXD71_000549"
     CDS             13523..14368
                     /gene="nfo"
                     /locus_tag="PXD71_000549"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL6290706.1"
                     /translation="MKYIGAHVSASGGVENAVLRSVEIGANAFALFTKNQRQWQAPAL
                     KAETIEKFKRFCKVHHFSAEQILPHDSYLINLGNPEAEALEKSRVAFIDEMSRANQLG
                     LKLLNFHPGAHLKQISEQDCLARIAESINIAVEKVPNVIAVIENTAGQGSNLGWHFDH
                     LATIIEQVEDKSRVGVCLDTCHLFSAGYDISSYEKSEAVFNDFNQTVGFHYLRGMHLN
                     GSKTPLGSRVDRHHTLREGTIGTEVFAFIMQNMHFNHIPMILETISPEIWADEIKFLR
                     TLELK"
     gene            14496..15068
                     /locus_tag="PXD71_000550"
     CDS             14496..15068
                     /locus_tag="PXD71_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6290707.1"
                     /translation="MKKNLFATTFKPVFIASILSVFGGYSSIAVASLSSKPATPNQII
                     ASKAERLAKSSSVFTNRANLPTSSAHTANYSGRINSVYHNWVGTRYRLGGTTKAGIDC
                     SAFVREVMGKAFSKDMPRSTAEQRHVGHSISKSDLRPGDLVFFRKNNHVGVYIGNGKF
                     VHASSSRGVTTSSLSESYWSRNYTQSRRVI"
     gene            complement(15135..15593)
                     /locus_tag="PXD71_000551"
     CDS             complement(15135..15593)
                     /locus_tag="PXD71_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6290708.1"
                     /translation="MASIRQISDQTAAYQPIPLYRDFNSSRSVKLDLNSLCIKRPRDT
                     FFIHVKNPNLIAWGIELDDLLIVEQTNQYLVNDLLVLEKDGEYKFYQFFNEIQNGEGI
                     AEKILFSLDVSESNLRITDWAEVKIAGVITNVVHQMRTRGAFTQAKQRVA"
     gene            complement(15767..16150)
                     /gene="grcA"
                     /locus_tag="PXD71_000552"
     CDS             complement(15767..16150)
                     /gene="grcA"
                     /locus_tag="PXD71_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDL6290709.1"
                     /translation="MIKGVQITESSNSNLLNSFWLLDEAKNEARCLAAKAGFEEDQVV
                     AISELGDIAYREVPVNVAPTIKVEGGQHLNVNVLRRETLEDAIQNPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVITRTFTESL"
     gene            16309..16998
                     /gene="ung"
                     /locus_tag="PXD71_000553"
     CDS             16309..16998
                     /gene="ung"
                     /locus_tag="PXD71_000553"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL6290710.1"
                     /translation="MKTWTEALGEEKQKAYFQQILQYVHNERLAGKVIYPPQNEVFSA
                     FALTEFSDVKVVILGQDPYHGPNQAHGLAFSVKPPVAPPPSLVNMYKELSEDIEGFQI
                     PPHGYLIDWAKQGVFMLNTSLTVEQGRAGSHSQIGWEIFTDKVIAQLNQHRDNLVFLL
                     WGSHAQRKGQFIDRNRHCVLASPHPSPLSAYRGFFGCKHFSKANNYLISQGISPINWQ
                     LSVDVSYSSTH"
     gene            17014..17934
                     /locus_tag="PXD71_000554"
     CDS             17014..17934
                     /locus_tag="PXD71_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDL6290711.1"
                     /translation="MNFDLPIVSIAFVVLVLVALSSTIKIVPQGYHWTVERFGRYTKT
                     LSPGLNIVVPFIDRIGRKMNMMEQVLDIPSQEVISRDNASVAIDAVCFVQTVDARRAA
                     YEVNHLEQAIVNLTMTNMRTVLGSMDLDDMLSQRDLINGRLLSIVDEATNIWGVKVTR
                     IEIRDVRPPKELVAAMNAQMKAERNKRADILEAEGIRQAEILRAEGEKQSRILKAEGE
                     RQEAFLQAEARERAAEAEAKATQMVSEAIAKGDTTAINYFIAQKYTEALKEIGSSDNS
                     KVVLMPLEAGNLIGSVAGIAEILKSNKTSS"
     gene            17970..18407
                     /locus_tag="PXD71_000555"
     CDS             17970..18407
                     /locus_tag="PXD71_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL6290712.1"
                     /translation="MDWFFNWIGWSILGFVLLALELVLPGVFIMWWGFASLILAFIVA
                     LFTLGQALQIALFAIIATIFSLIWWKYQHGKDKKEDQSSSLNSREHAMLGAQGFVVEI
                     LPNGIARGKFGDTTWRVIGNNLQVADKIEVQKVEGITLFVRKQ"
     gene            complement(18548..21343)
                     /locus_tag="PXD71_000556"
     CDS             complement(18548..21343)
                     /locus_tag="PXD71_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL6290713.1"
                     /translation="MKHSFNKTLIAISISSFLSIAYATESIENPQPIIQLSESLSSKY
                     SGKGVKLGVMDEGFMVKHPRHSSHLHPLIHQLTTPEGEVRIYDASYPQFEVNPVEKED
                     GIDLIPSLETHGAGVAGIIAAQADKTLGDGYSGGIAKGAELYVATKSYKRTLEKVIQD
                     AKKELENAKDEEDEKTPSLDQMAKNDLLASKEKEMAIERAEWASGLNKLLDNNVFAIN
                     NSWNPFSISDDINVVDKFYQSIKQNKHNPLLQAIMRAKNSNTLLVFAAGNESKKQPGV
                     MALLPRYFPELEKNLISAVAVDKEQKIASYSNHCGASKNWCVAAPGDLHVLIGVADEH
                     KKPQYGLTKEQGTSFSAPAITASLAVLKERFDYLTATQIRDTLLTTATDLGEKGVDNV
                     YGWGLINLKKAVNGPTQFLNDETITVTRDDHWSNPLASQFKITKKGDKSLHLDGENHL
                     DTVAVEEGRLALNGKTKVKTISNHANLAVNGTEVEQNYSSSGQSQLEVLGKSGLIANA
                     QANIHLAGSLKIDDKLTEKTEAGDVSATVVQLKDKATYQGGFTQLVENENLAKRGLIQ
                     DLYFKESEIIAKVNKPLTDEKADTNGQAGLALLNALRTTPIAYRRSWYNGWLQSALEQ
                     RKLDNLHYAVSNNIYADSLELLRSQNAKGLTQAQQHLFTAYHTPLQTTVWAEHLNQKQ
                     SASSKHTDVKHHQSQLGVNHKLADKTVLSATLSQQKNRLEKPFAQATLKQTALNIGLR
                     YHLDNAWFSEATLQFARQKYQQSRRFASHQLGTAETRGSTLGGEMRIGYQFMPNQWII
                     EPSLGVQWIQTKMNGLNESGELATQTAAMRYRDVNIVPSVKLQRTFQLEQGSISPYIG
                     LNYLHRLNGKITKITSNIAGKTLHSEATTKRNRQLNGEVGVKLHYKNWFTAMNLDYSR
                     VKSANLFGWKVNVGFSF"
BASE COUNT         6273 a         4094 c         4439 g         6545 t
ORIGIN      
        1 aataaagatc ccaatgtccc cgctgcgaga gataagtgga tactttatct tctaagatca
       61 tatctaccac gtttgcttga gagaaaatca atttgttacg agatgtaact aaaaccgcta
      121 tttttacatt tgggagagct tttttaattt gctccaaatg agctatatgc acaacagcat
      181 cgccaatagc atctccgata ggtttgagta atacggattc tattttgcta aatttgaagg
      241 tttttttctc tttttttgta ccaaaaagtt ttataactaa ggatttcatc tctataatag
      301 gttgattctt ttaatgattg gttatagtaa gtaaaaatga atattaaagc aacgttaaga
      361 aaagcaagat tggtgatagg taaagccatt ttagatagta aaaatactga tctgatatta
      421 tcggaaattc ccaataaaat tctatttctt cgtcacgatg gtaaaattgg tgattatgtc
      481 gttagttcct ttgtcttccg tgaaataaaa aaacagtctc cccaaacgca aataggcgtg
      541 gtttgctcac agaaaaaccg atatttattt gagaataatc cttatattga taagctctat
      601 ttggttaaga caaaaagtat tcctgattat attcgtggtg gcaaacaact tgcaaaagag
      661 cagtatgatg ttgtaatcga tccgactgtt actttacgga atcgtgatct cttattttta
      721 cgcactatat cagccaaaca ttatgtaggt tatcagaaac aaaaatatcg attatttgat
      781 ctaaatgtag aggatagaaa acagcatttt tcagagattt atcaagatgc gttagcttta
      841 atcgggtttg aaaatataga aactcagtat gatgtgccac taaatgccac aagcaaccaa
      901 tccataaagc attatttata tgaaaaccaa attagaaatt atattgcgtt aaatcttttt
      961 ggtgcaggtt cggcaagacg ttttagtgag cagaaaatta atgaactgct gagttgctta
     1021 aaacagcata gtgataaacc gatagtctta cttacttttc ctgaagtaac ggcaaaattg
     1081 tgttccttta ctaagaagta taaaaatata tttgtctatg agaaaaccaa aagcgttttt
     1141 gattcaatag aactgattcg ttttgcggac ttattaattt cacctgatac tgcaaccgtg
     1201 catattgcct caggcttgag taaaaagata attgcttttt acagtagcga tgaacagaat
     1261 tttatacatt ggcatccgaa taatcaggct gaaacccata ttctccgttt tagaaaaagc
     1321 gtgaatgatt tagattttgc tgcaataaaa ccggagtggc taaaataata tggacttcca
     1381 aagccttaaa cttttcctag atatagccca aagccgtagt tttattcgca gtgcggagaa
     1441 gaattatatg acggcttcca cacttacccg ccatattcag cgtatagagg aggagctcgg
     1501 gcagccgttg tttctgcgtg ataatcgaca agtgcgttta actgaagcag gggaaaggtt
     1561 tttggcgttt gctcaacaag gttgggcaga gtggcagcag ttgcaaaacc agttgtcgcc
     1621 tgctcaaggt gatttagaag gagagttgaa agtgttctgc tcggtaacgg cttcttacag
     1681 ccatttaccg ccgattttgg gaaagtttcg ccaaaaatat ccgaaagtgg atattaaact
     1741 gagtaccggt gatccggctt tggcattgga acaggttcag tcattggctg cagatatttc
     1801 ggttgcgggt aagccggaat ttctgccgaa taatattgtg tttcactata ttgatgatat
     1861 tcaactttcg attattgccc ctcgtgtggc gagccaagcc actcaatttc tgcaacagtc
     1921 gccgatagat tggaaaaata tcccctttat tttgccggtg aacggacctg tacgaaaacg
     1981 gattgaccgt tggtttaaag agcaaaaaat caaagacccg attatttatg ctaccgtctc
     2041 cggacacgaa gcgattgtgc cgatggttgc actaggcttt ggggtcgcac tgctaccgga
     2101 tattgtgatt aagcatagcc caatgaataa tcaagtttct tatgtgaatt taccctcgcc
     2161 gatcactccg tttgagctgg gaatttgcgt tcagcaaaaa cgcttgcaag agccgatcat
     2221 caacgcattt tggcaattat tgtctgataa ttcagcataa caagcggtta gattttgcta
     2281 aatttttgca aaaaatagag caaaaccgac cgcttgtaga taaaactttc agataaaaaa
     2341 taaggtgcgt gattgtgatc tgcgcacctt atgttgtttt agattattgc actttattgc
     2401 actgtggctt tagctcgcga ttttttcacc ttgatctcgg cttttgacaa gatttcaatc
     2461 cccattggcg ggttttctag tgccagagct agtacctcgt caatggtttc tactgcgtga
     2521 atgtccaacg ctgcttttgc attttccgga atttcctcta agtctttttc attttctttc
     2581 ggaataatca ccgtcgtaat accaccacga tgggctgcca gtaatttctc ttttaagccg
     2641 ccgattggca atactttgcc acgcaagctg atttcaccgg tcatcgctac ttctttacgc
     2701 acagggttac ctgttaggct ggaaattaat gcagtacaca ttgcaatccc tgcactcgga
     2761 ccatctttcg gtgttgcacc gtccggcacg tgaacgtgaa tatcccgttt ttcgtggaag
     2821 tcttcagcaa tgcctaattt ttctgcacga gagcgtacaa ccaccattgc cgcttgaata
     2881 gactctttca ttacatcgcc taatgaaccg gtgtaagaga atttgccttt acctgcaaca
     2941 gaagcggttt caatggttaa taaatcaccg ccaacttctg tccacgctaa accggtcact
     3001 tcgccaatgc ggttttggct atccattttg ccgtagtcaa aacgttttac gcctaagtat
     3061 tcttcgatat ttttctcaga aactgtaatg gattttaatt tcttatccaa tactaaagct
     3121 ttcacggctt tacggcagat tttcgcaatt tcacgctcaa ggctacgcac gcctgcttca
     3181 cgggtgtagt agcggataat acttaaaatt gcactgtctt caatggtcag ctcaccctct
     3241 tttagtccgt tattttcttg ctgtttagcc agtaaatgac cacgagcaat gtgcattttc
     3301 tcatcttcgg tatagcctga aagacgaatc acttccatac ggtcgagcaa tgctggcgga
     3361 atgttcattg agtttgaggt tgccacaaac attacatcgg ataaatcgta atccacctct
     3421 aaataatggt cgttaaaggc tttattttgc tcaggatcta acacttcaag cagagcagaa
     3481 gccgggtcgc cacgcatatc ttgagccatt ttgtcgattt catctaacaa gaacagcgga
     3541 ttacgcacgc cgactttcgc cattttcatc attaggctac ccggcattga gccgatgtaa
     3601 gtacggcgat gcccacggat ttccgcttca tcacgcacgc cacctaacgc catacgcaca
     3661 tatttacgcc ctgtggcatt ggcaatagat tgccctaatg aggttttacc aacccccggc
     3721 ggaccaacta agcacaaaat cggacctttt aatttattta gacgactttg cactgctaag
     3781 tattctacaa tccgctcttt cactcgttct aagccgtagt ggtctttatc caaaatctct
     3841 tgagcttggg ctaaatcttt tttgaccgct gattttttat gccacggcat ttgtaaaatc
     3901 cagtcaatat aaccacgcac aacggtagct tcggaagagc tttgcggcat tgctttgagc
     3961 tttttgaact ccgtattcag cttgtctttt acctcggtcg gtaattttgc agcgtcaatt
     4021 ttctctttaa gcttatctaa ctcgctttgt tcaacgtcat cgccattacc taactctttt
     4081 tgaatcgctt taatttgctc gttgaggtag taatcacgtt ggtttttttc catttgctgt
     4141 ttaacacgat tacgaatccg agtttcggtt tcaagcgtat ccatctcagt tgccatcgca
     4201 attaacaagg cttcaaaacg ggcaatcaga tttggttgct ccaataattc ctgttttttc
     4261 ttcaccggag caattaagtt agaggcaatg gtatccgcca agcggtcttc caacgtgatt
     4321 ttttgcagtt tagcaataat ttccgccgga attttcttgt tatttttaac gtaattttca
     4381 aattcggtta aagtggtttt agcaatcgct tttaactctt cgttttcgtc atcatattcc
     4441 gaatatattg gctgaattgc cgcccagaag ccgttttcat catcgtgaat ttgctcgatt
     4501 ttggcacggg tttgtccttc taccagcact ttaactgtgc cgtccggcag gtttaacatt
     4561 tgaataatgt tggcaattac acctacgcca tacatatctt ctgcattcgg ctcttcttta
     4621 tttggatctt gctgagtgac taaaaacagt tgtttattgg aatccatcgc cgagcgtagg
     4681 gcttgaattg atttttcacg tcctacaaat aacggcataa ccatataagg aaaaaccacc
     4741 acatcacgca atgggagtag tggtaattca atcgctttct tttttcttgg tgccataaat
     4801 ttgctctctc tatcaaaata taatccccca ttatgagggc gaatttggga aaaacaaggg
     4861 aaaatttgat ttatcttaaa aaaactcgaa tagtcaaaac agtagatgtt ggcgatttaa
     4921 tcaattgtta aaatcagggt aattagctta atttcccgct tgacatcaaa tcgtaatgcg
     4981 agcaaaatcc cctgcatatt ttaatcttca gggcagggtg aaattcccga tcggcggtaa
     5041 agtccgcgag ccgagcaatc ggcaggattt ggtgaaattc caaaaccgac agtatagtct
     5101 ggatgaaaga agagtcaaaa accgcaaaaa ataccgcaca agcggtcatt ttttgtgtct
     5161 tttttgcaag ccttgagtcg tgagatttca aggcttcatt gttttgcagg cggcgtttgc
     5221 aaatttttta ctaaaaacga ccgcttgtat tgcaataatc ctttcattac gccctcaatt
     5281 tatcaaaatg tatatgacag atgttcaata tatggcttat gccatcaccc ttgccgaaaa
     5341 agcacggggt tggacaagcc ctaatccgct agttggctgt gtgatcgtga aaaacggcaa
     5401 cattatcgcc gaagggtatc accaaaaagc cggtagttgg cacgctgaac gcaatgcgat
     5461 tttaaactgc gaggaagatc tgacgggggc aaccgcttat gtaaccttag agccttgttg
     5521 ccaccacggg cgaaccccgc cttgttccga actgctgatt gaacgtggga tcaaaaaagt
     5581 gtttatcggc tctcgagatc cgaacccact tgtttctggc aaaggagcgg cacagcttag
     5641 agccgccggc attgaggttg tggaagactt tatgcgaacg gagtgcgatg cgctgaaccc
     5701 gattttcttc cattacattc aaaccaaacg cccttatgtg ttgctgaaat atgggatgac
     5761 ggcagatggt aaaattgcga cttatacggg gaattcgaaa tggatcaccg gcgaggctgc
     5821 ccgagctaat gtacagaaaa gccgccacga atatagtgcg attatggtcg gggtggaaac
     5881 ggtgcttgcc gatgacccaa tgctcaacag ccgaatggaa aatgctaaac agccggtgcg
     5941 gattgtatgt gacagccaac tgcgtacccc attggatagc caactagtcc aaactgcacg
     6001 ccaatatcgc accattattg ctacgctcaa taatgatgca aaaaagcagg cagaatatac
     6061 cgcttgcggt gttgaaattt tagtgacagg cgaacgtgac aagcgggtgg atttagccga
     6121 tcttttgcaa aagctgggcg aactcgggat cgacagctta ttgattgaag gtggttcgca
     6181 actcaatttc agcgtgctgg aacaggggtt agtcaatcga gtacattgct atattgcccc
     6241 gaaactgatt ggcggcaaag cggcaaaaac cccgattggt ggcaagggga ttggcgagtt
     6301 agcacaagcg gttaaattac gtctaaaatc caccgaattg ctcggagaag atattttgtt
     6361 ggattatgaa gtggacttgc ttcgtaatta aattgaaatt tccttactct cgcccgttta
     6421 cgggagagag acagcataaa atttcgttca gaaattttat gcagagagag gggaaaatga
     6481 gtgtaatctt ccctctccct ccgaaaatcc tacggatttt ctcttccctc tcccgtaaac
     6541 gggagagggg aatagtaaga taagcagttc tcagactaaa ttggaagtaa atcggagaaa
     6601 aatttaaatg ttcacaggca ttatcgaaga agttggccag atagcccaaa ttaagaaaca
     6661 aggcgagttt gtggtggtaa cggttaaagc aaaaaagatt ctgaccgatg tacatttagg
     6721 ggatagcatt gcggtaaatg gcgtgtgttt aaccgtgact tcatttacgc aggagcaatt
     6781 tactgctgat gttatgtcag aaaccttaaa acgtacatca cttggcgaat taagtatcaa
     6841 cagctcggtt aatcttgagc gggcaatggc ggcaaacggg cgttttggtg ggcatattgt
     6901 gtcaggtcat attgatggca cgggtgtggt tgcagagatt acaccagcag ataattcaac
     6961 ttggtatcgc attaaagccg aacctaagtt aatgcgttac atcattgaaa aaggctcaat
     7021 tactattgat ggcattagtt taacggtggt ggatgttgat gcggaatctt tccgggtatc
     7081 gattattccg cataccatta aggaaaccga cctcggtacg aaaaaaatcg gtagtttggt
     7141 gaatttagaa aatgacattg tcggcaaata tatcgaacag tttttattga aaaaagagcc
     7201 tgaaaatccg caaagtaaaa ttactgcgga ctttcttaaa aatgcagggt tttaaccaaa
     7261 taaaggacaa ataatgttta agttttcaac ggtagaagaa gcaattcaag caattgcaca
     7321 agggaaaatt attttagtca gcgatgatgc cgaccgtgaa aatgaagggg acttcatttg
     7381 tgcagcggaa tttgcgacac cggaaaatat caactttatg gcgaaatatg gtaaagggtt
     7441 gatttgtacg ccgatttcga cagaaattgc caaaaaatta aattttcacc cgatggtgct
     7501 agagaacgaa gataatcatc aaaccgcatt taccgtttct gttgaccata ttcaaaccgg
     7561 caccggtatt tccgcttttg agcgttcatt aacttgcctg aaaatgcttg atgaaaatgc
     7621 taagccggaa gattttagac gtccggggca tgttttccca ttagtggcaa aagacggagg
     7681 cgtattagtg cgtaacggtc ataccgaagc aactgtggat ttagctcgtt tggcgggctt
     7741 gaaaccggca gggctttgct gtgaaattat ggcagaagac ggcacgatga tgcagatgcc
     7801 ggaattacag gcatttgcac aagcacacaa tatgccgttt attactattc agcagttaca
     7861 agagtatcgt agaaaacacg acagcttggt gaatgtggtt tcggtggtaa aaatgccgac
     7921 caaatacggt gagttcaaag cacacagttt cgttgaaact atttcaggca aagaacacgt
     7981 cgctttagtt aaaggtgata ttggtgatgg cgaaaatgta ttatgccgta tccattccga
     8041 atgtttaacc ggcgatgcct ttggctcaca acgttgcgat tgtggtcagc agttcgcagc
     8101 ggcaatgaac caagtggagc aagagggcag aggcgtggtg ctttatttac gccaagaagg
     8161 acgtggcatc ggtttaatta ataaactgcg tgcttacgaa ttgcaagata gaggaatgga
     8221 cacggttgaa gccaacctgg cattaggttt caaagatgat gagcgtgaat attatatcgc
     8281 cgctcaaatg tttgaaaaat taggcgtaaa atcgattcgt ttgttaacca ataatccggc
     8341 aaaaattgaa ggactcattg agcaaggttt aaacgtagtg gctcgtgagc cgattatggt
     8401 tgagccaaac gaacacgatt tggaatacct taaagttaaa cagcacaaaa tgcgacatat
     8461 gtttaatttc taatccaata ctctctcccc ttgtgggaga gagacagaaa aatacttcgt
     8521 tcagaagtat ttttcagaga gagggtatat tttttagaaa aagtttgcaa tttcccctct
     8581 ccctgatttt tcttctgaac gaagaaaaat ctatccctct cccacaaggg gagagggtta
     8641 ttgagtatca aatttatttt aaagaaggaa aacaaacaat ggcaacattt caaggaaact
     8701 acatcgcaac tggtttaaaa ttcggtattg tggcaactaa ctggcacaag atttttatcg
     8761 atcaactgtt aaacggtgca accgataaat tattgcgtca cggcgtagaa gaaaaaaata
     8821 tcgacaccgt atgggtgccg ggtgcattag aaatctcaat tgcagcgaaa aaaatggcac
     8881 aaagcggtcg ttatgatgcg attatctgct taggtgcggt cgtgcgtgga gcaaccagcc
     8941 actatgatgt ggtagtgaac gagtcagcaa aaggcatcag ctcttctgct ttagaaaccg
     9001 gcgtgccgat tattaacggt attttaacgg tggaaaattt agagcaagcg attgagcgtt
     9061 caggcactaa agccggtaat aaaggcgaag agtgtgcaat ggtagcgatt gaaatggcga
     9121 acctattaaa agccttagcg taatcagcga taaaatcccc ttagacccaa gagcctaagg
     9181 ggattttttt attttctgcg tagtaacagg ctggcaatga cacaaaatag gctgaaactg
     9241 taaattaacc agtgacctaa ttgtttaaac ggcgtatcgc ctttggttgg cacaatggtg
     9301 gcagttagtg catcagcagt aaattgcggt agttgttcaa ttacttttcc gttggcatca
     9361 acaatggcgg taataccggt attcgcagct cgaagcaatg gtttgccaag ttctaacgct
     9421 cgcattcttg ccatttggaa atgttgccaa ggcccgatgg aagaaccaaa ccacgcatca
     9481 ttggtgatag ttaataaata atctgaattt tggctttttt ggttttgttg taactgatca
     9541 ccaaaaataa tttcatagca aatcgccata ttaaatcggt tgtttttcgc caagaatgat
     9601 ggttgaacga aactgccttg tgaaagatta attggtagaa taaacacttc ccgcatccaa
     9661 tccagcaccg agccaaacgg cacatattcg ccaaacggca caaggtgatg tttgttgtag
     9721 cgagggttgc tgtgcagggc atattccctc tctttttcgc ctaatagcac gctactgtta
     9781 aacagttcct cttgctcatt ttgatagagt gtgccgataa taatttcact tccgtgttgg
     9841 cgagctaagt gatcgagcga gccaagcagc gggctgattt gattttcaag tgccggaatg
     9901 gcggattcag gcaaaataat cagttcactg ttacctaata ggggcagaat gagctgttcg
     9961 taggttcgta cgctgtggtt gaaatgttgc ggatcccact tcattttttg ctcaatattc
    10021 ccttgtacta aactcactac aagcggttgt ttttctgtat caatttgcac aaattggaga
    10081 aagcgagtgg caaaacttaa gccaaaaatg accgctagta ccatcaatga tggcacagtt
    10141 ttgttctctt gctgaataat tttcactaga tagctacttg caactattac caagaaagtc
    10201 aaaccttcaa cgcccaaaat cggcgcaata ccagcaaagg ggctgtctat taagctatag
    10261 ccgaattgta gccacggaaa accggtaaat accacgcctc gtaaatattc ggtaaaggta
    10321 aaaatcgcag ccaatgcaaa cggattattc agtttgagcc tgtgtgaaac tacactaaac
    10381 agcagattat aaatagcgag atagcacgcc aacaggaata ctgctatgta gcttactatg
    10441 gtaggaacac caccaaattg ggtcatactc acagaaaccc agtttacacc ggtgcagaag
    10501 tagccgatag accaagcaaa ggttgcccat aatgcggttt ttctgtttgc cagagtggct
    10561 gaccaaacca agccggctgc ggaagcaaag gcaagaatcc aaatatcaaa aggcgaatag
    10621 gcgaaagtgc cgattgcacc cagtatgatt gcaaggagac aagcggttaa aaaaggcgga
    10681 ttttttgcaa aattcataat aactcactaa ataaatgagg cacgccgaaa cgtgccttga
    10741 ttgaaactca ctatattatt cttcttcgtt aggagaggtt ttttccattt tctctaactg
    10801 ttcatcggaa agtgtgactc gtagctgaat caattggcgg ctattagctg aggtgacttt
    10861 aaaatcaatt ccgtttaact caatttgctc atctcgtttc gggaaataac cgaaagcctg
    10921 catcactaaa ccaccaatgg tatccacctc ttcgtcatta aagtcggtag caaaaaattc
    10981 attgaatttt ttgatgtcgg tgagcggtaa taccgcataa gtatgtcttg aaagctggcg
    11041 aattggtttg acctcttctt catcaaactc atcttcaata tcacccacaa tttgttcaag
    11101 aatgtcttca atggtgacta agcctgaaac cgcaccaaat tcatcaacca ctactgccat
    11161 atggaagcgt tctgaacgga actcttttag cattcggtct aggcgtttgc tttccggtac
    11221 aataaccgca gggcgtaata cctgttgcag attaaagggt tcagagtcag aacgtaaata
    11281 tttcaataaa tctttagagt gaagaatacc ttcgatagta tctttctcac cccgaatcac
    11341 cggaaaacga gagtgtgcgg attcaataat taaatcaata cagacatcta aaggttcatt
    11401 ggcatcaata aacacaattt gcgcacgggg aatcataata tcccgcactc gtaattccga
    11461 gatttccatt actccttcga tcatctcttt ggtgtcgctg tcgatcaatt cattttcaac
    11521 agagtctcga atcacttcga ctagatcttc acggttttta ggctcgtttt gaaaaagccc
    11581 gctaaataga gagtgtaaaa tcgatttttt ttctgatttt tcggtggtgt ttacactttg
    11641 ctgttcatca ctcatagttt gttccactta aataaagtac gcttataata agcaaattca
    11701 gtcacaaagc aaataaaaca ttgctatttt cactcaaatt agcataggat aagcaaaaat
    11761 ctttttattt cttcatattt taggagtttg aaatgaaatt aaccaaaata gcattaagtt
    11821 taacagcctt agttgtcagt tcgatgagtg tggccgggac gttaagtaca tcatcaatgg
    11881 tggacatttt agcctttgac gggcaacgtg ttaaatccgg cacgacttcg gttcaaatga
    11941 gtgagaataa aacgcatcaa gtggtagtgg aagtcggtgg gacagttgaa ggggattttt
    12001 tcacctctga tcaaatcatt ttgactttcc aaggcagtgc ggaaaatgca aaattagaaa
    12061 cgccaagatt aaattcaaag ttagatcttc gtaaattcca agagaatcag actgtggcaa
    12121 ttaaaaccga ttcaggtaag gttatttcac ataaacaaga tttcttaaaa ggggaaggtt
    12181 tccttgcgaa aacaagagtt gaagataatt taacgaaata taacttagcg aaaaaaacag
    12241 cttcagttga acagtttgcg acggcacctc ttgaagcaaa aggacaaatt gtggtggata
    12301 caaataaagt ttcacaagaa gaattacagc ttttatttag aaaggcagat aaagacaccc
    12361 aaaaacgttt cttagaatgg gcgaagaaaa atataaaata aacctatatc aattaataag
    12421 cggtcatttc tcgccaattt tttgcaaggt gttaaaaaaa tgaccgctca tacaatgaga
    12481 tttaccagca ataatagcag taacgccagc ctctatacat atagtctcta tcgtaattcc
    12541 aacgaatttc ttcaagttct acttgtgcct tgtaattttg ccaagcaagc ttataacagg
    12601 cttggaacat tggggcattg actttttcgg catagcgttt cttaaatgct ttctcttctt
    12661 gttcggtttg gctaagcggc ggattaaatt gttggtacat taactccgcc gtttctacat
    12721 cacattgttt ggcgagctga acttgcaaat cttgttctgc ccgaatttgc ttcgctttac
    12781 gttcagcttg ttgctctgga gttaaagtac agctacttaa cataagtgcg agtgaaaaaa
    12841 tgcagcctaa tttgagtgtt ttcattgctg aatcctctag ttgaaattgg tgatttattc
    12901 taccgcttct aactcacttt cttcaagttc atcagccata gcactaaggg catcacggct
    12961 aagttgtgcg agggctttat aaaaaccatt gtcgaaagct tctatttttc ctaagaattt
    13021 gcctgcacaa ggtagtaaaa attgttcgaa taatattttt tgagctaagg cagaatcgag
    13081 attatcttcg atccaagatg ccgtgagaaa tagtaaccca atgtgatcgg cattttctaa
    13141 ttcaggcatt gcccgctctt gtctaaaagc tacaaaatta ttaactgata atccataggc
    13201 tgaaattgtt ggagaaatag ccccattttc agcaaaaagc gcttgatagc ttgctgctaa
    13261 ttgcgtaggg ttagcattct tttgtaagag agtcagcgca ttttcacttt ggctatccgt
    13321 atttaatgcc cagacttggc gtaatcctcc ttgggcaagc cagttaaatg tgccagctaa
    13381 aatagggtcg gtagggtgac gataaaataa gttgccaaac aaacggcaaa gcagggaaaa
    13441 atcgttaatg gtagtttcgt tcataatttg aaatagatag aaaaaagaat tgttagtata
    13501 gcagaacaca agagaggact gaatgaaata tataggcgca cacgttagtg cttcaggtgg
    13561 ggtggaaaat gcagttttgc gttccgtaga aattggtgct aatgcatttg cgttatttac
    13621 taagaatcaa cgtcaatggc aggcaccggc attaaaagcc gaaactatcg aaaaatttaa
    13681 gcgtttttgt aaagtgcatc atttttcagc agagcaaatc ttaccacacg atagctattt
    13741 gattaattta ggaaatccgg aagcggaggc attggagaaa tctcgtgtag catttattga
    13801 tgagatgtct cgagcaaatc aattaggttt aaaattgttg aattttcacc cgggggcgca
    13861 tttaaagcaa atttccgagc aagattgttt ggctagaatt gcagaatcga tcaatattgc
    13921 agttgaaaaa gtaccgaatg ttattgccgt gattgaaaat acggcaggac agggttctaa
    13981 tttaggttgg catttcgatc atttggcaac tattattgag caagttgagg ataaaagccg
    14041 agtgggggtg tgtttagata cttgtcactt attttctgcc ggctatgata ttagttctta
    14101 tgaaaagagc gaggcagtat ttaatgattt taaccaaacg gttggttttc attatttaag
    14161 gggaatgcac ttaaatggtt caaaaacacc attaggcagc agagtggatc gtcatcatac
    14221 cttaagagaa ggcacaatag gaaccgaagt gtttgcattt attatgcaaa atatgcattt
    14281 taaccatatt cctatgattt tagagacaat ttcgcctgaa atttgggcag atgaaataaa
    14341 atttctacgc acattggaac taaaataggt tatactggtc tgagtagtgg gtctagcaga
    14401 gtctagacct tattttgtta tcttttttaa tcttttagaa gatttagtta aggaatttac
    14461 cgctgtaatt gttctggcga gacgagacat aaattatgaa aaaaaatctt tttgcaacaa
    14521 ccttcaagcc cgtatttatt gcttctattt tgtctgtttt tggagggtat tcttccattg
    14581 ctgtagcaag tttaagttct aaaccggcca caccaaatca aatcattgca agtaaagcag
    14641 aaagattagc taagtcttct tcagtattta ccaatcgagc aaatcttcca acttcttccg
    14701 ctcatacagc gaattatagt ggtcgtatta atagtgttta tcataattgg gtagggacac
    14761 gctatcgttt aggcggcaca acaaaagcag gaattgattg ttccgccttt gtgcgtgaag
    14821 taatgggcaa agcatttagt aaagatatgc ctcgttcaac tgcagaacaa cgccatgtcg
    14881 ggcattccat ttcaaaatct gatttacgcc caggtgattt agtctttttc cgtaagaata
    14941 accatgtagg ggtttatatt ggtaatggta aatttgtaca cgctagttca agtagagggg
    15001 taaccaccag ctcattgtca gaaagctact ggtcaagaaa ctacacacaa tctcgccgtg
    15061 taatttaata aattacaatt ccaagatgaa aagaacactc acttttgcga gtgttctttt
    15121 ttaattaaaa aagtttatgc tacgcgttgc ttagcttgtg taaatgctcc acgagtacgc
    15181 atttgatgaa ccacattagt aattacacct gctattttta cttcagccca atcagtaatt
    15241 ctaagatttg attcgctcac atctaatgaa aataaaattt tttcagcaat accttcccca
    15301 ttctgaattt cattaaagaa ttgatagaat ttatattccc catctttttc aagcaccaat
    15361 agatcattaa ctaaatattg gttggtctgt tctacaatca ataaatcgtc tagttcaatt
    15421 ccccacgcga ttaaatttgg gtttttcacg tggataaaaa aagtgtctct tgggcgtttg
    15481 atacaaagtg agttaagatc gagctttaca gagcggctgc tattgaaatc ccgataaagc
    15541 ggaatcggct gataagctgc cgtttgatct gaaatttgtc taattgaagc catagtaaaa
    15601 tcctctatct gtttttttat acagggtatt ttactggcta aaataccagt gtcaaccatt
    15661 attttggaat tttttgtaaa tttaaccagt gttttgaccg cttgttatcc tttaaaaagc
    15721 aataaaaaag cacccatagg gtgcttctta tgcttaaaag ctgttattat aggctttcag
    15781 tgaatgtacg agtaataaca tcgcgttgtt gctcaggggt taatgagttg aaacgaactg
    15841 cataacctga aacacggatc gttaattgag gatatttctc agggttttga atcgcatctt
    15901 ctaatgtttc acggcgtaaa acgtttacgt ttaagtgctg accaccttca actttaatcg
    15961 ttggtgcaac attaacgggt acttcacggt atgcgatatc acctaattcg ctgatagcaa
    16021 caacttgatc ttcttcaaat cctgcttttg cagctaaaca acgagcttca tttttagctt
    16081 cgtctaataa ccagaaagag tttaataagt tgctgtttga tgattctgtg atttgtacac
    16141 ctttaatcat tttgatatct cctctaagat tgaaattata aaaaaatttt aaaaattcaa
    16201 atttatttga agttattgta ccccttttta gataaaaagc gataaaaatt taaccatttt
    16261 tagacatgat tgagttggtt gggtaaaaaa tatacacttg gtggttttat gaaaacttgg
    16321 acagaagctt taggcgaaga aaaacaaaaa gcgtattttc aacaaatttt acaatatgta
    16381 cataacgaac gattagccgg caaagtcatc tatcctccgc aaaacgaggt gtttagtgct
    16441 tttgccttaa ccgaatttag tgacgtgaaa gtagtaattt tagggcaaga tccttatcac
    16501 ggcccaaatc aagcccacgg attagccttt tcagttaaac cgccggttgc cccgccacct
    16561 tctctggtca atatgtataa agaattatct gaagatattg aaggatttca aattccaccg
    16621 catggctatt taattgactg ggcaaaacaa ggggtattta tgttaaacac cagcttaacg
    16681 gttgagcaag gtagagccgg ttcgcattca caaattggtt gggaaatttt taccgataaa
    16741 gtgatcgcac aattaaatca acaccgtgat aatttagtct ttctactttg gggaagccac
    16801 gctcagagaa aggggcaatt tatcgatcgt aatcgccatt gtgtgttagc ttctccgcac
    16861 ccatcaccac tttccgcata tcgtggattt tttggttgta aacatttttc taaagccaat
    16921 aactacttga tttctcaagg aatatcccca attaattggc aattatcagt agacgttagt
    16981 tattcttcaa cacattagtt taaggagcaa acgatgaatt ttgatttacc gattgttagt
    17041 attgcatttg ttgtgcttgt tttagttgca ttaagttcga ctattaaaat tgtgccacag
    17101 ggctatcatt ggacagtaga acgttttggt cgttatacta aaacgctaag cccggggttg
    17161 aacattgtcg taccttttat tgatcgtatt gggcgaaaaa tgaatatgat ggagcaagtt
    17221 ttagatattc catcgcaaga ggttatttca cgtgataatg caagtgttgc gattgatgct
    17281 gtatgttttg ttcaaacagt tgatgctcgc cgagctgcct atgaagtgaa tcatttagag
    17341 caagcaattg ttaatttaac catgacaaat atgcgtaccg tgctcggctc tatggatcta
    17401 gatgatatgc tctctcagcg tgatttaatt aacggtagat tactttccat tgtcgatgag
    17461 gcgactaata tttggggagt aaaagtcacc cgtattgaga ttcgggatgt tcgtccaccg
    17521 aaagagctgg ttgcggcaat gaacgctcaa atgaaagctg agcgtaataa acgtgctgat
    17581 attcttgaag cagaagggat tcgacaagct gaaattctgc gggctgaagg ggaaaaacaa
    17641 tctcgtattt taaaagcaga aggtgaaaga caagaagcat tcttacaggc agaagctcgt
    17701 gaacgagccg cagaagcgga agctaaagcc actcaaatgg tttctgaagc aattgctaaa
    17761 ggcgatacta cagcgattaa ctattttatt gcacaaaaat acaccgaagc tttgaaagaa
    17821 ataggttctt cagataacag taaagttgta cttatgccgt tagaagctgg aaatttgatt
    17881 ggttctgttg ctggtattgc tgaaatttta aaaagcaata aaacaagctc atagcctaaa
    17941 accggattaa ttgagtatat ggagggagta tggactggtt ttttaattgg attggctgga
    18001 gcatattagg ctttgtacta cttgcattag aattagtatt accgggtgtc tttattatgt
    18061 ggtggggatt tgcctcgctt attcttgctt tcatcgttgc actttttaca ttagggcaag
    18121 cgttacaaat tgcgttattt gcgataattg cgactatttt tagtcttatt tggtggaaat
    18181 atcagcatgg aaaagacaag aaagaggatc agagcagttc ccttaactct cgagagcatg
    18241 ctatgctagg agctcaaggc tttgtggttg aaatcttacc taatggcatc gctcgaggaa
    18301 aatttggtga tacgacttgg cgtgttatag gtaataattt acaagtagca gataagattg
    18361 aagtccagaa agtggaggga attaccttat ttgtccgtaa gcaataagtt ttttcttatt
    18421 tagtcataga acactaaaca aaaaccttga acagttgttc aaggtttttt ctctcaagcg
    18481 gttgaaaata gagtgaaatt tgcaaatttt caccctaaat taaccgcttg tgtcccacca
    18541 taataagtta gaaactaaag ccaacattta ctttccaacc aaataggttt gcagatttca
    18601 ctcgactgta atctaaattc atcgcagtaa accaattttt ataatgcagt ttcaccccga
    18661 cttcgccgtt taattggcga ttacgtttcg tcgttgcttc actatgcagt gtttttcctg
    18721 caatgttact ggtaattttg gttatttttc catttaatct atgcaagtaa tttagcccga
    18781 tataagggga aatagagcct tgctcaagtt ggaaagttcg ctgtaatttc acgcttggaa
    18841 caatattcac atcacgataa cgcattgcag ccgtttgagt cgcaagttcg ccactttcat
    18901 ttaaaccatt cattttggtt tgaatccatt gtacgccaag gcttggttca attatccatt
    18961 ggtttggcat aaattgataa ccgatccgca tttcaccacc gagcgttgaa cctctggttt
    19021 ctgcagtacc tagttgatgg ctagcaaatc gacggctttg ttgatatttc tggcgagcaa
    19081 attgcaatgt agcttcacta aaccaagcgt tatctaaatg ataacgtaag ccaatattta
    19141 atgctgtttg ttttaaggta gcttgtgcaa atggtttttc taagcggttt ttttgctgtg
    19201 aaagtgttgc actaagtacc gttttatctg ccagtttgtg gtttactccc aattgtgatt
    19261 ggtggtgttt aacatcggta tgcttcgatg aggctgattg tttttgattc aaatgttcag
    19321 cccaaacagt cgtttgcagt ggtgtatggt atgccgtaaa caaatgttgc tgtgcttggg
    19381 ttaagccttt ggcattttgg ctacgtagca attcaaggct atctgcataa atattgttag
    19441 atacagcata gtgcagatta tctaatttac gttgttcgag tgcagattgc agccaaccgt
    19501 tataccaact acggcgatag gcaataggtg tagttcttaa cgcatttaat aatgctaaac
    19561 cggcttgccc gtttgtatcg gctttctcat cagttagtgg cttattcact tttgcgataa
    19621 tttcgctctc tttgaaatag agatcttgga ttaaccctcg tttggctaaa ttctcgtttt
    19681 cgactaattg tgtaaatccg ccttgataag ttgctttatc tttcaactgt actacggtcg
    19741 ctgaaacatc acctgcttcg gttttttctg ttaatttatc atcaattttt aacgagccgg
    19801 ctaaatgtat attggcttga gcattggcaa ttagcccaga tttgcccaac acttctaatt
    19861 ggctttgtcc agacgaagaa taattttgct caacttcggt tccattgaca gctaaatttg
    19921 catggttact gattgttttc accttagttt taccgtttaa ggccaatcta ccttcttcaa
    19981 ctgctaccgt atctaaatga ttttcgccgt ctaaatgcag tgatttgtcc cctttttttg
    20041 taattttaaa ttggctggcg agtggattcg accaatggtc atcacgagtt acggtaatcg
    20101 tttcatcatt taggaattga gttgggccat taacagcttt tttcaggtta atgagacccc
    20161 agccataaac attatcgacc cctttttcac ctaaatcagt ggcggtagtc aataaagtat
    20221 cgcgaatttg agtcgcagtc agataatcaa agcgttcttt taatactgct aatgaagcag
    20281 ttatagcagg tgctgaaaat gacgtacctt gctcttttgt taagccatat tgtggttttt
    20341 tatgttcatc agcaacgcca attaaaacat gtaaatcgcc cggtgctgct acacaccagt
    20401 ttttacttgc tccgcagtgg ttagagtatg aagcaatttt ttgttcttta tctaccgcaa
    20461 cggcagaaat taaatttttc tcaagctcgg gaaagtaacg tggtagaaga gccattacgc
    20521 ctggctgttt cttgctctca ttaccagcag caaagactaa taaggtgttg ctatttttgg
    20581 ctctcataat agcttgaagt agcggattgt gcttattttg cttgatagat tgataaaatt
    20641 tatctacaac attgatgtca tcgctaatag aaaaaggatt ccaactatta ttgattgcaa
    20701 aaacattatt atctagtaat ttattcagcc cggatgccca ttcagcacgt tcaattgcca
    20761 tttctttttc tttgcttgct aataagtcat tctttgccat ttgatccaga cttggcgttt
    20821 tttcatcctc ttcgtctttt gcattttcta attctttctt agcatcttga ataacttttt
    20881 ctaaagttcg tttatatgac ttcgttgcaa cataaagctc agctcccttt gcaataccac
    20941 cactatagcc atcaccaaga gttttatctg cttgagcggc aataatacca gctacacctg
    21001 caccgtgtgt ttcaagagaa ggaataagat caattccatc ttctttttct acggggttta
    21061 cctcgaattg agggtaactt gcatcgtaga ttcttacttc gccttcgggt gttgttaatt
    21121 gatgaataag aggatgaaga tgtgaactat ggcgagggtg tttaaccatg aagccctcat
    21181 ccattacgcc tagtttaaca cccttgccac tatattttga gctgaggctc tcagataatt
    21241 gaatgatagg ttgtggattc tctattgatt ctgtagcgta tgcaatagat aaaaaactag
    21301 aaatgctaat tgcaataagt gttttgttaa atgaatgctt tattttatac a
//
LOCUS       DANLXS010000018        21272 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.018, whole genome shotgun sequence.
ACCESSION   DANLXS010000018 DANLXS010000000
VERSION     DANLXS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21272
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.018"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..167)
                     /locus_tag="PXD71_000557"
     CDS             complement(<1..167)
                     /locus_tag="PXD71_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6290714.1"
                     /translation="MKAQQLKNAILQLAIQGKLVPQDPNDEPASELLCKIQAEKDRLI
                     AEGKIKKNKKTA"
     gene            complement(373..2034)
                     /locus_tag="PXD71_000558"
     CDS             complement(373..2034)
                     /locus_tag="PXD71_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL6290715.1"
                     /translation="MLTWEHIEKMIAHQQFIREKAEIEYKEAKNSVPKDLWHTYSAFA
                     NSNGGFILLGIKENEQGHFPELLISGVNDPDKVIDELFSQSRSEKVSKSLLKDNDVKI
                     FQVENKSVIAIYVKAADILDRPIHLNGDYRLSYVRLNSGDHKLTSSELRSFISSYSQK
                     DADGRIVPYASIDDISLSTLDKYRNLLKAHNSTSKLLTLSDEKLLQEIGAYKKDLAQN
                     SEGITNAGLLMFGKHYAITSIFPHFFFEYYEEAGDVKRYTLRITDFDLEEGNLFEFYL
                     KIVPLIQELGKDKHFKLDNLTRTEENEITDGLREALINAITHADYFNNVRHLKVIKTK
                     DNKLIFENAGVMLVDIPTAIAGQRSECRNSIIHNLFHRLGLCERQGQGVRDIYSYWQS
                     RNFNSPLLESEIDYTKLTLTFQSGMIADFEEKFTACFGENFSELQPIQKDCLLFIAFN
                     QGKAMFAELAQKLPQYPRRNITLALARLKEKGLLTSSGSPRKQLYTLVNLEWIITEKS
                     QDDYFNPPFDQLDKASESKGAPPKKSKVKQVKQLPQNKANKLPPK"
     gene            complement(2047..3516)
                     /locus_tag="PXD71_000559"
     CDS             complement(2047..3516)
                     /locus_tag="PXD71_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL6290716.1"
                     /translation="MSLNNIVKRLQDVMRNDAGINGDAQRIEQIVWILFLKIYDAKEQ
                     EWELENDEYHSILPNFLRWQNWAEDKKDGKAMTGDELLSFVNNELFPTLKNLPISADT
                     PMNQRIIRAAFEDNNNYMKNGVLLRQVINIIDEIDFAHYQERHAFGDIYENILKSLQS
                     AGNAGEFYTPRAVTDFMVQAIKPKLGERIADFACGTGGFLTSALKVLESQIQTLSDRT
                     LFNNSVYGIEKKALPHLLCITNLLLHDIDNPNVHHDNALEKSVKDYTENDKFDVILMN
                     PPYGGSEIEQIKKNFPTALQSSETADLFMSVIMYRLKQNGRAAVVLPDGFLFGTDNAK
                     VAIKKKLLEEFNLHTIIRLPHSVFAPYTSITTNILFFDKTEPTRETWVYRLDMPQGYK
                     NFSKTKPMKLEHFNEVVEWWGNRQVIEVEGFDKAKSFTLKEMSDRQYNLDLCGFPHEE
                     EEILPPDELIADYQRKRTAYNADIDKILAEITDILGIKL"
     gene            complement(3564..5915)
                     /locus_tag="PXD71_000560"
     CDS             complement(3564..5915)
                     /locus_tag="PXD71_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL6290717.1"
                     /translation="MYTLQELKQMNEQEIRSRLISPAIRNAGWSDRQIGEEYTFKTNK
                     RFTDGQVVVDPKTTQTKRIEAKRVDYLLYTSANQKIAVVEAKDNHHSSRHGLQQAMTY
                     ARLLDVPFAYSSNGDEFVEHDFITGVQRTLPMSAFPTPDELHQRWAKQYTTEELKIIQ
                     QPYYTSSTDTFKEPRYYQEIAINRTVEAVAKGQQRILLVMATGTGKTYTAFQIIHRLK
                     TSGAKKKILFLADRNVLVDQPMMQDFKPFKDKMVKVSNKKLDSAYEVYLALYQQLDGE
                     TDNELFKQFQPSFFDLIVIDECHRGSAKEDSDWRAILKYFKDATHIGMTATPKETDDA
                     SNITYFGEPIYTYSLKQGIEDGFLAPYRVIRVDLDCDLKGWRPTKGLKDHQGNEIEDR
                     LYTVKDFDYNIVLNDRTRLVAERICEFLRKTDRLQKTIVFCANINHAERMVAELSRLN
                     ADMLKHNSKYVCQITGDQSGKGVDLEEFCDVANEGKDYPVIAVTSKLMTTGVDSKTCK
                     LVVLDANIQSMTEFKQIIGRGTRLRPDFGKEFFTILDFRGVTRLFADPAFDGEPVVIL
                     DVPENKPLVEQPLPKPSDKDIIKDPELKPYKIPVVVDFGTLKLRILDEKVQYLNEQGE
                     LVSEDIDLFSKREMQKEFGSYEQFQQQWTTSGEIFSKFYTDPKWLAALRQTRQFSHDV
                     EDFDVLSHISFGKKPLTKTERAEKVKQSGYVEQYSPENQQVLGLLLNEYVKSGNQDLT
                     KTEILKTPQFSRFGGLVSIIKNFGGKELYQKAVKGLEKMIYSE"
     gene            complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PXD71_000561"
     CDS             complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PXD71_000561"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251643.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL6290718.1"
                     /translation="MQLKKYPVPTYFQRVKIALHYLLPQLAITRAAGWLAEQKWGVVT
                     HLIIKLFAKQYKVNLSEAKKTEPSDYATFNEFFIRELKENARPINQDPNTLCLPAEGK
                     VSESGEISDNRLLQAKGHHFTLETLLANDEEMAAKFKDGTFITTYLSPTDYHRVHMPC
                     DGTLRKMIYVPGELFSVNPFLAEHVPNLFARNERVICEFDTAFGPMLQILVGATVTAS
                     MSTVWAGVINPPRADEVVVWNYETEGEKAIKLQKGEEMGAFRLGSTVINLFPKGKVHL
                     NPALIAGMQTRMGEELGRIIN"
     gene            complement(7045..7383)
                     /locus_tag="PXD71_000562"
     CDS             complement(7045..7383)
                     /locus_tag="PXD71_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDL6290719.1"
                     /translation="MKKIKQLGLTFLFGLNAMAFAQTPPTTMPVEQNAVTQPVQQTQI
                     TNANAVNINTASAAELQDKLVGIGQKKAQAIVDYRTKNGNFKNIEQLAEVSGIGQATV
                     EKNRANIILE"
     gene            7676..8995
                     /locus_tag="PXD71_000563"
     CDS             7676..8995
                     /locus_tag="PXD71_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="opacity-associated protein A"
                     /protein_id="HDL6290720.1"
                     /translation="MTQNNFRKEPVFGESSVTSQDAEATKTDTAQAPYVSLRSTKAPG
                     HTFTPIMKRPDAKVEDHTADVFSAKQAKEFQFTPSSEATEAVEPDNTIHSVEESVSTA
                     SFNAVEAQENVEKEVVKPAPESFTTERIIPNAATAAAAATAAAAATKTKEIKAKMPPK
                     TRRLLLVALLALALLVLFFLLKPSTPETVEELQSQQGGSLPIEFRPVDEAEAKRAEEQ
                     ARAEQEAAEAAKAQEQAQAAQQESVTVAPAQTEQTMAKPSQDSQSQTQSQAVVEQPVA
                     VKPVENKPVVAQPAIKPKTENSVVFQPETTVKQQPKAPAKTEVKTERATLAAKETAKT
                     AAKPVEKAPTKTEAVKAAPAAESANAVATKTMTVPKGVSLMQVFRDNNLNISDVNAMS
                     KVNNVVSNLKVGERVTVRLDKNNRVVEMSIGSGGKFTRQANGSYTFK"
     gene            9113..9475
                     /locus_tag="PXD71_000564"
     CDS             9113..9475
                     /locus_tag="PXD71_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HDL6290721.1"
                     /translation="MLVACSTVVTPVEQIHQVQKEKESVKKVTKLIAQAPKVSLYRCE
                     NDKKVEVERQGRTKKALKKEIVTVSYQGTTHQLSPSVTRDGKKYTNIRWTWHETRSGK
                     AFLYNNTKKTLAANCIKQ"
     gene            9586..10623
                     /gene="waaF"
                     /locus_tag="PXD71_000565"
     CDS             9586..10623
                     /gene="waaF"
                     /locus_tag="PXD71_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HDL6290722.1"
                     /translation="MNILVIGPSWVGDMMMSHALYQQLKIQYPNCQIDVMAPDWCCPL
                     LARMPEVRNAISMPIGHGSFRLCERYRLGKSLRNQYDMAIVLPNSLKSAFIPLFAKIA
                     RRRGWKGESRYFFLNDLRSNKNDYPMMVQRYVALAFEKATVPTAQQLPIAYPYLTTDS
                     EQIEQTKAKFEKQLAYAENRPAIGFCPGAEFGPAKRWPHYHYAILAKMLIEQGYSVRL
                     FGSKKDEAAGEQIRLALPENLQRYCLNLAGQTDLNQAVDLIADCNAVVSNDSGLMHIA
                     AALNKPLVALYGPTSPQYTPPLSQNAEIMRLIEGGLIKIRKGEGAEGYHQSLIDIQPE
                     MVMDKLAELLV"
     gene            10690..11649
                     /gene="rfaC"
                     /locus_tag="PXD71_000566"
     CDS             10690..11649
                     /gene="rfaC"
                     /locus_tag="PXD71_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDL6290723.1"
                     /translation="MKILIVKTSSMGDVLHTLPAITDMQNAIPDLKVDWVVEENFTEI
                     PAWHSAVNKVIPVAIRRWRKNLCQRQTWVECQQFLKQLKSEQYDAVIDAQGLIKSAVL
                     VTRQAKGKKFGYDKNSAREGLSSLFYDETFNIPYQQHAVERIRKLCAKSLGYALPTER
                     GDYCIAQHFAKNPQKTTAYIMLIHATTRADKHWQEAYWAELIQALDKHNLAVRLPWGN
                     QAEKERAERLASVANNAKVLPKMSLTDIATQLAGAAAVVSVDTGLSHLAAALDKPNVI
                     LYGATDPKLIGAYGQNQFYLKADKMENITSSQVLEKLLPLINK"
     gene            11770..12144
                     /gene="rpsL"
                     /locus_tag="PXD71_000567"
     CDS             11770..12144
                     /gene="rpsL"
                     /locus_tag="PXD71_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL6290724.1"
                     /translation="MATINQLVRKPRVKKVVKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRIRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVKDRKQGRSKYGVKRPKA"
     gene            12275..12745
                     /gene="rpsG"
                     /locus_tag="PXD71_000568"
     CDS             12275..12745
                     /gene="rpsG"
                     /locus_tag="PXD71_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL6290725.1"
                     /translation="MPRRRSIEPRKILPDPKFGSELLAKFINVLMVDGKKSTAEKIVY
                     GALETLTQRSGKEALEAFEIALENVRPTVEVKSRRVGGSTYQVPVEVRPTRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAADNKGSAVKKREDVHRMAEANKAFAHFRW"
     gene            12847..14949
                     /gene="fusA"
                     /locus_tag="PXD71_000569"
     CDS             12847..14949
                     /gene="fusA"
                     /locus_tag="PXD71_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL6290726.1"
                     /translation="MARTTPISLYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMSQQYPQHRINVIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRTGANFLRVVEQIKT
                     RLGGNSVALQLPIGAEDSFKGVVDLIKMKAINWNEADQGMTFTYEDIPADMLEACEEH
                     RAMLVEAAAEASEELMEKFFSGEELTEDEIKAALRQRVLAGEVIPVCCGSAFKNKGVQ
                     AMLDAVIDYLPAPTDIPAIKGINEDETEGERHASDDEPFASLAFKIATDPFVGNLTFF
                     RVYSGVINSGDTVYNSVKQKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTG
                     DTLCAIDAPIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEE
                     SGETIISGMGELHLDIIVDRMKREFKVEANIGKPQVSYRETIRTRVNDVEGKHAKQSG
                     GRGQYGHVVIDLYPLDAEGPGYEFVNEIKGGVIPGEYIPAVDKGIQEQLKSGPLAGYP
                     VVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFAKASPVLLEPIMKVEVETPPDYV
                     GDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLK
                     YAEAPKNVADAIIEARRK"
     gene            15009..16193
                     /gene="tuf"
                     /locus_tag="PXD71_000570"
     CDS             15009..16193
                     /gene="tuf"
                     /locus_tag="PXD71_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL6290727.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKHFGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTATRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALQALNGVAEWEEKILELANHLDTYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIRTGDEVEIVGIKDTAKTTVTGVEMFRKLLDEGRAGENVG
                     ALLRGTKREEIERGQVLAKPGSITPHTDFESEVYVLSKEEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMTVSLIHPIAMDEGLRFAIREGGRTVGAGVVAKI
                     IK"
     gene            16490..17788
                     /gene="tig"
                     /locus_tag="PXD71_000571"
     CDS             16490..17788
                     /gene="tig"
                     /locus_tag="PXD71_000571"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL6290728.1"
                     /translation="MSFSIETLEGLKRRVTINVPADKIEAAYKEQLKGYAKNARVDGF
                     RKGKVPHSIIEQRFGIAARQDVLSDEMQRAFFEAVIAEKINLAGRPTFTPNNYQPGQD
                     FSFTAEFEVFPEVELKGLENIEVEKPVVEIADADLDKMVDVLRKQQATWSESQEAAKA
                     ESRVTIDFVGSVDGEEFEGGKASDFVLFMGQGRMIPGFEDGIVGRKAGEQFDIDVTFP
                     EEYHAENLKGKAAKFAITLKKVEEMVLPELTEEFVKKFGNAKSVEELRAEIKKNMQRE
                     LKNAVTSRVKTQVINGLIAQNDVEVPAAAVEEEINVLRQQAVQRFGGKPEMAAQLPAE
                     LFEAEAKRRVQVGLLLSSIIGSNELKVDEKRVEEMIADIASAYEQPAEVVAHYAKNRQ
                     LTENIRNVVLEEQAVDAVLAKAKVTEKTMSFDEVMSQQAQ"
     gene            17904..18356
                     /locus_tag="PXD71_000572"
     CDS             17904..18356
                     /locus_tag="PXD71_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL6290729.1"
                     /translation="MEQLTFAQQLEQFFANHTLMVFAWVALLIAVIFSFYKSATQKYK
                     IVDNAQATQLINKEEAVVVDLRSDEEFRAGHIIDSIHVLPSDIKAAKINQIDKYKARP
                     VIVVDTNDFTSGGVAADLAKQGFSRVYVLKEGIGGWRAASLPTIKKHK"
     gene            18377..18877
                     /gene="secB"
                     /locus_tag="PXD71_000573"
     CDS             18377..18877
                     /gene="secB"
                     /locus_tag="PXD71_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDL6290730.1"
                     /translation="MSEENQVASTEEATQTPFELQIQRIYVKDVSFEAPNLPTIFQQE
                     WKPQLGFELDTETKEIGEDLYEVTLHITVQTTLEDSNDVAFICEVKQAGVFTIKGLEG
                     IQLAHCLASQCPNVLYPYARELISSLVNRGTFPALNLSPVNFDALFMDYLQRQEESAE
                     EPALAN"
     gene            18984..19994
                     /gene="gpsA"
                     /locus_tag="PXD71_000574"
     CDS             18984..19994
                     /gene="gpsA"
                     /locus_tag="PXD71_000574"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL6290731.1"
                     /translation="MSEIYSAPITVLGAGSYGTALAIALSRNGHKTYLWGHNPDKMAV
                     LASERMNQDFLPDIVFPEALEIVADLATAVQKSKDLLIVVPSHVFSEVLAQIKPFLSQ
                     DHRIMWATKGLERDTGRLLQTVAKEILGEIYPLAVLSGPTFAKELAAGLPTAISLAST
                     DPVFADQMQKRSHCSKAFRVYLNNDIIGVQLGGAIKNVIAIGAGISDGMGFGANARTA
                     LITRGLAEISRLGVSLGADPKTFTGMAGLGDLVLTCTDNQSRNRRFGLALGQGKSAEV
                     AIAEIGQVVEGFYNTKEAYLLAQTQGVEMPIVEQIYQMLFCGKQASDVVKTLLGRERK
                     GE"
     gene            20010..20825
                     /gene="cysE"
                     /locus_tag="PXD71_000575"
     CDS             20010..20825
                     /gene="cysE"
                     /locus_tag="PXD71_000575"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL6290732.1"
                     /translation="MQQSEINQIWQAIRDEAKELANCEPMLASFFHATILKHHNLGDA
                     LSYILANKLENPIMPAIALKEIIEEAYRAEPQIIASAACDINAVRTRDPAVDKWSTPL
                     LYLKGFHALQSYRVTHYLWNQGRKALAVYLQNEISVAFDVDIHPAAKVGCGIMFDHAT
                     GIVVGETSVIENDVSILQGVTLGGTGKENGDRHPKIREGVMIGAGAKILGNIEIGRYS
                     KIGANSVVLQPVPDHATAAGVPARIIGKSSEQKPAFDMNQYFEDEQGLFGDGI"
     gene            20874..>21272
                     /locus_tag="PXD71_000576"
     CDS             20874..>21272
                     /locus_tag="PXD71_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6290733.1"
                     /translation="MQLTFNDFQNYYNGKGDLTMFSFYGKKTWENGIYVGIDGSIGRA
                     NNKLSHQSQTIKLARNVYSLGLTVGKTLEVASFNLQPSFGMRYHHLTSANAQLENTKF
                     ETDRVDLLAIQAGLALNKTFIFNELKVKPEL"
BASE COUNT         6425 a         4080 c         4573 g         6194 t
ORIGIN      
        1 gcagtttttt tgtttttttt gatctttcct tctgcaatca agcggtcttt ttctgcttgg
       61 attttgcaaa ggagttcgct tgccggttca tcatttggat cttgtggaac gagcttgcct
      121 tggattgcta gttgtaggat tgcgtttttt agctgttggg ctttcatttt ttattctcaa
      181 taatcggttg ttatttctta aagtatcttt gattacggtg ttttgggttt tctgggaatt
      241 gcatttcgat cacaccaagc gttagtgcag gttttaaata ctgcatacgg aaatgcttat
      301 catcttttaa cctgagtttt ttcataattt ctttgctctt catcggctca agcaacactg
      361 aaagtaagcg atttacttcg ggggtaattt gtttgctttg ttctggggta attgtttgac
      421 ttgcttcact ttgcttttct tcgggggggc gcccttactc tcactagctt tatcaagttg
      481 gtcgaaaggt ggattaaaat aatcatcttg tgatttctct gtgattatcc attctaaatt
      541 cactagggta tagagttgtt ttctcggact ccccgaactg gttagtaagc ctttttcttt
      601 cagtcgtgct agtgccaaag taatatttct gcgtggatat tggggcagtt tttgtgctaa
      661 ttctgcaaac attgctttac cttgattgaa ggcgataaag agtaagcaat ctttttgaat
      721 aggctgtaac tcagagaaat tttcgccaaa gcaagcggta aatttttcct caaaatctgc
      781 aatcatacct gattgaaaag ttaaggtgag tttagtgtaa tcaatttcac tttctaaaag
      841 cggtgaattg aaatttcggc tttgccaata tgaataaata tcccgaacgc cttgtccttg
      901 tcgctcacat agccctaaac ggtggaataa gttatgaata atagaattac ggcattccga
      961 tctttgccct gcaatagccg tgggaatatc aaccaacatt actcctgcat tttcaaaaat
     1021 cagtttgtta tctttggttt taatgacttt tagatggcga acattgttaa agtaatctgc
     1081 gtgagtaatt gcattgatta gagcttcccg tagcccgtca gtaatttcat tttcctcagt
     1141 acgggttaaa ttatctaatt tgaaatgttt atctttacca agctcttgaa taagtggcac
     1201 aattttcaag taaaattcaa agagattgcc ttcttcaaga tcaaaatcgg taatccgtaa
     1261 tgtatagcgt tttacatcac cagcttcttc gtaatattca aaaaagaagt ggggaaatat
     1321 gcttgtaatg gcatagtgct taccaaacat caataagcct gcattggtaa tgccctcgct
     1381 attctgtgct aaatcttttt tataagcccc aatttcttgc agcaatttct catcggataa
     1441 cgtaagtaat tttgatgtgc tgttatgggc tttcagcaga tttctgtatt tatccaatgt
     1501 gcttaaactg atatcatcaa tagaagcata aggcacaatt ctcccatcag catctttttg
     1561 gctatagctt gaaatgaaag aacgcagttc agaggaggtg agtttatgat cgcctgaatt
     1621 taaacgaaca taagaaagtc gataatcacc atttaaatgg ataggacggt ctaaaatatc
     1681 agctgctttc acataaattg caatcacgct tttattttca acttgaaaga ttttcacatc
     1741 attatccttt aataaggatt tactgacttt ttcactacga gattgggaaa ataattcatc
     1801 aatgacttta tcaggatcat taacaccaga aattagcagt tcaggaaaat gtccttgttc
     1861 attttccttt attcctaata aaataaagcc accattgctg tttgcaaaag ccgaataagt
     1921 atgccaaaga tcttttggca cactattttt tgcctcttta tattcaattt ctgccttttc
     1981 tcggataaat tgctgatgtg caatcatttt ttcgatatgt tcccaagtca gcattttatg
     2041 ctcctattac agtttaatgc ctaaaatatc ggtaatttct gctagaatct tatcaatatc
     2101 agcattgtaa gcggtgcgtt ttcgctgata atctgcaatt agttcatcag gcggaaggat
     2161 ttcctcttct tcgtgaggaa agccacataa atcgaggttg tattgacggt cggacatttc
     2221 ctttaatgta aaacttttcg ctttgtcaaa cccttcgact tcaatcactt ggcgatttcc
     2281 ccaccattca acaacttcgt tgaaatgttc caatttcatc ggtttagttt tggagaaatt
     2341 tttataaccc tgtggcatat caaggcgata aacccaagtt tcacgggttg gctcggtttt
     2401 atcaaaaaac agaatattag tcgtaatcga ggtataaggg gcaaagacac tatgcggtaa
     2461 gcggataatc gtatgcagat taaactcttc taatagcttt ttcttaatcg caactttggc
     2521 attatctgtg ccgaacaaaa atccgtcagg cagcaccacc gcagcccgtc cattctgttt
     2581 taggcggtac ataattaccg acataaacag atcagcggtt tcactgctct gtaaagccgt
     2641 agggaaattc tttttaattt gctcaatttc tgaacctccg taaggtggat tcatcaaaat
     2701 cacatcaaat ttatcatttt cggtgtaatc tttgaccgat ttttctagag cattatcgtg
     2761 atgtacattc ggattatcaa tatcgtgcaa caataaattg gtaatacaaa gcaggtgagg
     2821 aagggctttt ttctcaatgc catacacaga attattgaat aaagtgcggt cagaaagggt
     2881 ttgaatttga ctttccaata cctttaacgc cgaagttaaa aaaccacccg tcccacaggc
     2941 aaaatcggca atgcgttcgc caagtttcgg tttgattgct tgtaccataa aatcggttac
     3001 agcgcgaggg gtataaaatt cccctgcatt acctgcactt tgtaaacttt tgaggatatt
     3061 ttcataaata tcgccaaagg cgtgtcgttc ttggtaatgg gcaaaatcaa tttcatcaat
     3121 gatattgata acctgacgta ataacacccc atttttcata tagttgttat tatcttcaaa
     3181 agcagcacgg ataatccgtt ggttcatcgg cgtatcggct gaaataggca gattttttaa
     3241 agtcgggaaa agttcgttat tgacgaaaga gagtaattca tcaccagtca tcgctttgcc
     3301 gtctttttta tcttccgccc aattttgcca gcgtaaaaaa ttcggcaaaa ttgagtgata
     3361 ttcatcgttt tccagttccc actcttgttc tttagcatcg taaattttca agaataagat
     3421 ccagacgatc tgttcaatgc gttgggcatc accgttaatc cctgcatcgt tacgcattac
     3481 atcttgcagg cgttttacta tgttgttgag gctcattgtg atccttgttt atctgtaaaa
     3541 ttttgttgta aaataaccgc ttgttattcc gaataaatca tcttttctaa gccttttacc
     3601 gctttctggt agagttcttt accgccgaaa tttttaataa tagacactag acctccaaaa
     3661 cggctgaatt gcggtgtctt taaaatttcg gttttagtca gatcttgatt gccggatttg
     3721 acatattcat taagcagcaa ccctaaaact tgctggtttt cgggcgaata ctgctccaca
     3781 tagccacttt gtttcacttt ttctgctcgt tcggttttag tcagtggttt tttgccaaag
     3841 gagatgtggc ttaatacatc aaagtcttca acatcgtggc tgaattggcg tgtttggcga
     3901 agggctgcca gccattttgg atcggtgtaa aatttcgaga aaatttcacc gcttgttgtc
     3961 cattgctgtt ggaactgttc gtaactgccg aactcttttt gcatttcccg ttttgagaac
     4021 agatcaatat cttcagaaac cagctcgccc tgctcattca gatattggac tttctcgtct
     4081 aaaatgcgta atttgagtgt gccgaaatcg acaactaccg ggattttata tggctttagc
     4141 tcggggtctt taatgatgtc cttatcactg ggtttaggaa gcggttgttc gacaagtggc
     4201 ttattttccg gtacatctaa aatcaccacc ggctcaccat caaatgccgg atcagcaaac
     4261 aggcgagtaa cgcctcgaaa atctaaaatg gtgaaaaatt ctttgccgaa atcagggcga
     4321 agacgagtgc ctcgtccgat aatttgttta aattccgtca ttgattgaat gtttgcatca
     4381 agtacaacca gcttacaggt tttgctatct acacctgttg tcatcaattt agaggttacg
     4441 gcaatcacag ggtaatcttt cccttcattt gctacatcac aaaattcttc taaatcaacg
     4501 cctttgcctg attggtcgcc tgtaatttgg cagacatatt tggaattgtg tttcaacata
     4561 tcggcattga ggcgtgaaag ctctgccacc attcgctctg catggttgat gttggcacag
     4621 aagactattg ttttctgcaa gcggtcggtt ttgcgtaaaa attcgcaaat tctctccgca
     4681 accaagcggg tgcgatcatt tagcacaatg ttgtaatcga aatctttgac tgtatagagg
     4741 cgatcttcaa tctcattacc ttgatgatct tttaaacctt tggttggtcg ccagcctttt
     4801 aaatcacagt ccagatcaac acgaatcact cgataagggg caagaaagcc gtcttcaata
     4861 ccttgtttga ggctataggt ataaatcggc tcgccaaaat aggtaatgtt gctggcatca
     4921 tcggtttctt tgggggttgc agtcataccg atatgggtgg cgtccttgaa atacttcaaa
     4981 atagcacgcc agtcagaatc ttcttttgct gaaccacgat gacattcatc gatcacaata
     5041 agatcaaaga aagagggttg gaactgctta aacagttcgt tatcggtttc accgtcaagc
     5101 tgttgataaa gtgcaagata gacttcataa gcactgtcga gctttttatt gctgactttc
     5161 accattttgt ctttaaacgg tttgaagtct tgcatcattg gctgatcgac tagtacattg
     5221 cgatcggcaa gaaataaaat tttctttttc gcaccactgg tttttaaacg atggataatt
     5281 tggaaagccg tataggtttt acccgtgcct gttgccatta ccaataaaat cctctgctga
     5341 ccttttgcta cagcttcaac agtacggtta atcgcaattt cttggtagta acgaggctct
     5401 ttaaaggtgt cagtgctact ggtgtaataa ggctgttgaa taattttcag ctcttcggtg
     5461 gtatattgtt tcgcccaacg ttggtggagt tcgtcgggag taggaaatgc tgacatcgga
     5521 agggttcgtt gtacgccagt gataaaatcg tgttccacaa actcatcacc atttgagcta
     5581 tatgcaaaag ggacatcaag caatcgagcg tatgtcattg cttgctgtaa gccgtgtcgg
     5641 gaagagtgat ggttatcttt ggcctcgacc acggcaattt tttgatttgc ggaagtatag
     5701 agcagataat caacccgttt tgcttcgatg cgtttagttt gcgtcgtttt tggatcaaca
     5761 actacttgcc catctgtaaa tcgtttattg gttttaaatg tgtactcttc gccaatttgt
     5821 ctatccgacc atcctgcatt tctaattgca ggagagatta atcgagagcg aatttcttgc
     5881 tcgttcatct gctttaattc ttgaagtgtg tacatatttt tccttccgat aagagtttct
     5941 tatcggaaga ttatagcaaa cagtttaggt aaaattaact caggatttaa ttgataattc
     6001 tacccaactc ttcccccatt cgggtttgca ttcctgcaat aagtgcaggg ttgagatgta
     6061 ctttaccttt cgggaagagg ttaattacgg ttgagcctaa acggaaagct cccatttctt
     6121 cgcctttttg gagtttgatg gctttttcgc cttcggtttc gtaattccaa actaccactt
     6181 catcagcgcg aggcggatta attacgccag cccaaaccgt actcatactt gcggtaaccg
     6241 ttgcaccgac caaaatctgt agcattggtc cgaatgcggt gtcaaattca caaatcacac
     6301 gctcattgcg agcgaataag tttggaacgt gttcggctaa aaacggattg acggagaaca
     6361 attcgcccgg cacataaatc attttacgca atgttccatc gcacggcata tgtacacgat
     6421 ggtaatcagt tggcgataag taggtggtaa taaatgtgcc gtctttaaat tttgccgcca
     6481 tttcttcatc atttgccaat aaggtttcta gcgtaaaatg atgccctttt gcttgtaata
     6541 aacggttatc cgaaatttcg cctgattcgc tgactttacc ttctgccggt aagcaaagtg
     6601 tatttggatc ttgattaatc gggcgtgcgt tttcttttaa ttcacggata aaaaattcat
     6661 taaaagtagc ataatctgac ggctcggttt ttttagcttc agataaattg actttatact
     6721 gttttgcaaa cagtttgatg ataaggtgag taactacgcc ccatttttgc tctgctaacc
     6781 aacctgccgc acgggtaatt gccagttgtg gcagtaaata gtgcagagcg attttcacac
     6841 gttggaaata ggttggaact ggatattttt ttaattgcat tgcgttttcc ttttatttaa
     6901 aaacgagaat ttgcgtttaa ttgggggcga agtatagcaa atacaatgca aaatagataa
     6961 aaaatcttta aggtgttgaa gtggctacaa agaaaaaaac agataggggc tttgtaggaa
     7021 ttccacttca acaatttggt tgtcttattc taaaataata ttggctcgat ttttttctac
     7081 cgttgcttgc ccaattccgg agacttcagc aagttgctca atgtttttaa aattgccgtt
     7141 tttagtacgg taatcgacaa tcgcttgagc ttttttctgc ccaattccca ctagcttgtc
     7201 ttgcaattca gcagcactag cagtattgat atttaccgca ttagcgttgg tgatttgtgt
     7261 ttgctgtacg ggttgagtga ctgcattttg ttcaacaggc attgtagttg gcggtgtttg
     7321 ggcaaaagcc attgcattta agccaaacaa gaatgttaga ccaagttgtt tgattttctt
     7381 cataggattt ccttattagt tgttgaaagg tcgaaaggaa gattaagagg gaataaaata
     7441 gaactcaacc ggcaaaaata aatttttcga cacagatcga aaaaatagaa aatggctaaa
     7501 cttctagacg tgcatttggt ctgaaattca atgaaagtaa atagatttgc aaaaaattag
     7561 gcaaaaatga ccgctagttg cgtaaattta gttaaataaa ttcaaatagc ggctgaattg
     7621 ctttagaata tcgccatttt tatactcatt aacaacgcta ttatttggaa ttaatatgac
     7681 acaaaataat tttcgcaaag aaccggtttt tggtgaatct tcggttacct ctcaagacgc
     7741 tgaagcaacc aaaacagata cagctcaagc cccttatgtt tcgttgcgtt caaccaaagc
     7801 accggggcat acgtttaccc caattatgaa aagacctgat gctaaagtag aagatcatac
     7861 ggctgatgtc ttttcggcta agcaggcaaa agaattccaa tttactccaa gttcagaagc
     7921 tacagaagca gtagagccgg ataataccat tcattcggtt gaagaaagtg tatcaaccgc
     7981 atcttttaac gcggttgaag cacaagaaaa tgtggaaaaa gaagtggtta aacctgcacc
     8041 cgagagtttc actactgagc gtattattcc aaatgcggca acagctgccg cagcagccac
     8101 tgcggcagct gcagccacta aaaccaaaga gattaaagct aaaatgccac ctaaaacccg
     8161 ccgtttatta ttagtggcat tgttggcgtt agcattattg gtactgtttt tcttgttaaa
     8221 accaagtacg ccggaaactg tcgaagagct acaatctcag caaggtggta gcttgccgat
     8281 tgaattccga ccggttgatg aagctgaagc gaaacgtgcg gaagaacaag ctcgtgcaga
     8341 acaagaagcc gctgaagctg caaaagctca agaacaagct caagccgctc aacaagagtc
     8401 tgtaacagtt gctccagccc aaaccgagca aacaatggct aaaccgtcac aagatagcca
     8461 gtcgcaaaca caatcacaag cagtagttga gcaacctgtt gcggtgaaac cggtggaaaa
     8521 taaaccggtt gttgcacaac cggcgattaa gcctaaaacg gaaaatagcg tggtgttcca
     8581 acctgaaaca acggtaaaac aacagcctaa agcccctgct aaaactgaag tgaaaaccga
     8641 acgtgctaca ctggcagcaa aagaaacggc aaaaacagca gctaaaccag ttgagaaagc
     8701 tccgacaaaa actgaagcgg taaaagcagc accggctgcc gagtcggcaa atgcggtagc
     8761 aaccaaaaca atgactgtgc caaaaggtgt atcactaatg caagtgttcc gtgataacaa
     8821 tttaaatatc tcagacgtta atgcgatgag caaagtcaat aacgtggtga gtaatttaaa
     8881 agtaggcgag cgagtaaccg ttcgtttaga taaaaataac cgtgtggttg aaatgagcat
     8941 tggctcaggc ggcaaattta cccgtcaagc aaacggtagc tatactttta aataaataaa
     9001 atgaagaaaa agagcaacgc tacaaacgtt gctcttttat ttaaaaagga gataaaggat
     9061 gaatttattt tgcccctctt acaaacttat ctacttaacg ctaagctcag cgatgttagt
     9121 tgcttgtagt acagtggtta cccctgttga gcaaattcat caagtacaaa aggaaaaaga
     9181 gtcagtaaag aaagtgacta aactgatagc acaagcacct aaagtgagtt tatatcgttg
     9241 tgagaatgat aagaaggttg aagttgaacg ccagggacga acaaaaaaag cgttaaaaaa
     9301 ggaaatagtc acagtgagtt atcaagggac aacgcatcag ctttcacctt ctgtaacgag
     9361 agatggtaaa aaatacacta atatccgttg gacttggcat gaaacccgta gtggtaaagc
     9421 atttttgtat aacaacacta aaaaaacgtt agcggcaaat tgcataaagc agtaacaagc
     9481 ggacacaaaa agtatcgctt tttgaacgtt ggctttgcca acggcagcga agctgtgaac
     9541 aatccttaca aggattgtga ataaattttt aagagaattt tgcaaatgaa tattttagtt
     9601 ataggaccgt cttgggtggg tgatatgatg atgtcgcacg cactttatca acagcttaaa
     9661 atacagtatc cgaattgcca aattgatgta atggcacccg attggtgttg tccgctttta
     9721 gcccgtatgc ctgaggtgcg taatgcgatt tcaatgccga ttggacacgg ttcatttcgt
     9781 ttgtgtgaac gctaccgact cggaaaatcc ctgagaaatc aatatgatat ggcgattgta
     9841 ttgcctaatt cattaaagtc tgcctttatt ccactgtttg cgaaaattgc gagacgtcgt
     9901 ggttggaaag gggaaagtag atattttttc ttaaatgatc ttcgcagtaa taaaaacgac
     9961 tacccaatga tggtgcaacg ctatgtagcc ttggcgtttg aaaaggctac tgttccaacc
    10021 gcacaacagt tgccgattgc ttatccatac ctcacaactg atagcgaaca aattgagcag
    10081 accaaagcga aatttgaaaa acagttggct tatgcggaaa accgtcctgc catcggcttt
    10141 tgtccagggg ctgaatttgg accggcaaaa cgttggccgc attatcatta tgcaatattg
    10201 gcaaaaatgt tgattgagca aggctattcg gtacggttat ttggctcgaa aaaagatgaa
    10261 gccgccggtg agcaaattcg cttggcatta cctgaaaatt tacaacgcta ttgcctgaat
    10321 ttagccgggc aaaccgattt aaaccaagcg gtagatttga ttgcagattg taatgcagtc
    10381 gtgagcaatg actccggctt aatgcacatt gccgcagcac taaataaacc gctggtcgcc
    10441 ttatatggac cgaccagccc acaatatacg ccgccgttat cccaaaatgc cgagatcatg
    10501 cgcttaattg aaggcggttt gattaaaatc cgcaaaggcg aaggggcaga gggctatcac
    10561 caaagtttaa ttgatattca gcctgaaatg gtgatggaca aattagctga actgttagtt
    10621 taaatggata acaagcggtt gaatttgcca attttttgca aaaaaagacc gcttgtttgt
    10681 ttataaaaga tgaaaatttt aatcgttaaa acctcatcaa tgggagatgt attacatacg
    10741 cttccagcca taaccgatat gcaaaatgct attcccgatt taaaagtaga ttgggtggtg
    10801 gaggagaatt ttaccgaaat tcctgcgtgg cattcagcgg taaataaagt aattccggtg
    10861 gctattcgcc gctggcggaa aaacctttgc caaagacaga cttgggtaga atgccagcag
    10921 tttttaaaac agctaaaaag tgagcagtat gatgcggtaa ttgatgctca agggctgatt
    10981 aaaagtgcgg tgttagtaac tcgccaagcc aaaggcaaaa aatttggcta tgataaaaac
    11041 tctgcccgag aagggttaag tagtctgttt tacgatgaaa cgtttaatat cccctaccaa
    11101 caacacgctg tagaacgcat cagaaaatta tgtgcaaaat cattaggtta tgctttgcca
    11161 accgaacggg gcgattattg cattgcacaa cattttgcaa aaaatcctca aaaaacgacc
    11221 gcttacatta tgctgattca cgccactacc cgagcggata aacattggca agaagcctat
    11281 tgggcggagt tgattcaagc gttggataag cacaatctgg cagtcagatt gccttgggga
    11341 aaccaagccg aaaaagaacg ggcggagcgt ttagcgagtg tggcgaataa tgctaaggta
    11401 ttgccaaaaa tgagcttaac cgatattgct actcaacttg cgggagcagc ggcagtggtg
    11461 tcggtggata ccggcttgag ccatttggcg gctgcgttag ataagccaaa tgtgatttta
    11521 tatggggcaa ccgatccaaa acttatcggg gcttacgggc aaaatcagtt ttatctaaaa
    11581 gcagataaaa tggaaaatat tacctcttca caagtgctag aaaaattact tccattgatt
    11641 aacaaatgat cattgttgtt ctcttactgt gcttgacttt tatccacgtt caatttagaa
    11701 ttcaccgtcc tttcgtgtga aaggatgttt ttaattttat ttttaataat tttaactgga
    11761 gcttttttaa tggcaactat caaccagcta gtacgcaaac cgcgtgtgaa aaaggttgta
    11821 aaaagtaacg ttcctgcatt agaggcttgc ccgcagaaac gtggcgtgtg tactcgtgta
    11881 tacactacta cacctaaaaa accgaactca gcattacgta aagtatgtcg tattcgttta
    11941 acaaatggct ttgaagtaac ttcatacatc ggtggtgaag gtcacaacct tcaagaacac
    12001 agtgttgtat taatccgtgg tggtcgtgtt aaagacttac cgggtgttcg ttaccatact
    12061 gtacgtggtg cacttgactg tgcaggcgtt aaagatcgta aacaaggtcg ttctaaatac
    12121 ggcgttaaac gtcctaaagc ttaatggttc tccgttgagt aaggccaaac gtctaaattt
    12181 taatatttaa accataaact ccagtcaaga tgccttaagg cagattagat gagttttgga
    12241 tatcctgaag atttaatata cggagaaatt tccaatgcca cgtcgtcgta gtattgaacc
    12301 acgcaaaatt cttccagatc cgaagttcgg ttcagaatta cttgcaaaat ttattaacgt
    12361 gttaatggta gatggtaaaa aatctactgc agaaaaaatc gtttacggtg cattagaaac
    12421 tttaacacaa cgttctggta aagaagcgtt agaagctttc gaaatcgcat tagaaaacgt
    12481 acgtccaact gttgaggtta aatctcgccg tgtgggtggt tctacttacc aagttcctgt
    12541 agaagttcgc ccaacccgtc gtaatgcttt agcaatgcgt tggatcgtag aagccgctcg
    12601 taaacgtggt gacaaatcaa tggcattacg tttagctaac gaattatcag atgcagcaga
    12661 caacaaaggt tcagcagtga agaaacgtga agacgttcac cgtatggctg aagctaacaa
    12721 agcgtttgct cacttccgtt ggtaatcgtt taagcaaact tatcaaaggc ttcatctcag
    12781 tttgcggtga agccttaccc ttattaagaa ttttaattta aaccccaaac aaggtaataa
    12841 ataaaaatgg ctcgtacaac ccctatttca ctttaccgta acatcggtat cagtgcgcac
    12901 atcgatgcgg gtaaaacaac tactacagag cgtatcttgt tctatacagg tgtaagtcac
    12961 aaaatcggtg aagttcacga tggtgctgca acaatggact ggatggaaca agaacaagag
    13021 cgtggtatca cgatcacatc tgctgcaaca acagcattct ggtctggtat gtcacaacaa
    13081 tatccacaac accgtatcaa cgttattgat actccgggac acgttgactt tactatcgaa
    13141 gtagaacgtt caatgcgtgt acttgatggt gcggtgatgg tttattgtgc agttggtggt
    13201 gttcaaccac aatctgaaac tgtatggcgt caagctaaca aatataaagt gccacgtatc
    13261 gcgttcgtaa acaaaatgga ccgtacaggt gcaaacttct tacgtgttgt tgagcaaatc
    13321 aaaacgcgtt taggtggtaa ctcagttgca ttacaacttc caatcggtgc agaagacagc
    13381 ttcaaaggtg ttgttgattt aatcaaaatg aaagcaatca actggaacga agctgatcaa
    13441 ggtatgacct tcacttatga agatattccg gcagatatgc ttgaagcttg tgaagaacac
    13501 cgtgcaatgc ttgttgaagc agcagctgaa gcttctgaag aattaatgga aaaattcttc
    13561 tctggtgaag aattaactga agatgaaatc aaagcagcat tacgtcaacg tgtattagca
    13621 ggtgaagtaa tccctgtttg ctgtggttct gcatttaaaa acaaaggtgt acaagcaatg
    13681 cttgatgcgg taatcgatta cttaccggca ccaactgata ttcctgcaat taaaggtatc
    13741 aatgaagatg aaactgaagg tgagcgtcac gcaagcgacg atgagccatt tgcttcatta
    13801 gcattcaaaa ttgcaacaga cccattcgta ggtaacttaa ccttcttccg tgtttactct
    13861 ggtgtaatta actctggtga tacagtttac aactcagtaa aacaaaaacg tgaacgtttt
    13921 ggtcgtatcg tacagatgca cgcaaacaaa cgtgaagaga tcaaagaagt tcgtgcgggc
    13981 gatatcgctg cagcaatcgg tttaaaagat gttggtacag gtgacacatt atgtgcaatc
    14041 gatgcaccaa tcattcttga gcgtatggaa ttccctgagc cggtaatctc tgttgcggtt
    14101 gaaccaaaaa ccaaagctga ccaagagaaa atgggcttag ctttaggtcg tttagcacaa
    14161 gaagaccctt cattccgtgt tcacacagat gaagaatctg gtgaaacgat catttcaggt
    14221 atgggtgagt tacacttaga catcatcgtt gaccgtatga aacgtgagtt taaagtggaa
    14281 gcaaatatcg gtaaaccaca agtatcttac cgtgaaacta tccgtactcg tgttaatgat
    14341 gttgaaggta aacacgcaaa acaatctggt ggtcgtggtc aatatggtca cgttgtgatc
    14401 gacttatacc cattagatgc tgaaggtccg ggttatgagt ttgtaaacga gatcaaaggt
    14461 ggtgtaatcc ctggtgaata cattcctgcg gttgataaag gtatccaaga acagcttaaa
    14521 tctggtccgt tagcaggtta cccggtagta gatttaggtg tgcgtttaca cttcggttca
    14581 taccacgatg ttgactcatc tgaacttgcg tttaaacttg ctgcatcatt agcatttaaa
    14641 gcggcgttcg caaaagcaag cccggtatta cttgagccaa tcatgaaagt ggaagttgaa
    14701 actccaccgg attacgtggg tgatgtaatc ggtgacttaa gccgtcgtcg tgcaatggtt
    14761 aacggtcaag aagcgaacga gttcgttgtt aagatcaatg ctgaagttcc actttcagaa
    14821 atgtttggtt atgcaacaga cttacgttca caaactcaag gtcgtgcatc atactctatg
    14881 gaacctttaa aatatgctga agcaccgaaa aacgttgctg atgcaattat tgaagctcgt
    14941 agaaaataat ttgtttaacc tgagccatag caatagggct atggctcatt ttttaggaag
    15001 atattaaagt gtctaaagaa aaatttgaac gtacaaaacc gcacgttaat gtgggtacaa
    15061 tcggccacgt tgaccatggt aaaacaactt taacagcagc aatcactacc gtattagcaa
    15121 aacacttcgg tggtgcagct cgtgcatttg accaaatcga taacgcgcca gaagaaaaag
    15181 cgcgtggtat caccatcaac acttcacacg ttgagtacga tacagcaact cgtcactacg
    15241 cacacgttga ctgcccggga cacgcggact atgttaaaaa catgattacc ggtgcggcac
    15301 aaatggacgg cgctatctta gtagtagcag caactgacgg tccaatgcca caaactcgtg
    15361 agcacatctt attaggtcgc caagtaggtg taccatacat catcgtattc ttaaacaaat
    15421 gcgacatggt agatgacgaa gagttattag aattagttga gatggaagtg cgtgaacttc
    15481 tttcacaata cgacttccca ggtgatgaca ctccaatcgt acgtggttca gcgttacaag
    15541 cgttaaacgg cgtagcagag tgggaagaga aaatccttga attagcaaac cacttagaca
    15601 catacattcc agagcctgag cgtgcgattg ataaaccatt cttattacca atcgaagacg
    15661 tattctcaat ttcaggtcgt ggtacagtag taacaggtcg tgttgagcgt ggtatcatcc
    15721 gcactggtga tgaagttgaa atcgtaggta tcaaagatac agcgaaaaca accgtaacag
    15781 gtgttgagat gttccgtaaa ttattagacg aaggtcgtgc gggtgagaac gtaggtgcat
    15841 tattacgtgg tactaaacgt gaagaaatcg aacgtggtca agtattagcg aaaccgggtt
    15901 caatcactcc acacacagac ttcgaatcag aagtttacgt attatcaaaa gaagaaggtg
    15961 gtcgtcacac tccattcttc aaaggttacc gtccacagtt ctacttccgt acaacggacg
    16021 taacaggtac aatcgaatta ccggaaggtg tagagatggt aatgccgggc gacaacatca
    16081 aaatgactgt tagcctaatt cacccaatcg cgatggacga aggtttacgt tttgcgattc
    16141 gtgaaggtgg tcgtacagta ggtgcgggcg ttgtagctaa aatcatcaaa taattaagat
    16201 tgtttgataa attgaaaagc taaataaaaa ctagaaagag gcatatcgaa aggtatgcct
    16261 cttttgcttt tatgccgatt aagctagcca tatcatatca tttcatttgc aaaaatcggt
    16321 gaaaaaagac cgcttgtagg caagcccatt gacaagcagt cagattttag ggatttttta
    16381 caaaactgtt tatttgcgaa tgagtttctt ctcgacagtt tcttgataga acgctacaat
    16441 aaccgtctat ttgtatatct tttttatatc cctaaaccga ggtttaagaa tgtcattttc
    16501 aattgaaaca ttagaaggct taaaacgccg tgttactatt aacgtaccag ctgataaaat
    16561 cgaagcagca tacaaagaac aattaaaagg ctatgcgaaa aatgctcgtg tagatggctt
    16621 ccgtaaaggt aaagtgccac actcaattat cgaacaacgt tttggtattg cagcccgtca
    16681 agatgtgtta tctgatgaaa tgcaacgtgc attctttgaa gccgtcattg ctgaaaaaat
    16741 caacttggca ggtcgcccta ctttcacgcc aaacaactac caaccaggtc aagacttcag
    16801 ctttactgcg gaatttgaag tattcccaga agtggaatta aaaggtttag agaatatcga
    16861 agtagaaaaa ccggttgttg aaatcgcaga cgctgattta gacaaaatgg tagatgtatt
    16921 acgcaaacaa caagcgactt ggtcagaaag ccaagaggct gcaaaagccg aatctcgtgt
    16981 aactattgat ttcgttggtt cagtagatgg tgaagaattc gaaggcggta aagcatcaga
    17041 cttcgtgtta tttatggggc aaggtcgtat gatcccgggg tttgaagacg gaatcgtagg
    17101 tcgtaaagcg ggtgagcaat tcgacattga tgtgactttc ccagaagaat accacgctga
    17161 aaacttaaaa ggcaaagcag cgaaatttgc aattaccttg aaaaaagtag aagagatggt
    17221 attaccggaa ttaaccgaag agtttgtgaa aaaattcggt aatgcaaaat cagtggaaga
    17281 attacgcgca gaaattaaga aaaatatgca acgtgaactt aaaaatgcag tcacttcacg
    17341 tgtgaaaact caagtaatca acggcttaat tgctcaaaat gatgttgaag tgccggctgc
    17401 tgcggttgaa gaagaaatca acgtattacg ccaacaagcg gtgcaacgtt ttggtggtaa
    17461 gcctgaaatg gcagctcaat taccggctga attatttgaa gcagaagcta aacgccgtgt
    17521 tcaagtgggc ttattattat cttcaatcat cggttctaac gaattaaaag tagatgaaaa
    17581 acgtgttgaa gagatgatcg cagatatcgc ttcagcttac gaacaaccgg ctgaagtggt
    17641 tgctcactat gcgaaaaacc gtcaattaac tgaaaatatc cgcaatgtgg tattagaaga
    17701 gcaagcggtt gatgcagtgc tagcgaaagc gaaagtgact gaaaaaacaa tgtcatttga
    17761 cgaagtaatg tcacaacaag ctcaataatt tccttataag gtgggctttt gctcaccttt
    17821 tattactttt acaagcggtt aaatttagtg ataaatttgc atcaaaacga ccgcttattt
    17881 tttgatgtta aggaaattga attatggaac aactaacttt tgctcaacaa ttagagcaat
    17941 tttttgccaa tcataccttg atggtgtttg cttgggttgc attattgatt gcggtaattt
    18001 ttagctttta taaaagtgca acgcaaaaat ataaaattgt cgataacgca caggctacac
    18061 agctgattaa taaagaagag gcagtagtgg ttgatttacg ttctgatgaa gagttccgtg
    18121 ccggtcatat tattgatagc attcacgtgt taccgtcaga cattaaagca gctaaaatca
    18181 atcagattga taaatacaaa gctcgcccgg taattgtagt agacactaat gacttcactt
    18241 caggtggtgt tgcggcagat cttgctaaac aaggctttag cagagtttat gtattaaaag
    18301 aagggattgg cggctggcgt gcagcaagcc taccaactat taaaaaacat aaataatcta
    18361 ttattaagga aattttatgt cagaagaaaa tcaagttgca tcaacagaag aagctacaca
    18421 aacccctttt gagcttcaaa ttcaacgcat ttatgtaaaa gatgtttcat ttgaagcacc
    18481 aaatttaccg actattttcc aacaagagtg gaaaccacaa ttaggctttg agttagatac
    18541 tgaaaccaaa gaaattggtg aagatttata cgaagttacc ttacacatta cggtgcaaac
    18601 tacgttagaa gacagcaatg atgtcgcttt catttgtgaa gtaaaacaag caggtgtgtt
    18661 tactattaaa ggtttagaag gcattcaact tgctcactgc ttagcttcac aatgtccgaa
    18721 tgtgctttat ccttatgctc gtgagctaat ctcaagcctt gtgaatcgtg gtacattccc
    18781 ggcattaaat ctctcaccgg taaactttga tgcgttattt atggattatc tccaacgcca
    18841 agaagaaagt gcagaagagc cggctttagc gaactaagca ttttagatag aaaataggta
    18901 tgcaaggcat acctattttt ttacaagcgg ttattttttc tactattttt tgcaattttc
    18961 taaataagcc gaacagagga aatatgagtg aaatctattc tgccccaatc acggtattag
    19021 gtgcaggttc ttatggaaca gctttagcca ttgccctttc tcgaaatggt cacaaaacct
    19081 atttatgggg gcataatccc gacaaaatgg ccgttttggc atcagagcga atgaaccaag
    19141 attttctgcc tgatattgta ttcccggaag ccttagaaat tgtcgcagat cttgctacag
    19201 cggtacaaaa atcgaaagat ctgctgattg tggtgccaag tcatgtattc tctgaagtat
    19261 tggctcaaat caagcctttt ttgtcccaag atcaccgtat aatgtgggca accaaagggc
    19321 tggagcgaga taccgggcgt ttgttgcaaa cggtagctaa ggaaattttg ggggaaattt
    19381 acccgcttgc ggtgctttcc ggtccgactt ttgccaaaga actggcggca ggcttgccaa
    19441 cagcgatttc gcttgcctca actgatcctg tttttgccga tcaaatgcaa aaacggagtc
    19501 actgttccaa agcgttccga gtttacctca acaatgatat tatcggtgtg caactcggtg
    19561 gggctattaa aaatgtgatt gcaattggtg ccggtatttc agacggaatg ggattcgggg
    19621 cgaatgctcg taccgccttg attactcgag ggctggcgga gattagccgt ttgggtgttt
    19681 cgctcggggc tgatccgaaa acctttaccg gaatggcggg gcttggagat ttagtgctga
    19741 cgtgtaccga taaccaatcc cgaaaccgcc gatttggttt ggcgttaggg caaggaaaat
    19801 cggcagaagt cgcgatagcg gaaatcggtc aagtggtaga aggattttat aataccaaag
    19861 aagcctattt gctcgctcaa acacagggcg ttgaaatgcc gattgtagaa caaatttacc
    19921 aaatgttatt ttgtggcaaa caggcttcgg atgtggtgaa aaccttgttg gggcgtgaac
    19981 gaaaaggcga ataaagaaag gagaataaaa tgcagcagag tgaaattaat cagatttggc
    20041 aagcaattcg agatgaagcc aaagagctgg cgaattgtga acctatgtta gcaagttttt
    20101 tccacgctac cattttaaaa caccataatc taggcgatgc gttaagttat attttggcga
    20161 ataagttaga aaacccgatt atgccggcaa tcgccttaaa agaaattatt gaggaagctt
    20221 atcgagcaga gccacaaatt attgctagtg cagcttgtga tattaatgcg gtcagaacta
    20281 gagatcctgc ggtggacaaa tggagtacac ctctgctgta cttaaaaggc ttccacgctt
    20341 tacaaagcta tcgggtgacg cattatcttt ggaatcaagg caggaaagca ttagcggttt
    20401 atttacaaaa tgaaatttct gttgcctttg atgtggatat tcatcctgcg gcaaaggtcg
    20461 gttgcggaat tatgtttgac cacgccaccg gtattgtggt gggtgaaacc tctgtgatcg
    20521 aaaatgatgt ctcgatttta caaggtgtaa cactaggcgg tacagggaaa gaaaatggcg
    20581 accgccaccc gaaaatccgt gaaggcgtga tgattggagc cggtgcaaaa attctcggta
    20641 atattgaaat cggacgctat tctaaaatcg gggcaaattc agtggtgcta caacctgtgc
    20701 ctgatcacgc cacagcagca ggtgtaccgg ctcgcattat cggcaaatcc tctgaacaaa
    20761 aaccggcttt tgatatgaat caatactttg aagatgagca aggtttgttt ggtgatggga
    20821 tttaggtaag agttattcct ttaatttatg atttagacat tgctaagtac aagttgcaat
    20881 taacctttaa tgattttcag aactactata atggtaaagg ggatttaact atgttttctt
    20941 tttatggtaa gaaaacatgg gaaaatggca tttatgtagg gattgatggt agcattgggc
    21001 gtgcgaataa caaattaagc catcaaagcc agacgataaa actagccaga aatgtatatt
    21061 ctttaggatt aacggtaggt aaaacgttgg aagttgcaag ttttaaccta caaccgtcat
    21121 ttggtatgcg ctatcatcat ttaacatctg caaatgccca attagagaac acaaaatttg
    21181 agactgatag ggtagatctt ttggccattc aagctggttt agcattaaat aaaactttta
    21241 tatttaatga attaaaggta aaacctgaat ta
//
LOCUS       DANLXS010000019        21055 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.019, whole genome shotgun sequence.
ACCESSION   DANLXS010000019 DANLXS010000000
VERSION     DANLXS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21055
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.019"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..804
                     /locus_tag="PXD71_000577"
     CDS             <1..804
                     /locus_tag="PXD71_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL6290734.1"
                     /translation="LFEQKSVRSHYDEEKDKWYFSIIDVIAILTESLDPSAYWRKLKQ
                     RLKAEGNETVTNCHGLKMPSADGKMRLTDVADTEQLFRLIQSIPSPKAEPFKQWLAKV
                     GYERLQEINDPSQSLDRARENWQKMGRSEKWIQQRMMGQETRNKLTDYWKSSGVEQPQ
                     DFAFLTNVIHQEWSGLSVQKHKSLKGLKSQNLRDHMSEAELLFTALAELSSRQIAESM
                     QAVGVDENAQAGKQGGNIAKNARQELENKTGKSVVTGENFLPPAKNEEE"
     gene            complement(898..1377)
                     /gene="trmL"
                     /locus_tag="PXD71_000578"
     CDS             complement(898..1377)
                     /gene="trmL"
                     /locus_tag="PXD71_000578"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL6290735.1"
                     /translation="MLDIVLYEPEIPQNSGNIIRLCANCGFRLHMIEPFGFGWDDKKL
                     RRSGLDYHEFVDIQKYKNFEDFLERAKPKRLFALTTRGEQNHSDVKYELGDFLMFGPE
                     SRGIPKPLLAEMPMSQKIRIPMCKDSRSMNLSNSVAVVVYEAWRQFGYQNAVAAQSI"
     gene            complement(1554..3713)
                     /gene="rlmKL"
                     /locus_tag="PXD71_000579"
     CDS             complement(1554..3713)
                     /gene="rlmKL"
                     /locus_tag="PXD71_000579"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDL6290736.1"
                     /translation="MTNQTTYFATAARGFEEILKTELEQICAAECKVAQGGVHFTTTQ
                     KGAYQALLHSRLASRILLPLISTKIFSDSDLYASIVSFNWADLFDPRDTFYIDFSGTN
                     REIRNTQFGAMRVKDGIVDYFERKGFARPTVDKDNPDVRIHVYLDRENVVVSLDLSGD
                     ALHIRGYREETGKAPLRETLAAAIILRSGWKVGTPLVDPMCGSGTLLIEAAQMAANIA
                     PQLHRKHWGFNAWKGHQQAMWKAVLDEAFRNVELGAVGENCNSSLQKMFFGFDLDHRV
                     LTKAKQNAKNAGVDHLIQWQQGDVAALKNPIPEKIGTVVCNPPYGERLGTTPALIALY
                     SVFGQRLKQQFAGWNASIFSGEPELLNCLRLRSARQFKAKNGPLDCVQKNYSISEHNK
                     RSDLDENLQFEQNAQVAPDFANRLTKNIKKIEKWAKQQGIDAYRLYDADLPEYNLAVD
                     RYGDHIVVQEYQAPKNIDEQKARQRLLDAVSATLYVTGVETNKLVLKVRQKQKGTNQY
                     EKLANKGEYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGEMAKGKTFLNLFAYT
                     GSATVHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLSLRHHRLFQADCLQWLAECRE
                     RFELIFVDPPTFSNSKRMENSWDVQRDHIELMKQLKRILTADGTIVFSNNKRGFKMDF
                     DGLAALGLTAENISHKTLPLDFERNPQIHNCWIVKNV"
     gene            3911..4540
                     /locus_tag="PXD71_000580"
     CDS             3911..4540
                     /locus_tag="PXD71_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL6290737.1"
                     /translation="MTAPTQILLIDDHPLMRQGMRQLLELDHQFSVVGEASNGTDGIK
                     LALRLEPDVIILDLNMKGLSGLDTLRSLRAQGIDARIIVLTVSDEQSDVFALMDAGVD
                     GYLLKDTDTPELLDNIRKAAAGEVVLSETVRQHLLNRQPENDPLSCLTDRELDVLQWL
                     ATGMSNKQIAAQLFISEETVKVHIRNLLRKLNVHSRVAATVLYLEQQKG"
     gene            complement(4576..5586)
                     /locus_tag="PXD71_000581"
     CDS             complement(4576..5586)
                     /locus_tag="PXD71_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL6290738.1"
                     /translation="MATMKDIARLAQVSTSTVSHVINNSRFVSEEIRERVQRVVKELN
                     YTPSALARSLKVNETKTIGMLVTATGNPFFAEVMAGVEQYCQQHQYNLIIATTNGDTE
                     RLQHHLQMLMQRKVDGLLLMCGDTRLNADESLNIPLPLVVLDWWFTELNADKIFENSI
                     SGGYLATKTLIEAGHSKIAIITGNLKKSLAKNRLEGYKKALAEANIPLNPNWVFESHF
                     DFAGGVNGMNALLAQSEKPTAVFACSDTIALGAYQAIWQQGLRIPQDISIIGYDDIEL
                     AKYLSPPLSTISQPKAELGRLAVETLLKRIRFKSTDFQTIMLEPELVLRHSIQTINKN
                     AE"
     gene            complement(5600..6529)
                     /gene="rbsK"
                     /locus_tag="PXD71_000582"
     CDS             complement(5600..6529)
                     /gene="rbsK"
                     /locus_tag="PXD71_000582"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL6290739.1"
                     /translation="MKKLTVLGSINADHVISVPYFAKPGETLTGHSCHIAYGGKGANQ
                     AVAAARLGAKVSFIGCIGSDDIGKAMKSAFEQDGIDTRSIKTIPNEMTGIAMIQVAES
                     GENSIVISAGANGHLDETVVAKCAAEITQADYLLMQLETPLPAIVQAAKLAQQNGTKV
                     ILNPAPARALPDELLNLLDMITPNETEAEILTGVKVVDEASAIQAAQVFHQKGIEKVL
                     ITLGSKGVFVSENGVGKIVAGFRVQAVDTTAAGDTFNGALVTAMLEDKPLDQAIRFAH
                     AAAAISVTRKGAQPSIPTRQETLDFLSQQAVSF"
     gene            complement(6650..7528)
                     /gene="rbsB"
                     /locus_tag="PXD71_000583"
     CDS             complement(6650..7528)
                     /gene="rbsB"
                     /locus_tag="PXD71_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDL6290740.1"
                     /translation="MKKLTTIASAVMLSLSVAATASAKETIALAVSTLDNPFFVSLKD
                     GAQKKADELGYKLVVLDSQNDPAKELSNVEDLTVRGAKVLLINPTDSEAVGNAVAIAN
                     RNKIPVITLDRGAAKGEVVSHIASDNVAGGKMAGDFIAQKLGSGAKVIQLEGIAGTSA
                     ARERGEGFKQAIEAHKFNVLASQPADFDRTKGLNVTENLLSSKGDVQAIFAQNDEMAL
                     GALRAVSSAKKEVVIVGFDGTDDGVKAVESGKLAATIAQQPELIGSLGVETADKVLKG
                     EKVEAKIPVDLKVISK"
     gene            complement(7551..8519)
                     /gene="rbsC"
                     /locus_tag="PXD71_000584"
     CDS             complement(7551..8519)
                     /gene="rbsC"
                     /locus_tag="PXD71_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDL6290741.1"
                     /translation="MATQTKQFQLGRFLIEQRSFVALFILIVSVSMINPDFFSVDNIL
                     NILRQTSVNAIIAVGMTFVILIAGIDLSVGSVLALTGAVAATLVGSELPILLVIPLVL
                     LLGTAFGGISGAIVAKGKVQAFIATLVTMTLLRGITMVYTDGRPISTGFSDQADAFAF
                     IGTGYLFGIPVPIWIMAVVFGLAWYILKHTPIGRYIYALGGNESATQLSGINVNKIKI
                     FVFAVSGFLSALAGLIVTSRLSSAQPTAGVSYELDAIAAVVVGGTSLMGGKGRVMGTL
                     VGALIIGFLNNALNLLDISSYYQMIAKALVILAAVLADNYLGTKKV"
     gene            complement(8529..10025)
                     /gene="rbsA"
                     /locus_tag="PXD71_000585"
     CDS             complement(8529..10025)
                     /gene="rbsA"
                     /locus_tag="PXD71_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDL6290742.1"
                     /translation="METLLKMSGIDKEFPGVKALSNACLSVYGGRVMALMGENGAGKS
                     TLMKVLTGIYSKDAGSISYLGKEVTFKGPKDSQEAGISIIHQELNLVGNLTIAENIFL
                     GRESKTAWGAIDWKKMYAEADKLLARLGVKHSSHQLCAELSIGEQQMVEIAKALSFES
                     QVIIMDEPTDALTDTETASLFKVIRELKAENRGIVYISHRLKEIFEICDDVTVLRDGQ
                     FIGERVIADIDEDQLIEMMVGRRLEEQYPHLHQERGELLLEVKNLSGSGVNNVSFQLH
                     KGEIVGISGLMGAGRTELMKVLYGALPKTAGSIELNGKAISNHCPQDGLNNGIVYISE
                     DRKGDGLILGMSVKENMSLTSLDHFSKAGSIRHDAEKLAVDDFIEMFNIKTPSREQQI
                     GLLSGGNQQKVAIAKGLMTRPNVLILDEPTRGVDVGAKKEIYQLINEFKKDGLSILMV
                     SSDMPEVLGMSDRILVMHEGQISAEFSRAEATQEKLLAAAIGKSTGLN"
     gene            complement(10035..10454)
                     /gene="rbsD"
                     /locus_tag="PXD71_000586"
     CDS             complement(10035..10454)
                     /gene="rbsD"
                     /locus_tag="PXD71_000586"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL6290743.1"
                     /translation="MKKTLNLNAQLSHSIATLGHTDSLTVCDAGLPIPNTIERIDLAL
                     TAGVPSFLQTFDVVVSELFVERVLLAEEIKHKNPQVLAALLERLTQLEQTQQNKIQID
                     YVNHEIFKEQTHYSKAVVRSGECSPYANIILYSGVPF"
     gene            10684..10944
                     /locus_tag="PXD71_000587"
     CDS             10684..10944
                     /locus_tag="PXD71_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HDL6290744.1"
                     /translation="MALLITHKCTNCDMCLPECPNEAISVGDDIYIIDPVLCTECVGH
                     YEKPTCQKVCPITNCIIKDPDNIETEEQLWERFVMIHHPDQI"
     gene            complement(10976..11866)
                     /locus_tag="PXD71_000588"
     CDS             complement(10976..11866)
                     /locus_tag="PXD71_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HDL6290745.1"
                     /translation="MAMNFKQVSQSYPNLCVPLSQYCLIEIAGVDAEKYLQGQLTCDV
                     AKISVGEHTLTSHCDPKGKMSALLRLYRAENEKFMAIIHRSLLPEALTQLKKYAVFSK
                     VTFTELDTPLYGMAGEAAFAKLSENMTALRLTTGQPRAIVWGEELETTADEQLWTLMD
                     IQDGIPVLLQQNQFELIPQATNLQALESAISFTKGCYIGQETVARAKYRGANKRALFT
                     LAGNFESEISLPEVASAIEMQLGENWRATGTVLTSATDENTLWVQVVLNKDVEPNTKF
                     RANGVALEMVELPYSLAENE"
     gene            complement(11875..12738)
                     /locus_tag="PXD71_000589"
     CDS             complement(11875..12738)
                     /locus_tag="PXD71_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL6290746.1"
                     /translation="MIYSMTAFAHLEMKKEWGNAVWEIRSVNQRFLETYFRLPEQFRN
                     LEMTLRERLRSALTRGKVECSLRIDLSKTQNSEIALNKEYAAQVVQSLKWLKETANEG
                     EINLVDVLRFPGVVDNESQDLDQIAQDLLAGFEQILADFIAMRAREGENVQALILQRL
                     EAISVEAAKVQAQMPEILQWQKDRLQQRFDELNLQLDPQRLEQEMVLLAQRVDVAEEL
                     DRLQLHVKETTNILKKGGAVGRKLDFMMQELNRESNTLASKSINADVTNSAVELKVLI
                     EQMREQIQNLE"
     gene            13019..13735
                     /gene="rph"
                     /locus_tag="PXD71_000590"
     CDS             13019..13735
                     /gene="rph"
                     /locus_tag="PXD71_000590"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL6290747.1"
                     /translation="MRPNNRANNQVREIKITRNYTRYAEGSVLIEFGETKVLCNATVE
                     ESVPRFLKGQQQGWVTAEYGMLPRATHSRTQREAAKGKQGGRTMEIQRLIARSLRAVV
                     DLKALGERTVTVDCDVIQADGGTRTASITGACVALYDAMNKLVANGTLKTNPMKGLVA
                     AISVGIVAGEAVCDLEYLEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHQELLTLL
                     DLAKQGISQLVEAQRKALAE"
     gene            13959..16258
                     /locus_tag="PXD71_000591"
                     /pseudo
     CDS             13959..16258
                     /locus_tag="PXD71_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(16336..17037)
                     /locus_tag="PXD71_000592"
     CDS             complement(16336..17037)
                     /locus_tag="PXD71_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDL6290748.1"
                     /translation="MDLQNRENFLNKLAQRMGRPRQNVPEPIPVLENNHAITRLTDRP
                     QPQLCQEFIDFARVMMADVVEAKEADLPQAIIDICEKYGGASVIVNNDQRLKDLGITA
                     AIQAKYEDSYVWDFNQAETNFEKARTANIGVVYGEYGLAESGGIVLFSDKEFGRSVSL
                     LPEKSVVVLRKSTVLPRVAQLAQILHEKAQRGERMPSCVNIISGPSATADIELIKVVG
                     VHGPVNKIYLVIDDL"
     gene            complement(17042..18451)
                     /locus_tag="PXD71_000593"
     CDS             complement(17042..18451)
                     /locus_tag="PXD71_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDL6290749.1"
                     /translation="MSYLQTNTLPFKQRVEQQVNNEIVRKALVKAQETIGANRQRMVD
                     ELGNWEEWRDDAKAIRNHVLANLDAYLYQLSEKVTQNGGHVFFAETAEEATDYIKNVA
                     KAKNAKKIVKSKSMVTEEIGMNHVLEAEGIKVVETDLGEYLLQIVGDKPSHIVVPAIH
                     KDRHRIRQEMHDVLGYQGSETPEEMTAFVRQKIREDFLEADIGISGCNFAVPETGSVC
                     LVTNEGNLRMATTVPKTHIAVMGMERIAPTFKEVDVLITMLARSAVGAKLTAYNTWLT
                     GPRLEGETDGPEEFHLVIVDNGRSKILESEFQEVLRCIRCGACLNTCPAYRQIGGHGY
                     GSIYPGPIGSVISPLLGGYEEFKELPYACSLCTACNSVCPVKIPLAQLILKHREKIAD
                     AGLTPMAERLSIFGFNFANSHPTLWKVGVKVGAKVASKLIKNGKAPVNFGALGEWTKA
                     RDLPTAEGESFREWFNNRG"
     gene            complement(18453..19184)
                     /locus_tag="PXD71_000594"
     CDS             complement(18453..19184)
                     /locus_tag="PXD71_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDL6290750.1"
                     /translation="MNVNFYVTCIADVMKAGVAKNTVLLLEKLGCKITFLEKQGCCGQ
                     PAINSGYTKQALAGMKNLVETFEANDYPIVAPAGSCVYAIKTYPDHFERFGEKEWAER
                     AKKVADRFYDLTDFIVNKLGVKDVGAYLPGKAVYHPSCSLFRKLGIKDEPIALLQHVK
                     GLELLPIKNQETCCGFGGTFSVKMAEISGEMVKEKVANVDTDEPDYLIGADVSCLMNI
                     GGRLSREGKPIKVMHIAEVLMQGGK"
     gene            complement(19328..20932)
                     /locus_tag="PXD71_000595"
     CDS             complement(19328..20932)
                     /locus_tag="PXD71_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL6290751.1"
                     /translation="MALFLSVFPIVLLIYLMVKRNALPSYVALPLTALLIFVLQLTYF
                     GNDTTLIFANIIAGLGDVLTPITVIFGAILFNRFSEVSGATNTMRKWLGTINPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILVGLGFKPLQVAMLALVMNSVPVSFGAVGT
                     PTWFGMGPLLKDGLLTDAQVLEIGSITALIHSIAAFIIPVMALRLIVSWKEIRQNIVF
                     IYISIFACVIPYFIIAQFNYEFPSLVAGAIGLLVSVWVANMGIGLAKSENHLDGDKAT
                     FGEVAKALLPTGLLIFILVITRIQQLPLKAMLNDATAWIVSSLGFANFEISQGLIFAL
                     KNILGTNVATSYKLLYVPALIPFVVTVLICLPIFSVSGKNTQEIFGTSFKQVKKPFLA
                     LLGAIVMVKLMLIGGVDSMVQIIGKNFAEFTGQSWTLFASFLGAIGAFFSGSNTVSNL
                     TFGTVQYATAQQVGLSVTLILALQSVGGAMGNMVCINNIVAVCSVTGIEKQEGNIIKK
                     TAVPMFVYGVIAAISALFIVPMFYNL"
BASE COUNT         6134 a         4782 c         4063 g         6076 t
ORIGIN      
        1 ctttttgaac agaaatctgt acgcagtcat tacgatgaag aaaaagataa atggtatttt
       61 tctattattg atgtgattgc tattttaacc gaaagcctag atccctctgc ttattggcgt
      121 aagctaaaac agaggctaaa ggcagaaggc aatgaaaccg tgacaaattg tcacgggttg
      181 aaaatgccct ctgctgacgg aaaaatgcgt ttgactgatg tagcagatac cgaacaactt
      241 ttccgactta ttcaatctat ccctagtccg aaagcagagc ccttcaaaca atggttagca
      301 aaagtaggtt atgaacgctt acaggaaata aacgatccaa gtcaaagtct agatcgagca
      361 agggagaatt ggcagaagat gggacgaagt gaaaaatgga ttcagcaaag aatgatggga
      421 caagaaaccc gaaataaact gaccgattat tggaaatcgt caggggttga acagccacaa
      481 gattttgcat ttttaaccaa tgtgattcat caagaatgga gcggactatc tgtacaaaag
      541 cataaatcgc ttaaaggttt aaaaagccaa aatctgcgag atcatatgag cgaagctgaa
      601 ttgttattta cagcacttgc tgaactttcc agtcggcaaa ttgcagaaag tatgcaggca
      661 gttggggttg atgaaaatgc ccaagcagga aaacaaggtg gcaatattgc caaaaatgcc
      721 cgtcaggaat tggagaataa aacaggtaaa tcagttgtta ccggagagaa ttttttacct
      781 ccagctaaaa atgaagaaga atagacacaa tagatgttct taattagtca aggtcgagcc
      841 tcccccaacc ttgaccacaa acttgcaaaa aatagccaaa atcacaccgc ttgcaagtta
      901 aatagattgt gccgctaccg cattttggta gccgaattgt cgccacgcct catacacaac
      961 caccgccacc gagttagata aattcatact gcggctgtct ttgcacatcg gaatgcggat
     1021 tttttgcgac atcggcattt ccgctaataa tggctttgga atgcctctgc tttccggccc
     1081 gaacattaaa aaatcgccaa gttcatattt cacatcactg tggttctgct cccctctggt
     1141 ggttaaggcg aacaagcgtt taggcttagc acgctcaagg aaatcttcaa aatttttata
     1201 tttttgaata tccacaaatt cgtgataatc cagccccgaa cggcggagct ttttatcgtc
     1261 ccaaccaaag ccgaacggtt caatcatatg caaacgaaag ccacagttgg cacaaaggcg
     1321 gataatattt ccgctgtttt gcggaatttc aggttcgtat aaaacaatat ctaacatttt
     1381 ttagccttac tctctcccct tgtgggagag agaccgaaaa atatttcgtt cagaaatatt
     1441 tttcagagag agggggaaat aactctttca tcagtaactt cttaccctct ccctgatttt
     1501 tcttctgaac gaagaaaaat ctatccctct cccacaaggg tcgagggtat tatctataca
     1561 ttcttcacaa tccaacaatt atggatttgc ggattgcgtt caaaatccaa cggtaaggtt
     1621 ttatgcgaaa tattttccgc cgttaagcct aatgctgcca aaccgtcaaa atccatttta
     1681 aagccacgct tattgttcga gaacacaatg gtaccatcag cggttaaaat acgtttaagc
     1741 tgtttcatta actcaatatg gtctcgttgc acgtcccaac tgttttccat tcgttttgag
     1801 ttggagaaag tcggcggatc gacaaaaatc agctcaaatc gctcacggca ttctgccagc
     1861 cattgcaagc aatcggcttg gaacaaacga tgatggcgaa gtgataaatt attcagctct
     1921 aaattttgct ccgcccagtt caaataggtg ttcgacatat ccactgtagt ggtcgatttt
     1981 gccccattaa gagcagcgtg aaccgtagct gaacccgtgt aggcaaacag attcaggaat
     2041 gttttgcctt ttgccatttc gcccaccatt ttacgggtta agcgatggtc taggaacaaa
     2101 cccgtatcca agtaatcggt taaattcacc cacagtttcg ccccatactc gttcacatag
     2161 aaatactcgc ctttattggc aagtttttcg tactgattcg tgcctttttg tttttggcga
     2221 actttcaaca ctaatttatt agtttcaaca cctgtcacat aaagcgtagc agagactgca
     2281 tctaacaagc gttggcgggc tttttgctca tcaatatttt tcggggcttg gtattcctgc
     2341 acaacaatgt ggtcgccata tctgtccacc gctaaattat attcaggcag atcggcatcg
     2401 tataaacggt aggcatcaat gccttgttgt ttcgcccatt tttcgatctt tttgatattt
     2461 ttggttaagc gatttgcaaa atccggagcg acttgagcat tctgttcaaa ttgtaaattt
     2521 tcatctaaat ctgaccgctt gttatgctcg ctgatggagt aatttttctg cacacaatcc
     2581 aacggaccgt ttttcgcttt gaattgacgg gctgaacgta aacgcaggca atttaacaat
     2641 tcaggctcgc cgctaaaaat tgaggcattc caaccggcaa attgctgttt taggcgttgc
     2701 ccgaacaccg aataaagagc aattaacgcc ggcgttgtgc ctaaacgttc accataaggg
     2761 gggttacaca ccaccgtgcc gattttttcc ggaatcggat tttttaacgc agccacatca
     2821 ccttgttgcc attgaatgag atgatctacc cctgcatttt tcgcattttg ctttgcttta
     2881 gtcagcacac gatgatcgag atcaaaaccg aagaacattt tttgtaggga cgaattgcaa
     2941 ttctccccta ccgcacccaa ttcaacattt cggaatgcct catctaacac ggctttccac
     3001 atcgcttgtt gatgcccttt ccacgcatta aagccccaat gtttacggtg aagttgaggg
     3061 gcaatattcg ctgccatttg tgctgcttca atcagtaacg tgcctgaacc acacatcgga
     3121 tcaaccaatg gcgtgccgac tttccagccg gaacgcaaaa taatggctgc cgccaatgtt
     3181 tcacgcaacg gagctttacc ggtttcttcc cgatagcccc gaatatgtaa ggcatctcca
     3241 cttaaatcaa gcgaaaccac aacattttca cgatccaaat aaacatgaat gcgaacatcc
     3301 ggattatctt tatctactgt tgggcgggca aaacctttgc gttcaaagta atccacaatg
     3361 ccgtctttca ctcgcattgc cccaaattgg gtgttgcgga tttctcggtt agtgccgctg
     3421 aaatcaatgt aaaaggtatc acggggatcg aataaatccg cccagttaaa tgacacgatg
     3481 gaagcatata aatcgctatc actaaaaatt ttggtgctga tgagcggcag taagatccga
     3541 gaggctaaac ggctgtgtaa taatgcctga taagcccctt tttgcgtggt ggtaaaatgc
     3601 acaccgcctt gagcgacttt gcattctgcc gcacaaatct gctccagttc ggtttttagt
     3661 atttcctcaa aaccacgggc tgccgtggca aaataggttg tttgatttgt cattcttttc
     3721 acacttgaaa taaaaattgg cagtaattat agccgaacaa gcggtctttt tcttgcaaag
     3781 ttttgcaatt tcttttttag ttaaaaatta gctaagatca aaaaagcggc atttagtaaa
     3841 tccctaaact tgctattttg aattaataat tgcaaaatta accgattaag agaaaggaaa
     3901 ggagaaataa atgacagctc caactcaaat tttactcatt gacgaccacc cattaatgcg
     3961 tcagggaatg cgtcaactat tggaattaga tcaccaattt agtgtagtgg gtgaagcaag
     4021 caacggcacc gacggtatca aacttgcatt acgacttgag ccggatgtga ttattctgga
     4081 tttaaatatg aaagggcttt cagggctaga tactttacgc tcattgcgcg ctcaaggtat
     4141 agatgccaga atcattgttt taacagtgtc agacgagcaa tccgatgtat ttgcattaat
     4201 ggatgctggt gtggacggct atttattaaa agataccgac accccggaac tactagataa
     4261 tatccgtaaa gcggctgccg gtgaagtggt attaagcgaa accgttcgcc aacatttact
     4321 caaccgccag ccagaaaatg atcctttaag ttgcttaaca gatcgtgaac tggatgtttt
     4381 acaatggctc gcaaccggta tgtcgaacaa acaaattgcg gctcagctct ttatttccga
     4441 agaaaccgta aaagtgcata tccgcaattt actgcgtaaa ttaaacgtgc attcccgagt
     4501 tgccgccacc gtgctttatt tggaacagca aaaaggctaa ttgaaatcgg ataaaaaacg
     4561 ctgaataact tcacattatt cagcgttttt gtttattgtt tggattgaat gccttaaaac
     4621 taattcaggt tcaagcatta tggtttgaaa atcagtggat ttaaaacgaa ttcgttttag
     4681 caaagtttcc accgcaagcc gcccgagttc cgctttgggc tggctaatgg tactcagcgg
     4741 tggggaaagg tattttgcca gttcaatatc gtcatagccg ataatcgaaa tatcttgcgg
     4801 tattcgtaaa ccttgttgcc agattgcttg gtaagcccct agagcaatgg tgtcgctaca
     4861 ggcaaatacc gctgtcggct tttcgctttg ggcaagcaaa gcattcattc cattaacccc
     4921 gccggcaaaa tcaaaatggc tttcgaacac ccagtttgga ttgagtggaa tattcgcctc
     4981 ggcaagtgct tttttgtagc cttctaaacg attttttgcc aatgattttt ttaggtttcc
     5041 agtaataatc gcaatcttag aatgccctgc ttcaattaag gtttttgttg cgagatagcc
     5101 gccggatatt gaattttcaa agattttatc tgcattgagc tcggtaaacc accagtctaa
     5161 caccaccaat ggcagcggaa tattcaagct ctcatcggca ttcaatctgg tatcgccaca
     5221 catcagtaac aagccatcta cttttcgttg catcagcatt tgcaaatggt gttgcaaacg
     5281 ctcggtgtca ccgttggtag tggcaataat caggttatat tgatgttgct ggcaatattg
     5341 ctccaccccc gccatcacct ccgcaaaaaa cggattgccg gttgccgtta ccagcattcc
     5401 gatggtttta gtttcattca ctttgaggct acgagccaat gcagaaggcg tgtaattcag
     5461 ttctttcacc accctctgca ctctttcccg aatttcctcg ctgacaaatc ggctattgtt
     5521 gataacgtga gaaacggtcg aggtggaaac ctgtgcaagc cgtgcaatat ccttcattgt
     5581 tgccatttgc aaaattttcc taaaaactca ccgcttgttg gcttagaaaa tccagtgttt
     5641 cctgacgggt cggaatagaa ggttgagccc ctttgcgagt cacactaatc gctgccgctg
     5701 cgtgagcaaa acgaatggct tgatccagcg gtttatcttc taacattgca gtcactaatg
     5761 ctccgttaaa agtatcccct gcggcagtgg tatccaccgc ttgaacacgg aagcctgcaa
     5821 cgattttgcc tacaccgttt tcgcttacaa ataccccttt tgagccaagt gtaatcaaca
     5881 ctttttcgat gcccttttgg tgaaacactt gtgcggcttg aatcgcactg gcttcatcaa
     5941 caactttcac accggttaat atttccgctt ccgtttcatt tggggtaatc atatccagta
     6001 agtttaacag ttcatcgggt aacgctcgag caggcgcagg gtttaatatt actttcgtgc
     6061 cgttttgctg agccaatttt gctgcttgca caatcgccgg caacggtgtt tctagctgca
     6121 ttagcaaata atccgcctgt gtgatttcgg ctgcacactt tgccacaacc gtttcatcca
     6181 aatgaccgtt cgctccggct gaaatcacaa tactgttctc gcccgattcc gccacttgaa
     6241 tcattgcaat gccggtcatt tcattcggaa tcgttttaat cgagcgagtg tcaatgccat
     6301 cttgctcaaa ggctgatttc attgctttgc cgatgtcatc actgccaata caaccgatga
     6361 aagaaacctt agctcccaaa cgtgctgccg ccaccgcctg atttgcccct ttaccgccat
     6421 aagcaatgtg gcagctatgt ccggtgagcg tttcccccgg tttggcaaaa tagggaacgg
     6481 aaatcacgtg atcagcgtta atgctaccga gaacggtgag ttttttcata ttaagccctc
     6541 aaattaccta gtacaacaaa actaggattc tgatagcact agtctctgac gagtgctatt
     6601 ttattaattc aaagcgttcg tcagagacga acgctatctt caagtataat tattttgaaa
     6661 taacctttaa atcaaccgga atttttgctt ccactttttc gccttttagc actttatcgg
     6721 cagtttctac gcctaatgag ccaatcagtt caggttgttg agcaatcgtt gcggcaagtt
     6781 taccgctttc aacggctttt acgccatcat ctgtgccgtc aaagcccaca attacaacct
     6841 ctttcttcgc tgaactcact gcacgcaaag ctcctagtgc catttcatca ttttgagcaa
     6901 aaatagcttg tacgtcccct ttgcttgaga gcaagttttc agtcacattt aagcctttag
     6961 tgcggtcaaa atcagccggt tgactggcta atacattgaa tttatgtgct tcaatcgctt
     7021 gtttaaagcc ttcaccacgt tcacgagcgg cggaagtgcc ggcaatgcct tctaattgaa
     7081 tcactttcgc ccctgagcct aatttctgag caatgaaatc tcccgccatt ttgccgccgg
     7141 caacgttatc ggaagcgata tggctaacca cttcgccttt tgctgcacca cggtcaagtg
     7201 taataaccgg aattttgttg cgatttgcaa tagccaccgc atttccgacc gcttccgaat
     7261 cggttgggtt aatcaataac acttttgccc cacgcaccgt taagtcttcc acattggaaa
     7321 gctctttagc cgggtcattt tgggaatcaa gcaccactaa tttgtagcct aattcatctg
     7381 cttttttctg tgcaccgtct ttaaggctca caaagaatgg gttatctaaa gtcgatactg
     7441 caagagcgat agtctctttt gccgaagcag tggctgcaac cgataaactt aacataaccg
     7501 ctgatgcaat cgtagttaat tttttcatag aaatctcctt taaagataga ttaaactttt
     7561 ttagtgccta gataattatc agctaataca gctgctaaaa taaccaacgc tttagcaatc
     7621 atctgataat atgaagaaat atccaataaa ttaagggcat tgtttaagaa accgataatt
     7681 aatgccccga caagcgttcc cattacacgc cctttaccgc ccattagact ggttccacca
     7741 acaactaccg ctgcaattgc gtctaactca taggacacac ctgccgttgg ctgagcagaa
     7801 gagagacgtg aagtcacaat taagccggca agagcagata aaaaaccact cacggcaaat
     7861 acgaagatct taattttatt cacattgatg cctgaaagct gggttgccga ttcgttgcca
     7921 cctaaagcgt aaatgtaacg cccaatcggg gtgtgtttca aaatatacca agctaaccca
     7981 aacactaccg ccataatcca aatcggcaca ggaataccaa ataaatagcc tgtcccaata
     8041 aacgcaaagg cgtctgcttg gtcagaaaag ccggtgctaa tcggtctgcc atcggtataa
     8101 accatcgtaa tcccacgcaa taaggtcatt gtgaccaaag tcgcaataaa tgcctgcact
     8161 ttacctttag caacaatcgc cccactaata ccaccaaatg ccgtccctaa taataaaacc
     8221 agcggaatca ccaacaaaat cggcaattca gaaccgacta aggtcgctgc aaccgcaccg
     8281 gttaaggcta aaaccgaacc gaccgataaa tcaatgcctg caattaaaat cacaaaggtc
     8341 attccgaccg caataatcgc attcaccgag gtttggcgta gaatatttaa aatattatcc
     8401 acgctgaaaa aatcagggtt aatcattgaa acactgacaa ttaaaataaa taacgctaca
     8461 aaagaacgtt gctcgattaa aaatctgcca agttgaaact gctttgtttg agttgccata
     8521 aacacacctt aatttaatcc tgtacttttg ccaatagctg ctgccagtag cttttcttgt
     8581 gtcgcttctg ctcgtgaaaa ttctgcactg atttgccctt cgtgcatcac taaaatgcgg
     8641 tcgctcattc ccaacacttc cggcatatcg gacgaaacca tcaaaatact aagcccatct
     8701 tttttaaatt cattaattaa ttgatagatc tcttttttcg cccccacatc aacaccccgt
     8761 gtcggctcgt ctaaaatcag cacattcggg cgggtcatca accctttagc gatcgccact
     8821 ttttgctgat taccaccgga aagcagcccg atttgttgct cccgacttgg ggttttaatg
     8881 ttaaacatct caataaaatc atcgaccgcc aatttttctg catcgtggcg aatactgccg
     8941 gctttggaga aatgatcgag agaggtcaaa gacatatttt cttttaccga cattcccaaa
     9001 atcagaccat cgcctttacg atcttctgaa atatacacaa tgccgttatt caacccatct
     9061 tgcggacaat ggttggaaat cgcttttcca tttaactcta tcgagccggc tgttttcggc
     9121 aatgcaccat aaagcacctt cattaattct gtacgccctg cccccattaa gccggaaata
     9181 cccacaattt cacctttgtg taattggaat gacacattat tcaccccact tccacttaaa
     9241 ttcttcactt caagtaataa ctcgccacgc tcttggtgta aatgcggata ttgctcttcc
     9301 aaacggcgac caaccatcat ttcaattaac tgatcttcat caatatcggc aattactcgc
     9361 tcgccgataa attgcccgtc tcttagcacc gtcacatcat cacaaatttc aaaaatctct
     9421 tttaaacgat gggaaatata aacaatgcct cgattttccg cttttaactc tcgaataact
     9481 ttaaacaacg atgctgtttc tgtatcggtt aaagcatcgg tcggttcgtc cataataatt
     9541 acttgagact caaaactcag ggctttcgca atttccacca tttgctgctc gccaatggat
     9601 aactccgcac aaagctgatg gctactgtgt tttaccccaa gtcgggcaag cagtttatcc
     9661 gcctcggcat acattttttt ccaatcaata gctccccaag cggttttgga ctcacggccc
     9721 aaaaaaatat tttcagcaat ggttaaatta ccgactaaat tcagctcttg gtgaataatg
     9781 ctaataccgg cttcttgtga atctttcggg cctttaaagg taacttcttt gcctaaatag
     9841 ctaatagagc cggcatcttt gctataaatc cccgtcaata ctttcatcaa ggtggattta
     9901 cctgccccat tttcgcccat taatgccatt actcgcccac cgtaaacaga taagcacgca
     9961 ttgctcaagg ctttcacgcc gggaaattct ttatctatcc cgctcatttt taataaggtt
    10021 tccatcattc caccctaaaa cggcacgccc gaatataaaa taatattggc atacggtgaa
    10081 cactcacccg aacgcacaac agctttactg taatgtgtct gctctttaaa gatttcgtgg
    10141 ttcacataat caatttgaat tttattctgt tgagtttgtt ctaattgggt taaacgctcc
    10201 aaaagagctg ctaacacttg cggattttta tgtttaattt cttcagcaag tagcactcgt
    10261 tcaacaaata attcactaac tactacatca aatgtttgta aaaaacttgg cacaccggcg
    10321 gttaaagcca aatcgatccg ctctattgtg tttggaatcg gcaaaccggc atcgcaaacc
    10381 gttaaactat cggtatgccc gagtgtcgca atactgtgcg aaagttgtgc atttagattt
    10441 agcgtttttt tcatactaac ccctttattt tcgctccatc acgttggaaa atactccatc
    10501 gaaacgtttc gatggcatca taaaaaattt tcttaatcgg ataaacaacc aaattgcaaa
    10561 aatgtgaagt agttcaaatt attttgcttt ataataggta gcatttagtg acaagcggta
    10621 agatttgcaa atttttttgc aaaatcgacc gcttgtttaa tttttatttt ctcaatataa
    10681 actatggctt tattaattac ccacaaatgt accaactgtg atatgtgctt gccggaatgc
    10741 ccgaacgagg cgatttcggt gggcgatgat atttatatca tcgatccggt gttatgtacc
    10801 gaatgtgtcg ggcattatga aaagccgact tgccagaaag tttgcccgat taccaactgc
    10861 attatcaaag atcctgataa tatcgaaacc gaagagcagt tgtgggaacg ttttgtgatg
    10921 attcatcacc ctgatcagat ataaaaaaac ggtcataccc tgatgaccgt tcgttttact
    10981 cattttctgc caaactataa ggtaactcga ccatttccaa cgctacacca ttggctctaa
    11041 acttggtgtt aggttcaaca tctttattca gcaccacttg cacccacagg gtattttcat
    11101 cagtcgcact ggtcaacaca gtgccggttg ctcgccaatt ttcgcccagt tgcatttcaa
    11161 tcgcagaagc aacttccggt aagctgattt cgctttcaaa gttgccggca agggtaaata
    11221 aagcacgttt attcgcccca cgatatttcg ctctcgctac cgtttcttgc ccgatgtagc
    11281 aacctttggt gaatgaaatc gcagattcaa gtgcctgtaa attggttgcc tgtggaatca
    11341 gctcaaactg gttttgttgc aagagaacag gaatgccgtc ttgaatatcc attaatgtcc
    11401 agagctgttc atcggcagtc gtttcaagct cttcgcccca aacaatggcc cgaggctgtc
    11461 ctgtggttaa acgcaaagcg gtcatatttt cgcttaattt tgcaaatgct gcttcacctg
    11521 ccataccata aagtggggtg tctaattccg tgaaggtcac ttttgaaaaa accgcatatt
    11581 ttttaagttg cgtcagggct tcaggcaata agctgcggtg aatgatcgcc ataaatttct
    11641 cattttctgc acgatacaaa cgcagtaacg cactcatttt gcctttcgga tcacaatggc
    11701 tggttagcgt atgttcgccc accgaaattt ttgccacatc gcaggttaat tgcccttgaa
    11761 gatatttctc agcatctacc cctgcgattt caattaaaca atactgagaa agcggcacgc
    11821 agagatttgg atagctctgg ctcacttgct tgaaattcat tgccataatc tgtcctattc
    11881 taaattctga atttgttcac gcatctgttc gatcaacact ttcagctcca ccgccgaatt
    11941 ggtcacgtcc gcattaatcg attttgaggc gagcgtgttg gattcacggt ttagttcttg
    12001 catcataaaa tccagtttac gcccaaccgc accgcctttt ttcaggatat tggtggtttc
    12061 tttaacgtgt aattgcaagc ggtctaattc ctccgccaca tcaacacgtt gggcaagtaa
    12121 caccatttct tgttctaagc gttgtggatc aagctgtaaa ttcagttcat cgaaccgttg
    12181 ttgtaaacga tctttctgcc attgcagaat ttccggcatt tgggcttgta cctttgcagc
    12241 ctcaactgaa attgcttcta aacgttgtaa gatcagggct tgcacgttct cgccctctct
    12301 ggctcgcatt gcgataaaat ctgccagaat ttgttcaaag ccggctaata aatcttgagc
    12361 aatttggtct aaatcttggc tttcattatc caccacgccc gggaaacgta gcacatcaac
    12421 aaggttgatt tcgccttcat ttgcggtttc ttttaaccat tttaatgatt gaaccacctg
    12481 tgcggcatat tctttgttta acgcaatttc actgttttgg gttttgctta aatcaatgcg
    12541 taagctacac tctactttgc ctcgtgtcag tgcagaacgt aaacgctcac gcaaggtcat
    12601 ttctaggttg cgaaactgct ccggcaaacg aaaataggtt tctaaaaagc gttgatttac
    12661 cgagcgaatt tcccacaccg cattgcccca ctcttttttc atttctaaat gggcaaatgc
    12721 tgtcatacta taaatcattg gtattccttg tgtttttata agtaaagctc tctccccttg
    12781 agggagagag accgaaaaat atttcgttca gaaatatttt tcagagagag ggaggaattt
    12841 gtaaaatttg agataaaaat aaccgcttac ccctctccct gatttttctt ctgaacgaag
    12901 aaaaatccgt ccctctccca caaggggcga gggctatgta aattctttgc taaagaaaaa
    12961 tcacatttta ctgtgttctt tgctaaaatg gtaaaaattt tagcaaaagg aaaagaaaat
    13021 gcgtccaaat aatcgagcaa acaaccaagt tagagaaatt aaaattaccc gtaactacac
    13081 tcgctatgcc gaaggctcgg tattaattga gtttggcgaa accaaagtat tgtgtaacgc
    13141 tacggtagaa gaaagcgtgc cacgcttttt aaaaggtcag caacaaggtt gggttaccgc
    13201 agaatatggt atgttgccac gagccaccca ctctcgcact cagcgtgaag cggcaaaagg
    13261 caaacaaggc ggacgcacaa tggaaatcca acgcttgatt gcccgttctc tgcgtgcggt
    13321 ggtagattta aaagccttag gcgaacgtac cgttaccgta gattgtgatg ttattcaagc
    13381 cgatggaggc actcgcaccg cctcaattac cggtgcgtgc gtagcattgt acgatgcaat
    13441 gaataagtta gtagctaatg gaacactaaa aactaaccca atgaaaggtt tagtcgcagc
    13501 aatttctgtc ggtattgtag caggcgaagc ggtgtgcgat ttagaatatc tggaagattc
    13561 caacgctgaa actgatatga acgtggtgat ggtcgaagat ggtcgtttag tggaagtaca
    13621 aggcacggca gaaggcgaac cgttctcgca ccaagaatta ctgacattgc tagatttagc
    13681 gaagcaaggc attagccagc tagttgaagc tcaacgtaaa gcgttagctg aataattcac
    13741 ttctcttttt gattttcttt ttaaataaaa tctacatctg acaaaacaga ataaccgttc
    13801 tgattcagat gtagattttt ttatttccct tttcaaaata cactcaatca taaaataaca
    13861 attatttttt tatggaaata ttgcacttct tacaaaatat ctgtaaattt cattgccatt
    13921 atttttttat ggctatttat tctagaagga ctctaaacgt gttaaagaaa aattatctca
    13981 ctgtatcaat tctccttgcc attagtggag taggctacgc taatgaaatc tctttagaga
    14041 ctattactgt tgatggaaat acgccttcaa ccaaagggaa attactaggt ggtgaactaa
    14101 atagcaatga atctgttgtt gatgaaaaaa attaaaacaa ggtagtatta cattaggtaa
    14161 cgctttatct ggtgaattag gcattcattc aagccagttc ggaggcggtg caagtacccc
    14221 aattattcgt gggcaagaga gtaaacgtgc aaaaatctta caaaataatg gcgaaaactt
    14281 agatatgtcg ggaatgtctc ctgatcatgc agtaaccgta gatgcgctac ttgctaaacg
    14341 tattgaaatt ttacgtggtc caactacttt actttacagt gcaggtaata cggcaggtgt
    14401 aattaatgtg gtagacaata aaattccaac cgctattccc gaaaaaggct atgaggggca
    14461 atttggtgtg cgttttggct cagccagtaa agagcgttta acctatgcag gctcaacctt
    14521 tgcccttgga aatcatttag ccttgcgggt acagggaatg tataacaaag cttccgaata
    14581 ctatgctcca cactttacga ttgaaggcaa accttaccac cgcgtaccag atagcgatgt
    14641 acaatcacaa accggcacag taagcctttc ttggattgga gagcgaggac atcttggtat
    14701 tgcttacact gatcgccgtg ataaatatgg attaattggt cacacacaca aatacgatca
    14761 ctatactatt agtattattc gccaagccgt tatgtttgca aaaggttatt tacgttttta
    14821 tccgcacctt gcagaagaag gagatattga ctataacaac cctggtatcc gtttactgca
    14881 tactcatatt ccgggaggct cacattatgg acaagatact cacgagcacg gcaaaccttg
    14941 gattgatatg cactccaagc ggtatgatat tgatggaagt ttgcaaaatc cacttcctgg
    15001 ttttgaagaa gccaaaatat cagctaacta tgtagattac tatcatgatg aaaaagatgg
    15061 aaaaagagtt gaaaattact tcaagaacaa aggaaaaaat ctacgtttcg aattggttca
    15121 caaagagtgg aaagggctaa aaggagctat tggtgtgcaa tataccaacc aatctacctc
    15181 agcacttgca cttgaagctt cacgagctgc aaaagtattt aataaacagc ctttactcaa
    15241 taatccaaaa acaaaattat ggagtctctt tgcgattgaa cgccttaatt taggtgactt
    15301 tacctttgag ttaagtgggc gggcagaacg tcaaaaaata gcaatggatt atgatgtgaa
    15361 attaattgat cgctggctag gctttaatac acctatgcca aacttggacc cacataaaga
    15421 caaaggttat tcttatagct ttgcaactca ttggtatttc gcaccaaacc ataagttaac
    15481 attaaatgct gcacatcaag aacgtttacc taatgctcag gaactctatg ctcatggtaa
    15541 acacattgct ttaaatgcat ttgaggctgg caataaaaat ctaaaaaaag agcgttctaa
    15601 ccaaattgag ctatcattag cttatgttgg cgacaaatgg gattataaat taaatctcta
    15661 ccacacccgc tatggcaact atatttaccc attaactctt aatgataatc gtggacctaa
    15721 atcttttacc gatgaatata acttgaaagt gaatagatat tatcaaggcg aagctcgttt
    15781 tagtggggca gaaggcgaaa ttggttatct atttacacca aattatcgct tagccgtatt
    15841 cggagactat gtccgtggaa agttagtcaa cttacctaat attgcaatga gttacaacat
    15901 ttggacaggt gaagttgaca aatgggcttc tcagccagac atttctgcac ctcgtatacc
    15961 accactacgt ttaggagcaa gatttaatgc tgacttcaac ctaaactggt ctggaatgtt
    16021 agaatattac cgagtatttg ctcagaaaaa agtctcgaaa tacgaacaag ttacccctgg
    16081 ccatcatcaa gtaaaccttg gcgtaaccta tagtaatcat tttaatcaaa cagaatacca
    16141 agtattcctt aaagtggata acttgcttaa tcagaaaatg taccaacatg cctcatatct
    16201 accacatatt ccacaaatgg gtagaaatgc aatgcttgga atgaatatca gtttttaatt
    16261 tgtaaatctc tacaaaaata taaccgcttg taaatttcta tctacaagcg gttactttca
    16321 gtggattttt tgcaattaga gatcgtcaat taccaagtaa attttattta ccgggccgtg
    16381 aacgccaact actttaatca actcaatatc cgcagtcgcc gatgggcctg agatgatatt
    16441 cacacaagat ggcattcttt cacctcgttg ggctttttcg tgcagaattt gtgcaagctg
    16501 tgccacacga ggtaacactg tacttttgcg taacacaacc accgattttt cgggcaataa
    16561 gctcactgaa cgcccaaatt ctttatcgga aaacagtaca ataccgccag actctgccaa
    16621 gccatattca ccgtaaacca caccgatatt agcagttctg gctttttcaa agttggtttc
    16681 agcttggttg aaatcccaca catagctgtc ttcgtatttt gcttgaatgg ctgcggtaat
    16741 cccaagatcc tttaaacgtt gatcgttatt cacgatcaca ctcgccccgc catatttttc
    16801 acaaatatca ataatggctt gtggtaaatc cgcctctttt gcctctacta catctgccat
    16861 catcactcgg gcaaaatcga taaactcttg gcaaagttgt ggttgagggc gatcggttaa
    16921 acgggtaatc gcgtggttgt tttccaacac aggaattggc tcaggcacat tttggcgtgg
    16981 tctgcccata cgctgtgcca atttatttaa gaaattttca cgattttgta aatccatttt
    17041 cttatcctct gttattaaac cattcacgga aactttcgcc ttcggcagtc ggtaaatcac
    17101 gggctttcgt ccattcacct aacgccccaa aattcaccgg tgctttgccg tttttaatca
    17161 atttgcttgc cactttcgcc cccactttta cgccgacttt ccaaagggtt gggtgtgagt
    17221 tagcgaagtt aaagccaaaa atagacaagc gttctgccat tggcgttaag ccggcatccg
    17281 caattttctc acggtgtttt aagataagct gtgccagtgg aattttcact ggacaaacac
    17341 tgttacaagc ggtacaaagt gagcaagcat acggcaattc tttaaactct tcgtagccac
    17401 cgagcaatgg cgaaataact gaaccaatcg ggcccggata gattgaacca tagccgtgac
    17461 cgccaatttg gcgatacgcc gggcaagtgt tcaaacatgc accacagcgg atacaacgca
    17521 acacttcttg gaattcgctt tctaagattt tagaacgacc gttatcgaca atgaccaagt
    17581 ggaactcttc cggaccatcg gtttcgcctt caagtctagg acctgtcagc caagtgttgt
    17641 aagcggtgag ttttgcccct accgcactgc gagccagcat ggtaattaat acatcaacct
    17701 ctttaaaggt tggggcgata cgttccatac ccattactgc aatatgcgtt ttcggcacag
    17761 ttgttgccat acgcagatta ccttcgttgg tcactaaaca aaccgagccg gtttccggca
    17821 ccgcaaagtt acagcctgaa ataccgatgt ctgcctctaa gaaatcttca cggatttttt
    17881 ggcggacgaa tgcagtcatc tcttcaggtg tttccgaacc ttgataacct agcacatcgt
    17941 gcatttcttg gcggattctg tggcgatctt tgtggattgc aggtacaaca atatgagaag
    18001 gtttatcgcc cacaatttgc aataaatatt cgcccaaatc ggtttcaacc actttaatgc
    18061 cttctgcttc taaaacgtgg ttcatcccaa tctcttcggt taccatcgac ttcgatttta
    18121 cgattttctt cgcattttta gctttcgcca cgtttttgat gtaatcagtg gcttcttcgg
    18181 cggtttcggc aaagaaaacg tgtccgccgt tttgggtgac tttttcgctt aattggtaga
    18241 gataagcgtc taaattggct aaaacgtggt tacgaattgc tttcgcatca tcacgccact
    18301 cttcccagtt gcccagctca tccaccatac gctgacggtt tgcaccgatt gtttcctgag
    18361 ccttaactaa ggctttacgc acaatctcgt tattaacttg ctgctcaaca cgttgtttaa
    18421 acggcagggt gttagtttgt aaataagaca tcttattttc ctccctgcat taacacttcg
    18481 gcaatgtgca tcactttgat tggcttacct tcacggctta aacgaccgcc aatgttcatc
    18541 aagcagctta catctgcccc gattaaataa tccggctcat cggtatctac atttgcgact
    18601 ttctctttta ccatctcgcc ggaaatttct gccattttga ccgagaatgt gccaccaaaa
    18661 ccacagcaag tttcttggtt tttgattggt aataactcta accctttaac gtgctgtaat
    18721 aaagcaatag gctcatcttt aatccccagc ttacggaata agctgcaaga cgggtgataa
    18781 actgctttgc ccggtaaata agccccaaca tctttcacgc ccagtttgtt cacaataaaa
    18841 tcggttaaat catagaaacg atcagccact ttcttcgcac gttctgccca ttctttttcg
    18901 ccaaagcgtt caaaatgatc cggataggtt ttgatcgcat atacacaaga acctgccggg
    18961 gcgacaatcg ggtaatcatt agcttcaaag gtttcaacca agtttttcat acctgctaaa
    19021 gcttgcttag tgtaaccgct gttgatcgca ggttgtccac agcaaccttg tttttctaag
    19081 aaagtgattt tacaacctaa tttttccaat aaaagcacag tattttttgc aacccctgct
    19141 ttcattacat ctgcaatgca ggttacgtaa aaattaacgt tcataaagaa ctcccacagt
    19201 aaatataaat aagaattaaa ctcaaaaaat gccgttctaa gacggcgaaa ggaagaggtg
    19261 gatttgcaaa aaatttgcta aatctcaccg cttcctttct aaatcaagta agctgattat
    19321 taactcatta taggttatag aacataggta ctataaacaa tgccgaaatc gcagcaataa
    19381 cgccatacac gaacattggc acggctgttt tcttaataat attgccttct tgtttctcaa
    19441 tgccggtaac agaacacaca gccacgatgt tgttaataca caccatattc cccattgcac
    19501 caccaactga ttgcaatgca agaattaagg taacagataa cccgacttgc tgtgccgttg
    19561 catattgtac tgttccgaag gttaagttag ataccgtatt tgaccctgag aagaacgcac
    19621 caattgcacc taagaacgag gcaaataatg tccaagattg accggtaaat tctgcgaagt
    19681 ttttaccaat gatctgaacc attgagtcta caccaccaat taacattaat tttaccatca
    19741 caatggcacc taaaagtgct aagaatggtt ttttcacttg tttaaatgaa gtgccaaaaa
    19801 tctcttgagt atttttacct gataccgaga aaatcggtaa gcaaattaat acggtcacaa
    19861 caaatggaat caacgcagga acatacagta atttatagct agttgctaca ttcgtaccta
    19921 aaatattttt cagtgcaaaa attaagcctt ggctaatttc aaagttagcg aaaccaagcg
    19981 aactgacaat ccaagctgtt gcatcattta gcattgcttt taatggaagt tgctgaatac
    20041 gggtgattac caaaatgaag atcagtaacc ctgttggtaa taatgctttt gccacttcac
    20101 caaaggtcgc tttatcgccg tcaaggtggt tctcgctttt cgctaaacca atacccatat
    20161 ttgctaccca gactgacacc aataaaccaa tcgccccggc aactaatgat gggaattcgt
    20221 agttaaattg agcaataatg aagtaaggaa tgacacaggc aaaaatgctg atataaataa
    20281 acacgatatt ttgacgaatt tctttccagc tcacaattaa acgcaatgcc ataactggaa
    20341 taataaacgc agcaatagag tggatcagtg cagtaataga accaatttca agcacttgtg
    20401 catcggttag taaaccatct tttaataatg gtcccatacc aaaccaagtt ggtgtgccaa
    20461 ccgcaccaaa tgacaccggc acagagttca ttactaaggc taacattgcc acttgtaacg
    20521 gtttaaagcc taaaccaacc agaattggag ctgcaattgc cgccggcgta ccgaaacctg
    20581 atgcgccttc aatcataaat gcaaatgccc aaccgataat catcaactgt gccaccggat
    20641 tcgggttaat tgtccctaac catttacgca tagtgttggt tgcacctgaa acttcagaga
    20701 aacggttaaa taaaatcgcc ccgaaaataa cggtaattgg ggttaataca tcacctaaac
    20761 cggcaataat attggcaaag attaacgtgg tatcattacc aaaataggta agctgaagca
    20821 caaaaattaa taacgccgta agtggtaaag ccacataaga cggtaacgcg ttacgtttta
    20881 ccattaaata aatgagcaaa acaattggaa atacgctcag gaatagagcc atactgcacc
    20941 tctttatatg attataaaat gacctaactg gtcggatagg tgcgaattgt actcttaaaa
    21001 aaaactaatg taactaggtt gttacaattt tgtgatctag ctcgcatttt taatg
//
LOCUS       DANLXS010000020        20970 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.020, whole genome shotgun sequence.
ACCESSION   DANLXS010000020 DANLXS010000000
VERSION     DANLXS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20970
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.020"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..895)
                     /locus_tag="PXD71_000596"
     CDS             complement(<1..895)
                     /locus_tag="PXD71_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6290752.1"
                     /translation="MSKIEFEKIPNSIRKPSVLTEYNNKDAVTTLPTNEQEVLIVAPM
                     LNGEAAFTAPQKIFSDVEAENLFGKGSVAHLMVRQAIQNNPLIRLTVVGLKDHEAGIA
                     ATGQVSFTGTVTYAGVVRITIAGTAYEVAAAKGEEAQAIVARLVNVINAASYSPVVAS
                     VESETTLKLTSKAKGEISNEITLATRNTATGLTLEARAFDNGQRNALIAPALASVAGT
                     HYNVIISPFSDDENALALRSHLESVSAPIEDKPAIGVMGWRGTYATGTTLTASLNSER
                     IIVGWYKGATESNAMIAAGLGA"
     gene            complement(895..1074)
                     /locus_tag="PXD71_000597"
     CDS             complement(895..1074)
                     /locus_tag="PXD71_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDL6290753.1"
                     /translation="MLKVKAAKGVRVPVENRPHHYIEQEAVEVENTLYYQRRIADGDL
                     IVVKETKSAKETGGK"
     gene            complement(1075..1716)
                     /locus_tag="PXD71_000598"
     CDS             complement(1075..1716)
                     /locus_tag="PXD71_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HDL6290754.1"
                     /translation="MISKIERALIERLTLGLGKMVYAVAGYAGEINDEKLDVLRLPAC
                     LVSYGGSAFEAKSMGVRGKRYQATDVFVVLVITRSMRNQTAGRLGGVTSREVGVNQLL
                     GAVKTLLINQTLDGLVHPIQPKRIRTIWNNAEVKKEKLSAYAIEFEISYNECNVLDDG
                     RFPEGVEPNEQVFKQYQGKLDEPQGDLSGFNNRIFDPNNNAETAFRTEFKDGD"
     gene            complement(1713..2138)
                     /locus_tag="PXD71_000599"
     CDS             complement(1713..2138)
                     /locus_tag="PXD71_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL6290755.1"
                     /translation="MYALVDDFVLRIGEQESIELTDREMVGVVDEATLEVALADSSSQ
                     IDGYLSGRYRLPLKTVPQNLKRICCDLARYHLTSKSLVTMTEEVENRYKLCLKELENI
                     SKGVVSLAVDETNEEESATGENTVQFFNGGNRIWGRDKR"
     gene            complement(2138..2479)
                     /locus_tag="PXD71_000600"
     CDS             complement(2138..2479)
                     /locus_tag="PXD71_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290756.1"
                     /translation="MAKPKKNDNEALNTNENTPLEDKVNEQPEDKTDEQPKPPESETK
                     PDDGALINPIAFEVKLKSIHPQATYGRCGYRFNKETAVEIARKALSDEQIITLANDPY
                     LEFVPVVEGDA"
     gene            complement(2527..3444)
                     /locus_tag="PXD71_000601"
     CDS             complement(2527..3444)
                     /locus_tag="PXD71_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HDL6290757.1"
                     /translation="MFKKSELLKALDTAFRKEFSAGLKVLEPQWGTVAMKVSSSTATN
                     TYAWLGQFPKMQEWVGDRQIKNMQAQGMTIENKLFESTVAVPRTAIEDDQVGLFTPMV
                     KQAAQSAAELPDDLVFSLLKKGKTTLCYDGQNFFDTDHPVYQNVDGTGTSKTQSNITT
                     GSASGKPAFYVLDDSQAIKPLIWQERTTPEIETKFDPSESDHVFMKDQYLWGVRARGN
                     AGFGFWQLIHRVEDSELNSETLEKVLTAMRTLKGDGDKQLNIRPTTLLVPPSLEFAAR
                     KLLEAELIDGTTNTLKGVLKVVVNPFIAE"
     gene            complement(3444..4514)
                     /locus_tag="PXD71_000602"
     CDS             complement(3444..4514)
                     /locus_tag="PXD71_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HDL6290758.1"
                     /translation="MFKHKFKLNPIALSFKLNQEANGRIQLFPFGWFEAQDGRGGRWY
                     VGDENGYALADDINNTAIDLMIDYEHQTLYIAENGKGNPAAGWITKAEYISGEGLFAD
                     VKWTPQATQEIKDGVYRYLSPLFLTDGSGMVIKVLNAALTNRPALHNLQEVVAMSSQF
                     AKFLTPEEENPKMKELLIKLFALSANATDEQITEKLTALEKAKGDSPVALSDVYTELA
                     KEKSQVVALSAKVNHPDPAKFVALSDLQAVQTELNGLKQQINDKERDALIQTALSDGR
                     LLPAQKAWAEKLGKQDLVALSDFLKTVTPNPALASTQSGGEDPNKGTEKVALSAAEIA
                     AAKSLGLSEAAYIEKYKTEESK"
     gene            complement(4773..5189)
                     /locus_tag="PXD71_000603"
     CDS             complement(4773..5189)
                     /locus_tag="PXD71_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL6290759.1"
                     /translation="MIEIQIHGIDEIIATLNRIAAKTANKTDLMRNIAGKMESAVLTN
                     FDEGGRPKWLGIKHRQGTPLVDTENLMGSITADYNNDSAIVGTNEPYAAIHQFGGMAG
                     RGRKVKIEARPFLQLTSQDRQDILDDVQAYWQKLLD"
     gene            complement(5340..6639)
                     /locus_tag="PXD71_000604"
                     /pseudo
     CDS             complement(5340..6639)
                     /locus_tag="PXD71_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(6626..8251)
                     /locus_tag="PXD71_000605"
     CDS             complement(6626..8251)
                     /locus_tag="PXD71_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HDL6290760.1"
                     /translation="MSKKKHNKQIKPNLNPIKNQELQTDLAEITATGRVLSDHPSNFI
                     TPAKMKAIFEDTENGDITAQHELFMDIEERDSSIFANIQTRKRAALGVDWAIVAPRNA
                     TPQEEKLRDEVDELFYQLGNLEDLVMDCMDAVGHGFAALEIEWAFNGKLWYPNAFIHR
                     PQSWFKWDKHDNLLLKTRQNTEGEPLREFGWVVHTHKSRSTQAARNNLFRSLAWLYMF
                     KHYSIHDFAEFLELYGMPIRIGKYGAGATPNEKQTLKRALAEIGHNAAGIMPESMSIE
                     LHNAANAGGAAGNNPFLQMVDWCEKSIARLILGQTLTSGADGKSSTNALGNVHNEVRR
                     DLLVSDVKQLGQTFTQQIILPFLQINFGNIDPTRIPSFEFDTKEPADLALFAESLPKL
                     VDIGMPIPVQWAMDKLGIPEAQENEAVLGRSAPPQAVGLSANFPQKMTACECGCGGKV
                     HALSAQTKTGIKEQDALDDLADEAFSVPDFNAQFDPITKKAVAVVMACSSYDEAAEKL
                     AAAYPDLVSEEHDRYLANALFLADLLGAANAES"
     gene            complement(8320..9945)
                     /gene="terL"
                     /locus_tag="PXD71_000606"
     CDS             complement(8320..9945)
                     /gene="terL"
                     /locus_tag="PXD71_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL6290761.1"
                     /translation="MKIKEFEKELEELRRQLQQNIEASFEGWDDKPQAISERRQKVLD
                     PVHGFEYFVQAYFPHYVRSEHKSQLHHYLFENLPLSVSDLSKSVRQAIAAPRGEAKST
                     ICTQLFPLWCLVRDLKRYIIIAMDTKEQAYSMLEAIKVEIESNPRLRIDFPEVAQGKV
                     WRAGVIMTAKKQKVEAVGAGQKLRGRRHGAYRPDLVVLDDIENDESVETPAQRNKLHN
                     WVLKAVLKLGAAGEKFDVIYVGTILHYDSVLSRILNTKGWRRVRFKAILRFPGNMALW
                     DEWENIYLSEEGDDDTMSDLFYQQHKAEMDKGALVSWLARPILYLMKIRASDGHSAFD
                     SEYQNDPVSGDDAIFANSIQYWTELPDDLIYFGALDPSMGKAGASRDPSAILVGGYHR
                     ESGKLYVVEAQIKKRLPDLIIEDVIRLHSQYNCHRWFVETVQFQEFLQTELVKRSAQR
                     GKPVPATATKPNSDKMLRIESLQPHIANGLILLHRSQSTLESQLRHFPKADHDDGPDA
                     LEMLWRNALSSSAPIEWESVDDFDGEYRSKWRH"
     gene            complement(9949..10095)
                     /locus_tag="PXD71_000607"
     CDS             complement(9949..10095)
                     /locus_tag="PXD71_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290762.1"
                     /translation="MAKKKQTVVHKHYSFRWIVQLACIFGAIKLMLNGTDGWGWLLLI
                     AACM"
     gene            complement(10095..10595)
                     /locus_tag="PXD71_000608"
     CDS             complement(10095..10595)
                     /locus_tag="PXD71_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HDL6290763.1"
                     /translation="MAHDVEVQKAVRQAYVFDRLSLEMAAERAGVSFGTARRWKAAAE
                     KNGDNWEKARDVQAMASGGIENIAQGLLAGFLIKYRTLMTELEENTEMPTAAKVEALA
                     ALADSFAKMTASSKKLLPETSAMATAMRAIEMMANIVKTKKPHLLPDFLEMLDELEVQ
                     FKKEFK"
     gene            complement(10603..10857)
                     /locus_tag="PXD71_000609"
     CDS             complement(10603..10857)
                     /locus_tag="PXD71_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290764.1"
                     /translation="MSRKKQKQTDSEKLDLILGGLNELNDKVDKQNEEIERLHREIQA
                     TNRLVNEMARKNRRDAVVAGGLGGGIVAVGFELLRLKFGM"
     gene            complement(10857..11114)
                     /locus_tag="PXD71_000610"
     CDS             complement(10857..11114)
                     /locus_tag="PXD71_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290765.1"
                     /translation="MIDEKLFQMLISFVVAPLVAFVGKVIFDRINRNEQTVKELKAEM
                     ESKYQSKELAHEVNRGIKEKLDDILDSLKEVNQKLDKKADK"
     gene            complement(11107..11262)
                     /locus_tag="PXD71_000611"
     CDS             complement(11107..11262)
                     /locus_tag="PXD71_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290766.1"
                     /translation="MKLIKKLRQRWQQWRFYRRRPDLAELHNVVRAAVAQGNQHPVGR
                     AKRGRYD"
     gene            complement(11277..11636)
                     /locus_tag="PXD71_000612"
     CDS             complement(11277..11636)
                     /locus_tag="PXD71_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL6290767.1"
                     /translation="MITQQIAGVVGVVSTILAYAFYKSWQLKQERKLTATLKAEKQQQ
                     AVEIQQKNAEVKNAKIQQKHVSETRRIQPSAIDEQLHQHGYFRDDNGLHGVRSDLPEP
                     CGHNGNETPSACTQSDL"
     gene            complement(11633..11878)
                     /locus_tag="PXD71_000613"
     CDS             complement(11633..11878)
                     /locus_tag="PXD71_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL6290768.1"
                     /translation="MKQFLLDLITNKDGTASTTGFIQFMSWLVLSGILIHAYLYDKPF
                     ISDWWFAYAGICVLGSPATKGVVSALKQKERSREAEE"
     gene            complement(11881..12414)
                     /locus_tag="PXD71_000614"
     CDS             complement(11881..12414)
                     /locus_tag="PXD71_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL6290769.1"
                     /translation="MTEPIKNKATIADFNLAFDRLIGHEGGYVNDPKDPGGETNWGIT
                     IHTARANGYTGSMRAMSRADAKTIYLKAFWQRYNCEQFPPELAFQFLDACVNHGSGNA
                     SRMLQRAVGVADDGIIGKVTLAAIERYSVADVGLLFQAERLEFYTKLKHFDRFGRGWI
                     NRMAGNLRYFAKDTVEE"
     gene            complement(12500..12925)
                     /locus_tag="PXD71_000615"
     CDS             complement(12500..12925)
                     /locus_tag="PXD71_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HDL6290770.1"
                     /translation="MSEFESVEHYLPETVKEIVGVIGLPATEKLIKAFGGFSFQFSNG
                     KLYFNKLKEVLGQDDAVKLQAYMGACEVYLPRCETALRMLRNQQIYADYCQLTEQGGL
                     SGRLAIMQICPKYSVCDRVAWEAVRYYQRKHTVSQATLF"
     gene            complement(12996..13211)
                     /locus_tag="PXD71_000616"
     CDS             complement(12996..13211)
                     /locus_tag="PXD71_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6290771.1"
                     /translation="MIENPEFGYTPSNLKMLRKHHNLTQTEVANILGVGQRIVMRWET
                     ELSSTAARSDMPYTKWAELLKNLQKNF"
     gene            complement(13230..13622)
                     /locus_tag="PXD71_000617"
     CDS             complement(13230..13622)
                     /locus_tag="PXD71_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290772.1"
                     /translation="MTDYQKLLNALLNDADEDCFAVYGLRATTDVHQVGDELGNSFVW
                     VDGEKTDEELDGICTMGIQNADEAGLLNAIKNLGRDACKKFDVEFKGFQSYCGQNFIL
                     VKGDSARGGEDKGESIIRNPIVVAVFNS"
     gene            complement(13748..14095)
                     /locus_tag="PXD71_000618"
     CDS             complement(13748..14095)
                     /locus_tag="PXD71_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290773.1"
                     /translation="MKELLLTTLCLLSLPAIAMTEKAEQETANALVSGDYQQLRNVAY
                     GMETGSFGHDHNPIAACALRRVILLVNSDKVDMTDFNNEAIACRKIEVTDNQQAWETA
                     FTIAKSISATKKK"
     gene            complement(14184..14609)
                     /locus_tag="PXD71_000619"
     CDS             complement(14184..14609)
                     /locus_tag="PXD71_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDL6290774.1"
                     /translation="MQPQTRKQMIQKIHIGKNELKLDDNAYKMLLLEAVDKPSCSMMT
                     DSELMTVLQTMKAKGFVVKSKKYGKRPTASKTAPHRQGLINKIGALLYQSQKPWDYAH
                     AIAKRSFGIERVQWLTDDQLHKLVQILASYNHRHGMSVK"
     gene            complement(14593..15147)
                     /locus_tag="PXD71_000620"
     CDS             complement(14593..15147)
                     /locus_tag="PXD71_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290775.1"
                     /translation="MKLCKCPICHSDISFEALIEDDAGRELLNTIVNIGGNCGRAVVA
                     YLGLFKPQKNSLANSRALKIVQSLLELYPPSNVLEKALMDTVEQVRRNRRETGRCEPL
                     ANHNYLKKVYESVKPQFAVVRSEAKAVSSLEQQQRQADQKKHDAVMYVERMKQMGQLE
                     AVKNTESYQIWLKWQEEKQNATAN"
     gene            complement(15147..15317)
                     /locus_tag="PXD71_000621"
     CDS             complement(15147..15317)
                     /locus_tag="PXD71_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290776.1"
                     /translation="MEYQKIPEYRLCKKKSGEIVLQQLVICELTPATWHNPYPDSRTY
                     WVDLETIEEGTE"
     gene            complement(15388..15963)
                     /locus_tag="PXD71_000622"
     CDS             complement(15388..15963)
                     /locus_tag="PXD71_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HDL6290777.1"
                     /translation="MKFRFFKGDITQEPLKSIYADYQVSKEERKDKLNAILEKYPFND
                     GLISRSGWFHRIVIGIACKATNISQVLDVKGFKVGKFNDEFYLVKPDKRYEKGKELAE
                     DFKAINEIYEKHRDFSPYILKRLNMLYFVSDFSYSPTSGRSYLAVAGICENTLLVEMP
                     MPIDKNDEPFPEIAEGLVEIKESEFLAIQGK"
     gene            complement(15986..16168)
                     /locus_tag="PXD71_000623"
     CDS             complement(15986..16168)
                     /locus_tag="PXD71_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290778.1"
                     /translation="MTEQEKNELNSQLNEALMQIIQAQKYLKQSDFIRSGVYLGTVQD
                     LLPKVHLKLLTANRKH"
     gene            complement(16235..16381)
                     /locus_tag="PXD71_000624"
     CDS             complement(16235..16381)
                     /locus_tag="PXD71_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL6290779.1"
                     /translation="MKTEQKATEFDRYKHYAEKAAEAERKGNYAEAQDHWKVAKLSAK
                     KTAN"
     gene            complement(16492..16698)
                     /locus_tag="PXD71_000625"
     CDS             complement(16492..16698)
                     /locus_tag="PXD71_000625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290780.1"
                     /translation="MFITFNVNYTDKPVVVNTDKVCSIENINGNVTVHFCDNKKLIMM
                     DFDDKEYASLLNHLHILNQLKRKS"
     gene            complement(16700..17317)
                     /locus_tag="PXD71_000626"
     CDS             complement(16700..17317)
                     /locus_tag="PXD71_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HDL6290781.1"
                     /translation="MSKVTIGNEIYWKDADGNLKPEALVKEIDKERDELVRQFVSKAQ
                     ALSKALGDFKQSVFDDVGAFVSLSADKYGVKIGGAKGNITLFTYDGEYKLQLAVQENI
                     RFDERIHAAKALIDECLHDWSEGAKPELKALIDNAFEVDKEGNLSTAKILSLRRVEID
                     DARWNQAMTAISDSVQVIGSKGYIRFYKRDENGKYQPISLDMAGV"
     gene            complement(17330..17521)
                     /locus_tag="PXD71_000627"
     CDS             complement(17330..17521)
                     /locus_tag="PXD71_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290782.1"
                     /translation="MKKRRMIYALYVGEENVADGTAEELAERLGVEAKTVRFMSSPAH
                     NKRVKYNGKAIRTVKVGLE"
     gene            complement(17524..17844)
                     /locus_tag="PXD71_000628"
     CDS             complement(17524..17844)
                     /locus_tag="PXD71_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290783.1"
                     /translation="MQKVYSEKAGEMMNPLNAFIHDSLVKLEIATLECEAMELEVERV
                     EFEAKSPPRLVLRDNCKTQDLVKNGLASLFGMKTKGGRRYDSYQMQVRGVKCVWESER
                     VTKV"
     gene            complement(17855..18736)
                     /locus_tag="PXD71_000629"
     CDS             complement(17855..18736)
                     /locus_tag="PXD71_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6290784.1"
                     /translation="MKNQELRALMDSKGYQQKQVAQLLGVSVATVSLYLKGDYNGNVA
                     EMDRKVDELIERDKAKVVEAKYNAAFVPTLAARRGMEVMQFAHIEGEINVIFGAAGLG
                     KTQMLKEYERRNSSATLIEVDPSCTPKVLLRKIAEAVGANARGVNNELLESIVNKLKG
                     SERLLMIDEAELLSTRSLEFIRRIHDLTSVGVVLAGMPRLLVNLKGKNNELAQLYSRV
                     GFACDLGNALSEEDLGLLAESALGTNEFNAPLIKACHGNARRLSKLMRGVVRSSEINQ
                     TPISKDLVEQYSKMLIS"
     gene            complement(18740..20677)
                     /locus_tag="PXD71_000630"
     CDS             complement(18740..20677)
                     /locus_tag="PXD71_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6290785.1"
                     /translation="MKDWFSAKELAGISGLSKYPTNVTRQAKKEQWATRPLKGVKGGG
                     FEYHINSLPTEVQTEIRSRFMSAVVEAKPKKLPVVRAEVDVGNLTTKQREIADARMAL
                     VAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLAELVAVANAKKTAKRTLSVRTLNGW
                     VVDYCKAENAEQRLKLLAPQVRQETKPEEIWWLSAFLGVYRQKNGICLTEAYREFEVE
                     WAYQYADNPLLLAQCPSLSQVQRAMNKLPLYVKEYGRRTGSHYKQLLSYVKRDWSVLR
                     ANDVWIGDGHSLKLKVAHPIHGKPFTPELTMIVDGAGRKVVGWSLALSENAFAVADAL
                     RHAISLHGVPAIYYSDNGGGEKNKFLDAEITGMLPRLGIRHETGIAGNPQGRGIIERL
                     NKTIGMTIARQFETGYATGADPETVRKTLYAVNSLANAKGELTPLQRKAQGKLPTWQQ
                     LIEVIQSVIDWYNNEHIHSEIRTTPARKYQQMLHAEDVVMLSPIELRDMSRPEFIRKP
                     DRGWVSWNNNHYFNLKLLDFDREEVVIGIDIHNAESVQVRTKDGRFICEAIWNGNTRE
                     AFPVAMVEQQRKERHKRRANLKQQQLDEINAELNPVLTIEQKNDFSLLATARKPKKEV
                     EPIFLTRAEKEDYEKKLAVGG"
     gene            complement(20692..20919)
                     /locus_tag="PXD71_000631"
     CDS             complement(20692..20919)
                     /locus_tag="PXD71_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6290786.1"
                     /translation="MNKKKRAQDMHRADIRAELMKKGITFSQLGIENGLSKTTVRNAL
                     DKPYKNGEEIIAKALGKTPAEIWPSRYPDVA"
BASE COUNT         5385 a         5256 c         3903 g         6426 t
ORIGIN      
        1 ccgcaccaag ccctgccgca atcattgcat ttgattcggt tgctccctta taccagccta
       61 caataattcg ctcactgtta aggcttgcgg ttaaggttgt gccggtggca taagtgccac
      121 gccagcccat tacaccaata gccggtttat cttcaatcgg ggcagaaacc gattctaaat
      181 ggctacgcaa ggcaagggca ttttcatcat ctgaaaacgg cgaaataatc acgttataat
      241 gcgtaccggc aacactcgcc aacgccggag caattagggc attacgctga ccgttatcaa
      301 acgcacgagc ctcaagggtt aaacctgttg ctgtattgcg tgttgcaagc gtgatttcat
      361 tgctgatttc gccttttgct tttgaggtca gtttaagtgt cgtttcactt tccacacttg
      421 ccaccacagg gctataactc gccgcattaa tcacattaac taaacgagcc acaatcgcct
      481 gtgcttcttc gccttttgcg gctgcaactt cgtaagctgt gccggcaatc gtaatacgca
      541 ccacaccggc ataagttact gtgccggtaa aactaacttg ccctgttgct gcaatacctg
      601 cctcgtggtc ttttaagccc accaccgtta aacggattag cggattattt tggattgctt
      661 ggcgaaccat taaatgagct accgagcctt taccaaataa attttccgcc tccacatcgc
      721 taaaaatctt ttgcggagca gtaaaagcag cctcaccgtt tagcattggt gcaacaatta
      781 gcacctcttg ctcattggtc ggcaaggtgg ttactgcatc tttgttgttg tattcggtca
      841 gcacacttgg tttgcgaata ctgttcggga ttttctcaaa ttcaattttt gacattattt
      901 gccccctgtt tctttagcgg atttggtttc tttgacgaca atcaaatcgc catccgcaat
      961 gcggcgttga tagtaaaggg tgttttcaac ctctactgcc tcttgttcaa tatagtgatg
     1021 cggtcggttt tctaccggca cacgcacccc tttagcggct ttcactttta acatctaatc
     1081 accatcctta aattctgttc taaatgccgt ttcggcattg ttattcgggt caaaaatccg
     1141 attattaaag cccgataaat cgccttgcgg ttcatctagc ttgccttgat actgcttaaa
     1201 gacttgctca ttcggctcaa ccccttcggg gaatctacca tcatcaagca cattgcactc
     1261 gttgtagctg atttcaaact caatagcata agcagagagt ttctctttct tcacttccgc
     1321 attattccaa atcgtgcgga ttcgcttagg ctggataggg tggactaaac cgtctaaagt
     1381 ctggttaatc aataaggttt tcaccgctcc cagcaactga ttaaccccca cctcacgact
     1441 tgttacccca cctaaacgcc cagcagtctg atttcgcatt gagcgagtaa taaccaacac
     1501 cacaaacaca tcagtggctt gatagcgttt acctcttact cccattgatt tcgcctcgaa
     1561 cgccgagcca ccataagaga caagacaagc aggcaaacgg agaacatcta acttctcatc
     1621 gttaatttcc ccggcatacc cagcaacggc atacaccatt ttgcctaagc ccagcgttaa
     1681 acgctcaatc aaagcacgct caattttgct aatcatcgct tatcacgccc ccaaatacgg
     1741 ttgcccccgt taaaaaactg cactgtattt tcgccagtag ccgattcttc ctcattggtt
     1801 tcatcaaccg ctaacgacac tacgccctta gagatgtttt ccagctcttt taagcaaagt
     1861 ttgtagcggt tttccacctc ttcggtcatc gttactaatg atttgctggt taaatgataa
     1921 cgagccaaat cacagcaaat acgctttaaa ttttgcggta cagtctttaa tggcagacgg
     1981 taacgcccac ttaaatagcc atcaatttgg ctggaagagt ccgctaaagc tacttcaaga
     2041 gtcgcttcat caaccacgcc taccatttca cggtcggtta gctcaatgga ttcctgttcg
     2101 ccaattcgca aaacgaaatc atcaaccaac gcatacatta cgcatcgccc tcaacaacag
     2161 gtacaaactc caaataaggg tcgttggcta aggtaataat ttgttcatca ctcaaggctt
     2221 tacgagcaat ttctaccgcc gtttctttat taaaacggta gccacaacgc ccataggtgg
     2281 cttgtgggtg gatagatttg agcttaactt caaaagcaat aggatttatc aaagcaccgt
     2341 catcaggctt agtttcactt tcaggcggtt taggttgttc atccgtttta tcttcaggct
     2401 gttcattcac cttatcttca agcggtgtgt tttcgtttgt gttcaaagct tcattgtcat
     2461 ttttctttgg ttttgccatt ttgctaccct ctcaaaatgg gcgtattgct acgcccaaca
     2521 ttaaacttat tcagcaataa atggattcac taccactttt aacacacctt ttaaggtgtt
     2581 ggttgtgccg tcaattaatt ctgcttcgag taatttgcgt gcagcaaact caagcgaagg
     2641 tggcacaagc aaagtggtcg ggcgaatatt taactgctta tcgccatcgc cttttaaggt
     2701 gcgcattgca gttaatactt tttcaagggt ttcactattt aactcactat cttcaacacg
     2761 gtgaattaac tgccagaaac caaaaccggc attaccacga gcacgcacac cccataaata
     2821 ttggtctttc ataaagacgt ggtctgactc acttggatca aacttcgttt caatttcagg
     2881 tgtagtacgt tcttgccaaa ttaacggctt aatggcttgg ctatcatcta gcacatagaa
     2941 cgcaggctta ccgctggcag agcctgtggt gatattactt tgtgttttac ttgtgccagt
     3001 tccatcaaca ttctgataaa cagggtgatc ggtatcaaag aagttctgcc cgtcatagca
     3061 aagggttgtc ttaccttttt tcaacaaact aaataccaaa tcatcaggta attcagccgc
     3121 actttgtgcc gcttgtttca ccattggggt aaataagccc acttggtcat cttcaatggc
     3181 agtacgaggc acagcaacgg ttgattcaaa taatttattc tcaatcgtca ttccttgtgc
     3241 ttgcatattt ttaatttgac ggtcgcccac ccactcttgc attttcggga attgtcccag
     3301 ccaagcatag gtattagtag cggtgctgga gcttaccttc attgcaaccg ttccccattg
     3361 tggttcaagc acttttaaac cagcactaaa ttctttacga aatgcagtat caagtgcttt
     3421 taataattca gattttttaa acattacttg gattcctctg ttttatattt ttcaatgtaa
     3481 gctgcttcgc ttaaaccaag cgattttgct gctgcaattt ccgcagcaga taacgccact
     3541 ttctccgtac ctttgttcgg gtcttcaccc cccgactgcg tacttgctaa agcggggtta
     3601 ggtgttaccg ttttcaaaaa gtcggataaa gccactaaat cttgcttacc gagcttttcc
     3661 gcccacgctt tttgagccgg caataaccga ccgtcagaaa gagcggtttg aattaacgca
     3721 tcacgctctt tatcgttgat ttgctgtttt aagccgttca gctcggtttg cactgcttgc
     3781 aagtcgctta acgccacaaa cttcgccggg tcagggtgat tcactttggc agagagtgca
     3841 accacttggc ttttctcttt tgccagctcg gtataaacat cacttaacgc caccgggctg
     3901 tcgcctttgg ctttctcaag tgcggtcagt ttttcggtaa tctgctcatc ggtcgcattt
     3961 gccgacaatg caaataattt aattaagagt tctttcattt ttggattttc ctcttcgggg
     4021 gttaaaaatt tggcaaattg gctagacatc gcaaccacct cttgcagatt atgcaaggca
     4081 gggcggttag ttaaggcggc attaagtacc ttaattacca ttccgctgcc gtctgtcaaa
     4141 aacagtggcg agagatagcg ataaacgccg tctttaatct cttgggtcgc ttgtggtgtc
     4201 cacttaacat ccgcaaacaa gccttcgccc gagatatatt ccgccttggt aatccaaccc
     4261 gcagcagggt tgcctttgcc gttctcggcg atgtacaggg tctgatgctc ataatcaatc
     4321 atcagatcaa tcgcagtgtt gttaatatca tcggctaacg catagccatt ctcgtcacct
     4381 acataccaac gtcctccacg cccgtcttga gcctcaaacc aaccaaaagg gaaaagttga
     4441 atacgtccat ttgcctcttg gttgagttta aaactcaatg caatggggtt tagtttgaat
     4501 ttatgcttaa acacaaaaac acctcaaaac aaattaatga ggctaaagaa tagaagattg
     4561 agaaaaaaga gtaacgggga gcaacttcca caagttgcaa attttcagaa aaaaacaacc
     4621 gcttgtattc agcgaggaat gtaacccatt ttaaaacgct ttaaatccgt tttaaaaaat
     4681 tttaaaaaag ttggaatgat aaatcaccat aaaaaaaaca aaaacgccac agagagcgat
     4741 ttaggggcta tctgtggcga ttatcttatc gcttaatcca gcagtttttg ccagtaggct
     4801 tgcacatcgt ccaaaatatc ttgcctgtct tgcgaggtta attgtaaaaa aggtcgggct
     4861 tcaattttta ctttacgccc acgcccagcc ataccaccga attgatgaat agcagcataa
     4921 ggctcatttg tgccaacaat cgccgaatca ttattataat cagcagtaat gctgcccatt
     4981 aaattttccg tatcaactaa cggcgtgcct tgccgatgtt ttatacccag ccatttcggg
     5041 cgaccgccct catcaaaatt ggtcagtacc gccgattcca ttttgccggc aatattccgc
     5101 atcaagtctg ttttatttgc cgttttagca gcaatacggt taagcgtggc aataatttca
     5161 tcaatgccgt ggatttggat ttcaatcatt tttaaccttg caagttgatt tttaaaataa
     5221 acaggggtat attcctttta cggttattag aaaagcggtg ataatctccc tgccgcagcc
     5281 tatttgagat ggcaagtcct gctcaaatga ccgagagcgt gtgtaagtga ccgggagcct
     5341 tacctaataa ccgtattttt caacagcagc ttatccaatt ctttttgtcc aatctccctt
     5401 gttgatacca gaaataattc accaaactct tctaagtatt tcaccacaac aagaatacga
     5461 taatgctcaa agtttcgtat aaagctgaaa tttttgcctt cttgcaaaat atgattaggc
     5521 tgaaaaatta catcaggaat atttgcatac acatcaaacc caaaatcctg cccctcacgg
     5581 ctataaaact gcttaatcag tgtgtcatcc gaaagccata ccgtgccgac tttactgtcg
     5641 attaagcgtt gtgtttcctc acttaaccga ccagcagcaa atttaaagtt ttgattaagg
     5701 gcatttcgca ctttaaccat ctcatcaggc gagagttttt tcgccccatc tttggcaagt
     5761 gccacagcct cttctaactt gttaaacgcc tgcttaaatt caccaccgct catttctgct
     5821 ttggtaaatt ggtaagccag cttttcaggg tataaatcca aattcggctt ataggttaaa
     5881 cgccccacat tataatcaaa acctttatca gccaatcgca ccgtaccatc agccaattta
     5941 aagccaatgg tttcctcttg gttgcctaac ttatctttcg gtcgttttgc cttcactaaa
     6001 aacggctcgc tactgccgac ttcgtcaatc cctttacgct ttaaatcccg ctcacttaac
     6061 gcaatcacgg tacaacggca attaaagccg ttcggcggat aaaaagtcgc ccaaaacgga
     6121 tcgtcataac gataaatctt accctgtaat gcttggtgag cagggcgggt gcgttggtcg
     6181 cctaccgccg aatattgcca ataagggcga ttatccacat tatccatata ccgctgataa
     6241 cgagccgcag aataagcctg ttgcatattg gtacgataaa tcgtattcaa gcggcgaggc
     6301 gtgccgaaat gctcgccggt tttcgggtcg gcaagcagtt tgccatcaat gccacgacta
     6361 atcgtcgtgt ccatacaccc agcctttccg ttcaaattct gccgtaagcg ttttcttcca
     6421 ttcattaaag gatttcccct cacgccgtgc tttttccatc gactcataaa tatcacgggt
     6481 catttcaagg ctagtgagct tgctaatggt ggcggctcgc cccaatgcag aatcaaataa
     6541 ttcctgctta aacactttat tcgcaagcaa cttcttctgc cgtaaaaact caatcgcctg
     6601 cttaggctct agtccaatgg caaaattaac tttctgcatt cgcagcccct aataaatccg
     6661 ccaaaaataa cgcattcgct aaatagcggt cgtgttcttc actcactaaa tcagggtagg
     6721 ctgccgccaa tttttctgct gcctcatcat aagagctaca cgccattacc accgccaccg
     6781 cctttttcgt gatggggtca aattgggcgt taaaatccgg cacggaaaaa gcctcgtctg
     6841 ccaaatcatc taaggcatct tgctctttaa tgcccgtttt ggtttgggcc gacaaagcgt
     6901 gaaccttgcc cccacaaccg cattcgcaag cggtcatttt ttgcggaaaa tttgcggata
     6961 accccaccgc ttgtggcggg gcagaacgcc ctaacaccgc ttcattttct tgagcttcgg
     7021 gaataccgag tttatccatc gcccactgca ccggaatcgg cattccaata tccaccaatt
     7081 tcggcaagct ctcggcaaat aacgccaagt cggcaggctc tttggtatca aactcaaaac
     7141 tcggaatgcg ggttgggtcg atattgccaa aattaatttg cagaaacggc agaataattt
     7201 gttgggtaaa cgtctgcccc agctgtttca cgtccgacac caacaaatca cgccgcactt
     7261 cgttatgcac attgccgagt gcattggtag agcttttgcc gtctgcaccg ctggttaagg
     7321 tttgccccaa aatcaagcgg gcgatagact tctcacacca atccaccatt tgcaaaaacg
     7381 gattattgcc ggctgcaccg cctgcattag cggcgttatg caactcaata ctcatcgact
     7441 ccggcataat tccggcagcg ttgtgtccga tttccgctaa tgcacgttta agcgtttgtt
     7501 tttcgtttgg tgttgcccct gcaccgtatt taccaatgcg gataggcata ccatacagct
     7561 ccaaaaactc ggcaaagtcg tggatagagt agtgtttaaa catatacagc caagccagcg
     7621 agcggaataa attattgcgt gccgcctgtg tggagcggga tttatgcgta tgcaccaccc
     7681 agccaaattc tcgtaatggc tcaccttcgg tattttgtcg ggttttcagc aacagattgt
     7741 cgtgcttatc ccacttaaac cacgattgag gacggtggat aaaggcgttc ggataccaca
     7801 atttaccgtt aaacgcccat tcaatttcga gggcagcaaa accgtgtccc acagcgtcca
     7861 tacagtccat caccaaatct tcaaggttgc cgagctgata aaataactca tcaacctcat
     7921 cacgcagttt ttcttcctgc ggtgtggcat tgcgaggggc aacaatcgcc caatctacgc
     7981 ctaatgcggc acgcttacgg gtttggatat tggcaaaaat agagctgtcc cgctcctcaa
     8041 tatccataaa taattcgtgt tgagcggtaa tatcgccatt ctccgtatct tcaaaaatcg
     8101 ctttcatttt agcaggcgta ataaaattgc tcggatggtc ggagagtacc cgacctgtag
     8161 cggtaatttc ggctaaatcc gtctgcaatt cttgattttt aatcgggttt aaattcggtt
     8221 taatctgctt gttatgcttc tttttgctca taaattttcc ttttcagagc ttagttacgc
     8281 tcaatatagt ccccctcttt agcaaagagg ggagttacat taatgccgcc acttactgcg
     8341 atactcaccg tcaaaatcat ctacactctc ccactcaatc ggggcagagc tgctgagtgc
     8401 atttcgccat aacatttcca acgcatccgg accatcatcg tgatcggctt tcggaaaatg
     8461 gcgaagctgg ctttcaagcg tagattgcga gcggtgcaac aaaatcaaac cgttggcaat
     8521 atgcggttgc aagctctcaa tccgtaacat cttgtcactg ttcggcttgg ttgccgttgc
     8581 cggcaccggc ttaccacgct gggcagaacg cttgaccagt tccgtctgca aaaactcctg
     8641 aaattgcacc gtctccacaa accagcggtg acagttatat tgcgaatgca gtctaatcac
     8701 gtcctcaata attaaatccg gcaatcgttt tttgatttgt gcctcaacaa catagagctt
     8761 gccactctca cgatgatagc caccgaccaa aattgcagat gggtcacggc ttgccccagc
     8821 tttccccatt gaggggtcta acgccccgaa ataaatcagg tcatcaggca gttccgtcca
     8881 atactggata ctgttggcaa aaatcgcatc atcacccgag acagggtcat tttgatactc
     8941 ggagtcaaat gctgagtgcc catcactagc acggattttc atcaagtaga ggatagggcg
     9001 agctaaccaa gagactaacg ctcctttatc catttctgct ttatgctgtt gataaaagag
     9061 atcagacata gtgtcatcgt ccccttcttc cgagaggtat atattttccc attcgtccca
     9121 taacgccata ttgccgggaa agcgtaaaat ggctttaaaa cgaactcgcc gccaaccttt
     9181 tgtatttaaa atacggctaa gcacactgtc ataatgtaga attgtgccga catagataac
     9241 atcaaacttt tcacctgcag caccaagttt taaaactgct tttaaaaccc agttatgcaa
     9301 tttattacgc tgtgcaggtg tttctacgct ttcatcattc tcaatatcat caagtactac
     9361 taaatccgga cgataagctc cgtggcgacg accacgcaat ttttgcccag cccccacagc
     9421 ttcaactttt tgttttttag ccgtcataat gacacctgca cgccaaacct tgccttgtgc
     9481 aacctcgggg aaatcaattc ttaaacgagg gttagattca atttccactt taatagcttc
     9541 aagcatactg tatgcctgtt ctttggtgtc catcgcaata atgatgtatc tttttaaatc
     9601 acggactaaa caccacagcg ggaataattg cgtacaaatc gttgattttg cctcgccacg
     9661 aggggctgca atcgcttggc ggactgactt agaaagatca cttaccgaaa gcggtaaatt
     9721 ctcaaagaga taatgatgca actgtgattt atgttcagac cgcacatagt gtggaaaata
     9781 agcctgcaca aaatactcaa agccgtgaac agggtctaaa accttttgac ggcgttcgct
     9841 aatggcttgt ggtttatcgt cccaaccctc aaaactcgct tcgatatttt gttgtaactg
     9901 tcgccgtaat tcttccagtt ctttttcaaa ttctttgatt ttcataaatt acatacaagc
     9961 tgcgataagc aacaaccagc cccaaccgtc tgtaccgttg agcattagtt ttattgctcc
    10021 gaaaatacaa gcaagttgga caatccaacg gaaagagtaa tgcttatgca ctacagtttg
    10081 ttttttctta gccattactt aaactccttt ttgaattgca cttctagctc atctaacatt
    10141 tctaaaaagt caggcaataa atgcggtttc ttggttttga cgatattcgc catcatctca
    10201 atcgcacgca tagcggttgc cattgcactt gtttcaggca atagtttctt gctcgatgcc
    10261 gtcatcttcg caaacgaatc agccagagca gctaatgcct caaccttagc tgcggttggc
    10321 atttcggtgt tttcctcaag ctccgtcatc aacgtgcggt acttaattaa aaaaccggct
    10381 aacaagcctt gagcgatatt ctcaatacca ccgcttgcca tcgcttgcac atcacgagct
    10441 ttttcccagt tatcaccgtt cttttcagca gccgctttcc aacgccgtgc cgtgccgaat
    10501 gatacccctg cacgttctgc tgccatttcg aggcttaaac ggtcaaacac ataggcttgc
    10561 cgaaccgctt tttgtacttc aacatcgtgt gccatacgcc ctctacattc caaatttcag
    10621 gcgtaacaac tcaaaaccca cagcaacaat gccaccaccc aagccaccgg caactacagc
    10681 atcacggcgg tttttacgtg ccatttcatt cactaaacga ttagtcgctt ggatttcacg
    10741 gtgtaagcgt tcgatttctt cgttttgttt atccacctta tcgtttagct catttaatcc
    10801 acctaaaatc aggtctaatt tttccgaatc ggtttgcttt tgttttttac ggctcattac
    10861 ttatccgcct ttttatcaag tttttggttt acttctttga gcgagtctaa aatatcgtcc
    10921 agcttttcct taatccctct attcacttcg tgggcaagct ctttggattg atatttgctc
    10981 tccatttctg cttttagctc ttttaccgtt tgctcattgc ggttgatacg atcaaatatg
    11041 accttgccta caaacgccac taacggagca actacaaaag agatcagcat ctgaaacagt
    11101 ttttcatcaa tcataacgcc cccttttggc tcgcccgacc gggtgttgat taccttgtgc
    11161 caccgccgca cgcaccacgt tatgcaattc tgcaaggtca ggacggcggc gataaaaacg
    11221 ccattgttgc caacgctgtc tgagtttttt aattaacttc attctggcaa acttcctcat
    11281 aggtcagatt gtgtgcaagc acttggcgtt tcgtttccat tgtgtccgca cggctcgggt
    11341 aaatcagacc gaacgccgtg caacccgttg tcgtcacgga agtaaccgtg ttggtgcaac
    11401 tgctcatcaa tggcactagg ctgaatacgg cgagtttcac taacgtgttt ttgctgaatt
    11461 ttggcatttt taacctctgc atttttttgt tgaatttcga ccgcttgttg ctgcttttcc
    11521 gcctttaatg tagcggtaag tttccgctcc tgctttaatt gccagctttt ataaaaggca
    11581 taggctaaaa ttgtgcttac tacaccaacc acacctgcaa tttgttgagt aatcattctt
    11641 cagcctccct tgagcgttct ttttgtttta acgcacttac cacccctttt gtggctgggc
    11701 tacccaatac acaaatacca gcataagcaa accaccagtc ggaaataaac ggcttatcgt
    11761 aaaggtaggc gtgaattaaa atgcccgata acactaacca gctcataaac tgaataaagc
    11821 cagtggtgct ggctgtgccg tctttattgg taattaaatc aagtaaaaat tgcttcacga
    11881 ttattcctcc accgtgtctt tggcaaaata acgcaaatta cccgccattc ggttaatcca
    11941 acctctacca aaacggtcaa agtgtttgag tttggtgtaa aactcaaggc gttctgcctg
    12001 aaataacaag cccacatccg caaccgaata acgctcaatc gcagccaaag tcactttacc
    12061 gataatgccg tcatcagcca cacctaccgc tcgctgtaac attcggctag cattgcccga
    12121 accgtggttt acacacgcat ctaaaaactg aaaagccaac tcaggcggga actgctcaca
    12181 gttataacgt tgccaaaagg ctttgaggta aatagttttc gcatccgcac ggctcattgc
    12241 acgcatagag ccggtatagc cattcgcacg ggcagtatgg atagtgatac cccaattcgt
    12301 ttcgccaccg gggtctttcg ggtcgttcac ataaccgccc tcgtgtccga taagacggtc
    12361 aaatgcaaga ttaaagtcag caatagttgc cttgtttttg ataggttcag tcataaaaaa
    12421 agcctcttta aattcaatga tgatttaaag agactttaat gatttagagc aacggggagc
    12481 aggcgaaagg attccacact taaaacaagg tagcttgcga aacggtatgc ttacgctgat
    12541 aatatcgtac tgcctcccac gcaacacgat cacacacgct gtatttaggg caaatttgca
    12601 taatcgcaag cctgccactt agccctcctt gctcggttaa ttgacaataa tcggcataaa
    12661 tctgctgatt acgcaacatt cttaatgcgg tttcacaacg tggcaaatag acctcgcaag
    12721 ctcccatata agcctgtaat ttcactgcat catcttgccc taatacctct ttgagcttat
    12781 taaaatagag ttttccgtta gaaaattgga atgaaaagcc accaaaggct ttgattagct
    12841 tttcagttgc cggcaaacca atcacgccca caatttcttt caccgtttcc ggcaaataat
    12901 gttcgacact ttcaaattca gacataaaaa atccccttga ataatttcca cgaaagccat
    12961 tatccaaggg ggttattcaa atctgtaata cttacttaaa aatttttttg caaatttttt
    13021 aaaagctcag cccattttgt gtacggcata tctgatctag ctgcggttga tgacagttct
    13081 gtctcccacc gcattactat tctttgcccg acccctaata tgttagctac ctctgtttgt
    13141 gtgaggttat ggtgctttct tagcattttt aagttgcttg gtgtgtatcc aaactctggg
    13201 ttttcaatca ttcacctccc tccattaaac tagctattaa atacagcaac aacgataggg
    13261 tttctaataa tgctctcgcc tttatcttct ccgcctcttg cactatcgcc tttaactagg
    13321 ataaagtttt gcccacagta tgactggaaa cctttaaatt caacatcaaa ttttttgcaa
    13381 gcatcacgac ctagattttt aatagcattt aacaaacctg cttcatctgc attttggatt
    13441 cccattgtac aaatgccgtc taattcttca tctgtttttt caccatcgac ccaaacaaaa
    13501 gaatttccta attcatcgcc aacttgatgc acatctgtag ttgctcttaa accataaaca
    13561 gcgaagcagt cttcatcggc atcgtttagt aaagcattta aaagtttttg gtaatctgtc
    13621 attttaattt ctcctgtttg gctatgggtg cattatagta atataattac ctattgcaag
    13681 tgatttttcg tttttttctt aaattttttt atgtaaataa aaaagaccgc ttgaaagcgg
    13741 tctaaattta ttttttcttg gttgcagaaa tggattttgc aatggtaaag gcagtttccc
    13801 aagcctgttg attatctgta acctcaattt tacggcaagc aatcgcctcg ttgttaaagt
    13861 cagtcatatc aaccttgtcc gaattaacaa gcaaaataac acggcgtaaa gcacaagcag
    13921 caataggatt gtgatcgtgc ccaaacgaac cagtttccat tccgtaagct acatttctta
    13981 actgttgata atcaccactt acaagagcat ttgctgtttc ttgttcagct ttttctgtca
    14041 ttgctattgc tggtaacgac aataagcata aggttgttaa tagcagttct ttcattttaa
    14101 atacctttaa aagttgatta aaaaacacct aaaatctacc acaaaaaagc ccactattta
    14161 aagtgggcta tgcaataaat ttattattta acactcattc cgtgcctatg gttataactc
    14221 gccaatatct gcactaattt atgcaactga tcatctgtca gccattgcac ccgctcaata
    14281 ccaaacgagc gtttggcaat ggcgtgggcg taatcccacg gcttttggct ttgatacagc
    14341 aacgccccga ttttatttat caacccttgc cggtgagggg cggttttact tgctgttggg
    14401 cgtttaccgt attttttcga cttcaccaca aaccctttag ctttcattgt ttgcagtacg
    14461 gtcattaatt cgctatcggt catcattgag cagcttggtt tatccaccgc ctctaacaac
    14521 agcattttat aggcgttatc atccagtttt agctcatttt tgccgatatg gattttctgt
    14581 atcatctgct ttctagtttg cggttgcatt ttgcttttcc tcctgccatt tcagccaaat
    14641 ttgataactt tcggtgttct taacggcttc cagttgcccc atttgcttca ttcgttccac
    14701 atacatcacg gcatcgtgtt tcttttggtc tgcttgccgt tgctgttgct caaggctact
    14761 cactgctttc gcttcacttc ttactactgc aaattgtggc ttcacgcttt catatacttt
    14821 cttcaagtaa ttatgatttg ccagcggctc acatctgcca gtttcacgcc gattccgtct
    14881 tacttgttcc accgtatcca ttaaggcttt ttctaacaca ttagagggag ggtaaagctc
    14941 caataaactc tgcacaattt tcaaggctcg gctattggca aggctgtttt tctgtggctt
    15001 aaataaccct aaatacgcca ccaccgcacg cccacaattc ccaccgatat tgactatcgt
    15061 atttaacaac tcacgccctg catcatcttc aatcaacgcc tcaaacgaga tgtcggaatg
    15121 gcaaatcggg catttgcata gtttcatcat tctgttcctt cttctattgt ttcgagatca
    15181 acccaataag ttcgactatc agggtatggg ttatgccaag ttgctggagt tagctcgcaa
    15241 ataactaatt gttgtaacac aatctctccc gatttctttt tacataaccg atattcagga
    15301 attttttgat attccatttt atgctcctat aaaacacatt attcagccca cttaaatagg
    15361 ggttaaatgg gctgtaaatg ggttttatta cttcccctgt attgctaaaa actcactttc
    15421 tttaatctca accaatccct ctgcaatctc aggaaatggt tcatcatttt tatcaatagg
    15481 cattggcatt tcaaccagta aggtgttttc acaaatgcct gctacggcta aataacttct
    15541 acctgatgtt ggggaataac taaaatcact tacaaaataa agcatattca aacgttttaa
    15601 gatatagggc gaaaaatcac gatgtttctc ataaatttca ttaattgctt taaaatcttc
    15661 tgcaagttct ttcccttttt cgtaacgttt atcaggtttg acgaggtaaa actcatcatt
    15721 aaatttaccg accttgaacc ctttaacatc taaaacttgg ctaatattgg ttgctttaca
    15781 agcaatccct ataactattc tatgaaacca gcctgaccta ctaattaatc catcattgaa
    15841 aggatatttt tctaaaatag cattgagctt atccttgcgt tcttctttag atacttgata
    15901 atctgcataa atgcttttta gtggctcttg ggttatatcc cctttaaaaa atctaaattt
    15961 cattcttaac tccttactgg ggtggttaat gtttccgatt tgccgttaat aacttcaaat
    16021 gcacctttgg caacaaatcc tgcaccgtgc cgagataaac accactgcga ataaagtcac
    16081 tctgttttaa atatttttgt gcctgaataa tctgcattaa cgcctcgttt aattggctgt
    16141 taagttcgtt tttctcttgt tcggtcatca tctcctccta aaacggtttc ttatgcatac
    16201 gtttacaaaa taccgctcgt tgccccgccc agtctcagtt agcggtcttt tttgctgata
    16261 attttgcaac tttccaatgg tcttgggctt ctgcataatt gcctttacgt tcggcttccg
    16321 ctgctttctc ggcataatgt ttgtagcggt caaactcggt cgccttttgt tctgttttca
    16381 ttttttcctc ctgtggttaa aacactttat aaatgccctt ctatcttgtc cccctctttg
    16441 aaaaagaggg gctaggggag atttttaaag ggcattgaaa aggtttttta actaagactt
    16501 tctttttaac tgatttaaaa tatgcaaatg atttaataac gatgcatatt ctttatcatc
    16561 aaagtccatc attattaatt ttttgttatc acaaaagtgt actgttacat tcccgttaat
    16621 attttctatt gaacaaacct tgtctgtatt aaccacaaca ggtttatccg tataattaac
    16681 attaaaagta ataaacattc taaacccccg ccatatctaa actaatcggc tgatactttc
    16741 cgttttcatc acgtttataa aaacggatat aacctttact accgatcact tgtacgctat
    16801 ctgaaatcgc tgtcattgct tgattccatc ttgcatcatc aatctcaaca cggcggagag
    16861 agagaatttt tgctgtagat aaattacctt ccttatccac ctcaaacgca ttatcaatta
    16921 aggccttaag ctccggtttt gccccttccg accaatcgtg caagcattca tcaatcaagg
    16981 cttttgctgc gtgaatacgc tcatcaaagc gaatattctc ttgcactgct aactgcaact
    17041 tatattcacc gtcataagta aatagtgtga tattgccttt tgcaccgcca atttttactc
    17101 catatttgtc agccgatagg ctgacaaaag cccccacgtc atcaaatacg gattgtttaa
    17161 aatcacctaa cgctttgctt aacgcttgtg ctttgcttac aaattggcga actaattcat
    17221 cacgttcttt gtcgatttct tttactaatg cctccggctt taaattgcca tcagcatctt
    17281 tccagtaaat ttcattaccg attgttactt tactcataat acttcctctc tattccaacc
    17341 cgactttcac ggtgcggatt gctttaccgt tatatttcac tcttttgttg tgagcagggg
    17401 acgacataaa ccgcactgtt ttcgcctcca cccccaaccg ttctgctaac tcttccgccg
    17461 taccgtcagc cacattctct tcgccaacat acagggcata aatcattcgc cgttttttca
    17521 tttctacacc ttcgtaacac gttcagactc ccacacgcat ttcacccccc gcacctgcat
    17581 ttgatagctg tcataccgtc tgccgccttt ggttttcatt ccaaacaggc ttgccaagcc
    17641 gttcttgact aaatcctgtg ttttgcagtt atcccgtaag accaagcggg gtgggctttt
    17701 cgcttcaaac tccactcgtt caacttccaa ctccatcgcc tcacattcca aagtggcaat
    17761 ttctaacttc acaaggctgt cgtggataaa cgcatttaag gggttcatca tttcgcctgc
    17821 tttttcgctg taaacctttt gcataacttg ctccttagct aatcaacatc ttgctatatt
    17881 gctcaaccaa atctttacta attggtgttt ggttaatctc gctagaccgc accacgccac
    17941 gcattaactt acttaatcgg cgggcattgc cgtgacaagc tttaattagc ggggcattaa
    18001 attcatttgt acctaacgca ctttcggcta atagccccaa atcttcctcg ctcaaggcat
    18061 ttcccaaatc acaagcaaaa cctactcggc tataaagctg tgccagctca ttatttttac
    18121 ctttaagatt aaccaacaat ctcggcatac cggctaacac cacgcccacg ctggttaaat
    18181 cgtggattcg gcggataaac tccagcgagc gggtcgaaag taattcagct tcatcaatca
    18241 ttagcaacct ttcagagcct tttaacttgt tcacaatgct ttctaatagt tcgttattta
    18301 cccctctggc atttgccccc accgcctcgg caatcttgcg gagcaagact ttaggtgtgc
    18361 aactcggatc aacctcaatt aatgttgcag agctgtttct gcgttcgtat tctttgagca
    18421 tttgggtttt acctaagccg gctgccccaa aaatcacatt aatttcgcct tcaatatggg
    18481 caaactgcat tacctccatt cctctgcgtg ccgccaaagt tggcacaaaa gcagcgttat
    18541 atttcgcttc caccactttt gccttatcac gctcaatcag ctcgtctact ttgcgatcca
    18601 tttcagccac attgccgtta taatcgcctt tgagatacag actgactgtg gcaacggata
    18661 cacctaaaag ctgtgccact tgtttttgtt gatagccttt gctatccatt aaggctctaa
    18721 gttcttggtt tttcatcgtt tagcctccca ctgctaactt tttttcgtaa tcctcttttt
    18781 ctgctcgggt caaaaaaatc ggttctactt cttttttcgg ttttcttgcc gttgccagca
    18841 agctaaaatc gtttttctgc tcaatggtta aaaccgggtt taattccgca ttaatctcat
    18901 caagctgttg ctgtttaagg ttggctcggc gtttatggcg ttctttacgc tgctgttcta
    18961 ccatcgccac cgggaatgcc tctcgggtgt taccgttcca aattgcctcg caaataaacc
    19021 tgccgtcttt ggtacgcacc tgcacgctct cggcattgtg aatatcaatc ccgatcacca
    19081 cttcttcacg gtcaaaatcc agtaatttca agttaaaata gtggttgtta ttccaactta
    19141 cccaacctcg atccggctta cggataaatt ccggtcggct catatcccgc aactcaatag
    19201 ggcttaacat caccacatct tctgcgtgca acatctgctg atatttgcgt gccggcgtgg
    19261 tacggatttc cgagtgaata tgctcgttgt tgtaccagtc aatcaccgat tggattactt
    19321 caatcaactg ttgccacgtt ggcaatttgc cttgggcttt acgttgtagt ggggttaatt
    19381 cccctttggc atttgccagt gagttcaccg catacagggt tttgcgaacg gtttccggat
    19441 ctgcaccggt tgcatagccg gtttcaaact gtcgggcaat cgtcataccg atggttttat
    19501 ttaagcgttc aataatgcct cgcccctgcg gattgcccgc aatccctgtt tcgtgtcgga
    19561 taccgaggcg gggcaacatc cccgtgattt ccgcatccaa aaatttattc ttttcaccac
    19621 caccgttatc cgagtagtag attgccggca caccgtgtag gctaatggcg tggcgtagtg
    19681 catcagccac cgcaaaggcg ttctcactga gtgccagcga ccagcccacc acttttctac
    19741 cagcaccatc gacaatcatt gtcagctcag gggtaaacgg cttaccgtgt atcgggtggg
    19801 ctactttgag cttcagtgag tgaccgtcac caatccatac atcatttgct cgaagtaccg
    19861 accaatcccg cttcacatag ctcagtaact gcttgtaatg cgagccggtg cgtctgccgt
    19921 actctttcac atacaacggc aacttattca tcgctcgctg tacttggctt aggctggggc
    19981 attgtgccaa cagtagcggg ttgtctgcat actgatacgc ccattccacc tcaaattctc
    20041 ggtaggcttc ggtgaggcaa ataccgtttt tctgccgata tacgcctaaa aaagcactta
    20101 gccaccaaat ttcttccggc ttggtctcct gccgtacctg tggggcaagc agtttcaacc
    20161 gttgctcggc gttctctgcc ttgcagtaat ccaccaccca gccgtttaag gttcgcaccg
    20221 agagtgtgcg ttttgccgtc tttttagcat ttgctacggc aaccagctcc gccaagtgcg
    20281 gtggcatttc gccttgtttg gcaaggttgc aaaggtaggt tatcgcttta atccggctca
    20341 ttgagccttc caactccaac acatacgcca ccagtgccat tcgtgcatca gcaatttctc
    20401 gctgtttggt ggtcaggttt cccacatcca cctctgcacg cactaccggt aactttttcg
    20461 gcttggcttc caccaccgcc gacataaagc gggagcggat ttcggtttga acttcggtgg
    20521 gtaaagagtt aatgtgatat tcaaaaccac ctcctttaac ccctttcaat ggacgagttg
    20581 cccattgttc ttttttggct tgtctagtta catttgttgg gtattttgat aagccactaa
    20641 tacctgctaa ttcttttgca ctaaaccaat ctttcataac agctatcctt gttatgcaac
    20701 atcagggtaa cgactaggcc atatttcggc tggcgttttt cctaatgctt ttgcgataat
    20761 ttcttcaccg tttttataag gtttatcaag agcatttctt actgttgttt tggaaagccc
    20821 attctcaatc cctaactgag aaaatgttat tcctttcttc atcaattcag ctcgaatatc
    20881 agctctatgc atatcttgag ctcttttctt tttattcatt ttgtgagatc ctccacattt
    20941 agtttatttg ctacgcttta gcgtacttga
//
LOCUS       DANLXS010000021        20072 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.021, whole genome shotgun sequence.
ACCESSION   DANLXS010000021 DANLXS010000000
VERSION     DANLXS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20072
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.021"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..685)
                     /locus_tag="PXD71_000632"
     CDS             complement(<1..685)
                     /locus_tag="PXD71_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL6290787.1"
                     /translation="MILFAGDPHGSYEHLYPILQQQTEEVALVILGDLQLTTPDELDR
                     LSQYCDIWFIHGNHDSKTVAAFDAIWNSSWKERNIHGKVVDIQGIKIAGIGGVFRGHI
                     WMPPNRPMFFDPIHFCQYSAPEKIWRGGIPLRHRSSIFPSDFEALENEKADILITHEA
                     PKPHPQGFSVINQLARKLGASKIFHGHYHDNFNYGNLNVSRECAIYNIGFRSLADING
                     NYLILGVDDR"
     gene            complement(703..1977)
                     /gene="nadR"
                     /locus_tag="PXD71_000633"
     CDS             complement(703..1977)
                     /gene="nadR"
                     /locus_tag="PXD71_000633"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDL6290788.1"
                     /translation="MTDFAYLQQKRKQLKLKVNDICEQAGVTRAYFNQLVSGKIKNPS
                     ANKLKALHSVLDIVEDSNQRSGVIFGKFYPIHTGHINMIYEAFSKVDVLHVIVCTDSE
                     RDLRLFQESKMKRMPTNEDRLRWVQQIFKYQQKQIFIHHLVEDGIPSYPNGWEDWSGR
                     VKALFEEKSINPTIVFSSEIQDKESYEKYLNLEVQLVDPTRESFNISATQIRNNPFQY
                     WRFIPKEVRPFFVKTIAILGGESSGKSVLVSKLANVFNTTSAWEYGREFVFEQLGGNE
                     QAMQYSDYPQMVLGHQRYVDYAMKHAHKVAIIDTDYITTQAFCIQYEGKAHPFLDSMI
                     REYPFDVTILLSNNTKWVADGLRSLGSKKQRQQFQHLLKKLLEKHHIPYIKIESPSYL
                     DRYNQAKEVVEAILNEEPIPLQFKQYNIHEFE"
     gene            2201..3163
                     /gene="ribF"
                     /locus_tag="PXD71_000634"
     CDS             2201..3163
                     /gene="ribF"
                     /locus_tag="PXD71_000634"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDL6290789.1"
                     /translation="MQLIRGFHNLDKHIALNQGCVLSIGNFDGVHLGHQNILARLCDR
                     ALDLGLPSVVMLFEPQPREFFAKKSGNSTACNPPARLMRLRDKLKYLEEAGVDFVLCV
                     RFSEKFAQISADDFISDLLAKRLKVRYLSVGDDFRFGAKRAGSFETLRNAGLKYRFAV
                     EESHTHSLNEERISSSLVREALQNNELDLAEKLLGKPYSILGRVAHGNKLGRTIGFPT
                     ANIMLNRLVTPIQGVFAVKVKTKQGSFNGIANVGNRPTINGTKPLLEVHIFDFNASIY
                     GEAIEVIFLKKLRNEIKFPDFEALKAQIEKDRLQAVEFFKEFAR"
     gene            3266..6082
                     /gene="ileS"
                     /locus_tag="PXD71_000635"
     CDS             3266..6082
                     /gene="ileS"
                     /locus_tag="PXD71_000635"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL6290790.1"
                     /translation="MTIDYKNTLNLPETGFPMRGDLAKREPDMLKNWYDKNLYQKIRT
                     ASKGKKTFILHDGPPYANGNIHLGHAVNKILKDMILKSKTALGFDTPYVPGWDCHGLP
                     IELKVEGIVGKPNEKISAAEFRQACRDYAKEQVEGQKADFIRMGILGDWDNPYLTMNF
                     NTEANIIRTLGKVIANGHLYKGSKPVHWCLDCGSSLAEAEVEYEDKVSPSIYVRFNAV
                     DVNAVEEKFNAQGKGRGALSAVIWTTTPWTIPSNRAIAINPELDYALVQLGDERVLLA
                     VDLVEDVAKAAGVESAEILATTKGENLELLRFNHPFYDYSVPFIFGDHVTTDGGTGLV
                     HTAPDHGADDFVVARKYNIEMAGLISNDGKFKADTPFFAGLGVFESNDKVVEKLQEVG
                     ALLKLSRIKHSYPHCWRHKTPIIFRATPQWFIGMETQGLRQQALGEIKSVRWIPSWGE
                     ARIDTMVANRPDWCISRQRTWGVPMAMFVHNETEELHPRTLELIEEVAKRVEEKGIQA
                     WWDLDPAELLGEEAKDYRKVPDTLDVWFDSGSTYASVVEQRPEFNGNSADMYLEGSDQ
                     HRGWFMSSLMLSTATNGKAPYKQVLTHGFTVDEKGRKMSKSLGNVIVPSEVWNKNGAD
                     ILRLWVASTDYTGEITVSHNILNSAGESYRRIRNTARFLLANLNGFDPKSDLVQPQEM
                     IALDRWAVACALEAQNDIKEAYDNYQFHTVVQRLMRFCSIEMGSFYLDIIKDRQYTTK
                     ADSLARRSCQTALWHIAEALVRWIAPILSFTADEIWGYLPQVEGRSEFVFTEEFYTGL
                     FGLTEQDKLDDAYWQQILKVRAEVNRVLEQARKDKVIGAGLEAKVTVFANDEIRPLLE
                     QLGNELRFVLITSQAIVKPLAEADIAEGELAGLAVKVENADGEKCPRCWHYATDIGSH
                     SGHEEVCGRCVENVAGEGEKRLFA"
     gene            6207..7754
                     /locus_tag="PXD71_000636"
     CDS             6207..7754
                     /locus_tag="PXD71_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HDL6290791.1"
                     /translation="MMKIIAFLFIFLSVFSHTALANSMAEQCVALKSNSPEKTAIYKA
                     EWVEKSAELPAYCLVQGEIGKRVEVDNVPYGIQFELRLPEQWNEKFLFQGAGGVGGVI
                     FPAIGKLYPHGASGANGLNRGYAVVSNDSGHATRDLVFTADPEARSNYAYASIGKVTT
                     VAKQLIAEFYRKPSKHNYIMGCSNGGREAMMAAMRYPQEFDGVIAGSPGFRVSRSVLA
                     EVWDNRALLAVAPKNGDGDKILSQALTQQDLDVIANGVLTRCDKLDGLADGLINAWEQ
                     CDFQPEMVAKQLGQKKVALIKTIFEGAKNSRGELIYSPWAYDSGINAKGWRDWKLGDS
                     QTAQPNSISFKMGLKSLTHYYLAPRVPSRDPLSVDLDQASEQVKAVGSIHDTDQVDLS
                     AFKQNGGKMLIYQGVSDPIFSAVDLKNWYQSLQQAVENPQHFAKLFFVSAMNHCGRGA
                     TVNDFDMLTALENWVEKGLEPESIAAKAGELYPNKAKQIPLCAYPKVAIYQTGEDPNR
                     LTSYQCR"
     gene            7824..9191
                     /gene="pssA"
                     /locus_tag="PXD71_000637"
     CDS             7824..9191
                     /gene="pssA"
                     /locus_tag="PXD71_000637"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDL6290792.1"
                     /translation="MLILNKQRKAEYLLKSLPYLPQVAEQVQFLASSKAFKQQILQLI
                     QTAKKRIYITALYFEKDEAGQEVLAALYQAKRNNPELEINILVDWHRAQRGRIGEAEN
                     LSNADWYAKVKYEQGLPAGQEIAFYGVSINGREVFGVLHLKGFVFDDTILYSGASINN
                     VYLHQFERYRYDRYHVIENKVLADSWVTFIQQHLLTNEAVKRLDTENRPKTAEIRAQI
                     KHFRKQLSREAYLFNGVAENGRLAVSPLVGLGRRKNQLNKVIEALFYQTEQKLTICTP
                     YFNFPHSLRTRIEWLLANGKSVEIIVGDKTANDFYTKPDEKFTMASALPYLYEKNLRA
                     FAKRLDGYVQNKQLTIRLWKDGENSYHLKGVWVDNRYILLTGNNLNPRAWRLDAENAI
                     LISDPQHQLSEKAETELNQICQHTQILTHYSDLEVLTDYPENVRKLLKKFGRVKLDKI
                     VKMLL"
     gene            9246..9989
                     /gene="rlmB"
                     /locus_tag="PXD71_000638"
     CDS             9246..9989
                     /gene="rlmB"
                     /locus_tag="PXD71_000638"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL6290793.1"
                     /translation="MSEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLMPLLNELQ
                     RLGVAIQQVNRQTLDNKSQGEVHQGIIARVVPQKELNEHDLEAILSQKKNPLLLILDG
                     VTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTPTARKVACGAAEVMPLIRVTNLART
                     MRDLQERHNVWIVGTAGEAESGIYEAKLTGSIALVMGAEGDGMRRLTREHCDQLISIP
                     MAGSVSSLNVSVATGVCLFEIVRQKLAGQ"
     gene            complement(10076..11488)
                     /locus_tag="PXD71_000639"
     CDS             complement(10076..11488)
                     /locus_tag="PXD71_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6290794.1"
                     /translation="MKLLDIQNLSVETENRLQLVEPISLQLKAGKNITILGETGSGKS
                     LLIQAIMGALPDGLIASGEISVKNCKISTESDRLQTLWGKTLVMLPQEPRRSLNPIMA
                     IGKQLWESFHYVAGKPSSQAKTESKAYLAKLGLGESVENYPHQLSGGMAQRASFAIAT
                     AAGGKILLADEPTKGLDPISKGKVIELLKSAYQNGGGLLTITHDIEVAEQLGGTILVM
                     KKGELLEQGEAKALLNNPQHPYTKALISADPKHWQPLQDLQNLSQKQPLVSVKNLSIA
                     RGKKTLFSNLSFDLHKGEVLGIVGHSGIGKSSLADVLCGLLKPKSGEVVWHSHSHKKH
                     QVLKLYQDPPEAFAPNVSLKTLLDDVINKHQLDRLPIPSLLQQLSLNPEILQRNAENV
                     SGGELQRVAILRALLLEPVLLFADEVTSRLDPITQQETIELLINQCRARNCSLILVSH
                     DPYLIEKSCDKVINLEEYVG"
     gene            complement(11577..12359)
                     /locus_tag="PXD71_000640"
     CDS             complement(11577..12359)
                     /locus_tag="PXD71_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6290795.1"
                     /translation="MKISISQYLGGTILLAMLGFAFLQPYFYPMDIGFQDLTNILSNP
                     NEIAWFGTDHLGRDMLARLASAIRLSFGLSLFSVICALIAGLITGIMAGFFGGWIDRI
                     FSFICDLVMALPGLLLVLLFAALSPGSFWTLYLGISLVMWVEFFRVIRSISSTMANSS
                     EIQASRLMGMGWFYSFKRHLLPRLLPMIATLSAFSLGNAILALATLGFVSVGLRPPTA
                     ELGLMMTELFPYYYEAPLIFLQPVVAVFLMVLSLQLLSRKIK"
     gene            complement(12369..13301)
                     /locus_tag="PXD71_000641"
     CDS             complement(12369..13301)
                     /locus_tag="PXD71_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6290796.1"
                     /translation="MHKILFRRLIQILLVIWSVGTLTFILTRQLSGDMAYRIAASRYG
                     YDQADSAAAEAVRAELGLDQPWWQSYFSWLSDLAQFNLGKSLVTGDPVWSEIQHQFGH
                     TLSLAIVALILAILIGPPLGLLAARKPNGFFDRLTLVFSTLFRSVPAFIIAIGLITLF
                     SAQLKWLPAGGYGCWQHFILPALTLAIGLSTVSVRVTRSAMVQVKGSEFYQFSQLKGL
                     SRWHTFLRHGLPNIAVPVIAYHAVQLVYLIEGVVVVESLFAWPGSGHALVHAIIARDV
                     PMIQGTTLVMGGLFVVLNTIADLLSAWIDPRIKR"
     gene            complement(13521..15095)
                     /locus_tag="PXD71_000642"
     CDS             complement(13521..15095)
                     /locus_tag="PXD71_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6290797.1"
                     /translation="MKKWLIPLTVAAFCVSACDDKNSTQATAEKPQVENTQSQKNAEI
                     LPLATVVAPWEINSLDPNQSGVIFQRMNLAETLVEADLNGNLIPALATDWRSNEEGTI
                     WRFTLREGVKFHNDQVLTAQAVVNSLNIAKSKPGVLAKAFIKEIRAVDNKVEIELTKA
                     FVPFPPFLTHYTALILAPESYNEKGEVTQIIGTGAFKPTKIEAPQKLEAVQFEGYWGK
                     KPQVQQANYLASSRSETRMLMAQSEPSSLVFNLDTASVARLKADPNLQLVSGSIARTI
                     QLKMDVAKPFFNDLAIRQALSQAINRKAIAEQVLKIENGAADQILPKAFADWRVDTAE
                     QVVDIAKIKQNLTASGYTFDEQGRLTKDGKPFSFTMRTFSDRPELPVIATILQAQWKQ
                     IGVEVNVSVGNFSEIPAGHQDGSLEMALYALNYGKTLDPYGVIASDFANGGSDWGVMN
                     WQNTEVDNAVKSLETERDPQKAKALKQTVSKIIYNDLPIIPVVYYQQNVVAHKEVKNV
                     TLDPFERRFFLELLSK"
     gene            complement(15354..16934)
                     /gene="prfC"
                     /locus_tag="PXD71_000643"
     CDS             complement(15354..16934)
                     /gene="prfC"
                     /locus_tag="PXD71_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL6290798.1"
                     /translation="MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVK
                     GKGSAQHAKSDWMEMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTA
                     VDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
                     IKCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEVRVVKGLNNPELDQAVGD
                     DLAQQLRDELELVQGASHEFDHTEFIKGELTPVFFGTALGNFGVNHFLDGLTEWAPAP
                     QARQSDEREVSPSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIG
                     KDVVISDALTFMAGDRSQAEEAYAGDIIGLHNHGTIQIGDTFTQGENFKFTGIPNFAP
                     ELFRRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLINNDLIVGAVGVLQFDVVVSRL
                     KTEYGVEAIYETVNVATARWVEGKDAKKFEEFKRKNEANLALDGGDNLTYIAPTMVNL
                     NLAQERYPDVQFFKTREH"
     gene            17156..18031
                     /locus_tag="PXD71_000644"
     CDS             17156..18031
                     /locus_tag="PXD71_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL6290799.1"
                     /translation="MMKKFIPVMIAALVLSACSSQPAKRKSTPRIKATAAYIPTENSA
                     YVVFDLNQKKFLEGKNFNLVRPIASVTKLMTAVVFLEENRYGASCQTQILPSDADFLK
                     GTTSPLPKNLNIACSELLKAMLVRSDNYAAHALAHATHLNKAQFLARMNSKAREIGMT
                     QTYFGDSSGLSSDNVSTVVDLAKLAGYAASKPEIQQLSNSPLVSVYAGGRYFSMKNTN
                     SMVRSQSYQALVSKTGYTREAGYNLAFIAKGDCRGKRIGVVSLNNDNAASRTDFTESM
                     LAKYQCVPNPYRQYD"
     gene            18104..18691
                     /gene="pth"
                     /locus_tag="PXD71_000645"
     CDS             18104..18691
                     /gene="pth"
                     /locus_tag="PXD71_000645"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL6290800.1"
                     /translation="MSEIKLIVGLANPGVKYEETRHNAGEWLINELARIYNVSLKEEP
                     KYFGKVAKINTAQGEVRLLVPTTFMNLSGKAVGALANFYRIKPEEILVAHDELDLPPG
                     SVKLKKGGGHGGHNGLKDIIAALGNNKEFYRVRIGIGHPGHKDLVANYVLGKPSPSDQ
                     TLLNSAVDEAARCVEVLFKDGMVKATNRLNSFKAN"
     gene            18877..19968
                     /gene="ychF"
                     /locus_tag="PXD71_000646"
     CDS             18877..19968
                     /gene="ychF"
                     /locus_tag="PXD71_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL6290801.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNTGVV
                     PMPDPRLDALAEIVKPERVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGH
                     VVRCFENDDIVHVAGKIDPADDIEIINTELALADLDSCERAIQRLQKRAKGGDKDAKF
                     ELSIMEKILPVLENAGMIRSVDLDKDELHAIKGYNFLTLKPTMYIANVNEDGFENNPY
                     LDRVREIAEKEGAVVVPVCAAIESEIAELDDEEKVEFLQDLGIEEPGLNRVIRAGYRL
                     LNLQTYFTAGVKEVRAWTVSVGATAPKAAAVIHTDFEKGFIRAEVIAYQDFIDNKGEA
                     GAKEAGKWRLEGKDYIVQDGDVMHFRFNV"
BASE COUNT         5781 a         4075 c         4570 g         5646 t
ORIGIN      
        1 ctcgatcatc aacacctaaa attagataat ttccattaat atccgctagg ctacgaaagc
       61 cgatattata aatagcacat tctctgctga catttagatt gccataatta aaattatcat
      121 ggtagtggcc atggaaaatt ttgctcgccc ctaattttct tgctagctgg ttgattacag
      181 agaagccttg agggtgtggc tttggtgctt cgtgtgtaat taaaatatcg gctttttcat
      241 tttccaatgc ttcaaaatca gaagggaaga tcgaagagcg gtgccttaat ggaataccac
      301 cacgccaaat tttttccgga gcagagtatt ggcaaaaatg gatgggatca aaaaacattg
      361 gacgattggg cggcatccaa atatgacctc ggaagacccc tccaatacct gcaattttta
      421 ttccttgaat gtcaactact ttaccgtgaa tattgcgttc tttccagcta ctattccaaa
      481 tagcatcaaa ggcagcaacg gttttactgt catggttacc atgaataaac caaatatcac
      541 aatactgcga aagccgatct aattcatcag gagtagtcaa ttgaagatcg cctagaataa
      601 ctaaagctac ctcctccgtc tgctgttgta gaatcggata aagatgctcg tagctaccgt
      661 gtggatcgcc tgcaaaaagt atcatgttat ttcctgtggc agttattcaa attcgtgaat
      721 attatattgc ttaaactgta atgggattgg ttcttcattt aaaatagctt ctaccacctc
      781 tttggcttga ttgtagcgat ccaaataact tggagattca attttaatat aaggaatatg
      841 gtgtttttct agtagttttt tcagtaggtg ttgaaactgt tgacgctgtt ttttcgatcc
      901 taaactgcgt aaaccatcgg ctacccattt tgtattgtta gaaagtaaaa tcgtcacatc
      961 aaaaggatat tcgcggatca ttgaatccag aaacgggtga gccttccctt catactgaat
     1021 acagaacgct tgagttgtga tgtaatcggt atcaataata gccactttat gagcatgttt
     1081 catggcataa tcaacgtagc gttgatgtcc aagcaccatt tgcggatagt cggaatattg
     1141 cattgcttgt tcattgccac caagttgctc aaacacaaac tcacgcccat attcccaagc
     1201 agaggtagtg ttaaatacat tcgctaattt gctcaccaac actgatttac cactgctttc
     1261 accacctaaa atcgcaatag ttttaacaaa gaaaggacga acttctttcg gaataaagcg
     1321 ccagtattga aaaggattat tacgaatttg tgttgctgaa atattaaagg attcacgagt
     1381 aggatcgaca agttgaactt ctagattcaa atatttttca taagattctt tatcttgaat
     1441 ttcactacta aaaacaatag taggattaat gcttttttct tcaaatagcg ctttaacacg
     1501 accagaccaa tcttcccagc catttggata gctcggaata ccgtcttcaa ctaaatggtg
     1561 aataaagatc tgtttttgtt ggtatttaaa aatttgctgt acccagcgta gccgatcttc
     1621 atttgtcggc attcgtttca ttttactttc ctggaaaaga cgtaaatccc gttcagaatc
     1681 ggtacaaaca ataacatgta aaacatctac tttactaaat gcttcataga tcatattaat
     1741 atgacctgtg tgaataggat agaatttgcc aaaaataacc ccggaacgct ggttgctatc
     1801 ttctacgata tctaaaacgg aatgcaaggc tttaagctta ttggcgcttg gatttttgat
     1861 ttttccgctt actaactgat taaaataagc tcgggttacc ccagcttgtt cacaaatatc
     1921 attcactttg agtttaagct gtttgcgctt ttgttgaaga taagcaaaat ctgtcataat
     1981 gattccctcg attttttgat gattatcata tagtaaaatg tagcatttga atatattttt
     2041 attgtataaa tagcagtttt atagcatatc tttgctttaa taattagcta cacgaccact
     2101 ttcagcacta aaaaaatata acagattgaa tttttttcct tttttgtaga tttctcgtta
     2161 taatcacagc aattttcact tataaaatgg tttatctttt atgcaactta ttcgaggttt
     2221 ccacaactta gacaaacaca tcgctttaaa tcaaggctgt gttctctcta tcgggaattt
     2281 cgatggtgtg catcttgggc atcaaaatat tttggctcgt ctttgtgata gagctttaga
     2341 tttaggattg ccctcagttg tgatgttatt tgagccacaa cctcgagagt tttttgcaaa
     2401 aaaaagtgga aattcaaccg cttgtaaccc gcccgcccgc ttaatgcgcc tgcgagataa
     2461 gctaaaatat ttggaagaag ccggtgtgga ttttgtgcta tgcgtgcgtt ttagtgaaaa
     2521 atttgctcaa atttcggcag acgatttcat cagcgatttg ctcgctaaaa gactgaaagt
     2581 acgctattta agtgtaggcg atgattttcg ctttggggca aaacgggcgg gcagttttga
     2641 aactttacgc aatgccggct taaaatatcg ctttgcggtg gaagaaagcc atacccatag
     2701 cttaaacgaa gaaagaatta gcagctcatt agtgcgagaa gccttacaaa acaatgagtt
     2761 agatttggca gagaaattac tcggcaagcc ttattcgatt ctgggacgcg ttgcacacgg
     2821 taataaactc ggcagaacca ttggttttcc taccgcaaat attatgctca atcgtttggt
     2881 tacgccgatt caaggggtat ttgcggtaaa agtgaagacc aaacagggca gttttaacgg
     2941 aatcgccaat gtcggcaacc gcccgaccat taacggtact aagccgttat tagaagtgca
     3001 tatttttgat ttcaatgcct ctatttacgg tgaggcgatt gaagtgatct ttttaaaaaa
     3061 attacgcaat gaaataaaat ttcccgactt tgaagcacta aaagcccaga ttgagaaaga
     3121 tcgcttgcaa gcggttgaat tttttaagga atttgcaagg tgagcctttc tagtttccag
     3181 ccccagaggg gctgagctat gcttaaccta gtacggctat gccgtgctag gcataaaaca
     3241 gataatttta acgagaaata aaaaaatgac tattgactac aaaaacacct taaacttgcc
     3301 tgaaacaggc tttcctatgc gtggggatct cgcgaaacgt gaacccgata tgttaaaaaa
     3361 ttggtatgac aaaaatttat accaaaaaat tcgtactgcc tcgaaaggca agaaaacctt
     3421 tattttgcat gatggtcctc cgtatgctaa cggtaatatt cacttaggtc acgcagtgaa
     3481 taaaatttta aaagatatga ttttgaaatc gaaaaccgca ttaggtttcg atacccctta
     3541 tgtgccgggc tgggactgtc acggtttgcc gattgagctg aaagtagaag ggattgttgg
     3601 taaaccaaat gagaaaattt cggctgccga gtttcgccaa gcgtgccgtg actatgcgaa
     3661 agaacaagta gaaggtcaga aagccgattt tatccgtatg ggtattttag gtgattggga
     3721 taacccatac ctaacaatga attttaacac tgaggcgaat attattcgca cgctcggcaa
     3781 agtgattgcg aacgggcatt tatacaaagg ttcaaaaccg gtacattggt gcttagattg
     3841 tggatcttcg ttagcggaag ctgaagtgga atatgaagat aaagtttctc cctctattta
     3901 tgtgcgtttt aatgctgtgg acgtaaatgc ggtagaagaa aaattcaacg cacagggtaa
     3961 aggtagaggg gcattatctg cggttatttg gacaactacc ccttggacta ttccgtctaa
     4021 ccgtgcgatt gcgatcaacc ctgaattaga ctatgcctta gtccaattag gtgatgagcg
     4081 tgtgttacta gcggtcgatt tagtcgaaga tgttgcaaaa gcagcaggcg ttgaatctgc
     4141 tgagatttta gcaaccacca aaggcgaaaa cttagaatta ttacgcttta accacccatt
     4201 ctatgattac agcgtgccat ttatttttgg cgaccacgtt accacagacg gtggtacagg
     4261 gttagtgcat actgcaccgg atcacggtgc ggacgacttc gtggtcgctc gcaaatataa
     4321 cattgaaatg gcagggctaa tttccaatga cggtaaattc aaagcagaca ccccgttctt
     4381 tgccggctta ggtgtgtttg aatccaacga taaagtagtc gaaaaattac aagaagtggg
     4441 agcattatta aaattatccc gcatcaagca tagttatccg cactgttgga gacacaaaac
     4501 cccgattatc ttccgtgcta ccccacaatg gtttatcgga atggaaaccc aaggcttacg
     4561 ccagcaagca ctaggagaaa tcaaatcggt gcgttggatc ccaagctggg gtgaagctcg
     4621 tattgatacg atggtggcaa accgcccaga ctggtgtatt tcacgccaac gtacttgggg
     4681 cgtgccaatg gcgatgtttg tgcataatga aaccgaagaa ttacatcctc gcactttaga
     4741 attaattgaa gaagtggcga aacgtgtaga agaaaaaggc attcaagcgt ggtgggattt
     4801 agatcctgcg gaattgctcg gtgaagaagc caaagattac cgcaaagtgc cggatacctt
     4861 agacgtgtgg tttgattcag gatcgaccta tgcctctgtt gtagaacaac gccctgaatt
     4921 taacggcaat tcagcggata tgtatttaga aggttcagac caacatcgtg gttggtttat
     4981 gtcatcttta atgctttcca ctgctactaa cggcaaagca ccatacaaac aagtgctaac
     5041 tcacggtttt accgtagatg agaaaggcag aaaaatgtcc aaatcgcttg gcaacgtaat
     5101 tgtgccaagt gaagtgtgga acaaaaacgg tgcagatatt ttacgcttat gggttgcctc
     5161 caccgactat accggtgaga ttaccgtttc gcacaatatt ttaaacagtg ccggggaatc
     5221 ataccgccgt attcgtaaca cagcccgttt cttattggcg aacttgaacg gtttcgatcc
     5281 aaaaagtgat ttagttcaac cgcaagagat gatcgcctta gaccgctggg cggtggcttg
     5341 tgctttagag gctcaaaacg acatcaaaga agcctatgat aactaccaat tccacacggt
     5401 agtacagcgt ttaatgcgtt tctgctcgat tgaaatgggg tctttctact tagatattat
     5461 caaagaccgc caatacacca ccaaagccga cagccttgcc cgccgtagct gccaaacagc
     5521 gttatggcat attgcagaag cgttagtacg ttggattgca ccgattttat ccttcacagc
     5581 cgatgaaatt tggggctact taccgcaggt ggaaggcaga agtgaatttg tctttaccga
     5641 agaattctac accggattat tcggtttaac cgagcaagat aaattagatg atgcttactg
     5701 gcaacaaatc ttaaaagtgc gtgcggaagt caaccgtgtg ctggaacaag cccgtaaaga
     5761 caaagtaatt ggtgctggct tagaagccaa ggtgacggta ttcgccaatg atgaaattcg
     5821 cccgttatta gaacaacttg gcaatgaatt acgctttgtg ctgattacct cacaagcgat
     5881 tgttaaacca ctggcggaag cagatattgc ggaaggcgaa ttagccggac tcgcagtaaa
     5941 agtggaaaat gcagatggtg agaaatgccc tcgttgctgg cattatgcta ccgacatcgg
     6001 ctcgcactct ggccacgaag aagtttgcgg acgttgtgtg gaaaacgtag ccggcgaagg
     6061 cgaaaaacgc ttgtttgctt aaaatgtaat tcaattcatc tttctaaggc agaggaaact
     6121 ctgccttagc ttttccagcg atagatagac actcagttta aaatttgcaa aaattttcgg
     6181 aaaaataacc gcttgtattg atgtaaatga tgaaaataat cgcttttctt tttatttttc
     6241 tctccgtttt ttctcacact gctttggcta attctatggc tgaacaatgt gtcgcgttaa
     6301 aatctaactc gccggaaaaa acggctattt ataaagctga atgggttgag aaatctgctg
     6361 agttgccggc ttactgttta gtacaaggtg aaattggaaa acgggtggag gtggataatg
     6421 ttccttacgg tattcaattt gagctacgtt tacccgaaca atggaatgaa aaattcttat
     6481 ttcagggggc gggcggtgta ggtggggtga ttttccctgc cattggtaag ctctatccgc
     6541 acggggcgag tggtgcaaat ggcttaaaca gaggctatgc agtagtgagt aacgacagtg
     6601 gtcacgccac tcgagattta gtgtttaccg cagatcccga agctcgctca aattatgcct
     6661 atgcctctat cggcaaagtg accacggttg ccaagcagtt gattgccgaa ttttaccgaa
     6721 aaccgagcaa gcacaattac attatgggct gctcgaatgg tggtcgtgag gcgatgatgg
     6781 cggcaatgcg ttatccacag gaatttgacg gtgtgattgc aggcagcccc ggctttcggg
     6841 tttcccgttc agtcttggcg gaagtatggg ataatcgagc cttgttggca gttgctccga
     6901 agaatggtga tggcgacaaa attttatccc aagccctcac tcaacaagat ttagatgtga
     6961 ttgccaatgg cgtattaaca cgttgcgata agttagacgg cttagcagac ggtttaatta
     7021 atgcgtggga acagtgtgat ttccagccgg aaatggtggc aaagcaactg gggcagaaaa
     7081 aagtcgcttt aatcaaaacg attttcgagg gggcgaaaaa cagtcgaggc gagctgattt
     7141 atagcccttg ggcgtatgat tccggcatta acgctaaagg ctggcgggat tggaaattag
     7201 gtgatagcca aaccgctcag cccaacagca ttagttttaa aatgggattg aaaagtttga
     7261 cccattacta tttagccccc agagtgccga gccgtgatcc actcagcgta gatttggatc
     7321 aagccagcga acaagtgaaa gcggtaggga gcattcacga tacagatcaa gttgatttat
     7381 ccgcttttaa gcagaacggt ggcaaaatgc tgatttatca aggcgtttca gacccgattt
     7441 tttcggcagt tgatctcaaa aattggtatc aaagcctaca acaagcggtc gaaaatccgc
     7501 aacattttgc aaaattgttt ttcgtgtcgg caatgaacca ttgtgggcga ggggcaacgg
     7561 tgaatgattt tgatatgctc actgcccttg aaaattgggt tgagaaagga ctggagccgg
     7621 aaagtattgc cgccaaagcc ggtgagcttt accctaataa agccaagcag atcccgcttt
     7681 gtgcttatcc gaaagtcgca atttatcaaa ccggagaaga tcctaatcgt ttgaccagct
     7741 atcaatgccg ttaagcgaaa atcgctttat tggcgatttc agtgtaaaat agcaggcaac
     7801 gattgaaaat tttggaatca atgatgttaa ttttgaacaa acaacgtaaa gctgaatacc
     7861 tattgaaaag cctgccatac ttaccacaag tggcagagca ggttcagttt ctcgcctcga
     7921 gcaaagcgtt taaacaacaa attttacagc tgattcaaac ggcaaaaaag cgtatttata
     7981 taaccgctct ttactttgaa aaagatgaag ccgggcagga agtattagct gctttatatc
     8041 aagcgaagcg aaataatcct gaactggaaa ttaacatttt ggttgattgg caccgagccc
     8101 aacgtgggcg tattggtgag gcagagaatt tatctaacgc cgactggtat gcaaaagtta
     8161 aatacgaaca aggtttaccg gcagggcaag agattgcctt ttacggtgtg tcgattaacg
     8221 gacgagaagt gtttggtgta ttgcatttga aaggcttcgt atttgacgat acgattttgt
     8281 atagcggagc aagcattaat aatgtgtatt tgcatcaatt tgaacgttac cgttatgacc
     8341 gttaccacgt cattgaaaat aaagttcttg ccgatagctg ggtgacgttt attcaacagc
     8401 atcttttaac caatgaagcg gtaaaacgtt tagatacgga aaatcgcccg aaaacggctg
     8461 aaattcgtgc gcaaattaag catttccgca aacaattaag tcgggaagcg tatttattta
     8521 acggtgttgc tgaaaatggt cgcttagcgg tttctccttt agtaggctta gggcgacgta
     8581 agaatcagtt aaataaagtg attgaagcgt tgttttatca aactgagcag aaattaacga
     8641 tttgcactcc gtattttaat tttccgcatt ctctgagaac ccgtattgaa tggctattgg
     8701 caaacggcaa atcggttgaa attattgtgg gggataaaac cgctaatgat ttctacacca
     8761 agccggacga aaaattcacg atggcatcgg ctctgcctta tttgtatgaa aagaacttac
     8821 gagcctttgc aaaacggttg gacggttatg tacaaaataa acagttaacc attcgcttat
     8881 ggaaagatgg cgaaaacagc taccacttaa aaggggtttg ggtggataac cgctatattt
     8941 tgctgaccgg caataacctt aacccaagag cgtggcggtt agatgccgaa aatgcaattt
     9001 taatttccga tcctcaacat caattaagtg aaaaagcaga aacggagtta aaccaaattt
     9061 gccaacatac gcagatttta actcactaca gtgatttgga agttttaacc gattaccctg
     9121 aaaatgtgcg taaattgctg aaaaaattcg gcagggttaa gttggataaa attgtgaaga
     9181 tgttgcttta gttaggtatt tatatgggcg agctgatggg ttcgcccaat tattaaggat
     9241 aaaatatgag cgaacaaatt tatggcattc acgcagtgaa agcctttttg gataatgcac
     9301 ctgagcgttt aattgaagtg ctggtgctaa aagggcgtga agataaacgc ttaatgccac
     9361 tattaaatga attacaacgt ttaggggtgg caattcaaca agtgaaccgc caaaccttag
     9421 ataacaaatc gcaaggtgaa gtgcatcaag ggattattgc cagagtcgtg ccacaaaaag
     9481 agctgaatga acacgattta gaggcgatct taagccaaaa gaaaaatcca ctcttgttga
     9541 ttttagatgg cgtgaccgac cctcacaacc taggggcttg tttgcgtacc gctgatgcgg
     9601 caggggttga tgcggttatt gtgccgaaag ataaatctgc tcagcttaca ccaaccgctc
     9661 gcaaagtcgc ctgtggggca gcggaagtga tgccactgat tcgagttacc aatctggctc
     9721 gcacaatgcg agatttacaa gaacggcata atgtctggat tgtcggcacg gcaggtgagg
     9781 cggaatcggg catttatgaa gcaaaattaa ccggctcgat tgcgttagtt atgggggcag
     9841 aaggcgacgg aatgcgtcgc cttacccgtg agcattgcga ccagcttatc agcattccaa
     9901 tggcgggctc agtatcttct ttgaatgtct cagttgcaac cggggtctgt ctgtttgaaa
     9961 ttgtacggca gaagctggct gggcaataag gaataagtaa gaaaaatgcg aagaactgat
    10021 gtcttcgcat ttttgttttt agagccgcaa aaaatctcag aaattgaacc gcttgttagc
    10081 ccacatactc ttccaaatta atcactttat cgcacgattt ctcaattaga taaggatcgt
    10141 gactgaccaa aatcagactg caatttctcg ctcggcactg gttgatcagc aattctatcg
    10201 tttcttgttg tgtgattggg tcaagccgag aagtgacttc atcggcaaac agtagtaccg
    10261 gttctaagag caaggctcgc aaaattgcta ctcgttgtag ctcgccgccg gagacatttt
    10321 ccgcattgcg ttgcaaaatt tctgggttga gcgaaagttg ttgcagtaaa cttggaatcg
    10381 gcagtcgatc taattggtgc ttgttaatca catcatcaag caaggttttt agactgacat
    10441 tcggagcaaa ggcttccggt gggtcttggt aaagcttcaa cacttggtgt tttttatggc
    10501 tgtggctgtg ccacacaacc tcgcccgatt tcggcttaag caaaccacaa agtacatcgg
    10561 caagcgagct tttgccaatc ccactatgcc cgacaatccc aagcacttcg cctttgtgta
    10621 agtcaaagga aaggttgcta aacagcgttt tcttaccacg ggcaatactc aaatttttaa
    10681 ccgaaacaag cggttgtttt tggcttaaat tttgcagatc ttgcaatggt tgccaatgtt
    10741 ttggatcggc actgataagt gctttggtgt aagggtgttg tgggttattt aacaacgctt
    10801 ttgcctcacc ttgctccaac agttcgcctt ttttcatcac taaaatagtg ccaccgagtt
    10861 gctcggcaac ttcaatatcg tgagtaatgg tgagcaagcc tccaccgttt tggtaggcgg
    10921 attttaacag ttcaatcact ttgcctttac tgattgggtc aagccctttg gtcggctcat
    10981 cggcaagtaa aattttgcca ccggcggcag ttgcaatcgc aaaagaggca cgttgagcca
    11041 ttccaccgga aagctggtgt gggtagtttt caaccgattc gcccagccca agtttggcta
    11101 aataggcttt gctttccgtt ttggcttgag atgatggttt gcctgccaca tagtgaaaac
    11161 tttcccacaa ctgcttgccg attgccataa tcggattaag cgagcggcga ggctcttgtg
    11221 gcagcattac caaggtttta ccccacaatg tttgcaagcg gtcggattcg gtggaaattt
    11281 tgcaattttt taccgaaatt tcaccgcttg cgattaagcc gtcgggtaac gcccccataa
    11341 tcgcttgaat cagcaggctt ttgcctgagc cggtttcgcc taagatagta atattttttc
    11401 ctgcctttaa ttgtaggcta atcggttcga ccagctgtaa tctgttttcg gtttcaacag
    11461 ataggttttg gatatcgagt aatttcatct tcattttatt actgtagggg cgggccctgt
    11521 gcccgcccat aaatataata agaattttgg gcaggcacag gacctgcccc tacgcattat
    11581 ttaatttttc tagacaataa ttgtaagctg agtaccatta agaaaacggc aaccactggt
    11641 tgcaaaaata tcagcggtgc ttcgtagtag taagggaata gttcggtcat catcaagcct
    11701 aattctgctg taggcgggcg aagccctacg ctgacaaagc caagtgtggc gagagctaaa
    11761 atcgcattgc cgagcgagaa tgccgataaa gtggcaatca tcggtaataa acggggcaat
    11821 aagtggcgtt tgaagctata aaaccagccc attcccatta ggcgtgaggc ttgaatttcc
    11881 gagctgtttg ccattgttga actgatagag cggatcacac ggaaaaattc cacccacatt
    11941 accaatgaaa tccctaaata gagcgtccaa aatgagccgg gagaaagggc ggcaaagagc
    12001 aatactaaaa gtaagccggg gagtgccatc actagatcac aaataaagct gaaaatgcgg
    12061 tcaatccagc caccgaaaaa gcctgccata atgccggtaa ttaagcctgc aattaaggca
    12121 caaatcacgc taaacagtga taagccaaac gaaagacgaa tagcagaggc aagacgggca
    12181 agcatatccc gcccgagatg atcggtgccg aaccaagcga tttcatttgg attggaaagg
    12241 atattggtta aatcttgaaa gccaatatcc atcgggtaaa aataaggctg taaaaaggca
    12301 aatcccaaca ttgcaaggag aatagtgccg cctaaatatt gtgaaattga aattttcatc
    12361 ttgatgtcct atcgcttaat tcgtgggtca atccaagcac ttaatagatc ggcaatggtg
    12421 tttaatacaa caaacaatcc gcccatcacg agcgttgtac cttgaatcat cggcacatca
    12481 cgggcaatga tggcgtgaac cagtgcgtgt ccgctgccgg gccaagcaaa taggctttcg
    12541 accaccacaa cgccttcaat taaataaact aattgtactg cgtggtaggc aatgaccggc
    12601 acggcaatat tcggcaaacc gtgacgtaaa aaggtatgcc aacggcttaa gccttttaat
    12661 tgtgaaaatt ggtaaaattc tgagcctttt acctgaacca ttgcagaacg ggtgactcgt
    12721 accgaaaccg tactcaagcc aatcgcaagc gttaaggcag gcaagataaa atgttgccaa
    12781 cagccgtatc cgccggcagg tagccatttt agttgagcgg aaaacagcgt aatcaagcca
    12841 atcgcaataa taaatgccgg cacggagcga aacagggtac tgaataccag tgtcaagcgg
    12901 tcaaaaaagc cgtttggctt acgggcggct aacaaaccga gcggaggccc gataaggatt
    12961 gctaagataa gtgccacaat cgcaaggctt aaggtatgcc cgaattggtg ctgaatttca
    13021 gaccagaccg gatcaccggt caccaatgat ttgcctaaat taaattgagc caagtcactt
    13081 agccagctga agtagctttg ccaccaaggt tggtctaatc ccagctcggc acgcacagcc
    13141 tcagcagcag cactatctgc ttgatcataa ccatagcggc tggcagcaat gcggtatgcc
    13201 atatcacccg aaagttggcg ggtgaggata aatgtgagtg tgccaaccga ccaaatcacc
    13261 agtagaattt ggataagacg gcggaagaga attttgtgca ttgtattatt tcatttatta
    13321 aaaagatgat tttatgcgtt ctgatccccc tctttagcaa agaggggaag ggaagatttg
    13381 gcagaagaaa taagcggaga aaataggtga aaaatgagaa aaaacgataa ttctgtcact
    13441 atttattcac ctaagagcgc aaattactac tgaaaaatcc taccttccct tttctaaagc
    13501 gaggagtatt ttgaattaaa ctacttactt aataactcca aaaagaagcg tctttcaaat
    13561 ggatctagcg tcacattttt tacttcttta tgtgccacca cattttgttg gtaataaacc
    13621 accggaatga ttggcaaatc attgtaaata attttgctaa ccgtttgctt taatgctttc
    13681 gctttttgcg ggtcacgctc ggtttcgagc gatttgactg cattgtctac ctcagtattt
    13741 tgccaattca ttacccccca atcactgcca ccattggcga agtcggaggc aattacgcca
    13801 tatggatcta aggttttgcc ataatttaag gcataaagag ccatttctaa actgccgtct
    13861 tgatgccccg ccggaatttc gctgaaatta cccaccgaaa cattcacttc cacgccaatt
    13921 tgtttccatt gagcttgtaa gatagtagca atcaccggta attcaggacg atctgagaag
    13981 gtgcgcatgg taaagctaaa cggtttgccg tctttggtta agcgaccttg ttcatcaaag
    14041 gtatagccgg aagcggtcag attttgttta atttttgcaa tatcgaccac ttgttctgcg
    14101 gtatcaacac gccagtcggc aaaggctttc ggtaaaattt gatcggcagc tccgttctca
    14161 atttttaaga cctgctcagc aatcgccttg cgattaatcg cttgcgataa ggcttggcga
    14221 atcgctaagt cgttaaaaaa cggctttgcc acgtccattt tcaactgaat agtacgggca
    14281 atagatccgc tgactaattg caggttagga tcggctttca gccttgctac actggcggta
    14341 tctaaattaa ataccagtga tgacggctcg ctttgtgcca ttaacatacg ggtttcgcta
    14401 cggctgctgg ctaaataatt ggcttgctga acttgcggtt ttttgcccca atagccctca
    14461 aattgcaccg cttccaattt ctgcggagct tcaatttttg tgggtttaaa cgcacctgta
    14521 ccgataattt gggtcacttc gcctttttca ttatagcttt caggtgccaa aattaaggcg
    14581 gtgtagtgtg ttaaaaaggg aggaaacggc acaaaagctt tagttagctc aatttccact
    14641 ttgttatcta ccgctcgaat ttccttgata aaggcttttg ctaacacacc cggtttgctt
    14701 tttgcgatat tcaaactatt taccactgct tgtgcggtaa gtacttgatc gttatggaat
    14761 tttacccctt cacgcaaggt aaaacgccag attgttccct cttcgttact gcgccagtca
    14821 gtggccaatg ccggaattaa attcccgtta agatcggctt cgaccaatgt ttccgctaag
    14881 ttcatacgct ggaaaattac gcctgattga ttcgggtcaa ggctattgat ttcccatggg
    14941 gcaaccacgg ttgcaagcgg taatatttcc gcattttttt gcgattgagt attctctact
    15001 tgcggttttt ctgcggttgc ttgagtgctg ttcttatcat cgcaagcaga aacacagaac
    15061 gctgcgacag ttaatggaat caaccatttt ttcattttat tttctccata aaaaaatcag
    15121 ggctatgaat attggcgaac tttagcatat ttccataaaa aaagtagagt tttctgcgag
    15181 ggtatttttg agtggttaat taataggcag aataatctca agataaggat tgttgatagg
    15241 ggaatattta ggctatttga ggaacagaat gcctagtatt gtaaaattcg gaaaaaaaat
    15301 tgaccgcttg tgctttattt gtaggggcga aagatttttc gcccctattg gttttagtgc
    15361 tctctcgttt taaagaactg tacgtcaggg taacgttctt gggcgaggtt taagttcacc
    15421 attgtcggag caatgtaggt gaggttgtct ccgccgtcta gggcaaggtt tgcctcgttt
    15481 ttgcgtttga actcttcaaa ttttttggca tctttgcctt cgacccaacg ggctgtggca
    15541 acgtttacgg tttcataaat tgcctctaca ccgtattcgg ttttcagccg tgaaaccacc
    15601 acatcaaact gtagcacccc aaccgctccc acgatcaaat cgttgttgat taacggacgg
    15661 aatacttgca ccgcaccctc ttcggaaagt tgtaccaagc ctttgagcag ttgtttctgt
    15721 ttgagcggat cacgcaggcg gattcggcgg aacagctcag gggcgaagtt cggaataccg
    15781 gtgaatttga aattttcgcc ttgggtgaaa gtatcaccaa tttggatcgt gccgtgattg
    15841 tgcaagccga taatatcgcc ggcgtaggct tcttcggctt gtgaacggtc gcccgccata
    15901 aaggttaagg catcggagat caccacatct ttgccgatac ggacgtgttt aagcttcatt
    15961 cctttttcat atttacctga aacgacacgc ataaaagcaa ctcgatctcg gtgtttcgga
    16021 tccatattcg cttgaatttt aaatacgaaa ccgctgaatt tttcctcgct tggtgaaact
    16081 tcacgttcgt ccgattgacg ggcttgtggt gccggtgccc attcggttaa accgtcaagg
    16141 aaatgattga ccccgaagtt acccagtgca gtgccaaaga aaaccggtgt cagctcgcct
    16201 ttgataaatt cggtgtgatc gaattcgtgc gaagcgcctt gtaccagctc taattcatca
    16261 cgcaactgct gggctaaatc atcgccaacg gcttgatcca gttccggatt atttaagcct
    16321 ttaaccactc gtacttcttg aatggtatga ccttgtccgg tttggtataa gtaggtttcg
    16381 tccttgtaaa ggtgatatac ccctttaaac aattttccgc aaccaatcgg ccaagtgatc
    16441 ggcgcacatt tgattttcag cacgctttcc acttcatcta acagctccat cgggtcacga
    16501 atatcacggt ctagcttgtt cataaaggtt aggattggcg tatcacgcaa gcgggtcact
    16561 tccattaatt taatggtgcg ttcctcaacc ccttttgccg agtcgataac cattaagcag
    16621 ctatctaccg cagtcaaagt acgataagta tcttccgaga agtcttcgtg ccccggtgta
    16681 tcgagtaagt ttaccaagca atcattgtac gggaactgca tcacggaggt ggtaatcgaa
    16741 attccacgtt gtttttccat ctccatccag tcggatttag cgtgttgtgc cgagcctttg
    16801 ccttttaccg agccggcagt ttggattgca tttccgtaga gcaatacttt ttcggtaatg
    16861 gtggttttac ccgcatcggg gtgggaaata atcgcaaagg tgcgacgttt attaacttct
    16921 tgtgggtatg acatttttct caaccaatta aaataaaaag ggcggaaagt ccgccaaaaa
    16981 attaagcgtt attttcgctg atttgaggcg agagggcaag cggttaaatt tggcggaaat
    17041 tttgcaaaat cggctagatt tgcaaaaatt ttgtaaaaat cgaccgcttg ctttggcaca
    17101 attttggaaa cagggtagaa taaccagctt tgactttatt atcgatgaat ctattatgat
    17161 gaaaaaattt attcctgtta tgattgcggc tctggtgtta agcgcctgtt cctcccagcc
    17221 ggcaaaaagg aaaagcacac cacggattaa agcaaccgct gcttatattc caaccgaaaa
    17281 ttccgcttat gtggtgtttg acttaaacca aaagaaattt ttggaaggta agaatttcaa
    17341 tttagttcgc ccgattgctt cggtcacaaa attaatgaca gcagtcgttt tcttagaaga
    17401 aaaccgctat ggggcgagtt gccaaaccca aattttgccg tcagatgctg atttcttaaa
    17461 aggcacgacc agcccattgc cgaaaaatct taacattgcc tgtagtgaat tgttaaaagc
    17521 gatgctagtt cgctccgata actatgccgc tcacgccctt gcccacgcaa cacatttgaa
    17581 taaagcccaa tttttggctc gaatgaacag caaagcccgt gaaatcggta tgactcaaac
    17641 ctactttggt gatagctcag gcttatcttc ggataatgtt tcaacggtgg ttgatcttgc
    17701 taaattggca ggctatgcgg caagtaagcc tgaaattcag caattgtcta attcgccatt
    17761 agtgagtgtt tatgcaggtg gtcgttattt ctctatgaaa aacaccaaca gtatggttcg
    17821 ttcccaatcc tatcaggcat tagtgagtaa gacaggttat acccgtgagg cgggttataa
    17881 tcttgccttt attgctaaag gggattgcag aggcaagcga attggtgtgg ttagtttaaa
    17941 caatgataac gctgcttcac gcaccgattt cactgaatcg atgttagcga agtatcaatg
    18001 cgtaccaaat ccgtatagac aatatgatta atggttgcct tgagaaaata gcggtttttc
    18061 tctaaaatac tgccttttaa attaaatgaa ttgagacaag attgtgtcag aaattaaatt
    18121 aattgtcggg ctggcgaacc cgggggtaaa atatgaagaa acccgccata atgcaggcga
    18181 gtggctgatt aacgaacttg cccgaatata taacgttagc ctaaaagaag agccgaaata
    18241 ttttggcaaa gtagcaaaaa ttaataccgc tcaaggcgaa gtccgtttat tagtgccgac
    18301 tacttttatg aatttaagtg gtaaagcagt gggagcgtta gccaattttt atcggattaa
    18361 accggaagaa attttggtcg ctcacgatga attggattta ccacccggtt cggtgaaatt
    18421 gaaaaaaggc ggcggacacg gtggtcataa cggcttgaaa gatattattg ccgcccttgg
    18481 caataataaa gagttttacc gtgtgcgaat tggtattgga cacccggggc ataaagactt
    18541 agtggcgaat tatgtgctgg gcaagccttc accaagcgat cagacattac tgaatagtgc
    18601 ggttgatgaa gccgcacgtt gcgtagaagt tttatttaaa gacggaatgg ttaaggcaac
    18661 taaccgttta aatagtttta aagcaaatta aaactctctc ccttgtggga gagagaccga
    18721 aaaatacttc gttcagaagt atttttcaga gagaggggag cggtcatatt tgaggaaatt
    18781 attgcaattt cgccctctcc ctcattttcg cttctgaacg aagctcaaat ttatccctct
    18841 cccgcaaggg gagaggggaa ttctaaggaa caaaaaatgg gatttaaatg tggtattgtg
    18901 ggtttaccaa atgtcggtaa atcgaccctt ttcaatgcat taactaaagc aggtattgag
    18961 gcagcaaact atccgttctg tacgattgag ccaaataccg gtgtggtacc aatgcctgac
    19021 cctcgcttag atgcgttggc ggaaattgtg aaacctgagc gagtgttacc aactacaatg
    19081 gaatttgtgg atattgccgg cttagtggca ggtgcaagca aaggcgaagg tttaggtaat
    19141 aaattcttag ccaatattcg tgaaactgat gcgattggcc acgttgtgcg ttgttttgaa
    19201 aatgatgaca tcgttcacgt tgcggggaaa attgacccag ctgatgatat tgaaatcatc
    19261 aataccgaat tggcacttgc cgatttagac agttgcgagc gtgccattca gcgtttacaa
    19321 aaacgtgcga aaggtggcga taaagacgca aaatttgaat tatcgattat ggaaaaaatc
    19381 ctacctgtgc ttgaaaatgc cggtatgatt cgttcggtag atttagataa ggatgaactg
    19441 cacgctatta agggctacaa cttcttaact ttaaaaccga caatgtatat tgccaacgtg
    19501 aacgaagacg gttttgaaaa taacccttat cttgaccgtg tgcgtgaaat tgccgaaaaa
    19561 gaaggggcgg tggttgtacc ggtgtgtgca gcgattgaat cggaaattgc cgagcttgat
    19621 gacgaagaaa aagtggaatt tctacaagat ttaggcattg aagagccggg cttaaaccgt
    19681 gttattcgtg caggctaccg tttacttaac ttacaaacct actttaccgc aggggtaaaa
    19741 gaagtgcgtg cgtggacggt ttcggtaggt gcaaccgctc caaaagcagc agccgtgatt
    19801 cacactgact ttgagaaagg ctttattcgt gcggaagtaa ttgcttacca agacttcata
    19861 gacaataaag gtgaagccgg tgcaaaagaa gcgggtaaat ggcgtttgga aggtaaagat
    19921 tatattgttc aagacggcga tgtaatgcac ttccgtttca atgtttaatt tgcaaatttt
    19981 tgcctaaaaa agaccgcttg ttgatctgtg atctacaagc ggcctttttt tgttaattaa
    20041 ttgcatattg ctcgattcgg taaaaatgtc tt
//
LOCUS       DANLXS010000022        19945 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.022, whole genome shotgun sequence.
ACCESSION   DANLXS010000022 DANLXS010000000
VERSION     DANLXS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19945
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.022"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..642
                     /locus_tag="PXD71_000647"
     CDS             <1..642
                     /locus_tag="PXD71_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2572 family protein"
                     /protein_id="HDL6290802.1"
                     /translation="ILISTILSVLFLSKEKWLGQQNITHFYREKYLSDQYNFLALYPQ
                     DKTQICQKFQKEQISQADLSDKQFRFLQYQFACQFHSLFKDKKPTKEKYIHFTHLADY
                     LDLSNVPNQEIYTIHSLAELPSSSEREPKIVLAKNAINENLPQEFYGIVITDYLFDIT
                     GKKMYGTLYSSYDNAREERNLTFKKEVLAELEARYSAWRYLPYSEHLMGAVDD"
     gene            635..928
                     /locus_tag="PXD71_000648"
     CDS             635..928
                     /locus_tag="PXD71_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5374 domain-containing protein"
                     /protein_id="HDL6290803.1"
                     /translation="MIKYKAETALSLLVAITLFAIVVLSFSQWQSAQNHRLNQHFQQQ
                     QAVMILENQLALKLAGLDCESSITQNQIRYQIQCSGNRLTIRFPLGKIELNND"
     gene            977..4267
                     /gene="recC"
                     /locus_tag="PXD71_000649"
     CDS             977..4267
                     /gene="recC"
                     /locus_tag="PXD71_000649"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDL6290804.1"
                     /translation="MFTLYQSNQISSLAEMLVKIQQVNPLEDPFEPETILIQSQGMAQ
                     WLQMQIAELNGVMGNCDFLYPTTFLWQQYRLLFPELPKENIFERSSLVWRIMRLLPSC
                     LTLSEFAPLKRYLGDQPNKEQYQLKLYQLSAKIAELFDQYLVYRPHWLVSWEEGNLQA
                     IENEIKIATAAKPIDFDLIRADMKWQSHLWQQIVSDLQSESDEQVFTTSHRAYLQQNY
                     FAKLDDLTETEKAKLPKRIFIFGISSFSPLQLAVFKKLSEHCDIHLFFFNPSEKYWGD
                     SVEEKVWQKLALSHKISQDDLDNLLAEQRNKLLNLWGKQGRDFLAQLIEFEPNTMDVF
                     VEPEENSNLNKLKKQIFSLENDATITDLTADNSLQIHSCHSPMREVEVLHNQLLNLFE
                     QDSTLLPKDIIVMSPDINKYAPYIDAVFSRYNTGNRDPRYIPFSLSDQAFTQVDPIIH
                     SFLQLLAMKESPFNAEELFDLLEVNAIQTRFGFSTEDIHTLRVWVRNAGVRSGVEIEQ
                     PHWQNYNSWENGLNRLLLGSSLKEEQGIWENTLAFGDSYGLNAELVGRLASFIEALLA
                     WVNFLQTPQPLAAWQQAIKNLTADLYQENAENAASILLLNNVNDQIIEQISATHFSDD
                     LQIEVLALLFEQQLSQHSQQLNFLVGRVNFATLLPMRAIPFKVICLLGMNEADFPRQQ
                     QINSFDLMQYAPKKGDRAKRDDDRYLFLEALLSAQQMFYISYIGQSQKDGKEMLPSIL
                     VSQLLDAMNEALTPECKQQLSDEVEVLVRKQPLSVFSKKNFTHPRDHAYNAEWIIKPT
                     VAVQDFLSEPLTNESVASLELDELISFVQNPIRFFFLKVLGISFNTYDDTIDESEIFE
                     MSGLDNYRILNELLTINLAQQQRFFAEEQLKGNLPASYFAQIATQDLIGKITEMQAAL
                     SHYLASESRILSVNREVELERQTLTLNGNIANLYQNEVVLWRVGHLRDKDKIQTWIYH
                     LLLCAYFPEKTVKFYYREGEQVGVLSFETVSQIEALSQLSIYLNAYLDGLSQAQLVIY
                     DRIEEYLKGDGTNSEQVLIDLAEKDERDGSYLHRLLAQTKELDSQAIHQRTQSWFSLM
                     NQKIAKGD"
     gene            4403..7348
                     /gene="ptrA"
                     /locus_tag="PXD71_000650"
     CDS             4403..7348
                     /gene="ptrA"
                     /locus_tag="PXD71_000650"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDL6290805.1"
                     /translation="MKLSFLTQFVAAAFALNVMTYPAFAEVAKNSEKTTASQPQSQLG
                     FELIKQSINKSPNDKAIYQGIKLDNGMEVLLISDSQANKSLMSAIIPIGSMDDPISQQ
                     GLAHYLEHMILMGSKNYPETNSLDGFLTKNGGMNNASTASHRTAYYLQVNNEAFSEAV
                     TRLADTLAFPLLLETNAKKEVNAVNAEMVRAKSHDGHLLHSVNLATANPAHPATKFAV
                     GNKETLSDKPDSKLQTELEQFYKQHYSANLFKVVLYSNQSIEQMAKLAANTLGKMENK
                     HFSIPQTAEPRYRDEDKSVFIQYKPVKSNKMLVIGFDFPNDEMQFKHKTGKYIAYMLN
                     NNTDGTLSDYLIKQGLSDGGIEAVSSANIGRDRGSFDIYIELTDKGLSEQDQIISLVF
                     QQIEKIKQEGIQESYFSEVKESLKQDFQHLQVEKNMDFVEFIADQMLHFPLEHVIDQP
                     YLTETMDNEAIKAKLAAMNIDNARIMLVSDTAKTDKKTPYFEAGYSVAKITQEQKQKW
                     LDFSKNPQLNLPELNPYFTTDFTLNKVETGLTKPKLINDQAGNLIYTMPSVYFTNEPK
                     AKIGVGFNIRPRKDDLKSLTAASILGYMNNLAQTKLDFQSSVAGMNLSLAPAANGLAL
                     SAEGYTQNLAKLMLDSVKQFSRFEFNEDVLAQAKQRLHQELDRNEKENSLNQANAALS
                     RFASYPYFDVPKQREMIDSITLADIEQLRQRILQQSSGFKLLSVGNLSAEQVSALAED
                     LQAVIKNQKSANATGQYVDVSTSQRKINVIKSVPNEDNALLMAFMPNGYNELESLTRA
                     KLLRSIISRWYFDDLRTDKQLGYVVYATDNIIGKTAGIQFMVQSPNTTPAGILEHNER
                     FFTQSFERLKNLSDEEFGKYRDSLVEKLQYKPESLAEEFSEFGSDFSRNNPKFDRLQQ
                     VIELTKALTKADIVAFYQKAVIEKQGFVFVSQAVGTKTKAEDVAKFDGFEQIESIEKL
                     QREFEIKDY"
     gene            complement(7397..9043)
                     /locus_tag="PXD71_000651"
     CDS             complement(7397..9043)
                     /locus_tag="PXD71_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6290806.1"
                     /translation="MKFPTLTKISASVLAVLALAACDDKSNENKPTAAAAKPQTEKTF
                     VNCVSRSPTGFSPILMMDGLSYNASSQQIYNRLVEFVPGTTDIEPALAESWEISEDGL
                     TYTFKLRKGVKFHSNKDFTPSRELNADDVVFSFNRQLDKNHPYHTVSNGTYPYFNAMK
                     FPTLLKSVEKVDDSTVRITLTKRDASFLSSLGMDFTSIYSAEYADKMMKAGIPEAVDT
                     TPIGTGPFVFSGYVLDQASRYVANKEYWKGKPDIDRLIFEIVPDATTRYAKLQSGQCD
                     LMDFPNATDIEKMKNDPKINLLSQPGLNIAYVAFNTEKAPFDNVKVRQALNLAVDKKA
                     IIDVVYQGNGFAAKNPLPPTIWGYNDSLADSEYNVEKAKQLLAEAGFPNGFETELWVQ
                     PVVRASNPNPRRMSEIIQADWEKIGVKAKLVTYEWGDYIKRTKAGELTAGTYGWSGDN
                     GDPDNFLSPLFGTPNIGNSNYARFTNPELDALLEKALGSSDKAERTKLYEQAQVILRE
                     QAPWINVAHSINFAPTSKRVEGYKQSPFGYTYLYPVKIVD"
     gene            9119..11209
                     /locus_tag="PXD71_000652"
     CDS             9119..11209
                     /locus_tag="PXD71_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL6290807.1"
                     /translation="MIFANQSIELDTVNVIATRDPSKFADTPQKQTKDALLVKQATSV
                     AAALKALPNVDVQGGARAIVQKPSIRGLSDNRIVQVIDGVRQNFDLAHRGSYFVPMSL
                     IQEIEVIKGPSSSLWGSGALGGVVAMRTPNALDLLKNNDKFGAKIRQGYQTANNLSET
                     EASVFAANDRFDALLSGFYNNADNLRSGEGKELNNTGYKQAGGLVKFGWQINDTHRLE
                     LSHRHSQFKQTAPGNNEVENQFTDNDVSAQISAWHAANPGNPSNPVYLAKMLAFYGQL
                     HSSLGSVSYLSDQKITDKSTSLNYYFNPSNPYLSTQVTLYSNSTTENEHRKISGVEDQ
                     TKLSTRGVNIRNHSELSWFSLVYGVDYMLDKVATQRGRNNTDAQYRANPYDAKSKTTG
                     AYLIAHIPLFDEKVVFSPSVRYDRFDTSSETVKYKDSHWSPAAKLTWKATNWLDLTAK
                     YNEAFRAPSMQERFVSGSHFGTTVRGLPINNIFLTNPNLRPETAKNKEVTFNVHFDNV
                     ISQGDKFNFEATYFRNDVKDLINLQVFKANPTSPMEFIPTRSQYQNIANARLSGIELQ
                     TRYQTERLAVFANYGSTKGKDKDSGEALSNIAASKIGFGVNYAVVQDKFTVGANVTRY
                     QAQHRVPKNHGVTYQGYTLTDLHATYAPLKGEWKNLRLDVAVENLFDKKYQPAFSLME
                     GSGRNVKLSAAYSF"
     gene            11280..12893
                     /locus_tag="PXD71_000653"
     CDS             11280..12893
                     /locus_tag="PXD71_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL6290808.1"
                     /translation="MKFYPSLIASAVSFAVVLSGQAAVNVPDAGSLQRELDQRQAQQK
                     VKPSGEWFNQAAKAPYTATDGQELTFPLTQVQVFDRTNQEISTPELAPILANYVGKAV
                     SLTDLSNLANEITEFYRSHNYLVAKAILPPQEIEQGKVKILLFKGNVGEVRLQNQSAL
                     SDKFVARLANTTVNGSEFILKDELEKFALTMNDVPGVNTGLELSAGKQAGEANLLIKL
                     QNAERFSGYVSADNQGNKNTGRYRLATGIKANNLIGWGDELKVDLMSSNNANLKNARL
                     DYSSLIDGYATRLGLTANYLDYELGGNFKALQSQGHTHNLGAYVLHPTIRLPDFRLNT
                     KVSFNHQRLTDKQQAVNVAQKRQVNSLTVGINGSWNSLKDGTSYFSLSALFGNLANHT
                     NEQALPKSFAFNLGVNGQFADKTLDSSQKMLLGGLYGVRGHQAGVASVDEGHLVQTEL
                     KHYLPLFSKSLLTSSVFYDYGWGKYYKHSGFLARGVNNSVKLQSIGAGLSLSEAGSYA
                     INASVAKPLGKKIENADKHQFWFSAIKTF"
     gene            12905..15721
                     /locus_tag="PXD71_000654"
     CDS             12905..15721
                     /locus_tag="PXD71_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HDL6290809.1"
                     /translation="MFKRNAISLCILTACSGLAYANTADLPQGERVRVGTATIERTTD
                     KMTVNQSSNKVQIDWNSFDIGKNKEVEFKQPTEMAVAYNRVTGGNASQIQGKLTANGK
                     VYLANPNGVLITKDAEVNVGGLLVTTKDLEKISENGTKDKFSRKLAKEGKILNQGKIT
                     AKGERAFVVLVGDDVANEGEINAKNYYHKETKKVTECYDDSWSLVKQCYDFDKEIVKS
                     TSGEVHLSSGENVTFTLSDRYIDVALDDNTVNSIIKNDGAIIANGDITLTAKGRNQAL
                     DSLIINNGVLQANKVQNKNGTIILSAGETQLHEKSQIKGEKVSFAEASYYGPTEDIKV
                     VSKTGSKVTAPVIDFKGKTVNIQGEFGREDSTDLYNDELQILKTTVNIEVPNTEDIRI
                     ADTDNGGSGSFIQAGALASLLANNGRVNVQGKGFDVSGKLNVNAFKQTDSVLKFINQA
                     GINIHNADIHANGRLFFVTNLPNDTDFQSNIRITDSKINLGNGSMGFGRSSKRWEMHD
                     VVRRADTELRKKFNLDMKNVELNQVDDFVVAGGFQQVNLDNVKARGKTNFYLDSGNSR
                     MFVGEQIYEYGVTDIEKRTQLANLDQRRKRWNSDNYDETDLNWEYNQRFDGFAAKHNH
                     RAPIADTEINIANSDISLDNGFVHLMAEKINLDDSKINITFNQDNSLDESTQINRLGL
                     NGKVSMKNSHINVVGDEKDGISPARHYATMFLVGELLGEKSSIFLKSHQGAVLRTDSN
                     VTIAGKNSKDDLKITAITTGKKMGNEIIGPDGLPTSEDNDANASNTAFAIGYEPNATA
                     IVENASITALAPNGGLAYTGTSKTQIDAKDNATFTFFEKPRAGYDKLLGDIKGNPDKL
                     TPRDFARLYDNIKGIKASSLSEQQLGLADTAKKTSEHTLANSLDVENLVSVTVCDEQN
                     KCEERMLGNKNTNAKVIVGDIKFE"
     gene            15844..15975
                     /locus_tag="PXD71_000655"
                     /pseudo
     CDS             15844..15975
                     /locus_tag="PXD71_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(16030..16791)
                     /locus_tag="PXD71_000656"
     CDS             complement(16030..16791)
                     /locus_tag="PXD71_000656"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL6290810.1"
                     /translation="MKLNQAQIQNALICGQAVVFDEIDSTNEYLLTHHQRLENGTVCL
                     AEKQTAGRGRRGRTWYSPESENLYFSILWHYPMEQAADLPPLSLVVALIIAESLTVQN
                     VKDIQIKWPNDIYYQGKKMGGILLETQANRNGLDVVIGIGLNLGMAKVDENIVTQAWA
                     DLSQYHFNRNELVCRLAYELQKNLKIYPLVGFAHYAERWQSFDLFRHKAVKLITEAEE
                     IHGISQGINEQGELILKQGDNLRYFGIGEISLRAD"
     gene            complement(16803..17834)
                     /gene="murB"
                     /locus_tag="PXD71_000657"
     CDS             complement(16803..17834)
                     /gene="murB"
                     /locus_tag="PXD71_000657"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL6290811.1"
                     /translation="MMKHSLTPFHTFHLPAKATQIIEFTTVEQLLSEWQKAFNAQLPI
                     LILGQGSNVLFLEDFDGVVLVNKLKGITHHEDEHFHYLHVQGGENWHELVKWTLAHNI
                     AGIENLALIPGVASSAPIQNIGAYGVEFEQVCDFVEVLNLRSGELFKLSKSECEFGYR
                     ESVFKHQYRDEFAIISVGLKLAKAWKPVLSYGSLAQLNPETVTPIQIFDEVCAVRSSK
                     LPNPDEFGNAGSFFKNPVISNEQFAPIQAKFPAIPHYPQADGTVKLAAGWLIDQTGLK
                     DLQIGGAAVHTQQALVLINKENATGQDVLALAKTVRQRVKEKFGVEIQPEVRFIGKNG
                     EVDSEKITR"
     gene            complement(17938..18612)
                     /gene="crp"
                     /locus_tag="PXD71_000658"
     CDS             complement(17938..18612)
                     /gene="crp"
                     /locus_tag="PXD71_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDL6290812.1"
                     /translation="MQDMTLSTAPLENVTPPPSIHDPAVEWFLTHCHIHRYPAKYTLI
                     HAGEDAESLFYIVNGSVSVYIKDDETKEMLLTNLGTGEFVGELSLFEEKVQQRTAYVR
                     TKGPCEIAEISYKKFKQLVHLNPDILMYLSAQMARRLRQTSRQVGNLAFLDVAGRIAQ
                     TLLNLAKMPDAMTHPEGMQIKITRQEIGQMVGCSRETVGRIIKMLEDEGLISAHGKTI
                     VVYGAR"
     gene            complement(18674..18892)
                     /locus_tag="PXD71_000659"
     CDS             complement(18674..18892)
                     /locus_tag="PXD71_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDL6290813.1"
                     /translation="MIIPWQELEESTLHNILDSFILREGTDYGERELSLAEKRERLLT
                     QLKADKVVIVWSELHESLDIKDKKSFLG"
     gene            complement(18895..19500)
                     /gene="slmA"
                     /locus_tag="PXD71_000660"
     CDS             complement(18895..19500)
                     /gene="slmA"
                     /locus_tag="PXD71_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDL6290814.1"
                     /translation="MTEPKIKMPKKSVVERRQQVLEVLISLLNSEEGMQRITTERLAA
                     AVGVSEGALYRYFPSKTKMFEALIEKIEQTLTSYIHANKRQDSSANSVRAILYVILEF
                     ARKNPGVTRILTGHALMFEEELLKARVAKFFDNLELQFANILQLSKLRERKSFEDERA
                     LAGYLVNFCEGQFLRLVRSNFSYNQHQHFEKQWAFIKPLFD"
     gene            complement(19512..>19945)
                     /gene="dut"
                     /locus_tag="PXD71_000661"
     CDS             complement(19512..>19945)
                     /gene="dut"
                     /locus_tag="PXD71_000661"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL6290815.1"
                     /translation="LDSRIGNEFPLPAYATEGSAGLDLRAMLNEPLTVKAGETVLIPT
                     GISIYIADPNLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKVSLWNRSQADFTV
                     NVGDRIAQLVFVPVVQASFNIVQEFEQTDRGEGGFGHSGKQ"
BASE COUNT         5983 a         4150 c         4355 g         5457 t
ORIGIN      
        1 atacttattt ctacaatttt gtcggtttta tttttaagta aagaaaaatg gcttggacaa
       61 caaaacatta cccattttta tagagagaaa tacctttccg atcaatataa ttttttagcg
      121 ttatatccac aagataaaac gcaaatatgc caaaaatttc aaaaagagca gatcagccaa
      181 gcggatttat ccgataaaca attcaggttt ttacagtatc aatttgcctg ccaattccat
      241 agtttattta aagataaaaa accgactaaa gagaaatata ttcactttac ccatttggcg
      301 gattatttag atttatcaaa tgtgccaaac caagagattt atactattca ttctttagct
      361 gaattaccta gtagcagtga acgtgagcct aaaattgtgc ttgctaaaaa cgcaattaat
      421 gaaaacttac cgcaagagtt ttacggcatt gtgataaccg actatctgtt tgatattacc
      481 ggcaaaaaaa tgtacggcac gctttattcc tcttatgata atgcccgaga ggagcgaaat
      541 ttaacgttta aaaaagaggt actggcagag cttgaggcga ggtattccgc ttggcgatat
      601 ttgccatatt ccgaacattt gatgggagcg gttgatgatt aaatataaag cagaaacggc
      661 tttgtcgctt ttggttgcca tcacgctatt tgcgattgtg gtgttgagtt tcagccagtg
      721 gcaatcggca caaaaccaca ggctaaatca acatttccaa cagcaacaag cggttatgat
      781 tttagaaaat cagcttgccc tcaaattagc cgggttagat tgtgaatcaa gtattactca
      841 aaatcagatt cgctatcaga ttcaatgctc tggcaatcgc ttaaccattc gctttccgtt
      901 aggcaaaatt gagctgaata atgattgaat cgtccctcgc tttggaggac aataattcaa
      961 ttagcgaggc gttattatgt ttacacttta ccaatccaac caaatttcat cactggcgga
     1021 gatgctggtg aaaatccagc aggtaaaccc acttgaagat ccgtttgagc cggaaaccat
     1081 tttgatccaa agtcagggaa tggcacagtg gctacaaatg cagattgctg aattaaacgg
     1141 tgtgatgggg aattgcgatt ttctctaccc tactacattt ttatggcaac agtatcggct
     1201 gttgttcccg gagctgccga aagagaatat ttttgagcgt agttcgttgg tttggcggat
     1261 tatgcggtta ttgccgagtt gcttgacact atcggagttt gctccgctaa agcgttattt
     1321 aggtgatcaa cctaataaag aacagtatca gcttaagctc tatcaacttt ctgccaaaat
     1381 tgccgaattg ttcgaccaat atttggtgta tcgcccccat tggctggtta gctgggaaga
     1441 gggcaactta caagcgatcg aaaatgagat aaaaattgca actgccgcta agccgattga
     1501 ttttgactta attcgagccg atatgaaatg gcaaagccat ttatggcaac agattgtatc
     1561 tgatctgcaa tcggaatctg atgagcaggt ttttactacc tcccaccgtg cctatttgca
     1621 acaaaattac ttcgctaagt tagatgacct tactgaaacg gaaaaagcca aattgccaaa
     1681 acgcattttc atttttggga tttcgtcctt ctcgccatta cagttagcgg tgtttaagaa
     1741 actcagcgag cattgcgata ttcacctgtt cttttttaac ccaagcgaaa aatattgggg
     1801 cgacagtgtg gaagaaaaag tgtggcaaaa attggcgtta agccacaaga tttcgcaaga
     1861 cgatctggat aacttattgg cggagcaaag aaataaacta ctgaatcttt ggggcaaaca
     1921 agggcgggat ttcttagctc aattaatcga atttgagccg aataccatgg atgtctttgt
     1981 cgaaccagag gaaaacagca acctcaataa actcaaaaaa cagattttca gcttagaaaa
     2041 tgacgccact attaccgatt taaccgccga taattcactc caaattcatt cgtgccacag
     2101 cccaatgcgg gaagtggaag tgttgcataa ccagctcctg aatttgtttg agcaagatag
     2161 cactttgctg ccgaaagata ttattgtgat gtcgccggat attaataaat acgctcctta
     2221 cattgatgcg gtgttttctc gctacaatac gggtaatcgg gatcctcgct atattccttt
     2281 ttcattatcc gaccaagcat ttacgcaggt tgatccgatt atccacagtt tcctgcaact
     2341 gctggcaatg aaagaaagcc cgtttaatgc cgaagaactg tttgatttac tggaagtgaa
     2401 tgcgatccaa acccgttttg gctttagtac ggaagatatt cataccctgc gggtttgggt
     2461 gagaaatgcc ggcgtacgtt cgggtgtaga aatcgagcaa cctcactggc aaaactacaa
     2521 ttcgtgggaa aacgggctaa accgtttatt gcttggctca agcctgaaag aagagcaggg
     2581 gatttgggaa aacacccttg cctttgggga tagctatggc ttaaatgccg agctggtggg
     2641 gcgacttgcc tcttttattg aggctttatt agcgtgggta aactttctgc aaacgccaca
     2701 gccgttggca gcgtggcagc aggcaataaa aaatttgact gctgatctgt atcaagaaaa
     2761 tgccgaaaat gctgcatcaa ttttattgtt gaataatgtg aatgatcaga ttatcgagca
     2821 aatttccgca acccattttt cagacgatct gcaaattgag gttttagcct tattgtttga
     2881 gcagcagctc agccaacatt cacagcagct taatttcttg gtcggacggg tgaattttgc
     2941 caccttgctt ccgatgcggg cgattccgtt taaggtaatt tgcttgctgg gtatgaatga
     3001 ggcagatttc cctcgccaac aacaaatcaa tagctttgat ttaatgcagt acgccccgaa
     3061 aaagggagac cgtgccaaac gtgatgatga ccgctacttg ttcttagaag ccttactttc
     3121 cgctcagcaa atgttttaca tcagctatat cgggcagtcg caaaaagacg gcaaagagat
     3181 gctaccgtcg attttggttt cccaactgtt agatgcaatg aatgaagctt taacgcctga
     3241 atgcaagcaa caattaagcg atgaagtgga agttttggtc agaaaacaac ctttaagtgt
     3301 gtttagcaaa aagaatttta cccacccacg agatcacgct tataacgcag agtggattat
     3361 caagccgact gttgccgtac aagattttct tagtgagcct ttaaccaatg aaagcgtggc
     3421 ttcgctagaa cttgatgaat taatctcttt tgtacaaaac ccgattaggt ttttctttct
     3481 aaaagtgttg gggatttcgt ttaacactta tgatgacacc attgatgaaa gcgaaatttt
     3541 tgagatgtcg gggctggata actaccggat tttaaacgaa ttattaacga taaatttagc
     3601 acaacagcaa cgtttttttg ccgaagagca actcaaaggc aatttacctg cttcttattt
     3661 tgctcaaatt gccacacaag atttgatcgg caaaattacc gaaatgcaag cggcgttaag
     3721 ccactattta gccagcgaaa gccgtatttt gagtgttaat cgggaggttg agctggaacg
     3781 ccaaaccctc accctaaacg gcaatattgc caacctatat cagaatgaag tggtgctatg
     3841 gcgagtaggg catttacgag ataaagacaa gattcagact tggatctacc atttattatt
     3901 gtgtgcctat ttccctgaaa aaacggtgaa attctattac cgagagggtg agcaagttgg
     3961 tgtgttgagc tttgaaaccg tctcgcagat agaggcatta tcgcaattaa gcatttattt
     4021 gaatgcttat ttggacggat tatcgcaagc ccagttagtg atctacgacc gaattgagga
     4081 atatctcaaa ggtgatggca caaacagcga acaagttctt atcgatttag cagagaaaga
     4141 tgagcgagac ggcagctatc tccatcggct tctcgctcaa actaaagagc tggattctca
     4201 agcgattcac caacgtactc aaagctggtt tagcttaatg aatcagaaaa tagcgaaagg
     4261 cgattaacta caagcggtcg tttttggctg aaaatttgca aaatgagaag gcgaataata
     4321 attcgcctac ctataaaaat agaatttttt ccgtatttta ctgtcaattt attactacaa
     4381 tgctaatttc aaaaggagat gtatgaaatt atcttttctt acccaattcg ttgcagcggc
     4441 ttttgccctc aatgtgatga cttaccctgc atttgcggaa gttgcaaaaa attctgaaaa
     4501 aacgaccgct agtcaacccc aatcccaact cggttttgag ctgattaaac agagcattaa
     4561 caaaagcccg aatgataaag cgatttatca aggtattaaa cttgataatg gaatggaagt
     4621 gctactgatt tccgacagtc aagcgaataa atcgttgatg tctgccatta ttccgattgg
     4681 ctcaatggat gacccgattt ctcagcaagg tttagcccac tatcttgagc atatgatctt
     4741 gatgggctcg aaaaactatc cggaaaccaa cagcttagac ggttttttaa ccaaaaatgg
     4801 cggtatgaat aatgcctcga ctgcttctca ccgcacggcg tattatctac aagtcaataa
     4861 cgaagccttt agcgaagcgg taacacgact ggcggatacg ttagctttcc cgttattgct
     4921 ggaaaccaac gccaagaaag aggttaatgc ggtcaatgcg gagatggtgc gagctaaatc
     4981 gcacgatggg catttattgc acagtgttaa tttagccacc gcaaacccgg ctcatcctgc
     5041 taccaaattt gcggtaggca ataaagagac gttatctgac aagccggata gcaaattaca
     5101 aaccgagttg gagcaatttt ataaacagca ttattctgcg aatctgttta aagtggtgct
     5161 ttattccaat caatcgattg agcagatggc aaaattggca gccaacactt taggcaaaat
     5221 ggaaaacaaa catttcagca taccgcaaac cgctgagcca cgttatcgtg atgaggacaa
     5281 atcggtattt attcaatata agccggtgaa atccaacaaa atgttagtca ttggctttga
     5341 ttttccgaat gacgaaatgc agtttaagca caaaaccggt aaatatattg cttatatgtt
     5401 aaacaataat actgacggca cgctttcgga ttacttgatt aaacaggggt tgtctgacgg
     5461 cggtattgag gcggttagca gtgccaatat cgggcgggat cgaggctcgt ttgacatcta
     5521 tattgaactg acagacaaag gtttgagcga gcaagatcaa attatttcat tggttttcca
     5581 acaaattgag aagatcaaac aagaaggcat tcaagagagc tattttagcg aagtgaaaga
     5641 gagcctgaag caggatttcc aacatttgca agtggaaaaa aatatggatt tcgtggaatt
     5701 tattgccgat caaatgctcc atttcccgtt agaacacgtt atcgatcagc cttatctgac
     5761 cgaaacaatg gataacgagg caattaaagc aaaattggcg gcaatgaata ttgataatgc
     5821 ccgcattatg ttagtcagcg atactgccaa aaccgacaag aaaacccctt attttgaagc
     5881 aggttattcg gtggcaaaaa tcacgcaaga acaaaagcaa aaatggctgg atttcagtaa
     5941 aaatccacag ctcaatttgc ctgaattgaa cccgtatttc actaccgatt ttaccttaaa
     6001 caaggtcgaa accggcttaa ctaaaccgaa attgattaac gaccaagcgg ggaatttaat
     6061 ctatacaatg ccgagcgtgt attttaccaa tgaaccgaag gctaaaatcg gcgttggctt
     6121 taatattcgc ccgagaaaag atgacttaaa gagcctgacc gcagcgtcta ttttgggtta
     6181 tatgaacaat ttagcccaaa ccaagttaga ttttcagtct tcggtggcgg gtatgaattt
     6241 aagtttagct ccggcggcaa acggtttagc cttaagtgca gagggttata cccaaaatct
     6301 ggctaaatta atgctagaca gcgtaaaaca gttcagccgc tttgagttta acgaagacgt
     6361 gttggcacag gctaaacaac gtttgcacca agagctggat cgtaacgaaa aagaaaacag
     6421 ccttaatcaa gctaatgcag cattaagccg ttttgcctct tacccttatt ttgatgtgcc
     6481 gaaacagcgt gagatgattg atagcattac ccttgcggat attgagcaac tacgccagcg
     6541 aattttgcag cagagcagcg gctttaagtt gttatcggtg gggaatttgt cggctgagca
     6601 ggtttctgct ctggcagagg atttacaagc ggtcataaaa aaccaaaaat ctgcaaatgc
     6661 taccggacag tatgtggatg tgagtaccag tcagcgtaag attaatgtga ttaaaagcgt
     6721 gccgaatgaa gataatgcgt tgctaatggc atttatgccg aacggctata acgagctgga
     6781 aagccttact cgagccaaat tgctcagaag cattatttcc cgttggtatt ttgatgattt
     6841 gcgtactgat aagcaactag gctatgtggt ttatgcgaca gataatatca tcgggaaaac
     6901 cgccggtatc cagtttatgg tacaaagccc gaataccaca ccggcaggga ttttagagca
     6961 taacgaacgt ttctttacac aaagtttcga acgtttaaaa aatctatctg atgaggaatt
     7021 tggaaaatat cgtgatagcc tagtggaaaa actgcaatat aaaccggagt ctttagcgga
     7081 agagttttct gagttcggtt cagactttag cagaaataac ccgaaatttg accgcttgca
     7141 acaggtgatc gaattaacca aagcactcac caaagcagat attgtggcat tttaccaaaa
     7201 agctgtgatt gaaaagcaag gcttcgtgtt tgtcagccaa gctgtcggca ccaaaaccaa
     7261 agctgaagac gttgcgaaat ttgacggttt tgaacagatc gaaagcattg aaaagctaca
     7321 acgagagttt gagattaagg attattagag agttattaga gagagttagg ggcgaaaaat
     7381 tttcgcccct cattttttaa tccacaattt tcaccggata taagtaagta tatccgaacg
     7441 gactttgttt gtagccttca acacgtttgc tggttggggc aaagttaatt gagtgagcca
     7501 cgttaatcca cggagcttgc tctcttaaga taacctgtgc ctgctcgtat aacttagtac
     7561 gttctgcttt atcagatgag cctaatgctt tttctaataa cgcatctaac tcagggttag
     7621 taaagcgagc atagttactg ttgccgatat ttggcgtgcc gaatagtggc gataagaagt
     7681 tatccggatc gccgttatcg cccgaccaac cgtaagttcc ggcggttaat tcaccggctt
     7741 tggtacgctt gatgtaatca ccccattcat aggtgactaa ttttgctttt acgccgattt
     7801 tttcccagtc ggcttgaata atttcagaca tacggcgtgg gttcgggtta gaagcacgca
     7861 ccacaggctg cacccataat tcggtttcaa aaccgttcgg gaagcctgct tcagctaata
     7921 gttgcttcgc tttttccacg ttatattctg aatccgctaa gctgtcgtta tagccccaaa
     7981 tggttggcgg aagtgggttt ttcgccgcaa aaccgttacc ttggtaaact acatcaataa
     8041 tcgctttttt atccactgct aagtttaacg cttggcgaac tttcacgtta tcaaacggag
     8101 ctttttcggt attgaatgcc acataagcaa tgttcaaacc cggttgagaa agtaaattga
     8161 ttttcggatc atttttcatt ttctcaatgt cggttgcatt tgggaagtcc attaaatcac
     8221 attgaccgga ttgtaatttc gcataacggg ttgttgcatc cggcacgatc tcaaaaatca
     8281 aacggtcaat gtcaggctta cctttccaat actctttgtt tgctacataa cgactggctt
     8341 ggtctagcac ataaccgcta aacacaaacg gacctgtgcc gattggcgtg gtatctaccg
     8401 cttcaggtat gccggctttc atcattttat ccgcatattc cgccgaataa atggaggtga
     8461 aatccatacc aaggctggat aagaatgacg catcacgttt ggttaaggta atacgaacgg
     8521 tgctgtcgtc cactttttcc accgatttca ataatgtcgg gaatttcatt gcgttgaaat
     8581 acggataagt gccgttagag acggtgtgat acggatgatt tttatctaac tgacggttaa
     8641 atgaaaatac tacatcgtcc gcattcaatt cacggcttgg agtgaagtct ttgtttgaat
     8701 ggaatttcac gcctttgcgt aatttaaagg tgtaagttaa gccgtcttcg ctgatttccc
     8761 agctttctgc caatgccggt tcaatatcgg ttgtacccgg cacaaattcc accaagcggt
     8821 tgtaaatttg ttgcgagctg gcgttataag ataaaccgtc catcattaaa atcgggctaa
     8881 aaccggttgg cgaacggcta acgcagttta caaaggtttt ctcggtttgc ggttttgctg
     8941 ctgccgcagt aggtttattt tcgttgcttt tatcatcgca agccgctaga gctaataccg
     9001 ctaaaacgct tgcactaatt ttggttaacg ttggaaattt catattattc tccgcttaat
     9061 cttaggatta acttacgaag attacctttt ccacaatgag gagtaaagaa caaaaagcat
     9121 tatttttgca aatcaatcca tagaattaga taccgtcaac gtcattgcca cccgtgatcc
     9181 aagtaaattt gccgatacgc cacaaaaaca aactaaagat gcacttttgg taaaacaagc
     9241 gacgagtgtg gcggcggcat taaaagcgtt gccgaatgtg gatgtgcaag gaggggcccg
     9301 tgcgattgtg cagaagccgt ctattcgtgg gttgagcgat aatcgtattg tgcaggtgat
     9361 tgacggagtg cgtcagaatt ttgatttggc tcaccgtggt tcttattttg tgccgatgtc
     9421 attgattcaa gaaattgaag taatcaaagg tccaagcagc tcgttgtggg ggagcggtgc
     9481 cttaggtggg gtggtggcaa tgcgcacacc gaatgcgttg gatttattga aaaataatga
     9541 caaattcggg gcaaaaatcc gtcaaggtta tcaaacggcg aataatcttt ccgaaaccga
     9601 agcctcggtg tttgcagcaa atgatcggtt tgatgccctg ctgagtggtt tctacaataa
     9661 tgctgacaat ttgcgtagcg gtgaaggcaa ggaactgaat aacacgggct ataagcaagc
     9721 cggcggttta gtaaaattcg gctggcaaat taacgatact catcgtttgg aattatccca
     9781 tcgtcatagc caatttaaac aaactgcacc gggtaataac gaggtagaaa atcaatttac
     9841 cgataatgat gttagtgcac aaattagtgc ttggcatgcg gctaatcctg gaaatccaag
     9901 taatccagta tatcttgcga aaatgttagc cttttacggt caattacatt caagtcttgg
     9961 tagtgtgagt tatttaagtg atcaaaaaat taccgataaa agcacctcac ttaactacta
    10021 tttcaaccca agcaatcctt atttgagcac acaagtgaca ttatatagca atagtaccac
    10081 tgaaaacgaa catcgaaaaa taagtggtgt ggaagatcaa actaaactaa gcactcgtgg
    10141 tgtgaatatc cgtaatcatt ccgaattgtc ttggttctcc ttggtgtatg gcgtggatta
    10201 tatgttggat aaagtagcca ctcagcgtgg gagaaataat acggacgcac aataccgtgc
    10261 taatccttat gatgctaaat ctaaaactac aggtgcttat ttaattgcac atattccgct
    10321 gtttgatgaa aaagtggtct tttctccgag cgtgcgttac gaccgtttcg atacttcaag
    10381 tgaaacggtg aaatataaag acagccattg gtctcctgct gcaaaactta cttggaaagc
    10441 gacaaattgg ttggatttaa ccgcgaaata taacgaagct ttcagagccc cttctatgca
    10501 agaacgattt gtgagcgggt ctcattttgg taccacagtg agaggattac ctattaataa
    10561 tattttctta actaacccta atttacgccc ggaaacggca aaaaataagg aagtaacctt
    10621 caatgtacat tttgataatg tcatctcgca aggagataaa tttaactttg aagcgactta
    10681 tttccgtaat gatgtgaagg atttgattaa tcttcaagta ttcaaagcaa atcctacaag
    10741 cccaatggaa tttattccca ctcgttccca atatcaaaat attgcgaatg cacgtttgag
    10801 tggtattgaa ttacaaactc gttatcaaac tgaacgtttg gcagtttttg ctaactacgg
    10861 tagtacaaaa ggtaaagata aagacagcgg ggaagccttg tcgaacattg ctgcaagcaa
    10921 aatcggcttc ggggtcaatt atgccgtggt gcaagacaaa ttcacggtgg gggcaaacgt
    10981 aacccgttat caagcacaac atcgtgtgcc gaaaaaccac ggcgtgactt accaaggcta
    11041 tacgctgacc gatctacacg ccacttacgc cccattaaaa ggcgaatgga aaaacctgcg
    11101 tttagacgtg gcggttgaaa acctattcga taaaaaatac caaccggcat tcagcttaat
    11161 ggaaggcagc ggtcgtaatg tgaaactcag tgcggcttac agcttctaat gtctttaacg
    11221 gaagacggca caagcggtcg ttttccggtt attttttgca atattaaaag ggagttacta
    11281 tgaaatttta tccgtcgctg atcgcaagtg cggtcagttt tgccgttgtt ttgagtggac
    11341 aagcggcagt gaatgtgccg gatgccggct cgttacagcg tgaattagac caacgtcaag
    11401 ctcaacaaaa agtcaaaccg agtggcgaat ggtttaatca agcggcaaaa gcaccttata
    11461 ccgcaacgga cggtcaagaa ctgacatttc cattaactca ggtacaagtt tttgatcgaa
    11521 ccaaccaaga aatctctacc ccggaattag ccccgatttt ggcaaactat gtgggtaaag
    11581 cggtttcgct gaccgattta agcaacttgg caaatgagat caccgagttt tatcgtagcc
    11641 acaattatct ggtggcaaaa gccattttac cgccacagga aattgaacaa ggtaaggtga
    11701 aaatcctgtt gtttaaaggc aatgtcggtg aagtgcggtt acaaaatcag agtgctttaa
    11761 gcgataaatt tgttgcacgc cttgccaaca ccacagtgaa tggttcggaa tttatcctaa
    11821 aagacgaatt ggaaaaattt gccctcacga tgaatgatgt tcccggggtg aataccggct
    11881 tggagctttc tgccggtaag caagcaggcg aggcgaattt gctgattaag ctccaaaatg
    11941 ccgagcgttt ctcaggctat gtatcggcag ataaccaagg caataaaaat accggacgtt
    12001 accgtttagc cacagggata aaagccaata atctgatagg ttggggcgat gagttgaaag
    12061 tggatttgat gagttccaac aacgctaatt taaaaaatgc gcgtttggat tattcgagct
    12121 tgattgacgg ctacgctacc cgtttgggct taacggcgaa ttatctcgac tacgaattag
    12181 gcggtaactt taaagccctg caatcccaag ggcatactca caatcttggg gcttatgtgt
    12241 tgcacccgac aatccgcttg ccggattttc ggttaaacac caaagtcagc tttaatcatc
    12301 aacgtttaac cgacaaacaa caagcggtca atgtggcaca aaaacgccaa gtaaacagct
    12361 taacggtggg cattaacggg tcgtggaatt cgcttaaaga cggcacaagc tatttctctc
    12421 tgtctgcctt gttcggcaat ttagctaacc acaccaacga gcaagcgctg ccaaaatcct
    12481 ttgccttcaa ccttggcgtg aacgggcagt ttgcggataa aaccctcgac agctcacaaa
    12541 aaatgttact gggcggttta tacggcgtgc gtggtcatca agccggtgtc gcttcggtgg
    12601 atgaggggca tttagtgcaa accgaactca agcattattt accgctcttt tccaaaagcc
    12661 tgctgaccag ctcggtcttc tatgattatg gttggggaaa atactataaa cacagcggat
    12721 ttttggcgcg tggtgtaaac aatagcgtga aattacagag tatcggagca gggctttcgc
    12781 tctctgaagc aggcagttat gccattaacg ccagtgttgc caaaccgctg ggtaaaaaaa
    12841 tcgagaatgc agataaacac caattctggt tctccgccat caaaaccttc taaggagaca
    12901 aactatgttt aaacgtaatg caatttctct ctgcattttg accgcttgca gcggtttagc
    12961 ctatgccaat acagcagatt tgccgcaagg tgaaagggtg cgtgtcggga cggcaacgat
    13021 tgagcgcaca accgataaaa tgaccgtgaa tcaatccagc aataaagtac aaattgattg
    13081 gaacagcttt gatattggta agaataaaga agtggaattc aaacagccga cagagatggc
    13141 ggtggcatat aaccgtgtta ccggcggaaa cgccagtcaa attcaaggca agctgaccgc
    13201 aaacggcaaa gtgtatttag ccaacccgaa cggtgtactc atcaccaaag atgcggaagt
    13261 gaatgtgggc ggtttgttag tgaccacaaa agatttagaa aaaatcagcg aaaacggcac
    13321 aaaagataaa ttcagtcgca aattagcgaa agaaggcaaa attcttaacc aaggtaaaat
    13381 tacggccaaa ggcgagcggg cttttgtggt attggtgggc gacgatgtgg ctaatgaagg
    13441 ggaaattaac gccaaaaatt attaccacaa agaaacgaaa aaagtcaccg aatgttatga
    13501 cgattcttgg agcttggtaa aacaatgcta cgattttgat aaagaaatcg tgaaaagtac
    13561 cagtggagaa gtgcatttat cctccggaga aaatgtcacc tttactttaa gcgaccgcta
    13621 tattgatgtg gcgttagacg ataatacggt caatagcatt atcaaaaatg acggggcaat
    13681 catcgccaat ggtgacatta ccctcactgc caaagggcga aatcaagcct tagacagctt
    13741 aattatcaat aacggcgtat tgcaagccaa taaggtgcaa aataaaaacg gtacgatcat
    13801 tttatctgcc ggtgaaaccc aattacacga aaaaagccaa attaaaggcg agaaagtgag
    13861 ctttgccgaa gcttcttatt acggtccgac agaagacatt aaagtggttt ccaaaaccgg
    13921 ctcaaaagtg accgcacctg tgatcgattt caaaggtaaa accgtgaata ttcaagggga
    13981 atttgggcgt gaagacagca ccgatctcta taacgatgaa ctacaaatcc tcaaaaccac
    14041 ggtgaatatt gaagtcccga atacggaaga tattcgcatt gcagataccg acaacggagg
    14101 tagcggttcc tttatacaag cgggtgcttt agcctcatta cttgcaaata acggacgggt
    14161 taatgtacag ggcaaaggtt ttgatgtctc aggcaagttg aatgtgaatg cctttaaaca
    14221 aacggattcc gtgctgaaat ttatcaatca ggcgggcatc aatatccaca atgccgatat
    14281 tcacgcaaac gggcgtttat tctttgtgac aaacttgccg aatgacaccg atttccaatc
    14341 gaatatccgc attaccgaca gcaaaatcaa cttaggcaac ggctcaatgg gctttggtcg
    14401 cagtagcaaa cgttgggaaa tgcacgatgt ggttcgacgt gccgatacgg aactgcgtaa
    14461 aaaattcaat ctggatatga aaaacgtgga attaaaccaa gtggatgatt ttgtggtagc
    14521 gggcggtttc caacaagtta atttggataa cgtgaaagcc cgtggcaaaa ccaacttcta
    14581 tctggattcc ggcaactccc gcatgtttgt cggtgagcag atttatgaat atggcgtaac
    14641 cgatattgaa aaacgcaccc aattggcgaa tttagaccag cgtagaaaac gttggaattc
    14701 ggataactat gatgagaccg atctcaactg ggaatacaac caacgctttg acggctttgc
    14761 agcaaaacac aatcaccgcg cgccgattgc cgatacggaa atcaatattg ccaatagtga
    14821 tatcagctta gataacggtt ttgtgcattt aatggcagaa aaaatcaatc tggacgacag
    14881 caaaatcaat atcaccttca accaagacaa cagcttggat gaaagtaccc aaatcaaccg
    14941 cttaggacta aacggcaaag tgtcgatgaa aaacagccat attaacgtag tgggcgatga
    15001 aaaagacggt atctcaccgg ctcgccatta tgccacgatg ttcttagtgg gggagctgct
    15061 tggtgaaaag agttctattt tcctcaaatc ccaccaaggt gcagtattga gaaccgacag
    15121 caatgttaca attgccggta aaaacagcaa agacgacctc aaaattaccg ccattaccac
    15181 cggtaaaaaa atgggcaatg aaatcatcgg accggacggc ttgccaacct cggaagataa
    15241 cgatgccaac gccagcaaca ccgcctttgc catcggctat gaacctaacg ctacggctat
    15301 cgtcgaaaat gcaagcatta ccgcattagc accaaacggc ggcttggcat acaccggcac
    15361 gtctaaaacc caaattgacg cgaaggataa tgctaccttt accttctttg aaaaacctcg
    15421 agccggctat gacaaattgc ttggcgacat caaaggtaac cccgataaat taaccccacg
    15481 cgatttcgcc cgtttatacg acaacattaa aggcatcaaa gccagcagcc tatccgaaca
    15541 acagcttggc ctagcagaca ccgcgaagaa aaccagcgaa cacaccttag caaacagcct
    15601 agacgtagaa aatttggttt cagtgacagt gtgtgatgag cagaataagt gtgaggagcg
    15661 tatgttaggt aacaaaaata ccaatgctaa agttattgtc ggagatatca aatttgagta
    15721 gcgttgaaga aagtggtaga agctcagact tgatctgata aagcactata aaaagtgaga
    15781 gtttcccgtt tagaatatga gtgtcaaaat tcaatctaaa caaaaaggaa actctcatgt
    15841 tttattactt acaatcccct cattaaacac aagaccggtt tattaaattt agcagaagaa
    15901 ccgggcaata tctctcaagc ctgcaatgtg gtgggtatga gcctagatac attctattgc
    15961 tatcaacaag cggttatttt tggcaactaa tttgcaaaaa aacaataaaa tccgaccgct
    16021 tgtagagggt taatccgccc ttagtgaaat ttcaccgatg ccgaaatagc ggaggttgtc
    16081 gccctgtttt agaatgagtt cgccctgctc gttaatgcct tgcgaaatgc cgtggatttc
    16141 ttcggcttcg gtaatgagct taaccgcttt atggcggaaa aggtcgaaac tttgccagcg
    16201 ttcggcataa tgagcgaagc cgacaagcgg ataaattttg agattttttt gcaattcata
    16261 ggctaaacgg caaaccaatt cgttgcggtt gaaatgatat tgcgataaat ctgcccacgc
    16321 ttgcgtcaca atattttcat ccacttttgc cattcctagg ttcagcccaa tgccgataac
    16381 cacatctaaa ccattgcgat tggcttgggt ttccaacaaa atgcccccca tttttttgcc
    16441 ttggtaataa atgtcattcg gccatttaat ttgaatatct tttacatttt gaacagtaag
    16501 gctttccgca atgatcaagg ctaccactaa acttaagggc ggtaaatctg ccgcctgctc
    16561 catcgggtaa tgccataaaa tcgagaaata gagattttca ctctcgggcg aataccaagt
    16621 tcgacctcgt ctgccacgtc cggcagtttg tttttcagcg aggcaaaccg tgccgttttc
    16681 caaacgttgg tgatgagtga gcaaatattc attggttgaa tcaatttcat cgaacacaac
    16741 ggcttgcccg caaattaaag cattttggat ttgagcttgg ttaagtttca ttatttcccc
    16801 acttagcgag tgattttttc gctatcgacc tcaccgtttt taccgataaa acgcacttca
    16861 ggctgaattt ctaccccaaa tttttctttc actctctggc gaacggtttt ggctagagcc
    16921 aagacatctt gccctgttgc attttcttta ttaatcagca ctaaggcttg ctgggtatgt
    16981 accgccgctc cgccaatttg taagtctttc agcccggttt ggtcaattaa ccaacctgcg
    17041 gctaatttta ccgttccatc agcttgaggg tagtgaggaa tggctgggaa tttggcttgt
    17101 attggtgcaa attgttcgtt tgaaatcacc ggatttttaa aaaagctgcc ggcattgcca
    17161 aattcgtcag ggtttggcaa tttggaggag cgaaccgcac agacttcatc aaaaatctgt
    17221 attggcgtaa cggtttccgg attgagttgg gctaatgatc cgtaacttag caccggtttc
    17281 cacgctttgg ctaactttaa gcctaccgag ataatcgcaa attcatcacg gtattggtgt
    17341 ttaaacacac tttcacgata gccaaattcg cactcggatt tggatagctt aaagagttct
    17401 ccggaacgta aatttaacac ttccacgaaa tcacatacct gttcaaattc aaccccataa
    17461 gctcctatat tttgaatggg agcagagctg gcgacacccg gaattaacgc cagattttca
    17521 atgccggcaa tattgtgtgc caacgtccat ttaaccaatt cgtgccagtt ttccccacct
    17581 tgaacgtgca aatagtggaa atgttcgtct tcgtggtgcg taatcccttt gagtttattt
    17641 actaacacca caccgtcaaa atcctctaaa aacaaaacgt tagagccttg cccgagaatc
    17701 aagattggca gttgagcgtt aaaggctttt tgccattcgc tcaatagctg ttcgactgtg
    17761 gtaaattcaa taatttgggt cgcttttgca ggcaaatgga aagtgtgaaa aggggttaag
    17821 ctatgtttca tcatcaaatt agttagttaa gaagtggaaa taacaagcgg tgatattttt
    17881 acaaaatttt gcaattaaga ttttgggcag gcacaggatc tgcccctaca ttcagaatta
    17941 ccttgcccca tataccacaa tggttttgcc gtgagcagaa attaagcctt cgtcttccaa
    18001 catttttata atgcgtccaa cggtttcacg ggaacaaccg accatttgcc cgatttcttg
    18061 gcgggtaatt ttgatctgca taccttccgg gtgagtcatt gcatccggca ttttagctaa
    18121 gttgagcaag gtttgagcaa tgcgaccggc aacgtctaaa aaggcaagat taccgacttg
    18181 gcgtgaagtt tgacgtaaac ggcgagccat ttgtgccgat aaatacatta aaatatcggg
    18241 gtttaaatgg actagttgtt tgaatttttt ataggaaatt tccgcaattt cacaaggacc
    18301 tttagtgcgg acgtaagcgg tacgttgttg gactttttct tcaaataaac ttaattcacc
    18361 gacaaactca cctgtgccta agttagttaa gagcatctct ttggtttcat catctttgat
    18421 gtaaaccgac actgagccat tcacgatgta gaaaagtgat tctgcatctt caccggcgtg
    18481 aatgagtgta tatttcgccg gataacgatg gatatgacaa tgggttaaaa accactccac
    18541 cgcagggtcg tgaatagagg gcggaggagt tacattttcc agaggagctg ttgaaagtgt
    18601 catatcttgc atagtatttc ctataactct tatactaggt taaattttta gaattttaac
    18661 acgaaattaa aggttagcct aaaaaagatt ttttgtcttt aatatcaagg ctttcgtgta
    18721 attccgacca aacaatcacc actttatctg ccttaagttg tgtgagtaat cgttctcttt
    18781 tttctgccag agaaagttcc ctttccccgt agtctgtgcc ttctcgtaaa atgaaggaat
    18841 ctaaaatatt atgtaaggtt gattcttcga gttcttgcca aggtataatc ataattaatc
    18901 aaataaaggc ttaataaatg cccactgttt ttcaaaatgt tggtgttggt tatagctgaa
    18961 attagaacga accaagcgta aaaattgccc ttcgcagaaa ttcactaaat aaccggctaa
    19021 ggctctttcg tcttcaaagc tcttgcgttc acgcagttta ctaagttgca aaatattggc
    19081 aaattgtaac tccaagttat caaagaattt tgccactcga gctttgagca actcctcttc
    19141 aaacatcaag gcgtgtccgg tcaggatgcg ggtaacgccc gggtttttgc gagcaaattc
    19201 taaaatcaca tataaaattg ctcgcaccga attggcactg ctgtcttggc gtttattggc
    19261 gtgaatatag ctggtgaggg tctgctcgat tttctcgatt aacgcttcaa acatcttggt
    19321 tttacttggg aaatagcgat acaacgcccc ctccgacacc cccaccgcag cggctaaacg
    19381 ctcggtagtg atacgctgca ttccctcttc agaatttaac aagctgatta acacttccaa
    19441 cacttgttgg cggcgttcca ccactgattt tttcggcatt ttaattttag gttctgtcat
    19501 tgccaatcct attattgctt acctgaatgc ccaaaaccgc cttcgccacg gtcggtctgc
    19561 tcaaactctt gcacaatgtt aaaacttgcc tgaaccaccg gcacaaagac taactgagca
    19621 atacggtcgc ccacgttcac ggtaaaatca gcttggctac ggttccaaag cgacactttc
    19681 agctcgcctt ggtaatcgct atcgatcaag ccgactaagt tgcctagcac gatcccgttt
    19741 ttatgcccta aacctgatct tggtaagatc accgctgcca aattcggatc ggcgatataa
    19801 attgaaatcc ccgttgggat caacaccgtt tcacccgctt ttaccgtcag cggctcgttg
    19861 agcatcgccc gtagatccaa gcctgccgag ccttcggtcg catacgcagg taatggaaat
    19921 tcattgccga tacggctatc taaaa
//
LOCUS       DANLXS010000023        19668 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.023, whole genome shotgun sequence.
ACCESSION   DANLXS010000023 DANLXS010000000
VERSION     DANLXS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19668
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.023"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..287)
                     /locus_tag="PXD71_000662"
     CDS             complement(<1..287)
                     /locus_tag="PXD71_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL6290816.1"
                     /translation="MSIKKVTLASLILLGLTACSSGGSGSSSNNVAKPNTKPQTTQQQ
                     TDKAAAEKAAKDKAAKEKAEAERLAAEKAAKEKAEAERLAAEKAAKEKAEA"
     gene            490..1944
                     /locus_tag="PXD71_000663"
     CDS             490..1944
                     /locus_tag="PXD71_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDL6290817.1"
                     /translation="MSEISTLAPQLLWQWFDKACAIPHPSYHENELAEFIVNWAKSKN
                     FFVERDEVGNVLIRKPATKGMENRQTVALQAHLDMVPQANTGTVHDFTKDPIQPYIDG
                     EWVKAKGTTLGADNGIGLASCLAVLDSDDLAHPTIEVLLTMTEEAGMEGAIGLRPNWL
                     TADIMINTDTEENGEIYIGCAGGENVNVTLPIFLVPHQQDSAVTVTLKGLNGGHSGCD
                     IHTTRANAIKVFARILEQAHNEVEFQIAEIQGGSVRNAIPREAHATLVFSAKNQQKLT
                     ACLSQTAEQVKAELKIAEPNFQFLIEEAQLPEQAFDLASTARVIHFLNVMPNGVVRNS
                     DVVENVVETSLSIGVLNTEASEVKATILARSLIEGGKEEIRSKIRSLTALVGAEVEFS
                     GNYPGWEPNPNSKITPLTKAIYDEILGYESQIKVIHAGLECGLINKIYPNMDFVSIGP
                     TILNAHSPDEKVHIPAVEIYWKLLTKLLEQAPEK"
     gene            complement(2019..3308)
                     /locus_tag="PXD71_000664"
     CDS             complement(2019..3308)
                     /locus_tag="PXD71_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6290818.1"
                     /translation="MDIRQKIAHNKMTSFQWLVVGLTVLLNMLDGFDLMALAFTAKSI
                     REELQLDGAAIGSLMSAGLFGMAIGSLFLSPLADKFGRRPLLLIGTGLATLGMLLTYF
                     ATSAFEIGVWRVLTGLGVAAILTGTNVLVSEYSSQKWRGLAIAIYASGFGIGAMLGGM
                     SAVLLQGEYGWRAVFLVGSILTGAVFVLLFFVLPESVEFLISKHPVNAKARLNQIAVK
                     LGLGNGWELPEIEAKSKSKVAITRLFEPEYRRNTLLIWLAFIAISASFYFISSWTPAL
                     LEEAGMPKTQSQTVGMAISVGGTIGSLIFGFLVSRWAAKHILQAFSILAASAVVAFVF
                     AGNLGLAITLAILVGGLINGCITGLYTINPTLYAPDFRSTGVGTAIGVGRLGSIVSPT
                     LAGILLDAGWKKNELYLGAAVVLLIAAFTVHFLKQRK"
     gene            complement(3512..3847)
                     /locus_tag="PXD71_000665"
     CDS             complement(3512..3847)
                     /locus_tag="PXD71_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDL6290819.1"
                     /translation="MVSEQRQNPEEQKSEEQKINVEVLYAYPDKYFLKKLQVPQNATI
                     QTIILLSGVLEKYTEIDLRENKVGIFSRPAKLNETVEEGDRIEIYRPLIADPKEIRRK
                     RAAEQAEKK"
     gene            complement(3837..4271)
                     /locus_tag="PXD71_000666"
     CDS             complement(3837..4271)
                     /locus_tag="PXD71_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HDL6290820.1"
                     /translation="MPIVNQSALVPYSAEQMYQLVNQYEKYPEFLSGCVSTKTLSTGE
                     NELKAELHIQKLGISQTFSTHNKMTPPYKIEMALIEGPFRHLHGAWTFTPFDEQSCKI
                     ALQLNFEFSSPIVAMVFGKVFNELTLKMVNAFKQRAKEVYGV"
     gene            complement(4326..5381)
                     /gene="modC"
                     /locus_tag="PXD71_000667"
     CDS             complement(4326..5381)
                     /gene="modC"
                     /locus_tag="PXD71_000667"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDL6290821.1"
                     /translation="MLKLKLLQQLGNLTLNVNLDLPNKGITAIFGRSGAGKSSLINLI
                     AGLSTAQAGFIELNGQVLFDSQNGINLPPEKRNIGYVFQEHRLFPHYSVEKNLKYGYK
                     RSDSADFLQIVELLGIAPLLNRYPASLSGGEKQRVAIGRALLSSPNILLMDEPLSALD
                     LPRKQELLDYLDKLSARLEIPILYVSHSLEEVVRLADHLVLLENGKVTAFDTLSAVWH
                     SSAFSAWQPNHQRISLLELPVEETLPHYNMLGLSLNAQRLWVNQLPRHQIGEKLRVTI
                     ASKEVSISLQKPTQSSIRNVLKGNVRKIETHLDRVDLAIQVENQQIWSTISLWSFNEL
                     AIQPEQEVYLQIKSVSL"
     gene            complement(5368..6060)
                     /gene="modB"
                     /locus_tag="PXD71_000668"
     CDS             complement(5368..6060)
                     /gene="modB"
                     /locus_tag="PXD71_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDL6290822.1"
                     /translation="MLNFTPQELNAIVLSLKVASIAVVLALPLAVWVAWLLARKQFWG
                     KNLLNGIMHLPLVLPPVVIGYLLLISMAKRGVIGQYLWEWFHFSLSFSWKGAVLASMV
                     MAFPLMVRSIRLAFEAVDPKLEQAARTLGAIPLKVFFTLTLPLSFSGIIAGGVLGFAR
                     SLGEFGATITFVSNIPNQTQTIPAALFTFIETPDGEMAAARLCLVAILISLIALFCSE
                     WLAERQKRYAQT"
     gene            complement(6244..6999)
                     /gene="modA"
                     /locus_tag="PXD71_000669"
     CDS             complement(6244..6999)
                     /gene="modA"
                     /locus_tag="PXD71_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6290823.1"
                     /translation="MKALKKLAITMALTLSGTAFSQEITVFAAASMTNVLQDIQQDFE
                     KAYPQDKITFSFASSSVLAKQIEQDAPADIFISADQKWMDYVAEKQPEKVKHRRNLVR
                     NELVLIAPTESNIEPKAIQAVDFAKELANSYLSVGDPNHVPAGKYAKKALSHYQLWES
                     VEPRLARAKNVRDALSFVERGESPLGIVYSTDAKVSNKVKVVAVFPPESYGEIVYPAA
                     AVSEKASAATFLDFLKTPKAKAKFEAAGFYPVN"
     gene            complement(7020..7781)
                     /locus_tag="PXD71_000670"
     CDS             complement(7020..7781)
                     /locus_tag="PXD71_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6290824.1"
                     /translation="MNLIGFYTLAYKECRRILRIWRQTLVPPVITTTLYFLIFGTLIG
                     GRIGEMNGVGYTQFIAPGLIMMTAITASYVNTASSFFLSKFTRDFEEMLISPLSTHNI
                     IWGYVTGSILRAGLAATLVMCVAMVFVSFEIYSVAIIVLTLLLTIIAFALGGVINAVF
                     ARSFDDVGLIPTFVLTPLTYLGGVFYSISLLPEFWQTVSKFNPIVYMINGLRYGFLGI
                     SDVPLGYTFAVLTSLCIGLYIIAYSLIQRGVGLRS"
     gene            complement(7783..8691)
                     /locus_tag="PXD71_000671"
     CDS             complement(7783..8691)
                     /locus_tag="PXD71_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6290825.1"
                     /translation="MKALELIDLVKTYPTGVKAVKGINLVVEQGDFYALLGHNGAGKS
                     TTIGIISSLVNKTSGSVKVFGYDLDTQKVELKHQIGLVPQEFNFNQFEKVIDVLVQQA
                     GFYGIPRSEALVRAEKWLKLLDLWEKRTHLTRELSGGMKRRVMIARALMHNPKLLILD
                     EPTAGVDIELRRSLWDFLRDLNNQGTTIILTTHYLEEAETLCRHIGIIQHGELIENTS
                     MKALLAKLESEVFVLDLQNSEQNQPLVIQNYPHKWLDENTLEVNVKRQQGLTNLFSQI
                     SAQGAEVLSMRNKSNRLEELFINMAQ"
     gene            complement(8697..10754)
                     /gene="dnaX"
                     /locus_tag="PXD71_000672"
     CDS             complement(8697..10754)
                     /gene="dnaX"
                     /locus_tag="PXD71_000672"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL6290826.1"
                     /translation="MSYQVLARKWRPQRFSEVVGQQHVLSALENSLREDRLHHAYLFS
                     GTRGVGKTSIARLFAKGLNCVNGITAEPCGECANCKAIEEGHFIDLIEIDAASRTKVE
                     DTRELLDNVQYKPTVGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFLLATTDPQ
                     KLPITILSRCMQFHLRALEQSQIARHLEFILNAENIPFEPLALEKLAKAAQGSIRDSL
                     SLTDQAIAMSNANITLAIVSQMLGLIDDNQPLELVQATAQADGEKAMAIIEQIAAKGV
                     DWQQLLSDVAEVLHKIAMLQLVKTPTTEETLVHFLAKQIPPEDVQFFYQLILNSKKEL
                     PFAPEQRAGVEMAILRALAFHPKQAEKIATVAVEKVTQPNHSVSAVTTAKPQNQISQL
                     RENFEQNQQKTVTVANTTTAVESIAQPPQAVDTAISPSAPATLSPAMAALEARKKLQA
                     QQANLAQTEKKKPELKAPQVIAKKSANQTASLQERFISLAENQHKAVLQVSKKTESAP
                     KSVEDEDYRWTWLNPELENQADSAKPSDIKQAILQERTPELVAKTIHLSCQQDKWCDI
                     ANSLNLGGFSRQIALNSYLAEQNEQHLALVLKPNMAHLDNPESRKNLEAALAEKGYSY
                     ELTIGDNSEQQTPLEIRRAIFKQLTEAAKSALLNDEKLMLLRNAFDAQIDESTIRAVA
                     ETE"
     gene            complement(10845..11384)
                     /gene="apt"
                     /locus_tag="PXD71_000673"
     CDS             complement(10845..11384)
                     /gene="apt"
                     /locus_tag="PXD71_000673"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL6290827.1"
                     /translation="MNQLDLIKSSIKSIPDYPKAGIIFRDITSLLEVPTAFQATIDAI
                     VAEFKDKGITKIVGTESRGFIFGAPVALALGVPFVLVRKPKKLPREVISQSYTLEYGE
                     DTLEIHKDAVSANDNVLVIDDLLATGGTIDATAKLIRRLGGNVEHAAFVIWLPELGGK
                     ERLEKEGVKSFSLVSFEGH"
     gene            complement(11512..12462)
                     /gene="lpxM"
                     /locus_tag="PXD71_000674"
                     /gene_synonym="msbB"
     CDS             complement(11512..12462)
                     /gene="lpxM"
                     /locus_tag="PXD71_000674"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249087.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDL6290828.1"
                     /translation="MTRITAQDGYTHKFLWSFLHPKYWGVWLGVLGLVILAYVPVRIR
                     DKLSAFVGKMAYRYLKQKNKKGYHRAKVNLRYCFPDWSEKKREEVVEKMFITVAQTML
                     AIGETAIRPASYLQKRCEFTGFENVVKAKESGKNVIMLVPHTWSIDMAGVAMFSLGYP
                     MTSMYNPHRNALVDWLWNITRERLGGRMHTRQNGIKAFLADVRKGDVGYFLPDEDFGE
                     ELSVYADFFATEKATLPGLNKMARVANAEVIPMFSIYNAEKAKYEMEILPPLEFSGDP
                     IQSAREMNRVIEYFVEKNPEQYVWILRLLKTRRNREDIYL"
     gene            complement(12903..13586)
                     /locus_tag="PXD71_000675"
     CDS             complement(12903..13586)
                     /locus_tag="PXD71_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDL6290829.1"
                     /translation="MEREQQIPVTNIDVAPKQSSVWKTIFADGVWKNNGALVQLLGLC
                     PLLAVSNSVTNALGLGLATMFVLICTNITVSLFRKITPHDIRIPIYVMVIATVVTAVQ
                     LLMNAFAFPVYQSLGIFIPLIVTNCIVIGRAEAYASKNEVKFSAFDGFSMGLGMTFSL
                     VVLGALREIIGNGTLFSGLDLLLGDWAKALRIDVLHIDSGLLLAILPPGAFIGLGVIL
                     AVKNIIDKR"
     gene            complement(13595..14227)
                     /gene="rsxG"
                     /locus_tag="PXD71_000676"
     CDS             complement(13595..14227)
                     /gene="rsxG"
                     /locus_tag="PXD71_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDL6290830.1"
                     /translation="MNTSKITLNYALILGFIALLCTAVSTGVYLLTKGKIDDVTAMQQ
                     RKLLEEVVPKSHFDNDVLATCKLVQLENAPYLNRIYVAKKSENLTAYLVQGTAPDGYS
                     GDIVLLMGIEPNGNILGVRTLEHKETPGLGDKIETRVSDWILSFTNQLFSLENEADWN
                     VKKDGGKFDQFTGATITPRAVVNHIRQSAKWVVTELAQNPTMFNQFKACK"
     gene            complement(14227..15282)
                     /gene="rsxD"
                     /locus_tag="PXD71_000677"
     CDS             complement(14227..15282)
                     /gene="rsxD"
                     /locus_tag="PXD71_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDL6290831.1"
                     /translation="MFKMVSSPHTHSSNLTANFMLWVIAMMLPAFGVQLYYFGYGVLI
                     QTLLAVSLAIVIEIGIAKLRKKTTAFYLADLSGILTALILAMSIPPYAPYWLVLIGVI
                     VALLLAKHSYGGLGQNLFNPAMVGYALLLVSFPVQMTAWIVPIELLSEPPTFRDAISL
                     IFYGVTSDGFSIHQLVNSVDGIAQATPLDSMKSSLAKSGFDAVMNTPIFNGLFATGWL
                     QLNLAFLFGGLVLIYKRIIHWQIPVAMLATFAFFSGFTELFSQQSHLHFVSHFFSGAM
                     MFGAFFIATDPVTASITPRGKLIFGGLVGLLVYLIRYYGNYPDAIAFSVLLANICVPL
                     IDHYTQPRLYGTKLDNR"
     gene            complement(15328..15963)
                     /locus_tag="PXD71_000678"
     CDS             complement(15328..15963)
                     /locus_tag="PXD71_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDL6290832.1"
                     /translation="MFSELIPALPWSFLFIQDLIGTIVFAISGAIAARQHKMDIFGMF
                     ILAFVTGVGGGTLRDMMIGSTPVFWMKQPIYVLMITLAVVITAIFRNQISRKSWQTGL
                     LVFDAIGLGVFTVIGVQKGLDFGLHPLICVALGGVTGCFGGLIRDILRNEVPIILQKE
                     VYVTASLIGGVIFVAANSVGLESDWVYLATVFTVIIIRLLAIRFNLNLPRL"
     gene            complement(15956..18250)
                     /gene="rsxC"
                     /locus_tag="PXD71_000679"
     CDS             complement(15956..18250)
                     /gene="rsxC"
                     /locus_tag="PXD71_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDL6290833.1"
                     /translation="MSNPNLVIERIRQNKETGKLWDFPGGIHPMENKSQSNSKPIRTL
                     NLPKFFYVPLVQHSGWAGELLVKVGDVVLKGQPLTKGDHFRQLPVHSPTSGRVIGIEP
                     YVSAHASGLPEITVIIETDGKDKWVERQPIEDFLSLTSDQIIQKVYQYGIAGLGGAVF
                     PTATKLSLADKRCKLLVINGAECEPYITCDDRLMQDFTADLIEGIRILRYVLRPEEVV
                     IAIEDNKPHAIQALETALKGSNDMFVRAIPTKYPSGASDQLIQVLTGLEIPAGKRTIE
                     MGIVMHNVGTAFAVKRAIIDDEPLIERVVTLTGDKIRNKGNVWARLGTPIQHLLEQVD
                     YQADKRFPVFLGGPMMGFILPALQAPITKTANCLIAPDHFEYAPPEPERSCIRCSSCS
                     DACPVGLLPQQLYWFARAEDHDKSKEYHLDACIECGVCAYVCPSYIPLIQYFRQEKAK
                     IEEIEEKARKAEEAKIRFEAREARLQKERDARTARISQAAEKRREEVANAGGVDPVKA
                     ALERLKAKKAVETVQEIPAEVQVKPNGEPDNSDLMAQRRARRLAKQAEQAVEKSENVT
                     NALIGANNDLPLQSMNETASDKSVGANNYSPEAESEDPRKAAVAAALARAKAKKQAQQ
                     AVEKSENITNAVIGVNNDLPLQSMNETVSDKSVGANNHSPETESEDPRKAAVAAAIAR
                     AKAKKQAQQAVEKPEDIANAVIGVNNDLPLQSMNETTSDKSVGANNHSPKTESEDPRK
                     AAVAAAIARAKAKKAAQQEKSNNV"
     gene            complement(18237..18854)
                     /gene="rsxB"
                     /locus_tag="PXD71_000680"
     CDS             complement(18237..18854)
                     /gene="rsxB"
                     /locus_tag="PXD71_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDL6290834.1"
                     /translation="MLDLPIIAYILIAIAVIALIFGAVLGYSSIKLKVESDPIVDQID
                     ALLPQSQCGQCGYPGCKPYAEAIANGDVITKCVPGGQPLVVNLAELLGVEVPPMEGMA
                     EPEMKVAFVIEDLCIGCTKCIQACPVDAIIGTNKAMHTIIPDLCTGCELCVAPCPTNC
                     IEMRPIKPTTQSWNWKFDQDLIIPIVNTTELQRKLVTGGGKNEQS"
     gene            complement(18854..19435)
                     /gene="rsxA"
                     /locus_tag="PXD71_000681"
     CDS             complement(18854..19435)
                     /gene="rsxA"
                     /locus_tag="PXD71_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDL6290835.1"
                     /translation="MVDYILLVISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGLA
                     TTFVLTVASLSAYLMETYLLEPLNAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRL
                     LGIYLPLITTNCAVLGVALLNVNLANNLVESVLYGFGAAAGFSLVLVLFAALRERLAA
                     ADVPRPFQGASIALITAGLMSLAFMGFTGLVKL"
BASE COUNT         5578 a         4482 c         4119 g         5489 t
ORIGIN      
        1 tccgcttcgg ctttttcttt cgctgctttt tccgccgcta aacgttctgc ttccgctttc
       61 tctttagctg ctttttctgc tgctaaacgt tctgcttctg ctttctcttt cgctgccttg
      121 tcttttgctg ctttttctgc tgccgcttta tcggtttgtt gctgagtagt ttgcggttta
      181 gtattcggtt ttgccacatt gtttgagcta ctgcccgaac caccactact acacgccgtt
      241 aaacccaata aaatcaaact ggctaaagtt acttttttga tagacataaa atgttccttt
      301 tgtaagataa acaaaaatag atgtgtataa aacacacgca tattgtacaa aaaaaaaaca
      361 actcgtcatc aagtactttt taagattttt ttgttaaatt tctcctaaat attctacaat
      421 gtcagaggtc tatatcagct tgaatacaag cggttatttt ttcttaaaat tttacaaaag
      481 gggcttatta tgtctgaaat ctcaacgctc gcaccgcagc tactttggca gtggtttgat
      541 aaagcctgtg ctattccgca cccgtcttac cacgaaaatg aactggctga atttattgtg
      601 aattgggcaa aatcgaaaaa tttctttgtt gaacgtgatg aagtgggtaa tgtgttaatc
      661 cgtaaaccgg caactaaagg tatggaaaac cgccaaactg tcgctctgca agcccatctt
      721 gatatggtgc cacaagccaa taccggcacg gttcacgatt tcactaaaga tccgattcag
      781 ccttatattg acggcgaatg ggtaaaagcc aaaggcacaa cattgggggc agataacggc
      841 attggattgg cttcgtgctt ggcggtgttg gatagcgatg atttagccca cccaaccatt
      901 gaagtgttgc tcacaatgac cgaagaagcc ggtatggaag gggcgattgg tttacgcccg
      961 aactggctaa ccgccgatat tatgattaac accgacaccg aagaaaacgg ggaaatttac
     1021 atcggctgtg caggtgggga aaatgtgaat gtgacactgc caattttctt ggtgcctcat
     1081 cagcaagata gtgcagtaac cgttacgctt aaaggcctaa acggcgggca ttcgggctgt
     1141 gatattcaca ccacccgagc gaatgcgatt aaagtgttcg cccgcatttt ggaacaagcc
     1201 cacaacgaag tggaattcca aattgcagaa attcaaggcg gttcggtgcg gaatgccatt
     1261 ccacgagaag cccacgcaac cttggttttc tctgcaaaaa atcagcaaaa attaaccgct
     1321 tgtttaagcc aaaccgccga acaagtaaaa gcagaactca aaattgccga gccaaacttc
     1381 caatttttaa ttgaagaagc tcaactgcct gagcaagcct ttgaccttgc cagcactgct
     1441 cgagtaatcc atttcttaaa tgtgatgcca aatggtgtgg tgcgtaatag cgatgtggtg
     1501 gaaaatgtgg tggaaacctc actcagtatt ggtgtgctta acaccgaagc gagtgaggtg
     1561 aaagcgacca ttcttgcccg ctcattaatt gagggcggaa aagaggaaat tcgcagtaaa
     1621 atccgctcat taaccgcatt agtgggagca gaagtggaat tttcgggcaa ctacccgggc
     1681 tgggagccaa acccaaattc caaaatcacg ccattaacca aagcgattta tgatgaaatt
     1741 ttaggttatg aatctcaaat taaagtgatt cacgcagggc tagaatgtgg cttaatcaac
     1801 aaaatctacc cgaatatgga ttttgtctcg attggaccaa ctattctcaa cgcccattct
     1861 ccggacgaaa aagtgcatat tccggcagtg gagatttatt ggaaactcct aaccaaacta
     1921 cttgaacaag cacctgagaa ataaatttaa tcaaaaaaat tatataatct tgccccgtaa
     1981 ttaattgaat aaaacctcaa ttaattacgg ggtagctctt attttctctg ttttaaaaaa
     2041 tgtaccgtga aagcagcaat taataagacg actgctgcac ctaaataaag ctcgttcttt
     2101 ttccagccgg cgtctaataa aatacctgca agagttgggg aaacaattga gcctaaacgc
     2161 cctacaccga tggcggtgcc tacgcccgtg ctgcggaaat ccggtgcgta tagggttggg
     2221 ttaatggtat aaagcccggt aatgcagccg ttaattaatc cgccaaccaa aatggctagt
     2281 gttatcgcaa gccctaaatt gccggcaaac acaaaggcaa ccacagcaga tgcagccaga
     2341 atactgaatg cctgcaaaat atgttttgcc gcccagcgac tgactaaaaa gccgaaaatt
     2401 aacgagccga ttgtaccacc aactgaaatt gccattccca cagtttggct ttgggtttta
     2461 ggcattccgg cttcttctaa tagtgccgga gtccaagagc tgataaagta aaatgaggct
     2521 gaaattgcaa taaaggcaag ccaaattagc aacgtattgc ggcgatattc aggctcgaat
     2581 aaacgggtaa ttgccacttt gcttttactt tttgcctcaa tttcaggaag ttcccagccg
     2641 tttcctaagc ctaatttaac ggcaatttga tttaaacgag ctttagcatt caccgggtgt
     2701 tttgaaatta aaaattccac cgattcgggc aatacaaaga ataataaaac gaataccgca
     2761 ccggttaaaa tcgacccaac gaggaaaacc gcacgccagc catattctcc ttgcaataac
     2821 accgcagaca ttccgcccag cattgcacca atcccaaagc ctgaggcata aattgcaatc
     2881 gctaagccac gccatttttg gctggagtat tcactcacta gcacattagt gccggttaaa
     2941 atggcggcta cgcctaaacc ggttaaaaca cgccatacac caatttcaaa cgcagaagta
     3001 gcgaaataag ttaggagcat tcctaacgtg gctaagcctg tgccgattaa aagcaacggg
     3061 cggcgaccaa atttatcagc aagtggtgaa agaaagagtg agcctatcgc cataccgaat
     3121 aaaccggcac tcattaagct gccgatggct gcaccgtcta actgcaattc ttctcggatg
     3181 cttttggcgg taaaggcgag tgccattaaa tcaaagccgt ccagcatatt aagtaacacc
     3241 gtaagcccaa ccactaacca ttggaatgaa gtcattttgt tatgagcaat tttttgacga
     3301 atatccataa gctccctctg catcaaaatt gataaaattt tatttagagt gaactataga
     3361 aagttgatta aatataatca aactcaaaat ttacataaaa tttatgaaag attttcatat
     3421 ttaatcaaag gagggaattt atagcaggaa tggcgaggga gaaaatagat agggcgaata
     3481 attattcacc cctagattga ataggagaaa gctatttctt ctccgcttgc tcagccgctc
     3541 ttttgcgtct gatttctttc ggatcagcaa ttaacgggcg atagatctca attctgtcgc
     3601 cttcttccac cgtttcattc agttttgccg ggcggctaaa aataccgact ttgttttcac
     3661 gcaagtcaat ttcggtatat ttttccaaca cgccggagag caaaataata gtttgaatgg
     3721 tcgcgttttg cggtacttgc agttttttca gaaaatattt atcagggtaa gcataaagca
     3781 cctcaacatt aattttctgt tcttcacttt tttgttcttc tggattttga cgttgttcag
     3841 acaccataca cctcttttgc acgttgtttg aaagcattta ccatttttaa agttaattcg
     3901 ttgaacacct tgccgaacac cattgctaca attgggctgg aaaattcaaa atttaattgc
     3961 agggcaattt tgcaactttg ctcatcaaag ggggtgaatg tccaagctcc gtgtaaatgg
     4021 cggaaaggcc cttcaattaa tgccatctcg attttatagg gcggcgtcat tttattgtgg
     4081 gtactgaaag tttggctaat gcctaatttc tgaatgtgta actctgcctt taattcattt
     4141 tcgccggtgc taagggtttt ggtactcacg cagccggata aaaattcggg gtatttttca
     4201 tactgattaa caagttgata catttgctcg gcactataag gcacgagggc agattgatta
     4261 acgattggca taatgtctct tttgggaatt aacaagcggt catattttag cgtaaatttg
     4321 caagcctata aagaaacgct tttgatttga agatagacct cttgctccgg ttgaatcgct
     4381 aattcattaa aagaccaaag gctgatcgta ctccaaattt gctgattttc gacttgaatt
     4441 gccagatcaa cccgatctaa atgcgtttct attttccgaa cattgccttt taacacatta
     4501 cgaattgagc tttgagtcgg tttttgtaag ctgattgaaa cctctttgct ggcaatagtt
     4561 acccttagct tctcgcctat ttgatggcgg gggagttggt tcacccataa tcgctgtgcg
     4621 ttcaagctca agccaagcat attatagtgg ggaagtgttt cttccaccgg taattcaagc
     4681 aggctaattc gctgatgatt tggctgccaa gcggaaaatg ccgagctgtg ccaaaccgct
     4741 gaaagggtat caaatgcggt tactttgccg ttttccaaca gcacaagatg atctgccaat
     4801 cgaactactt cctctaaact gtggctgaca tacagaatcg gtatttcaag ccgagcggat
     4861 aatttatcta aatagtccag tagctcttgc ttacgaggga gatcgagggc agataatggt
     4921 tcgtccatca gcaaaatatt cggtgagctt aataacgccc gcccaatcgc cacacgttgt
     4981 ttttcgccac cggaaaggct tgccggatag cgattaagca aaggggcaat gcctaacagt
     5041 tccacaattt gcaaaaaatc cgcagaatcc gaccgcttgt agccatattt taagtttttt
     5101 tccaccgaat aatgagggaa aaggcgatgc tcttgaaaca catagccaat gttgcgtttt
     5161 tcaggtggta aattgatgcc gttttggctg tcaaacagca cctgaccgtt taattcgata
     5221 aagccggctt gagcggttga aagccccgca attaaattaa tcaggctgga tttaccggca
     5281 ccggaacgcc cgaaaatagc ggtgattcct ttattcggca aatcgagatt gacattgagc
     5341 gtcagattgc cgagttgctg aagaagttta agtttgagca tagcgttttt gcctttctgc
     5401 cagccattcc gaacagaaca gggcaatgag tgaaatcaga atcgccacca agcacaaacg
     5461 ggcggcagcc atttcgccat cgggggtttc aataaaggtg aaaagggcgg caggaatggt
     5521 ttgggtttgg ttgggaatgt tggagacaaa ggtgatggtt gccccaaact cccccaaaga
     5581 gcgggcaaaa cccagcacgc caccggcaat aatgcctgaa aaagagagcg gtaaagtgag
     5641 cgtgaaaaac acttttaacg gtattgctcc aagcgttctg gcggcttgct ccaatttagg
     5701 atcaactgcc tcaaaggcta aacggataga acgcaccatt agcggaaacg ccatcaccat
     5761 tgaggcaagc actgctcctt tccacgaaaa actcaagctg aaatgaaacc attcccacaa
     5821 atattgcccg attacccctc gtttcgccat cgaaataagc aataaatagc caatgacaac
     5881 cggcggtaac accagaggga gatgcataat accattgagc agattttttc cccaaaactg
     5941 tttgcgggca agcagccacg ccacccacac ggcaagcggt aatgcaagca ctacagcaat
     6001 ggaggcaact tttaggctta gaacaatcgc attgagttct tgtggggtga aattaagcat
     6061 aatctaaaac tatttggtta taagcttacc ctcgcccctt gtgggagagg gatagatttt
     6121 tcttcgttca gaagaaaaat cagggagagg gggaatattt taaacgctat ttgccctctc
     6181 tctgaaaaat atgtctgaac gacatatttt tctgtctctc tcccacaagg ggagagagta
     6241 aatttaattt acaggataaa acccagctgc ctcaaatttc gcttttgctt tcggggtttt
     6301 gagaaaatct aaaaatgtgg cggcagaggc tttttcactg actgccgcag cagggtaaac
     6361 aatttcgccg tagctttccg gtgggaaaac ggcaaccact ttgactttat tcgacacttt
     6421 agcatcggtg gaatagacaa tcccaagtgg ggattcgcct cgctcgacaa aagagagggc
     6481 atctcgtaca ttttttgccc tcgctaaacg aggttcaaca ctttcccata attggtaatg
     6541 actaagggct tttttggcat atttgccggc aggaacgtgg tttgggtctc ccaccgataa
     6601 atagctgttt gctaattctt tggcaaaatc gaccgcttgt atcgcttttg gctcaatgtt
     6661 actttcggta ggtgcaatta atactaactc gtttctgact aaattgcggc gatgtttaac
     6721 tttctcaggt tgtttttcgg caacataatc catccacttt tggtcggcgg aaataaaaat
     6781 atctgccggt gcatcttgct caatctgctt tgctaatacc gatgatgaag cgaaggagaa
     6841 cgtgatttta tcctgtggat aggctttttc aaaatcctgc tgaatatctt gtaacacatt
     6901 tgtcattgaa gcggcagcaa acacggtaat ctcttgtgaa aaggctgtgc ctgataacgt
     6961 taatgccatt gtaattgcta gtttttttaa tgctttcatt ttcataacct cacaattagt
     7021 taagaacgca gcccaacccc tcgttgaatt aaggaatagg caataatata caagccgatg
     7081 caaagtgaag taagcactgc aaaagtataa cctaacggca catcgctgat accgagaaag
     7141 ccatatctta agccgttaat catataaacg atcgggttga atttagacac cgtttgccaa
     7201 aattcaggca gtaatgaaat agagtaaaat acgccgccta agtaagttaa gggagtaagc
     7261 acaaaggttg gaatcagccc cacatcatca aaggagcggg cgaacaccgc attaattacg
     7321 ccacctaacg caaaggcaat gattgtcagc aacagggtta agacgataat cgcaaccgag
     7381 tagatttcaa acgagacaaa caccattgcc acgcacatta ctaaggttgc ggctaaacct
     7441 gcacgcaaaa tactgccggt gacataaccc caaataatat tatgggtaga gagtggcgaa
     7501 atcagcatct cttcaaaatc tcgggtaaat ttactcaaga aaaatgaaga ggcggtattc
     7561 acataagagg cggtgatggc ggtcatcata attaagccgg gtgcaataaa ttgggtgtaa
     7621 cccacgccat tcatttcccc gattcgccca ccaatcagcg tgccgaagat taaaaaatag
     7681 agcgtggtag taatcaccgg tggcactaaa gtttgtcgcc aaatccgcaa aatgcggcgg
     7741 cactctttgt aagctaaggt ataaaatccg attaaattca ttttactgag ccatattgat
     7801 aaatagttct tccaagcggt ttgatttgtt tcgcatactt agcacctcag ccccttgggc
     7861 ggaaatttgc gaaaacagat tggttagccc ttgctggcgt tttacattca cttcaagggt
     7921 gttttcatca agccatttat gcgggtaatt ttgaatcaca agcggttgat tttgctcaga
     7981 attttgcaaa tctaacacaa atacctcact ttccaatttg gcaagcaagg ctttcattga
     8041 ggtgttttca attaattcgc cgtgttgaat aatgccgatg tgacggcata gggtttccgc
     8101 ctcttctaaa tagtgggtag tgagaataat agttgtgcct tgattgttta gatcccgcaa
     8161 gaaatcccac aatgagcggc ggagttcaat atccacccct gcggtaggtt catctaaaat
     8221 cagtagtttt ggattgtgca ttaaggctcg ggcaatcatc acccgacgtt tcattccacc
     8281 agaaagctca cgggtgaggt gggtgcgttt ttcccatagg tcgagcaatt tcagccactt
     8341 ttcggcacgt actaaggctt cactgcgagg aatgccgtaa aacccggctt gttgcactag
     8401 cacatcaatc actttttcaa actgattgaa attaaactct tgtggcacta agccgatttg
     8461 atgctttaat tcgacttttt gggtgtctaa atcatagcca aacactttca cgctgccgct
     8521 ggttttattt accagtgagc tgataatgcc aatagtagta gatttacctg ccccgttatg
     8581 cccaagtagg gcatagaaat caccttgctc gaccacaaga ttaatgcctt taacggcttt
     8641 tacacctgtt gggtaggttt ttaccaaatc aattaattct aatgctttca tttgttttac
     8701 tcagtttccg ccaccgctcg aatggtcgat tcatcaattt gtgcatcaaa agcgttgcga
     8761 agtaacatta atttttcatc gttaagcaga gctgattttg ctgcctcagt taattgtttg
     8821 aaaatggctc gccgaatttc aagcggagtt tgctgttcgc tattgtcacc aatggtcaat
     8881 tcgtatgaat agcctttttc ggcaagggca gcttctaaat ttttacgtga ttccggatta
     8941 tctaagtgag ccatattcgg ctttaacacc aatgcgaggt gctgttcatt ttgttccgct
     9001 aaatagctat ttaaggcgat ctgacgggaa aatccgccta aattgagtga gttggcgatg
     9061 tcacaccatt tatcctgctg acaagataaa tgaatggttt ttgccactaa ttcaggggtg
     9121 cgttcttgca aaatcgcttg tttaatatca gaaggtttgg cactgtctgc ttgattttct
     9181 aattcaggat tgagccacgt ccagcgataa tcttcatctt ccacactttt aggggcggat
     9241 tcggttttct ttgaaacttg cagtacggct ttgtgctgat tttccgccaa gctgataaac
     9301 cgctcttgca atgaagcggt ctgatttgcc gatttttttg caataacttg aggggctttt
     9361 agctcaggct tttttttttc ggtttgagct aaatttgcct gctgtgcttg tagctttttg
     9421 cgggcttcga gggctgccat tgccggtgaa agagtcgcag gagcagacgg cgaaatagcg
     9481 gtatctactg cctgtggcgg ttgagcaatg ctttcaaccg ctgtggtagt attggcaaca
     9541 gtaaccgttt tttgctgatt ttgctcaaaa ttttctctta gctggctaat ttgattttgt
     9601 ggttttgctg tcgtaaccgc tgaaaccgaa tggtttggtt gtgtaacctt ttccactgca
     9661 actgttgcta ttttttctgc ctgtttaggg tggaaagcca acgcacgcaa aatcgccatt
     9721 tcaacaccgg cacgttgctc cggtgcaaaa ggcaactctt ttttactgtt gaggattaat
     9781 tgatagaaaa attgcacgtc ttcgggcgga atttgttttg ccagaaaatg cactaatgtt
     9841 tcttcggttg taggtgtttt cactaattgc aacattgcaa ttttgtgtaa cacttctgcc
     9901 acatcgctta ataattgttg ccaatcaacg cctttcgctg caatttgctc gatgatcgcc
     9961 attgcttttt caccgtcggc ttgggcggtt gcttgcacta attctagtgg ttgattatcg
    10021 tcaatcaacc ctagcatttg gctgactatc gcaagcgtaa tattggcatt actcattgca
    10081 atggcttgat cggtgaggct gagagagtct cggatactac cttgtgctgc ttttgccaac
    10141 ttttctagtg ccaaaggctc aaacggaatg ttctctgcat ttagaataaa ttcaagatga
    10201 cgggcaattt gcgattgctc taatgcccgc agatgaaatt gcatacagcg agagaggatc
    10261 gtaatcggca gtttttgcgg atcagtggta gccagcaaga atttgacata ttcaggcggc
    10321 tcttcaaggg tttttagtaa ggcgttgaaa ctatgacgag agagcatatg cacttcgtca
    10381 attaaataaa ccttaaaacg cccaacggtg ggtttgtatt gaacgttatc aagtagctca
    10441 cgggtgtctt ccactttagt acgagaagca gcatcaattt caatgagatc gataaaatgc
    10501 ccttcttcaa tcgctttaca attcgcacat tcaccgcaag gctcggcggt gatcccgttt
    10561 acacagttta agcctttggc gaataaacgg gcaatagagg ttttacccac accacgagtg
    10621 cctgaaaaca ggtaagcgtg gtgcagtcta tcttctctta agctgttttc aagagcagat
    10681 aaaacgtgtt gctgaccaac tacttccgag aagcgttgcg gtctccattt acgggctaaa
    10741 acttgataac tcattgattt ttctattcaa tttggaaatc acaagcggtc atctttccga
    10801 aaaattttgc aaaattttag caaaaagcca ccgcttgttc cccattaatg accttcaaag
    10861 ctgactaggc taaaggattt aaccccttct ttttccaaac gctctttacc acctaactca
    10921 ggcagccaaa tcacaaaggc agcgtgttct acgttaccac ctaagcgacg aattagtttt
    10981 gcagttgcat caatagtacc accggttgcg agtaaatcgt cgatgaccaa tacattatca
    11041 ttagcactca ctgcgtcttt atgaatttct agcgtgtctt caccatattc aagcgtgtaa
    11101 gattgcgaaa tcacttcacg tggtaatttt ttcggtttac gaaccaacac aaaaggcacg
    11161 cctaacgcta atgcaacagg ggcaccaaaa ataaacccgc gagattctgt gccgacaatt
    11221 ttagtaatac ctttgtcttt aaattctgca acgatggcat caatggtggc ttggaatgca
    11281 gttggcactt ctaaaagtga agtgatatca cggaagataa tccctgcttt cggataatct
    11341 ggaatggatt tgatagaaga tttaattaaa tctaattggt tcattgtttg acctacgtgt
    11401 aataaaaaaa taactggaaa actctataga aaaacagcag aaatttcaag gagaaattga
    11461 taaccttacg tttgatagta cagcttactt ttcagtttcc ttttccctat attatagata
    11521 aatatcttcc ctgtttcgtc tggttttaag caaacgtaaa atccaaacat actgctcggg
    11581 gtttttctcc acaaaatatt caattactcg gttcatttct cgggcggatt gaattgggtc
    11641 tcctgaaaat tcaagcggtg gcaaaatttc catttcatat tttgcttttt ccgcattata
    11701 aatcgagaac attgggatca cttccgcatt tgccactctt gccattttat ttaagcccgg
    11761 caaagtggct ttttcggtgg caaaaaagtc ggcatacaca cttagctctt cgccgaaatc
    11821 ttcatcgggc agaaaataac caacatcacc tttacgcaca tcggctaaaa acgctttaat
    11881 cccattttga cgagtgtgca ttcgcccacc taaacgctca cgggtaatat tccacagcca
    11941 gtccaccaac gcattacggt gtggattgta cattgatgtc atcggataac cgagcgagaa
    12001 catcgccacc cccgccatat caatcgacca agtatgtggc actaacataa tcacgttttt
    12061 accgctctct ttggctttca ccacattttc aaagccggta aattcacacc gtttttgtaa
    12121 ataagaggcc gggcggattg cagtttcgcc aatcgccaac atggtttgtg ctaccgtgat
    12181 aaacattttc tcgaccactt cttcacgctt tttctcactc caatcgggaa aacagtaacg
    12241 caaattcact ttagcacggt ggtagccttt tttatttttc tgcttgagat agcgatacgc
    12301 catcttaccc acaaaagcgg ataatttatc cctaattcgc accggcacat aggctaaaat
    12361 cactaatcct aatacaccta accatacgcc ccaatatttc gggtgtagaa aagaccatag
    12421 gaatttgtgc gtatagccgt cttgggcggt aattcgtgtc attctataat tcctcattat
    12481 ttgtcacttt ctttgcttcg ccaaagaaag taaccaaaga aaggcaaccc tacttcaccg
    12541 ctatcttcgc ttggtttgaa tttgcttaac gaaaaaattc caaactcgcc gcttcgctgc
    12601 tcaaacaagg aatttttcct acaaattcaa agtcgttcgg gcggttcaga agggaacccg
    12661 gcagagcatc attttgcaaa attttccaaa aaaatcaccg cttgtatggt cggatcgggt
    12721 tcccttctga gttgcccgaa cgacttgaaa tttttaggaa aataactcga gcgagagcga
    12781 agcgaacatc agggttattt ttcgttaaga aaatttcaac caagtgagga tagcaacaaa
    12841 gcagggtgtg ctttcttttg gttacttttc tttgcacaag caaagaaaag tgacataaca
    12901 catcatctct tatcaataat attcttcacc gccaatatca ccccaagtcc aataaaagcc
    12961 cccggaggta aaattgccag tagtaaacct gaatcaatat gcaatacatc aatgcgtagg
    13021 gcttttgccc aatcgccaag gagtagatct aagcctgaaa acagcgtgcc gttgccaata
    13081 atttcacgca acgcgcctag caccacaagg ctaaaagtca ttcccagccc cattgaaaag
    13141 ccgtcaaaag ccgagaattt gacctcattt tttgaggcat aggcttctgc tcgtccaatc
    13201 acgatacagt tggttacaat cagcggaata aaaatcccga gcgattggta aacagggaaa
    13261 gcaaaagcat tcattaaaag ctgtactgcc gttaccaccg ttgcaattac catcacataa
    13321 atcggaatcc gaatatcgtg tggagtgatt ttccgaaaca gggaaaccgt gatattggtg
    13381 caaattagca caaacattgt tgccaagcct agtccaagtg cattggttac gctgtttgaa
    13441 accgccagta gtgggcaaag ccctaataat tgcaccaaag caccattatt tttccacaca
    13501 ccatcagcga aaatcgtttt ccaaacgctg gattgtttcg gggcaacgtc aatattcgtc
    13561 acagggattt gttgttctct ttccatttct ccccctattt gcacgcttta aattgattaa
    13621 acattgtcgg gttttgagcc agctctgtca ccacccattt cgcactttgg cggatatgat
    13681 tgaccaccgc acgaggtgta atagttgcac cggtaaattg gtcgaacttg ccgccatctt
    13741 tttttacatt ccaatccgct tcgttttcta aactaaaaag ttgattggta aaagataaga
    13801 tccaatccga cactcgggtc tcaattttat ctcccagccc cggagtttct ttatgctcaa
    13861 gggtgcgaac gcctaaaatg ttgccgttcg gctcaatccc catcaatagc acaatatcgc
    13921 cggaatagcc gtccggagcc gttccttgaa ctaggtaagc ggtcagattt tccgattttt
    13981 ttgcaacata aatacggttc aaataagggg cattttcgag ctgaaccagt ttgcaagtcg
    14041 ccaacacatc attatcaaaa tggcttttcg gcacaacctc ttccaataat ttgcgttgtt
    14101 gcattgcggt tacatcatca atcttgcctt tggtaagcag gtacacaccg gttgaaacag
    14161 cggtgcaaag caaggcaata aagcctaaaa ttaaggcgta atttaaggta atttttgagg
    14221 tgttcattat ctgttatcca attttgtgcc gtataatctc ggctgcgtat aatggtcgat
    14281 taacggtacg caaatgtttg ccaataacac tgaaaatgca atcgcatcag ggtaatttcc
    14341 gtagtagcgg attagataaa ccaataatcc caccaagcca ccgaaaatca gttttcctct
    14401 tggggtaatg gaggctgtta ccgggtcggt cgcaataaaa aatgccccga acatcatcgc
    14461 accgctaaag aaatggctca caaaatgcaa gtgcgattgt tggctaaaca gctcggtgaa
    14521 tccactaaaa aaggcaaaag ttgccaacat tgctaccgga atttgccaat ggataatccg
    14581 tttataaatc aacaccaaac cgccgaataa gaacgcaagg ttcagttgta gccaaccggt
    14641 cgcaaacagc ccgttgaaaa tcggcgtatt cattaccgcg tcaaaacctg attttgcaag
    14701 gctgcttttc atactatcaa gcggggtagc ttgtgcaatg ccatcaacac tattaactag
    14761 ctgatgaata ctaaaaccat cgctagttac cccgtaaaaa atcaacgaaa tcgcatcacg
    14821 aaatgtcggt ggttcactta ataactcaat cggcactatc cacgccgtca tttgcaccgg
    14881 gaaagaaacc aacaacaacg catagccaac cattgccggg ttaaatagat tctgccccaa
    14941 gccaccataa ctgtgttttg ccaataataa cgccacaatc acgccaatca acacaagcca
    15001 ataaggagca taaggcggaa tcgacatcgc caaaatcaag gcggttaaaa tacctgataa
    15061 gtcagccaaa tagaaagcgg ttgttttttt gcgtaatttt gcaatcccga tttcaatcac
    15121 aatcgcaagg ctaacggcaa gcaacgtttg aatcagcaca ccatagccga aataataaag
    15181 ctgaacccca aaagcaggta acatcatcgc aatcacccac agcataaaat tcgcagtgag
    15241 attgctcgaa tgagtgtggg gggaactgac cattttaaac atctataaaa cctttttagt
    15301 aaaaaattgc aaaaaaatga ccgcttatta aagtctcggc aaattcaaat taaagcgtat
    15361 tgccagcagg cggataataa ttacggtgaa cacggtggca aggtaaaccc aatcgctttc
    15421 gagcccaact gagtttgcag ccacaaaaat cacaccaccg ataaggctgg cggtcacata
    15481 cacttctttt tgcaaaatga tcggcacttc gttgcgtaaa atatcacgga ttaagccacc
    15541 aaaacagcct gtcacaccgc ccagagccac acagatcagt gggtgcaagc cgaaatccaa
    15601 gcctttttgc acgccaatca cggtaaatac gccaagcccg atggcatcaa acaccagcaa
    15661 gccggtttgc caacttttgc gggaaatttg gttgcggaaa atagcggtaa tcaccactgc
    15721 aagggtaatc atcagcacat agatcggttg tttcatccaa aacaccggcg tggagccaat
    15781 catcatatcc cttagcgtac cgcccccgac cccggtcaca aacgctaaaa taaacatacc
    15841 gaaaatgtcc attttgtgct ggcgggcagc aattgccccc gaaatcgcaa acacgatagt
    15901 gccgattaag tcctgaataa acaggaacga ccacggcaat gccggaatta gctcgctaaa
    15961 cattgttgct tttctcctgc tgagcggctt ttttcgcttt agctctggcg atggcggccg
    16021 caacggcggc tttgcgtggg tcttcgcttt ccgttttggg cgaatggtta ttcgccccta
    16081 cagatttatc cgatgtagtt tcgttcattg attgtagggg caaatcatta tttaccccaa
    16141 tcacagcatt ggcaatatct tccggttttt cgaccgcttg ttgagcttgt tttttcgctt
    16201 tagctctagc gatagctgct gcaacggcgg ctttgcgtgg gtcttcgctt tctgtttcgg
    16261 gcgaatggtt attcgcccct acagatttat ccgataccgt ttcgttcatt gattgtaggg
    16321 gcaaatcatt atttacccca atcacggcat tggtaatatt ttctgatttt tcgaccgctt
    16381 gctgtgcctg ttttttggct ttggctcggg caagggcagc cgcaacggcg gctttgcgtg
    16441 ggtcttcgct ttctgcttcg ggcgaatagt tattcgcccc tacagattta tccgatgcag
    16501 tttcgttcat tgattgtaga ggcaaatcat tatttgcccc aatcaaggca tttgtaacat
    16561 tttccgattt ttcgaccgct tgttctgcct gtttggctaa acgtctggct ctgcgttgtg
    16621 ccattaaatc gctgttatca ggctcgccgt taggttttac ctgtacttct gccggtattt
    16681 cttggactgt ttcaaccgct tttttcgctt tcaggcgttc aagagccgct tttacaggat
    16741 caacaccacc tgcatttgcc acttcttcac ggcgtttttc cgccgcttgg ctaatgcgag
    16801 cagttctggc atctcgttct ttttgtaagc gagcttctct cgcctcaaag cggattttag
    16861 cctcttccgc tttgcgggct ttttcctcaa tttcctcgat ttttgctttt tcttggcgga
    16921 aatattggat taacggaatg tagcttgggc aaacgtaagc acaaacgcca cactcaatgc
    16981 aggcatctaa atggtattct ttcgatttat cgtgatcttc tgcacgggca aaccaataaa
    17041 gttgctgtgg caataacccc accgggcaag catcggaaca gcttgaacag cggatacaac
    17101 tacgctccgg ttcaggtggt gcatattcaa aatgatcagg ggcaatcaag cagttcgcag
    17161 ttttggtaat cggggcttgc aatgcaggca aaataaagcc catcatcggt ccgcctaaaa
    17221 acaccggaaa gcgtttatcg gcttgataat ctacctgctc caacaagtgc tgaattggcg
    17281 tgcctaatct tgcccacacg ttccccttgt tgcgaatttt atcgcctgtc agcgtcacta
    17341 cccgttcaat caaaggttca tcatcaataa ttgcccgttt aaccgcaaac gccgtgccga
    17401 cattgtgcat cacaatgccc atttcaatag tgcgtttgcc agccggaatc tcaagtccgg
    17461 tcaacacttg aatcagctga tcagaagctc cggagggata tttagtcggg atcgcccgca
    17521 caaacatatc gttcgagcct tttaaagcgg tttctaaagc ttgaatagcg tgcggtttgt
    17581 tgtcttcaat cgcaatcacc acttcttccg ggcgaagcac ataacgcaga atgcgaatac
    17641 cttcgataag atccgcagtg aaatcttgca ttaagcgatc gtcacaggtg atgtaaggct
    17701 cacattctgc cccgttaatc accagtaatt tacagcgttt atccgccaaa cttaattttg
    17761 ttgcggttgg gaataccgca ccgcctaaac cggcaatgcc atattggtaa actttttgga
    17821 taatttgatc gcttgttagg cttaaaaaat cctcaatcgg ctggcgttcc acccatttat
    17881 ctttaccatc ggtttcaata atcaccgtta tttcgggcaa acctgaggcg tgagcggaaa
    17941 cataaggctc aatcccaatc acccgcccgg aggttgggct gtgtacgggc agttgacgga
    18001 agtgatcgcc tttcgtgagc ggctgccctt tcaataccac atcgccgact ttgactaata
    18061 attcccctgc ccagccagaa tgttgaacca gtggcacata gaaaaacttc ggtaaattta
    18121 aggtgcgaat tggcttgctg tttgattggg atttattttc catcgggtga ataccgcccg
    18181 ggaaatccca cagtttaccc gtttctttat tttggcggat tcgctcaatc actaaattag
    18241 gattgctcat ttttgcctcc gccggtaact aattttctct gcaattcagt ggtattcaca
    18301 atcggaataa ttaaatcttg gtcaaatttc cagttccaag attgggttgt cggtttaatt
    18361 ggtcgcattt caatacagtt ggtcgggcaa ggggcgacac aaagttcaca gccggtgcaa
    18421 agatccggaa taatggtgtg cattgcttta ttcgtgccga taatcgcatc aaccgggcaa
    18481 gcctgaatgc acttagtaca gccaatgcag agatcttcaa tcacaaaagc cactttcatc
    18541 tcaggctctg ccataccttc cattggcggc acttctaccc caagcaattc ggcaagattc
    18601 accactaaag gttgtccacc cggcacacat ttagtgatca catcaccatt agcaatcgcc
    18661 tcagcataag gcttacagcc cgggtagcca cattgaccgc attggctttg tggtaaaagg
    18721 gcatcaattt gatcgacaat cgggtcggat tcaactttca gtttaatcga ggaatagcct
    18781 aaaaccgccc cgaaaatcag tgcgatcaca gcaatagcga taagaatata agcgataatt
    18841 ggcaaatcaa gcattagagt ttcaccaatc ctgtaaagcc cataaaggca agcgacatca
    18901 agcctgcggt aatcagagca attgatgctc cttggaacgg acgaggcaca tcagcggcag
    18961 ccaaacgctc acgcagagcc gcaaataaaa ctaacaccag cgagaaaccg gcagcggctc
    19021 caaaaccata aagaacagat tccaccaagt tattggctaa attcacgttt aataatgcca
    19081 cccctaatac ggcacagttg gtggtaatca gcggtaaata gatcccaagc aagcgataaa
    19141 gtgtcgggct ggttttatgc acaatcattt ccgttaattg cacgatcacc gcaatcacta
    19201 aaataaacac caaggtacgc aaaaattgag cgtttaacgg ctcaagcaaa taggtttcca
    19261 tcaaataagc agagagcgag gcaaccgtca gcacaaaggt tgtcgccaac cccataccaa
    19321 tagcagtttc cacctttttc gacactccca taaacggaca aagccctaga aatttaacta
    19381 agacaaagtt attaataagg gcggtgctga tgaccaacag aatataatcg accataaatt
    19441 ccacaaaatt aaatatacaa gcggtcaaaa acgcatttta ttttgcaaaa aacagagaaa
    19501 atctgaccgt ttgttaaagc atctcattaa aaataaacgt tatattatct cttgttttcg
    19561 gctcattcac aataagaaat cggaggtact tctagtaaac ataccatatc aaatatgatc
    19621 ttgttacctt tgtttctatc tctggtagaa tggagaatgt ggggacta
//
LOCUS       DANLXS010000024        19356 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.024, whole genome shotgun sequence.
ACCESSION   DANLXS010000024 DANLXS010000000
VERSION     DANLXS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19356
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.024"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..2519
                     /gene="adhE"
                     /locus_tag="PXD71_000682"
                     /gene_synonym="adhC"
     CDS             <1..2519
                     /gene="adhE"
                     /locus_tag="PXD71_000682"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL6290836.1"
                     /translation="NQEQVDFIVAKASVAALDKHGILAMHAYEETGRGVFEDKATKNL
                     FACEYVVNNMRHLKTAGVISEDDVTGITEIADPVGVVCGITPTTNPTSTTIFKALIAL
                     KTRNPIVFAFHPSAQQSSAHAAQIVRDAAVAAGAPENCIQWITQPSMEGTSALMKHPG
                     IATILATGGNAMVEAAYSCGKPALGVGAGNVPAYVEKTAKLKQAVYDIVMSKSFDNGM
                     ICASEQAAIVDKEIYAEFIKEMQSYGVYLVNKKEKALLEKYIFGIDKAKDENCSGAKL
                     NSAVVGKPAAWIAEQAGFKVPEKTNILLAECAFVGEAEPLTREKLSPVLALLKSDSTE
                     HGLELSEAMVNFHGLGHSAAIHTENKALAKTFGERVKAIRVIWNSPSTFGGIGDVYNS
                     FLPSLTLGCGSYGKNSVGNNVSAVNLINIKRVGRRRNNMQWFKVPSKIYFERDSIQYL
                     KSMHDVHKVMIVTDRSMVDLGFVDRITEQLRQRRNQVMIQFFTDVEPNPSLQTVQRGT
                     ELMRSFQPDTIIALGGGSPMDAAKIMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPQLG
                     RKAKFVGIPTTSGTGSEVTPFAVITDGDIKYPLADYSLTPTIAIVDPALVMSVPSHVA
                     ADTGLDVLTHATEAYTSILANDYTDGLALQAIKLVFENLEKSVKEFDETAREKMHNAS
                     TMAGMAFANAFLGICHSMAHKIGGKFHTIHGRTNAILLPHVIRYNGTRPTKVATWPKY
                     TNYVADVRFQDIARMLGLPAETPEQAVEAYAQAVHDLAVRCGVKMSLREQGVDEQAFL
                     NARRELALHAFEDQCTPANPRLAMIEDMEAIMTKAYYGKE"
     gene            2666..3562
                     /locus_tag="PXD71_000683"
     CDS             2666..3562
                     /locus_tag="PXD71_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDL6290837.1"
                     /translation="MQITILDGGISRELMRRNAPFHQPEWSAAALYEGPHFVQAVHED
                     FIAHGAEVITTDSYAVVPFHIGEKRFAADGKMLADLAGRLAKQAVKNSGNIATQIAGS
                     LPPMFGSYRADLIQMERFAEVAQPLIDGLSPYVDIWLCETQSAIIEPISIKALLPKDN
                     RPLWVSFTLTDDEPTTEPQLRSGESVKQAVEEMVKLGVQAILFNCCQPEVIGEALSVT
                     QKTLAELKATHIRTGAYANAFAPQPKDATANDGLDEVRQDLTLEAYLGWAKKWTEQGA
                     TIIGGCCGIGVEYIETLAKNLK"
     gene            3572..4981
                     /locus_tag="PXD71_000684"
     CDS             3572..4981
                     /locus_tag="PXD71_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL6290838.1"
                     /translation="MSNKKIGLISLTALVLSSMIGSGIFSLPQNMAAVAGAEAITIGW
                     LITGVGIIFLGLSFFFISRLRPDLDGGIYTYAREGFGELMGFMSAWGYWLCATIGIVG
                     YLTVAFEGLGVFTDNPNTVIFGQGNTLASFIGSSIIVWLVHALIAGGIKEAAFVNLIA
                     TFVKVAPLILFIGLGIWYFDVDLFNSDVKATALNNSVSDQVKSTMLITLWVFTGVEGA
                     SVLSAHAKKRSDVGLATVLGIIIALALYVAITILSLGILPRETIANMANPSMGPLLDA
                     MMGPTGKVIITACLIVSVLASYISWTMYSAEVPYRGSKNGAFPKILDKVNKNGTPINS
                     LWFTGFIVQFCLVLVFVFEQSYNTLLLISTSMILIPYFLIGAYLFKLAIQTNATWYIK
                     LTGFMAALYGLWIVYAAGLEYLLLSVVLYVPGILLFLYSNYKFNGRLTLSTAEKVILI
                     VLLVIFGYAVIELPALLAA"
     gene            5088..6311
                     /locus_tag="PXD71_000685"
     CDS             5088..6311
                     /locus_tag="PXD71_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HDL6290839.1"
                     /translation="MQTYQHKALCRVRTITLFLSLTKDKLQWETALNAAKAEFDLLVP
                     AIQTQGYHIQSIRIVTNSFGEYLDLTNIETAKQDLAYLKQLLTQLNDSGLRIRFAIGE
                     AKTPHELALLPELILNYGDLSNACVNIEPDEFGILDNETILQSAQIIKQIAEITPRGE
                     GNFNFTVNFNCKPFIPYFPASYHSSKLPNSFVIGLETPDLLVAVLKELKASPHNDFYR
                     QCYETMSQALQYHVDEVLEAVKSVQHKLQFAFAGIDSSAAPSKNCASMTEVYELMGVP
                     FFGAAGTVEASSLLTKVFKSIKHVPLVGFSGLMLAVTEDLGLAAGTHKAQFDIRALLT
                     YSAVCGIGLDTVPISNEATVEQIAALMRDTGTMAFRLNKPLTVRLFPIPNKSAGEVTE
                     FESDDLCNCRILAVP"
     gene            complement(6349..7545)
                     /locus_tag="PXD71_000686"
     CDS             complement(6349..7545)
                     /locus_tag="PXD71_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL6290840.1"
                     /translation="MKQVIRFIVFSTLSLFSFSTFAKTIVAIDAGHGGKDPGAIGKNL
                     GILEKSVTLAISKELKALLDADPNFKAVMTRSGDYFIQLPQRTEIARKHRADLLVSIH
                     ADSSPRGDSLKGASVWVLSNSRASDEMGKWLEDHEKQSELLGGAGSVLSSNNERYLNQ
                     TVLDLQFSHSQRAGYELGKSILAKMSNVAALAKSSPQHASLSVLRSPDIPSVLVETGF
                     LSNSTEEAKLASANYRKQVARAIYNGLVAYRAKNAVASSSVSKKSDKEDKQNKKEEKT
                     AKADLTKKPASEKQEKNTQSDKTKLKEKSKKEDKKEDSAKTNKKKEQQLKEKTALEKD
                     QKNQSKKSDSQREIKVSNSGYHIVQQDETVYSIARAYGTTPQKISELNNLKNYQITVG
                     KKLKVK"
     gene            complement(7565..8056)
                     /gene="tsaE"
                     /locus_tag="PXD71_000687"
     CDS             complement(7565..8056)
                     /gene="tsaE"
                     /locus_tag="PXD71_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL6290841.1"
                     /translation="MTDSLSFYCEDETKLLEFGQSFALELKKYLLQDQAHSLVVYLNG
                     ELGAGKTTLTRSIVRAFGHQGNVKSPTYTLVEEYQLPPFSLYHFDLYRLADPEELEFM
                     GIRDYFKPQTLCLLEWAVKGKGMIPEADFVIQIDYKNDGRQISLLPQNQTAVDILVNF
                     HQK"
     gene            complement(8056..8610)
                     /gene="orn"
                     /locus_tag="PXD71_000688"
     CDS             complement(8056..8610)
                     /gene="orn"
                     /locus_tag="PXD71_000688"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDL6290842.1"
                     /translation="MAQNEQNLIWIDLEMTGLDPEKERIIEIATIVTDKDLNILAEGP
                     VLAVHQTDDLLEKMSDWCVKTHTANGLIERVKASKLTERAAELQTLDFLKRWVPKGAS
                     PICGNSIAQDKRFLYKYMPDLADYFHYRHLDVSTLKELARRWKPEILDKFSKGNTHLA
                     LDDIRESINELKFYREHFIQLDQK"
     gene            complement(8620..9099)
                     /gene="coaD"
                     /locus_tag="PXD71_000689"
     CDS             complement(8620..9099)
                     /gene="coaD"
                     /locus_tag="PXD71_000689"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL6290843.1"
                     /translation="MSYNVIYAGTFDPITNGHLDIICKASHLFGKVIVAVAQNPSKQP
                     LFSLEERVELVKQSCTQWQNIEVIGFSGLLADFAKQQNAKALIRGIRGADDIDYEIQL
                     AQLNYKLSGDLETIFFPPSIEWRYLSSTMVREIYRHNGDISQFVPQAVKNALKIAKV"
     gene            complement(9101..10381)
                     /gene="waaA"
                     /locus_tag="PXD71_000690"
     CDS             complement(9101..10381)
                     /gene="waaA"
                     /locus_tag="PXD71_000690"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDL6290844.1"
                     /translation="MLRLLYICLSYLLQPVVLLLMWYKGRKQPAYRKRLWERYGIYDE
                     SEKPKAKGVVIHAASVGEVIAATLLIKAVSKQYPELPLIVTTVTPTGSGRVQAAFGNS
                     VSHFYLPYDLPDAIERFLNFIDPKLMIVIETELWPNLIRKVNIRKIPFVIANARLSPR
                     SAKRYGWVKGSIKDMLNQISLIMAQDEVSRDRYLALGYAPAKMVNTGNLKFDLEITPQ
                     LHQSVIKTKASLNLTERPIWIAGSTHEGEEKLILDAHKLLLEHYPNLVLILVPRHPER
                     FGLVELLIQKSDLNYVKRTENKTLSQNTQVMLGDTMGEMMLLYGLSDIAFVGGSLVKH
                     GGHNPLEPIAFNIPVVSGLYTYNFPEIFEKLREVKGVIEIESSVEALTESIQLLLKQP
                     QIGQEIAKSGLSVLQENQGALKRHLDLLASYLEK"
     gene            complement(10561..11940)
                     /gene="yegQ"
                     /locus_tag="PXD71_000691"
     CDS             complement(10561..11940)
                     /gene="yegQ"
                     /locus_tag="PXD71_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDL6290845.1"
                     /translation="MTLKYAKPELLSPAGSLKNMRYAFAYGADAIYAGQPRYSLRVRN
                     NEFNHANLKVAIDEAHALGKKFYVVVNIAPHNSKLKTFIKDLKVVVDMKPDALIMSDP
                     GLIMLVRENFPEMDIHLSVQANAVNWATVKFWRQMGLTRVILSRELSLEEIEEIREQV
                     PDMELEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYKMEEGKIDEVGQI
                     VPKYEPEIEVKNVAPTLGNGEATDKVFLYTESQRPDEQMTAFEDEHGTYFMNSKDLRA
                     VQHVERLTQMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDESLMDTLESL
                     AHRGYTEGFLRRHTHDEYQNYEYGYSISERQQFVGEFTGKRNAEGLAEVAVKNKFLVG
                     DSVEMMTPKGNIVFKIERMLNRKNENVDAALGDGHFVFLDVPADIELDFALLMRNLVN
                     TNTRNPHQA"
     gene            12068..12439
                     /locus_tag="PXD71_000692"
     CDS             12068..12439
                     /locus_tag="PXD71_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290846.1"
                     /translation="MKLKYLLATTLLAFSTFTAAQSIKTEMMQMNMQANKLMRAESAE
                     EFSEIANDFIIITEKTKGIFPNSVEGDKAAQEDYQAGMQKLIDMVNQASEKAQAGELQ
                     EAKMLISELEMIKREYHKKYK"
     gene            complement(12478..15954)
                     /gene="dnaE"
                     /locus_tag="PXD71_000693"
     CDS             complement(12478..15954)
                     /gene="dnaE"
                     /locus_tag="PXD71_000693"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL6290847.1"
                     /translation="MSDCRFVHLKVHSDFSMINGLAKVKSLVKTAAANNMVAMGLTDF
                     INFCGLVKFYGEALGAGIKPIIGADVYVRTESGNEDYFELTLLAKNNKGYHNITQILS
                     KAYQQGYVEFPMVEREWLAELNEGIIILSGGRMGDVGKALLKENRQEAEEKLAFYQQY
                     YPNHFYLSVCRTGRADEERYIKQAVEFSQKNAIPLVAVNDVVFLKEDDFDAHEIRVAI
                     HDSYTLDDPKRPKKYSPKQYFRTEEEMCKLFADLPSALANTVEIAKRCSVTVRLGEYF
                     LPNFPTGELSTEDFLVKKSKEGLEERLEFLFPDPEERANKRSVYDERLQVELDVINQM
                     GFPGYFLIVMEFIQWSKDNDIPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLN
                     PERVSMPDFDVDFCMDGRDRVIEHVAETYGRQAVSQIITFGTMAAKAVIRDVGRVLGH
                     PYSFVDRISKLIPPDPGMTLEKAFAAEPKLPELYEADEEVKDLIDMARKLEGVTRNAG
                     KHAGGVVIAPTAITDFSPLYCDSEGLHPVTHFDKNDVEYAGLVKFDFLGLRTLTIIKW
                     ALEMINERLAREGKEPIRIETIPLDDKKSFALLLAAKTTAVFQLESRGMKDLISRLKP
                     DCFEDIIALVALFRPGPLESGMVQNFIDRKHGKEEVSYPDPQYQHECLKPILEPTYGV
                     IVYQEQVMQIAQELAGYTLGGADLLRRAMGKKKPEEMAAQREVFEKGAEAKGIDGKLA
                     GQIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTSEMDNTDKIVG
                     FYDECMNMGLTVVPPDVNSGKHRFSVNEKGEIVYGLGAIKGVGEGPIEAILAARQKEG
                     KFKDLFDLTARVDLKKINRRTFESLIMSGAFDKLGPHRAALMKNLEDALKASDQHAKM
                     EELGQSDMFGVLTETPEEVQNAYANTPKWSEQVILDGERATLGLYLSGHPIGRFLKEL
                     SHYAPNRLNELQPTFRGQMTTVSGIVMASRVAVTKRGSRIGIATIEDRSGKLDITLFS
                     EALENFGHLMQQDQIIVATGSVQFDDFSGGLKMSAREVLSLDEARGRFAKSLALAISK
                     EQLSADFIKKMTAIITPHKEGTLPLHFYYQSPEGRVLLKGGVEWRISPNEAMFSELKA
                     LLGENSVELEFE"
     gene            complement(16179..17276)
                     /gene="lptG"
                     /locus_tag="PXD71_000694"
     CDS             complement(16179..17276)
                     /gene="lptG"
                     /locus_tag="PXD71_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDL6290848.1"
                     /translation="MQFFSMNILERYIGKTILSAIFLTLFMLIGLGAIIKFVEEFRDV
                     GRGSYDGLKAAYYTFLTIPRDIETFFPIAALLGSLLGLGNLASLSELVVMQASGFSRF
                     RIGLAVMKTALPLVIFTMIIGEWGVPQTEQFARNMRSIAQSGGSMLATKDGFWAKDGN
                     SFIYINHIKNEKSLDNVLIYQFGEGQNKRELQSVLKARTANYSDEKGWVLSKVEKSTV
                     GENQIQQAKQPDQVWQTSLTPSKLGIVSLKPESLSISGLADYVGFLKDTGQDPKRFEI
                     TFWRKVFQPISMAVMMLLAISFIFGPLRSSTMGAKIVIGIVAGFMFYVANIVFGNLSL
                     IATWLPIPVGALIPSLLCLIIVWWLLSKKRD"
     gene            complement(17276..18385)
                     /gene="lptF"
                     /locus_tag="PXD71_000695"
     CDS             complement(17276..18385)
                     /gene="lptF"
                     /locus_tag="PXD71_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDL6290849.1"
                     /translation="MILSRYLTKEIFKSQLAILFILLLIFFCQQLVRVLSSAVSGKVP
                     ADLVVSLLGLGMPTMAQLMLPLSLFIALLLTLGRFYAESEITVMRACGIGQGLLVKVA
                     MFLSIFTTALAVYNVFWLTPWAINKQSEMLAEAKANPRFAALSAGQFMSAGGYVLFID
                     KIENNSLNDIYVFQPEQQKKNKPSVVVAESGTLQGLPNGDQVLTLAESKRFEGTPQSA
                     DFRISHFENYSAYLGYQDVESDEKLVQRADFKKLMETKTPEAKAELHWRLALILAVPL
                     MALLAVPMSSVNPRQGRFAKLLPALLLYLIYFLLQSSLKSSGASGKLNAEVLMPLVSV
                     AFLVLGVILNAWDSKFMSKLRYQFGAKKLTNKAVA"
     gene            complement(18559..19356)
                     /locus_tag="PXD71_000696"
     CDS             complement(18559..19356)
                     /locus_tag="PXD71_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290850.1"
                     /translation="MRFWGKTFVVGLIGLGTQNTIFANDDAQISRYEQAKLRLSYDGN
                     VGNLLQQLSQRLKVGFIAYDVDITRKISLQNETETAIKTINEQIESQLTNIDVRFEKI
                     GERLFLVVSAKDVAPLLQNEPKEVQFVGDVIFDGEPNLTEQAQSTAEATKVQNILNIA
                     TNQEKIMAAKGKKAPQYKTVTKDNIGLKNIRVTALGTFLVFDTSVDAAKLRVKGNFED
                     IAQGENIIAILHQNTEAPPKIEIEDSQGKKLILEAHKATSSKKSKKQ"
BASE COUNT         5511 a         4416 c         3773 g         5656 t
ORIGIN      
        1 tcaaccaaga acaagtggat tttattgtcg ctaaagcctc tgttgctgcc cttgataaac
       61 acggtatttt agcaatgcac gcttacgaag aaaccggacg gggtgtgttt gaagacaaag
      121 ccactaaaaa cctgttcgcc tgtgagtatg ttgtcaataa tatgcgacat ttgaaaaccg
      181 caggggtcat tagtgaagat gatgtcaccg gtattaccga aattgccgac cctgtcggtg
      241 ttgtctgcgg tatcacccca accaccaacc caacctcaac caccattttc aaagcgttaa
      301 tcgcccttaa aacccgtaac cctatcgttt ttgctttcca cccgtctgct caacaatctt
      361 ctgcccacgc tgctcaaatt gtgcgtgatg ctgccgtggc tgccggtgca ccggaaaact
      421 gtattcaatg gattacccag ccttcaatgg aaggtacatc tgctttaatg aaacacccgg
      481 gcattgcaac tattttagcc accggcggta atgcaatggt tgaagccgct tactcttgcg
      541 gcaaaccggc attaggagtt ggtgcaggca acgtaccggc ttatgtagaa aaaaccgcta
      601 aattaaaaca agcggtgtat gacattgtaa tgtcaaaatc tttcgacaac ggtatgattt
      661 gtgcctctga acaagcggcg attgtagata aagaaattta cgctgagttt attaaagaga
      721 tgcaatctta cggcgtctat ttagtgaaca aaaaagagaa agccttattg gaaaaataca
      781 ttttcggtat tgataaagcc aaagatgaaa actgctcagg tgcaaaactc aattcagcgg
      841 tggttggcaa accggctgcg tggattgcag aacaagccgg ctttaaagta ccggaaaaaa
      901 ccaatattct gttagccgaa tgtgcctttg tgggcgaagc tgagccactt acccgtgaaa
      961 aactttcgcc tgtactggct ctgttaaaat ccgactccac cgaacacggt ttggaattgt
     1021 ctgaagcgat ggtgaatttc cacggtttag ggcatagtgc cgccattcat accgaaaaca
     1081 aagccttagc caaaaccttt ggcgaacggg taaaagccat tcgggtgatt tggaactcac
     1141 catcaacctt tggcggtatc ggtgatgtat ataactcctt cctgccatcg cttacgctgg
     1201 gctgtggttc ctacggtaaa aactctgtag gtaacaacgt tagtgcggtg aatttaatca
     1261 atatcaaacg tgtgggcaga cggagaaata atatgcaatg gtttaaagtg ccatcaaaaa
     1321 tctactttga gcgtgactca attcaatatt tgaaatcaat gcacgatgtg cataaagtaa
     1381 tgatcgtaac cgaccgctca atggttgatc tcggctttgt ggatagaatt accgaacaac
     1441 ttcgccaacg tcgcaaccaa gtgatgattc agttctttac cgatgttgaa ccaaacccaa
     1501 gcctgcaaac cgtacaacgt ggtacagaat taatgcgtag cttccagcct gatactatca
     1561 tcgcacttgg tggtggttca ccaatggatg cagccaaaat tatgtggtta ttctacgaac
     1621 aaccggaagt cgatttccgt gatttagtgc aaaaatttat ggatatccgc aaacgtgcat
     1681 tcaaattccc acaattaggc agaaaagcga aatttgtcgg cattccaacc acctccggaa
     1741 caggctctga agtcacaccg tttgcagtaa ttaccgatgg cgatattaaa tatccgctcg
     1801 ctgactactc gcttacccca accattgcga ttgtcgatcc tgcgttagta atgtctgtgc
     1861 cgtctcacgt tgctgccgac accggtttag acgtattaac tcacgctacc gaggcttaca
     1921 catcgatttt agccaacgac tataccgacg gcttagcctt gcaagccatt aagttagtgt
     1981 ttgaaaactt ggaaaaatcg gtcaaagagt ttgatgaaac cgcccgtgaa aaaatgcaca
     2041 atgcttccac aatggcaggg atggcatttg ccaatgcctt cttaggtatt tgccactcaa
     2101 tggcacacaa aatcgggggt aaattccaca ccatccacgg tcgaaccaat gcgattttac
     2161 tgccacacgt tattcgctat aacggcacac gcccaaccaa agtggcaact tggccaaaat
     2221 acaccaatta tgtcgcagat gtgcgtttcc aagatattgc cagaatgctt ggcttaccgg
     2281 cagaaacccc ggaacaagcg gtggaagctt acgcccaagc ggtacacgat ttagccgttc
     2341 gttgcggcgt gaaaatgtcg cttcgtgagc aaggcgttga tgagcaagcc ttcttgaatg
     2401 cccgccgtga acttgcccta cacgcatttg aagaccaatg cacaccagca aacccacgtt
     2461 tggcaatgat tgaagatatg gaagcgatta tgactaaagc ctactatggc aaggaataaa
     2521 gcacaattta atcgttaaac ttactaaatg gccgcttgca agcggtcatt tttcttgaaa
     2581 tttttacttt tcccttcaca aaatactttc gctcttttac aaagcatagt atttttatcg
     2641 ctccttttat cttacaggaa atactatgca aattacgatt ttagacggcg gaattagccg
     2701 tgaattaatg cgtcgcaatg ctccatttca tcagcctgaa tggtctgctg ccgctttgta
     2761 tgaaggccca cattttgtgc aagcggtaca cgaagatttt atcgctcacg gggcagaagt
     2821 gattactacc gacagctatg cggttgtgcc gtttcacatt ggcgaaaaac gctttgcggc
     2881 agacggcaaa atgcttgccg atttagccgg tcgccttgcc aaacaagcgg tcaaaaattc
     2941 aggaaatatt gcaactcaaa ttgcaggctc acttccacca atgtttggct cttaccgtgc
     3001 cgatttaatt caaatggaac gctttgctga agtcgctcag cctttaattg acgggctttc
     3061 gccttatgtg gatatttggc tttgcgaaac ccaaagtgcg attattgagc cgatttccat
     3121 caaagcatta ttgccgaaag acaaccgccc gctttgggtc tcttttaccc tcaccgatga
     3181 tgaaccaacc accgagccac aacttcgctc gggcgaaagc gttaaacaag cagtcgaaga
     3241 aatggtgaag ttaggcgtgc aagcgatttt atttaactgc tgtcagcctg aagtgatcgg
     3301 cgaggcatta agtgtaaccc aaaaaacttt agccgaatta aaagctaccc atattcgcac
     3361 aggagcttat gccaacgcct ttgctccaca accaaaagat gccaccgcca atgacggttt
     3421 agatgaagtt cgccaagatt taaccctgga ggcttattta ggctgggcaa aaaaatggac
     3481 ggaacaaggg gcaactatta tcggtggctg ctgcggcatt ggggttgagt atattgaaac
     3541 gctggcaaaa aatttaaaat aaggaaatcg aatgtcaaat aaaaaaatag gattaatttc
     3601 actcaccgcc ttagtgctaa gctcaatgat tggctcgggg attttcagct taccgcaaaa
     3661 tatggcagcc gttgccggag cagaagccat taccattggc tggctgatta ccggtgtcgg
     3721 cattatcttt ttaggtttat cgtttttctt tatttcccgc ttgcgtccgg atttagacgg
     3781 tggcatttac acctacgcac gggagggttt tggcgagctg atgggcttta tgtcagcgtg
     3841 gggctattgg ctctgtgcca ccattgggat tgtaggatac ctcaccgttg cttttgaagg
     3901 tttgggtgta tttaccgaca acccgaacac cgtgattttc gggcaaggta acaccctcgc
     3961 ctcttttatt ggatcttcaa tcattgtgtg gctggttcac gccctcatcg caggcggtat
     4021 caaagaagct gcctttgtaa acttaattgc cacttttgtg aaagtcgctc cgctcattct
     4081 gtttatcggg ttaggcattt ggtatttcga tgtggatctg tttaatagcg atgttaaagc
     4141 caccgcactt aataatagtg taagcgacca agtgaaaagc acaatgttaa tcaccttatg
     4201 ggtatttacc ggtgtggaag gggcttctgt gctgtcggct cacgcgaaaa agcgttctga
     4261 cgtgggactg gcaaccgtat taggtattat tattgcactt gctctttatg tggcaattac
     4321 cattctttca ctcggaattc tgccacggga aactattgcc aatatggcaa atccgtcaat
     4381 ggggccgttg ctcgatgcaa tgatgggccc aaccggcaaa gtgattatta ctgcttgttt
     4441 aattgtatcg gtgcttgcct cttacattag ctggacgatg tattcggcag aagtgcctta
     4501 tcgtggctca aaaaacgggg ctttcccgaa aattttagac aaagtgaata aaaacggcac
     4561 gccgattaac tcactctggt ttaccggctt tatcgtgcaa ttttgcttgg tgttggtgtt
     4621 tgtttttgag caaagctata acaccttatt gctgatttcc acctcgatga ttttaattcc
     4681 ctatttctta atcggggctt atttattcaa attagcgatt caaaccaacg ccacttggta
     4741 cattaaactc accggcttta tggcagccct ttacggatta tggattgtct atgccgcagg
     4801 actagaatat ttgctgcttt cggtggtgct ttatgtaccc ggaattttac tcttcctcta
     4861 ctccaactat aaattcaacg gcagattaac cctttcaacc gctgaaaagg tcatcttaat
     4921 cgtgctactg gtaatttttg gctatgccgt gattgagctg ccagcactat tagcagcata
     4981 attcccatca aaaatgaccg cttgcaagcg gttatttttc taggatcttc tgcaaagatt
     5041 cattagaata gaaacaacgt caataataaa taaaaagagg agaaacaatg caaacttatc
     5101 aacataaagc cctttgtcgg gtaagaacca ttaccctttt tctgagttta accaaagata
     5161 aattgcagtg ggaaacggca ttaaatgcgg caaaagcaga atttgatcta ttagttcccg
     5221 ctattcaaac acaaggctat cacatacaat cgattcgaat cgttaccaac agtttcgggg
     5281 aatatcttga tctcactaat attgaaacag cgaaacagga tttagcctat cttaaacagc
     5341 tattaaccca acttaacgat agcggtttac gcattcgctt tgccattggt gaggcaaaaa
     5401 caccccacga attagcatta ctacctgagc tgattttaaa ttatggtgat ttatctaacg
     5461 cttgtgtcaa tattgaaccc gatgaatttg ggattttaga caatgaaacc attttacaat
     5521 ctgctcaaat tatcaaacaa atcgctgaaa ttaccccaag aggtgaaggc aattttaatt
     5581 ttacggtgaa ttttaactgt aaacctttta ttccttattt tccggcaagc taccattcaa
     5641 gcaagctccc taacagtttt gtgataggcc tagaaacacc agatctatta gtagcggtgt
     5701 taaaagagct gaaagccagc ccacataacg acttttatcg ccaatgctat gaaacgatgt
     5761 ctcaagcctt gcaatatcac gttgatgaag ttctcgaagc agtaaaatcg gtacaacaca
     5821 aattacaatt tgcctttgct ggcattgata gttctgctgc tccgtcaaaa aactgtgcct
     5881 caatgacaga agtttatgaa ttaatgggcg taccattttt tggggcagcc ggaacagtag
     5941 aagcctcttc acttctgaca aaagtcttta aatctatcaa acatgtaccg cttgtcggtt
     6001 tttcaggttt aatgttggca gtaaccgaag atttaggctt agccgcaggc acacacaaag
     6061 cccaattcga cattcgtgct ttactaactt atagtgctgt ttgtggcatt gggttagata
     6121 ccgtaccgat ttccaatgaa gccactgttg aacaaattgc cgcattaatg cgagacaccg
     6181 gcacaatggc atttagacta aataaaccgc ttacagtacg tttatttcca attccgaata
     6241 agagtgctgg ggaagtgaca gaatttgaaa gtgatgattt atgtaactgc cgaattttag
     6301 ctgtacctta aaataaaggc tgatggatat ttccatcagc ctttcaattt acttcacctt
     6361 caatttttta cctaccgtga tctgataatt tttcaaatta ttcagctcac tgattttttg
     6421 cggcgtagta ccgtaagctc tagcaatcga gtaaaccgtt tcatcttgtt ggacaatatg
     6481 gtagccactg ttagacacct taatttctct ttgagagtct gattttttag actgattttt
     6541 ttgatctttc tcaagagctg ttttttcttt tagttgctgc tcttttttct tattggtttt
     6601 agccgaatct tcttttttat cctctttttt gctcttttct ttcaattttg tcttatctga
     6661 ttgcgtattc ttctcttgtt tctcagaagc aggcttttta gttaaatcag ctttagcagt
     6721 tttttcctct ttcttatttt gcttatcttc tttgtccgat tttttactga ccgaagaact
     6781 agcaactgca ttttttgccc gataagcgac taatccatta taaatggccc gagcaacttg
     6841 tttacgataa tttgcactgg ctagctttgc ttcctcggtt gaattagata aaaatcccgt
     6901 ttccaccaat acagatggaa tatcaggtga acgcagaaca cttaagcttg cgtgctgagg
     6961 agacgatttt gctaatgcag ccacatttga cattttagct aaaatgctct tgcctaattc
     7021 atagccggca cgttgagagt gagagaactg taaatcaagc actgtttgat ttaaataacg
     7081 ttcattgttg cttgaaagca ccgaaccagc cccgccaagt aattccgact gcttctcgtg
     7141 gtcttccaac catttaccca tttcatcgct agcacggcta ttagaaagta cccaaaccga
     7201 agcccctttc aaggaatcgc cacgaggcga agagtctgcg tgaatagaaa ccagcagatc
     7261 ggcacgatgc ttacgggcaa tctcggtacg ttgcggtaat tggataaaat aatctcccga
     7321 gcgtgtcatc actgctttaa aattaggatc ggcatctaac aaggctttta gctctttaga
     7381 aattgcgagg gtaacgcttt tttccaaaat ccccagattt ttaccaatag cccccggatc
     7441 ttttcccccg tgtccggcat caatcgccac aatggttttg gcaaatgtgg aaaaactgaa
     7501 taaactcaat gtgctaaaaa caataaatct gataacttgc ttcattacct cccctacaag
     7561 cggtctattt ttgatgaaaa tttactaata tatctactgc agtttgattt tgcggcaaca
     7621 ggctaatctg ccgtccgtca ttcttataat caatttggat cacaaaatca gcctctggaa
     7681 tcattccttt acctttaact gcccattcaa gcaaacaaag cgtttgtggt ttgaaataat
     7741 cacgaattcc cataaactcc agctcttcag gatcggctaa gcgatacaaa tcaaaatggt
     7801 aaaggctgaa aggcggaagt tggtattctt ccaccagagt ataagttggg ctttttacat
     7861 tgccttgatg cccgaaagcc cgtacgatcg agcgggttaa ggtggtttta cctgccccaa
     7921 gttcgccgtt taggtaaaca accagcgaat gagcttggtc ttgcaataaa tatttcttaa
     7981 gttctaacgc gaatgattga ccaaattcca gcaatttagt ttcatcttcg cagtaaaaag
     8041 agagagaatc tgtcattatt tttggtctag ctgaataaaa tgttcccgat aaaatttcaa
     8101 ttcgttaatg gattcacgaa tatcgtccaa cgctaaatgc gtattgcctt tactgaattt
     8161 atctaaaatc tccggtttcc aacggcgtgc cagctctttt aaagtactca catctaagtg
     8221 gcgataatgg aaataatcgg ctaaatcagg catatattta tacaaaaaac gtttatcttg
     8281 agcaatgctg ttgccgcaaa tcggagaagc gccttttggc acccagcgtt ttaagaagtc
     8341 taaagtttgc aactctgctg cccgttcagt caatttgctc gctttcactc gttcaatcag
     8401 cccgttagcg gtatgggttt tgacgcacca atcactcatt ttctctaata aatcatcggt
     8461 ttggtgtacc gcaagtaccg gtccttccgc taaaatattt aaatccttat cggtcacaat
     8521 ggtggcgatt tcaataatgc gttctttttc cggatctaac cctgtcattt caagatcgat
     8581 ccaaattaaa ttttgttcgt tttgagccat tttatttcct tacacttttg caatttttag
     8641 ggcatttttg accgcttgcg gcacaaattg gctgatgtca ccattgtggc gatagatttc
     8701 ccttaccatc gttgaagaaa ggtaacgcca ttctattgaa gggggaaaaa aaatagtttc
     8761 taaatcgccc gataatttgt aatttaactg agcgagttgg atttcatagt caatatcatc
     8821 tgccccacga ataccacgga ttaacgcttt ggcattctgc tgtttggcaa agtctgctaa
     8881 caatccacta aagccaatca cctcaatatt ttgccactgg gtacagcttt gtttaacaag
     8941 ctccactcgt tcttcaagac taaaaagcgg ctgcttgctt gggttttgag caacggcaac
     9001 aatcactttt ccaaataagt gagaggcttt gcaaattatg tccaaatgcc cattggtaat
     9061 gggatcaaag gtgcctgcat aaattacgtt ataactcatt ttatttctct aaataggagg
     9121 ctaataaatc caaatggcgt tttaatgccc cttgattttc ctgtaacacg cttaagccgg
     9181 attttgcaat ttcttgtccg atttggggct gcttaagcaa taattgaatg ctctcggtca
     9241 aagcctcgac tgaactttca atttcaatca cgcctttcac ctcacgcaat ttctcaaaga
     9301 tttcagggaa attataggtg taaagcccag aaaccaccgg aatattaaaa gcgattggtt
     9361 caagtggatt atgcccaccg tgtttgacca aactgcctcc tacaaaagca atgtcagaca
     9421 acccatacag taacatcatt tcccccattg tatcacctaa cattacttga gtattttggc
     9481 ttaacgtttt attctcagta cgttttacat aatttaaatc tgatttttga atcagtagct
     9541 caactaaacc aaaacgctcc ggatggcgag gcactaaaat caacactaaa ttcgggtaat
     9601 gctctaataa gagtttatga gcatctaaaa tcagcttctc ttccccttcg tgagtactgc
     9661 cggcaatcca aatcggtctc tctgttaaat tcagactagc tttagtttta atcaccgatt
     9721 gatgaagctg tggcgtaatt tctaaatcaa atttcaggtt accggtatta accatttttg
     9781 ccggtgcata accgagtgct aaatagcgat ctcggcttac ctcatcttga gccataatca
     9841 gactaatttg gttgagcata tccttaatgc taccttttac ccagccataa cgcttcgcag
     9901 aacgtggcga taagcgggca ttggcaatca caaatggaat tttacgaata tttactttac
     9961 gaattaaatt cggccaaagt tcggtttcaa ttacaatcat caatttcgga tcgataaaat
    10021 tcaaaaaccg ctcgatagca tcaggtaagt cataaggcaa ataaaaatgt gaaacactat
    10081 taccaaaagc agcttgcaca cgcccagagc ctgttggcgt taccgttgtg actatcaacg
    10141 gaagttcagg atattgcttg ctgactgcct taatcaaaag cgttgcagca atcacctcac
    10201 caaccgatgc cgcatggatt accacccctt ttgctttcgg tttttccgat tcgtcataaa
    10261 tcccataacg ctcccataaa cgtttccgat aagccggttg tttacgccct ttgtaccaca
    10321 ttaatagcag aaccacaggc tgtaatagat aacttaaaca aatatagagt aaacgtagca
    10381 tttattttcc ttataccata accctcacct cttgtgggag agagtcaaat acgccaataa
    10441 atcgttcctc tcttgttcga gaggggggaa caagcggtta tttttaccaa aaattttgca
    10501 atctatatcc tcaaagacaa aaccacggaa taaccttaat ccgtggttta tctcgcatca
    10561 ttacgcttga tgtggattgc gggtattcgt attcactaag ttacgcatta aaagtgcaaa
    10621 atccaactca atatctgccg gcacatctaa aaagacaaaa tgcccgtcac cgagtgctgc
    10681 gtccacattt tcatttttgc ggttaagcat acgctcgatt ttaaatacaa tgttgccttt
    10741 aggggtcatc atttccactg aatcgcccac taagaatttg tttttcactg ccacttccgc
    10801 caagccttct gcattgcgtt tgccggtaaa ttcgcccaca aattgttggc gttctgaaat
    10861 cgaatagcca tattcatagt tttggtattc atcgtgagta tgacgacgta agaagccctc
    10921 tgtataacca cggtgtgcaa ggctttcaag agtatccatt aaactttcgt caaacggttt
    10981 accggcagcg gcatcatcaa tcgccttacg atacacctga gccgttctcg cacagtaata
    11041 gaacgatttc gtacgcccct caattttcag tgaatgcacc cccatttgag ttaaacgctc
    11101 aacgtgttgt actgcacgca gatctttaga gttcataaaa taggtgccgt gttcatcttc
    11161 aaaggcggtc atttgctcat cagggcgttg tgattctgta taaaggaaca ctttatctgt
    11221 tgcttcgcca ttacctagag taggagccac atttttgact tcaatttccg gctcatactt
    11281 aggcacaatt tgccctacct catcaatctt accttcttcc attttatatt cccaacggca
    11341 agcattggtg caagtgcctt ggtttgggtc acgcttattg atatagcctg aaagcaagca
    11401 gcgacctgaa tatgccatac acaaagcacc gtgaacgaag atctcaagct ccatatccgg
    11461 cacttgttca cgaatttctt cgatctcttc gagtgataat tcacgggata aaatcacacg
    11521 ggttagcccc atttgacgcc agaatttcac cgttgcccag tttaccgcat tggcttgcac
    11581 cgaaagatga atatccattt cagggaaatt ttcacgcacc aacataatta agcccggatc
    11641 agacataatc aaagcgtccg gcttcatatc cactaccact tttagatctt tgatgaaggt
    11701 ttttaatttt gagttgtgtg gcgcaatgtt taccaccaca tagaattttt ttcctaaggc
    11761 gtgggcttca tcaatcgcaa cttttaaatt ggcgtggtta aattcattat tacgcacacg
    11821 caagctataa cggggctgtc ctgcataaat cgcatcggca ccataagcaa aagcgtaacg
    11881 catatttttt aaagaacctg caggcgaaag tagctcaggt ttggcatatt ttaaagtcat
    11941 attattctct tttctgataa caagtcagga cttctcgacg tgagaagcgt aagcggtata
    12001 ttttctaaga aatattgcgt gatggcaagc taatccatta caatccgatt tttatgtggg
    12061 gaacaatatg aaacttaaat accttttagc aacaacatta ctggcttttt caacatttac
    12121 cgccgctcaa agcatcaaga cggagatgat gcaaatgaat atgcaagcga ataaattgat
    12181 gcgtgccgaa tcggcagaag agtttagcga aattgcgaat gatttcatta taattaccga
    12241 gaaaaccaaa gggatttttc ccaatagcgt agaaggcgat aaagcagctc aggaagatta
    12301 ccaagccggt atgcaaaaat taattgatat ggttaatcaa gccagtgaga aagctcaagc
    12361 aggtgaattg caagaagcaa aaatgctgat ttccgagctt gaaatgatca agcgtgaata
    12421 tcacaaaaaa tacaaataat tcaacacatt aaaagggtga gctaaagctc accctttcta
    12481 ttcaaattcc agttcaaccg aattctcacc aagaagtgcc tttaattcgc taaacattgc
    12541 ctcattcggg ctgattcgcc actctactcc gcctttgagc aataccctac cttccgggct
    12601 ttggtaataa aaatgcagcg gtaaagtgcc ttctttatgt ggggtgatga tagcggtcat
    12661 ttttttaata aaatctgcag aaagttgctc tttgctaatc gctaacgcta aacttttggc
    12721 aaatcgaccc cttgcttcat caagggataa cacttctcga gccgacattt tcagtccacc
    12781 gctgaaatca tcaaactgta ccgaacctgt tgccacaata atttggtctt gttgcatcaa
    12841 atgcccgaaa ttttctaagg cttccgagaa aagggtaatg tccaatttcc ccgagcgatc
    12901 ttcaatagtc gcaatcccaa tacggcttcc tcgcttggta actgccactc gagatgccat
    12961 aacaattcct gaaaccgtgg tcatttgccc tcggaaagtc ggttgcaatt cattcagccg
    13021 attaggggca tagtgggata attctttgag gaatctacca atcgggtgtc cgcttaaata
    13081 cagcccgagc gtggcacgct ctccatctaa aatcacttgc tccgaccatt ttggcgtatt
    13141 ggcgtaagca ttttgcactt cttccggtgt ctcggtaagc acgccaaaca tatcgctctg
    13201 cccgagttcc tccattttag cgtgctgatc tgaggcttta agtgcatctt ccaaattctt
    13261 catcaaggca gctcggtgag ggcctaattt gtcaaaagca ccggacataa tcaggctttc
    13321 aaaggtgcgg cggttgattt ttttcagatc gacccgagcg gttaaatcaa atagatcttt
    13381 aaatttcccc tctttttggc gagctgccag aattgcctca atcggtcctt ccccaacgcc
    13441 tttaattgct cctagcccgt aaacaatttc gcctttttca tttacgctaa aacgatgttt
    13501 accgctgttt acatcaggcg gcacaacggt taagcccata ttcatacact catcgtagaa
    13561 cccgacaatt ttatcggtat tatccatctc tgaggtcatt accgcagcca taaattctgc
    13621 cggataatgg gcttttagcc aaagtgtttg gtaagaaacc aacgcataag ctgctgagtg
    13681 agatttatta aagccgtagc cggcaaactt ttctaccaag tcaaaaattt gaccggcaag
    13741 tttaccgtca atccctttgg cttccgcccc tttttcaaac acttcacgct gtgcagccat
    13801 ttcctccggt ttcttcttac ccattgcacg gcggagtaag tccgctccac cgagtgtata
    13861 accggcaagc tcttgtgcaa tttgcataac ctgttcttgg taaacaatga ccccataagt
    13921 cggctcgaga ataggtttta agcattcgtg ttggtattgc ggatcaggat aagaaacctc
    13981 ttctttgccg tgtttacggt caataaagtt ctgcaccata cctgattcca gcggccctgg
    14041 gcggaatagt gccaccaatg cgataatatc ttcaaaacag tccggcttca ggcgagaaat
    14101 taagtctttc attccccgtg attcaagctg gaatactgct gtagttttag ctgccagcaa
    14161 cagagcaaag gattttttat catcaagcgg aatggtttca atccgaatcg gctctttgcc
    14221 ttcacgagcc aaacgttcgt taatcatctc caacgcccat ttgatgatgg tgagcgtacg
    14281 caagcctaag aagtcaaact tcaccaatcc tgcatattcc acgtcatttt tatcaaaatg
    14341 ggtaaccggg tgtaagccct cgctatcgca ataaagcggc gaaaaatcgg taatcgcagt
    14401 tggggcgatc accacaccac cagcgtgttt acctgcatta cgagttacac cttcgagctt
    14461 acgagccata tcaataaggt ctttcacctc ttcatcggct tcatatagct caggcaattt
    14521 tggctcagcg gcaaaggctt tttcaagcgt cattcctgga tcgggcggaa tgagtttgga
    14581 gatgcgatcc acaaagctat aggggtgtcc aagcactcgc cctacatctc gaatgaccgc
    14641 tttcgccgcc atagtaccga atgtgataat ttgcgatacc gcttgtcgcc cgtaggtttc
    14701 cgcaacgtgt tcaatcacac ggtctcgccc gtccatacag aaatcaacgt caaaatcggg
    14761 cattgaaaca cgctcagggt tgagaaaacg ctcaaaaagc aaatcaaatt ccaatgggtc
    14821 aagatcggta atcttcaacg cataagccac caatgagcct gcccctgagc cacgacccgg
    14881 tcctaccgga atatcattat ctttggacca ttggataaac tccatcacga tcaaaaagta
    14941 acccgggaac cccatttggt tgatcacatc taattcaact tgcaatcttt cgtcataaac
    15001 cgaccgcttg ttcgcccttt cctcaggatc agggaataga aactctaaac gctcttctaa
    15061 gccttctttc gattttttta ccaagaaatc ttcggtggaa agctcaccgg tcgggaaatt
    15121 tggcaggaaa tactcgccca aacgcacggt aaccgaacag cgtttcgcaa tttcaaccgt
    15181 gtttgccaat gcactcggta aatcggcaaa cagcttgcac atttcctctt cagtacggaa
    15241 atattgtttc ggtgagtatt ttttcgggcg tttcgggtcg tctagggtgt agctgtcgtg
    15301 aatggcaacc cgaatttcgt gtgcgtcaaa gtcatcttct ttcaagaaga caacatcatt
    15361 taccgccact aacggaattg catttttttg tgaaaattcg accgcttgtt tgatatagcg
    15421 ttcttcgtct gcccgtccgg tacggcaaac ggaaaggtaa aaatgattcg ggtaatattg
    15481 ctgataaaaa gctaattttt cttcagcttc ttgtcggttt tctttgagta aggctttacc
    15541 cacatcgccc attcgcccgc cggaaagaat gatgatgccc tcattcagct ctgctaacca
    15601 ttcacgttcc accatcggaa attccacata gccttgctga taggctttgg acaaaatttg
    15661 ggtaatatta tggtagcctt tgttgttctt cgccagtaat gtcagctcaa aataatcttc
    15721 attgccgctc tcggttctaa cataaacatc tgccccgata atcggcttaa tccctgctcc
    15781 caatgcttcg ccatagaatt tcaccaaccc gcaaaagttt ataaaatccg ttaagcccat
    15841 tgccaccata ttattagcgg cagcggtttt aactaaggat tttaccttag ctaaaccatt
    15901 aatcattgag aaatcactgt gaacttttaa atgaacgaaa cgacaatctg acataggaat
    15961 ataatctaag taaagaagtt tcccctcttt agtaaagact gtcaggttct aaaaagtgaa
    16021 gccttctgtg aataatcccg ttgggcgaat gttattcgcc cctacaaacg agcattattc
    16081 ttgctcagag ttacatttaa aatccgaatg taggggcgaa ttacattcgc cctttcagaa
    16141 cctaacaacc ctgctttatc taaagaaggg agttaaaatt aatcacgttt tttgcttaac
    16201 agccaccaaa cgatgatcaa acagagtagg cttggaatta atgctccaac cggtataggt
    16261 aaccaagtgg caattaagct taaattgcca aacacaatat ttgccacata gaacataaag
    16321 ccggcaacaa taccaatcac aattttcgcc cccatcgtac tactacgaag cggcccgaaa
    16381 ataaaggaga tcgcaagtag catcatcacc gccattgaga ttggttggaa tacttttcgc
    16441 cagaaggtga tctcaaaacg tttcggatct tgtccggtat cttttaagaa gccgacataa
    16501 tctgccaagc ctgaaatcga gagcgactcc ggtttgagtg aaacaatgcc taatttgcta
    16561 ggggttaaac tggtttgcca aacttgatct ggctgtttag cttgctggat ctgattttct
    16621 cccacagtcg atttttccac tttagatagc acccagcctt tttcatcact gtaattcgca
    16681 gttcgggctt ttaaaacaga ttgcaattca cgtttgtttt gtccttcgcc gaattgataa
    16741 atcagtacgt tatctaagct tttttcattt ttaatatgat taatatagat aaatgaattg
    16801 ccatctttcg cccaaaaccc gtcttttgtg gcaagcattg agccgccact ttgggcaatc
    16861 gaacgcatat tacgggcaaa ctgctcggtt tgtggcacgc cccattcgcc gataatcatc
    16921 gtaaaaatga ctaacggtaa ggcagttttc atcacggcta aaccaatgcg gaaacgggaa
    16981 aaaccggagg cttgcatcac caccaattca ctaaggctcg ctaggttgcc taaaccgagt
    17041 aatgagccaa gtaatgccgc aatcgggaaa aaggtttcga tatctcgtgg aatagttaaa
    17101 aaagtatagt atgccgcttt taagccatcg taagatccac gtcctacatc acgaaactct
    17161 tccacaaatt taataatcgc cccaagccca atcaacataa acagggttaa aaaaatcgcg
    17221 gataaaatcg ttttgccgat gtagcgttct aaaatattca ttgagaaaaa ttgcattatg
    17281 ccaccgcctt attggttaat ttttttgcac caaattgata gcgtaactta gacataaatt
    17341 tactatccca cgcatttaaa ataacaccta aaactaaaaa ggcaactgat accaacggca
    17401 ttaacacttc cgcattcaac ttgcctgatg cccctgacga ttttagcgag ctttgtagta
    17461 agaaatagat taaataaagc aataacgccg gcagtagttt agcaaaacgc ccttgacgag
    17521 ggtttacact actcattggc accgctaata atgccattaa cggtacagct aaaatcaaag
    17581 caagccgcca atgaagctcg gcttttgctt ccggcgtttt ggtttccatt aatttcttga
    17641 aatcggctcg ttgcactaat ttctcatcag attcaacgtc ttgatagcct aaataagcag
    17701 aataattctc aaaatgtgaa atacggaaat cggcactttg tggtgtacct tcaaaacgct
    17761 tgctctctgc caaggttaag acttgatccc catttggcaa accttgcaac gtgccggatt
    17821 cagcaacaac cactgaaggt ttgtttttct tctgctgttc cggctggaaa acgtagatat
    17881 catttagact gttattttca attttatcaa taaataacac atagccaccg gcagacataa
    17941 attgtccggc cgaaagagcc gcaaaacgtg ggttggcttt cgcttccgct aacatttcac
    18001 tctgtttatt aattgcccaa ggcgtaagcc aaaacacgtt ataaaccgct aatgcagtgg
    18061 taaaaatcga gagaaacatc gccactttaa ccaatagccc ttgcccgata ccacaagccc
    18121 gcattacggt aatttcactt tcggcataaa accgcccgag ggttaataac aaggcgataa
    18181 acagtgagag tggcagcatt aattgtgcca ttgtcggcat acctaagcct aataagctca
    18241 ccactaaatc tgccggtact ttaccactca cagcagaact tagaacacgc actaattgct
    18301 gacaaaaaaa gatcagtaat aagataaaca atatcgccaa ttggctttta aatatctctt
    18361 tggttaaata tcggcttaag atcacgtttt ttctctataa catttacaaa tctgaatacc
    18421 gctaaacaca agcggtctat ttttgttaat tttttgcaaa gagcgcaaaa taacttattc
    18481 gaatcgctaa taataccttt ttcatacaaa aaaatcacct atagaaaata tcacgcacgg
    18541 caaaaccgtg cgtaagtatt attgtttttt cgattttttc gatgatgttg ctttatgagc
    18601 ttcaagaatt aatttcttac cttggctatc ttcaatttca atcttaggtg gtgcttccgt
    18661 attttgatga aggatagcta taatattttc accttgtgca atatcttcaa agtttccttt
    18721 cacacgtaac ttagccgcat caacactagt gtcaaatact aagaatgtac ctaatgcggt
    18781 aactctaata tttttaagcc ctatattgtc cttcgttacc gttttatatt gaggtgcttt
    18841 tttccctttt gcagccataa tcttctcttg attagtcgca atattaagaa tattttgaac
    18901 tttagtcgct tctgctgtag attgtgcctg ttctgttaaa tttggttctc catcaaaaat
    18961 aacatcccca acaaattgca cctctttagg ctcattttgc aataatggtg caacgtcttt
    19021 agctgaaaca accaaaaata accgttcacc tattttttca aaacggacat caatattggt
    19081 taattgtgat tcaatttgtt cattaattgt cttgatcgca gtttctgtct cattttgaag
    19141 cgatatttta cgtgtaatat caacatcata agcaataaaa ccaactttta aacgttgtga
    19201 taactgctga agtaaattgc ccacatttcc atcataagaa agacgtaact ttgcctgctc
    19261 atagcgacta atttgagcat catcattagc gaaaatagtg ttttgagtcc ctaagccgat
    19321 tagaccaaca acaaacgttt taccccaaaa gcgcat
//
LOCUS       DANLXS010000025        19212 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.025, whole genome shotgun sequence.
ACCESSION   DANLXS010000025 DANLXS010000000
VERSION     DANLXS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19212
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.025"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            276..488
                     /locus_tag="PXD71_000697"
     CDS             276..488
                     /locus_tag="PXD71_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL6290851.1"
                     /translation="MSAITGTVKWFNATKGFGFITPDQGGKDVFVHFSAIEANGGYRT
                     LEDGAKVQFEIQDSERGASAVNVKTI"
     gene            complement(548..1702)
                     /locus_tag="PXD71_000698"
     CDS             complement(548..1702)
                     /locus_tag="PXD71_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDL6290852.1"
                     /translation="MMKVSPFQWAAFNYFGFFCAYGVLLPFLPVWLKHHGYSTEMIGL
                     FAAVGYLFRFAGSMLASQRVKTVSQLIPTARSLTWLNIAAAAALLFSGDFIWLLFPVL
                     MLFQMFNSGAMPIADSIASIWQHQIGLDYGRARLFGSMAFVVGSLSAGYLIGLLGENS
                     VVWIIIGFLVFLGIGQLATPKVGFEEQGNKANTSSVTYRQLLKEPETLKMLIAVSLIS
                     ASHAAYYTYSTIHWSNASISTAMTSLFWGLAVVAEILFFLVSKRLFKSWKISHLMILA
                     AVFAVIRWAIMGSTTEIVLIIVMQMLHSITFGAAHIAMIRYISMQSAERVTKLQGLYF
                     GLASCAFMAVFTFIAGMVYQHSPSLTFWLMAIFTLPAIFIVPKRFEVQIN"
     gene            complement(1699..2517)
                     /locus_tag="PXD71_000699"
     CDS             complement(1699..2517)
                     /locus_tag="PXD71_000699"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL6290853.1"
                     /translation="MNEKKLAFIGTGNMAYAIIQGLLKSGYSAEQIIACNKSNLARRE
                     ELQAVGIATNFANREAVEQADVIVLAVKPQMMAQVCAEFADVDFSNKWVISVAAGISV
                     QRLETLLPSAKNIIRTMPNTPSLISEGMTGLFAKKSVNPTACQFAEQLMSAVGKCYWV
                     ETEEQINHIIAITGSSPAYFFRFMEAMQQSAVQMGFSEQDARTLVQSVALGAAKLVEA
                     NPTTPLSTLRENVTSKGGTTAKALEVFEQHDLAKTVDDAMWAAIHRAREMEQSL"
     gene            2594..3502
                     /gene="rdgC"
                     /locus_tag="PXD71_000700"
     CDS             2594..3502
                     /gene="rdgC"
                     /locus_tag="PXD71_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDL6290854.1"
                     /translation="MVWFKNVMIYKLTGELALESNSLNELQQETRFTPCSQHDMSKFG
                     WATPLTGSDLLHFSQANQFLLISHKEDKLLPANVVKAETEQRIAELEEKEARKLKKTE
                     KQAVKDQVVSALLPRAFSKHQFTALWLDLDNQLVYVDAASSKRAEDTLALLRKTLGSL
                     PVVPISFTLPPAEVMTNWIAKGHTPSWLNLLEEAELKSFDTDSIIRCKRQDLETEEIA
                     RHLQAGKFITKLAIEWEGHFNCVLNEDATLSRLKFADEVREKNDDILKEDVAQRFDAD
                     FLLMTEELKLFTEKMIAEFGGIKSRI"
     gene            3523..4128
                     /locus_tag="PXD71_000701"
     CDS             3523..4128
                     /locus_tag="PXD71_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HDL6290855.1"
                     /translation="MLDQTIIDQYDALIFDMDGTLIDTMPSHAKAWEKVGEVLGYPIN
                     PKPMYELSGSTTFVIAREIMQRSNIPEHYFEQVVQLKREFGIEMVLANATLLPAFEII
                     KANVGKKPMAIGTGSHRAMVELLDQKFNLRQYVSVIVDSDDVSNHKPAPETFLKCAEK
                     LGIAPHRCLVFEDADLGVQAALNGNMNVFDVRTNTITHSTR"
     gene            4144..4935
                     /locus_tag="PXD71_000702"
     CDS             4144..4935
                     /locus_tag="PXD71_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL6290856.1"
                     /translation="MKTEWIKHLIQQLTTLSKAIENYFTSGSFGVKFYPFLMHPCFRK
                     SCAFLIFIIAMVSSFSGLFYLVAEHITILFSERSLTTYFHHSTLELMIPVGTMIDEKI
                     SELSPLSVVMRSMFVLQAVVFFFIYAIGITHIAHNKLRVIGLLLSLCFAIGCSLVAGI
                     QPSSRGEFGIYNLGASITFLIGNLTLIIAGFDLYHPSMQFFKRFSIGAGVIGAICILI
                     TIFLPTLFSPLIERAGIYTIMLWEILAGFAIIKRLLKLPSFRQQG"
     gene            4939..6081
                     /gene="argE"
                     /locus_tag="PXD71_000703"
     CDS             4939..6081
                     /gene="argE"
                     /locus_tag="PXD71_000703"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDL6290857.1"
                     /translation="MKQIPFIQRYAQLIELPTISSLVAEEDLSNRRLIELLATWLADF
                     GFKTEILAVEGSRNKYNLLATYGEGEGGLLLAGHTDTVPFDEGKWRFNPFQLTEKAGK
                     LYGLGTADMKGFFAFVVEVVSQLDLTQIKKPIRILATADEETTMLGARTFAQHSHIRP
                     DCAIIGEPTSLKPIRAHKGHIGESVRITGRSGHSSDPAKGINAIELMHQSIGYLMKMR
                     DELREKYHNPLFQVTHPTMNFGNIHGGDAINRICACCELQFDMRPLPNMAVQDLYAMV
                     NEHLKPMLEQYGDLIEIRHLHDGIPGYECEHSAQIVQVVEKLLGEKCDAVNYCTEAPF
                     IQQLCPTLVLGPGSIEQAHQPDEFLEMKYIEPTKELLTKLIYHFCG"
     gene            complement(6228..6515)
                     /locus_tag="PXD71_000704"
     CDS             complement(6228..6515)
                     /locus_tag="PXD71_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6290858.1"
                     /translation="MPKSWELSLTARSNVIEILQSVEDYTGHWLSAVSLWEQLQNKLD
                     LIAFMPTIGRRREDGTLETFCRNYRIVYEIHLEKVYIITIIHSRRLYPRPS"
     gene            complement(6508..6699)
                     /locus_tag="PXD71_000705"
     CDS             complement(6508..6699)
                     /locus_tag="PXD71_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290859.1"
                     /translation="MLFKEQGYDEFLAEQIKAGQEAIARGETFTKEQINNELNHLFAD
                     MQKRQQLRENENLFEVNYA"
     gene            complement(6989..7579)
                     /gene="yraP"
                     /locus_tag="PXD71_000706"
     CDS             complement(6989..7579)
                     /gene="yraP"
                     /locus_tag="PXD71_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDL6290860.1"
                     /translation="MNKLANKLLKGSLLVLGFISLQGCITTAVVTSAAVATKVATDPR
                     TTGTQVDDEILEEKVAYNINKDEQIKQEARINVVAYNGKVLLIGQAPSMDVVENAKNL
                     AAGAEGVTEIYNEIRQGEKIGFGQITQDSWITTQVKSKLLVNSEVKATEVKVVTENGE
                     VFLMGKVSQNQADAAAEAARNVGGVTKVVKVFSYAQ"
     gene            complement(7589..8173)
                     /locus_tag="PXD71_000707"
     CDS             complement(7589..8173)
                     /locus_tag="PXD71_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL6290861.1"
                     /translation="MLNKIQDRFTESIQTQISASELLPAKLSEAANRIVACLLRGDKV
                     IVCGHGRSYGNAELLVSHLLHRYELARPSFAAVQLQLSGVLAGVVAQDNELEAVYKKQ
                     LQAVAKEGDLFIVFSPVGNEECILQAIHTAKNQHLDLDIIALTGSNNDHTLGLLDESD
                     IEISAPSTNQLRVIESHHFYVNLLCELIDHLLFS"
     gene            complement(8179..8550)
                     /locus_tag="PXD71_000708"
     CDS             complement(8179..8550)
                     /locus_tag="PXD71_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDL6290862.1"
                     /translation="MELFNKLINKREQGSFYEQKARVFLEQQGLRFVAANQAFKGGEL
                     DPIMQDGQTVVFVEVRQRKSNRFGSAVESIDYRKQQKWQNAANMWLLKQNQSLETANC
                     RFDVVAFEGNQTPVWIPNFLG"
     gene            complement(8525..10246)
                     /locus_tag="PXD71_000709"
     CDS             complement(8525..10246)
                     /locus_tag="PXD71_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HDL6290863.1"
                     /translation="MATILNHTMKKALVPTAIALFISACTSVNPVTESIKNEAYSSSE
                     FYINKADQTKEAEDKISYQLLAVRKLIDENKEYEAQNTFSEILTAEMNEVQKLEYALV
                     SAQLAALQGKNEQATSQLKAIQDPLLSSAQRLRYIQTQARIAANQKDVIGIVRARSQL
                     NNFYKMNRERQENNDIIWQTLRDANRGMLEKTVPEAGEMELAGWLALINIYNQNVTTP
                     AQMPQAINNWKAQYPSHSAVAVMPTELQGVSNFQQTQLNSVALLLPLSGDAKILGDII
                     KRGFNDAKADDSTAVQTFDTDSSDVNSLISQAKQQGANVIVGPLLKSRVDEMLLSPEI
                     QNVNILALNATENVRNIAQVCYYGLSPESEAQSGAEKIYQDGNSVAIVAAPQDDYGNR
                     SAEAFAKRWRQLTNSDADVRYYNQPLDAVAAIQNAGVSKAALYILGNAEQVLEIKQGI
                     DSSTLKDRLAIYTSSRSNSPNNGIDFYTSMEGVKFSEVPLLADQSSSEYKKAENLANS
                     DFSMMRLYAMGADAWSLATKFNEFRHIPGYKISGLTGELKAGQHCNIERSLSWMQYRN
                     GAIQQAY"
     gene            10320..11192
                     /gene="rsmI"
                     /locus_tag="PXD71_000710"
     CDS             10320..11192
                     /gene="rsmI"
                     /locus_tag="PXD71_000710"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL6290864.1"
                     /translation="MKNRQNSSGINPNYGILYIVATPIGNLGDITQRALDTLSSVDLV
                     AAEDTRHSGLLLSHYGIKKPFFALHDHNEQQKAVVLVEKLAKGENIALISDAGTPLIS
                     DPGFHLVRHCRQAGVKVVPIPGACAAVTALCASGIASDRFCFEGFLPAKTKSRCDKLT
                     ELENEPRTLIFYESTHRILDTLADMQTVFGEDRYVVMAREITKTWETIYGDQLGNLIE
                     WLNEDSNRIKGEIVLVVEGKPQSSEEAFSSQAIKLLTLLCQELPLKKAAAIVAETFGY
                     KKNALYQYGLEHFE"
     gene            complement(11437..12603)
                     /locus_tag="PXD71_000711"
     CDS             complement(11437..12603)
                     /locus_tag="PXD71_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6290865.1"
                     /translation="MTNLLLAIIYLSFISLGLPDAILGSAWPSMHQQLAVPLSYAGVV
                     SMIMSIGTITSSLMSDKLTYHLGTGKVMVISVALTALALWGFSISDAFWILCLWAIPY
                     GLGAGAVDAALNNYVAVHYASRHMSWLHCMWGVGASIGPHILGYALLQGRGWEAGYFT
                     ISMLQIGLTLMLFCSLPFWKLRKQITAEINHSAKPMSLKETLKIPGVKYGILAFFGYC
                     GVEQTAGLWASSYFVMVKDVGLEQAAAFGSIFFIGITVGRGVSGFLTLKFSNRQMIKL
                     GSALILVGIAILCLPLGKYVTLASLIIIGLGCAPIFPCLIHSTPAFFGEDKSQSVIGV
                     QMASAYIGVLLLPPIFGFIANAISIQLLPLYLLLMFAIMAYAYRNLVRETSPSP"
     gene            complement(12755..14269)
                     /gene="putP"
                     /locus_tag="PXD71_000712"
     CDS             complement(12755..14269)
                     /gene="putP"
                     /locus_tag="PXD71_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDL6290866.1"
                     /translation="MFGLDPTTIMFVVYILGMIAIGFIAYRVTNNLSDYILGGRRLGS
                     FVTALSAGASDMSGWLLMGLPGAVYAGGLIEGWIAIGLTIGAYLNWKLVAGRLRSHTE
                     HSGDALTLPEYFHNRFGDKTRLIKILSAVIFLLFFAIYCASGVVAGARVFENLFGLPY
                     HQAIWYGAVATILYTFIGGFLAVSWTDTIQASLMVFALILTPFFVMYNLGGFDEMQAL
                     IDRAGEMAQKDYNDIFTGTSVLGIISLMAWGLGYFGQPHIVVRFMAAESVKSLENARR
                     ISMTWMIICLLGAIGIGYFGMAYFFGNPEQVGNISKNPEQIFIELAKLLFNPWVAGIL
                     LSAILAAVMSTLSAQLLICSSAITEDLYKGILRPQASDKELVWLSRLMVLAVAALAIY
                     IAQDPDSKVFGLVRNAWAGFGSAFGPVVLLSLFWKRMNGFGAIAGMLTGALIVHFWGD
                     ITLKLALPEIYSMIPGFIAATLMIIVVSLITPMPSKAVLDTFDRANKAYLAEVK"
     gene            complement(14363..15151)
                     /locus_tag="PXD71_000713"
     CDS             complement(14363..15151)
                     /locus_tag="PXD71_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL6290867.1"
                     /translation="MNAKKEKESSGNQSLIRGLTLLELLAKFPNGCPLAHLAELSGLN
                     KSTVHRLLQGLQQEGYVRPAPTAGSYRLTTKCLAIGQRALSSINILHIAAPHLEALNL
                     TLGETVNFAMRENDHAILINKLEATTGMMRTRAYIGQQMQLYCSGMGKLFLAYGSADY
                     IPQYWQTKSDIIQKLTVNTITTIDGMQTELETIRRQGFAMDNEENELGVSCIACPIFD
                     HQQKVHYAISVSLSTARLNQLGRENLLPAIQETAEKISAELGGR"
     gene            complement(15148..15726)
                     /locus_tag="PXD71_000714"
     CDS             complement(15148..15726)
                     /locus_tag="PXD71_000714"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL6290868.1"
                     /translation="MNQSSLTDLRSQLRQSMRLKRQSLTAGQQTQAAESIIPQALSLI
                     ESYQVSHIAFYLPFNGEISPIPLMEQLLQQGKKLYLPLLHPFTSGQLLFVNYNCKTIL
                     KFNRLGIQEPILDVRNILPLQELEMIFTPLVACDKAGNRLGMGGGFYDRTLAQAPHLI
                     SVGLAHECQQVEQLPIESWDMPLDHIILGAAK"
     gene            complement(15728..15919)
                     /gene="ssrS"
                     /locus_tag="PXD71_000715"
     ncRNA           complement(15728..15919)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PXD71_000715"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16024..16314)
                     /locus_tag="PXD71_000716"
     CDS             complement(16024..16314)
                     /locus_tag="PXD71_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL6290869.1"
                     /translation="MSANNIELQIFGQVLRLQCPVEQQEHLLASAQRLEERVVVLKEQ
                     SGIIQLEKVLAIVALNLNYELEQAQRQNAENKNVLVACIEQLDNSLGKFQSN"
     gene            16393..16950
                     /locus_tag="PXD71_000717"
     CDS             16393..16950
                     /locus_tag="PXD71_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HDL6290870.1"
                     /translation="MAITDSQQFKQLVSQLQIDDTPAEFHGFLCGLIAGGIQDESWKT
                     LTYQFTNDGHAFSIEPLNTLTDFYQQLSESFSEANTLFSLWLPQNEEDGFALADGISE
                     WTNSFLLGLGVAQPKLQQETDEVGEAIDDLDEIAKLGYSDDDNNEELLEAGEEVLEYL
                     RVLALFLHSHFALGVPKTEEKPPLH"
     gene            17048..18112
                     /gene="hemE"
                     /locus_tag="PXD71_000718"
     CDS             17048..18112
                     /gene="hemE"
                     /locus_tag="PXD71_000718"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDL6290871.1"
                     /translation="MSELKNDRYLKALLREPVDMTPVWMMRQAGRYLPEYKATRAEAG
                     DFMSLCRNADLACEVTLQPLRRYELDAAILFSDILTVPDAMGLGLSFGAGEGPKFARP
                     VASLNDVNQLPIPDPEDELQYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTKIKKMLYAEPHILHKLLDKLADSVILYLNAQIKAGAQAVMVFDTWGGVLAHED
                     YKAFSLHYMHKIVDGLIRENEGRKVPVTLFTKGGGLWLEAIAETGCDAVGLDWTVDIA
                     DARRRIGDKVALQGNMDPSVLYASPERIEQEVKNILAGFGNGSGHVFNLGHGIHLDVP
                     VESPKVFVDAIHNYSKQYHK"
     gene            18259..18849
                     /locus_tag="PXD71_000719"
     CDS             18259..18849
                     /locus_tag="PXD71_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HDL6290872.1"
                     /translation="MRNPIHKRMERFEPWQNTTFMACLCERMYPNYQLFCEVTEQPEK
                     AKVFQNILNLVWEYLTIKGAKINFDNQLEKLEEIIPDVNEYAFFGVLPAQEACEALSE
                     LLHSIIAGSTLEQAIRISQISLGTVASYLEMQQDKELNEQELKNSEEIQEELDVQWQI
                     YRLLNECEKRDLDLIFELKDEIRSSGISNIGLNINQ"
     gene            18985..>19212
                     /locus_tag="PXD71_000720"
     CDS             18985..>19212
                     /locus_tag="PXD71_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL6290873.1"
                     /translation="MNKTELIDAIAAGANLTKKDAKAALEATLDAISASLKAGDAVQL
                     IGFGTFKVNERKARTGRNPKTGEEIKIAAAKV"
BASE COUNT         5695 a         4253 c         3942 g         5322 t
ORIGIN      
        1 ggatactata tttgatttat aaggcttcgt ccatataaat tccacattac gtttacaaaa
       61 gagatagact cttttttcat ttgaatgttc ataaaagtat aaaaatgatt tgcttaaaaa
      121 atgaataagt ggtgtaatgg ctcaagctgt tcgtgcgtta attaaagttg aatcttgtta
      181 gttattttcc tcaaggcaag tgattgatag atacgtctgt aatatcccgt ttagctatct
      241 tttatcactt tttgtattta tataaggaaa attttatgtc agcaattacc ggcacagtaa
      301 aatggttcaa cgccaccaaa ggttttggtt ttatcacccc tgatcaaggt ggcaaagatg
      361 tattcgtaca cttctctgct atcgaagcaa acggcggcta ccgcacatta gaagacggtg
      421 ctaaagtaca atttgaaatc caagattcag agcgtggagc ttcagccgta aacgtgaaaa
      481 caatctaatt tcagcaaatt caatgaattt cgataaaaat gccgacaact tgtcggcatt
      541 tttgctatca attaatctgt acttcaaacc ttttcggcac aataaaaatg gcaggtaggg
      601 taaaaattgc catcaaccaa aatgtcaggc ttggggaatg ttgatagacc attccggcaa
      661 taaaggtaaa tactgccata aaagcacagc ttgccaagcc aaaatagaga ccttgtaatt
      721 tcgtcacacg ctctgccgat tgcatcgaaa tatagcgaat catcgcaata tgagccgccc
      781 cgaaggtaat agaatgtagc atttgcatta caataatcag cacgatctct gtagtactac
      841 ccattattgc ccaacgaatc actgcaaaaa cggcagctaa aatcattaaa tggctgattt
      901 tccacgattt aaacaagcgt ttagagacca aaaagaacaa aatttccgcc accaccgcca
      961 agccccaaaa caggctagtc attgcggttg aaatactggc atttgaccaa tgaatggtgc
     1021 tgtaagtgta ataagcggcg tgcgaggctg aaattaacga caccgcgatc aacattttta
     1081 aggtttccgg ctctttcaat aattggcgat aagttacgct tgaagtattg gctttattac
     1141 cttgttcttc aaacccgact ttgggcgtag caagctgccc tattcctaaa aaaaccaaga
     1201 aaccgatgat aatccacacc accgaatttt caccaagcaa gccgataaga tagccggcag
     1261 aaagtgagcc taccacaaac gccattgagc cgaataacct tgctctgccg taatccagcc
     1321 cgatctgatg ttgccaaatt gaggcaatgc tatcggctat cggcattgcc cccgaattga
     1381 acatttgaaa caacattaac accggaaaaa gtagccaaat gaaatcccct gaaaacagca
     1441 acgccgccgc agccgcaata ttcagccaag ttaaactgcg ggcggtggga atgagttggc
     1501 tcacggtttt tacccgttgt gaggcaagca tactgccggc aaagcggaac aaatagccaa
     1561 cggcggcaaa taagccgatc atttcagtcg aatagccgtg atgtttaagc cacactggca
     1621 aaaacggcaa caacacgcca taggcacaaa aaaagccaaa gtaattaaat gccgcccatt
     1681 ggaacgggga aactttcatc ataacgattg ctccatttct ctggctcgat gaattgccgc
     1741 ccacattgca tcatcaacgg ttttagcaag atcgtgctgc tcaaacacct caagggcttt
     1801 cgccgttgtg cctccctttg aggtcacatt ttcccgcaag gtcgaaagtg gcgtagtcgg
     1861 gttcgcttcc accaatttcg ccgcccccaa tgcgacagat tgtacaagcg ttcttgcgtc
     1921 ctgctcggaa aagcccattt gcaccgcact ttgctgcatt gcctccataa aacggaagaa
     1981 atacgccggg cttgagccgg ttatcgcaat aatgtggttg atttgctcct cggtttccac
     2041 ccagtaacat ttaccaacgg ctgacatcaa ctgttcggca aattggcaag cggtcggatt
     2101 taccgatttt tttgcaaata atccggtcat tccttcgctg attaaagacg gggtgtttgg
     2161 catcgtgcga atgatatttt tcgcactcgg caacagcgtt tccaagcgtt gcacagaaat
     2221 acctgctgct actgaaatca cccatttgtt ggaaaaatca acatcggcaa actcggcaca
     2281 cacttgtgcc atcatctgtg gttttaccgc cagcacgatc acgtcagcct gctctaccgc
     2341 ctcacgattt gcaaaatttg tcgcaattcc gaccgcttgt aactcctctc ggcgagccaa
     2401 attactttta ttgcacgcaa tgatttgttc ggcagaataa ccgcttttta ataagccttg
     2461 aataatcgca tacgccatat ttccggtgcc gataaaggca agttttttct cgttcattcg
     2521 caaattctct actgtgttgg gttgaggtct gttaaaataa cactattcta cacccgataa
     2581 aggatatgca aaaatggttt ggttcaaaaa tgtgatgatc tataaattaa ccggcgagct
     2641 ggcgttggaa agcaatagcc ttaacgagtt acagcaagaa acccgcttta ccccttgcag
     2701 ccagcacgat atgagcaaat tcggctgggc tacgccactt accggctcgg atttactgca
     2761 tttctcacaa gcgaaccaat ttttgctgat ttcacacaaa gaagataaat tattacctgc
     2821 caatgtggtg aaagccgaaa ccgagcaacg cattgcagaa ctggaagaaa aagaggctcg
     2881 taagctgaaa aaaaccgaaa aacaagccgt gaaagatcag gtcgtttctg cgttattgcc
     2941 tcgagcattc agcaaacatc aattcaccgc tctttggttg gatttagata atcagttggt
     3001 ttatgtggat gcagcctcga gcaaacgggc ggaagatacg ctggcgttac tgcgaaaaac
     3061 acttggctcg ttgccggttg tgccgatttc ctttacccta ccgccggcag aggtaatgac
     3121 gaattggatc gccaaagggc atacgccaag ttggttgaat ttgttggaag aggcagaact
     3181 gaaatctttc gataccgaca gcattattcg ctgtaaacgc caagatttag agacggaaga
     3241 aatcgcccgg cacttgcaag cgggcaaatt tatcacaaaa cttgcgattg aatgggaagg
     3301 gcatttcaat tgcgtgttaa atgaagacgc cacgctttca cgcctgaagt ttgccgatga
     3361 agtgcgagag aaaaatgacg atattctcaa agaagatgtg gctcaacgct ttgatgccga
     3421 tttcttatta atgaccgaag agctgaaact tttcaccgaa aaaatgattg ccgaatttgg
     3481 cggaattaaa tccagaattt agaaaaaaat ttaaggaaag caatgttaga tcaaaccatt
     3541 atcgaccaat atgacgcctt gatttttgat atggacggca cattaattga cacgatgcca
     3601 agccacgcca aggcgtggga aaaggtgggc gaagtgttag gctacccaat caatccaaag
     3661 ccaatgtacg aactgagtgg ctcaaccact tttgtgattg cccgagaaat aatgcaacgc
     3721 tccaatatcc ccgagcatta ttttgagcaa gtggtacaac taaaacggga atttgggatt
     3781 gaaatggtgt tagccaatgc cactctgttg cctgcctttg agattattaa agctaacgtt
     3841 ggcaaaaaac caatggcaat cggcacaggc tcacaccgtg caatggtgga attattagac
     3901 caaaaattta acttacgtca atatgtttcg gttattgtag attctgatga tgtaagcaac
     3961 cataagccag cccctgaaac ctttttaaaa tgtgcggaaa aacttggcat tgccccacac
     4021 cgctgtttgg tgtttgaaga tgccgattta ggcgtgcaag ccgccctcaa cggcaatatg
     4081 aacgtgtttg atgtacgtac gaatacgata actcactcaa cccgttaaaa atgataggtt
     4141 tatatgaaaa cagaatggat taaacattta attcaacaat taaccacgct ttccaaagcc
     4201 atcgaaaact actttaccag cggttcattt ggggtgaaat tctacccttt tctaatgcac
     4261 ccttgttttc gtaaaagctg tgcctttttg atttttatta ttgctatggt ttcaagtttt
     4321 agcgggctat tttatttggt ggcggaacac atcacgattt tatttagcga aagatcgctc
     4381 accacttact tccaccatag cacccttgaa ttgatgattc cagtcggcac aatgatagat
     4441 gaaaaaatca gcgaattatc accactttcg gtggtaatgc gttcaatgtt tgtgctacaa
     4501 gcagtcgttt ttttctttat ttatgcaatc ggcatcaccc acattgctca caacaaattg
     4561 cgggtcatcg gcttattgct ttcgctatgc tttgcgattg gctgctcact agttgccggt
     4621 attcagccaa gcagtcgtgg cgaattcggc atttacaatt taggggcaag cattacgttt
     4681 ttaatcggca atttaacgct gatcatcgca ggcttcgatc tctaccaccc ttcaatgcaa
     4741 ttttttaaac gctttagcat tggtgcgggc gttatcgggg caatctgtat tttaatcacg
     4801 attttcctac caaccctgtt tagcccgtta attgaacgag caggtattta taccattatg
     4861 ctgtgggaaa ttttagccgg ttttgccatc attaaacgcc tgttgaaatt gccctcattc
     4921 agacaacaag gataaccaat gaaacagatt ccctttattc aacgctatgc tcaactgatt
     4981 gagctaccga ccatttccag ccttgttgcc gaagaagatt tatcgaaccg ccgtttgatt
     5041 gaattgctcg ccacttggct ggcagatttc ggctttaaaa ccgagatttt agcggtagaa
     5101 ggcagccgta ataaatacaa tttacttgcg acctatggcg agggtgaggg cggcttatta
     5161 ttggcagggc ataccgacac cgtgccgttt gatgaaggaa aatggcgctt caatccattc
     5221 caattaaccg aaaaagcagg caaactctac ggcttaggca cggcagatat gaaaggcttt
     5281 ttcgcctttg tggtggaggt tgtgagccaa ctcgacctaa cccaaatcaa aaagccgatt
     5341 cgcattcttg ccaccgctga tgaagaaacc acaatgctgg gggcaagaac ctttgctcag
     5401 cacagccaca ttcgccccga ttgtgcgatt attggcgagc cgacctcgct caaaccgatt
     5461 cgagcccaca aagggcatat tggcgaatcg gttcgcatta ccggcagaag cggacactcc
     5521 agcgaccccg ccaaaggcat taatgcgatt gaattaatgc accaatctat cggttattta
     5581 atgaaaatgc gtgatgaact gcgagagaaa taccacaatc cgctgttcca agtcacccac
     5641 ccgacaatga acttcggcaa tattcacggc ggcgatgcca ttaaccgcat ttgtgcctgt
     5701 tgcgaattgc aatttgatat gcgaccacta ccgaatatgg cggtgcaaga tttgtatgca
     5761 atggtaaacg agcatttaaa accgatgctg gaacaatatg gcgatttaat tgaaatccgc
     5821 catttacacg atggaattcc gggctacgaa tgcgaacact cggcacaaat cgtgcaagtg
     5881 gttgaaaagc tactgggcga aaaatgcgat gccgtcaatt actgcaccga agccccattc
     5941 attcaacaac tctgcccaac gctggtacta ggccccggct ccattgaaca agcccaccag
     6001 cccgatgagt ttttggaaat gaaatatatc gaaccgacaa aagagttatt gactaaattg
     6061 atttatcatt tttgtggata aatcaacaga aacaagcgct cattttcgcc atactttttg
     6121 caaaagaatc ggcaaaaatg accgcttgat atttagtatt gagtgaccta taaacttcca
     6181 tacttcatca ttgatttaag gagattctgt tagataggta ttaatctcta gctaggtctt
     6241 ggatacaaac ggcgagaatg aattatagta ataatatata ctttttctag gtgtatctca
     6301 tagacaatac gataattgcg gcagaatgtt tctaatgtgc catcttctct acgtcgccct
     6361 attgtaggca taaatgcaat gagatcgagt ttattttgta attgttccca aagtgaaact
     6421 gcgctcaacc aatgccctgt ataatcttct accgactgta aaatctcaat gacattagac
     6481 cttgcggtaa gtgacagctc ccaagattta ggcataatta acctcaaaca aattttcatt
     6541 ttctcgtaat tgctgacgtt tctgcatatc cgcaaataaa tgatttaatt cgttattgat
     6601 ttgttctttg gtaaaggttt ctcctcgagc tattgcttct tgaccagctt taatttgctc
     6661 tgcaagaaac tcatcatatc cttgttcttt gaatagcata ttctgctcct ttgatttgtg
     6721 tcttgattcc actatgttca agacatagtt taagtttaaa tctacgctca aagcaatgaa
     6781 gagtcaaggg aatccttatg ttgatagggt gtgcatattt ttcgagtcaa atgaattaaa
     6841 aatcatcatt acgatcaccc actcatagat tttatcttcc tatctaaggt catcttctta
     6901 tatcaaacaa gcggtctaaa tttgcaaaaa aatgacaaat ttagaccgct tgtgagttac
     6961 aaaaatataa actaattgag cttatcaatt actgcgcata actaaatact ttcaccactt
     7021 tggttactcc acctacattg cgagcggctt cggcggcggc atcagcttgg ttttggctga
     7081 ctttgcccat taaaaacact tcgccgtttt cggtgacgac tttcacttcg gtggctttta
     7141 cctcgctatt tactaacagt ttggatttca cttgggtggt aatccagctg tcttgtgtaa
     7201 tctgtccaaa gccgattttt tcgccttggc ggatttcgtt gtaaatttcc gtcacgccct
     7261 ccgccccagc agcgaggttt ttcgcatttt ccactacgtc catacttggt gcttgcccga
     7321 ttaataacac tttgccgttg taagccacca cgttaatgcg ggcttcttgt ttgatttgct
     7381 cgtctttatt aatgttgtaa gcgacttttt cttctaaaat ttcatcatca acttgcgtgc
     7441 cggtggtgcg tgggtctgtc gccactttag ttgcaaccgc cgcagaagtc accaccgccg
     7501 tggtgataca accttgtaaa gagataaaac ctaatactaa taggctgccc tttaatagtt
     7561 tatttgctaa tttgttcata atctctcctt acgaaaatag taaatggtcg ataagttcac
     7621 ataataaatt cacataaaaa tgatggcttt caatcaccct cagttgattg gttgaagggg
     7681 ctgaaatttc aatatcactt tcatctaata agccgagtgt gtggtcgtta ttactgcctg
     7741 ttaaagcaat aatatctaaa tctaaatgtt gatttttcgc cgtatgaata gcttgcaata
     7801 tacattcttc attacccaca ggcgaaaaaa caatgaagag atcaccctct tttgcaaccg
     7861 cctgcaactg ttttttataa acagcctcta actcgttatc ttgtgccaca actcctgcca
     7921 atacaccgct taattgcagt tgtacggcag caaaactcgg tcttgccagt tcataacggt
     7981 gaagtaagtg gctgaccaat aattcggcat taccgtaaga gcgaccgtgt ccgcagacga
     8041 taactttatc gccacgcaac aaacaggcaa caatgcggtt tgccgcttca cttaatttag
     8101 ccggcaatag ctcagaggcg gaaatttggg tttgaatact ttcggtaaat ctatcttgga
     8161 ttttatttaa cattctttct aacctaaaaa gttgggaatc cacacaggcg tttggtttcc
     8221 ttcaaaagca accacatcaa aacggcaatt tgcagtttct aaactttggt tttgtttaag
     8281 caaccacata ttagcggcat tttgccattt ttgttgcttg cggtaatcaa tactctcaac
     8341 ggctgaaccg aagcggttgc ttttacgctg gcgaacttct acaaacacaa cggtttgtcc
     8401 gtcttgcata atcggatcca gttcgccacc tttaaatgct tggttagcgg caacaaaacg
     8461 aagcccttgc tgttctaaaa acactcgggc tttttgttca taaaacgagc cttgttcccg
     8521 tttattaata agcttgttga atagctccat tgcgatattg catccaagat aaactacgtt
     8581 caatattaca atgttgtccg gcttttaact caccggttaa acctgaaatt ttgtagcccg
     8641 gaatatggcg gaattcattg aatttggttg ccaatgacca cgcatcagcc cccattgcgt
     8701 ataaacgcat cattgagaaa tcgctattgg ctaaattctc ggctttttta tattcactag
     8761 aactttgatc agctaacagt ggcacttcac taaattttac cccttccatt gaagtgtaaa
     8821 aatcaatgcc gttatttggc gaattactgc gagatgaggt atagatcgct aaacgatctt
     8881 ttaaggttga gctatcaata ccttgtttaa tttctaatac ttgctcggca ttccccaaaa
     8941 tatataaagc tgctttgctc acacctgcat tttgaatagc ggcaacggca tcaagcggtt
     9001 ggttgtagta acgcacatca gcatcgctat tggtaagctg acgccagcgt ttggcaaagg
     9061 cttctgccga gcggttgccg tagtcatctt gaggggccgc aacgatagcg acactattac
     9121 cgtcttgata aattttttcc gcccctgatt gtgcctcaga ttcaggagat aaaccgtagt
     9181 aacacacctg agcaatatta cgcacatttt cagtggcatt cagtgccaaa atattcacgt
     9241 tttggatttc tgggctaagt agcatttcat caacacgaga tttgagcaat ggcccgacaa
     9301 tcacatttgc cccttgctgt tttgcctgtg aaattaagga attaacatca gatgaatcgg
     9361 tatcaaaagt ttgcactgcg gttgagtcat cagccttagc atcattaaaa ccacgcttga
     9421 taatatcacc caaaattttt gcatcaccac ttagaggcaa tagcaatgcg acactgttta
     9481 actgggtttg ttggaaatta ctcacccctt gtaattccgt tggcattacg gcaaccgcac
     9541 tatggctcgg gtattgggct ttccaattat taattgcttg tggcatttgt gccggtgtcg
     9601 tcacattttg attatagata ttaattaatg ccaaccaacc agcaagctcc atctcacccg
     9661 cttcaggcac agttttttcc agcattcctc gatttgcatc acgcaaggtt tgccaaataa
     9721 tatcgttatt ttcttggcgt tcacggttca ttttatagaa attgttaagt tgcgatcttg
     9781 cacgcacaat gccaatcaca tctttctgat tggcagcaat acgggcttgg gtttgaatat
     9841 aacgcaaacg ctgtgccgaa cttaatagcg gatcttgaat ggcttttaat tggcttgttg
     9901 cttgctcgtt tttcccttgt aaggcggcaa gctgggcgga aaccaaggca tactctaact
     9961 tttgcacctc gttcatctct gcggtcagaa tttcactaaa ggtattttga gcctcatatt
    10021 ccttattttc atcaattaat ttgcgtacag ctaaaagttg atacgaaatt ttatcttccg
    10081 cttctttggt ttgatctgct ttattaatat agaattccga gcttgaatag gcttcatttt
    10141 taatggattc ggtgacagga tttaccgatg tacaggcaga aatgaataat gcaatcgccg
    10201 ttggcaccaa tgcctttttc attgtatggt ttaaaatagt tgccattgtc agatcctcaa
    10261 tagaatataa tgtgctaaat ttaatgtgaa atcttactta tcaaacgagc taaaagcaaa
    10321 tgaaaaacag acaaaattca tctggaatca acccaaatta cggaatttta tatattgttg
    10381 ccacgcctat cggcaattta ggcgatatta cccaacgggc gttagacacc ctttcatctg
    10441 tcgatttagt cgccgccgaa gacactcgcc acagcggttt attgctgagc cactacggca
    10501 ttaaaaaacc gtttttcgcc ttgcacgacc ataatgaaca gcagaaagcg gtcgttttag
    10561 tcgaaaaatt agcaaaaggg gaaaacattg cgttaatttc cgatgccgga acgccactga
    10621 ttagcgaccc gggctttcat cttgtccgtc attgccgcca agccggggta aaagttgtgc
    10681 cgatccccgg ggcttgtgcc gctgttactg ccttatgtgc ttcaggcata gcttcagacc
    10741 gtttttgttt tgaagggttc ttaccggcaa aaacaaaatc ccgttgcgat aaacttaccg
    10801 agcttgaaaa cgagcctcgc acactgattt tttatgaatc cacccaccgt attttagaca
    10861 ctcttgccga tatgcagact gtttttggcg aagatcgcta tgtggtaatg gcgcgtgaaa
    10921 tcacgaaaac gtgggaaacc atttatggag atcaactggg taacttaatt gaatggctaa
    10981 atgaggatag caaccgaatt aaaggcgaaa ttgttctggt ggttgaaggt aaaccgcaaa
    11041 gtagtgagga agccttttcc agccaagcga tcaaattact gacattactc tgccaagaac
    11101 ttccgcttaa aaaagccgct gccattgtgg cagaaacctt cggctataag aaaaatgccc
    11161 tgtatcaata tggattggag cattttgagt aacagacaag cggtcagatt ttgtgagttt
    11221 tttgcagatt ggcactcccc tctctctgaa aaatgtttct gaacgaaaca tttcccggtc
    11281 tcaatactcg ctacgctcgc tccttcggag ccgttggcaa agccaacgtt caaatgtaaa
    11341 catttgtccc ataaggggag agagctaaat tattcgcttg tcgacccctc tccactcgtg
    11401 ggagagggac agattcttct tcgttcagaa gaaaaatcag ggagaggggg aagtttctcg
    11461 cactaaattc cgataagcat atgccataat cgcaaacatc aacagcaagt aaagcggtaa
    11521 taattgaatg ctgatagcat tagcaataaa gccgaaaatc ggtggcaata acaggacgcc
    11581 gatataggca cttgccattt gcacgccgat caccgattga gacttgtcct cgccgaaaaa
    11641 cgcaggggtc gaatgaatta agcacgggaa aatcggggca caacctagcc cgataataat
    11701 taaacttgcc agcgtaacat atttgcctaa cggcaagcac agtatcgcaa tgccgactaa
    11761 gatcaaggct gaaccaagtt taatcatttg tctgttgcta aatttaagcg tcaagaagcc
    11821 acttacgcct ctgccaacgg taatgccgat aaagaaaata ctgccgaacg cagccgcttg
    11881 ttctaagccg acatctttaa ccattacaaa atagctactc gcccacagtc cggcggtttg
    11941 ttctacgcca caatagccaa aaaaagctaa aatgccatat ttcacgcccg gaatttttag
    12001 cgtttctttt aagctcatcg gttttgccga atggttgatt tcagcggtaa tctgcttgcg
    12061 taatttccaa aaaggcagtg agcaaaacag cattagcgtt agcccgattt gtaacattga
    12121 aatggtgaaa tagcctgcct cccagcctcg cccttgcaat aaggcgtagc ctaaaatgtg
    12181 cggcccgata gaagctccaa cgccccacat acaatgcagc cagctcatat ggcggcttgc
    12241 gtagtgaact gctacatagt tattcaacgc cgcatcaacc gctccagccc ctaagccgta
    12301 aggaattgcc cataaacata agatccagaa agcatcactg attgaaaagc cccacaatgc
    12361 taaagccgtt aaggcaacgc tgatgaccat tactttgcct gtgccgagat ggtaggtcag
    12421 tttgtccgac attaagctag aggtaatagt gccaatcgac ataatcatcg agaccacacc
    12481 ggcataggaa agcggaacag caagctgttg gtgcattgac ggccacgccg agcctaaaat
    12541 tgcatcaggc aacccaaggc tgataaaaga gagataaata atagcgagta ggaggttagt
    12601 catagcttac aatcggtcac aaaaagcatc gctttttgaa cgttggctct gccaacggga
    12661 gcgaagctgt gaacaatcct tacaaggatt gtgaataatt tttgataaat tttttgtaaa
    12721 aaaatggctt aaggagacct taagccaaat tttattattt cacttctgcc aaataggcct
    12781 tattagcacg gtcaaaggtg tctaatacgg ctttgcttgg cattggcgtg ataagtgata
    12841 ccacaatgat cattagggta gcagcaataa agcccgggat catcgagtaa atttccggta
    12901 aggcgagctt aagtgtaata tcgccccaaa aatgcacgat taacgcaccg gtcagcattc
    12961 cggcaatcgc cccaaaaccg ttcatacgtt tccagaatag tgaaagcaat accacaggcc
    13021 caaatgccga gccgaaacct gcccacgcat tacgcactaa gccaaatact ttgctgtccg
    13081 ggtcttgggc aatataaata gctaatgctg ccaccgcaag caccatcaag cggcttaacc
    13141 aaaccagttc tttatcggag gcttgagggc gtaaaatgcc tttgtataaa tcttcggtaa
    13201 tcgcacttga acaaatcagt aattgagcgg aaagggtact cattaccgca gcaagaatgg
    13261 cagataataa aatgccggct acccaagggt taaataacaa tttagctaat tcaatgaaaa
    13321 tttgttccgg atttttgctg atattgccga cttgctccgg gttgccaaag aaatatgcca
    13381 taccaaaata accaatgcct attgccccca gtaagcagat aatcatccaa gtcatactga
    13441 tacggcgtgc attttcaaga gacttcacac tttctgccgc cataaaacgc accacaatat
    13501 gcggttgtcc gaaataaccc agcccccacg ccattaagct gataatgcct aatacgctgg
    13561 tgccggtgaa aatgtcgttg tagtcttttt gtgccatctc gcctgcacgg tcgattaagg
    13621 cttgcatttc atcaaaacca cctaagttat acatcacaaa aaacggggtg agaatcaggg
    13681 caaaaaccat taatgaagct tgaatagtat ccgtccaact tactgctaag aaaccaccaa
    13741 taaaggtgta aagaatggta gcaacagccc cataccaaat tgcttggtgg taaggtaagc
    13801 caaataagtt ttcaaatacc ctcgcaccgg ccacaacgcc tgatgcacag taaattgcaa
    13861 aaaagagtaa aaaaatgacc gctgataaaa tcttaattaa acgggtttta tcgccaaaac
    13921 ggttatgaaa atattcgggt aaggttaagg catcgccact gtgttctgta tgcgagcgta
    13981 atctgcctgc gactaatttc cagtttagat aagcaccgat agttaaaccg attgcgatcc
    14041 agccttcgat caaaccgcct gcataaacgg ctccgggtaa tcccatcagc aaccagccgg
    14101 acatatctga tgcaccggca gaaagtgcgg tcacaaagct acctaaacgg cgaccgccta
    14161 aaatatagtc tgaaaggtta ttggtaacac gataggcaat aaagccgatg gcaatcattc
    14221 ctagaatata gacaacaaac ataattgttg ttggatcaag accaaacatt attaatgaat
    14281 cctcaagatt tcttattgta taaagattaa agtgagtaaa gggtaaaaga ttacctgaaa
    14341 taatcaaaat ggcaacacaa aattaccgtc cgcccagctc agctgaaatt ttttctgctg
    14401 tttcttgaat agccggtagt agattttccc gccccagctg atttaaacga gcagttgaaa
    14461 gcgaaacaga aatcgcataa tggacttttt gttggtgatc aaaaatcggg caagcaatac
    14521 aagaaacccc taattcattt tcttcgttat ccattgcaaa accttgacgg cgaatggttt
    14581 ccagctcggt ttgcattccg tcaatcgtgg tgatggtatt gaccgtcagt ttttgaatga
    14641 tgtccgattt agtctgccaa tattgtggaa tgtaatctgc cgaaccataa gcaagaaaca
    14701 gttttcccat tcccgagcaa tagagttgca tttgttgccc gatataggct cgggtacgca
    14761 tcattccggt ggtagcttct aatttattga ttaaaatagc gtgatcgttt tcccgcatcg
    14821 caaaatttac cgtctcgcct aaggttaaat ttagggcttc taaatgtggg gcggcaatat
    14881 gcaaaatgtt aatcgaactt aaggctcgct gcccgattgc caaacatttg gtggttaaac
    14941 gataactgcc cgcagtcggt gcagggcgga cgtagccctc ttgttgtaag ccttgtagca
    15001 gacggtgaac cgtgcttttg ttcagccccg aaagctcggc taaatgggca agcggacagc
    15061 cgtttgggaa tttcgctaac aattccaata gtgttaagcc acgaattaaa ctttgatttc
    15121 cactcgattc cttttctttc tttgcattca tttggctgct cccaaaataa tatgatctaa
    15181 cggcatatcc caactctcaa tcggtaattg ttcgacttgc tgacattcgt gtgccaaacc
    15241 gacactaatc agatgaggtg cttgagcaag ggttctatca taaaagccac ctcccattcc
    15301 cagccgattt ccggctttat cacaagcaac aagtggggta aaaatcatct ctaattcttg
    15361 caacggaagg atatttctaa catctaatat cggctcttga atccccaagc ggttaaattt
    15421 tagaatagtt ttgcaattgt aatttacaaa caataattga ccgcttgtaa aagggtgcaa
    15481 cagcggcaga tacagctttt tgccctgttg aagtaattgt tccattaaag gaataggaga
    15541 aatttcaccg ttaaaaggca aataaaaggc aatatgtgag acttggtaag attcaatcaa
    15601 tgaaagagct tgaggaataa tggattcggc tgcttgagtt tgctgaccgg cagttaaact
    15661 ttgccgttta agccgcattg attgccgtaa ttggctacga agatcggtta aagatgattg
    15721 attcatataa agacccgaga tgccgctacc agtaatggtc cttgaaccag tcggttcaag
    15781 ggagtcaaac ttaactgctt ttaggcttct tactcctaat taaattaggg taagcttgct
    15841 caccagcgtt agatgtccaa ctcaaagttt caaattatcg gctcagggac gtaactcgta
    15901 acgcacaccc caggcaagac ttatagtaac ggaatttgat gatttttcct agtatttaat
    15961 cccacaaaat gcaaattctc aatctttttg gctctattcc taatcaattc gattaaaaat
    16021 tacctaattg gattgaaatt tgccaagcga attatctaac tgctcaatac aagccaccaa
    16081 cacattttta ttttctgcat tttggcgttg ggcttgctcc agttcataat ttaaattcag
    16141 tgccacaatc gctaacactt tttcaagctg aataatgccg gactgctctt tcaataccac
    16201 cacacgctct tccaaacgct gtgctgaagc cagtaaatgc tcttgctgtt caacagggca
    16261 ttgcaagcgt aaaacctgcc caaaaatctg tagttcgata ttatttgccg acataaatac
    16321 gaaatcctca cttaatcgca aaatttttgc tatattatgc cattctttag caaaatcaca
    16381 aataatccca ttatggctat cacagattcc caacaattta aacaactggt atcacaatta
    16441 caaattgatg atacaccggc agagtttcac ggttttttat gcggtttaat tgccggcggt
    16501 attcaagatg agtcttggaa aaccctcacc taccaattta ccaatgacgg acacgcattt
    16561 tccatcgagc cgttaaacac tttaaccgat ttttatcagc aattaagtga aagcttttca
    16621 gaagccaaca cgctgtttag cctttggttg ccacaaaacg aagaagatgg ttttgcctta
    16681 gctgatggta tctccgaatg gacaaacagt ttcttacttg gacttggcgt tgctcaacct
    16741 aaattacaac aagaaaccga tgaggttggc gaggcaatcg atgatttaga tgaaatcgcc
    16801 aaactaggct acagtgacga tgataataac gaggaactac tggaggctgg cgaagaagtg
    16861 ttggaatatc tgcgagtttt agccttattc ttgcacagcc attttgcttt aggcgtgccg
    16921 aaaaccgaag aaaagccacc attacactaa tgaataaata caagcggttc aatttcatct
    16981 ctattttgca aatatattat gcggggcgaa aaatatttcg cccctaccaa aatcaaagga
    17041 aaaaccaatg tcagaattaa aaaacgatcg ctatttaaaa gcgttattgc gtgaacctgt
    17101 ggatatgacc ccggtctgga tgatgcgtca agccgggcgt tatttgccgg aatataaagc
    17161 cacccgagca gaagccggcg attttatgtc actctgccgt aacgctgatt tagcgtgtga
    17221 agtcacctta caaccgctac gccgttatga actcgatgca gctatcctat tttccgatat
    17281 tttaactgtg cctgatgcaa tgggattagg tttaagtttt ggtgcaggtg aaggccctaa
    17341 atttgcccgc ccggtggcaa gcctcaatga tgtcaatcag ctaccgattc ctgatccgga
    17401 agacgagttg caatatgtaa tgaatgcggt gcgtaccatt cgccgagaat tgaaaggcga
    17461 agtgccacta attggttttt ctggcagccc ttggacactc gccacctata tggtggaagg
    17521 tggttcaagc aaagcattta ccaaaatcaa aaaaatgctt tacgctgagc ctcacattct
    17581 acacaaattg ctggacaagt tagctgacag tgtcatctta tacttaaatg cccaaatcaa
    17641 agccggtgct caagcggtga tggtattcga cacttggggc ggtgtattgg ctcacgagga
    17701 ttacaaagcc ttctcattac actatatgca caaaattgtg gacggcttaa ttcgagaaaa
    17761 tgaaggacgt aaagtgcctg ttaccctatt caccaaaggc ggaggcttat ggttagaagc
    17821 gattgcagaa acgggctgcg atgccgttgg gctagattgg acagtcgata ttgccgatgc
    17881 tcgccgccgt attggcgaca aagttgcgtt gcaaggcaat atggatccaa gcgtgctgta
    17941 tgcaagccct gaacgaatcg aacaagaagt aaaaaacatt cttgccggct tcggcaacgg
    18001 cagcggacac gtctttaatc ttggacacgg cattcattta gatgtgccgg tggaaagccc
    18061 gaaagtgttt gttgatgcga ttcacaacta ctcgaagcag tatcataaat aatggataaa
    18121 atgccctctc tccttgtggg agagggacag atttttcttt gttcagaaga aaaatcaggg
    18181 agaggggcaa gcggtcattt ttatacagaa ttttgcaaaa tcgttccctt ctctctgaaa
    18241 aataaagaag gaaatacaat gagaaacccg attcataaac gcatggaacg tttcgagcct
    18301 tggcaaaaca ccacatttat ggcgtgccta tgcgaaagaa tgtaccccaa ctatcagctt
    18361 ttttgtgaag taaccgaaca gcctgaaaaa gctaaggtat tccaaaatat tttaaattta
    18421 gtctgggaat atttaaccat aaaaggggca aaaattaatt tcgataacca actggaaaaa
    18481 ttagaagaaa tcattcctga tgtgaacgaa tatgcgtttt ttggtgtctt acctgcacag
    18541 gaagcttgcg aagccctttc tgaacttctt cacagtatta tcgctggttc aacccttgaa
    18601 caagcaatcc gcatcagcca aatttcatta ggcacagttg ccagctattt ggaaatgcaa
    18661 caagataaag aactgaacga gcaagagcta aagaattctg aagaaattca agaagaactg
    18721 gacgtacagt ggcaaatata ccgcttgcta aacgaatgtg aaaaacgtga tcttgatctc
    18781 atttttgaac ttaaagatga aatcagaagc agcgggatta gtaatatcgg gttaaatatt
    18841 aaccaatagc attgatattt gcaaaatctt gcttaataat actttgagaa atcttaactt
    18901 tgaattaagc tatcgcaagg tttcaataac ttctcagacg ttttacgtca tttattaaaa
    18961 acacatatct tagaggataa aactatgaac aaaactgagt taatcgatgc tatcgccgct
    19021 ggtgcaaact taaccaagaa agatgctaaa gcagcattag aagcaactct tgatgcaatc
    19081 tctgcaagct taaaagcagg cgacgctgtt caattaatcg gcttcggtac ttttaaagta
    19141 aatgagcgta aagcacgtac tggtcgtaac ccaaaaactg gtgaagagat caaaattgca
    19201 gcagctaaag ta
//
LOCUS       DANLXS010000026        19115 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.026, whole genome shotgun sequence.
ACCESSION   DANLXS010000026 DANLXS010000000
VERSION     DANLXS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19115
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.026"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            50..1000
                     /gene="tal"
                     /locus_tag="PXD71_000721"
     CDS             50..1000
                     /gene="tal"
                     /locus_tag="PXD71_000721"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDL6290874.1"
                     /translation="MSQLDTLSKMTVIVADTGDIEAIKLYKPKDATTNPSLILSASAL
                     PQYAPLIEEAINYAKAQSSDKDQQLVDAEDKLAVNIGLEILKLVPGRISTEVDARFSY
                     DTEKTIAKARKLIGLYNDAGISNDRILIKIASTWQGIKAAEQLEKEGINCNLTLLFSQ
                     AQARACAEAGVYLISPFVGRILDWYKAHEGKEYAPAEDPGVISVTNIYNYYKQHGYDT
                     IVMGASFRNVGEIIELAGCDRLTISPPLLKELQESDEKLVCKLDYKGEIKPRPEKMTE
                     AEFYWEHNQEPMAVEKLAEGIRKFAIDQEKLEAMLAEKLQ"
     gene            complement(1062..1335)
                     /locus_tag="PXD71_000722"
                     /pseudo
     CDS             complement(1062..1335)
                     /locus_tag="PXD71_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
     gene            complement(1366..1830)
                     /locus_tag="PXD71_000723"
     CDS             complement(1366..1830)
                     /locus_tag="PXD71_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL6290875.1"
                     /translation="MKKLALAVALMSSFALVGCANTDVFSGSVYSGEQAKEARSIAYG
                     TIVSVRDVKIQARSEGVIGTVGGGVLGGVAGNAIGGGTGQAIATAVGAVAGAVIGNQV
                     EQKASQVSSLEMVIRKDDGKEIVVVQKKEDGFVPGKRVRIVGSNSDLNVSLL"
     gene            complement(1851..2450)
                     /locus_tag="PXD71_000724"
     CDS             complement(1851..2450)
                     /locus_tag="PXD71_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL6290876.1"
                     /translation="MDKIIAENAKFFEKMTACFGDLSQFEQKIRQIAEIAGIDLAAYE
                     IDHLAVRMNQTETAEQWKILLLDGATLLKESEVNGRPIGLFALIQAVEFCGQFVKIVE
                     LPFPKNKVYLEEGWEHIELVFPMLDNESSEQWVSRALSHFRLADNSEIKLKISQPQVE
                     GERLPNPSIAISLRNVTFCNPYCLKLHPYDIKSVIMSEN"
     gene            2484..4211
                     /gene="argS"
                     /locus_tag="PXD71_000725"
     CDS             2484..4211
                     /gene="argS"
                     /locus_tag="PXD71_000725"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL6290877.1"
                     /translation="MNIQQILSDKIKQAMVASGADSNVEPLVRQSGKPEFGDYQANGA
                     MGAAKKLGMNPREFAQKILENADLNGIADKLEIAGPGFINIFLNPEWLAENTNSSLQA
                     VNFGIKTANPQTIVIDYSSPNVAKEMHVGHLRSTIIGDAVVRTLAFLGNNVIRANHVG
                     DWGTQFGMLIAYLEKMENEHASEMELSDLEAFYRAAKEHYDSDEAFAEKARNYVVKLQ
                     SGDKYCLTMWKKLVDITMHHNQENYNRLNVTLTEKDVMGESLYNPMLPEIVADLKAKG
                     LAVEDDGALVVFLDEFKNKDGDPMGVIVQKKDGGFLYTTTDIAAAKYRYETLKADRAL
                     VFSDSRQAQHMQQAWLITRKAGYVPDSFSLEHPFFGMMLGKDGKPFKTRSGGTVKLKD
                     LLDEAVERADKLIAERSTELTAEEKAAVVEAVAIGSVKYSDLSKNRTTDYVFDWDNML
                     TFEGNTAPYMQYAYTRIRSIFARANIDENALSGSIQLNEPKERALAVKLLQFEEALNG
                     VAKEGMPHILCQYLYELAGSFSSFYEACPILNAEETTKNSRLKLAALTAKTLKQGLDL
                     LGIKTVEKM"
     gene            4401..5567
                     /gene="cgtA"
                     /locus_tag="PXD71_000726"
     CDS             4401..5567
                     /gene="cgtA"
                     /locus_tag="PXD71_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDL6290878.1"
                     /translation="MKFIDEALIRVEAGDGGNGCVSFRREKFIPKGGPDGGDGGDGGD
                     VYLIADENLNTLIDYRFEKRYAAGRGENGRSAGCTGHRGQDITLRVPVGTRAIDNDTQ
                     EIIGDLTRHGMKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLKLELLLLA
                     DVGMLGLPNAGKSTFIRSVSAAKPKVADYPFTTLVPSLGVARVGADRSFVVADIPGLI
                     EGAADGAGLGIRFLKHLERCRILIHLVDIMPIDESDPAHNISVIESELYQYSEKLAEK
                     PIWLVFNKIDTIGEEEAAERAKDIAEQIGWEGDYYLISAATGQNVQALTRDIMDFIEA
                     NPREVEEENKAPEEVKFKWEDYHNEAMQNAVEEDWDDDWSEEDDEGIEFIYERH"
     gene            5652..6269
                     /locus_tag="PXD71_000727"
     CDS             5652..6269
                     /locus_tag="PXD71_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL6290879.1"
                     /translation="MLSSTSCDLFNIPFFQFAQMKKYCPEEIPRIKAEYKTQWEIWKK
                     LHQEVSRQLGQPFAPPHIEKWCNGWQVRAHFFAYYKYEFNRNSAAILSVILNRRRLQV
                     SLDWHCYRADRSQINVHQYNQWWENLDREKFADFEIWHGSESEYDDFRTVSQMSEQAF
                     TLTNEEDFWCIGKNVEKDDLDNIDVVEFIYQTIRALLPLYEKCHQ"
     gene            complement(6316..8274)
                     /locus_tag="PXD71_000728"
     CDS             complement(6316..8274)
                     /locus_tag="PXD71_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDL6290880.1"
                     /translation="MIAELGNYTLALALAISILLAVLPLWGAEKQNSTLIALARPMTW
                     AMFLALTVSFGSLFYLFAVNDFTVQYVVNNSNSRLPLQYRLSAVWGAHEGSLLLWVWL
                     LSLWSVGVATFTRKMPEDAVARVLGLMGVIGIGFLMFVIFTSNPFDRTFPNFPIDGKE
                     LNPMLQDVGLIFHPPLLYMGYVGFSVAFAFAIASLMTGRLDTAWARWSRPWTMAAWVF
                     LTLGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLAGTALIHSLAVTEKRGTFKAWT
                     VLLAILAFSLCLVGTFLVRSGILVSVHAFASDPTRGLYILAYLVLVVGGSLLLYAFKG
                     SEIKSLDNYERYSRETMLLINNILLMAFLSVVFLGTLLPLIHKQIGLGTISIGAPFFD
                     QMFLILMVPFSFVLGIGPLVKWRRDQVSAIRKPVIIATILMALLGFGLPYLFRNTITV
                     SVVLGTMMSVFIVLLSLYELHQRATHRHSFLSGIFKLSRSHWGMVLAHLGVAMTVFGI
                     AFSQNFSIERDVRMNVGDKAEILDYQFEFKGIKITDGANYQGGTAELEITRNGKYEAT
                     LNAEKRFYTVSRMGMTEAAIDWGFTRDLYAALGEKLEDNSWAVRLYYKPFIRWIWIGG
                     LFMALGGLLCMLDKRYRLRIENKKEVIA"
     gene            complement(8271..8828)
                     /gene="ccmE"
                     /locus_tag="PXD71_000729"
     CDS             complement(8271..8828)
                     /gene="ccmE"
                     /locus_tag="PXD71_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDL6290881.1"
                     /translation="MNPRRKSRLKVVVSILLGLSVAAGLTLYALSQNIDLFYTPSEIV
                     NGKNDDPKQKPEVGQRIRVGGMVVEDTVKRDDKSLKVVFDLNDIGPSITVEYEGILPD
                     LFREGQGIVAQGVLIEPTRLKATEVLAKHDENYMPPELGDKLKEQHKKSGVSEQDLKG
                     ESETDRKMKAEAEAEKANNVQGESK"
     gene            complement(9014..9211)
                     /gene="ccmD"
                     /locus_tag="PXD71_000730"
     CDS             complement(9014..9211)
                     /gene="ccmD"
                     /locus_tag="PXD71_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDL6290882.1"
                     /translation="MTFQFESISDFFAMGGYGFYVWLSYGVSFIAMIGLIWLSRREQK
                     AILAQAKKEIVREEKLKQTSQ"
     gene            complement(9226..9963)
                     /locus_tag="PXD71_000731"
     CDS             complement(9226..9963)
                     /locus_tag="PXD71_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HDL6290883.1"
                     /translation="MWKWLHPYAKSETQYHLLGKIQPFLGWLSLIMLAAAFVWGLAFA
                     PKDYQQGDSYRIIFIHVPAAIWSMGVYGSMAVAGLVALVWQIRQANLAMISMAPIGLV
                     FAFISLATGAIWGKPMWGTWWVWDARLTSALVLFFLYIGVMALYSAFQDRQTGAKAAA
                     VLSVVGVINLPIIHFSVEWWNTLHQGATITKFDKPSMAVEMLIPLLLAIFGSLIFTAW
                     FSIWRYRIALLKDERNRPWVKQLAKSV"
     gene            complement(10163..10825)
                     /gene="ccmB"
                     /locus_tag="PXD71_000732"
     CDS             complement(10163..10825)
                     /gene="ccmB"
                     /locus_tag="PXD71_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDL6290884.1"
                     /translation="MILLTIIQRELTIAFRKPSEILNPLWFFLMVITVFPILMGPNPE
                     LLGKIAGGIAWVAALLSALLSFERLFRDDYLDGSLEQLMLLPLALPQVALAKVIAHWL
                     LTGLPLILLSPIAAILLSLETHVWWALVLTLLLGTPILSCLGAIGVALTVGLRKGGTL
                     LSLLILPLFLPVLIFAAAVLEAATLNMGYSGQLAIIGAILAVTLTFSPFAIAGALRIS
                     LN"
     gene            complement(10822..11463)
                     /gene="ccmA"
                     /locus_tag="PXD71_000733"
     CDS             complement(10822..11463)
                     /gene="ccmA"
                     /locus_tag="PXD71_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDL6290885.1"
                     /translation="MNQTAQYQLKLNQIACERGETRLFEGCSFTVQSGEWVQIEGHNG
                     IGKTSLLRILAGLFLPAEGEVLWNDIAIQKQRDHYYSELFYLGHYSGVKPELTPWENL
                     RFYQKIQQLPQDDDALWHALTKVSLIGREDLPCSHLSAGQQRRVALAKLWLTNAKLWI
                     LDEPFTAIDKKGVADLIAHIEQHCEQGRLVIFTSHQAAESDKVRIISLDQFKV"
     gene            complement(11607..12317)
                     /gene="hda"
                     /locus_tag="PXD71_000734"
     CDS             complement(11607..12317)
                     /gene="hda"
                     /locus_tag="PXD71_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HDL6290886.1"
                     /translation="MQLPLPIHQIDEDTFDNFYSQNSEVLLGSLKRNFTDVQQPFFYI
                     WGGKSCGKSHLLKAVSNHFLLNNQTSSYIPLTKANYFSPLVLDNAELLNVICLDDIQA
                     IAGDAEWELAIFNLFNQIREQQGLFNEGQKTLLLISADCPPHQLNIQLPDLRSRLTWG
                     EVYQLNDLTDEQKCQILQLNAHQKGVELSDEVAHFLLKKAGTDLEQLSKILDKLDKAS
                     LQAQRKLTIPFVKEILSL"
     gene            12587..14188
                     /locus_tag="PXD71_000735"
     CDS             12587..14188
                     /locus_tag="PXD71_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6290887.1"
                     /translation="MKNMTKLSLITLALAASSSVFAAPKTFVYCLEASPSALSPVLST
                     DGASFDVNALPIYNKLTDFEDGTTNVIPSLAEKWDISEDGKTYTFHLRKGVKFHDNKE
                     FKPSRELNADDVVFSINRQFDPNHPFHKVSGGNYEYFIGMDMQNIIDKVEKVDDHTVK
                     ISLKVPNAPFLANLAMDFNAVYSAEYAEQSLKAGKPERIDTNPIGSGPFQFVDYQKDA
                     TVRYKAFENYWQGTAKIDRLVFAITPDASVRLAKLQKGECHAMPYPNPADIEDLKKDP
                     NIELMSQSGMNIGYLALNSSIKPLDNQKVRQALNHAVNKQAIVDAVYQGAGQVAKNPI
                     PPTMWSYNEAVQDYDYNPEKAKALLKEAGFENGFDTELWAMPVSRPYNPNARRMAELI
                     QEDWKKVGVNAKIVSYEWGEYLKRLRQGEAATSMIGWTGDNGDPDNFLNTLLSCSAVE
                     AGSNYAKFCHKPFEEVVLGAAQESDKAKRTELYKQAQVIFKEQAPWITIAHSTVYFPV
                     RKEVKGYVMSPFSLHNFYKVDLADK"
     gene            14460..15464
                     /locus_tag="PXD71_000736"
     CDS             14460..15464
                     /locus_tag="PXD71_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6290888.1"
                     /translation="MFSFILKRVLMVIPTFIAITLITFALVHFIPGDPIEIRMGERGL
                     SPEVHQQMMEQLGLNRPLFEQYISYIKGVLHGDLGNSFRNNEPVLKEFFTLFPATVEL
                     AFFALLWSLIFGIILGVIAAVKKESWISHTVTTLSLTGYSMPIFWWGLILILYFSTPL
                     GLPASGRLPSEYWIEAETGFMLIDTWNSDEPGAFLAAIKSLILPSIVLGTIPLAVVTR
                     MTRSSMLEVLGEDYIRTAKAKGLNTTRIVIVHALRNALIPVVTVVGLIVGQLLSGAVL
                     TENIFSWPGIGKWIIDAINSRDYPVLQGSVLIIATIIILVNLTVDLLYGIVNPRIRHS
                     "
     gene            15566..16459
                     /locus_tag="PXD71_000737"
     CDS             15566..16459
                     /locus_tag="PXD71_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6290889.1"
                     /translation="MSGITAVNPPAPKTPLQEFWYYFCQNRGALIGLTFIAIVFVACV
                     FAPWVAPFDPIEQNRSALLLPPAWLEGGNSAHLLGTDDIGRDILSRIIYGSRLSVFIG
                     LVIVLLSCVFGVILGLLAGYYGGTLDIIIMRFVDIMLAIPSLLLTIGVVTILGPSLIN
                     AAIAIAIVSIPSYVRLTRASVLSEKNRDYVTASRVAGASVLRLMFVVILPNCLAPLIV
                     QMTMGISNAILELAALGFLGIGAQPPTPELGTMLAESRGFMQSANWLVTIPGLAILSL
                     VLAFNLMGDGLRDALDPKLKQ"
     gene            16470..17459
                     /gene="dppD"
                     /locus_tag="PXD71_000738"
     CDS             16470..17459
                     /gene="dppD"
                     /locus_tag="PXD71_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDL6290890.1"
                     /translation="MALLTVDQLSVHFGETKTPFKAVDRISYEVNEGEVLGIVGESGS
                     GKSVSSLAIMGLIDFPGRVMAEKLYFNGNDLLALKPKEKQQIVGADVSMIFQDAMTSL
                     NPSYTVGYQIMEALKVHQGGSKASRRERAIELLTMVGIPDPESRLEVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIDLLLELQRKENMALILITHDLALVA
                     ESAHRIIVMYAGQVVEEGKAEQIFKAPLHPYTQALLKALPEFAEGKSRLQSLPGVVPG
                     KYDRPQGCLLNPRCPYATEKCRTIEPAMQIINGRQVKCHTPLNAQGEPIRIAV"
     gene            17587..18576
                     /locus_tag="PXD71_000739"
     CDS             17587..18576
                     /locus_tag="PXD71_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6290891.1"
                     /translation="MKENNSLPPFLKAVDLKKYYPVKKGMFAKPKTVKALDGVSFTLE
                     RGKTLAIVGESGCGKSTLGRMLTMIESPTEGELFYNGQNFLVNDKETAQLRRKKIQIV
                     FQNPYSSLNPRKKVGSILEEPLLINTNLSAKERKEKVLEMMAKVGLKPEFYDRYPHMF
                     SGGQRQRIAIARGLMLQPDIVVADEPVSALDVSVRAQVLNLMMDLQEEMGLSYVFISH
                     DLSVVEHIADEVMVMYLGRCVEQGDTKTIFSNPRHPYTQALLSATPRLNPEQRRERIK
                     LTGELPSPLNPPKGCAFNARCRFATDRCYVERPELKTYQDGTRIACFVVEENN"
     gene            complement(18810..>19115)
                     /locus_tag="PXD71_000740"
     CDS             complement(18810..>19115)
                     /locus_tag="PXD71_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248476.1"
                     /note="Bacteria have multiple sigma factors which are
                     active under specific conditions; the sigma factor binds
                     with the catalytic core of RNA polymerase to produce the
                     holoenzyme and directs bacterial core RNA polymerase to
                     specific promoter elements to initiate transcription;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HDL6290892.1"
                     /translation="DDDEENHFFDNYGHWKEEYYPSSWQEEAVYSKEFWILFEVCLSN
                     LPAKQARVFMMREYLEATSDEICRVVNITSDNLYTLIYRARLQLQQCLSRKLTERNR"
BASE COUNT         5763 a         4197 c         3972 g         5183 t
ORIGIN      
        1 tagtaacttt tactactatt tatttcacaa tcaaaaaagg aatcagacta tgagccaact
       61 tgacacctta agcaaaatga ccgttatcgt tgctgacact ggtgatattg aagcaatcaa
      121 actttataag ccaaaagatg caacaacaaa cccgtcttta attttaagtg catcggcatt
      181 accgcaatat gctccattaa ttgaagaagc gattaattac gcaaaagcac aaagtagcga
      241 taaagatcaa cagttagttg atgctgaaga taaattagcg gtaaatattg gtttagaaat
      301 cttaaaactt gtacctggac gtatttctac agaagttgat gctcgttttt cttatgatac
      361 tgaaaaaact atcgctaaag cacgtaaatt aattggatta tataacgatg caggcatcag
      421 taatgatcgc attttaatta aaattgcctc tacttggcaa ggtataaagg cagcagaaca
      481 gttagaaaaa gaaggcatta actgtaactt aacgttatta ttctctcaag ctcaagctcg
      541 tgcgtgtgca gaagcagggg tttacttaat ctctccattc gttggtcgta ttttagactg
      601 gtacaaagct cacgaaggta aagaatatgc tccagctgaa gatccaggtg taatttcagt
      661 aaccaatata tacaattact acaaacaaca cggttatgac actattgtaa tgggtgcaag
      721 tttccgtaat gtaggagaga tcattgagtt agctggttgc gaccgtttaa ccatctcacc
      781 accgttatta aaagagctac aagagagcga tgaaaaatta gtttgcaagc tagattacaa
      841 aggtgaaatc aagcctcgtc cagaaaaaat gacagaagca gaattctact gggagcataa
      901 ccaagagcca atggcagtag aaaaattagc tgaaggcatt cgtaaatttg cgattgacca
      961 agagaagtta gaagcaatgc ttgctgaaaa acttcaatag acaacgcata aaaaaaccgg
     1021 cattaacgat aattaatgcc ggttttaatt tctataaatg attatttaaa ctcataacgt
     1081 ctaatcgttt ttagatcgct atcttcattt ttaatttcat cggcaagatg aacacgagca
     1141 ccaaccgctg catgtgcatc atactcacga gttagagtca cagtttcacc attatctcgt
     1201 tgaactgtaa gttcaacaac atcgtttgat actgctttgc tagataaatg ttgcattacc
     1261 tttatcagaa taaacatctg cagtacaagc tgctaacata agtgaagcta atacagctaa
     1321 agaaagtttt ttcattaata atatatcctc taggaaaaac taaaattaaa gcagagaaac
     1381 attcaggtct gaattagacc ctacgattct cacacgtttg ccaggcacaa aaccgtcttc
     1441 tttcttttga acgaccacga tttctttacc atcatctttg cgaataacca tttccaatga
     1501 agaaacttga ctcgcttttt gttctacttg attaccaatt accgcaccgg caactgcccc
     1561 cactgcggtt gcaatcgctt gccctgtacc accgccaatc gcattaccgg caacgccacc
     1621 taatacacca ccgccgacag taccgatcac accttcactg cgtgcttgaa ttttcacatc
     1681 acgcactgaa acaatagtgc cataagcgat agaacgagct tcttttgcct gctcaccgga
     1741 ataaacgctt ccgctaaaca cgtctgtatt tgcacagcca actaaagcaa aacttgacat
     1801 taatgcgaca gctaaagcta attttttcat tttgtttgtt ctccgaaacg ttaattttct
     1861 gacataatta cactttttat atcataaggg tggagcttta gacaataagg attacaaaaa
     1921 gttacatttc tcaagctgat cgcaatgctt ggatttggca atctctcccc ttctacctga
     1981 ggttggctga tttttaattt aatctcagaa ttatcagcaa gtctaaagtg cgacaatgcc
     2041 cttgacaccc attgttccga actttcgttg tctaacatcg gaaaaaccag ctcaatatgt
     2101 tcccagcctt cttctaaata aaccttattt ttcggaaaag gcaactcgac aatctttaca
     2161 aattgtccgc aaaattcaac cgcttgtatt aatgcaaaca gccctatcgg tctgccgttt
     2221 acctcgctct ctttaagtaa ggttgcccca tcaagcaaaa ggatcttcca ctgttctgcg
     2281 gtttcggttt ggttcattct taccgcaaga tgatcgattt catacgctgc taaatcaatg
     2341 ccggcgattt cagcaatttg tcggattttc tgttcaaatt gcgataaatc gccaaaacaa
     2401 gcggtcattt tctcgaaaaa ttttgcattt tctgctataa ttttgtccat ttttttgtaa
     2461 atagaattta aataggttaa acagtgaata ttcaacaaat tttatcagat aaaattaaac
     2521 aggcaatggt agcaagcggt gcagacagca atgtcgagcc tttagtccgc caatcgggca
     2581 agccggaatt tggcgattac caagccaatg gtgcgatggg tgcagcgaaa aaattgggga
     2641 tgaatccgcg tgaatttgcc caaaaaatct tagagaatgc cgatttgaac ggtattgccg
     2701 ataaattaga aattgccggt ccgggcttta tcaacatttt cttaaaccca gaatggctgg
     2761 cggagaacac taacagttca ttgcaagcgg tcaattttgg cataaaaact gcaaatccgc
     2821 agacgattgt gatcgactac tcctcgccaa acgtagcaaa agaaatgcac gtcgggcatt
     2881 tgcgttccac cattattggc gatgcggtag ttcgtaccct tgcgttttta ggcaataatg
     2941 tgattcgtgc caaccacgtt ggcgactggg gtactcaatt cggtatgctg atcgcttacc
     3001 ttgaaaaaat ggaaaatgag cacgccagcg aaatggagct aagcgattta gaagccttct
     3061 atcgtgcggc aaaagagcat tatgattcag acgaagcatt cgccgaaaaa gcccgtaact
     3121 atgtggtgaa attacaaagc ggcgataaat attgcttaac aatgtggaaa aaattggtcg
     3181 atattacaat gcaccacaat caagagaact acaatcgctt aaatgttacg ctcaccgaaa
     3241 aagatgtaat gggcgaaagc ctctacaatc caatgttgcc tgaaattgtg gcggatttaa
     3301 aagccaaagg cttagcggtg gaagatgacg gtgctttagt ggttttcctc gatgaattta
     3361 aaaataaaga tggcgaccca atgggcgtaa tcgtgcagaa aaaagatggc ggtttcttat
     3421 ataccactac cgatattgcg gcagcaaaat atcgctacga gaccttaaaa gctgaccgag
     3481 ccttagtctt ttctgacagc cgccaagctc aacatatgca acaggcatgg ctcattaccc
     3541 gcaaagcggg ctatgtgccg gacagtttca gccttgaaca cccgttcttc ggaatgatgc
     3601 taggcaaaga cggcaaaccg ttcaaaaccc gtagcggtgg cacagtcaaa ctcaaagatt
     3661 tattagatga agcggtagag cgtgccgata aactgattgc cgaacgttca accgagctaa
     3721 ctgcggaaga aaaagccgcc gtggttgaag cggttgcgat tggttcggtg aaatattctg
     3781 acttatcgaa aaaccgcacc accgattatg tgttcgattg ggacaatatg ctgacctttg
     3841 aaggcaatac cgccccgtat atgcagtatg cctacacccg cattcgctcc atctttgccc
     3901 gtgcgaacat tgacgaaaat gcattgagcg gttcaatcca actcaatgag ccaaaagagc
     3961 gtgcattagc agtgaaacta ttgcaatttg aagaggcatt aaacggcgtg gcaaaagaag
     4021 gtatgccaca tattttatgc caatatttat acgagcttgc aggtagtttc tccagtttct
     4081 acgaggcttg cccgattctc aatgccgaag aaacaaccaa aaacagccgt ttgaaactcg
     4141 ctgccttaac ggcgaaaacc ttgaaacaag ggttggattt actcggtatt aaaactgtag
     4201 agaagatgta atacaaactc tctccccttg tgggagagag accgcttatg agcgttcagc
     4261 gaaataagca gagagagggg acaagcggta atttttatta aaatttttac acattttccc
     4321 ctctccctga tttttcttct gaacgaagaa aaatctgtcc ctctcccaca aggggagagg
     4381 gcatataaaa ggaaacaaat atgaaattta ttgacgaagc cctgattcgt gtagaagcag
     4441 gcgatggcgg taatggctgt gtgagcttcc gccgtgaaaa atttatcccg aaaggcggac
     4501 ctgacggtgg tgatggtggc gatggtggtg atgtgtattt aattgccgat gaaaacctaa
     4561 acacccttat cgactaccgc tttgaaaaac gttatgccgc tgggcgtggc gagaatggtc
     4621 gcagtgccgg ctgtaccgga catcgtgggc aagatattac cctacgagtg ccggttggaa
     4681 cccgtgcgat tgataacgac acccaagaaa ttattggcga tttaacccgc cacggaatga
     4741 aaatgctggt ggcaaaaggt ggttatcacg gtttaggtaa tacccgcttt aaatcctcag
     4801 taaaccgagc tccacgccaa aaaaccaacg gtacgcctgg cgaaaaacgt gatttgaaat
     4861 tagagctgtt actgcttgcc gatgtgggaa tgcttggctt accaaacgcc ggtaaatcca
     4921 cctttatccg cagtgtctct gctgccaaac caaaagtggc ggattacccg ttcacgacgc
     4981 tggtgccaag tttaggggta gcccgtgtgg gggccgaccg cagttttgtg gttgccgata
     5041 ttccggggtt aattgaaggt gcagcagatg gtgcagggct ggggattcgt ttcctaaaac
     5101 acttggaacg ttgccgtatt ttgattcact tagtcgatat tatgccgatt gatgaaagcg
     5161 atccggctca taatatttcg gtgattgaat ccgagcttta ccaatacagc gaaaaactcg
     5221 ccgaaaaacc gatctggttg gtgtttaata aaattgacac catcggcgaa gaagaagctg
     5281 ctgagcgagc aaaagacatt gccgagcaaa tcggctggga aggcgattac tacttgattt
     5341 ctgccgctac cggtcaaaac gtgcaagctc tcactcgtga tattatggac tttattgaag
     5401 ccaacccacg cgaagtggag gaagaaaata aagcacctga agaagtcaaa ttcaaatggg
     5461 aagactacca caacgaagca atgcaaaatg cggtagaaga agattgggac gacgattgga
     5521 gcgaagaaga tgacgaaggc atagagttta tttacgaacg ccattagtgc aataatacaa
     5581 gcggttgttt ttaaacggag atttgcaaat ttccggagaa aaacaaccgc ttgtaggttt
     5641 aaggagagtt tatgcttagc tcaacatcgt gcgatttatt caatattccg ttcttccaat
     5701 ttgctcagat gaagaaatat tgccctgagg agatccctcg aattaaggcg gaatataaaa
     5761 ctcaatggga aatttggaaa aagctacatc aggaagtttc tcgtcagctt gggcagccgt
     5821 ttgctccacc acatattgag aagtggtgca acggttggca agttcgagcc catttttttg
     5881 cctattacaa gtatgaattt aatcgaaatt ctgcggctat tctttccgtg attttaaatc
     5941 gtcgccgttt gcaagtgagc cttgattggc attgttatcg agccgatcgc tcgcaaatta
     6001 acgtgcatca atacaatcaa tggtgggaaa atttagatcg ggaaaagttt gctgattttg
     6061 agatttggca cggaagtgag agtgaatatg atgattttag aaccgttagt cagatgtcag
     6121 agcaagcgtt caccctaact aacgaggaag atttttggtg tatcggcaaa aatgtggaaa
     6181 aggacgattt ggataacatt gatgtagtag aatttatcta ccaaaccatt cgggcattat
     6241 tgccacttta tgagaaatgt catcaataaa cactaaaaag agaggcgaac atcatttgtt
     6301 cgcccctttc aaaatttaag ctatcacttc ttttttattt tcaatgcgta aacggtagcg
     6361 tttatccaac atacagagta aaccaccgag agccataaac agcccgccaa tccaaatcca
     6421 gcgaatgaac ggtttataat acaaacgcac tgcccaagag ttatcttcta atttttcgcc
     6481 aagtgcagcg tataaatctc gggtaaagcc ccagtcaatc gccgcttcgg tcattcccat
     6541 tcggctcacc gtatagaaac gtttttcagc attaagtgtg gcttcatatt taccgtttcg
     6601 agtaatttca agctcggcag tgccgccttg atagtttgcc ccatcggtga ttttaatacc
     6661 tttaaattca aattggtaat ctaaaatctc agctttatca cccacattca ttctgacatc
     6721 tcgttcaatg ctgaaatttt ggctaaatgc aatgccgaac actgtcatcg caacccctaa
     6781 gtgagccagc accattcccc aatgtgaacg ggacagtttg aaaatgccac ttaagaacga
     6841 gtggcgatgg gttgcacgtt ggtgtagctc atacaggcta agtaacacaa taaagacaga
     6901 catcatcgtg cctaacacca cactcacggt aatggtgtta cggaataagt atggcaatcc
     6961 gaatccaagc aatgccatta aaatagttgc aataattacc ggtttacgaa tagcagaaac
     7021 ttggtctcta cgccatttca ctaatggtcc aatccctaat acaaatgaga aaggaaccat
     7081 taaaattaag aacatttggt caaaaaatgg tgccccgatt gaaatcgtcc ctaagccgat
     7141 ttgtttgtga attaatggca gtaaggtccc taaaaacacc accgataaaa atgccattaa
     7201 taaaatatta ttaatgagta gcatcgtttc tcttgaatag cgttcatagt tatctaagct
     7261 tttaatttcc gagcctttga atgcatacag cagtaaagaa ccacctacta ctaacaccaa
     7321 gtaagccaag atgtataaac cacgagttgg atctgaagca aaggcgtgaa ctgacaccaa
     7381 aatgcctgaa cgcactaaga aagtccccac taaacagagc gagaaagcaa gaatcgctaa
     7441 taagaccgtc cacgctttaa atgtgcctcg tttttcagtc actgctaatg aatgaatcag
     7501 tgctgttcct gcgagccacg gcattaatga ggcattctcg acaggatccc agaaccacca
     7561 gccaccccaa ccaagttcgt agtatgccca ccaagaacca agcacgatac cgagcgttaa
     7621 aaatacccac gctgccattg tccacggacg agaccatctt gcccacgcag tatctaaacg
     7681 ccctgtcatc agtgaggcaa tcgcaaaagc aaaggcaacg gagaaaccca catagcccat
     7741 ataaagcaac ggtgggtgga agatcaagcc cacatcttgc agcattgggt ttaattcttt
     7801 gccatcaatc ggaaaatttg ggaaagttct atcaaacggg ttggaggtaa aaatcacaaa
     7861 catcaaaaag ccgatgccaa tgacccccat tagtcctaac actcgagcaa cggcatcttc
     7921 cggcatttta cgggtaaagg ttgctacccc gacagaccaa agcgacaaca accaaaccca
     7981 aagcaataat gagccttcgt gagcgcccca caccgcagat aaacgatatt gcaacggtaa
     8041 acggctattg gagttgttca ccacatattg cacggtaaaa tcattcaccg caaacagata
     8101 aaacagcgaa ccaaaagaga cagtcaatgc caaaaacatt gcccaagtca tcgggcgggc
     8161 aagagcaatt aaggtgctat tttgcttttc tgctccccaa agcggtaaaa ctgccaacaa
     8221 gatagaaatc gctagggcta atgccaacgt ataattgcct aattctgcaa tcatttgctt
     8281 tccccttgca cattgttggc tttttctgct tccgcctcgg ctttcatttt gcggtcggtt
     8341 tccgattcac ctttcagatc ttgttcagac acacctgatt ttttatgctg ttcttttaat
     8401 ttatcgccta attccggtgg catatagttt tcatcgtgtt ttgccagaac ttccgtagct
     8461 tttaaacggg ttggttcaat caacacgcct tgtgccacaa tgccttgccc ttcacggaat
     8521 aaatccggca aaattccttc gtattccacg gtaatcgaag gcccgatatc attgagatca
     8581 aatactactt ttaaactttt atcatcacgc tttaccgtat cttccactac cattccgccc
     8641 acacggatac gctgaccaac ttcaggcttt tgtttcggat catcattttt accgtttaca
     8701 atttcagacg gcgtgtaaaa cagatcgata ttttggctta acgcataaag tgtcaagcct
     8761 gctgcgacag aaagcccaag taatatcgaa accaccactt ttaatcttga tttgcgtctt
     8821 ggattcatcg taatcaccct tcaaaataaa tgcaaaattg cataaaaact caaaaataaa
     8881 ttagtgtaag aatagcaaaa aaccgcaaaa cattcccgat atttcaagga agttttgcga
     8941 tttctcaaat caaataccta taaagaagta tgaggctatc gttgcaaaaa ataaagttat
     9001 ttcgaccgct tgtttattgt gatgtttgct ttaatttttc ttcacgcaca atctcttttt
     9061 ttgcttgagc caaaatcgct ttctgttcac ggcgactaag ccaaatcagc ccaatcattg
     9121 caatgaaact gacaccataa gagagccata cataaaagcc gtagccaccc attgcaaaaa
     9181 aatcgctgat tgattcaaat tgaaatgtca ttgttctctc ctactctaaa ctgattttgc
     9241 cagttgtttt acccaagggc gattacgttc gtcctttaat aaggcaatgc gataacgcca
     9301 aatgctaaac caagcggtaa aaattaagct accgaaaatc gccaatagca acggaattaa
     9361 catttccacc gccatagacg gtttatcgaa tttagtgatg gtcgcccctt gatgcaaggt
     9421 gttccaccat tccaccgaaa agtgaataat cggcaagtta atcacgccaa caactgacag
     9481 cacagccgcc gctttggctc cggtttgtct gtcttggaat gcggaataaa gagccatcac
     9541 gccgatataa aggaaaaata acactaaagc ggaggttaaa cgagcgtccc acacccacca
     9601 agtcccccac attggtttgc cccaaattgc accggttgca agtgaaataa aagcgaatac
     9661 taagccgata ggtgccattg aaatcatcgc caagttagct tggcgaattt gccaaaccaa
     9721 tgccaccaag cctgccaccg ccattgagcc atatacgccc atcgaccaaa ttgctgccgg
     9781 cacgtgaata aagataatgc gatagctatc gccctgttgg taatctttcg gggcaaaagc
     9841 caagccccaa acaaaagcag ccgctaacat aatcaaactt agccaaccta aaaacggttg
     9901 gattttccct agcaaatggt actgcgtttc cgattttgca taagggtgta gccatttcca
     9961 catatatttc tccaaagtaa ataattcccc tcacccgttt acaggagagg gacgatttga
    10021 gcttcgttca gaagcgaaaa tcagggagag gggaaaaagt tgcaaatttt cataataatt
    10081 cgaccgcttc ccctctctct gaaaaacacc tctgaacgag gtgtttttcg gtctctctcc
    10141 ctcaagaggg agagaggaag ttttagttca aactaattct taatgcaccg gcaatcgcaa
    10201 acggcgaaaa agttaaagta accgctaaaa ttgctccgat aatcgccagt tgtccgctat
    10261 aacccatatt taaggtggcg gcttctaata ccgcagcggc aaaaatcagt accggtaaga
    10321 ataacgggag gattaataaa ctcagcaacg taccgccctt gcgtaagccc acagtgagtg
    10381 ccacgccaat cgctcctaag cagcttaaaa tcggggtgcc gagtaataat gtcagcacta
    10441 acgcccacca aacgtgggtt tcaagtgaaa gcaaaatggc agcaatcggt gaaagtaaaa
    10501 tcaacggtaa gccggtcagc agccagtgag caatcacttt cgctaacgca acctgcggca
    10561 aggcaagcgg cagtaacatt aattgctcga gcgaaccgtc taaataatca tcacggaata
    10621 accgctcaaa ggaaagcaat gcagaaagca aagcagctac ccacgcaatg ccgccggcaa
    10681 ttttgcctaa taattcgggg ttaggtccca ttaaaatcgg gaatacggta atcaccatta
    10741 aaaaaaacca caacggattt aaaatctccg agggtttacg aaaggcaatg gtgagttccc
    10801 gctgaataat agtcaataaa atcatacttt aaattgatct aacgaaataa tacgcacttt
    10861 gtcgctttca gcagcctggt ggctggtaaa aatcactaat ctgccttgct cgcaatgctg
    10921 ttcaatatga gcaatcagat ccgccacacc ctttttatca atagcggtaa agggttcatc
    10981 taaaatccaa agtttggcgt tagtgagcca caattttgcc aacgccacac ggcgttgctg
    11041 accggctgac aaatgcgaac aaggcaaatc ttcacgcccg atgagcgaca ctttcgtcaa
    11101 tgcgtgccaa agggcatcat catcttgcgg taactgttgg attttctggt agaaacgcaa
    11161 attttcccac ggtgttaatt ccggttttac ccccgaataa tgccctaaat aaaatagttc
    11221 agaataatag tgatcacgct gtttttgaat tgcaatatcg ttccacagca cctcaccttc
    11281 cgcaggtaaa aaaagcccgg ctaaaatacg taacaggctg gtttttccaa tgccgttatg
    11341 cccttcaatc tgcacccatt caccactttg cacggtaaaa ctgcaccctt caaacaaacg
    11401 ggtttcgccc cgttcgcagg caatctgatt aagtttaagc tgatattggg ccgtttggtt
    11461 cattattttt catctggttg aacattttag ccgaagtgta tcataagcct ttgatttaac
    11521 gtagcaaata aattacttct ttagaggtag caagcggtca aattttcaaa aagttttgca
    11581 aattttgata agaatcaaac cgcttgttac agagagagga tttccttcac aaaaggaatg
    11641 gttaatttac gctgggcttg gagagacgct ttatccaact tatccaatat tttagagagt
    11701 tgctctaaat ccgttcccgc tttttttaac agaaaatgag cgacctcatc ggacaattcc
    11761 accccttttt ggtgagcatt taactgtaaa atttggcatt tttgttcatc ggttaaatcg
    11821 tttagctggt acacttcgcc ccaagttaag cgggaacgta aatccggcaa ttggatattg
    11881 agttggtgtg gtgggcaatc ggcactgatt aacagcaagg ttttttgccc ttcgttaaac
    11941 aagccttgtt gttctcgaat ttggttaaac aggttaaaaa tagcaagttc ccattcagca
    12001 tctcctgcaa tcgcttgaat atcatctagg caaatcacat tcagcagttc ggcattatct
    12061 aacaccaacg gcgagaaata attggcttta gtgagcggaa tataactaga agtttggtta
    12121 tttaaaagaa agtggttact tactgctttc aacaaatgac ttttaccgca gctcttaccg
    12181 ccccaaatgt aaaaaaacgg ctgttgaaca tccgtaaaat ttcgtttgag ggatccaagc
    12241 aacacttcgc tgttttggct ataaaaattg tcgaaagtgt cttcatcaat ttgatgaatg
    12301 ggtagaggta actgcaaggc tttccttaaa tctctaagtt tgatattttt ctaagaatag
    12361 cggaaaagca ggggtttgag aagtaaaaat agtgagaaaa tatccactca tttttctaat
    12421 gaagagatga caagcggttg aatttccaca taattttgtc aatctgaccg aaaacccacc
    12481 gttttgtgtt aaaaaaagat tgcagttagc tcaaaaaatt gtatattcaa agctttctac
    12541 ttaaaaagta gagtaaaaca actattacaa acacaacgga gtgaatatga aaaacatgac
    12601 taaattatcg ctaataacat tagcgctagc cgcttcaagt agcgtctttg ctgcaccgaa
    12661 aacctttgtt tactgcttag aggcttcgcc ttcagcatta agcccagtgc tatcaacaga
    12721 cggtgcaagc tttgatgtga acgccctccc gatttataac aaattaaccg actttgaaga
    12781 cggtacaact aatgttattc cctctcttgc cgaaaaatgg gacatttcgg aagacggcaa
    12841 aacttacacc ttccacctgc gtaaaggcgt aaaattccac gacaataaag aatttaaacc
    12901 aagccgtgag ttaaatgcag acgatgtagt gttctccatt aaccgtcagt ttgatccaaa
    12961 ccatccattc cacaaagttt ccggcggtaa ctacgaatac tttatcggta tggatatgca
    13021 aaatattatc gataaagtgg aaaaagtgga cgatcacacc gttaaaatca gcttaaaagt
    13081 gccaaatgcg ccgttcttag ctaacttagc gatggatttc aacgccgttt actcagcgga
    13141 atatgcagag caatccttaa aggccggcaa gcctgagcgt attgatacca acccaatcgg
    13201 cagcggcccg ttccagtttg tggattacca aaaagatgcc accgttcgtt acaaagcctt
    13261 tgaaaactac tggcaaggca cagcgaaaat tgaccgctta gtgtttgcaa ttactccgga
    13321 tgcatcggta cgtttagcca agttacaaaa aggcgagtgc cacgcaatgc cttaccctaa
    13381 cccggctgat atcgaagatc tgaaaaaaga tccaaatatt gaattaatga gccaatctgg
    13441 aatgaatatc ggctatttgg cattaaattc atcgattaaa ccattagata accaaaaggt
    13501 acgtcaagca ttaaatcacg ctgtaaacaa acaagcgatt gtagatgcgg tatatcaggg
    13561 agccggtcaa gtagcgaaaa acccaattcc accaacaatg tggagctata atgaagcggt
    13621 tcaagactat gactacaacc ctgaaaaagc caaagcattg ttaaaagaag ccggttttga
    13681 aaatggtttt gatactgaac tttgggcaat gccggtatca cgtccatata acccgaatgc
    13741 tcgccgtatg gctgaactta tccaagaaga ctggaaaaaa gtgggcgtga atgcgaaaat
    13801 cgtcagctat gaatggggcg aatatctcaa acgcttacgc caaggtgaag cggcaacctc
    13861 aatgattggc tggacaggcg ataacggtga cccagataac ttcctcaaca ccttattaag
    13921 ctgctcagcg gtggaagccg gttcaaacta cgccaaattc tgccacaaac cgtttgaaga
    13981 agtcgtgctt ggtgctgccc aagaaagcga caaagcaaaa cgtaccgagc tttataaaca
    14041 agctcaagtg atcttcaaag agcaagctcc ttggattacg attgctcact ccaccgttta
    14101 cttcccggtg cgtaaagaag taaaaggtta tgtgatgagt ccgttcagct tacataactt
    14161 ctacaaagta gatttagcgg ataaataatc cgccaacaag cggtagaatt tgcagatttt
    14221 tttacaaatt tgaccgcttg tcctcccctc tttgaactac gaggggcagg ggggaaattt
    14281 ggcagaagcg attgccgaag tgagaggatt gtaaaagagg aaataaattt tctgtcttat
    14341 tgattatttt cttcgttaat tactactgtc aaatctcccc taccccctct ttgctaaaga
    14401 ggggaatcag atcgagagat cagacaactt tgatgaaaat ccaacctaag agattttaaa
    14461 tgttttcatt tattttaaaa cgtgtgctaa tggtgatccc tacctttata gcgattacgt
    14521 taattacttt tgcccttgtg cattttatcc ccggtgatcc aattgagatc cgaatggggg
    14581 aacgtggatt atcgcctgag gttcatcaac aaatgatgga acaactcggg ctgaaccgac
    14641 cgttgtttga acaatacatc agctacatca aaggtgtatt gcacggtgat cttggtaact
    14701 catttcgtaa caacgaaccg gtgctgaagg aattttttac cctattccca gccacggttg
    14761 agttggcatt ttttgccctg ctctggtcgt tgattttcgg cattattctc ggtgtgattg
    14821 ctgcggtgaa aaaagaaagt tggatttcac acactgtaac cacgctttca ctgaccggct
    14881 actcaatgcc aattttctgg tggggcttga ttttaattct ttatttctcc accccgctcg
    14941 gcttaccggc ttcaggtcgt ttaccatctg aatactggat tgaagccgaa accggtttta
    15001 tgctgattga tacttggaat tcggacgaac caggggcatt tttagcggca attaaatctc
    15061 tgattttgcc gtctatcgta ctggggacaa tccctcttgc agttgtgacc cgaatgactc
    15121 gctcttcaat gttggaagtg ttgggtgaag actatatccg caccgccaaa gccaaaggct
    15181 taaacactac ccgaattgtg attgttcacg cattaagaaa tgccttgatt ccggtggtaa
    15241 cggtcgttgg cttaattgtc gggcaactgc tttcaggagc cgttttaacg gaaaacatct
    15301 tctcgtggcc gggtatcggc aaatggatta ttgatgcaat caacagccgt gattaccctg
    15361 tactacaagg ctcagtgctg attattgcta ctattattat tttagtgaac cttacggtag
    15421 atctactcta tggcattgtc aatccgagga ttcgacatag ttagaaaaat tccttcgatt
    15481 tttcccctct ttagcaacga gggagttaca caagcggttc aaattccgca attttttaca
    15541 agattatttt tagttggagt taatgatgtc aggaataacg gcggtaaatc cacccgcacc
    15601 caaaacaccg ttacaggagt tttggtacta tttttgtcaa aatcgggggg cattaatagg
    15661 acttaccttt attgcaatcg tctttgttgc gtgcgtattc gcaccttggg ttgccccttt
    15721 tgatccaatc gaacaaaatc gcagtgcgtt attgctccct cctgcttggc ttgaaggggg
    15781 gaattccgct catctgctgg gtactgacga tattgggcgt gatattcttt cccgtattat
    15841 ttatggttca agattgtccg tctttatcgg cttagtcatt gtgctacttt cttgcgtatt
    15901 cggcgtgatt ttaggtctac ttgctggcta ctatggtggc acactcgaca tcatcattat
    15961 gcgttttgtt gatattatgc tggcgattcc aagcctgctt ttaactatcg gcgtggtcac
    16021 tattttaggc ccttcattga taaatgccgc tattgcgatt gcgattgtct ccattccaag
    16081 ttatgttcgc ttgacccgtg cttctgtatt aagcgagaaa aaccgtgatt atgttactgc
    16141 ttctcgtgtt gctggtgcga gcgtgcttcg cttaatgttt gtggtgattt tacctaactg
    16201 tttagctccg cttatcgttc agatgacgat ggggatctca aacgctattt tagaacttgc
    16261 cgccctcggc ttcttaggca ttggtgcaca gccgccaaca ccggagcttg gcacaatgct
    16321 tgccgaatct cgtggcttta tgcaatcggc aaactggctg gtgaccattc cgggtctagc
    16381 gattttatcg ttagtattag ctttcaactt aatgggtgac ggcttgcgtg acgccctcga
    16441 tccaaaactg aaacaatagg agatctaaaa tggcattatt aacggttgat caactttccg
    16501 tccattttgg tgaaactaaa acaccattta aagcggtgga tcgtatcagt tacgaagtca
    16561 atgaaggcga agtattaggc attgtgggcg aatccggttc aggaaaatcg gtaagttctc
    16621 tcgctattat gggcttaatc gatttccccg gcagggttat ggctgaaaaa ttgtatttta
    16681 acggcaatga tttattggca ttaaaaccaa aagaaaaaca acagattgta ggggctgatg
    16741 tctcaatgat cttccaagat gcaatgacca gcttaaatcc gagctatact gtgggctatc
    16801 agattatgga agccctaaaa gtgcatcaag gtggctcaaa agccagccgt cgtgagcgag
    16861 cgattgagtt gctgacgatg gtcggtattc ccgatcctga atcccgttta gaagtttatc
    16921 cgcaccagct ttcgggtggt atgagccaga gagtgatgat tgcgatggcg attgcctgtc
    16981 gcccgaaatt attgattgcc gatgagccga ctactgcgtt agatgtgacg attcaagctc
    17041 aaatcattga tttattactt gaattacaac gcaaagaaaa tatggcgttg attttaatca
    17101 cgcacgattt agcgttagtt gccgagtctg cccatcgcat tatcgtgatg tatgcagggc
    17161 aagtcgtgga agagggcaaa gccgagcaga tattcaaagc accactgcac ccttacaccc
    17221 aagcattgct caaagcctta ccggaatttg ccgaagggaa atcccgcctg caatcactac
    17281 cgggcgtagt gccgggcaaa tatgaccgcc cacaaggttg cttactcaac cctcgctgcc
    17341 cgtatgcaac cgaaaaatgc cgaaccattg aaccggcaat gcagataatc aacggcagac
    17401 aggtgaaatg ccacacgcca ctgaatgcac agggtgagcc gataaggata gctgtataac
    17461 gtcataaaga gtagggacgc aatgcttcgc gtccgttaca agcggtgatt tttaaccgat
    17521 ttttgcaaaa taacgatagt ggacgcaaag cattgcgtcc ctacattttc cgagaaggaa
    17581 aacagaatga aagaaaataa ttctctccca ccattcctca aagcagtgga cttaaaaaaa
    17641 tactacccag ttaaaaaggg aatgttcgct aaaccgaaaa cggtaaaagc gctggatggt
    17701 gtctctttta cccttgaacg tggcaaaacc cttgcgattg ttggcgaatc cggctgtgga
    17761 aaatcaactt tggggcgaat gctcactatg attgaaagcc caaccgaagg tgaactgttc
    17821 tacaacgggc agaatttctt agtcaatgac aaagaaacgg ctcaactacg ccgtaaaaaa
    17881 atccaaattg tgtttcagaa cccgtatagc tcgctcaatc cacgcaaaaa agtaggtagc
    17941 attttagaag aaccattatt gattaacacc aacttatccg caaaagaacg caaagaaaaa
    18001 gtgttggaaa tgatggcgaa agtcggctta aaaccggaat tttacgaccg ttatccgcat
    18061 atgttttcgg gcggacaacg ccagcgaatt gcgattgctc ggggcttaat gttgcagcct
    18121 gatattgtgg ttgccgatga acccgtttcc gcattagatg tttcggttcg ggcgcaagtg
    18181 ttgaatttga tgatggattt gcaagaagaa atgggcttgt cttacgtttt catctcgcac
    18241 gatttatccg tagtggaaca tattgccgat gaagtgatgg tgatgtattt agggcgttgc
    18301 gtagaacaag gggataccaa aactatcttc agcaatccac gtcaccctta tacacaagcg
    18361 ttactctccg caacccctcg tttgaacccg gagcaacgcc gtgagcggat taaactgact
    18421 ggcgaactac caagcccgct taatccacca aaaggctgtg cctttaacgc ccgttgcaga
    18481 tttgcaaccg atcgttgcta tgtggagcga ccagagctga aaacctatca agacggtact
    18541 cgcattgctt gttttgtggt ggaggaaaat aattaggcaa cgctcgctat ttaaaaggtt
    18601 aaagtttttc aactttaacc tttatttttc atacttaata ctcctcatct tttgtagcaa
    18661 aatctttcat taatttgctg atatgctcac aatggtgttt aaatgacaat acgggcaaat
    18721 aatcaaatgc gttttaacgc caatcttctc ttttaaagtg agttttcgtt cctttgagtc
    18781 agaaattaac cttgttactt gtcggcaatt catctatttc tctccgttaa tttccttgaa
    18841 agacattgtt gtaactgcag ccttgctcga tagattaatg tatataaatt atctgatgta
    18901 atatttacta ctcgacaaat ttcatctgag gttgcctcta aatattccct catcataaat
    18961 accctagctt gttttgcagg taaattgctt aagcaaactt caaataatat ccaaaattct
    19021 ttggaataaa cagcttcttc ctgccagcta gaaggataat attcttcttt ccaatgccca
    19081 taattatcaa aaaagtgatt ctcttcgtca tcatc
//
LOCUS       DANLXS010000027        18929 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.027, whole genome shotgun sequence.
ACCESSION   DANLXS010000027 DANLXS010000000
VERSION     DANLXS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18929
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.027"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            5..2440
                     /gene="plsB"
                     /locus_tag="PXD71_000741"
     CDS             5..2440
                     /gene="plsB"
                     /locus_tag="PXD71_000741"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDL6290893.1"
                     /translation="MSGLLNFYRAFLNLPLSLLVKSRSIPTHPVAELELNLEQPIVYV
                     LPYTSQTDLLILQKNCLALNLPDPLQENVIEGQTLLRFVFLDEGRRFFKSKGAKSETE
                     SIFYRYLDLHRANAELDVQLVPVSVLWGRAPGKEDARHLQVLTSFQRFLSMVWFGRDN
                     FVRFSPAVSLRYMVTEYGADEKIAQKIARVAKMHFAKLRYSAMGPRLPNRDAMFNKIL
                     NSEVIQAAIAEEAKKSSPEKARKEAEKIINEIAADVKHESLRVADRVLSWLWNKLYQG
                     INVQNADRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFWP
                     AGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEP
                     KTGMMSMSLQALQRGLNRSLSIVPVYIGYEHVLEVDTYAKELRGAAKEKENAGLVLRV
                     LKKLKNLGQGYVNFGEPILVNNYLNQYFPEWKEPSEDGRPKWLNEAVDNISHQVMVNI
                     NKAAAVNAKNLVGSALLASRQRALTREQLIEQLGSYIQLFRNVPYSKDMTLPTESAEV
                     MLNHVIHLPRSGVLIEKDNFGELVRLERESAVLMTYYRNNVQHLFVLPSLVASMVLHH
                     EAVSKDVVIKSVNRIYPFLQAELFLHFKQEELKAHIEAIIAELSRQGVIKNESDMLMI
                     NRSHIRTLQLHSAGVREILQRYYISLSVLLEQPNISRGYLEKESSSIAQRLSVLHGIN
                     APEFFDKAIFSTFTSSLKAQGYFDGDDNLAIAKVKETESLLRSLISIEVQQTIQGAMA
                     KVAESVENPNIAG"
     gene            complement(2545..3108)
                     /locus_tag="PXD71_000742"
     CDS             complement(2545..3108)
                     /locus_tag="PXD71_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDL6290894.1"
                     /translation="MSAPNSGTNPAPLGLCGFALTTFLLSLINNGTFSGENVGLVLAM
                     GFAFGGTSQMIAGMFEFAKGNTFGFTAFTSYGAFWWSFALFKVFFAANVSAEFVGWYL
                     VAWGTFTLMMFIGTLAKARALQAIFLSLTITFYLLAAGDFTHNTTLTNIGGNFGLLTA
                     FLAFYLAAADIINESFGKTVLPIGEPK"
     gene            complement(3306..4202)
                     /locus_tag="PXD71_000743"
     CDS             complement(3306..4202)
                     /locus_tag="PXD71_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6290895.1"
                     /translation="MDILDRLLAFAQISAGISVKCQLQGDWQLENPHQTGQAIAHIIS
                     YGQAYLQHNGKTHHLKQGDIVFFPRFADHTLRSQIQPLTSKNAVNPEQLGAFTLTQAG
                     LSQSECDMFCLHFYYDHQAELMESLPEMIVFQVAETPLKAMLDILKQEAETPNLAVKS
                     VVNSLAVVILTMILREHLVNHNNQLFGILKGWQDLHLRPLIKAILLNPEHEWQVDDMA
                     AFIHLSRAQLVRLFNKHLCISPHAFVHKIRLQKAAMLLKSQAGSVLSIALSCGFQSES
                     HFITAFKKQYCVTPSQYRQLNP"
     gene            4338..4679
                     /locus_tag="PXD71_000744"
     CDS             4338..4679
                     /locus_tag="PXD71_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL6290896.1"
                     /translation="MSSFQNWTEHTAHVKQSFGKLGQKHPKMLEAYKALGTAAAAEAL
                     DHKTRELIALAVAITTRCESCISVHAEEAAKAGATESEIAGALATAIALNAGAAYTYA
                     LRALEAVEEQK"
     gene            4690..5043
                     /locus_tag="PXD71_000745"
     CDS             4690..5043
                     /locus_tag="PXD71_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL6290897.1"
                     /translation="MKKLAWILGFAAMMPMMSSAKQASVQQVQAEKAAGIWIDVRSAE
                     EFQQGHLNGAINVTHTEIAQRIAEVAPDKNQPIHLYCRSGHRAEAALNELKKLGYSNV
                     TNHGGYDDLIKAGLK"
     gene            5103..5741
                     /locus_tag="PXD71_000746"
     CDS             5103..5741
                     /locus_tag="PXD71_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgB family protein"
                     /protein_id="HDL6290898.1"
                     /translation="MSAFNNFVNFVANIVAPMQRQFINFVRIAICIVMVWIGGLKVCQ
                     YEADGIAHFVSNSPFFSYMYKKGPNLVDDGTGKMVMEYTLHKNPEGKMVAKNIEWHKE
                     NGTYTASYIIGATIVTVGLLTLSGIWFPVSGMAGGLLTFGMSIVTLSFMITTPEIWVP
                     NLGGDMPTPAHGFPYLSAVGRLIVKDVIMMAGGLVAAAECANRILARRKAIA"
     gene            5876..7057
                     /gene="purT"
                     /locus_tag="PXD71_000747"
     CDS             5876..7057
                     /gene="purT"
                     /locus_tag="PXD71_000747"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDL6290899.1"
                     /translation="MTTIGTPLRTNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRY
                     DNAPAQQVAHRAYTISMLDGNALRDLVEKEKPDFIVPEVEAIATATLVELEQEGYNVI
                     PTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNFEQFQQAIQEIGIPCVVKPIMSS
                     SGHGQSVIKSEADIQQAWDYSQQGGRAGGGRVIVEGFIKFDYEITQLTVRHIHGTSFL
                     APIGHIQVDGDYRESWQPQQMSDIALKKAQETAEKITSALGGRGIFGVELFVCGDEII
                     FNEVSPRPHDTGIVTMASQELSQFALHARAILGLPIPEIYRISPAASKAIVVEGKSDN
                     VRFGGVDKVLAEIGTNIRLFGKGEVNGHRRLGVILARDENTVRALEKARRAYDKLDIQ
                     L"
     gene            complement(7149..8642)
                     /locus_tag="PXD71_000748"
     CDS             complement(7149..8642)
                     /locus_tag="PXD71_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL6290900.1"
                     /translation="MFKQLQQVGKAFMLPIAILPAAGLLLGIGGALSNKATVQAYPIL
                     DNPTLQGIFQIMASAGSVVFGNLALLLCIGLGIGLAKRDKGVAALAAVVGYLIMTGTI
                     SALIPLFPPETKGIDTGVIGSLVMGLITVKLHNKYHNIQLPQILGFFGGSRFVPIVTA
                     FAAIFVGAVFFLIWPAFQQWLVSAGQGIAEMGEIGTFFYGFLMRLSGAVGLHHMIYPL
                     FWYTELGGTEVVNGETIIGAQKIFFAQLADPTHQGLFTEGTRFFAGRFDTMMFGLPAA
                     CLAMYHAVPKARRGAISGLFLGAALTSFITGITEPIEFMFLFVAPWLYVFHAFLDGVS
                     FYIADLLNISIGNTFSGGVIDFTLFGILQGNENTNWLRVIIVGLPWAALYYFSFLFLI
                     KKFNVMTPGRAEESEEVVEQQTSSLTENAHKIIAALGSANNIENVDACITRLRVAVKE
                     VKAVDKATLKKLGAIDVLEVGGGVQAVFGAKAVLYKSEVNQILGKED"
     gene            8843..10690
                     /gene="ilvD"
                     /locus_tag="PXD71_000749"
     CDS             8843..10690
                     /gene="ilvD"
                     /locus_tag="PXD71_000749"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDL6290901.1"
                     /translation="MPILRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFT
                     QFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
                     RLDLVDAMIESADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSMLATHADRKELFLRAGREIVELCRKYYQQDDESVLPRSIATFDAFENAMSLDIAMG
                     GSSNTVLHLLATAQEAGVDFKMEDIDRLSRVVPCLSKIAPNTNKYHMEDVHRAGGIMG
                     LLGELDRAGLIHRHVKTVLGLTIEEQLNQYDIIRNKDEDLHKFFRAGPAGIRTTKAFS
                     QDCRWDTVDDDRVNGCIRNVENAVTKEGGLAVLFGNIAEDGCIVKTAGVDESIWKFTG
                     TAIVFESQEDAVAGILGGKVKEGHIVIIRYEGPKGGPGMQEMLYPTSYLKSMGLGKKC
                     ALLTDGRFSGGTSGLSIGHASPEAASGGAIALINDGDTVAIDIPNRSINLMVSDEELA
                     ARRAEMEARGDKAWQPLKREREVSFALKVFGHFATSADKGAVRDKSLLK"
     gene            complement(10824..11174)
                     /locus_tag="PXD71_000750"
     CDS             complement(10824..11174)
                     /locus_tag="PXD71_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HDL6290902.1"
                     /translation="MWHFYLMTIELNNIQYPTDASGYLTNLNDWSVELAEKIAEKEQI
                     SLTAEHWEIIYLVRDFYQEYKTSPAIRMLVKTMAQKFGEEKGNSRYLQRLFPDGPAKQ
                     ATKIAGLPKPAKCL"
     gene            complement(11203..11868)
                     /locus_tag="PXD71_000751"
     CDS             complement(11203..11868)
                     /locus_tag="PXD71_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor protein"
                     /protein_id="HDL6290903.1"
                     /translation="MENRLAHSYSTGESLISTHKVLRNTYMLLAMTLTFSAVVAFVAI
                     QMNAPAMPWWGLLVGFYGLLFLTNATSNSSAGILSVFALTGFLGYTLGPILNRYIGAG
                     LGDIVALAFGATALVFFACSAYVLTTKKDMSFISGMMVALFVVLLVGIIANIFLAIPA
                     LSLALSSLFVIFSSGAILLATSNIIHGGETNYIRATVDLYVSIYNLFVSFLQIFGVLG
                     SDD"
     gene            12074..12622
                     /gene="pgsA"
                     /locus_tag="PXD71_000752"
     CDS             12074..12622
                     /gene="pgsA"
                     /locus_tag="PXD71_000752"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL6290904.1"
                     /translation="MKLNFPTYLTLFRVGLTPLFIVAFYLPTKYAPEISTLIFFIASI
                     TDAFDGYLARKWNQTTRLGAFLDPVADKVLVAVAFVCVAEYYHTWWITIPICIMIARE
                     IIISALREWMAELGERASVAVSIWGKVKTTAQMLALGGMLWRQNQLMEILAFILLYIA
                     VGLTIWSMMQYLNASKNSLLKS"
     gene            12700..14538
                     /gene="uvrC"
                     /locus_tag="PXD71_000753"
     CDS             12700..14538
                     /gene="uvrC"
                     /locus_tag="PXD71_000753"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL6290905.1"
                     /translation="MSEFNAKSFLLNVPHQPGVYRMYDSKGTIIYVGKAKDLKKRLAS
                     YFRSQLSSKKTEALVANLANIETTITHSETEALLLEHNYIKENQPKYNVLLRDDKSYP
                     YILLTKHKHPRLTAFRGSKKIAGEYFGPYPNVTAVRETLNLLQKIFPIRQCEDTYYKN
                     RSRPCLQYQIGRCLAPCVEGCYSPVEYDQQVNLVRLFLQGKDHQVVEHLVQKMEDAAE
                     ALDFEGAARFRDQIQSVRAVQEKQFVSNDRLDDLDIISIVYQNGIACVHILFVRQGKV
                     LGNRSYFPKVPSNTDLTELADTFVGQFYLQMNQHRTIPNQIVIDHQLSESKALSEILS
                     EQAGHKVSILDNAIRGDKQRYLALARTNAMAALVLQLKQESHIQSRYQALKEMLKMSE
                     IKRMECFDISHTMGNQTVASCVVFDENGPLKSDYRRFNIEGITGGDDYAAMEQALLKR
                     YNRELPPEKIPDIIFIDGGKGQLNRALDVFAKLDVEWDKSKPLLIGVAKGVERKAGLE
                     TLIISKWDKEIHLPPDSLALHLIQHIRDESHQHAISGHRKKRQKAFTESGLESIAGVG
                     AKRRQALLKYLGGMQGVKSATLEEIQSVPGISKALAEVIFDTLKNS"
     gene            complement(14543..14818)
                     /locus_tag="PXD71_000754"
     CDS             complement(14543..14818)
                     /locus_tag="PXD71_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290906.1"
                     /translation="MKKLILLGLSVALTACSTPQQQSTVPMDMQAVQDYHQRVINKQT
                     VDKNTKQHDEPLNQSDKRSESINLPIHPIIYPSIGVGYHYGWKRYRY"
     gene            complement(14943..15296)
                     /gene="rplT"
                     /locus_tag="PXD71_000755"
     CDS             complement(14943..15296)
                     /gene="rplT"
                     /locus_tag="PXD71_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL6290907.1"
                     /translation="MARVKRGVIARARHKKVLKAAKGYYGARSRVYRVAFQAVVKAAQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGLSYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFAALVEKAKSAL"
     gene            complement(15405..15602)
                     /gene="rpmI"
                     /locus_tag="PXD71_000756"
     CDS             complement(15405..15602)
                     /gene="rpmI"
                     /locus_tag="PXD71_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL6290908.1"
                     /translation="MPKIKTVRGAAKRFKKTASGGFKRKQSHLRHILTKKTTKRKRQL
                     RHKSMVAKADQVLVVACLPYA"
     gene            complement(15866..16408)
                     /gene="infC"
                     /locus_tag="PXD71_000757"
     CDS             complement(15866..16408)
                     /gene="infC"
                     /locus_tag="PXD71_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL6290909.1"
                     /translation="MKPVKRVQSNRAHRLNDEIRGVKEVRLTDENGEQVGIVSLQDAL
                     NRAEEAELDLVEISPNAEPPVCRIMNYGKFIYEKEKAAKEQKKKQKVVQVKEIKFRPG
                     TDDGDYQVKLRSIIRFLEDGDKVKITVRFRGREMAHQEIGMDVLERVKADTAEIAMVE
                     SAPGKLEGRQVVMVIAPKKK"
     gene            complement(16567..18366)
                     /gene="recQ"
                     /locus_tag="PXD71_000758"
     CDS             complement(16567..18366)
                     /gene="recQ"
                     /locus_tag="PXD71_000758"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL6290910.1"
                     /translation="MNTTAISVLNNIFGYQKFRDGQEEVINAVLENRDCLVIMTTGGG
                     KSLCYQVPALCLDGITLVISPLISLMKDQVDQLITNGIEAAYLNSTQTLEEQQLVEQK
                     ALSGQLKLLYLSPEKVMTQGFFHFISLCKISFIAVDEAHCVSQWGHDFRPEYTLLGGL
                     RNTFPNVPLMALTATADLTTRSDILHHLRLNSPHIYLGSFDRPNIRYTVQEKFKPLEQ
                     LIKFISKQQGKSGIVYCNSRKKVEEITEKLSARKISVMGYHAGMSFEQRERVQNAFQR
                     DDIQIVVATIAFGMGINKSNVRFVVHFDLPRSIESYYQETGRAGRDDLPSEAVLFYDP
                     SDYAWLQKILLEEPESEQRNIKQHKLQAIGDFAESQTCRRLVLLNYFGEHRQEQCKNC
                     DICLDPPQQYDGLLDAQKVMSVIYRTGQIFGQHHIIAVLRGMNNLKIREHQHDQLSVY
                     GIGKEQSQHYWQNVVRQLIHLGLIIQNIANHSALQLTESARPFLRGEKQLQLALPRLG
                     ISATAFVQKQASHRYDKDLFARLRFLRKQIADLENIPPYIVFNDATLQEMAEFLPTSK
                     VEMLEINGVGERKFERFGQAFMSLIKEYQNNRK"
     gene            complement(18475..18780)
                     /gene="cyaY"
                     /locus_tag="PXD71_000759"
     CDS             complement(18475..18780)
                     /gene="cyaY"
                     /locus_tag="PXD71_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDL6290911.1"
                     /translation="MNTAQFHQAVEQVWQQIEEKIYDEGLSVDTEIQGAVCTLTFDDE
                     SQVIINKQEAMLELWLASKLGGFHFKNVEGEWVTDEGRSFWQHLEEAFARHGESVSF"
BASE COUNT         5488 a         3603 c         4142 g         5696 t
ORIGIN      
        1 ttatatgtct ggcttattaa atttttatcg agctttccta aacttaccac tttcactttt
       61 agtaaaatct cgctctattc cgactcatcc ggtggctgaa ctggaactaa atcttgaaca
      121 gccgattgtc tatgttctac cttatacctc gcaaaccgat ttattaattt tacaaaaaaa
      181 ctgcttggct ttgaatttgc ccgatccgtt gcaagaaaat gtgattgaag ggcaaacatt
      241 gcttcgtttt gtatttttag atgaaggtcg ccgttttttt aaatctaaag gggcgaaaag
      301 tgagacagag tcgatttttt atcgttattt agatttacat cgtgctaatg ctgaattaga
      361 cgtccaatta gtgccggttt cggtattatg ggggcgggct ccgggcaaag aagatgcccg
      421 ccatttacag gtgcttacct cattccaacg ttttttatcc atggtttggt ttgggcgtga
      481 taattttgta cgtttctccc ctgcagtatc gttacgctat atggtaactg aatatggtgc
      541 agatgaaaaa atcgctcaaa aaatcgcgcg tgtggcgaaa atgcactttg caaaattgcg
      601 ttattcagca atggggcctc gtttaccaaa tcgtgatgcg atgtttaata aaatcctgaa
      661 ttcagaggtg attcaagctg cgattgcgga agaagcgaag aaatcatcgc ctgaaaaggc
      721 tcgcaaagaa gccgagaaaa tcattaatga aattgccgct gatgtaaaac acgagagttt
      781 gcgtgtagca gatcgagttc tgagctggct ttggaataag ctttatcaag ggatcaacgt
      841 gcaaaatgca gaccgtgtgc gtaaattggc attagaaggt cacgagatcg tttatgtgcc
      901 ttgccaccgt agccatatgg actatttgct cctatcttat ctactttacc atcaaggatt
      961 agtaccgcca catattgccg ctgggatcaa tttaaatttc tggccggcag gcccgatttt
     1021 ccgtagttgg ggagcatttt tcattcgccg tacctttaaa ggaaatcgcc tctattccac
     1081 cattttccgt gaatatttag cggaattatt ctatcgtggt tattcggtcg aatattttat
     1141 tgaaggcggg cgttcacgca caggtcgttt attagagcct aaaaccggta tgatgtcgat
     1201 gagcctacaa gccttacagc gagggctgaa tagatcttta agtattgtgc ctgtttatat
     1261 cggttatgaa cacgtcttag aagtcgatac ttacgctaaa gagttgagag gtgcggcaaa
     1321 agagaaagaa aatgcgggct tggtgctacg agtgctgaaa aaactgaaaa atttaggtca
     1381 aggatatgtg aatttcggtg agccaatttt ggtgaataat tacttaaatc aatatttccc
     1441 tgagtggaaa gagccttccg aagatggacg acctaagtgg ttaaatgaag ccgtggataa
     1501 tatttcacat caagtaatgg tgaatatcaa taaagcggca gcagttaatg cgaaaaactt
     1561 agtggggtca gcattattgg catctcgcca gcgtgcatta acccgtgaac aactcattga
     1621 acaacttgga agttatattc agttatttcg taatgtacct tattctaaag atatgacatt
     1681 accaactgaa agtgcagaag tgatgttgaa tcatgttatt catttacctc gttcgggcgt
     1741 tttaattgaa aaagataatt ttggtgaact ggttcgttta gaacgagaat ctgctgtgtt
     1801 aatgacatat tacagaaata atgtgcagca tttatttgtg ttgccttcac ttgttgcaag
     1861 tatggtactt catcatgagg ctgtttcgaa agatgttgtg attaagtcgg ttaatcgtat
     1921 ttatccattc ttgcaagcag agttattctt acactttaag caagaagaat taaaagcaca
     1981 tattgaagca attattgcgg aactaagtcg gcagggggtg attaaaaacg aaagtgatat
     2041 gttaatgatc aatcgctcac atattcgtac cttgcaatta cattcagctg gtgtgcgtga
     2101 aatccttcaa cgttattata ttagcctaag tgtcttgttg gagcagccaa atattagtcg
     2161 tggctattta gaaaaagaga gtagttcaat tgctcagcgt ttatcagtct tgcatggtat
     2221 taatgcgccg gagttttttg ataaggcgat tttctctacc tttacatcaa gtctaaaagc
     2281 acaagggtat tttgatggtg atgataattt agcgattgca aaagttaaag aaacggaatc
     2341 gttgttacgt agtttgattt cgattgaagt tcaacagacc attcaaggtg cgatggctaa
     2401 agtcgcagaa tctgtagaga atcctaatat cgcaggataa taaatcaaag aagggcgcta
     2461 acaagcgctc gtttcttgca attttgttgc caaaaataaa agctgaatgc gcgaccacat
     2521 tcagcttttt tacttcctat ataattattt tggttcaccg attggtaaaa cagtcttacc
     2581 aaaactttca ttaataatat ccgcagcggc taagtagaac gctaagaatg cggttaataa
     2641 accgaagtta ccaccaatgt tagttagggt ggtgttatgg gtaaaatcac ctgcggctaa
     2701 aaggtagaaa gtaatggtta aacttaagaa aatagcttgt aatgcacggg cttttgctag
     2761 tgtgccgatg aacatcataa gtgtgaatgt tccccaagct actaagtacc aaccaacgaa
     2821 ttctgccgat acattcgcag caaagaacac tttaaataat gcaaaagacc accagaatgc
     2881 accatagctg gtaaacgcag taaagccgaa cgtgttacct ttagcaaatt caaacatacc
     2941 tgcaatcatt tgtgatgtgc caccgaaagc aaatcccatt gctaaaacta aacctacgtt
     3001 ttcaccgctg aatgtaccgt tattgattag gcttagtaaa aatgtagtaa gtgcaaaacc
     3061 gcataaaccg agcggagccg ggttagttcc tgaatttgga gctgacattg aaaacctctg
     3121 ttatattgtt acaccataag tgtggtaatt gtttatttac gccgataaat cagacgttat
     3181 gtgcctaaaa tttagggcga taaagtatag cgattttgca gtaaattgac tagaaatttc
     3241 cgagacaagc ggtgagattt ttgcaaaatt ttgccaaaaa atgaccgctt gttgttgtca
     3301 tttagctatg gatttaactg ccgatattgg ctgggtgtta cgcaatattg ctttttaaag
     3361 gcggtaataa aatgcgactc ggattggaag ccgcaagata aggcgatact taataccgac
     3421 ccagcttggc ttttgagcag cattgctgct ttttgtagcc taattttgtg tacaaaagca
     3481 tgtgggctaa tacataaatg tttgttaaat aagcggacta actgggcacg agaaaggtgg
     3541 ataaatgccg ccatatcatc gacttgccat tcgtgttcgg gattaagcaa aatagctttg
     3601 atcaaaggtc ttaagtgtaa gtcttgccaa ccttttaaaa taccgaataa ctgattgtta
     3661 tggttaacta gatgttctcg taagatcata gttaaaataa caacagcaag cgagttaacc
     3721 acagatttta cggctaaatt aggtgtttct gcttcttgtt ttagaatatc taacattgct
     3781 tttaatggtg tctcggcaac ttgaaatacg atcatttcag gtaagctttc cattaattcg
     3841 gcttgatggt cataataaaa atggaggcag aacatatcgc attctgattg agaaagtcct
     3901 gcttgggtga gtgtaaatgc acccaattgt tcagggttta ccgcattttt gctggtcaaa
     3961 ggctgtattt gactacgtaa tgtatgatcg gcaaatctgg ggaaaaatac aatatctccc
     4021 tgtttaaggt gatgggtttt cccattatgt tgtagataag cttgcccata gctgatgata
     4081 tgagcgattg cttgtccggt ttgatgcgga ttctcaagtt gccaatctcc ctgtaactga
     4141 catttcacac tgattccggc tgagatttga gcgaaagcta gaagcctatc taatatatcc
     4201 attgttcatt ccttatattt ccaccttaaa ttgcgattat ttcattataa tatgagctga
     4261 tttgataata aagtttatca gataaacgca ataataaatg cgttttttca tattattcac
     4321 tttaggagga aatcaatatg tcttcattcc aaaattggac agaacacaca gctcacgtta
     4381 agcaatcttt tggtaaatta gggcaaaaac accctaaaat gcttgaagct tataaagcat
     4441 taggtacagc ggccgcagcg gaagcattag atcacaaaac tcgtgaatta attgcattag
     4501 cagtagcgat tacaacacgt tgtgaaagtt gcatcagtgt acacgcagaa gaagcggcaa
     4561 aagcaggtgc aaccgaaagc gaaattgccg gtgcattagc gactgcgatt gcattaaatg
     4621 ccggtgcggc atatacctat gctttacgtg cattagaagc ggtggaagag caaaaataag
     4681 gagcaataaa tgaagaaatt ggcttggatt ttaggctttg cagctatgat gccaatgatg
     4741 tctagtgcta aacaagcatc agttcagcaa gtgcaggctg aaaaagcagc gggcatttgg
     4801 attgatgtac gttctgctga agaatttcag caagggcatt taaatggtgc aattaatgta
     4861 acgcatactg aaattgcaca acgtatcgca gaagttgctc cagataaaaa tcagccgatt
     4921 catctttatt gccgctcggg tcatcgagct gaagcggcgc ttaatgagct aaaaaaatta
     4981 ggttacagca atgtaactaa tcatggtggt tatgacgatc tgattaaagc cgggttgaaa
     5041 taactgtttt gagtgccaag catttgcttg gcacttttcg tttatatctg aggttaaaaa
     5101 ttatgagtgc ttttaacaat tttgttaatt ttgttgcaaa tattgttgct ccaatgcaac
     5161 gccaattcat taactttgta cgtattgcca tttgtatcgt gatggtttgg attggtggtt
     5221 taaaagtctg ccaatatgaa gcagacggta ttgcccattt tgtgtctaat agcccgttct
     5281 ttagttatat gtacaaaaaa ggcccgaatt tagttgatga cggcacaggt aaaatggtga
     5341 tggaatatac ccttcacaaa aaccctgaag gtaaaatggt ggcaaaaaac atcgaatggc
     5401 ataaagaaaa cggcacttat actgcatctt atatcatcgg tgcaaccatt gtgacggttg
     5461 gtttattgac gttatccggt atttggttcc cggtttccgg tatggcgggg ggcttactca
     5521 cctttggtat gtcgattgtt acactctctt ttatgatcac cacccctgaa atttgggtgc
     5581 cgaatttagg tggggatatg ccaaccccag cgcatggttt cccatatctg tcagcagtcg
     5641 gtcgtttaat tgtgaaagat gtgattatga tggcaggcgg cttggttgcc gcagcggaat
     5701 gtgcaaatcg cattttagcc agacgtaaag cgattgctta attgatagtg gtaatgcaag
     5761 cggtcatttt cttgtaaaat tttgcaataa aatgaccgct tgttttttgt caatttgcca
     5821 taatttcaag tataattcaa ttacgtaatc gtttgctttt atggagagga ataaaatgac
     5881 aacaatcggt acaccgttaa gaacgaacgc tacaaaagtg atgatgttag gttcgggcga
     5941 gttggggaaa gaagtggtaa tagagctgca acgcttaggc gtagaagtga ttgcggtgga
     6001 tcgttatgac aatgcacctg cacagcaagt ggctcaccgt gcttatacga tttcgatgtt
     6061 agacgggaat gcattgcgag atttggtgga gaaagaaaaa ccggatttta ttgtgcccga
     6121 agtagaagcc atcgcaactg caaccttggt tgagttagag caagagggat ataacgtaat
     6181 cccaacggca aaagcaacgc aattaacaat gaatcgagaa ggcattcgcc gattggcggc
     6241 agaagagttg ggcttaaaaa cgtcgcctta tcgttttgtg gataattttg aacagttcca
     6301 gcaagcgatt caagaaatcg gcattccgtg tgtggtcaaa ccgattatgt cttcttccgg
     6361 acacgggcaa tcagtaatta aatctgaagc ggatattcaa caggcttggg attattcgca
     6421 acagggcgga cgtgccggcg gtgggcgtgt gattgtagag ggttttatca aattcgatta
     6481 tgaaattacc cagcttaccg ttcgccatat tcacggcacg tcgtttttag ctcccatcgg
     6541 gcatattcag gttgatggcg attatcgtga atcttggcaa ccgcagcaaa tgtcggacat
     6601 tgcattgaaa aaagctcaag aaaccgcaga aaaaattacc tctgcactgg gcggaagagg
     6661 gattttcggc gtggaacttt ttgtctgtgg cgatgagatt atttttaacg aagtctcccc
     6721 acgtccacac gataccggaa tagtaacaat ggcatcacaa gagctttctc aatttgctct
     6781 gcacgcccgt gcgattttgg gcttaccgat tccggaaatt taccgcatca gcccggcagc
     6841 ttctaaagcg attgtggttg aagggaaatc ggataatgtg cgttttggcg gtgtagataa
     6901 ggtattagcc gaaatcggga cgaatatccg cttattcggt aaaggcgaag taaacggaca
     6961 ccgccgatta ggggtaattc ttgctcgtga tgaaaataca gtgagagcat tagaaaaagc
     7021 tcggcgagct tatgataagt tagatattca attataaagt caggattttg atattcgttg
     7081 atagtaggca ttttatgcct actatcctat aggcctctaa tcaaattgga tgaaaaagtt
     7141 gaagattgtt aatcctcttt gccgagaatt tggttcactt cgctcttata caatactgct
     7201 tttgctccaa atacggcttg tacaccgcca ccgacttcca gcacgtcaat cgcaccgagt
     7261 tttttcagtg ttgctttatc cacggctttc acctctttca ctgccacacg cagacgggta
     7321 atacaagcat ccacattttc gatattattt gcagagccaa gagcggcgat aattttatgg
     7381 gcattttcag tcagtgagct ggtttgttgt tcgactacct cttcactctc ttccgcacgt
     7441 cccggtgtca ttacgttgaa ttttttgatc aggaatagga acgagaagta gtaaagtgcc
     7501 gcccacggca agccgactat gatcactcga agccagttag tgttctcgtt gccttgcaaa
     7561 atgccaaaca gggtgaagtc gatcacgcca ccggagaagg tgttaccaat cgagatgttg
     7621 agtaagtctg caatgtagaa cgacacgccg tctaaaaaag cgtggaacac atagagccac
     7681 ggggcaacaa acaggaacat aaattcaatc ggctcggtaa tgccggtgat aaaagaggtg
     7741 agcgctgcac ctaagaacag accgctaatc gcaccacggc gggctttcgg cacagcgtga
     7801 tacatcgcta aacaggcggc aggtaagcca aacatcatcg tatcgaaacg tccagcgaag
     7861 aaacgtgtac cttcggtaaa taagccttga tgggttggat cggcgagttg ggcgaagaag
     7921 attttttgcg ccccgataat ggtttcacca tttaccactt ctgttccgcc caattcggtg
     7981 taccaaaaca gcgggtagat catatggtgt aagccgaccg caccgcttaa ccgcattaag
     8041 aaaccgtaga aaaaggtgcc gatttcgccc atttccgcaa tcccttgccc tgctgaaacc
     8101 aaccactgtt ggaaagccgg ccagatcaaa aagaacactg ccccgacaaa aatcgcagca
     8161 aaggcggtca caatcggcac aaaccgagag ccgccaaaga agccgagaat ttgcggcagc
     8221 tgaatgttgt ggtatttgtt atgcagtttc accgtaatca agcccattac cagcgagccg
     8281 atcacaccgg tatcaatccc tttggtttca ggaggaaaca gcggaatgag ggcagaaatc
     8341 gtgcctgtca taatcaaata accgaccact gctgccagtg ccgctacgcc tttatctcgt
     8401 tttgctaatc caatgcctaa accgatacac agtaataatg caaggttgcc aaacaccaca
     8461 ctgcctgccg atgccataat ctggaaaatg ccctgtaaag tcgggttatc cagtatcggg
     8521 taggcttgca ccgttgcctt attcgacaaa gctccgccaa tccctaacag caaaccggct
     8581 gccggtaaaa tcgcaatcgg cagcataaaa gcttttccga cttgttgcag ttgtttgaac
     8641 ataaaatttc tcctttttta tggttgtatt tgatggttta tagcatactg aaatttagcc
     8701 gtatgaggtg tgatataact cacagatttg caaaattttt agcaaattcg accgcttgta
     8761 aaaaaattac cgcttgcgga aaaattgaag tagaattaag cattttttta gcaaacacaa
     8821 cataaaaaat ttggagaaga ttatgcctat tttacgttca gcaacttcca ctcagggtcg
     8881 taatatggcg ggggctcgtg cattatggcg tgcaaccggt atgaaagaaa atgattttgg
     8941 taagccgatt attgcagtgg tgaactcatt cacccaattt gtgccggggc acgtccactt
     9001 aaaagatatg gggcagttgg ttgcggcaga aattgaaaaa gccggtgggg tggcaaaaga
     9061 attcaacacc attgcggttg atgacggcat tgcaatggga cacggcggaa tgctttattc
     9121 tttaccaagc cgtgatttaa ttgcagacag tgtggaatat atggtgaatg ctcactgtgc
     9181 tgatgcgatg gtgtgtatct ctaactgcga taaaatcacc ccgggaatgt taatggcagc
     9241 aatgcggtta aatattccga ctatttttgt ctctggtggc ccgatggaag ccggaaaaac
     9301 taaactttcc gatcaattaa ttcgcttgga tttagtcgat gcaatgatcg aaagtgccga
     9361 cccgaatgtg tctgatgaac gtattgatgc gattgaacgt agtgcttgcc cgacttgtgg
     9421 ttcttgttca ggtatgttca ccgcaaactc aatgaactgt ttaactgaag ctttaggctt
     9481 aagtttaccg ggcaatggtt caatgttggc aactcatgct gaccgtaaag agcttttcct
     9541 aagagcaggt cgtgaaattg tggaactttg ccgtaaatat taccaacaag acgatgagag
     9601 cgtgttacct cgctcaatcg ctaccttcga tgcatttgaa aatgcaatga gccttgatat
     9661 tgcaatgggt ggttcaagta ataccgtgct tcatttacta gcgactgctc aagaagccgg
     9721 cgttgatttt aaaatggaag atattgaccg cctctctcgt gtcgtgccgt gcttaagtaa
     9781 aattgccccg aataccaata aataccatat ggaagacgta caccgtgccg gtgggattat
     9841 gggcttgctg ggcgaattag atcgtgcagg tttaatccat cgccacgtta aaacagtgct
     9901 aggtttaacg atagaagagc aactgaatca atacgatatt attcgtaata aagatgaaga
     9961 tttacacaaa ttcttccgtg caggtccggc aggaattcgt actactaagg ctttctcgca
    10021 agattgccgt tgggacactg ttgatgatga ccgtgtaaac ggctgtattc gcaatgtgga
    10081 aaatgctgtg accaaagagg gcgggttagc ggtattattt gggaatattg cagaagatgg
    10141 atgtattgtt aaaaccgcag gggtagatga gtctatttgg aaattcactg gcaccgcaat
    10201 cgtgtttgaa agccaagaag atgcggtagc aggtatttta ggcggaaaag tgaaagaagg
    10261 gcatattgtg attatccgct atgaaggtcc gaaaggcggc ccgggtatgc aagaaatgct
    10321 ttatccaact agttatttga aatcaatggg cttaggcaaa aaatgtgctt tattaactga
    10381 cggtcgtttc tctggcggta cctcagggtt atctatcgga cacgcttcac cggaagccgc
    10441 ttcaggagga gcgattgcct taattaatga tggcgatacg gtggcgattg atattccgaa
    10501 tcgttcgatt aacttaatgg tttcagatga agaacttgct gctcgccgtg cagaaatgga
    10561 agcccgtggc gataaagcgt ggcagccgct aaaacgtgag cgagaagtgt cgtttgcact
    10621 aaaagtgttt ggacattttg ccacctctgc tgataaaggt gcggtgaggg ataaatcgct
    10681 gttaaagtaa ataaattaga aaaaagaagc atttcaataa tatattgatc tgctctttat
    10741 tcagctgaat atttaatcta gctgaatgga gagcagatcg atagcctaat ttacatcttt
    10801 ttgttttgat acagggaaat gtattacaag cattttgccg gttttggcag acctgcaatt
    10861 ttagtggctt gttttgcagg accatcagga aacaagcgtt gtaaataacg gctgttgcct
    10921 ttttcttcac cgaatttttg tgccattgtc tttaccagca ttcgaattgc tggtgaagtt
    10981 ttgtattctt ggtagaaatc ccgcaccaga taaataattt cccaatgttc tgcggttaag
    11041 ctaatttgct ctttttcggc gattttttct gctaattcta cagaccaatc atttaaatta
    11101 gttaaatagc ctgaagcatc ggttgggtat tggatattat ttaattcaat cgtcatcaaa
    11161 taaaaatgcc acataatgtg gcattcctct taaactaaaa cgctagtcgt cactacctag
    11221 cacaccgaag atttgtaaga agcttacaaa taggttgtag atagacacat ataaatcaac
    11281 ggttgcacga atgtagttgg tttcgccacc gtgaataatg ttgctggttg ccagtaaaat
    11341 cgcaccgctt gagaaaatga caaacaagct gcttagggct aaacttaatg ccggaattgc
    11401 taagaagata ttggcaataa taccgactaa taataccaca aacagggcaa ccatcattcc
    11461 gctgataaag ctcatatctt ttttggtggt taatacataa gctgaacaag cgaagaaaac
    11521 caatgcggtt gcaccgaatg caagggcgac aatatcccct aaacccgcac cgatgtaacg
    11581 gtttaaaata ggacctaagg tatagcctaa gaagccggtt aaagcaaata cgcttaagat
    11641 acccgcactg ctgtttgagg tggcattcgt caagaaaagt aagccgtaaa agccgaccaa
    11701 taatccccac cacggcatag ccggtgcgtt catttgaatt gcaacaaaag caacaactgc
    11761 ggagaaagtc agggtcattg ccaataacat ataggtattg cgaagcactt tgtgggtgct
    11821 aattagagat tcaccagttg aatagctgtg agctaaacga ttttccataa aaattccttc
    11881 tgtttttgtg tttaaatcgg aacatccgat ggattaatat ctatggataa atttccataa
    11941 atcaagaagt aaggtagccg taatttattg taaagtcaat gcgattttgt aacagaagag
    12001 taaaaaaaaa catttagttg tgttacactt aataccactc tactttatat tccttttcat
    12061 cgatgaattc gctatgaagc tcaatttccc aacttattta accttatttc gggtaggatt
    12121 aaccccactt tttattgtgg ctttttatct gccgacaaaa tatgcgcctg aaatttcaac
    12181 acttattttt tttattgcat cgattaccga tgcctttgac ggctatttag cacgcaaatg
    12241 gaatcaaacc acccgacttg gtgcattttt agatcctgtt gcggataaag tcttggtagc
    12301 tgttgcgttt gtctgcgtgg cggagtatta ccatacttgg tggattacta ttccaatctg
    12361 cattatgatt gcccgtgaaa ttattatttc ggctttgcgt gaatggatgg cggagcttgg
    12421 ggaaagggcc agtgttgccg tttcgatttg ggggaaagta aaaacgactg ctcaaatgct
    12481 cgcattaggt ggaatgttat ggagacagaa ccaactaatg gagattttag cctttattct
    12541 gctttatatt gcagtgggtt taacgatctg gtctatgatg cagtatttaa atgcttcaaa
    12601 gaatagtcta ttgaagtcat aagcggtcat atttttattc ttttttgcaa tttgtagagt
    12661 ggaaatatcg ctctacaatt ttcaatttta tcaactctaa tgtctgaatt taacgctaaa
    12721 tccttcctgt taaatgtgcc tcaccaaccg ggtgtttatc gtatgtatga tagtaaaggc
    12781 acgataatct atgtgggtaa agccaaagat ttaaaaaagc gtttagccag ctattttcgt
    12841 agccaacttt ccagtaaaaa aacggaagcc ttggtggcaa atcttgctaa tattgaaacg
    12901 acgatcactc attctgaaac agaggcgtta ctccttgaac ataattatat taaggaaaat
    12961 cagccgaaat ataatgtgtt gctcagagat gacaaatctt atccttatat tctgttaact
    13021 aaacataagc atcctcgttt aacagcattt cgtggtagta aaaagatcgc cggtgaatat
    13081 tttgggcctt atccgaatgt tacggcagtt cgggaaacac tgaatttatt gcaaaaaatt
    13141 tttcctattc gtcagtgtga agatacttat tataaaaacc gttcacgacc ttgcttacag
    13201 tatcagatcg gacgctgttt agccccttgt gtagagggct gttattcacc cgttgaatac
    13261 gatcagcagg ttaatttggt tcgtttgttt ttacagggaa aagatcacca agtagtcgag
    13321 catctggtac agaaaatgga agatgcggcg gaggctttgg attttgaagg tgcggcacgt
    13381 tttcgggatc aaattcaatc agtaagagcg gtgcaagaaa aacaattcgt ctctaatgat
    13441 aggctagatg atcttgatat tatctcgatc gtttatcaaa atgggattgc ctgtgtgcat
    13501 atcttatttg ttaggcaagg taaggtacta ggcaatcgtt cttactttcc aaaagtgcca
    13561 agtaatacag atttaaccga gctcgcagat acctttgtcg gtcagtttta tttacaaatg
    13621 aaccagcacc gcaccatacc taaccaaatt gtaattgatc atcaattaag tgaatctaag
    13681 gcactcagtg aaattttaag tgagcaagca gggcataaag tcagcatttt agataatgcc
    13741 atcagaggtg ataaacagcg ttatttagct ttagcgagaa ctaatgcaat ggcagcatta
    13801 gttttacaac ttaagcaaga atcccatatt caaagccgtt atcaggcatt gaaagaaatg
    13861 ttgaaaatgt cagagattaa aagaatggag tgctttgaca tcagtcatac aatgggcaac
    13921 caaacagtgg cttcttgcgt ggtgtttgat gaaaatgggc cgctaaaatc ggattaccgc
    13981 cgttttaaca ttgaggggat taccggaggt gatgattatg cagcaatgga gcaggctctc
    14041 ttgaaacgct ataatcgaga attaccgcca gaaaagatcc ctgatattat ttttattgat
    14101 ggtggcaaag ggcagttgaa ccgagcgtta gatgtcttcg ctaaacttga tgtggaatgg
    14161 gataaatcga aaccgctttt aattggtgtg gcaaaaggcg tagaacgtaa agctgggctg
    14221 gaaactttaa ttatcagcaa atgggataaa gaaattcact taccgccaga tagtcttgcc
    14281 ttgcatttaa ttcagcatat tcgagatgaa tcccaccagc atgccattag tgggcatcgt
    14341 aaaaaacgcc aaaaggcctt taccgaaagt ggattagaat ctattgccgg tgtcggtgca
    14401 aaacgccgac aagcgttgct aaaatattta ggcggaatgc agggggtgaa gtctgcaacc
    14461 ttggaagaga ttcaatcagt tccgggaatt tctaaagcgt tggcagaggt tatttttgat
    14521 acattaaaaa atagttaaat tatcaatagc gatatcgctt ccagccgtaa tggtagccca
    14581 ctcctatgct tgggtaaatt atagggtgaa ttggcagatt tatgctttct gagcgtttat
    14641 cgctttggtt taatggctca tcatgttgct ttgtattttt atcaacagtt tgcttattga
    14701 ttactcgttg atggtaatcc tgaactgctt gcatatccat tggtactgtg ctttgttgtt
    14761 ggggtgtcga acaggcggtt agtgcaacgc tcaatccgag taaaattaat tttttcatat
    14821 catttcctta tattgcaaaa tattttgctt ttttgaccgc ttattttgct attaattctt
    14881 aaaaagatta ttttatccat aaaaaaggac gcactaggcg tcctcatttc tattctttaa
    14941 aattaaagag cagattttgc tttttcaact aaagctgcga aagcaacttt gtcaaatact
    15001 gcgatgtcag ctaagatctt acggtcgatt tcaacagatg cttttttcaa gccgttgatg
    15061 aatttgctgt aagataaacc gttttgacga gctgctgcgt taatacgagc aatccataat
    15121 tgacggaatt gacgtttacg ttgacggcgg tcacggtaag catattgagc agctttaact
    15181 actgcttgga aagcaacgcg atacacgcgt gaacgtgcac cataataacc tttagcagcc
    15241 ttaagaactt tcttatggcg tgctcttgca ataacaccac gttttacacg agccattatt
    15301 taatctccta attgtctatt ttgattggtg gtcgtcatca cgacctttac taagaattct
    15361 atccgtacgt ccgtacttta ctacactacg ctaacctaaa aggcttatgc gtatggtaag
    15421 cacgctacta ctaaaacttg gtcagctttc gctaccattg atttgtgacg taattgacgt
    15481 ttacgtttag tggttttctt agtcaaaatg tgacgtaagt gagattgttt acgtttaaaa
    15541 ccgccggaag ctgttttttt gaaacgctta gcagcaccac gtactgtttt aattttaggc
    15601 attgttaaaa aactccgcat tgtttgttag atattatgaa aataaggcga attttgtgaa
    15661 caaaattact tggaagcact tattctccgc tatacgattt tcatcgttaa aaagtcaagc
    15721 aggctgctga gtttgcctgt tgagcatttt ctctttcttg gctaggcaaa aaagcttgcg
    15781 aattttagcg aaaatataac cgctatgcaa gccaatttaa gctttatcaa gaaaaaagca
    15841 cattaaaaca atgtgcttat cgaaattatt tcttcttagg tgcgatcacc atcacaactt
    15901 ggcgaccttc caatttgcct ggggctgact ctaccattgc aatttctgca gtatctgctt
    15961 tgacacgttc taaaacgtcc ataccgattt cttggtgagc catttcacga ccacggaaac
    16021 gcactgtgat ttttacttta tccccatctt ctaagaaacg gataatgcta cgaagtttaa
    16081 cttgataatc gccatcatca gttcccggac ggaatttgat ctctttaact tgaacaactt
    16141 tttgtttttt cttttgttct tttgctgctt tctctttttc gtagatgaac ttaccatagt
    16201 tcataatacg acaaactggt ggttcagcat ttgggctgat ttccactaaa tccaattcag
    16261 cttcttctgc tctatttaga gcatcttgaa gtgatacaat accaacttgt tcgccgtttt
    16321 catcggttaa gcgaacttct tttacgccac gaatttcgtc attaagacga tgtgcacgat
    16381 ttgattgaac acgttttaca ggtttaatag ccttattcct tctagtaaat ttttgcttaa
    16441 attttaccgc ttgagagcgt ataaatattt tagattgctt gttttaagtg attaaaaaac
    16501 agctaaacgt gcggattcta taagattttc agtgcttatg caataataaa gcccttattt
    16561 aactgcctat tttcggttat tttgatattc tttgattaaa ctcataaatg cttgcccaaa
    16621 tcgttcaaat tttcgttcac caacaccatt aatttctaac atttccactt ttgaagtcgg
    16681 gagaaattct gccatttctt gcaaggtggc atcgttaaag acgatataag gtggaatatt
    16741 ttcgaggtca gctatctgct tgcgtaaaaa acgtaagcgt gcgaataggt ctttgtcata
    16801 gcgatgactt gcttgttttt gcacaaatgc agtcgcagat atacctaaac gtggcaatgc
    16861 aagctgaagt tgcttttctc cacgcaaaaa tggtctggca ctttcggtta attgcagagc
    16921 ggaatggtta gcaatatttt gaataatcaa ccctaaatga atcagttgcc gcaccacatt
    16981 ttgccaataa tgttggcttt gttcttttcc gataccataa acggaaagtt gatcgtgttg
    17041 atgttcacga attttgagat tgttcattcc tcgcaatacc gcaataatat ggtgctgacc
    17101 gaaaatttga ccggtgcggt aaatcaccga catcactttt tgggcatcaa gtaagccatc
    17161 gtattgttgc ggtggatcta aacaaatatc gcagtttttg cattgctctt ggcgatgttc
    17221 cccaaagtag ttgagtaaga ctaaacggcg gcaagtctgt gattcggcga aatcgccaat
    17281 tgcctgtaat ttatgctgtt taatatttct ctgttcgctt tccggctctt ccaataatat
    17341 tttttgtaac caagcataat cgctaggatc ataaaaaagc acggcttcag aagggagatc
    17401 atctcgcccg gctcgtccgg tttcttgata gtaagattcg atagagcgag gtaaatcgaa
    17461 atgtaccaca aaacgtacgt tggatttgtt gatccccata ccaaacgcaa tagtggcaac
    17521 cacaatttga atatcatcac gctgaaaggc attctgcact cgttctctct gttcaaaact
    17581 cataccggcg tgatagccca tcacgctgat tttacgggct gagagtttct cggtgatttc
    17641 ttccactttt ttgcgactgt tgcagtaaac aataccgctt ttgccttgct gtttgctgat
    17701 gaatttaatg agctgttcaa gcggtttaaa tttttcctgc acggtgtagc gaatattggg
    17761 gcgatcaaag ctgcctaaat agatatgtgg gctatttaag cgtaaatgat gcagaatatc
    17821 ggagcgggtt gttagatctg ccgtggcggt gagtgccatc aacggcacat tcgggaaagt
    17881 gtttcgtaag ccacctagca gggtatattc agggcggaaa tcgtgccccc attgggaaac
    17941 acaatgggct tcatcaaccg caataaagct gattttacat agcgaaataa agtggaaaaa
    18001 gccttgagtc attacctttt caggggaaag atagagtaat tttaattgac cggataaggc
    18061 tttttgttcc acaagttgct gctcttcgag cgtttgagtg gagttaagat aagctgcttc
    18121 aatgccgttg gtgataagtt gatccacctg gtctttcatc aacgaaataa gcggcgaaat
    18181 caccaaagtg atgccgtcta ggcacaaagc cggcacttgg tagcaaaggg atttgccacc
    18241 gcctgttgtc ataatcacga ggcaatcacg attttccaac acggcattga taacttcttc
    18301 ttgcccgtct cggaattttt gatagccaaa aatattattt aaaacagaaa ttgcggtggt
    18361 gttcattggt agttggttgc tacttatgat agtactcgtc ttctgagagt atcttttaaa
    18421 atgaaaataa agcactcatc aaagacgagt gctgtcttag ttaacaatta ggcattagaa
    18481 agaaacgctt tctccgtgtc gagcaaaagc ttcttctaaa tgttgccaga acgagcgacc
    18541 ttcatctgtt acccattcgc cttctacatt tttaaagtgg aaaccaccca atttgctggc
    18601 aagccataat tccagcattg cctcttgttt attaatgata acttgcgatt catcatcaaa
    18661 ggttaaggtg cagaccgctc cttggatttc agtatcgact gataatcctt cgtcataaat
    18721 tttttcttca atttgttgcc atacttgctc tacggcttgg tggaattgtg ctgtgttcat
    18781 tggtgtatcc ttatttgaat gttatataag tagtaaatac tttttgtttt agctgtttaa
    18841 atggtctgta aagttctttt tgtatttttg ttaagaaata attaggcttt ttaagtttag
    18901 cttgattttt tattttatcc tcattatta
//
LOCUS       DANLXS010000028        18758 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.028, whole genome shotgun sequence.
ACCESSION   DANLXS010000028 DANLXS010000000
VERSION     DANLXS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18758
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.028"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(249..734)
                     /locus_tag="PXD71_000760"
     CDS             complement(249..734)
                     /locus_tag="PXD71_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HDL6290912.1"
                     /translation="MNLISDEIYRQLVKDSGLNTSLKQLFSHFDTGSDYELLQEQFTQ
                     ARAYFMAAQDSMVQSVRQDLSPLAVYMIKDKASSSGGTFLRWRSMQNARTGGTVWQPI
                     VDDKSVPVEVRKKVVAVEKDRILINMQISVFNHILRQLADCAEKLKEVDNAVAKSDLN
                     S"
     gene            complement(743..1501)
                     /locus_tag="PXD71_000761"
     CDS             complement(743..1501)
                     /locus_tag="PXD71_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HDL6290913.1"
                     /translation="MTTQLDPQLGPLRSEITFTLHTQYAHKLWVGRPMIRNEEGKVTQ
                     SSIISVPNCFAMLTQIQRAASEDDPYADDYLIQFEESVINYRKEIQKLTNDIVTLYAK
                     MLPEGISIERCANISPISYPIYVNSQLGYQLLYLLGDFDSLCRSVMTAAHIAIINRAE
                     AQDWIEAGARLIRKCFGIVERYKSSGITRRDYQEKNARYQAAIKRMGYTLSDAVLTGE
                     HRAEFAPFIKQNTTVEEEQSVETHITAQTNESQK"
     gene            complement(1680..2870)
                     /locus_tag="PXD71_000762"
     CDS             complement(1680..2870)
                     /locus_tag="PXD71_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290914.1"
                     /translation="MFSSTNKPTEHGLLFFGNQHETVPTRLLQDPCLTPRAKFAWQLI
                     KCNVQAFQGGLFPSYETLSKMLSDRPYAEAKLSEKVVTQTLYLLRLTRWLTLCETVRD
                     ERGYVQGNVYILHDEPVPILDAIQLNSDYLSFLDKCTKHKDSLINGVANHIVESLITD
                     KTKWHYVSHLDCLQSRYQIYQQQQLVKDKQPSQTEQELSKIQQNILTSTMEVRDFDEE
                     VRKKSSKNQTSNKGVRAKNEDKSLILDLLPLGKSDVQYRTLYINKYCTGEPSEITWPQ
                     EIPLTSLEQKLTAQAMKGLDLGLCQAILLEVQQRIIQKQDVKKPKGYLYTLIQRAKQG
                     EFKPYYFELGKNLPSQNQANNNSAHSIQKLTEQPTEKPVLTSADREQMASRMQKFRAE
                     LLLQ"
     gene            complement(3029..3586)
                     /locus_tag="PXD71_000763"
     CDS             complement(3029..3586)
                     /locus_tag="PXD71_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDL6290915.1"
                     /translation="MIKPITVNEALIGNALLNIKEGNIHPCLKMGFSEEQLKAINNLS
                     IDEIMDISNSVVSFAKVEINHETFWKLLAIAQANTQQRQIIDRALLLGASIEMLHQYF
                     GLSTSEVSARRQLLGIEEKMGRKAAASDEESTHIWEIWQRYKQRMEILDSQEGLELLC
                     LIAEEGNMNLTVVWKLVSEWYSKHK"
     gene            complement(3579..5225)
                     /locus_tag="PXD71_000764"
     CDS             complement(3579..5225)
                     /locus_tag="PXD71_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL6290916.1"
                     /translation="MTKDEKRLAAIAKGLNVSPISNTAPSYQTATPTHYSSDVEYITV
                     TLDKLRPYEHNPRKTRNPNFEMIKESIRRRGLDHKPNITRRPGEDFYIIADGGNTRIQ
                     ALKELFTETKDPKFWSISCQYKPWQGDTADSVEAELNLLIGHLIENDTRADLSFIEKA
                     LGILQAKEYYEKKLGKSLSSRELSSELENDGYIISHALILKMERCVNYLYPHIPEVLF
                     SGLGHTQIDKLLAIRNNAEEVWAAHQFDTDVTFESLWSENLSKFNEATPFQAKEFQSE
                     LITAMVEALEGKVSFETLYLDIDLDEQKFKKIAAKQQEIDNLVEKTVDQINTRQEQQT
                     AKSEPLIKNENKAVTPQKAQHIIDEVIDDNESDDEQNNAITDLSQLGSSLLDNQDNQE
                     DYLSQIAHNFGLTPGMSIQEQREQRAEANGLTFACEGRQPVEDIWLIHPARQYRSEAY
                     SLALDIAESMGIDHLVEHIVKQPVDYTYAMKPADTELSPSALTLYSFLSMLQTAEHKD
                     ENSCVLDSKFLFELDDLTLVKIFRLIRVSRHIRQQGGEND"
     gene            complement(5234..6595)
                     /gene="dnaB"
                     /locus_tag="PXD71_000765"
     CDS             complement(5234..6595)
                     /gene="dnaB"
                     /locus_tag="PXD71_000765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL6290917.1"
                     /translation="MSSLNKSLVQPHSIEAEFSVIGGLLLDNELFDEVSSKINEGDFY
                     NFTHRLLFKHISELIEAGKPADILTLDQYLTQKKVIEDLGGFAYLAEIVKNTPSTANI
                     HAYTDIVLLYSKQRQLLKLGQFIVDQTQSAKTPDKFEAVVDDIEKKITEFSLSDNTKS
                     AADLSDIFANMLSRMELSAKNGDPVTGTPTGIQGIDEATTGGQPGDLIVIGARPSMGK
                     TAFSQTIAYHTLKKFESAPVFYHSMEMPADQILQRFLAMRARVGLQNIRQADSLDDEE
                     WSKLTSAMGYILEHWKNRLVIDDEGSLTPQKLRTKVRQNARKYGKPAAIFIDYIQLMQ
                     TSQRYENRNLEIANISRSLKLLAKEMDCPVYALSQLNRSVEQRANKRPINSDLRESGS
                     LEQDADVILFIYRDEVYDADSPFKGTAEIIIGKQRNGPLTSVMTNFRSEFALFENRTN
                     TTH"
     gene            complement(6607..7431)
                     /locus_tag="PXD71_000766"
     CDS             complement(6607..7431)
                     /locus_tag="PXD71_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL6290918.1"
                     /translation="MKPLQNGTNKPFTITIASTKGGSAKSTNAANIGAFCAEHGLRTL
                     LIDTDTQPTLSSYYELEYQAPGGTYEFLHFKDVDPTHIISRTTIPNLDLIQSNDPSNK
                     ISPMLRDSPDGALRFSLLLQKIEGYDVIIIDTRGTRDITVDMSVLAADVLFCPILPHI
                     LSAKEFIRGTIGMYQDLETFTAFGFTLPPLKAMINCVDHTKDVKLVSDYLHTLFQNEF
                     DENKTLLEFSVPDRVAYREAATFSKPVYRQSQAEYETISQLCALLMPQFANSHFSA"
     gene            complement(7529..7614)
                     /locus_tag="PXD71_000767"
     tRNA            complement(7529..7614)
                     /locus_tag="PXD71_000767"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7578..7580),aa:Leu,seq:caa)
     gene            complement(7926..9386)
                     /gene="xseA"
                     /locus_tag="PXD71_000768"
     CDS             complement(7926..9386)
                     /gene="xseA"
                     /locus_tag="PXD71_000768"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL6290919.1"
                     /translation="MSNILTVTQLNYSVRHLLEMEMSHIWLTGEISNFSQPVSGHWYL
                     TLKDDKAQVRCAMFKMKNVRVNFRPQNGMQVLVRASVTLYEPRGDYQLIIESMQPAGE
                     GLLQQQFEQLKAKLSAQGLFAQEHKKPIPTFVKTVGIITSSSGAALQDILHILQRRDP
                     SLSVVIYPALVQGKEATQDIVNMIELANRRNECDVLIVGRGGGSLEDLWCFNEESVAY
                     AIYHSQIPIISAVGHETDVTIADFVADLRAPTPSAAAELVSRDQNELKRQLQHQFDKV
                     NLAFDRVWNHKFNRFQQLPLRLNAQHPSKQIQSQQQRFDYMEYRLEQAIYALVAAKKQ
                     VGKELNVRLIHQHPQKQIERQSQMLKQLELKLHWQMEQQLSKKQQKWKNITQRFERNP
                     LPYQLYQQQTMVAKMSQRLAYSIEKIMLKEQRHFQELCTKLDGLSPLKILTRGYSITQ
                     TKDGKTLTSTENIERGEAIITQLKTGKIISQVVDFL"
     gene            complement(9537..9613)
                     /locus_tag="PXD71_000769"
     tRNA            complement(9537..9613)
                     /locus_tag="PXD71_000769"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9577..9579),aa:Met,seq:cat)
     gene            9823..10929
                     /gene="mltA"
                     /locus_tag="PXD71_000770"
     CDS             9823..10929
                     /gene="mltA"
                     /locus_tag="PXD71_000770"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDL6290920.1"
                     /translation="MNWKAYKKAIAAASVVAIATVLVACSSSGQKGKTTSYDAEKAKF
                     GAKYNGRHFMPYGTHFQAGRLGLNAAPVNQLDFLQQLSNVRAYSSLADSYAIAYGKIS
                     RWISSGGNINDLAKYGISTRLMRGEDGYQNVLMTGYYSPVIKARRTPQGQYQHPIYAM
                     PSSKRFSRAEIYAGALQGQGLELAYSNSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQ
                     NGFKYASVGRLLVEDGEIPKEKMSVQAIREWGQRNPSRVQGLLERNPSYVFFKNDPTG
                     QVKGSAGVPLVPLASVASDRSIVPSGSVLLVEMPLIDSKGNWTGKHEMRLMVALDVGG
                     AVKGHHFDLYQGIGDKAGHIAGLMKHYGRVWVLN"
     gene            10942..11391
                     /locus_tag="PXD71_000771"
     CDS             10942..11391
                     /locus_tag="PXD71_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290921.1"
                     /translation="MLKKLSAIFLLLPSAVLANNLQNITASTEPKYKISEIDVRILIR
                     QLNNIEQCIYPELAKPGYQQIYANWNLAENLTMQYFEYQLLKELLGEENQKLMQNDTL
                     STEYFHLLHSRLNHQKANVDPEKCDTFKPRYKEIYKSMENALTKKNQ"
     gene            complement(11425..12942)
                     /gene="purF"
                     /locus_tag="PXD71_000772"
     CDS             complement(11425..12942)
                     /gene="purF"
                     /locus_tag="PXD71_000772"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL6290922.1"
                     /translation="MCGIVGIIGNSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFR
                     LRKANGLVSDVFQQEHMVRLQGNVGIGHVRYPTAGSSSVSEAQPFYVNSPFGITLVHN
                     GNLTNNAELKARLYNEARRHVNTNSDSESLLNIFAYFLDLYSTQHLSPDNIFETVRKT
                     NDSIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKREIEGKTEYMFASESVALDVVGF
                     EFVRDVLPGEAIYVTFDGQLHSQICADNPKLNPCIFEYVYFARPDSVIDGVSVYSARV
                     HMGELLGEKIKREWGRIIDDIDVVIPIPETSNDIAVRIANMLYKPYRQGFVKNRYVAR
                     TFIMPGQAQRKSSVRRKLNAIASEFKGKSVLLVDDSIVRGTTSEQIVEMARAAGAKRV
                     YFASAAPEIRYPNVYGIDMPTCEELVAYDRSVEEVAQMIGVDKLIFQDLEALYKSIQL
                     ENPTIHRFDDSVFTGEYITGDVDKCYLDSIARSRNDKAKAEAAKQATNLEIHNER"
     gene            complement(12957..13460)
                     /locus_tag="PXD71_000773"
     CDS             complement(12957..13460)
                     /locus_tag="PXD71_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL6290923.1"
                     /translation="MVDLIVIGIIAFSVLISLWRGFTNEALSLLGWVIAFFVASSFYP
                     YLSGYLTQINSVYLQNSEFLRNGIAAAILFILTLIVCGVINGLLGKVIDLSGMSITDR
                     ILGGAFGALRGILIVSAILFFLDTFTQASQTELWKSSLLIPHFDFIVKWFFEQLQANS
                     SFLNTTK"
     gene            complement(13606..14067)
                     /gene="rnhA"
                     /locus_tag="PXD71_000774"
     CDS             complement(13606..14067)
                     /gene="rnhA"
                     /locus_tag="PXD71_000774"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDL6290924.1"
                     /translation="MKLVEIFTDGSCLGNPGKGGIGILLRYNGYEKTVSKGYFQTTNN
                     RMELRAVIEALAMLKEPCKVQLNSDSQYMKNGIQKWIFNWKKNDWKTSDKKPVKNKDL
                     WVALDQEIQRHQIEWSWVKGHSGHRENEICDELAKQGANNPTLDDVGYIAD"
     gene            complement(14160..15491)
                     /gene="argG"
                     /locus_tag="PXD71_000775"
     CDS             complement(14160..15491)
                     /gene="argG"
                     /locus_tag="PXD71_000775"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005722481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDL6290925.1"
                     /translation="MSATILEALPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTA
                     NLGQPDEDDYNAIPKKAMEYGAENARLIDCRQQLAHEGIAAIQCGAFHISTGGIPYFN
                     TTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
                     QLFIDELGGRLEMSKFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIV
                     KPIMGVAFWDESVEIKAETVSVTFEEGVPVALNGKRIDDAVELIMEANRIGGRHGLGM
                     SDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEK
                     VEDAPFTPADRIGQLTMRNLDIVDTRGKLAVYTDAGLLAANNSVIPQLGKK"
     gene            15675..16715
                     /locus_tag="PXD71_000776"
     CDS             15675..16715
                     /locus_tag="PXD71_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL6290926.1"
                     /translation="MKKLLVIRNDKIGDFMVCFPAFAMLKQSMPNVEITALVPSYTAP
                     LAELCPSIDKIIIDTANKKDKNEFNRILQAVKNEQFDAVICFVSDWHNAKLTWQSGIK
                     YRLAPATKLFQFLYNHRLTQRRSQSAKAESEYNLDLARAFLSKHNIPIVEPHTPYLSV
                     EQQTVQAQKAKLCEQLNITTVKKWVFVHSSTGGSANNLSIEQYAELIQGILAEFDCYV
                     VLTAGKGESEKAQQLAQQINHNNVVVYDKNEGLQDFTHSLACADLFIAGSTGPLHISG
                     ALNIPTIGFYPSRLSAIPRRWKPINDANKHIAFCPPSDKASQMNLTLISISEALKTIV
                     PFIRRMWNTPSL"
     gene            complement(16780..17538)
                     /locus_tag="PXD71_000777"
     CDS             complement(16780..17538)
                     /locus_tag="PXD71_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL6290927.1"
                     /translation="MPTLSVAMIVKNEAQDLRACLDTVKDWVDEIVILDSGSTDETPQ
                     IAAEYNARFYTNTDWQGFGKQRQLAQQYVTSDYVLWLDADERVTAELKQSILQAVKND
                     EKNTVYKISRLSEVFGKQIRHSGWYPDCVVRLYRTEFAKYGDELVHEKVHYPKEAKIQ
                     KLQGDLLHFTYKDIHHYLVKSASYAKAWAIQRANAGKTASLFDGVTHAIGCFVKMYLL
                     KAGFLDGKQGFLLAVLSAHSTFVKYADLWNRTRK"
     gene            17665..17979
                     /gene="yhbY"
                     /locus_tag="PXD71_000778"
     CDS             17665..17979
                     /gene="yhbY"
                     /locus_tag="PXD71_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL6290928.1"
                     /translation="MSITLTTKQKQFLKGLAHHLSPVVMLGGNGLTEGVLAEIDNALD
                     HHELIKVKVAGADRETKQLIIDAILRETKAAEVQTIGHILVLYRQSEEKKISLPKATK
                     AI"
     gene            18040..>18758
                     /locus_tag="PXD71_000779"
     CDS             18040..>18758
                     /locus_tag="PXD71_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL6290929.1"
                     /translation="MFKKFSLYTLAMLLIPVVTWLANWHWSLAIQYAEFDFDYFLYLI
                     TETGSVPYAIITCVVFALLLARVVRKRYSLIFVITVCVASMVATQAIKTAMKSTFQEP
                     RPFVTALFQDKTDDFYQLTREQRGLAVMEKIGANQNFVTAHQAKEVGYSFPSGHTIFA
                     VSWLLLVVGFCRNMRGQAVVLAHFFAISWAALMLISRLRLGMHYPIDLFTSSLIAFLI
                     NLALFVWFVPWLESKKWFAKKP"
BASE COUNT         5419 a         3868 c         3628 g         5843 t
ORIGIN      
        1 gtgcaatcac ttattcctcc gttgtaaagg ctttttcttc gtgaaggtaa ggggcttgta
       61 cccttacctt taaaaggcct tttaatgcag gatttacaaa ctgcactttt tcagggatta
      121 caaaccggat tttcaggtat tacaaacttg aatatgtcct aattaacgga ctgtttgttt
      181 taccacgtca gcccaccgtg gttggcgttt acacacaacc aatataggag ttgttatata
      241 taaatttact acgaatttaa gtctgatttc gcaacggcat tatctacctc tttcaacttt
      301 tcagcacaat ctgccaactg tcgcaaaata tgattaaaca ccgaaatctg catattaatc
      361 aaaatcctgt ctttttctac tgcgacaact tttttcctca cctccactgg gacagactta
      421 tcatctacaa tcggctgcca aactgtgcca ccggttctgg cattttgcat actacgccag
      481 cgtaaaaatg taccgcctga ggatgaggcc ttatccttaa tcatataaac agccaaaggt
      541 gaaagatcct gccgcacaga ctgcaccata ctatcctgtg cagccataaa ataagcccga
      601 gcttgagtga actgttcttg caaaagttca tagtctgagc cagtgtcaaa atggctaaat
      661 agctgtttca gagaagtatt cagcccagaa tctttgacta attgccgata aatttcatca
      721 gagatgaggt tcatatcact ccttattttt gagattcatt tgtctgtgcc gtgatatggg
      781 tttctacgga ttgctcttct tcaacagtag tgttttgctt gatgaaggga gcaaactcag
      841 cacgatgctc accggtaagt acagcatctg agagtgtata gcccattcgt tttatcgcag
      901 cttgatagcg agcattcttt tcttgatagt cccgacgagt gattcctgaa ctcttataac
      961 gctcaacgat cccaaaacat ttccggatta accttgcacc agcttcaatc caatcttgag
     1021 cttctgcacg attgataatc gcaatatgag ctgccgtcat tactgaacgg caaaggctat
     1081 caaaatcacc aagtaagtag agtaattgat accctagctg tgaattaaca taaatcgggt
     1141 aagaaatcgg ggaaatattg gcacagcgtt caatagaaat tccctccggc aacattttgg
     1201 cgtagagcgt gacaatgtcg ttggtgagct tttggatttc cttccgataa ttgatcacac
     1261 tttcttcaaa ttgaatcaga taatcgtcag cataaggatc atcttctgat gcagcccgtt
     1321 gaatttgggt tagcattgca aagcaatttg gtacgctgat aatggaggat tgagttactt
     1381 tgccttcctc attacgaatc attggtcgcc ccacccataa tttatgggca tattgggtat
     1441 gtaacgtaaa agtgatctca ctacgaagtg gaccaagttg aggatctaat tgagtggtca
     1501 tatatgctcc ttgtttattt gattattttt tagtctttgg aaaggggctt tccattgtct
     1561 gttttttaat ctttgcagac actgcctacg ctgtttattt tttatccatt gtgtacctgg
     1621 tacacactga ttatttttca ctcactatta agccccttaa cagtgattgt tttttaatct
     1681 tattgtaaaa gtaattcagc tctaaacttc tgcatacgac tagccatttg ctctctgtct
     1741 gctgaagtta agaccggttt ctcagtaggc tgctcagtca gtttttgtat agaatgagca
     1801 gaattattgt tagcctgatt ctgtgaggga agattttttc ctaattcaaa atagtagggt
     1861 ttaaattcac cctgcttcgc ccgctggata agcgtataga gataaccttt cggttttttc
     1921 acatcctgct tttgaataat gcgttgttgt acttccaaca aaatagcttg gcataaaccg
     1981 aggtctaatc ccttcattgc ctgtgcagta agtttttgtt caagtgaagt taatgggatt
     2041 tcttgtggcc aagtaatttc gctcggttcc ccagtacagt acttattaat atataaagta
     2101 cggtactgta catctgactt ccctaatgga agtaagtcta aaatcaatga cttatcttca
     2161 tttttagccc taactccctt attggaagtc tggtttttag aggatttttt cctgacttcc
     2221 tcatcaaaat ccctaacttc catagtggaa gttaggatgt tttgttgaat tttgcttaat
     2281 tcttgttctg tttgcgatgg ctgtttatct ttcaccagct gctgttgctg gtatatctga
     2341 taacgacttt gtaaacagtc taaatgagag acatagtgcc atttggtttt gtctgttatt
     2401 agactttcaa caatatgatt tgccacacca ttaataaggc tatctttatg tttggtgcat
     2461 ttatctaaaa aagagagata gtcgctattt aattgaattg catctaaaat tggcacaggt
     2521 tcatcatgta ggatataaac attaccttgt acataacctc gctcatcacg cactgtttca
     2581 cataaagtaa gccagcgagt aagacggagt aaatagagtg tttgagtcac gactttttcc
     2641 gataatttag cctccgcata aggtctatcg gataacattt tgctgagcgt ctcataagag
     2701 ggaaataatc ctccttgaaa ggcttgaaca ttgcacttaa tgagctgcca agcaaacttt
     2761 gctcttggtg taaggcaggg atcttgtaat aatcgagtag ggactgtttc gtgttggttg
     2821 ccaaaaaata gcaatccatg ctctgttggc ttattcgtgc tgctaaacat tgtttctctc
     2881 caccaggata aaagagtttt tctcttgcta ttctctctcc tatggctata atgcacggga
     2941 cttctgcaaa gtccttgtaa ttcataagat agagatacaa gactcaatat cgaatgcccc
     3001 aaagctccaa cttcggggca tttatttttt acttatgttt tgaataccat tcggaaacca
     3061 gtttccaaac aaccgttaga ttcatattgc cttcctcagc aatcagacat agaagctcta
     3121 atccttcttg tgaatctagg atttccattc gttgtttgta tctttgccaa atttcccaaa
     3181 tatgggtaga ttcttcatca cttgctgccg cttttcgtcc cattttttct tcgataccga
     3241 gtaattgacg gcgagccgag acttcagagg ttgataaacc aaaatactgg tgcaacattt
     3301 caatagaggc tccgagcaat aatgctcggt caataatttg tcgctgttga gtattggctt
     3361 gtgcaatagc cagtaatttc caaaaggttt cgtgattaat ctccacctta gcaaaagaga
     3421 caacggagtt gctaatatcc ataatttcat caatactgag attattgatc gcttttagtt
     3481 gctcctcaga aaatcccatt tttagacaag gatggatatt tccctcttta atattgagca
     3541 gggcgttacc aatcaatgct tcatttacgg taatcggctt aatcattttc tcctccttgt
     3601 tggcgaatat ggcgactgac ccgaatgaga cggaaaattt tgactaaggt aagatcatca
     3661 agctcaaaca ggaatttgga atctaagaca cagctatttt catctttatg ttcagcagtc
     3721 tgtaacattg agaggaatga gtagagtgtt aaagcagacg gtgaaagttc agtatctgcc
     3781 ggtttcattg cataagtgta atccactggt tgtttcacaa tatgttcaac cagatggtca
     3841 atgcccatac tttcggcaat atctaaggcc agtgagtagg cctctgagcg atattgacgt
     3901 gcggggtgaa tcaaccaaat atcttcaacc ggttggcgac cttcacaagc aaaagtcagt
     3961 ccattcgctt cagctcgttg ctctcgctgt tcttgaatac tcattcccgg agtgagtcca
     4021 aagttatggg caatttggga tagataatct tcttgattat cttggttatc gagcaatgaa
     4081 gagccaagct gagataaatc agtaatagcg ttattctgct catcatcact ttcgttatca
     4141 tcaattacct catcaataat atgttgagct ttttggggag taacggcttt attttcattt
     4201 ttgatgagag gttcagattt tgcagtttgt tgttcctgtc tggtattgat ttgatcgact
     4261 gttttttcaa ctaaattatc aatttcttgt tgtttagccg ctattttttt gaatttttgt
     4321 tcatctaagt caatatccag ataaagtgtt tcaaaactca ctttaccctc taaagcttca
     4381 accattgccg taatcaattc actttggaat tcttttgctt gaaatggtgt agcctcatta
     4441 aatttggata aattttctga ccagagcgat tcaaaagtaa catcagtatc aaattgatgg
     4501 gctgcccata cctcttcagc attattacga atagctaata gtttatcgat ctgtgtatga
     4561 ccaagaccac taaaaagaac ttctggtata tgtggatata agtaattaac acaacgttcc
     4621 attttaagaa tgagagcatg agagataata tatccatcat tttctaactc agaagataac
     4681 tctctcgaag aaagagactt acctaatttt ttctcgtaat actccttcgc ctgcaaaatc
     4741 cccaatgctt tttcaataaa tgacaaatcc gcacgggtat cattttcaat caaatgcccg
     4801 ataagtaaat tgagttcagc ttcgacactg tcggcagtat caccttgcca aggtttatat
     4861 tgacaactga tggaccaaaa ttttggatct ttggtctcgg taaagagctc cttgagtgcc
     4921 tgaatacgag tattaccacc gtcagcgata atatagaaat cttctcccgg acgacgggtg
     4981 atattcggct tatgatctaa ccctcgacga cgaatagact ctttgatcat ctcaaagtta
     5041 gggttacggg ttttccgagg gttatgctca tacggacgca acttatccag cgttactgta
     5101 atatattcaa catcgctact gtaatgcgtt ggtgtggctg tttggtagga tggagccgta
     5161 tttgaaatag gtgaaacatt taatcctttt gctatcgctg ccagtctttt ttcatctttt
     5221 gtcataatct atcctaatgc gttgtgtttg ttctgttttc aaatagggca aattcacttc
     5281 tgaaattcgt cattacggaa gtaagtggtc cgtttctttg cttgccgata atgatttcag
     5341 cagtgccttt aaacggagaa tccgcatcat aaacttcatc tcggtagatg aataaaatga
     5401 catcggcatc ttgctcaagt gagccggatt cccgtaaatc agagttgatc gggcgtttat
     5461 tggcacgttg ttccacagac cggttcagct gagagagggc ataaaccgga caatccatct
     5521 cttttgccag taatttgagt gaacgagaga tgttggcaat ttctaaattg cgattttcat
     5581 aacgttgcga ggtttgcatt aactgaatgt aatcaatgaa aatagctgcc ggtttgccat
     5641 atttacgagc attttgtcga actttagtgc gtaatttttg aggcgtgaga ctaccttcat
     5701 catcaataac taaacgattt ttccaatgtt ctaggatata gcccattgct gaggttagtt
     5761 ttgaccattc ctcatcatcc aaactgtctg cctgtctaat attttgcaat cccactctgg
     5821 cacgcatagc tagaaaacgt tgtaaaatct gatctgccgg catttccatg ctgtgataga
     5881 acacaggagc cgactcgaat tttttaagtg tatggtaagc gatagtttga gaaaatgcgg
     5941 tttttcccat agaagggcgg gcaccaatca cgattaaatc gcctggctgt ccacctgttg
     6001 tcgcttcgtc aataccttga ataccagttg gagtacctgt cacaggatct ccatttttag
     6061 ccgagagttc catacgagag agcatattgg caaaaatatc acttaaatct gctgcagatt
     6121 ttgtattatc tgataggcta aactcagtaa tttttttctc aatgtcatca acaacagctt
     6181 caaacttatc tggcgttttc gcactctgtg tctgatcaac aataaactgc cccagtttta
     6241 aaagctgtcg ctgtttacta tataagagaa cgatatccgt ataagcgtgg atattagctg
     6301 tagatggagt gtttttgact atttctgcaa gataagcaaa tccgcccaaa tcctcgatca
     6361 cttttttctg ggtaaggtat tgatccaatg tcaatatatc tgctggttta ccggcttcaa
     6421 tcaattcgga aatatgctta aacagtagac ggtgagtaaa attataaaaa tccccctcat
     6481 tgattttgct actcacttca tcaaagagtt cattatccaa gagtaatcca ccgattactg
     6541 aaaattcagc ctctatagaa tgcggctgta caagtgattt atttaagcta gacatacatt
     6601 ctccctctat gctgagaagt gagagtttgc aaactgtggc atcagtaacg cacaaagctg
     6661 actgatcgtt tcatattcag cttggctctg acgatagacc ggttttgaaa aagttgccgc
     6721 ttcacgatat gccacgcggt caggtaccga aaattccagc aatgttttgt tttcatcaaa
     6781 ctcattttgg aagagagtat gaaggtagtc ggaaaccaat ttgacatctt tagtgtgatc
     6841 aacacagtta atcatcgctt tcagcggtgg tagggtaaag ccaaatgccg taaaggtttc
     6901 gaggtcttga tacattccaa tcgtgccacg aataaactcc ttagcagata aaatgtgggg
     6961 aagaatagga cagaataaaa catctgctgc caatacagac atatcgacag taatatctct
     7021 ggtaccacga gtgtcaataa tgatgacatc gtaaccttca attttctgga gcagtaaact
     7081 aaaacgtaat gcaccgtcag gtgaatctct cagcattggg ctgattttat ttgacgggtc
     7141 attagactgg atcaagtcaa gatttgggat agtcgttcta gaaataatgt gggtaggatc
     7201 tacatcttta aaatgcagaa attcgtaagt tccaccggga gcttgatatt ccaactcata
     7261 ataagagctt aatgtgggtt gggtatcggt atcaataaga agggttctta gaccgtgttc
     7321 tgcacagaat gcaccaatgt tagcagcatt cgtactcttg gcagagccac ctttagttga
     7381 agcaatagta atggtaaaag gtttgtttgt accgttttgt aatggtttca taatttactc
     7441 tcttaacaag ggttaaggag tattggccat taatacggtg tatttgaatt gattatttga
     7501 aagagagaaa ctacaagtgt gattcaaatg gtgcctgagg tcggactcga accgacacgg
     7561 ttattcacca acggattttg aatccgtcat gtctaccaat ttcatcactc aggctaagag
     7621 tgctttttat tcaatcgcct gtatgtccta tcagtttacg cataagattt tgaatccgct
     7681 gcgtctaccg atttcgccac actggcttaa tttttgcacg attataggga aatatgaccg
     7741 cttgtgcaag cgtaaaaatg ccttttgaaa tcggttgatt taaaaatcag cactcaattc
     7801 atctttacaa aaatagttat gcacatcacc tgtggataac taagtgaaca accctgttta
     7861 taaatattta acctactgat tttactataa ataattttaa aaattggcag cgttcaaaat
     7921 ttcccctaca aaaaatccac tacttgactt attatttttc ctgtttttag ttgggtgata
     7981 atagcctcac cacgttcaat attttctgtt gaagtaagcg ttttaccgtc tttagtttgg
     8041 gtaattgagt agcctctggt caagattttc aatgggctaa gtccatcaag tttagtgcat
     8101 aactcttgaa aatgtcgctg ctctttcagc attatttttt cgatagaata agccaagcgt
     8161 tgcgacattt ttgccaccat cgtttgttgc tgataaagtt gataaggtag cgggttgcgt
     8221 tcaaaccgtt gcgtaatgtt tttccatttt tgctgttttt tgctgagttg ctgctccatt
     8281 tgccaatgga gtttcagctc caattgctta agcatttggg attggcgttc aatctgcttt
     8341 tgcggatgtt ggtgaattaa acggacattt agctctttcc ccacctgctt tttggctgca
     8401 acaagtgcat aaattgcctg ctcgaggcgg tattccatat aatcaaaacg ttgctgttgc
     8461 gattgaattt gtttgcttgg atgctgagca ttcaggcgta aggggagttg ttggaaacgg
     8521 ttaaatttat ggttccaaac tcggtcaaag gctaaattca ctttatcaaa ctgatgttgt
     8581 aattggcgtt tcaattcatt ttgatctctg ctgactaatt ccgcagcagc cgaaggcgtt
     8641 ggtgcgcgca aatctgcaac aaaatcagca atggtcacat cggtttcgtg tcccaccgca
     8701 ctgataatcg ggatttgtga atgataaatg gcataagcga cactctcttc gttgaaacac
     8761 cataaatcct ctaatgaacc gccacctcgc ccgacaatta atacatcaca ctcattgcgg
     8821 cggtttgcca gttcaatcat attcacaata tcttgcgttg cttctttgcc ttgcacgaga
     8881 gccgggtaaa taaccactga taagctcgga tctcgtcgtt gcaaaatatg taaaatatct
     8941 tgtaatgcgg ctccgcttga ggaggtaata ataccaacgg ttttaacaaa agtaggaatt
     9001 ggctttttat gctcttgagc aaacaagcct tgagctgaaa gttttgcttt aagttgctca
     9061 aattgctgct gtaacaagcc ttcgcccgcc ggttgcatac tctcaataat gagctgatag
     9121 tcacctcgtg gctcatagag tgtaacactt gcccgcacca gcacctgcat accgttttgt
     9181 gggcgaaaat tcactcttac atttttcatt ttaaacatcg cacaacgcac ttgtgctttg
     9241 tcatctttta aagtgagata ccaatgcccc gaaactggtt ggctgaaatt tgagatttcg
     9301 ccggttagcc aaatatggct catttccatt tcgagcaaat ggcggacgga atagttgagt
     9361 tgggtaacag ttaaaatatt gctcataata atcaaaaaag cgtaaatgta gtagaagtgt
     9421 aacgagaaat gaaagcgaga ggaagctttt gcagtcaaca agcggttatt ttttgctgtt
     9481 ttttggcaaa taaaacgaaa accctcgatg tgattcacta tcgagggttt ggaatgtggt
     9541 tgcgggggcc ggatttgaac cagcgacctt cgggttatga gcccgacgag ctaccaagct
     9601 gctccacccc gcgtctgatg aggctataat atatcaattt gacataatga caagtaaaat
     9661 catacaaaat tgctctaagt gatgtaaatt acaacagata tagtatcaaa atacgcttat
     9721 tgttgtaaaa acaatcaaat tatattctgg ttttattttg tcgctgtttt tgctattctt
     9781 ctgtttattt ttcacatttt attaacgata taaggatatt cgatgaattg gaaagcatat
     9841 aaaaaagcta ttgcggcagc tagcgtggta gcgattgcaa cggtattagt ggcgtgttcg
     9901 tcttcaggcc aaaaaggcaa aacaacctct tatgatgcgg aaaaagctaa atttggagca
     9961 aaatataatg gtcgtcactt tatgccttat ggcacacatt ttcaagctgg acgtttagga
    10021 ttaaatgctg ctcctgttaa tcaactagac tttttacagc agctttctaa tgtcagagcc
    10081 tatagtagct tggcagattc ttatgctatc gcttacggaa aaatcagtcg ttggatctca
    10141 tcgggcggta atattaatga tttagcaaaa tatggtatta gtactcgttt aatgcgtggc
    10201 gaagatggct accagaatgt gttaatgaca gggtattact cgccggttat caaagcccgt
    10261 agaacgcctc aggggcaata ccaacacccg atttatgcga tgccgtctag caaacgtttt
    10321 tcacgggcgg aaatttacgc aggagcattg caaggtcagg gcttagaact ggcttatagt
    10381 aattcaatgc tcgataactt cttattaggt gttcaaggta gcggttacgt tgactttggt
    10441 gatggtcgct taaactattt tgcttatgca gggcaaaatg gctttaaata tgccagcgtt
    10501 gggcgtttat tagtagaaga tggcgaaatt ccgaaagaga aaatgtcagt gcaggctatc
    10561 cgtgaatggg ggcaacgtaa ccctagccgt gttcaaggtt tgttggagcg taatccatcg
    10621 tatgttttct ttaaaaacga tcctaccggt caggtaaaag gctctgccgg tgtgccgtta
    10681 gtaccacttg catctgttgc atcagatcgt agcattgtgc catcaggtag cgtgttgttg
    10741 gtcgaaatgc cgttgattga tagcaaagga aattggaccg gcaaacacga aatgcgttta
    10801 atggtggctc ttgatgttgg cggtgcagtg aaaggccatc acttcgattt atatcaaggg
    10861 attggcgata aagcaggcca tatcgcaggt ttaatgaaac attacggccg tgtttgggta
    10921 ttaaattaat acagggtaac tatgttaaaa aaactatctg ccatattctt acttctaccg
    10981 agcgccgtgc ttgcaaataa tttgcaaaat ataaccgctt caactgagcc taaatataaa
    11041 atctcagaaa tagatgtcag aattttgata cgccagctca ataatattga gcaatgtatc
    11101 tatcctgagt tagctaaacc cggttaccag caaatctacg caaattggaa tctggctgaa
    11161 aatttaacga tgcaatattt tgaatatcag cttctaaaag agttgctcgg agaggaaaat
    11221 cagaaactga tgcaaaatga tacgctttct accgagtatt tccatttact gcattctcgt
    11281 ttaaatcacc aaaaagcaaa tgtagatccc gaaaaatgcg atacatttaa acctagatat
    11341 aaagagattt ataaaagtat ggaaaatgca ctcactaaga aaaatcaata gtaaaaaact
    11401 cgcagttttc actgcgagtt tcttttatct ttcgttatga atttctaaat tggtggcttg
    11461 ttttgccgcc tctgcttttg ctttatcgtt tcgagatctt gctatactgt ctaaatagca
    11521 tttatctaca tcacctgtaa tatattctcc tgtaaataca gagtcatcaa agcgatgaat
    11581 agtcggattt tccagttgaa tagacttata aagtgcttca aggtcttgga aaatcaattt
    11641 atccacccct atcatctgtg caacctcttc cactgagcga tcataagcca ctaattcttc
    11701 acaagtcggc atatcaatgc catacacatt cgggtagcga atttccggtg cggcagaggc
    11761 aaaataaacc cgttttgcac cagctgctcg tgccatttcc acgatttgtt cagacgttgt
    11821 acctcgtaca atagaatcat caaccagtaa cacgcttttg cctttaaatt cagaggcaat
    11881 cgcatttaat ttacggcgaa ccgagctttt acgctgtgct tgccccggca taataaaagt
    11941 tcgagctaca tagcggtttt taacaaaccc ttgacgatag ggtttataca acatattagc
    12001 aatacgtacc gcaatatcat ttgaggtttc aggaatcggg atcaccacat caatatcatc
    12061 gataattcgt ccccattcac gtttaatttt ctcacctaat aattcgccca tatgcactcg
    12121 tgcagaatat acagaaacgc catcaatgac ggaatcagga cgggcaaaat aaacatattc
    12181 aaaaatacaa ggattcagtt ttggattatc ggcacaaatt tgcgaatgta attgcccatc
    12241 aaaggtaaca taaatcgctt cacccggcag cacatctcgc acaaattcaa accccactac
    12301 atcaagagcc acactttccg aagcaaacat atattcggtt ttaccctcga tttcacgttt
    12361 acccagcact aacgggcgaa taccgaatgg gtcacggaaa gcaaccatac cgtgtccgat
    12421 aatcatcgca atgcaagcat aagcaccacg aatgctatca ttggttttac gaaccgtttc
    12481 aaagatattg tctgggctta aatgctgagt ggaatagaga tctaaaaagt aagcaaaaat
    12541 attaagaagg gattcagaat cagaattagt gttcacatgg cggcgggctt cgttgtataa
    12601 gcgagcttta agttccgcat tattagttaa attaccgttg tgaactaagg taataccgaa
    12661 aggtgaattg acataaaatg gctgggcttc agacacactt gagctacctg ctgttgggta
    12721 gcgaacgtga ccaattccaa cattgccttg taatctcacc atatgctctt gctggaaaac
    12781 atcgctgact aagccgttag ctttgcgtaa gcggaaacga ttttcatcgt ctatggtgac
    12841 gatacctgcg gcatcttgtc ctcggtgttg aagtaatgtt aaaccatcat aaatcgcctg
    12901 attcaccggc gaattcccaa taataccgac aatgccgcac atatagaaaa acctctttat
    12961 ttagtcgtgt ttaaaaaact ggaatttgct tgtaactgtt caaagaacca cttcacaata
    13021 aagtcaaaat gcggaattaa tagcgatgat ttccataatt ccgtttggct tgcttgggta
    13081 aaggtatcta agaaaaataa aatagccgac acaattaaaa taccacgcaa tgccccaaaa
    13141 gcaccgccta aaatacggtc ggtgatagac attccgctca aatcaatcac tttgcctaat
    13201 aaaccgttga tcacaccaca aacaattaaa gtcaaaatga acagaatcgc cgctgcaata
    13261 ccattgcgta aaaattctga attttgcaaa taaaccgaat taatttgtgt gagataaccg
    13321 cttaaataag gataaaacga gcttgccaca aaaaaggcga ttacccaacc aagtagcgat
    13381 aatgcttcat tggtaaaacc tcgccataaa ctgataagca ctgaaaaagc aatgattccg
    13441 ataacaatta aatcaaccat aacacacctt aaagcgtaaa gctctcctat tctacaaaaa
    13501 aagaaaacgt ttgcgtagca ctatttcgct aaaaataagt gctttatgca aatacaagcg
    13561 gttaaattcg gtaaaaaatt tacacaatcc gaccgcttgt aaaacctagt ctgcaatata
    13621 accaacatcg tccaacgttg gattatttgc tccttgttta gccagctcat cgcaaatctc
    13681 attttcacga tgtcctgaat gccctttcac ccaactccat tcaatttggt ggcgttgaat
    13741 ttcttggtct aaagcaaccc ataaatcttt atttttaact ggctttttat cgctggtttt
    13801 ccagtcattt ttcttccagt taaaaatcca tttttggatc ccgtttttca tatattggct
    13861 gtcactattg agttgcacct tacaaggctc ttttagcatt gctaaagcct caatcaccgc
    13921 ccgcaattcc attcggttat tggtggtttg aaaatagcct ttgctcacgg ttttttcgta
    13981 cccgttataa cgcagcaaaa taccaatccc ccctttgccg gggttgccta aacaagaacc
    14041 gtctgtaaaa atttcaacta atttcatcat cacgctttga aaataaaagc cccaaagcat
    14101 taactttggg gcagctttta ggctaaattc taaaagttat cagaatggag atcaactgat
    14161 tatttttttc caagttgtgg aatcacgcta ttattagccg ccaataaacc tgcatcggta
    14221 taaactgcta atttaccacg ggtatcgaca atatctaagt tacgcattgt taattgaccg
    14281 atacgatctg ccggcgtaaa cggtgcatct tctacttttt ccatacttaa acgttctgct
    14341 tcgtaggtta agtttggcga ttcagtgtta agaatagtga aatcgttacc acggcgtaac
    14401 tcaagcgtga ccgtaccggt aatcgctttt gccacccaac gttgagccgt ttcacgcaac
    14461 attaaagctt gcgggtcaaa ccagcggcct tgatacaata aacgccctaa acgtaatccg
    14521 ttaatgcgat attgctcaat cgtatcttcg ttgtgaatac cggtaactaa acgctcgtaa
    14581 gcgatgtgta ataatgccat tcccggtgct tcataaatac cacgagattt cgcttcgata
    14641 atacggtttt cgatttggtc tgacattccc aaaccatgtc tgccaccaat gcggttagcc
    14701 tccataatta attcaacggc atcatcaata cgtttaccgt ttaacgccac cggcacacct
    14761 tcttcaaagg taacagaaac agtttccgct ttaatttcaa ctgattcgtc ccagaatgcc
    14821 acgcccataa tcggttttac gatcttcata ccggtgctta attcttctaa atctttcgct
    14881 tcgtgggttg cacctaacat attcgagtct gtagagtagg ctttttccac cgacatttta
    14941 tagtcaaaac cgttttcgat taagaattta gacatttcca agcgaccgcc taattcatca
    15001 atgaatagtt ggtctaacca cggcttataa atttttaatt ttggattggt taataaaccg
    15061 taacgataga aacgctcaat atcgttacct ttaaaggttg aaccatcgcc ccagatattc
    15121 acatcatctt ctttcattgc cgcaaccaac attgtgccgg ttaccgcacg accaagtggc
    15181 gtggtgttga aatacggaat accgccggtt gaaatatgga aagcaccgca ttgaattgcg
    15241 gcaatgcctt cgtgagctaa ctgttggcga cagtcgatta atcgtgcatt ttcagcccca
    15301 tattccatcg cttttttcgg aattgcgttg taatcatctt catccggctg acctaaattt
    15361 gcggtgtacg cataaggcac tgcccctttt ttacgcatcc ataacagtgc tgctgaggta
    15421 tctaaaccgc ctgaaaaggc gatacccact ttctgcccta aaggtaaagc ttctaaaatt
    15481 gttgctgaca tacgaattcc tataaatttt gaaagtaaaa atattcaaac gcaaacgctt
    15541 acgttcaagc aaaatattct agctgaaaag tagtaaaaat tcatctaaaa tttagcccga
    15601 tgatttgcaa attttttgtg aaatttgacc gcttataatt tactgaaaac ctaaaagcaa
    15661 tagagaaaat cagaatgaaa aaactactcg taatccgtaa tgataaaatt ggggacttta
    15721 tggtctgctt tccggcattt gctatgttga agcaatcaat gccgaatgtg gaaattaccg
    15781 ctcttgtgcc aagttatact gctccacttg ccgagctttg cccttctatt gataagatta
    15841 ttattgatac ggcgaataaa aaagataaaa atgaatttaa ccgtatttta caagcggtta
    15901 aaaatgagca atttgatgcg gttatttgtt ttgtttcaga ctggcataat gccaaactca
    15961 cctggcaaag tgggattaaa taccgcctcg ctccagcaac taaattattc caatttttat
    16021 ataaccaccg tttaacccaa cgccgttctc aatcagcaaa agcggaatcc gaatataatt
    16081 tggatttagc ccgtgctttt ttaagcaaac acaatatacc gattgttgaa cctcacacgc
    16141 catacttaag tgttgaacag caaaccgtcc aggcacaaaa agcaaaatta tgcgaacaac
    16201 tcaatattac caccgttaaa aagtgggtat ttgtgcatag ttcaaccggt ggttcggcaa
    16261 ataatttatc tatagaacag tatgcagagt taattcaagg tattttggca gaatttgatt
    16321 gctatgtggt actcaccgca gggaaaggcg aaagcgaaaa agctcagcag ttagctcaac
    16381 aaatcaatca caacaacgtg gtggtttatg ataaaaatga ggggttacaa gattttacac
    16441 attcgcttgc gtgtgcagat ttgtttattg ccggctcaac cggtccgtta catattagtg
    16501 gggcgttaaa tattccaact attggcttct acccgagccg actttctgcc attccacgcc
    16561 gttggaaacc gattaatgat gcgaataaac acattgcatt ttgcccaccg tctgataaag
    16621 cctcacaaat gaatttaact ttaatttcta tttctgaggc attaaaaacg atagtaccgt
    16681 ttattcggag aatgtggaat actccctctc tctgataaaa ttgctaaacg caattttatc
    16741 tatctctctc ccactagagg gagagagtac tgaaaagcat catttccgag tacgattcca
    16801 caaatccgca tattttacga aagtagaatg tgccgaaaga actgccaata aaaatccttg
    16861 cttgccatct aaaaaaccgg cttttaatag atacattttc acaaaacagc cgatggcgtg
    16921 agtcacgcca tcaaataagc tggctgtttt gcctgcattt gcccgttgga ttgcccacgc
    16981 tttagcgtaa cttgccgatt ttaccaaata gtggtgaata tctttatagg tgaaatgcag
    17041 caaatcgcct tgtagtttct ggatttttgc ctcttttggg taatgtactt tttcgtgaac
    17101 caattcatca ccatatttgg caaattccgt gcgatacaat ctcaccacac aatccggata
    17161 ccaacccgag tggcgaattt gtttgccaaa tacttcactt aagcggctga ttttgtaaac
    17221 ggtatttttt tcatcatttt tgaccgcttg taaaatagat tgcttcagtt cggctgtcac
    17281 tcgctcatct gcatcaagcc aaagtacata atcactggtc acatattgtt gagctaactg
    17341 acgctgttta ccaaagcctt gccaatcggt gttagtgtag aaccttgcgt tgtattcagc
    17401 agcaatttgt ggtgtttcat cagtactgcc actatctaaa atcacaattt catctaccca
    17461 atcttttact gtgtcaaggc aagcccgtaa atcttgagct tcatttttga caatcatcgc
    17521 aacacttaat gtcggcatag tttatccttc tctctttttg tcgtgtgttt ttctgctatt
    17581 cgttgcattt tactttaaaa atagtggttt ttgtgctatt ttctacgcca aattttatca
    17641 acaaaacaaa ttaacggaaa aagaatgtca attacattaa caaccaaaca aaaacaattc
    17701 ctaaaaggct tggctcatca tttaagccca gtggtgatgc tggggggtaa cggcttaaca
    17761 gaaggcgtgt tagctgaaat cgataacgcc ttagaccacc acgaactgat taaagttaaa
    17821 gttgccgggg ctgatcgtga aaccaaacaa ttaattattg atgccattct gcgtgaaact
    17881 aaagcggctg aagtacaaac cattgggcac attttagtgc tttaccgcca aagcgaagag
    17941 aaaaaaatca gcttgccaaa agcaactaaa gcgatctaat tgcaaaaata tgtttaaatt
    18001 caaccgcttg tattcaagcg gttttttata ggactttcta tgtttaaaaa attctctctt
    18061 tatacccttg caatgttgct cattcctgtg gtgacttggc tggcaaattg gcactggtct
    18121 ctggcaattc aatacgctga atttgatttc gactactttt tatatttaat caccgaaacc
    18181 ggctccgtac cttatgccat catcacctgt gtggttttcg ccttattact ggcgagggtg
    18241 gttcgcaaac gctactcttt aatttttgtg attaccgtgt gtgtcgcttc aatggtagcc
    18301 actcaagcaa tcaaaacagc gatgaaatca acctttcaag agcctcgtcc atttgtcact
    18361 gctcttttcc aagataaaac agacgatttt tatcaattaa cacgcgagca acgtgggctt
    18421 gccgtaatgg aaaaaatcgg tgcaaatcaa aattttgtta ccgctcacca agccaaagaa
    18481 gtgggctatt ctttcccatc aggccacact atttttgcgg tttcttggct attattggtg
    18541 gtaggtttct gccgtaatat gcgaggacaa gcggtcgttt tggctcattt ttttgcaatc
    18601 agttgggcgg cgttaatgct gattagccgc ttgcgtttag gtatgcacta cccgattgat
    18661 ttattcacca gtagtttaat tgcgtttcta atcaatttag ccttatttgt gtggtttgtg
    18721 ccttggttgg aaagtaaaaa atggtttgct aaaaagcc
//
LOCUS       DANLXS010000029        18121 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.029, whole genome shotgun sequence.
ACCESSION   DANLXS010000029 DANLXS010000000
VERSION     DANLXS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18121
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.029"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(81..4067)
                     /gene="hrpA"
                     /locus_tag="PXD71_000780"
     CDS             complement(81..4067)
                     /gene="hrpA"
                     /locus_tag="PXD71_000780"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDL6290930.1"
                     /translation="METPLQKNHKKPPLVKLENITTADFRRLLGRLKGAENIKNIDHK
                     RSVLAEISQDLTACQARFFARTQHAQNLKIDYPDLPVSARREEILKLISENQVVVIAG
                     ETGSGKTTQLPKMCLELGRGVKGLIGHTQPRRIAARSVATRIAEELKSELGETVGYKV
                     RFNDQVSENSLIKLMTDGILLAEIQHDSYLNQYDTLIIDEAHERSLNNDFILGYLKQI
                     LHKRPDLKVIITSATIDVERFSKHFNNAPIIEVSGRTFPVEVRYRPIVEDQDQLQGIL
                     NAVDELQAEGRGDILIFMNGEREIRDTAEALQKQELRHSEILPLYARLSAAEQQRIFQ
                     PSGGLNRIILATNVAETSLTIPNIKYVIDTGTARISRYSYRTKVQRLPIEPISQASAN
                     QRKGRCGRTSEGICIRLYSEEDFNSRPQFTDPEILRTNLAAVILQMTSLGLSDIANFP
                     FVDAPDSRQIQDGIRLLEELQAIIPNEKIPSTACGGGLGRGEIRSNDGTPPLPTSPRK
                     RGDGFRLTPIGRQLAQLPVDPRLGRMVIEAAKNGSLHEVMMIVSALSIQDPRERPQEK
                     QQSADDKHRRFADKESDFLAFVNLWHFIQAQQKELSKNQFRNLCRKDYLNYNRVREWQ
                     DIYHQLRLAVREMGLKINSEPASYQPIHTALLAGLLSHIGLKDNEKMHYLGARNAHFF
                     IFPNSALFKKQPKWVMAAELVETTKLWGRMVARIEPEWVEPLAVHLTKSSYSEPHWAK
                     SKGAVMASEKVSLYGIPIVAQRSVNYGSIDPTVSREIFIRSALVEGECHNNYKFFKEN
                     HRLIREVEALEHKSRRRDILVDEQVLFEFYDQRIGTEVVSSKHFDTWWKQASKKDPEL
                     LNFEKSFLINDNANLASELDFPNFWHQGDLKLKLSYQFEVGKDHDGVTVHIPLPLLNQ
                     VEPEGFDWQIPGLRHELIVSLIKSLPKSLRRNFVPAPNYADAFLGRAEPFKKPLLDNL
                     SHELRRMTGVLVEPEQWDLSQLPPHLRMTFRVIDDKGKKLQESENLDELKFALKDEVQ
                     NTLSNIADDGIEQSGVHIWNFAELPQFYEQKKAHFSVKAYPAIVDEQTAVGVKLFETE
                     FEQARAMQAGLRRLLLLNVPSPIKYLHEKLPNKAKLGLYFAPFGKVLELIDDCIACAV
                     DQLVEEFGGFVWTEEKFTQLHEFVRANLNDKTVEIAKQVEKCLTLAYELNKRLKGKLD
                     FTMAFALSDIKTQIEGLIYPDFVTKTGYARLADLYRYLTAIERRLDKLLVDSNTDRAK
                     MLRVEAVQEAYKQLLAKLPKSKAVPDEVLEIRYMIEELRVSLFAQQLGTKYPISDKRI
                     LNVINEIK"
     gene            complement(4171..4623)
                     /locus_tag="PXD71_000781"
     CDS             complement(4171..4623)
                     /locus_tag="PXD71_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL6290931.1"
                     /translation="MTLQFNAVALLLVVLIILGLISQNSAVTISAAVLLIMQQTLLSK
                     FIPFVDQYGLKIGIIILTIGVLSPLVSGRITLPELAQLLNWKMALSIIAGVLVAWLGG
                     RGVNLMGSQPVLVTGLLIGTVIGVAFLKGVPVGPLIAAGILSLIVGKT"
     gene            complement(4686..4955)
                     /locus_tag="PXD71_000782"
     CDS             complement(4686..4955)
                     /locus_tag="PXD71_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290932.1"
                     /translation="MDAAMSLRFDKLRFVKRLQEANQTPEMAEAFANALDEALEQSQS
                     PLATKADLQLELEKLKNEINTTIFKAITLNITILGFLMAIMKFIN"
     gene            complement(5053..5649)
                     /gene="grpE"
                     /locus_tag="PXD71_000783"
     CDS             complement(5053..5649)
                     /gene="grpE"
                     /locus_tag="PXD71_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL6290933.1"
                     /translation="MTTQNEHLQEEVKQEEIQPEVVTEETQAEEQNTPQEEPLALAQA
                     RIAELEAYIAEADKREQDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVD
                     NLERGLDALDKAVTDETTQALVDGVEMTHKEFINTLAKFGVVAIGEVGEAFNPELHEA
                     ISMQPAEGIEANHVSTVLQKGYTLHGRVIRPAMVMVAN"
     gene            5871..6965
                     /locus_tag="PXD71_000784"
     CDS             5871..6965
                     /locus_tag="PXD71_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL6290934.1"
                     /translation="MKKLAGLFAAGLATVALTACNEEKAAEPKATAAEAKPQATNQTV
                     HLYTWTEYVPEGLLDEFTKETGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYF
                     VSKMAKEGMLAELDHAQLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNTADYK
                     GETFTSWGDLWKPEFAGKVQLLDDAREVFNIALLKLGKNPNTTNPDEIKEAFEELKKL
                     RPNVLSFTSDNPANSFIAGEVSVGQLWNGSVRIAKKENAPVDMVFPKEGPVLWVDTLA
                     IPANAKNKENAHKLINYLLSAKVAEKLTLAIGYPTSNVEALKVLPKEITEDTAIYPSA
                     EVLQKSQWQDDVGDAIELYEKYYQELKAAK"
     gene            7101..8492
                     /locus_tag="PXD71_000785"
     CDS             7101..8492
                     /locus_tag="PXD71_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL6290935.1"
                     /translation="MNLRWQEYPENARKLFKLSLPIFISQLSASGMGLADIVMAGLVS
                     DDDVSAIAVSNSIYFPLFLFVSGLLNAITPTVSYLNGSSNRHLIANQVRQGYWIVFAM
                     SIPLMAIFLNSHLILDLMQTPAEFSIKSQQYLAVMAVGLIPALLAVSLRCMNDGLSNP
                     KPAMRITFFGLLLNIPLNYIFIFGKFGLPEMGAVGCGVATAIVNLIMFFMLLHYCYTN
                     SAQKDIRLFEKWIDAPSGQILLKLCKLGLPIAFAIFTEVMLFSTSSLFLSPLGSQVVA
                     SHQAALQTSSLFFMIPMSFGIATTIMIGQTLGQKNVEGAKILSYHALITATALAIIAA
                     IIIILLKNFIPFAFTNNPISIAITAHLLIFAAVYQIPDAIQAVANGILRGYKHTQPIL
                     YVTLFCYWGVSMPLGYILARTDWIVQPMAAQGFWLVFCIGLSLAGGLLLWQMRKIQAV
                     PKAQLIAKLERIK"
     gene            8551..12075
                     /gene="recB"
                     /locus_tag="PXD71_000786"
     CDS             8551..12075
                     /gene="recB"
                     /locus_tag="PXD71_000786"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDL6290936.1"
                     /translation="MKNLNPIQLPLNKSVLIEASAGTGKTFTIANLYLRLLLGIGCNP
                     LTVEQILVVTFTKAATEELKNRIRKNIQQCADFLKDQADGLEVESTKSYRNNLDFLAQ
                     IYPLIPNIHEALLRLSIAEREIDTASVFTIHGFCQKMLVQFAFESGVRFDLDLQPNQS
                     DLLKKLSEEVWREQFYPQDLAITYAVAEQLGTPEYALNAVRRYLSTELPEPNASLNQD
                     IAEHLAQYQQFINEVKSYWKTHSDELVETIRHDLKHKITLHGGWYRATTFEAGVEVMN
                     QWANSQHNHFPAGLYLFTTEKIAAGTKKSCEPIDSPHLAKNQAFLTAYKERFENSLSA
                     MLQYQFLIALKAKLAEYKQSHPQRSFDDLLTMLNQALKNLHTGEALSQKIRAMYPFAM
                     IDEFQDTDLTQYEIFSQIFMAENAENGFIMIGDPKQSIYKFRGADIFTYLRAAKQADE
                     QFTLGKNWRSLPEVVQAVNNLFSFANDNSPFLYEDIGFHPVEADSQKGQLVSEQGHFV
                     GYITENSKLDDLAEMCAYQIHQQLEAMAQGKFGVEKNGEFQSFAAKDIAILVRKGSQA
                     KRIKKALAKRNIKSVYLAESASVFGSEIATELCWLLQAALNPYHYKALLSALGSTLWG
                     LTAAEIHRYKENEQLWDLKVNQFVEYSNIWKEQGVLPMLHKIYLEQGIIERLKGLENA
                     DRLITDLLHLTEILQETSQALESEAELLRWYEKQVNSPDFNDEQQLRLESEEELIKIV
                     TIHGSKGLQYPIVWLPFVSQKSEGDTQTALSLYRNSQGALDWCLGSEEASFSLQYEAY
                     AEDLRLLYVALTRAESQLNLVLPKEISGWSPLHYLLADGEIPTGKVPVSDYFSQKRIA
                     ANLVEVAGELPFSLWEGEQSSDDYQAKTFSGNIRVVGQITSFTSLYAQHERFSEGKSE
                     RVMDLAQDRDVHIETIEAGYSSETAAFSPFQFPHGIKVGTVLHHFFEHCQFNEQIDRE
                     AVAKVCEQLGLSEEWIEPTALWFERILTTPLAEANFCLKAIDETKRLNEWQFYLRLKN
                     DKALHQLNALLKQHSPLAKTLPELQLPQLEGFVRGFVDCIVQVEEKFYLIDYKSNFLG
                     YLPQNYAKEHIQREMGRQRYDLQYLLYTLALHRYLTARLGEKYDYERDFGGVAYLFLR
                     AMNGENDNGVYFDKPTKQLIEEMDRLFG"
     gene            complement(12117..12599)
                     /gene="greB"
                     /locus_tag="PXD71_000787"
     CDS             complement(12117..12599)
                     /gene="greB"
                     /locus_tag="PXD71_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDL6290937.1"
                     /translation="MAKSNYITRSGWQALDQELKFLWREERPKVTQAVSDAAALGDRS
                     ENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEEGEAK
                     QYRIVGCDEFDPAKNWISIDSPVARALIGKQVDDEIRVETPLGKVLLYINKIWYEQAS
                     "
     gene            complement(12650..13930)
                     /gene="pepB"
                     /locus_tag="PXD71_000788"
     CDS             complement(12650..13930)
                     /gene="pepB"
                     /locus_tag="PXD71_000788"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDL6290938.1"
                     /translation="MTAMKISLSTQNAPEIWGKNAILSANDAGFTVHNPNNDLATIQK
                     AARKIKNQGISTVELTGDSWDLESCWAFHQGFHSVKNQGSVQYPDLDEQQAEFDNRVK
                     CATFVRELINKPSDKLTPEKLAEQAVEFIQKMAKPNTVSYQIIRGEALQEQGYHGIWT
                     VGKGSANPPAFLVLDFNPTNDENAQVLACLVGKGITFDTGGYSLKPSDSMSTMRTDMG
                     GAALLTGSLAFAIARGLNKRVKLYLCCAENMVSHNAFKLGDIITYRNGVTVEVLNTDA
                     EGRLVLADGLIDACKQNPQFIIDAATLTGAAKVAVGNDYHSVLSMDDNLTEALFKSAK
                     TENEPFWRLPFEEFHRGQIASSFADIANIGSVPVGAGASTATAFLSYFVENYQQNWLH
                     IDASATFRKTASDLWAAGATGLGIRTVANLLLSK"
     gene            complement(14069..14890)
                     /locus_tag="PXD71_000789"
     CDS             complement(14069..14890)
                     /locus_tag="PXD71_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDL6290939.1"
                     /translation="MKNIKLSAMSLTALFVLTACSHTHKNSDEHHQMVLQEQATLGIN
                     WMQQSGEYQALAHQAFNAAKVAFDQAKVKKGKKKAVVVDLDETMVDNSLYAGWQVKNH
                     KPFDGESWTRWVNARQTGAIAGAVEFNNYVNSHKGTMFYVSNRKDSSEKAGTIDDMNK
                     LGFSGVSEQTLLLKKDKSNKTPRFEEIEKQGYEIVLYIGDNLNDFGDATYRKSNAERR
                     AFVAENSQQFGKKFIMLPNPNYGDWEGGLDKDYYKGNAESRVKIRHNAIKAWDGK"
     gene            15038..15981
                     /locus_tag="PXD71_000790"
                     /pseudo
     CDS             15038..15981
                     /locus_tag="PXD71_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(16094..17257)
                     /locus_tag="PXD71_000791"
     CDS             complement(16094..17257)
                     /locus_tag="PXD71_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL6290940.1"
                     /translation="MKRKTLATLVLIAALGIGGYFYYQQQTNKPQIHYITQAVTRAEI
                     NKNVLATGSVRANKRTEVGAQVSGKIQKLYVELGQSVKQGDLIADIDSSNQSNTLSTA
                     QARLSSYKAQLTSAQVALEVAQSNYNRLNKLYRANSASLNDLETAKNTLASAKATVDN
                     IKAQLKSAEISVNDAKTNLTYTQIISPMDGVIVSVPVSVGQTVNSNQTSPTIVQVADL
                     SKMLIKLEISEGDIAQVKVGQKVKFSTLAEPNREYSATIDSVDPALTTLTDNNYTEQS
                     GNTEAVYYYANVSVDNADNSLRIGMTTQAQITIDKREQVLVVPTSVIKKRGKENYVLV
                     LENEQSVERKIQIGISDTQNTEVLAGLNEGDQVIVTQRADDEKISTARGPRMF"
     gene            complement(17323..>18121)
                     /locus_tag="PXD71_000792"
     CDS             complement(17323..>18121)
                     /locus_tag="PXD71_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL6290941.1"
                     /translation="AIISDLDGTLLNANHKIGDFTIDTLEQLSKKGVDIFFATGRNHP
                     DVSQIIGKVNVKNAMLVTSNGARANNLEGKKILNHYLPEDLAFELMNIEFDDQNVCLN
                     SYQGDEWFINKEIAELKKFHQDSGFSYQVVNFAHHHGRNTEKVFFIGKTAEALVPIEH
                     YIRDTYGDRVQMTYSALLCLEVMAKGVCKANALAELVKLRGYTLADCIAFGDGMNDVE
                     MLSLAGKGCLMGNADPRLKAVLPNNEVIGFNKDEAVASYLRATFGIV"
BASE COUNT         4897 a         3795 c         3940 g         5489 t
ORIGIN      
        1 gaggaatagg tattcgagaa aacaagcggt cagagttgca aaatttttta cttatctgac
       61 cgcttgttgt atagggaata ttactttatt tcattgatca cattcaaaat ccgcttatcc
      121 gaaatcgggt attttgtgcc gagctgttga gcgaacagac tgacccgcag ttcttcgatc
      181 atatatcgaa tttccagtac ctcgtccggc accgctttgg attttggcag tttggcaagg
      241 agctgtttat atgcctcttg cactgcttcc acccgtagca ttttggctcg gtcggtgttg
      301 ctgtccacca acagtttatc caaacggcgt tcgatagcgg tcaaataacg gtataaatct
      361 gcaaggcggg cgtagccggt tttggtgaca aagtcggggt aaatcagccc ttcgatttgg
      421 gttttgatgt cgcttaacgc aaacgccatc gtaaaatcga gcttgccttt taaccgcttg
      481 tttagctcgt aggccagtgt gaggcacttt tcaacctgtt tggcaatttc tacggtttta
      541 tcgtttaaat ttgcccgcac aaattcgtgc agttgggtga atttttcctc cgtccagaca
      601 aacccgccga actcttctac cagctgatct accgcacagg caatgcagtc gtcaatcagc
      661 tccaatactt tgccgaacgg ggcaaaatat aagcccagct tggctttgtt cggcagtttt
      721 tcgtgtagat atttaatcgg cgatggcaca tttagcaaca gcagacggcg aagtcctgcc
      781 tgcattgcac gagcctgctc aaattccgtt tcaaacagtt tcacccccac agccgtttgt
      841 tcatcgacaa tcgcagggta ggctttcacg ctgaaatggg cttttttctg ctcatagaac
      901 tgtggcaatt cggcaaaatt ccaaatatgc acaccacttt gctcaatgcc gtcatcggca
      961 atgttggaaa gggtgttctg cacctcatct ttcaaggcaa atttcagctc atcaaggttt
     1021 tcgctctctt gcagtttctt gcctttatca tcaatcacac ggaaagtcat ccgcaaatgt
     1081 ggcggcagtt ggcttaaatc ccactgttca ggctcgacca atacacccgt cattcggcgt
     1141 agttcgtggc ttagattgtc cagcaacggt tttttaaacg gctcggctcg ccctaaaaag
     1201 gcgtccgcat aattcggcgc cggcacaaaa tttcgccgta gcgatttcgg taaagattta
     1261 atcagcgaga cgatcagctc gtggcgtagc cccggaattt gccaatcgaa accttcgggt
     1321 tccacttggt taagcagcgg tagcggaata tgcaccgtca cgccatcgtg atcttttccg
     1381 acctcaaact gatagctcag tttgagtttt aaatcgcctt gatgccagaa attcgggaaa
     1441 tccagctcgc tggcaaggtt ggcgttgtcg ttgatcagga acgatttttc aaagttcagc
     1501 agttcaggat cttttttgct tgcctgtttc caccaagtat caaaatgctt gctcgacaca
     1561 acttcggtgc caatccgctg atcgtaaaac tcaaacagca cctgctcgtc caccaaaata
     1621 tcacggcggc gggatttgtg ttccaatgcc tcaacttcac ggatcaagcg gtgattttct
     1681 ttgaaaaatt tgtaattgtt gtgacattcg ccttccacca acgccgagcg gataaaaatc
     1741 tcacggctga cggttgggtc tatcgagcca taattcaccg agcgttgtgc cacaatcgga
     1801 atgccgtaga gcgacacttt ttccgaggcc attaccgctc cttttgactt cgcccagtgt
     1861 ggttcgctgt aactggattt agtgaggtgt actgccaacg gttccaccca ttccggctca
     1921 atgcgagcga ccattcgtcc ccaaagtttg gtagtttcca ccagctctgc ggccattacc
     1981 catttcggct gttttttgaa aagtgccgag ttcgggaaaa tgaaaaagtg ggcatttctt
     2041 gctcctaaat agtgcatttt ttcattatct ttcaagccga tatgcgacaa caagccggcg
     2101 agcagggcgg tgtgaatcgg ctgataactt gccggctcac tgttaatttt taagcccatt
     2161 tctctaactg caaggcggag ttggtggtag atatcctgcc actcccgcac acggttgtag
     2221 ttcaaataat ccttacggca gagattacgg aactggtttt tcgacagttc tttttgttgg
     2281 gcttggataa agtgccacaa attcacaaag gctaagaaat cgctctcttt atcggcaaaa
     2341 cggcggtgtt tatcgtctgc cgactgctgt ttttcctgcg gacgctcacg agggtcttgg
     2401 attgagagag ctgacacaat catcatcact tcgtgcaacg aaccgttttt cgccgcctcg
     2461 atcaccattc tgcctaagcg aggatcgact ggcagttggg cgagttggcg accgattggg
     2521 gttaatctaa acccatcccc ccgtttacgg ggggaggttg ggaggggggg agtgccatca
     2581 ttagaacgga tttcccccct ccctagccct cccccgcaag cggtggaggg gattttttca
     2641 tttggaataa tcgcctgtaa ttcttccaac aaccgaatcc cgtcctgaat ttgtcggctg
     2701 tcaggggcat caacaaacgg gaaattggca atatcactca atccaagcga agtcatttgt
     2761 aaaataaccg ctgcaaggtt agttcgtaga atttcaggat cggtaaattg tggacggctg
     2821 ttgaaatcct cttctgaata gaggcggata cagatccctt ccgaggttcg cccacaacgc
     2881 cctttccgct ggttggcgga ggcttgcgaa atcggctcaa tcggcaagcg ttgcactttg
     2941 gtgcggtagc tataacgaga aatacgagcc gtgccggtgt cgatcacata tttaatattc
     3001 ggaatagtga gggaggtttc tgccacgttg gtcgccagaa taattcggtt tagcccaccg
     3061 cttggctgga aaatccgttg ttgttcggca gccgataagc gggcgtaaag gggtaaaatt
     3121 tcgctatgtc gcagttcctg cttttgcaag gcttcggcag tgtcacgaat ttcccgttca
     3181 ccgttcataa aaatcagaat atcgccacgc ccttcggctt gcagttcgtc caccgcattt
     3241 agaatacctt gcagttgatc ttgatcttcc actatcgggc gataacgcac ttccaccggg
     3301 aatgtccgac cagagacttc aataatcggg gcgttgttga aatgtttgga aaaacgttct
     3361 acatcaatag ttgccgaggt gatgatcact ttcagatcgg ggcgtttgtg cagaatttgt
     3421 ttcagataac ccaaaataaa atcgttgttc agcgaacgct cgtgagcttc gtcaataatc
     3481 agcgtatcgt actgatttaa atagctgtcg tgctggattt ccgccagcag aataccgtcc
     3541 gtcattagct tgattaagct gttttcactg acttgatcgt taaagcggac tttgtaaccg
     3601 accgtctcgc ccagttccga tttcagctct tcggcaatgc gagtcgccac cgaacgtgcg
     3661 gcaattcggc ggggctgggt atgcccaatc aagcccttca ccccacgccc gagttctaag
     3721 cacattttcg gtaactgggt ggttttgccc gaaccggttt cccctgcgat caccaccacc
     3781 tgattttcgc taatcagttt taaaatttcc tcacggcgag cggaaacagg cagatcaggg
     3841 tagtcaattt tcaggttttg ggcgtgctga gttcgtgcga aaaaacgtgc ctgacaagcg
     3901 gtgagatctt gcgaaatttc tgccaaaact gaccgcttgt gatcgatgtt tttaatattt
     3961 tccgcccctt ttaagcgacc gagcaggcgg cggaaatcgg cggtggtgat attttccagc
     4021 ttcacaagcg gtggtttctt atgatttttt tgcaaaggtg tttccattta atttctcttt
     4081 tgcattaaga ccttgtgatt tattgtaggg tgggcgtaag cccacgtaac agaataggga
     4141 ttttatgcgt ggacttacgt ccaccctaca ttaggtttta ccaacaatca gtgacaaaat
     4201 ccctgccgca atcaacggcc cgaccggtac gccttttaag aacgccacgc cgatcaccgt
     4261 gccgattaaa agcccagtta ccaacaccgg ctggctgccc attaaattca cgcccctgcc
     4321 acctagccac gctaccagca cgccagcaat tattgagagt gccattttcc agttcaatag
     4381 ttgggcaagt tcgggaagcg taattcgccc tgaaaccaag gggctaagta cgccgatggt
     4441 taaaatgata atcccgattt ttaagccgta ttgatcgaca aaggggataa atttagaaag
     4501 taaggtttgt tgcataatca gcaatacggc ggctgaaata gtaaccgcac tgttttggct
     4561 gatcagccct aaaataatca gcaccacaag tagaagtgcc actgcgttaa attgtaaagt
     4621 cataggatta aaacgtaaaa acccttactt catagaagta agggagtggt taattaaacg
     4681 ttgagctagt taataaattt cattattgcc attaaaaagc caagaattgt gatatttaag
     4741 gtaattgctt taaatatcgt ggtattgatt tcatttttta gcttttcaag ttcaagttgc
     4801 aaatcagctt ttgttgctag tgggctttga ctttgctcta aggcttcatc gagagcatta
     4861 gcaaatgcct cagccatttc aggtgtttga ttggcttctt gcaagcgttt tacaaaacgt
     4921 aatttgtcaa atcgtaggct catagctgca tccatttcaa ttatcctctt gtggttaagt
     4981 tataaactaa tttagcataa tcacaagcag gaattttagg agattttgtg atcttcgtcg
     5041 aaaatcgaca atttagtttg ccaccatcac cattgccggg cgaatcactc gtccgtgaag
     5101 cgtgtagcct ttttgtagca ctgtgctgac atggttggct tcaattcctt cggcaggttg
     5161 cattgaaata gcttcgtgca attcagggtt aaatgcctcg cctacttcgc caattgccac
     5221 cacaccaaat ttagccagtg tattgatgaa ttctttatga gtcatttcaa cgccgtcaac
     5281 taacgcttga gtggtttcat cggtcacggc tttatcaagg gcatctaatc cacgttcaag
     5341 gttatcaacc actgttaaca attctttgga gaatttttct aaagcgaatt tgtgagcttt
     5401 ttcaacatct tgctctgcac ggcggcggat attttgaatt tctgcttgag ccctaagctg
     5461 aatatcttgc tcacgtttgt cggcttctgc aatataggct tctaactcgg caattcgagc
     5521 ttgagcgaga gctaacggtt cttcttgagg tgtattttgt tcttccgctt gagtttcttc
     5581 agttacaact tcaggttgaa tttcttcttg ttttacttct tcttgtaagt gttcattttg
     5641 agtggtcatt ttttatcctt taaaagacta atctggaaat gtaaatcttc cttaatatag
     5701 cgataaatta agggatttca agcatatctc tttctttttg cgaaaataat tcacttttta
     5761 atcagaaaag gcttgcaaaa tacggggggg ggcttaaaat gccctagtct tttccgccct
     5821 taggcaaggc tatatttttt aatcactttt ctttaacgga gaaaacttaa atgaaaaaat
     5881 tagcgggttt atttgcagca ggtttagcaa cagttgcatt aacagcgtgt aatgaagaaa
     5941 aagcggcaga gccaaaagca actgcggctg aagcaaaacc tcaagcaaca aatcaaacag
     6001 ttcaccttta tacttggact gaatatgtgc ctgaaggttt gttagatgaa tttacgaaag
     6061 aaaccggcat taaagttgaa gtttcaagtc ttgaatctaa cgaaacaatg tacgctaaat
     6121 taaaattaca aggcaaagat ggcggttatg atgtaattgc tccatcaaac tactttgtat
     6181 ctaaaatggc gaaagaagga atgttagcgg aattagatca cgcacaactt ccggttatta
     6241 aagaattaaa ccaagattgg ttaaataaac cttacgatca aggaaacaaa tattcattac
     6301 ctcaattatt aggtgcgccg ggtattgcat ttaatacggc agattacaaa ggtgaaacat
     6361 tcacttcttg gggtgatttg tggaaacctg aatttgcagg taaagtacaa ttattagatg
     6421 atgctcgtga agtattcaac attgcgttat taaaattagg taaaaaccca aatacaacta
     6481 atccggatga aatcaaagaa gcctttgaag agttgaaaaa attacgtcca aatgtgcttt
     6541 catttacctc tgataacccg gcaaactcat ttattgctgg tgaggtttct gtaggtcagt
     6601 tatggaacgg ttctgtacgt attgcgaaga aagaaaacgc accggttgat atggtattcc
     6661 cgaaagaagg tcctgtgctt tgggtcgata ctttagccat tccggcaaat gcgaaaaaca
     6721 aagaaaatgc acataaatta atcaactact tattaagtgc aaaagtagct gaaaaattaa
     6781 cgttagcgat tggttaccca acttcaaacg tagaagcatt aaaagtatta ccgaaagaaa
     6841 ttactgaaga tacagcaatt tacccatctg cagaggtttt acaaaaatct caatggcaag
     6901 atgatgttgg tgatgcaatt gagctttacg aaaaatacta ccaagagtta aaagcagcaa
     6961 aataatagct gattaattct gaagcctcta gggagcaatc tttagaggct ttctcttttg
     7021 agttgcaaaa aatttggcaa aaacgaccgc tttctaaggt acaatcataa aaacatactt
     7081 agattttgag agattaaacc atgaacttaa gatggcaaga atatcctgaa aatgcccgca
     7141 aattatttaa actatcccta cccattttta tttctcagct ttcagcttcc ggaatggggc
     7201 tggcggatat tgtaatggct ggcttggtga gtgatgatga tgtctcggcg attgcggtaa
     7261 gtaattcgat ttactttccg ctctttctgt ttgtgtcagg gttgctgaat gcaattaccc
     7321 caaccgtttc ttacttaaac ggctcgagca atcgccattt aattgctaac caagtaaggc
     7381 agggctattg gattgtgttt gcaatgtcta ttccattaat ggcaattttc ctaaacagcc
     7441 atctgatttt agacttaatg caaacaccgg ctgagttttc aatcaaatcg caacaatatt
     7501 tagcagtaat ggcagttggg cttattcctg ctttgctggc ggtgagttta cgttgtatga
     7561 acgatggatt atctaaccct aaacctgcga tgcggattac ctttttcggt ttattgctga
     7621 acattccgct aaactatatt ttcattttcg gcaaatttgg cttgcctgaa atgggagcgg
     7681 taggctgtgg tgtagcaacc gcaattgtga acttgattat gttttttatg ttgttgcatt
     7741 attgctacac caattctgcc caaaaagata ttcgtttgtt tgagaaatgg attgatgctc
     7801 caagcggtca aattttgcta aaactttgca aattagggct gccgattgcc tttgcgattt
     7861 ttaccgaagt gatgctgttt tcgacctcat cgctgtttct gtcaccactt ggttcacagg
     7921 tggtggctag ccaccaagct gcattgcaaa ccagctcgct attttttatg attccgatgt
     7981 cgtttggcat tgcaaccacc attatgatcg gacaaacact tgggcagaaa aatgtggagg
     8041 gggcgaaaat cttaagttac cacgcactga ttaccgcaac cgctttagct attattgccg
     8101 ccatcattat tattttgttg aaaaatttta tcccttttgc gtttaccaac aacccgattt
     8161 cgattgcgat taccgctcat ttattgattt ttgcggcggt ttatcaaata ccggatgcca
     8221 ttcaagcggt agcaaacggc attctgcgtg gttataaaca tacccaaccg attttatacg
     8281 ttactctgtt ttgttattgg ggggtgagta tgccgctggg ctacattttg gcaagaaccg
     8341 attggattgt acaaccaatg gctgctcaag gtttttggtt agtcttttgt atcggcttaa
     8401 gtttggctgg tggcttgtta ttatggcaaa tgcgaaaaat acaagcggtg ccaaaagcac
     8461 aattaattgc aaaattagaa cggattaaat aacagattct ctgctatgga acatagcagt
     8521 gctttttact tttagaagat aactttgctg atgaaaaacc taaatcctat ccaactacct
     8581 ttgaataaat ccgttttaat cgaagcgtcg gcaggtacag ggaaaacctt taccattgcc
     8641 aacctgtatt tgcgtttatt gctcggcatt ggttgcaacc cgttaacggt ggaacaaatt
     8701 ttggtggtga catttaccaa agcagcaacc gaagagttga aaaaccgtat ccgtaaaaat
     8761 attcagcaat gtgccgactt tttaaaagat caagctgatg gtttggaagt ggaaagtaca
     8821 aaatcctatc gaaacaacct tgattttttg gcacagattt atccgctcat tccgaatatt
     8881 cacgaagcat tgttacgttt aagtattgcc gaacgggaaa ttgataccgc cagcgtgttt
     8941 accattcacg gcttttgtca aaaaatgctg gttcagtttg cgtttgagtc gggcgtgcgg
     9001 tttgacttgg atttacagcc aaaccaaagt gatctactta aaaaattaag tgaagaagtc
     9061 tggcgagagc aattctatcc gcaagattta gccatcactt atgcggttgc cgaacaactt
     9121 ggtacgccgg aatatgccct caatgcggta aggcgttatt tatctactga attacctgaa
     9181 ccaaatgcct ctttaaatca agatattgcc gagcatttgg ctcaatatca gcagtttatt
     9241 aatgaggtga aatcctattg gaaaacgcat tcagatgaac tggttgaaac tatccgccac
     9301 gatttaaagc ataaaattac gctgcacgga ggttggtatc gagcgaccac ttttgaagcc
     9361 ggtgtggaag tgatgaacca gtgggctaat tcgcaacata accatttccc ggcagggctt
     9421 tatttattca ctacagagaa aattgcggct ggcacgaaaa agagctgtga gccgatagat
     9481 tcccctcatc ttgcaaaaaa tcaggctttt ttgaccgctt acaaggaacg ttttgaaaac
     9541 agcttgtcag caatgttgca gtatcaattt ttaattgctc tcaaagcaaa attggcagag
     9601 tacaaacaat cgcacccaca acgcagtttt gatgatttac tcacaatgct aaaccaagcg
     9661 ttgaaaaatc tacacacggg cgaagccttg tctcagaaaa ttcgggcgat gtatcctttt
     9721 gcgatgatcg atgagttcca agataccgat ttaacccaat atgaaatttt cagccaaatt
     9781 tttatggcag agaatgcaga gaacggcttt attatgattg gcgacccgaa acagtcgatc
     9841 tataaattcc gtggggcgga tattttcacg tatttgcgag cagcaaagca ggctgatgaa
     9901 caattcacat tgggcaaaaa ctggcgttcg ttgcctgaag tggtgcaagc ggtcaataat
     9961 ttgttttctt ttgcaaatga taactctccg tttctttatg aggatattgg ctttcaccca
    10021 gtggaggctg atagccaaaa aggtcagtta gttagtgagc aagggcattt tgtaggctac
    10081 atcacggaaa acagtaaatt agatgatttg gctgaaatgt gtgcttatca aattcatcaa
    10141 cagcttgagg cgatggctca aggcaaattc ggagttgaaa aaaacggcga atttcaatcc
    10201 tttgctgcaa aagatattgc gattttagtc cgaaaaggtt cacaagctaa gcgaatcaaa
    10261 aaggcgttgg caaaacgtaa tatcaaatcc gtctatttag ctgaatcagc ttctgtattt
    10321 ggttcggaaa ttgcaacaga attgtgttgg ttattgcaag ccgcactgaa tccgtatcat
    10381 tacaaggcat tattatcggc gttaggctca accttgtggg gcttaacggc ggcagagatc
    10441 caccgctata aggaaaatga gcaactttgg gatctgaaag tcaatcagtt tgtggaatac
    10501 agcaacattt ggaaagagca gggcgttctg ccgatgttgc ataaaatcta cttggagcag
    10561 ggcattatcg aacgtttgaa aggtttggaa aatgcggatc gcttgattac ggatttattg
    10621 catttaaccg aaattttgca ggaaacctcg caagcgttag agtctgaagc tgaactgttg
    10681 cggtggtatg aaaagcaagt taattcgccc gattttaatg acgagcaaca acttcggttg
    10741 gaaagtgaag aggagctaat caaaattgtg acgattcacg gctcgaaagg cttgcaatat
    10801 ccaattgttt ggttaccttt tgtttcacaa aaaagtgagg gagacacgca gacagcatta
    10861 agtttatatc gcaattctca aggtgcgctg gattggtgtt taggctcaga agaggcaagt
    10921 ttttcgctac agtatgaggc ttatgcggaa gatttacgtt tgctgtatgt ggcattaacc
    10981 cgcgcggaat ctcagcttaa cttagtgttg ccgaaagaga tttccggctg gtctccactc
    11041 cattacttgt tagcagacgg ggaaatacct acaggaaaag tgcctgtttc agattatttt
    11101 agccaaaaac ggattgctgc taatttggtt gaagtagccg gggaattacc tttctcactg
    11161 tgggaaggag aacaatcttc agacgattac caagcaaaaa cctttagtgg caatatccgt
    11221 gtggttggtc aaattaccag ctttacttca ctttatgccc aacacgagcg ttttagtgaa
    11281 gggaaatcag aaagggtaat ggatttagcc caagatcgag atgtacatat agaaaccatt
    11341 gaggcaggtt attcaagtga aactgccgct ttctcgccat tccaattccc acacggtatt
    11401 aaagtgggca cggtgttaca tcactttttt gagcattgcc aatttaacga acaaattgat
    11461 agagaggctg ttgctaaagt atgcgagcaa ttagggctaa gcgaggaatg gattgaacca
    11521 acagcattat ggtttgagcg tattctaact acccctttgg cagaggcaaa tttctgctta
    11581 aaagcgattg atgaaaccaa acgattaaac gagtggcaat tctatttacg cttaaaaaat
    11641 gataaagcct tacatcaatt aaatgcgtta ttaaagcaac atagcccttt agcaaaaact
    11701 ttacctgaat tgcaattacc gcaattagaa ggttttgtgc gaggctttgt agattgtatc
    11761 gtgcaagtgg aggagaaatt ctacttaatt gattacaaat cgaatttctt aggctatctg
    11821 ccacaaaatt atgcgaaaga acacattcaa cgggaaatgg gcagacaacg ctatgatttg
    11881 caatatctgc tttacactct cgccttacac cgttatctaa ctgcccgttt aggtgaaaaa
    11941 tacgattatg aacgagactt cggcggagtg gcttacttgt tcttaagggc aatgaacggc
    12001 gaaaatgata acggcgttta ttttgacaag ccgactaagc agttgattga agaaatggat
    12061 cggttgtttg ggtaaatatg tgtaggggcg aaaatttttc gcccctttat gggtaattaa
    12121 ctcgcttgct cataccaaat cttattgata taaagcaaca ctttaccaag cggagtttct
    12181 accctaattt catcatcaac ctgtttgccg attaaggctc gggcgacagg ggagtcaatc
    12241 gaaatccagt tttttgctgg gtcgaactca tcgcaaccaa caatacggta ctgttttgcc
    12301 tcgccctctt cattttctaa ttccacccaa gcgccgaaaa agactttgcc ttcttgttta
    12361 gggtgataat caacaatttg tagcacctct agccgcttag ataaaaaacg cacacggcgg
    12421 tcgatttctc tcaaacggcg tttgccgtaa atatattcgg cattttcgct tcgatcaccc
    12481 aatgcggcag catcagacac cgcttgagtc actttcgggc gttcttcccg ccatagaaat
    12541 tttaattcct gatctaaggc ttgccagcct gaacgggtaa tgtaatttga tttagccata
    12601 gttatttaat tgtttatgga gcaaatagtg atttgcccct acgaagtttc tattttgaaa
    12661 gcaataaatt cgctactgtt cttattccca gtccggttgc tcctgccgcc cataaatcgc
    12721 tcgccgtttt gcggaatgtg gcagaggcgt caatatgtag ccagttttgc tggtagtttt
    12781 ctacaaagta agataaaaat gcggtggcgg tacttgcccc agcccccacc ggcacagagc
    12841 cgatattggc gatgtctgca aaggaagaag caatttgccc acggtgaaat tcttcaaacg
    12901 gtaaacgcca gaacggttca ttttccgttt tggcagactt aaatagtgct tcggttaggt
    12961 tgtcgtccat tgaaagcacc gagtggtagt cattgccgac tgcgactttt gctgctcctg
    13021 tgagggttgc agcatcaata ataaattgtg gattttgctt gcacgcatca attaaaccat
    13081 cggcaagcac taaacgccct tcagcatcgg tgtttaaaac ttcaacagtt acgccattgc
    13141 ggtaagtgat aatatcgcct aatttgaacg cattgtggct gaccatattt tccgcacagc
    13201 agaggtagag tttcactcgt ttattcaagc ctcgagcaat ggcaaaagca agtgagccgg
    13261 ttaaaagtgc cgctccgccc atatcggttc gcattgtgct catactatcg cttggtttta
    13321 gactataacc gccggtgtca aaggtaatgc ctttgccgac caagcaagcc agtacctgtg
    13381 cgttttcatc gttggttggg ttgaagtcta atactaagaa tgcaggcgga ttggcagagc
    13441 cttttccaac tgtccaaatg ccgtgatagc cttgttcttg taatgcttcg ccacgaatga
    13501 tctggtaaga aaccgtattc ggttttgcca ttttttggat aaactcgacc gcttgttcgg
    13561 ctaatttttc aggggttaat ttatctgatg gcttattaat taattcacgc acgaaagtgg
    13621 cacattttac acggttatca aattcggctt gctgttcgtc taagtctggg tattgtaccg
    13681 agccttgatt ttttaccgaa tgaaatcctt gatgaaacgc ccagcaggat tctaaatccc
    13741 aactatcgcc ggtcagctcc acggttgaaa taccttgatt tttaattttg cgggcggctt
    13801 tttgaatagt ggctaaatcg ttgttcggat tatgtacggt aaaccccgca tcatttgcgg
    13861 aaagaatagc atttttaccc caaatttcag gggcgttttg agtagaaagt gaaattttca
    13921 ttgctgtcat tatcggtttc cttagaaaat ggattttgct ttatcctaac aaacttttgc
    13981 aaagttttat acaaaaatga ccgcttgcag agcatagcct tgacaagcgg tcattgtttc
    14041 gtgtttttac aacctgatta aggacggatt atttgccgtc ccaagcttta attgcattgt
    14101 ggcggatttt aacacggctt tcagcattgc ctttgtagta atctttatct aaaccgcctt
    14161 cccagtcgcc atagttcggg ttcggtaaca taatgaattt tttaccgaat tgttggctgt
    14221 tttcggctac gaatgcacgg cgttccgcgt ttgatttacg gtaagtcgca tcgccgaaat
    14281 cgtttaagtt gtcgccaata tagagcacga tttcatagcc ttgtttttcg atttcttcaa
    14341 agcgaggggt tttgttcgat ttgtcttttt tcaacaagag tgtttgttcg ctcacaccac
    14401 taaagcctaa tttgttcata tcatcaatag taccggcttt ttcactgctg tctttacggt
    14461 ttgaaacata gaacatagtt cctttgtggc tattaacgta gttgttaaat tccactgcac
    14521 cggcaatcgc tccggtttgg cgggcattta cccaacgagt ccagctttca ccatcaaacg
    14581 gcttgtggtt tttaacctgc cagccggcat aaaggctgtt gtccaccata gtttcatcta
    14641 aatctactac cacggctttt ttcttgcctt ttttcacttt ggcttggtcg aatgccactt
    14701 tcgctgcatt gaatgcttga tgggcaagag cttggtattc gccggattgt tgcatccagt
    14761 ttatccctaa tgtagcttgc tcttgcaaca ccatttgatg atgctcatcg ctgtttttat
    14821 gcgtgtgcga acaagcagtt aatacaaata aagctgtgag gctcatagca gaaagtttga
    14881 tgtttttcat acgtatatcc ttagttttat tgttgataaa cagagcgatt atacctaaaa
    14941 agagttgctt atggctaata gtttattgct cctattcact tttaaagcaa actaaggtag
    15001 cctagtaacc tttttataac aattacacag aggctatatg caaacattat taaaatttac
    15061 acaatttatc agcaaaacct tcgccatttg ggtggtgttt tttgctttta tcgcagcaca
    15121 atttccggaa acctttaagc aattcctgcc ttggattcct tatctgttag ggattgtgat
    15181 gttgggaatg gggttaacct taactttcaa agattttgcc gaggtaacga aaaatccgaa
    15241 atcagtgatt atcggcgtga tcgctcaatt cgtggtatgc cgagtgtagc ttatggattg
    15301 gcaaaagcat ttaacttacc cgcagattta gccattggtg tgattttagt tggttgttgc
    15361 ccgggcggta cttcttcaaa cgtgatgacc tatttggctc gtggcaacac agcgttatcg
    15421 gtagcttgta ccactatttc aactttactt gccccagtgc tcacgccggc aattttctat
    15481 ttatttgcca gccaatggtt agaaattaat gcaacggcaa tgtttatttc ggtgttgcaa
    15541 atggtgctat tgccaatttt tgtgggcgtg gtgattcgta ctattttcaa acaaaaagta
    15601 gagcaattta gccaaacaat gccattaatt tcagtgattg caatcgtatt aattgtcacg
    15661 gcggtggtgt cagttagccg agatcgtatt attgaatccg gtttattaat ttttgcggta
    15721 gtagcattac acaacggttt aggttattta atcggttatt tggcgagccg tttattaaaa
    15781 ttaccgattg cagacagcaa agcggtggcg attgaagtgg ggatgcaaaa ctcaggattg
    15841 ggggcagcac ttgccgcatt acactttaaa gccaacccta tcattgcggt gccaagtgcg
    15901 gtatttagct tctggcacaa tatttcaggc ccgattttgg cgatgatttt cgctaaaatg
    15961 aaaaacgata acgaaaaata aaattcgttt taatacagac gttttaatgt tttaataaag
    16021 aatagacaaa cggttaaatt tttctaattt tttgcaaaat gttcagaaaa tttaaccgct
    16081 tgttgtttag aatttagaac attcttggtc cacgtgcagt gctgattttt tcatcatccg
    16141 ctctttgggt aacaatcact tgatcaccct catttaagcc tgctaatact tcggtatttt
    16201 gcgtatccga aatgccaatt tggatttttc tttcaaccga ctgctcattt tccagcacca
    16261 acacataatt ctctttacca cgttttttga ttacgctggt gggtacaact aaaacctgtt
    16321 cccgtttatc aatagtaatt tgggcttgag tagtcattcc gatgcgtaaa ctgttatcgg
    16381 cattatcaac ggaaacatta gcgtagtaat aaaccgcttc ggtattaccc gattgttcgg
    16441 tgtagttatt atcggtcagc gtggtgagtg ccggatcaac tgaatcaatg gtagcactat
    16501 attctcgatt tggctcagca agggtggaaa acttaacttt ttgcccgact ttaacctgtg
    16561 caatatcgcc ctccgaaatt tcaagtttaa tcagcatctt agataaatct gctacctgta
    16621 caatagttgg cgaagtttga ttggaattga ccgtttgccc gacagaaacc ggtacagaca
    16681 caatcacgcc gtccattggc gagataattt gcgtataagt taagttggtt ttagcatcgt
    16741 ttaccgaaat ttcagccgat tttaattgtg ctttaatgtt atccaccgtg gctttggctg
    16801 aagctaaggt atttttagcc gtttcaagat cgtttaaaga ggcactattt gcccgataga
    16861 gtttatttaa gcggttgtag ttcgattgag ctacttcaag tgcaacctgt gcagaggtga
    16921 gctgtgcttt ataagaagaa agccgggctt gagcggtgct taaggtgttg ctctggttgc
    16981 tggaatcaat atctgcaatt aaatctcctt gcttcaccga ttgcccgagc tcgacataga
    17041 gtttttggat tttgcccgaa acttgagccc ccacttcggt gcgtttattc gctcgaaccg
    17101 aaccggttgc cagcacattt ttgttaattt ctgcccgtgt gacagcttgg gtaatgtaat
    17161 ggatttgcgg tttattggtt tgttgctgat aataaaaata gccgcctatt ccgagagcgg
    17221 caattagcac aagagtggct aatgttttac gtttcatact gttccttgat gatatacaag
    17281 cggtcaaatt tagacaaaaa tttgcaaaaa agttgctctc aactaaacaa tgccaaatgt
    17341 ggctcgaaga tagcttgcca ccgcttcatc tttgttaaag ccaatcactt cattattcgg
    17401 taatacggct tttaaacgtg gatcggcatt acccattaaa caacctttgc cggctaaact
    17461 tagcatttcc acatcattca taccgtcacc aaaagcgata caatcggcta aggtgtagcc
    17521 ccgtaatttg accaattcgg cgagagcatt ggctttacac actccttttg ccatcacttc
    17581 aaggcaaagt agggcagagt aggtcatttg cacacggtcg ccataggtat cccgaatata
    17641 atgttcaatc ggtactaaag cttcagcagt tttgccgata aaaaacactt tctcggtatt
    17701 tcgaccgtga tgatgagcaa aatttactac ttgatagcta aatcctgaat cttgatggaa
    17761 tttttttaac tctgcaatct ctttattgat aaaccattca tcgccttgat agctgtttaa
    17821 gcacacattt tggtcgtcaa attcaatgtt cattaattca aaggcaagat cttccggtaa
    17881 ataatggttg agaatttttt taccctctaa attgttggct cttgccccgt ttgaagtgac
    17941 taacattgca tttttaacgt ttactttccc aataatctga gacacatctg gatgattgcg
    18001 accggtggcg aaaaaaatat caacaccttt ttttgatagt tgttctaatg tatcaatagt
    18061 gaaatcacca attttatgat tggcatttaa tagtgtgcca tcaagatcgg aaataattgc
    18121 a
//
LOCUS       DANLXS010000030        17692 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.030, whole genome shotgun sequence.
ACCESSION   DANLXS010000030 DANLXS010000000
VERSION     DANLXS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17692
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.030"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..397)
                     /locus_tag="PXD71_000793"
     CDS             complement(<1..397)
                     /locus_tag="PXD71_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005752829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL6290942.1"
                     /translation="MSIAQNHNFKDKVVVITGAGGVLCAYFAKEIAKTGAKVALLDIN
                     LEAAQNFASEIADEGYIAKAYQTNVLELESIKQARDEILKDFGTCDILINGAGGNNPK
                     ATTDNEFHDINLPENTKSFFELDKAGIEFV"
     gene            complement(409..1680)
                     /locus_tag="PXD71_000794"
     CDS             complement(409..1680)
                     /locus_tag="PXD71_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL6290943.1"
                     /translation="MLWLFLGSLFLFLFLGVPVALSMLLSASFMLWKMDMFDAQLITE
                     NFVMGTNNFPLMAIPFFMLTGEIMKHGGISERIINFAMSMVGHIKGGLGYVVIISGLI
                     FAGLSGSAVADTAALGAILIPMMVARKYDVARSTGLICAAGIISVVIPPSIPMIVYGI
                     TAGASITKLFMGGMVPGFLMVLGLWLVWKFLYRNEEQTVEPKKTGKERWAAFKSAFLP
                     LLLPIIIIVGLRGGIFTPTEAGVVAAIYAAIISIILKGLTLDKIQDVFLGTIKTTAMV
                     MFVAAAAMISAFAVTVAQIPMELVEFIKGITDSPTVLMFVIMIFLLLVGCVMDLIPAV
                     LIFVPVLLPLLRAYNIDIAYFGIMMVINLSIGLITPPVGTVLYVGSGIAKIGIARLTK
                     GIYPFLLVYAAILFLLILFPEIIIVPMNWLS"
     gene            complement(1683..2162)
                     /locus_tag="PXD71_000795"
     CDS             complement(1683..2162)
                     /locus_tag="PXD71_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL6290944.1"
                     /translation="MQISTALDRTLKLLAIIALSTMIILVFFNAVLRYFFDSGIAWSE
                     EFARICFVYMIFLGIILVAKERGHLTVDIVVSALPAKVQMYLLFFADILVLVALSFIT
                     YGAYQLMLLTYTQKMPATEISSAFLYFAAVISSISYFAIVFTNLWKHIRLAFIKEEK"
     gene            complement(2223..3206)
                     /locus_tag="PXD71_000796"
     CDS             complement(2223..3206)
                     /locus_tag="PXD71_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL6290945.1"
                     /translation="MKLKTFTKALLISSLMFTGSAFAKTIIKIGHYNSDVHPSNIALQ
                     EYFKKTIEEKTEGRYEIRIFPNNQLGGEDQIVNGLRNGTIEGGITGLLLQNVDPIFGV
                     WEWPYLFKDNAEAKKVLESPIAQEIGEKMEKYGIKLLAYGMNGFRVISSNKKVENFDG
                     FKGLRLRVPLNSLFVDWAKAMHINPQSMPLSEVFTALEQKVIDGQENPYMLIKDSGLY
                     EVQKYIIESNHIFSPGLLQLSLKTWNKMSKEDQTIFLEAAKLYQEKEWELAMKMEQDV
                     KDFFHKNGKEVIVPSEQFKADMLKASETLYQNFYQKYDWAKDVISKIHAAK"
     gene            complement(3235..4260)
                     /locus_tag="PXD71_000797"
     CDS             complement(3235..4260)
                     /locus_tag="PXD71_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL6290946.1"
                     /translation="MEKPLKSVCLVEPNKVGIQTVSYPVKGENDVLIKVEGMGICGSD
                     IGAFRGTNPLVTYPRILGHEIVGTVIESGKGMPNNINVGDRVIIEPYIYCGHCYPCSI
                     GRTNCCESLKVLGVHIDGAMQEVVRHPAHMIIKTPDMPIHELALAEPLTISLHAIHRT
                     KVKAGEHVAIIGAGAIGLMAALVAKAYGATPILIDILDKRLDYAKSIGIPHTINPTKE
                     NDLEAIKSITNGRMAEVVIEASGANVAVQNTLKYTSFAGRIALTGWPKTETPLPTNLI
                     TFKELNIYGARTSKGAFEEALELLASRRIEPKDIISKVITFDEIPHYIEELSANPDDY
                     LKIIAVF"
     gene            4424..5185
                     /locus_tag="PXD71_000798"
     CDS             4424..5185
                     /locus_tag="PXD71_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HDL6290947.1"
                     /translation="MQNSSRTYTKIGKILKEEIAQGKYRVGDKLPTERDISERFEVSR
                     TIVREAIVMLEVEKLIEVKKGSGVYVIRVPELFPEDFPEEKDNELRDVGPFELLQARQ
                     LLESSIAEFAAQQATKQDIQVLRNILESEKKLLLEKMDDYSADKEFHLALAHMTGNDV
                     LIQLQEQLWRYRFNSAMWAQLHSRILQHDHHHLWIEDHERILNAIQRKNPALARKAMW
                     QHLENVKTKLLELSDVEDPKFDGYLFNVNPVVVGI"
     gene            5215..6399
                     /gene="uxuA"
                     /locus_tag="PXD71_000799"
     CDS             5215..6399
                     /gene="uxuA"
                     /locus_tag="PXD71_000799"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL6290948.1"
                     /translation="MEQAWRWYGPNDPVSLDDVRQAGATGIVTALHHIPNGQVWSVEE
                     INKRKALLAEKGLVWSVVESVPVHEEIKTQTGNYQQWIDNYKQTLRNLASCGIDTVCY
                     NFMPVLDWTRTDLEYEMPDGSKALRFDQVAFAAFELYILKRPGAEATYSSAEQAEAKA
                     YFEQMSDADIAKLTKNIIAGLPGSEEGYTLEEFQAQLDRYQGLSAEKFRTHLAYFLNE
                     IVPVAQEVGIRMAIHPDDPPRPILGLPRIVSTIEDMQWFVETQPLAANGFTFCTGSYG
                     VLAENDLVKMAEQFADRIYFAHLRATQREENPQSFHEADHLAGDVDMFGVVKALLTEE
                     YRRKANGDHRLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLRGLAEFRGLELALKKVYF
                     ND"
     gene            6450..7319
                     /locus_tag="PXD71_000800"
     CDS             6450..7319
                     /locus_tag="PXD71_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6290949.1"
                     /translation="MFKKYFGEIILFLVTFIAAAGWFFSKHALVGLPPIGFMGGRFIL
                     ASLILLPFSYRELYRLDKVQVKYSVITGLTYGIYTIFWVLGLAFSTVFGEGAFLVSLA
                     MLIAPLLSWLIFKHTPPKIFWLALPIALSGLYFLSAEKGIFHFSLGSTIFLVSSVFGA
                     LYFVLNNQYAKQVSLFPLITIQLAIVGAVCSLYSYFMESWHFPILTEVWLWFAASVLI
                     ATNLRFLMQTLGQKYCHIGTSALIMLLEPVWTLLLSVMLLGEQLSATKWLGCSLILIA
                     LIIYRLPILRLKK"
     gene            7487..7801
                     /gene="rsfS"
                     /locus_tag="PXD71_000801"
     CDS             7487..7801
                     /gene="rsfS"
                     /locus_tag="PXD71_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL6290950.1"
                     /translation="MEQNLVEFLTKTLDDLKAQDILAIDVKGKSSITDTMILATGTSS
                     RHVASTAERLITEAKQAGFEVFGDEGKAVADWIVVDFGKAMVHLLQSDSREMYQLEKL
                     WA"
     gene            7878..8345
                     /gene="rlmH"
                     /locus_tag="PXD71_000802"
     CDS             7878..8345
                     /gene="rlmH"
                     /locus_tag="PXD71_000802"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL6290951.1"
                     /translation="MKIQLIAVGTKMPAWVTTGFEEYQRRFPKEMPFELVEIPAGKRG
                     KNADTKRILELEGKAMLAAAGKAKIVTLDIPGKAWTTEQLATQLEGWKNDGRDVALLI
                     GGPEGLSPECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTINHPYHRE"
     gene            8369..10318
                     /gene="mrdA"
                     /locus_tag="PXD71_000803"
     CDS             8369..10318
                     /gene="mrdA"
                     /locus_tag="PXD71_000803"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL6290952.1"
                     /translation="MFGKLAKFTASNQREKVRDGQAESNLYARRALVAFLGVLALSGV
                     LLTNLYHLQVVNYEDYQTRSNGNRIKLLPVPPARGLIYDRNGKVLAENLTYFGLFIVP
                     EKTENIEQTLTELKDIVGLTDEDIENYHKEKKRSSRYTPIMLKGNLNEAQMARFAVNQ
                     YRFPSLDVQAYYKRNYPYGELLTHIIGYVAKINENDKKKLQEAGKFGNYAGSHDMGKL
                     GIEKFYEDQLHGVTGFEEVEINNRGKVIRKLRDQAGVAGSSIRLSIDVELQQYVMSLI
                     AKHKGAVVVLDPRDSSILAMVSNPSYDNNLFVGGISGQDYKALLDDPTRPLYSRTTQG
                     TYPPASTIKPFMAVAGLEEGMITPSTTISDPGYWILPGTTQRYRDWKRSGHGATNVNK
                     AITESSDTFFYQLAYKMGIDKMSDWMKKFGFGVKTGIDIFEESAGVMPTREWKQKRYK
                     KPWVQGDTIPVGIGQGYWISTPLQLAKATAVLINNGRVNTPHLMLEVQSSNSVTPYQD
                     PLLYEDIKGVPDVYWNLAKQGMYNVNHAANGTARKVFAGAFYHSAGKTGTAQVFNLNG
                     KNYDKNAIKKELHDHAWFIGYAPYEQPKIVVALILENAGGGGSAAAPVARQIMDYALR
                     KGIGEQPDSQNSEQNKPLVTEEHHE"
     gene            10311..11438
                     /gene="rodA"
                     /locus_tag="PXD71_000804"
     CDS             10311..11438
                     /gene="rodA"
                     /locus_tag="PXD71_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDL6290953.1"
                     /translation="MNSGFKKLFWKIFSLDLLLLSGLLAITGYGLLVLYSASGANERM
                     FTSRLIQVSLGLGLMLIMAMVPPRFYQKVSPYLYIFCLILLILVDLVGETSKGAQRWL
                     NLGFVRFQPSEIAKLAVPLMVATYLGKRPLPPSFKDTFIALAIIIVPTLLVAIQPDLG
                     TSILVCSAGIFVLFLAGLSWKLIGAGVVFLAGFIPIMWFFLMHDYQKTRVMTLIDPNK
                     DPLGAGYHIIQSKIAIGSGGINGKGWMEGTQSQLEFLPEPHTDFIFAVLSEEHGMIGV
                     LILLAIYLFIIARGLVIGTKSDSAFGRILSGGTALLFFVYVFVNIGMVSGILPVVGVP
                     LPLFSYGGTSYVTLMAAFGLMMSTYVHRKRVGSNNPYDKLK"
     gene            11530..12189
                     /locus_tag="PXD71_000805"
     CDS             11530..12189
                     /locus_tag="PXD71_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HDL6290954.1"
                     /translation="MRLGKIVTLLLAGFLTFSSLNSLAATKNTKAKSSILAKKQATSK
                     AIKKVGISKVKKKEVVAKATKKKTISTVTTKPIKSTKKLARKVVKKTVTAKKKLVKKQ
                     HKPLVKKHFQTGVASYYANYFNGRRTANGETFNNAKMTAAHRTLPFGTLIEVTNLRNG
                     RSVVVRVNDRGPYAHARVLDLSSAAAKKIGMHHSGTARVKIAIVDKSKLGSTNQFYVM
                     N"
     gene            12203..13381
                     /locus_tag="PXD71_000806"
     CDS             12203..13381
                     /locus_tag="PXD71_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL6290955.1"
                     /translation="MKKTLVKAMSVVGFAVSSIAYADLNTNFNLSAPQINAQTYILMD
                     YNSGEVLVSLNPDQRQYPASLTKMMTSYVVGEALKSGKVQNSDMVIVTENSWAQKFPG
                     SSLMFLDLNSKVSVGELMKGLIIVSGNDASVALAEHVSGSQAAFINEMNKYAQQFGLK
                     NTHFTTVHGLDDPEQYSSARDMAIIGSHIIRDQPEEYKIYAEKEFQYNIKKPQPNRNG
                     LLWDKTIQVDGMKTGHTDKAGYNLVASAINPNTRLISVVMGVPTYKGREVESKKLLQW
                     GFANFETIKSIPAAQSVAEQSVYYGEVNKVQLGSVQDSFVTIPKGRSADLKARYELEK
                     KNLEAPLAKGQVVGKMIYQLDGKDVASTNLQVLQDVPEAGIFGKAWDWIALTVKSLFD
                     "
     gene            13549..13848
                     /locus_tag="PXD71_000807"
     CDS             13549..13848
                     /locus_tag="PXD71_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDL6290956.1"
                     /translation="MSQPKAINLQDLPQAKLKDLLEFPCHFTFKVVGANREDLVDDVV
                     VVTQKYAKGDYNPRQQKSSKGTYNSVSIDIVAENIEQVETLYTELAKINGVRMVL"
     gene            13910..14563
                     /gene="lipB"
                     /locus_tag="PXD71_000808"
     CDS             13910..14563
                     /gene="lipB"
                     /locus_tag="PXD71_000808"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDL6290957.1"
                     /translation="MKLIIRQLGIQPYEEIWHQMQSFTDSRDGNTDDEIWLVQHPAVF
                     TQGSAGKPEHLLNPTNIPVVQSDRGGQITYHGEGQQIMYVLIDIKRLKSLGKEVSVRD
                     LVTALEQTVVQTLAEYGIESYPKADAPGVYVDGKKICSLGLRIRKGCSFHGLALNINM
                     DLTPFQNINPCGYAGLEMCQLSELVHRPDLRCEEVAPKLVAHFSTILGYNNQQIINN"
     gene            14578..15585
                     /gene="lipA"
                     /locus_tag="PXD71_000809"
     CDS             14578..15585
                     /gene="lipA"
                     /locus_tag="PXD71_000809"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDL6290958.1"
                     /translation="MATSTAQTTATATVKKMSPFKMEKGVKYRDAAKTSVIQVRNIDP
                     DQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFNHGTATFM
                     IMGAICTRRCPFCDVAHGKPLPLDPDEPRKVAETVQDMKLKYVVITSVDRDDLPDRGA
                     AHFSATVREIKALNPHCKVEILVPDFRGRVEQAVAILKENPPDVFNHNLENVPRLYRE
                     VRPGADYKWSLELLKIFKQEFPHIPTKSGLMVGLGETNEEILEVMQDLRDHGVTMLTI
                     GQYLQPSRHHLKVERYVPPAEFDMFREEAEKMGFEHAACGPFVRSSYHADLQAKGELV
                     K"
     gene            15772..16875
                     /locus_tag="PXD71_000810"
     CDS             15772..16875
                     /locus_tag="PXD71_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6290959.1"
                     /translation="MKKLLLNSVFAATLLAFGATANAEIKTITDVLGREVKVDVPAKR
                     VALTFYYPDYIAVTGVENFDKVVGISREFWEKFNPGSWALFAEKMPNLKNIADLGYVN
                     SGTFSTEKTIALKPDVLVLPEIQYQALASEISRIEQAGIPVVVDFNKQTIENHTKSVR
                     VFGQLTGTEERAEKIAKEYEEGIADIQSCIDNAKVSKPKIYAEFGNKGPKEHSFTFGK
                     NMWGAIAETVRGDNISAPFVENWGQINPEQVLVSKPDVIMISGTELGNDTNPEIMSMG
                     INIEEADAQKRLKGFIERTGWKELPAVKSGRVYGLYHTASRSISDLASAQFMAKALYP
                     ELFKDIDPEKTYLDFHNNYLPITPKGTFFMQLK"
     gene            complement(16966..>17692)
                     /locus_tag="PXD71_000811"
     CDS             complement(16966..>17692)
                     /locus_tag="PXD71_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL6290960.1"
                     /translation="LVVADKINLHFEQGKIYTLLGPNGAGKSSLLKTLFGEIAHQGTI
                     DFDGNKLTSSHLHSWRKEIGYMPQDTQVEASLSALEVILLGQLDSLNMYISDELLNEA
                     VDIMDKLGISHLAHQDIMRLSGGQRQMVMFAQVLLRKPHILLLDEPVSALDMYHQINL
                     LEYVNAYTRANNLITIMVLHDLSLAAQFSDSVMLLSEGKFKHKAELVICWNAILFRDS
                     IMSILRFYMMETARRLSARFANV"
BASE COUNT         5356 a         3310 c         3823 g         5203 t
ORIGIN      
        1 atacaaattc aatccctgct ttatctaatt caaaaaaaga ttttgtattt tccggtaagt
       61 taatatcgtg aaattcatta tctgtcgttg cttttggatt attcccacct gcaccattaa
      121 ttaaaatatc acaagtgcca aaatctttta gaatttcatc tcttgcctgt tttatgcttt
      181 ctaattcaag tacattagtt tggtacgctt tcgcaatata gccttcatcg gcaatttcag
      241 aagcaaaatt ctgtgctgct tctagattga tatcgagtag tgcaactttt gcacctgttt
      301 ttgcaatttc ttttgcaaaa taagcacata gcacaccacc tgctccggta ataacaacaa
      361 ctttatcttt aaaattatga ttttgggcaa tagacataat aagaactcct aagataacca
      421 attcattggt acgataataa tttcagggaa aagtattaat aagaacagta ttgcagcata
      481 gactagcaag aatggataga tacctttggt taatctagca ataccaattt ttgctatacc
      541 agaaccaaca tataataccg ttccaacagg aggcgtaatt aaaccgatag ataggttaat
      601 taccatcata ataccaaagt aagcaatatc gatgttatac gctcttagta atggtaatag
      661 tactggtaca aaaattaata ctgctgggat taagtccatt acacagccga ctaataataa
      721 gaaaatcata atgacgaaca ttagtacagt cgggctatcg gtaatacctt tgataaactc
      781 aactaactcc ataggtattt gagcaacagt aactgcaaat gcagatatca ttgccgccgc
      841 tgcaacaaac ataaccattg cagttgtttt gatagtgcct aagaaaacat cttggatttt
      901 atctaaggtt aaccctttta agataatgct gattattgct gcataaattg ctgctacaac
      961 tcccgcttct gttggtgtaa agatgccgcc tcttaaacct acgataataa taattggaag
     1021 tagcaatggt aagaatgctg atttaaatgc agcccaacgt tctttacctg tttttttcgg
     1081 ttcaacggtt tgttcttcgt ttcggtataa gaatttccaa actaaccata aacctaaaac
     1141 cattaaaaag cctggaacca ttcctcccat aaataatttg gtaattgatg caccggctgt
     1201 aataccatag acaatcattg gaatgcttgg tgggataact acagaaataa tacctgctgc
     1261 acagattaag cctgttgaac gtgcaacatc atattttctt gcaaccatca tcggaataag
     1321 aattgcaccg agagccgcgg tatcagcaac agcggagccg gataaacctg cgaaaattaa
     1381 gcctgaaata atcactacat agcccaatcc gcctttaata tggccgacca tgctcattgc
     1441 aaaattgata atacgttcgg aaataccgcc gtgtttcata atttcaccgg tcagcataaa
     1501 aaacgggata gccatcagcg gaaaattatt tgtacccatt acgaaattct ccgtgattaa
     1561 ttgggcatca aacatatcca ttttccatag cataaaactg gcactaagta gcatagagag
     1621 agcaacgggc actcctaaaa agagaaagag gaatagactt cctaaaaata gccataacat
     1681 atttatttct cctctttaat gaaagctagc cgaatatgtt tccataaatt tgtgaataca
     1741 atagcgaaat aggagataga tgaaataact gctgcaaaat atagaaacgc agatgaaatt
     1801 tcagttgctg gcattttttg tgtgtatgtc aataacatta actggtatgc accgtaagta
     1861 ataaatgaca gtgcaactaa gactaaaata tcagcaaaga acagtaagta catttgtact
     1921 tttgccggta gtgcagaaac aacaatatca acggtgaggt gtcccctttc ctttgcaacg
     1981 aggattattc ctagaaagat catatagaca aagcagattc tggcgaattc ctcagaccaa
     2041 gctattccgg agtcaaaaaa atatcgtaaa accgcattaa aaaagactag gataatcatt
     2101 gtagatagag caattatcgc aaggagtttg agagttctat ctaatgctgt agaaatttgc
     2161 ataaatttct ccttcttcat ttagatgggc gacctctttt atccggtcgc ctcgcttatt
     2221 gcttatttgg ctgcgtggat ttttgaaata acatctttag cccaatcata tttctgatag
     2281 aaattttgat aaagcgtttc ggatgctttt aacatatcag ctttaaattg ttcactcgga
     2341 acaatgactt ctttgccatt tttgtggaag aaatctttta catcttgttc catcttcatc
     2401 gctaattccc actctttttc ttggtagagt tttgctgcct ctaagaagat tgtctgatct
     2461 tctttagaca ttttattcca agttttcaaa ctaagttgta gtaagccagg tgagaaaata
     2521 tgattagatt caataatata tttttgcacc tcgtataaac cagaatcttt aattaacata
     2581 tatggatttt cttgcccatc aatgactttt tgttctagtg cggtaaatac ttcacttaaa
     2641 ggcatacttt gagggttaat atgcattgct tttgcccagt ccacaaacaa cgaatttagt
     2701 ggaaccctta atcgcaaacc tttaaatcca tcaaaattct caactttttt attggaagag
     2761 ataacacgga agccattcat accatacgct agtaatttaa tgccgtactt ctccattttt
     2821 tccccgattt cttgagcaat aggagattct agaactttct tagcttcggc attatcttta
     2881 aatagatatg gccactccca tacgccaaaa attggatcaa cattttgtag taataaacct
     2941 gtaatgccac cttcaatcgt gccattacgc aaaccattta caatttgatc ttctcctccc
     3001 aactggttat ttgggaaaat acggatttcg tagcgtcctt ctgttttttc ttcaatggtt
     3061 tttttgaagt attcctgcaa tgcgatattt gatggatgaa catctgaatt atagtgacca
     3121 atcttaatga tagtcttcgc aaatgccgaa ccggtgaaca tcaggctaga aattaataga
     3181 gctttagtga aagttttgag tttcatagtt attacctcgt aggtgagtta gtatttaaaa
     3241 tacagcgatg atttttaagt aatcatcagg attagctgag agttcttcga tatagtgagg
     3301 tatttcatca aatgtaatta ccttactaat gatatctttt ggttcaattc ttcttgatgc
     3361 cagaagctct aatgcttctt caaatgcccc cttgcttgtt cttgccccat aaatattaag
     3421 ttctttgaag gtgattaaat tagttggtaa tggtgtttct gttttcggcc atccggttaa
     3481 agcaatacgt ccagcaaaag aggtgtattt aagcgtattt tgtactgcga catttgctcc
     3541 tgacgcttca ataaccacct ctgccattct tccattggta attgatttaa tagcttcaag
     3601 atcgttttct tttgttggat taattgtgtg tggaatacca atagattttg cgtaatccaa
     3661 tcgcttatct aaaatgtcaa ttaaaatcgg cgtcgctcca taagctttgg caaccaatgc
     3721 agccattagc ccaattgccc ctgctccaat gatagcaaca tgttcaccag ctttgacttt
     3781 tgttctatga attgcgtgta aggaaatagt taagggttct gctaacgcta attcgtgtat
     3841 tggcatatca ggtgttttga ttatcatatg ggctggatgt ctaacaacct cttgcattgc
     3901 cccatcaata tgtaccccca atacttttag agattcgcaa caatttgttc taccaatgga
     3961 gcagggatag caatgtccac aatagatgta aggttcaatg atgacacgat ctcctacatt
     4021 aatattattt ggcatacctt taccagactc aattactgtg ccaacgattt catgccctaa
     4081 aattctagga taagtaacaa gtggattagt gcctctaaat gctccaatat cagagccaca
     4141 tatccccata ccttctactt tgattagcac atcattttca cctttaacag gataagaaac
     4201 ggtttgtata ccaactttat taggttctac aagacatact gatttgagtg gtttttccat
     4261 gattttgtcc ttttttgata aagttaatcg acattttgta gtgtcaatgg tattttatgc
     4321 acagatacta acaccaaaaa acaagtttgg cattccaatt tttgtgattt tgtgaacctg
     4381 atcacaaaaa tgaaattttg gaattccaat ttcgtgagga tttatgcaaa atagttccag
     4441 aacttacact aaaataggaa aaattctcaa agaggaaatt gctcaaggaa aatacagagt
     4501 aggagataag ttacctactg agagagatat ttctgaaaga tttgaagtga gtagaactat
     4561 cgtaagggag gcgatagtga tgctagaagt agaaaagctt attgaggtaa aaaaaggatc
     4621 gggcgtgtat gtcattagag tcccggagct ttttccagaa gattttccag aagagaaaga
     4681 taatgagcta agggatgttg ggccatttga actacttcaa gcacgacaat tattagaaag
     4741 cagtattgct gagtttgcag cacagcaggc taccaaacaa gatatacaag tacttcgtaa
     4801 catattagaa agtgagaaga aacttttgtt agagaagatg gatgactaca gtgcagataa
     4861 ggaatttcat cttgcattag ctcatatgac gggtaacgat gttctgattc aattacaaga
     4921 acagctttgg cgataccgct ttaatagtgc gatgtgggct cagctacact ccagaatttt
     4981 acagcatgat catcaccact tgtggataga ggatcatgag cgaattttaa atgcaataca
     5041 gagaaaaaat cctgcattag ctcgtaaagc aatgtggcaa catctagaaa atgttaaaac
     5101 taaattgctt gaattgtctg atgtagagga tcctaaattt gatggatatc tattcaatgt
     5161 aaaccccgtt gtagtaggta tctaaaacct aaactacaca atgaggagtt tactatggaa
     5221 caagcatggc gttggtatgg accaaacgat ccggtatctt tagatgatgt aagacaagca
     5281 ggggcaacgg ggattgtgac agcgttgcac catattccca acgggcaggt gtggtcggtt
     5341 gaggaaatta ataaacgtaa ggcattatta gctgaaaaag ggctggtttg gtcggtggta
     5401 gaaagtgtgc ctgttcacga agaaatcaaa actcaaacag gcaactatca gcaatggatt
     5461 gataactata agcaaacctt gcgtaatttg gcaagctgtg ggattgatac ggtgtgctat
     5521 aactttatgc cggtgctaga ttggactcgt accgatctgg agtatgaaat gcctgatggc
     5581 tcaaaagcat tgcgttttga ccaagtggcg tttgcggcgt ttgagttata tatcttaaaa
     5641 cgcccgggag cggaagccac ttactcatca gcagaacaag ccgaagccaa agcctatttt
     5701 gagcagatga gtgacgctga tattgcaaaa ttaaccaaaa atatcattgc cggtttgccg
     5761 gggtctgagg aaggctatac gctcgaagaa ttccaagcac agcttgaccg ctatcaaggg
     5821 ctctcggctg aaaaattccg tacccattta gcctatttct taaatgagat tgtgccggtt
     5881 gcacaagagg tgggcattcg tatggcgatt caccctgacg atccgcctcg cccgatttta
     5941 ggcttgccac gcattgtttc aaccatagaa gatatgcaat ggtttgtgga aactcaaccg
     6001 cttgcagcca atggcttcac gttctgtacc ggttcatacg gcgtattagc cgagaatgat
     6061 ttggtcaaaa tggcagagca gtttgcagat cgcatttatt ttgctcactt acgagctacc
     6121 caacgtgaag aaaatccaca aagtttccac gaagcggatc atcttgccgg tgatgtcgat
     6181 atgtttggcg tggtgaaagc attacttacc gaagagtatc gccgcaaggc aaatggcgat
     6241 caccgattaa ttccaatgcg tccggatcac ggccatcaaa tgctcgatga tctgaagaaa
     6301 aaaaccaacc caggctattc tgcgattggt cgtttaagag gattagcgga gttccgtgga
     6361 ttggagttgg cacttaagaa agtctatttt aacgattagc cttatttatt ataatgccct
     6421 ctactgtaga gggcatttta tagtttgata tgttcaaaaa atatttcggc gaaataatcc
     6481 tttttctcgt tacctttatt gctgcagcgg ggtggttttt ctctaaacac gctttagttg
     6541 gattaccgcc tattggcttt atgggagggc gttttatatt agcctctttg attcttctgc
     6601 ctttttctta ccgagagctg tatcgtttag ataaggttca agttaaatat tcggtgatta
     6661 ccggtcttac ttacggaatt tatactattt tttgggtatt ggggctggct tttagtacag
     6721 tgtttggtga aggggctttt ttggtgagcc ttgcgatgct gattgctcct ttgctctctt
     6781 ggttgatatt caaacatacg ccaccgaaga ttttttggtt agccttaccc attgcattaa
     6841 gtgggcttta ctttctttct gcggagaaag gcatctttca tttttcctta ggcagtacga
     6901 tttttctggt gtcatcagtg tttggggcgt tatattttgt gttaaataat caatacgcca
     6961 aacaagtctc attgtttcca ttaattacta tccaattggc gatagtcggt gcggtctgta
     7021 gcttgtattc ctattttatg gaaagttggc atttcccgat tctcaccgaa gtgtggctct
     7081 ggtttgccgc aagtgtattg attgcgacaa atttacgttt tttaatgcaa acactggggc
     7141 aaaaatattg ccatattggt acttctgcac tcattatgtt acttgagccg gtttggacat
     7201 tgttattgag cgtgatgtta ttgggggaac aacttagtgc gacaaaatgg ctaggttgca
     7261 gcttgatttt gatagcattg attatttatc gactccctat tctacgactc aaaaaatagg
     7321 taaaacaaaa gcaaatatgc catagtattg gaagcagtta ggcattttag agtaagtaac
     7381 aagcggtcat ttttgcaaaa aatcttgcaa aaatgaccgc tttctttctt ttatccgttc
     7441 aatctatgtt aaaattctac atttttataa gatgagagag agaaacgtgg aacagaattt
     7501 agtcgaattt ttaaccaaaa ccttagatga tttaaaggca caagatattt tagccattga
     7561 tgtgaaaggg aaatctagca ttactgatac gatgatttta gccaccggta catcgagccg
     7621 tcacgttgct tcaacggcag agcgtttaat tacggaagca aaacaggctg ggttcgaggt
     7681 atttggagat gaaggtaaag cagttgccga ttggatcgtg gtcgattttg ggaaggcaat
     7741 ggttcacttg ttgcaatccg atagccgtga gatgtaccaa ttagagaaac tttgggcgta
     7801 gcagttcaat aaggaacgac aagcggtgat ttttgcaaca ttttttgcaa attaaaccgc
     7861 ttgtaagtga aagagagatg aaaattcaat taattgcggt tggcactaaa atgccggcgt
     7921 gggtaacgac agggtttgaa gagtatcaac gccgttttcc gaaagagatg ccgtttgagt
     7981 tggtggaaat tccggcagga aaacggggca agaatgctga tactaaacgc attcttgagc
     8041 ttgaaggcaa agcaatgttg gcggcagcgg gcaaggcaaa aatcgtgact ttggatattc
     8101 cgggcaaagc gtggacaacc gagcagttag ccactcagtt agagggctgg aaaaacgatg
     8161 ggcgagatgt agcgttattg attggcggcc cggaaggatt atcaccggaa tgcaaagccg
     8221 ctgccgagca gagttggtcg ttgtcgcctt taaccttacc tcacccactg gtgcgggtgg
     8281 tggtggcgga aagtttgtat cgggcgtggt cggttaccat taatcaccct tatcatcgag
     8341 agtagtgagt cattttagat agtgttatat gtttggtaaa ttagcaaaat tcacagcttc
     8401 aaaccagcga gaaaaggtgc gagacgggca ggcagaaagc aatctgtatg cccgccgtgc
     8461 cttagttgcg tttctggggg tgttggcgtt aagtggtgtt ttattaacca acttgtatca
     8521 cttgcaagtg gtgaattatg aggactacca aacccgttca aacggtaacc gcattaaatt
     8581 gttgcctgtg ccaccggctc gaggcttaat ttatgaccgt aacggtaaag tattggcgga
     8641 aaatttaacc tatttcgggc tgtttattgt gcctgaaaag acggaaaaca ttgagcagac
     8701 tttaaccgag ttgaaggata ttgttgggct gacggacgaa gatatcgaga attatcataa
     8761 agagaaaaaa cgcagttctc gctatacccc aattatgctc aaaggcaatt tgaatgaggc
     8821 tcagatggct cgttttgcgg tgaaccaata ccgttttcca agtttagatg tgcaggcgta
     8881 ttataagcgg aattatcctt acggcgagct tttaacccat attatcggtt atgtggcgaa
     8941 aattaacgaa aacgataaga aaaagctgca agaagccggg aaattcggca attatgccgg
     9001 ttcgcacgat atggggaaat tggggattga aaaattctat gaagatcagc tccacggtgt
     9061 caccggtttt gaagaggtag aaattaataa ccgagggaaa gtaatccgta aattgcgtga
     9121 ccaagcaggg gttgccggta gcagtattcg cttgagtatt gatgtggaat tacagcaata
     9181 tgtgatgtcg ttaattgcta agcataaagg ggcggtggtg gtgcttgacc ctcgtgatag
     9241 cagtatttta gcgatggtct caaacccaag ttatgacaat aatttatttg tgggcggtat
     9301 ttcagggcag gattataaag cattattgga cgatccaacc cgaccacttt atagccgtac
     9361 tacacaaggc acttatccac cggcttcaac tattaagcca tttatggcgg tggcaggttt
     9421 ggaagaggga atgattaccc cttctaccac catttcagac cccggctatt ggattttgcc
     9481 cggcactaca caacgttatc gagattggaa acgtagcgga cacggtgcaa ctaatgtgaa
     9541 taaagcaatt accgaatcgt ctgatacctt tttctatcaa ttagcctata aaatggggat
     9601 tgataaaatg tcggattgga tgaaaaaatt cggtttcgga gtgaaaaccg gtatcgatat
     9661 ttttgaggaa tccgcagggg taatgccaac ccgtgaatgg aaacaaaaac gctataaaaa
     9721 accttgggtg cagggcgata ctattccggt tgggattggg cagggctact ggatttcgac
     9781 cccgttacag ttggcgaaag ccacggcggt gttaatcaac aacgggcggg tgaatacacc
     9841 gcatttaatg cttgaagtgc aaagcagtaa ttcggttacg ccgtatcaag acccattgtt
     9901 gtatgaagat attaaaggcg tgccggatgt ctattggaat ttagcaaaac agggaatgta
     9961 taacgtaaac cacgccgcaa acggtacggc acgaaaagtg tttgccggtg cattttatca
    10021 ttcagcaggg aaaaccggta cggctcaggt gtttaactta aacggtaaaa attacgataa
    10081 aaatgcgatt aaaaaagagt tgcacgacca cgcttggttt atcggctatg ctccttatga
    10141 acaacctaaa atagtggtgg cattaatttt agaaaatgca ggcggtggtg gtagtgccgc
    10201 ggctcctgtt gctcgtcaga ttatggatta tgctttgaga aaaggcattg gtgaacagcc
    10261 ggattcgcaa aattctgagc aaaataaacc gcttgtcaca gaggaacatc atgaatagtg
    10321 gctttaaaaa acttttctgg aaaatttttt cactcgatct ccttttatta tcaggattat
    10381 tagctattac cggttatggt ttattagtac tatatagcgc aagcggtgca aatgagcgga
    10441 tgtttaccag tagactgatt caagtttcgc tagggcttgg gctaatgttg ataatggcaa
    10501 tggtacctcc tcgcttttat caaaaagttt cgccttatct gtatattttc tgtctgatat
    10561 tattgatttt ggtggatttg gtcggcgaaa ccagtaaagg agcgcaacgt tggttgaatt
    10621 taggatttgt tcgctttcag ccttctgaga ttgccaagct cgctgtgcca ctgatggtag
    10681 ccacttattt aggtaagcgg ccgcttcctc cgagttttaa agatactttt atcgccttag
    10741 ccattattat tgtgccaacc ttattggtgg caattcagcc cgatttggga acatctattt
    10801 tagtgtgttc tgccggtatt tttgttttat ttttggcagg gctaagttgg aaattaattg
    10861 gcgcaggtgt ggtgttttta gccggtttta tcccgataat gtggtttttc ttaatgcacg
    10921 attatcaaaa aacccgggtg atgaccttaa ttgatcctaa taaagacccg ttgggtgccg
    10981 gctatcatat tattcaatcc aaaattgcga taggctctgg tggcattaat ggaaaaggct
    11041 ggatggaagg aacgcaatcc cagcttgaat ttttacctga accgcatacc gattttattt
    11101 ttgcagtgtt aagcgaagag catggtatga taggtgtgct gattttatta gcgatttatt
    11161 tgtttattat tgcccgaggt ttagtgattg gcacaaaatc ggattctgct ttcgggcgta
    11221 tattatcagg tggtacagcc cttctatttt ttgtttacgt ttttgtaaat attggtatgg
    11281 tgagtggtat tttacccgta gtcggtgtac cattaccgct tttcagttac ggcggaacct
    11341 cctatgtgac gctgatggcg gcatttggtt tgatgatgtc aacctatgtt caccgtaagc
    11401 gtgtaggtag taataatcct tatgataaac tgaaataaat taaacttttg cttttttgcg
    11461 gtatcaaaaa gcagtgaaac gatcatcgtt acaaacaata acgacatcca catagaggaa
    11521 aaaacttata tgagattagg taaaattgta accctgttgt tagctggatt tctgacattt
    11581 agttcattaa attctttggc tgcgacaaag aatacaaaag ctaaatcgag tattttagcc
    11641 aagaagcaag caacttctaa agcaataaaa aaagtaggta tttctaaagt taagaaaaaa
    11701 gaagtagtgg ctaaagctac aaaaaagaaa acaatttcta ctgttacaac taagccgatt
    11761 aaatctacaa aaaagctggc tcgcaaggtt gtcaagaaaa cggtaaccgc taagaaaaaa
    11821 ttggtaaaaa agcagcataa gccactggtc aaaaaacatt tccagactgg tgttgccagc
    11881 tattatgcaa attactttaa tggtcgccga accgctaatg gggagacttt caataacgca
    11941 aaaatgacgg ctgcacatcg aactttacca tttggtactt taattgaagt gacgaattta
    12001 cgaaatggtc gttctgtggt ggtgcgtgta aatgatcgcg gaccttatgc tcacgcacgg
    12061 gtcttggatt tatcatcagc cgcagcgaaa aaaatcggta tgcaccattc ggggacggca
    12121 agagtgaaaa ttgcgattgt tgataagtca aaattgggat cgactaatca gttttatgta
    12181 atgaattaat ttaagaggga atatgaaaaa aacattagtg aaagcgatga gcgtggtagg
    12241 ttttgcggtt tcaagcattg cctatgcgga tttaaacact aacttcaatt tatcggcacc
    12301 ccagatcaac gcacaaacct atattttgat ggattacaac tccggtgaag ttttagtgag
    12361 cctaaatccg gatcaacgtc aatatcctgc atcattgacc aaaatgatga ctagctacgt
    12421 agttggtgaa gccctaaaat cgggaaaagt gcaaaacagt gatatggtga tagtcaccga
    12481 aaattcgtgg gcgcaaaaat tccccggctc ttcattaatg tttttagatt taaacagcaa
    12541 agtttcagta ggtgagttga tgaaagggct gattatcgtc tcgggtaatg atgcctctgt
    12601 ggcgcttgct gaacatgttt caggctcaca agcggcgttc attaatgaaa tgaacaaata
    12661 tgcccaacag tttggcttaa agaatactca ctttaccact gttcacggtt tagatgaccc
    12721 tgagcaatat tcttctgctc gtgatatggc gattattggt tcgcatatta ttcgtgatca
    12781 gccggaagag tataaaattt atgcggaaaa agagttccaa tataacatca aaaaaccaca
    12841 accaaaccgc aacggattat tgtgggataa aaccattcaa gttgatggta tgaaaacagg
    12901 gcatactgat aaagccggct ataacttagt cgcatcagca attaacccga atacacgttt
    12961 aatttctgta gtgatgggcg tgccaactta taaagggcgt gaagtggaaa gtaagaaatt
    13021 actgcaatgg ggctttgcga actttgaaac cattaaatct attccggcag ctcaatcggt
    13081 ggcagagcaa agtgtctatt acggtgaagt caataaagta caattaggct cggtgcaaga
    13141 tagctttgtg actatcccga aaggtcgctc ggcagattta aaagcccgtt atgagttaga
    13201 gaagaaaaac ctcgaagctc ctttagcaaa agggcaggta gtgggtaaaa tgatctacca
    13261 acttgacggc aaagatgttg caagcactaa cttacaagtc ttacaagatg taccggaagc
    13321 cggtattttc ggcaaagcgt gggattggat tgcccttact gtgaagagct tgttcgatta
    13381 atcttgttat ttgccttttt atccccatct agggagcaat gcaagcggtg tgattttgca
    13441 aaaaatttgc aaaatttcac cgcttaccgt tttagatatg aatgtataaa aagtgtagtg
    13501 gcgaaaaatt tttcgcccct cgaagaaaga atagaagaag gatcaaaaat gtctcaaccc
    13561 aaagcgataa acttacaaga tttacctcaa gccaaattaa aagatctgct agagtttccg
    13621 tgccatttca cgttcaaagt ggtgggagca aaccgagaag atttggttga tgatgtggta
    13681 gtggtcacgc aaaaatatgc caaaggcgat tacaatccac gtcagcaaaa aagctcaaaa
    13741 ggtacctata attccgtttc gattgatatt gtggcggaaa atattgagca agtagaaact
    13801 ctctacacgg aacttgccaa aattaatggc gtaagaatgg tattataatg cgaggggcga
    13861 atgacattcg ccctcaattt ttcaataaag cgatgttgga aaatagaaaa tgaaattaat
    13921 aattcgccaa ctaggcattc agccttatga agaaatttgg caccaaatgc aatctttcac
    13981 agatagccgt gatgggaata ccgatgatga gatctggctg gttcagcacc cggctgtgtt
    14041 tacgcaagga tcggcaggca aaccggagca tttgttaaat ccaaccaata ttccggtggt
    14101 gcaaagcgac cgtggcggtc aaattactta ccacggcgag gggcagcaaa ttatgtatgt
    14161 gctgattgat atcaaacggc tgaaatcctt gggtaaagaa gtgtcggttc gagatttagt
    14221 gactgccctg gagcaaaccg tggtacaaac gttggcagaa tatggcatag aaagttatcc
    14281 aaaagccgat gcacccgggg tgtatgtgga cggcaagaaa atctgctcac ttggtttacg
    14341 aattcgaaaa ggttgctctt tccacggttt ggcattaaat atcaatatgg acttaacgcc
    14401 ttttcaaaac atcaatccgt gcggttatgc agggctggaa atgtgccaat taagtgaact
    14461 ggttcatcgc cctgatttgc gttgcgaaga ggttgctcca aaattagtgg ctcatttcag
    14521 cacaatttta ggctataata accaacaaat tattaacaat taggataaaa cacagtaatg
    14581 gctacatcga cagctcaaac gacggcaact gctacagtga aaaaaatgag ccctttcaaa
    14641 atggaaaaag gggtgaaata tcgtgatgcc gccaaaacct ctgtgattca ggtgcgtaat
    14701 atcgacccgg atcaagaact cttaaaaaaa ccggagtgga tgaaaatcaa actgccggca
    14761 aattcggcaa aaattgatag cattaaaaac ggtatgcgtc gccacggctt gcactcggtg
    14821 tgcgaggaag cctcttgccc gaatttacac gaatgtttta accacggtac tgcaaccttt
    14881 atgattatgg gggcaatctg tacccgtcgt tgcccgttct gtgatgtggc acacggcaaa
    14941 ccgcttccgc ttgacccgga cgaacctcgc aaagtggcag aaacggtgca agatatgaaa
    15001 ctcaaatatg tggtgattac ctcagtggat cgtgatgatt tgcccgaccg tggtgcggct
    15061 catttctctg cgaccgtgcg tgaaatcaaa gccttaaatc cgcattgtaa ggttgaaatt
    15121 ttagtgcctg atttccgtgg acgtgtggag caagcggtgg caattctcaa agaaaatcca
    15181 cctgatgtct ttaaccacaa cctagaaaat gtgccacgtc tctatcgtga agttcgtccg
    15241 ggagcggatt acaaatggtc gttggaatta ttgaaaatct tcaagcaaga gttcccgcat
    15301 attccgacca aatccggctt aatggtaggc ttaggtgaaa ccaacgaaga aatcttggaa
    15361 gtaatgcaag atttacgaga ccacggcgtg acgatgctca ccatcgggca atacttacaa
    15421 ccaagccgcc accacttaaa agtggaacgt tatgtgccac cggcagagtt tgatatgttc
    15481 cgtgaggaag ctgaaaaaat gggctttgaa cacgcagcct gcggaccgtt cgtgcgttct
    15541 tcttaccacg cggatttaca agcgaaaggc gaattggtga aataaaatcg ataagggcga
    15601 atattatttg ccctttgttt ttgtggtttg caaagtttta gcaaaattaa accgcttgta
    15661 atctcttcta atttactctt ctatagataa tttcgttgtt gcgaattatt ttcattgact
    15721 atttgttata gaaagttaca attcaacttt ccatttcttt ttggagttcc aatgaaaaaa
    15781 ttactattaa acagcgtatt tgctgccaca ttgcttgctt ttggtgcaac tgccaatgcc
    15841 gaaattaaaa ccatcactga tgtattaggg cgtgaggtca aagttgatgt gcctgctaaa
    15901 cgtgttgcac tgactttcta ttaccctgac tatattgcgg taaccggtgt tgaaaatttc
    15961 gataaggtag tagggatttc acgagagttt tgggaaaagt tcaacccagg cagttgggca
    16021 ttatttgcag aaaaaatgcc aaatctaaaa aacattgccg atcttggcta tgtgaatagt
    16081 ggtacttttt caacagaaaa aaccatcgca ttaaaaccag atgtgttagt gctacctgaa
    16141 attcaatatc aagcgttagc cagtgaaatt tcacgcattg agcaagctgg cattcctgtt
    16201 gtagtagatt tcaacaagca aactattgaa aaccatacaa aaagcgtgag agttttcggt
    16261 cagcttacag gcactgaaga gagagcggag aaaattgcca aagagtatga agagggcatt
    16321 gctgatattc agagctgtat tgataatgca aaagtaagta agccgaaaat ctatgctgaa
    16381 tttggtaata aaggcccgaa agagcatagt tttacctttg gtaaaaatat gtggggagcg
    16441 attgccgaaa cggtgcgagg cgataacatt agtgccccgt ttgtagaaaa ctgggggcag
    16501 attaaccctg agcaggtgtt agtttctaaa cctgatgtga ttatgatttc aggtacggaa
    16561 ctaggcaacg acactaaccc tgagattatg tcgatgggga ttaatattga agaagccgat
    16621 gctcaaaaac gcttaaaagg ctttattgag cgtacaggtt ggaaagagtt acctgcggta
    16681 aaatcagggc gtgtttatgg tttatatcat acagcttctc gctcaatttc agatttagcg
    16741 agtgctcaat ttatggcaaa agcactttat cctgaattgt ttaaagatat cgatcctgaa
    16801 aaaacgtatt tggatttcca caacaactac ttaccaatta cgccaaaagg cacattcttt
    16861 atgcaactga aataaaaata acaagcggtc attttgcaga tttattttgc aaaatgaccg
    16921 cttgtattcg cttttactat ttgcggttca aacagcaatc aatatttaaa catttgcgaa
    16981 gcgggcggat aatcggcgag ccgtttccat catataaaat ctcaatattg acattataga
    17041 gtctctgaat aatatcgcat tccaacatat cacaagctct gccttgtgct tgaatttccc
    17101 ctcgcttaat agcatcacac tatccgaaaa ttgagccgca aggcttaaat cgtgcagcac
    17161 cataattgta attaggttat tcgctcgagt gtaggcattc acatattcaa gtaagttgat
    17221 ttgatgatac atatccaacg cactgacagg ctcatctagc agcagaatgt gtggtttacg
    17281 tagcaatact tgtgcgaaca ttaccatttg ccgttgcccg ccacttagcc gcataatatc
    17341 ttgatgagct agatgtgaaa taccgagttt atccataata tcaacggctt cattgagtaa
    17401 ttcatcgcta atatacatat tcaggctatc aagttgccct aataaaataa cctctaaggc
    17461 actgagtgag gcctctacct gtgtgtcttg tggcatatag ccaatttctt tacgccaact
    17521 atgcaagtgg cttgaggtta atttattacc gtcaaaatca attgtgcctt gatgagcaat
    17581 ttccccaaat agagttttaa ggagagaaga tttacccgca ccatttgggc caagtaaagt
    17641 atagatcttg ccttgttcaa aatgtagatt gattttatca gccaccacta ag
//
LOCUS       DANLXS010000031        17441 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.031, whole genome shotgun sequence.
ACCESSION   DANLXS010000031 DANLXS010000000
VERSION     DANLXS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17441
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.031"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(405..2522)
                     /gene="spoT"
                     /locus_tag="PXD71_000812"
     CDS             complement(405..2522)
                     /gene="spoT"
                     /locus_tag="PXD71_000812"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL6290961.1"
                     /translation="MYLFEPLDRIIQGYLPPEQIERVKRAYVIARDAHEGQTRSSGEP
                     YITHPVAVASIIAEMKLDQEAVIAALLHDVIEDTPYTEEELAAEFGQSVADIVQGVSK
                     LDKLKFRTRQEAEVANFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
                     ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYRVLKMAIASARGHRKELIQRIAD
                     DIRGRLEEVQIEARVYGREKHLYSIYQKMRQKDQHFHSILDIYAFRVVVDNVDNCYRV
                     LGQMHALYKPRPKRVKDYIAVPKPNGYQSLHTSMIGPHGVPVEVQIRTEDMDKMADMG
                     VTAHWRYKEGESHHNTTVQLKAQQWLQSIVELQNSAGNTAEFIDSVKSDLFSDDIFVF
                     TPKGRIVELPANATAIDFAYAVHSGIGDRCVGAMVDRNPYPLSEPLRTGQTVEIITEQ
                     GKRPNASWLNFVVSGKARAKIRQSLKNQQHDEAIALGRRQLVRAMNLRSLDELCSETI
                     QSVLDELRLSSFDELLAEIGLGNQMSNVIAQRLLGEPLLIDTDGNPENNQPLKIEGIE
                     NRLISFANCCLPIPGDEIIAHVSSGKGLAIHNACCGNVKDYATEPSNYLPVEWEIAKE
                     KAIQFETEIVLDIINEPGVLAGITSTLAKLNSNIGSINSEAREGNIYQVRVQISVENK
                     EHSELILRKLLSVKGVVKAARKQ"
     gene            complement(2632..2913)
                     /gene="rpoZ"
                     /locus_tag="PXD71_000813"
     CDS             complement(2632..2913)
                     /gene="rpoZ"
                     /locus_tag="PXD71_000813"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL6290962.1"
                     /translation="MARVTVQEAAEKIGNRFDLILIAARRARQLQLHTREPLVPEEND
                     KPTVIALREIEEGLITTDIMDQLENHDSIKQEAAEQEAISFLTEMSNEA"
     gene            complement(3016..3513)
                     /locus_tag="PXD71_000814"
     CDS             complement(3016..3513)
                     /locus_tag="PXD71_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL6290963.1"
                     /translation="MAHFYEYLEFNSDEDRENQLEVYVEVKLSTDTEEKLQAVGVQGD
                     WLVMAEPYCPDCVEIVAYFQRMAKLNPNIKVKYVARKDNQERKHFDSDEQQQAVISAQ
                     KIPTIFDIRNGKTELVLSEFPQFLKDKMAANPEQFEQLKADFRMGKFGKEVEAELLAI
                     LLNTK"
     gene            complement(3538..4560)
                     /locus_tag="PXD71_000815"
     CDS             complement(3538..4560)
                     /locus_tag="PXD71_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6290964.1"
                     /translation="MSVTKKRLTLSDIAELSGVSKTTVSMILNGRGNDFRIKSETCQK
                     VKEIAKKHGYRANVYAKSLQAQRTNVIGLVIPDLTNYGFALTSRTLEKLCRENGLQLV
                     IACSDDTFEGEKKAIERLLDRQVDLLITAPTHNEPTYYQNAAQYTPMLQLDRYISGLN
                     IPSITSNDTQSISMLVENIVRAYHLKEFFYLGGQLSLSPSQNRLKGFREGLEQSHLAL
                     DENWVLHQDYQPESGYQMFAQIVEKLGRLPEAVFTASFTLLEGVMRYLTEHQQMDKLL
                     TQELHLATFDDHHLLNALPFHIHSIKQDHQQIAERTFELIQQKLAQQKIDDVKVDCEI
                     LWRKSI"
     gene            complement(4600..6030)
                     /locus_tag="PXD71_000816"
     CDS             complement(4600..6030)
                     /locus_tag="PXD71_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL6290965.1"
                     /translation="MHIFNNGKYKSLHAQSEGELNALRQTVLSDPDFYPTYHLAPETG
                     LFNDPNGLIFDGEKYHIFAQWFPYGALHGMKHWQHFITTDFQTFSKGDLLIPDELFES
                     HGCYSGGAILWQDKIVAFYTGNTRRPSDNARIPHQNIAIFDKSGKLLEKRCIIDQAPA
                     GYTEHVRDPKPYLTAEGKIRFVLGAQRENLTGTALIYEMDDLNATPRLIAELEVHNFD
                     NSNVFMWECPDLFSLGGKDLFVWSPQGKLRESHQFQNNYHATYALGELNGNRLRAEHI
                     EELDYGFDFYAPQTVENSDRILFGWVGLPDLTYPTDKYQWHSMLTLPRKLSLQNGKVI
                     QNPIVNVGELQAVEMSENFAIENLDTAYLQISVENQPLVLDFFSNEQGQKLSLRFENG
                     VLSLDRSQTEQTELMEKFGSVRHCKIEQLETIEIFFDRSVVEIFVNGGEKVLTSRFFI
                     AKRENVVKSSRPIQVKVAKVLPISVE"
     gene            complement(6135..7058)
                     /locus_tag="PXD71_000817"
     CDS             complement(6135..7058)
                     /locus_tag="PXD71_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDL6290966.1"
                     /translation="MSKIWVTGDAVVDLIPDGEHHYLKCAGGAPANVAVGVSRLGVEA
                     GFIGRVGNDPLGKFMREVLQAENVCTKQMILDAQHRTSTVIVGLDNGERSFTFMVNPS
                     ADQFLEIGDLPEFNQGDFLHCCSIALINDPSRSTTIEGIRRVKKAGGYVSFDPNLRES
                     LWKSLDEMKTVVNSVVAMADILKFSEEELTLLTETETLEQATQAITAQYPEKLIIITL
                     GKDGAIYHFNGKSQVVAGKALKPVDTTGAGDAFVSGLLAGLAQTPNWHDETVLVEVIR
                     KANASGALATTAKGAMSALPNKAELDAFLAE"
     gene            complement(7175..8602)
                     /gene="miaB"
                     /locus_tag="PXD71_000818"
     CDS             complement(7175..8602)
                     /gene="miaB"
                     /locus_tag="PXD71_000818"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDL6290967.1"
                     /translation="MAKLHITTWGCQMNEYDSSKMADLLNSTHGFELTENPEDADVLL
                     LNTCSIREKAQEKVFSQLGRWKNWKKDKPNLIIGVGGCVASQEGEHIRDRAPYVDIVF
                     GPQTLHRLPEMINQIRGGKSSIVDISFPEIEKFDRLPEPKAEGPTAFVSIMEGCNKYC
                     SFCVVPYTRGEEVSRPVDDVLFEIAQLAAQGVREVNLLGQNVNAYRGETFDGGICTFA
                     ELLRLVAAIDGIDRLRYTTSHPIEFTDDIIEVYRDTPELVSFLHLPIQSGSDRVLTMM
                     KRNHTALEYKAIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFS
                     FIYSARPGTPAADLPDNVSDDEKKERLSRLQQRINHQAMQFSRAMLGTEQRILVEGPS
                     KKDLMELTGRTETNRVVNFVGTPDMIGKFVDVKITDVYTNSLRGEVIRTEDQMGLRVV
                     ESAESVIARTRKEDDLGVGKYVVNL"
     gene            complement(8819..9319)
                     /gene="crr"
                     /locus_tag="PXD71_000819"
     CDS             complement(8819..9319)
                     /gene="crr"
                     /locus_tag="PXD71_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005702996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL6290968.1"
                     /translation="MGLFDKLFGSKQKESQEIKIYAPLTGEIVNIEDVPDVVFSEKIV
                     GDGVAIRPSGDTIVAPVNGTIGKIFETNHAFSIESEEGVELFVHFGIDTVELKGEGFT
                     RLAQEGQTVKVGEPIIKFDLALLEGKAKSVLTPIVISNMDEISNLSKLNGQVVAGESV
                     VLTLTK"
     gene            complement(9404..11125)
                     /gene="ptsI"
                     /locus_tag="PXD71_000820"
     CDS             complement(9404..11125)
                     /gene="ptsI"
                     /locus_tag="PXD71_000820"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDL6290969.1"
                     /translation="MITGIAASPGAVFGKALVLKEEPIVLNTRKITESEVEAEKAKFF
                     TGREKAAAQLTAIKEKARRTLGEEKEAIFEGHLMILEDEELEEEILSYLTDNLVTADV
                     AASKVIEMQASMLAEIDDEYLKERAGDIRDIGNRLVRNILNMNIVDLGDIQDEVILVA
                     YDLTPSETAQLNLDKVLGFITDVGGRTSHTSIMARSLELPAIVGTNNVTSLVKTGDFL
                     ILDAVNNEVHLNPSEEKIANFKALQEKLNAEKEELAKLKELPAQTLDGHKVDVAGNIG
                     TIRDVEGVLRNGGEAVGLYRTEFLFMDRTQLPGEEEQFQAYKEIVEAMNGQMVILRTM
                     DIGGDKELPYLDLPKEMNPFLGWRAVRIGFTRREILDTQLRAVLRASAFGNLAVMFPM
                     IISVEEIRLLKSIVAELKDQLRAEGHAFDENLKLGIMVETPSAAVNARHLAKEADFFS
                     IGTNDLTQYTLAVDRGNEIIAHLYNPLSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     VRATALLLGMGLDEFSMSGISVPHVKKLIRSINFADAKALADEALAQPTAADIERLVD
                     EFYQKLN"
     gene            complement(11247..11504)
                     /gene="ptsH"
                     /locus_tag="PXD71_000821"
     CDS             complement(11247..11504)
                     /gene="ptsH"
                     /locus_tag="PXD71_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDL6290970.1"
                     /translation="MYSKDVVITAPNGLHTRPAAEFVKAAKGFASDITVTSGGKSASA
                     KSLFKLQTLGLTQGTTITISADGEDEQKAVDFLVDLIPTLE"
     gene            11765..13339
                     /gene="thiP"
                     /locus_tag="PXD71_000822"
     CDS             11765..13339
                     /gene="thiP"
                     /locus_tag="PXD71_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease"
                     /protein_id="HDL6290971.1"
                     /translation="MLSTLTKTTAWFIYFSIIALYVFSLWALMDYHQNDTLFVFSDIT
                     ATVAYSLLQASLSAVISLFLGSLLARSFFYLDFKGKNLLYKTISFIWALPSLVIIFAV
                     IGVFGNSGWLAKLFQQLGWNWQFNLYGLHGIILAHCLFNIPFVMKYYTGGLQLTPSSR
                     FQLAAQLNLTGWNYIQIVELPIIKTLLPYMFMTVFLVCFTSFPIVLMLGGSPKYSTLE
                     VAIYQAVTFEFDFAKALILIRVQFVVGIGLQLVMNYISTSALKKLKQQPNRVEIWKPK
                     PTGLAKLGLQAVIFLQSFAIILPLVSVIWAGLSVSKLSERLLNPTLWQATQYSLYLSL
                     IASIFVITISYLIALEARQLLYTKAKLRYSILTAITLFPLLLPIFLLSVGLFILLMNS
                     ELSSEALLLLVGICNGLTLLPFIYPMLFSAMWNSLTSHDKLAQGLGLKGLRRWWIVEK
                     NQLIRPLVTVFALAMSSSLGSFTVIAFFGNADFSSLPYLLYQQLGSYRTEDAAVTALI
                     LMLFALLPFLFSKDKL"
     gene            13336..13986
                     /gene="thiQ"
                     /locus_tag="PXD71_000823"
     CDS             13336..13986
                     /gene="thiQ"
                     /locus_tag="PXD71_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HDL6290972.1"
                     /translation="MIELDLHFTYEQMNMHFQLSVKKGERIAVVGESGAGKSTLLNLI
                     AGFEKAESQQSQLWLNQKNHLDTEVAQRPVSMLFQDNNIFPHLTVEQNIGLALVPSLS
                     LNPKQKAQVKQIATQMGIETFLQRQADQLSGGQKQRVALARTLLRDKPILLLDEPFSA
                     LDPQRRAELQQIVFDICQSRNLTLLMVTHQLSESLFLFNRVITVADGEIVSDLSPL"
     gene            14293..15707
                     /locus_tag="PXD71_000824"
                     /pseudo
     CDS             14293..15707
                     /locus_tag="PXD71_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
     gene            complement(15823..16626)
                     /gene="map"
                     /locus_tag="PXD71_000825"
     CDS             complement(15823..16626)
                     /gene="map"
                     /locus_tag="PXD71_000825"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL6290973.1"
                     /translation="MNNIPLRNEEEIQKLRVACKLASDVLVMIAPYVKEGVTTGELDK
                     ICHEYILANGATSASLGYHGFPKSVCISLNEVVCHGIPSDDKKLKKGDILNIDVTVIK
                     DGYYGDNSMMYVVGEPSVRDKRLMAVTLESLYVGIRTVKAGVRLKEIGTAIQKYVEKE
                     GFSVVREYCGHGIGTEFHCDPQVVHYATDDGGVVLKEGMVFTIEPMVNAGKKEVRLMG
                     DGWTVKTKDRSHSAQYEHQIVVTKDGCEVLTIRDEEIAEGRISRIMHNS"
     gene            complement(16712..17377)
                     /locus_tag="PXD71_000826"
     CDS             complement(16712..17377)
                     /locus_tag="PXD71_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDL6290974.1"
                     /translation="MNFTTYFSDENKRRSLILLSLFHIFIIAISNYLVQISFEVQVPL
                     TEFSFPTTWGTLTFPFIFLATDLTVRVFGADLARKIIFVVMFPALVISYVISVLFFET
                     QFQGFSALTEFNFFVFRIALASFAAYLVGQLLDVLVFNSLRQGKRWWLAPTCSTIFGT
                     LIDTFVFFAIAFYKSSDEYMAEHWFTIGSVDYAFKLFVSLLLFLPLYGVILNFIMKKL
                     DLK"
BASE COUNT         4965 a         3861 c         3266 g         5349 t
ORIGIN      
        1 cattttcgag aaaattttcg ctaagcccac catcttgatt aggctttcta gatatatgcc
       61 aaaatccaga taaacgataa taccctacct gagataattt tttctctgca tagcttttat
      121 catttatgag cattcctcta gatatgagta gttctactaa ttcgtgatac tctttatgtg
      181 gttttgtagg cataaaattg aaggcatttt aagaaaggca aggacgtagc ttgacactct
      241 aagtcgatta catttagtaa acctcaggga caggggctac gccacgattg caagggatta
      301 tacattttag ctgattaaag tcaaaaggtt tcatcaatta ataatagcaa tactgtactt
      361 ttaaggacta taattgcaaa aaactgataa aattttaccg cttgttactg cttacgggcc
      421 gcttttacaa caccttttac acttaatagc ttacggagaa tcagctcaga atgctcttta
      481 ttttccacag aaatttgtac tctcacttga tagatatttc cttctcttgc ctcgctatta
      541 atgctgccaa tgttgctatt taatttggca agcgttgatg tgatccccgc gagtacccct
      601 ggttcattga taatatccag tacgatttcc gtttcaaatt gaatcgcttt ttctttggca
      661 atttcccatt ccacaggtaa ataattactc ggctctgttg cgtaatcttt cacattacca
      721 caacaagcat tgtggattgc caatccttta cctgaactga cgtgagcaat aatttcatca
      781 cccggtatag gtaagcaaca gtttgcaaaa ctgataaggc ggttctcaat cccttcaatt
      841 ttaagcggtt gattattttc tggattacca tcagtatcaa ttaatagtgg ctcccctaat
      901 aagcgttgag cgataacatt actcatttgg ttacctagac caatttcagc cagtagctca
      961 tcaaagcttg ataaacgtag ctcgtctaat acggattgaa tagtttctga acacaattca
     1021 tcaagtgagc gtaaattcat tgcccgaact agctgtctac gcccaagtgc aatcgcctca
     1081 tcgtgctgct gatttttaag cgattgacga attttagccc tcgccttacc acttactaca
     1141 aaatttaacc acgatgcatt tgggcgctta ccttgctcgg taataatttc caccgtctgt
     1201 cctgttcgta gcggttctga aagtggatac ggattgcgat caaccatcgc acctacacaa
     1261 cgatccccaa tgcctgaatg caccgcatag gcaaagtcaa tagcggtagc attcgccggc
     1321 aattcaacaa tacgcccttt cggggtaaag acgaaaatat cgtcagagaa cagatccgat
     1381 ttaacgctat caataaattc tgctgtattg ccggcactgt tttgcaattc aacaatactt
     1441 tgtagccatt gctgggcttt gagttgaacg gtcgtgttat ggtgcgactc gccctctttg
     1501 taacgccagt gagctgttac ccccatatcc gccattttgt ccatatcttc ggtgcgaatc
     1561 tgtacctcca ccggtacacc gtgcgggcca atcatcgaag tgtgtaaaga ttgatagccg
     1621 ttcggttttg gcacggcaat ataatctttc acccgtttcg gacgaggctt atacagtgcg
     1681 tgcatctgcc ctaatacacg gtaacaatta tctacattat caaccactac tcggaaagca
     1741 taaatatcta aaatcgaatg aaaatgttga tctttctggc gcattttttg gtaaatggaa
     1801 tagagatgtt tttcacgccc atacacacgg gcttcaattt gcacctcttc taaccgacca
     1861 cgaatatcat cggcaatgcg ttgaattagc tctttacgat gtcctctggc actggcaata
     1921 gccattttca gtacccgata acgttgcggg tgcattgcct cgaagcctaa atcttccaac
     1981 tcgtttttaa tatgctctat tcccaagcga tgtgccaacg ggctataaat ctctaaggtt
     2041 tctttcgcta tacggcggcg tttatccgga cgcaaagcac caagcgtacg catattatgg
     2101 gttcggtcgg caagtttaat taacacaacc cgaatgtctt tggtcattgc caaaatcatt
     2161 ttacggaagt ttgccacttc tgcctcttgg cgggtgcgga attttaattt atccagctta
     2221 gaaacgcctt gtacaatatc tgctacgctc tgtccaaatt ccgccgccag ttcttcttcg
     2281 gtgtaaggtg tatcttcaat cacatcgtgc aacaaagccg caatcaccgc ttcttgatcg
     2341 agcttcattt ccgcaataat cgacgccact gctaccgggt gagtaatata gggctcaccg
     2401 ctagagcgtg tttgtccctc gtgagcatca cgggctatca cataagcacg ttttacacgc
     2461 tcaatctgct cgggtggcaa atagccttga ataattcgat ctaaaggttc aaaaagatac
     2521 acgttatacc tcaacaacag aaataaaaat ggacaacgaa tttgcaaatt ttgaggaaaa
     2581 tcctaccgct tgcagactca attgtccatt ctctatttac gaaaaataaa attacgcttc
     2641 gttgctcatt tcagttaaaa atgaaatcgc ctcttgctcc gccgcttctt gcttaataga
     2701 gtcgtgattt tccagctgat ccataatgtc agttgtaatt aaaccttctt caatttcacg
     2761 taatgcaatc acggtcggct tatcgttttc ttcaggcaca agtggctcac gggtgtgaag
     2821 ttgtaattgt cttgcacgac gtgcagcaat taagattaaa tcaaaacggt tgccaatttt
     2881 ctccgctgct tcttgtacgg ttacacgagc cataaattta ctccaaagat ttattcggtt
     2941 ttattcataa aaagattgcg aattatacag aaattccgcc aattccgcaa tgaaagcggg
     3001 ctaaattcac aaaatttatt tggtattcag taaaatcgcc aataattcag cctcaacctc
     3061 tttgccgaat tttcccattc taaaatcggc ttttagttgc tcgaattgct cagggttcgc
     3121 tgccatttta tcttttaaaa attgcggaaa ttcagataac actaattctg ttttgccatt
     3181 acgaatatca aaaatagttg gaattttttg cgcagaaatg accgcttgtt gctgttcatc
     3241 gctgtcaaaa tgtttgcgtt cctgattatc tttgcgagcc acatatttca ccttaatgtt
     3301 aggattgagc tttgccattc gctggaaata agccacaatt tccacacaat cggggcaata
     3361 tggctctgcc attaccagcc agtccccttg cacgccaaca gcttgcagtt tttcttccgt
     3421 atctgtggaa agcttgacct ccacatacac ttctaattga ttttcacgat cttcatcgct
     3481 gttaaattct aaatattcgt aaaaatgtgc cataattttg tcttttccta tctcatctta
     3541 aatggattta cgccataaaa tttcacaatc aactttaacg tcatctattt tctgttgcgc
     3601 taacttttgc tgaattaatt caaaggtacg ttcggcaatt tgttggtggt cttgcttaat
     3661 tgaatgaata tgaaaaggta gtgcattcag taaatggtga tcatcaaagg ttgctaaatg
     3721 cagttcttga gtcaataatt tatccatttg ttggtgttca gtcagatagc gcattactcc
     3781 ctcaagtagg gtaaaagagg ctgtgaatac cgcttctggg agcctgccaa gtttttcaac
     3841 tatctgagcg aacatttgat agcccgattc cggctgatag tcttgatgca gaacccaatt
     3901 ttcatcaagt gccaaatgtg actgctccaa accttcacgg aagcctttta aacgattttg
     3961 acttggcgag agtgaaagtt gtccacctaa gtaaaaaaac tcttttaagt gataagcccg
     4021 tacaatattt tccaccaaca tactgatact ttgtgtatca ttactggtga tagaagggat
     4081 attcaaccca gaaatatagc ggtctagctg taacattggg gtatattgtg ccgcattttg
     4141 ataataagtc ggctcattat gtgtcggagc agtaatcagt aaatctacct gtctgtctaa
     4201 taaacgctct atcgctttct tctcaccttc aaaggtatca tccgagcaag cgatcactag
     4261 ctgtaacccg ttttcacggc ataatttctc taaggttctt gaggtcagag caaagccata
     4321 attcgttaaa tcgggaatga ctaaaccgat cacattcgtg cgttgagcct gtaatgattt
     4381 tgcatataca ttagcacgat aaccgtgttt tttagcgatc tctttcactt tttggcaggt
     4441 ttcggatttg attctaaaat cattgccccg cccatttaaa atcatactga ctgttgtttt
     4501 agatacgccg ctgagttccg caatatcact gagcgttagg cgttttttag tcacacttat
     4561 ctccttttag cataaaaatg ggcattactg cccattgctt tattctacgc taattggcaa
     4621 tacttttgct actttcactt gaatcggacg agagcttttt acgacatttt cacgcttagc
     4681 gataaaaaag cgtgaagtca gcactttttc cccgccgttt acgaaaattt ccaccacaga
     4741 acggtcaaag aaaatctcga ttgtctctaa ttgttcaatt ttgcaatgac gaaccgagcc
     4801 aaatttttcc atcaattctg tttgttcggt ttggctacga tctaagctca acaccccatt
     4861 ttcaaaacgt aagctgagct tttgcccctg ttcattgctg aaaaaatcca acacaagcgg
     4921 ttgattttcc acagaaattt gcaagtaggc agtatccaaa ttctcgattg caaaattttc
     4981 tgacatttcg accgcttgta attcgcctac attcacgatt gggttttgta tcactttgcc
     5041 gttttgcagg gaaagtttac gaggcaatgt cagcattgaa tgccactgat atttatcagt
     5101 tgggtaagtg agatcaggca agccgaccca gccaaacaaa atgcgatcgg aattttccac
     5161 cgtttgcggt gcgtaaaagt caaagccgta gtccagctct tcaatatgct ctgcccgtaa
     5221 tctgttgccg tttagctcgc caagtgcgta agtggcgtgg tagttatttt ggaattgatg
     5281 gctttcacgc agtttgcctt gtggcgacca cacaaacaga tctttaccac caagtgagaa
     5341 cagatccgga cactcccaca taaacacatt gctgttatca aaattgtgga cttccagttc
     5401 tgcgattaaa cggggtgtgg catttaaatc gtccatttca taaattaacg ccgtgccggt
     5461 caagttttca cgctgagccc ccaacacaaa acgaattttg ccctctgcgg taaggtaagg
     5521 tttcgggtca cgcacgtgtt cggtgtagcc tgccggtgct tggtcgataa tgcaacgctt
     5581 ttccaataat ttgcccgatt tatcaaaaat cgcgatattt tggtgaggaa tgcgggcatt
     5641 atctgatggg cggcgtgtat tgccggtgta aaaagcgaca atcttatctt gccataaaat
     5701 cgccccacct gaataacagc cgtgggattc aaacagctca tcgggaatca gcaaatcccc
     5761 tttactgaac gtttgaaaat cggtagtgat gaaatgctgc caatgtttca ttccatgtaa
     5821 tgccccataa ggaaaccatt gggcgaaaat atggtatttt tcaccatcga aaatcaagcc
     5881 gtttgggtca ttaaataagc ctgtttcggg agcgagatga taggttgggt aaaaatcggg
     5941 atcagataaa acggtttggc gaagagcgtt taattcgccc tcgctttgtg cgtgcaggct
     6001 tttgtatttg ccgttgttga aaatgtgcat agagtgttcc tgtcatttca gaaattacga
     6061 tggtgcaagc gtccacgctt gtaccctttt gtaaaaaatg aggcacaagc gtggacactt
     6121 gcgccatcat tacattattc cgccaaaaat gcgtctaact ccgctttatt cggtaatgcc
     6181 gacatcgccc cttttgctgt ggtggctaac gcaccggaag catttgcttt gcggatcact
     6241 tcaactaaca cagtttcatc gtgccaattc ggcgtttgag caagaccggc taataatccg
     6301 ctgacaaagg catctccggc accggtggta tccacaggtt tcaaggcttt gccggcaacg
     6361 acttggcttt ttccgttgaa atggtagatt gcaccgtctt tgccaagggt gataataatc
     6421 aatttctccg gatactgagc ggtaatagct tgtgtggctt gttccaaagt ttctgtctcg
     6481 gttaataagg tcagctcttc ttcggaaaat ttgagaatat ccgccatcgc caccacagaa
     6541 tttaccactg ttttcatttc atcaaggctc ttccagagcg attcacgcaa gtttggatca
     6601 aacgaaacat agccacccgc ctttttcact cggcgaatcc cttcaatggt agtggaacgg
     6661 gatgggtcgt taatcaaggc aatagaacag cagtgtaaga aatcaccttg gttaaattca
     6721 ggtaaatcgc cgatttctaa aaattggtcg gcactcggat ttaccataaa agtaaaacta
     6781 cgctcaccgt tatctaagcc aacaatcacg gtggaagtgc ggtgctgagc gtctaaaatc
     6841 atctgtttgg tgcaaacatt ttctgcctgt aacacttctc gcataaattt acccagaggg
     6901 tcgttaccca cacgcccgat aaatcctgcc tcaaccccta aacgagaaac gcctactgca
     6961 acattcgccg gagcaccacc ggcacatttt aaatagtgat gttcgccgtc gggaattaaa
     7021 tccaccaccg catcgcctgt tacccaaatc ttactcatct catacacctt taattagtca
     7081 caaaacacca agctaaaacg atttagcatt tattcgatta tatactaaaa gaccgcttgt
     7141 acatacctac aagcggtcat tttttcttta atttttacag attaaccacg tatttaccta
     7201 cacctaaatc atcttcttta cgagtgcggg caatcacgct ttctgccgat tccaccacac
     7261 gtaatcccat ttggtcttcg gtacgaatca cttcgccacg gagtgagttg gtataaacat
     7321 cggtgatttt cacatcgaca aatttgccaa tcatatccgg tgtgccaaca aagtttacta
     7381 cacggttagt ttcggttctg ccggttaatt ccatcaagtc tttcttagac gggccttcaa
     7441 ccaaaatgcg ttgttctgta ccgagcattg cacggctaaa ttgcattgct tggtggttga
     7501 tgcgttgttg taaacgggat aatcgctctt ttttctcatc atcggagaca ttatcaggca
     7561 gatctgccgc tggtgtaccc ggtcgagccg agtagataaa gctaaagctc atatcaaagt
     7621 tcacttgctc gatcactttc attgtttgct cgaaatcctc tgcggtttcg cccgggaagc
     7681 ctacaataaa gtccgaacta atttgaatct caggtcgaac agcacgcaat ttacggataa
     7741 tcgctttgta ttccaacgct gtgtggttac gcttcatcat tgttaataca cggtctgaac
     7801 cactttgaat cggtaaatgt aagaaactga ccaattcagg tgtatcacga taaacttcga
     7861 taatatcatc ggtaaattca atcgggtggc tggtggtgta acgtaagcgg tcaataccgt
     7921 caatcgctgc cactaagcgt aataattcgg caaaggtaca aatgccgcca tcaaaagttt
     7981 caccacggta agcatttacg ttttgaccga gtaaattaac ctcacgcaca ccttgtgccg
     8041 caagttgggc gatttcaaat aatacgtcat ccaccgggcg ggaaacctct tcgccacgag
     8101 tgtaaggcac aacgcagaac gaacaatatt tattgcagcc ttccataatt gaaacgaatg
     8161 ctgtagggcc ttcggctttt ggctccggca agcggtcaaa tttttcaatt tccgggaaag
     8221 aaatatcgac aatcgagctt ttaccgccac ggatttggtt gatcatttca ggcaaacggt
     8281 gcaaagtttg tggcccgaaa acaatatcta cataaggggc acgatcacgg atatgctccc
     8341 cttcttgtga agccacacaa ccgccaacac cgataatcag gttcggttta tcttttttcc
     8401 agtttttcca acgcccgagt tgcgagaata ctttttcttg tgctttttca cggatagaac
     8461 aagtgtttag cagcaacacg tctgcatctt cagggttttc agtcagttca aaaccgtgag
     8521 tagagtttag gagatccgcc atttttgatg agtcgtactc attcatttgg cagccccaag
     8581 tggtaatatg taatttagcc ataacgttta aaattaataa ataattcaat aacttagaaa
     8641 agaaaagcgt aaagcaattt attatttttg acttaaaata aaccctttac gcaaaatttg
     8701 tggcgttatt ttaccttgtt gcctattttt tatctataaa taatcgccat ttatccgatt
     8761 ttacctaaaa caaaaaagcc acgatcttcc atcgtggctt ccagctaagt aggaggtatt
     8821 atttggttaa ggttaaaacc acgctttcac cggcaacaac ttggccattt aatttagata
     8881 agttgctgat ttcatccata tttgaaatca cgattggtgt taatactgat ttggctttgc
     8941 cttcaagtag agctaaatca aatttaatga tcggctcgcc cacttttacg gtttgacctt
     9001 cctgtgcaag gcgagtaaag ccttcgcctt ttaattcaac agtatcaata ccgaagtgaa
     9061 caaataattc cacgccttct tcagattcaa tcgaaaaagc gtggttggtt tcaaaaattt
     9121 tgccgatagt cccatttact ggagcaacga tagtgtctcc actcgggcga attgccacac
     9181 cgtcacctac aattttttca gagaaaacaa catcaggcac atcttcaata tttacgattt
     9241 cacctgttaa tggcgcatag attttgattt cttgactctc tttttgtttt gagccgaata
     9301 atttatcaaa taaacccatc ttaaaatctc ccttagatat tagatacaaa attctcgtac
     9361 attttatacg agaattttgt atttgtatgc taaataatta aaattagttt aatttctgat
     9421 aaaactcatc tactaagcgt tcaatatctg cagcagttgg ctgagcaagc gcctcatcag
     9481 ctaatgcttt agcatcggca aaattaattg agcgaatgag ttttttaacg tgtggcacag
     9541 agataccgct catactaaat tcatctaatc ccataccgag caacaacgca gttgcacgca
     9601 cgtcaccggc taattcacca cacatacctg tccatttgcc ttcagcgtgt gaagcatcaa
     9661 taacttgctt gattaagttt aagactgatg ggcttaatgg gttatataaa tgagcaataa
     9721 tttcattacc acgatctacc gcaagtgtat attgggttaa gtcattagta ccaatactaa
     9781 agaagtcggc ttcttttgca agatgacgtg catttaccgc tgctgatggg gtttccacca
     9841 taatacctag ttttaagttc tcatcaaaag cgtgaccttc tgcacgtaat tgatctttca
     9901 attccgcaac gatggatttt aataaacgga tttcttccac cgaaataatc atcgggaaca
     9961 ttaccgctaa attaccgaac gcagaagcac gtaataccgc acgtaactga gtatctaaaa
    10021 tttcacggcg ggtgaaaccg atacgcaccg cgcgccaacc taagaatggg ttcatttctt
    10081 tcggtaaatc aaggtatggt aactctttat cacctccgat atccatcgta cgcagaatga
    10141 ccatttgacc attcattgct tcaacaatct ctttgtaggc ttgaaattgc tcttcttctc
    10201 ccggtaattg agtacgatcc ataaacaaaa actcagtacg gtataaacca accgcttcac
    10261 caccgttgcg taatacacct tccacatcac ggatagtacc gatattccct gccacatcaa
    10321 ctttatggcc atctaatgtt tgagcaggca actcttttaa tttggctaat tcttcttttt
    10381 ccgcatttaa tttctcttgt aatgctttga aattagcgat tttttcttcc gatggattta
    10441 aatgtacttc attgttcact gcatctaaaa tcagaaaatc accagtttta actagggaag
    10501 taacattgtt agtaccaaca atggcaggaa gctctaggga acgagccata atagaagtgt
    10561 gagaagtacg accaccgaca tcggtaataa agcctaatac tttatctaaa tttaattgag
    10621 ctgtttcgga aggggttaag tcataagcga ctaaaataac ttcatcttga atatcaccaa
    10681 gatctacgat attcatattt aaaatattgc gtaccaagcg gttaccaata tcacgaatat
    10741 cacctgcacg ttcttttaaa tattcatcgt caatttccgc aagcattgaa gcctgcatct
    10801 caatcacttt acttgctgca acatccgcag tcacaagatt atcagtaagg taactcaaaa
    10861 tttcttcttc aagctcttca tcttctaaga tcatcaagtg gccttcgaaa atcgcctctt
    10921 tttcctcacc taatgtacgg cgagcttttt ctttaatcgc ggttaattga gcagctgctt
    10981 tttcgcggcc tgtaaagaat tttgcttttt ctgcctcaac ttcgctttca gtaattttgc
    11041 gagtatttag tacaatcggc tcttctttta aaaccaatgc ttttccaaat actgcaccag
    11101 gggaagctgc gatacctgta atcataattg tgtccttaat ttaagatgat gtaaaaatca
    11161 tctggatgtt acatagcaaa acactttatt gtagcttatt aagctacaat aaagtgacat
    11221 aaatcttaaa tccgagaata gaaaaattat tctaaagtag gaatcaaatc aactaagaaa
    11281 tcaactgctt tttgctcatc ttcgccgtcc gcagaaattg taattgttgt gccttgagtc
    11341 aaacctaatg tttgtaattt gaataagctt tttgcacttg cacttttacc acctgaagtc
    11401 acagtgatat cagaagcaaa acctttagct gcttttacaa attcagccgc tgggcgagtg
    11461 tgtaaaccat ttggagcggt aataacaaca tcttttgagt acataaaatc ttcctcttaa
    11521 atttgaaagt taaaaaaatt tgttttaatc catttatttt gcgcacgcaa ctttactaca
    11581 aattcaaatt aaaaacacgc taaaaagcgc aaaaactcgt cgtatcaccc tgctaaatag
    11641 ggtgtttttt aagctaactt aaaaatcatt tgtcgaatat caagattcta gcacacactt
    11701 aagcggaaat gttacaataa tttcgctacc acttctttca cttatattct aaatttggag
    11761 caatatgctt agcacattaa ccaaaacaac cgcatggttt atctacttta gtattattgc
    11821 tctctatgtt tttagtctat gggcgttaat ggactatcac caaaatgata ccttatttgt
    11881 tttttccgat ataacggcca cggtcgctta tagcctactt caagccagcc tctctgcggt
    11941 aatttcacta tttttaggca gcctactggc aagatctttc ttttatttgg actttaaagg
    12001 aaaaaattta ctctataaaa ccatttcgtt tatctgggca ttaccctcac tagtaattat
    12061 ttttgcggta attggcgtat ttggtaactc aggctggcta gccaaacttt tccaacaact
    12121 cggttggaat tggcaattta atttatacgg cttgcacggc atcattttgg ctcactgcct
    12181 gtttaacatt ccgtttgtga tgaaatatta taccggaggg cttcaattga ctccaagcag
    12241 tcgctttcag cttgcggctc aattaaacct aacaggttgg aattatatcc aaattgtgga
    12301 actaccgata atcaaaactt tgctgcctta tatgtttatg actgtttttc tggtctgctt
    12361 taccagtttt ccaatcgtat taatgttagg cggtagccca aaatacagca cgcttgaagt
    12421 tgccatttat caagcggtca cttttgaatt tgattttgca aaagccctca tcttaattag
    12481 ggtacaattt gtagtgggca ttggcttaca attagtaatg aattatatct cgacttctgc
    12541 actcaaaaag ctaaaacagc aacctaaccg agtagaaatt tggaaaccaa agcctaccgg
    12601 actggctaaa ctcggcttac aagcggttat ttttttgcaa agttttgcaa taattctacc
    12661 gcttgtcagc gtcatttggg caggactaag cgtgtcaaaa ctcagtgagc gtttgctcaa
    12721 ccctacttta tggcaagcta ctcagtattc tttatactta agtttaattg cttctatatt
    12781 cgtaatcaca atcagctatt tgattgcact tgaagctcgc caattactct atacaaaagc
    12841 caagctcaga tatagcattc tcaccgcaat caccctattc cccttacttc tgccgatctt
    12901 tttattatcg gttggtttgt ttattttatt aatgaatagt gaactctcaa gcgaggcatt
    12961 actgctactg gtcgggatat gcaacggctt aacattactg ccatttattt acccaatgct
    13021 tttctccgca atgtggaata gcctcacctc gcacgacaaa ttagcacaag gacttgggct
    13081 aaaggggctt cgccgttggt ggattgtgga aaaaaatcaa ctaattcgac cgcttgttac
    13141 cgtatttgcg ttagcaatgt catcgagctt aggcagtttc accgtcattg cttttttcgg
    13201 caacgctgat ttcagctcac tcccttattt actctaccaa caacttggta gctatagaac
    13261 cgaagatgct gccgttaccg cactcattct gatgttattt gcgttactac cattcttatt
    13321 cagcaaggat aaactatgat cgaactggat ctccatttca cttacgaaca aatgaatatg
    13381 cactttcagc tttctgtcaa aaaaggcgaa cgtattgcag tggttggcga aagtggcgca
    13441 ggcaaaagca cattacttaa tctaattgcc ggttttgaaa aggcagaaag tcagcaaagc
    13501 cagctgtggc taaatcagaa aaatcatctt gataccgaag ttgcacaacg tcctgtttca
    13561 atgctattcc aagataacaa tattttcccg cacttaacgg tagaacaaaa catcggctta
    13621 gctttagtac caagtttgtc gctcaatccg aaacaaaaag cacaggttaa acaaattgcc
    13681 acacaaatgg ggattgaaac cttccttcag cgtcaagccg atcagctatc gggcggacaa
    13741 aaacagcgag tggcattagc tcgtactttg ttgcgggata agccgatttt attattggac
    13801 gaacccttct ccgccctcga cccgcaacgc cgagccgaat tacagcaaat cgtgtttgac
    13861 atttgccaaa gcagaaatct aaccctgtta atggtaactc accaactatc cgaaagccta
    13921 tttctattta atcgggtgat aacggtagca gatggggaga ttgtttctga tctctcccct
    13981 ctttagcaaa gcagatctgc taggttctaa atgtagggac gccacgctgg cgtacgcaac
    14041 gatgtcaatt ccaacaatct aacggtgaat gatgttccgc acggatacta gcgttattca
    14101 tttttttcag aactcgcccc ctactttagc aaagagggga actgtaacta atattacctc
    14161 cctattccgc tattctcttc cccaaaatgt gatctacttc acaaatttgg aaatatttta
    14221 aatcttctct tttttagcta aaccgattta gttaaaatgc aaccgattat tttttatgaa
    14281 aggtaaattc aaatgaacta tccaaaaatt gcagaacaaa cgattgcaaa attaggtggc
    14341 aaagaaaaca ttaccacact tgcccactgt gcaacccgtt tacgcttaac cattgctgat
    14401 gaatccaaaa ttgataaaga ggcgattgaa aatattgaag gcgtgaaagg tcaattctct
    14461 actcccggtc aataccaaat tatttttgga tcaggcacgg taaatgaagt gtatgcccaa
    14521 atgcaaaaac aaatgggtat ggcagatatg tccacctctg aagtagcggc tgccggtgca
    14581 gcaaatcagc cattactaca acgtttggta aaaggcttag ccgatatttt cgtgccgatc
    14641 attccggcta ttgtggctgg cggtttgtta atgggtattc actcaatgct aacggcagtc
    14701 ggtttcttct gggaaggtga atctgttgtt agccgttatc cgaatatttc cgatttagtt
    14761 gatttcatca acactattgc taacgcacca tttgtattct tacctgtatt actcggtttc
    14821 tcagctacac gcaaatttgg cggtaatcca ttcttaggtg cggcacttgg tatgttattg
    14881 gttcacccgg cacttgcaga cggttggaac tatgccaaaa ccttaatgga aggtaacatc
    14941 aaatactgga atattttcgg cttagaaatt gaaaaagtgg gttatcaagg cacggttatt
    15001 ccaactatcg tttcggcttg ggtgttagca acccttgaga aaactttccg taaatttgtg
    15061 ccaagctatt tagataactt ggtgacgcca atgatgtcat tatttatcgc cggtatttta
    15121 gctttcactg taatcggacc attgggtcgt gaagcaggtt cagcgatttc agcaggctta
    15181 acgtggttat atgacagctt aggctttatc ggtggagcaa ttttcggtac attctacgct
    15241 ccaatcgtga ttaccggtat gcaccaaacc tttattgcgg ttgaaaccca attattagct
    15301 gaagtagcac aaaccggcgg tacatttatc ttcccgattg cagcaatgtc taacattgca
    15361 caaggtgcag cctgtttagg tgtagctttt gcaatgaaag atgccaaagt gcgtggttta
    15421 gccgtgccgt caggtatttc agccctgtta ggtatcaacc ggcaatgttc ggagtaaatt
    15481 tacgctaccg ccaagccttt attgcggcga tgatcggttc aggtttagcc agtgcattta
    15541 tcgctttctt taatgtgaaa gcaattgcct taggtgcagc cggtttctta ggtattccgt
    15601 cgattaaacc tgacagcctt gcaatgtatt ctgtcggtat ggtaatttca tttgtagttg
    15661 catttaccat ttcagccatt tgggtgaaaa aagcacaagc gaaataatca aaaatagatc
    15721 acaaaaaagg gattaagtgt tagcttaatc ccttttgttt ttaaacgcct tagcttaaca
    15781 atgagtttcg caagcggtca tttttcgcta aaaatttgca aattagctgt tgtgcataat
    15841 tcgagaaata cgcccttcgg caatctcttc atcacgaatt gtcagcactt cacagccgtc
    15901 tttggtaact acaatttgat gctcatattg tgcggagtgg ctgcggtctt tggttttcac
    15961 tgtccaaccg tcacccatta agcggacttc ttttttgccg gcattcacca ttggctcaat
    16021 cgtaaacacc attccttctt ttaacactac accgccatca tcggtagcat aatgcactac
    16081 ttgtgggtcg caatggaatt cagtaccaat accgtgtccg caatattctc gtactaccga
    16141 aaagccttct ttttccacat atttttgaat cgctgtacca atttctttta aacgcacccc
    16201 cgctttcacg gtgcgaatcc cgacataaag ggactctagc gtaaccgcca ttaagcgttt
    16261 atcccgcaca cttggctcac cgactacata catcatcgag ttatcgccat agtagccatc
    16321 tttgataacc gtcacatcga tgtttaaaat atcgcctttt ttcagctttt tgtcatcact
    16381 tggaataccg tgacaaacga cctcatttaa cgaaatgcac accgattttg ggaagccgtg
    16441 atagcccaaa ctggctgaag ttgccccatt tgccaaaatg tattcgtgac aaattttatc
    16501 taattcacca gttgttacgc cctctttcac ataaggggcg atcattacta aaacatctga
    16561 agccagttta caggctactc gtagtttttg aatttcttct tcgtttctta atggaatatt
    16621 attcattaca ttgctctctg ctatatctgc cataaaaagt aaggggatta tacctttttt
    16681 cagcacaatc cccaaacctc agtttagatt tctactttaa atcaagtttc ttcataatga
    16741 aatttaatat cacaccataa agcggtagaa atagcagtag gctcacaaag agtttaaacg
    16801 cataatcgac cgaaccaata gtaaaccaat gctctgccat atattcatcg ctacttttat
    16861 aaaaggcgat agcaaagaat acaaaagtat cgattaaagt accaaaaatg gttgaacagg
    16921 ttggagcaag ccaccaacgc ttaccttgtc ttaaactatt aaatactaga acgtctaata
    16981 attgcccaac taaataagct gcaaagctag ccagtgcaat acggaaaaca aaaaagttga
    17041 actcagttaa tgcactaaag ccttgaaatt gggtttcaaa gaataaaacg gaaatcacat
    17101 agcttatgac aagtgccgga aacatcacaa caaaaataat ttttctagca agatctgcac
    17161 caaatacacg cacggttaaa tcggtcgcta agaaaataaa tgggaatgtt aacgttcccc
    17221 aagttgtcgg aaagctaaat tctgttagtg gtacttgaac ttcaaagcta atttgcacta
    17281 aatagttgct aattgcgatg ataaaaatat ggaaaagtga aagtaaaatt agagaacgac
    17341 gtttgttctc atccgaaaaa taagtagtaa agttcatatt gtatcctttt tcgttaaatg
    17401 aaaacttgag gtaagggaac tcaagggggc attattctat a
//
LOCUS       DANLXS010000032        17114 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.032, whole genome shotgun sequence.
ACCESSION   DANLXS010000032 DANLXS010000000
VERSION     DANLXS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17114
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.032"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..207
                     /locus_tag="PXD71_000827"
     CDS             <1..207
                     /locus_tag="PXD71_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6290975.1"
                     /translation="KEINSNLGTLTLTTQEDKTGIFPPTIKLINFSSNIEDDIKEIEQ
                     SCLNSRKQLWEKRRKMQEEQKIPN"
     gene            324..3188
                     /locus_tag="PXD71_000828"
     CDS             324..3188
                     /locus_tag="PXD71_000828"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL6290976.1"
                     /translation="MTKQFTMEDRFNPSAVEQALYQHWESQGYFKPSEDINAPSYCIA
                     IPPPNVTGSLHMGHAFQQTLMDTLIRFNRMEGNNTLWQTGTDHAGIATQMVVERKIAA
                     EEGKTRHDYGREAFINKIWDWKAYSGGTISQQMRRLGNSIDWDRERFTMDEGLSNAVK
                     EVFVRLHEEGLIYRGKRLVNWDPKLHTAISDLEVENKESKGSLWHFRYPLANGAKTAD
                     GLDYLVVATTRPETMLGDTAVAVHPEDERYQALIGKSVILPLANREIPIIADEYVDRE
                     FGTGVVKITPAHDFNDYEVGKRHGLPMVNVMTLNADIRAEAEIIGTDGKPLSDYTAPI
                     PADYQGLERFAARKKIVADFEALGLLDQIKPHDLKVPYGDRGGVPIEPMLTDQWYVSV
                     KPLAEVATKAVEDGEIQFVPKQYENLYFSWMRDIQDWCISRQLWWGHRIPAWYDEQGN
                     VYVARDEAEVRAKHNLPADLPLKQDEDVLDTWFSSGLWTFSTLGWPEQTKELKMFHPT
                     DVLITGFDIIFFWVARMIMFTMHFIKDENGKPQVPFKTVYVTGLIRDEQGQKMSKSKG
                     NVLDPIDMIDGISLDDLLEKRTGNMMQPQLAEKIAKATRKEFAEGIVAHGTDALRFTL
                     TALASNGRDINWDMKRLEGYRNFCNKLWNASRYVLTNDKLDLSEGDVEFSLADRWIES
                     QFNRTVETFRTALSQYRFDLVANAIYEFTWDQFCDWYLELTKPIFFKGTDVQRRGASR
                     TLVNVLEKLLRLIHPVMPFITEEIWQKVKGFVGIEADTIMLQKFPQFDPLAIDETAES
                     QINFIKEVIVAVRNIRAESNIAPSKGLDLIARNFSADEVSILNANEVLLKSMAKLDSV
                     KVLENGENAPLSVAKLVANGEILIPMAGFINKEAELARLTKEMDKLKGEVARIEGKLS
                     NEAFVAKAPEQVIAKEREKMQEYLSGLEKLQVQYQEIEAL"
     gene            complement(3259..3852)
                     /locus_tag="PXD71_000829"
     CDS             complement(3259..3852)
                     /locus_tag="PXD71_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL6290977.1"
                     /translation="MLTDQDIFNRYQTKKSEFEKQADISLIGHSLFDMWGDMENGTPN
                     LAGKTVANLGLSGTSTRQYLDVITRQERIQHVGDNVFIFLGVNDIVKEPDYSPKQVLE
                     WIGEIVDSLRKINPNANFFLLEATPVNNITTTNNAAIKELNAYFEANIPSDLTYVKTW
                     EYFADKEGNLDLTLCHDGLHFTQAGYDRLEAILTKHL"
     gene            complement(3987..5441)
                     /gene="gnd"
                     /locus_tag="PXD71_000830"
     CDS             complement(3987..5441)
                     /gene="gnd"
                     /locus_tag="PXD71_000830"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HDL6290978.1"
                     /translation="MSQKGDIGVIGLAVMGQNLILNMNDNGFKVVAYNRTTSKVDEFL
                     EGAAKGTNIIGAYSLEDLAAKLEKPRKVMLMVRAGEVVDQFIEALLPHLEEGDIIIDG
                     GNSNYPDTNRRVKALAEKGIRFIGSGVSGGEEGARHGPSIMPGGDESAWQYVKPIFQA
                     ISAKTDKGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFLKDGLGLSYDEMH
                     EIFKEWKNTELDSYLVDITTDILAYKDEDGEPLVEKILDTAGQKGTGKWTGINALDFG
                     IPLTLITESVFARCVSAFKDQRVAASRLFNKEIGKVEGDKKVWVEAVRRALLASKIIS
                     YAQGFMLIREASEQFGWDINYGNTALLWREGCIIRSRFLGNIRDAYEANPYLVFLGSD
                     PYFKDILENCLADWRKVVAKSVEIGLPVPCMASAITFLDGYTSARLPANLLQAQRDYF
                     GAHTYERTDKARGEFFHTNWTGRGGNTASTTYDV"
     gene            complement(5524..6213)
                     /locus_tag="PXD71_000831"
     CDS             complement(5524..6213)
                     /locus_tag="PXD71_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDL6290979.1"
                     /translation="MKTLGILGGMSAESTVSYYLDINRSVNKVLGGNSSAKILISSVN
                     FEEIVQCQKQGNWQAAAEILSEQARLLEQAGAEGILLATNTMHKIADQISSHISVPFL
                     HILDCVAEAISAQGLQKVALLGTKFTMSDNFYKDGLLERGITSIVPDEETQQEIHRII
                     FEELCLGKITESAKAFYLQTIQQLSEQGAEGVILGCTEIGLLINQNDNPLPFFDTAAL
                     HSQMAVEFILG"
     gene            complement(6223..7110)
                     /locus_tag="PXD71_000832"
     CDS             complement(6223..7110)
                     /locus_tag="PXD71_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDL6290980.1"
                     /translation="MNPIQLIEPNINSKIFTIRGEQVMLDVHLAELYQVDTRTFNQAV
                     KRNEERFPDIFRFQLTENEWLSLRSQFVILNENSGRGQHRKYLPYVFTEQGIAMLSAV
                     LRSEVAVQVSIKIMQAFVQMRHLLQTNSGLVQRLESVERKQLEMSGQLEQVFQSLEGN
                     KPKMQGVFFEGETFNAYQFVTELVRGAKSSLILIDNYVDDTVLTLFTKRSHNVSVTIY
                     TKQISKQLALDLEKYNSQYAHINVKIFDKSHERFLIIDEKELYHIGASLKDLGKKWFA
                     FSKMDLSMSRILEKLAENQ"
     gene            complement(7286..7984)
                     /gene="pgl"
                     /locus_tag="PXD71_000833"
     CDS             complement(7286..7984)
                     /gene="pgl"
                     /locus_tag="PXD71_000833"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDL6290981.1"
                     /translation="MNKVIFPTAQAAVEQIAKEFIEYSHQNRPVHISLSGGSTPKLLF
                     KTLAQAPFNTEIQWQNLHFWWGDDRMVAPTDPESNYGEVQKLLFDHIDIPSENIHRIR
                     GEENVEQELARFSAELTACVPNLAFDWIILGMGTDGHTASLFPHQTDFNDQNVAVIAK
                     YPETGQIRISKTASLIEKAKRITYLVTGASKAEILKEIQTTPAENLPYPAAKIQAENG
                     VTEWYLDSEAANLL"
     gene            complement(8124..9611)
                     /locus_tag="PXD71_000834"
     CDS             complement(8124..9611)
                     /locus_tag="PXD71_000834"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL6290982.1"
                     /translation="MNAENSCIVIFGASGDLTFRKLIPALYNLYKIGRLGEHFSVLGV
                     SRSELTDESFRQKMRDALVKFEKASGEELDKFCEHLYYQAVNTSDAVDYAKLLPRLDE
                     LHDKYQTAGNTLYYLSTPPSLYGVIPECLAAHGLTTEEFGWKRIIVEKPFGYDIETAK
                     KLDVQIHKCFEEHQIYRIDHYLGKETVQNLLVLRFSNGLFEPLWNRNFIDYVEITGAE
                     SIGVEDRGGYYDGSGAMRDMFQNHLLQVLAMVAMEPPAIINANSMRDEVAKVLYCLHP
                     LTEEDVKHNVVLGQYARGTVDGKEVPAYLEEKGVPADSNTETFMAVKCKIDNWRWAGV
                     PFYVRTGKRLPTRVTEIVIHFKTTPHPVFSQNAPENKLIIRVQPDEGISMRFGLKKPG
                     AGFEAKEVSMDFRYSDLSSSSSLLTAYERLLLDALKGDATLFARTDAVHACWKFVQPI
                     LAYKANRGRVYEYESGTWGPTEADKLIARHGKVWRKPSGTMKKKV"
     gene            complement(9686..10504)
                     /gene="cysQ"
                     /locus_tag="PXD71_000835"
     CDS             complement(9686..10504)
                     /gene="cysQ"
                     /locus_tag="PXD71_000835"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDL6290983.1"
                     /translation="MQTLTQPLLENIINIAKAAGEQLKQLYARSVAIKIKADCTPVTE
                     ADLIISQFLTERLKQLTPDIPVLSEEFCDIPFAERQQWQEYWIIDPIDGTQQFINRTG
                     QFSVVIALVQKREGRNQPVLGVIHAPILGKTYFSMEGSGSFLQENGEIRPLVKPKRAE
                     NALLIVTVGSDLHAEQAKNAISAPQIECVKYGSGSLKAGLVAEGKADCYIRFGDTGEW
                     DTAVAEVLLSEMGGKIFDFNFSPLTYNQRESLINPYFVMVADGSIDWDKIFLFK"
     gene            complement(10518..11060)
                     /gene="nudE"
                     /locus_tag="PXD71_000836"
     CDS             complement(10518..11060)
                     /gene="nudE"
                     /locus_tag="PXD71_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDL6290984.1"
                     /translation="MIKQLPEILNIKTVAKSRIFEVQAVDLRFSNGEERTYERLTPNR
                     RSSVMVIPIQNNELIFVKEYAVGSERYELGFPKGIVDAGEEPIESANRELQEEIGFGA
                     KKLDFLRSLYSSPSHMYGLMHLFLAQDLYPSKLEGDEPEPLEIVRYPLDKIDQLLADP
                     DFAEARNLSALFMLREYLKK"
     gene            11148..11564
                     /gene="hslR"
                     /locus_tag="PXD71_000837"
     CDS             11148..11564
                     /gene="hslR"
                     /locus_tag="PXD71_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDL6290985.1"
                     /translation="MKNKHEKEQNDAEVRLDKWLWAARFYKTRSIAKAMIEGGKVHYN
                     GQRAKVSKAVEIGAIIKLRQGNEEKEVEVLALSDQRRGAPEAQLLYSETEKSIKHREA
                     MAFARKANALSMPHPDRRPNKKERRDLIKFKEQEFS"
     gene            11808..12701
                     /gene="hslO"
                     /locus_tag="PXD71_000838"
     CDS             11808..12701
                     /gene="hslO"
                     /locus_tag="PXD71_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL6290986.1"
                     /translation="MNYTKDNDKLYRYLFKDRAVRGEWVRLNNTFTETLNTHHYPKAV
                     QNLLGEMLVATSLLTATMKFNGNITVQIQGDSPLKLAVVNGNDQQQLRALARVQADIA
                     DTASLAEMIGNGVLVISIIPTDGERYQGVIALDKPTIRECLEDYFARSEQLQTHLVIR
                     TGEFEGKAVAGGMLLQIMPDGTGSRDDFEHLATLTETVKDEELFGLEAEELLYRLYHE
                     EQVEVYPPQDTEFKCGCSRERSGSALLLVPAEEIEEMLEEKQGVIDMQCECCGTQYFF
                     DKAAIEGFKEETEKLSKLGLN"
     gene            complement(12775..13413)
                     /locus_tag="PXD71_000839"
     CDS             complement(12775..13413)
                     /locus_tag="PXD71_000839"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDL6290987.1"
                     /translation="MQNKIDWQKVDGLLPVIVQNAQTCEVLMLGYMNQQALEKTLAEK
                     RVTFYSRTKQRLWTKGETSGNFLNVVDMSLDCDNDTLLILANPIGETCHTGAESCFHQ
                     FEQEQPDWIFFSKLERMIAERKNADPESSYTAKLYAKGTPKIVQKVGEEAVESVIAAM
                     KNDREELVGEVADLMYHLTVLLHNADLSWAEINAKLKERHKGIGLHPEGSNK"
     gene            complement(13441..13827)
                     /locus_tag="PXD71_000840"
     CDS             complement(13441..13827)
                     /locus_tag="PXD71_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL6290988.1"
                     /translation="MIKITALDHLVLTVADMQKTIDFYTTILGMQEITFGDNRKALLF
                     GTQKINLHQKGAEILPNAQNADCGTADLCLLTVTPLQEVIYVLQRHQVEILEGGIVPR
                     TGAVGKIESVYCRDPDGNLIEISLYV"
     gene            complement(13824..14597)
                     /gene="hisF"
                     /locus_tag="PXD71_000841"
     CDS             complement(13824..14597)
                     /gene="hisF"
                     /locus_tag="PXD71_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDL6290989.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGA
                     DELVFYDITASSDSRTVDKSWVERVAQVIDIPFCVAGGIKTIADAEQIFAFGADKISI
                     NSPALANPELITELAKRFGVQAVVVGIDSWFEAETGKYWVNQYTGDEKRTRQTEWQLL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLVQLKKVREVCQVPLIASGGAGEMVHFRD
                     AFIEANVDGALAASVFHKQIINIGELKEYLAKEGVEVRR"
     gene            complement(14579..15328)
                     /gene="hisA"
                     /locus_tag="PXD71_000842"
     CDS             complement(14579..15328)
                     /gene="hisA"
                     /locus_tag="PXD71_000842"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDL6290990.1"
                     /translation="MQKSIIIPALDLIDGQVVRLHQGDYAKQTTYADDPIAQFADYLA
                     QGAEQLHLVDLTGAKNPAQRQTALIGKIIRETHCKIQVGGGIRTEQDVADLLAVGANR
                     VVIGSTAVKEREMVKGWFKKYGAEKFVLALDVNIDAIGQKIIAMSGWQEASGVSLEEL
                     IEDYQTVGLQHVLCTDISRDGTLAGSNVDLYREICAKYPEIQFQSSGGIGSLADIKAL
                     CGTGVAGVIVGRALLEGKFNVAEAIECWQNG"
     gene            complement(15498..16343)
                     /locus_tag="PXD71_000843"
     CDS             complement(15498..16343)
                     /locus_tag="PXD71_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HDL6290991.1"
                     /translation="MNEIKLFENSQIRSVWDSEKEEWFFSVVDIIEVLTQSSNPRRYW
                     SDLKRKIKDEEGGIELYEKIVQLKLKAPDGKMRETDATDLQGIFRIIQSVPSPKAEPL
                     KMWLAEVGKERIDEIIDPELTIDRALATYLKKGYSREWINQRLQAIQVRKELTDTWQD
                     HGVQVGREFAILTNEITQAWSGMTTRQYKDFKGLKKESLRDNMTTTELVLNMLAEAAT
                     KDIAQISHPQGLEENKTVAKRGGNVAKVARESLEAETGKPVITQQNAIDFAAVIEQVA
                     KQVKK"
     gene            complement(16442..17035)
                     /gene="hisH"
                     /locus_tag="PXD71_000844"
     CDS             complement(16442..17035)
                     /gene="hisH"
                     /locus_tag="PXD71_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDL6290992.1"
                     /translation="MTNLTIIDTGCANLSSVKFAFDRLGIRAEISRDLNKIQSADKLL
                     LPGVGTAAAAMAILHDRRLIDTIRNATQPMLGICLGMQLMTDFSHEGDVATLNLMSGK
                     TERLPECGLPLPHMGWNKVQYQADHPLFHDIEQGNHFYFVHSYGVLPNENTIATCDYG
                     VPFSAAIASQNFYGVQFHPERSGEAGAKLLRNFVENV"
BASE COUNT         4740 a         3971 c         3558 g         4845 t
ORIGIN      
        1 aaagaaatta acagtaatct aggaacacta acattaacta cgcaagaaga taaaaccggt
       61 atatttcctc cgacaatcaa attaatcaat ttttcatcca atatagaaga tgatattaaa
      121 gagattgagc aaagctgttt aaatagccga aagcaattat gggaaaaacg aagaaaaatg
      181 caagaggaac aaaaaattcc caattaatct tttagttgac cttaatttaa ataactaatt
      241 acctataaaa gcatttacgg caaagatctt ttttataaga ttaagctagt gaaaaaatta
      301 ttaaacaaga gaaaacaaaa ccaatgacaa aacaattcac aatggaagac cgttttaacc
      361 cttccgctgt agagcaagcg ctctaccaac actgggaatc gcaaggctat tttaagccga
      421 gtgaagatat taatgcaccg agctactgta tcgctattcc gccgccgaat gtgacgggga
      481 gtttgcatat ggggcacgct ttccagcaaa ccttgatgga tacgctgatt cgttttaacc
      541 gtatggaggg caacaatacc ctttggcaaa caggaactga ccacgcaggg attgccactc
      601 agatggtggt ggagcgtaaa atcgcagcgg aagaaggtaa aacccgccac gattatgggc
      661 gtgaagcctt tatcaacaaa atctgggatt ggaaagccta ttcgggcggc acgatcagcc
      721 agcaaatgcg tcgcttaggc aactcgattg actgggatcg tgagcgtttt acgatggacg
      781 aaggcttatc caatgcggta aaagaggtgt tcgttcgctt gcacgaagaa ggcttgattt
      841 accgtggcaa acgcctcgta aactgggatc cgaaactgca tactgcgatt tctgacttgg
      901 aagtggaaaa caaagaaagc aaaggctcgc tgtggcattt ccgctatccg ctggctaatg
      961 gtgcaaaaac cgcagacggc ttggattact tagtggtggc aacgacccgt cctgaaacga
     1021 tgttgggcga tactgcggtg gcggttcacc ctgaagatga gcgttatcaa gctctgattg
     1081 gcaaaagcgt aatcctaccg cttgccaacc gtgaaatccc gattattgcc gatgagtatg
     1141 tggatcgtga gttcggtacg ggcgtggtaa aaatcacccc tgcccacgat ttcaacgact
     1201 acgaagtggg caaacgccac ggcttgccga tggtgaatgt gatgacctta aatgccgata
     1261 ttcgtgcaga ggccgaaatt atcggcacag acggtaaacc gctttccgac tacaccgccc
     1321 caattcctgc cgactatcaa ggcttagagc gttttgccgc gcgtaagaaa attgtggcag
     1381 attttgaagc tcttgggctg ttagatcaaa tcaaaccaca cgacttaaaa gtgccgtatg
     1441 gcgaccgtgg tggcgtgccg attgagccga tgctgaccga ccaatggtat gtgtctgtca
     1501 aacctttggc ggaagtcgcg accaaagcgg tggaagacgg cgaaatccaa ttcgtgccga
     1561 aacagtacga aaacctgtat ttctcttgga tgcgtgatat tcaagactgg tgtatctctc
     1621 gccaattatg gtggggacac cgcattccag cgtggtatga cgagcagggc aatgtttatg
     1681 tagcacgaga tgaagcggaa gtgcgtgcga aacacaactt acctgcggac ttaccattaa
     1741 agcaagatga agacgtactg gatacttggt tctcgtctgg tctatggacg ttctcaacgt
     1801 taggctggcc agaacaaacc aaagaactga aaatgttcca cccgaccgat gtgctgatta
     1861 ccggttttga catcatcttc ttctgggtgg ctcgtatgat tatgttcacg atgcacttca
     1921 tcaaagatga aaacggcaaa ccgcaagtgc cgttcaaaac cgtgtatgta acaggcttga
     1981 tccgtgacga gcaagggcag aaaatgtcga aatcgaaagg gaacgtgtta gacccgattg
     2041 atatgattga cggcatcagt ctcgacgatt tgctcgaaaa acgtacaggt aatatgatgc
     2101 agccacaact ggcagaaaaa atcgctaagg caacccgcaa agaatttgcc gaagggattg
     2161 tggcacacgg cacagacgct ctgcgtttca ccctcaccgc ccttgccagc aacggtcgag
     2221 acatcaactg ggatatgaaa cgtttagaag gctaccgcaa cttctgcaac aaactgtgga
     2281 atgcgagccg ttatgtgctg accaatgaca agttggattt aagcgaaggc gacgtggaat
     2341 ttagccttgc cgatcgttgg attgaaagcc aattcaaccg cacggtggaa actttccgca
     2401 ccgcactcag ccaataccgt ttcgacttag tggcaaatgc gatctacgaa tttacttggg
     2461 atcaattctg cgactggtat ttagagctga ccaaacctat tttcttcaaa ggcacagatg
     2521 tacaacgccg tggcgcaagt cgcacgctgg tgaatgtatt ggaaaaactc ctgcgtttaa
     2581 tccacccagt aatgccgttc atcaccgaag aaatctggca gaaagtgaaa ggctttgtag
     2641 gtattgaagc cgataccatt atgttgcaaa aattcccaca atttgacccg cttgccattg
     2701 atgaaaccgc cgaaagtcaa atcaacttta tcaaagaagt gattgtggcg gtgcgtaaca
     2761 ttcgtgcgga aagcaacatc gcaccaagca aaggtttaga tttaatcgct cgtaatttct
     2821 ctgcggacga agtctctatt ctcaacgcta acgaagtgtt gctcaaatcg atggcaaaac
     2881 ttgatagcgt gaaagtgctg gaaaatggtg aaaatgcacc gctatcggta gcgaaactgg
     2941 tcgcaaatgg tgagatcttg atcccaatgg caggctttat caacaaagaa gctgagctag
     3001 ctcgcctcac caaagagatg gataaactta aaggtgaagt ggctcgcatt gaaggcaaac
     3061 tttcaaacga agcctttgtc gccaaagccc cagagcaagt catcgccaaa gagcgtgaaa
     3121 aaatgcaaga gtacctgagt ggattagaga aattgcaggt gcagtatcaa gagattgaag
     3181 cactgtaatt aactaacaag cggtcaggat ttggaaattt tttgctaatt cagaccgctt
     3241 gcttattgaa gcttttcatt ataaatgttt ggttaaaatc gcctctaggc ggtcatagcc
     3301 tgcttgggtg aagtgtaatc catcgtggca taaagttaga tctaaattcc cttctttgtc
     3361 agcaaaatat tcccacgttt ttacataagt caaatcagat ggaatatttg cctcaaaata
     3421 cgcattgagc tcttttattg cagcattatt ggtggtggta atattattaa ccggtgtggc
     3481 ttccaataga aaaaagttgg catttggatt aattttgcga agagaatcca caatttcgcc
     3541 aatccactct aacacttgtt ttggcgagta atccggctct tttacaatgt cattaacgcc
     3601 tagaaaaata aatacattat cgccaacgtg ttgaattcgc tcttgtcggg taatcacatc
     3661 caaatattgg cgggtgctgg tgcctgataa gcccaaattg gcaactgttt ttcctgctaa
     3721 attcggcgtg ccgttttcca tatcgcccca catatcaaac agggaatgcc caataaggga
     3781 aatatctgcc tgtttttcaa attctgattt ttttgtttgg tagcgattaa aaatatcttg
     3841 atcggtaagc ataggttctc cttaaatata aatgaaaaat cgctccatta ttatacaaac
     3901 ttgtaaacat tcactccaag atattttgaa gaaaaatcca ataaaaaagc cacttaatat
     3961 cgaattaagt ggctcaaaaa gattaattac acatcataag tggtcgatgc ggtattaccg
     4021 ccacgtcctg tccagttggt gtggaagaac tcaccgcgag ctttgtctgt acgctcataa
     4081 gtatgagcac caaagtagtc acgttgtgct tggagtaagt ttgccggtaa acgtgccgag
     4141 gtgtagccat ctaagaaggt aatggctgac gccatacaag gtacaggtaa accgatttcc
     4201 accgattttg ccactacttt acgccaatct gccaagcagt tttccaaaat atctttgaag
     4261 tatgggtctg agcctaagaa cactaaatat gggttggctt catacgcatc acgaatgttg
     4321 cctaagaaac ggctgcggat aatacaacct tcacgccata atagggcggt attgccgtag
     4381 ttgatgtccc aaccgaattg ctcagaagcc tcacgaatca acataaagcc ttgtgcataa
     4441 gagatgattt tggaagcaag taacgcacgg cgtaccgctt ctacccatac ttttttgtcg
     4501 ccttcaactt taccaatttc tttgttaaac aatcttgaag ccgcaacacg ttgatcttta
     4561 aacgcagata cgcaacgggc gaataccgac tcggtaatca gtgttaatgg aatgccgaaa
     4621 tcaagggcgt taataccagt ccatttaccc gttccttttt gacctgcggt gtcgagaatt
     4681 ttctctacca atggctcgcc gtcttcgtct ttgtaggcaa gaatatcagt ggtaatatcc
     4741 accaaatagc tgtctaattc ggtgtttttc cactctttaa agatttcgtg catttcatca
     4801 tagcttaaac ctaaaccatc ttttaagaat tggtaggctt cacaaattaa ctgcatatcg
     4861 ccatattcga taccgttgtg aaccattttc acaaaatgcc ctgctccgtc acgaccaacc
     4921 cagtcacaac aaggctcacc tttgtcggtt ttggctgaaa ttgcttggaa aatcggcttc
     4981 acatattgcc acgcagattc atcaccgccc ggcataattg acggcccgtg acgagcaccc
     5041 tcttcgccgc ccgatacacc tgagccgata aagcgaatgc ctttttccgc taacgctttc
     5101 acacggcggt tggtgtccgg atagtttgag ttaccgccat caatgatgat gtcgccttct
     5161 tctaaatgtg gcagaagagc ctcgataaac tgatccacca cctcaccggc acgcaccatt
     5221 agcatcactt tacgcggttt ttccaacttc gccgctaaat cttctaacga gtaagcaccg
     5281 ataatgttcg tgcctttcgc cgcaccttct aagaactcat ccacttttga agtagtgcgg
     5341 ttgtacgcca ccactttaaa gccgttgtca ttcatattca aaattaaatt ttgacccatt
     5401 acggcgagtc caatcacgcc gatatcgcct ttttgtgaca ttgtttgctc ctattgtttt
     5461 tgattctatg caagcggtcg aattggtaaa attttttgca aatttgaccg cttgtaacat
     5521 ttattaacct aaaataaact ccaccgccat ttggctgtgt aatgctgctg tatcgaaaaa
     5581 cggtaacgga ttgtcgtttt ggttaatcag caacccgatt tcggtacaac ctaaaatcac
     5641 accttctgca ccttgttcac taagttgttg aatagtctga agataaaacg ctttggcact
     5701 ttcagtaatc ttgcctaaac agagttcctc gaaaataatg cgatgaattt cttgttgcgt
     5761 ttcctcatct ggtactattg atgtgatacc acgctccaat aaaccgtctt tgtaaaaatt
     5821 atcactcata gtaaattttg tgccaaggag tgctactttc tgcagacctt gagctgaaat
     5881 cgcctcagcc acacagtcca aaatatgcaa aaacggcaca gagatatggc tactaatttg
     5941 atcggcaatt ttgtgcattg tgttggttgc caataaaatg ccttctgctc cggcttgttc
     6001 tagcaatcta gcttgttcag ataaaatttc agcggctgct tgccaatttc cctgcttttg
     6061 gcattgtaca atctcttcaa aattgacact cgaaattaaa atcttggcac tgctatttcc
     6121 acctaatact ttattcaccg agcgattaat atctaaataa taagaaaccg tgctttccgc
     6181 actcattcca cctaaaatgc cgagtgtttt cattaatata ccttattgat tttccgctaa
     6241 cttttctaaa atgcggctca ttgataaatc cattttagaa aaagcgaacc atttcttacc
     6301 taaatcttta agtgatgcac cgatatgata aagctctttt tcatcaataa tcaaaaatcg
     6361 ctcgtgagat ttatcaaata ttttcacatt aatgtgagca tattggctgt tatatttttc
     6421 caaatctaat gcaagttgct tagatatttg tttcgtataa atcgtaactg atacgttatg
     6481 cgatcgtttc gtaaataaag ttaaaacggt atcatctaca tagttatcaa ttaaaatcag
     6541 cgatgatttt gcacctctca ctaattccgt aacaaactga taagcgttaa aggtttcacc
     6601 ttcaaagaaa accccttgca ttttaggctt attgccttca agagattgaa acacctgctc
     6661 taactgccca gacatctcca actgtttacg ctctacactt tctaagcgtt gtaccaaacc
     6721 actatttgtt tgcaaaagat gacgcatttg cacaaaggct tgcataatct taatgctaac
     6781 ttgcaccgca acttcactac gcaatactgc cgaaagcatt gcaatgcctt gctcggtaaa
     6841 gacataagga agatatttcc tatgttggcc acgcccacta ttttcgttta agatcacaaa
     6901 ttgtgatctt aaagatagcc attcgttttc tgttaattga aaacgaaata tgtcaggaaa
     6961 tcgctcttca ttccgtttta ctgcttggtt aaaggttcga gtatctacct gataaagctc
     7021 tgccaaatga acatctagca ttacttgctc gccacgaata gtaaaaattt tagagttaat
     7081 gtttggttca attaattgaa ttggattcat ctttcttcct tattcttcag tgaagatcac
     7141 ttttgtgatc tcaaaataag aaagactatg ccaatttttg gcctatacca cgagcagatt
     7201 tttctgcttt ttcgatgaat atcacaaaaa tactttgcaa catttttcta aaatttaacc
     7261 gcttgtaaag gtcactgatt gtgacctaga gtaagttagc cgcctcacta tccaaatacc
     7321 attccgttac cccattttcc gcctgaattt tggcggcagg ataaggtaaa ttttctgccg
     7381 gagtggtttg gatttctttt aaaatttccg ctttgcttgc tcctgtgacc aagtaagtga
     7441 tccgtttggc tttttcaatt aaagatgccg ttttggaaat gcggatctgc cctgtttctg
     7501 ggtatttggc gatcacggca acgttctgat cattaaaatc tgtttgatgt gggaacagtg
     7561 aggcggtatg cccgtctgtt cccattccta aaataatcca atcaaacgcc aagttcggca
     7621 cacaagcggt caattctgcc gaaaatcttg caagttcttg ttctacattc tcttcaccac
     7681 gaatgcggtg aatattttcg cttggaatgt cgatgtgatc gaacagtaat ttctgcactt
     7741 cgccgtagtt actttcggga tcggttgggg caaccattcg atcatccccc caccagaaat
     7801 gcaggttttg ccattgaatt tcggtattaa acggtgcttg agctaaggtt ttgaataata
     7861 atttgggggt tgaaccaccg gaaagggaaa tatgcaccgg gcggttttga tggctgtatt
     7921 caataaactc tttagcaatt tgttcaaccg cagcttgggc ggtggggaaa ataactttat
     7981 tcataatttg cctcgttaga tcacccctct ttagaaaaga ggggaggggg agatttgatt
     8041 acgatttcaa tatttcgtat gagcgtatat aacttctgcc aaatctcccc tagcccctct
     8101 ttgctaaaga ggggaactga aatttacact ttcttcttca ttgtaccgga cggttttcgc
     8161 catactttgc cgtgtctggc gattaattta tctgcctcgg ttggccccca agtgccggat
     8221 tcatattcat acacacgccc acgattagct ttgtaggcta aaatcggttg cacaaatttc
     8281 caacaagcgt gtacagcatc agtacgggca aataaggttg catcaccttt aagtgcgtct
     8341 aatagtaagc gttcataagc ggtgagtaag cttgatgatg aacttaaatc agaatagcgg
     8401 aaatccattg agacttcttt cgcttcaaag cctgctcccg gttttttcaa gccgaaacgc
     8461 attgaaatgc cttcgtccgg ttgcacacga ataatcaatt tattttccgg agcattttgg
     8521 ctaaataccg ggtgtggtgt agttttaaaa tgaattacga tttccgtcac acgggttggt
     8581 aagcgtttac cggttcgcac atagaacggc acacctgccc aacgccagtt atcgatttta
     8641 cattttaccg ccataaaggt ttcagtgttg gaatcagccg gcacgccttt ttcttccaag
     8701 taagccggca cttctttgcc gtctaccgtg ccacgagcat actgacctaa caccacattg
     8761 tgtttaacgt cttcttcagt taacggatgc aagcaatata gcacttttgc gacttcatca
     8821 cgcattgaat tagcattaat aattgccggt ggctccattg caaccatggc taatacttgc
     8881 aataagtggt tttggaacat atcacgcatt gcccccgagc catcatagta accgccacga
     8941 tcttcaacgc cgatggattc tgccccggta atttccacat aatcaataaa attacggttc
     9001 caaagcggtt caaataagcc gttagaaaaa cgtaaaacga gtaagttttg taccgtttct
     9061 ttacctaaat agtggtcgat acggtagatt tgatgctctt caaagcattt gtggatttgt
     9121 acgtctaatt ttttggcggt ttcgatatcg tagccgaatg gtttttcaac aataatccgt
     9181 ttccagccaa actcttcggt agttaagccg tgagcggcta aacattccgg aatcacgcca
     9241 tataaacttg gtggcgtgga gaggtaataa agcgtgttac cggctgtttg atatttatcg
     9301 tgtagctcat caagacgagg cagcaacttg gcataatcca ctgcatcaga ggtatttaca
     9361 gcttggtaat aaagatgttc gcaaaattta tctaattctt caccgcttgc tttttcaaat
     9421 ttgactaacg catcacgcat tttttggcgg aaagattcgt cggttaattc tgaacgagaa
     9481 acacctagga cggaaaaatg ctcgcccaat ctgccaatct tatataagtt ataaagagca
     9541 ggaattaatt tacgaaaagt taagtcaccg gaagcaccaa aaatgacgat acagctattt
     9601 tcagcattca tttatttatc ctattgctaa ttgttaaaat tttgttgggt gcaagattat
     9661 cataaaaatt gctaatcgga actacctatt tgaataaaaa tattttatcc caatctatcg
     9721 agccgtcagc caccatcaca aaataaggat taatcaaact ttctcgctga ttataagtca
     9781 gcggcgaaaa attaaagtcg aaaattttgc cgcccatttc acttaacagc acttctgcca
     9841 cagcagtatc ccattcgccg gtgtcaccaa aacggatata acaatcggct ttcccttctg
     9901 ccactaaacc ggcttttaga ctgcccgaac catattttac acactcaatt tgtggggctg
     9961 aaatggcatt tttagcttgt tctgcatgta aatcgctgcc tacggtaaca atcaaaaggg
    10021 cattctccgc acgtttcggt ttgacaagcg gtcgaatttc tccattttct tgcaaaaagc
    10081 taccgctacc ttccatacta aaataggttt ttcctaatat cggagcatga ataacgccca
    10141 gcaccggctg atttctaccc tcacgttttt gcactaacgc aatcaccact gaaaattgac
    10201 cggtgcggtt aataaactgt tgtgtgccgt caatcgggtc gataatccaa tattcttgcc
    10261 attgttggcg ttcggcaaac ggaatatcgc aaaactcttc cgataatacc ggaatatctg
    10321 gtgtgagttg cttcaagcgt tcagttaaaa attggctgat aatcagatcc gcttccgtca
    10381 ccggtgtgca atcggctttg attttaattg ccaccgagcg ggcataaagc tgctttaact
    10441 gctcaccggc tgctttggca atattgataa tattctcgag taggggttga gttagcgttt
    10501 gcataatagc tccttgccta tttttttaag tattctcgta gcataaatag agcggacaaa
    10561 ttccgtgctt cggcaaaatc aggatctgcc aataattgat caattttatc caacggataa
    10621 cgcacaattt ccagcggttc aggctcatcg ccttccaatt ttgatgggta cagatcttgt
    10681 gccaaaaaca ggtgcattaa gccgtacata tggcttggcg aactgtaaag cgaccgcaga
    10741 aaatctaatt ttttcgcccc aaaaccaatt tcttcttgta attcacggtt cgcactctca
    10801 atcggctctt cacctgcatc cacaatgcct ttcggaaaac ccagctcgta acgctccgag
    10861 ccgaccgcat actctttcac aaaaatcagc tcgttgtttt gaatcggaat caccattacc
    10921 gaagaacggc gattcggcgt aagtcgttcg taagttcgct cttcgccatt agaaaagcgt
    10981 aaatcgaccg cttgcacctc aaaaatgcga gatttcgcta ctgttttaat gttcaaaatt
    11041 tcaggaagtt gttttatcat tttatctcac ctttttgagt tattttagtt gaaaaatctc
    11101 actttcgctc taatatttcg actattgttt tgattttaag gcagaaaatg aaaaataaac
    11161 acgaaaaaga acaaaacgac gccgaagttc ggctagataa atggctttgg gcggctcgtt
    11221 tttataaaac ccgttcgatt gccaaagcaa tgattgaagg gggtaaagtc cattataatg
    11281 ggcaaagagc taaagttagc aaagcggtgg agatcggtgc catcatcaaa cttcgccaag
    11341 gcaatgaaga aaaagaggtg gaagtgttag ccctcagtga ccaacgccga ggggctccgg
    11401 aagcccaact gctctacagt gaaaccgaaa aaagcattaa acaccgtgag gcaatggcat
    11461 ttgcccgcaa agcgaatgct ctttcaatgc cacaccctga tcgccgtcca aacaaaaaag
    11521 aacggcggga tttaatcaaa ttcaaagagc aagaatttag ctaagccctt gcaatcagaa
    11581 aaataatccc tatattgaat atcctaagta ggggcgaaaa atttttcgcc cctatataat
    11641 tatttcgggc gaacattgat tcgcccctac agcgttcgat ttgcacattt ttagccacaa
    11701 aatgcaatgc attttgaacg ttggctttgc caacggctcc gtaggagcga acaaaattag
    11761 aaataatttt gtgagtaata atcgaccgct tgcaacgagg aatcaaaatg aattacacca
    11821 aagacaatga caaactctac cgctatttat tcaaagaccg agcggtacgg ggcgaatggg
    11881 tgcgtttaaa caacaccttt accgagacct taaacaccca tcactaccct aaagcagtac
    11941 aaaatttact cggagaaatg ttagtcgcaa ccagtttgct gactgcaaca atgaaattta
    12001 acggcaacat tactgtgcag attcaaggtg acagcccgtt aaaattggcg gttgtgaacg
    12061 gtaacgacca acagcaactc cgtgctttag cccgtgttca ggcagatatt gcagacacag
    12121 cctcgttagc ggaaatgatc ggcaatggtg tattagtgat ttctattatc ccaaccgatg
    12181 gtgaacgtta tcaaggggtg attgctctcg ataaaccaac cattcgtgaa tgtttagaag
    12241 attacttcgc ccgctccgag caattacaaa cccacctcgt tatccgcaca ggcgaatttg
    12301 aaggcaaagc agtggcaggc ggtatgttgc tacaaattat gccggacggc acaggctcaa
    12361 gagatgattt tgagcattta gccacgctca ctgaaaccgt aaaagatgaa gagttatttg
    12421 gtttagaggc agaagaattg ctctatcgcc tctaccacga agagcaagta gaagtttacc
    12481 caccacaaga tactgaattt aaatgtggct gctcaagaga gcgttcaggc tccgcattat
    12541 tactggtgcc ggcagaagaa atcgaagaaa tgttggagga aaaacaaggc gtgatcgata
    12601 tgcaatgtga atgctgcggg acacaatatt tctttgacaa agcggcgatt gaaggcttta
    12661 aggaagaaac tgaaaaatta agtaagttag ggttaaatta agctaaacaa gcggtcaagt
    12721 ttcttgtctt ttttgcaatt tgtaaaaaaa cggtgaaatt tgaccgcttg cattttactt
    12781 atttgagcct tctgggtgta acccaatccc tttgtggcgt tcttttagtt tagcattgat
    12841 ctctgcccac gagagatcgg cattgtgcaa taacaccgtt aagtgataca tcagatcagc
    12901 cacttcaccg accaactctt cccgatcatt tttcattgcc gcaatcacgc tttctactgc
    12961 ctcttcccct actttttgga cgatttttgg cgtgcctttg gcgtaaagtt tagcggtata
    13021 agaactttcc ggatcagcat ttttacgttc ggctatcatt cgttctaatt tgctgaaaaa
    13081 gatccaatct ggttgttctt gttcaaactg atgaaaacag ctctccgccc ccgtatggca
    13141 ggtttcgccg atagggttag cgaggattaa cagggtgtcg ttatcacaat ccaaactcat
    13201 atccaccacg tttaggaaat tgcccgaggt ttcgcctttc gtccacagcc gttgtttagt
    13261 acgggagtaa aaagtcactc gcttttcagc cagcgttttt tcgagggctt gttggttcat
    13321 atatcctaac atcagtactt cgcaggtttg ggcgttttgg acgataacgg gcagtaagcc
    13381 gtccactttt tgccagtcta ttttattttg cattttttct tccttttttt gaccgcttgt
    13441 ttagacatat aggcttattt caatcaaatt accatcaggg tcacggcaat aaaccgactc
    13501 aatttttccc actgcccccg tacgaggcac aatcccacct tccaaaatct ccacctgatg
    13561 tcgctgtaac acatagataa cctcctgaag tggagttacc gttaagaggc ataaatctgc
    13621 cgtgccacaa tcggcatttt gggcattggg taggatttct gcaccttttt ggtggaggtt
    13681 gattttctgt gtgccaaaca aaagcgcttt gcgattatcg ccaaaggtga tttcttgcat
    13741 acccaaaatg gtcgtataaa aatcaatggt tttttgcata tcggcaacgg ttaaaaccag
    13801 atgatctaag gcagtaatct taatcatcta cgcacctcaa ccccttcctt cgccaaatat
    13861 tccttcaact ccccaatatt gataatttgt ttatgaaaca cactcgctgc caacgcacca
    13921 tccacatttg cctcaataaa agcatcacgg aaatgcacca tttcgcctgc accgcctgag
    13981 gcgatgagtg ggacttggca aacttcacgc acttttttca gttgcaccaa atcgtagccg
    14041 ttgcgtacgc cgtcttggtt catcatattc agcacgattt cgcctgctcc tcgcttttgc
    14101 acttcctcca cccaatccaa caattgccat tcggtttggc gggtgcgttt ttcatcgccg
    14161 gtatattgat tgacccaata tttgccggtt tctgcctcga accagctatc aataccaacc
    14221 accaccgcct gcacgccaaa gcgctttgcc agttcggtaa tcagctcggg gttggcaagg
    14281 gctggggaat tgatggagat tttgtccgct ccgaaagcga aaatctgctc cgcatcggca
    14341 atggttttga ttccgcctgc cacacagaac ggaatatcaa tcacctgtgc cacacgttcc
    14401 acccagcttt tatccaccgt tctgctgtca cttgaggcgg taatatcgta aaacaccaac
    14461 tcatccgccc cctcttgggc ataacgctgg gcgagtggca caatgtcgcc gatgatttcg
    14521 tggttgcgga actgcacgcc tttcacaact tgcccgtcac gcacgtctaa acaaggaatt
    14581 atccgttttg ccaacattcg attgcctccg ctacattaaa tttaccttcc aacaacgcac
    14641 gaccgacaat cacgccagcc acgcccgtac cacaaagcgc tttgatgtcg gcaagcgagc
    14701 cgataccgcc tgaggactga aattgaatct ctgggtattt ggcacagatt tctcgataga
    14761 gatctacatt tgaacctgcc agcgtgccgt ctcgggaaat gtcggtgcat aaaacgtgtt
    14821 gcaagcccac ggtttgataa tcttcaatca actcctcgag cgacacacca cttgcctctt
    14881 gccagccgct catcgcaatg attttttgac cgattgcatc aatgttcaca tccagtgcca
    14941 gcacaaattt ctccgcaccg tactttttga accagccttt aaccatttca cgctctttta
    15001 ccgcagtcga accaatcacc acacggtttg ctccaacagc aagtaaatcc gccacatcct
    15061 gctcggtgcg gataccgccg cccacttgaa ttttgcagtg ggtttcacga ataattttgc
    15121 cgataagtgc ggtctgtctt tgagccggat ttttcgcccc tgtaagatcg accaaatgta
    15181 actgctccgc cccttgtgcc aagtagtcgg caaattgggc aatcgggtca tcggcataag
    15241 tcgtctgttt cgcataatcg ccctgatgta ggcgaactac ttgcccatcg ataaggtcga
    15301 gggcggggat aatgatggat ttttgcatat tattgttcct gtgtgcctag cacgagccgt
    15361 gctaggctga ataatcccag tcgcgctgcg actgcctctc taagctacgg agtagcttgg
    15421 tttatctagc cccatacggc tcgtatgggg tacaagcggt caaattcttc caaaaacttg
    15481 caaaaaaccg accgcactta tttcttcacc tgtttcgcca cttgttcaat caccgcagcg
    15541 aaatcaatcg cattttgttg cgtaatcacc ggtttgccgg tctctgcctc caaactttcc
    15601 cgagccactt ttgcaacatt tccgccccgt ttggcaacgg ttttattttc ttccaagccc
    15661 tgtgggtggc tgatttgagc gatgtctttg gtggcagcct cagcaagcat attcagcacc
    15721 aactcggtgg tcgtcatatt gtctcgcaga ctttcctttt tcagcccttt gaaatcctta
    15781 tattggcgag tagtcatacc gctccacgcc tgtgtgattt cattggtgag aatcgcaaat
    15841 tctctgccca cttgcacacc gtgatcttgc caagtatccg tcagttcttt tcgcacctga
    15901 atggcttgca agcgttggtt aatccactca cgggaatatc cctttttcaa gtaagtagcc
    15961 aaagctcggt caatggtcaa ttcaggatcg ataatctcat caatccgctc tttgcctacc
    16021 tctgccagcc acattttcaa cggctcggct ttcgggcttg gcacagactg aataatacgg
    16081 aaaatgcctt gtaaatccgt ggcatcggtt tcccgcattt ttccgtctgg ggcttttaat
    16141 ttcaactgta cgattttttc gtacagttca attccgccct cttcgtcctt gattttccgc
    16201 tttaaatcac tccaataacg gcgtggattg ctactttgcg tcagcacctc gataatgtcc
    16261 accaccgaga aaaaccactc ttctttttcg ctatcccaca ccgagcggat ttggctgttt
    16321 tcaaatagtt taatttcgtt catttctcac cttctttcac tggttaaatt aacagtaatt
    16381 ttgtcgaaat aaagcgagaa atcaagccct attttcaaac ttttcgatcg agatcacaaa
    16441 atcagacatt ctccacaaaa ttcctgagca acttcgcccc agcctcgccc gaacgctcgg
    16501 ggtggaactg tacgccgtag aagttttggc tggcaatggc agcggaaaac ggcacgccgt
    16561 agtcgcaggt ggcgatggtg ttctcattcg gtagcacacc gtagctatgc acaaaataga
    16621 aatgattgcc ttgctcaatg tcgtgaaata gcggatggtc ggcttggtat tgcaccttgt
    16681 tccagcccat atgcggcaac ggcaaaccgc attcaggcaa gcgttcggtt ttgccactca
    16741 ttaagttcag tgttgccaca tcgccttcgt gggaaaagtc cgtcattaac tgcattccta
    16801 agcagatgcc aagcatcggc tgggtggcgt tgcgaatggt gtcgataagt cggcgatcgt
    16861 gcaagatcgc catcgccgcc gctgctgttc ccacaccggg gagtagcaat ttatcggcag
    16921 attggatttt gttgagatct cgcgagatct cagcccgaat gccaagacgg tcaaaagcaa
    16981 atttgaccga agacaggttg gcacagcctg tgtcgatgat ggtgaggttg gtcatttagt
    17041 ttcctgagtt aagtatttct tatagtgaag taaaattgac tcaatatatc tgtttccttc
    17101 aaaagctttc ttat
//
LOCUS       DANLXS010000033        16990 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.033, whole genome shotgun sequence.
ACCESSION   DANLXS010000033 DANLXS010000000
VERSION     DANLXS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16990
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.033"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(58..9702)
                     /locus_tag="PXD71_000845"
     CDS             complement(58..9702)
                     /locus_tag="PXD71_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDL6290993.1"
                     /translation="MNKHCFRTIFSKTLQRIVVVSELAKLAGKSESESMTSGAILQKI
                     CKIRPLAFSLFCALGFVTLSDNAMAETLIIQADSTAPKNQQPIILQTANGLPQINIQT
                     PNDKGLSHNKYSQFDVAEKGAILNNSRTNTATQQAGMVQGNPYLARGEAKVILNEVNS
                     SKPSVMKGYVEVAGKKAEVIIANPSGLHCDGCGIINADRATLTTGKPQINQGNLEGFV
                     VEKGKVTVAGKGLDNSRVDYTDILAREAKINAGVWSKKETKVITGKNTVKRSNSDNNL
                     QIIHTNQPLAGEEKPTVAIDVGELGGMYAGKIHLIGTEQGVGVRNAGHIGASADTLTI
                     DSQGRIVNSGTFNAQRQVQLNAQHGIDNQGKIENKQGNIQLNSKADIQNSGSIVARGG
                     NIQKKAETKIQQSGETVAKGNITYTARQIHADKNSLIAAGVDVTDTAQGEVRKLEAQS
                     VAGKTIQLTAPEKTTVQGKNIASGKINVAAAEIDANQSQNNAHTVEYSAKSGDIQANS
                     AQFSANQIKLTTPKWLSTEGSQLTAEVIEAQQANLNARGSVWKQTGEQDFRLKAESIH
                     TDSARFSVGGHFEIDGNRLTNNQGLLSSGKRLGLNLNDAVDSVDGKLVAKGELSIRAP
                     LLNNQQGLILSGEKQAITVNTLKNQSGVITSQTEQTLDITSELQNQGGRISASGVAIN
                     AQSLDNREQGEISSVKELALTVSQQVDNRHGVIQSVGNLTLNSTLLDNQGGVLKSATD
                     ITLNVPTVLNNRISENGSLIEAGGSLTMNSFEVENQGTLARQPQPIQGIIASQFVLNS
                     TKQLNNEKGGIYIGSSARFTLSDSLNNRNGEILSWGDLAIFGGKSLTMTNTEGKVSAN
                     NGLEITAKALSGDGDVQAGDIRLNLQENFDITKDINANRSMIIQTEGDILNRKKLAAN
                     DRLQLTANQITNAQNARISSADTRLTATSTVYNEGLINSRSDNDNAQTVIKAQEIHNI
                     GTGNIYGDKVALGAERILNQDKDGTSATIAARQSLGLAAKVIENNTLVYEANKKSGSL
                     IYSGGEIAIGRQLNEQDTVIGNAGQLHNRSSIIEAEGRIQLNADKVANVNSHYRSELE
                     TTDRQTVNEHFILPRDKHNRGGSDVYDNGHLPTINVNQLRKGYYRKTWSYWQKYDEAN
                     LPVITDISQLNATTVLAKPYAQSCANDESVDCTLLPAGNYKKDNPVWTWFNLTPPAED
                     APEMTPEIIQAMNEEDLANQHLSEAEQEKLQKEQGQGIAPVVPARPIEPVRGSTETEA
                     QYQKRLQTYQAELKAYEAAEAAYQRYVLLKPLIDWEAKYGANIQALDEAIAKHNKANL
                     SQEYPHFWNLWIKERMVKENITKASLPAQILAGGDLTYQSDEFINDKSWVIAGKALNP
                     IGRSRIANLDDADAVRQDWELGRRQFSFTEYHGGTRRRHSRYDENDGPLARLKEVHKD
                     MNIFVELENTSPTAYTDYVDAKAANAVKPEGNQVERGNLPDAPNPPTMAIERIPSPNA
                     AAFEVRSMTVDTRLPNQGLYRINPQADSHVLIETDPDFTDKKRWLSSDYMFNALRHDH
                     NNVHKRLGDGFYEQRLIREQINRLTGRQFLGDYTDFDSQYKALMDAGITFAQKFNLRL
                     GITLSPEQVASLTSDIVWFETQTVRLPSGETVSVLVPKVYVLARKGDIDGKGSLISAD
                     ALTINSKEIVNQGTIAGRRLAQFNAERLENSGTLSGGVLAAKVSGDLENIGGKIEADK
                     AILLDIAGNFNHTSTTRTTQIDLDGFKRAETNLDRKALLHVKDENGLLQVQAGNITSA
                     GADIINDGQGQTYLSAKNQLNLTALQVGFDEKMGGGNHYRNEALNDVVISRVQGKGDV
                     VVSGKNLYSEGADLDAKARLALLAENDMVLGSATRSRDYEEYHKTKSGGALAKKTKTT
                     YDNISETEHKGTELTGEDILVKAGHDIQGESLLAVSRSGDIDIIGGNNVTLSSATNQL
                     SEQHIRETKRSGFLNGGEIGFTIGSQKTRQENSLEGAIQSTARNTLGSEGGNIRIQAG
                     NKASVSNVDVLISNEKTAVISGKNGVRVESGKDVINSTDNYEFSQSGLSIALSTPVTD
                     AVQSARQSVQKAKATQNEKLKGVYAVKAAEDAVIAAQNAQKVAETLGNLGNALTENHA
                     AAENPAVKISVSLGTQKQQRESRSQSVTHSKSTLNGGNIALISGAGKVALEGVDTRVK
                     DTLLLDGKLGIESKGVADTYQNNTKNKNHSASVGVFIGLDRDSFGIGFEAGVSGGKGK
                     ENIETETWQNNQLQAGKIVTNSANGKLMLDATNVKANHWEGEVQDFEARSRQDISKYK
                     SEYTEGSVHGTLAVGTTGVNGHVAYNGAKLNTAQVENQTAIDIGKSGMDVKVKKNAHF
                     EGAVMTSQAEKENNRFQAGTLTTGDIENHSELKTRSVAISGGTSGVNPMSALSLLGNK
                     NESERSTTKAAIGENIAITLTEDLNAETTLNHLNRDTQNANQKVTKYDISEVKETQEL
                     VKGIGEIAEKAYQIYTHSEREKVTEAKLELGKAQAQKASKEEIGKLEANLDRLQKEFD
                     KNYGTGSKTKRAVEAVTAALQGLAAKDVGQAAVGLASPYLNAEIKKYTEGDTQANLIA
                     HALLGAVEAAATGNNVLAGAAAGAGSEAAADFIVKTLYKGKSTQDLTEAEKQNVALLS
                     QLASGLASGLIGDSTQSAAVGADIGKRAVENNYLSNWQEQKRSQELKSCHGDARCENQ
                     VRLYWGLVDIGQDVSFVSGAVAGVPENLAESVEGLISMFSSPIETIVAMKELIAREDT
                     LSYIANMTKQEYLAKLDKMQVYYEQAGAVGSFNAGKEFGNMMTDVVTALTGAGALVKT
                     GVGTTAKIVTKSGKELAIPKTRSSASLGTCSFHGDMEVKTDKGYRQISSIKVGDKVLA
                     KNERTGITTYQKVQAHYSNPYDYTVYVEVADSKGKYHTIVSNKIHPFFTQVLSGRVPI
                     SSEGHSYNGEIAKAQWVDAQHLQKGYRLLAESGEWQTVTKVKIKQERLKAYNMTVEKD
                     HTYFIRGTNADNEGVWVHNDCWHALPNDAKQVKNIDGYKAYQFTDPKDGKVVTVVQKD
                     LNRFETPNHQAGVDPHFNRLSQQIDAETGRYLSTDNPVDGLYNRAYLRSETLQKIYAN
                     YEKLPNGNYRNKLTRQEIEQPIDIGHMYGWEHRRLSLAAKELNWSQKQFNDYVNARPE
                     NFRLENMSENRSHKNEMPGKHDIQRIKSDMEKFLKEGK"
     gene            complement(9754..11529)
                     /locus_tag="PXD71_000846"
     CDS             complement(9754..11529)
                     /locus_tag="PXD71_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL6290994.1"
                     /translation="MKTPQNLILSTVLLSASFVAEANSSSLVQQARIDATQQQIDAAQ
                     QHRQQQYQQNQAEKFQPKADVRLETQINDTLTLSNHELPCYPIHQITLIDYSPDNSLT
                     TSQFQWAFEKAVKSFHLTLPHCFGGEGLAVLMKQIQNEIIGKGYVTTRVVTAEQDLRG
                     GKLVLTVIPGKIRNTLVVDSGKVPRFTKLHALTGFTFAEGDILNVRDIEQSLENLKRV
                     PTAEANIEILPSSSEKAEVGDSDLKISYSQAFPFRLNLGLDDAGSKSTGKLQTSATLS
                     IDNIFSANDLFYTSFTHSMKQKGDDNGRRASKNLTLYYSVPFGYWQLAFSHTHHRYHQ
                     EVFGAFDKSYLYAGESDTDKLTFSYLLYRDAKRKTSLSGSLWPRQSQNYIDGSEIEVQ
                     KRRMAGWEAGFSHKEYFPTATLELSANFKRGTAARGALSAPEEDYGEGTSRPKIITAS
                     ASLTKPFFWGKQPWQWQSSLNAQWNKTPLVPQDRFSIGGRYTIRGFDGELTLSGERGW
                     NWRNELSWQFNPNHAFYWAVDGGRVSGWATENQLGHHLMGTAIGFRGGVKGFSYDVFA
                     GRPMRKPEGFKTSDTVAGFNIGYNF"
     gene            11684..12769
                     /gene="queA"
                     /locus_tag="PXD71_000847"
     CDS             11684..12769
                     /gene="queA"
                     /locus_tag="PXD71_000847"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL6290995.1"
                     /translation="MLVSDFHFNLPDELIARYPTAERSASRLLHLNGETGEFADQQFV
                     DLYDHINEGDLLIFNNTRVIPARLYGRKPSGGKVEALVERVLDEHRCLAHVKASKAPK
                     EGAELIFGEDKLGEGKGFTATMVARHDTLFELVFNEEQPLFELLQQAGHMPLPPYIDR
                     PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNAMLEKLKAKGVDTAFVTLHVGAGTFQ
                     PVRVETIEDHKMHAEYAEVSQEVVDKILATKAKGKRVIAIGTTSVRSVESAAQAAEKD
                     GKLIAPFFSDTAIFLYPGKTFKVIDALVTNFHLPESTLIMLVSAFAGYRNTMNAYAHA
                     VETKYRFFSYGDAMFINKNPNALSDVP"
     gene            complement(12796..13334)
                     /locus_tag="PXD71_000848"
                     /pseudo
     CDS             complement(12796..13334)
                     /locus_tag="PXD71_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13347..15629)
                     /locus_tag="PXD71_000849"
     CDS             complement(13347..15629)
                     /locus_tag="PXD71_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL6290996.1"
                     /translation="MTLDRFCIALILFHLPILWLPKAFLFVGIVIGTLVLILSVYLRS
                     KLTAVLGLLVCIGYIQIIQVAKNAEKTTASKTPVQFEISKILKQTDYQTAIATLNTGE
                     KIYLNWQSKQPLLLNQFYQAELALRPISARSNIGNFDRQRWYFANHINMTATVRKVET
                     LAKTDYSLRTNWLNKVKAETDALPTQGFLLALAFGERAWLKTEHWGQFQQTATAHLIA
                     ISGLHIGLAMLFGVYFAKGVQWLCLKSELTALQAVGFSLYFARIVSFITAFGYSYLAG
                     FAVPTARALLAIAFVLLCQVLRRHYTPWQYWGRIVALLILIDPLSLLSDSFWLSILAV
                     ASLILWYQYFPLSRFLNDEYRKKITKFNRLWLSLLHLQIGIWLIFSPVQLYFFEGISA
                     FALLANLIIVPLYSFLLVPLILFTLLTDNLFSTWLLADYLAQFSLWLLDPFSNAWFAL
                     SHWQQWQLLSLNLLVLVLLYCKLYRLSYKKWLSAVAVSLLFNLSFYLPKLFNQTQTEW
                     IMFDVGQGLAMAFIYQQNKAVIYDTGSSWQANDGSRNSMAKMEILPYLKRNGIEVEAI
                     FLSHDDNDHSGGVVELLQAYPKARLISSSKVTYHNINPEPCIAGTSWQFGEWQLLAIY
                     PSHIAERAKNQDSCVILAKNDRLKILLTGDSGVEQERQFAHQLGKIDFLQVGHHGSKS
                     STGETLLSITQPAFAVISAGRWNPWKLPNKQIIERLNRRHINVLNTVETGMIRVKFYP
                     SSIEIEQARGAFSPWYKQFY"
     gene            complement(15771..16985)
                     /gene="sstT"
                     /locus_tag="PXD71_000850"
     CDS             complement(15771..16985)
                     /gene="sstT"
                     /locus_tag="PXD71_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL6290997.1"
                     /translation="MSQSLSSKILGGNLVLRIAIGLVLGVLLAFINKEWATSVGVLGQ
                     FFVKSLRAIAPILVFVLVLAAIANKEVGSDSKLKPILVLYVLGTFFAALTAVVLSFMF
                     PTTLELTASPDGLTPPQGIGEILKTVIFNLVDNPLVALSNANFIGILAWSIGLGIAFR
                     HGSASSKAFLNDLANAVSFVVKVVIAFAPIGVFGLVAETVAINGIDAFEDYARLLVVL
                     VGAMLFVALVLNPLLVYWKIRRNPYPLTFTCLRESGVTAFFTRSSAANIPVNMNLAKR
                     LGVRDEIASVAIPLGASINMAGAAITVTVLTLAAAYTQGIQPDFATALLLSVVASICA
                     CGASGVAGGSLLLIPLACSLFNIPNDIAAQVIGVGFVIGVIQDSVETALNSSTDVLFT
                     AAVSYAEDQKSA"
BASE COUNT         4354 a         4002 c         3394 g         5240 t
ORIGIN      
        1 gcaatggcaa ctagcattga gggttcatct ttatattttt catataattc attcatatta
       61 tttaccttct tttaagaatt tttccatatc ggatttgatt cgttgaatat catgttttcc
      121 tggcatctca ttcttatggc tacgattttc agacatattt tctaatctga aattctccgg
      181 tctcgcatta acataatcat taaattgttt ttgactccaa tttaattctt ttgccgctaa
      241 tgataatcgg cgatgttccc agccatacat atgacctata tcaataggct gttcaatttc
      301 ttgacgggtt aatttattgc gataattacc atttggcaat ttttcataat tagcataaat
      361 tttctgtaac gtttccgagc gtaaataagc tcggttatat agcccatcta ccggattatc
      421 tgtcgataaa tagcgtcccg tttcggcatc aatttgctga gacaaacggt taaaatgtgg
      481 atctaccccg gcttgatggt ttggcgtttc aaaacgattg agatcttttt gaactactgt
      541 caccactttc ccgtctttcg ggtctgtgaa ctgataagct ttatagccat caatattttt
      601 tacctgtttg gcatcattcg gtaaagcgtg ccaacaatca ttatgcaccc ataccccttc
      661 attatcggca tttgtacctc taataaagta agtatgatct ttctctaccg tcatattgta
      721 ggcttttaat ctttcctgtt tgatttttac cttagtgaca gtctgccact caccactttc
      781 agcaagtaag cgatagcctt tttgcaaatg ttgagcatca acccactgag cttttgctat
      841 ctctccgttg taagaatgcc cctccgaaga aatcggcact ctgcccgata atacttgcgt
      901 aaagaacggg tggattttgt ttgacacaat ggtgtggtat tttcctttgc tgtctgcgac
      961 ttctacatac accgtataat cgtatggatt actgtaatga gcttggactt tctgataggt
     1021 ggttataccg gtgcgttcat ttttcgccag caccttatcg cccactttaa tgctggaaat
     1081 ctgtcgataa cctttatcag ttttgacttc catatcaccg tggaatgagc aagtgcctaa
     1141 actagcgctc gatctggtct taggaatcgc aagttcttta cctgatttgg tgactatttt
     1201 cgccgttgtc cctacacctg ttttaacaag agcaccagca ccggttaatg ctgtcaccac
     1261 atcagtcatc atattgccaa actcttttcc ggcattaaaa gaacctacag cccctgcctg
     1321 ttcatagtaa acctgcattt tatcgagttt agctaaatac tcctgtttag tcatattagc
     1381 aatataggag agcgtatctt ctcttgcgat caattctttc atcgccacaa tagtttctat
     1441 tgggcttgag aacatggaga tcaatccttc aacgctctca gcgagattct caggaacacc
     1501 agcaacggcg cctgatacaa aactaacatc ttgcccaata tcaactaatc cccaatataa
     1561 tcgaacttgg ttttcacagc gggcatcacc atggcaggat tttaattctt gtgagcgttt
     1621 ctgctcctgc cagttcgaga ggtagttatt ctccactgcc cttttcccaa tatccgcccc
     1681 aaccgctgcc gattgtgtac tatcccctat caaacctgaa gccagtccgc ttgcaagctg
     1741 gcttagcaac gccacattct gtttctcggc ttcggttaag tcttgcgtgg atttgccctt
     1801 gtagagggtt ttaactataa agtccgctgc cgcttcgctc cctgcgcctg cagcagcccc
     1861 tgccagtaca ttgttccctg tcgcagccgc ttccaccgca cccagtaagg cgtgggcaat
     1921 caggttggct tgggtatccc cttcggtgta ctttttgatt tctgcattta agtatggtga
     1981 ggctaaaccc actgccgctt gtcccacgtc tttcgccgct aaaccttgca aggctgccgt
     2041 caccgcttcc accgctcgtt tggttttact gcccgtgccg tagtttttat caaactcttt
     2101 ttgcaagcgg tctaaattgg cttctaattt accaatctct tccttgctcg ctttttgagc
     2161 ttgtgctttg cccaattcca gcttcgcttc cgtcactttt tcacgctcac tatgcgtgta
     2221 aatctgataa gccttttctg caatctcgcc tatgcctttc accagctctt gtgtttcttt
     2281 cacctcactg atatcgtatt tcgtcacttt ttgattggcg ttttgcgtat ctcggttgag
     2341 atgattaagc gtagtttccg catttaggtc ttcggtgagg gtgatagcga tattttctcc
     2401 aatcgctgcc tttgttgtgc ttcgctcgct ttcgttttta ttgcccagta agctcagtgc
     2461 cgacatcggg ttcaccccac ttgtccctcc gctaatcgcg acacttcggg ttttcaattc
     2521 actgtggttt tcaatatcgc cggtggtcag cgtgccggct tggaagcggt tgttttcttt
     2581 ctccgcttgg ctagtcatta ccgctccctc aaaatgggcg ttctttttca ctttaacgtc
     2641 cattccactc ttgcctatgt cgattgcagt ttggttttcc acctgagcag tgttcaactt
     2701 cgctccatta tatgccacat gaccatttac tcctgttgtc cctaccgcca atgtgccatg
     2761 tacgctgcct tctgtgtatt cgcttttata tttgcttata tcttgtcgac ttcttgcctc
     2821 aaaatcttgt acctcacctt cccaatgatt tgctttgaca ttggttgcat ccagcatgag
     2881 tttaccattt gcagaatttg taacaatttt acccgcttgc agttggttgt tctgccacgt
     2941 ttcagtttcg atattctcct tgcctttacc tccactcact cctgcctcaa aaccaatacc
     3001 aaaactatct ctatctaaac cgataaatac gcccacactc gcactgtgat tcttattctt
     3061 agtgttgttt tgataagtat ccgcaacacc tttggattca atacccagct taccgtcaag
     3121 cagcagggtg tctttcactc gggtatccac cccttccagc gcaactttgc ccgctcccga
     3181 aatcaaggca atattgccgc cgttcagggt gcttttgctg tgggtcacgc tttgactacg
     3241 gctttctctt tgctgttttt gcgtgccgag actgactgaa atcttcaccg ccggattttc
     3301 tgccgcagca tggttttctg tgagtgcatt gccaagattg cccagcgttt ctgccacttt
     3361 ctgagcattt tgtgctgcaa tcacggcatc ctccgccgct tttaccgcat ataccccttt
     3421 gagtttctcg ttttgtgttg ccttcgcttt ttgcaccgat tgccgtgccg attgcaccgc
     3481 atccgttacc ggtgtactca acgcaatgct taagcccgac tggctaaatt cataattatc
     3541 ggtgctgtta ataacatctt tccctgattc aacccgtacc ccgtttttgc ctgaaatcac
     3601 ggcggttttt tcgttggaaa tcagcacatc gacattgctc acgctcgctt tattgccggc
     3661 ttgaatccga atattgcccc cttcactgcc caatgtattg cgtgccgtgc tttggattgc
     3721 cccctctaag ctgttctctt ggcgtgtttt ttggctaccg atagtaaagc cgatttcacc
     3781 accgttcaag aaaccgctgc gttttgtctc acgaatatgc tgttctgaaa gttggtttgt
     3841 tgccgaactg agcgttacat tattaccgcc gataatatca atatcgccac tgcgtgagac
     3901 ggcaagaaga ctttcacctt gaatatcgtg accggctttc actaaaatgt cttcaccggt
     3961 tagttcagtg cctttgtgtt cggtttcaga aatattgtca taagtcgttt ttgttttctt
     4021 cgctagcgca ccaccacttt ttgttttgtg atactcttca taatcgcgag aacgtgttgc
     4081 gctacccaac accatatcat tttcagccag taacgctaaa cgcgctttgg catccaaatc
     4141 cgctccttca ctgtaaaggt ttttccctga aaccaccaca tcgcctttgc cttgcacccg
     4201 tgaaatgacc acatcattca aggcttcatt acgatagtga ttgccaccgc ccattttttc
     4261 atcaaaacca acctgtaaag cggtcaaatt caactgattt tttgcagata agtaggtctg
     4321 cccctgtccg tcattgatga tgtctgcgcc tgctgaggtg atgttgcctg cctgtacttg
     4381 cagcagtcca ttttcatctt taacgtgcag tagggctttg cggtcaaggt tggtttcagc
     4441 gcgtttgaaa ccgtctaggt caatctgtgt ggttcgagtg gtggaggtat ggttaaaatt
     4501 gcccgcaata tccagcaaaa tcgctttatc cgcttcaatc ttgccgccga tattctctag
     4561 atcaccgctc actttggcag caagcacgcc accactgagt gtgccactat tctctaaccg
     4621 ttcggcatta aactgtgcta aacggcggcc tgctatcgtg ccttggttta caatctcttt
     4681 actgttgatg gtaagcgcat cggctgaaat caggctgcct ttgccgtcaa tatcaccttt
     4741 gcgtgcaagg acatacactt tcggcactaa taccgatacc gtttcaccgc ttggcaagcg
     4801 aaccgtttgg gtttcaaacc aaacaatatc gctggtcaag cttgccactt gctccggcga
     4861 taaggtaatg cctaaccgaa gattgaattt ctgcgcaaag gtgatgccgg catccatcag
     4921 ggctttatat tggctatcaa aatccgtata gtcgcccaaa aattgtctgc cggttaagcg
     4981 gttgatttgc tctcgaatta agcgctgttc ataaaaacca tcgcccaaac gtttatgcac
     5041 attgttatgg tcgtggcgta gggcattgaa catataatca ctgcttaacc agcgtttttt
     5101 atcggtgaaa tccggatcgg tttcgatcag aacatggcta tccgcttgag ggttaatgcg
     5161 ataaagccct tggtttggca aacgggtatc gaccgtcatt gagcgaacct caaatgcggc
     5221 tgcattcggg cttggtatgc gttcaatcgc cattgttggc ggattagggg catcgggtag
     5281 attaccccgt tcaacctgat tgccttccgg cttcacggca tttgctgctt tagcgtccac
     5341 ataatctgta taagcggtcg gtgaagtatt ttccagctcg acaaaaatat tcatatcctt
     5401 atggacttct tttaaacgag cgagaggacc atcattttca tcgtagcggg agtggcgacg
     5461 tcttgtgcct ccgtggtact cagtaaaaga gaattggcgc cttcccagtt cccaatcttg
     5521 gcgaacagca tctgcatcat caaggttggc aattctactg cgaccaatcg ggtttaacgc
     5581 tttgcctgca ataacccaac ttttatcgtt gataaattca tcgctttgat aagtgagatc
     5641 gccacctgcc aagatctgtg ccggtaagct ggctttggtg atattctctt ttaccatacg
     5701 ctctttaatc cacaaattcc agaaatgcgg gtattcttgg cttaaattcg ctttattgtg
     5761 ctttgcaatc gcttcatcta atgcttgaat attggcacca tattttgctt cccaatcaat
     5821 taacggtttt aatagcacat aacgctgata agccgcttct gccgcttcat aggctttcag
     5881 ttccgcttga tatgtttgta gtcgtttttg gtattgggct tccgtttccg tagagcctct
     5941 aacaggttca atcggtcttg ccggaaccac cggtgcaatg ccttgccctt gttccttctg
     6001 taatttttcc tgctctgcct cacttaaatg ctgattcgct aaatcttctt cattcattgc
     6061 ttgaataatt tcaggcgtca tttccggtgc atcttcagct ggtggtgtta aattaaacca
     6121 cgtccatact gggttatctt ttttataatt acctgcaggt aatagagtac aatctacact
     6181 ttcatcattc gcacagcttt gagcataggg ttttgccaat acggtcgtgg cattcagctg
     6241 gctaatatcg gtaattaccg gcaagtttgc ttcatcatac ttttgccaat agctccacgt
     6301 tttgcgatag tagcctttgc ggagttgatt cacattaatt gtcggcaaat gcccgttatc
     6361 atatacatca cttccacctc gattatgctt atctcttggc aaaataaaat gttcgttcac
     6421 agtttggcga tcagtggtct ccaattccga acgataatga ctatttacat tcgctacctt
     6481 atccgcattc aactgaatac gcccttcagc ttcaataata ctgctgcggt tatgtagctg
     6541 ccctgcgttg ccaataacgg tatcttgctc atttaattga cgtccaatcg ctatctctcc
     6601 tccgctatat attaatgaac cgcttttctt attcgcttca taaactaggg tattattttc
     6661 aattacttta gccgctaaac cgagagattg acgagcagca atagtggcag aggtcccgtc
     6721 tttatcttga tttagtatcc tctccgcacc taacgccacc ttatcgccat aaatattccc
     6781 tgtgccgata ttgtggattt cttgagcttt aatcacagtt tgagcgttat cgttgtccga
     6841 gcggctattg attaagcctt cgttatagac ggttgaagtt gcagtcaaac gggtatctgc
     6901 cgagcttatt cgggcatttt gtgcattggt tatttggttt gccgtgagct gcaagcggtc
     6961 atttgcggct aattttttgc gattgagaat atcgccctca gtctgaataa tcatcgacct
     7021 attcgcattg atatctttgg taatatcaaa gttctcttgc aaattcaggc gaatatcgcc
     7081 tgcttgtaca tcaccatcac cggataacgc tttggcagtg atctccaaac cattatttgc
     7141 agaaactttt ccctcggtat tcgtcattgt gagtgatttt ccgccgaaaa tagccagatc
     7201 gccccaactt aaaatttccc cattacggtt atttaagctg tcgctgaggg taaatcgtgc
     7261 tgatgagccg atataaatac cgcctttttc attattgagc tgttttgtgc tgttcagtac
     7321 aaactgcgat gcaataatcc cctgtattgg ctgaggttgg cgtgccaagg taccttgatt
     7381 ttccacctcg aaactgttca tcgtaagtga gccacctgct tcaataagtg agccattttc
     7441 cgagatccga ttatttaaga cagtcggtac attgagcgtg atatctgtgg ccgatttcag
     7501 taccccacct tggttatcca aaagcgtgct atttagggtg agatttccca cactttgaat
     7561 aacaccgtgc cgattatcta cttgttggga aaccgtgagc gctaactctt ttactgaact
     7621 aatttctccc tgctcacggt tatctaagga ttgagcgtta atcgcaacac cgctggcgga
     7681 aattcgcccg ccttgatttt gcagttcgct agttatatca agggtttgct ccgtttgaga
     7741 ggtgatcacc cccgattgat tttttaacgt attgaccgta atcgcctgtt tttcgccact
     7801 caaaatcaga ccttgctgat tattcagcaa tggagcacga atagataact cgccttttgc
     7861 aaccaatttg ccatcgactg aatccacagc atcatttaaa tttagcccta aacgcttgcc
     7921 actacttaat agcccttggt tatttgttaa acgattaccg tcaatctcaa aatgtccacc
     7981 cactgaaaag cgagccgagt cagtatggat actctctgct tttaggcgaa aatcctgctc
     8041 accagtctgc ttccaaaccg agccacgagc atttaaattc gcttgttggg cctcaatcac
     8101 ctctgcggtt agctggcttc cttcagtgga aagccatttt ggggtggtga gcttgatctg
     8161 atttgcacta aactgcgcac tattcgcctg aatatcgccc gattttgcac tgtattcaac
     8221 ggtatgggca ttattttggc tctgattggc atcaatctca gctgccgcca cgttaatttt
     8281 ccccgaagcg atattttttc cctgcacagt ggttttttct ggggcagtga gttgaatagt
     8341 tttccctgcc acagattgag cttccagctt ccgtacttca ccttgtgcag tgtcggttac
     8401 gtccacccca gcggcaatca gtgagttttt gtcggcgtga atttggcggg cggtgtaggt
     8461 gatattgcct tttgcgaccg tttcgccact ttgctggatt ttagtttcgg cttttttctg
     8521 aatattgcca cccctcgcca cgatggaacc gctattttga atatcggctt tcgagttaag
     8581 ctgaatattg ccctgtttat tttcaatttt gccttggttg tcaatcccgt gttgggcatt
     8641 gagttgcact tgtctttgag cgttgaacgt gccgctgttt acaatccgcc cttgactgtc
     8701 aatggtgagc gtatcggcac ttgcaccgat atgcccagcg ttacgcacgc ccacgccctg
     8761 ttccgtacca atcaggtgga ttttgccggc atacattccg cccagctcac ccacatcaat
     8821 cgcaactgtg ggcttttctt cccctgcaag cggttgattt gtatgaataa tttgcaaatt
     8881 gttgtccgaa tttgaccgct tgacggtgtt tttgccggta atgactttag tctctttttt
     8941 cgaccagaca ccggcgttga ttttcgcttc tcgggcgagg atatcggtgt aatccactcg
     9001 gctgttgtcc agccctttgc cagcaaccgt taccttgcct ttttccacta caaaaccttc
     9061 taaattgcct tgatttatct gtggttttcc ggtggtcagc gtggctcggt cggcgttgat
     9121 aataccgcag ccgtcacagt gcaagccgct cgggttggcg ataatcactt ccgctttttt
     9181 gccagccact tccacataac ctttcattac cgaaggctta ctggaattga cttcatttaa
     9241 aatcaccttc gcttcgccac gggcaagata cgggttgcct tgcaccatac cggcttgttg
     9301 ggtggcggtg ttggtgcggc tgttgttgag aattgccccc ttctccgcta cgtcaaattg
     9361 gctgtattta ttgtgggaaa gccctttatc gttcggggtt tgaatattga tttgtggcag
     9421 cccgttggcg gtttgcaaaa taatcggttg ctgatttttc ggggcagtgc tatcggcttg
     9481 aataatcaaa gtttccgcca tagcgttgtc tgataaggta acgaatccca acgcacagaa
     9541 caagctgaac gcaagcggtc gaattttgca aattttttgt aaaattgcac cgcttgtcat
     9601 actttcactt tctgatttac ctgctaattt tgctaattca gataccacca caatacgttg
     9661 taaggtcttg ctgaaaatag tgcgaaaaca atgcttattc ataataattc cttaatagca
     9721 caatatactt aatacaatat gcccaatcat tcattaaaaa ttgtaaccaa tattaaaacc
     9781 ggcaacggtg tctgaggttt taaacccttc cggttttcgc atcggtctac cggcaaacac
     9841 gtcataacta aatcctttaa ctccaccccg aaacccaatg gctgttccca ttaaatgatg
     9901 tccgagctga ttttccgttg cccaaccgga cactcgacca ccatctaccg cccaataaaa
     9961 agcgtggttc ggattaaatt gccagctcaa ctcattacgc cagttccagc cccgctcacc
    10021 ggaaagagtc agttcaccgt caaatccacg tatggtgtaa cgcccgccaa tcgaaaaacg
    10081 gtcttgcgga accagagggg ttttattcca ttgggcattt aggctacttt gccactgcca
    10141 tggctgcttg ccccaaaaaa acggtttcgt caggcttgca cttgccgtga tgattttagg
    10201 gcgagaagtc ccttcgccat aatcttcctc gggagcagat aatgcccctc gtgctgccgt
    10261 acctcgtttg aaattagccg aaagctcaag cgtagcggtc gggaaatatt ctttatgcga
    10321 aaaacccgct tcccaacctg ccattcggcg tttttgtact tcaatttctg agccgtcaat
    10381 atagttttgc gactggcgtg gccaaagtga accggacaag ctcgttttac gtttggcatc
    10441 acgataaagc aaataagaaa aggttaattt atcggtatca ctttcaccgg cgtagagata
    10501 gctcttgtca aaggcaccaa atacttcttg atgataacga tgatgagtgt gtgaaaatgc
    10561 taactgccaa tagccaaacg gcacggaata gtaaagcgtg agatttttac tggcacgtct
    10621 gccgttatca tcaccttttt gtttcatcga atgggtaaaa gaggtataga acaggtcatt
    10681 tgccgaaaaa atattatcta tagacagagt ggcggaggtt tgtaatttgc ctgtggattt
    10741 cgagccggca tcgtccagcc caagatttaa gcgaaatgga aacgcttgcg aatagctgat
    10801 ttttaaatca ctgtctccca cctccgcttt ttcgcttgag ctgggtaaaa tctcaatatt
    10861 tgcctccgcc gtcggcaccc gttttagatt ttccagagac tgctcaatat ctcgcacatt
    10921 cagaatatcg ccttcggcaa aagtaaaacc ggttaaggcg tgcagcttgg taaaacgcgg
    10981 tactttgccg ctatctacca ctaaagtatt gcgtattttg cccggaatca cggttaaaac
    11041 taattttccg ccacgtaaat cttgctccgc agtaaccact cgtgtagtga cataaccttt
    11101 gccaataatt tcattttgga tttgcttcat caacacagct aacccttcac caccaaagca
    11161 gtgaggtaat gtcaaatgaa atgattttac tgctttttca aacgcccatt ggaattggct
    11221 ggtagtcaga gaattgtcag gagaatagtc aatgagagtg atttgatgaa tggggtaaca
    11281 aggcaattcg tgattggaaa gagtaagtgt atcattgatt tgtgtctcga gccgtacatc
    11341 agctttcggc tggaattttt ctgcttgatt ttgttggtat tgctgttggc gatgttgctg
    11401 ggcagcatca atttgttgtt gagttgcatc aatgcgtgct tgttgtacaa ggcttgaaga
    11461 atttgcttca gcaacaaaag aagcacttaa caataccgtg cttaaaatca gattttgtgg
    11521 ggttttcata agatcaaata agtaagacat tcaacgtttt tagtctataa aaatttatgt
    11581 tgttttgcaa actccgtttt atggagcttt atacatatct gacgactttg gttttttatg
    11641 ctattatctc cgccattttt atactataga gaatagaata accgtgttag tttcagattt
    11701 tcatttcaac ttacccgatg agctgattgc tcgctacccg actgccgaac gctctgccag
    11761 ccgtttatta cacttaaatg gcgaaacagg cgaatttgcc gatcaacaat ttgtggattt
    11821 atacgatcat atcaacgaag gcgatctatt gatcttcaat aacactcgag tgatcccggc
    11881 tcgcctttac ggtcgaaaac ctagtggcgg caaagtagag gcattagtgg aacgggtgct
    11941 agatgaacac cgctgcttgg ctcacgttaa agcctcaaaa gcaccgaaag aaggggctga
    12001 attgatcttt ggggaagata agctaggtga aggcaaaggc tttacggcga caatggtagc
    12061 tcgccacgat actctgtttg aattggtgtt taacgaagag caaccgttat tcgaattgct
    12121 acagcaagct ggtcatatgc ctcttccacc ttatatcgac cgccccgatg aagatgctga
    12181 ccaagagcgt tatcaaaccg tttatagcaa agtgttaggt gcagttgccg cccctaccgc
    12241 cggtttgcat tttgacaatg caatgcttga aaaactaaaa gcaaaagggg tggatactgc
    12301 cttcgtaaca ttgcacgttg gtgccggcac tttccagccg gtacgggtag aaaccattga
    12361 agatcataaa atgcacgccg aatatgccga agtgagccaa gaagtagtcg ataaaattct
    12421 cgcaacgaaa gccaaaggaa aaagagtgat tgcaattggt acgacatcag tacgttcggt
    12481 tgaaagtgcc gcccaagcgg ctgaaaaaga tgggaaattg attgctccgt tcttttctga
    12541 caccgctatt ttcctttacc cgggaaaaac ctttaaagtc attgatgcgt tagtcaccaa
    12601 tttccattta ccggaatcca cgctaattat gttagtctca gcatttgccg gctatagaaa
    12661 tacgatgaat gcctacgccc acgcagtaga aactaaatac cgttttttca gctatggcga
    12721 tgcgatgttt atcaataaaa acccaaatgc gttgagtgat gttccttaat ttatgagggt
    12781 tataagggaa taacattatg aacaaaaaca atcttccaac cattttaagg gattgttttc
    12841 aatatattca ataatttgga agtacgatgt ttcattacga ataatgtgtt cataataatt
    12901 cctttgccaa atttcagaaa tatcggtatt ttgacgaacc catttagtta cgccaatttt
    12961 aaaaccacgc acaatagagc cgattgttcg tgatgttccc cgtaagggcg aaacattttt
    13021 cgcccttata catggggcaa atgatgattt gcccctacag tttcgacgat ttcaataatg
    13081 ccatgtaaat gattaggcat aatcacaaag ctatgcaagc ttacatttgg aaaatgatcg
    13141 ggtatatctt cccaacattg acgtacaata ttccctatct tattaagcct catttcatca
    13201 tcaacaatct caccgagaaa atggtgttta tttttacaac aaatagtaat gaaatagaat
    13261 ccttgtgatc ggtaatcata atgctttaga cgaattgaac gcctcctatg aatctccggc
    13321 tgatatttct gcatttttct tattccttaa taaaactgct tataccaagg ggaaaatgcc
    13381 ccccttgcct gctcaatttc aatagaactt gggtaaaatt ttactcttat cattcccgtt
    13441 tctacggtat ttaatacatt aatatgtctt ctgtttaatc tctcaataat ctgtttattc
    13501 ggtaatttcc atgggttcca tcttccagca gaaatcactg caaatgctgg ctgggtaatc
    13561 gataataaag tttcgccagt gctgcttttg ctaccgtgat gtccaacctg tagaaaatca
    13621 attttgccga gctgatgggc aaattggcgt tcttgttcca cgccagaatc ccctgtcagt
    13681 aatatcttca agcggtcgtt tttggctaaa ataacgcaag agtcttggtt tttagctcgt
    13741 tctgcaatgt gtgacgggta aattgccaat aattgccatt cgccaaattg ccacgatgtt
    13801 ccggcaatac aaggctcggg atttatattg tgatacgtca ccttgcttga actaattaac
    13861 cttgcttttg ggtatgcttg caataactcc actaccccac ctgaatgatc attatcatcg
    13921 tgacttagaa aaatcgcctc tacctcaatg ccatttcgct ttaaatacgg caagatttcc
    13981 atttttgcca ttgaattgcg actaccatca tttgcttgcc aactggagcc ggtatcgtaa
    14041 attaccgctt tattttgttg ataaatgaac gccattgcca gcccttgccc aacatcaaac
    14101 ataatccatt ctgtttgggt ttgattaaag agcttaggga gataaaaact cagattgaac
    14161 aatagggata ccgctactgc acttaaccac tttttataag atagccgata taatttgcaa
    14221 tacagcaata ctaaaaccaa taaattcagt gaaagtaact gccactgttg ccaatggctt
    14281 aatgcaaacc acgcattaga aaatggatct aacagccata ggctaaattg agcaagatag
    14341 tccgccaaca gccacgttga aaacagattg tcggtcaaga gcgtaaataa aataagcggt
    14401 acaagcaaaa agctatacaa cggcacaata atcaggttgg caagcagtgc aaaagcagag
    14461 atgccctcaa aaaagtagag ttgaaccggt gagaaaatca gccaaatgcc gatttgtaag
    14521 tgcaaaagcg ataaccacaa gcggttaaat tttgtgattt ttttacgata ttcatcgttt
    14581 aaaaatcggc taagaggaaa atattgatac cataaaatta aacttgctac cgctaaaatt
    14641 gagagccaaa aactatcaga aagcaggctc aatgggtcaa ttaaaatcag taacgccact
    14701 attctccccc aatattgcca aggggtatag tgtcgccgca gaacttggca aagcaagaca
    14761 aacgcaatcg ccagcaacgc tctggcggtc ggcacggcaa aaccggctaa atagctgtaa
    14821 ccaaatgcgg tgatgaagct aactattctt gcaaaataaa gtgaaaatcc gaccgcttgt
    14881 aatgcggtaa gctcgctttt caagcaaagc cattgcacgc ctttggcaaa gtaaacacca
    14941 aacaacattg ccagcccgat atgcaagcct gaaatggcaa ttagatgggc agtagcggtt
    15001 tgttggaatt gcccccagtg ttctgttttc agccacgccc tttcaccaaa agccagagcc
    15061 aataaaaagc cttgcgtagg caaagcgtcc gtttcagctt tcactttatt cagccaattt
    15121 gttcgcaagc tataatcagt tttggctaat gtttccacct tgcgaaccgt tgctgtcata
    15181 ttgatatgat tggcaaaata ccaacgctgt cggtcaaaat taccgatatt gctacgagct
    15241 gaaataggac gaagagccag ttcggcttga tagaattgat taagtaacaa cggctgtttc
    15301 gattgccagt tcaagtagat cttctcgcct gtatttagtg tcgcaatcgc tgtttgataa
    15361 tcggtttgtt ttaagatttt actgatttca aattgaacag gcgttttact agcagtcgtt
    15421 ttttccgcat tttttgcaac ttgaatgatc tgaatatagc caatacaaac caataagccg
    15481 agtacagccg ttaattttga gcgtagataa acactcaata tcagcactaa ggttcctatt
    15541 actataccca caaatagaaa cgctttaggc aaccataaaa taggcagatg aaatagaatc
    15601 agggcaatac aaaaacggtc taaagtcata attcggctta ctttttaaag ctgagtatgc
    15661 cgtttgtgct taatattttc agaataaatt ttcagatttt cgacacagtt cgcaaaaata
    15721 cttgaaaaaa taaaggcaga ctttttagcc tgccttataa agaagagata ttatgctgat
    15781 ttttgatctt cagcgtaact tactgctgca gtgaataata cgtctgttga tgaatttaat
    15841 gcggtttcaa ccgagtcttg aatcacacca atcacaaaac ccaccccaat tacttgagca
    15901 gcaatatcgt ttggaatatt aaataaacta caggctaatg gaatcaataa taatgaaccg
    15961 cctgccacac ctgatgcgcc acaagcacag attgatgcaa ctacgcttaa tagtagtgct
    16021 gttgcgaaat caggttgaat accttgtgta taagctgccg ctagggttaa tacggttaca
    16081 gtaatcgctg cacctgccat attaatactt gcacctaatg gaatagctac agaagcgatc
    16141 tcatcacgaa cgcccaaacg cttggctaaa ttcatattca ccggaatgtt ggctgctgaa
    16201 ctgcgggtaa agaatgccgt tacaccactc tctcgtaaac aggtaaatgt aagtgggtat
    16261 gggttgcggc gaattttcca atacactaat aatggattta gtactaatgc cacaaataac
    16321 attgcgccaa ctaagactac taataaacgt gcgtaatctt caaacgcatc aataccattg
    16381 atggcaacag tttcagctac taaaccaaat accccaatag gcgcaaatgc aatcacaact
    16441 ttcacaacaa acgaaactgc gttagctaaa tcatttaaga atgcttttga acttgccgaa
    16501 ccatggcgga atgcaatccc taaaccgata gaccacgcaa gaataccgat aaagtttgca
    16561 ttacttaatg cgactaatgg attatcaact aagttaaaaa taacggtttt tagaatttca
    16621 ccaatgcctt gtggcggggt taaaccgtct gggcttgcag taagctctaa tgtagttggg
    16681 aacataaagc ttaaaacaac agccgttaat gccgcaaaaa atgtacctaa tacataaagc
    16741 accagaatcg gctttaattt gctatctgaa ccaacttctt tatttgcaat cgcagccaaa
    16801 actaatacaa atactaaaat aggcgcaatg gcacgtagtg atttcacaaa aaattgacct
    16861 aatacaccaa cgctggtagc ccattcttta ttaataaatg ccagcaatac ccctagcact
    16921 aaaccaatgg cgatacgtaa tactaaatta ccgccaagaa tttttgatga taatgattga
    16981 ctcataaccc
//
LOCUS       DANLXS010000034        16755 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.034, whole genome shotgun sequence.
ACCESSION   DANLXS010000034 DANLXS010000000
VERSION     DANLXS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16755
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.034"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..334)
                     /locus_tag="PXD71_000851"
     CDS             complement(<1..334)
                     /locus_tag="PXD71_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6290998.1"
                     /translation="MPFYAGQVKCIFIDPPYNTKSAFTHYDDNLEHSIWLSMMYPRLE
                     ILRELLAEDGSIWITLDDNESHYLKIMLDEIFGRKNFISNLIWEKKRKPSFLGKVGKV
                     TDNLFCYAK"
     gene            complement(766..3252)
                     /gene="torA"
                     /locus_tag="PXD71_000852"
     CDS             complement(766..3252)
                     /gene="torA"
                     /locus_tag="PXD71_000852"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL6290999.1"
                     /translation="MKKQDNIDVSRRGFLKNSSLSLAAGAGLASGTTGVVGALASTSA
                     KAAEMKTVVTAAHWGPLGVVVENGKVVKSDSVFEISVPNELQHVVADQVHGETRVKYP
                     MVRKGYLEGNKDTTLRGRDEWVRVSWDKAFELVANEMKRVREAHGANAIFGGSYGWYS
                     SGALHASRTLLQRYLNVTGGFVGVKGDYSTGAAQVIMPHVLGTIEVYEQQTSWEVILE
                     SSDIVVLWSANPLNTLRIAWTSTDQQGIEYFKKLKESGKRIICIDPVKSESCEFLGAE
                     WVPVNTGTDVALMLGMAHTLVAEGKHDQAFLKKYTTGFEKFEEYLLGKTDNQPKDAKW
                     ASQICGVPEETIKKLATDFQSKRTMLMAGWGIQRQRHGEQGHWMLVTLAAMLGQIGLP
                     GGGFGLSYHYSNGGVPSATGGILGSITANPAVEAGAKTWLDETSKSSFPVARISDALL
                     NPGKTIDYNGTQITYPDIKIVYWAGGNPFTHHQDTNTLVKAWQKPDTIIVNECHWTPT
                     ARMSDIVLPATTSYERNDLTMSGDYSMMNIFPMKQVVEPQFEAKNDYDIFAELAKRAG
                     KEAEFTEGKSEMDWLQSFYQTAFEAARKNRVIMPKFEKFWQENKPLSFKASDKAKKWV
                     RYEEFRNDPLLNPLGTPSGKIEIFSEVIAKMNYDDCKGHPTWMEHEEYSGKTTAEEPL
                     ALVTPHSKWRLHSQLAYSSLRKQYAVNDREPVLIHTEDAAARGITSGDIVRIFNKRGQ
                     VLAGAVVTDGIIKGTLALHEGAWYDPLDLGTSEQPLCKNGNPNVLTRDEGTSKLAQGN
                     SPNTATVQVEKFTGQAPEVTVFKEPKYTQG"
     regulatory      complement(3271..3467)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(3471..4586)
                     /locus_tag="PXD71_000853"
     CDS             complement(3471..4586)
                     /locus_tag="PXD71_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL6291000.1"
                     /translation="MAKMKKIAVFVGIMALGAAVLGGTQYAMKATSSTEFCVSCHSMT
                     HPQAEWEGSVHFSNRKGIRAECSDCHLPQDGLHYVKAKIIALKDVWHTVVTNKLPDQE
                     AYEAHRLEMAQRVWAEMKENDSMTCKSCHSFDAMVLSEQTEAAQKMHKLAQETNQTCI
                     DCHKGIAHFMPDVPVDNAAAMGELAKHGAEFSTNDKTLYTLAMTPVQLAQGGEIRLLP
                     YAELHQWTAEGEQVKATVNGWQQEGAESVVYMDLGKRIMVALLPDDAQSKVNVTKTVF
                     DNVTNSNWKEINFEITAPKNAVTANITALNHFGDNLNQTHCSGCHAPIGAEHYTANQW
                     IGVVNSMKDRTSMSADDVRAVTIYLQRNAKDVAGSSH"
     gene            complement(4728..6479)
                     /locus_tag="PXD71_000854"
     CDS             complement(4728..6479)
                     /locus_tag="PXD71_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6291001.1"
                     /translation="MDKRSRWSCLVNEQGEISEWEDKAGADYFAELPQICHYAQALLY
                     SDKNSLQFYYSSNHQWLKISLERHQSFVLTQAEHTDLPYGLSKREIELLTLISSGLSN
                     EDIANELCISKRTVDKHIENIFAKTHTKSRTLLAVFAITEHLICLPTPGKLTQSILAS
                     FNIEQLALQIIAKKQPLVTASTRHEPPIFTINHHHSRPIIIGVPYVEQGIGQIDTREL
                     LNGTQLAVETINQQGGIHGREVQVATAGFYVKDKASILQAYHHLFDQEVDAISTAYAC
                     YLPEVHELIATKGIPYLHIATHSGSNKLAQNLPNQRIENMFQVCASDVTYGLGISHFL
                     QYYRYHYAPWLQNKTVMVVTVSWQKVDIGIESLTLSLREQGWNVDYLELTQEQDNYAT
                     LMAQVHNLAPSIIVFASYFAEDIVQFYQTFIRNPTNAIIYSIYAPSAFLPHEKLCEGV
                     LWSTNSGLSENYLGRQFSQHYERFFGKSPTYSQASIAYDQANILANAWKQSVSPRHFK
                     AASNAIRLQPHYGVNGTYYFNTDSQIGLTYAETHDLSISLPQLVYQIQQGQSRVIAPE
                     LFANAQFILPPWFSEKA"
     regulatory      6643..6799
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            6788..7813
                     /gene="moaA"
                     /locus_tag="PXD71_000855"
     CDS             6788..7813
                     /gene="moaA"
                     /locus_tag="PXD71_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDL6291002.1"
                     /translation="MQSIIPIKNVGDDVVSGLVDRFQRQYTYLRLSITDVCNFRCNYC
                     LPDGYKPPSHKQTFLSVDEIQRVAKAFANLGTEKIRITGGEPTLRKDFLEIAHTISQT
                     PGIRKVALTTNGYRLERDVELWQQAGITDINVSVDSLDPRQFQLITGENKLQSILKGI
                     DKAFEIGYPKIKVNAVLMKQYTAPELDKFLTWIKDKPIQMRFIELMETGEMDSFFQAQ
                     HLSGQSVMTRLLQEGWQLQPKAMSDGPAKVLSHPDFKGEIGLIMPYEKNFCASCNRLR
                     VSALGKLHLCLFGEEGIDIRDLLASDEQQLQLETRLKSSLQGKREHHYLHIGDSGVRN
                     NLASIGG"
     gene            8314..8790
                     /gene="moaC"
                     /locus_tag="PXD71_000856"
     CDS             8314..8790
                     /gene="moaC"
                     /locus_tag="PXD71_000856"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDL6291003.1"
                     /translation="MTTFTHINQNGEANMVDVSMKQETVRIARAEAFVRMNAETLQMI
                     VSGNHHKGDVFATARIAGIQAAKRTWELIPLCHPLLLSKVEVQLEALPESNQVRIESL
                     CKLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMIIENVRLLSKSGGKSGDFKVD"
     gene            8783..9028
                     /gene="moaD"
                     /locus_tag="PXD71_000857"
     CDS             8783..9028
                     /gene="moaD"
                     /locus_tag="PXD71_000857"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDL6291004.1"
                     /translation="MIKILFFAQVRELVATDRLDVEPTFTTAEALREHLSQQGKKWQL
                     ALEKGKLLVAINQTICPLETEIKDGDEVAFFPPVTGG"
     gene            9030..9485
                     /gene="moaE"
                     /locus_tag="PXD71_000858"
     CDS             9030..9485
                     /gene="moaE"
                     /locus_tag="PXD71_000858"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDL6291005.1"
                     /translation="MFETLIEVQEAEFDQNRIYQWLSEQHSVGAATLFIGKVREMNLG
                     DNVSGLFLEHYPAMTKKALQEIVAQARTRWDLQRVAVIHRIGQLHTGDEIVLVGVSSA
                     HRGDAYHANEFIMDYLKTKAPFWKREQTQAGERWIEGRESDQQAAKKWQ"
     gene            9518..10000
                     /gene="ybeY"
                     /locus_tag="PXD71_000859"
     CDS             9518..10000
                     /gene="ybeY"
                     /locus_tag="PXD71_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL6291006.1"
                     /translation="MENLYIDLQIACENTENLPSEQQFYTWVQKALAVEAKTDDFPES
                     EITIRIVDEAESHELNLTYRGKDKPTNVLSFPFEAPEGIEMPLLGDLVICRQVMEKEA
                     EEQGISAESHWAHLAIHGTLHLLGYDHIEEDEAVEMESLETEIMQSLGYEDPYLSEKI
                     "
     gene            10078..12096
                     /locus_tag="PXD71_000860"
     CDS             10078..12096
                     /locus_tag="PXD71_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HDL6291007.1"
                     /translation="MRTIAIITSPNNADFIQQGYFYIPENHQLKAIPFSNAKTILGQE
                     YPFAIYTMQAENGVNFNLDAFAILAGTIQENGTLFLLCPQWDNLENELDFDALRWNEN
                     QAIACPNFYQHFKQLVAKFGFEVRTNLPKLPATSGQIPSKIYQLTQEQQNILQNLPLD
                     PADIHLITAPRGRGKSTLAGRLAKALPAQNKVLITARSYSALPSFWRSAEQKIPFFAP
                     DNLIEQVKSKNISPNQWLFIDEAASLPLPILHTLCAYFEKVVLTTTTQNYEGTGRGFE
                     LKFPVMLDKPFKHWTLSQPLRWSENDPLEAFVTELLLLSEEKSPLFSKEGKGEIYSLR
                     SHYVPLLKQRSNAKRLWQKQLSKNISYSNKHLQEKIAFYHLLANAHYKTTPTDLRRLF
                     DGENQLLFSQQENEQLIGGIWAMQEGGLDEKLTQAIWLGERRPQGSLVAQYLCFQGNL
                     PQACKLHSIRISRIAVLPEKQNQGVGKRLISQFILQNSVEKRPLDFISVSFGLTTPLH
                     AFWQSCGFQLVQITPNKEASSGYQSAMMIYPISEQGKLFAQQATQAFARDFALQPFFT
                     EICEELQNFEQFRPLATHNLDERDWKNLTAFAHGKRTFSAAYVSLKRLYLSDKARFSE
                     LDIFERKGQKISKMEVENYRKLVLQNLNNKSFKIIYRFYYIFLCFCSC"
     gene            12161..13351
                     /locus_tag="PXD71_000861"
     CDS             12161..13351
                     /locus_tag="PXD71_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HDL6291008.1"
                     /translation="MFSHIQAAPADSILGLGEAFKADSREGKINLGIGVYMTDEGKTP
                     IVKAVKEAEKRLLETENSKNYLTIDGVQAFNAATQALLFGEGAEVITSGRAKTAQSLG
                     GTGALRIAAEFIKRHTNAKNVWISTPTWPNHNGIFDAVGVNVKGYRYYNKETNGLDWE
                     NLIADLSQAEAGDVVLLHGCCHNPTGIDPTPAQWEQLAALSAEKGWLPLFDFAYQGFA
                     NGLEEDAYGLRAFVKNNRELLVASSFSKNFGLYNERVGAFTLIADNADDANRAFTQIK
                     SIIRVLYSNPSAHGASAVAVALADPDLKAQWIAELDEMRNRIKEMRSQLVQKLKEKGA
                     NKDFSFITEQNGMFSFSGLTPEQVDKLKDEFAIYAVRSGRINVAGITSKNIDALADAI
                     VKVL"
     gene            complement(13458..14759)
                     /gene="folC"
                     /locus_tag="PXD71_000862"
     CDS             complement(13458..14759)
                     /gene="folC"
                     /locus_tag="PXD71_000862"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL6291009.1"
                     /translation="MNTLSIPKATDSLETWLSYLEQAHSKPIDMGLDRIRKVTTELDL
                     LKPAPYVITVAGTNGKGSTCRLLEVTLLKAGFRVGVYSSPHLIHYNERVRINGEVLPD
                     ADHVQAFAKINAKKTASLTYFEFSTLSAFELFKQNQLDIVILEVGLGGRLDATNIIDP
                     SLAVIISIDIDHVAFLGDNREDIGREKAGIFRENIPVVIGEPDCPHSIKQIADSLNCH
                     AFYRGKDWQFEIKNNLFHWYSEEKSFTDLPIPLIPIPNAGTALAVLMQLPFEIDEKII
                     RQALNEAQMTGRFQTLTDTDFTHFSQPKPAAKVIIDVGHNPHAARYLADKLRELKQPN
                     QKIYAVFSALEDKDLSGIVEPLANIIDEWHCAGLDCWRGQTGEAVLAKLVNVLPNSTA
                     CSYENVAQASRVLFETANENDIVLVFGSFHTVADFMLWLET"
     gene            complement(14769..15665)
                     /gene="accD"
                     /locus_tag="PXD71_000863"
     CDS             complement(14769..15665)
                     /gene="accD"
                     /locus_tag="PXD71_000863"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDL6291010.1"
                     /translation="MSWLDRILGRDSSSSSNAKSKVPEGVWTKCTSCEQVLYSEELQR
                     NQQVCPKCDHHMRIDARTRLLNLLDKDSAVEIAADLEPEDVLKFKDLKKYKDRLTAAQ
                     KQTGEKDSFIVLAGTLHNMPVVTASFNFEFMGGSMGSVVGAKFVKAAEKALAEKIPFI
                     CFSASGGARMQEALFSLMQMAKTSAILAKMKEQGVPFISVLTDPTLGGVSASLAMLGD
                     VNIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLEHGAVDMIVHRKEMRDTLSRL
                     CAKLTNQPTPFKVAELVVENAE"
     gene            15974..16755
                     /gene="mazG"
                     /locus_tag="PXD71_000864"
                     /pseudo
     CDS             15974..16755
                     /gene="mazG"
                     /locus_tag="PXD71_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600778.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
BASE COUNT         4883 a         3513 c         3484 g         4875 t
ORIGIN      
        1 tttttgcata gcaaaataaa ttatctgtaa ctttaccaac tttgcctaaa aaagatggtt
       61 tccttttctt ttcccaaatt aagttagaaa tgaaattctt ccgcccaaaa atttcatcaa
      121 gcataatttt caaatagtga ctttcgttat catcaagagt gatccaaatt gaaccatctt
      181 cggcaagtaa ctctcgcaga atttcaaggc gtggatacat cattgaaagc caaatggaat
      241 gctctaaatt atcatcgtag tgggtaaagg ccgattttgt gttataggga ggatcaataa
      301 aaatacattt gacttgcccc gcataaaagg gcaaaagaga ttttaaggct gataaattat
      361 caccctgaat aattaaattt tgcttgactt tagataaggt ttcagcagaa gctgagctgt
      421 cggctgtcgg ctgtcggctg tcggctgtcg gctgtcggct gtcggctgtc ggctgtcggc
      481 tgtcggctgt cggctgtcgg ctgtcggctg tcggctgtcg ggcaatatta tgcgagaagt
      541 ccggcaactc acgcaatagg caatatgggg tttgttttgc ttttttgacg gcttcttctt
      601 tgccgtacca agtaagggtt gtcataaaat gcacctgaaa agtaaaagtg gattattgta
      661 acaagcggtc aaaaaaatag aaagttttac aaaaaattaa gcaaatttaa ccgcttgcaa
      721 catagaaaag gcacagtttt atactgtgcc tttggttata tgaaattagc cttgtgtgta
      781 tttaggctct ttaaatactg ttacttctgg tgcttgtccg gtgaattttt caacctgtac
      841 cgtagcggta tttggtgagt taccctgtgc taattttgat gtgccttcat cacgggttag
      901 cacgttaggg ttgccgtttt tacatagcgg ttgctcgctg gtgccgagat cgagcggatc
      961 gtaccaagct ccttcgtgca aggctaatgt acctttgata atgccatctg tcaccaccgc
     1021 accggctaat acttgaccac gtttgttaaa aatacgtaca atatcaccgc ttgtaatgcc
     1081 acgagcggcg gcatcttcag tatggatcaa caccggctca cggtcattaa ccgcatattg
     1141 tttgcgtaat gatgagtaag ctaactggct gtgtaaacgc catttagagt gtggagtcac
     1201 caatgccaac ggttcttctg cggtggtttt acccgagtat tcttcgtgtt ccatccaagt
     1261 tgggtgacct ttacagtcat cataattcat tttggcaatc acctcagaga aaatctcaat
     1321 tttgccggac ggtgtgccta atggatttag gagtgggtca ttacgaaatt cttcataacg
     1381 aacccatttt ttcgctttgt ctgatgcttt aaaagaaagt ggtttattct cttgccagaa
     1441 tttctcgaat ttcggcataa tcacacgatt tttgcgtgct gcctcaaatg cagtttgata
     1501 gaaactttgt agccaatcca tttcagattt gccttcggta aattccgctt ctttgcctgc
     1561 acgtttggct agttcggcaa aaatatcgta gtcgtttttt gcctcaaatt gcggttcgac
     1621 cacctgtttc atcgggaaaa tattcatcat tgaataatcg cccgacatag ttaagtcatt
     1681 acgctcatag ctggtggttg ccggcagcac gatgtcagac atacgagccg ttggtgtcca
     1741 atggcattca ttgacgataa tagtatccgg tttttgccac gctttcacca aagtgttggt
     1801 gtcttggtgg tgggtaaacg gattaccccc tgcccaatag acaattttga tatcaggata
     1861 agtaatttgt gtgccattgt aatcaatggt tttgcccggg ttgagcagtg catcggaaat
     1921 acgagccacc gggaatgaag attttgatgt ttcatctaac caggttttcg ctccggcttc
     1981 tactgccgga tttgctgtga tagagcctaa aatgccccct gtggcactcg gcacgccgcc
     2041 gtttgagtaa tggtagctta aaccgaaacc accacccggt aagccgattt gacctaacat
     2101 tgcagctagg gtgactaaca tccaatgccc ttgttcaccg tgtcgttggc gttgaatccc
     2161 ccagcctgcc attaacattg tgcgtttaga ttggaaatca gtcgctaatt ttttaatggt
     2221 ttcttccggc acaccacaaa tttgacttgc ccattttgca tctttcggtt gattgtctgt
     2281 tttaccgagc agatattctt caaacttctc aaaaccggtg gtgtattttt taaggaatgc
     2341 ttgatcgtgt ttgccttcag cgactaaggt atgagccata ccgagcatta acgcaacatc
     2401 agtgccggtg tttaccggta cccattctgc acctaaaaat tcgcaagatt cactttttac
     2461 cgggtcaata cagataatgc gtttcccgct ctctttgagt tttttgaagt attcaatacc
     2521 ttgttgatcg gtagaagtcc acgcaatgcg tagcgtattt aacgggttag ctgaccaaag
     2581 caccacaata tctgaacttt ctaaaatcac ttcccaacta gtttgctgtt cgtacacctc
     2641 gattgtaccc aagacgtgtg gcataatcac ttgagcagca ccggtagaat agtcgccttt
     2701 tacaccaaca aatccgccgg taacatttaa atagcgttgt aacagtgtac gggaagcgtg
     2761 taatgcccca gaactatacc aaccgtaaga accaccaaaa atagcattcg ccccgtgagc
     2821 ctcacgaaca cgtttcattt cattcgcaac taattcaaat gccttatccc acgaaactcg
     2881 cacccattca tcacgcccac gtagagtagt atctttatta ccttctaagt agcctttacg
     2941 caccattggg tattttactc gtgtttcacc gtgtacttgg tctgctacca cgtgttgcaa
     3001 ttcatttggc acagaaatct caaaaacaga atcagatttc accactttgc cattttctac
     3061 cactacacct aaaggccccc agtgggcggc agttactact gttttcatct cggctgcttt
     3121 ggcactggtg gaagctaatg cccccactac accggtagta ccactggcta aacctgcacc
     3181 ggcagctaag cttaatgatg aattttttag aaaaccgcga cgacttacgt caatattgtc
     3241 ttgttttttc ataataaacc tcgtttgaag ttaagttaaa aatagacacc tttctaaagt
     3301 cgagtggata aaccatttct cgtgtatcca ttggtaaaag caccgacggt aatttttgca
     3361 aaatgttagg aaaaaatgac cgcttgtagt aggtgcttta cggcttgcta ccatatctct
     3421 gctctcagca gacacttagg ctcacaagct cggtatcctc taagttgcaa ttaatgggaa
     3481 ctgcctgcta catcttttgc attacgttgg agatagatag tcaccgcacg cacatcatca
     3541 gccgacatag atgttctgtc tttcattgaa tttaccaccc caatccattg atttgcggtg
     3601 taatgttctg ccccaatcgg tgcatggcaa ccgctacagt gtgtttggtt taagttatcg
     3661 ccaaaatgat ttagtgcagt aatattggca gtgactgcat ttttaggagc agtaatttca
     3721 aaattgattt ctttccaatt tgagttggtg acattatcaa acactgtttt tgtgacattc
     3781 actttgcttt gagcgtcatc aggtagaaga gcaaccataa tacgcttgcc taaatccata
     3841 tacaccacag attccgcacc ttcttgttgc cagccattaa ccgttgcttt aacctgctcg
     3901 ccttctgctg tccattgatg taattctgca taaggtaaca agcgaatttc tccaccttga
     3961 gcgagctgca ctggagtcat tgcgagcgta taaagggttt tatcatttgt gctaaattcc
     4021 gcaccgtgtt tagctaattc gcccattgct gcggcattat ctaccggcac atcaggcata
     4081 aaatgggcaa ttcctttatg gcaatcaata caggtttgat tagtttcttg ggctagtttg
     4141 tgcattttct gtgccgcttc tgtttgttca gaaagtacca tcgcatcaaa actatgacag
     4201 cttttacagg tcatagaatc gttttctttc atttctgccc aaacacgttg tgccatttct
     4261 agacgatggg cttcataggc ttcttgatca ggtaatttat tggttacaac ggtgtgccaa
     4321 acatctttta atgcgatgat tttagctttg acataatgca agccgtcttg tggtagatgg
     4381 caatccgaac actcggctcg aattccctta cggttggaaa aatggacact gccttcccac
     4441 tctgcctgtg ggtgagtcat agaatggcag ctcacacaaa attctgttga gctggtggct
     4501 ttcattgcat attgtgtgcc acctaatacc gcagcaccta atgccataat accgacaaat
     4561 acagcaattt ttttcatctt agccataaat taccccactt aatacactaa aaattaaaga
     4621 cagaggcatt gtattaactc ccaagaggta taaaaataag taaaaatacc tattacctcg
     4681 aaagagtgag aataatttga tctaaggcaa aaatatgtag tgaaatttta ggctttttca
     4741 gagaaccacg gcggtaagat aaattgtgca ttggcaaaca actccggtgc aatcacacgg
     4801 ctctgccctt gttggatttg gtaaaccaac tgtggcaagc tgatagataa gtcgtgggtt
     4861 tctgcgtaag ttaatccaat ttggctatcg gtattaaaat agtaagtgcc gtttacccca
     4921 taatgtggct gtaagcggat cgcattggag gcggctttaa aatgccgtgg tgaaacactc
     4981 tgtttccacg catttgccag aatattcgct tggtcataag caatactggc ttgggaataa
     5041 gtgggcgatt tgccgaaaaa ccgttcgtaa tgttggctaa actgtctgcc taaataattt
     5101 tcgcttaacc cactattggt actccaaaga acgccttcgc aaagtttctc gtgtggcaaa
     5161 aacgccgaag gtgcataaat actgtaaata atggcatttg ttgggttgcg aatgaacgtt
     5221 tgataaaact gtacaatatc ttctgcaaaa taagaggcaa acacaataat ggaaggtgcg
     5281 agattatgca cctgtgccat taatgtggca taattatctt gctcttgggt gagttccaga
     5341 taatccacat tccagccttg ctcccgcaaa cttagggtta aactttcaat accgatatca
     5401 actttttgcc aactcaccgt taccaccatc accgttttat tctgaagcca aggggcataa
     5461 tggtaacggt aatattgcag aaagtgagat atacctaagc cataggttac atcactggcg
     5521 caaacctgaa acatattttc aattcgttga ttcggtaaat tttgggcaag tttatttgag
     5581 ccactatggg ttgcaatatg caaataagga atgcctttag ttgcaattaa ttcgtgtact
     5641 tccggcaagt agcaggcata agcggtggaa atcgcatcaa cttcttgatc aaataaatga
     5701 tggtatgcct gaagaatact tgctttatct ttaacataaa acccagccgt tgcaacctgt
     5761 acttctctgc cgtgaatacc accttgttga ttgatggtct caaccgcaag ttgagtgccg
     5821 ttaagtagct ctcgggtatc gatttgccca ataccctgtt caacataagg cacgccaata
     5881 ataatcggac gactatgatg gtggtttatc gtaaaaatcg gtggttcgtg tcgagttgaa
     5941 gccgttacaa gcggttgttt tttcgcgata atttgcaatg ccagttgttc aatgttaaag
     6001 ctggcaagaa tggattgggt tagttttccc ggtgtgggta aacagattaa atgttcggta
     6061 atggcaaaaa cagctaataa ggttcggctt ttggtatgtg ttttcgcaaa aatattttcg
     6121 atatgtttat caacagtccg tttgctgata caaagctcat tggcgatatc ctcattgctc
     6181 aaaccactgc taattagagt gagaagctca atctcccttt tactcagtcc ataaggcaaa
     6241 tcggtatgct ctgcttgagt caatacaaac gattggtggc gttctaggct gatcttaagc
     6301 cattgatggt ttgaactata gtaaaactgc aagctatttt tatctgaata gagcaatgcc
     6361 tgtgcatagt ggcaaatttg cggtaattca gcaaaatagt ctgcccctgc tttgtcttcc
     6421 cattctgata tttcgccttg ttcatttact aaacaagacc atcggctacg tttatccatt
     6481 ctcacctcga actaaaaacg ttagctaaaa tagcaaaatt atctcggcat taatatgata
     6541 taaatcaaac taagcaacgg ttgccagatt tttataggta gagcatacaa tttaaattat
     6601 acctcttgcc taattaggtt tctcaccata aaatgaaaac caatttattg aagactccga
     6661 gcattatttc ctaactctaa aaattaaaga caaatttttg atatggaatt tatgccagta
     6721 acagcataaa gctgttcaaa ggataactta gaaatgggtt gtcctctcgt gtttagaaag
     6781 gtgtcaaatg cagtctatta tcccaattaa aaacgtgggt gatgatgtcg tttcaggttt
     6841 agttgatcgc tttcagcgtc aatatactta tttgcgacta tccattactg atgtttgtaa
     6901 cttccgttgt aattattgct tgcctgatgg ctataagccg ccttcacata agcaaacttt
     6961 tcttagcgtt gatgaaatcc agcgggtggc taaagccttt gccaatttag ggacggaaaa
     7021 aatccgtatc accggtggtg agccaacttt acgcaaagat ttcctcgaaa tcgctcacac
     7081 tatttcacaa acacccggta tccgtaaagt tgccctaacc accaacggct atcgcctaga
     7141 gcgtgatgtt gaactgtggc aacaagccgg tattaccgat attaatgtga gtgttgatag
     7201 ccttgaccca cgccaatttc aactgattac cggcgagaac aagttgcaat ctattttaaa
     7261 gggtattgat aaagcctttg aaattggcta cccaaaaatt aaagtcaatg cggtgctaat
     7321 gaagcaatat accgcccctg aattggataa attcctcact tggatcaaag acaagccgat
     7381 tcaaatgcgg tttattgagc tgatggaaac cggtgaaatg gatagtttct tccaagctca
     7441 acatctttct ggtcaatcgg tgatgacccg cttattacaa gaaggctggc aattacaacc
     7501 caaagcgatg tctgacggcc cggcaaaagt gctttctcac cctgatttta aaggcgaaat
     7561 cggcttaatt atgccctacg agaaaaattt ctgtgcctct tgcaaccgct tgcgtgtttc
     7621 cgcacttggt aaactgcatc tttgcttatt tggtgaagaa gggattgata ttcgtgattt
     7681 actggcaagc gatgagcaac agttacagct tgaaacccgc ctaaaatctt ccttacaagg
     7741 caagcgagaa caccactatt tgcatattgg ggatagtggg gttaggaata atttggcgag
     7801 tatcggtggt taaacaacca ccgagcgtgt tttttctgtt acttttcttt gcttcgccaa
     7861 agaaaagtaa ccaaaagaaa ggcgacccca gattttcctt aatccttcgc ttgttgctaa
     7921 cttttgggac gaacttttca aactcgccaa ctgcgttggc tcaaacacga aaagttgtcc
     7981 tcaaaagttt acgcaatcgc tacggcggaa aataggggca agcggtacat tatttaagat
     8041 ttttgcaagt ttttatttaa aaataaccgc ttgtaatgtt ccagcttctc ccttctgaac
     8101 cgcctgagcg actttgaatt tataggaaaa atttagtgtt tgagccaacg aagttggcga
     8161 gttttaaatt tttccgttaa acaaattcaa ataaaatgaa gatagcggtg aagtagggtg
     8221 tgctttcttt ggttactttc tttgcacaag caaagaaagt aacaagaaga cgctcaatat
     8281 taattttaca atcaaattta aagagacaaa caaatgacaa ccttcaccca catcaaccaa
     8341 aacggcgaag ccaatatggt tgatgtttca atgaaacaag aaaccgtacg cattgcccga
     8401 gcggaggcat ttgtgcgaat gaatgccgaa acattacaaa tgatcgtcag tggtaatcac
     8461 cacaaaggcg atgtattcgc aaccgctcga attgccggta ttcaggcggc gaagagaact
     8521 tgggagctga ttccactctg ccacccgtta ttgctttcta aagtggaagt tcaactggag
     8581 gcgttgccgg aaagcaacca agtgcgaatt gaatcactct gtaaactaac cggcaaaacc
     8641 ggcgtggaaa tggaggcatt aaccgcagct agtgttgctg ctctgactat ttacgatatg
     8701 tgtaaagccg tgcaaaaaga tatgattatt gaaaatgtaa gattacttag taaaagtggc
     8761 ggaaaatcag gagatttcaa ggttgattaa gatacttttt ttcgcccaag tgcgtgaact
     8821 tgtggcaacg gatagactag atgttgagcc tacttttacc actgccgaag cgttgcgtga
     8881 acacttaagc caacagggta aaaaatggca acttgccctt gaaaaaggca aactgctggt
     8941 tgcaattaat caaacgatct gcccgcttga aacggagatc aaagacggcg atgaagtggc
     9001 attcttccca ccggtaaccg ggggctaaaa tgtttgaaac cctgattgaa gtacaagaag
     9061 ctgaatttga ccaaaaccgc atttaccaat ggctcagtga acaacatagc gtgggggcag
     9121 ccactctatt catcggtaaa gtacgtgaaa tgaatttggg cgataatgtt tccggtttgt
     9181 ttcttgaaca ctatccggca atgactaaaa aagcattgca agaaattgtg gctcaagctc
     9241 gcactcgttg ggatttacaa cgcgttgcgg tgattcaccg cattgggcaa ctgcataccg
     9301 gtgatgaaat cgtattagtc ggtgtgagtt ctgctcatcg aggtgatgcc taccacgcca
     9361 acgagtttat tatggattat ctcaaaacca aagccccatt ttggaaacga gaacaaaccc
     9421 aagccggtga acgttggatt gaagggcgtg agagtgacca acaagccgcc aaaaaatggc
     9481 aatagaaaaa atggcaataa tttgcaaaag gtaacgaatg gaaaatttat atattgattt
     9541 gcagatcgcc tgcgaaaata ccgaaaactt accaagtgaa caacaatttt atacttgggt
     9601 acaaaaagcc ctcgcagttg aggcaaaaac cgatgatttc cctgagagtg agatcaccat
     9661 tcggattgtc gatgaagcag aaagccacga gctaaatctg acttatcgtg gtaaagacaa
     9721 gccaaccaat gtgctatctt tcccgtttga ggcccccgaa ggaatcgaaa tgccactatt
     9781 gggtgattta gtcatctgtc gtcaagtaat ggaaaaagag gcggaagaac aaggtatttc
     9841 agccgaatcc cattgggctc atttggcgat tcacggcaca cttcatctgc tcggctacga
     9901 ccatattgag gaagatgaag cagtggaaat ggaaagcctc gaaaccgaaa ttatgcaaag
     9961 tttagggtat gaagatccgt atctttcaga aaaaatatag tcttgtaggg gagggtcctg
    10021 tgccctccag tactccatta taatcggaca ggcacaggag ctgttcctac aagttatatg
    10081 agaacgatcg caatcataac atctcctaac aatgccgact ttattcaaca aggctatttt
    10141 tacattcccg aaaatcatca gcttaaagct atcccctttt ccaatgccaa aacgatttta
    10201 ggacaagaat acccttttgc catttatact atgcaagcag aaaatggggt gaattttaac
    10261 ttagatgctt ttgcaatact tgccggcact attcaagaaa acggtacttt atttttgctt
    10321 tgtcctcagt gggataattt ggaaaatgaa ttagatttcg atgcgttacg ttggaacgaa
    10381 aatcaggcta ttgcctgccc gaatttctat caacatttta aacaattagt cgctaaattt
    10441 ggttttgaag tcagaactaa tttgccaaaa ttaccggcta caagcggtca aattccttca
    10501 aaaatttacc aattaacgca agaacagcaa aatattttgc aaaatctacc gcttgatcct
    10561 gccgatattc atttgattac cgcccctcgt gggcggggca aatctaccct tgcagggcga
    10621 cttgcaaaag cgttacccgc acaaaataag gtattaatta ccgcacggtc ttattctgca
    10681 ttgcccagtt tttggcgttc ggcagagcaa aaaatccctt ttttcgcacc tgacaattta
    10741 atcgagcaag ttaaaagtaa aaacatttca cctaaccaat ggctctttat tgatgaggca
    10801 gctagcctac ccttgccgat actccacaca ctttgtgcct attttgaaaa agtggtttta
    10861 accacaacaa cccaaaatta cgaaggcacg gggcgaggat ttgagctgaa attcccggta
    10921 atgttggata aaccgtttaa acattggacg ttatctcaac cattgcggtg gagcgaaaat
    10981 gatcctctcg aagcatttgt gactgagttg ctattacttt ccgaagaaaa atctcccctc
    11041 tttagcaaag aggggaaggg ggagatttat tcgcttcgct cacactatgt gccgttgcta
    11101 aagcaacgtt caaacgcaaa gcgtttgtgg cagaagcaat tatcgaagaa tataagttat
    11161 tcgaataagc atttacagga aaaaatcgct ttctaccact tactcgccaa cgcccattac
    11221 aaaaccactc caactgactt acgccgttta tttgatggcg aaaatcagct tttgttttca
    11281 caacaggaaa acgagcaact tatcggcgga atttgggcaa tgcaagaggg tggtttagat
    11341 gaaaaattaa cccaagccat ttggctaggg gaacgccgtc cgcaaggcag tctagtggct
    11401 caatatctct gttttcaagg gaatttaccg caggcttgca aattgcattc cattcgcatt
    11461 tctcggattg cggtattgcc tgaaaagcaa aatcaaggcg ttggcaagcg gttgatttct
    11521 cagtttattt tgcaaaattc ggtagaaaaa cgaccgcttg attttatctc tgtgagcttt
    11581 gggctaacta ctccactcca cgcattttgg caatcttgtg gtttccaact tgtgcaaatc
    11641 acaccgaata aagaggcaag cagtggttat caaagtgcaa tgatgattta cccgatttcg
    11701 gagcaaggaa agttatttgc acagcaggca acccaagcct ttgccagaga tttcgccctt
    11761 cagccatttt tcacggaaat ttgtgaggaa ttgcaaaatt tcgaacaatt tcgaccgctt
    11821 gccacacaca atttagatga acgtgattgg aaaaatttaa ccgcgtttgc tcatggcaag
    11881 cggacgtttt cagcggcgta tgtgagttta aaacggcttt atttatcgga taaagcacgc
    11941 ttttcagagc tggatatttt tgaacgaaaa gggcaaaaaa tctccaaaat ggaggtggaa
    12001 aattatcgaa aactcgtttt gcaaaactta aacaataaat catttaaaat aatttatagg
    12061 ttttattaca tttttttgtg tttttgctct tgctaaatct gcaatttaat cgtataaatt
    12121 cactgtttat ttttcatttt gatgaggaac tccaatgtca atgtttagtc acattcaagc
    12181 tgctccggca gactcaattt taggcttagg tgaagcgttt aaagcagata gccgtgaagg
    12241 taaaatcaac cttggtatcg gtgtttatat gaccgatgaa ggcaaaaccc cgattgtcaa
    12301 agcggtaaaa gaagcagaaa aacgtttatt agagacggaa aacagtaaaa attatttaac
    12361 cattgacggt gttcaagcat tcaatgcggc aacgcaagcg ttattatttg gcgaaggggc
    12421 tgaagtgatt acctcaggtc gtgctaaaac tgcacagagt ttaggtggga caggggcatt
    12481 acgcattgcg gcggaattta tcaaacgtca tacgaatgcg aaaaatgtat ggatttcaac
    12541 cccgacttgg ccaaaccata acggcatttt tgatgcagta ggcgtgaatg taaaaggcta
    12601 tcgctactac aataaagaga ctaacggttt agattgggaa aatttaattg ccgatttaag
    12661 ccaagcggaa gccggagatg tggtgttatt gcacggttgc tgccacaacc cgaccggaat
    12721 tgacccaaca cctgcacaat gggaacagtt agcagcatta tctgccgaaa aaggctggtt
    12781 gccgttattt gactttgctt atcaaggctt tgcaaacggt ttagaggaag atgcttacgg
    12841 tttacgagct tttgtgaaaa ataaccgtga attgttggtc gcaagctcat tctcgaaaaa
    12901 ctttgggtta tataacgagc gtgtcggtgc atttacccta attgcagata atgctgatga
    12961 tgccaaccgt gctttcactc aaatcaaatc gattattcgt gtactttact ctaacccatc
    13021 tgctcacggt gcaagtgcag tagcagttgc attggctgat cctgacctta aagcacaatg
    13081 gattgcggaa ttagatgaaa tgcgtaaccg tattaaagaa atgcgtagcc aattagtgca
    13141 gaaattaaaa gaaaaagggg caaacaaaga cttctctttc attactgagc aaaatggtat
    13201 gttctcattc agtggcttaa caccggagca agtggataag ttaaaagatg agtttgcaat
    13261 ttatgctgtg cgttcaggca gaatcaatgt agcaggcatt accagcaaaa acattgatgc
    13321 tcttgccgat gcgattgtga aagtgcttta attaaacctt aaagatatat agaggcataa
    13381 gcctgagtgc ttgtgcctct tttttatctt ttaggtacaa gtgtggacac ttgcaccaac
    13441 gttgattatt cgtagaacta ggtttccaac caaagcataa aatctgctac cgtatgaaat
    13501 gagccgaaca ccagcacaat atcattttcg ttggctgttt caaacaatac tcggcttgcc
    13561 tgtgccacat tttcatagct acaagcggtt gaatttggca aaacgtttac caatttcgct
    13621 aacaccgcct cgcctgtttg tccacgccaa caatctagcc ctgcacaatg ccattcatca
    13681 ataatattag ctaatggctc gacaataccg gataaatcct tatcttctaa cgcactaaac
    13741 accgcataga ttttttgatt tggttgcttt aactcccgca gtttgtcagc taaataacgt
    13801 gctgcgtgtg ggttatgccc tacatcaata attacttttg cagccggttt aggttgagaa
    13861 aaatgagtaa aatcggtatc cgtcagggtt tgaaaacgcc ctgtcatttg agcttcattt
    13921 aaggcttgtc gaataatctt ttcatcaatt tcaaatggca gttgcattaa tactgctaat
    13981 gcggtgccgg cgtttggaat cggaataagc gggattggta aatcggtaaa ggatttttct
    14041 tcagaatacc aatgaaacag attattctta atttcaaact gccagtcttt accccggtaa
    14101 aaagcgtgac aatttaacga atcagcaatc tgtttaatcg aatgagggca atcaggctcg
    14161 ccgatgacaa ccggaatatt ttcacggaaa atgccggctt tctcacgccc gatgtcttca
    14221 cggttatcgc ctaaaaatgc aacgtggtcg atatcaatcg agataatcac ggcaaggctt
    14281 ggatcaataa tattagtggc atctaatcgc ccacctaatc cgacttctaa aatcacaata
    14341 tcgagctgat tttgtttgaa tagctcaaat gcagacagcg tactaaactc aaaataagtg
    14401 agtgaagcgg tcttttttgc attaattttt gcaaatgctt ggacgtggtc ggcatcaggt
    14461 aatacttcgc cgttaattcg cacccgttca ttatagtgaa ttaagtgcgg tgaagagtaa
    14521 acaccaaccc gaaatccggc ttttaataag gtaacttcta acaaacggca ggtcgaaccc
    14581 ttgccgttag taccggcaac ggtaatcaca taaggggcag gcttgagtaa atccagctct
    14641 gttgtgacct tacgaatacg atctaagccc atatcaatcg gtttagaatg ggcttgttct
    14701 aaataggaaa gccacgtttc caaagaatcc gtggctttag gtatgcttaa tgtattcatt
    14761 cagatatttt attctgcgtt ttctacaacc aattccgcca ctttaaacgg cgttggttga
    14821 ttggttaatt ttgcacaaag acgggaaagg gtatcacgca tctctttacg atgcacgatc
    14881 atatcaactg ccccgtgttc taataagaac tcggcacgtt ggaaaccttc cggcagtttt
    14941 tcacgtacgg tttgctcaat tacacgagga ccagcaaagc cgattagggc ttttggctca
    15001 gcaatattca catcgcccag cattgccaaa cttgcggaaa ccccacctaa agttgggtcg
    15061 gttaatacag aaataaacgg tacgccctgc tctttcattt tggctaaaat cgcactggtt
    15121 tttgccattt gcattaaaga gaataacgcc tcttgcatac gagccccgcc cgatgccgaa
    15181 aaacagataa acggaatttt ttcagctaaa gctttttccg cagctttcac aaattttgca
    15241 cctactacag accccattga accgcccata aactcaaagt taaaggaggc cgtaaccacc
    15301 ggcatattat gcaatgtacc ggcaagcacg ataaaagaat ctttctcacc ggtttgtttt
    15361 tgcgcggcag ttaaacgatc tttatatttc tttaaatctt taaatttcaa cacatcttcc
    15421 ggctctaagt cggcagcgat ttcgaccgca gaatctttat ctaacaaatt caataaacgg
    15481 gtgcgggcat caatacgcat atgatgatcg cattttggac aaacctgttg gttgcgttgt
    15541 aattcttcgc tatagagaac ttgttcacaa cttgtgcatt ttgtccatac accttccggc
    15601 acttttgatt ttgcattgct tgatgatgaa gagtctctac ctaaaattct gtctaaccag
    15661 ctcattatta ttccttttgg attgagttgt tgggttcact aaatggttgc tattaaagca
    15721 taatttatcg gtttttgata tgttttcttg acatatcaga tcactaacta tgtgtataag
    15781 ttcgataaaa aaacgcaatc agaagaatga ttaacatcac cacaatttga attaagcgtt
    15841 ttttggttaa tttttccgct gccattgctc tgctcatact taataaattt ccgcattata
    15901 agcgaaaaag cacgggctat gtagaatttg caaaattttg atcaaaaata accgcttgta
    15961 aaaaggaaat actatggcaa ccattgaaca atttcttgag gttgtaaacc aacttcgtca
    16021 ccctgaacac ggctgcccgt gggatctcaa acagaatttt gacacgatgt tccccactta
    16081 ttggaagaaa cctacgaagt agcggaagcg attcacaccc aagatcgcac tgccttgcgt
    16141 gaagagttag gcgatttatt actgcaagtg gtatttttaa gccaattagc gaaagaggaa
    16201 ggcagtttca cttttgatga tgtagtaacg gatattcacg ataaactgat ttaccgccac
    16261 cctcacgttt ttggtgatgt gaaagctact aacagcgaag aggccttaca aagttgggaa
    16321 gcacaaaaag cgaatgatgc aaaacaccgt agccaagatt ctatttttag atgatttacc
    16381 ttttgctttg cctgctctta cccgtgcaaa taagctacaa aaacggtgtg caaaagttgg
    16441 attcgattgg gataaccctc acgatgtgct ggcaaaagtc gaagaagaac tcgatgaagt
    16501 caaagcagaa atcgcacaat atccggctcg ctccatcgag cttgctgaag aattaggcga
    16561 tctcttgttt gcgattgtaa atttatgccg ccattatcat accgatgccg aggaaatttt
    16621 acgcaatgca aatctaaaat ttgaacatcg ctttcgaaaa gttgaaaaat ctgtgaaaaa
    16681 atcgggaaga actgtaaaag aatgtaaatt agaagaacta gatgtaattt ggcacaaaat
    16741 aaaagtggta aaata
//
LOCUS       DANLXS010000035        16524 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.035, whole genome shotgun sequence.
ACCESSION   DANLXS010000035 DANLXS010000000
VERSION     DANLXS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16524
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.035"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(107..622)
                     /locus_tag="PXD71_000865"
     CDS             complement(107..622)
                     /locus_tag="PXD71_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL6291011.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GARREVTEWHRIVFYRRQAEVCGQYLRKGSQVYVEGRLKTRKWQDQNGQDRYTTEIQG
                     DVLQMLGSRQGNDMGGQQGGWGNSAPAGNSYNQGNSNYGYNQTQSAAPQSKPAPQPEP
                     AMNNFDDDIPF"
     gene            777..3605
                     /gene="uvrA"
                     /locus_tag="PXD71_000866"
     CDS             777..3605
                     /gene="uvrA"
                     /locus_tag="PXD71_000866"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL6291012.1"
                     /translation="MQHIDIRGARTHNLKNINLTLPRDKFIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTISQMVDRVLEEPEGKRLMLLAPVVKERKG
                     EHVKLLENLTASGYLRARIDGEICDLSDPPALELQKKHTIEVVVDRFKVRADLATRLA
                     ESFETALDLSGSTAIIADMDDPNAEEIVFSSSFACPHCGYSLHELEPRLFSFNNPAGA
                     CPTCDGLGVEQFFDAKKVVQNEEVSLAAGAIKGWDRRSFYYFGLLKAVAEHYGFDLET
                     PFGQLPKKYQNIILNGTKEEIEFVYVNDRGDKVKRKHAFEGVLNNMARRYKETESNSV
                     REELAKYINNRPCMDCEGSRLRREARYVFLDKTNLPMVSEKSIGEALTFFEELKLAGQ
                     KEKIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVM
                     YVLDEPSIGLHQRDNERLLNTLVHLRNLGNTVIVVEHDEDAIRAADHIVDIGPGAGVH
                     GGQVIAQGTAAEIMQIEESITGQFLSGKQKIEIPAQRVPYEPTKLLTLSGASGNNLKK
                     VKLEIPVGLFTCITGVSGSGKSTLVNDTLFPLAQNALNRAENSDVAPYESIDGLSHFD
                     KVIDIDQSPIGRTPRSNPATYTGLFTPIREIFAGTQEARARGYNVGRFSFNVRGGRCE
                     ACQGDGVIKVEMHFLPDVYVPCDHCKGKRYNRETLEIRYKGKTIHQVLEMTVEEAREF
                     FAPMPMIARKLQTLMDVGLSYIRLGQSSTTLSGGEAQRVKLATELSKRDTGKTLYILD
                     EPTTGLHFADIKQLLGVLHRLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEI
                     IATGTPEEVAKDKRSHTARFLKEILAKG"
     gene            complement(3697..5430)
                     /gene="dauA"
                     /locus_tag="PXD71_000867"
     CDS             complement(3697..5430)
                     /gene="dauA"
                     /locus_tag="PXD71_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDL6291013.1"
                     /translation="MLNKWFLTKNAFLAVKPFSALKDALNAGYGKQHLIRDIISGLTV
                     GVIAIPLSMALAIASGVPPQHGLYTAIVAGIVIALTGGSRFNISGPTAAFVVILYPVT
                     QQFGLSGLLMATMLSGGILVLMALLRLGRLIEYIPLPVTLGFTCGIGIVIATLQIKDF
                     LGLSIEKMPEHYLGKLEAIFSALPTINWADTLVGVVTLLVLTRWHKLRLPIPGHLPAV
                     FIGTLLSLLLIHFGQNVATIGSAFHYTLSDGTTGYGIPSVLPEFVLPWNLPNAEGNRI
                     DWNLTTIQALLPAAFSMAMLGAIESLLCAVVCDNMTDTKHHSNNELLAQGLGNIASPF
                     LGGITATAAIARSAANVRSGAVSPISSVVHALLVLFALLFLAKALSYLPLSSMAALLL
                     VVAWNMADIPQIIQLTRRSGKNEIAVLLTCITLTVLFDMVIAITVGVLLASLLFIRTI
                     AEMTKSFEMAHPTDLNDVLVYRISGPLFFAAADNLFADLHNKTVHTDHQIKHIVLQCD
                     AVTVLDAGGIHALTRFVQHMLPHQQLHLCNVQFQPMRMLVRSQKTVNEIPQIQFSSDL
                     ESAFDKIRQAN"
     gene            5672..6604
                     /locus_tag="PXD71_000868"
     CDS             5672..6604
                     /locus_tag="PXD71_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6291014.1"
                     /translation="MDSLIHFLSAFLTNITWIGALELGLIYSLVALGVLISYKILDFP
                     DLTADGSFPLGGAVCVISILNQVDPWLATLYGTLAGAVAGMVTASLHIGFKIEKLLAS
                     ILMMIALYSINLRIMGMPNIAILGETTIYDVMPIESDLQLALVRLVMALLVVIIVKGL
                     FDLFFATEIGLAVRATGTNSRMAKAQGIAINKMTILGMAISNALIALGGALYVQSNGG
                     VDISIGVGTIVIGLAAVIIGEAIFSVKRIIWLTFAVIIGSILYRFFIALALNNETLNG
                     IGFGPQDLNLITALLVVIVLAVPRIKHKLLGKRG"
     gene            6606..7403
                     /locus_tag="PXD71_000869"
     CDS             6606..7403
                     /locus_tag="PXD71_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6291015.1"
                     /translation="MIELNNLFITFNKGTAIENPVLRGLSLKVEQGEFVSVIGSNGAG
                     KSTLLNAISGDCAVDSGEILIQGNKVNQSTTWQRANQVARVFQDPMAGTCESLTIEEN
                     MALAYQRGRKRGLGFAIKRQMRELFKDKLALLGLGLENRLTDQMGRLSGGQRQAVSLL
                     MASLQPSNILLLDEHTAALDPKTTDFVLELTNKIVREQKLTTLMVTHSMRQALDYGDR
                     TVMLHQGQVAFDVSGEQRQKMDVPDLLQLFQQSRHEQLSDDGLLLGN"
     gene            7490..7711
                     /locus_tag="PXD71_000870"
     CDS             7490..7711
                     /locus_tag="PXD71_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291016.1"
                     /translation="MLSIFKTPVEKETLDDWAKISVDVAKVAILAIPVILFGKEPTLI
                     KLTNLALLVLSIYVFLTLGRKLRQLKEVL"
     gene            7894..9819
                     /locus_tag="PXD71_000871"
     CDS             7894..9819
                     /locus_tag="PXD71_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL6291017.1"
                     /translation="MTKYDDKIQHAFSENGLLSQNISGFRPRQAQLEMAQVVAKAVKF
                     ATPVVVEAGTGTGKTFAYLVPALLSGKKTILSTGSKNLQDQLFNRDLPTIQKALKYKG
                     KIALLKGRANYLCLERLDQVTAMGVLGDKSVLTDLKKVSKWQNATKTGDLSECITIAE
                     DSPILPQLVSTTESCLGSDCPHFKDCYVVQARRKAMEADVVVVNHHLFCADMAVKKTG
                     FGELIPEAELVIFDEAHQLPDIASQYFGQSLSSRQLFDICKDTNIVYRTELKDLSQLG
                     KAADHLTKVVQDFRLLMGGEGQIRGNLRELFRDKKVVDGLVKVAENIDFLSEVAKKSL
                     GRSETLDKIFERLAEVKVQLKKLMDTSVTGYCYWYEANGRSFGLHITPLMVADKFGEQ
                     LKNQQIGWVFTSATLEVGGKFDHFCQRLGIENAEQLVLQSPFDYQHQSLLCVPRYLPD
                     TNKSNTLTALGQLLKPVIEANKGRCFLLCTSYYMMRGLADFLREHSSLNVLLQGETSK
                     SRLLEKFVKEDHSVLVATQSFWEGIDVRGDDLSLVIIDKLPFTSPDEPLLKARMEDCK
                     IQGGDPFNDIQIPEAVITLKQGVGRLIRDVTDRGVVIICDSRLVMRNYGQTFLKSLPP
                     SRRTRDLGNVIQFLKQK"
     gene            9893..10258
                     /locus_tag="PXD71_000872"
     CDS             9893..10258
                     /locus_tag="PXD71_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL6291018.1"
                     /translation="MLSTLLRMVASLALLFVMLYLGKLIVYLFPIGIPDSILGMLILF
                     GCLVIGIVKVEWVIPSAKPLTRYITLFFLPICVELIEHLELLRQNLNTFVLATALSTL
                     VSVVAIGVFAQWIFHYKRG"
     gene            10260..10946
                     /locus_tag="PXD71_000873"
     CDS             10260..10946
                     /locus_tag="PXD71_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL6291019.1"
                     /translation="MSYLYTVLTVIMFIAGLKISQKLKSSFLNPFLIALALLIGVLLI
                     FNIPYQDYYQGNSPLNNLLGVSVVALAYPFYEQLPHIRKKWRQIVVILLFSTIITMIT
                     GLGFSLLFGASQEILAAILPKSVSMPMAIIITNEINGNVAIAAVGVMIAGLVGSILGF
                     TLLRLAKVRNVMVIGLSMGAASHALGTARCLEHSMKAGSYSSIALVACGVLSSIFAPL
                     IFNVIVTLCY"
     gene            11030..12793
                     /gene="msbA"
                     /locus_tag="PXD71_000874"
     CDS             11030..12793
                     /gene="msbA"
                     /locus_tag="PXD71_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDL6291020.1"
                     /translation="MQEQITQEKDLSTLKTFKRLWPTISPFKAGLITGAVALVLNALV
                     DSSLIYLLKPLLDEGFGQANNDFLKLMAVLVVVFILVRGITNYIASYCLAWVSGKVVM
                     TLRRHIFQHLMYMPVSYFDNNPTGRLLSRVTYDTEMVASSASHSLVTIVREGAYLLSL
                     FVVMIYTSWQLSIVLFVMAPIIGVLISFVSKYFRQLSRNIQSSMGELTVTTEQMLKGH
                     KVVLSFGGQEIEKARFDRVSNDMRRKGMKVVSADGISDGVVQLIASFALSAVLYVATF
                     PEVMSENLTAGSFTVVFSSMMAMLRPLKSLTNVNSQFQRGMAACQTLFEFLDLKTEKD
                     HGLISVAKAEGNVEFKKVSFSYEGKEEKALDNISFTIPKGKTVALVGRSGSGKSTIAN
                     LLTRFYDVTDGQILLDGINIQDYRLKNLREQCSVVSQQVHLFNDTIANNIAYAAIDKY
                     SREEIIAAAKAAHAMEFIEKLENGLDTVIGENGASLSGGQRQRLAIARALLRNSPVLV
                     LDEATSALDTESERAIQSALEVLQKDRTVLVIAHRLSTIEKADEILVVDHGNIIERGS
                     HTELLAQGGAYKQLHSLQFGA"
     gene            12882..13514
                     /gene="lolB"
                     /locus_tag="PXD71_000875"
     CDS             12882..13514
                     /gene="lolB"
                     /locus_tag="PXD71_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDL6291021.1"
                     /translation="MKKLTKLLSLSALLVLTACNSVLNASTEVQQPTVQIPHDDPQWQ
                     QHLAKLSQIQGYTATGQFGYISPEERFSSHFNWRYTNPIQFGLELSSNLSTKSLKLER
                     NKRGLTVSDNEGNSRSDRDVDALMQEIIGVSFPIDQFAYWVKGLPEKDGNYIVNEKRQ
                     LSQFSYPLNGTLWKASYVEYHEDRVPNLPKLIVLENGTQTLKIRIEKWAY"
     gene            13502..14356
                     /gene="ispE"
                     /locus_tag="PXD71_000876"
     CDS             13502..14356
                     /gene="ispE"
                     /locus_tag="PXD71_000876"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL6291022.1"
                     /translation="MGVLMKKVTLPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGD
                     EIEIEITESSEVELINPVEGVATEENLIYRAAKLLQKTTACRQGCRIGIKKNLPMGGG
                     VGGGSSNAATVLVGLNHLWNTELSLEKLAELGLSLGADVPIFVRGMAAFAEGVGEVLT
                     PCDPQEKWYVVLKPEVSISTAAVFTDPNLPRNTPKRPLAELLSLPWENDCEKVVREKY
                     SAVEDLIQELLQYAQFRLTGTGACIFAEFETKAEAEQVFAHKPEAVFGFVAKGQNISP
                     LHQMLFQI"
     gene            14382..15332
                     /locus_tag="PXD71_000877"
     CDS             14382..15332
                     /locus_tag="PXD71_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDL6291023.1"
                     /translation="MTDIKLFAGNATPELANRIAKHLYTSLGNATVGRFSDGEIQVQI
                     NENVRGGDIFIVQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVIADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLIDDILKKS
                     DLVNPIVVSPDIGGVVRARAVAKLLNDTDMAIIDKRRPKANVSQVMHIIGDVADRDCI
                     LVDDMIDTGGTLVKAAEALKERGARRVFAYATHAVFSGTAAQNLANPALDEIVVTDTI
                     PLSNEIKALNKVRVLTLSGMLAEAIRRISNEESISAMFDA"
     gene            15410..16420
                     /gene="pyrD"
                     /locus_tag="PXD71_000878"
     CDS             15410..16420
                     /gene="pyrD"
                     /locus_tag="PXD71_000878"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDL6291024.1"
                     /translation="MYSLIRKLLFALDTEKAHQFSIQALALAGKSPFSILPTPDNPTQ
                     VMGLTFKNPIGLAAGADKNGEAIDGFGKLGFGFIEVGTITPLAQDGNPKPRQFRIVDA
                     EGIINRNGFNNKGVDVLVENVKNATYSGILGINIGKNAVTPIEHSFDDYQICLRKVYQ
                     YADYVAVNISSPNTKNLRSLQYGEALDDLLRNLKNEQAQLSQKMGKYRPLVLKIAPDL
                     TDEEIVSVADSLIRYQIDGVIAGNTTLSRESIAGLPYSEQLGGLSGKPLHSLSTRLIS
                     KLSAELGERIPIIGCGGVHSLESGQEKVNAGASLLQVYSAMIYQGPSLIQTLAKHLQL
                     NS"
BASE COUNT         4754 a         3126 c         3824 g         4820 t
ORIGIN      
        1 gttccctatt attttgagta aggtaggggc gctttatatg agtagctttt tatttcatcg
       61 aaatgcggtg tatggaaccc atgagctact taagtcaata ctttgattag aacggaatat
      121 catcatcaaa attgttcatt gccggctcag gctgtggtgc cggtttagat tgcggtgcag
      181 cactttgggt ttggttgtaa ccataattgc tgttgccttg attgtagcta ttacccgcag
      241 gagcagaatt tccccaaccg ccttgctgac cgcccatatc attaccttga cggctgccga
      301 gcatttgtag cacatcgcct tgaatttcgg tggtgtaacg atcttgcccg ttttggtctt
      361 gccatttacg ggtttttaaa cgtccttcta cgtaaacttg agagccttta cgtaaatatt
      421 gcccgcatac ttctgcttga cggcggtaga atacaatacg atgccactcg gttacttcac
      481 ggcgtgcacc ggtgttttta tccgtccagc tttctgaggt tgccacacta atatttgcaa
      541 ccgcttcgcc atttggcatt gtacgcattt cagggtcgtt acctaaatta ccgacaataa
      601 ttactttatt gataccagcc ataaaatcct ctgtttgata aattaaacta aaatttgcta
      661 agtattctac ctgaaaatcc aaaagatgaa caccaaaaaa ctgatatttt gtacagtttt
      721 attaaaatat gcgataatga tcgaaaattg agaaaatagc actggacatt aaaaatatgc
      781 aacatatcga tattcgtgga gcaagaacac acaacttaaa aaacatcaat ttaaccttgc
      841 cgagagataa attcattgta attaccgggc tttccggctc gggcaaatcg tctctggcgt
      901 ttgatacgct ctatgcggaa ggacaacgcc gttatgtaga atcactgtct gcatacgccc
      961 gccagtttct ctccttaatg gaaaagcctg atgtggatca tattgaaggg ctttcccctg
     1021 cgatttcgat tgagcaaaaa tcaacctctc acaacccacg ttcgaccgta ggaacggtaa
     1081 cggaaattca cgactattta cgtttattgt ttgctcgagt gggcgagcct cgttgccctg
     1141 accacgatgt gccattggct gctcaaacta tttcgcagat ggtggatcga gtattggaag
     1201 aaccggaagg aaaacggttg atgctacttg cccctgtggt gaaagagcgt aaaggcgaac
     1261 acgtaaaatt attggaaaat ttgaccgcta gtggctattt gcgggctaga attgacggtg
     1321 aaatttgcga tttatccgac ccaccggcat tagaattaca gaaaaaacac accattgaag
     1381 tggtggtgga tcgctttaaa gtaagggctg atctagccac ccgtttggcg gaatcttttg
     1441 aaacagcgtt agatttatcc ggctctaccg caattattgc agatatggac gacccaaatg
     1501 ccgaagaaat tgtgttttcg tccagctttg cttgtccgca ttgtggttac tctttacacg
     1561 aattagaacc acgccttttc tcgtttaaca acccggcagg tgcttgcccg acttgtgatg
     1621 gtttgggcgt tgagcagttt tttgatgcca aaaaagtagt gcaaaatgaa gaggtttcat
     1681 tagccgccgg tgcgatcaaa ggctgggatc gccgcagttt ctactatttt ggcttattaa
     1741 aagccgttgc cgagcattat ggctttgatt tagaaacccc attcgggcag ctaccgaaaa
     1801 aatatcaaaa cattatttta aacggcacta aagaagagat tgagtttgtt tatgtcaatg
     1861 accgaggcga taaggtcaaa cgtaaacacg cttttgaagg ggtgctgaat aatatggctc
     1921 gccgctataa agaaaccgaa tcaaactcgg tgcgtgaaga gttggcgaag tatattaata
     1981 atcgtccttg tatggattgc gaaggctcac ggttacgccg tgaagcccgt tatgtattct
     2041 tagataaaac caatttgccg atggtttcgg aaaaaagtat cggcgaggca ttaacctttt
     2101 ttgaagagct gaaattagcc ggtcagaaag aaaaaattgc tgagaaaatc ctcaaagaaa
     2161 ttcgtgaacg gctacagttt ttagtgaatg tcggcttgaa ttatctttct ctttcccgct
     2221 cggcagaaac cctttcaggt ggcgaggctc agcggattcg ccttgccagc caaattggtg
     2281 cgggtttagt aggggtaatg tatgtgctgg atgagccttc catcggcttg caccaacgag
     2341 acaatgaacg cctactgaat actttggtgc atttacgcaa tttaggcaat accgtgattg
     2401 tggttgaaca cgatgaagat gcgatccgag ccgcagatca tatcgtggat ataggcccgg
     2461 gtgccggtgt acacggtggg caagttatcg cacaaggtac ggcagctgaa attatgcaaa
     2521 ttgaggaatc tattaccgga caattcctct caggcaagca aaaaattgag attccggctc
     2581 agcgtgtgcc ttatgagcca actaaattgc tcaccttatc gggagcaagt ggtaacaact
     2641 tgaaaaaagt gaaattagag attccggttg gtttatttac ttgtatcacg ggtgtatcag
     2701 gttcgggcaa atcaacttta gttaatgaca cactattccc tctggcacag aatgcgttaa
     2761 accgagcgga aaacagcgat gttgctcctt atgaatcgat tgacggctta tcgcattttg
     2821 acaaggtaat tgatattgac caaagcccga ttgggagaac tcctcgctca aatccggcaa
     2881 cttacaccgg attatttacg ccaattcgtg agatttttgc cggcacgcaa gaagctcgag
     2941 ccagagggta taacgtaggg cggtttagct ttaatgtgcg gggcggacgt tgcgaagcct
     3001 gtcaaggtga cggcgtgatt aaagttgaaa tgcacttctt gcctgatgtg tatgtgcctt
     3061 gcgatcactg taaaggcaaa cgctataacc gtgaaacctt ggaaattcgc tataaaggca
     3121 aaactattca ccaagtgtta gagatgacgg tggaagaggc gagagaattt tttgccccta
     3181 tgccgatgat tgcccgcaag ttgcaaacct tgatggacgt ggggttatct tatatccgtt
     3241 tagggcaatc ttctactacg ctctcaggcg gtgaagccca acgggtgaaa ttggcgaccg
     3301 agctttccaa acgagacacc ggcaaaacgc tctatatttt agatgaaccg accaccggct
     3361 tgcattttgc cgatattaaa cagttactcg gcgtgctaca tcgcctgcgt gatcaaggca
     3421 atacgattgt ggtgatcgaa cataatttag atgtgattaa aaccgcagac tggattgtcg
     3481 atcttggccc tgaaggtggc agtggcggcg gtgaaattat tgctaccggc acaccggaag
     3541 aagtggcaaa agacaaacgt tctcacaccg cacgcttctt aaaagagatt ttggctaaag
     3601 gctaacgttc aacacaagcg gtcatatttt cggaaaatgt tgcaatttgc aaaaagttgg
     3661 aaaatatgac cgcttgtttt atatccggag ttattcctag ttagcctgtc tgattttgtc
     3721 gaaggcagat tcaagatcgc tactgaattg aatttgcggg atttcgttta ccgttttttg
     3781 tgagcggact agcattctca tcggctgaaa ttgcacatta cagagatgaa gttgttgatg
     3841 tggcaacata tgctgtacaa agcgggttag ggcgtgaatg ccaccagcat ctaatactgt
     3901 aacggcatcg cattgcagca caatatgttt aatttggtga tcggtatgta cggttttatt
     3961 gtgcagatcg gcaaagagat tatcggcggc agcaaaaaat aacggaccgc tgatccgata
     4021 aaccaacaca tcgttcaaat cggttgggtg agccatttca aaggatttag tcatttctgc
     4081 aatagtacgg ataaatagca ggcttgccag taacacccct accgtaatgg caattaccat
     4141 atcaaataat accgtgaggg taatgcaagt cagtaaaacc gcaatttcat ttttacctga
     4201 acggcgggta agttgaatga tttgtggaat atctgccata ttccaagcta ctaccaataa
     4261 tagtgcagcc attgaggaaa gaggcaaata ggaaagtgct ttagccaaaa agagtagggc
     4321 aaataaaacc aacaaggcgt gaaccacact agaaatgggg gaaactgccc cggaacgaac
     4381 attggcagcc gatctcgcaa tcgctgctgt ggcggtaatt ccccctaaaa atggcgaggc
     4441 aatattacct aagccttggg ctaatagttc gttgttagag tggtgcttgg tatctgtcat
     4501 attatcgcac actaccgcac ataataacga ttcgatagcc ccaagcattg ccattgaaaa
     4561 ggcagccggt aataaggctt gaatggtggt taagttccaa tctatccgat tcccctctgc
     4621 attcgggaga ttccaaggta acacaaattc aggtaatacg cttgggatac catacccagt
     4681 ggtgccgtca gagagcgtgt agtggaatgc tgagccgata gtggcaacgt tttgtccgaa
     4741 atgaatcagc agtaatgata acagcgtgcc gataaagacg gcgggtaaat gcccgggaat
     4801 aggcaaccgt agcttgtgcc aacgagttaa aaccaacaat gttacgacac ccactaaggt
     4861 gtcagcccaa tttatggttg gcaatgctga aaaaatagcc tctagcttgc caagatagtg
     4921 ttcaggcatt ttttcaatgc ttaagcccag aaaatctttg atttgcaagg tggcgatcac
     4981 aataccgatg ccacaggtaa aacccagtgt gacaggtaga ggaatgtatt caattaagcg
     5041 tcctaatcgg agtaacgcca tcaatactaa aattcctccg gaaagcattg ttgccatgag
     5101 taatccactt aagccaaact gctgcgtgac ggggtataga atcaccacaa aagcggctgt
     5161 tgggcctgaa atattaaatc ttgaaccgcc tgttagggca atcacaatcc cggcaacaat
     5221 cgccgtatat aaaccgtgct gtggtggcac accgctggca atcgccaacg ccatagataa
     5281 cggaatagca ataacgccaa cggttaagcc tgaaataata tctctgatta aatgttgttt
     5341 accataacct gcattgagag catcttttaa tgcactaaaa ggcttgacgg caagaaaagc
     5401 atttttagtt aaaaaccatt tatttaacat agaatgttcg agtgaagaga atgtttcgct
     5461 tattttactg caatgaataa aattaaattt gagctaacgc aagaaaaagc aggaaagacg
     5521 aaatatgttg gagacaagcg gttgttttag gcgaaaaatt tgcaaataca aataattagc
     5581 tgattttatt ttgaaaatag cgtaaaatgc cactttaatt tgtactacta tagtagtgca
     5641 tttgtgtgat ttcattaact ataataaaca aatggacagt cttattcatt ttttgagtgc
     5701 ctttttaact aatatcactt ggattggggc gttagagctg gggttaattt attcccttgt
     5761 tgcccttggg gtattaattt cttataaaat tctggatttt ccggatctga ccgcagacgg
     5821 cagtttccct ttaggagggg cggtgtgtgt gatcagcatt ttgaatcaag ttgatccttg
     5881 gctggcaact ttatacggca cgcttgccgg agcagtagcc ggtatggtta ctgcaagttt
     5941 gcatatcggc tttaaaatcg aaaaattgct tgccagtatt ttaatgatga tcgcactcta
     6001 ttcgattaat ctacgcatta tgggaatgcc gaatattgct attttaggcg aaaccacgat
     6061 ttacgatgta atgccgattg aaagcgatct acagttagcc ttagtacgat tagtgatggc
     6121 attgctggtt gttattatcg taaaaggttt gtttgacctc ttttttgcga ccgaaatcgg
     6181 tttggctgtg cgggcaaccg gtactaatag tcgaatggcg aaagcacagg gtattgcgat
     6241 taataaaatg acgattttag ggatggcgat ttctaatgcg ttaattgcct taggtggtgc
     6301 tttgtatgtg caaagcaacg gtggtgtaga tatttcgatt ggggtaggaa ctatcgtgat
     6361 tggcttggcg gcggtaatta tcggggaagc aattttttcg gtaaaacgga ttatttggct
     6421 tacctttgcg gtgattattg gctcaattct ttatcgtttc ttcatcgcac tagcattaaa
     6481 taacgaaact ttaaacggca ttggctttgg cccgcaagat ctgaatttaa ttacggcgtt
     6541 attggtggtg attgtattag cggttcctcg aattaaacac aaattgctcg gtaagcgagg
     6601 ttaatatgat tgaactcaat aacttattta ttaccttcaa caaagggaca gcaattgaga
     6661 accctgtgct gagaggctta tccctaaaag ttgagcaagg cgaatttgtg tcggtaattg
     6721 gtagcaatgg ggcgggtaaa tctaccttat taaatgcgat cagtggtgat tgtgcggtgg
     6781 actccggtga gattttaatt caaggaaata aggtaaacca atccaccact tggcaacggg
     6841 caaatcaagt cgcccgagtt ttccaagacc cgatggctgg cacttgtgaa agtttaacga
     6901 ttgaagaaaa tatggcactg gcttaccaac gtggacgtaa acgtgggttg ggctttgcga
     6961 tcaaacgcca aatgcgagag ttattcaaag acaaacttgc tttgttaggg ctaggtttgg
     7021 aaaatcggct tactgatcaa atggggcgtt tatcaggcgg gcaacgccaa gcggtgagct
     7081 tgctgatggc atcacttcag ccgtctaata tcttattgtt agatgaacac accgcggcac
     7141 tggatccgaa aaccaccgat tttgtgcttg aacttaccaa taaaattgta agagaacaga
     7201 aattaaccac gctaatggta acgcattcaa tgcggcaagc ccttgactat ggcgatagaa
     7261 ccgtgatgtt acaccaaggg caagtggcgt ttgatgtttc gggcgagcaa cggcaaaaaa
     7321 tggacgtgcc ggatctgtta caacttttcc aacaaagtcg ccacgagcag ttgagtgacg
     7381 atggattatt actcggcaat taattttgcg atctgcatcg aaaaaatgaa tttctccttt
     7441 tgtttttggc tgtcaaattt taaataataa gcccaaaagg aggtattcaa tgttaagcat
     7501 tttcaaaaca ccggtagaga aagaaacgct agacgattgg gctaaaattt cggttgatgt
     7561 agcgaaagtt gcgattttgg caattcctgt tatacttttt ggcaaagaac caacgttgat
     7621 taaattgact aacttagcat tattggtatt gagtatttat gtttttttga cgcttgggcg
     7681 aaaattacgg cagctaaagg aggtgttgtg atggaactag gctggaattt aaccataggt
     7741 ttgctaattt taaacgtctt tgcgtttgct tggctttata ttattaaccg gatttctaaa
     7801 gaacaattat ccaaaaatca ataatattta atttttcaca agcggttgtt tttgcaaaat
     7861 attttgtaaa ttcgaccgct tgcctgtttt taaatgacaa aatacgacga taaaattcag
     7921 catgcattta gcgaaaatgg gttgctcagc caaaatatca gtggatttcg accacgccaa
     7981 gcacagcttg aaatggctca agtggtcgca aaagcagtaa aatttgcgac acctgttgtg
     8041 gtagaggcgg gaaccggcac aggcaaaacc tttgcttatc ttgtgccggc actactttca
     8101 ggcaagaaaa ctattctttc gacaggttct aaaaacctgc aagatcaact ttttaatcga
     8161 gatcttccca ctattcaaaa agccctgaaa tacaaaggca aaattgcgtt actcaaaggg
     8221 cgtgcgaact atttgtgttt agagcgttta gatcaagtga ccgcaatggg cgtacttggg
     8281 gataaatccg ttcttaccga tttaaaaaaa gtcagtaaat ggcaaaatgc cactaaaaca
     8341 ggggatttaa gtgaatgtat taccattgcc gaagatagcc cgattctacc gcagttagtt
     8401 agcaccaccg agagttgctt aggctcggac tgcccgcatt ttaaagattg ttatgtggta
     8461 caagcccgcc gtaaagcgat ggaggcagac gtagtggtgg taaaccatca cctattctgt
     8521 gctgatatgg cagtaaaaaa gaccggtttt ggtgagttga tccctgaagc tgagctggtg
     8581 atctttgatg aagctcacca actgccggat attgccagcc aatattttgg gcaatcgctt
     8641 tcttcccgcc agttatttga tatttgtaag gataccaata ttgtttaccg cactgaactc
     8701 aaagatctgt ctcaattagg caaagcggct gatcacttga cgaaagtagt gcaagatttc
     8761 cgattattga tgggcggaga agggcagatt cgaggcaatt tgcgagagtt atttcgtgat
     8821 aaaaaagtgg tcgatggctt ggtaaaagtg gctgaaaata ttgatttttt aagtgaagtg
     8881 gcgaaaaaat cgctggggcg gtcggaaacg ttggataaaa ttttcgagcg gttagcagaa
     8941 gttaaggttc aacttaaaaa attaatggat actagtgtga caggctattg ttactggtat
     9001 gaggcgaatg gacgatcctt tgggctgcat attacgccgc ttatggtggc agataaattt
     9061 ggtgaacagt tgaagaacca gcaaattggt tgggtgttta cctcagccac actcgaagtt
     9121 gggggcaaat tcgatcattt ttgtcaacgg ctgggcatcg aaaatgcgga gcagttggtg
     9181 ttacaaagtc cgtttgatta ccaacatcaa tctttactct gtgtgccccg ctacttgccg
     9241 gataccaata aatccaatac tttaactgct ctcgggcaat tattaaaacc ggtgattgag
     9301 gcgaataaag ggcgttgttt cttgctttgt acttcctatt atatgatgcg aggtttagcc
     9361 gactttttac gcgaacacag ttcgctgaat gtgttattgc aaggcgaaac cagcaaaagc
     9421 cgtttattgg aaaaatttgt gaaagaagat cacagcgtgt tggtggcgac ccaaagtttt
     9481 tgggagggga ttgatgtgcg aggcgatgat ttatcgttgg tgattattga taaattgccg
     9541 ttcacttcac cggacgaacc attactcaaa gcccgaatgg aagattgtaa gatacagggc
     9601 ggtgatccgt ttaacgatat tcaaattccc gaagcggtga ttaccctcaa acaaggtgta
     9661 gggcgtttaa ttcgtgatgt gaccgataga ggtgtagtga ttatttgtga ttcccgtttg
     9721 gtgatgcgaa attatgggca aactttcttg aaaagtttac cgccgtctag acgcactaga
     9781 gatttgggga atgttattca atttttgaaa caaaaatagg gttacaagcg gtcagatttc
     9841 tgcagatttt tgcaaaaaaa cggataaaaa taaccgcttg ttgaggagtt atatgttgtc
     9901 tacattgctg cgtatggttg cttctttggc attgttattt gtgatgcttt atttaggcaa
     9961 gttaatcgtt tatctattcc ccatcgggat cccggatagt attttaggta tgcttatctt
    10021 attcggctgc ttggttatcg gcattgttaa agtggaatgg gtgataccga gcgcgaaacc
    10081 gctcacacgt tatataacac tatttttcct tccgatttgt gtcgaactca tcgaacatct
    10141 tgagctgtta cgccaaaatc tgaacacttt tgtattagca acggcattaa gcaccttagt
    10201 ttctgtggtc gcgattggcg tgtttgcaca gtggattttt cattacaaga gagggtaaaa
    10261 tgagctatct ttacactgtg ttgacggtaa tcatgtttat tgccggtttg aaaatcagcc
    10321 aaaaactcaa gagcagtttt ctgaaccctt tcttaattgc actcgctttg ctaattggtg
    10381 tattactgat ttttaatatc ccttaccaag actattatca aggtaattcc cccttgaata
    10441 acttacttgg cgtttctgtt gtggcacttg cttacccttt ttatgaacaa ttaccccata
    10501 tccgtaaaaa atggcgacag attgtcgtga ttttgctgtt tagtaccatc attacgatga
    10561 taaccgggct tgggttttca ttattatttg gggcaagcca agagatctta gccgccattt
    10621 tgcctaaatc ggtatcaatg ccaatggcaa ttattattac gaatgaaatt aatggaaatg
    10681 ttgcaatcgc agcggttggg gtgatgatcg caggcttagt gggatctatt ttaggcttta
    10741 cgcttctgcg cctggcaaaa gtaagaaatg tgatggtgat tgggttaagt atgggagctg
    10801 cttcccacgc attaggcacg gcaagatgct tagagcatag tatgaaagcc ggtagctata
    10861 gttctatcgc attagtggct tgtggtgtgc tgtcttctat atttgcacct ttaatattta
    10921 acgttatcgt cacgttatgt tactaaaaac agtgaaatct caaaccttat ttggtaactg
    10981 gctgaaatca aggtacaata ccgtgatttt tcaatgaaaa tagagaatta tgcaagaaca
    11041 aataacacaa gaaaaagatc tttctacgct taaaaccttt aagcgtttat ggccgactat
    11101 cagcccgttt aaagcgggtc tgattacagg tgctgtcgct ttagtattaa atgcgttagt
    11161 ggattctagt cttatctatc tcctcaaacc cttgctagat gaaggctttg ggcaagctaa
    11221 taatgatttt ctgaaattga tggcggtgct tgtagtagtt tttattttag tgcgcggtat
    11281 taccaattat attgccagtt actgtttagc ttgggtgtca ggcaaggtgg taatgacatt
    11341 acgccgccat atttttcaac atttaatgta tatgccggta agctattttg ataacaaccc
    11401 gaccgggcgt ttgctctcac gagtgactta cgataccgaa atggtggcga gttctgcttc
    11461 tcattcttta gtgactattg tgcgtgaagg ggcttatctg ctgtcattgt ttgtggtgat
    11521 gatctatacc agttggcagc tttctattgt gttatttgtg atggctccta tcattggggt
    11581 actgatcagt ttcgtttcaa aatattttcg tcaattaagt cgcaatatcc aaagttcaat
    11641 gggggagctt actgtcacga cagaacaaat gcttaaaggg cataaggttg tactttcctt
    11701 tggtgggcag gaaattgaga aagcacgttt tgatcgagtc agtaatgata tgcgtcgtaa
    11761 agggatgaaa gtcgtttctg ctgatggcat ttctgatggt gtggtacagc ttatcgcctc
    11821 ttttgcgtta tctgctgtgc tttatgttgc gaccttcccc gaggtgatga gtgagaattt
    11881 aaccgcaggt tcattcacag tagtgttttc ttcgatgatg gcaatgttac gtcctctaaa
    11941 atcactcacc aatgtgaact cgcaattcca acgtggaatg gcagcctgcc aaacgttatt
    12001 tgaattttta gatttaaaaa ctgaaaaaga tcacggctta atctccgttg ctaaagctga
    12061 aggcaatgtg gaatttaaaa aggtaagctt tagttatgaa ggtaaagagg aaaaagcact
    12121 cgataatatt tctttcacca ttcctaaagg caaaacagta gcattggttg ggcgttccgg
    12181 ctcgggtaaa tcaactattg ccaatttgct tacccgtttt tatgatgtga ctgacggtca
    12241 aattttgctt gatggaatta atattcaaga ttaccgctta aaaaacttaa gagaacaatg
    12301 ctcggtggtt tctcaacaag ttcatttgtt taacgatact attgctaata atattgctta
    12361 tgctgcaatc gacaaataca gccgagaaga aattattgct gctgcaaaag ctgcacacgc
    12421 aatggaattt atcgaaaaat tggaaaatgg cttagacact gtaattggtg aaaatggcgc
    12481 aagtctttcc ggtgggcaac gccaacgctt ggcaatcgct cgtgcattac tgcgaaattc
    12541 tcctgtgctt gtgctggatg aagctacctc tgcattagat acggagtcag aaagagcgat
    12601 ccaatctgca cttgaagtgc tacagaaaga tcgtacggtg ttggtcatag cacaccggtt
    12661 atcaacgatt gaaaaagccg atgaaatttt ggtagtcgat cacggaaata ttattgagcg
    12721 tggcagccat acggaattgt tagcacaagg tggggcttat aaacaactcc atagtttaca
    12781 attcggagcc taatttgtag gggagaaata tgtttctccc caattaatga taaaaacagg
    12841 gcgaaaaata tttcgtccct acaataattt atttggatac tatgaaaaaa ctgacaaaac
    12901 ttttatctct tagtgcttta ctggtcttaa ccgcctgtaa ttcggtacta aatgcctcaa
    12961 cagaggtgca acaaccaaca gtacaaattc cgcatgacga tccacaatgg cagcagcatt
    13021 tagcgaaact gagccaaatt caaggctata ctgcaacggg gcaatttggc tatatttcgc
    13081 ctgaagagcg tttttcttcc catttcaact ggcgttatac taatccaatc caatttgggt
    13141 tagagctttc ctcgaatctc tcgactaaat cactgaaatt agagcgtaat aaacgaggct
    13201 taacggtttc ggacaatgag ggtaatagcc gttctgaccg tgatgttgat gcgttaatgc
    13261 aagagattat cggtgtttcg tttcctatcg atcagtttgc ctattgggta aaaggcttgc
    13321 ctgaaaaaga tggcaactat attgtgaatg aaaaacgcca gctgtcgcaa tttagttatc
    13381 cgcttaatgg cacgctttgg aaagcaagct atgtggagta tcacgaagat cgtgtaccaa
    13441 acttaccgaa gctgattgtg cttgaaaacg gcactcaaac gttgaagatc cgcattgaaa
    13501 aatgggcgta ttaatgaaga aagtcacctt acccagcccg gcaaaattaa acctgttttt
    13561 atacattacc aataagcggg cagacggtta tcacgagcta caaacgctgt ttcagttttt
    13621 agattttggt gatgaaattg agattgagat cacagaaagt tcagaggttg aattaattaa
    13681 tccggtagaa ggggtggcaa ctgaggagaa tctcatttac cgagcagcaa aacttttgca
    13741 aaaaacgacc gcttgtaggc aaggctgccg tattgggatt aaaaaaaacc taccgatggg
    13801 cggaggtgtt ggtggtggat cttctaatgc tgcaacagta ttagtcggtt taaatcactt
    13861 gtggaatacc gagctttcat tggaaaaatt ggcagaatta ggcttaagtt taggggctga
    13921 tgtgccgata tttgttagag gtatggctgc ttttgccgaa ggcgtggggg aggtgttaac
    13981 tccttgtgat ccacaagaaa aatggtatgt agtgctaaaa ccagaagtgt cgatttcaac
    14041 cgcagcggtg tttaccgacc cgaatttacc tagaaatacg ccaaaacgcc ccttagccga
    14101 attattatct ttaccgtggg aaaacgattg cgaaaaagtt gtacgagaaa agtattctgc
    14161 ggttgaagat cttatacaag aattgttaca atatgctcag tttaggctaa caggcacagg
    14221 tgcttgtatt ttcgccgaat ttgaaacaaa agcagaagcc gagcaggttt ttgcacataa
    14281 accggaagcc gtcttcggtt ttgtggcgaa agggcaaaat atttcgccat tacatcaaat
    14341 gttattccaa atctaaccat cttaacctta aggatcaatc catgacagat attaaactgt
    14401 ttgcgggcaa cgcaactccg gaacttgcta atcgtattgc aaaacatctt tatacctcat
    14461 taggtaatgc aacagttggt cgttttagcg atggtgaaat tcaagtacaa atcaatgaaa
    14521 atgtgcgtgg aggcgatatt tttattgtgc aatccacttg tgcaccaact aatgataatt
    14581 taatggaatt gatcgtgatg gtagatgcgc ttcgccgtgc atcagctggt cgtattaccg
    14641 cagtgattcc gtatttcggt tatgcacgcc aagacagacg cgtgcgttcg gcccgtgtgc
    14701 caattaccgc aaaagtgatt gctgatttct tatccagcgt aggggtagat agagtcttaa
    14761 cctgcgactt acacgcagag caaattcaag gtttctttga tgtgccggta gataacgtat
    14821 ttggttcccc tgtgttaatc gatgatattt tgaaaaaatc cgatttggtc aaccctatcg
    14881 tggtttctcc agatattggt ggggttgttc gtgcaagagc ggtggcaaaa ttattaaatg
    14941 ataccgatat ggcaattatt gataaacgtc gtccaaaagc gaatgtatca caggtaatgc
    15001 acattatcgg tgatgtggct gatcgtgact gtattttagt tgatgatatg atcgatactg
    15061 gtggtacttt agtcaaagcg gctgaagcgc tgaaagaacg tggcgcccgt cgtgtctttg
    15121 cttatgccac acacgcggta ttttcaggca cagcagctca aaatttagcg aatcctgctt
    15181 tagatgaaat tgtagtaact gatactattc cactgagtaa tgagattaaa gcattaaata
    15241 aagtacgtgt tttaacatta tccggtatgt tagcagaagc aattcgccgt attagcaatg
    15301 aagaatccat ttcagcaatg tttgatgcct aatttatgag aaaaaaccca gtcgaaagat
    15361 tgggtttttc atatctgttc actactatat ttagtttaag ataatttgta tgtattccct
    15421 gattcgtaaa ttactttttg cactagatac ggaaaaagca catcaattct ctattcaggc
    15481 acttgcttta gccggaaaat cgcctttttc gattctgcca acacccgata atccgaccca
    15541 agtaatggga ctcacgttta aaaatccaat agggcttgca gccggagcgg ataaaaacgg
    15601 tgaagcgata gatggttttg ggaaattagg atttgggttt attgaggttg ggactattac
    15661 tcctctcgct caagacggaa accctaaacc gaggcaattt cgtattgtag atgcagaggg
    15721 aattattaac cgcaatggat ttaataataa aggcgttgat gttttagtag aaaatgtaaa
    15781 aaatgcaacc tatagtggta ttttaggtat taatattgga aaaaatgcgg ttacaccaat
    15841 tgagcatagc tttgatgact accaaatttg tttgcgtaag gtttaccaat atgctgatta
    15901 tgtggcggtg aatatttcat cacccaatac caaaaatttg cgcagtttac aatatggtga
    15961 ggcattagat gatttgctgc gaaacctcaa aaatgagcag gcacaacttt cgcaaaaaat
    16021 ggggaaatat cgaccgcttg ttttaaagat tgcgcctgat ttaaccgatg aagaaatcgt
    16081 ttctgtagcc gatagtctta ttcgttacca aattgatggg gtgattgccg gcaatactac
    16141 cttgtctcgt gaaagtattg ccggattacc gtattcggaa caactgggtg gattaagtgg
    16201 caagccatta cactcattaa gtacaaggtt aatttcaaaa ttatctgccg agttgggcga
    16261 gagaatacca attataggtt gtgggggagt ccattcgtta gagagtgggc aggagaaagt
    16321 gaatgcaggt gctagtttat tgcaagtcta ttccgcaatg atttaccaag gaccatcatt
    16381 aattcaaacc ttagccaaac accttcagct gaacagttag ggatagattt ctagttaaaa
    16441 atgattggtt aggtttattt ctgtcatctt agtggaatag atggcggtat tagctatcta
    16501 tcaaaaatag acaaaattgc ctat
//
LOCUS       DANLXS010000036        16416 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.036, whole genome shotgun sequence.
ACCESSION   DANLXS010000036 DANLXS010000000
VERSION     DANLXS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16416
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.036"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(84..2273)
                     /gene="yccS"
                     /locus_tag="PXD71_000879"
     CDS             complement(84..2273)
                     /gene="yccS"
                     /locus_tag="PXD71_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDL6291025.1"
                     /translation="MNKFLRYLQHSWLSENVIKTLPMFISLNIAAVSIWQLGISDFAM
                     PLMLGIIAGGLVDLDNSLIGRAKNLIISLVAFSLSSIGASVSLHLGWLFVPVAVVCTF
                     LLVMLGAIGQRYSTIAFGTLIVAVYNSLAYTPEVLWYHNVVMILLGTLLYGLVGILVY
                     LIFPNRTVQENLASAYLALSHYLLAKSAYFDPDDDDLAAKQLALAKANSEVMAAFDKT
                     RVALFYRLSRQHKQQRTQRMLRYYFTAQDILERASSSHYQYHELFQQLSNSDLMFRFQ
                     RVLELQAAACQKIAMSLRHREVYEHGVRGEKALQGLLNSLNYHQERGLKSAYRWRAIA
                     ENLGNIERQLAQIEQGSSSGEHIEPLSKNFTKSHRLIAENITGLSNISQAIKAHLTFE
                     SQLFRHAVRLSIVVLLCSAIVPILGFDSKGYWILLTAIFVCQPNYTATKTRLIQRVIG
                     TIFGVLVGGSFELFNVALSLEAQLGLIVLTGSLYTFFRLVHYGFSTFYITVLVMISLD
                     IAGVSLESGILMRMLDTLLGTAIAWLAVAFLWPDWKYLNLQRNLHNGLKASGQYLRHI
                     IAQLQFGYNEQLPYRIARRDVHNHLSALSNAITNMYSEPQKYANDLTFAPKLLGIAYT
                     LLGYISTLGAYRIASREINQQVDFSAMFFSHAREVANIIGQIASESRSFAKLSEKLTA
                     IDQDLTQFEQENQQKQNDLALVLIQQLRLIVQLLPQLNSILQLKSVR"
     gene            complement(2317..2775)
                     /locus_tag="PXD71_000880"
     CDS             complement(2317..2775)
                     /locus_tag="PXD71_000880"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL6291026.1"
                     /translation="MKTTVRTIAKQKQIALVAHDSCKQDLIRWTQTHQAQLASHKLYA
                     TGTTGHLLARETGLEITSLLSGPMGGDQQLGGLIAEKKIDLLVFFWDPMNAAPHDPDV
                     KALMRIATVWNIPVAINETTANFLLNADLFNQAISVNVPDYDGYLAERLG"
     gene            2901..4175
                     /gene="thrC"
                     /locus_tag="PXD71_000881"
     CDS             2901..4175
                     /gene="thrC"
                     /locus_tag="PXD71_000881"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDL6291027.1"
                     /translation="MNLYNIKHPEEQVNFAQAVRQGLGKNQGLFFPEVLPKLDSIDEL
                     LDLPLVKRSQKILSALIGEELPQETLNAMVKNAFTFPAPLAKVNDDIYALELFHGPTL
                     AFKDFGGRFMAQALANVRGDGKITILTATSGDTGAAVAHAFYGLENINVVILYPKGKI
                     SPLQEKLFCTLGGNIRTVAIESDFDACQALVKQAFDDAELRQAIGLNSANSINISRLL
                     AQICYYFEAVAQLPKEKRDNVVVSVPSGNFGNLTAGLIAKTLGLPIKRFIASTNANDT
                     VPRYLQSGKWEPNATVATLSNAMDVSRPNNWPRVEELFKRNGWSLSELGSAALNDAET
                     EAALKAQYAEGYLCEPHGAIAYQSLKDQLQQGETGIFLCTAHPAKFKESVEHILAIEL
                     PLPEALDKHNKLPLLSDEMAADFATLRAYLLK"
     gene            complement(4254..4988)
                     /locus_tag="PXD71_000882"
     CDS             complement(4254..4988)
                     /locus_tag="PXD71_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL6291028.1"
                     /translation="MSFKDMTGQKVPSVTFRTRQNDAWVDVTTDELFNNKTVVVFSLP
                     GAFTPTCSSTHLPRYNELACEFKALGVDDIICMSVNDTFVMNAWKADQESENITVIPD
                     GNGEFTEGMGLLVDKNDLGFGKRSWRYSMLVKNGIVEKMFIEPQEPGDPFKVSDADTM
                     IKYLSPNWTAKESVSIITKPGCLFCAKAKALLKEQGYTFEEIVLGRDASTISVRAITG
                     KTSVPQVFIGGKYIGGSDELAAYFAK"
     gene            5103..5987
                     /gene="oxyR"
                     /locus_tag="PXD71_000883"
     CDS             5103..5987
                     /gene="oxyR"
                     /locus_tag="PXD71_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDL6291029.1"
                     /translation="MNIRDLEYLIALAEHKHFRRAAESCNVSQPTLSGQIRKLEGELG
                     TILLERTSRKVLFTQAGLTLVEQAKVVLREVKILKEMASNQGKEMSGPLHVGVIPTLG
                     PYISPIIVPALKAEFADLELYIYELQTNQLLDQLESGQLDCGIISFVKESESFIEVPI
                     FNEEMYLVVPEQHEWAALKKFDMNLLKNRELLFLDSGHCLRTNTLDYCLSIGAKENPH
                     FKATNLETLRNMVAANVGLALIPKLAAKPIKGVHYIPLENPKPFRAVGMVYRPGSPLR
                     ARYERLAQAITKIMQSEA"
     gene            5990..6622
                     /gene="fabR"
                     /locus_tag="PXD71_000884"
     CDS             5990..6622
                     /gene="fabR"
                     /locus_tag="PXD71_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDL6291030.1"
                     /translation="MANGMSVRAIQKEKTRRALVEAAFNQLSSEKNFSNLSLREVARE
                     AGVAPTSFYRHFKDMDELGLAMVDESGLLLRQLMRQARKRIANGGSVVVVSVETFFEL
                     IADRPNVFQLLLRESSGTSQAFRTAASREIQHFVAELADYIMSKDNHSNRELAYIQSE
                     GLVTLVFTAGSHALDMNNQEREKLKQRVIMQLRMLIRGSTYYANKQASST"
     gene            complement(6649..6948)
                     /locus_tag="PXD71_000885"
     CDS             complement(6649..6948)
                     /locus_tag="PXD71_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HDL6291031.1"
                     /translation="MKKADIQKMQDCYNQWVELLPELEKGIEQWKRASALLEPLSRFY
                     ASPEWRELHDSFDEQLDTKGNYSILSEDALWNALSEHHQLALEWLKLSTAYITKE"
     gene            complement(7037..10852)
                     /locus_tag="PXD71_000886"
     CDS             complement(7037..10852)
                     /locus_tag="PXD71_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL6291032.1"
                     /translation="MKGSGLATVGAPETGDNGQTIYTVDVAKAGAPTVTRGNVADAIN
                     NSEKTSSVVAGSKAVTVKAGKEDDKGNTEYTVDVATDKSIGRDNDGNMTVNTDNVTIV
                     KDPTTGEVKANTTTLNNSPEGKVTEPTGDDAKKLVTAGDIANAINNSGFNVTAGDGVD
                     GGETKGKKTQLIKPSETVTFDAGKNMTLTQADGKFTYTTKDNVAFNSIDMSNGKPDTT
                     GSITNLKSGVGGTFADKAAPTDAERKAIADNINNATGDTLNNAVNVGDVQAAMKAATT
                     KVEGDKGVTVTSKTNDDGSTTYTVEAKTDGTTIKVNDKGEITANTSELTNNPDGKVEE
                     PTEPNALVTAKTVADAINNAGFNIKANGDEKSLVKTGDTVQFLNGKNIEITRDGNNIT
                     VSTAKNVNFDSVQFGNEGPKITNNGGNINVGDKDGNAVKVTNVAAGDVNADSKDAVNG
                     SQLYTFAMASREEVKSTDKSVTVNTTKNADGANVFDLSVNTDDVTIVKDPTTGAIKAN
                     TTALNDANNDGRIDEPTADDAKKLVTAGDITNAINNSGFTLKTSAVEGGEKLSGGDEL
                     INPGKAVEMVAGKNLTVKQEADGKVIYATKDDVKFSSVTSNTVTVPTDEADPANNPIT
                     INKDGINAGNKAISNVASNLIPVTADDKVQPADNNPTNLADKLSNAATLGDVLNAGWN
                     LQGNGKAVDTVVHNDTVDFINGKGTTVTVENKDGKNTIKVDSPIEFVNQDPTDSSTPS
                     NTAKFTGEAPVQLGNVASSVRNEDGSTPEGKDRAEAIKNAEGDKLNNVVNLGDLQAAT
                     NAATTKVGGNRGVTITPSTNADGSTTYNVEAKTDGTTIKVDNEGNITANTSELGNNED
                     GTVKAPTQPNALLIAQTVADAVNNAGFNIKSAGNKAAGDQAATKLVKTGEEVVFEAGD
                     NLTVKRDGNQFTFATAKDVSFNSVQFSENGPKITNDGDNIKVGDKDGKPTKITNVADG
                     DISPVSTDVINGKQLNNYAKVNGNNIGTDEDGSINIVNGNGTTITSDKAGEVKVNVNI
                     TDLTVADNGKINVQDPNGTGSRFVNATTVANAVNNVSWNVDSKAVGTGAVEGDKAPAK
                     VKAGSTVSVNAGNNIKVTRKGSDVTVAVSDTPEFTSKTGDTLVNNNGVTINNGSAGKA
                     VSLTKDGLNNGGNRITNVKAGEADTDAVNVGQLKGAVNHLNNKIHRNNREARAGIAGS
                     NAAAALPQVYIPGKSMVAAAGGTFKGENALAVGYSRSSDNGKLILKLQGNANSRGDFG
                     GGVGVGYQW"
     gene            complement(10815..11300)
                     /locus_tag="PXD71_000887"
     CDS             complement(10815..11300)
                     /locus_tag="PXD71_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291033.1"
                     /translation="MDTGSITVDNTTGAVTTPAEEDKVATTKTVSEAIQKAGWNAKSG
                     GNKADGDQEAAELINPGEKVIFAAGDNLKVKRVGTIFTYETAKDVKFDSVTFGDNGPK
                     ITNKDGNVNIAGNDGNPTKITGVKAGEADTDAVNVSQLKQAAASQNRSERFRFSNCWC
                     T"
     gene            complement(11309..13468)
                     /locus_tag="PXD71_000888"
     CDS             complement(11309..13468)
                     /locus_tag="PXD71_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6291034.1"
                     /translation="MGARATSANAIALGTDTAATGNRATAFGAGALATGNRSTVMGWR
                     SAASGTRSFAMGSGAMGDKSVANATYSLALGSSANANATSTATRGTAIGYNATVSGVN
                     ATALGVSAVSSNQSTVAIGDSAKASAGFAIALGSGANVALNNSVALGSNSTVSNAVAV
                     PSATVNGITYSGFAANTTAAGNVVSVGSNTIKRQIQNVAAGQISATSTDAINSSQLYM
                     AMNATGNLANSTKNILGGNATVKPDGSVTYTNIGGTNKNTIEEALKAVKTEVVAGSNV
                     NITNATGANGQTIYTVNAYNTTANSSSPDYITVTGKAATAANTTNYEIGLTKKAIDDF
                     TKDTQATVVSNDGTVTVKSTERNANGTVIYDLSVNIPAQASQIQYFSVNSTVPENQAN
                     DGAKSRNSIAIGPNATATGGEQAAVALGTNSNANGNGALSLGVATVSKGIQATAVGHS
                     ANATANGTTALGRQTNATAGDATAVGSNANATAEKASAFGVAANASANASLAVGANSI
                     ASAQSAVAVGTRANATAQFATALGMGAQATLNSSVALGSESVVRAATPTENATVGGIT
                     YNGFAGVNKDTNYVVSVGSAGKERQIQNVAAGQISATSTDAINGSQLYMAMNATSNLA
                     NSTANNFGGGSVVNPDGSVTQPKYNVTNAAGTQYGNTATNVGDAITNLNNYVNQGFNI
                     KDNAGETKGTVTPNESVQFVNGKGTVSNVTQEADGVK"
     gene            complement(13697..13897)
                     /locus_tag="PXD71_000889"
     CDS             complement(13697..13897)
                     /locus_tag="PXD71_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HDL6291035.1"
                     /translation="MNQIFKVIWNQATQSWVAVSELQRAKGKTKSVSSASKATLALLA
                     GASAVLGANDAVAVVSYRFNNK"
     gene            14261..15415
                     /locus_tag="PXD71_000890"
     CDS             14261..15415
                     /locus_tag="PXD71_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HDL6291036.1"
                     /translation="MNQFFPLIFSLTLFSGVTLALEKAPRTMPTEGINKPLLGQILFF
                     DNMLSHNGSQNCSSCHNPDYAFIDNRTTPAKGVVSQGDTPTKFGKRNSPTMLYAKYSP
                     EFHFDEQSQEYIGGQFWDGRAKNLAEQAGMPPIDPNEMGMPNKLEVAKRLYNVPMYQR
                     FLGQFYGEKVWESYESVYAAMEDAIATFQQENKLLAPFDSKYDLFLKGEVQLTALEEK
                     GRALFFNKAQTNCSSCHQLQSAANHQEETFSNYHYYNVGVPGNPDLIAVNGFAPDFVD
                     LGLFENPNVKGDETQKGKFKTPTLRNVAVTALYMHNGVFKELKTVLLFLDSYNNPERK
                     INPETGTAWQQAEFEPTIAHQKLKAKPLSDEEIDALEAFLKTLTDERYRK"
     gene            complement(15463..>16416)
                     /locus_tag="PXD71_000891"
     CDS             complement(15463..>16416)
                     /locus_tag="PXD71_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL6291037.1"
                     /translation="KSNVALLVVENEAWLTQNQLAELFDTSVQNISEHIKNILKDKEL
                     DENSVIKDYLITAQDGKNYQVKHYSLEMILAIGFRVRSPRAVQFRKWANSTLRTYLEK
                     GFLIDSERLKNPQGRLDYFDELLEQIREIRASELRFYQKVRELFKLSSDYDKTDKNTQ
                     MFFAETQNKLIYAITKHTAAELICQRADATKMNMGLTSWKGKVVRKGDIVIAKNYLDN
                     NELDSLNRLVMIFLESAELRVKNNQDLTLGFWRRNVDMLIEFNGFSVLGNGGTITHKQ
                     MESFVREQYEKFDIQRKCLKQKEADWEDLQALEKLESELTR"
BASE COUNT         4667 a         3715 c         3020 g         5014 t
ORIGIN      
        1 catcaacaaa aaattaacaa aaccataata tttttattga ttttgtgaag catgtcacat
       61 tttatgattt aattataggc tcactacctt acactcttga gctgtaatat actatttaac
      121 tgcggtaata actgcacgat cagtctgagt tgttgtatca acactaaggc gaggtcattt
      181 tgcttttgct gattttcttg ctcaaattgg gtgaggtctt ggtcgatagc ggtcaatttt
      241 tccgataatt ttgcaaatga acggctctct gaggcaattt gcccgatgat gtttgccacc
      301 tctctggcgt gtgagaagaa cattgcggaa aaatccactt gttgattaat ctctcggcta
      361 gcaatacggt aagcccctaa ggtggaaata taacccagca aggtgtaagc aatacctaac
      421 aattttgggg cgaaggttaa atcgttcgca tatttttgtg gttcgctgta catattggta
      481 atcgcattac tcagtgccga taaatggtta tgcacatcac ggcgggcaat acggtaaggc
      541 agttgctcgt tataaccaaa ctgcaactgg gcgataatat ggcgtaaata ttgcccactg
      601 gctttcaagc cgttatgtaa gttacgttgt aaatttaaat acttccaatc cggccataag
      661 aatgccactg ccagccacgc aatcgctgta cctagtaagg tatcaagcat tcgcattaag
      721 atgccgcttt caaggctcac gcctgcaata tcaaggctta tcattaccaa cacagtgata
      781 taaaaggttg aaaagccata atgtaccaag cggaaaaagg tataaagcga accggtcagt
      841 acgattagcc caagctgggc ttcgaggctg agcgccacgt taaacagttc aaaagagcca
      901 ccaaccagca cgccgaaaat tgtgccgatc acacgctgaa tcagtcgggt cttagtagca
      961 gtgtaattcg gttggcagac aaaaatggct gtgagtaaaa tccagtagcc tttgctatca
     1021 aagcctaaaa tcggcacaat cgcactgcac aacagcacca ctatcgacaa ccgaacggcg
     1081 tgtcgaaaca gttgggattc aaaggttaaa tgggctttaa ttgcttggct gatattgctt
     1141 aagccggtaa tattttcggc gatcaagcgg tgagattttg tgaaattttt acttaacggt
     1201 tcaatatgct caccactgct tgagccttgt tcaatctggg ctagttgccg ctcaatattg
     1261 cctaaatttt ctgcaatagc acgccagcga taagcacttt tcaagccccg ttcttgatga
     1321 taattcaatg aattaagcaa gccctgcaag gctttttcgc cacgaacgcc gtgttcatat
     1381 acttctctat ggcgtaacga cattgcaatt ttttgacacg ccgcagcctg taattccagc
     1441 acccgttgaa agcggaacat taaatcactg ttacttaatt gttggaacag ttcgtgatat
     1501 tgataatggc tggaacttgc ccgctctaaa atatcttgag cggtgaaata gtaacgcaac
     1561 attcgctgtg tccgttgctg cttgtgctgg cggcttaggc ggtagaatag ggcaactctg
     1621 gttttatcaa aggcagccat cacctcacta ttggctttcg ccaaggctag ttgtttggca
     1681 gctaaatcgt catcatcagg gtcaaaatag gcagatttcg ccagtaaata atggctcaac
     1741 gcaaggtagg cggacgctaa attctcctgc acggttcgat tcgggaaaat caggtacacc
     1801 aaaataccga ccaaaccata gagtaaggtg ccaagtaaga tcatcaccac attgtgatac
     1861 cacaatactt cgggcgtata agccaggctg ttataaaccg ccacaatcag cgtgccaaaa
     1921 gcaatggtac tatagcgttg cccgattgcc cctaacatta ccagcaaaaa ggtgcaaacc
     1981 accgccaccg gcacaaacag ccagcccaaa tggagcgaca cactcgcccc aatcgaggaa
     2041 agcgagaacg ccactaggct gataatcaga tttttcgctc gtccgattag gctgttgtct
     2101 aaatccacca aaccgccggc gataatgcct aacatcaacg gcatcgcaaa atcagaaata
     2161 ccgagctgcc aaatgctgac cgccgcaatg tttagcgaaa taaacatcgg cagggtttta
     2221 attacatttt ccgaaagcca actgtgttgt agatatctta aaaatttatt catatttgct
     2281 catttcgggc aggcacagga cctgccccta cctacactac cccaaacgtt ctgccaaata
     2341 gccatcataa tccggcacat tcacgctgat tgcttgattg aacaaatccg catttaacag
     2401 aaaattagcg gtggtttcgt taatcgccac cggaatattc caaacggtcg caatccgcat
     2461 cagggctttc acatcaggat cgtgcggagc ggcattcatc ggatcccaaa agaacaccag
     2521 cagatcaatt ttcttttctg cgatcagccc gcctaattgc tgatcgcccc ccatcgggcc
     2581 gcttaacaat gaagtaattt caagccctgt ttctctggcg agcaaatgcc cggtagtgcc
     2641 ggtggcatat agcttatgcg aggcaagttg ggcttgatga gtttgtgtcc aacgaattaa
     2701 atcttgttta cagctatcgt gagccactaa ggcaatctgt ttttgttttg cgatggttct
     2761 gacggtggtt ttcattttta gctcctttgc aaaatttttc acaaatcata ccgcttattt
     2821 ttcgcttttg cattgactaa tcacggctta tggctaaaat caggtaaaaa ttcgcatttt
     2881 aaaacaaagg attaaccgca atgaacttat acaacatcaa acaccctgaa gaacaagtca
     2941 atttcgccca agcggtacgc caaggcttag gcaaaaatca agggctattt ttccctgaag
     3001 tgttacctaa attagatagc attgatgaat tattggattt acctttggtt aaacgcagcc
     3061 aaaaaattct ttcagcctta atcggcgaag agttaccgca agaaacctta aatgcaatgg
     3121 taaaaaatgc gttcactttc ccggcaccgc ttgccaaagt caatgatgat atttatgcgt
     3181 tagaattatt tcacggtccg actttagcct tcaaagattt tggtgggcgc tttatggctc
     3241 aagccttagc caatgtacga ggagacggca aaattactat tttaaccgct acctcaggtg
     3301 acaccggtgc ggcggtggct catgctttct atggtttgga aaatatcaac gtggtcattc
     3361 tttacccgaa aggcaaaatc agcccattac aagaaaaatt attctgtacc ttaggcggta
     3421 atattcgcac tgttgcgatt gaatccgact ttgatgcttg ccaagcctta gtgaaacaag
     3481 cctttgatga tgccgagcta cgccaagcga ttggtttaaa ttccgcaaat tccatcaaca
     3541 ttagccgttt actggctcaa atttgctact actttgaagc agttgctcag ttaccaaaag
     3601 aaaaacgaga taacgtagtg gtttcagtac caagcggtaa tttcggtaac ttaaccgccg
     3661 gcttaattgc caaaacatta ggtttaccga ttaaacgttt tattgcctca accaatgcca
     3721 atgacaccgt gccacgctat ttacaatcag gcaaatggga accaaacgca accgttgcaa
     3781 ccctttctaa tgcaatggac gtaagccgcc cgaacaactg gccacgagtt gaagaattat
     3841 ttaaacgcaa tggctggagt ttgtctgaat tgggttctgc cgcattaaac gatgccgaaa
     3901 ccgaagcggc tttgaaagct caatatgcag aaggttatct gtgtgagcct cacggtgcta
     3961 ttgcttacca aagcttgaaa gatcagctac aacaaggcga aaccggtatt ttcttatgca
     4021 ccgctcaccc ggcaaaattc aaagagtcgg tggaacacat tttagcgatt gaattgccgt
     4081 taccggaagc attagataag cacaacaaac tgccactgtt atccgatgaa atggcagcag
     4141 attttgcaac attgcgtgca tatttgttga aataaatatt caataataga aaagggatta
     4201 agtattaaac ttaatccctc tttttatgca ttacaagcgg ttatatttgc ctattatttt
     4261 gcaaaataag cagctaactc atcactaccg ccaatatatt taccgccgat aaatacttgt
     4321 ggcacagagg ttttacctgt aattgctcgt acagaaattg tgcttgcatc acgacctaac
     4381 acaatctcct caaaagtata gccttgctct tttaataatg cttttgcttt tgcacagaac
     4441 aggcaacccg gtttagtgat aatcgaaaca gactctttag ctgtccaatt tggacttaaa
     4501 tatttaatca ttgtatcggc atctgatact ttaaacggat cgcccggttc ttgtggctca
     4561 ataaacattt tttccacaat accgtttttc accagcatcg aataacgcca agaacgttta
     4621 ccaaacccaa ggtcattttt atcaactaat agccccatac cttctgtgaa ctcaccatta
     4681 ccatcaggaa taacagtaat attttcagat tcttgatctg ctttccaagc gttcattaca
     4741 aaggtatcat taactgacat acagatgata tcatctaccc ctaatgcctt aaattcacaa
     4801 gctaattcat tatagcgagg caaatgtgtt gatgaacaag tcggtgtaaa tgctccgggt
     4861 aatgaaaaca caaccaccgt tttgttatta aataactcat cggttgtgac atcaacccaa
     4921 gcatcgtttt gacgtgtacg aaatgttaca ctgggtactt tctgacccgt catatcttta
     4981 aaagacatat taatgttctc ctatagaatt atatattaca aaaatcttag gcattatact
     5041 cacaaatatg gcataattca gcctatcaga acaataggaa aaatttaaat tggaggaatc
     5101 aagtgaatat tcgagattta gaatacctta ttgcacttgc agaacataag catttccgtc
     5161 gtgcagcaga atcttgtaat gttagccaac ctactttaag cgggcaaatt cgcaaattag
     5221 aaggcgaact aggcactatt ctgcttgaaa gaaccagtcg caaagtcctc tttacgcaag
     5281 caggcttaac attagttgaa caagccaagg tagttttacg tgaagtaaaa attctaaaag
     5341 aaatggcaag caaccaaggt aaagagatgt ctggcccgct tcatgttggt gtaatcccaa
     5401 ccttggggcc ttacatttcg ccaattattg tgcctgcatt aaaagcagaa tttgcagatt
     5461 tagagctgta tatttacgaa ttacaaacca accaattatt agatcaactt gaatccggcc
     5521 agcttgattg tggcattatc tcatttgtaa aagagagtga atcttttatt gaagtgccta
     5581 tctttaatga agaaatgtac ttggtagttc cagaacaaca cgaatgggca gcgcttaaaa
     5641 aattcgatat gaatttatta aaaaaccgtg agttgctctt tttagacagt ggccattgtt
     5701 tacgcactaa tacgctagac tattgtctat ccattggagc aaaagaaaat ccacacttta
     5761 aagctaccaa ccttgaaacc ttacgcaata tggtagcagc aaatgttggc ctagcattga
     5821 ttcctaaatt agccgctaaa ccgattaaag gagtacatta tattccactt gaaaacccga
     5881 aaccgtttcg agctgtggga atggtgtatc gaccaggttc accattacgc gcccgctatg
     5941 agcgtttagc tcaagccatt actaaaatta tgcaatccga ggcgtaaaaa tggcaaacgg
     6001 tatgagtgtg cgcgcaattc agaaagaaaa aacacgcaga gcattagtag aagccgcctt
     6061 taatcaatta agctcagaaa aaaatttctc taatctcagt ttgcgtgaag ttgcacgtga
     6121 agctggagtc gcaccgactt cgttttaccg ccactttaaa gatatggacg aactaggctt
     6181 agcaatggtg gacgaatccg ggctactatt acgccaatta atgaggcaag ctcgaaaacg
     6241 aattgcaaat ggcggaagtg ttgtcgtggt ttcagtggaa acattcttcg aactgattgc
     6301 cgaccgccct aacgttttcc agctattact acgcgaaagc tccggtacat cgcaagcgtt
     6361 tagaaccgca gcatcacgcg aaattcaaca ttttgtagct gaattggcgg actatattat
     6421 gagcaaagat aatcacagta accgagaact tgcttatatt caatcggaag gtttagtcac
     6481 gctagtattt actgccggtt ctcatgcttt ggatatgaac aatcaagaac gggaaaaact
     6541 aaagcaacgt gtcattatgc agctaagaat gctgattcgt ggctccacct attatgccaa
     6601 taaacaagct tcatcaacat aaaaaaacag acgcctaagc gtctgttttt attctttcgt
     6661 aatatatgca gtcgatagct ttaaccattc tagtgctaat tgatgatgtt cagataacgc
     6721 attccataac gcatcttctg acaagatact ataattgcct ttagtatcta actgttcatc
     6781 aaaactatca tgtaattcac gccattccgg gctagcataa aaccgactta agggttccaa
     6841 aagtgcagaa gctcgtttcc attgctcaat ccccttttcc aattctggta atagctctac
     6901 ccattggtta taacaatcct gcattttctg aatatctgct tttttcattc tttaccgctt
     6961 acaccataaa ttttagcctc aatataacat aaaaatcccc ttccgaagaa ggggatttca
     7021 actcaattag gctaaattac cactggtatc caacgcctac accgccacca aagtcgcctc
     7081 ggctattcgc attaccttgt aatttcaaga taagcttacc gttatcactt gaacgtgaat
     7141 aacctactgc aagcgcgttt tcacctttaa atgtaccacc tgctgcagcc accatagatt
     7201 tacctggaat gtaaacctgc ggtaacgctg ctgctgcatt tgaaccggca ataccagcac
     7261 gggcttcacg gttattacga tggatcttgt tatttaagtg attaacagca ccttttaact
     7321 gaccaacatt aacagcatcg gtatctgcct cacctgcttt aacattagta atacggttac
     7381 caccattatt taagccatct ttcgttaagc ttaccgcttt tcctgcagag ccattattaa
     7441 tggttacgcc attattgttc accaatgtat caccagtctt gctagtaaat tcaggggtat
     7501 ctgaaacggc tacagttaca tcagaacctt tacgtgtcac tttgatatta ttacctgcat
     7561 tcacgctcac tgtactacct gcttttactt tcgcaggggc tttatcccct tcaaccgcac
     7621 ctgtaccaac tgctttagaa tctacgttcc agcttacgtt gtttacggca tttgctactg
     7681 ttgttgcatt cacaaagcgt gaaccggtac cgttaggatc ttgaacattg attttgccgt
     7741 tatcagcaac ggttaaatct gtgatgttta cgttcacttt cacctcacca gccttatcac
     7801 tggtaatagt tgtaccatta ccatttacga tattgataga accatcttca tcagtaccaa
     7861 tgttattacc attcactttc gcatagttgt tcagctgttt accgttgata acatctgtgc
     7921 ttactgggct aatatcgcca tctgctacat tagtgatctt agttggttta ccgtctttat
     7981 caccgacttt gatgttatca ccatcattag tgattttcgg accattctca ctgaactgta
     8041 cgctattgaa gctcacatct tttgctgtcg caaaagtaaa ttgattacca tcacgtttaa
     8101 cagttaagtt atcacctgct tcaaacacaa cttcttcgcc cgttttcact agcttagtcg
     8161 cagcttggtc accggcagcc ttattacctg cagatttaat attaaagcct gcattattca
     8221 ctgcatctgc aacagtttga gcaatcaata acgcatttgg ctgagttggt gctttcaccg
     8281 taccatcttc gttgttacct aactcactgg tatttgcagt gatgttacct tcattatcta
     8341 ctttaatagt agtaccgtca gttttcgctt caacgttata agtagttgaa ccatcagcat
     8401 tcgtgctagg tgtgatagtt acacctcggt taccgcccac tttcgtagta gctgcattag
     8461 ttgccgcttg taaatcacct aagttcacta cgttgtttag cttgtcgcct tcagcatttt
     8521 taatcgcttc tgcacggtct ttaccctctg gggttgagcc atcttcgtta cgaacactac
     8581 ttgccacatt gcctaattga acaggggctt cacctgtgaa tttagccgta ttgcttggcg
     8641 ttgagctatc agtaggatct tggtttacaa actcaatcgg gctatctact ttaatcgtat
     8701 tcttaccgtc tttgttttca accgtgaccg ttgtaccttt accgttaata aaatcaaccg
     8761 tgtcattatg taccacagta tcaaccgctt tgccgttacc ttgtaaattc cagcctgcat
     8821 ttaatacatc acctaatgtt gctgcattac ttaacttatc agctaagttg gttggattat
     8881 tgtctgctgg ttgaacctta tcatctgcag tcacaggtat cagattagac gcaacattgc
     8941 taatcgcttt attacctgca ttgataccat ctttattgat agtaattgga ttatttgcag
     9001 gatctgcttc atcagttggt acagttacag tgttagaggt tacgctgcta aacttaacat
     9061 catctttagt cgcataaatg actttaccat cagcttcttg cttcacagtt aagtttttac
     9121 ctgcaaccat ttcaactgct ttacccggat taatcagttc atcaccacct gaaagcttct
     9181 ctccaccttc tacagccgac gttttcaacg taaagcctga gttattgatg gcgttggtaa
     9241 tatcacctgc tgtcactaat ttcttagcat catcagcggt tggttcatca attctaccgt
     9301 cattatttgc atcatttaat gccgtagtgt tagccttgat cgcaccggtt gtaggatctt
     9361 ttacaattgt tacatcgtcc gtattcacag aaagatcgaa cacattcgcg ccatcagcat
     9421 tttttgtcgt gttaaccgta actgatttat cagtcgactt cacttcttca cgagatgcca
     9481 tagcgaaagt ataaagctga ctaccattta ctgcatcttt actatctgca tttacgtcac
     9541 cggcagctac attagttact ttgactgcat taccgtcttt gtcaccaaca ttgatattac
     9601 caccattgtt agtaatctta ggaccttcat ttccgaactg tacactatca aaattcacat
     9661 tttttgcagt tgaaacagta atgttattcc catcacgggt aatctcaatg tttttaccat
     9721 ttaagaactg aaccgtatca cctgttttca ccaggctctt ctcgtcaccg tttgctttaa
     9781 tgttaaaacc tgcattgtta attgcatctg caacggtttt agctgttact aatgcatttg
     9841 gttctgtagg ctcttccact ttaccatcag ggttatttgt taactcgctg gtgtttgccg
     9901 tgatttcgcc cttatcgttt accttaatgg ttgtaccatc cgtttttgct tcaacggtat
     9961 aagtggttga gccgtcatcg ttagttttag atgttacagt aacaccttta tcaccttcca
    10021 ctttggtggt tgctgctttc attgctgctt gtacatcacc cacattcaca gcattattca
    10081 acgtatcacc tgttgcgttg ttaatattat ccgcaatcgc tttacgctct gcatctgttg
    10141 gtgctgcttt atctgcaaat gtaccaccca cgccgctctt cagattagtg attgagcctg
    10201 ttgtatctgg tttaccattg ctcatatcaa tgctgttgaa tgcaacatta tctttcgttg
    10261 tgtaagtgaa tttaccgtca gcttgggtaa gtgtcatatt tttacccgca tcaaaggtaa
    10321 ctgtttcact tggcttgatt agctgtgttt tcttaccttt ggtttcacca ccatcaacac
    10381 catcacctgc tgttacatta aagcctgaat tattgattgc attagcaata tcacctgccg
    10441 tcactaattt cttagcatcg tcacctgtag gctcagtaac tttaccttca ggagaattat
    10501 ttaatgttgt ggtattcgct ttaacttcgc ctgttgtcgg atctttcacg atagtaacgt
    10561 tgtcagtatt cactgtcata ttaccgtcat tatcacgacc gatagattta tccgttgcaa
    10621 catctaccgt atattcagta ttgcctttat catcttcctt tccggcttta acagttacgg
    10681 ctttagaacc agctactaca gatgaggttt tctctgaatt attaattgca tcagccacat
    10741 taccacgagt tactgtagga gcacctgctt tcgctacatc taccgtatag attgtttgac
    10801 cattatcacc tgtttcaggt gcaccaacag ttgctaaacc tgaacctttc acttcggttt
    10861 tgtgacgctg ctgcttgttt taactgactt acattcaccg catcagtatc agcttcacct
    10921 gctttcacgc ctgtaatttt ggttggatta ccatcattac ctgcaatatt aacattacca
    10981 tctttattcg tgattttcgg gccattatca ccaaatgtta cgctatcaaa tttcacatct
    11041 ttcgctgttt cgtacgtgaa tatagtgcca acacgtttaa cttttaagtt atcgcctgct
    11101 gcaaaaatca ctttttcgcc cggattaatc aactctgctg cttcttgatc accatcagct
    11161 ttattgccac cagattttgc attccaacct gctttttgaa tcgcttcaga aactgttttt
    11221 gtagttgcaa ctttatcttc ttctgcaggt gtagttactg cacctgttgt gttatcaacc
    11281 gtgatagaac cggtatccac atcaaaggtt actttacgcc atcagcttct tgtgtaacat
    11341 ttgaaactgt acctttaccg tttacaaatt gtacagattc atttggggtc actgtgcctt
    11401 tggtttcacc cgcattatct ttaatattga aaccttggtt tacatagtta tttaagttcg
    11461 taattgcatc gccaacgttt gttgcagtat taccatactg agttccagct gcattcgtta
    11521 cattatattt aggctgagtc acagagccat ccggattcac aacagaaccg ccaccaaaat
    11581 tatttgcagt tgaatttgca aggttactgg ttgcattcat tgccatataa agttgtgaac
    11641 cattaatcgc atcggttgat gtagcagaaa tctgacccgc tgccacattt tggatttgac
    11701 gctctttacc tgcagagcct acagaaacaa catagttagt atctttattt accccggcaa
    11761 agccattata agtaataccc cctaccgttg cattttcagt tggtgttgct gcccttacga
    11821 ctgattcgct acccaaagca acagaactat tcagagtagc ttgagctccc atacctaatg
    11881 ctgtagcaaa ttgagctgtt gcattagcac gtgtaccaac agccactgct gattgtgctg
    11941 aggcaattga atttgcacct acggctaaag aggcatttgc actcgcattt gctgcaacac
    12001 caaaggcaga agctttttca gccgttgcat tagcattaga acctaccgct gtagcatcac
    12061 ctgctgtcgc atttgtttgg cgaccaagtg cggttgtacc attagccgtt gcatttgctg
    12121 aatgaccaac tgccgtagct tgaatacctt ttgaaaccgt agcaacacct aaagataacg
    12181 ctccgttacc atttgcattt gagtttgtgc ctaatgctac tgccgcttgc tctccacctg
    12241 ttgccgttgc attcgggccg atcgcaattg agtttctgct cttcgcgcca tcattagctt
    12301 gattctcagg tacggttgaa ttcacagaga aatattgaat ttggcttgct tgcgcaggaa
    12361 tattgacaga aagatcataa ataaccgtgc catttgcatt tctctctgta ctttttacag
    12421 ttactgtacc atcgtttgaa acaacagtag cttgggtatc tttcgtaaag tcatcaatgg
    12481 ctttcttagt taaaccaatt tcatagttag ttgtattcgc agcagttgca gctttacctg
    12541 ttacagtaat gtaatcaggg ctagaagaat ttgccgtggt gttatacgca tttactgtat
    12601 aaatagtttg cccatttgca ccggttgcat ttgtaatatt aacattgcta cctgcaacaa
    12661 cttctgtttt taccgctttt aatgcttctt caatcgtatt tttatttgtt ccaccaatat
    12721 tagtataggt aacagaacca tcaggcttta ctgtagcatt accacctaaa atatttttag
    12781 ttgaattagc aaggttacct gttgcgttca ttgccatata aagctgtgaa ctattaattg
    12841 catcggttga tgtagcagaa atctgacccg ctgccacatt ttggatttgg cgtttgatcg
    12901 tgtttgagcc aacagaaaca acattccctg ctgccgtagt attagccgca aagccggaat
    12961 aagtaatacc atttaccgtc gcagaaggaa ctgccacagc gttggaaacg gttgaattac
    13021 tccctaaagc aaccgaatta tttagcgcta cattagcacc tgagcctaag gcaatagcaa
    13081 aaccggcact tgctttagca ctatcaccaa ttgcaaccgt agattggttg gaagatactg
    13141 cacttacccc taaagctgtc gcattaacac ctgaaactgt cgcattataa ccaatcgctg
    13201 tacctctagt tgcagtagaa gttgcattag cattagcact tgaacctaaa gctaaagaat
    13261 aagtagcatt tgcaacagac ttatccccca ttgcacctga acccattgca aatgaacgag
    13321 tgccactagc ggcagatctc catcccatta ctgttgaacg gttaccagta gctagagctc
    13381 ctgcaccaaa agctgttgca cggttacctg ttgcagcagt atccgtacct aatgcaatag
    13441 catttgctga agtcgctcta gcccccatac caatagcaat agaaggaact aatgcccaat
    13501 tctgaccagt gttattgaga ctctctacac caatattata gtaatgtaag aagtatcttc
    13561 tggatccact aaatttgttc tatgcgttgc attcgtatct gtaccaattg caatagtaga
    13621 tctgccagtt gagattgaat ccgcaccaat agcaattgcg ttctccgatg atgcagttgc
    13681 ttgtgttgaa atataattac ttgttgttga acctataaga tacaacagcc acagcatcat
    13741 tagctcccaa cacggcacta gctccagcca ataaagccaa tgttgcctta cttgcagaac
    13801 taacagattt cgttttacct ttcgctcgtt gtaattcaga tactgcaacc caactttgtg
    13861 ttgcctgatt ccaaataact ttaaaaattt gattcataat cgaccctata taaaaataaa
    13921 aaagattacg tagaacctat gttcaacata atcttcacca atataaaaaa ccgccaccta
    13981 tttaagtgaa ataacttact tatagactca gaaatttaag tgcgcgtatt ttacgctatt
    14041 tgtaatataa ttcatcttct ggagtgtaaa tttattgtaa ataatacgag gaatctaacc
    14101 gcttgttata acgcaaaaca ccttgactta aatcaagcca ctacctcatt ttgaggggag
    14161 atttatacgc agtgaaattt taaaataagc taaggtttac ggataatcgt tcaacactat
    14221 tgaattatca ctgtagttat cgttaaaaag gtatgctttt atgaaccaat tcttccctct
    14281 aatctttagc ttaactctat tttccggcgt aactttggca ctagaaaaag cacctcgaac
    14341 tatgccgaca gaaggaataa ataagcccct acttggacaa attctattct ttgacaatat
    14401 gctgtcacac aacggttcac aaaactgctc cagctgccat aatccggatt atgcctttat
    14461 tgataaccgc actactccgg caaaaggggt ggtttcacaa ggagatactc caaccaaatt
    14521 cggcaaacgc aactccccga ccatgcttta tgcgaaatac tcgcctgagt tccactttga
    14581 tgagcaaagt caagaatata tcggcgggca gttctgggac ggacgagcca aaaatctcgc
    14641 cgagcaagcc ggtatgccgc ctatcgaccc aaatgaaatg ggaatgccta acaaattaga
    14701 agtagccaaa agattatata atgtgccgat gtatcagcga tttttagggc aattttacgg
    14761 tgaaaaggta tgggaaagtt atgaaagcgt ttatgcggca atggaagatg cgattgcgac
    14821 attccaacaa gaaaacaaac ttctcgctcc atttgattca aaatatgatc tatttttaaa
    14881 aggcgaggta caattaactg cattggaaga aaaaggcaga gctttatttt tcaataaagc
    14941 tcaaactaat tgtagtagtt gccaccaatt acagagtgct gcaaaccacc aagaagaaac
    15001 ctttagcaac tatcactatt acaatgtagg cgtacccggt aacccagact tgatagcggt
    15061 taacggcttc gcaccagact tcgtcgattt aggcttattt gaaaacccaa atgttaaagg
    15121 tgatgaaaca caaaaaggca aattcaaaac cccaacattg cgtaatgttg ccgtgactgc
    15181 actttatatg cacaacggtg tgtttaaaga attaaagact gttttgctgt ttttagacag
    15241 ctacaataac ccggagcgaa aaatcaaccc tgaaaccggc acggcgtggc aacaagcaga
    15301 atttgaaccc actatcgccc accaaaaact caaagccaaa cccttaagcg atgaagaaat
    15361 tgatgccctc gaagcatttt taaaaacctt gacggatgag cggtatagaa aataaggcta
    15421 caagcggtaa aattttgggg aaattttacc gcttgcgttt aactaccgtg ttagctcact
    15481 ttctaacttt tccaatgcct gcaaatcttc ccaatctgcc tctttctgtt ttaaacattt
    15541 acgctgaata tcaaattttt cgtattgctc tctgacaaag ctttccattt gtttatgcgt
    15601 gattgttcca ccatttccta atacagaaaa gccgttaaac tcaatcaaca tatctacatt
    15661 tcttcgccag aaacctaacg taagatcttg gttgttcttc acacgtaatt cggcactttc
    15721 aagaaagatc attaccaagc gatttaaaga atccagctca ttattatcta aatagttttt
    15781 cgcaatcaca atatcgcctt tacgcacgac ttttcctttc cagctagtta atcccatatt
    15841 catcttagtc gcatcagcac gttgacaaat caactcagcg gcagtgtgtt tggttattgc
    15901 ataaatgagc ttattttgcg tttctgcaaa aaacatttgc gtatttttat ctgttttgtc
    15961 gtaatcgcta gagagtttga aaagctctcg tactttttga tagaaacgca attcacttgc
    16021 ccgaatctca cgaatctgtt caagtaattc atcgaaataa tctaaacgac cctgcggatt
    16081 tttcaaacgc tcgctatcaa ttaaaaaacc tttttcgaga taagtgcgta atgtgctgtt
    16141 tgcccatttt ctaaactgta ctgctcttgg gctacgaact cgaaacccta tcgccagaat
    16201 catttcaagt gaataatgtt ttacttgata attcttgcca tcttgagcag ttatcaagta
    16261 atccttgata actgaattct catctaactc tttgtctttt aagatatttt taatgtgttc
    16321 ggatatattt tgcacagagg tgtcaaaaag ttctgcaagt tgattttgtg tcagccaagc
    16381 ttcattttca accactaaca aggctacatt gctttt
//
LOCUS       DANLXS010000037        16217 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.037, whole genome shotgun sequence.
ACCESSION   DANLXS010000037 DANLXS010000000
VERSION     DANLXS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16217
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.037"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(29..259)
                     /locus_tag="PXD71_000892"
     CDS             complement(29..259)
                     /locus_tag="PXD71_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291038.1"
                     /translation="MALHRCPECRKRISDSLEHCIHCGFSFKEADLAIYRQKLEQRRL
                     HNQEINRKSVKLQLIWLVIFIAVIALASWITN"
     gene            complement(267..1010)
                     /locus_tag="PXD71_000893"
     CDS             complement(267..1010)
                     /locus_tag="PXD71_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291039.1"
                     /translation="MLNKHINKIINSSFSLAFFTLTSFATAYCYGWGQAIFHGYPWWH
                     VEIGNASMARSLAYVFGTSVILFLCYALGYSLLNKVFQLHYFRNLGWLRVIVLVSVFT
                     VPVILTFYLFTGIVPIYISFIYIFVSGVSVFFFRKRWNNTKFDLDFRKMLLGGSFALF
                     NIFIFLYFSLLSLHIGYLRAGLRTTYDYIEIENQKYYILSTNSDNGYIMGTKIRGNQS
                     FLFFNRDTHQYYHVHIEQCTDSRLCKVIV"
     gene            complement(1181..1519)
                     /locus_tag="PXD71_000894"
     CDS             complement(1181..1519)
                     /locus_tag="PXD71_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6291040.1"
                     /translation="MKLITTIIQPSKLDQVQDALSEIGIQGMTITEVKGFGRQYGHTE
                     QELSQMSRIDFRPKIKVEILVSNYQTDEVIDAILNASYSGQVGDGKIYVANIEQVIRT
                     RTGETDDEAV"
     gene            1778..3676
                     /gene="dnaK"
                     /locus_tag="PXD71_000895"
     CDS             1778..3676
                     /gene="dnaK"
                     /locus_tag="PXD71_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL6291041.1"
                     /translation="MGKIIGIDLGTTNSCVAVMDGDKPRVIENAEGARTTPSIIAYTD
                     KETLVGQPAKRQAITNPKNTLFAIKRLIGRRFTDAEVQRDIEIMPFEISKADNGDAWV
                     TVKGDKLAPPQISAEILKKMKKTAEDFLGEPVTEAVITVPAYFNDAQRQATKDAGRIA
                     GLDVKRIINEPTAAALAYGLDSKKENQTIAVYDLGGGTFDISIIEIDNFDGEQTFEVR
                     ATNGDTHLGGEDFDNRVINYLVEEFQKEQGVDLRNDPMAMQRVKEAAEKAKIELSSAQ
                     ETEVNLPYITADATGPKHLNIKVTRAKLESLVEDLVNRSLEPLKTALADAGLSVGDIN
                     DVILVGGQTRMPLVQKKVADFFGKEPRKDVNPDEAVAIGAAVQGGVLSGSVTDVLLLD
                     VTPLSLGIETMGGVMTTLIEKNTTIPTKKSQVFSTAEDNQSAVTIHVLQGERKRAADN
                     KSLGQFNLEGINPAPRGMPQIEVTFDIDANGIINVSAKDKNTGKEQQIKIQASSGLSD
                     AEVEQMVRDAEANAEADKKFEELVQARNQADGIAHATRKQITEAGDALNADDKAKIEA
                     AISELETAAKGEDKAEIEAKIEALIKASEPLMQAAQAKAQGGEQPQQSQKDDGVVDAE
                     FEEVKDNK"
     gene            3818..4120
                     /locus_tag="PXD71_000896"
     CDS             3818..4120
                     /locus_tag="PXD71_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291042.1"
                     /translation="MLKLYPTTENVQPPFYKIYDHKSGETVIATETENSNTAILMDDF
                     VWFEQIPTTEALLADTWSVIENRSKQGLLKRAYTKDFEKHLADGKVEIYIAVNPHC"
     gene            4343..5482
                     /gene="dnaJ"
                     /locus_tag="PXD71_000897"
     CDS             4343..5482
                     /gene="dnaJ"
                     /locus_tag="PXD71_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL6291043.1"
                     /translation="MAKKDYYEVLGLSKGASEKDIKRAYKRLAAKHHPDKNQGSKESE
                     EKFKEITEAYDVLTDSEKKAMYDQYGHAAFEQGGAGAGGFGGFGGGGFGGFEDIFSEM
                     FGGGFGGGGRRQRVVRGDDLRYDIEITLEEAVKGCKKDIRIQTLAECDTCHGTGAEAG
                     SKVETCSHCHGSGRVRRQQGFFVTEAVCPSCHGTGKRIEKPCRSCHGDGRVQKAKNLS
                     VTIPAGVDTGNQLRLSREGAAGENGAPAGDLYVVIHVKEHDIFERDGSNLYCEVPISF
                     TLAALGGEIEVPTLDGKLKLKIPAETQTGKLFRVRGKGVTSPRGGYAGDLICKVVVET
                     PVNLTEEQKALLRQFEESLEGQGKHRPKHEGFFDGVKKFFDNLGK"
     gene            5770..7278
                     /locus_tag="PXD71_000898"
     CDS             5770..7278
                     /locus_tag="PXD71_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDL6291044.1"
                     /translation="MSKCPVTHLTMNNGAPVVDNQNSLTAGPRGPLLAQDLWLNEKLA
                     NFVREVIPERRMHAKGSGAFGTFTVTNDITKYTRAAIFSQVGKKTEMFARFTTVAGER
                     GAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNMRS
                     ATNNWDFWTLLPEAFHQVTIVMSERGIPASYRHMHGYGSHTYSFINAANERFWVKFHF
                     HTQQGIKNLTNEEAAAIIANDRESHQRDLYEAIERGDFPKWKMFVQIMPETDADKVPY
                     HPFDLTKVWPKSDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGISVSPDRMLQ
                     ARLFNYADAQRYRLGVNHHQIPVNAPRCPVHSNARDGQGRVDGNYGSTIHYEPNSFGK
                     WQEQAQYAEPPLKINGDAAHWNYREDDADYFSQPRVLFNMMNDEQKQALFNNTAAGMG
                     DALDFIKYRHIRNCYACDPAYGEGVAKALGMTVEDAIAARATDPALGQPGLL"
     gene            complement(7343..7564)
                     /locus_tag="PXD71_000899"
     CDS             complement(7343..7564)
                     /locus_tag="PXD71_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL6291045.1"
                     /translation="MNVRLPASGLVCPFPLVEAKAAMAKLNKGDSLTIEFDCTQATEA
                     IPNWAEEEGYEVTDYQQLGDALWEITVVK"
     gene            complement(7593..8102)
                     /locus_tag="PXD71_000900"
     CDS             complement(7593..8102)
                     /locus_tag="PXD71_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL6291046.1"
                     /translation="MLFTGLLCGFLLGFVMQRGRFCITGAFRDLYVTKNSRMFVALLI
                     AITVQSIGIWLLYEAGSFSSPAEDLPLLAVIIGAFLFGIGIIYAGGCATGTWYRAGEG
                     LIGSWVALVIYGLFSASMRTGVLAPLNQELKSNVIQHRTIYETFGISPWVLVFILSVI
                     TLALSAHSI"
     gene            complement(8209..9543)
                     /locus_tag="PXD71_000901"
     CDS             complement(8209..9543)
                     /locus_tag="PXD71_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL6291047.1"
                     /translation="MQLYSYICLLFGLAMFISFFTRKLNENIQTTIAITASALVGSLL
                     ILTFGSLGWFNVDKLAISIFEQLDFKSFLLNGMLGFLLFAGSLGIKLPLMKEQRREIA
                     VYALFSTLVSTFLIGALIYGVARLLGLELGFVYCLLFGSLISPTDPIAVLAIIKSLKA
                     PKRLSMHVEGESLFNDGIGLVIFTTIFAVAFGGQEPNLSGITGLFLQEAVGGIVFGLL
                     TGFVAHKFISATDDGSLEILITLTIPTVGFVIANYLHVSGALTMVVAGIMIGNWTRRS
                     GFSEQSQKYLDHFWEMIDHSLNYLLFLLIGLSTLLVDFTFIGGVLMLAAIPICLLARY
                     ISLWIPYQVLKRFRTYNPYTLKIMTWGGLRGGLALAMALSVPAGAAVVSSSEGNLDVR
                     DLILLMTYAVVTFSILVQGSTVEPMIKKSKLVDPRKVALNKLGAPDDVIPHI"
     gene            complement(9654..10802)
                     /gene="dapE"
                     /locus_tag="PXD71_000902"
     CDS             complement(9654..10802)
                     /gene="dapE"
                     /locus_tag="PXD71_000902"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDL6291048.1"
                     /translation="MKNEIISLAQNLIRRDSISPQDKGCQQEIAQRLEKLGFNIEWLP
                     FGDTLNLWATHGSENGKTEPCIVFAGHTDVVPVGDESQWAYPPFSAEIVDGMLYGRGA
                     ADMKGSLSALVVAAETFVQHHPNHKGKVALLITSDEEAAAKDGTVKVVETLMARNEAV
                     HYAVVGEPSSSKVFGDVIKNGRRGSITADLYIEGIQGHVAYPHLAENPVHTSLGFLTE
                     LTTYQWDNGNEFFPPTSLQIANIKAGTGSNNVIPGELYVQFNLRYCTEVTDEIIKAKV
                     AEMLEKHGLKYRISWNLSGKPFLAGNGKLVEAALQAVENVTKITPRLDTGGGTSDGRF
                     IALMGAEVVEFGPLNATIHKVNECVSADDLAHCGEIYYQILEKLLATA"
     gene            complement(10932..11279)
                     /locus_tag="PXD71_000903"
     CDS             complement(10932..11279)
                     /locus_tag="PXD71_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDL6291049.1"
                     /translation="MTTLVYGIKNCDTVKKALKWLEDHNQNPQLHDYRVDGLNADWLV
                     EMEAKFGWENLVNKRSTTWRNLDEEIKNNLNRESALKVLTEQPTLIKRPIVIKEDIAL
                     IGFNEKEYVAAFN"
     gene            complement(11279..12137)
                     /gene="folP"
                     /locus_tag="PXD71_000904"
                     /pseudo
     CDS             complement(11279..12137)
                     /gene="folP"
                     /locus_tag="PXD71_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
     gene            complement(12246..13208)
                     /gene="rluC"
                     /locus_tag="PXD71_000905"
     CDS             complement(12246..13208)
                     /gene="rluC"
                     /locus_tag="PXD71_000905"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDL6291050.1"
                     /translation="MTTEKVISAGVQFFTISEDEAGQRLDNFLLAKLKGVPKSLIYRI
                     VRKGEVRVNKGRNKPEYKLQPDDLVRVPPVRVAEKNEAPISTKLNKVAELESQILYED
                     ESMLVINKPSGIAVHGGSGLSFGVIEALRALRPQARFLELVHRIDRDTSGILLVAKKR
                     SALRSLHEQLREKVVQKDYLALVRGQWQAHTKVIKAPLLKNELASGERIVRVSEEGKP
                     SETRFSIEERYANATLVKASPITGRTHQIRVHTQYAGHPIALDDKYGDAEFDSKMKEV
                     GLQRLFLHAHAIRFEHPKTGEEMVITAPLDKTLKGVLAKLRANY"
     gene            13317..14219
                     /locus_tag="PXD71_000906"
     CDS             13317..14219
                     /locus_tag="PXD71_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL6291051.1"
                     /translation="MTEKVTETAPNIDVVAETEKVIHKVSNMNIETMLNEWIIPYGTK
                     ILLAIAIFVVGKFLARLISRLLGKAALKSTKDEMLQSFIVSISYFLLLLIVVIASLSQ
                     IGINTSSLVALIGAAGLAIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGVVE
                     QMGLLVLELRTGDNKTVLLPNGKVFSDSIINYSNNPTRRIDFTFDISYESNLKEAKEI
                     IQRILDANTYVLKDPAPVVAVGALAPHSVQLVVRPWVQTPNYWAAYWEIIEQVKLAFG
                     EAGISIPYNQMELHIKSGSLENKA"
     gene            14317..14814
                     /gene="rraA"
                     /locus_tag="PXD71_000907"
     CDS             14317..14814
                     /gene="rraA"
                     /locus_tag="PXD71_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDL6291052.1"
                     /translation="MRIDTSALCDLYSDQVDVVEPIFSSFGGASSFFGKITTVKCFES
                     NGLIAEVLEEEGEGRVLLVDGGGAVRRALIDAELAQLAVDNGWEGIIVYGAVRQLDIL
                     ERLDIGIHALAPIPVGADNTDIGEVDTPVNFGGVTFFPEDYVYADLTGIILSQELLDL
                     NDISE"
     gene            complement(14862..16205)
                     /locus_tag="PXD71_000908"
     CDS             complement(14862..16205)
                     /locus_tag="PXD71_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL6291053.1"
                     /translation="MKQLFIFAFTLVLSACSLVVYKPVETIKQVNVERGYRQKNVIAE
                     SAKDGNIVVIMFSGGGTRAAALGYGVLEEFKKVRVKETNKGNTLLENIDIAFGVSGGS
                     ILATYFALEGKDIIPKFADRFLKANFQKDIITEVFSLSNLPRLSSPQYGRGDLLQERL
                     NFALYHGKKFGDLVKNRKGPFAVISATDMNIGQKLTFTQETFDGLCLNLNDLEISRAV
                     AASSSVPLIFAPLTLNNNGGNCHFSMPQEFSAREKAIDGLITKNIEETNHILSLYQDS
                     KERPFIHLVDGGLTDNLGLASVLEISDVMGIEEMYQYIHQLRVKNIVVINVNAQNEVS
                     SEIDKTANVPGVADVVNTIVNVPIDRTTQINLQRYRQFTDGWNEYANKQPSKIEMHFV
                     SLGLKELPDGDLKKDVLNIATSFYLPEEDVDKLREAARVLLQQSQEYNAALKTLQ"
BASE COUNT         4940 a         3475 c         3264 g         4538 t
ORIGIN      
        1 aaaacccggt aaataccggg cttttatttc aattggttat ccaacttgcg agagcaatta
       61 ccgcaataaa aataactaac caaataagtt gtaattttac gcttttgcgg ttgatctcct
      121 ggttgtgtaa tcggcgctgt tctaactttt gccgatatat cgctaaatcg gcttctttaa
      181 acgagaagcc acaatgtatg caatgttcta aactgtcgct aatacgcttt cgacactccg
      241 gacaacggtg taatgccata attacctcaa actattactt tgcataatcg gctatccgta
      301 cattgttcaa tatgaacatg gtagtattga tgagtatcac gattgaaaaa caagaaagat
      361 tgattacctc ttatttttgt tcccataatg tagccgttat cactgttggt ggataatatg
      421 tagtattttt gattctctat ttcgatgtaa tcataggtag ttcttagtcc ggcacgcaaa
      481 taaccaatat gcaatgagag caaactgaaa tagagaaaaa taaagatgtt aaacagggca
      541 aaacttcctc ctaatagcat ctttctaaag tccagatcaa acttggtatt attccatctc
      601 ttgcggaaga agaatacgct cacaccactt acaaaaatat agataaaaga gatataaatg
      661 ggcactatcc cggtaaataa gtaaaaagta agaataacag gcactgtaaa cacagaaacc
      721 aacacaatca cccttaacca acctagattt cggaaataat gcagctgaaa aactttattt
      781 agtaaactat aacctagtgc ataacacaag aataagatga cggaagtacc gaatacataa
      841 gccaaacttc ttgccatact ggcattgccg atttcaacat gccaccaagg gtagccatga
      901 aatatggctt gtccccaacc gtaacagtaa gcggtagcaa aactagttaa tgtaaaaaat
      961 gcaagagaaa atgaagaatt gataatttta ttaatgtgtt tatttagcat aacaaagaat
     1021 gtctatttcc attaaaacct aactattttt gattactata ataacccgta ttacacaaaa
     1081 aacaaacatt taattcacac caaattagaa aatttgcttt agtaaggttg aaaatataac
     1141 tctaaaacac gataataaaa agcttctctg ctagcaatgg ctagactgct tcatcgtcag
     1201 tttcgccggt acgggtgcgg ataacttgtt caatattcgc tacataaatt ttgccatcac
     1261 ccacttgccc agaataggat gcattgagaa tggcatcgat cacttcatcg gtttggtaat
     1321 tagaaaccaa tatctccacc ttaattttag gacgaaagtc aatgcggctc atttggctaa
     1381 gctcttgttc ggtatgccca tattggcgtc caaagccttt tacttcggta atagtcattc
     1441 cttgaatacc gatttctgaa agtgcatctt gcacttggtc taattttgaa ggttggataa
     1501 tggtagtaat cagtttcata ttatatcctt ctctgtttga gttgccgtaa gtataaaaag
     1561 ctattgaggg gtatgcaagg taggaggtat tttctccgtg aaatattctg ccaaatctcc
     1621 cccacataca agcggttact ttttccaaaa attttgcaat ttttttattt ttgcccttga
     1681 acagtgcatt ttcgtcccta tttatgattt cgtctgaacg ggaaatgcaa tcccgtttct
     1741 tattcaaact gaatttatta ttataggaat cataaaaatg ggtaaaatca ttggtattga
     1801 cttaggtaca acaaattctt gtgttgcggt aatggacggc gacaaacctc gtgtaattga
     1861 aaatgcggaa ggggcaagaa caacgccatc tatcatcgct tacaccgata aagaaacctt
     1921 agttggtcag ccggcaaaac gtcaggcaat tactaacccg aaaaatactt tatttgcaat
     1981 caagcgttta atcggccgtc gttttactga tgctgaagtg caacgtgata ttgaaattat
     2041 gccgtttgaa atcagcaaag cagataacgg cgatgcgtgg gtaaccgtaa aaggcgataa
     2101 attagcaccg ccgcaaatct ctgctgaaat cttgaaaaaa atgaagaaaa ccgcagaaga
     2161 tttcttgggc gaaccggtaa ccgaagcggt tattactgtg cctgcatact ttaacgatgc
     2221 acaacgtcag gcaactaaag atgccggtcg tattgcaggt ttagatgtaa aacgtatcat
     2281 caacgaacca acagcggcag cgcttgctta tggtttagat tctaagaaag agaaccaaac
     2341 gattgctgtt tacgacttag gtggtggtac attcgatatt tcgattatcg aaattgataa
     2401 ctttgacggc gagcaaactt ttgaagtgcg tgcaaccaac ggcgatactc acctaggtgg
     2461 tgaagacttc gataaccgtg taatcaacta cttagtggaa gaattccaaa aagaacaagg
     2521 cgttgattta cgcaacgacc caatggcaat gcaacgtgtg aaagaagcag cggaaaaagc
     2581 gaaaatcgaa ctttcttctg cacaagaaac tgaagtgaac cttccataca tcacggcaga
     2641 tgcaaccggc ccgaaacact taaacattaa agtaacccgt gcgaaattgg aatctttagt
     2701 tgaagattta gtaaaccgtt cgttagagcc gttaaaaacc gctttagctg atgccggttt
     2761 atcagtaggc gacatcaacg atgtgatttt agtcggtggt caaacccgta tgccgttagt
     2821 acagaaaaaa gtggcagact tctttggcaa agaaccgcgt aaagacgtta acccggacga
     2881 agctgtggca atcggtgcgg cggttcaagg cggtgtgtta agtggttcgg taacagacgt
     2941 attgttatta gacgttactc cgctttcact tggtatcgaa acaatgggtg gtgtaatgac
     3001 taccttaatt gagaaaaaca ctacgattcc aaccaagaaa tcacaagtgt tctcaacagc
     3061 ggaagataac caatctgcgg taactatcca cgtgttacaa ggtgagcgta aacgtgcagc
     3121 ggataataaa tctttaggtc aattcaacct tgaaggtatt aaccctgctc cacgcggtat
     3181 gccacaaatt gaagtaacct ttgatattga tgctaacggt attatcaatg tctctgcaaa
     3241 agataaaaac actggcaaag agcaacaaat caaaattcaa gcatcttcag gcttaagcga
     3301 tgctgaagtt gaacaaatgg tgcgtgatgc agaagcgaat gcggaagcag acaaaaaatt
     3361 tgaagagtta gttcaagctc gtaaccaagc agacggtatt gctcacgcaa cccgtaagca
     3421 aattaccgaa gcaggcgatg cgttaaacgc agacgataaa gcgaagatcg aagcggctat
     3481 ttctgagtta gaaaccgcag ctaaaggcga agataaagcc gagatcgaag cgaaaatcga
     3541 agcgttaatc aaagcttcag aaccgttaat gcaagcagca caagcaaaag cacaaggcgg
     3601 cgagcaacca caacaatctc aaaaagatga cggcgtggtc gatgcggaat ttgaagaagt
     3661 gaaagataat aaataatctt gcgtaacttg taggggcgaa atatctttcg ccccttttgt
     3721 gtagatatat accacctagc acgagccgtg ctaggttaca cataattcag cccctttggg
     3781 gctgtgcaag cggtgctttt caaaggaaat tttacaaatg ctaaaactct accctacaac
     3841 ggaaaatgta cagccaccgt tttataaaat atacgatcat aaaagtggcg aaacggtcat
     3901 tgccacagaa acggaaaaca gcaataccgc gattttaatg gacgattttg tttggtttga
     3961 gcaaattcca actaccgaag ctcttttggc agacacttgg tctgtgattg aaaaccgttc
     4021 caaacaaggt ttgctcaaac gagcttacac caaagatttt gaaaaacatt tagcagacgg
     4081 caaagtagaa atttatattg cggtgaatcc gcattgttag gcttttgata gcgcaagcgt
     4141 ccacgcttgt gtcatcttga gccatcttgg ctagatccca tttaattata taaaactctc
     4201 tccccttgtg ggagagagac cgaaaaatat ttcgttcaga aatatttttc agagagaggg
     4261 ggaattaacc atcctacccc tctcccacaa ggggcgaggg caagcggtca aaaacacaga
     4321 aaaatttaca aacggaacaa caatggcaaa gaaagattat tacgaagtcc ttggactgtc
     4381 taaaggggca agcgagaaag acatcaaacg agcatataaa cgtttggcgg caaagcatca
     4441 cccggataaa aaccaaggca gtaaagagtc ggaagagaaa tttaaagaga ttaccgaagc
     4501 ctatgacgtg ctgaccgaca gcgagaaaaa agcgatgtac gaccaatacg gtcacgccgc
     4561 ctttgaacaa ggcggtgccg gtgcaggcgg attcggtggc tttggcggcg gtggtttcgg
     4621 tggttttgaa gacatcttca gcgaaatgtt tggcggtggc tttggtggcg gtggacgtcg
     4681 ccaacgtgtg gtacgtggcg atgatttacg ttacgacatc gagatcaccc ttgaagaagc
     4741 tgtgaaaggc tgtaaaaaag atattcgcat tcaaacctta gctgaatgtg atacttgtca
     4801 tggtacaggg gcggaagcag ggagcaaagt ggaaacctgt tcgcattgtc acggctccgg
     4861 cagagttcgc cgtcagcaag gtttctttgt gactgaggca gtttgtccaa gttgtcacgg
     4921 tacaggtaaa cgcattgaaa aaccgtgccg ttcttgccac ggcgatggac gtgtacagaa
     4981 agcgaaaaac ctctctgtta ccattccggc aggtgtggat accggtaatc aacttcgctt
     5041 atcgagagag ggagctgcag gcgaaaatgg cgcaccggca ggcgatttat atgtggtgat
     5101 tcacgttaaa gaacacgata tttttgagcg tgacggttcg aatttatact gtgaagtgcc
     5161 gattagcttt acccttgcag ctttaggcgg tgaaattgaa gtgccaactt tagacggcaa
     5221 attaaaactc aaaattccgg cagaaactca aaccggtaaa ctattccgtg tacgaggcaa
     5281 aggggtaaca agccctcgtg gtggctacgc aggggattta atctgcaaag tggtggtcga
     5341 aacgcctgtg aatttaaccg aagaacagaa agcactatta cgacaatttg aagagagttt
     5401 agaaggacaa ggtaaacatc gtccgaaaca cgaaggtttc tttgatggtg tgaagaaatt
     5461 ctttgataac ttaggcaaat aaaatcataa aaagtacata tttcttaaat atgtactttt
     5521 ttattatcta ttctttgttg aaatctactt ctcggccgtt aatttttaca cctacatact
     5581 gcaaaaaata gtggaaaaat gaccgcttat catctttctc agaatacttg acaattaaaa
     5641 tcaactattt aaaattaata tttaatagtc attaattatc aaattgatag attgagaaaa
     5701 aatctacaac acctataatt ctcttcggat taattaccta tcttatttct aatttatgag
     5761 gattctatta tgtctaaatg tcctgttaca catttgacaa tgaacaacgg tgcacctgtt
     5821 gttgataacc aaaacagctt aacagcgggt ccacgtggtc cattattagc gcaagattta
     5881 tggttaaatg aaaaattagc gaacttcgtg cgtgaagtca ttcctgagcg tcgtatgcac
     5941 gctaaaggtt ccggtgcgtt cggtactttc actgtaacta acgacatcac taaatacact
     6001 cgtgcggcaa tttttagcca agtcggtaaa aaaactgaaa tgtttgctcg tttcaccacc
     6061 gttgcaggtg aacgtggtgc ggcagatgct gagcgtgata ttcgtggttt cgcattaaaa
     6121 ttctataccg aagaaggtaa ctgggatatg gtgggtaaca acacccctgt gttcttctta
     6181 cgtgacccac gtaaattccc ggatttaaac aaagcggtta aacgtgaccc acgcacgaat
     6241 atgcgttctg caactaacaa ctgggatttc tggacattat taccagaagc atttcaccaa
     6301 gtgactattg taatgagtga acgtggtatt ccggcttcat atcgtcatat gcacggttat
     6361 ggctcacata cttatagctt catcaatgca gctaatgagc gtttctgggt gaaattccat
     6421 ttccatacac aacaaggcat taaaaactta actaacgaag aagcagccgc tattatcgct
     6481 aacgatcgtg aaagtcacca acgtgattta tacgaagcga ttgagcgtgg cgatttcccg
     6541 aaatggaaaa tgtttgttca aattatgcct gaaactgatg cagacaaagt accataccac
     6601 ccgttcgatt taactaaggt atggccgaaa tcagactatc cgttaattga agtgggtgag
     6661 tttgaattaa atcgtaaccc tgaaaacttc tttgctgatg tggaacaatc tgcattcgca
     6721 ccaagtaact tagtgccggg catcagcgtg tctccagacc gtatgttaca agctcgttta
     6781 ttcaactatg cggatgctca acgctaccgt ttaggtgtga accaccacca aatccctgta
     6841 aacgctccac gttgcccggt tcatagcaac gctcgtgacg gtcaaggtcg tgtggacggt
     6901 aactacggta gtaccattca ctacgaacca aacagcttcg gcaaatggca agaacaagct
     6961 caatatgcag agccaccact gaaaattaat ggtgatgcag cccactggaa ctatcgtgaa
     7021 gacgatgctg actacttcag ccaaccacgt gtattgttca atatgatgaa tgatgaacaa
     7081 aaacaagcat tattcaataa tactgctgca ggtatgggcg atgcattaga cttcattaaa
     7141 taccgtcata ttcgtaactg ctatgcctgt gatccggcat atggtgaagg tgtggcaaaa
     7201 gcattaggta tgacggttga agatgctatt gctgcaagag caacagaccc agcattaggt
     7261 caaccgggtt tactctaatc cttagtaaat acaaaactca aagcaaaagc tctacaaaaa
     7321 agtagagctt ttttattgct aattacttaa ccaccgtaat ttcccacaag gcatcaccaa
     7381 gttgttggta atcggtaact tcatagcctt cctcttccgc ccaatttgga atggcttcgg
     7441 ttgcttgagt acagtcaaat tcaatcgtta agctatcgcc tttattcaat tttgccatcg
     7501 ccgctttggc ttcgactaat ggaaacgggc aaacaagccc tgaggcaggt aaacgtacgt
     7561 tcatctttat tactccttaa aaaattaacc ttttaaatgc tgtgggcgga taatgccaat
     7621 gtaattacac tcaggataaa cactaacacc caaggggaaa taccaaatgt ttcataaatc
     7681 gtgcggtgtt gaattacatt gctttttagc tcttgattaa gtggggctaa cacaccggtt
     7741 cgcatagaag cagaaaacag cccataaata accagtgcaa cccaactgcc gattaaaccc
     7801 tcaccggcac gataccacgt tcctgtagca caaccgcctg cataaataat gccgatacca
     7861 aacagaaatg ccccaatgat cactgcgagc aaaggcaaat cttccgccgg agaactaaaa
     7921 gagccggctt catacaaaag ccaaatcccg atcgattgca ccgtaattgc aattaaaagt
     7981 gccacaaaca tacggctatt ctttgttaca tagagatcac gaaatgctcc ggtaatacaa
     8041 aaacgccctc tttgcatcac aaacccgagt agaaagccac ataataatcc tgtgaataac
     8101 ataaaaaata cctctcaaca aattaacgtg agaggtattt aaatataaag ccgtctagat
     8161 ggctaataat ctttagcaaa atgatatgtt attcagttat aaatgctatt aaatatgtgg
     8221 aattacatca tcaggtgcac ctaatttatt gagtgccact tttcttggat ctaccaactt
     8281 ggattttttt atcatcggtt caaccgttga accttgtact aaaatcgaga aagtgactac
     8341 cgcataagtc attaacaaaa tcaaatcacg tacatcaaga ttgccttctg agctcgatac
     8401 caccgcagcc cctgccggta cggaaagtgc catcgcaagg gctaatccac cacgtaaacc
     8461 gccccaagtc ataattttta aggtgtacgg gttataagta cggaaacgtt ttaacacttg
     8521 gtatggaatc cacaggctga tatagcgtgc aagcaaacaa attggaattg ctgccaacat
     8581 taacacccca ccaataaagg tgaaatcaac taacagtgta cttaagccga taagcaggaa
     8641 taacaggtag tttaatgaat ggtcgatcat ctcccagaag tgatcaagat atttttggct
     8701 ctgttcggag aaaccggaac ggcgagtcca gttgccaatc ataataccgg caactaccat
     8761 tgttaatgcc cctgaaacgt gtaggtagtt tgcaatcaca aagccgactg ttggaatcgt
     8821 tagcgtaatt aaaatttcaa ggcttccgtc atcggttgcg gaaataaatt tatgtgccac
     8881 aaaaccggtt aataagccaa aaacgatacc gcctaccgct tcttgcaaga acagtccggt
     8941 aataccgcta aggtttggtt cttgtccgcc aaaagcaacg gcaaagatag tggtaaaaat
     9001 caccaagccg ataccgtcat taaacagaga ttcgccctcc acgtgcatcg aaaggcgttt
     9061 tggggctttc aggcttttga taattgccaa tacggcaatc gggtcggttg gcgaaatcag
     9121 tgagccaaac agcaaacaat acacaaagcc gagctctaac ccaagtagcc ttgccacgcc
     9181 ataaattaac gcaccgatca agaaagtaga aactaaggtt gaaaataagg cataaactgc
     9241 aatttcccgc cgttgctctt tcattaatgg tagtttaatc ccaagtgaac cggcaaaaag
     9301 caagaacccc agcattccgt ttaacaggaa gcttttaaaa tcgagttgtt caaaaatgga
     9361 aatggcaagt ttatcaacat taaaccagcc gagagaaccg aaggtaagaa taagaaggga
     9421 gccgacaagg gcagaggctg taattgcaat agtggtttga atattttcgt ttaattttcg
     9481 ggtaaaaaac gaaataaaca tcgcaagccc aaacagcaag cagatataag agtagagttg
     9541 cataacattt acctataaat ataaaaactg ccgctagtgt aaacgctgtt taacagaata
     9601 gctacataaa aaataggcag aatacaattt gataacattc tgcctattta gggttatgca
     9661 gttgccaata atttttctaa aatttgataa taaatttcgc cacaatgtgc caaatcatcc
     9721 gcactcacac actcattcac tttgtgaata gtggcgttca gtggtccaaa ttccactacc
     9781 tctgccccca ttaatgcaat aaaacgaccg tccgaagtac cgccacctgt gtctaaacga
     9841 ggcgtaattt ttgtgacatt ttcgaccgct tgtaaggcgg cttcgaccaa ttttccattg
     9901 ccggcaagga atggtttgcc tgataaattc caacttattc ggtatttcaa accgtgtttt
     9961 tcgagcattt ccgctacttt ggctttgatg atttcatcgg tcacttcagt gcaataacgt
    10021 aagttaaact gcacataaag ctcgcccgga atcacattat tactgcctgt gccggctttg
    10081 atattggcaa tttgcaaact ggtaggcggg aaaaattcgt taccgttatc ccactgataa
    10141 gtggtcagct cggttaaaaa acccagtgac gtatgcaccg gattttccgc caaatgtgga
    10201 taggcaacgt gcccttgaat gccctcgata tagagatctg ccgtaattga accacgtctg
    10261 ccgtttttaa tcacatcgcc aaacaccttg ctactagacg gttcgcccac taccgcataa
    10321 tgtaccgctt cattgcgagc cattaaggtt tcaaccactt ttaccgtacc gtctttggct
    10381 gctgcctctt catcggaggt aatcaataat gccactttgc ccttatggtt tgggtgatgt
    10441 tgcacgaaag tttctgccgc cacaactaac gccgagagcg agcctttcat atctgccgcc
    10501 cctcgcccat aaagcattcc atctacaatc tcagccgaaa acggcggata agcccattgg
    10561 ctttcatcgc ctaccggcac cacatctgta tgcccggcaa acacaatgca aggctctgtt
    10621 ttaccatttt ccgagccgtg cgttgcccat aaattcaagg tgtcgccaaa cggcaaccat
    10681 tcaatattaa agcccagttt ttcaagacgt tgtgcaattt cttgctgaca gcctttgtct
    10741 tgtgggctga tggaatcacg gcggattagg ttctgtgcaa gggaaataat ttcatttttc
    10801 ataggttaca agcggttgtt ttgggtgaat tttttgctat atatcctgta ggggcgggtc
    10861 ctgtgcccgc ccgaacaatc ctaaagacta cggacaggca caggacctgt ccctacaagc
    10921 aggggaaaaa attaattaaa cgcagccaca tactctttct cattaaaacc aatcaaagca
    10981 atatcctctt taatcacaat cgggcgttta attagggtcg gctgttcggt tagcactttt
    11041 aatgccgact cacggttaag gttgttttta atttcctcat caaggtttcg ccaagtggtg
    11101 ctacgtttat taactaagtt ttcccaaccg aattttgctt ccatttccac taaccaatcg
    11161 gcatttaaac cgtccacacg gtaatcgtgc agttgtgggt tttgattatg atcttcgagc
    11221 catttcaagg ctttttttac tgtgtcgcaa tttttaatgc cataaactaa ggttgtcatc
    11281 atttttcctc attatctgat tccagcattg cctgccagat ttttaacgca tcggcggttt
    11341 ctgccacatc gtgcacccgt aaaattttcg ccccattttg agcggctaat aacgccccta
    11401 ctacagagcc aagtagccgt tgttctaccg gtttatctaa caccgcacca atcattgatt
    11461 tacgggaaag tccggcaagc acagggaagc ctgaatccac aaaggcttta aggtgtttca
    11521 ataattggta attgtgtttc acgcttttac caaagccaaa accggggtct aaaataatgt
    11581 gttctttggg aatgcctgcg gtgatgcact cgaaaatccg ttgatttaag aaatctgcca
    11641 catcttccaa cacatcatca tagtgcggat tttgttgcat tgtttgcggg ttgccttgca
    11701 tatgcattaa acaaaccggc aagcccaatt ctactgccgc tgccaacgca ttcggctcgg
    11761 ttaaggctcg aatatcgtta atgatatcca taccgacttt tgccgactct ttgaatactt
    11821 ctgctttgga gctatcaacc gaaatcaagc aatcaaaacg gctgcggatc gcctccacca
    11881 ccggcacaac acgctctaat tcttgttcta agctcacaat cgccgcatta gggcgggtgg
    11941 attcaccgcc aatatcaata atgcttgccc ccatcagcta acattttttc cgcttggaac
    12001 aaggctttat cgagggaaaa aaacttgccc gaatcggaaa atgaatccgg cgtgaagttt
    12061 aagatcccca taatttgcgg tgtggttaaa tctaacaagc ggtcattttt ttgaaaattt
    12121 ttgaaatgga tttgcatatt tttgcttata ttttgttggt gcaagcgtcc acgcttgtac
    12181 cactctatgt tgatttacag cacaagcgtg gacgcttgcg ctatcttact ctctcattaa
    12241 gttatttagt aattagccct taattttgcc aatacgcctt ttaaagtttt atccagcggg
    12301 gcggtaatga ccatttcttc gccggttttg gggtgttcaa aacgaatagc atgggcgtgc
    12361 agaaataaac gctgtaagcc cacttctttc attttgctgt caaattccgc atcaccatat
    12421 ttgtcatcta acgcaatcgg gtgtccggca tattgggtat gcacacggat ttggtgggtt
    12481 cttccggtaa taggcgaagc tttcaccaaa gtggcattgg cataacgctc ttcaatgctg
    12541 aaacgggttt cggacggttt accctcttca ctcacccgta caattcgctc accgctggca
    12601 agttcgtttt ttagcaatgg agctttaatc actttggtat gagcctgcca ttgccctcgc
    12661 acaagggcaa gatagtcttt ttgtactact ttttcacgca gttgttcgtg caaggaacgt
    12721 aacgccgaac gctttttcgc caccagcaaa atgcctgatg tatcacggtc tatacggtgt
    12781 accaattcca agaaacgagc ctgtggacgt aatgcacgca aggcttcgat cacaccaaaa
    12841 cttaaaccac tgccaccgtg tactgcaatg cctgaaggtt tgttaattac tagcattgac
    12901 tcatcttcat acagaatttg gctctccagc tccgccactt tgtttaattt agtcgaaatt
    12961 ggggcttcat ttttttccgc cacacgcacc ggcggcacac gcactaaatc atcaggctgt
    13021 aatttatatt cgggtttgtt tcgtccttta ttcacccgca cctcgccttt tcgcacaatg
    13081 cggtaaatca aacttttcgg cacgcctttg agctttgcca ataaaaaatt atctaaacgc
    13141 tgtccggctt catcttcact gatcgtaaaa aattgaacgc cggcactaat tactttttct
    13201 gttgtcatta tcttgtcttt tagagaaaat tttgctctaa attctagcac aaaaccgctt
    13261 agcgagtgag gcaaattcgc taggcttatt tttcatttta attttaagga aacaatatga
    13321 ctgaaaaagt caccgaaaca gctccaaaca ttgacgtggt tgccgaaacc gaaaaagtaa
    13381 tccacaaagt cagtaatatg aatattgaaa ctatgctaaa tgagtggatt atcccttatg
    13441 gcaccaaaat tttactggcg attgcgattt ttgtagtcgg gaaatttctt gcccgcctaa
    13501 ttagccgttt actcggtaaa gccgcattaa aatcaaccaa agatgaaatg ctgcaaagtt
    13561 ttattgtctc aatcagctat tttttactgc tgttaattgt ggtgattgcc tcgctttcac
    13621 aaatcggcat taacaccagc tcgctggtcg cattaatcgg tgctgccggt ttagccatcg
    13681 gtttatcatt acaaaactca ttacaaaact ttgctgccgg cgtgatgttg ttaatcttca
    13741 agccatttcg caaaggggat ttaatcgaaa ccggcggaat gaccggcgtg gttgaacaaa
    13801 tggggctttt agtgttggaa cttcgcaccg gcgataacaa aaccgtgtta ttaccaaacg
    13861 gcaaagtgtt ttctgacagc atcatcaact actctaacaa cccgacacgc cgtattgatt
    13921 tcacctttga tatttcttat gaatctaact taaaagaggc gaaagagatt atccaacgca
    13981 ttttagacgc caatacttat gtattaaaag atccggcacc tgtagtagca gtcggcgctt
    14041 tagcaccgca tagtgttcaa cttgttgttc gcccttgggt tcagacacca aattactggg
    14101 cagcatattg ggaaattatc gaacaagtaa aattagcctt tggtgaagca ggtatatcta
    14161 ttccttacaa ccaaatggaa ttgcatatta aaagcggttc actagaaaat aaagcataag
    14221 aaaaaagcct gtaagcggtc ttttttcgat aaaattttgc aaattttggg cgaaggttta
    14281 tactttagct aaataatcta cgaacaggag catattatgc gtattgatac ctctgcactt
    14341 tgcgatcttt attctgacca agttgatgtg gttgaaccaa ttttctcaag ctttggcggt
    14401 gcttcttcct tctttgggaa gattaccacg gttaaatgct ttgaaagcaa cggtttaatt
    14461 gctgaagttt tggaagaaga gggtgaaggg cgagtgctgt tagtcgatgg tggcggtgcg
    14521 gttcgtcgtg ccttaattga cgcagagctg gctcaacttg ctgtagataa cggctgggaa
    14581 ggtatcatcg tttatggtgc ggttcgccaa ttagatattt tggaaagatt agatatcggt
    14641 attcatgctt tagcgccaat tccagtggga gcagataata ccgacattgg cgaagtggat
    14701 acgcctgtga attttggcgg tgtaaccttc ttccccgaag attacgttta tgctgactta
    14761 accggcatta ttctttcgca agagttgttg gatttaaacg acatcagcga ataaatattt
    14821 gcaaaaaaat accaaaaacc gaccgcttgg tcggtttttt actactgcag tgtttttaat
    14881 gccgcattat attcttgaga ttgctgcaat aaaacccgag cagcttcacg taatttatct
    14941 acatcctctt ccggcaaata aaatgatgtt gcaatattta gcacatcttt ttttaaatca
    15001 ccatcaggta attcttttaa acctaaactg acaaaatgca tttcgatctt tgatggctgt
    15061 ttatttgcat attcattcca accatctgta aactggcgat aacgttgtaa attaatttgt
    15121 gtcgttctat caattggcac attaactatt gtattcacta catcagccac ccccggtaca
    15181 tttgcggtct tatcgatctc gctagatact tcattttgcg catttacatt aatcactaca
    15241 atatttttca ccctaagctg atgaatatac tggtacatct cttcaatacc cataacatca
    15301 gatatttcca gcacactggc aagccctaaa ttatctgtta atccaccatc aactaaatgg
    15361 ataaaggggc gttctttact atcttgataa aggctgagta tatgattcgt ttcttcaata
    15421 ttttttgtaa ttagtccatc aattgctttt tctctagcac taaattcttg tggcatacta
    15481 aaatggcaat ttccaccatt gttattcagt gtaagcggcg caaaaatcag aggaacagaa
    15541 ctagaagcag caacagcacg ggaaatttct aaatcattca aatttaaaca aagcccatca
    15601 aaggtttctt gcgtaaaggt cagtttttga ccaatgttca tgtctgttgc gctaatcaca
    15661 gcaaaaggtc ctttacgatt tttcactaaa tcaccaaact ttttaccatg ataaagggca
    15721 aaatttaagc gttcttgcaa taaatcacca cgcccatatt gaggagaact taaacgagga
    15781 agattggata acgaaaacac ctctgtaata atatcttttt gaaagttcgc ttttaaaaaa
    15841 cggtcagcaa atttaggaat gatatccttt ccttccaacg caaaataagt ggctaatatc
    15901 gatccacctg atacgccaaa ggcaatatct atattctcta ataaagtatt acctttattt
    15961 gtttctttca cccttacctt tttaaactct tccaacactc cataaccaag cgccgccgct
    16021 cttgttccac cacctgaaaa catgatgaca acaatattac cgtcttttgc actttcagcg
    16081 atcacatttt tttgtcgata acctctttca acatttacct gtttaattgt ttcaactggc
    16141 ttataaacaa ccaaactgca agcagataac actagcgtaa aggcgaagat gaaaagctgt
    16201 tttattttgt ttagaaa
//
LOCUS       DANLXS010000038        16093 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.038, whole genome shotgun sequence.
ACCESSION   DANLXS010000038 DANLXS010000000
VERSION     DANLXS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16093
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.038"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            12..1394
                     /gene="nhaC"
                     /locus_tag="PXD71_000909"
     CDS             12..1394
                     /gene="nhaC"
                     /locus_tag="PXD71_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL6291054.1"
                     /translation="MNNYLLKLKPMTAVILSLLTVFILGFTMIGLGWVPQISILAVIV
                     MLLLFGLINKIPFKNMQEGMASGLMSSIGAVYLFFFIGLLVSALMMSGAIPTLMYYGL
                     SVISPELFYISAFALSSVVGIALGSSLTTTATIGVAFIGMASAFGANPAIVAGAVVSG
                     AFFGDKTSPLSDTTTIAASVVGIDMFAHIRNMLYTTIPAWILTALFLWFLTEPTDAQN
                     LANIENLKTNLQETGLIHGYSLLPFVTLILMAIKRVNSIYIIVTTVTLSLIITYIHSS
                     PTLEQLGNYFFTSYKPVEGVDLGEVASLVSRGGINSMFFTITIVLLALSLGGLLQILG
                     ILPALLNGISGFLTSTGRVTLVVASTAVGINVLIGEQYLSLLLSGNTFRSTYERLGLE
                     VKNLSRTIEDAGTVINPLVPWGVCGVFISSVLNVPVLDYLPYAFFCYICLILTLIFGF
                     TGVTISKKAN"
     gene            complement(1437..2633)
                     /gene="ispC"
                     /locus_tag="PXD71_000910"
     CDS             complement(1437..2633)
                     /gene="ispC"
                     /locus_tag="PXD71_000910"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDL6291055.1"
                     /translation="MKKLVILGSTGSIGKSTLSVVEHNPNPNQYEVFALVGGKNVELM
                     LEQCRQYQPTFAAMDDEKSAQALKTALATLGSKTEVLAGQAAICELAAHSEVDMVMAA
                     IVGAAGLLPTLSAVKAGKTVLLANKESLVTCGQIFIDEARKSGAKLLPVDSEHNAIFQ
                     SLPPEAQQKVGFCPLAELGVSKIILTGSGGPFRTKPLNEFDAITPAQAVAHPNWSMGK
                     KISVDSATMMNKGLEYIEARWLFNATAEEMDIIIHPQSIIHSMVRYIDGSVIAQMGNP
                     DMCTPIAHTMAYPNRINAGVPPLDFFKLKELTFIEPDFARYPNLKLAIEAFAEGQYAT
                     TAMNAANEIAVEAFLNEQIRFTDIVNVNRTVVENIAPIQVSEIADVLHIDKLARELAK
                     QAVLQL"
     gene            complement(2752..4950)
                     /gene="relA"
                     /locus_tag="PXD71_000911"
     CDS             complement(2752..4950)
                     /gene="relA"
                     /locus_tag="PXD71_000911"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDL6291056.1"
                     /translation="MVAIRYSHQLEPNNFELSSWCASLNMSPVTFEQIHNAWSYAQEK
                     LDTDYSLMWYGIEMVEVLHGLNMDDDSLVAAMLFPLVKHNVIDLVQLKEDFSNDIKNL
                     VKGVLEMENIRQLSANNVSDLQIDNIRRMLLAMVDDFRCVVIKLAERIAYLRDSNHHS
                     EEDTVLAAKECSHVYAPLANRLGIGQLKWELEDYCFRILHPVSYTQIAKFELGERRVD
                     RERYIANFVADLTACLSPEIKNVQIYGRPKHIYSIWKKMQKKRLRFNQLFDIRAVRVI
                     VPTLEDCYTALGIIHTKYTHLPEHFDDYIANPKPNGYQSIHTVVLGEGEKTIEVQIRT
                     QQMHDDAELGVAAHWKYKEGATAGRSGYEEKIVWLRKLLEWQNDLADSGDMVAEMRSQ
                     IFDDRVYVFTPRGEVIDLPQKATPLDFAYAIHSEIGHRCIGAKVAGRIVPFTYHLQMG
                     DQVEIITQKEPNPSRDWLNPNTGFVNTGKARSKIIAWFKKLDREKNIPIGKEALEAEM
                     EKFGFTLKQIEEYALPRYNLKQFDDLYAGIGGGDIRLHQLMHFLQSKLIKPTAEQEDE
                     AVLKQVNKNTNQPKPSKGYVIVEGVGNLMHSIARCCQPIPGDEIVGYITQGRGISIHR
                     ADCEQLFELQSLNPERVVEADWGEGYTSGLSLTIRVIANDRNGLLRDVSAIMANEKVN
                     VLGVSSRTDVKRSLATIDMEIQLNNVEILNKLLARIAQLDDVIEAKRLSS"
     gene            5204..6511
                     /locus_tag="PXD71_000912"
     CDS             5204..6511
                     /locus_tag="PXD71_000912"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDL6291057.1"
                     /translation="MTILALGINHKTASVGLREKVAFVDDKRKLALEQIQTSGLAESV
                     VILSTCNRTELYFHQPNISPREESEENIQWREQCFRWFAQIHQLDESELRECLYFKQN
                     LEAANHLMKVASGLDSLILGEPQILGQVKQAYQYSEDFYQHQQKAMSTKLSRLFQRTF
                     ATAKRVRSETEIGSQAVSVAYAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQH
                     GAKNIMLANRTHIRAEMLAEKLNAPMQILSLSALQLGLNQADVVISSTGSPDTLISKE
                     MVEIAQKQRQFDPLLLIDIAVPRDIDEKAGDLEGVYAYSVDDLQNIIQRNMAQREEAA
                     KQAAEIVKEESKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLEKALQAISQGQDSQKV
                     LSELSYKLTNQLLHAPTQALQAMAKSGNSTGLQSFSRALRLEE"
     gene            6696..8231
                     /gene="der"
                     /locus_tag="PXD71_000913"
     CDS             6696..8231
                     /gene="der"
                     /locus_tag="PXD71_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL6291058.1"
                     /translation="MTPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGH
                     ANIAGYDFIVIDTGGIDGTEEGVEEKMAEQSLLAIEEADVVLFLVDARAGLLPADVGI
                     AQYLRQREKTTVVVANKTDGIDADSHCAEFYQLGLGEVEQIAAAQGRGVTQLIEQVLA
                     PLGEQLNAEQFNQEQAGENAKNVANEETDEWDTEFDFENEEDAALLDEALAEEAEESI
                     EDKNIKIAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGQQYTIID
                     TAGVRKRGKVNLAVEKFSVIKTLQAIQDANVVLLTIEAREGISDQDLSLLGFILNAGR
                     SLVIVVNKWDGLSQDIKDQVKSELDRRLDFIDFARVHFISALHGSGVGNLFDSVKEAY
                     ACATQKTSTSMLTRILRMAADEHQPPLVNGRRVKLKYAHPGGYNPPIIVIHGNQVEKL
                     ADSYKRYLSNYFRKSLKIIGSPIRILFQEGNNPFAGKKNKLTPSQLRKRKRLMKFIKK
                     SKK"
     gene            8327..9754
                     /gene="hldE"
                     /locus_tag="PXD71_000914"
     CDS             8327..9754
                     /gene="hldE"
                     /locus_tag="PXD71_000914"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDL6291059.1"
                     /translation="MMMQYSPKFNNAKVLVLGDVMLDRYWFGATNRISPEAPVPVVKV
                     QDIEERAGGAANVAMNIASLGVPVTLHGLIGDDDAGRALDKLLNSHQIQNHCVVINSH
                     PTITKLRILSRHQQLLRLDFEEGFHNVDSTALLAKLSQEITAYGALILSDYGKGTLES
                     VQQMIQIARNAKVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGHCKDEDEIVSKGL
                     KLIADYDLSALLVTRSEKGMTLLRPNHEPYHLPTQAKEVYDVTGAGDTVISVLATAIA
                     DGRAYEEACYLANAAAGVVVGKLGTSTVSPVELENAIHQRTATGFGVVSEAELKAIVE
                     EAKKRGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNTDASVKRLKGEARPIN
                     DLAARMAVLAGLASVDWVVPFDEDTPQRLISEILPDLLVKGGDYKPEEIAGSQEVWAN
                     GGDVRVLNFENGCSTTNVIKKIQAL"
     gene            9768..10322
                     /gene="pyrR"
                     /locus_tag="PXD71_000915"
     CDS             9768..10322
                     /gene="pyrR"
                     /locus_tag="PXD71_000915"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL6291060.1"
                     /translation="MAREKIIIDTEQFQRTISRISHQIIEKHGDLQDVVIVGIKRRGA
                     EIAELIQKRIEDLAQTRLPYMALDITFYRDDLDLIYQDPVFMGADNQLNLNGKKVILV
                     DDVLFTGRTIRAALDALLDFGRAARIELVIFVDRGHRELPIRADYVGKNIPTAKNEQI
                     QVQTLSYDGINQVVLVPAANKESA"
     gene            10395..11345
                     /locus_tag="PXD71_000916"
     CDS             10395..11345
                     /locus_tag="PXD71_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL6291061.1"
                     /translation="MKMKLVKSLFAVAVATMSLTQTAHAFEERVVALVNDTPIMQSQV
                     QRVLGKKKATETAQRAAIDQVIDDMLVQQAMKEAGVKVSPAAVNQAVENVAIQNGITY
                     GQLLDALDYQGITLEQFKRNIAQQMAMEQVRHISISKSIQVAPQQVQALAKELMAKDK
                     AAGKLKTVSGKEHRISHILLKTNPILNDAQAKAKLSSLTTDINSGKISFEEAAKTHSV
                     DYASGADGGDLGFNFLEIYDPAFANVASKIKPNQISAPFKSQFGWHILKVTDTRNGDR
                     TEDAYHQRAYQQLVNKQAEEASKDWVKALRKTANIQYVGQ"
     gene            11407..12273
                     /gene="rsmA"
                     /locus_tag="PXD71_000917"
     CDS             11407..12273
                     /gene="rsmA"
                     /locus_tag="PXD71_000917"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL6291062.1"
                     /translation="MSSTNSKKHLGHTARKRFGQNFLHDMNIIHSIVSAINPKNGQFL
                     LEIGPGLGALTEPVAELVDKLTVVEIDRDLAERLRHHPFLNQKLTIIEQDALRFNFRD
                     YFNQLNLDEDSVRVFGNLPYNISTPLIFHLLTFHDLIQDMHFMLQKEVVKRLCAAPNS
                     KAYGRLTIMAQYYCQVIPVLEVPPTAFKPAPKVDSAVVRLVPYNQLPYPAKDVYWLNR
                     VTTQAFNQRRKTLRNALSTLFSAEQLDALGVDLTARAENLTIADYVRLANWLHDNPPA
                     IDTTEELVDEDC"
     gene            complement(12384..13013)
                     /gene="upp"
                     /locus_tag="PXD71_000918"
     CDS             complement(12384..13013)
                     /gene="upp"
                     /locus_tag="PXD71_000918"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL6291063.1"
                     /translation="MKIVEVKHPLVKHKLGLMREAEISTKDFRELANEIGSLLTYEAT
                     KDLEMESVEINGWSGEKIAVERIKGKKVTVVPILRAGLGMMDGVLEHIPSARISVVGI
                     YRDEETLKPVPYFSKLANDVDERLAIITDPMLATGGSMVATIDLLKKAGCKQIKVLVL
                     VAAPEGIKVLEAAHPDVELYTASIDSHLNENGYIIPGLGDAGDKIFGTK"
     gene            complement(13038..14723)
                     /gene="fadD"
                     /locus_tag="PXD71_000919"
     CDS             complement(13038..14723)
                     /gene="fadD"
                     /locus_tag="PXD71_000919"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDL6291064.1"
                     /translation="MEKIWLNNYPPYSPKTIETDQYESLVEMFEAAIQRHPDIPAYIN
                     MGQVLTFRKLEERSRSFAAYLQNELRLKKGDRIALMMPNLLQYPIALFGALRAGLVVV
                     NVNPLYTPRELEHQLNDSGAKAIVIVSNFAATLEKIVFNTEIKHVILTRMGDQLSFGK
                     RTLVNFVVKYIKKLVPKYKLPHAVSFREALSIGKQRQYVKPNIVSQDLAFLQYTGGTT
                     GVAKGAMLTHQNLIANMMQAKWIVEPLLGNSANMIGLVPLPLYHVLALSMNCLLFIEL
                     GLTGLLITNPRDIPGFIKELKKYKVSVITGVNTLFNALLNHESFKEVDFSHLKLSVGG
                     GAAIQSSVAKRWHDLTGVHIIEGYGMTECSPLISATRGDSVEYSGSIGVPVPNTDIRI
                     VDDEGKEVPLGERGELVVKGPQVMQGYWNRPDETAQVLQDGWMATGDIVVMGDDLNLR
                     IVDRKKDMIIVSGFNVYPTEIEEVISQNPKVNEVVAIGVPSEASGESIKIFVTKKDES
                     LTRDELRTYCRQFLTGYKIPRDIEFRDELPKSNVGKILRRVLRDEELAKVNKA"
     gene            complement(14701..15165)
                     /locus_tag="PXD71_000920"
     CDS             complement(14701..15165)
                     /locus_tag="PXD71_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDL6291065.1"
                     /translation="MMIEQATVVEYQNGVAIVQCYAKSSCGGCVAQSACGSKALSALA
                     GEKIDPRFRIEVDEPLKVGDKVELGLAENILLKSVFLIYGIPLLVLVMTAVGFSQFFA
                     NELIVLFIMLLSTGITFWAVRKLIHKHSKQANFSPIFLGKVLGDYNGKNMAK"
     gene            complement(15162..>16093)
                     /locus_tag="PXD71_000921"
     CDS             complement(15162..>16093)
                     /locus_tag="PXD71_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HDL6291066.1"
                     /translation="YTFAWAESTIKTPLAYLTAMSEAHKKSTYELVYILQQGSDTESF
                     RLRHAFTHNKEYAQLLNLDHSREEIILKNNKVSYLGYNFRPFSLNSSQILDNLPNVLY
                     TDYANLKGYAFLDAGKDRISDRITKVIRMKPNDTLRYAYILWIDEETHLLLKSQVLNM
                     DNLVLEEFRVLQLYQSPELDRIANVIESLMLPTLTTVKKEEFPKSDGWQVKWLPTGFR
                     LIQQQTVTGATYQVESEYVDSRVYSDGLSTLTIYIMPSQGVSFNEYAWQQGKLTILNQ
                     TINDRDIVIIGDVPLQSAKQIMNNIGFKEGAQP"
BASE COUNT         4656 a         3498 c         3228 g         4711 t
ORIGIN      
        1 taggagtata aatgaataac tacctactta aattaaaacc aatgacagcg gtcatacttt
       61 cgcttttgac ggtctttatc ttaggtttta caatgattgg gcttggctgg gtgcctcaaa
      121 tttcaattct agccgtgatt gtaatgctgc tacttttcgg cttaatcaat aaaattccgt
      181 ttaaaaatat gcaagagggt atggcaagcg ggctgatgtc cagtattggt gcggtttacc
      241 tattcttttt tattggatta ttggtttcag ctttgatgat gtctggtgcg attccaacct
      301 taatgtatta tggcttaagt gtgatttcac ctgaattatt ttacatttct gcattcgcat
      361 taagttctgt agtaggtatt gcgttaggca gtagtttaac cacaaccgcc accatcggtg
      421 ttgcttttat cggtatggct tctgctttcg gtgcgaaccc tgcgattgta gcaggtgcgg
      481 tggtttcagg ggcgtttttt ggcgataaaa cctctccact ttcagacact actaccattg
      541 ctgcttccgt agtgggcatt gatatgttcg ctcatatccg taatatgctt tataccacca
      601 tccctgcgtg gattttaaca gcattattcc tttggttttt aaccgaacct actgatgcac
      661 aaaatttagc aaatatagag aacctaaaaa ccaatttgca agaaacagga ttaatacacg
      721 gctactcttt gctacctttt gttacgttaa ttttaatggc aattaagcgt gttaattcaa
      781 tttatattat tgtaactaca gtaacactct cgctaattat tacctatatc cacagttcgc
      841 caacccttga acagctaggg aattatttct ttaccagcta taaacctgtt gaaggcgtag
      901 atttaggcga agtggcatca ttagtctctc gtggtggcat taatagtatg ttctttacca
      961 tcactattgt attgcttgcg ttaagtttag gtggtctttt acaaatcctc ggcattttac
     1021 cggcactact taacggcatt agcggctttt taaccagcac aggacgtgtt actcttgtag
     1081 ttgccagcac tgcggtggga attaacgtct taatcggtga gcaatatttg agcttattgt
     1141 tatcaggtaa tactttccgc tcaacttatg aacgtttagg cttagaagtg aaaaacttat
     1201 ctcgcaccat tgaagatgca ggcacggtaa tcaacccatt agtcccttgg ggcgtgtgcg
     1261 gtgtgtttat cagtagcgta ttaaatgtac cggtgttaga ttatctacca tacgcattct
     1321 tctgttatat ctgtttaatc cttaccttaa tttttggatt taccggggta acaatcagta
     1381 aaaaagctaa ctaatatgtg aaaaatgcct acaaatttgt aggcattttc ttttcattag
     1441 agctgaagaa cagcttgttt ggctaactct ctggcgagtt tatcaatatg caatacatca
     1501 gcaatttcac tgacttgaat tggagcaata ttttccacca ccgttctatt cacgttcaca
     1561 atatccgtaa agcggatttg ctcgtttaaa aacgcctcaa cggcaatttc attggcggca
     1621 ttcatcgctg ttgtcgcata ctgtccttct gcaaaagcct caatcgctaa ttttaagttc
     1681 gggtaacggg caaaatccgg ctcgataaag gttagctctt ttaatttaaa gaaatctaat
     1741 ggtggcacac cagcattaat tcggtttgga tatgccatcg tgtgagcaat cggcgtacac
     1801 atatccgggt tgcccatttg agcaatcacc gagccatcaa tataacgcac catcgaatga
     1861 ataattgatt gtgggtgaat gatgatatcc atttcctcgg ctgttgcatt aaacaaccag
     1921 cgggcttcaa tatactccaa gcctttgttc atcattgtgg ctgaatcgac tgaaattttc
     1981 ttacccatag accaattggg gtgagcaact gcttgagccg gtgtaatcgc atcaaattca
     2041 tttaatggct tagtacggaa cggtccaccc gatcctgtta aaataatttt actgacaccc
     2101 agttcagcga gtggacaaaa cccgactttt tgctgtgcct ccggtggaag cgattggaaa
     2161 atcgcattgt gttcactatc taccggtaag agcttcgcac cggatttacg agcctcatca
     2221 ataaaaattt gcccacaggt taccagcgat tctttatttg ccaacaatac ggttttaccc
     2281 gctttcaccg cagaaagtgt cggtaataac cctgctgctc ctacaatagc agccattacc
     2341 atatccactt cggaatgtgc agcaagctca caaatagctg cttgaccggc taacacttca
     2401 gttttagacc ccaaagttgc caatgccgtt tttaatgctt gtgcggattt ttcatcatcc
     2461 attgccgcaa aagtcggctg atattgacga cattgctcca gcattaattc aacattcttt
     2521 ccaccaacta acgcaaacac ctcatattgg tttgggtttg ggttatgctc aactaccgat
     2581 aaagtgcttt taccgataga acctgttgaa cctaaaatca ctaatttttt cattttgttt
     2641 ctctttatct aatgactgat aagggcgaaa gatttttcgc ccctacgccg taattatgcg
     2701 gatactcatt cgttgtttgt aaattgttta gcaaaaacaa ccgcttgtag attaactcga
     2761 aagccgtttt gcttcgataa catcatctaa ttgagcgata cgagccaata atttattcaa
     2821 aatttcaacg ttgttaagct gaatttccat atcaatggtg gcaaggctac gtttcacatc
     2881 ggttcggctg gaaacgccta acacgtttac tttttcattt gccataattg cactgacatc
     2941 tcgcagtaag ccgttgcggt cattggcaat cactcgaatg gttaagctta aaccagaggt
     3001 atagccttcg ccccaatccg cctccacaac tcgttctggg ttgagacttt gtaattcaaa
     3061 cagttgctca caatctgcac ggtgaatcga aatccctctg ccttgtgtga tgtaacccac
     3121 gatttcatca cccggaatcg gctggcagca acgggcaatg gaatgcatta agttccctac
     3181 gccctctaca atcacatagc ctttgcttgg cttcggctga ttggtgtttt tattcacctg
     3241 tttaagtacc gcttcgtctt cttgttcggc ggtcggttta attaattttg attgcaagaa
     3301 gtgcattaac tgatgaagac gaatatcgcc accgccaata ccggcataga gatcatcaaa
     3361 ctgttttaag ttatagcgag gcagagcata ttcttcaatt tgcttgagcg taaaaccgaa
     3421 tttttccatt tcggcttcaa gggcctcttt gccgattgga atatttttct ctcggtctaa
     3481 ttttttaaac cacgcaataa tttttgagcg agctttgcct gtattcacaa aaccggtatt
     3541 cggattgagc caatctcggc ttgggttcgg ctctttttgg gtgataattt caacctgatc
     3601 gcccatttgc agatgataag taaacggcac aatccgccct gccacttttg ctccaatgca
     3661 acgatgcccg atttcactat gaattgcata agcaaaatca agtggtgtag ctttttgcgg
     3721 taaatcgatc acctcgcctc ttggggtaaa gacataaaca cgatcatcaa aaatttgcga
     3781 acgcatttct gccaccatat cgcctgaatc ggcaagatcg ttttgccact caagcaattt
     3841 gcgtagccac acgatttttt cttcgtagcc cgaacgccct gcggttgccc cctctttata
     3901 tttccagtga gcggctacgc ctagctccgc atcatcgtgc atttgctggg tacgaatttg
     3961 cacttcaatc gttttttcac cttcgcctaa tacaactgta tgaatagact gataaccgtt
     4021 aggttttggg ttagcaatat aatcatcgaa atgttccggc aaatgggtat atttggtgtg
     4081 aataatgcct aatgccgtgt agcaatcttc taacgttggc actattaccc gcacagcccg
     4141 aatatcgaaa agctgattaa atctcaaccg ttttttctgc attttcttcc aaatgctgta
     4201 gatatgtttc gggcgtccat aaatctgcac attcttgatt tcaggactta aacaagcggt
     4261 cagatctgct acaaaatttg caatataacg ctcacgatcc acacggcgtt cgcccaattc
     4321 aaatttagca atttgagtgt aagagacagg gtgtaaaatg cggaaacagt aatcttccag
     4381 ctcccatttg agctgcccga tacccaatcg attggcaagc ggtgcataaa cgtgagaaca
     4441 ttctttagcg gcgagaacgg tatcttcttc ggaatggtgg ttactatcac gcaggtaggc
     4501 aattcgttcg gcaagtttaa tcaccacaca gcggaaatcg tccaccatcg ccaacaacat
     4561 gcgacggata ttgtcgattt gcagatccga cacattattg gcactaagct ggcggatatt
     4621 ctccatttcg agtacgcctt taactaagtt tttgatgtcg ttactgaaat cttcttttaa
     4681 ttgcaccaag tcgatcacat tgtgttttac cagaggaaaa agcattgcgg caactaagct
     4741 gtcatcgtcc atattcaagc cgtgcaatac ttcaaccatc tcaatgccat accacatcag
     4801 actatagtcg gtatccagtt tttcctgtgc ataactccac gcattgtgaa tctgctcaaa
     4861 agtcacagga gacatattca ggcttgcaca ccaactcgac aactcaaaat tattcggttc
     4921 aagttgatgg gaataacgaa ttgctaccat aaggcctcct tgtaaatttt tgtggaaatt
     4981 agaccgcttg tacagcttaa aatggagttt tattatacgt ggaaattcag aaaaaattta
     5041 cccctaatcg aaaatttaat ccaagttatc ttatcctctt tactccttaa gaggtagcaa
     5101 ccttgtgata aatcaaacct atcaacacaa tcatttgcac gacttcacgc tcttgattaa
     5161 aatacttgcg tttttatttt gatctttatt tttgtgggta gcaatgacaa ttttagcttt
     5221 aggaatcaac cataaaaccg cctctgtggg tttaagagag aaagtggcgt ttgtagatga
     5281 caaacgtaaa ctggctcttg aacaaatcca aacgagcgga ttggctgaaa gcgtggtcat
     5341 tctctctacc tgcaaccgca ccgagctcta cttccaccag cctaatattt ctccccgtga
     5401 agagagcgag gaaaatatcc aatggcgtga acaatgtttc cgctggtttg cccaaattca
     5461 tcaacttgat gaaagcgagt tgcgtgaatg tttatatttt aaacaaaatt tagaagccgc
     5521 taaccactta atgaaagtgg caagcggatt agattcacta attttaggcg aaccacaaat
     5581 tttagggcag gttaaacaag cctatcaata tagcgaagat ttctaccaac atcagcaaaa
     5641 agcgatgtcc accaagctat cacgcttgtt ccaacgcacc tttgccaccg caaaacgagt
     5701 acgttccgaa accgaaatcg gcagccaagc ggtgtcagtt gcttatgcag cttgcggttt
     5761 agcccgtcaa attttcgata attttggcaa gttacgcttt ttgctggtag gggcaggcga
     5821 aaccattgaa ttagtcgccc gctatttaat tcaacacggg gcgaaaaata taatgttggc
     5881 aaaccgcacc catattcggg cggaaatgtt ggcggaaaaa ctaaacgctc caatgcaaat
     5941 tctgtcgtta agtgctttgc aactcggttt aaaccaagct gatgtagtta ttagctccac
     6001 cggtagccct gataccctaa tcagtaaaga aatggtggaa attgcgcaaa aacaacgcca
     6061 atttgaccca cttttgctga ttgatattgc cgttcctcgt gatattgacg aaaaagccgg
     6121 cgacttagag ggcgtttacg cttatagtgt tgatgattta caaaatatta tccaacgcaa
     6181 tatggctcag cgtgaagaag ccgccaaaca agccgctgaa atcgtcaaag aggaaagcaa
     6241 agcatttttt gaatggttaa aacaacagca gtctagtgat ttaatcaaac gctatcgtca
     6301 agatgcggag caaacccgcc aagaattgtt ggagaaagcc ctacaagcaa tcagtcaagg
     6361 gcaagacagc caaaaagtat taagtgagct aagctacaaa ctcaccaacc agttactaca
     6421 cgccccaacc caagcgttac aagcaatggc aaaaagtggc aactccaccg gcttacaaag
     6481 tttttcaaga gcattacggc tagaagaatg atgctcagca cattctttta ggcatatttg
     6541 caaaaaattg aacattttta accgcttgcg gacgcacgcc gtgcgtccct ccccgtgttc
     6601 ccgatcttct tctaatttca acttccccaa atttccgcta taataagcca atttttgcaa
     6661 ctattttgca aataaccgga tatagaggat acaaaatgac tcctgttgtt gccctagtgg
     6721 gcagacctaa tgtcggcaaa tcgactttat ttaaccgttt aacccgtact cgagatgcct
     6781 tagtagcaga tttccccggc ttaacccgag accgtaaata tggtcacgct aatattgccg
     6841 gctacgattt cattgtaatc gacaccggtg gtattgatgg tacagaggaa ggcgtggaag
     6901 aaaaaatggc ggaacaatct ttattggcga ttgaagaagc cgatgtggtg cttttcttgg
     6961 ttgatgcccg agccggatta ttacctgccg atgtcggcat tgctcaatat ttacgccaac
     7021 gtgaaaaaac cacggtggtg gttgccaata aaaccgatgg tattgatgcc gactctcact
     7081 gtgccgaatt ctatcaatta ggcttaggcg aagtagaaca aattgctgca gcacaagggc
     7141 gtggggttac gcagcttatc gagcaagtgt tagctccttt aggcgaacag ctaaatgccg
     7201 agcaatttaa ccaagaacaa gcgggcgaaa atgctaaaaa tgttgcaaat gaagaaacag
     7261 acgaatggga taccgaattc gactttgaga acgaagaaga tgcggcatta ttagatgaag
     7321 ccttggcaga agaagcagaa gagtcgattg aagataaaaa tatcaaaatt gcgattgtcg
     7381 gtcgtccgaa tgttggtaaa tcaacactga ccaaccgcat tttaggcgaa gagcgtgtgg
     7441 tagtttatga tatgccagga acaacccgtg attctattta tattccaatg gagcgtgacg
     7501 gtcagcaata taccatcatt gataccgccg gtgtgcgtaa acgtggcaaa gtgaatttag
     7561 cggttgagaa attctcggta attaaaacct tgcaagccat tcaagatgca aacgtggtgc
     7621 tattaactat tgaggcccga gaaggtattt ccgatcagga tttatcacta ctcggcttta
     7681 ttctaaatgc tggtcgttca ttggtgattg tggtaaacaa atgggacggc ttgtcgcaag
     7741 atattaaaga tcaagtgaaa tcagagttag atcgccgttt agattttatt gattttgcca
     7801 gagtgcattt tatctcggca ttgcacggct caggcgtggg gaatttgttt gattcagtaa
     7861 aagaagccta cgcttgtgca acgcaaaaaa cctcgacctc aatgctgact cgcattttgc
     7921 gtatggctgc tgatgaacac caaccaccat tggttaatgg tcgccgagtg aaattaaaat
     7981 atgctcaccc gggtggctac aacccaccaa ttattgtgat tcacggcaat caggttgaaa
     8041 aattagcgga ttcttacaaa cgttatttaa gtaattattt ccgtaaaagt ttaaaaatta
     8101 tcggctcgcc aattcggatt ttattccaag aaggcaataa tccttttgcc ggtaagaaaa
     8161 acaaactgac accaagccaa ttacgcaaac gtaaacgttt gatgaagttt attaagaaaa
     8221 gtaagaaata atatctaacg atagcgcaag catccacgct tgtgctaaaa ctaataatgg
     8281 cacaagcgtg gacgcttgcg ccatcattta acagagaata ttaactatga tgatgcaata
     8341 ctctccaaaa tttaataatg ctaaagtgct ggtgcttggc gatgtaatgt tagaccgtta
     8401 ctggttcggg gcaaccaatc gaatttcgcc tgaagcccct gtgccggtgg tgaaagtaca
     8461 agatattgaa gagcgtgccg gcggtgcggc aaacgtggcg atgaacatcg ccagccttgg
     8521 cgtaccggta acattacacg gcttaattgg cgatgatgat gccgggcgtg ctttagataa
     8581 attacttaat tcccaccaga tccaaaatca ttgtgtggtg attaattcgc acccaaccat
     8641 cacaaaattg cggattcttt cccgccatca gcaattattg cggttggatt ttgaagaggg
     8701 ttttcacaat gtagattcta ccgccctact tgcaaaactt tcgcaagaaa ttaccgctta
     8761 cggagcatta attctctcgg attacggcaa aggcacattg gaatcggtgc aacaaatgat
     8821 ccaaattgcc cgcaatgcta aggtgccggt gctgatcgac ccgaaaggca cagactttga
     8881 acgctatcgt ggggcaacat tgcttacacc aaatatgtcg gaatttgaag cggtggttgg
     8941 gcattgtaaa gatgaggatg agattgtcag caaagggtta aaactgattg ccgactatga
     9001 tttatctgca ctattagtta cccgttcgga aaaaggaatg accttacttc gcccgaacca
     9061 cgagccttat catctgccga cccaagccaa agaggtttat gatgtaaccg gtgcaggcga
     9121 taccgtaatc agtgtattag ccactgcgat tgccgatggc agagcctatg aagaagcctg
     9181 ctatttagcc aatgctgccg ccggtgtagt cgtaggtaaa ttagggactt caaccgtcag
     9241 cccggttgag ctggaaaatg cgattcacca acgcactgcc accggttttg gcgtggtttc
     9301 tgaagccgaa ttaaaagcga ttgtggaaga agcgaaaaaa cgtggcgaaa aaatcgtgat
     9361 gacgaacggt tgctttgata ttcttcaccc cggtcacgtt tcctatttgg aaaacgcccg
     9421 taaattaggc gaccgattaa ttgtagccgt caataccgat gcttcggtaa aacgcctaaa
     9481 aggcgaagct cgcccgatta atgatttagc tgcccgtatg gcagttttag ctggtttagc
     9541 ctctgtagat tgggttgtgc cttttgatga agatacacca caacgtttaa tcagtgaaat
     9601 cctacctgat ttattggtaa aaggtggtga ttacaagccg gaagagattg ccggcagcca
     9661 agaagtatgg gcaaacggcg gcgatgtgcg agtattgaac tttgaaaacg gctgttctac
     9721 cactaatgtg atcaagaaaa ttcaagcact ataatggtaa taagcgaatg gcgagagaaa
     9781 aaatcattat tgataccgag caatttcagc gcacaatatc tcgtatttca catcaaatta
     9841 ttgaaaaaca cggtgatttg caagatgtag tgattgtcgg catcaaacgt cgaggagctg
     9901 aaattgccga gttaatccaa aaacgcattg aagatttagc tcaaacacgc ttgccttata
     9961 tggcgttaga tattacgttc taccgtgatg acctagactt aatttatcaa gatcccgtat
    10021 ttatgggagc cgataatcag ctaaatctga acggtaaaaa agtgattttg gttgatgatg
    10081 ttctgtttac cggcagaacc attcgggcgg cacttgatgc actcttagat tttggcagag
    10141 ctgccagaat tgaactggtg atttttgtcg atagaggtca tcgggaattg cctatccgag
    10201 ccgattatgt cggaaaaaat attccaacgg ctaaaaacga gcaaattcag gtgcaaacgc
    10261 tctcgtatga tgggattaac caagtggtct tagttcctgc tgccaataaa gaaagtgctt
    10321 aaattaagtg aaccttttcg ctaaacttat ctctaaggaa aagttactgc ttaattacct
    10381 ccattaggat tgttataaaa atgaaattag tcaaatcatt atttgctgtt gccgttgcaa
    10441 caatgagtct tacccaaacc gcccacgcat ttgaagaaag agtggtggcg ttagtgaatg
    10501 atacccctat tatgcaaagc caggttcaac gtgtgcttgg caagaaaaag gcgacagaaa
    10561 ctgctcaacg tgcggctatt gatcaagtga ttgatgatat gttggtgcaa caggcaatga
    10621 aagaagccgg cgttaaagtc agcccagctg cggtaaacca agcggttgaa aatgtagcaa
    10681 ttcaaaacgg cattacttac ggtcagttat tagatgcgtt agattatcaa ggtattacac
    10741 ttgaacaatt caaacgtaat attgctcaac aaatggcaat ggaacaagtt cgccatatca
    10801 gcattagcaa atcgattcag gttgcaccac agcaagtgca agctctggcg aaagagttaa
    10861 tggcgaaaga taaagcagca ggcaaattga aaaccgtttc tggcaaagag catcgcatta
    10921 gtcatatttt gctcaaaacc aacccgattt taaatgatgc tcaagccaaa gcaaaactta
    10981 gcagcttaac tactgatatt aactcaggca aaatcagctt tgaagaagcg gcaaaaaccc
    11041 attctgttga ttatgcctca ggggcagacg gtggagattt aggttttaac ttcttagaaa
    11101 tttacgatcc ggcatttgcg aatgttgcat caaaaatcaa accaaaccaa atttctgctc
    11161 cgtttaaatc gcaatttggt tggcatattt taaaagtcac cgacactcgt aacggagaca
    11221 gaaccgaaga tgcttaccac caacgtgctt atcagcaact ggtgaacaag caagccgaag
    11281 aagcctctaa agattgggta aaagcgctta gaaaaaccgc caatattcaa tatgtcggtc
    11341 aataattaaa atttttaaga acctcgcata atgcggggtt cttttcttat ttcaaaaagg
    11401 ataacaatga gttctacaaa ttccaaaaaa catttaggtc ataccgctcg taagcgtttc
    11461 gggcaaaact ttttgcacga tatgaacatt attcacagca tagtttcagc cattaacccg
    11521 aaaaacggac aatttttatt agaaatcggt ccgggtttag gagcattaac cgagccggtt
    11581 gccgaattgg tagataaact cacggtggtt gaaattgacc gtgatttagc agagcgttta
    11641 cgccaccacc cgtttttaaa ccaaaaactc accattattg agcaagatgc cctacgcttt
    11701 aatttccgtg actattttaa ccaactcaac ttagacgaag actcggtgcg tgtgtttggt
    11761 aacttacctt acaacatttc tacgcccttg atattccacc tgctgacctt tcacgactta
    11821 attcaagata tgcactttat gttgcaaaaa gaggtggtga aacgtttatg tgccgcgcca
    11881 aacagtaaag catacgggcg tttaaccatt atggctcaat actattgcca agtaattccg
    11941 gtgctggaag tgccgccaac cgcctttaaa ccggcaccaa aagtcgattc tgcggtagtg
    12001 cgtttagtgc cttataacca actgccttat ccggcaaaag atgtgtattg gctcaatcgg
    12061 gtcacgactc aagcctttaa ccaacgccgt aaaaccttac gcaatgctct ttcaaccctg
    12121 tttagtgcgg agcaactgga cgccttaggc gttgatttaa ccgcccgagc ggaaaacctt
    12181 accattgccg attatgtgcg tttggctaac tggctacacg acaacccgcc ggcaattgat
    12241 acaaccgaag aattggttga tgaagattgc taatcaataa attacaagcg gtcatttttc
    12301 taccaagatt tgcaaatctt tagtgaaaaa tggccgcttg tttaaatcag ctttacttgt
    12361 tttgctgtta cttagttttg atgttacttg gtgccgaaaa ttttatcccc tgcatcgcct
    12421 aagcctggaa tgatatagcc gttttcgttt aagtgactat cgattgaggc ggtgtagagt
    12481 tcaacatctg ggtgggcggc ttctaatact ttaatcccct ctggagcggc aactaacact
    12541 agcactttaa tctgcttaca gcctgctttt ttcaggaggt caatggttgc taccattgag
    12601 ccaccggttg caagcattgg gtcggtgata atggctaaac gctcatcaac atcgttggct
    12661 agtttggaga aatacggcac gggctttaag gtttcttcgt cacgataaat cccaaccaca
    12721 ctaattctgg cacttggaat atgctctaac acaccgtcca tcatacctaa gccagcacgc
    12781 agaatcggca caacagtcac ttttttgcct ttaattcgct caaccgcaat tttctctccg
    12841 ctccagccat tgatctccac actttccatt tccagatctt tggttgcttc gtaagtaagc
    12901 agactaccaa tttcattcgc tagctcacgg aaatctttgg tgctgatttc cgcttcacgc
    12961 attaagccca atttatgctt tacaagtggg tgttttactt caacaatttt cattttaagc
    13021 tccttaatta gtgtaattta agccttattc acctttgcta attcttcgtc tcttaacact
    13081 cggcgaagaa ttttgccgac attacttttt ggtaattcgt cacggaattc gatgtctctt
    13141 ggaattttgt agccggttaa gaattgacga caataggttc tcaattcatc tcgagttaaa
    13201 ctttcatctt ttttggttac aaaaatttta atgctttcgc cggaagcctc actcggcaca
    13261 ccaatcgcca ctacttcatt cacttttggg ttttgggaaa tcacttcttc aatttcggtt
    13321 gggtacacat taaaaccgga gacaataatc atatcttttt tacgatcaac aatgcgtaag
    13381 ttcagatcat cccccattac cacaatgtca ccggttgcca tccaaccatc ttgtagcact
    13441 tgtgccgttt catcaggacg gttccaatag ccttgcatta cttgcggccc tttcaccacc
    13501 agctcgcccc gttcgccgag tggtacttct ttgccttcat catccacaat acgaatgtca
    13561 gtatttggca ctggcacgcc aatagagccg gaatattcca ccgaatcgcc acgggttgca
    13621 ctaatcagcg gagaacattc tgtcattcca taaccttcga taatatgtac gccggttaaa
    13681 tcgtgccagc gttttgccac gctagattgg atcgctgcac cgccacctac acttaatttt
    13741 aggtgtgaaa aatcaacctc tttaaaactt tcgtgattaa gtaatgcatt aaacaatgta
    13801 tttaccccag taatcactga aactttgtac ttttttaatt ctttaataaa gcctggaata
    13861 tcgcgcggat tagtaattaa taatccggta agtcctaatt cgataaagag caagcaattc
    13921 atcgaaagtg ctaagacgtg atacagcggt aaaggtacca atccaatcat attggcagaa
    13981 ttacccaaca atggctcaac aatccatttt gcctgcatca tattagcaat taagttttga
    14041 tgagtcagca ttgccccttt cgctacacct gtagtaccgc cagtgtattg taaaaaggcg
    14101 agatcttggc tgacaatatt cggttttaca tattgacgct gtttgccaat gcttaaggct
    14161 tcacggaagc tgaccgcatg tggcaattta tatttcggca ctaatttttt gatgtatttc
    14221 accacaaaat tgactaaggt gcgtttgccg aatgaaagtt ggtcgcccat tcgggtcaaa
    14281 ataacgtgct taatctcagt attaaagaca attttttcta aggtagccgc gaagttcgag
    14341 actatcacaa tcgctttcgc accactgtca tttaattgat gttctaattc acgcggcgta
    14401 taaagcgggt tgacattcac caccacaagc ccggcacgta atgcgccaaa taaagcgatc
    14461 ggatattgca ataaattcgg catcatcaat gcgattcgat cacctttttt caggcgtaat
    14521 tcattttgca aataagcggc aaacgagcgg ctacgttctt ccaatttacg gaacgttaaa
    14581 acctgcccca tattaatata agctggaata tcagggtggc gttgtatcgc tgcttcaaac
    14641 atttccacta aagattcata ttgatctgtt tcaatcgttt ttggtgaata aggcggataa
    14701 ttatttagcc atattttttc cattatagtc tcctaaaacc ttacctaaaa agataggtga
    14761 aaaatttgct tgtttgctgt gtttatgaat caattttctt acagcccaga atgttatacc
    14821 tgttgaaagc aacattataa ataaaacaat aagctcattt gcaaaaaatt gcgaaaatcc
    14881 gaccgctgtc atcacaagaa ccagcagagg aatgccataa attaaaaaca cgcttttgag
    14941 caagatattt tcagctaacc ctagttcaac cttatcgcct acttttaggg gttcatctac
    15001 ctcaatacga aaacgagggt caattttttc acctgctaat gctgataatg ctttgctgcc
    15061 acaagcagac tgagctacac agccaccaca gctactttta gcataacatt gtacaatagc
    15121 cacaccattc tgatattcca ccaccgtagc ctgttcgatc atcatggctg agctccttcc
    15181 ttaaaaccaa tattgttcat aatttgctta gccgattgca acggcacatc accaataatc
    15241 acaatatctc tatcattaat cgtttgattt aaaatcgtta atttaccttg ttgccaagcg
    15301 tactcattaa aactcacgcc ttggctaggc ataatataaa tagttaatgt agataaacca
    15361 tcactatata ctctgctatc tacatactca ctttccactt gatatgtcgc acctgtgact
    15421 gtttgttgct gaattaacct aaaccctgtc ggcaaccact tcacctgcca accatcagac
    15481 ttaggaaatt cttctttttt cactgtcgtt aatgttggta acattagtga ttctattaca
    15541 tttgcaatcc tatccaattc tggagattga taaagttgca aaactctgaa ttcttctaat
    15601 accaaattat ccatattcaa gacttggctt ttaagtaata aatgtgtttc ttcatcaatc
    15661 catagtatat aggcatatct taaagtatca tttggcttca tacgaattac ctttgtaatt
    15721 cggtcagaaa tacgatcttt accagcatct aaaaaggcat aaccttttaa atttgcataa
    15781 tctgtgtaaa gtacattagg gaggttatct aaaatctgtg aactatttaa actaaaagga
    15841 cgaaaattat accccaaata gctgaccttg ttatttttta aaataatttc ttctcggctg
    15901 tgatctaaat tcagtaattg ggcatattct ttattatggg taaaagcatg acgtaaacgg
    15961 aaagattctg tatcacttcc ctgttgaaga atataaacta attcataagt ggatttttta
    16021 tgtgcttctg acatagccgt taaatatgct aatggtgttt taatagttga ttctgcccaa
    16081 gcaaatgtat agc
//
LOCUS       DANLXS010000039        16087 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.039, whole genome shotgun sequence.
ACCESSION   DANLXS010000039 DANLXS010000000
VERSION     DANLXS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16087
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.039"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..284
                     /locus_tag="PXD71_000922"
     CDS             <1..284
                     /locus_tag="PXD71_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291067.1"
                     /translation="YPNLDEWYSASDKERREFIAKHFAEETVRSDRNTPYDYAMCTGR
                     MFAKQLTRDEVKDYPLANTKPQYLTRDRRIEIMSNLPREILQNCAKYIQ"
     gene            complement(320..1072)
                     /locus_tag="PXD71_000923"
     CDS             complement(320..1072)
                     /locus_tag="PXD71_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL6291068.1"
                     /translation="MAYKPPFTVTTKMLNLVVEISHKLGTLQLDFERNLHLRKNNRLR
                     SIQASLAIEANSLTIEQVTAIIKGKKVLGHPKDIREVKNAYDAYDEMMHYNPYSIKDF
                     LKAHKFLTADLVNDAGAFRSKDVGIYNFSGQLVHMGARPQFVENLINELFSWAKQDDT
                     PALIKSSVMHYEIEMIHPFSDGNGRMGRLWQNVLLAQWNPLFAWLPIETMVYENQQRY
                     YQMLSLADSQNDSTGFIEFMLEMILETLLKLK"
     gene            complement(1111..2358)
                     /gene="clpX"
                     /locus_tag="PXD71_000924"
     CDS             complement(1111..2358)
                     /gene="clpX"
                     /locus_tag="PXD71_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDL6291069.1"
                     /translation="MAFEKEPHCSFCGKKRTEVDQLVEGTEGYICNECIEESYALLHG
                     EEEPLIDENQDDEGSFFTNVPTPHELHAHLDDYVIGQEHAKKVLSVAVYNHYKRLRSA
                     LSNHQATNGVELGKSNILLIGPTGSGKTLLAETLARRLNVPFAVADATTLTQAGYVGE
                     DVENVIQKLLMKCDFEAEQAERGIVFIDEIDKITRKSESASITRDVSGEGVQQALLKL
                     LEGTVANINPQGGRKHPKGETIPVDTSKILFICGGAFAGLDKIVEARTNTQGGIGFAA
                     ALKKDKDRQDLTELFKQVEPEDLVKFGLIPELIGRLPVVTPLQELDEEALIQILTEPK
                     NAIIKQYQALFEMEGVELHFTKDALISIAQKAISRKTGARGLRSIVENLLLDTMYDLP
                     TLNAKKVTIGKGCVENGDKPKVE"
     gene            complement(2358..2951)
                     /gene="clpP"
                     /locus_tag="PXD71_000925"
     CDS             complement(2358..2951)
                     /gene="clpP"
                     /locus_tag="PXD71_000925"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL6291070.1"
                     /translation="MALVPMVVEQTSKGERSYDIYSRLLKERIIFLNGQVEDNMANLI
                     VAQLLFLEAEDPEKDIYLYINSPGGVVTAGLAIYDTMNFIKPDVATLCTGQAASMGAF
                     LLSAGAKGKRFALPHARIMIHQPLGGARGQATDIQIQAEEILRLKATLTRRMAEHSGQ
                     SYEKVLADTERDNFMSAEEAAEYGLIDKVLTSRSEVA"
     gene            3063..3446
                     /locus_tag="PXD71_000926"
     CDS             3063..3446
                     /locus_tag="PXD71_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HDL6291071.1"
                     /translation="MTLTEIYQKFERCKSWEERYRLLIQLSRLLPKPSEQELAQIAEI
                     EGCESRLWFEFQAEPRKVVAYSDARLMQGILFIVSVALTEKTAEELKGFDLKTMFDEL
                     KISQNLTSTRLNGLGQLQKHIMMNL"
     gene            complement(3550..3626)
                     /locus_tag="PXD71_000927"
     tRNA            complement(3550..3626)
                     /locus_tag="PXD71_000927"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3590..3592),aa:Asp,seq:gtc)
     gene            complement(3715..4470)
                     /locus_tag="PXD71_000928"
     CDS             complement(3715..4470)
                     /locus_tag="PXD71_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6291072.1"
                     /translation="MKKSIYDTPIFFERYQQLRQNPISMNEVVEKPTMFSLLPDLTGK
                     KVLDLGCGTGVHLAHYLTLGAEKVVGLDLSELMLQQAESDLSAHWQKGNDFSLYCLPM
                     ERLNEISEGDFDVVTSSFAFHYIADFSELLAKISQKMTACGTLIFSQEHPIVTCYREG
                     YRWEKNQEKQQVAYRLNYYREEGERDRSWFQQPFKTYHRTMATIVNNLIQGGFEIVQM
                     EEPMLAGQPRWHNEFKDLQHRPPLLFIKAIKQK"
     gene            complement(4530..5525)
                     /gene="trpD"
                     /locus_tag="PXD71_000929"
     CDS             complement(4530..5525)
                     /gene="trpD"
                     /locus_tag="PXD71_000929"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDL6291073.1"
                     /translation="MQALLDKLFANQALAQAESHRFFSEVIKGNVSNEQLAAALIALK
                     LRGETVDEIAGAAKAALENANPFSTPDYPFADIVGTGGDGHNTINISTTSAIVAATMG
                     YKIAKHGNRSVSSKTGSSDVLSHLGINVDVSPETARLALDETNICFLFAPTYHSGFKY
                     AAPVRQALKTRTIFNILGPLVNPAHAKRQLLGVYSPELVKVYAETVKALGYEHTIVVY
                     GAGLDEVAVHGETQVAEIQNGQIDYYTLTPEDFGIERHSLEDLKGGEPAENAEKVTAI
                     LQGKGKLAHIDAVAANVALLMKTFGEDDLKANVQKVKETLESGKALETLRKLSTY"
     gene            complement(5543..5929)
                     /locus_tag="PXD71_000930"
     CDS             complement(5543..5929)
                     /locus_tag="PXD71_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HDL6291074.1"
                     /translation="MISVYGLKQTLADRRALIADVIFDCMQMSLGVPKQRHALRFDLL
                     DAENFYPPINRSQDFIVIEINLMQGRSEQLKKRLIKGLFSALHTKVGIVPMDVEITIK
                     EQPEHCWGFRGMTGNEATDLEYQAHR"
     gene            complement(5922..6512)
                     /locus_tag="PXD71_000931"
     CDS             complement(5922..6512)
                     /locus_tag="PXD71_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL6291075.1"
                     /translation="MANILFIDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLAKA
                     ETTENCLVALSPGPGNPAEAGNLLEIIRRLKDKVPMIGICLGHQALIEAFGGKVVHTG
                     TVLHGKVSRIEHDNQAMFSGINNPMLVARYHSLMGDELPAEFIVNAKFEHIVMAIRHK
                     TLPICGFQFHPESILTVQGTKLLKQSVEWLLGEKND"
     gene            complement(6522..8078)
                     /gene="trpE"
                     /locus_tag="PXD71_000932"
     CDS             complement(6522..8078)
                     /gene="trpE"
                     /locus_tag="PXD71_000932"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDL6291076.1"
                     /translation="MPQLTITQTELPYFADTTRVYYALCGERPHTLLLDSAEIQSKNS
                     VKSLLFAKSAVHIFCEDQTVTFHALTPNGEAVLPLIANALSLLNPAPTCKFLDKNDRL
                     QAIFPTISNQLDEDSKLKTTTVFDGLRTVSQLFADSTSPIYLGGLFSYDLVANFIPME
                     NISLQDDGLSCPDYTFYLAEQLVTIDHQTETAVLNTFCFEPNEQTNLNQQAVEFTQKL
                     ANLSHIELPLANQTATSEATANIEDEDFKAIIRKLKHHINIGDVFQIVPSRRFSLPCP
                     NTLASYKQLKINNPSPYMFYMQGENFTLFGASPESALKYSQANRQLEIYPIAGSRPRG
                     FDEQGNIDPELDSRLELELRLDQKELAEHLMLVDLARNDVARVSESGTRRVVDLMQID
                     RYSQIMHLVSRVIGTLRKDLDALHAYQACMNMGTLTGAPKIKAMQLIYQVEQQKRHSY
                     GGAVGYLTSDGNLDTCIVIRSAFVQNGIAYVQAGCGEVLDSDPQLEADETRHKARAVL
                     KAIGQVNAKA"
     gene            8220..8837
                     /locus_tag="PXD71_000933"
     CDS             8220..8837
                     /locus_tag="PXD71_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL6291077.1"
                     /translation="MTQFFYIHPDNPQQRLINQAVEIIRNGGVIVYPTDSGYALGCAM
                     GDKHAMDRIVAIRKLPENHNFTLVCSDLSELSIYALVNNQAYRLIKNNTPNPYTFILP
                     ATKELPRRLMTKRKTVGIRVPDNAIALALISALGEPILSCSLMLPETEMTESDPDEIR
                     DHLQNRVDLIIHGGYLGQQPTTVVDLTDDVPVIIREGSGDPTSFN"
     gene            8983..10050
                     /gene="rluB"
                     /locus_tag="PXD71_000934"
     CDS             8983..10050
                     /gene="rluB"
                     /locus_tag="PXD71_000934"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDL6291078.1"
                     /translation="MTTFQKKSTATFRADKRGESRPSFKKEEQKSERKFGKTSEKPPR
                     PTSKPLTQRIAKKVEKTTASEVASQEKKAKVVGEKLQKILARAGQGSRRELEEIIAAG
                     RVSVDGKMATLGDRVQVSSSTKIRIDGHLVNLMPAQKEICRVLMYYKPEGELCTRSDP
                     EGRSTVFDRLPRLSGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVR
                     VFGNIDDAMLQRLRKGVQLEDGPANFKQIKVVGGTGLNQWFDVTLTEGRNREVRRLWE
                     SQGVEVSRLIRIRYGNIKLDKGLPRGGWEEMGLEQVNYLRELVGLPPETETKVEVGVN
                     RRRTNIRQIRKAVKQHQKYRG"
     gene            10116..11888
                     /locus_tag="PXD71_000935"
     CDS             10116..11888
                     /locus_tag="PXD71_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDL6291079.1"
                     /translation="MSWDIVATLAVTGIAVFLFATEKMRMDVVAILVLCSLVLLGQVD
                     TKSALSGFSNSATVTVTAMFVLAAGLQNSGALDSVGSLLAKVKSPWLFLLVLCGVNAA
                     VSPFVNNTAVVAVLIPIVIAAAQNIKMAPSKALIPLSFSSQMAGVCTLIGTSTNLLVN
                     AIAQKQGHSGFGMFEFAPLGIIFLVVGVAYLLVTSRFLLPESHLQLEDGEGFGKYVSE
                     LKVNKDSVLIGKSTFESGLNEEFNLFTIGVLRDGERLSTPSHQVLQKHDILLLRGESE
                     NLAKVREKYGLHHVVYGRRENDEDNLDEDLMVAEVMISPISRWIGGTIPILQQRWNKN
                     ATVLGIQRQSKVIRERLRTTAFKMGDILLLTLPKEDMETLRQDKDFIVLSSDLVKTEE
                     SWKSKFALGVMVLVVATAALGWVPISVSALIGAVAMCVAGCLTAEEAYQSIDWKIILV
                     LAGLLPLGEAMANSGAAQFIVDNTLGKVGEFGPLVVLAVLYLLTMVLTEFMSNAGTAV
                     LLTPIAVSTAKMLGVDASPFIIAVMFAAATSFMTPVGYQTNTMVYGAGGYKFTDFIKI
                     GLPLNVIYWILGIVLIPIFFPFNP"
     gene            complement(11978..>16087)
                     /locus_tag="PXD71_000936"
     CDS             complement(11978..>16087)
                     /locus_tag="PXD71_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL6291080.1"
                     /translation="TWIVASELSRSANKSNATNTSLTTNIIKLSTLSLALSAGFASAA
                     TYSPTYGTIPDNNPQTSIAIGDGSKVNSTLVSTITGLEGNSGKVSTALGYNTTATADF
                     STALGGFANASFASTAVGGQATADKRSVAVGYNATAMGLREVFIGDHAGLNHSNSTEY
                     NIGIGYSASSNVTGNNTISIGNTAGDGTSGSHNIAIGTYANAKLAGPTTNVTSDDNIA
                     IGNSALANGVNNYKTTAATGETVIGKATAVGSHANATGIVSSAYGAEANATAIHSTAI
                     GAKSQANGDNSTAIGYEAKTSGHGATSLGYYANATANLTTAVGTNAGATSDYASAFGR
                     EANASGGSATALGNRATASGAASVALGVSAKATNQRTIAIGESSNASAFNATAIGRNA
                     TAEHTDSIALGSNSVTAIAIPTTNATVNGITYSDFAGTNPIATVSIGAEGKERTITNV
                     AAGRISLSSTDTINGSQLYLTQQAIGNVAATTANILGGGAAVTENGNITFPTYALVNG
                     TPDADKEGKQGRYTTVSAALSALNTAVISPLTFAGDTGTNFERHLGSTVKIKGGSTGI
                     LTENNIGVVADGNSTLTIKLAEKVNLGANGSLTTGDTVVNNTGITIANGVADKPVSLT
                     KSGLDNGGNKIANVAAGDVDTDAVNVSQLKQAISKFATHYVSISDDGIQRANYDNSGS
                     SGVNPMAIGVATSANGELATALGSEAEANGERTTAVGPRATADGMNATSIGYNANANA
                     TNALAVGSAANANADTSTAIGTASTATATRATALGSKSEATGENSTAVGYEASSIGAD
                     SLAAGYNANASGTQSTALGNSANAGGIWSTSVGRNANAAGSSAIALGNSANAAGVASI
                     ALGVSSQATTTAAVALGQNAKATHQGSVALGTNSETVATVATKSATLNGNTYTFAGTT
                     PSSTVSIGSVGNERTLTNVAAGRILDSSTDAINGSQLYAAYTEIDGLNTKVNELSNGA
                     LTFVDDAGTEIVRKLGTSLNVKGGADATILTDNNIGVVATDANTLTVKLAKDIDLTPA
                     GSVAVGNSKLNNNGLTINNGPSVTMTGVDAGKLKITNVADGDISPISADAVNGSQLYD
                     TANTIATALGGNSSVNANGAVSAPSYTVVDGAPTNEVSKTVNNVGSAITALNDAVTSP
                     LTFAGDTGTPSQRKLGSTVTVKGGVSNESQLTDNNIGVISNGNGSLTVKLAKDIKVNS
                     VTAQTVTANIAVADTVKTGDTTIDTNGLTIVGGPSITKTGINAAGTKVTNVKAGTEDT
                     DAVNFSQLKATEKNINNKINNIDSKVNKVDKRLRAGIAGATATAGLPQAYLPGKSMLA
                     TAGDTYRNEAAIAVGYSRISDNGKVIYKLTGNSNTRGDFGGSIGMGYQW"
BASE COUNT         4423 a         3283 c         3451 g         4930 t
ORIGIN      
        1 cttatcctaa tttagatgag tggtactcag cttcagataa agagcgaaga gaatttatcg
       61 caaaacattt tgcagaagaa acagtaagaa gtgatagaaa tacaccgtat gattatgcta
      121 tgtgcactgg tagaatgttt gctaaacaac tgactcgaga tgaagtcaaa gactaccctt
      181 tggcaaacac taagccgcaa tatttaaccc gtgatagacg gattgagatt atgtctaatt
      241 tacctcgtga aatattacaa aattgtgcta aatacataca gtaattttat gagtaacacc
      301 actttggaat taagtggtgt tattttaatt ttaataatgt ctctaaaatc atctccaaca
      361 taaactcaat aaagccggta gaatcatttt ggctgtctgc gagagaaagc atttgataat
      421 aacgttgttg attttcgtaa accattgttt caatcggcaa ccaagcgaaa agtgggttcc
      481 attgtgctaa aaggacattt tgccataatc gacccattct gccgtttcca tcactaaagg
      541 ggtgaatcat ttctatttca tagtgcataa ccgagctttt aatgagtgca ggtgtatcat
      601 cttgtttagc ccaagagaac aactcattaa tcaaattctc tacgaattga ggacgagccc
      661 ccatatgcac aagttgccca ctgaaattat aaatgccgac atcttttgaa cgaaaagccc
      721 ctgcgtcatt tactaagtca gcagtaagaa acttatgtgc tttgagaaaa tctttaatcg
      781 agtaaggatt atagtgcatc atttcatcgt aagcgtcata ggcatttttg acttcacgaa
      841 tatctttggg gtgtccgaga acctttttgc ccttaatgat tgcggtaact tgctcaatcg
      901 ttaatgaatt tgcctcaata gctaaagagg cttgaattga tctcaagcgg ttattttttc
      961 taagatgtaa attacgctca aaatccaatt gtagcgttcc caatttatga gaaatttcta
     1021 caaccagatt cagcatttta gtggtaactg taaaaggtgg tttataagcc attttgcaat
     1081 aatcctaaaa aattaaaccg cttgtcattg ttactccacc ttcggcttat cgccgttttc
     1141 aacacagcct ttgccgatgg tgactttttt cgcattgagg gttgggagat cgtacattgt
     1201 gtcaagtaat aagttctcga caatagaacg taacccacga gccccggttt tgcggctaat
     1261 cgctttttgg gcaattgaaa ttaatgcatc tttggtgaag tgcaattcaa cgccttccat
     1321 ttcgaataag gcttgatatt gcttgatgat cgcatttttc ggctcggtta agatttgaat
     1381 cagagcttct tcgtccaatt cttgcagcgg agtcaccacc ggtaaacgcc cgattaattc
     1441 aggaattaag ccgaatttga ctaaatcttc cggttcaact tgtttgaata gctcggttaa
     1501 atcttggcga tctttatctt ttttcaatgc cgctgcaaaa ccgataccac cttgagtgtt
     1561 ggtacgggct tccacgattt tatctaaacc ggcaaaagca ccgccgcaaa taaagagaat
     1621 tttggaggtg tccaccggaa tggtttcgcc tttcgggtgt ttgcgaccgc cttgtgggtt
     1681 aatgttcgcc accgtgcctt caagtagttt taataaggct tgttgaacac cttcgcccga
     1741 tacatcacgg gtaatggatg cactttccga tttacgggtg attttgtcga tttcatcaat
     1801 aaaaacaatg cctcgttccg cttgttcggc ttcaaaatcg catttcatta ataatttttg
     1861 aatcacgttt tcgacatctt ctcccacata gcctgcttgg gtaagggtag tagcatcagc
     1921 taccgcaaaa ggtacgttta aacggcgggc aagggtttct gccaacaagg ttttaccgct
     1981 accggtcggc ccgattaata aaatattact tttacctaac tctacgccat tagtggcttg
     2041 atggtttgac aatgcacttc ttaagcgttt gtagtggtta taaacggcca ccgaaagcac
     2101 ttttttcgcg tgttcttgac cgatgacata atcatctaaa tgagcgtgta attcgtgagg
     2161 tgtcggcaca ttggtgaaaa aactgccttc atcgtcttgg ttttcatcaa ttaacggctc
     2221 ttcttcaccg tgtaataagg cgtaggattc ctcgatacat tcattacaaa tatagccttc
     2281 cgtgccttcg actaattgat cgacttcggt acgtttttta ccgcaaaaac tacagtgggg
     2341 ttctttttca aatgccatta cgccacctca ctgcgagaag ttaatacttt gtcgattaag
     2401 ccatactcag cggcttcttc cgcagacata aaattatcac gctcggtatc ggctaacact
     2461 ttttcatagc tctgaccgct gtgttctgcc atacggcggg taagggtagc tttcaagcgt
     2521 aaaatttctt ccgcttgaat ttgaatatcg gttgcttgac ctctcgctcc acctaaaggt
     2581 tggtgaatca taatacgagc gtgcggcaga gcaaagcgtt taccttttgc cccggctgaa
     2641 agcaaaaatg cccccattga agccgcttgc cctgtgcaaa gggttgctac atcgggtttg
     2701 atgaagttca ttgtatcgta aattgccaag cctgctgtta ccacgccacc cggtgagtta
     2761 atgtataaat agatgtcttt ttccggatct tcggcttcta aaaacagaag ttgtgccaca
     2821 atcaggtttg ccatattgtc ttccacctga ccgtttaaaa aaataatacg ttcttttaat
     2881 aaacgtgaat aaatgtcgta agaacgttcg ccttttgagg tctgttccac caccattggg
     2941 actaatgcca ttttgttttc ctctgtcgta atacaaaatc tcgctattct agcaaatttt
     3001 cagaaaatca ttaacaacaa gcggtcagat tgttaaaatc ttttgcaaat gatgaggaaa
     3061 ttatgacatt aacagaaatt taccaaaaat ttgaacgttg taaatcgtgg gaagaaagat
     3121 accgcttgtt aattcagcta agtcgcttgt taccaaaacc aagtgagcaa gagttggcac
     3181 aaatagcgga aattgagggc tgtgaaagcc gtttatggtt tgaatttcaa gctgagccac
     3241 gaaaagtagt ggcatatagc gatgcccgtt taatgcaggg gattttattt attgtgagtg
     3301 tagctttaac tgaaaaaacg gcagaagagc tgaaaggctt tgacttaaaa acgatgtttg
     3361 atgagctaaa aatttcacaa aatttaacca gcacaaggtt aaatgggttg ggtcagttac
     3421 agaaacatat aatgatgaat ttgtaattca ccctcgcccc ttgtgggaga gggtgaataa
     3481 aatccgcagg attttattcg ggagagggga atataaaaaa cctctgattt ctcagaggct
     3541 taaatttgtt ggcggaacgg acgggactcg aacccgcgac cccctgcgtg acaggcaggt
     3601 attctaacca gctgaactac cgctccatac tggtgctaaa ataatagtgg tttcctcaac
     3661 attgagcaaa tagtttttgc aaaatagtgt ttaattggtt gtaaaataac cactttattt
     3721 ctgcttgatc gccttgataa acagcaacgg cggacggtgc tgtaaatctt taaattcatt
     3781 gtgccaccga ggttggcctg ccagcattgg ttcttccatt tgcacaatct caaacccgcc
     3841 ttgaataagg ttattcacaa tggttgccat tgtgcggtgg taggttttaa agggttgttg
     3901 aaaccaactt ctgtcccgtt cgccctcttc ccgataatag ttcaagcggt aagcaacttg
     3961 ttgtttttct tggtttttct cccaacgata gccttctcgg taacaggtga caatcgggtg
     4021 ttcttgcgag aaaatcagcg tgccacaagc agtcattttt tgcgaaattt ttgcaagtag
     4081 ttcggaaaaa tcagcaatat aatgaaacgc aaaagagctg gttactacat cgaaatcgcc
     4141 ctcgctgatc tcatttaatc gctccatcgg caggcagtaa agggaaaaat cgttgccttt
     4201 ttgccaatga gcagaaagat cgctttctgc ctgttgtagc attaattcag ataaatccaa
     4261 ccccaccact ttttctgcac cgagcgttaa ataatgggct aaatgaactc ctgtgccaca
     4321 acctaaatcc agcacttttt tgccggttaa gtctggcagt agagagaaca ttgttggctt
     4381 ttccaccact tcattcatac ttattggatt ttgtcgaagt tgttggtaac gctcaaagaa
     4441 aatcggagtg tcgtaaatgc tttttttcac ttaatagcct ttaaacaaat ataaaataag
     4501 gcgggttaaa acccgccact acgatatgat tagtaagtcg ataattttcg caaagtttct
     4561 aacgcttttc cactctcaag cgtttctttt actttttgca cattggcttt taaatcatct
     4621 tctccaaagg ttttcattaa taatgcgaca tttgctgcca ccgcatcaat atgagccagt
     4681 ttaccttttc cttgtaaaat agcggttact ttttcggcat tttctgcagg ttcaccgcct
     4741 ttgagatctt ctaacgaatg gcgttcaatg ccgaaatctt ccggcgttaa ggtgtaatag
     4801 tcgatctgtc cgttttgaat ttccgcaacc tgcgtttcgc cgtgaacggc aacttcatct
     4861 aaacctgcac cgtataccac aatagtgtgt tcgtaaccta aggcttttac ggtttcagca
     4921 tagactttca ctaactcagg ggagtacaca ccgagtaatt ggcgtttagc gtgagccggg
     4981 tttaccaacg gccctaaaat attgaaaatc gtgcgggttt ttaaggcttg gcgaacaggg
     5041 gcggcatatt tgaatccgct gtggtaggtt ggggcaaaca aaaagcagat gttggtttca
     5101 tctaaagcaa ggcgtgccgt ttcaggtgaa acgtcaacat taatacctaa atggcttaat
     5161 acatcgcttg agccggtttt acttgatacg ctacggttgc cgtgtttggc aattttatag
     5221 cccattgtgg cggcaacaat cgcacttgtg gtagaaatgt tgatggtatt atgcccatcg
     5281 ccacctgtgc cgacaatatc cgcaaaaggg taatcaggcg ttgagaacgg attggcattt
     5341 tcaagtgcgg cttttgcagc acctgcaatt tcatcaacgg tttcgccacg cagttttaga
     5401 gcgattaagg cagcagcaag ttgttcattt gatacattgc ctttaataac ttcactaaaa
     5461 aaacggtgag attccgcttg ggctaaggct tggttggcga ataatttatc taaaagtgct
     5521 tgcatagtct cttccttttt aattagcgat gtgcctgata ttccaaatcg gttgcctcgt
     5581 tgccggtcat tccacgaaag ccccagcaat gctctggctg ttctttaatg gtgatttcaa
     5641 cgtccattgg cacaatgccg actttggtgt gtaaggcact aaatagtcct ttaattaagc
     5701 gttttttgag ctgttcgctc cgcccttgca tcaggtttat ttcaatcaca ataaaatctt
     5761 ggctacggtt aattggggga tagaaatttt ccgcatccaa taagtcaaaa cgcaaagcgt
     5821 ggcgttgttt tggtacgcca aggctcattt gcatacagtc aaaaatcacg tctgcaatca
     5881 gggctctgcg gtctgctaag gtttgtttta atccatatac actaatcatt tttttctcct
     5941 aataaccatt ccaccgactg cttaagtaat tttgtgcctt gcacggttaa aatggattcc
     6001 gggtggaact ggaaaccgca aatcggtaag gttttgtggc gaattgccat cacaatatgt
     6061 tcaaatttcg cattaacgat aaattctgcc ggtaattcat cgcccattaa tgagtgataa
     6121 cgagcgacaa gcattggatt gttaatgcct gaaaacatcg cttggttatc gtgttcaata
     6181 cgagaaactt tgccgtgcaa tactgtgccg gtatgcacta ctttgccgcc aaaggcctca
     6241 attagagctt gatgtcctaa acaaatgcca atcatcggta ctttgtcttt cagacggcga
     6301 ataatttcca ataaattgcc tgcctcagcc ggattacccg ggccggggga aagggcaacc
     6361 aaacaatttt cggtggtttc agctttggct aaaaaatcct caagcggata atcattgcgg
     6421 aaaatcgtta cctgatgccc taattcacga aattgatcga ccaagttata ggtaaaagag
     6481 tcgaaattat caataaaaag aatatttgcc atagtttgtc cttatgcttt tgcattcacc
     6541 tgcccaatcg cttttaacac cgcgcgtgct ttatggcgtg tttcatctgc ctctaattgt
     6601 ggatcggaat ccagcacctc accacaacca gcttgcacat aggcaatgcc attttgaaca
     6661 aaggcggaac ggatcacgat acaggtatct aaattgccgt ctgaggttaa atagcccacc
     6721 gcaccgccgt agctatggcg tttttgttgc tcgacttggt agatcagttg cattgcctta
     6781 attttcggag caccagttag cgttcccata ttcatacaag cttgataagc gtgcagagcg
     6841 tcaagatctt ttctaagtgt gccgatcaca cgagaaacta agtgcataat ttgcgaatag
     6901 cggtcaattt gcattaaatc aaccacacgg cgagtgccgg attcgctcac acgagccaca
     6961 tcattgcggg caagatcgac taacattaaa tgttcggcaa gctctttttg gtctaaacgc
     7021 agttctaatt ccaaacggga atctaattca ggatcgatat taccttgttc atcaaaaccg
     7081 cgtgggcgag agccggcaat cgggtaaatt tccaactggc ggttggcttg cgaatatttc
     7141 aacgcacttt caggcgacgc cccaaacaga gtgaaatttt cgccctgcat atagaacata
     7201 tacgggcttg gattgttgat tttgagctgt ttatagctgg caagagtatt cgggcaaggc
     7261 aagctgaaac ggcgagaggg cacaatttgg aacacatcac cgatattaat atggtgtttg
     7321 agtttgcgga taatcgcttt gaaatcttca tcttcaatat tggcggttgc ctcactcgtt
     7381 gcggtttggt tcgctagagg cagctcaata tgtgacagat ttgcaagttt ttgcgtaaat
     7441 tcgaccgctt gttggttcaa attggtttgt tcattcggtt caaaacagaa ggtgtttagc
     7501 accgcagttt cggtttggtg gtcgattgta accagttgct ctgctaaata gaaagtgtaa
     7561 tctgggcaac ttaagccatc atcttgcagg ctgatatttt ccatcggaat aaaatttgcc
     7621 accaaatcgt agctaaataa tccgcccaaa tagataggcg atgtggaatc agcaaacagt
     7681 tggctcaccg ttcttagccc gtcaaacacg gtggtggttt tgagcttgct gtcttcatct
     7741 aactggttgc tgatggttgg gaaaatcgct tgcaagcggt cgtttttgtc taaaaatttg
     7801 caagtcggag cagggtttaa caggctcaat gcgtttgcaa tcaaaggtaa aaccgcttct
     7861 ccgtttgggg ttaaagcgtg gaaagtcacg gtttgatctt cacagaaaat atgcactgcc
     7921 gatttcgcaa acagcaaact ttttacactg tttttacttt gaatttccgc tgaatctaat
     7981 aatagcgtat gtgggcgttc gccgcataat gcgtaataaa cacgggtcgt atcggcaaaa
     8041 tagggtaatt ctgtttgagt aatcgttaat tgaggcatag caaattccta ttcaattttt
     8101 cgtactatta aactagtaca ttaaaaagaa gtcaagcaat tattccagat aattatgcga
     8161 gcttgggcga acttggtgta taattagccc aatttttaga aagtcgaagg cttgagacta
     8221 tgacacaatt tttttatatc caccccgaca acccacagca acgcttaatt aaccaagcgg
     8281 tagagattat tcgaaacggt ggcgtaattg tttaccctac cgattcaggc tatgctctcg
     8341 gttgtgcaat gggcgataag cacgcaatgg atagaattgt agcaattcgt aaattgccgg
     8401 aaaatcacaa ttttacctta gtttgtagtg atttatccga gctttctatc tacgcgttgg
     8461 tcaataatca agcctatcgc ttaattaaga ataatacccc gaatccttat acttttattc
     8521 tgccggcaac taaggaactt cctcgcaggc tgatgacaaa gcgtaaaaca gtggggattc
     8581 gtgtgccgga taatgcgatc gcattggcgt taatttccgc cttaggtgag ccgattcttt
     8641 cttgctcgtt aatgttgccg gagacggaaa tgaccgagtc agaccctgat gaaattcggg
     8701 atcatttgca aaatcgggtg gatttaatta tccacggtgg ttatttaggg cagcagccta
     8761 caacggtagt tgacttaacc gatgacgtgc cggttattat tcgagaagga tcgggcgatc
     8821 caacatcgtt taattaaatt gatgttgtat gattaatata tagaattatg cgtagggggg
     8881 ctgtgccagt ccgtattaat atcgttattt ggacaggcac aggacctgtc cctacaatga
     8941 aaatcaaatt cagacgcttg tgaaagcgac aaaaggaacc ttatgacaac ttttcaaaaa
     9001 aaatcaactg cgactttccg tgcagacaaa cgtggtgaaa gccgtccctc atttaaaaaa
     9061 gaagagcaaa aatctgaacg caaatttggt aaaacgtcag aaaaaccacc acgtccaact
     9121 tctaagccat taacgcaaag aattgcaaaa aaagtggaaa aaacgaccgc tagtgaagtg
     9181 gcatctcaag agaaaaaagc caaagtagta ggtgaaaagc tacaaaaaat cttagcgcga
     9241 gcagggcaag gttctcgccg tgaattagaa gagattatcg ctgctggacg agtgagcgtg
     9301 gacggtaaaa tggctactct tggtgatcgt gtgcaggtta gctcatcgac taaaatccgc
     9361 attgacggac atttagttaa tttaatgccg gcacaaaaag agatctgccg tgtattaatg
     9421 tattacaaac cggaagggga actttgcacc cgttccgatc cggaaggacg atccaccgta
     9481 tttgaccgct tgcctcgttt aagtggcgct cgttggattg cggtcgggcg gttagatatt
     9541 aacacctcag gcttattatt gttcactacc gatggcgaat tagccaaccg tttaatgcac
     9601 ccaagccgtg aagtcgaacg tgaatattcg gtgcgtgtgt tcggcaacat tgacgatgcg
     9661 atgttacaac gcttacgcaa aggcgttcag ttggaagacg gaccggcaaa ctttaaacaa
     9721 attaaggttg tgggtggcac aggcttgaac caatggtttg atgtgacttt aaccgaaggg
     9781 cgtaaccgtg aggttcgccg cctgtgggaa tcgcaagggg tagaagtcag ccgtttgatt
     9841 cgtatccgtt acggcaatat caaattagat aaaggcttac ctcgtggcgg ctgggaagaa
     9901 atgggcttag aacaagtcaa ttacttgcgt gagctggtag ggttaccgcc ggagactgaa
     9961 actaaagtgg aagtgggggt aaaccgccgc cgaaccaata tccgtcaaat tcgtaaagcg
    10021 gtaaaacaac atcaaaaata tcgtggctaa acaccatctt tggggctgca ttagttctgc
    10081 taatgcagct attttctttc aaaaaaggag catttatgtc ttgggatatt gttgcaacat
    10141 tggcggtcac tgggattgcc gtatttttat ttgcaactga aaaaatgcga atggacgtag
    10201 ttgcgatttt agtgctgtgt tcattagtat tattagggca ggttgatacc aaatctgctc
    10261 tgagtggatt ttcaaactca gctactgtaa ccgtaactgc aatgtttgta ttagctgctg
    10321 gtttacaaaa tagtggtgct ttagatagtg taggcagttt acttgctaag gtaaaatctc
    10381 cttggttatt cttgcttgta ctctgtggcg taaatgctgc tgtttctcct tttgtgaata
    10441 atacggcagt ggtcgcagta ttaattccga ttgtgattgc cgcagcacag aatataaaaa
    10501 tggctccttc caaagccctt attccgttat cattttcttc tcaaatggca ggtgtttgta
    10561 cactgatcgg cacttccact aatctattag ttaatgcaat cgctcaaaag caagggcatt
    10621 ccggtttcgg tatgtttgaa tttgcccctt tggggattat ttttttagtg gttggggttg
    10681 cctatttatt agtgaccagc cgtttcttat tacccgaatc acatttgcaa cttgaagatg
    10741 gtgaggggtt tggaaaatat gtttctgaac ttaaagtaaa taaagactct gtattaattg
    10801 gcaaaagtac atttgaatca ggattaaatg aagaatttaa tctatttact attggtgttt
    10861 tgcgtgatgg ggaaagatta tcgacaccga gtcatcaagt attgcaaaaa catgatattt
    10921 tattgttgcg gggtgaatcg gaaaatttag caaaagtacg agaaaaatat ggattgcacc
    10981 acgttgtgta tggacgccgt gagaatgatg aagataatct ggatgaagac ttgatggtgg
    11041 ctgaagtgat gatttctcct atttctcgtt ggataggagg gactattcct attttacaac
    11101 agcgttggaa taaaaatgca acggtgttag gcattcaacg ccaaagtaaa gtaattcgtg
    11161 agcgtttacg caccactgct tttaaaatgg gggatatttt attactcaca ttaccaaaag
    11221 aggatatgga gacgttacgc caagataaag actttattgt gttatcttct gatttagtca
    11281 aaacggagga atcgtggaaa agtaaatttg ctttaggggt aatggttttg gtggtggcaa
    11341 cagcagcctt aggctgggtt cctatttcag tgagtgcctt aattggtgca gttgcgatgt
    11401 gtgttgctgg ttgtttaacg gctgaagaag cctatcaatc tattgactgg aaaatcattt
    11461 tagtcttagc tggattactg ccattaggtg aagcgatggc aaatagtggg gcggcgcaat
    11521 ttattgttga taacacgctt ggtaaagtag gggagttcgg gcctttagtg gtgttagctg
    11581 tattatattt actgacaatg gtgttaaccg aatttatgag taatgcaggg actgccgtgt
    11641 tgcttacgcc tattgcggtt tctaccgcta aaatgttagg ggtagatgct tccccattca
    11701 ttattgccgt aatgtttgcg gctgcgacca gctttatgac ccctgttggc tatcaaacca
    11761 acacgatggt atacggtgca ggtggctata aattcacaga ttttattaaa attggtttac
    11821 cgctaaatgt aatctattgg attttaggga tagtattaat tccgattttc ttccctttta
    11881 atccataatt gatagataat tatagaaaaa aggtttacag atcagaaact ctgtaaacct
    11941 tttttattca gcaataaatc aattttctat tggaaaatta ccattgataa cccatgccaa
    12001 tactaccacc aaagtcgcct ctggtattgc tgttaccagt taatttatag ataaccttcc
    12061 cgttatctga aatccgagaa taacctactg cgattgcagc ttcattacgg tatgtatctc
    12121 cagccgttgc taacatactt ttacctggta gatatgcttg cggtaaacct gcggttgcag
    12181 ttgcaccagc aataccagcg cgtaaacgtt tatcaacttt gtttactttt gaatcaatat
    12241 tattgatctt attattgata tttttttctg ttgcttttaa ttggctaaag ttaactgcat
    12301 cagtatcttc agttcctgct tttacgttag tcacttttgt tcctgctgca ttaatacctg
    12361 ttttagttat actcggtccc cctacaatag ttaatccatt agtatcaatg gttgtatcac
    12421 cagtcttaac agtatcggct acagcaatgt tagctgttac tgtttgagct gtgacagaat
    12481 taactttaat atctttagct aatttaaccg ttaatgaacc attaccattt gaaataaccc
    12541 cgatattatt atcagttagt tgtgattcgt tagatacccc accttttact gtaacagtag
    12601 aacctaattt acgttgagat ggcgtgccgg tatcacctgc aaatgttagt gggctggtta
    12661 ctgcatcatt taacgcagta attgcagagc ctacattatt gaccgtttta ctaacttcat
    12721 ttgtaggagc gccatcaacc acagtatagc ttggggctga aacagcacca tttgcattta
    12781 cacttgaatt accacctaaa gcggtagcaa tagtatttgc cgtatcgtaa agttggctac
    12841 cattaactgc atctgctgag ataggtgaaa tatcaccatc agctacatta gttattttta
    12901 atttgccagc atcgacacct gtcattgtga cacttgggcc attattaatg gttaagccgt
    12961 tgttatttag ttttgaatta ccaacagcta cggagccggc aggagttaaa tcaatgtctt
    13021 tcgctaattt aactgttaat gtgttagcat ctgttgccac tacaccgata ttattatcag
    13081 tcaatatggt tgcatcggcc ccacctttaa cattcagaga agtacctaat ttacgaacaa
    13141 tttcagtacc agcatcatct acaaaagtaa gtgctccatt acttaactca ttcactttcg
    13201 tgtttaaacc atcaatttcg gtataagcag cataaagttg tgaaccgttg atagcatccg
    13261 ttgatgagtc caaaatacga cctgctgcaa cattcgttaa agtacgttca ttgcctacag
    13321 aaccaatact tacagttgag cttggcgtag tacctgcaaa tgtgtatgta ttgccattta
    13381 gcgttgcact ttttgttgca actgtggcta ctgtttcaga gttagtacct aatgcaacag
    13441 aaccttggtg tgttgctttt gcattttggc caagagccac tgcagcagtt gttgttgctt
    13501 gtgatgaaac acctaatgca atagaagcta caccggctgc atttgcacta ttacctaaag
    13561 caatcgcaga gctacctgca gcattggcat ttctacccac agaagttgac catattccac
    13621 ctgcattagc agagttacct aaagcggttg actgtgtacc cgaagcattt gcgttatagc
    13681 ctgcagcaag agaatctgca ccgattgaac ttgcttcata acctacagcg gttgaatttt
    13741 caccggtagc ttcagattta gagcctaatg cagttgcacg ggtagctgtc gctgttgaag
    13801 ctgtaccaat agctgttgag gtatcagcat ttgcattggc tgcagaacca accgcgaggg
    13861 catttgttgc atttgcattc gcattgtagc ctatagaagt ggcattcata ccatcagctg
    13921 ttgcgcgagg accaactgca gttgtacgtt caccgtttgc ttctgcttca gagcctaatg
    13981 cggttgccag ctcaccatta gcacttgttg caacgccgat agccattgga ttaacaccgc
    14041 tagagcctga attatcatag ttagcacgtt gaatgccatc atctgaaata ctaacataat
    14101 gagttgcaaa tttgctgata gcttgtttga gttgactgac attgaccgca tcagtatcta
    14161 catcgcctgc ggcaacattg gcgattttat taccgccatt atctaagcca gatttagtaa
    14221 gacttaccgg tttgtctgca acgccgttag cgatggtgat acctgtattg ttaacaacgg
    14281 tatcacctgt tgttaatgaa ccattagcgc ctaagttaac tttttctgct aattttatgg
    14341 ttaaagtact attgccatca gctacaacac caatattatt ctcggttaat atgcctgtag
    14401 atccgccttt gatcttgact gttgaaccta gatggcgctc aaaattagtt cctgtatcac
    14461 cggcaaaggt taacggactg atcaccgctg tgtttaatgc gcttagtgct gcagaaacag
    14521 tagtgtatct cccttgtttg ccttctttat ctgcatctgg tgtgccattc actaaagcgt
    14581 aggttgggaa agtgatattt ccattttcag taacagctgc cccaccacct aagatatttg
    14641 cggttgttgc agcaacatta cctatagctt gttgtgttaa atataattgt gaaccgttta
    14701 tggtatctgt agagcttaaa gatatacgtc ctgcagcaac attggtgata gttctctctt
    14761 tgccttccgc cccaatactt actgtagcaa ttggatttgt tccagcaaag tcactatatg
    14821 taataccatt tacagttgca tttgtagttg gtatggctat agctgtaact gagttgctac
    14881 ctagcgctat cgaatcggta tgttcagcag tggcatttcg acctatagcg gttgcattaa
    14941 aggcggaagc atttgatgat tctccgattg caattgtgcg ttgatttgtt gctttagcgc
    15001 ttacccctag tgcaacagaa gccgcgcctg atgcagtagc acgattgcct aaagcggttg
    15061 cagaacctcc acttgcattg gcttcacgac caaatgcaga agcataatcg ctggttgcac
    15121 ccgcattagt accaacagca gtagttaaat ttgctgttgc atttgcataa taacctaatg
    15181 aggttgcacc gtggcctgaa gtcttcgctt cataaccaat tgctgttgag ttatcgccat
    15241 ttgcttgaga ttttgcacca attgctgtgg agtgaatagc tgttgcgtta gcttcagcac
    15301 cataggcgga gctaacaata ccagttgcat tggcatgact acctacagca gttgcttttc
    15361 ctattaccgt ttctcctgtt gctgctgtag ttttgtaatt attaacacca tttgcaagag
    15421 cggaattacc gatggcgata ttatcatcac tggttacatt tgtagtaggt cctgctagtt
    15481 ttgcattagc ataagtacca attgcaatat tgtggctacc actagtaccg tctccagcag
    15541 tattaccaat agaaatcgta ttgtttcctg ttacattaga gctagccgaa taaccaatac
    15601 caatattgta ttctgtgctg ttagagtgat ttaatccggc atgatcgcca ataaaaactt
    15661 cacgtaagcc cattgcggtt gcattgtacc ctactgctac ggaacgttta tcggcagtgg
    15721 cttgcccacc aacagctgtt gaagcaaaac tagcgttagc aaagcctcct aatgcagttg
    15781 aaaaatcggc tgtcgcggtt gtgttatacc ctaatgctgt agaaacttta ccgctatttc
    15841 cttctaagcc tgttattgtg cttactagtg ttgagttaac tttagatcca tcaccaattg
    15901 cgatagatgt ttgtgggtta ttatctggta tggtcccata ggttggtgaa taggttgcag
    15961 cacttgcaaa acctgcagaa agcgcaagac ttagtgtgct gagtttaata atattagtgg
    16021 ttagtgatgt atttgttgca ttagatttat tagcacttct tgataattca gaagcaacaa
    16081 tccaagt
//
LOCUS       DANLXS010000040        15726 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.040, whole genome shotgun sequence.
ACCESSION   DANLXS010000040 DANLXS010000000
VERSION     DANLXS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15726
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.040"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(53..1093)
                     /locus_tag="PXD71_000937"
     CDS             complement(53..1093)
                     /locus_tag="PXD71_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6291081.1"
                     /translation="MQTTLSPQQIAKKHRTIEKKRSFLLLFFLLICLLSLIFDIMTGP
                     SLLPFNEVWKALFQLGEIEETTKVIVYELRLPMAIMALVVGAALGVGGAEIQTLLNNP
                     MASPYTLGLAAAAGFGASIVIAFGGFGLPTEISVPVGAFVMTLFSSAILFAFSSLGRF
                     SSAILVLVGIALLFLFQSLLSLVQFISAPEVSQQILFWLFGNLTKATWQTISIAFGVT
                     TVCILLLFKDAWKLTALRLGEARALSLGINLTWLRMKTLLIVSVMTATCISFVGIIGF
                     IGLVAPHIARMLLGEDQRFFLPGSMLVGAAFLSMSSVLSKSLVPGALFPVGIITALVG
                     VQFFFWIVLAKR"
     gene            complement(1182..1772)
                     /locus_tag="PXD71_000938"
     CDS             complement(1182..1772)
                     /locus_tag="PXD71_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HDL6291082.1"
                     /translation="MNFLTHSLISLEIDTQTKQQTLYGNFAGDFYKGLIPHLNLADNL
                     KSGVVLHRSIDKISDRPDSLTTQLLQGTFGRYKGIVSDIMIDHFLAKHFQRLFAKDLN
                     EVQAQIFQQIEQQRTIFPEQFVPMFNWLQSRKALTHYADLDFLEQVFLGMSSRVKKGE
                     ILRNAVTEIWQNYTLLEEAAIQEFFTVKDRATRELS"
     gene            complement(1773..3146)
                     /gene="modF"
                     /locus_tag="PXD71_000939"
     CDS             complement(1773..3146)
                     /gene="modF"
                     /locus_tag="PXD71_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDL6291083.1"
                     /translation="MVVDGNGSGKTTFSLALQGKISLYSGDYGNSFQHIQLLSFEQQQ
                     KMLEQIFKDRNNDSVSPDDFGLTARQVILNGSPKEALCEEYAHKLHIQHLLERPFMHL
                     STGESRKVLFCQMLVSEPDLFILDEPFEGLDQQSVQYWQQLIAELQSKMALVLIVNRF
                     NDIPDAANHIELLDNLSLILQGERAEIEQQAVYSQLKFAEENVNVPLPDSAAPLLSLP
                     ENINPFELENVNIQYGEKKILDNLSWTVQPKQHWWIKGPNGAGKSTLLSIITGDHPQS
                     YANKVRLFGKQRGSGETIWDIKKNIGYVSSQLHMDYRVNCSAMEVILSGFFDSIGVYQ
                     QVPNALQLKAMEWLERLNLSNVAKKPFRSLSWGQQRLLLITRAMVKHPSILILDEPLQ
                     GLDGINRKLVKQFIEQLVTNSQTQLLFVSHQDSDAPNCITHLFEFVPKGETNEFGFEY
                     HQTALGA"
     gene            complement(3264..4217)
                     /gene="fmt"
                     /locus_tag="PXD71_000940"
     CDS             complement(3264..4217)
                     /gene="fmt"
                     /locus_tag="PXD71_000940"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL6291084.1"
                     /translation="MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGK
                     KLQASPVKQLAEAHNIPVYQPKSLRKEEAQAELKALNADVMVVVAYGLILPEAVLNAP
                     KYGCLNVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMLMDVGLDTGDMLHKVTTPIEP
                     NETSASLYAKLAELAPLALLEVLNGLANHTFKAEKQDEALSNYAEKLSKEEAKLDWQL
                     PAVQLERNIRAFNPWPFSFLTLTVDGVEQSIKIHQATVLPHQEKAAGTVINVDKTGIQ
                     IATQQGVLNITQLQPAGKKPMSVQDFLNGRADWFVSGTQLS"
     gene            4332..4943
                     /locus_tag="PXD71_000941"
     CDS             4332..4943
                     /locus_tag="PXD71_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL6291085.1"
                     /translation="MEFFIPKADTIFEEEIKKSRFITYLRHTEGMEQAKAFWQELKIQ
                     HPHARHWCWATVAGLPNDSQQYGFSDDGEPSGTAGKPMLNYLLGSGLGEITAVVVRYY
                     GGIQLGTGGLVKAYGNGVQQALLQLERIRKVLRKNYRLQCEYEQFNTISHLISEKDIV
                     IVEQLFSDKVDLLLAFNPSEVEAFADELTQRFSGKLALIKEEI"
     gene            4947..6431
                     /locus_tag="PXD71_000942"
     CDS             4947..6431
                     /locus_tag="PXD71_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HDL6291086.1"
                     /translation="MQFFSIIRIVGILVMCFSFTMLVPATVALIYGDGGGRAFLEAFA
                     LNFMVGTLLWWLCRDNKNELRSREGFLIVVLFWVVLGLLGAVPFIILENPDLNVAESI
                     FESFSGLTTTGATVITGLDSLPKAILFYRQFLQWLGGMGIIVLAVAIIPLLGIGGMQL
                     YRAEMPGPLKEQKMRPRIAETAKALWLIYLSLTILCAVAFWLAGMTVFDAISHSFSTV
                     SIGGFSTHDASIGYFNNAAISYITVFFLLLSACNFALHFAVIDRIRDAQSRKKGGILS
                     RLYWGDYEFRFFLMVQLGLFLVCTLVLVGYGYFDNTSVTIEQALFQSVSISTTAGFTT
                     NDFSQWSSFLPTLLVLSSFIGGCAGSTGGGLKVIRVLLLYLQSKREIHRYIHPNVIQP
                     IKLGNHVLSERVVDGIWAFFSAYFFVFVICWLATIACGMETFDALNAVIASLNNLGPA
                     LGSVSSNFTQVPDSAKLVLTFAMVCGRLEVFTLLVILSPVFWKD"
     gene            6432..6959
                     /gene="hemG"
                     /locus_tag="PXD71_000943"
     CDS             6432..6959
                     /gene="hemG"
                     /locus_tag="PXD71_000943"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDL6291087.1"
                     /translation="MKTLILYFTTDGQTKKIAERLADVITHEVELISLKDQAVDFAEK
                     IANADQIVIGASIRYGHFNPLVYQFVEQHYPALNAKKTAFYGVNLTARKDNRNTPETN
                     TYVRKFLAKVKWKPTHIEVIAGALLYLRYKPFDRLMIRFIMKITKGETDTSKEYEYTD
                     WQQVENFGKLLANQL"
     gene            7091..7486
                     /gene="mscL"
                     /locus_tag="PXD71_000944"
     CDS             7091..7486
                     /gene="mscL"
                     /locus_tag="PXD71_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL6291088.1"
                     /translation="MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPP
                     IGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQNVFDFLIIALAVFGMVKVINSL
                     KKAPVVEEAPAEPTAEEKLLTEIRDLLKK"
     gene            complement(7599..7862)
                     /gene="rpsT"
                     /locus_tag="PXD71_000945"
     CDS             complement(7599..7862)
                     /gene="rpsT"
                     /locus_tag="PXD71_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL6291089.1"
                     /translation="MANIKSAKKRAVQSEKRRQHNASQRSMMRTFIKKVYAAVAAGDK
                     AAAQTAFVEMQKVVDRMASKNLIHANKAANHKSKLAAQIKKLA"
     gene            8126..9700
                     /gene="murJ"
                     /locus_tag="PXD71_000946"
     CDS             8126..9700
                     /gene="murJ"
                     /locus_tag="PXD71_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDL6291090.1"
                     /translation="MSKKLLRSGMIVSFMTLISRILGLIRDIVVATILGTGVSADIFL
                     FANRIPNFLRRLFAEGAFSKAFVPVLAEYNADNDPNKTREFIAKVSGTLGGLVTVVTL
                     VAMIASPVVAALFGTGWFLDWLYDGPNAEKFTQASFLLKITFPYLWFITFVALSGAVL
                     NTIGKFGVMAFSPVLLNVAIIAVALWGRDYFDSPDIALAWGVFLGGLLQFLFQIPFMK
                     KEGLLVKPKWAWKDEGVTKVRKLMIPALFGVSVTQLNLLLNQVIASFLITGSISWMYY
                     ADRLIEFPLGLFGIAISTVVLPSLSRIAKNKELTEIQRGEHFQNTMDWGVRMVLLLGI
                     PAMIGMAVLAQPIIMTMFMRGKFGFEDVLATSYPLWVMCLGLNSYMLISVLANGFYAN
                     QNTKTPVKVGIIAALSNICFGLAFAPFLGYIGLALASACSALVNVSLLYVNLSKNGYY
                     KVSHKTVLFVLKLFIAACVMGALVAYFTPEIELWALMTMWQKVYWLIWLMILAGGSYF
                     ASILVLGIRKKDFRSA"
     gene            9702..10169
                     /locus_tag="PXD71_000947"
     CDS             9702..10169
                     /locus_tag="PXD71_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDL6291091.1"
                     /translation="MKSLKLKIPPPAWFLLCAALMWLVAQYVPVSLPNYQHTAIFFSG
                     LAVAIIGGAVAVSALWVIHQAKTTYSPFRPQHTAKLVTWGIYRKSRNPMYLSLLLGLI
                     AWALWLGAMLAWLVLPLFVWLITYFQIKPEEQILAQKFGTHYAKYCNQVRRWL"
     gene            complement(10234..12243)
                     /locus_tag="PXD71_000948"
     CDS             complement(10234..12243)
                     /locus_tag="PXD71_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL6291092.1"
                     /translation="MDLIKKLNTGSTFRVPIFLPSLLFVSFVAVFCIIFPQQAQTSLD
                     TIKNSLFQHFSWFYIFAGSIFFLFLIFLSFSRLGDIKLGADTDEPEFGFGSWIAMLFA
                     AGMGIGLMYFGVAEPILHYLKPVQQNLTEPERMKEAMMTTFYHWGIHAWAIYGVIALA
                     LAYFGFRYKLALTIRSGFYPLLKHRISGFWGHLIDIIALCSTIFGLTTTLGFGVMQVS
                     AGFNNLGLIEQSNFTVLAIIVTVAMALAVLSAVSGVGKGVKILSEINLTLAGLLLIFV
                     IITGPTLLLFSSFTENLGYYFSSLLEMSFRTFAYEPEHQGWLSGWTVLYWAWWASWAP
                     FVGLFIAKISKGRTIREFILGVLFVPSLFNILWMTSFGSSAIWFDQQTAGALAEVSGN
                     TEQLLFTFFEQLPFGSIASFVAVIVISIFFITSADSGIFVLNSIASQGEENAPKWQSV
                     LWGALLAILALSLLYSGGLASLQTMTLIIALPFTFIMLILCIGLWKGLMVDNQYFNKK
                     FSQGSQHWAGKDWKQRLEKIINPSNKQDVRHFFIKVARPAFLELIEEFESYGLIAKMN
                     FTNEQNPKLEFEVVKENLRNFIYGIESVPRELSDLVVGDDNLPNIEQNTIYEPITYFL
                     DGRKGYDVQYMTKEELIADVLQQYERFINLAMDNSHDLMTADFNH"
     gene            12435..12970
                     /locus_tag="PXD71_000949"
                     /pseudo
     CDS             12435..12970
                     /locus_tag="PXD71_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12998..13609
                     /locus_tag="PXD71_000950"
     CDS             12998..13609
                     /locus_tag="PXD71_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6291093.1"
                     /translation="MKYEAKPLIISPNHIQQRLHGKLLARALLIIALLMLAKFIYIAS
                     LSNYFTFYLMEKFQLNIAMAQLSLFTFLGAVALGAFVGGPIGDKIGRNAVIWFSFLGM
                     APFALLLPHADLFWTIVCAIFAGFIMSSAFSAMVVYAQEAVPGRVGLISGTMFGLMFG
                     IGGLSAAVLGFFADEYGLESIFKACAYLPLLGFAALGLPKRNI"
     gene            complement(13620..14510)
                     /gene="rarD"
                     /locus_tag="PXD71_000951"
     CDS             complement(13620..14510)
                     /gene="rarD"
                     /locus_tag="PXD71_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL6291094.1"
                     /translation="MNISGKLQGWHYALLCYLTWGTFPIYWYPINQSGMPAEQILAQR
                     VLWSALFAVFLLLIFQQGRAVINAFKQPKVLGLFVLSSFMIGVNWLVYLWAIVNEHIL
                     DASLGYFINPIFNVFVGRLILKEQLNKAQLLALLFAISGILWLAIPAGQIPWVALILA
                     ASFCIYGLIRKLAPMEPLSGLALETLLMSPFAITYLCFCYHQNILVFSELNPLQMAVL
                     LCSGIATTLPLLWFAMGARRISMSLLGMLQYISPTLQFLCGVLIFNETLSAERAVGYI
                     LVWIGVGVFLLGMKKAGVKR"
     gene            complement(14592..15575)
                     /gene="epmA"
                     /locus_tag="PXD71_000952"
     CDS             complement(14592..15575)
                     /gene="epmA"
                     /locus_tag="PXD71_000952"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDL6291095.1"
                     /translation="MNNLQLDNIDWQPTASIPHLIQRSKIITEIRKFFTDRGLLEVET
                     PILSEFSVTDVHLSTFNTQFQSPFSPEAKTLHLMTSPEYHMKRLLAAGSGPIFQLCKV
                     FRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESFSYQFVFQTY
                     VGLDPLSATKAQLVEKARKHGLQCDDDEQRDTLLQFLFSEVVEANIGQERPTAVYHFP
                     STQAALAQISSEDHRVAERFEFYYKGLELCNGFHELDDADEQIHRFEQDNLQREKMGL
                     EPQQLDVRFLAALKAGFPNCSGVALGVDRLLMLAMDAEKIEEVISFGIEKA"
BASE COUNT         4411 a         3148 c         3478 g         4689 t
ORIGIN      
        1 ctaagtttcc acgcttaata gttacatcgt ttaagttgag catagaaaat ccttatcgtt
       61 tcgccaatac aatccagaaa aagaactgca caccgactaa tgccgtaata ataccgaccg
      121 gaaataatgc gcccggcaca aggctttttg atagcacgga tgacatcgat aaaaatgccg
      181 cacccactaa cattgaaccg ggcaggaaaa agcgttggtc ttcgcctagc aacattcggg
      241 caatatgcgg ggcaactaag ccaataaagc cgataatgcc tacaaagcta atgcaggtgg
      301 ccgtcattac agacacaatc agcaatgttt tcattcgtaa ccaagttaag ttaatgccta
      361 agctcaatgc tcgagcttcg cccaaacgca aggcagttaa tttccacgca tctttgaata
      421 ataataaaat acagactgta gttacgccaa aggcaattga gatagtctgc caagtagctt
      481 tagttaaatt accgaatagc caaaacagaa tttgttggga tacctcaggt gcagaaataa
      541 attgcaccaa tgacagtaaa gactgaaata aaaacaataa ggcaattcct accagcacta
      601 atattgccga gctaaaacgt cctaaagagg aaaaggcaaa taaaatggcg gaagaaaata
      661 gtgtcatcac aaaagccccg acaggtacag aaatttcggt gggtaaacca aatccaccaa
      721 aggcaatcac aatcgaagca ccaaagccgg ctgcagctgc taaacccaat gtataagggc
      781 ttgccattgg attattaagc aaagtttgaa tttcagctcc gccaacacca agtgctgctc
      841 caaccaccaa tgccattatt gccattggta agcgtagctc atagacaatc actttggtgg
      901 tttcttcaat ttcgcctaat tggaataagg ctttccaaac ttcattaaac ggaagcaggg
      961 aagggcctgt cataatatcg aaaatgaggc tgagtaaaca aatgagtaag aaaaagagta
     1021 ataaaaaact gcgtttcttt tcaatagttc ggtgcttttt agctatttgt tggggagaga
     1081 gcgttgtttg cataaaaatg taatgtttga gaattattat cagacaggat aagagaaaag
     1141 atcgataaat tcaagaatat ccaagatacc gactaaagta ttcaactaag ttctcgagta
     1201 gccctgtctt ttacagtaaa aaactcctga atagcggctt cttctaaaag agtataattc
     1261 tgccaaattt cggttacagc atttcttaaa atctcacctt tcttaactct tgatgacatt
     1321 cccaagaata cctgttctaa gaaatctaaa tcggcataat gagttaatgc ttttcgtgac
     1381 tgtaaccagt taaacatcgg cacaaattgt tcaggaaaaa tcgtgcgttg ttgttcgatt
     1441 tgctgaaaga tctgtgcttg tacctcattt aaatctttag caaataaccg ttggaaatgt
     1501 ttagcgagaa aatggtcgat cataatatcc gacacaatgc ctttataacg cccgaatgtg
     1561 ccttggagta attgggttgt gaggctgtcc ggtcggtccg aaatcttatc aatcgagcgg
     1621 tgtaacacca caccgctttt taagttatcc gctaaattga gatgcggaat taaaccttta
     1681 tagaaatcac cggcaaagtt gccatatagg gtctgttgct tggtttgggt gtcaatttca
     1741 agtgaaatca gcgaatgggt aagaaaattc atttatgctc caagtgcagt ttggtgatac
     1801 tcaaacccaa actcgttggt ttcgcctttc ggtacaaact caaataaatg cgtaatgcaa
     1861 tttggagcat cgctgtcttg gtgcgaaaca aaaagtagtt gcgtttggct gttagtcact
     1921 aattgttcaa tgaactgttt gactaatttg cggttaatgc cgtctaaacc ttgtaacggt
     1981 tcgtctagaa tcagaattga tgggtgtttc accattgctc gggtaatcaa aagtaagcgt
     2041 tgttgccccc acgagagaga gcgaaacggc tttttggcaa cattgcttaa attgaggcgt
     2101 tccaaccatt ccattgcttt taattgcaag gcattcggca cttgttgata cacgccgatg
     2161 ctgtcaaaaa agccagaaag aatgacctcc attgcggagc aatttacccg ataatccata
     2221 tgcaactggc tgctgacata gccgatattt ttcttaatat cccaaatggt ttcgccactt
     2281 ccccgttgtt tgccaaatag tcggacttta ttggcataag attgtgggtg atcgccggtg
     2341 ataatcgaaa gcaaggtcga tttcccagct ccattcgggc ctttaatcca ccagtgttgt
     2401 ttaggctgaa ccgtccaact taggttatct aaaatcttct tctccccata ttgaatattc
     2461 acattttcca actcaaacgg attgatgttt tccggcaggc tgagtagcgg agctgcacta
     2521 tcaggcagtg gcacattgac attctcttct gcaaatttta gctgagaata gaccgcttgt
     2581 tgttcaattt ctgctcgctc tccttgcaga attaatgata aattatctaa taactcaata
     2641 tgattggcag catcaggaat atcgttaaaa cgatttacaa tcagcactaa cgccattttg
     2701 ctttgtagtt cggcaatcag ttgttgccaa tattgcaccg actgctgatc cagcccttca
     2761 aacggttcgt ctaaaatgaa taaatccggc tcgctcacta acatttggca aaatagcact
     2821 ttgcggcttt caccggtgga aaggtgcata aacgggcgtt caagcagatg ttgaatatgc
     2881 aatttgtggg catattcctc acataacgcc tcttttggac tgccatttaa aattacttgt
     2941 cgtgcggtta agccgaaatc atcgggcgag acactatcgt tgttccgatc tttgaaaatc
     3001 tgctccaaca ttttttgttg ttgctcgaaa gagaggagct gaatatgttg gaacgaatta
     3061 ccatagtcgc cggaatagag cgagatttta ccctgtaaag cgagtgaaaa agttgttttg
     3121 cctgaaccgt ttccgtctac gaccacctaa aagtcattgg gttggatctg taaggcgtca
     3181 atcgtgagtt tatgttgttt aaccagtgaa aatcgggcat tggttattgt aagtgaaggc
     3241 tgtgtttgca tatatttccc gttttaactt agttgtgtcc cgctgacgaa ccaatccgct
     3301 cgaccgttta agaaatcttg caccgacatt ggttttttac ccgccggttg aagttgggta
     3361 atattcagca cgccctgttg ggtggcaatt tgaatcccgg ttttatccac attaattacc
     3421 gtgccggctg ctttttcttg gtgaggtaac accgtggctt ggtgaatttt gatactttgc
     3481 tcaacgccat caacggttaa ggttaaaaaa ctaaacggcc aagggttaaa agctcggata
     3541 ttgcgttcca gttgaactgc tgggagttgc caatccaatt tagcttcttc tttggaaagt
     3601 ttctcggcgt aattggacag tgcttcatct tgtttttccg ctttgaatgt gtggttggct
     3661 aaaccgttta acacctcaag caatgccaga ggggcaagtt ctgccagttt ggcatacaag
     3721 gaggcggagg tttcattcgg ctcaatcggg gttgtgactt tatgtaacat atcaccggtg
     3781 tctaagccaa cgtccattag cataatggtt acgccggttt ctgtatcacc tgcccaaatc
     3841 gaacgctgaa tcggagctgc tccacgccag cgaggtagta gcgaaccgtg tacgtttagg
     3901 caaccatatt taggagcatt caatactgct tcaggtagaa ttaagccgta agctaccacc
     3961 accatcacat cggcatttag ggctttcagt tcagcttgag cctcttcctt acgcagggat
     4021 ttgggttggt aaacaggaat gttgtgagct tcggccagct gttttaccgg gctggcttgc
     4081 agttttttgc ctcgcccggc aggtttatcc ggctgagtat aaaccgcaat cacgttatgc
     4141 tcggaatcta ataaggcttg taaatgttga gcggcaaaat caggcgtgcc ggcaaaaata
     4201 atattgagtt ttgacatatt ttttattcgt tcaagttatc aatgaaagac ttattaaacc
     4261 attaaacagc acaaataaaa agtggcttga atatggtatc attgccacat tcaagataag
     4321 agaatcaaac aatggaattt tttatcccca aagcggatac aatttttgaa gaagagatta
     4381 aaaaaagccg atttatcacc taccttcgcc atactgaggg tatggagcag gccaaggcat
     4441 tttggcagga gctgaaaatt cagcatccgc acgcacgcca ttggtgttgg gcaacggtgg
     4501 caggcttgcc gaatgattcg caacaatacg gcttttcgga tgatggagaa ccgtctggta
     4561 cagcaggtaa gccaatgctc aattatttgc ttgggtcagg cttgggggaa attacagcgg
     4621 tggtggttcg ctactacggt ggtatccagc ttggcaccgg cggcttggtg aaagcctatg
     4681 gcaatggggt gcaacaggcg ttattgcagt tggagcggat tcgcaaagtt ttgcgaaaaa
     4741 actaccgctt gcaatgtgaa tatgagcagt ttaataccat ttctcatctc attagcgaga
     4801 aagatattgt gattgtcgag caacttttta gcgataaagt ggatttgttg ctggcattta
     4861 acccgagcga ggttgaggcc tttgcagatg aattaaccca acgcttttcg ggcaagctcg
     4921 cattaataaa agaagaaatc tagtctatgc aatttttttc cattattcgt attgttggca
     4981 ttttggtgat gtgtttttca ttcacaatgc ttgtgccggc aacggtcgcc ctaatttatg
     5041 gcgatggcgg tgggcgggcg tttttagaag ccttcgctct gaattttatg gtcggtacgc
     5101 tgctttggtg gctgtgccgt gacaacaaaa acgagctacg ttcccgtgag ggctttttga
     5161 ttgtagtact gttttgggtg gtgctgggtt tgctcggggc tgtgcctttt attattttgg
     5221 aaaaccccga tttaaacgtg gcggaatcca tttttgaatc cttttccggc ttaactacaa
     5281 cgggtgcaac cgtcatcacc ggcttagata gcttgcccaa agcgatttta ttctaccgcc
     5341 aatttttgca atggctaggg ggaatgggga tcattgtgtt agcggttgcg attattccgc
     5401 tgttaggcat tggtggaatg caactttatc gtgcggaaat gcccggaccg ctcaaagagc
     5461 agaaaatgcg acctcgcatt gcggaaacgg caaaagcgtt gtggctgatt tatctcagcc
     5521 tgacgattct gtgtgcggtc gcattttggc tcgccggaat gacggtgttt gatgccattt
     5581 cgcatagctt ttccacggtt tcgattggcg gattttctac ccacgatgcc agcatcggct
     5641 attttaataa tgcggcaatt agctatatta ccgtgttttt cctgctactt tctgcctgca
     5701 actttgcatt gcattttgcg gtgattgatc gcatacgaga tgcacaatca cgcaaaaaag
     5761 ggggtatttt aagccgcttg tattggggcg attacgagtt tcgttttttc ttgatggtgc
     5821 agttagggct tttcttggtt tgtaccttgg tgttagtggg ttacggttat ttcgataata
     5881 cctctgttac gattgagcaa gcgttgtttc aatcggtgtc catttccacc acggccggct
     5941 ttactaccaa tgattttagc caatggagtt cgtttttacc cacgctgttg gtgctgtctt
     6001 catttatcgg tggttgtgcc ggctcaaccg gtggcggctt gaaagtgatt cgggtgttgc
     6061 tgctttattt acaaagtaaa cgtgaaatcc accgctacat tcaccccaat gtgattcaac
     6121 cgattaaatt aggcaaccac gttttatcgg aacgggtggt tgatggtatt tgggcgtttt
     6181 tctcggcgta ctttttcgtg ttcgtgatct gctggcttgc taccatcgct tgtggaatgg
     6241 aaacctttga tgctctaaat gcggtaattg cctcactaaa taatctaggt cctgcattag
     6301 ggagcgtgag cagcaatttc acccaagtgc cggacagtgc caagttagtg ctgacttttg
     6361 caatggtatg cggacgtctg gaagtgttta ccttgctggt gattttaagc cctgtttttt
     6421 ggaaagatta aatgaaaacc ttaattctct attttacgac cgatggtcaa accaaaaaaa
     6481 ttgccgaacg attggcggac gtgattactc acgaagtcga gctgatttcg ctaaaagatc
     6541 aagcggtcga ttttgccgaa aaaattgcaa atgccgacca aattgtcatc ggtgcttcaa
     6601 ttcgctatgg gcattttaac ccgttggttt atcagtttgt ggaacagcat tatccggcat
     6661 taaacgccaa gaaaactgcc ttttacggtg tgaatttgac cgctcgcaaa gataatcgaa
     6721 acacgcctga aaccaatact tatgtgcgta aatttttagc taaagtgaaa tggaaaccaa
     6781 cccacattga ggtaattgct ggggcattac tttacctacg ctataagcca ttcgaccgat
     6841 tgatgattcg ctttattatg aaaattacca aaggcgaaac cgatacctcc aaagaatatg
     6901 agtacaccga ttggcagcag gtggagaatt ttggcaaatt attagccaat cagttataat
     6961 caaactgatc taaatcaaaa aaatgtaaat ttatttaaat gttgtagaaa ttttaaagat
     7021 tttgattata atcgccagcc cacgttcttg cgaagaccgt gtttaatcat tgaatgaaag
     7081 gaaagccccc ttgagtattt taaaagagtt ccgtgaattt gcggtaaaag gtaatgttgt
     7141 tgatatggct gtcggtgtga ttatcggcgg tgcattcggt aaaattgttt cttcattagt
     7201 tagcgatgtt gtaatgccac ctatcggttg gttaatcggt ggcgtggatt tcaaagattt
     7261 agccattgaa atcgccccgg caaaagaagg tgcggaagca gtgatgttaa aatacggtgc
     7321 atttatccaa aacgtatttg atttcttaat tatcgcactt gcggtattcg gtatggtgaa
     7381 agtgattaac tcactgaaaa aagctccagt tgtagaagaa gctcctgcag agccaacagc
     7441 ggaagagaaa ttattaactg aaatccgtga tttattgaaa aaataattaa ctgattttga
     7501 aacaagggct attttagaat atctagaata gccctttgtg ttatggttaa aaagaaaaaa
     7561 accaacataa agtcggtttt tttcatatct gttcgatatt acgctaattt tttgatttgc
     7621 gctgctaatt tagatttgtg gttagccgct ttgttagcgt ggattaagtt tttagatgcc
     7681 atacggtcta caactttttg catttcaacg aaagccgttt gagctgctgc tttatcgcct
     7741 gctgctactg ctgcatatac ttttttgatg aatgtacgca tcattgagcg ttggcttgcg
     7801 ttgtgttggc ggcgtttttc agattgaacc gcacgttttt ttgctgactt gatattagcc
     7861 aaggtcaaac tcctaaaata tctgtgtaaa aattagatat aaacaaaatc gttgtttata
     7921 ttgcctatcg atagttttta attcattttc aacgagacac gtgcttgaaa ataacaccgt
     7981 ttttctgata gaaaacgata gggcgagatt ttaacagttt ttttctgatt ttcctataac
     8041 aaactgctaa aattatgatc gatttttagg atcagactac aagcggtctg atttttcaat
     8101 aattttacta atattactct ttattatgag caaaaaactt ttaagatcag gaatgatcgt
     8161 aagctttatg accttaattt ctcgcatact cggtttgatt cgagacattg tggtggcaac
     8221 cattttaggc acaggcgtga gtgccgatat tttcctgttt gccaaccgta ttcccaattt
     8281 tcttcgccgt ttgtttgccg aaggggcatt ttctaaagcc tttgtgccgg tgttggcgga
     8341 atataatgcg gataacgatc caaacaaaac gagagaattt attgccaaag tatcgggtac
     8401 attaggaggt ttggtaactg ttgttaccct tgtggcaatg attgcctcgc ctgtggttgc
     8461 cgctcttttt ggtacaggtt ggtttttaga ttggctttac gacgggccaa atgccgaaaa
     8521 attcacccaa gcctcttttt tgctcaaaat cactttccct tatttgtggt ttattacctt
     8581 tgtggcgtta tccggtgcgg tgttaaatac catcggtaag tttggggtga tggcattttc
     8641 cccggtgttg ctgaatgtgg cgattatcgc ggtagcgtta tggggcagag attatttcga
     8701 ttccccggat attgccttag cgtggggggt atttttaggc ggcttattgc aatttttatt
     8761 ccaaattcca tttatgaaaa aagagggctt actggttaag ccaaaatggg cgtggaaaga
     8821 cgaaggtgtc actaaagtcc gtaaattaat gattccggct ttatttggcg tttccgttac
     8881 ccagctaaat ttactgctaa accaagtgat tgcctcgttt ttgattaccg gctcgattag
     8941 ttggatgtat tatgccgacc gcttaattga atttccgctc ggtttatttg gaattgcgat
     9001 ttccaccgtc gtgctgccaa gcctttcccg tattgcgaaa aacaaagaac tcactgaaat
     9061 ccaacgtgga gaacactttc aaaacacaat ggattggggg gtgagaatgg tgctgttgct
     9121 cggcattccg gcaatgatcg gtatggcagt gctggctcag ccgattatta tgacaatgtt
     9181 tatgcggggt aaatttggtt ttgaagatgt gctggcaacc tcttatccac tttgggtaat
     9241 gtgcttgggg ttaaacagct atatgttgat tagcgtgctg gcaaacggtt tttatgccaa
     9301 ccaaaacacc aaaacaccgg taaaagttgg gattattgcc gccttaagta atatttgctt
     9361 tggcttggcg tttgctccgt ttttgggata cattggttta gcgttagctt ctgcgtgttc
     9421 ggcattagtg aatgtgagct tgctgtatgt gaatctctca aaaaatggtt actataaagt
     9481 aagccataaa accgtgcttt ttgtgttgaa actattcatc gccgcttgtg taatgggggc
     9541 gttggttgcc tatttcacgc ctgagattga actttgggca ttaatgacaa tgtggcagaa
     9601 agtgtattgg ctgatttggc tgatgatctt agccggcggt agttattttg cctctatttt
     9661 ggtattaggc attcgtaaaa aagatttccg ctcggcataa aatgaaatca ttaaaactga
     9721 aaatcccgcc gccggcttgg ttcttgcttt gtgccgcttt aatgtggctg gtagctcaat
     9781 atgtgccggt gagcttgccg aattatcagc atacagccat attttttagc ggtttagctg
     9841 tggcgataat tggcggagcg gttgccgtct cggcactttg ggtcatacac caagcgaaaa
     9901 cgacatacag tccttttcga ccacaacata ccgctaaatt ggtcacttgg ggcatttacc
     9961 gcaaaagccg aaacccaatg tatctttcgt tactattagg tttgattgcg tgggcattat
    10021 ggttgggggc aatgttggcg tggctggttt taccgctgtt tgtatggttg ataacctatt
    10081 tccaaatcaa gccggaagag caaattttag cccaaaaatt cggcacacat tatgccaaat
    10141 attgcaacca agtaaggcgt tggctttaaa tgcaagcggt gagattttgt tggaaatttg
    10201 caaatttttc cgaaaatctc accgcttgtg gggttagtga ttgaaatcag ccgtcattaa
    10261 gtcgtgcgag ttgtccatcg ctaaattgat aaagcgttca tactgttgca gcacgtcggc
    10321 aatcaactct tctttggtca tatattgcac atcataacct ttccgcccgt ctaagaaata
    10381 agtaatcggc tcgtaaatgg tattttgctc aatgttcggt aggttgtcgt cacctaccac
    10441 caaatccgat aattcccgtg gcacactttc aatgccgtaa atgaaattgc gtaaattttc
    10501 tttcaccact tcaaactcta atttcgggtt ttgttcgttg gtgaaattca ttttagcgat
    10561 taagccatag ctttcaaatt cctcgataag ttctaaaaat gctggtctgg caactttaat
    10621 aaagaagtga cggacatctt gcttattgct tgggttgatg attttctcca agcgttgttt
    10681 ccaatcttta cccgcccaat gttggctacc ttgcgagaat tttttgttga agtattggtt
    10741 atctaccatt aatcctttcc ataagccgat acagagaatc agcataatga aggtaaatgg
    10801 taaggcgata atcagtgtca ttgtttgcag agaagccaag ccacccgaat agagtagtga
    10861 taacgctaag atggctaata atgctcccca aagcacgctt tgccatttcg gtgcattttc
    10921 ttcgccttgt gaagcaatgc tgttgagaac aaaaatcccc gagtcggcag aggtgataaa
    10981 gaaaatactg ataacaatga cggcaacgaa agaggcaata gagccaaacg gtaattgctc
    11041 aaaaaaggta aataacagtt gttcggtatt gccgctgact tcagctaaag caccggcagt
    11101 ttgttgatcg aaccaaatgg cagagctgcc gaagctggtc atccataaaa tgttaaacag
    11161 cgatggaaca aatagcaccc ctaaaataaa ttcacgaatg gttctgcctt tagagatctt
    11221 ggcaataaac aaaccaacaa atggcgccca agatgcccac catgcccaat aaaggaccgt
    11281 ccagccgctt agccagcctt gatgttccgg ttcataagcg aaggtacgga aactcatctc
    11341 aagcagcgag ctaaaataat agcctaaatt ttcggtgaag cttgagaaaa gtaatagagt
    11401 tgggccggtg attatcacaa aaataagtag caatccggct aatgtgagat tgatttcact
    11461 taagatttta acccctttgc ctacgcccga aacggcagat aacacggcaa gagccattgc
    11521 tactgttacg ataatcgcaa gaacagtaaa attgctctgt tcaattaaac ctagattgtt
    11581 aaagccagca ctgacctgca tcaccccaaa gccaagtgta gtcgttaaac cgaaaatcgt
    11641 gctacaaagg gcaataatat caattaaatg cccccagaag cctgaaatac gatgttttag
    11701 taagggataa aatccggaac gaatagtgag tgctaactta tatctgaagc caaaataagc
    11761 aagagctaag gcaatcacac cataaattgc ccaagcgtga ataccccaat gatagaacgt
    11821 tgtcatcatc gcttctttca tacgctccgg ctcagttaaa ttttgttgga cgggtttaag
    11881 gtaatgcaaa ataggttctg ctaccccaaa atacattaac ccgatcccca ttccggcggc
    11941 gaataacatc gcaatccaag agccaaaacc aaattcaggc tcatcggtat ctgcccctaa
    12001 tttaatatca cccaatcggc tgaaagagag aaaaattaga aacaggaaaa agatagagcc
    12061 tgcaaaaata tagaaccagc taaaatgttg gaagagacta tttttgatgg tatcaagtga
    12121 ggtttgtgct tgctgtggaa agatgataca gaaaacggca acaaagctga caaagagtaa
    12181 actcggtagg aaaatcggta ccctaaaggt acttcctgtg tttaattttt tgattaaatc
    12241 caatgctatt tcctctattt ttgaatggtt agaggcttga atatagcaaa aagatataaa
    12301 aataacaaat ttaagacgta ttggtaataa tattgcaacc taatgggttt ggtaaaaaaa
    12361 tcgggaaatt tgaccgcttg cacattcatt atttgcgagt agaatatttt tcatttataa
    12421 aaaacgaata aagaatgcac acacaacata acgctcaaaa aatggttttg cccattttac
    12481 tgctgatttc cctttctcat ttgttgaatg atttagttca agcagtaatg gtttcgatct
    12541 atccaatgct aaaagaaaat taccaactga gttttgctca aattggtatg atttcactca
    12601 tttatcaaat taccgcatct attattcagc cgggtattgg gctttatacc gatacccaaa
    12661 accgaattta ttgcccgtca gtatgctgat tacgcttctt tccgtgattt tacttgcctt
    12721 ttcgccgaat tttggcgtgt tactgatagc cgctgcgtta atggggatag gctctgccat
    12781 ttttcaccct gaagcctctc gggtggctcg aatggcatcg ggcggtaaat ttggtacagc
    12841 tcagtcgctt ttccaagtag gtggaaacag tggatcggcg ttaggtccgc tcttagttgc
    12901 attatttatt gtgccgttag ggcaaagtgc ggtgatttgg gttgttgcat ttgtattatt
    12961 ggcattatga tgggttttat ataaagtaag ccgttgggtg aaatatgaag ccaaaccgct
    13021 cattatttca ccaaaccata ttcaacaacg cttacacggt aaattattag ccagagcatt
    13081 attaattatc gctttattga tgttagcgaa atttatttat attgcgagcc tcagtaacta
    13141 tttcaccttt tatttaatgg aaaaattcca actcaatata gcaatggcac agctctctct
    13201 atttaccttt ttaggtgcag ttgctttagg cgcttttgtc ggcgggccga ttggcgataa
    13261 gattggacga aatgcggtga tttggttctc tttcttaggt atggcacctt ttgctttgtt
    13321 gttgcctcac gcagatctct tttggacgat tgtgtgtgcg atttttgccg gttttattat
    13381 gtcatcagcg ttctccgcta tggtggttta tgctcaagaa gccgtaccag gcagagtagg
    13441 gctgatttcc ggcacaatgt tcggtttaat gtttggtatt ggcggcttaa gtgccgccgt
    13501 attgggtttt tttgcggatg aatatggctt ggagagtatt tttaaagcct gtgcctattt
    13561 gccactactt ggttttgctg cattagggct accaaaacga aatatttaac gttttatttt
    13621 tagcgtttga caccagcttt tttcataccc aataagaaaa cccccacccc aatccaaacc
    13681 agaatgtagc ctacagcacg ttcggcggaa agtgtctcat taaaaatcag cacaccgcat
    13741 aaaaattgca gggtaggtga aatatattgc agcataccga gtagcgacat tgaaatgcgt
    13801 cttgcaccca ttgcaaacca tagcaatggt aaggtagtgg caatgccgga gcagagtagc
    13861 actgccattt gtaaaggatt gagttcactg aataccaaaa tgttttggtg gtaacaaaag
    13921 cagagatagg tgatggcaaa aggcgacatc agcaaagttt ctaacgccaa accagacaaa
    13981 ggttccattg gggcaagttt acgaatcagc ccgtaaatac agaaactggc ggctagaatc
    14041 aatgcgaccc acggaatttg tccggcagga atagcaagcc ataaaatgcc gctaattgca
    14101 aacagcagag ccaataattg ggctttattc agctgctctt ttagaataag cctaccgaca
    14161 aacacgttga aaatggggtt gataaaataa cctagactgg catctaaaat atgctcattg
    14221 acgattgccc ataagtaaac cagccagttc acgccaatca taaaagaaga aagcacgaac
    14281 agcccgagta cctttggctg tttaaaggca ttaataaccg ctcgcccttg ttggaaaatc
    14341 agcagcagaa acacagcaaa tagggcagac caaagcaccc gttgagccaa aatttgctcg
    14401 gcaggcattc ccgattggtt gatcggatac caataaatcg gaaaggttcc ccaagttaag
    14461 tagcacagta gggcatagtg ccaaccctgt aatttgcctg aaatattcat tttgctttcc
    14521 taaacgagtg tacaagcggt catatttgtc gatttttttg caaattgcaa atcacaaaac
    14581 ggcataaaaa attaggcttt ttcaatgccg aaagaaataa cctcttcaat tttttccgca
    14641 tccattgcca gcattaacag cctatcaacc ccaagggcga cacctgagca gtttggaaaa
    14701 cccgctttga gtgcggcaag aaaacgcaca tctagttgct gtggctccag ccccattttt
    14761 tcacgttgca gattatcttg ctcgaagcga tgaatttgct catcagcatc gtctaattcg
    14821 tggaagccgt tacagagttc caagcctttg tagtaaaact caaaacgttc ggcaacacgg
    14881 tgatcttccg agctaatttg agctaatgcc gcttgggtgg aagggaaatg ataaacggca
    14941 gttggtcttt cttgcccgat attggcttcg acaacctcgc tgaataaaaa ttgtaacaag
    15001 gtgtcacgct gttcatcatc atcacattgc aagccgtgtt ttcgggcttt ttccacaagt
    15061 tgtgctttgg tggcagaaag cggatctaat ccgacatagg tttggaacac aaattgatag
    15121 ctgaatgatt ctgccggttc gcagtccaaa atttgttgca gtaaatcatc cacttcattg
    15181 attaagcgat acatatcaaa atgcgggcga taccactcca gcatcgtaaa ttcagggttg
    15241 tgtcgcttac cggcttcctc gttgcgaaat actttacaca gttggaaaat cggtccactc
    15301 ccagcagcaa gtagtcgctt catatgatat tccgggctgg tcatcagatg gagtgttttc
    15361 gcctcagggg aaaaaggcga ttgaaactgc gtattgaaag tcgaaagatg aacgtctgtc
    15421 accgaaaatt cgcttaaaat cggggtttct acctctaaaa gccctcgatc ggtaaagaac
    15481 ttgcggattt ccgtaatgat ttttgaacgt tggatcaggt gaggaataga ggctgttggt
    15541 tgccaatcaa tattatcgag ttgtaaattg ttcataaaaa atgaccgctt gaaaatgtga
    15601 ggctatttta gcctaaaaaa gtgagttgcc ctagttaccg atcaagaaaa ataaagagag
    15661 ggttttaact tttttgcaac aaattaaatt ttctcatttt aaacattaga aattttcttt
    15721 ctatca
//
LOCUS       DANLXS010000041        15468 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.041, whole genome shotgun sequence.
ACCESSION   DANLXS010000041 DANLXS010000000
VERSION     DANLXS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15468
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.041"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(29..757)
                     /gene="cmoA"
                     /locus_tag="PXD71_000953"
     CDS             complement(29..757)
                     /gene="cmoA"
                     /locus_tag="PXD71_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDL6291096.1"
                     /translation="MQRDTLFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITA
                     IGMLAERFVTDNSNVYDLGCSRGAGILSIRRNVNAKNVQIIGVDNSQPMVERCKTHLS
                     AYHSEIPVTILLDDIRHIEIKNASMVVLNFTLQFLPREDRLALLQKIYQGLNSNGILV
                     LSEKFTFESEEMNELLIDLHHTFKRANGYSELEVSQKRNALENVMRTDSIETHKKRLQ
                     EAGFTQVELWFQCFNFGSMIAIKA"
     gene            complement(767..1549)
                     /locus_tag="PXD71_000954"
     CDS             complement(767..1549)
                     /locus_tag="PXD71_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL6291097.1"
                     /translation="MTEKSQLNFNYQPSNNHANAQTMIFLHGLFGDLNNLGAIAKPFA
                     ERYSILMVDLRNHGRSFHSDEMNYALIAQDLAQLLTELNIQNAIVVGHSMGGKSAMAL
                     AAFAPELVEKLVVIDIAPVTYTQNRHQQVMAALFAVKEAKPQNRQQAKAIMAEYIRDE
                     GVQQFMLKSFDAQSEQSFRFNLQALKDNYQNVMGWQQVFVDKPTLFIKGALSDYIQAE
                     HTQTILAQFPQAKSFIVANADHWVHAEKTGTVVRAINKFLEK"
     gene            1668..2234
                     /gene="seqA"
                     /locus_tag="PXD71_000955"
     CDS             1668..2234
                     /gene="seqA"
                     /locus_tag="PXD71_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDL6291098.1"
                     /translation="MKRIEIDDELYQYIASRTQSIGETASDILRRLLRLPQSPQPFVL
                     VQEHMINELKELVKTPSRATKKNESNAEKTVEKLENILNSEHFMNENKNVVRFTMLLA
                     ALYRANPEAFAKATEKVRGNERIYFSQSEAEILATGSGVKAKQIPDSPFWVITNNNTA
                     RKGLILKGVMESMKVPSQLIERIQALFI"
     gene            2244..3662
                     /gene="menE"
                     /locus_tag="PXD71_000956"
     CDS             2244..3662
                     /gene="menE"
                     /locus_tag="PXD71_000956"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDL6291099.1"
                     /translation="MLINSTRFPTQFWAAHSAQNVAIIWEKGTSDYLNHLPSNITWYS
                     LNQLIAQAIFVLQQQGATPHSLIAYCGSHRLIGLLTYCASLALGAKILMLNPAQTASQ
                     RQAVLDDNGVELIINDADFAKFSAKTTACNAFPTIDFEKPATLTLTSGSSGKPKAVVH
                     SIAAHLYSAEGVCELMAFEQPNSWLLSLPLFHVSGQGIIWRWLFKGATLYVHEDKSDF
                     FLSAQKVSHLSLVPTQLQRYLNQLDSMVSQKCLLGGSMLPAELIAQAQQSGMTTFSGY
                     GMTEMASTICAVENDLNSVGFPLKHRELKLENGEIWVKGKSLGLGYWQKNGQIRPLVN
                     TQGWFATKDRGEWNHKKQLVIKGRLDNMFISGGENIQPEEIEQLIFRSQLVEQIIVLP
                     IEDKQFGHRPVAFLQFKQSDSKNELHKLKIWLSDKLERFKQPVAYYLLDIEKYQKQGL
                     IKLSRTQLQQDLKLKVLKEIYV"
     gene            3655..7032
                     /gene="mscK"
                     /locus_tag="PXD71_000957"
     CDS             3655..7032
                     /gene="mscK"
                     /locus_tag="PXD71_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL6291100.1"
                     /translation="MFRPILKTFVLSLGLMLNSYALAELPTQQVVQTQLDSAKKLDQA
                     DADNKALVQTLEDTLAFLAQIDKQKADNQALNKSIQDSQKALKTSQHNLEKLKEVTIA
                     SVDNLTQKATTDLQKELTSTQSQLETVQHDLSIINNNLVAQNTAPDKAQTALTEMVKR
                     KQEISSQLSATNIKNELQTKLEVELELIELKNTYNQILLGGSEDLSALYSAQLDEKKQ
                     EQQNLQAEIANLQTAINNKIVEESQNKLDQAQAVQNQQNNATTNPVILRELDINTKVT
                     QELLKQTKDMTQLSQDNLRIKSVLDNLQQTQRNIEEQISSLQGTLVLSRIINKQKQSL
                     PQDEMISGLSKQIADLRVRVFDITEFKDSFADINAYISRIEQDEKTTFTSKEKEQLSK
                     ILQERSDTLTEMIKSLNNQLNLLINIELNQQQVQTISDALQQKLQQQSFWVKSNNPID
                     LEWFSKFVDITHYQFIDIGKKLNFSNWRDNLLPASILVGILLLLAIFINRQKDKIKQK
                     LHQINNRLKIVATDSQWNTPLAIFWTLVLCLPSTLMFLAGFVAITYISFENPQEVWTW
                     GLKMAIYWLYFAFLVAMLRPHGIGHIHFNMPQKSNEIFRNILKRSVWIIALLLNTSIF
                     TNLEMGISYDMLGQTMTIFVLILMIWMIVPAFRQAISTYENVTTEEKKESKSILLTIV
                     KLVLLLAPIILIILIALGYYYTALVIIEHLVDSYFAIITWIIIRNILYRAFSISSRRL
                     AYRRLQEKREAAEAKTRAKAEQNDENPDGVELPFELKEDSIAVSEVKNQMLKLTDFVL
                     WIGLFVLLYWVWSDLITVALYLDGVTLWQQSVTTEAGIVMESITLLNLLTAILIIVVM
                     VVLIRNLSGLLEALIFSHFKLSQGTPYTITTLLTYLIIALGAIFAFGTLGMSWSKLQW
                     LLTALSVGLGFGMQEIFANFVSGIIILFERPMRIGDTITIGTYSGTVSKIRIRATTLV
                     DFDGKEVIVPNKAFVTERLVNWALSSTATRIIVQVGVAYGSDLELVKRLLLQVAEENP
                     KVLKDPQPRAYFLTFGASTLDHELRAYVNDLSDRNPTIDALNTRIHQLFNEHNIDIAF
                     NQLDVYIKNNDTQDEIKVDSKRFEFERPNNITAKEKE"
     gene            7035..8117
                     /gene="aroC"
                     /locus_tag="PXD71_000958"
     CDS             7035..8117
                     /gene="aroC"
                     /locus_tag="PXD71_000958"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL6291101.1"
                     /translation="MAGNSIGQLFKVTTFGESHGIALGCIVDGVPPNMALSEADLQPD
                     LDRRKPGTSRYTTPRREDDEVQILSGVFEGKTTGTSIGLIIKNGDQRSQDYGDIAEKF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETAMRVAAGAIAKKYLREQFGIEVRGYLSQI
                     GNIKIDPKTVENIEQIDWHAVNSNPFFCPDPVAIEGFDALIRELKKEGDSVGAKLTVI
                     AERVPVGLGEPVFDRLDADLAHALMSINAVKAVEIGDGFAVVEQRGSQHRDEMTPQGF
                     LSNHAGGILGGISSGQPIIAHIALKPTSSITIPGRSVNKNNEAVEVITKGRHDPCVGI
                     RAVPIAEAMMAIVLLDHLLRFKAQCR"
     gene            8136..8963
                     /gene="mepA"
                     /locus_tag="PXD71_000959"
     CDS             8136..8963
                     /gene="mepA"
                     /locus_tag="PXD71_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDL6291102.1"
                     /translation="MKLLKTLLVSSLAFTATALNATQWEKIKTPVQGKAQSIGGYSNG
                     CIIGAQPLALKGEGYQVIRSARNRYYGHPQLLDYLTHLGKKTKAAGVPTILIGDMGMP
                     AGGRFASGHASHQTGLDADIWFKFGPMDDETARNPAGLATIMVNRSTERVDDSLWNQH
                     HTNLVKLAATDPRVDRIFVNPAIKVKLCQTAGNDRAWLRKIRPWYGHDSHFHVRLTCP
                     ADAASCENQALVPAGDGCGEELYSWFEPKEASGTPKAKVLPTAPVQCQMILSEQGLN"
     gene            9036..10955
                     /locus_tag="PXD71_000960"
     CDS             9036..10955
                     /locus_tag="PXD71_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL6291103.1"
                     /translation="MRLFVAEKPSLARAIADVLPKPHTKGDGFIQCGEHDTVTWCIGH
                     LLEQAEPDVYDERFKMWRMEHLPIVPEQWKLIPKKETLKQFKVVEKLIKKADVLVNAG
                     DPDREGQLLVDEVFGYLNLSAERKSQIQRCLVSDLNPNAVSRAIEKLQKNSDFIPLAT
                     SALARARADWLYGINMTRAYTLQGRWAGYKGVLSVGRVQTPVLGLIVRRDLEIEHFVP
                     KDYFEVLAQIVVPETQERFKAQWQPSKACEDYQDEDGRVLSRPLAENVVKRITDQPAT
                     VTEYQNKIEKETAPLPYSLSVLQMEAAGRYGLSAQAVLDICQKLYETHKLITYPRSDN
                     RYLPTEHFNDRFKVMAAIAHHLPEYQQKPEVIDATIRNRCWNDKEVEAHHAIIPTARQ
                     GTVHLTENEKRVYQLIARQYLMQFCPDAEYRKSKIGLEIAGGSFVAQARNLVIAGWRA
                     LLGKEDSLDDAEPLLPIVKKGQILHCEKGEIVSKKTQPPRHFTDRSLLSAMTGIARFV
                     QDKELKKILRETDGLGTEATRAGIIELLFKRGFLIRKGKNIHSTEAGRILIQALPDSA
                     TQPDMTAHWEMQLNQISKKETSYQQFMFDLNQQLPHLLQSPNRQVLQNLAKITPLASG
                     NKPRYFKKRTQTAEN"
     gene            11061..11387
                     /gene="secG"
                     /locus_tag="PXD71_000961"
     CDS             11061..11387
                     /gene="secG"
                     /locus_tag="PXD71_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL6291104.1"
                     /translation="MLINVLTIVYLVVAVLLIAFVLMQQGKGADAGASFGAGASATVF
                     GSAGSANFLSRTTALLATIFFAISLVIGNLSSHNVAPKSQFEDLSQVEQKQVEPVKIE
                     NSDIPQ"
     gene            11412..11497
                     /locus_tag="PXD71_000962"
     tRNA            11412..11497
                     /locus_tag="PXD71_000962"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11446..11448,aa:Leu,seq:gag)
     gene            11700..12941
                     /locus_tag="PXD71_000963"
     CDS             11700..12941
                     /locus_tag="PXD71_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6291105.1"
                     /translation="MARTTKALNKTQIENAKSKDKEYILSDGDGLNLRVKPLPKGSKI
                     WIFNYTNPQTKNRTNLTIGRYPLITLAEARTICDGYRSLIEKGIDPQEEKKRLQQEAE
                     NRVNDTFRNVAESYFNGIYKEKAKNPITREKNWIRLENHIFPYIGDKHVEEIKVKALV
                     DIYSKVADKSNTLKKLHQLVSAVMDHAITQGIIESHNCRLAVKNFYIKPSTPNPTIEL
                     EELPQLFTDLHNAKLSKQTYALVCWSFLTALRPNEAVNAEWKEIDFEKKLWNIPKEKM
                     KGKADKKRPHSVPLSSQALKILEMMKMHSNSSPFVFPGRSSNSKPMSNATVNSTLKRN
                     GYKDRLTAHGIRAFVKTFLSSRKVDRHVSESILSHLLEGGDDLENTYNRYDFLEERVP
                     VMQLLGDYCEQCGMNLELVRG"
     gene            complement(13100..14968)
                     /locus_tag="PXD71_000964"
     CDS             complement(13100..14968)
                     /locus_tag="PXD71_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL6291106.1"
                     /translation="MFKKLLELRQQKAAIEAEMRSILTKADEEKRSTTEAEGAKFDEL
                     RSQAENLVKEIARYEAITDEQRNQPGQQVETANVKVSDDELRHWIKTGELRSLATNTN
                     GGADGGYSVIPELDKQVMKRLTDDSVMRQIANVVKLTQGKEYKKLVSAGGAAVNHIGE
                     GEARTETATPKMNEVTIAVHNIYAYPKTTQEILDFGGVDILGWLTEEISESFVETEET
                     DLTNGTGTKQAKGFLAYPRTTANDKTRPFGTLQKMEVQPATVIGWNSKGQPETLTPYP
                     PYSVNVQRTVNRQNWLTAYIPHSYDENVAETPHFDQWLNFVSDGKQDKAKNILAALYA
                     ILTNRYNWQVFFQVTGKGGSGKSVFAGIATLLAGEKNTASARLENFDDERKIAGLEDK
                     KLIICSEQTKYAGDGGGLKAITGGDMVRVDPKNKHPFNARIAAMIMIVNNDPCKWTER
                     NGGIDRRIVNFRFNKRPPENERDPYFMDKITLEIGGIIRKVLDTFPDPLEAKRALEEQ
                     KESLEALEIKKQSDPLTDFFEYLYTTENTDGLYIGTGNTLGHDKIRTHLYPAYLAFVK
                     AKNVLELGLNTFVVGIEQAVKQHGNKHDFTKRKTNKGQRTNVHFKNFDDFQSDILN"
     gene            complement(14979..15374)
                     /locus_tag="PXD71_000965"
     CDS             complement(14979..15374)
                     /locus_tag="PXD71_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6291107.1"
                     /translation="MISNEAIRQEVIDLLQGNIQGIQHFHNGRAVFTDVENELPAIAV
                     FIDEAEYSQLTLCDGECNAYLKIGIYLPMFSTENDLDLVAQKISDVMNQATFHSMDEC
                     VLAKYSYDYDANESAWKTATLHYNINYLN"
BASE COUNT         4776 a         3203 c         3073 g         4416 t
ORIGIN      
        1 caaaaaattt tacaaatatc accgcttgtt aggctttaat tgcaatcatt gagccaaaat
       61 taaagcattg aaaccaaagt tcaacttgtg taaaaccagc ttcttgcaga cgttttttat
      121 gggtttcaat actatctgtt cgcatcacat tttccaaagc attacgcttt tggctgactt
      181 ccaattcact gtagccattt gctcgtttga atgtgtgatg aaggtcgatc aatagctcgt
      241 tcatttcctc actctcaaag gtaaattttt ccgataacac taaaatgcca ttggaattta
      301 atccttgata aatcttctgt aataatgcta ggcgatcttc tcttggtaaa aattgcaagg
      361 tgaaattaag taccaccatt gaagcatttt taatttcaat gtggcgaata tcatcaagta
      421 aaatcgtcac cgggatctca gagtgataag cacttaaatg ggttttacaa cgctcaacca
      481 tcggctgaga attatctacc ccgataatct gcacattctt cgcattcaca ttacggcgaa
      541 tagataaaat ccctgcgcct cgtgaacaac ctaaatcata aacatttgaa ttatctgtga
      601 caaaacgctc cgccaacatt ccaatagcag taataatatt ggaatagccc ggcacggagc
      661 gttggatcat atccggaaac acttcagcaa ccgattcatc aaaagtaaaa tcgcccaatt
      721 tctcaatcgg agcggaaaat aatgtatctc tttgcatttt tctcccttat ttttctaaaa
      781 atttattgat tgctctcaca acagttcctg ttttttctgc gtgaacccaa tgatcagcgt
      841 ttgccacaat gaaagatttg gcctgcggaa attgtgccaa aatcgtttgt gtatgctctg
      901 cttgaatata atccgataat gcacctttaa taaacaaggt cggtttatcc acaaatacct
      961 gctgccaccc catcacattc tgataattat cttttagcgc ctgtaaatta aaacggaaag
     1021 attgctcgct ttgggcatca aaagatttca acataaactg ttgcacgcct tcatctcgaa
     1081 tatattcagc cattatcgct ttagcttgct gacgattctg gggtttagct tctttcaccg
     1141 caaataaggc tgccataacc tgctgatgcc gattttgggt ataagtgacc ggggcaatat
     1201 caatcactac caatttctcc accagttcag gagcaaatgc agccaatgcc atcgcacttt
     1261 ttccgcccat tgaatgccca accacaatag cattttggat atttaattct gtgagtaatt
     1321 gtgctaaatc ttgtgcaatc aaagcataat tcatttcgtc tgaatgaaaa gatcgcccgt
     1381 gatttctgag atcaaccatt aaaatggagt aacgttctgc gaatggtttt gctattgcgc
     1441 ctaggttatt taaatctcca aacaatccat gtaagaagat catcgtctga gcattagcat
     1501 gattattcga aggttggtaa ttaaagttta actgcgattt ttctgtcata taaatgtcat
     1561 tgttcatata aattttcagc gatgttcctc tataaaaaac aatagaaatg cgtataatag
     1621 caagcactat taacgcattt aatacccgca gatatttgag gatcccgatg aaacgaatcg
     1681 aaattgatga tgaattatat cagtatattg caagccgtac acaatctatt ggtgaaactg
     1741 cgtcagatat attacgccgt ctattacgcc tgccacaatc gcctcagcct tttgttttag
     1801 tacaagaaca catgatcaac gagcttaaag agcttgtcaa aacgccttct cgtgctacga
     1861 aaaaaaatga atccaatgca gaaaaaaccg ttgaaaaatt ggaaaacatt ttaaattccg
     1921 agcattttat gaacgaaaat aaaaatgtag tgcgttttac tatgctatta gccgcattat
     1981 atcgagcaaa tccggaggct tttgctaaag caaccgaaaa agtgcgtggt aatgaacgta
     2041 tctacttttc gcaaagtgaa gccgaaattt tagctactgg gagtggagta aaagccaagc
     2101 agatcccaga ttcaccgttt tgggtaatta ccaataataa taccgcacgc aagggactta
     2161 ttctcaaagg cgtaatggaa tccatgaaag taccaagcca gttaatcgaa cgcattcaag
     2221 cgctttttat ctaattaatt gaaatgctca tcaattctac tcgctttcct acccaatttt
     2281 gggctgctca ttcagcccaa aatgtggcga ttatttggga aaaaggaaca tcagactatc
     2341 ttaaccacct tccatcaaac atcacttggt atagtttgaa tcagttgatt gcgcaagcta
     2401 ttttcgtgtt gcaacaacaa ggtgctactc cgcacagttt gattgcctat tgtggttctc
     2461 atcggctcat tggcttgctc acttattgtg ctagccttgc tttgggggct aagattctaa
     2521 tgcttaaccc tgctcaaaca gcaagtcaac gacaagcagt cttagatgac aatggtgttg
     2581 agctcattat taatgatgcc gactttgcaa aattttcagc aaaaacgacc gcttgtaatg
     2641 ccttccctac gattgatttt gaaaaaccag ctaccctaac cctaacctca ggctcaagcg
     2701 gtaaacctaa agcagtggtg cattctatag cggcccatct ctatagtgcc gaaggagtat
     2761 gtgaattaat ggcatttgag cagccaaact cttggctact gagcttgcct ctgtttcacg
     2821 tttccggaca aggcattatt tggcgttggt tatttaaagg tgcaacactg tatgttcacg
     2881 aagacaaaag tgattttttt ctttcagcac aaaaagtgag ccatctctct ttagtcccca
     2941 cccaattaca acgctattta aaccaattag attctatggt cagccaaaaa tgtttactgg
     3001 gtggatctat gcttccagcc gaattgattg ctcaagcaca acaatctggg atgactacct
     3061 tttcaggcta cggaatgact gagatggcat caaccatttg tgcagtagaa aatgatttaa
     3121 acagtgttgg ttttccgtta aaacatcggg aactaaaatt agaaaatggt gaaatttggg
     3181 taaaaggcaa atcactcggc ttaggctatt ggcaaaaaaa tgggcaaatt cgaccgcttg
     3241 taaacacaca aggctggttt gccactaaag atcgtggcga atggaatcac aaaaaacagc
     3301 ttgtaataaa aggacgattg gataatatgt ttatttcagg aggggaaaat atccaacctg
     3361 aggaaattga acaactgatt tttcgttctc agcttgtcga acagattatc gtgttgccga
     3421 ttgaagataa gcaatttgga catcgaccag tggcgttctt acaattcaag caatctgatt
     3481 caaaaaatga actccataaa ttaaaaattt ggctgtctga taaattagaa agattcaaac
     3541 agcctgttgc ctattattta ctggatatag aaaaatacca aaaacaaggc ttaattaaac
     3601 tatcaagaac acaattacaa caagatttaa aactcaaagt attaaaggaa atctatgttt
     3661 agaccgattt taaaaacatt tgtgcttagt ttaggcttaa tgctaaatag ttatgcatta
     3721 gctgaattgc caacccaaca ggtagtgcaa acgcagttag atagcgccaa aaaattagat
     3781 caagccgatg cggataataa ggcattggtt caaacacttg aagatacgct tgctttctta
     3841 gcccaaattg ataaacaaaa agcagataat caggcattga ataaatctat tcaagattcc
     3901 caaaaagcat taaaaacaag ccaacataat cttgaaaaat taaaagaggt gactattgca
     3961 agtgtcgata atttaacaca aaaagcgaca acagatttac aaaaagagtt aacctctaca
     4021 caatctcagc tggaaacagt acaacacgat ttaagtatta ttaataataa tttagttgca
     4081 caaaataccg caccggataa ggctcaaacg gccttaaccg agatggttaa acgtaaacag
     4141 gaaattagta gccaactttc agcgactaac attaaaaatg aactacagac aaagttagaa
     4201 gtagaattag aattaataga attaaaaaac acttacaatc aaatattact tggtggcagc
     4261 gaggatttat ctgcgcttta ctctgctcaa ttagatgaaa aaaagcaaga gcagcaaaat
     4321 ctacaagcag aaattgcaaa cctgcagact gctatcaaca ataaaattgt agaagagtct
     4381 cagaacaagc tagatcaagc acaagcggtt caaaatcagc aaaataatgc aacgacgaac
     4441 ccagttatct tgcgtgaatt ggatataaat accaaagtca cacaagagtt gctaaaacag
     4501 accaaagata tgacgcagtt atcacaagat aatttgcgaa tcaaaagtgt gctagataac
     4561 ttacagcaaa cccaacgtaa tattgaagaa caaatcagtt ctctgcaagg tacgttggtg
     4621 ctttctcgta ttattaataa gcaaaaacaa tcattaccac aagatgaaat gatttccgga
     4681 ctatcaaaac aaattgcaga tttacgtgta cgtgtttttg atatcactga atttaaagac
     4741 tcttttgcgg acataaatgc ttatatcagc cgcatagagc aagatgaaaa aacaaccttt
     4801 acgagtaaag agaaagaaca actgagtaaa attttacagg aacgttctga cactttaact
     4861 gagatgatta aatcattaaa taatcagctc aatttattaa tcaatattga actaaaccag
     4921 caacaagtgc agaccattag tgatgcgtta cagcaaaaac tgcaacagca aagcttctgg
     4981 gttaaaagta acaaccccat tgatcttgaa tggtttagta aatttgtcga tattacccac
     5041 tatcaattta ttgatattgg aaaaaaactc aatttttcaa attggcggga taatttatta
     5101 cctgcaagta ttttagtcgg aattttattg ctattagcca tctttattaa tcgccaaaaa
     5161 gataaaatta aacaaaaatt acaccaaatt aataatcgac taaaaattgt cgccacagat
     5221 agccaatgga acacccctct tgctattttt tggacattag tgctttgcct gccaagtaca
     5281 ctaatgttct tagccggatt tgttgctatc acttatattt catttgaaaa tcctcaggaa
     5341 gtctggactt ggggattaaa aatggcaatt tattggctgt attttgcctt tttagtggcg
     5401 atgctacgcc cgcacggcat aggacatatt cactttaata tgccacaaaa aagtaatgaa
     5461 atcttccgta atattttaaa acgttcggta tggatcattg ctttattact gaacacctct
     5521 atttttacaa acttagagat gggaatatct tacgacatgc ttggacaaac aatgactatt
     5581 tttgtcctta tattaatgat ttggatgatt gtaccggcat ttaggcaagc tatttctacc
     5641 tatgagaatg tcacaacaga agagaaaaaa gaatctaaaa gtattttact cactattgtc
     5701 aaattagtgc tactgcttgc ccctatcatt cttatcattc tgattgcatt gggctattac
     5761 tatactgcac tggtgatcat tgagcattta gtagattctt attttgcgat cattacttgg
     5821 attattattc gtaatattct ctatcgagca ttctctattt catcacgccg tctggcttac
     5881 agacgtttac aagaaaaaag agaagcagct gaagccaaaa cacgagctaa agcagaacaa
     5941 aatgatgaaa acccggacgg tgtcgagctt ccttttgagc taaaagaaga ctctattgcc
     6001 gtaagcgaag tcaagaacca aatgttgaaa ttaactgatt ttgtgctttg gattggctta
     6061 tttgtgctgc tttactgggt ttggtcggac ttaattactg ttgcactcta cctagacggc
     6121 gttaccttat ggcaacaaag tgttacaaca gaagccggga ttgtaatgga atccattacc
     6181 ctgctgaatt tactcactgc tattctgatt attgtggtaa tggtggtact cattcggaac
     6241 ttatccggtt tacttgaagc cctgattttc tcgcacttca agttatcgca aggtacacca
     6301 tacacaatta ccacactctt aacctatttg attattgcgt taggggcaat ctttgcgttc
     6361 ggtacattag ggatgtcttg gagcaagctc caatggctac taactgcact ttctgtcggt
     6421 ctgggttttg gtatgcaaga gatctttgca aacttcgttt cagggattat catcttgttt
     6481 gaacgtccaa tgcggattgg cgacaccatt accatcggca cttacagcgg tacggtttcc
     6541 aaaattcgta ttcgtgccac aaccttagtc gattttgacg gcaaagaagt gattgtacca
     6601 aacaaagcat tcgtaaccga acgcttggtg aactgggcat taagcagcac cgcaacccgc
     6661 attattgttc aagtcggcgt ggcttacggg tctgatttag aattagtcaa acggctgtta
     6721 ttacaagtgg cggaagaaaa tccgaaagtc ttgaaagatc cacaaccaag ggcttatttc
     6781 cttacctttg gtgcaagcac tttagatcac gaattaagag cctatgtgaa cgatttatct
     6841 gatcgtaacc caactattga tgcactaaat acacgcatcc accagctctt taatgaacat
     6901 aatattgata ttgcatttaa ccaattagat gtttatatca aaaacaacga tacacaagat
     6961 gaaattaaag tagattcaaa acgctttgag tttgaacgac caaataacat aacagcaaaa
     7021 gaaaaggaat aacaatggca ggcaacagca tcggacaatt atttaaagtg accactttcg
     7081 gcgaatctca cggtattgca ttaggctgta tcgtagatgg cgtaccgccg aatatggctt
     7141 taagcgaagc agatttacag ccggatttag accgccgtaa acccggtact tcacgctaca
     7201 ccacgcctcg ccgtgaagat gatgaagtgc aaattttatc gggagtcttt gaaggcaaaa
     7261 cgacgggaac aagcattggt ttaatcatta aaaacggtga ccaacgctca caagactatg
     7321 gcgacattgc cgaaaaattc cgcccggggc acgcagatta tacctatcag caaaaatacg
     7381 gcattcgaga ttaccgtggc ggtgggcgtt cttctgctcg ggaaaccgca atgcgggttg
     7441 ccgccggggc gattgccaaa aaatatttgc gtgagcaatt tggcattgaa gtgcgaggct
     7501 acctctcaca aatcggcaat atcaaaattg acccgaaaac cgttgaaaat attgaacaaa
     7561 ttgattggca cgcagtcaat agtaatcctt tcttctgccc cgatcccgtt gcgattgaag
     7621 gctttgatgc cttaattcgt gagctgaaaa aagagggcga ttcggtgggg gcaaaattaa
     7681 ccgtgattgc agagcgtgta cctgtagggt taggcgaacc tgtgtttgac cgcctagatg
     7741 ccgaccttgc ccacgcctta atgtcgatca acgccgtgaa agcggtggaa attggcgatg
     7801 gttttgcagt tgtggaacaa cgtggtagcc agcaccgaga tgagatgacg ccgcaaggct
     7861 ttctctctaa tcacgccggc ggaattttag gtggtattag ctccggtcag ccgattatcg
     7921 ctcatattgc gttaaagccg acctcaagca ttactattcc cggcagaagc gtgaacaaaa
     7981 acaacgaagc cgttgaagtg atcactaaag gcagacacga cccctgtgta ggtattcgtg
     8041 ctgtgccgat tgcagaagcg atgatggcga ttgtactact cgaccatctg ttaagattta
     8101 aggctcaatg ccgttaaaca cataaaattg gaaaaatgaa attattaaaa acacttttag
     8161 tctcaagcct tgcttttaca gctaccgcac tgaatgcaac gcaatgggag aaaatcaaaa
     8221 ctccggttca aggaaaagca caatctatcg gcggttatag taacggctgt attattgggg
     8281 cacagcccct tgcattaaaa ggcgaaggct accaagtgat ccgctcggct cgtaaccgct
     8341 attacggaca cccacaactg ctcgattatc tcactcactt aggcaaaaaa accaaagctg
     8401 ccggtgtgcc gaccatttta atcggcgata tgggaatgcc tgccggtggg cgttttgcct
     8461 ccggtcacgc ctcccatcaa accgggctgg atgctgatat ttggtttaag ttcggaccga
     8521 tggacgatga aaccgcccgc aatccagcag ggcttgccac cattatggta aaccgtagta
     8581 ccgagcgggt tgatgattca ctctggaatc aacaccatac taatttagta aaacttgctg
     8641 caaccgatcc tcgagtggat cgcatttttg tcaatcctgc cattaaagtg aaactctgcc
     8701 aaaccgcagg caatgatcgt gcttggttac gcaaaattcg cccgtggtac ggacacgact
     8761 cccatttcca cgtccgttta acctgcccgg ctgatgccgc aagttgtgaa aatcaagccc
     8821 ttgtgcctgc cggtgacggc tgtggtgaag agctttactc gtggtttgag ccgaaagaag
     8881 cctccggcac accaaaagcc aaagtgctac ccacagcgcc tgttcaatgt cagatgattt
     8941 tatctgaaca aggcttgaat taattgcaaa ataacaacct tttttgaccg cttgtcagcc
     9001 cacaagcggt tcattttctt aaataattta cgattatgcg tttatttgtt gccgaaaaac
     9061 ccagccttgc ccgagcgatt gccgatgtgt taccaaaacc ccataccaaa ggagatggct
     9121 ttatccaatg cggcgaacac gacactgtta cttggtgtat cggtcattta ttggaacagg
     9181 ctgaacccga tgtctatgat gagcgtttca aaatgtggcg aatggaacat ttgccgattg
     9241 tgcctgaaca atggaaattg attcccaaaa aagagacgct caagcagttt aaagtggtgg
     9301 aaaagctgat taaaaaagcc gatgttttag tcaatgccgg agacccggat cgagaagggc
     9361 agctgttggt ggacgaagtt tttggctatt tgaatttatc tgctgaacgc aaaagccaaa
     9421 ttcagcgttg tttggtaagc gatctcaacc cgaatgcggt gagccgagcc attgaaaaat
     9481 tgcaaaaaaa cagcgatttt ataccgcttg caacctctgc tctcgcccga gccagagcgg
     9541 attggctgta tggtatcaat atgacccgag cctataccct acaggggcgt tgggcaggct
     9601 ataaaggcgt gttatcagtc ggacgtgtgc aaacgccggt attagggctg attgttcgcc
     9661 gagatctgga gatcgagcat ttcgtgccaa aggattattt tgaggtgttg gcacagattg
     9721 tcgtgccgga aacccaagag cgatttaagg cgcagtggca accgagcaaa gcctgtgaag
     9781 attatcaaga tgaagatggg cgggtgctgt ctcgtccatt ggcagaaaat gtggtaaaac
     9841 ggattaccga ccagccggca accgtcaccg aatatcaaaa taaaattgaa aaagaaactg
     9901 caccgctgcc ttattctctt tcggtattac aaatggaagc cgccggcaga tatgggctat
     9961 ccgcccaagc ggtgttggat atttgccaaa agctgtatga aacccataaa ctaattacct
    10021 accctcgttc tgataatcgg tatttgccga ccgaacattt caacgacaga tttaaagtga
    10081 tggcggcgat tgctcatcat ttacctgaat atcaacaaaa accggaagtg attgatgcca
    10141 ctattcgcaa tcgttgctgg aatgataaag aggtggaagc ccaccacgcc attattccca
    10201 ctgcacggca agggacggta catttaaccg aaaatgaaaa aagagtttat caactcattg
    10261 cccgccaata tctaatgcaa ttctgccctg atgcggaata tcgcaaaagt aaaatcgggc
    10321 ttgaaattgc aggtggtagt tttgtcgctc aagcacgcaa ccttgtaatt gccggctggc
    10381 gagcgttact gggtaaagaa gatagccttg acgatgcaga gccactacta ccgattgtca
    10441 aaaaagggca aatcctccat tgtgaaaaag gggaaattgt cagcaaaaaa acacaaccgc
    10501 cccgccactt tactgatcgc agtttgcttt cagcaatgac cgggatcgcc cgttttgtac
    10561 aagataaaga attgaaaaaa atcttgcgtg aaaccgatgg tttaggcacg gaggcaaccc
    10621 gagccggtat tatcgaattg ctgtttaaac gtggtttttt aatccgtaaa gggaaaaata
    10681 tccacagtac cgaagccggc agaatattga ttcaagcact tcccgactct gctacccagc
    10741 cggatatgac cgctcattgg gaaatgcagc tgaatcaaat cagcaaaaaa gagaccagct
    10801 atcagcaatt tatgtttgat ctaaaccaac agctcccaca tttattacaa agcccaaacc
    10861 gccaagtgtt gcaaaattta gcgaaaatca caccgcttgc aagcggcaat aaacctcgtt
    10921 attttaaaaa gcgaacacaa actgctgaaa attaaatcaa gcaggtaaaa attactgttt
    10981 agcacttgtc aaacgggcgt aaattctata ttatgcacgc cattgcaaat ttcgcataaa
    11041 gaatacaaga gaataaatta atgttaatta atgtactcac tattgtttat cttgtagttg
    11101 ccgtattact gatcgcattt gtattaatgc aacaaggtaa aggcgctgat gcaggtgctt
    11161 ctttcggggc gggtgcttca gcaacagttt tcggctcagc aggatcagca aattttttat
    11221 caagaacaac tgctctactt gcaacgattt tcttcgcaat tagtttagtt atcggtaatt
    11281 taagctcaca caatgtagca ccgaaaagcc aatttgaaga cttaagccaa gttgaacaaa
    11341 agcaagtaga acctgtaaaa atagaaaata gcgatatccc tcaataatcg cttaacatta
    11401 aaaagaataa agctctggtg gtggaattgg tagacacgcc atcttgaggg ggtggtggac
    11461 acagtccgtg cgagttcaag tctcgcccag agcaccattc aactaaattt ggctcaacgg
    11521 agctaaacta aaaaagcctt agaaagcctg taaaatcaac atttacaggc ttttttacta
    11581 tcttaactca acggctctaa acgtttccta tcacaaattt tagttacacg tttagttaca
    11641 cgttttaaat cgtgttacta tactttcaat aatcgtgtaa cacttcgtag gtttaattta
    11701 tggctagaac aactaaagca ttaaataaaa ctcaaataga aaatgccaaa tcaaaggata
    11761 aagaatatat tttatccgat ggcgatggat taaatcttag agttaaacct ctccctaaag
    11821 gctctaaaat ttggattttt aactacacta acccacaaac taaaaacaga acaaatttaa
    11881 ctattgggcg ttacccctta attacacttg cggaagctcg cactatatgt gatggctacc
    11941 gctcgttaat agagaaaggc atagatccac aggaagaaaa aaagcggctg caacaagaag
    12001 cagaaaatcg agtaaatgat actttccgga atgtggccga aagctatttc aatggtattt
    12061 ataaagagaa agcaaaaaat ccgattactc gtgaaaagaa ttggataagg ttagaaaatc
    12121 atattttccc ctatatcggg gataaacacg ttgaagaaat caaagtaaaa gcccttgttg
    12181 atatttattc taaagtggca gataaaagca acacattaaa aaaactgcat cagcttgtat
    12241 ctgctgtaat ggatcacgcc attacgcaag gcattataga aagccataac tgccgattag
    12301 cagttaaaaa cttttatatc aagccatcta cgccaaatcc cactattgag cttgaagaat
    12361 taccgcagtt atttactgat ttacataatg cgaagttatc aaagcaaact tacgcccttg
    12421 tttgttggtc gttcttgact gctctacgcc caaatgaagc cgttaatgct gaatggaaag
    12481 aaatagactt tgagaaaaag ctatggaata tccctaaaga gaagatgaaa ggaaaagcag
    12541 ataaaaaacg ccctcactct gtgccgttat cctcccaagc gttaaagata ttagagatga
    12601 tgaaaatgca ctctaattct agcccttttg tttttccagg tcgttcatca aatagtaaac
    12661 caatgagcaa cgcaacggta aattcaacat taaaaaggaa tggatataaa gatagactaa
    12721 ccgcacacgg tattcgggca tttgtgaaaa catttctatc ttctcgcaaa gtagatcgcc
    12781 acgtttccga aagtatatta tcccatttat tagaaggtgg agatgattta gaaaacacat
    12841 acaaccgata tgatttttta gaagagcgtg tgccagtaat gcagctttta ggtgattatt
    12901 gcgaacagtg cggaatgaat ttagagttag ttcggggtta gggaggtaaa atatatgatt
    12961 ttagtcgtca cgtcatcact tttcccactt tcccctttaa aatcaaatag ttaagtggtg
    13021 atgactggtt ttaaagtcat cattaagtca tcacaaaaag gtgatgacct agaaaaaaag
    13081 aggggtttcc ccctctttgc taattcaaaa tatcactttg aaagtcatca aaatttttga
    13141 aatgaacgtt tgttcgttgc cctttattcg tctttcgttt tgtgaaatcg tgcttattgc
    13201 cgtgttgctt tactgcttgc tcaataccta ctacaaaagt atttaagcct agttctagca
    13261 cattcttagc cttaacaaag gctaaatatg ccggataaag gtgcgttctt attttgtcgt
    13321 gtccaagcgt gttgccagtt ccgatataaa gcccgtctgt attttctgtt gtatatagat
    13381 actcgaaaaa atccgttaat gggtcagact gctttttaat ctccaacgcc tctaggctct
    13441 ctttttgctc ctctaaggct cttttagctt cgagtgggtc agggaaggta tcaagcactt
    13501 tacggataat gccccctatc tctaatgtaa ttttatccat aaaatacgga tcacgttcat
    13561 tttcaggcgg tcttttatta aaacggaaat tgacaatccg cctatctata ccgccattcc
    13621 gttctgtcca tttacaaggg tcgttattga cgatcataat cattgccgca attcttgcgt
    13681 taaatgggtg cttgttcttc ggatcgactc gcaccatatc accgccagta atcgctttca
    13741 atccgccacc atctccagcg tatttcgttt gctcggaaca gataatcagc tttttatctt
    13801 ccagccctgc gattttgcgt tcatcatcaa agttttctag ccttgctgaa gctgtgttct
    13861 tttctccagc taatagtgtc gcaatgccag caaataccga tttaccgctt ccgcctttcc
    13921 ctgttacttg gaagaatact tgccagttgt agcggtttgt taaaatcgca tagagagcgg
    13981 ctagaatgtt cttcgccttg tcttgcttgc cgtctgatac aaagttaagc cattgatcaa
    14041 agtgtggtgt ttcggctacg ttctcgtcat agctatgtgg aatataagcc gttagccagt
    14101 tttggcggtt aacggttctt tgaacattta ccgaataagg tgggtaaggg gttaaagttt
    14161 cgggttgccc cttgctattc caaccaatca ccgtagccgg ttgtacttcc attttttgaa
    14221 gcgtgccgaa tggtcgggtt ttatcgttgg ctgtcgtgcg tggataagct aagaagcctt
    14281 tagcttgttt tgtgcctgta ccgttggtta aatcggtttc ttcagtttcg acaaaggatt
    14341 ctgaaatttc ttcagtcagc cagcctagaa tatctacacc accaaagtct aaaatctctt
    14401 gcgtggtttt cgggtaagcg taaatattat gaacggcaat cgttacttca ttcattttcg
    14461 gggtggcggt ttcggtgcgt gcttcgcctt caccgatatg atttaccgct gcaccgccag
    14521 ccgatactaa ttttttatat tctttgcctt gggtcagttt caccacgtta gcaatttgtc
    14581 gcataacaga atcatctgtt aggcgtttca ttacttgctt gtcaagttcc ggaatcactg
    14641 aatagccacc gtctgcccct ccatttgtat tcgtagcaag cgaacgcaat tcgcctgttt
    14701 taatccagtg gcgaagctcg tcatcagata ctttcacatt tgcggtttca acctgttgcc
    14761 ccggttggtt acgctgttca tcagtaatcg cttcataacg tgcgatttct tttactaaat
    14821 tttcagcttg tgagcggagt tcatcaaact tagccccttc tgcttcagtt gttgagcgtt
    14881 tttcttcgtc tgctttggtt aaaattgaac gcatttctgc ttcaatagcc gctttttgtt
    14941 ggcggagttc taaaagtttt ttaaacattg ccttatcctt aattcaaata attgatattg
    15001 tagtgaagcg ttgctgtttt ccacgctgat tcgttagcat cataatcata gctatactta
    15061 gccagcacac attcgtccat actgtggaat gtggcttgat tcatcacatc actgattttt
    15121 tgagccacta aatctaggtc gttttcggtt gaaaacattg gtaagtaaat gccgattttc
    15181 aaataggcgt tgcattcgcc atcgcaaagc gtgagttggc tatattccgc ttcatcaatg
    15241 aatacggcga tagccggtaa ttcattttct acatcggtga atacggctcg gccgttatgg
    15301 aaatgttgaa tgccttgaat gttaccttgt aataagtcga taacttcttg gcggattgct
    15361 tcgttagaaa tcataaaaaa gtcctcttaa ttaagaaggc tacaattagc cacagaacaa
    15421 tataggcata atttcaacgg agtaaatagg gtaaaattta atcgttta
//
LOCUS       DANLXS010000042        15455 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.042, whole genome shotgun sequence.
ACCESSION   DANLXS010000042 DANLXS010000000
VERSION     DANLXS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15455
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.042"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            545..976
                     /locus_tag="PXD71_000966"
     CDS             545..976
                     /locus_tag="PXD71_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL6291108.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEPKKGNPFYACRIAAL
                     VGESDSPEYRYFDMNVVGKETETLIKRCQKAVSEEKKVLVSFVMADLWFDTFTYSTDS
                     KFHKKGDTGVSVKGRLIRIKMIKIDGEIAYQEKPKSDEQSE"
     gene            994..1986
                     /locus_tag="PXD71_000967"
     CDS             994..1986
                     /locus_tag="PXD71_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6291109.1"
                     /translation="MLCWALLLDVQDLRVTFKTPDGDVTAVNDLTFSLNAGETLGIVG
                     ESGSGKSQTAFALMGLLADNGRISGSAKFNGQEILNLPETQLNRLRAEQIAMIFQDPM
                     TSLNPYMKIGEQLMEVLMLHKGMDHKTAFEESVRMLDAVKMPESRKRMNMYPHEFSGG
                     MRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKSEFNTAIIMITHDLG
                     VVAGICDNVLVMYVGRTMEYGTAEQIFYTPSHPYSIGLIGAVPRVDGQEEELITIPGN
                     PPNLLRLPSGCPFQPRCVHATKQCLKAPALTLLNDQRLRACFVPAEQLIRGNAS"
     gene            1983..2972
                     /gene="oppF"
                     /locus_tag="PXD71_000968"
     CDS             1983..2972
                     /gene="oppF"
                     /locus_tag="PXD71_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDL6291110.1"
                     /translation="MNTEKKVILEVKDLSVHFNIKDTKSLFFAKHQTLKAVNHVSFQL
                     YEGETLGVVGESGCGKSTLARAIIGLVESSGGNILWLGKELTQQSAKEWKTIRSDIQM
                     IFQDPLASLNPRMTIGSIIAEPLKIYQPHLTAEEVKEKVRAMMMKVGLLPNLINRYPH
                     EFSGGQCQRIGIARALIVEPKLIICDEPVSALDVSIQAQVVNLLKSLQREMGLSLIFI
                     AHDLAVVKHISDRVLVMYLGNAVELGDYEQVYHDTKHPYTKALMSAVPIPDPQLERNK
                     QIQLLEGDLPSPINPPSGCVFRTRCPLADQHCIADKPLLKGRDNHVVACFKAE"
     gene            complement(3076..3834)
                     /locus_tag="PXD71_000969"
                     /pseudo
     CDS             complement(3076..3834)
                     /locus_tag="PXD71_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(3964..4605)
                     /gene="bioD"
                     /locus_tag="PXD71_000970"
     CDS             complement(3964..4605)
                     /gene="bioD"
                     /locus_tag="PXD71_000970"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDL6291111.1"
                     /translation="MAKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGC
                     RGIAEDLLIHRKIQGIELTEEDKQGITCPYVFDYPCSPHLAAKLEPRTIERKKIETCT
                     ALLCEKYDYVLLEGAGGLCVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQ
                     VLNSKRVSVHAVIYNLYPETDQVISQETQHFLRRYLEKYSPNTLFEIMDLISV"
     gene            complement(4623..5378)
                     /gene="bioC"
                     /locus_tag="PXD71_000971"
     CDS             complement(4623..5378)
                     /gene="bioC"
                     /locus_tag="PXD71_000971"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDL6291112.1"
                     /translation="MDRIDKGRIKQRFSAAVSNYDQQARAQQQIHQRLLSLLSYIDRQ
                     AFQRALEIGCGTGGLTKQLMSQLQVEHWELNDLCDMREHLQSVLPQPFHFHCGDAEQL
                     PFPQQYDLIASASVVQWFEDKAGFIARCKVRLKKHGFLLFSTFTPENLTQIKHLTGVG
                     LTYPRLEDWQKWLVPEFEILALEHEEIVLHFDDPLAVLKHLKQTGVTATQQQRWTKGQ
                     LHAFCQQYQQHYRNAQHKVELTYTPMYVLARLT"
     gene            complement(5382..6029)
                     /locus_tag="PXD71_000972"
     CDS             complement(5382..6029)
                     /locus_tag="PXD71_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HDL6291113.1"
                     /translation="MKTQHISQQGDNLIVYFAGWGTPPSVVEHLILPENYDLCLCYDY
                     RDLHCDVDFSAYQHIRVVAWSMGVWVAERVMQGYPLLSATAINGTGLPCDDQYGIPEA
                     IFAGTLSGLNEDNRTKFERRICGSKTLLRDYQHYSMRPTLQEIHAELTALYTFLQQDH
                     RTDLISWSKAIVGSKDKIFPAQNQRAYWQDRCAITEIAGEHLLFPHFTHWETCWL"
     gene            complement(6026..7189)
                     /gene="bioF"
                     /locus_tag="PXD71_000973"
     CDS             complement(6026..7189)
                     /gene="bioF"
                     /locus_tag="PXD71_000973"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDL6291114.1"
                     /translation="MIRYYQQQLADLKAQNQFRQLPQLIHRGRFIQREDNTMLNMSSN
                     DYLGLANNEALRQAFFTQYQDQLPALTSSSSRLLTGSFPIYDELESLMAQAFGRETAL
                     LFNSGYHANIGILPALADKKTLIVADKLVHASMIDGIRLAQCEFVRFRHHDYVHLEQI
                     LQKNDRTFERIIVVTESVFSMDGDCADLTQLVALKQRYPQVMLYVDEAHAIGVLGEKG
                     LGLAEQQGCINQIDILVGTFGKALGSMGAYVICDQVIRDYLVNKMRPLIFSTALPPFN
                     VAWTHFVFQQLPHLQAERAHLAQLSQHLRQAIVDIFQVPMPSESCIVPYILGDNELTV
                     RTAQRLQQQGYYCLPIRPPTVPKGTSRIRFSLTADMQVAEVEQFIACLRELAE"
     gene            complement(7186..8487)
                     /locus_tag="PXD71_000974"
     CDS             complement(7186..8487)
                     /locus_tag="PXD71_000974"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDL6291115.1"
                     /translation="MNEQQLLECDRQHIWHPYAALNADFPLFAVERAEGVYIQLKNGQ
                     QLIDGMSSWWSAIHGYNHPRLNHAATQQLSQMSHVMFGGLTHQPAVELAQQLVNILPE
                     SLDKIFFADSGSVAVEVAMKMAVQYQHAKGETQRYKFATIRSGYHGDTWHAMSVCDPV
                     TGMHSLFAKGLPVQYFLPQPPVKFGQAWQPEAMQPLQDLLAQHGDEIAGLILEPVVQG
                     AGGMYFYSPEYLVQAKALCEPYGILLIFDEIATGFGRTGKLFACEHANVVPDVMCIGK
                     ALTGGYLTLSATITNQAVADVICSGEAKCFMHGPTFMANPLACAVAAESIKLLLASPW
                     QQRIQQIEQQLRQELRQAEALDCVAEMRVLGAIGVIEMKAPVNMASLPPRFVQNGVWV
                     RPFGKLVYLMPPYIISSDQLSQLIHGTIRTLQEEYAHKESL"
     gene            8629..9516
                     /gene="psd"
                     /locus_tag="PXD71_000975"
     CDS             8629..9516
                     /gene="psd"
                     /locus_tag="PXD71_000975"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755522.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL6291116.1"
                     /translation="MNILEKKAQLSYWQRIKIAFQYVMPQLYLTRLAGWFAKQQWGAV
                     THFVIKLFAKKYHVDMSEAAKPNFSDYASFNEFFIRPLADNARPINQNPTALCLPADG
                     RISQLGHIEQDLLLQAKGHYFSLNDLLAGDEALAHHFKDGEFATTYLSPRDYHRVHMP
                     CDATLCKMIYVPGDLFSVNPFLAEHVPNLFARNERVICVFDTEFGKMVQILVGATITA
                     SMSTVWAGVINPPRPEKITTWAYEGESAVKLLKGQEMGAFQLGSTVINLFEKDRVQLA
                     SHLQVDSPVRMGEILAHQK"
     gene            9541..10095
                     /locus_tag="PXD71_000976"
     CDS             9541..10095
                     /locus_tag="PXD71_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6291117.1"
                     /translation="MSQQFFDQVSLESQLEVSAEAVAKGIFDVLNQNPPALELNEVSH
                     ALQTLQQQAKFKEIEAVGAQFLAENAQKAGVNTTESGLQYEVLVAGEGQIPAREDKVR
                     VHYTGTLIDGTVFDSSVKRGQPAEFPVNGVIAGWIEALSMMPVGSKWRLTIPHNLAYG
                     ERGAGASIPPFSTLVFEVELLAIL"
     gene            complement(10155..10478)
                     /locus_tag="PXD71_000977"
     CDS             complement(10155..10478)
                     /locus_tag="PXD71_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291118.1"
                     /translation="MNWKAVLIFSVLGSLVSCASYSEHAVQRIEAGKSFAVTGNTKRI
                     NTMACQDNDDWYLDGYRVGKSFREHQQKMLIQRTAYCEEQTGKAVPDKFRHSWNSGYQ
                     QGLKR"
     gene            complement(10502..11605)
                     /gene="proB"
                     /locus_tag="PXD71_000978"
     CDS             complement(10502..11605)
                     /gene="proB"
                     /locus_tag="PXD71_000978"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL6291119.1"
                     /translation="MKIDKTLVVKFGTSTLTQGSAKLNLPHMIDIVRQLAQLHQAGFR
                     LVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQSQLIQTWEKLFAIYDIHIGQIL
                     LTRADIEDRERFLNARDTLRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILV
                     QAEQLYLLTDQQGLFDRDPRKHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKI
                     SAADVATRSGIETIIAPGNRPNVIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAG
                     IITIDAGAENAMLIQHKSLLPAGIVNVEGRFSRGEVVKIRTQQGKDVALGMPRYNSDA
                     LHLIQGKHSQDIEQVLGYEYGAVAVHRDDMIVL"
     gene            11771..12759
                     /locus_tag="PXD71_000979"
                     /pseudo
     CDS             11771..12759
                     /locus_tag="PXD71_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
     gene            12834..13481
                     /locus_tag="PXD71_000980"
     CDS             12834..13481
                     /locus_tag="PXD71_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDL6291120.1"
                     /translation="MTINTYAKFAPTVFVAKCSESHTKGEIITLTSKYGNETEVQVHN
                     LVKQDGEHYFYSFTRCDGMDSQSFAQQKAERYQGYADNAAKRSQDWVNAANEGCDFLS
                     LGEPIKIGHHSEKRHRNLIERNARRMDNAVAEMNKAASYESRIAYWEKMAGKIDLSMP
                     ESLEYFKFELDKAKTKHKELKDNPEKREHGYSLTYAKKAVNELEKKVKLAELLWA"
     gene            13505..13912
                     /locus_tag="PXD71_000981"
     CDS             13505..13912
                     /locus_tag="PXD71_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL6291121.1"
                     /translation="MNRQGIYINGKQVFDSQGEDIQSIGFFGCNIQTVVGINKGSILV
                     SSNSNQLLVNGQSIELGSEPVLKIEVLGNVQKLSAGAADVSITGDAGSVDTVSGDVSI
                     KGNVASYVKTVSGDVRCRAITGSVETVSGDIYR"
     gene            14010..14360
                     /locus_tag="PXD71_000982"
     CDS             14010..14360
                     /locus_tag="PXD71_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291122.1"
                     /translation="MTTINLSQQERELVEFVWNNRNTPWFFHCSVSNVSRNNTSRQIA
                     VCVIKDNQLISLDELIAKLCGYKLRQNGLYVTVHNMDAVFDTLYRFMCKLNDEMEANE
                     NVCPYLSLAHYEVF"
     gene            14451..14813
                     /locus_tag="PXD71_000983"
     CDS             14451..14813
                     /locus_tag="PXD71_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291123.1"
                     /translation="MIDTFFDLDDTSCYLGNWEDILKEYDDCLSVEDFWHSAKEFDTV
                     PHFGNLYQERVILKLIARFCIELNIEQDSGLVEFDYYINAIDTHFYINSQRICDIDDW
                     NEMLDKIREEMTPAKLVA"
     gene            14926..15186
                     /locus_tag="PXD71_000984"
     CDS             14926..15186
                     /locus_tag="PXD71_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291124.1"
                     /translation="MTFELGRVCATRAVAECFNQHTLVDLLHRYCSGDWGNLCDNDKK
                     ANVKAIERGGRILARYDVEDETLYVITEADRSYTTILFPYEY"
BASE COUNT         4493 a         3311 c         3169 g         4482 t
ORIGIN      
        1 aaagggtgcc gtttgtgtcc tcgatgatgc cacagatatg ccgattaaag accacaaggt
       61 aatcgggtaa attacgaacg aaaatatcaa cattgtcacg aaggtgctgc tattttcaat
      121 atagtaactt atattggtct aacttaaatt tcatttattc ttcaaaagta caacaccgtt
      181 ggtaagtgat ttatcaacgg tgttgtcatt tccaattctc taaaatcaaa attcaattct
      241 caattaactt tatgattcaa tatgactaaa ttatcctcaa tttaagacgc tgtatataac
      301 agcacttaat aagtcgcata actcctgaga agcgacggct atccttagag gtagttgagt
      361 tattgccaaa atgtctccta gcattctgaa agggaacgcc acttttgtgg gaatgccacc
      421 taaaccctct accatctcag tgaagcatca ttgagatttc attttacttc cacctcgcgg
      481 ggaacatttt tcccgtgttg ggcaaggatg tttctccgct tttttacatt tggagaaaca
      541 aactatgaca actcaaactt ctactcaaaa aaattacttc aaccttcata caacaggcat
      601 tggctatcta aacgatattc gtttggtaga gcctaaaaaa ggaaatcctt tttatgcctg
      661 ccgtatagca gctctggttg gcgaatctga ctctcctgag tacagatatt ttgatatgaa
      721 tgtagtcggt aaagagactg aaacacttat caaaagatgc caaaaagcag ttagtgaaga
      781 gaaaaaagtg cttgtgtcat ttgtaatggc tgatttgtgg ttcgatacat ttacttactc
      841 aacagatagt aaattccaca aaaaaggcga taccggtgta tctgtaaaag gtcgcttaat
      901 ccgcatcaaa atgattaaaa ttgatgggga aatagcctac caagaaaagc caaaatcaga
      961 tgagcaatct gagtaatttt acctaagccc gacattttat gttgggcttt attactcgat
     1021 gtacaagatt tgcgcgtaac ttttaagacc ccagatggcg atgttactgc cgtcaatgat
     1081 ctgacttttt ctttaaatgc cggtgaaacc ctcggcattg tcggcgaatc tggctcagga
     1141 aaatcgcaaa ccgcttttgc tctaatgggg ctattagcag ataacgggcg aatttcagga
     1201 tcagcaaaat ttaatggaca agaaattctc aacttgcctg aaacacaact taaccgcttg
     1261 cgtgctgaac aaattgcgat gatcttccaa gatccaatga cctcgctgaa cccttatatg
     1321 aaaatcggcg aacagctaat ggaagtactg atgttgcata aaggcatgga tcataaaact
     1381 gcgtttgaag aatcggtacg gatgttagat gcggtgaaaa tgccggagtc acgtaaacgt
     1441 atgaatatgt atccgcatga attttccggc ggtatgcgtc agcgtgtgat gattgcgatg
     1501 gcattacttt gccgtccgaa actcctcatt gcggatgaac ccacaactgc gctcgatgtg
     1561 acggtgcaag ctcaaatcat gaccttatta aacgaattaa aaagcgagtt taataccgcc
     1621 attatcatga ttactcacga tctcggtgtg gtcgcaggaa tttgcgataa tgtgttggtg
     1681 atgtatgtag gcagaaccat ggaatacggc accgctgagc aaattttcta taccccaagc
     1741 cacccttatt ctatcggctt aattggtgca gtgcctcgcg ttgacggaca ggaagaagaa
     1801 ctcattacta ttccgggtaa tccacctaac ttattacgct taccgagtgg ctgtcctttc
     1861 caaccacgtt gcgttcatgc aactaaacag tgcctcaaag caccggcgtt aaccttgtta
     1921 aatgatcaac gtttacgcgc ttgttttgtc cctgccgagc aattaatccg aggtaacgca
     1981 tcatgaacac agagaaaaaa gtgatcctag aagtgaagga tttaagcgtt catttcaaca
     2041 tcaaagatac gaaatcttta ttctttgcta aacaccaaac tttaaaggcc gtcaatcatg
     2101 tctcttttca gttatacgaa ggcgaaacct taggtgtggt cggtgaatcg ggctgtggaa
     2161 aatcaactct cgcacgcgcg attattggct tagtggaaag ctctggcggt aatattttat
     2221 ggctaggtaa ggagttaact caacaaagtg cgaaagaatg gaaaacgatc cgtagcgata
     2281 ttcaaatgat tttccaagat cctttagcct ccctgaatcc acggatgacc attggcagta
     2341 tcattgccga accattaaaa atttaccaac cccatttaac cgcggaagaa gtgaaagaaa
     2401 aagtacgcgc gatgatgatg aaagtgggct tattacctaa cttaatcaat cgctatccac
     2461 atgaattttc gggtggacaa tgtcagcgta tcgggattgc gcgcgccttg attgtggaac
     2521 caaaactcat tatttgcgat gagccagtgt ccgccttaga tgtttcaatc caagcacaag
     2581 tagtgaattt attgaaatct ttacaacgcg aaatggggct atcattgatt ttcattgcac
     2641 acgaccttgc tgtggtgaaa cacatttccg atcgtgtttt ggtcatgtat cttggtaacg
     2701 cggtggaatt aggtgactat gaacaagtct atcacgatac taagcatcct tataccaaag
     2761 ctttgatgtc tgcggtaccg attcctgatc cgcaattaga acgcaataaa caaattcaat
     2821 tgcttgaagg cgatctccct tccccgatta atccgccgtc gggctgtgtg ttccgcaccc
     2881 gttgcccact ggcagatcaa cactgtattg cagacaaacc gctcctcaaa ggacgagata
     2941 atcacgttgt tgcttgtttt aaagccgagt aatacacaca accgcacaaa aaagtgcggt
     3001 tgtttttttg cgaatattca cagtcagact ttcgtgatac tttcgctctg agataaggat
     3061 tacgatacag agaagttatt caacaatcac acgtgcattg atcggttgca ctggacgatt
     3121 attgtgtcct ttcatcaagg tcgcaaacgc ctcaacttga gattggctaa tgttgtcata
     3181 gtgttttaac actaaccaat ttacaccttc agaacaaggt ggtgtagtta atgacccgct
     3241 gaaacgatag taatcgcgtt ttttcggaat taatgttgct gggtcaaaag cggtactaag
     3301 cgtatatttc tcccctgctt tttgcgggaa gttttgccaa attggggcta accattggtt
     3361 ttcacttgat aatacataga gcacgccaag cacagcaaga ttcccttcat tgtctgcgtg
     3421 tacaaaatgt gcttccatcg ggaaggattt acctcaaatt aagttttcac tcggcacatg
     3481 gaaatggaat tgtttaaggg taaaagtctt tccattaagc tgcatgcgat tacttgcctc
     3541 tttgaaatcc acttgaatag tatgcccatt attgacaatc tccacattgg aaggtcgata
     3601 atccagctta atcggcgcta acttaccatc aatcgtatgc ttgatatcta ttggtgtttg
     3661 gtttttacct tcgccacaaa tacggaattt gggtgaaagt tcagcccaat gttctggcga
     3721 ttcgtgtccg gtgtatcccc aatgcgtgtg cgtttcttct ttcttaaggg ggcttgtaca
     3781 cgctgtcaat gctaatgcac ctaacgtaag cacaaaaagg tttgattttt tcatagccta
     3841 gctcctgatt aaaagagatg aattgatgag acaacctaaa ctcaatgatg ccttaatccg
     3901 agacgctttc atcttaggca aaacaacact ccatttcaag tataacttgc tcaagaatat
     3961 atcttagacc gaaatcaaat ccattatttc aaataaggta ttaggcgagt atttctctaa
     4021 ataacgacgt aaaaaatgtt gtgtttcttg gctaatgact tgatctgttt ccggatacaa
     4081 gttgtatatc acagcatgca cacttactct tttggagttg agtacttgta aactcaataa
     4141 ggtatgatta atactaccta gcttgccaga ggtaactaaa atcacggggt attgatgctg
     4201 acaaagataa tcaagggtgg tttcttcctc attataaggt acacacaagc cgcccgcccc
     4261 ttccaataat acataatcat atttttcaca caaaagtgcg gtgcaggttt caattttttt
     4321 acgttcgatc gtgcgcggtt ctaatttcgc ggctaaatgc ggtgagcaag gataatcaaa
     4381 cacgtaagga caagtgatac cttgcttatc ttcttctgta agctcaatac cttgtatttt
     4441 tcggtgtatg agtaaatctt cggcaattcc tcggcagcct gtttggatca tcttttgcgt
     4501 gatgacactt gcgccttgtt gcatcagttt cttggcgtac caacctgtgg cgatggtttt
     4561 gccaatatcg gtgtcgatgc cagtgataaa aaaaaccttt gccatacttt ttccttctaa
     4621 gattaagtta aacgcgccag cacatacatc ggcgtgtaag tgagttctac tttgtgttgt
     4681 gcgttacggt aatgttgttg atattgttgg caaaaggcgt gcaattgccc ttttgtccac
     4741 ctttgctgct gtgtggcagt aacgcctgtt tgtttcaaat gttttaacac cgctaaggga
     4801 tcatcaaagt gcaacacaat ctcctcgtgt tcgagggcta aaatctcaaa ttcaggcact
     4861 agccactttt gccagtcctc aagacgtgga taagttaacc ccacaccggt gaggtgtttg
     4921 atctgtgtta aattctcggg cgtgaaagta ctgaataaca aaaatccgtg cttttttaag
     4981 cgcactttac aacgcgcaat aaaacccgct ttatcttcaa accattgcac cacggaagca
     5041 ctggcaatca aatcatactg ctgcggaaag ggaagttgtt ccgcatcgcc acaatggaag
     5101 tgaaaaggtt ggggcaacac tgattgcaga tgctctcgca tatcgcaaag gtcattcagc
     5161 tcccagtgtt caacctgcaa ctgtgacatt aattgcttcg ttaatccacc agtaccacat
     5221 ccaatctcta atgcacgttg gaatgcttgt ctgtcgatat acgacaataa actaagcaaa
     5281 cgttggtgaa tctgctgttg tgctctcgct tgctgatcat agttcgatac agcggcagaa
     5341 aaacgctgtt taatgcgtcc tttatcaata cgatccatgt gctacaacca acaagtttcc
     5401 caatgggtga agtgaggaaa caacaaatgc tcccctgcga tttctgttat tgcacaacga
     5461 tcctgccaat aagcccgctg attttgtgca gggaaaattt tatcttttga gccgacaatc
     5521 gccttgctcc acgagattaa atctgtacgg tgatcttgtt gtaaaaaggt ataaagtgcg
     5581 gtcagttcag catgaatttc ctgtaatgtg ggacgcatgg aatagtgctg ataatctctt
     5641 aacagcgttt ttgaaccaca aatacgacgt tcaaatttag tccgattatc ttcatttaaa
     5701 ccagataacg tgccagcaaa aatggcttcg ggaataccat attggtcatc acaaggtaag
     5761 cccgtgccat tgatcgccgt agcagatagc agtggataac cttgcatcac acgctccgct
     5821 acccacacgc ccattgacca tgccaccacg cgaatatgtt gataagcgga aaaatcaaca
     5881 tcgcaatgca gatctcgata atcatagcaa aggcataaat cataattttc cggcagaatc
     5941 aggtgctcga ccacgctcgg cggtgtgccc caccctgcaa aatagacgat cagattgtcg
     6001 ccttgctgtg aaatatgttg tgttttcatt cagcaagctc ccgtaaacaa gcaataaatt
     6061 gttccacttc cgccacttgc atatctgcag tcagtgaaaa acgaatacga gaggttcctt
     6121 ttgggactgt cggcgggcga atcggtaagc aataatagcc ttgttgttgc aaacgttgcg
     6181 ccgttcttac tgtcagttcg ttatcgccca aaatataagg tacgatacaa ctttcacttg
     6241 gcatcgggac ttgaaaaatg tcaacaatcg cttggcgtaa atgttgacta agctgagcaa
     6301 gatgtgcgcg ttcggcttgc aaatgtggta attgttgaaa gacaaaatgc gtccatgcca
     6361 cattaaacgg cggaagtgcc gtggaaaaaa tcaatgggcg cattttatta accaaataat
     6421 ctcggatcac ttgatcgcaa atcacataag cccccataga gcccaacgcc ttgccaaacg
     6481 taccgactaa aatatcaatt tggttgatac agccttgttg ttctgctaac cctagcccct
     6541 tctcccccaa tacgcctatc gcatgggctt catctacata taacatcact tgcggatagc
     6601 gctgttttaa tgcgaccaac tgggtgaggt ctgcacagtc accatccata ctaaacacgg
     6661 attccgtgac cacgataatc cgctcaaaag tgcgatcatt tttttgcaaa atttgttcaa
     6721 ggtggacgta atcatgatga cgaaaacgca caaactcaca ttgtgccaag cgaatgccat
     6781 caatcatact ggcatgtacc agtttatccg ccacaatcag cgtttttttg tccgctaaag
     6841 caggcaaaat cccaatatta gcatgataac cgctattaaa tagcaaggca gtttctcgtc
     6901 caaatgcctg cgccattaag ctttctagct catcataaat cggaaaagag cctgttaata
     6961 aacgggagga agaagaagtc aatgcaggga gttgatcttg gtattgcgtg aaaaatgcct
     7021 gacgcaaggc ttcgttattg gctaaaccta aataatcatt ggaggacata ttcagcatcg
     7081 tattgtcctc acgttgaata aaacgccctc ggtgaattag ttggggcagt tggcgaaatt
     7141 gattctgcgc ctttaaatca gctaactgct gctgataata cctgatcata gagattcctt
     7201 atgtgcatat tcttcttgaa gggtgcgaat tgtgccatga attaattgag acaattgatc
     7261 cgatgagatg atatacggtg gcatcaaata cactaatttg ccaaatggac gcacccaaac
     7321 gccattttgc acaaagcgtg gtggtaaact tgccatattg acaggcgcct tcatttcaat
     7381 caccccaatc gctccaagta ctctcatttc agcgacacaa tctaaggctt ccgcttggcg
     7441 caattcttga cgtaattgct gttcaatctg ttgaatacgt tgttgccaag ggcttgccaa
     7501 taacaatttg atactttctg ctgctaccgc acacgccagt ggatttgcca taaacgtcgg
     7561 accgtgcata aaacattttg cctcgccaga acaaatcacg tctgctaccg cttggttggt
     7621 aatggtagcg gatagagtta aatacccccc cgttaaagct ttaccgatac acatcacatc
     7681 cggcacgaca ttggcatgtt cacaagcaaa taatttgcct gtacgcccaa atcctgtggc
     7741 aatttcatca aaaatcagca aaatgccgta cggttcgcat aaggcttttg cttgcaccaa
     7801 atactcaggg gaataaaaat acataccacc cgccccttgc actaccggtt ctaaaattaa
     7861 acccgcaatc tcatcaccat gttgtgccaa taaatcctgt aacggttgca tcgcttcggg
     7921 ttgccaagct tgcccaaatt ttaccggggg ttgcggtaaa aaatattgca cgggtagccc
     7981 tttggcaaac aagctgtgca tgccggtcac gggatcacac acggacatcg catgccaagt
     8041 atcaccatga tagcccgaac gaatcgtagc aaatttatag cgctgtgttt cgcctttagc
     8101 atgttgatat tgcactgcca ttttcatcgc cacttccacg gcgaccgaac cactatcggc
     8161 aaagaaaatt ttatccaaac tttccggcaa gatgttgacc agttgctgcg ccagttctac
     8221 cgctggttgg tgcgttaatc caccaaacat cacatgactc atttgactca gttgttgcgt
     8281 ggcagcgtgg tttaaacggg ggtgattata gccatgaatc gctgaccacc acgatgacat
     8341 gccatcaatc aactgctgac cgttttttaa ctggatatac acaccttcgg cacgttctac
     8401 tgcaaataag ggaaaatccg catttaacgc cgcataaggg tgccaaatat gttgtcgatc
     8461 acattctaaa agttgctgct cattcatcgt ttcacctcgg ttgtgaatcg ccctttcaac
     8521 tggcgtaaat tcacgcaaat acttacataa acggaataac ttgttataat cgccaagcat
     8581 ttggcttttc aagcccatta ttctaactta aaaagatgat ttattactat gaatatatta
     8641 gaaaaaaaag cacaactgag ttactggcaa cggattaaaa ttgcctttca atatgtgatg
     8701 ccacaacttt atttaacacg tcttgcaggc tggtttgcaa aacagcaatg gggtgctgtc
     8761 acacattttg tgattaaatt attcgcgaaa aaataccatg tggatatgag tgaagctgca
     8821 aaaccaaatt tcagcgacta tgcgagcttt aatgaatttt ttatccgtcc attagcggac
     8881 aatgcccgtc caattaatca aaatcctacc gcactttgtt taccggcaga tggtcgtatt
     8941 agtcagttag gacacattga gcaggattta ttgctacaag ccaaaggaca ttatttcagc
     9001 ttgaacgatt tgttagctgg cgacgaggcg ctcgctcatc attttaaaga tggcgaattt
     9061 gccaccacct acctctcacc acgcgattac caccgtgtgc atatgccttg tgatgccacc
     9121 ttatgcaaaa tgatttatgt gccaggtgac ttattctcag tcaatccatt tttagccgaa
     9181 cacgtgccaa acttatttgc acgtaatgaa cgcgtgatct gcgtattcga taccgaattt
     9241 ggtaaaatgg tccaaatttt agtcggcgca accattaccg cgagtatgag caccgtgtgg
     9301 gcaggtgtga tcaacccgcc tcgtccagaa aaaatcacca cctgggccta tgaaggagaa
     9361 agtgcggtca aattactgaa aggacaagaa atgggagcct ttcaattggg ctcgacggtg
     9421 atcaatttat ttgaaaaaga tcgtgtgcaa ctggcatctc atttacaagt ggatagcccg
     9481 gtacgtatgg gtgaaattct tgcccaccaa aaataatttt taaccacaat aggaaaaaca
     9541 atgagtcaac aatttttcga ccaagtgtca ttagaaagcc aacttgaagt gagcgccgaa
     9601 gccgtcgcga aagggatctt tgatgtgtta aatcaaaatc caccagcact tgagttaaat
     9661 gaagtgagtc atgccctaca gactttgcaa caacaagcga aattcaaaga aattgaagcc
     9721 gttggtgcac aattcttagc tgaaaacgcg caaaaagcgg gggtaaatac tacggaatct
     9781 ggtttacagt atgaagtgtt agtggctggt gaagggcaaa ttccggcacg cgaagacaaa
     9841 gtgcgtgtgc attacactgg gacattaatc gacggtaccg tctttgacag ctcagtaaaa
     9901 cgtggtcaac ctgcagaatt cccagtaaat ggcgtgatcg ctggttggat tgaagccttg
     9961 tccatgatgc cagtgggttc aaaatggcgt ttaaccattc ctcacaacct cgcttatggc
    10021 gaacgtggtg ctggtgcctc cattccgcca ttcagtacct tagtgtttga agtcgaatta
    10081 ttagccattc tttaaagcta aacgctagac ataaaaaagt gcggtcattt tccgcacttt
    10141 tttatgtcta gcgtttatcg tttcaagcct tgctgatagc cgctgttcca cgaatggcgg
    10201 aacttgtccg gcacagcctt gcccgtttgt tcttcacagt aagccgttcg ttgaatcaac
    10261 attttttgtt ggtgttctcg aaatgatttt cccacacgat agccgtccaa ataccaatca
    10321 tcattatctt gacatgccat ggtattaatc cgttttgtat tccccgtcac tgcaaaagat
    10381 ttgcctgcct caatacgttg caccgcatgt tcagagtatg aagcacagct caccagcgaa
    10441 cctagtacac tgaatatcaa tactgctttc caattcatct tgttaccttt ctttttctga
    10501 tttataacac aatcatatcg tctcggtgta ccgcaaccgc cccatattca taaccgagaa
    10561 cctgttcgat atcttgcgaa tgtttgcctt gaattaaatg gagcgcatcg ctgttataac
    10621 gtggcatccc taaggcaaca tccttgcctt gctgagtacg aatttttacc acttcaccgc
    10681 gtgaaaagcg cccttccacg tttacaatcc cagcaggcaa taacgattta tgttgtatta
    10741 acatcgcgtt ttccgccccc gcatcaatgg taataatacc ggctgacggc gccgcatata
    10801 gccattgttt acgactttct aagcgatctg cttgcacact aaatttggtg ccaatggctt
    10861 gcccataagc taagtccaca atcacattcg ggcgattacc cggtgcaata atggtttcaa
    10921 tgccggagcg ggttgccaca tccgcggcac tgattttggt ggacatgcca cctgtgccta
    10981 acgaggtgcc acttccgccc gcaatcgcgc gaatatgatc ggtaattttg tccacgtcgg
    11041 cgatcaattt ggcgtctgga tgtttgcgcg gatcacgatc aaataaacct tgctgatcag
    11101 taagcaaata caactgttct gcttgcacca aaattgccac taacgcggat aaattatcgt
    11161 tatcgcctac tttaatttct gacgtagcga cagcgtcatt ttcattaatc actggaataa
    11221 tctgattgtc taataaggcg cgcaaagtat ctctggcatt taaaaaacgt tcacgatctt
    11281 caatgtcggc acgggtcaat aaaatctgcc caatatgaat atcataaatg gcaaacaatt
    11341 tttcccacgt ttgaatgagc tgactttgtc ctaccgcagc caataattgt ttcgaggcaa
    11401 tcgtcggtgg caattgcggg tgatttaaat aatgacgacc tgccgcaatc gcccctgagg
    11461 taacaatcac taaacgaaag cccgcttgat gaagctgtgc gagctgacgc acaatatcta
    11521 tcatatgagg taaatttaac ttcgccgatc cttgcgtaag cgtactggtt ccaaatttta
    11581 caacgagtgt tttgtcgatt ttcatcttct ctatcttctt atttttgtcg cacaaaattg
    11641 aagctaactt accattaatc atcagaaaaa tcatctcgtt tacttgctat tttctatgat
    11701 ttagcacaaa ttttttctca ctaaaaggca tataataaag cttcttgatt tttcagctag
    11761 gaacccgatt atgacctcaa cctcttcaca agaaacggag cggcaacgag aaatcactcg
    11821 cctttgtgtt caagttgccc aactcttata caacatggtg cagaaagtgc catcgttgtg
    11881 caaatggcac atcgcttagg cttagccctc ggtgtagata gcgtagaatg tgccctcacg
    11941 ccaaatgcca ttattcttac tacattagcc caagaccatt gcatcacgac agtccgtaaa
    12001 aaccaagaca aaggaattaa tatgcaagtg gttaccgaag tgcaacgtat tgtgattaca
    12061 gcagaacgta aaatctatga tatgcaacaa gtgcatgcca aactggctcg cattaaaccg
    12121 cttaaataca atcgctattt tgtgatgttg atgattggcg tatcctgtgc cagttttgct
    12181 catttatctg gtggggacgc actagtttca ttggttacat ttctcgccgc aacactcgcc
    12241 atgtatgttc gccaatcact tgccgcaaga cactataacc ccttaattgt atttgcggtg
    12301 accgcctttg tcgcctcact aattgcgggt atcgcgttga aatataacat cggcaatcag
    12361 ccccaaattg ccttggcatc cagcgtatta ttgctcgttc ccggctttcc actgattaat
    12421 gcactcgccg atattttaaa agggcatatc aatatgggga ttgcccgttg ggcactcgcg
    12481 acaatcctca catttggggc atgcttaggc attgtatttg cgctgagttt attcttagag
    12541 gaaatctgta tgctaaataa attcattcaa gatgaatttc tacggctaga tcaacgttat
    12601 gcagcttggt atcaaagtga agaaacggat gaagttgcca gaatggggat tcaagaaagt
    12661 gagcggattt tgaaaattct gtatgcactg aaatataacg aagaccctat gcaaaatggt
    12721 ttaggctata ttgcatttat ggcatcaaaa ggccaataat ttacttatct caacaattta
    12781 cccaccgggg aaactcctcg atggcgtttc ccctacttta taggagaaac gctatgacta
    12841 ttaacactta tgcaaaattt gcacctaccg tttttgttgc aaaatgctct gaatcacata
    12901 ccaaaggtga aattattacg ctaaccagca aatatggtaa tgaaaccgag gttcaagtac
    12961 ataatcttgt taaacaagat ggcgagcatt atttttattc atttactcgt tgtgatggga
    13021 tggatagtca gtcatttgct caacaaaaag cagaacgcta tcaaggctat gcagataatg
    13081 cagccaaacg tagtcaagat tgggtaaatg ccgctaatga ggggtgtgat tttttgagtt
    13141 taggtgagcc aataaaaatt ggtcatcaca gcgaaaagag acaccgtaac cttattgaac
    13201 gtaatgctcg ccgtatggac aatgcggttg cagaaatgaa caaagctgca agttatgaat
    13261 ctcgtatcgc atattgggaa aaaatggcag gaaaaattga cctgtctatg ccggaaagtc
    13321 tggaatactt taagtttgaa ttggataaag ccaaaacaaa gcacaaagaa ttgaaggaca
    13381 accccgagaa acgagagcat ggttattcac tgacttatgc taaaaaagca gttaatgaac
    13441 ttgagaaaaa agtaaaatta gctgaattac tttgggcata attatttaca aaggagatga
    13501 cgatatgaat cgacaaggta tttatattaa tggcaaacag gtgtttgatt cacaaggcga
    13561 agatattcaa agcattggat tttttggctg caatattcaa acggtcgtag gcataaacaa
    13621 aggaagtatt ttagtctcgt ccaatagtaa tcaattattg gtgaatggac agtcgattga
    13681 attaggttct gagccagtcc tcaaaattga ggttttgggc aatgtgcaga aactgagtgc
    13741 cggtgccgca gatgtctcta ttacaggaga tgccggcagt gttgacacag ttagcggtga
    13801 tgtctctatt aagggaaatg ttgcctctta tgtgaaaacc gtgagtggtg atgttcgctg
    13861 tcgtgctatt accggttcgg tggaaaccgt ctccggtgat atttatcgct gatatttact
    13921 catcttatta tttaccaccc tattgggaag caattttccc aaggtgggca gattgtttcc
    13981 catttttatt ttgaatttgg agaaacatga tgaccactat taacctttca caacaagaac
    14041 gtgagttagt tgaatttgtc tggaataacc gtaatacacc ttggtttttt cactgtagtg
    14101 tatcgaatgt gtcgagaaat aatacctctc ggcaaattgc tgtatgtgtc attaaggata
    14161 atcaattgat tagccttgat gaattgatag caaaattatg tggttataaa ctcagacaaa
    14221 acggattata cgttactgtt cataatatgg atgctgtttt tgatacctta tatcgcttta
    14281 tgtgcaagct aaatgatgag atggaggcaa atgaaaatgt ttgcccttat ttatcattag
    14341 ctcactatga agtgttttga ttggtatcag aactatccac cctgtcggga aacaattttt
    14401 ccgatagggc agattgtttc ccacttttat tttgaatttg gagaaacata atgatagaca
    14461 ccttttttga ccttgacgac acctcatgct atttaggtaa ctgggaggat atactgaaag
    14521 aatatgatga ttgtctttca gttgaagatt tttggcattc cgctaaagaa tttgataccg
    14581 tacctcattt tggcaaccta taccaggagc gtgtcatcct taagttgatt gctcgattct
    14641 gtattgagct gaatattgag caagattcag gtttagtcga atttgattat tatatcaatg
    14701 caatagatac gcatttctat attaatagcc aaagaatctg tgatatagat gattggaatg
    14761 aaatgttaga taagattcgt gaagaaatga cgccggctaa attagttgcc taatttttgc
    14821 aaatttttac taaaaatgac gcttactgtc gtcttaattc acccacttgg ggaaatatct
    14881 ccccaatggg atgacatttc ccctctttct gtttaggaga aatgtatgac ttttgagctt
    14941 ggtagagtat gtgcaactcg tgcagttgct gaatgtttca atcaacatac attagttgat
    15001 ctacttcatc gctactgcag tggtgattgg ggaaatttgt gtgataacga caagaaagcc
    15061 aatgtgaaag ctattgaacg cggaggacgc attttagctc gctatgatgt ggaagatgaa
    15121 acactttatg taatcacaga ggctgatcgt agttacacta caattttatt tccttatgaa
    15181 tattaatgga gcttccattt ataccctaca tttttatgtt atcaaacgcc gtcaaatgat
    15241 ggcgtttttc tattttatat ctttcgattt tatagttgtt tcaatatgca attttaatta
    15301 tttttcctct ttgtagaaca attacaaatg aattaagcat ggaaaaacaa atatataaca
    15361 gaagaaaaac attattcggc aatgaggaga cggctgcact ttattatgca gctaaagaca
    15421 ttatctttga atatgaaatt tacaaacctt attct
//
LOCUS       DANLXS010000043        15353 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.043, whole genome shotgun sequence.
ACCESSION   DANLXS010000043 DANLXS010000000
VERSION     DANLXS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15353
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.043"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            24..914
                     /locus_tag="PXD71_000985"
     CDS             24..914
                     /locus_tag="PXD71_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6291125.1"
                     /translation="MDKLNAISLFCKVIETQSFTQAAQQAQISLPMASKLVAQLEEHL
                     NVRLLQRTTRKITPTEAGLLYYQQCLPILNELKEADASVSNINSTLQGKITMSVPMDF
                     GSRFIAPYLGKFMEDYPNLALHIEFSDRRVDVIAEGFDLVLRIGSLADSSIVAKKIAD
                     SHLVLVASPDYLARFGEPKGLEALVERDCLLYEYHPQWQFSQQGQKMQIKPQGKIYSN
                     NGYALVQMAKAGLGIINIPKFLIKHELKNGELIPIMPFLTQEPLQISLLYPNRRYLSP
                     KVRILIDFFTQLMQEQKEYI"
     gene            complement(964..1224)
                     /locus_tag="PXD71_000986"
     CDS             complement(964..1224)
                     /locus_tag="PXD71_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL6291126.1"
                     /translation="MGWIAAIIVGAIIGWIASAVMKSEGGLCKNIIIGILGSSLGRWV
                     FGDVLGIGSAYSAGDFNIVGIFFGVLGAALLIFILRKLSIIN"
     gene            complement(1304..2263)
                     /locus_tag="PXD71_000987"
     CDS             complement(1304..2263)
                     /locus_tag="PXD71_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6291127.1"
                     /translation="MKFHKTLLVTLLSSLSVAALAQDKIQNVAITAIVEHPALDSIRK
                     GVIEELAREGFVDGKNIKIDYQSAQGSTATAAQIARKFVGDKADIIIPITTPSAQPVV
                     AATRSIPIVFSGVTDPVAAKLVKSWEPSGTNVTGISDHKAMAPQVKLIQTLVPDLKAV
                     GYVYSAGEVNSAIVLEELKQEAQKQGFTIVPVAVQRSADIGTAARSLNGKVQAIYISE
                     DNAVVSAYEALHKAALEAKIPVIAADRDTVQRGAVAAYAVNQYDIGVATGKVAARVLK
                     GEKAGTIPIQEVSKLELSINTKTAKELGITIPEVLIKEAKQTF"
     gene            complement(2460..2867)
                     /locus_tag="PXD71_000988"
     CDS             complement(2460..2867)
                     /locus_tag="PXD71_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDL6291128.1"
                     /translation="MKPLRPYLYNAYYNWIIDSDNTPYLLVNAEFPDVDVPVEFVKEG
                     KIILNISPRSIGQYIVADEYISFSARFQGMLRDIYIPFGAMVAIYAQETGDGIMFQEE
                     AYYTEEAYQARVRAVEQPKKIKAKKTSTLKLVK"
     gene            complement(2867..3511)
                     /gene="sspA"
                     /locus_tag="PXD71_000989"
     CDS             complement(2867..3511)
                     /gene="sspA"
                     /locus_tag="PXD71_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDL6291129.1"
                     /translation="MTSSINKRSVMTLFSDKNDIYSHQVRIVLAEKGVPYEIENVMLG
                     SISEDLLELNPYGNIPTLVDRELVLFNPRIIMEYLDERFPHPPLMPVYPVSRGQCRLN
                     MYRIEQDWYSQIDIVNKDPESAEGKKALAQLKEDVLALAPIFAAKPYFMSDDFSLVDC
                     YIAPLLWRMQILGVKFSGATAKPINAYMTRVFQRDSFIQSVGGEAPKHLMDDKD"
     gene            3685..4182
                     /gene="purE"
                     /locus_tag="PXD71_000990"
     CDS             3685..4182
                     /gene="purE"
                     /locus_tag="PXD71_000990"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL6291130.1"
                     /translation="MPNKAEIAIVMGSKSDWATMSEATQILDQFGLSYHVEVVSAHRT
                     PDKLFSFAETAETNGYKVIIAGAGGAAHLPGMIAAKTIVPVLGVPVKSSMLSGVDSLY
                     SIVQMPKGIPVGTLAIGPAGAANAGLLAAQILAAFNPDIAEKLRIFRQTQTQMVLDNP
                     DPRVE"
     gene            4197..5162
                     /locus_tag="PXD71_000991"
     CDS             4197..5162
                     /locus_tag="PXD71_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL6291131.1"
                     /translation="MSKKLLILHTGGTISMKEGEDGKVSPSNENPLLAALERLNHPAK
                     LAQESLFNIPSPHINIEHWQQLKMRIEKAVNEENVDGVVITHGTDTLEETSYFLDLAL
                     SVNIPVVITGAMRSSNELGADGLINLQSAILVALNEESRDKGVLVVMNDEIHNAKFVT
                     KTHTTNVATFQTPTFGPCGLVTKESVIFFQKLTTYERYPIQQVTKANVQLLKAYAGMD
                     SFLLEQLAKSKCDGVVIEALGAGNLPPSCLAGIEALVNANIPIVIVSRAFNGITQDVY
                     DYVGGGKQLKQKGIIFTKGLSGQKARIKLMVLLSQTLDKPLSDYF"
     gene            5181..6269
                     /gene="purK"
                     /locus_tag="PXD71_000992"
     CDS             5181..6269
                     /gene="purK"
                     /locus_tag="PXD71_000992"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL6291132.1"
                     /translation="MQKSAIYPPVYVLGNGQLGRMLRYAGAPLDIEVKPLAFDAPVFD
                     IEPNSIITAEIERWADTPLTQLLGNHPNFVNLNVFGKLADRFTQKSLLDELHLATSKW
                     QLLENAEHWQDIFQHIGEKVVVKRRTGGYDGRGQWIVTQDSIHQITPELFGEVIAEKF
                     IPFEGEISIVGARFRDGSTRFYPISHNLQQNGILRYSVSDVNLPNQALYQQQAEQMLA
                     TVMQKLEYVGVMAMECFVVGDKLLINELAPRVHNSGHWTQLGCAISQFELHLRALLDL
                     PTPALDQIAPSVMVNLIGIEHNKQWLSLPFSQLHWYGKEVRAGRKVGHINLTHTDKNG
                     LIELLEQLRPSLTEDFHSGLDWAINKLK"
     gene            complement(6490..6900)
                     /locus_tag="PXD71_000993"
     CDS             complement(6490..6900)
                     /locus_tag="PXD71_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291133.1"
                     /translation="MISRSLFSKLILFAKIVIFSQLVFMVVIWIAHHIMREYSNPFER
                     FGTLFNNVFLTFIIPMMVSSAVIVYKGYTQKNEDKVFWIYMGFFLIWILGFIYFEPAV
                     YDKSLKISGAGFGAMLSIIICSKLLPKETNKEQL"
     gene            complement(6903..8921)
                     /gene="uvrB"
                     /locus_tag="PXD71_000994"
     CDS             complement(6903..8921)
                     /gene="uvrB"
                     /locus_tag="PXD71_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDL6291134.1"
                     /translation="MSKQGKPFILHSPFKPSGDQPSAIAKLTEGLNDGLAHQTLLGVT
                     GSGKTFTIANVIAKLNRPAMVLAPNKTLAAQLYAEMKAFFPENAVEYFVSYYDYYQPE
                     AYVPASDTFIEKDASINEQIEQMRLSATKSFLERRDTIVVASVSAIYGLGDVDAYMQM
                     MLHLQVGAMIGQREILSRLAELQYTRNDQAFQRSTFRVRGDVIDIYPAESDEVALRVE
                     LFDDEIENLSLFDPLTGHSLGKVPRYTIYPKTHYVTPRERILEAIEQIKVELADRRTY
                     FIKENKLLEEQRIAQRTQFDIEMMNELGYCSGIENYSRYLSGRKEGEAPPTLFDYMPA
                     DGILVIDESHVTVPQIGGMYRGDRARKETLVQYGFRLPSALDNRPLRFEEFERLSPQT
                     IYVSATPGAYELEKNPDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIHKRVAVDER
                     VLVTTLTKKMAEDLTDYLDEHGVRVRYLHSDIDTVERVEIIHDLRMGMFDVLVGINLL
                     REGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITNSMQKA
                     ITETERRREKQMKYNEEHGITPQALNKKVGELLDIGQSDKPKRGKQAVKKAENSATDY
                     KPKSRKELEKELKTLEQQMRDFAKDLEFEKAAAVRDKLQHLKAYLLEV"
     gene            complement(9160..9396)
                     /locus_tag="PXD71_000995"
     CDS             complement(9160..9396)
                     /locus_tag="PXD71_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL6291135.1"
                     /translation="MSRTTSVTLAEPLSQFVNQMVESGRYGSTNEVISAALKILEAQE
                     QQLIQLRALIDEGLESGVSEETVQSIISKAKRNV"
     gene            9559..10404
                     /locus_tag="PXD71_000996"
     CDS             9559..10404
                     /locus_tag="PXD71_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HDL6291136.1"
                     /translation="MFLLKGNFYAFNQTCFCTFALSGFFVATQAAALDLTKETEAYKQ
                     FVVEQIDQLVTDTEKFVGYLNKGDVQKAKKIYPLARMYFERSEPIAESFGDLDPRIDA
                     RLADLAEEGKTEKDWSGFHKIEKVLWQKNTTKGTKATAEQLLKDVKELRAKIPTAEVT
                     PEPMITGAVDLLNEVSTTKVTGEEEIFSKTDLYDFKANIEGAEKIYEIFKTQLEQIDA
                     KLSTEIGSRFAAVNALLAKHNKSKTGYDYVPYNKLSKADIKALAEAVNQLGEPLAQLG
                     VLLNK"
     gene            10415..11611
                     /gene="efeB"
                     /locus_tag="PXD71_000997"
     CDS             10415..11611
                     /gene="efeB"
                     /locus_tag="PXD71_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDL6291137.1"
                     /translation="MMAETTSRRDFLKHTALVGAGLLSAPAFALESRQISEKSQNQYD
                     FYGKHQAGIVSPAQKHIYFLVLDLDTTDIAKVKTLFQNWTEYSRNLTLGKNVKPYSAN
                     AYVPPADTGEADSLSAQNLTLTFGVSANFFTKLGIEKHKPKELNDLPHFPRDQLQAEY
                     TGGDICIQACADDPQVAFHAVRNLVRVARGEIKMKWSQMGFNSFANQDTPRNLFGFKD
                     GTANAESLKDQENTVWVQQEGWLKGGSYLAVRRVKMFLETWDRTHLQSQEETFGRHRD
                     SGAGIGHQHEFDKLDLAKKDEKGNAVIPEVSHAHLANKTGLKILRRSFSYSSGIDDKG
                     QFDSGLLFVSFQQSPEQFIKIQNALGNVDKMNEYITHIGSGLFACFAGVKDENDYLGK
                     ALFDLI"
     gene            11622..12241
                     /locus_tag="PXD71_000998"
                     /pseudo
     CDS             11622..12241
                     /locus_tag="PXD71_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+/Pb2+ permease"
     gene            complement(12277..13095)
                     /locus_tag="PXD71_000999"
     CDS             complement(12277..13095)
                     /locus_tag="PXD71_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL6291138.1"
                     /translation="MKYDLHAHSTASDGVLSPTELVQRAVEQGVTMLALTDHDTISGI
                     QEAKNFAQNQPLELISGVEISILWQEKSIHLAALNIDENNEELVKFLDNQAQLREERA
                     VEIGEKLAKVGIANAYEGAKALATGEVTRAHYGRFLYEQGYVRNIEHAFKRYLGMGKP
                     AYVKPRWSSLEDAVNVTHTSGGVISIAHPLRYKMTARWIRRLIADFKQAGGDGIEVSG
                     CGQTPDQRQLLARWAKEFDLYGSVGSDFHYPTGWIELGKSLELPQECKPIWTLF"
     gene            complement(13107..13706)
                     /locus_tag="PXD71_001000"
     CDS             complement(13107..13706)
                     /locus_tag="PXD71_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDL6291139.1"
                     /translation="MTMDNLPLEQHPFDPILPEQATVVMVGTFPPTSEKRCMEFHYPN
                     FQNDMWRIMGNIFYADPNYFRVGEEKRFDPIRIEAFLREKGVALCSSAKTAIRLKGNA
                     SDKDLKIVDPIDMDELLLKLPKLKWLFTTGGLATDTLLGLLPEKHKAPKTNEWVVYPY
                     SATRELYLYRLPSTSRAYPLSLANKTEAYRQFFRTAGLI"
     gene            complement(13703..15250)
                     /gene="sbcB"
                     /locus_tag="PXD71_001001"
     CDS             complement(13703..15250)
                     /gene="sbcB"
                     /locus_tag="PXD71_001001"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDL6291140.1"
                     /translation="MSDKFSFFFYDYESFGVSPALDRPAQFAGIRTDADFNIIGEPVM
                     FFCKQTPDYLPSPEAVMVTGITPQQCNAEGVSEPEFAARIHAEFSRANTCTIGYNNIR
                     YDDEMTRYTFFRNFYDPYEYSWKNGNSRWDLLDLVRACYALRPDGIEWPTDETGMPSF
                     KLENLTKANGIAHENAHDAMSDVYATIAMAKLIKQKQPKLFDFFFNLRSKKEVEKLID
                     TGEMTPLVHVSGMLGNYRGNTAWVVPLAWHSTNQNAVIVCDLSGDMNGLLTENAEVLR
                     QRLYTKRDELAENELPVPLKLVHINKCPILAPAKTLLPENAERLGIDRALCLENLKTL
                     KAQKSLIREKVIEIFNEERTFEPSTNVETTLYDGFFSPADKNNMAILRTLPPEDLANH
                     GLKFEDPRVEALLFHYRARHYPQTLSRAEQIKWQKHCNQQIEAKAAQFAQSIDDLFQQ
                     HHDNPEKVKLLENLTAYAEQISQQQAVIYRQNVAKDEKLLSELNRVAEQPLDKTEKLK
                     MLKELIK"
BASE COUNT         4571 a         3333 c         3048 g         4401 t
ORIGIN      
        1 ttacaatata aagaagagag aaaatggata aactaaacgc aatcagttta ttttgtaaag
       61 taattgaaac ccaaagtttc actcaagcag cacaacaagc tcaaatttcg ttacccatgg
      121 caagtaaatt ggtggctcag ttagaagaac atctcaatgt gcgtttacta caacgaacca
      181 ctcgaaaaat tacgccaact gaggcagggt tgctctacta tcagcaatgc ttgcctattt
      241 taaatgaact aaaagaggcc gatgccagcg taagtaatat taactcaacg ctacaaggga
      301 aaataacaat gtctgtacca atggattttg gctctcgctt cattgcccct taccttggga
      361 aatttatgga agattaccct aatttagccc ttcatattga atttagtgac cgtcgggtag
      421 atgtgattgc agaaggcttt gatttggtgc tgcgtattgg tagccttgca gatagttcca
      481 ttgtagcgaa aaaaattgca gattcccatt tggttttagt tgcttctcca gattatttag
      541 ctcgatttgg cgaacctaaa ggcttagaag cattagtgga acgagactgc ctactctatg
      601 aataccatcc tcaatggcaa ttttctcaac aaggacaaaa aatgcaaatt aaaccacaag
      661 gtaaaattta cagtaacaat ggttatgcgt tagtacaaat ggcaaaagct ggtttgggca
      721 tcattaacat tccaaagttt ttaataaaac acgagctaaa aaacggagag ttgattccta
      781 ttatgccttt tttgacacag gaacctttac aaatcagctt actctatcca aaccgtcgtt
      841 atctctctcc taaagtgcga atcctaattg attttttcac acaactgatg caagagcaaa
      901 aagagtatat ttaacaaaaa agaagcagtt ataaaacaac tgcttccttt ctgaaatacg
      961 aaattagtta attatgctta attttctcaa aatgaaaatc aaaagagctg cccctaatac
     1021 accaaaaaag ataccgacta tattaaagtc tccggcacta taagcggaac caatacctaa
     1081 tacatcacca aatacccagc gtccaagtga cgaacccaaa ataccaataa taatattttt
     1141 gcaaagtccg ccttcacttt tcattacagc cgaagctatc caaccaatta tagcgccaac
     1201 aataattgca gcaatccatc ccataatatt ctccttagct gagttgaaat gaatttttac
     1261 tctacaagat tatgacaaat aaaacaaatt tttatttttt acactaaaaa gtttgtttag
     1321 cttctttgat taatacctct ggtatagtaa tgcccaattc ttttgccgtt ttagtattaa
     1381 ttgaaagctc taatttgctg acttcttgga ttggaattgt acctgctttt tcccctttta
     1441 atacacgagc agcaacttta ccggttgcca caccaatatc atattgatta accgcataag
     1501 ctgccactgc accacgttgt actgtatcac ggtcagcagc aatcaccgga atttttgctt
     1561 ctaaagccgc tttatgtaag gcttcataag ccgaaacaac tgcattatct tcagaaatat
     1621 aaatagcctg cacttttccg tttaggctgc gagcagcagt gccaatatcg gcactgcgtt
     1681 gaacagcaac cggcactata gtaaagccct gtttttgagc ctcttgtttt aactcttcta
     1741 acacaatcgc agaattaact tcacctgcac tatacacata accaacagct ttcaaatccg
     1801 gtactaatgt ttgaattaat ttcacttgtg gagccatcgc tttgtggtct gaaatccccg
     1861 tcacgttagt gccacttggc tcccacgatt ttactaattt tgcagcaacc gggtctgtca
     1921 cgcctgaaaa cacaattggg atagaacgtg ttgcagcaac aacaggttgt gccgaaggtg
     1981 tggtgatcgg gataataata tctgctttat ccccaacaaa tttacgcgca atttgtgctg
     2041 ctgttgcagt actaccctgt gcagactgat agtcgatttt aatgttttta ccatcaacaa
     2101 acccttctcg ggctaattct tcaatcacac ctttacgaat tgaatctaat gcagggtgtt
     2161 ccacaatcgc tgtaattgct acattttgaa ttttatcttg agctaatgcc gctacagaaa
     2221 gtgaacttaa caaagtcact aataaggttt tatggaattt cattttctct ccttaagatc
     2281 attactgtac tatcacaaca gtacaaacac aaaacaaaga taagcaattt ttagtcggag
     2341 tgcaagtgga atttgattat ttccgtcaca aaacaaacaa gcggttaaat tttgactgat
     2401 atttgcaaat aatcagaaaa atttaaccgc ttgtaatgtg ttttgatgaa atagatctct
     2461 tatttcacca actttaaggt agatgttttc ttcgccttga tttttttagg ctgctctact
     2521 gctctaactc gagcttgata agcctcttca gtgtagtagg cttcttcttg gaacataata
     2581 ccatcaccgg tttcttgcgc ataaatcgcc accattgccc caaaaggaat ataaatatct
     2641 cttaacattc cctgaaaacg agcgctaaag ctaatgtatt catcagccac aatatactgc
     2701 ccgatggaac gaggcgaaat atttaaaata atcttccctt ctttgacaaa ctctaccggc
     2761 acatctacgt ctggaaattc tgcattcact aataagtaag gcgtgttatc gctatcaata
     2821 atccagttat agtaggcatt ataaagataa ggtcttaatg gtttcattag tctttatcgt
     2881 ccattaaatg ttttggcgct tcgccgccaa cagactggat aaaactatca cgttggaata
     2941 cacgggtcat atacgcatta attggttttg ccgttgcacc gctgaattta acgcctaaga
     3001 tttgcatacg ccataataac ggtgcaatat agcaatcaac caagctgaaa tcatcgctca
     3061 taaaataagg tttagcggca aaaataggcg ctaatgccaa tacatcttct ttaagctgtg
     3121 ctaacgcttt cttgccttcc gctgattcag gatctttatt cacaatatca atttgcgaat
     3181 accaatcttg ttcaatacga tacatattta aacggcactg accacgagaa acagggtaaa
     3241 ccggcattaa tggtgggtga gggaaacgct catctaaata ttccataata atacgagggt
     3301 taaacagcac taattcacga tcaaccaatg taggaatatt accgtaagga ttcaattcta
     3361 ataaatcttc agaaatactg cctagcataa cgttctctat ttcataaggc accccttttt
     3421 ctgctaacac aatacgcact tggtggctgt aaatatcatt tttatccgaa aaaagagtca
     3481 ttaccgaacg tttgtttata ctactcgtca tttatatctc cgatgattaa atttataaaa
     3541 aataaaatgg ttgtcgatta tatcatattt taaccagcag ataacagccc taaaaattgc
     3601 ttttcacttc ttaaatagct acgcaaacgt ttgctttgta tgctatgatt acactgtttt
     3661 acattagtat cataaggatt taaaatgcca aataaagctg aaattgcgat cgtaatggga
     3721 tctaagagtg attgggctac aatgtctgaa gcaacccaaa tattagacca gttcggtttg
     3781 agctatcacg ttgaagtcgt ctcagcacac cgcacaccgg acaaactctt ttcttttgct
     3841 gaaactgcag agactaacgg ctataaagtg attattgccg gagccggcgg tgcagctcac
     3901 ttgcccggaa tgatcgctgc taaaaccatt gtgccggtgt taggtgtgcc ggtaaaaagc
     3961 tcaatgttaa gtggtgtaga tagcctctat tcgattgtgc aaatgccgaa aggtattccg
     4021 gtcggtacgc tcgcaatcgg gccggcaggt gcagcaaatg caggcttatt agccgctcaa
     4081 attttagcag cctttaaccc tgatattgca gagaaactgc gtattttccg tcaaacccaa
     4141 actcagatgg tattagataa tcctgatcct cgtgttgaat aactattgta taaaagatgt
     4201 ctaaaaaatt attaatcctc cacactggtg gtactatttc aatgaaagaa ggggaagacg
     4261 gtaaggtctc cccttccaat gaaaatcctc tcttggctgc cttagaacgt ttaaaccacc
     4321 cagcaaaatt ggcgcaagaa tcgttattta atattccctc tccgcatatc aatatcgagc
     4381 attggcaaca gttaaaaatg cggattgaaa aggcagtgaa tgaggagaat gtcgatggcg
     4441 tagtaatcac ccacggcaca gataccttgg aagaaacctc ctattttctg gatttagccc
     4501 tcagcgtaaa tataccggta gtaattaccg gagcaatgcg ttccagtaac gaactgggtg
     4561 ctgatggctt aatcaatcta caaagtgcga ttttagtggc gttaaatgaa gaaagccgag
     4621 ataaaggcgt gttagtggtg atgaatgatg aaatccacaa tgctaaattt gtgaccaaaa
     4681 cccacaccac taatgtggcg actttccaaa ccccaacctt tgggccttgt ggcttagtaa
     4741 cgaaagaatc tgtcattttt ttccaaaaat tgaccactta cgaacgttat ccaattcaac
     4801 aagtaactaa agctaacgtg caactactga aagcatacgc cggaatggat agttttctgc
     4861 tggaacaatt agcgaaaagc aaatgcgatg gtgttgtgat tgaggcattg ggtgcaggta
     4921 atttaccccc tagttgtctg gctggtattg aggctttagt gaatgccaat attccaattg
     4981 taattgtttc tcgggcattc aatggtatta cccaagatgt ctatgattat gtaggcggtg
     5041 gaaaacagtt aaagcaaaaa ggcattattt ttaccaaagg cttaagtggg caaaaagctc
     5101 gcattaaact tatggtctta ctcagccaaa ccttagacaa accactatca gactatttct
     5161 aacccattga gaataccacg atgcaaaaaa gtgcaatcta cccacctgtg tatgtattag
     5221 gcaacggcca gctcgggaga atgctacgct acgccggagc accacttgat attgaagtca
     5281 aaccgctggc tttcgatgcc cctgtgtttg atattgagcc aaacagcatt attaccgcag
     5341 aaatcgaacg ttgggcagat acgcctttaa cgcaattact cggtaaccac ccgaattttg
     5401 tgaacctgaa tgtttttggg aagttagccg atcgttttac gcaaaaatca ctacttgatg
     5461 aactccatct tgcgacttca aaatggcaac tgctagaaaa tgccgaacat tggcaagata
     5521 tttttcagca tattggcgaa aaagtagtgg ttaaacgccg aaccggagga tatgacggac
     5581 gtgggcaatg gattgtaacg caagactcga ttcatcaaat tacacctgaa ttatttggcg
     5641 aagtgattgc cgaaaaattt atcccgtttg agggggaaat ttccattgtt ggggcgagat
     5701 tccgtgacgg ctcaacccgt ttctacccta tttctcacaa tttacaacaa aacggcattc
     5761 tacgttactc cgtctccgat gtgaatttac cgaaccaagc cctctaccag cagcaagccg
     5821 aacagatgct cgctacggta atgcaaaaat tagagtatgt cggcgtaatg gcaatggaat
     5881 gctttgttgt gggcgacaag ctactcatca acgagcttgc ccctcgagtg cataacagcg
     5941 gacactggac acaactcggc tgtgccatta gccaatttga gttacattta cgggcattat
     6001 tagatctacc tacgccagca ctcgaccaaa ttgccccaag cgtgatggtt aatttaatag
     6061 gcattgaaca taacaagcag tggctaagcc tcccatttag ccaactacat tggtatggca
     6121 aagaagtccg agctggtaga aaagttgggc atatcaacct cactcacacc gataaaaacg
     6181 gcttaattga actactggaa caactcagac cgagcctaac ggaagatttc cattcgggat
     6241 tagattgggc aatcaataag ctcaagtaag cggtcggatt ttgtaacttt tttgcaaatc
     6301 tgtttacccg atggtgcgag cttcctcgct cgtaccaaac aagcggtcga cttagtgcta
     6361 aaatttgcaa aataattcaa cataatggct acttccggtc atcatcttga tctgaaaacc
     6421 cctcagggtg gatatggcta tcaaaaagca ttttcttcct ccgagaaaac agatttcttc
     6481 acaagaatct tagagctgct ctttatttgt ttccttaggc aataacttcg agcaaatgat
     6541 aatagagagc atagccccaa atcctgcacc tgaaatcttc agtgatttat cataaactgc
     6601 cggctcaaaa taaataaatc ccaaaatcca aattaaaaag aaccccatat aaatccaaaa
     6661 cactttatct tcatttttct gggtataacc tttataaaca ataaccgcac tacttaccat
     6721 catcggaata ataaaagtca aaaacacatt attgaacagg gtaccaaatc gttcaaacgg
     6781 attactgtac tcccgcataa tatggtgggc aatccagata accaccataa aaacaagttg
     6841 agagaaaata acaattttgg caaataagat taattttgaa aataatgaac gagaaatcat
     6901 atttaaacct ccaacaaata cgcctttaaa tgctgcaact tatcccttac cgcagccgct
     6961 ttttcaaatt ccagatcttt cgcaaaatca cgcatttgtt gttccagcgt tttaagctct
     7021 ttttccagtt ctttacggga tttcggcttg tagtcggttg cagaattttc ggcttttttg
     7081 accgcttgtt tgccccgttt tggcttgtcg gattgtccga tgtcgagcaa ttcacccact
     7141 tttttgttca acgcttgtgg cgtgatgccg tgttcttcgt tgtatttcat ctgcttttca
     7201 cggcggcgtt cggtctcagt aattgctttt tgcattgagt tggtaatgcg gtcgccgtat
     7261 aaaatcgctt ttccgttgag gtttcgggcg gcacgtccga tggtctgaat taacgagcgt
     7321 tcggaacgta aaaatccctc tttatccgca tcaagaatgg caaccagcga cacttcgggc
     7381 atatctaagc cctcacgcaa taagttgatc cccaccagca catcaaacat tcccattcgt
     7441 aaatcgtgga taatttcgac ccgttccacc gtgtcaatgt cgctatgtag ataacgaact
     7501 cgtacgccat gttcgtccaa ataatcggtg agatcttccg ccattttttt ggtgagtgtt
     7561 gtcaccagca cccgttcgtc caccgccact cgtttgtgaa tttcagacag taaatcgtcc
     7621 acctgcgtag caacggggcg aacttcgata atcggatcga gcaagccggt tggacgaact
     7681 acttgatcca ccacatcggg atttttctcc aattcatacg cccccggtgt ggcagaaacg
     7741 taaatcgtct gtggtgaaag acgttcaaac tcttcaaatc tcaacgggcg gttatccagt
     7801 gcagacggca gacggaaacc gtattgcacc aatgtttctt ttctcgctcg gtcgcctcga
     7861 tacattccgc caatttgtgg cacagtaacg tggctttcat caatcaccaa aataccgtcc
     7921 gccggcatat aatcaaacag ggtcggaggg gcttcgccct cttttctgcc cgagagatag
     7981 cgtgagtagt tttcaatgcc cgaacagtag cccagctcgt tcatcatctc aatatcaaac
     8041 tgcgtacgtt gggcgatccg ctgctcttcc agcaatttat tctctttgat gaaataggtg
     8101 cgacggtcgg caagctcgac cttaatttgc tcgattgcct ccaaaatccg ttcccgaggg
     8161 gtcacatagt gagttttcgg gtaaatggta tagcgaggca ccttgcccaa gctatgccct
     8221 gtaagcggat caaacagcga cagattttcg atttcatcgt caaacagctc tacccttaac
     8281 gctacttcat cgctttcggc agggtaaata tcaatcacat cgccccgcac tcggaaagtc
     8341 gaacgttgga acgcctgatc gttgcgagta tattgcaatt cggcaaggcg agagagaatt
     8401 tcacgctgcc caatcatcgc ccccacttgc aaatgcaaca tcatctgcat ataagcgtcc
     8461 acatcaccca agccgtaaat ggcagaaacg gacgccacta caatcgtatc tcggcgttcc
     8521 aaaaaggatt tggttgcgga aagtcgcatt tgctcgatct gctcattgat agaggcatct
     8581 ttctcgataa aggtatcgct tgccggcaca taggcttcgg gctggtagta atcatagtaa
     8641 gagacgaaat actccaccgc attttccgga aagaacgctt tcatctcggc ataaagctgg
     8701 gcggcaaggg ttttattcgg ggcaagcacc atcgccgggc gattgagctt ggcaatcaca
     8761 ttagcaatgg taaaggtttt ccccgagccg gttacgccaa gtaaggtttg atgtgccaag
     8821 ccgtcattta agccttcggt caatttggca atcgcacttg gttggtcgcc ggagggcttg
     8881 aaaggagaat ggaggataaa gggtttgcct tgcttgctca tagaaaatcc tgtcttaaaa
     8941 aatcggttta ttttagcaca agcaagcggt cgtttttatc gggatttttg caaatttatt
     9001 gtaactgctt atattgatcc attcttggat ctaacaccct aacaataaag ataccttttg
     9061 ggattggttt ataataaata gaatgtgagc ggtggtaatg acagcgtaac aaagggcgaa
     9121 taaagtaacg ctaaatctcg ctctgcccta acagaaagtt tatacattac gttttgcctt
     9181 gctgataata gattgaacgg tttcctcaga aacaccgctt tctaagcctt catcaattaa
     9241 ggctcttagc tgaattagtt gctgttcttg tgcttccaat atttttaatg cggcactgat
     9301 gacctcgttg gtcgaaccat aacgcccact ttcaaccatt tggttcacaa attggcttaa
     9361 tggctcagct aaagttacgc tggttgttct actcattact attcctatct tattgattat
     9421 tgagcagatt gtaatggctc taatttagat aagcaagcgg tctattttta gcaataattt
     9481 gcaaaacaaa atgtgattta catcacaaaa tcagcattat tttttgcgaa taattcgcaa
     9541 taaggatatt atgtcgcaat atttttatta aaaggaaatt tctatgcgtt taaccaaact
     9601 tgcttttgca cttttgcact ttctggcttt tttgtggcta ctcaagcggc agctttagac
     9661 ttaactaagg aaaccgaggc ttataaacaa tttgtggtag aacagattga ccaactggtt
     9721 accgatacgg aaaaatttgt cggctactta aacaaaggcg atgtgcaaaa agcgaagaaa
     9781 atttacccgc ttgcaagaat gtattttgaa cgttctgaac cgattgcaga aagttttggc
     9841 gatttagacc ctcgtattga tgcccgtctg gcagatttag ccgaagaagg taaaaccgag
     9901 aaagattggt caggtttcca taaaatcgag aaagtattgt ggcagaaaaa caccactaaa
     9961 ggcacaaaag caacggcaga acaattatta aaagatgtaa aagagctgcg tgcgaaaatt
    10021 cccactgcag aagttacccc tgagccgatg attaccggtg cggtagattt attaaacgaa
    10081 gtttctacta ctaaagtaac cggtgaagaa gaaattttct ctaaaaccga cctttacgat
    10141 tttaaagcca atattgaagg tgcagaaaaa atctatgaga tttttaaaac gcaattagag
    10201 caaattgatg ccaaactctc aacagaaatc ggctctcgtt ttgcggctgt caatgcgctg
    10261 ttagcaaaac acaataaatc taaaaccggt tatgattatg tgccttataa caaactgtca
    10321 aaagcggata ttaaagcgtt agcagaggct gtaaaccaat taggcgagcc gttagctcaa
    10381 cttggcgtac ttttaaataa atagtggagc aaaaatgatg gcagaaacca cttctcgccg
    10441 tgatttttta aaacataccg cattagtagg agcaggctta ttgtctgctc ctgctttcgc
    10501 tttggaatca cgacaaatat ctgaaaaatc tcaaaatcaa tacgactttt acggcaaaca
    10561 tcaagctggt attgttagcc ctgcacaaaa acatatctac tttttagtgc tggatttaga
    10621 caccactgac attgccaaag taaaaacatt gtttcaaaac tggacggaat atagccgtaa
    10681 cctcacgctt ggcaaaaatg tgaaacctta tagtgctaat gcttatgttc caccggcgga
    10741 taccggtgag gcagatagcc tttcggcaca gaatctcact cttacttttg gcgtcagtgc
    10801 caatttcttt acaaagctcg gcattgaaaa acataagccg aaagaactga atgatttacc
    10861 gcatttccct cgtgatcaac tacaagcaga atacaccggt ggcgatattt gcattcaagc
    10921 ctgtgcagac gatccgcaag tggctttcca cgctgtgcgt aatttggtgc gtgttgcccg
    10981 tggtgaaatc aaaatgaaat ggtcgcaaat ggggtttaac tcctttgcca atcaagacac
    11041 acctcgtaac ttattcggtt ttaaagacgg cactgccaac gcagaaagtt taaaagatca
    11101 ggaaaatacg gtatgggtgc aacaagaggg ctggctcaaa ggcggttctt atttagccgt
    11161 tcgccgtgtt aaaatgttct tggaaacttg ggatcgcacc catctccaat cccaagaaga
    11221 aaccttcggt cgtcaccgtg attccggagc cggaatcggg catcagcacg aatttgataa
    11281 attagattta gccaaaaaag acgaaaaagg caatgcagta attccggaag tttcacacgc
    11341 tcatcttgcc aataaaacgg ggctgaaaat cctacgccgt tcattctcct attcaagcgg
    11401 tattgatgat aaagggcaat ttgattcagg gttgttgttt gtctctttcc aacaaagtcc
    11461 ggagcaattt atcaaaatcc agaacgctct cggcaatgtg gataaaatga atgaatacat
    11521 cactcatatc ggcagtggtt tatttgcctg ttttgccggt gtgaaagatg aaaatgacta
    11581 ccttggcaaa gcgttgtttg atttaattta gggaaaagga aatgaaatta aaagcattag
    11641 tgctaattcc gttactcttt tcgagtacag gattgcttgc caaagtgaat gtcagcccct
    11701 tatttgttca gctttccgat gcaatggcag agagtaaaaa aggggaattt gcaaaatcca
    11761 gccaaaattt gaccgcttta cagcacgatt ttgagcagat tagccagcac gattccctcg
    11821 ccggagttga agtacagacc gctctgaaaa aagcgattga aacacccaat gctgaaaact
    11881 tagagagcct ttctaaaaac tctatgcgtt tgaaaaagaa caaaacccta gcggttatgc
    11941 tgaaaaacat caggattttg tggcaaaaat gacgccgctt tatgcagaac ttgctcaaac
    12001 cgtgccaact caagaaatcg ccaaaatcaa atgggcggct tatcagtttg gtaagaattg
    12061 ggtaaaacag gaaaaagcag tacgggaaat cagtcttccc cattatggca aatttgagcg
    12121 tattcttggt ttaatgcgaa ttaatctgaa cgctgaaaag ccggatatgg caaaaatcag
    12181 tgaactggtt tccgaactag gtgtggtaat ggcagacttt aagcaagtta aagtgaaata
    12241 gtaaaaaatc ggggcaaatt atttgccccg ataacactaa aataacgtcc aaatcggttt
    12301 acattcttgg ggtaattcca aacttttacc gagctcaatc cagccagttg ggtagtggaa
    12361 atcagaaccg accgagccat ataaatcaaa ctcttttgcc caacgggcaa gtaattgccg
    12421 ttgatcaggt gtttgtccgc agcctgatac ttcaatgcca tcgcctccgg cttgtttgaa
    12481 atctgcgatt aaacggcgaa tccaacgagc agtcatttta tagcgtagcg ggtgagcaat
    12541 cgaaatcaca ccgcctgagg tgtgggtaac attcaccgcg tcttctaaac tactccatct
    12601 cggtttcaca taggcaggtt tccccattcc cagataacgc ttaaaggcgt gttcaatatt
    12661 gcggacatag ccttgttcat acaaaaaacg cccataatgt gcccgagtta cttcccctgt
    12721 tgccaacgct tttgccccct cataggcatt tgcaatcccg acttttgcta atttttcacc
    12781 aatttcgacc gctctctctt cccttaattg ggcttggtta tccaaaaact tcaccagctc
    12841 ctcgttgttt tcatcaatat tcaacgccgc aagatgaata cttttttctt gccataaaat
    12901 cgaaatttcc accccagaga taagctcaag cggttgattt tgggcaaaat ttttcgcttc
    12961 ttgaatgccc gaaatcgtat cgtgatcagt taaagctagc attgttaccc cttgctctac
    13021 cgcacgttgt accaattcag tgggagaaag tacgccatca gaggcggtac tgtgggcgtg
    13081 taaatcatat ttcattttat ttatccttaa attaagccgg cagtcctgaa aaattggcga
    13141 taggcttcgg ttttgttggc taagctgagt ggataagccc tcgacgtcga aggcaggcga
    13201 tataaataaa gttctcttgt ggcggaataa ggatagacca cccattcgtt ggttttcgga
    13261 gctttgtgtt tttccggcaa taaaccgagc aacgtatcgg ttgccaaacc acctgtggta
    13321 aatagccatt tcagtttggg taatttgagc aacagttcgt ccatatcgat aggatcaaca
    13381 atttttaaat ctttatctga agcgtttcct tttaaacgaa tcgctgtttt tgccgaagaa
    13441 cagagcgcaa cgcccttttc tcgcaaaaac gcctcaatcc gaatcggatc aaagcgtttt
    13501 tcttcaccaa ctcgaaaata gttagggtca gcataaaaaa tattgcccat aattcgccac
    13561 atatcatttt gaaaattcgg gtaatgaaat tccatacaac gcttttccga tgtaggtgga
    13621 aaagtgccga ccattaccac cgttgcttgt tcaggtaaaa tagggtcaaa cgggtgctgt
    13681 tctagcggta aattatccat tgtcatttaa tcaactcttt taacatcttg agcttttcgg
    13741 ttttatctag gggctgttcc gctactctgt taagctcgct cagcaatttt tcatcttttg
    13801 caacattttg acgataaatg accgcttgtt gttgcgaaat ctgctcggcg taagcggtca
    13861 aattttctaa taattttacc ttttccgggt tgtcgtgatg ttgttggaac aagtcgtcaa
    13921 tcgattgggc gaactgcgct gcttttgcct caatttgctg gttgcaatgt ttctgccatt
    13981 tgatctgctc ggctcgactg agtgtttgtg gatagtgcct tgctcggtaa tggaatagta
    14041 acgcctctac tcgagggtct tcaaatttca agccgtggtt ggctaaatct tcaggcggta
    14101 gcgtgcgtaa aatcgccata ttatttttat ccgcaggcga gaaaaagccg tcatacaaag
    14161 tggtttctac attggtcgaa ggctcaaacg tgcgttcttc attaaaaatt tcaatcactt
    14221 tttcccgaat cagcgatttc tgtgctttta aagttttcaa attttccaaa cataaagctc
    14281 tgtctatgcc taaacgttct gcattttcag gcagtaaggt ttttgccggt gctaaaatcg
    14341 ggcatttatt aatatgcact aattttaacg gcacaggtaa ctcattttcg gctaattcat
    14401 ctcgtttggt gtataaacgc tgacgtaaaa cctctgcatt ttcagtcaat aagccattca
    14461 tatcgcccga taaatcacac acaattacgg cattttgatt agttgagtgc caagccagtg
    14521 gcacaaccca agcggtattt cctcgatagt tgcccagcat accggaaacg tgcacaagcg
    14581 gtgtcatttc accagtgtca attaacttct ctacttcttt tttactgcgt aaattaaaga
    14641 aaaaatcgaa caactttggc tgtttttgct taattaattt tgccattgcg atggtggcat
    14701 acacatcaga catcgcatcg tgtgcatttt catgggcaat gccattggct ttggtgaggt
    14761 tttccaattt aaaacttggc ataccggttt catctgttgg ccattcaata ccatcaggtc
    14821 ttaacgcata acaggcacgg actaaatcta acaagtccca acgagaattt ccgtttttcc
    14881 agctatattc gtaaggatcg taaaaattgc ggaagaaggt ataacgggtc atctcatcat
    14941 cataacgaat attgttataa ccgatagtac aagtattcgc tcggctaaat tcagcgtgaa
    15001 ttcgggctgc aaattccggc tcagatacac cttcagcgtt acattgttgc ggtgtaatcc
    15061 cggtaaccat caccgcttcg gggctgggta aataatccgg cgtttgctta caaaaaaaca
    15121 ttaccggttc accgataata ttaaaatccg catcggttcg aataccggca aattgtgccg
    15181 gacgatctaa cgccggactc acgccgaaac tttcataatc gtaaaagaaa aaagagaatt
    15241 tatcgctcat tcgttcaatt tgttagaaaa aattgggctg attgtagcac agtaaggcag
    15301 aaaggaggga atacaagcgg tcaatttccg ttagaaattt gcaaatgcta aaa
//
LOCUS       DANLXS010000044        15232 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.044, whole genome shotgun sequence.
ACCESSION   DANLXS010000044 DANLXS010000000
VERSION     DANLXS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15232
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.044"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            177..554
                     /locus_tag="PXD71_001002"
     CDS             177..554
                     /locus_tag="PXD71_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDL6291141.1"
                     /translation="MEYQFTHSIHGVVAKCSMDHEAFARWLNAEITENPSQLAPIFAE
                     IEKCRAAFPNHYECVFEGREYSLYFDYDEVMAKANNLDAAFDDEEMEEGFQFYNEESI
                     AFCGLDDFEKFLKAYQHFVQTYH"
     gene            643..1230
                     /gene="yihI"
                     /locus_tag="PXD71_001003"
     CDS             643..1230
                     /gene="yihI"
                     /locus_tag="PXD71_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDL6291142.1"
                     /translation="MSRTKKTRRITDIMPARKTDKPKQPMPKLGGGKNRKLTRYELDA
                     QAREEKRKRKHKGLPTGSRNADPAEQKKAVVKEVKDPRIGSRKKVPLMVEFVNQPEKG
                     RTIKAVPVEPIKPTLSPEQELEQLENNECLNQLLDDLEAGKTLSAEDQKFMNECLDRI
                     DELMTELGIEYEDEEGDNGDALLRQFETMDINKFR"
     gene            1244..1711
                     /locus_tag="PXD71_001004"
     CDS             1244..1711
                     /locus_tag="PXD71_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HDL6291143.1"
                     /translation="MLRIFLMVLAALILISLGGYALHLMLKVRAQKKHEQALIEQAKQ
                     AQKDRYIRILDSIEVIARAMISEQCDFSEGVLRLKPLLDVLGRKLSDYTAMWALYGVV
                     ENMPILDERKNLKRNERMKLDLEREAKEAELESDIKNECYQLLKDIEEMRKAI"
     gene            1711..3078
                     /gene="hemN"
                     /locus_tag="PXD71_001005"
     CDS             1711..3078
                     /gene="hemN"
                     /locus_tag="PXD71_001005"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL6291144.1"
                     /translation="MSEIIWDLALIQKYNHSGPRYTSYPTALEFNENYTNEDFIRAAA
                     RYPERPLSLYVHIPFCHKLCYFCGCNKVITRHRHKVEIYLDYLEREIKTRAPLFQNRL
                     VTQIHWGGGTPTYLDEDQSARLMQMLRANFDVSDDAEISIEMDPREIELSMLDHLRAI
                     GFNRLSMGIQDFDKEVQRLVNREQDEEFIFALMKRAKELGFVSTNVDLIYGLPKQNVE
                     SFMYTLKRVVELNPDRMSVFNYAHLPSRFAAQVKIKEELLPAPETKLTILQNSIEFLG
                     KNGYKFIGMDHFAKPDDELAIAQEKGILHRNFQGYTTQEECDLLGMGVSAISLLGDTY
                     AQNRKELQQYYADVETNGIALHKGIALTQDDCIRRDVIKALICNFKLDFATIEKQYGI
                     DFKRYFAEDLAFLAPLAEDELLTMNDDSIVVSPRGRLLIRNICLCFDVYSRQLARRQQ
                     FSRII"
     gene            3152..5485
                     /gene="lptD"
                     /locus_tag="PXD71_001006"
     CDS             3152..5485
                     /gene="lptD"
                     /locus_tag="PXD71_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDL6291145.1"
                     /translation="MKQRYSLLSLAVMAACYSNSAAADLKQQCLLGVPHFQGEVVEGD
                     QLRMPVTIEADSAVINQPRDATYTGDVTIKQGNRSIWADQVRVEQDGENARMAYLQGN
                     FNYQDNLIQATGSDATINLLSKDANLANANYQLIGRQGRGTAESGEFGNSKHTLKNAS
                     FTSCLPNDNSWQIEANEMIQHVDEEYAEMWHARFKVLGMPVFYSPYLQFPIGDRRRSG
                     LLIPSFERSSKNGWTYSQPFYWNIAPNMDSTITPTYYSRRGWQISPEFRYLTKIGQGL
                     VATEYMAKDRLSEYQPGNDDRKRYLFHWRHAMSFLTDWRFSVDYTKVSDRRYFSDFDS
                     DYGSSTDGYATQNFKLGYYQPNYNISISGKKFQTFDELDIGPYRVLPQIDFNYYKNDL
                     IKGGDFKFFAQAARFENDSKLMPKAWRFHAEPTLNFPLANRYGSLNLETKLYATYYQQ
                     TKGSESTEEIKKSVSRVLPQIKLDFKTVLESDKQLFSGYTQTLEPRVQYIYRPYKDQS
                     DIGSKAHRSVSLGYDSALLQNDYFSLFNDRRYSGLDRISSANLVTAGGTTRFFKEATG
                     EEVFNFSLGQTYYISPSKIDDTSDNSTKGHTTSWAAESNWKFHKNWNWHGSYQYDTRL
                     NQTALANMSLQYKPQSDKLIQLNYRYASKDYINQNLNTNKYGQSIKQVGGVLGWELTD
                     KVSVMTSYYHDLALKKPVEGQISLNYNTCCWAANVYVARRLVSTPVGSSDTINDFYYD
                     NKFGINFELRFGTNYSNGVTRMLKRGIIPYTEPYSIN"
     gene            complement(5627..8266)
                     /gene="ppc"
                     /locus_tag="PXD71_001007"
     CDS             complement(5627..8266)
                     /gene="ppc"
                     /locus_tag="PXD71_001007"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDL6291146.1"
                     /translation="MNQQYSTIQHNIHMLGEFLGETIRDAQGNYILELIESIRVLSRN
                     SRAGDENAREQLLDKLANISNDDVLPVARAFSHFLNLTNIAEQHQTVSRHHIDESLGS
                     RSLDSLFLRLKSQNIPVEKVIKTVESLMIDLVLTAHPTEVTRRSLGHKHVEINKCLNL
                     LEHDDLTEAESYKIKRRLMQLIALAWHTNEIRTQRPTPVDEAKGGMAVIENSLWKAVP
                     EFCRQLNFYLEKHFGVQYPVNLAPVRFSSWMGGDRDGNPFVTAETTRRVLRMNRWKAT
                     ELFLQDIKNLAEELSITQCTPAFRAKYGNHLEPYRVLLKVLRQKLINTEIYYAELLEG
                     KISTIDESDILTSDQQLWEPLFDCYESLQACGMRIIANGALLDCLRRINCFGLGLSRL
                     DIRQESTRHEMAIAEITRYIGLGDYAHWTEDDKQAFLVRELSSRRPLIPTNWTPSPET
                     KEVLDTCKIVAEQPEGVISAYVISMAREASDVLSVHLLLKEAGCKFTIPVAPLFETLG
                     DLDHSEKVMTDLFNIGWYRGVINNYQMIMIGYSDSAKDAGMFAAGWAQYRAQEALVNL
                     CEKYNIELTLFHGRGGTIGRGGAPAHAALLSQPPRSLKNGLRVTEQGEMIRFKLGLPA
                     VAVESLELYASAILEANIFPPPEPKQQWRDVLAKASDISCQIYRDMVRGELDFVPYFR
                     SATPEQELSRLPLGSRPAKRNPKGGVESLRAIPWIFAWMQNRLMLPAWLGAGQALQQL
                     IDEGNETLLKEMCNEWPFFSTRIGMLEMVFSKADIWLAEYYDQRLVEPKLHRLGNMLR
                     TQLQADLKTVLALAHDGQLMADLPWIAESIALRNIYTDPLNLLQVELLDRIRKQGENA
                     DPVLEQALMITITGIAAGMRNTG"
     gene            8391..9359
                     /locus_tag="PXD71_001008"
     CDS             8391..9359
                     /locus_tag="PXD71_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL6291147.1"
                     /translation="MCLKLLFGFDNLPVVYVLFTVIQVGLSINGFKGIKWLENVSCVF
                     LIGILAYMLYVVNTKFSVEIGERFANIEGTWGMPFWAATTAFLGIYSTMILNASDYSR
                     HVKKGTTPTATGVIYVLSILPVTLFMGLIGLLVTAATGNSDPIEVFATAMDNKFLTVI
                     TLLFIAFAQVTTNVLNNIVPPSYILMDSFKMKWSHATILVGILSICVMPWKLVTAESA
                     DGLSLFIKIYSAFLGPIFAVMVVDYYILRKRTLNVNDLYNKEGIFKGINWAAIIAIAI
                     GSFCSLIVVDLSWYVSLIPSGLSYYILMRVLKSSEAFRKGTVLEHQ"
     gene            complement(9445..10947)
                     /gene="lysS"
                     /locus_tag="PXD71_001009"
     CDS             complement(9445..10947)
                     /gene="lysS"
                     /locus_tag="PXD71_001009"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL6291148.1"
                     /translation="MSEVENQELDLNGEMLARREKLNKIREQGNAFPNTFRRDALAKD
                     LHAQYDAVEGETLKEQNIQVKVAGRIMLKRVMGKASFFTIQDVSGAIQLYVARDNLSD
                     SVYEEKVSLWDLGDIVGVEGALFKTKTGELTVRCSEVQLLTKSLRPLPDKHHGLTDLE
                     TRYRQRYLDLISNEESRRTFMIRSQVVSGIRKFFLERDFIEVETPMLQVIPGGAAAKP
                     FITHHNALDVDMYLRIAPELYLKRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEY
                     YQAYADYHDLMDNTEELLRQLALDILGTTDVPYGDYVFDFGKPFERITMHDAIVKYGN
                     GIQREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAE
                     ISPLARRNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEA
                     MFKDEDFIVALEHGLPPTAGEGLGIDRLVMIFANAPSIRDVILFPAMRQK"
     gene            complement(11101..12576)
                     /gene="rng"
                     /locus_tag="PXD71_001010"
     CDS             complement(11101..12576)
                     /gene="rng"
                     /locus_tag="PXD71_001010"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDL6291149.1"
                     /translation="MSSTELLVNVTPSETRVALVENGILKELHIEREAKRGIVGNIYK
                     GRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTECVDENEKKQFLVKDISELVREGQ
                     DIVVQVVKDPIGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKSLVE
                     PYCDELGGFIVRTAAEEATEESLKQDVEFLKRLWRKVLERKGKYSAKSMLYGELALAQ
                     RVLRDFIGTHINSVRIDSKLTYEQVREFLAEFMPELTDCVTLYTGQQTLFDAYGVEKA
                     IQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHTIFNTNIEAIQAIAH
                     QLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRK
                     RTRESLERILCCDCQACQGRGTVKTVETVCYEIMREIVRVHHLFKTENIHVYAAKDVA
                     EYLINEESHALLAELQAFIGKKIEIKLEPYYHQEQFDVVVM"
     repeat_region   12826..13660
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12904..12923
                     /rpt_unit_seq="cagccacgcgtaggtggctg"
     gene            complement(13852..14145)
                     /gene="cas2"
                     /locus_tag="PXD71_001011"
     CDS             complement(13852..14145)
                     /gene="cas2"
                     /locus_tag="PXD71_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDL6291150.1"
                     /translation="MLMLITYDISFDDPEGQARLRRIAKHCLDYGVRAQYSVFECDVT
                     PAQWVTLKNKLLATYNPDCDSLRFYHLGSKWRNKVEHHGAKPSVDIFKDTLIL"
     gene            complement(14212..15225)
                     /gene="cas1c"
                     /locus_tag="PXD71_001012"
     CDS             complement(14212..15225)
                     /gene="cas1c"
                     /locus_tag="PXD71_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HDL6291151.1"
                     /translation="MRKLQNTLYITTQGSYLHKERETLVVEQDRKKVAQLPIHSIGHI
                     FCFGNVLVSPFLMGFCGENNVNLAFFTENGRYLGRLQGRQSGNVLLRRAQYKKSETNP
                     VPVARNIIAAKIQASKRVLQRQIRNHGENADLQSTITALNYSLQQLKTADNLDLIRGI
                     EGDAAARYFGVFSHLIKENSGFHFDGRNRRPPRDGVNALLSFLYSIVGKDISGALQGV
                     GLDPQIGFLHADRSGRDSLAQDILEEFRAWWVDRMVLSLINRGQVKPDDFVTEASGAV
                     TLKPEARKLLFQTLQAKKQEKIIHPFLEEEVEIGLLPYIQAMLLARHLRGDLAEYPPF
                     LMR"
BASE COUNT         4538 a         3140 c         3097 g         4457 t
ORIGIN      
        1 gtagttcctt tttgatgtat caatactaaa aataacctgt attattctag cagatttctg
       61 ggtaatcgag gagttgataa catttaaaaa ttagcgtttt gcaaaatttt ccaaaaaaag
      121 accgcttgtt aggctataat agccaaccaa acagaccaat tcaaaggaga atcggtatgg
      181 aatatcaatt tacccatagt attcacgggg tggtggcaaa atgttcgatg gatcacgagg
      241 cgtttgctcg ttggttgaat gccgaaataa cggaaaatcc tagccagtta gcccctattt
      301 tcgctgaaat agagaagtgt cgagccgcat ttccgaatca ttatgaatgt gtgtttgaag
      361 ggcgggagta tagtctctat tttgattatg atgaagtgat ggcaaaagca aacaatttag
      421 atgctgcttt tgatgatgag gaaatggaag aaggctttca attttacaat gaagagagca
      481 tcgcattttg tggcttagat gactttgaga aatttttgaa agcctaccaa cattttgtgc
      541 aaacttatca ttgattgaaa tgattaattg aaaccaaagc tattaaggga atctaattcc
      601 ttgtagaata gccgtttata gcaattttaa gatattaaat ttatgagtcg tactaaaaaa
      661 acacgtcgta ttaccgacat tatgccggca agaaagaccg ataaacctaa acagcccatg
      721 cctaaattag gtggggggaa aaatcgcaaa ctgacccgtt atgagttaga tgctcaagcc
      781 cgtgaggaaa aacgtaagcg taaacataaa ggcttgccga caggctcacg caatgctgat
      841 ccggcagagc agaaaaaagc agtggtgaaa gaggtgaaag atccgcgtat cggtagccgt
      901 aaaaaagtgc cgttaatggt ggaatttgtg aaccagccgg aaaaagggcg gacgattaag
      961 gctgtgccgg ttgagccaat caaaccaacg ctttcaccgg aacaagagct ggagcagtta
     1021 gaaaataacg aatgtttaaa tcagttgtta gatgatttgg aggcgggcaa aaccttatcc
     1081 gcagaagatc agaaatttat gaatgaatgt ttagatcgca ttgatgaatt aatgactgag
     1141 cttggcatcg aatatgaaga tgaagagggc gataatggcg atgcgttatt acgccaattt
     1201 gaaacgatgg atattaataa attccgctag gtagccgatt atgatgctaa gaatattcct
     1261 gatggtgctt gccgctttga ttttaatttc gctgggcggg tatgcactgc acttaatgct
     1321 gaaagtgcga gctcagaaaa agcacgaaca agcactgatt gaacaggcaa agcaagcaca
     1381 aaaagatcgt tatattcgga ttttagacag cattgaagtt attgctcggg caatgatttc
     1441 agaacagtgc gatttttcgg aaggcgtttt acgtttaaaa ccgctattag atgttctcgg
     1501 cagaaaatta agcgactata cggcgatgtg ggcgttgtat ggcgtggtag aaaatatgcc
     1561 gattttagat gaacgcaaaa atttaaaacg taacgaaaga atgaagttgg atttagagcg
     1621 ggaagcaaaa gaagcggagc ttgaatcaga catcaaaaat gagtgttacc aattattaaa
     1681 agatattgaa gaaatgagaa aggcaatcta atgtcagaaa ttatttggga tttagccctt
     1741 attcaaaaat ataaccattc agggcctcgt tatacctctt acccaacagc gttggagttt
     1801 aacgaaaatt acaccaatga agattttatt cgtgctgcag ctcgttaccc tgagcgtccg
     1861 ctttcgcttt atgtgcatat tccgttttgc cataaactgt gctatttctg tggctgtaat
     1921 aaagtgatta ctcgtcatcg ccataaagtt gaaatttatt tagattattt agagcgtgaa
     1981 attaaaactc gcgcacctct gtttcaaaat cgtttagtga cccaaataca ctggggtggc
     2041 ggtacgccga cttatttaga cgaagatcaa tcggcacgtt taatgcaaat gctacgagcc
     2101 aattttgatg tgagcgatga tgcggaaatc agcattgaaa tggatccgcg tgaaatcgaa
     2161 ttaagtatgt tggatcacct gcgtgctatc ggttttaacc gtctgagtat gggtattcag
     2221 gatttcgata aagaagtgca acgtttggtg aatcgtgagc aagatgaaga gtttattttt
     2281 gccttgatga aacgggcaaa agaacttggt tttgtttcaa ctaatgtgga tttgatttac
     2341 ggcttgccaa aacagaatgt ggaaagcttt atgtacacct taaaacgtgt ggtagagcta
     2401 aaccctgatc gtatgagcgt atttaactat gcccacttgc caagccgttt tgctgcacaa
     2461 gtgaaaatta aagaagagtt gttgccggct ccggaaacca aattgacgat tttgcaaaat
     2521 tccatcgaat ttttaggcaa aaacggttat aagtttatcg gtatggatca ctttgctaaa
     2581 cctgatgatg agttagctat tgcacaagaa aaaggcattt tacaccgtaa cttccaaggc
     2641 tataccactc aagaagagtg tgatttattg ggaatgggcg tatcggcaat cagcttactc
     2701 ggcgatactt acgcccaaaa ccgcaaagaa ttacaacaat actatgctga tgttgaaacc
     2761 aacggtattg cgttgcataa aggcattgcg ttaacccaag atgattgcat tcgccgtgat
     2821 gtgataaaag ccttgatttg taatttcaaa ttagattttg ccaccattga aaagcaatat
     2881 ggtattgatt ttaaacgtta ttttgccgaa gatttagctt tcttagcgcc gttggcggaa
     2941 gatgaattat taacgatgaa tgacgacagc attgtggttt ccccacgagg gcgtttattg
     3001 atccgtaata tttgcttatg ctttgatgtc tattcaagac aattagcgag aagacagcag
     3061 ttttcccgta ttatttaatg agcaagcggt cgattttggt aaaaattttg caaaaacaga
     3121 ccgcttgttc ccggcttata aaggaaaaat gatgaaacag cgttatagct tactttctct
     3181 tgctgtgatg gcagcttgtt atagcaacag tgccgcagct gatttaaagc aacaatgttt
     3241 acttggcgtg ccgcattttc agggcgaagt ggtagagggc gatcaactta gaatgcctgt
     3301 taccattgag gctgacagtg cggtcattaa ccagcctcgt gatgccacct acaccggtga
     3361 tgtaacgatc aaacaaggta accgttcgat ttgggcggat caggtgcggg ttgagcaaga
     3421 tggcgaaaat gcccgaatgg cgtatttgca gggtaatttt aactaccaag ataatttgat
     3481 tcaggcaacc ggttcagatg cgaccatcaa tctgctttcc aaagatgcga atttagctaa
     3541 tgctaattac caattaattg gcagacaagg gcgtggcacg gcagaatccg gtgaattcgg
     3601 caacagcaaa cacacgctga aaaatgccag tttcacttcg tgcttaccga atgataattc
     3661 gtggcaaatt gaagcgaatg aaatgattca gcacgttgat gaagaatatg cggaaatgtg
     3721 gcacgcccgt tttaaagtgc tgggaatgcc ggtattttat tcgccttact tacaatttcc
     3781 gattggcgac cgccgccgta gtgggttatt aattccaagt tttgagcgtt caagcaaaaa
     3841 cggctggaca tattcgcaac cgttttattg gaatatcgca ccgaatatgg actcgaccat
     3901 cactccaact tactattcac gccgtggttg gcaaatcagc ccggaatttc gttatttaac
     3961 gaaaatcggg caaggcttag tggcaaccga atatatggcg aaagatcgtt taagcgagta
     4021 ccaaccggga aatgatgatc gtaaacgcta tttattccac tggcgtcacg caatgagttt
     4081 tttaaccgat tggcgtttct cggtcgatta caccaaagtg agcgacagac gttatttctc
     4141 tgattttgat tcggattatg gtagcagtac tgatggctat gcgacgcaga attttaaatt
     4201 aggctactat cagccgaatt ataatatctc aatttcaggt aaaaaattcc aaaccttcga
     4261 tgagttggat atcgggcctt atcgcgttct gccacaaatt gattttaact attataaaaa
     4321 cgatctaatt aaaggcggag atttcaaatt ctttgcccaa gcagcccgtt ttgaaaatga
     4381 tagtaaatta atgccaaaag cgtggcgttt ccacgctgag ccaacactga atttcccatt
     4441 agcgaaccgc tatggtagct taaatttaga aaccaagctc tatgcaacct actatcaaca
     4501 aacgaaaggt agtgagagta cggaagaaat taagaaaagt gtaagtcgtg tactgccaca
     4561 gattaagctc gattttaaaa ccgtgttgga atccgataaa cagcttttct ctggctatac
     4621 tcaaacgctt gagcctcggg tgcaatatat ttatcgccca tataaggatc aaagcgatat
     4681 tggctcgaaa gcacatcgaa gtgtgagctt aggctatgac tctgcattat tgcaaaacga
     4741 ctatttctca ctgtttaatg acagacgtta tagcggttta gatcgtattt cgtcagcaaa
     4801 tttagtcacc gcaggtggca caacccgttt ctttaaagag gcaaccggtg aagaggtgtt
     4861 taatttcagc ttaggacaaa cttattatat aagcccgtct aaaattgatg atacctcaga
     4921 taacagcact aaaggtcata cgacttcatg ggcagcggaa tctaactgga aattccataa
     4981 gaattggaat tggcatggta gctatcaata tgatactcgt ttaaaccaaa cggcgttagc
     5041 gaatatgtca ttgcaataca aaccacagtc agacaaattg attcagctta attaccgcta
     5101 tgcaagtaaa gattacatca atcaaaactt gaacacgaat aagtatgggc aatccattaa
     5161 acaagtgggc ggtgtgctag gttgggaatt aaccgataaa gtgtcggtaa tgaccagtta
     5221 ttaccacgac cttgcattga aaaaaccggt ggaagggcaa atttcgctta actacaacac
     5281 ttgctgttgg gcggcaaatg tgtatgtggc tcgtcgctta gtttcaacgc cggtaggttc
     5341 atctgatacg atcaatgatt tctactatga caataaattc ggcattaact ttgagctacg
     5401 ctttggcaca aactacagca atggtgtaac ccgtatgcta aaacgaggta ttattcctta
     5461 caccgagccg tatagcatta attaacaaag taaaaacaaa taagtaaaaa tacaaatcgg
     5521 ggcgaaaaat atttgccccg atttttttat atttggctga taagcggtta aattctgttg
     5581 gaattttgca aatcttccgc taaattcaac cgcttgccat cgaaacttaa cctgtatttc
     5641 gcatacctgc ggcaatacct gtgatggtga tcatcaatgc ttgctctagt acaggatcag
     5701 cattttcacc ttgtttgcga atcctatcta acaattctac ctgaagtaga ttgagcggat
     5761 cggtataaat attgcgtagt gcaatcgatt ctgcgatcca aggtaaatcg gccattaatt
     5821 gtccatcgtg agccagtgca agtacggttt taagatcagc ctgtagttgc gtacgcaaca
     5881 tattgcctaa acggtgtagt tttggctcga ctaggcgttg gtcgtaatat tccgccagcc
     5941 aaatatcagc tttactaaat accatttcca gcattccgat acgggtcgag aaaaacggcc
     6001 attcgttaca catttctttt agtaaggttt cattaccttc atcaatcaac tgttgcaatg
     6061 cttgccctgc acctaaccaa gcaggtagca ttaagcggtt ttgcatccaa gcgaaaatcc
     6121 aaggaattgc acgcaaactc tccactccgc cttttggatt acgttttgca gggcgtgagc
     6181 caagcggcag acgggaaagt tcttgctctg gtgtagcaga gcggaaatac ggtacgaagt
     6241 ctagctcgcc acgcaccata tcacggtaaa tttggcaaga aatatcggag gcttttgcta
     6301 gcacatctcg ccattgctgc tttggctccg gtggtgggaa aatattcgct tctaaaatcg
     6361 cactggcata gagttctaaa ctttcaactg ccacggcagg taagcctaat ttaaagcgga
     6421 tcatttctcc ttgttcggtc actcgtaagc catttttgag tgagcgaggt ggctgagaaa
     6481 gtaaggctgc gtgagccggt gcgccaccac gcccgatagt accaccacga ccgtggaata
     6541 aggttaattc aatattatat ttttcacata aattaaccag agcttcttgc gcccgatatt
     6601 gcgcccagcc ggcagcaaac attccggcat ctttggcgga atcagagtag ccgatcataa
     6661 tcatttggta attgttgatg acgccacgat accagccgat attaaataaa tcggtcatca
     6721 ctttttcact atgatctaaa tcgcctagcg tttcaaataa tggggcaacc ggaatagtaa
     6781 atttgcaccc cgcctctttg agtaaaaggt gaaccgagag tacatcagag gcctctcttg
     6841 ccattgaaat gacataggct gaaatgacac cttcaggttg ctcggcaaca attttacaag
     6901 tgtctaacac ttctttagtt tcagggcttg gagtccagtt ggttggaatc aatgggcggc
     6961 gagaactgag ttcacgcact aagaatgcct gtttatcatc ttccgtccag tgagcataat
     7021 cgcctaagcc gatatagcgg gtaatttctg caatcgccat ttcgtggcgg gtactttctt
     7081 ggcgaatatc taagcgtgat aaacctaaac caaaacagtt tatacgacgt aagcaatcta
     7141 gtaatgcacc atttgcaata attcgcatac cacaggcttg taatgactca tagcaatcaa
     7201 aaagcggttc ccacaattgc tgatctgaag ttaaaatgtc actttcatca atagttgaaa
     7261 ttttaccttc caataattcc gcatagtaaa tttcggtatt aattaatttc tggcgtaata
     7321 cttttagtaa tacgcgatat ggctctaaat ggttgccata tttagcacga aatgcagggg
     7381 tacactgtgt aatcgataat tcttcagcaa gatttttaat atcttgtaag aacaattccg
     7441 tggctttcca acggttcatt cttaatacac ggcgagttgt ttctgccgtg acgaacgggt
     7501 taccgtcacg gtctccgccc atccaagaag aaaaacggac aggggctaaa tttaccggat
     7561 attgcacacc aaagtgtttt tctaaataga agttaagctg acggcaaaat tccggcacgg
     7621 ctttccataa actattttca atgactgcca taccgccttt ggcttcatca acaggggttg
     7681 ggcgttgggt tctgatttca ttagtatgcc aagcaagggc aatcagttgc attaaacggc
     7741 gcttgatttt atagctttct gcctcagtta aatcatcatg ttccaacaaa ttcaaacatt
     7801 tattgatttc aacgtgttta tgccccagcg aacggcgggt tacttccgtt gggtgagctg
     7861 ttagcactaa atcaatcatt aggctttcca cggttttaat cactttttca acggggatat
     7921 tttgtgattt tagacgaagg aacagagagt ctaatgagcg actaccaagt gattcatcaa
     7981 tatgatggcg agaaacagtt tgatgttgtt cagcaatatt tgttagattt agaaaatggc
     8041 taaatgcacg tgcaacgggt aagacatcat cgttagaaat attggctaat ttatctaata
     8101 gctgttcacg ggcgttttca tcgccggcac gagaattgcg agataacaca cgaatgcttt
     8161 cgatgagttc tagaatgtaa tttccttgcg catcacgaat cgtttctccc aaaaattcac
     8221 ctaacatatg gatattatgc tgaattgttg aatattgctg gttcataaaa aattcctatg
     8281 gtaaacataa attaaattag gtctaaatat gccgaaaatc gctgaaaata gcaaatagat
     8341 ttaatttaat ttttgaatta aatgataaaa cgcaaaaaat agctgtcaat atgtgtttaa
     8401 aactgttgtt cggttttgat aacttgcctg ttgtttatgt gctattcact gttattcaag
     8461 ttggcttatc cattaatggt tttaaaggta ttaaatggtt ggaaaatgtt tcttgtgtgt
     8521 tcctcatcgg tattcttgct tatatgcttt atgttgtgaa tacaaaattc tctgtagaaa
     8581 taggcgagcg ctttgccaat attgaaggca cttggggaat gccattctgg gctgcaacta
     8641 cagcattctt gggtatttat tcaacaatga ttttaaatgc ttccgattat tctcgccacg
     8701 ttaaaaaagg aacaacccca acagcaacag gtgtgattta tgtattatca atcctccctg
     8761 taaccttatt tatgggttta atagggttgt tagttacggc agctacaggc aatagtgacc
     8821 caattgaagt atttgcaact gcaatggata ataaattttt aacagtaata acgttattat
     8881 tcattgcttt tgcacaggtt acgacaaacg tgttgaataa tattgttccg ccatcttaca
     8941 ttttaatgga ttcatttaaa atgaaatggt cgcatgccac cattttagtg ggcattcttt
     9001 ctatatgtgt aatgccttgg aaattggtaa ctgctgaatc agcagatgga ttatctttgt
     9061 ttattaaaat ttactcggca ttcttaggcc ctatttttgc ggtaatggtc gtcgattact
     9121 acatcttaag aaaacgtact ttaaacgtaa atgacttata taataaagaa ggtattttca
     9181 aagggattaa ctgggcagca attattgcga ttgctatcgg ttcgttttgc tcactgattg
     9241 tggtggatct ctcttggtat gtcagtttaa ttccatcagg cttgagctac tatattttga
     9301 tgagggtgtt aaaaagttcc gaagctttcc gtaaagggac agtgctagaa catcaataag
     9361 atgacaagcg gtaaaattta ttaaaaattt gaccgcttgt aatacaaaaa agcacaccga
     9421 agtgtgcttt tgttctattt ctgattactt ctgacgcatc gctgggaata aaattacatc
     9481 acggattgat ggggcgttag cgaaaatcat tactaagcgg tcaattccta aaccttcacc
     9541 cgctgttggc ggcagaccgt gttccaatgc cacgataaag tcttcatctt taaacattgc
     9601 ttcatcatca ccggcatctt tcgccgcaac ttgagcatca aaacgctctg cttggtcttc
     9661 tgcatcgttt aattctgaga aaccattacc aatttcacga ccaccgataa ataactcaaa
     9721 acgatcagtt acctctgggt tttcatcatt acggcgagct aatggtgaaa tttctgccgg
     9781 gtgagccatc aagaaagttg gctgaattaa ttgatgttct gccacttctt caaaaatcgc
     9841 attgactacc gagcctaaac cccaagattt ttgaatttca atgcctaagc ctttcgcaat
     9901 ttccacactt ttctcgaagc tgtctaaatc ttcacgttga atgccgttac catatttcac
     9961 aatcgcatcg tgcatcgtaa tacgctcaaa cggtttaccg aaatcgaata cataatcacc
    10021 ataaggtacg tcagttgtgc caagaatatc taaggctaat tggcgtaaca gttcttcggt
    10081 attatccatt aaatcgtgat aatccgcata ggcttggtag tactcaatca ttgtaaattc
    10141 aggattgtgg cgtactgaaa ccccttcgtt acggaagtta cggttaagct caaatactcg
    10201 ctcaaaacca cccacaacta aacgttttaa gtaaagctct ggagcaatac gcagatacat
    10261 atccacatct aacgcattat gatgagtgat aaacggtttc gctgccgcac cgcccgggat
    10321 cacttgcaac attggcgttt caacttcaat aaagtctctt tctaagaaga atttgcgaat
    10381 gcctgaaact acttgcgagc ggatcataaa agtacggcga gattcttcat tagaaatcaa
    10441 gtctaaataa cgctgacgat aacgagtttc taaatccgtt aaaccgtgat gtttatccgg
    10501 caacgggcgt aaagacttgg ttaaaagttg aacttcagag caacgaaccg ttaattcacc
    10561 ggttttggtt ttaaataacg caccttcgac gcccacaatg tcgcctaaat cccaaaggct
    10621 gactttttct tcgtaaacgc tgtcacttaa gttatcacga gctacataaa gctgaatcgc
    10681 accgctcaca tcttgaatgg taaagaaaga tgccttaccc attactcgtt ttaacataat
    10741 acgaccggca actttcactt gaatattctg ttcttttaag gtttcacctt ccactgcatc
    10801 atattgagcg tgcagatctt tggctaacgc atcacgacgg aatgtatttg gaaatgcgtt
    10861 accctgctca cggattttgt ttaatttttc acggcgggct aacatttcac cgtttagatc
    10921 taattcttga ttttctactt cagacattga attaccttag atttcaaaaa aggataaata
    10981 aaactggacg attatacgtt attttgagga atttggatag gaaacaagcg gtcagatttt
    11041 ctaaattttt tgtaactaac gatattgagg ctaactattt agcctcaacc aagtaataga
    11101 ttacatcacg accacatcaa actgctcttg atgataatat ggctcaagtt taatctcaat
    11161 ttttttaccg ataaacgcct gaagctcagc caataatgcg tggctttcct cgttaattaa
    11221 atattcagca acatctttag ctgcataaac gtgaatattt tctgtcttaa acaaatgatg
    11281 tacacgcaca atttcacgca taatttcata gcaaaccgtc tctacagttt tcactgtgcc
    11341 acgcccttgg caagcttggc aatcgcaaca aagaattcgc tctagactct cccttgtacg
    11401 ttttctggtc atctccacca agccaagctg agtaaagcca ttcacattgg ttttaacacg
    11461 atcgcctttt aatgcctctt gtaacgattg taaaacgcgc tcacgatgtt cttcctcttg
    11521 catatcaata aaatcaataa tgataatacc acctaaatta cgcaactgta gctggtgtgc
    11581 aattgcctgt attgcttcaa tatttgtgtt gaaaatcgta tgtgcaaggt tacgatgtcc
    11641 gacaaaagca ccagtattaa tatcaatagt cgtcatcgcc tcagtttgct caataattaa
    11701 atagcccccc gattttaaat caacccgttt atccaatgcc ttttggattg ccttttctac
    11761 accataggca tcaaataaag tttgctgacc ggtatagagg gtgacacaat ccgttaattc
    11821 tggcataaac tcagctaaaa attctcgcac ttgctcataa gttagctttg aatcgattct
    11881 aaccgaattg atatgtgtac caataaaatc acgcagtacc ctttgagcaa gcgcaagctc
    11941 tccataaagc attgattttg cggaatattt cccttttcgt tctaatactt tacgccacaa
    12001 ccgttttaaa aattcaacat cttgctttaa actttcttca gttgcctctt ctgcagcggt
    12061 acgtacaata aagcccccaa gttcatcaca ataaggctca accaagcttt ttaaacgagc
    12121 acgctcttct tcactttcaa tccgctgaga tactcccaca tggctatttt ctggcataaa
    12181 cactaaataa cgagatggta acgtaatatc agtagtgagc ctagctcctt tagtaccaat
    12241 tggatctttc actacttgta caactatatc ttgcccttca cgcaccaatt cagaaatatc
    12301 tttaaccaga aattgcttct tttcattttc atcaacacat tccgtatgcg aaacgatgtc
    12361 agaagcgtgc aaaaaagcag ccttctccaa gccgatatcc acaaaagcag attgcatacc
    12421 tggtaatacc cttgtcaccc ttcccttata aatattacca acaatcccgc gtttagcttc
    12481 ccgctcaata tgtaattctt tcaatattcc gttttctacc aacgctaccc ttgtttcgct
    12541 tggggtaaca ttcactaata attccgtact actcatctta ctgtctctat attttaaaac
    12601 tatctaaagt gtatcatttc acatcttaga aaacaaaaac tcagttcgaa caagcgggta
    12661 atttctgatt attttttgca aaatagaata ttttcgtcat tactatgata catagcagat
    12721 gcaatcagtc taattaacaa catacaaatt tgccattgat atttcaatat atattgatgc
    12781 gaaggtagca gccaatggcg aaaacatagg atttgtgggt acaccgtttc aatacacagc
    12841 cacgcgaagg tggctgccta ttgtcgccct tgattttcca attcaagacg aggtttcaat
    12901 tcacagccac gcgtaggtgg ctgcttggtt ataattaaaa ttgattgcgg tacacgcccc
    12961 tgtttcaatt cacagccacg cgtaggtggc tgcggggatt aaccgaacag gattggattc
    13021 gttatgaggt ttcaattcac agccacgcgt aggtggctgc gcgtagctaa tggcagattt
    13081 ttacggcaat ttagggtttc aattcacagc cacgcgtagg tggctgcctc tagcattaaa
    13141 atccaatcgg taaacggtaa agtttcaatt cacagccacg cgtaggtggc tgctttaatt
    13201 aataaaatag ctttagcttt agatttaagt ttcaattcac agccacgcgt aggtggctgc
    13261 ccaacaatgc ttttctttgt ataaccagcc acgagtttca attcacagcc acgcgtaggt
    13321 ggctggtatc atttatcgcc cgaaaaatcc aaaaatcgca gtttcaattc gcagccacgc
    13381 gtaggtggct gggtaaaaga ttactactac cctatggcaa taagtgtagt ttcaattcgc
    13441 agccacgcgt aggtggctgg cgcaagcatt gcaccacggt aacaacggct gctggtttca
    13501 attcgcagcc acgcgtaggt ggctgggcgt ttatcagcat ctgctcgtgt tactgtgcca
    13561 cgtttcaatt cgcagccacg cgtaggtggc tgggtgcaaa ctactccgat aaccgaagta
    13621 gcagcggtgg tttcaattcg cagccacgcg taggtggctg gtccctataa gataacgtat
    13681 tgaaatatca ggctttttag ccctttattc gctaacgttt tgtagagagt ggataaagta
    13741 tagcatatcg ccaatatatt gtgccttatt ttttctaatt ccttgattat taaagaacaa
    13801 tatagattcg ctaaccttgc tacattttta tgagaactac aggctagcga tttataaaat
    13861 cagcgtatct ttaaaaatat ccactgaagg ttttgcgcca tggtgctcaa ctttattcct
    13921 ccatttactg cctaaatggt aaaagcgcaa actatcacag tctgggttgt aagttgccaa
    13981 taatttattt ttcaatgtta cccactgagc aggagtaaca tcacactcaa acacagaata
    14041 ttgcgcccgc acaccataat ccaaacaatg cttagcaata cggcgtaggc gagcctgccc
    14101 ttcggggtca tcaaatgaaa tgtcgtaggt aatcaacatc agcatagttg ctaattcctc
    14161 aatgaataaa acataaacaa gcggtcaaat ttcaacaaaa ttttgcagat tttaacgcat
    14221 taaaaacggc ggatattctg ccaaatcgcc acgcaaatga cgagccaata acatcgcttg
    14281 gatataaggt aacaagccga tttccacttc ttcctctaaa aatgggtgga tgattttttc
    14341 ttgttttttc gcttgcaagg tttgaaatag taattttctg gcttcaggct tgagcgttac
    14401 cgcaccactg gcttccgtca caaaatcatc aggcttaacc tgccctcgat tgatcagcga
    14461 cagcaccatt ctgtccaccc accacgctcg aaattcctcc aaaatatctt gagctaagct
    14521 atctcgcccc gaacgatctg catgcaaaaa gccaatctgg ggatcaagcc ccacgccttg
    14581 caatgccccg ctaatatctt tccctacaat gctataaaga aatgaaagca acgcattcac
    14641 accgtcacga ggtgggcggc gattacgtcc gtcaaagtgg aaaccgctat tttctttgat
    14701 aagatgagaa aacaccccaa aataacgagc cgcagcatcg ccctcaatgc cacgaatcaa
    14761 atccaaattg tctgctgttt tcaactgttg caggctgtaa tttaatgcgg taattgtcga
    14821 ttgaaggtca gcattttcgc cgtgattacg aatttgccgt tgtaacaccc gcttagatgc
    14881 ttgaattttg gctgcaatga tgttgcgagc cacaggaaca gggttggtct ccgacttttt
    14941 atactgggct cggcgtaaca atacattacc actttgccgc ccctgcaacc gcccaagata
    15001 acgcccattt tcggtaaaaa acgctaaatt tacgttattt tcgccacaaa atcccattaa
    15061 aaacggcgac actaacacat tgccaaaaca aaaaatatgc ccaatggaat gaatcggcaa
    15121 ctgtgccact ttcttccgat cttgctccac caccaaggtt tctcgctctt tgtgcaaata
    15181 gctaccttgg gtggtaatgt agagggtgtt ttgtagtttg cgcatggttg aa
//
LOCUS       DANLXS010000045        14990 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.045, whole genome shotgun sequence.
ACCESSION   DANLXS010000045 DANLXS010000000
VERSION     DANLXS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14990
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.045"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            88..816
                     /gene="fabG"
                     /locus_tag="PXD71_001013"
     CDS             88..816
                     /gene="fabG"
                     /locus_tag="PXD71_001013"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL6291152.1"
                     /translation="MNNTVLITGSSRGIGKAIALTLAQQGFDIVVHCRSRVEEAEAVA
                     QAVRNLGKNARVLQFDVADREKVRSILEADVEQHGAYYGVVLNAGLTRDNAFPALTDE
                     DWDSVLRTNLDGFYNVLHPIMMPMIRRRKAGRIVCITSVSGLIGNRGQVNYSASKAGI
                     IGAAKALAIELAKRKITVNCVAPGLIDTDILDENVPIEEILKMIPAGRMGDPDEVAHA
                     VAFLMDEKATYITRQVIAVNGGLC"
     gene            879..2024
                     /locus_tag="PXD71_001014"
     CDS             879..2024
                     /locus_tag="PXD71_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL6291153.1"
                     /translation="MKRVVITGIGVVTAFGKEWAEIHGYQPPKHWNRKQLRSLGRVSQ
                     FVVEASERALAQAGLLDENGVIFDWLKDRRMGVACGSSTGSTNDIKDAAELLMTGKSD
                     GFNANTYVRMMPHTTAANIGIFFGLTGRIIPTSSACSSGSQGIGYAYEAIKYGMIPMM
                     LAGGGEEFCASKVYVFDSLYAASRRNDNPKGTPRPYDKDRDGLVIGEGAGMFVLEELE
                     HALARGANIIAEVVGYGANSDGSHVTRPQATTMQRCMELALKDAGIQPNQVGYVNGHG
                     TATEQGDIAETQATTAVFGKIPLSSQKSYLGHTLGACGALESWFSIEMMRDGWFAPTL
                     NLENVDERCGDLDYLQGDGREIHTDYVMNNNFAFGGVNTSLIFKRWK"
     gene            2038..2943
                     /locus_tag="PXD71_001015"
                     /pseudo
     CDS             2038..2943
                     /locus_tag="PXD71_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
     gene            2966..4108
                     /locus_tag="PXD71_001016"
     CDS             2966..4108
                     /locus_tag="PXD71_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDL6291154.1"
                     /translation="MLSNVYITNTSAFLPNAPVSNDAMEAVLGMVGETPSRVRKMILR
                     SNGIQHRYYAIDPITRQATHSSTELAVQAVKKLGNSATSATCLAVGTSYPDQIMPGQG
                     VMVHGLLDAEPMEVLTTAGVCVAGMAAMKHAYNAVRIGEHRAAIATASETASAIMRSE
                     HFRAEIDHKKLDDARPEIGFEKDFLRWMLSDGAGAVLLANQPNEKGLSLKIHWIDLVS
                     YANEMPVCMYAGAEFIDGNYTGWKAMSEAQRAECSAMAVKQDVKLLNENIVKYTVEKA
                     LERIIPKYKLNAEEIDYFLPHYSSGSFRDKLLEGLQRINFLIPQEKWFTNLTTKGNTG
                     SASIYIMLDEFIRTHDLKQGQKILCYVPESGRFSSCFMLLEAVVNS"
     gene            4157..4372
                     /locus_tag="PXD71_001017"
     CDS             4157..4372
                     /locus_tag="PXD71_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL6291155.1"
                     /translation="MDINDIPQDNSKIFNGERKVIYARNPQPEIRQMISSILACYAWD
                     ENNPFVAKSDARLKALAEVVGKAEIGD"
     gene            4653..5144
                     /gene="rplJ"
                     /locus_tag="PXD71_001018"
     CDS             4653..5144
                     /gene="rplJ"
                     /locus_tag="PXD71_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL6291156.1"
                     /translation="MALNLQDKQAIVAEVNEAAKGALSAVVADSRGVTVEKMTELRKA
                     AREAGVTMQVVRNTLLRRAVEGTEFECLTDTFTGPTLIAFSNEHPGAAARLFTEFAKT
                     NKEFELKGAAFEGKKQDVAFLATLPTYEEAIARLMGTMKEAAAGKLVRTLAALRDQME
                     AAA"
     gene            5203..5571
                     /gene="rplL"
                     /locus_tag="PXD71_001019"
     CDS             5203..5571
                     /gene="rplL"
                     /locus_tag="PXD71_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL6291157.1"
                     /translation="MSLTNEQIIEAIASKSVSEIVELITAMEEKFGVSAAAAVAAAPA
                     AGAAAAEEKTEFDVVLAEAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGISK
                     AEAEALKKELEEAGAKVEIK"
     gene            complement(5678..6508)
                     /locus_tag="PXD71_001020"
     CDS             complement(5678..6508)
                     /locus_tag="PXD71_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL6291158.1"
                     /translation="MVQKPLNHYLEMQEHFLYVPYLHHHRRIRVLLPKDYHNEGWERY
                     PVLYMHDGQNVFYSKESYSGHSWKIIPTIKSHQELPKMIIVGIDNAGANRLNEYGPWK
                     TTTGSTPETRNAGGLGEEYAKWVVETVKPFIDIHYRTKPQQEHTLLAGSSMGGIITAY
                     MGSRYPHIFGHLGVFSLASWFSEPDFLRFTHQYPLQPNTKVFIQVGTNEGDEIDSHFI
                     SNTNQTYIDCSLNYYQALIRIGVPLDNIRLRIMANEIHHEMHWADHFVEFLHFSLLRK
                     "
     gene            6734..8275
                     /locus_tag="PXD71_001021"
     CDS             6734..8275
                     /locus_tag="PXD71_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDL6291159.1"
                     /translation="MQASLQDILEVVKAKNLTYQQKLMSLGNIAERLFNPIELLGYTE
                     EEWEHIENQMICDLNEGYAIYRPRYILPDYNVYMQKGCQFLDLPPPTNLDEALDGLLI
                     LYSHVPSITTFPVYIGRLDQLLEPFITDEEQDYIKIKRFLNHIDKTISDSFCHANIGP
                     NDTKAGRLILKAVIELENITPNMTIRYDKTKTSREFAELAAKACLLVSKPSFANDAYY
                     IQDLGEEYGIASCYNALPECGGAYTLTRLRLGTIGRACASVEQMVNEMLPKVAKLALS
                     TMDKRHKFLVEESNFFESSFLVQEGFIKRTNFTSMIAIVGLADAVNHLLKQEGLNETF
                     GQSKRGDEIATLIMDKLQAVNDAHKGLYVERTNNQYLLHAQVGANNHAEDKMNTPAHR
                     IRVGEEPTLLAHLKQSAPFHKYFPSGTGDLFAFDQTYTDHLDAVVDIIDGSFAQGYRY
                     ITTYLKNTDLIRVTGYLVKKSEVERYRKGEAVLRDTTWYGSGTDDCAKVFDRQLRDEE
                     NVNAG"
     gene            8278..9066
                     /locus_tag="PXD71_001022"
     CDS             8278..9066
                     /locus_tag="PXD71_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HDL6291160.1"
                     /translation="MAALSEIFVPLHRIIPFSNVEGQGNRTSIFLQGCKLNCLYCHNP
                     ETIPRYSKEAKQVSLQYLYDQVMEAVPFIRGVTISGGEPTIHHKKLVPLFEALREQGL
                     TCYLDSSGFFDFEATEPLINVTDKFLFDLKGDGAGLQTLCFDRKNQAGKVPEQALPNV
                     NHIKPENLARNLQNLAKLLPLDKVEEVRLVYLNEFYNAHHLLEKVADLLKPYPDVLLK
                     IIRVHTKGARDETGLARFVPTIEQTDELAAFAKRHGIQKVITIY"
     gene            9079..10336
                     /locus_tag="PXD71_001023"
                     /pseudo
     CDS             9079..10336
                     /locus_tag="PXD71_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(10415..12424)
                     /locus_tag="PXD71_001024"
     CDS             complement(10415..12424)
                     /locus_tag="PXD71_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6291161.1"
                     /translation="MSAENMPSVIRFEQAVAKKDYESACTELLSILSKLDSNFGGISN
                     IELNMPEQIENLENDKAIYFCTRMAVAITRLFEDPALEISEHGAMRFLTLQRWIALIF
                     ASSPYVNADHILRTYNRNKESANPNTVDLDATLQALIKFCILYLPESNILLNLDAAWN
                     ASSDLTASLCFALQSPRFIGTSSAFAKRAAILQWFPEKLAQIENLNKLPSAISHDVYM
                     HCSYDIEANKHNVKRSLNAVIRRHLLSVGWEDRKIEQLGTRNNKPVMVVLLEHFHSSH
                     SIYRTHSTSMVAAREHFHLIGLGSDAVDEMGQQVFDEFHLLPQDGSLFDRLSFLKDIC
                     DKNNPAVFYMPSIGMDLTTIFASNTRLAPIQAVALGHPATTHSDFIEYVIVEDDYVGS
                     ESCFSEQLLRLPKDALPYVPSALAPQNVVYNLRENPEVIHIGIASTTMKLNPYFLEAL
                     KAIRDRAKVKTHFHFALGQSSGITHPYVERFIKSYLGNDATAHPHSPYDEYLNILHNC
                     DMMLNPFPFGNTNGIIDMVTLGLVGVCKTGPEVHEHIDEGLFKRLGLPNWLITQTAEE
                     YVTQAIRLAENHEERLAIRRDIIENNKLQTLFSGDPRPMGQIFLAKVQAWLADKNSKN
                     AEVEVKTKKVRKAATASQSAKKQTTSKTQTAKAEKDNTAKTETKS"
     gene            complement(12434..13771)
                     /gene="rimO"
                     /locus_tag="PXD71_001025"
     CDS             complement(12434..13771)
                     /gene="rimO"
                     /locus_tag="PXD71_001025"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDL6291162.1"
                     /translation="MSASPNIGFISLGCPKNLVDSERILTELRADGYNIIPSYENADL
                     VIVNTCGFIDSAVQESLESIGEALEANGKVIVTGCLGAKENQIREVHPKVLEITGPHS
                     YEAVMNHVHKYVPKPEFNPYTSLVPKQGIKLTPKHYAYLKISEGCDHRCTFCIIPSMR
                     GDLASRSIVQVLDEAKRLVDAGVKELLIVSQDTSAYSLDRKKEEATKTVFWNGMPIKS
                     NLITLCEQLGSMGVWVRLHYVYPYPHVDDLIPLMAAGKILPYLDIPLQHASPKILKAM
                     KRPGSIERTLERIKKWREICPELTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGC
                     FKFSPVEGAVATDMPDQVPEEVKEERFHRFMQLQQEISAARLQAKIGKTVQVIIDDID
                     EEGIIGRSMADAPEIDGVVYVDNPSNQLVTVGQIISVTITHADEYDLWGILNN"
     gene            complement(13926..14618)
                     /gene="mtnN"
                     /locus_tag="PXD71_001026"
     CDS             complement(13926..14618)
                     /gene="mtnN"
                     /locus_tag="PXD71_001026"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL6291163.1"
                     /translation="MKIGIIGAMAQEIEILRQVMVEPKVTELAGCKIFEGKINNTRVA
                     LLQSGIGKVAAAIGTTLLIQLTQPDMIINTGSAGGLDPDLNVGDILISTEVRYHDVDV
                     TAFGYEIGQLPANPPTFSPNEQLVELAQKEAKKAGYNVVSGLICSGDAFINGNDKINQ
                     IRQNFPNVVAVEMEAAAIAQVCHGFNLPFVIVRAVSDVADKESHLSFEEFLPLAAEKS
                     STIVLAMLNNID"
     gene            complement(14676..14897)
                     /locus_tag="PXD71_001027"
     CDS             complement(14676..14897)
                     /locus_tag="PXD71_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HDL6291164.1"
                     /translation="MDNNYTLSAKITELETKVAFQELIIEELNQALIEQQFAIDKLQT
                     QIRHIAEKLKGAQLSQVASQAEETPPPHY"
BASE COUNT         4296 a         3095 c         3339 g         4260 t
ORIGIN      
        1 aacaaaaaaa tattctcact tggcacggct taagtccaaa agatccaacc caatactaca
       61 cactcaagaa atagtaggaa atataagatg aataatacag ttctcatcac cggctcaagc
      121 cgtggtatcg gtaaagcgat tgctttaacc cttgcacaac aagggtttga tattgtggta
      181 cattgccgtt ctcgtgtgga agaagcggaa gcggtggcac aagcggtcag aaatctcgga
      241 aaaaatgcca gagtgttaca gtttgatgtg gcggatcgtg aaaaagtgcg gtcgatttta
      301 gaggcagatg tggaacaaca tggtgcctat tacggcgtgg tgctaaatgc agggcttacc
      361 cgtgataacg cctttcctgc tttaaccgat gaggattggg atagcgtgtt gcgtaccaat
      421 ttagacggtt tttataatgt cctgcacccg attatgatgc cgatgatccg tcgccgtaaa
      481 gcaggcagga ttgtctgtat cacctcggtt tccggcttga tcggcaaccg tgggcaggtc
      541 aattacagcg cttcaaaagc cggcattatt ggtgcggcaa aagcactcgc catcgagctt
      601 gccaaacgta aaatcacggt aaactgcgtg gcgcccggct tgattgatac cgatattctg
      661 gacgaaaacg tcccaatcga agaaattctc aaaatgatcc ccgcaggacg aatgggcgat
      721 cctgatgaag tggcacacgc cgttgcgttt ttaatggatg aaaaagccac ttacatcaca
      781 cgccaagtca tcgctgtgaa tggtgggttg tgttaacttt aggataataa ttcgtagggg
      841 cggatccagt acccgcccga agccaaaaag agataaatat gaaacgagtc gtcatcaccg
      901 gaattggtgt ggttaccgct tttggtaaag agtgggcgga aattcacggc tatcaaccgc
      961 cgaaacattg gaaccgcaag cagttgcgca gccttgggcg agtgtcgcaa tttgtggtgg
     1021 aggccagcga gcgggcttta gcacaggccg gtttgttgga cgaaaacggc gtgattttcg
     1081 actggctaaa agacaggcga atgggcgtgg cgtgtggctc gtccacaggc tctaccaatg
     1141 acatcaaaga tgccgccgaa ttgctgatga ccggcaaatc ggacggtttc aatgccaaca
     1201 cctatgtgcg aatgatgccc cacaccaccg ctgccaatat tggcattttc ttcgggctga
     1261 cagggcggat tatcccgact tccagcgctt gctcctcggg cagccaaggt atcggttacg
     1321 cttacgaagc cattaaatac ggtatgatcc cgatgatgct tgccggcggt ggagaagaat
     1381 tttgtgcctc caaagtctat gtcttcgaca gcctctatgc tgccagccgc cgcaacgaca
     1441 acccaaaagg cacgccacgc ccctatgaca aagaccgaga cggcttagtg atcggcgagg
     1501 gagcgggaat gttcgtattg gaagagctgg aacacgcgtt agcacgaggg gcaaacatta
     1561 tcgccgaagt ggtgggctac ggtgcaaaca gtgacggttc ccacgtcacc cgcccacaag
     1621 ccaccacaat gcaacgctgt atggaacttg cccttaaaga tgccggtatt caaccgaacc
     1681 aagtcggcta tgtaaacgga cacggcacgg ccaccgagca gggcgacatt gccgaaactc
     1741 aagccaccac agcggtgttc ggcaaaattc cgctcagctc gcaaaaaagc tacttagggc
     1801 atacgctcgg tgcgtgtggt gcattggaaa gctggttcag cattgaaatg atgcgagacg
     1861 gctggttcgc cccaacactg aatttagaaa atgtcgatga acgctgtggc gacttggatt
     1921 accttcaagg tgacggacgg gaaatccaca ccgattatgt gatgaacaac aatttcgcct
     1981 tcggcggtgt gaatacctca ttgattttta aacgttggaa ataaccgttt tcaaataatg
     2041 aaaaagatat tattagttta ttactcccaa accgggcaac tcacccattt ggcagaaaat
     2101 tttgtacaat cgcttgaaca agcgggcgtt tttgtggaaa aactcgcaat caaaccgcaa
     2161 caggaatacc cgtttccgtg gcggtttatg cgttttttta acaccttccc cgaaaccgtc
     2221 cacctcacac cgccgccgat tgagcctctg ccctttcagc acgaaatata cgatttggtg
     2281 atcatcgcct attccgtgtg gtttctctcc cccagccagc cgattaccgc ttttttacag
     2341 agtgaacagg caaaaaagtg ttgaaggata cgcccgtcat cacgctgatc ggctgtcgca
     2401 atatgtggct gatggcgcag gaaaaagtga aaaaactgct tgccgagagt gaggcgaagc
     2461 tcattggcaa cgtggtcaaa gtggatcaat ctaacgactg ggcgagtttt atcaccacac
     2521 cgctttggat gctgaccggc aagaaaaaaa atcggcaagc tgccgagcgc tggcattgcc
     2581 gaaagcgaaa tggaagatgt aaaacgcttc ggggcaaaac ttttgcacat ttttagccaa
     2641 aaccaaccgc ttgacagcac ccttttccgc caaatgcaag cggtcaaaat tgacgaaaaa
     2701 ttgatgatga gcgaaaaagt cggtgcgaga agtttccacc tttggggcaa attactgatc
     2761 aagtgcggtc aaatttcacc gcacttacgc caagcggtac tctattttta catcctcttt
     2821 ttagtgacgt taattttaac cgtcgtcccg atttcaacgg tgattaaacg gctattacgc
     2881 ccactgctga aagaaaaatt agaaaaacaa aaacggtatt ttgccgagcc ttcgggcgag
     2941 taaacgtacc ctacacggaa actttatgct atctaacgtc tatatcacca acacctccgc
     3001 ctttttaccc aacgcccctg tgtccaacga tgcaatggaa gcggtgttgg gaatggtggg
     3061 ggaaacgcct tcccgagtgc ggaagatgat tttgcgttct aacggtattc aacatcgtta
     3121 ttatgccatt gacccgataa cccgccaagc gactcacagc agtaccgagt tggcggtgca
     3181 agcggtcaaa aaattgggaa attctgcaac atcagcgact tgcttggcgg tggggacatc
     3241 ttaccccgac caaattatgc cggggcaggg ggtgatggta cacggcttgc ttgatgccga
     3301 accgatggaa gtgctgacca ctgccggtgt gtgcgtggcg ggaatggcag cgatgaagca
     3361 cgcctataat gccgtacgca tcggtgagca tcgtgctgcg attgcaacgg cttccgaaac
     3421 cgcctccgcc attatgcgca gtgaacattt ccgtgccgaa atcgatcata aaaaactgga
     3481 cgatgctcgc cctgagatcg gttttgagaa agatttcctg cgttggatgc tctcagacgg
     3541 tgcaggggcg gtattgcttg ccaatcagcc gaacgaaaaa gggttgagcc tcaaaattca
     3601 ctggatcgat ctggtctctt acgctaacga aatgccggtt tgtatgtacg ccggtgcgga
     3661 atttattgac ggcaactaca ccggctggaa agcaatgagc gaagcccaac gtgccgaatg
     3721 ctcggcaatg gcggtgaaac aagatgtcaa actgctcaac gaaaacatcg tcaaatacac
     3781 ggtggaaaaa gcattggagc ggattatccc gaaatacaag ctcaacgctg aagagattga
     3841 ctacttcctg ccgcactact cgtccggttc cttccgagat aaattattgg aaggtttaca
     3901 gcggatcaat ttcctgatcc cacaggaaaa atggttcacc aacctgacta ccaaaggcaa
     3961 cactgggtca gcctcgatct acattatgct cgatgaattt atccgcaccc acgatctcaa
     4021 acagggacaa aaaatcctct gctatgtgcc ggaaagcgga cgtttttcgt cttgttttat
     4081 gcttttggaa gcggtggtaa attcgtaaaa tcgtagggac gggggctatt cccgtccaac
     4141 aatcatcgga aaacgtatgg acatcaatga cattccccaa gacaacagca aaatcttcaa
     4201 cggcgaacgc aaagtgatct acgcccgcaa tccgcaacca gaaatccgcc aaatgatctc
     4261 ctctattctc gcttgctacg cttgggacga aaacaacccg tttgtagcga agtctgatgc
     4321 acggttgaag gcattggcgg aagttgttgg taaagcagaa attggcgatt aagtttgatt
     4381 ttgtgcttta cagggtcgca gattatctgt ataatttgcg accttaactt gcgagagcaa
     4441 gctgatggtg cttagcctat ctaagccccg tctaagactg taggggtgaa aacttaataa
     4501 tcctacatag atggtgaaca gacagaattc tctgcttctg gacaccttag gctcaaagaa
     4561 gattgcagtt tgtagcaatg caagcggtaa tatttttggg attttttgta aattctgctt
     4621 gaaaaggcag agtgtatcag gagctaaaac caatggcatt aaatcttcaa gacaaacaag
     4681 cgattgttgc tgaagtaaac gaagctgcca aaggtgcact ttcagctgtt gttgcggatt
     4741 ctcgcggtgt aacagttgaa aaaatgactg agttacgtaa agcagctcgt gaagctggtg
     4801 tgacaatgca agttgtacgt aatactttat tacgtcgtgc agtggaaggc accgagttcg
     4861 agtgcttaac agatacgttt accggtccaa ctcttatcgc attctcaaat gaacacccag
     4921 gtgcagcagc acgtttattc actgaatttg cgaaaacaaa caaagagttt gaacttaaag
     4981 gtgcagcctt tgaaggtaaa aaacaagatg ttgcattctt agcaacctta ccaacttacg
     5041 aagaagcaat tgcacgttta atgggcacaa tgaaagaagc tgcggcaggc aaacttgttc
     5101 gcactcttgc agcattacgc gatcaaatgg aagcagcagc ttaattttaa gcactcttct
     5161 ttattcgttt aacaacttat tttactttag gaatcgattg ttatgtcatt aactaacgaa
     5221 caaatcattg aagcgattgc ttctaaatca gtatcagaaa tcgttgaatt aatcactgcg
     5281 atggaagaaa aattcggtgt ttcagcagcg gcagcagtag cagcagctcc agcagcaggc
     5341 gcagcggcag cagaagagaa aactgagttt gatgtagtat tagcagaagc tggtgcaaac
     5401 aaagttgctg taatcaaagc agtacgtggt gcaacaggtt taggcttaaa agaagctaaa
     5461 gacttagtag aatcagctcc agctgcatta aaagaaggca tctctaaagc agaagctgaa
     5521 gcacttaaga aagaattaga agaagctggc gcgaaagtag aaatcaaata atttttgatt
     5581 tattttgtca gaacttcaag ttcaacaaac cccgatggaa acatcggggt tttgtttttt
     5641 cagaattgtg taatgtagat atgattacct ttatccatta cttgcgcagc agtgaaaaat
     5701 gtaaaaattc cacaaagtga tctgcccagt gcatttcgtg gtggatttca tttgccataa
     5761 tccgcaggcg gatattgtct aacggcacgc caatccgaat cagtgcttgg tagtaattga
     5821 gcgaacaatc aatataggtt tggttcgtat tagagataaa gtgcgaatcg atttcatcgc
     5881 cttcgttagt gccaacctga ataaacactt tggtattggg ttgtaacgga tattggtgcg
     5941 taaaacgcag aaaatccggc tcactaaacc aagaggcaag cgaaaacacc cctaaatgcc
     6001 cgaaaatatg tgggtagcgg ctgcccatat aagcggtgat aatgccaccc attgaactgc
     6061 ctgccaataa ggtatgttct tgctgaggct tggtgcgata gtgaatatca ataaagggtt
     6121 taaccgtttc caccacccat ttggcatatt cctcccctaa gcctccggca tttcgggttt
     6181 caggggttga gccggtggtt gttttccacg ggccgtattc atttaagcga ttagctcctg
     6241 cgttatcaat gccgacaata atcatcttcg gtaattcttg gtgtgattta atggttggaa
     6301 taattttcca agaatgcccc gaataagact ctttgctgta aaacacattt tgcccgtcgt
     6361 gcatatataa caccggataa cgttcccaac cttcgttgtg ataatccttc ggcaatagta
     6421 cccgaatgcg gcggtgatgg tgtaaatagg gaacatataa aaaatgctcc tgcatttcta
     6481 aatagtggtt aagtggtttt tgtaccatca gtctgtcctt ttgcttaaat tgtggcgtat
     6541 tttacacctt aatcagtctg agttaaaaca agcggtcaga aaaagtcaaa aatttgcaaa
     6601 ttgctgttac ctaacgcaat cggttgcctt atgcttttga ggaatataaa agagaaactg
     6661 tgatctaagt cacagcaatt tttattcggt ttgactaaaa tccgaactat cacttaatag
     6721 agaggaaaat gttatgcagg cttcattaca agatattctt gaggtagtaa aagcaaaaaa
     6781 tctcacttat cagcaaaaat tgatgagttt aggcaatatt gctgagcggt tatttaaccc
     6841 gattgaacta cttggctaca ctgaagaaga gtgggaacac attgaaaatc agatgatttg
     6901 cgatttaaat gaaggttatg cgatttatcg cccacgttat attttgcctg attataatgt
     6961 gtatatgcaa aaaggttgcc aattcttaga tttacctccg ccaaccaatt tagatgaagc
     7021 cttagacggc ttgttaattc tgtattcaca cgttccgtcc attactactt ttccggtgta
     7081 tatcgggcgt ttagaccaac tgttagaacc gtttataacc gatgaagagc aagattacat
     7141 taaaatcaag cgtttcttaa atcatatcga taaaaccatt tccgattctt tctgccacgc
     7201 taacatcggg ccgaatgaca ccaaagccgg tcgtttaatc ttaaaagcgg tgattgagct
     7261 tgaaaacatt acaccgaata tgacgattcg ttatgataaa accaaaacat cgcgtgaatt
     7321 tgccgaactg gcggcgaaag cgtgcttgtt ggtgtccaaa ccgtcttttg ctaatgatgc
     7381 ctactacatt caagatttag gcgaagaata cggaattgca agctgttata acgccttacc
     7441 ggaatgtggc ggtgcttaca ctctgacccg cctgcgttta ggtactatcg ggcgtgcttg
     7501 tgcgagtgtt gaacaaatgg tcaatgagat gctgccgaaa gtggcaaaac tcgccctttc
     7561 cacgatggat aaacggcata aattcttggt ggaagaaagc aatttctttg aaagtagctt
     7621 cttggttcag gagggcttta tcaaacgtac taacttcaca agtatgattg cgattgtagg
     7681 tcttgctgat gcggtgaatc atttactcaa gcaagaaggt ttaaatgaaa cctttgggca
     7741 aagcaaacgt ggcgatgaaa ttgcgacgct gattatggat aagctccaag cagtaaatga
     7801 cgctcataaa gggctgtatg ttgaacgaac caataaccaa tatctgttac acgctcaagt
     7861 gggggcaaat aaccacgctg aagacaaaat gaacacgccg gcacaccgca ttcgtgtggg
     7921 cgaagagcca accttattgg cacacttaaa acagtctgct cctttccata aatatttccc
     7981 atccggtacg ggggatttat tcgcctttga ccaaacctac actgatcatt tagatgcggt
     8041 ggtggatatt attgacggct cgttcgccca aggttatcgc tacattacca cttacctgaa
     8101 aaatactgat ttgattcgag tgacaggcta cttggtgaaa aaaagcgagg ttgaacgtta
     8161 tcgcaaaggc gaagcggtat tgcgtgatac cacttggtat ggttcaggca ccgatgattg
     8221 tgcgaaagtg ttcgaccgcc agctgcgtga tgaagaaaat gtgaatgcag ggtaacaatg
     8281 gctgcacttt ctgagatttt tgtgccgtta caccgcatta tccccttctc gaatgtagaa
     8341 gggcagggca atcgtaccag catttttcta caaggttgca agctgaattg cctgtattgc
     8401 cataacccgg aaaccattcc acgttatagc aaggaagcaa aacaggttag cttgcaatac
     8461 ttgtacgatc aagtgatgga ggcagttccc tttattcgag gagtcactat ttcgggtgga
     8521 gagccaacca ttcatcataa aaaattagtg ccactgttcg aggcgttgcg tgagcaaggg
     8581 ctaacctgtt atttagacag cagcggcttt tttgattttg aggcaacaga gccgttaatt
     8641 aatgttaccg ataaatttct gttcgatctc aaaggagatg gggcgggctt acaaacgcta
     8701 tgctttgatc ggaaaaacca agccggcaaa gtgcctgaac aggctcttcc aaacgtgaat
     8761 catattaaac ctgaaaactt ggctcgcaat ttgcaaaatt tagccaaatt attaccgctt
     8821 gataaggtgg aagaagtacg tttggtttac ctcaatgagt tttataacgc ccaccatttg
     8881 ctcgaaaaag tggctgacct acttaaacct tacccagatg tactacttaa aattattcgg
     8941 gtacacacca aaggagcgag agatgaaaca ggcttggctc gttttgtacc cactatcgag
     9001 caaaccgatg aactggcagc atttgcaaaa cggcacggta ttcaaaaagt tattactatt
     9061 tactaatgga gacttacgat ggatatacta attagcataa ttggtgtttt tgtgttatta
     9121 ggcttaggtg ttttattatc aaataatcgc aaagccatta aatttcgcac aattttgggc
     9181 gctttggcga ttcaaattgg ttttgccgct cttattcttt actttccagc gggaagaaat
     9241 gcattgctag ctactgcaaa ttgtgtgagc aatattatta attatggtaa cgaaggtatt
     9301 agctttgtgt ttggcaattt agcgaatccg agtaatagca gcattggttt tgtgtttgcg
     9361 gttaaagtat tgcctattat tatcttcttc tctgcgttaa tttcaatgct ttactacctt
     9421 ggtgtgatgc aatgggtgat taaaattatt ggtggagcat tacaaaaaac actaggcact
     9481 tccaaggcag aatcaatgtc agcggcagct aattattttt gttggtcaga cagaagctcc
     9541 tctagttgta aaaccataca ttagccgtat gacagagtca gaattatttg ccattatggt
     9601 tggtggaact gcttcaattg ctggttccgt tatggctggt tatgccggaa tgggcgttcc
     9661 tttaacttat cttattgcgg cctcctttat ggctgcacca gctgggttac tatttgctaa
     9721 attaatgtat cctcaaacgg aaaccttgga gagtaaagca gatgacaatg ttgaagttga
     9781 aaagccaaat aacgtggttg aggccctagc taatggtgct agtgcaggtg ttactttggc
     9841 tattaatgtc ggtgctatgt taatagcatt tattgcagtg atcgctttat taaatggctt
     9901 tattggtggc attggtaatt ggtttggtta tgaggggcta accttacaaa ttttattagg
     9961 ttatttattc caaccacttg ctttcttaat cggtgtgccg tggcaagatg ccgatattgc
    10021 aggtcaaatg ataggtatga aacttgctgt taatgaattt gtcggatatc ttgagtttgc
    10081 taaatatcta cagcctgatg ctgtggtgca attaagcgat aaaactaagg caataattac
    10141 ttttgcttta tgtggttttg caaactttag agcaattgcg gtattgatta gtggtatcgg
    10201 aagtatggca ccaagtcggc gttcagatat tgctcgttta ggtttaaaag cagtgattgg
    10261 cggcacattg gcgaacttaa tgagtgcaac tatagcgggt ttctttattg gtttaagtgg
    10321 tgccacatta ggttgataca agcggttaaa ttgactcact tttttgcaaa aagcccaagt
    10381 aagttaaatg actacttggg ctttgtttaa tgaactagct cttagtttcg gtttttgctg
    10441 tgttatcctt ttccgctttg gctgtctgtg ttttcgaagt tgtctgtttt tttgcggatt
    10501 ggctagcagt agctgctttt cttactttct ttgttttaac ttctacttcc gcatttttag
    10561 agtttttatc cgctaaccat gcctgcactt tagctaagaa tatttgtccc atcgggcgag
    10621 gatcgccaga aaataatgtt tgtaatttat tattttcaat aatgtcgcgt ctaattgcta
    10681 agcgttcttc gtggttttcc gctaaacgaa tagcttgagt aacgtattcc tctgctgttt
    10741 gggtaattaa ccaattaggt agcccgaggc gcttaaataa accttcatca atatgttcat
    10801 gaacttcagg gcctgtttta cagacaccaa ctaaaccaag cgtaaccata tcgataatgc
    10861 cgtttgtatt tccaaatggg aatggattta gcatcatatc gcaattatgt agaatattta
    10921 gatattcatc atatggcgaa tgtgggtgtg cggttgcatc atttcctaaa tagcttttga
    10981 taaaacgttc cacataagga tgcgtaattc cactagattg ccctaatgca aaatggaaat
    11041 gagtttttac cttcgcccta tcacgaatgg cttttaatgc ctctaagaaa tatgggttta
    11101 atttcatagt ggttgatgca attccgatat ggataacctc tggattttca cgtaggttat
    11161 agaccacatt ttgaggcgct aatgcggaag gcacataggg tagcgcatct tttggaagac
    11221 gtaataattg ttcactaaag cagctttcgg aaccaacata gtcatcttct acgatgacat
    11281 attcaataaa atcagagtgt gtagttgccg gatgacctag cgcaacagct tgaattggtg
    11341 ctaggcgagt gttactagcg aaaatagtgg ttaaatccat tccaatgcta ggcatataga
    11401 atacggctgg attattttta tcacagatat cttttaaaaa gcttaagcgg tcaaatagtg
    11461 agccatcttg tggtaataaa tgaaattcgt cgaatacctg ttgccccatt tcatctacag
    11521 catcactgcc tagcccaata agatggaaat gttcgcgtgc agcaaccatt gaggttgaat
    11581 gagtacgata aatagaatgt gatgaatgga aatgctccaa taatactacc atgacaggtt
    11641 tattatttct ggtaccaagc tgttcaattt ttctatcttc ccaaccgaca gaaagtaaat
    11701 gacgacgaat tacagcattt aacgaccgtt ttacattatg tttatttgct tcaatatcat
    11761 aactgcaatg catataaaca tcgtgtgaaa tggcgctcgg tagcttgttc aagttttcaa
    11821 tttgcgctaa tttttctggg aaccattgca aaatagccgc gcgttttgca aaagcagagg
    11881 atgtaccaat aaatctaggt gattggagtg caaaacaaag tgatgcggtg agatctgatg
    11941 aagcattcca tgcagcatct agattcaata aaatattaga ttccggtaag tataaaatac
    12001 agaacttaat tagtgcttgt aatgtggcat ctaaatcaac cgtatttgga ttagcagatt
    12061 ctttatttct attgtaagta cgcaagatat ggtctgcgtt tacgtatgga gaactggcaa
    12121 aaattaatgc tatccagcgt tgtaatgtca gaaaacgcat agcaccgtgt tcggagatct
    12181 ctaatgcagg atcttcaaac aagcgagtaa tcgctaccgc cattcgagta cagaaataga
    12241 tagctttatc attttctaaa ttttcaatct gctctggcat attcaattca atattggaaa
    12301 tgccaccaaa attactatct aattttgaaa ggatagacag tagctcggta caagccgatt
    12361 cataatcttt tttcgcgacc gcctgctcaa aacggatgac gctaggcata ttttctgctg
    12421 acataatgta tccttagttg tttaaaattc cccacagatc atactcatct gcatgtgtaa
    12481 tggttactga aataatttgc cctactgtca ctaattggtt tgaaggatta tctacataga
    12541 ctacaccgtc aatttccggt gcatctgcca ttgagcgacc gataatgcct tcttcgtcaa
    12601 tgtcatcaat aattacttgt acggttttgc cgatttttgc ttgtaagcga gcagcggaaa
    12661 tttcttgttg tagttgcata aaacggtgga aacgttcctc ttttacttct tccggaactt
    12721 gatccggcat atcggtagca acagcacctt ccactgggct gaatttaaag caaccaacac
    12781 gatctaattg tgcttctttt aagaaatcga gtaatagttg gaaatcttct tctgtttccc
    12841 ctgggaagcc gacaataaag gtagaacgca gagttaattc agggcaaatt tcacgccatt
    12901 ttttaatgcg ttctaaggtt ctttcgattg aacccgggcg tttcatcgct tttaagattt
    12961 tcgggctggc gtgttggagt ggaatatcca aatacggcaa aattttgccg gcagccataa
    13021 gcggaattaa atcatctacg tgtgggtatg ggtaaacata gtgcaaacgc acccacacgc
    13081 ccattgagcc taattgctca catagcgtaa ttaaattact tttaatcggc ataccgttcc
    13141 agaaaacggt tttggttgct tcttcttttt tgcgatctaa cgaataggct gaggtgtctt
    13201 gtgagacgat taacagctct tttaccccgg catcaactag gcgttttgct tcatctaata
    13261 cttgcacgat agaacggctt gctaaatcgc cacgcattga aggaataatg cagaaagtgc
    13321 agcgatgatc gcagccttcg gaaattttca aataagcgta atgcttcggt gttaatttaa
    13381 tcccttgttt tggcactaag ctggtgtaag ggttaaattc cggttttggc acatatttat
    13441 ggacgtggtt cattaccgcc tcatagctat gtggccctgt aatttccaat actttagggt
    13501 gtacctcacg aatttggttt tcttttgccc ctaaacagcc ggtgacaatt acttttccgt
    13561 tagcttctaa cgcttcacca atgctttcaa gagattcttg tactgcggaa tcaataaaac
    13621 cgcaggtgtt tacaatcaca agatccgcat tttcataact agggatgatg ttataaccat
    13681 ccgcacgcag ttcggtcaaa atacgttcgg aatcaactaa atttttaggg cagcctaagc
    13741 tgataaaacc aatatttggt gaagcactca tataactctc aataactaaa aagaaaaatg
    13801 cctacaattt ttgtctgtag gcaaaaaact aacaagattc tacaaaatct tgcccaaaat
    13861 agcgataaca agcggtcaat ttttaataaa catttacaaa cttgaaattt cgctaacaat
    13921 taaaattaat cgatattatt tagcattgct aatacaatcg tggaagattt ttctgcggct
    13981 aatggcagaa attcttcaaa agaaaggtgg gattctttgt ccgctacatc cgatactgct
    14041 cttacgatta caaatggaag gttaaaaccg tgacaaacct gcgcaatggc tgctgcttcc
    14101 atttcgactg caacgacatt cgggaaattc tgacgaattt gattaatctt atcattaccg
    14161 ttaataaagg catcgccgct acagattaag cctgaaacca cattgtaacc ggcttttttc
    14221 gcttcctttt gagctaattc gactaattgc tcgtttggcg aaaatgttgg cgggtttgcc
    14281 ggtagttgcc cgatttcata accaaaggcg gtcacatcaa catcgtgata acgaacttcg
    14341 gttgaaatca aaatatcacc tacgtttaaa tctggatcta atccaccggc agagcctgta
    14401 ttaataatca tatccggctg ggtgagctgt atcagtaagg tagtgccgat ggcggcagca
    14461 actttgccga tacccgattg taataatgcc acacgggtat tgttaatttt gccttcaaaa
    14521 attttacaac ctgctaattc agtgacttta ggttcaacca ttacttggcg taaaatctca
    14581 atttcttgtg ccatcgcacc aataatacca attttcatta ctttccttct ctaatgtatt
    14641 gtttattttt tgaaaaaaac gctattttaa cagcattaat agtgaggagg gggtgtttct
    14701 tctgcttgtg aagcaacttg gcttagttga gcccctttca atttctcagc aatatggcga
    14761 atctgagttt gtaacttatc aattgcaaat tgttgctcaa taagtgcttg atttaactct
    14821 tcaataatca gctcttggaa agcaacttta gtctccaatt cggtaatttt tgccgataat
    14881 gtataattat tatccatttt ttactaaaaa aaggttgtgt gaaaaggaat tacactttac
    14941 cctatcttaa attttcttaa cagttatttt tataaggaaa aattatgtta
//
LOCUS       DANLXS010000046        14866 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.046, whole genome shotgun sequence.
ACCESSION   DANLXS010000046 DANLXS010000000
VERSION     DANLXS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14866
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.046"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..262)
                     /locus_tag="PXD71_001028"
     CDS             complement(<1..262)
                     /locus_tag="PXD71_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291165.1"
                     /translation="MGIKVLFGTKISAELQYLPEKDLVKILQFKQHVEIYGLENLAGR
                     NKSSDDVPTNDPYWAEKVAKAQKYSLWHYHIGIPEYDTSQGFG"
     gene            complement(252..725)
                     /locus_tag="PXD71_001029"
     CDS             complement(252..725)
                     /locus_tag="PXD71_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291166.1"
                     /translation="MMKNLPLSMADTLAVIYPAFDFAADDKSATEQQISLLNFRITMA
                     NQLLIACIETIEKNPNFWAEIDLNDFKKYLNQNKVVIFDTQQYFTDGSRTMAILDNMK
                     SNIRKIEQQLSQATAFNFDLERMKERLNDTFHEVPKDITNVNEFRNWLKGVANGH"
     gene            complement(793..1881)
                     /gene="rlmM"
                     /locus_tag="PXD71_001030"
     CDS             complement(793..1881)
                     /gene="rlmM"
                     /locus_tag="PXD71_001030"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDL6291167.1"
                     /translation="MFNKLALYCRAGFEKEVAGEITDKAAQQGIFGFANLKENSGYVI
                     FECYQAGEADRLAQALKFEQLIFVRQMLVVGDLLQNLSENDRISPILAQYAELNPRQS
                     SDVFVETPDTNEAKELLTFCRKFTVPLRSALKKQGWLGKSENMQGSVSLHILFTRPGA
                     CYVGYAYNHNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPQAEEKKRFNDTMVG
                     VDLGACPGGWTYQLVKRGVFVYAVDHGKMAASLHETGRIEHCPEDGFKFQPPKRKQID
                     WLVCDMVEQPIRISKLIAKWLTNGWCRETIFNLKLPMKKRYQEVQLCLNFLEEELTKH
                     GFWFKMQAKHLYHDREEITVHIAVMGQK"
     gene            complement(1881..2273)
                     /locus_tag="PXD71_001031"
     CDS             complement(1881..2273)
                     /locus_tag="PXD71_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL6291168.1"
                     /translation="MKNKFVAIAAFSGLFTVAFGAIASHALSAQLSPKALGWIETGIK
                     YQMFHTLAILALGLFQIANFGQNPPVCRAKAFNIIGSNWALGILLFSGNLYLLALNVP
                     TIHWLTPIGGLCFMLGWIALIYISVRKA"
     gene            complement(2380..3781)
                     /locus_tag="PXD71_001032"
                     /pseudo
     CDS             complement(2380..3781)
                     /locus_tag="PXD71_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
     gene            complement(3936..6293)
                     /locus_tag="PXD71_001033"
     CDS             complement(3936..6293)
                     /locus_tag="PXD71_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HDL6291169.1"
                     /translation="MNTALEKGKFVFLDKPKNTITTAAHWGALNVTVENGKVVKSEGS
                     LAKNHQNELQDVVADQLYSPVRVKYPMVRKGYLEGNKDTTLRGRDKWVRISWDKAFEL
                     VSGEIQRVREQYGSESIFGGSYGWYSSGSLHASRTLLQRYLNVTGGFTNTKGDYSTGA
                     VQVIMPYVLGNIEVYAQQTSWHTIVESTELLVIWSSNPLNTLRIAWTSAEPEGIDYLA
                     KLKASGKRIICIDPVKSETCQFLEAEWLPINTGTDVALILGIAHTLITENKHDHDFLT
                     KYTVGFEQFADYVLGKADNLPKTAEWASQICDIPEEVIKTLANDFVTHRTMLMSGWGL
                     QRLQHGEQSHWALVTLAAMLGQIGLPGGGFGLNYRYSTNGLNVNSGVRLGAINAGKPN
                     PDMPFFPVSRIADALLKPNQPYDYNGQTLHYPNIKLVYWAGGNPFAHHPDTNYLVKAW
                     QQPETMIVNECYWTPTARMADIVLPATTSYERNDLTVAGDYVAKHIIPMKQVVEPQFE
                     AKNDYDIFAELAKRAGKEQAFTEGKSEMEWLKEFYQVAFEAGEKANTPLLDFEAFWAE
                     NKVLSFEAEHQNNTFVRYQDYRTDPIKHKLATPSGKIEIFSETIAKMNYEDCKGHPTW
                     FEHHEYSGKTNEAEPLALVTPHIKYRLHSQLAYSSLRQYYEVNGREPMLIHTKDAADR
                     GITSGDIVRIFNKRGQVLAGAVVTDGIIQGTVALYEGGWYDPQDLGKTDTPLCKNGNP
                     NVLTRNEGSSKLGQGNAPNTTTVQVEKYVGDAPEVTVFKAPFFSE"
     gene            complement(6429..9035)
                     /gene="acnB"
                     /locus_tag="PXD71_001034"
     CDS             complement(6429..9035)
                     /gene="acnB"
                     /locus_tag="PXD71_001034"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDL6291170.1"
                     /translation="MENFIENYQQQVDERAALGVVPQPLSAEQTAQLVELLKNPPAEQ
                     AVFLLDLFTNRIPAGVDEAAYVKAAFLADITKGVATSPLISPAHAVKLLGTMQGGYNI
                     ETLLSALDNPELAPIAVEALSKTLLMFDNFHDVKERADQGNPFAQQVLESWANADWFT
                     SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNERDGIFPDENG
                     HTGPIKQLEALKEKGFPLAYVGDVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVV
                     LGGKIAPIFFNTLEDAGSLPIEVDVTNLNMGDVIDIYPYAGKICKHNSDEVLAEFELK
                     TEVLLDEVRAGGRIPLIIGRGLTHKARVALGLPESDVFIKPQSVSESNKGYTLAQKMV
                     GRACGVEGVRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPVDVITHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGTMQPGITLRDLVHAIPYYAIQQGLL
                     TVEKKGKKNIFSGRILEIEGLEDLKIEQAFELSDASAERSAAACTIKLNKEPIIEYLN
                     SNITLLKWMIAEGYDDKRTLERRIANMQAWLDNPELLEADENAEYAAVIEINLDEIKE
                     PIVCVPNDPDDARLLSQVQGDKIDEVFIGSCMTNIGHFRAAGKLLSQAQGELPTKLWI
                     APPTKMDAGLLTEEGYYSIYGKSGARVEMPGCSLCMGNQARVANNANVVSTSTRNFPN
                     RLGQGANVYLASAELAAVSALLGKLPTPQEYLDYMNNLQKDKDDIYRYMNFDQINSYV
                     KKADNVIVQVAV"
     gene            complement(9055..10302)
                     /gene="icd"
                     /locus_tag="PXD71_001035"
     CDS             complement(9055..10302)
                     /gene="icd"
                     /locus_tag="PXD71_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDL6291171.1"
                     /translation="MQTQVQIPQGETIQLNQDGSLQVPDNPIIPFIEGDGIGVDVTPA
                     MKAVLDAAVEKAYQGKRKIAWMEIYAGEKANKLYGENTWLPDETLELIRQYHVAIKGP
                     LMTPVGGGIRSLNVAMRQGLDLYNCLRPIRYYDGTPSPVKHPELVDMVIFRENSEDIY
                     AGVEWVAGSPEANKVIEFLQQEMGVKKIRFTEDCGIGIKPVSKQGTQRLVRAALQYVI
                     DNNRKSLTLVHKGNIMKFTEGAFKEWGYQVAEEFGAKLIDKGPWMSLTNPKTGEEIII
                     KDSIADAFLQEILLHPTEYDVIATLNLNGDYISDALAAQVGGIGISPGANIGFEAAIF
                     EATHGTAPKIAGQNKGNPGSLILSGEMMLRHLGWVEAADLVVKAVSKTIANKTVTFDF
                     AEMLEGVTLRSTSEFAQDIIANM"
     gene            10536..11816
                     /locus_tag="PXD71_001036"
     CDS             10536..11816
                     /locus_tag="PXD71_001036"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005762304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDL6291172.1"
                     /translation="MSKATLTLETGEKLELNMQKGTLGYQTIDIQPFLKHKLFAYDPG
                     LVSTAVCRSNITYVDGDEGVLLYRGYPIDQLAKHSDYLEVGYMLLFGERPTKEEYKDF
                     VQLIKKHTLVHEQLTKFFSGFRRDSHPMAVMCGVSGALAAFYHDAIDVNNQAHRELTA
                     IRLLAKIPTLAAMCYKYSIGQPFMFPQNHLSYAGNFLYMMFATPCEPYEVNPVLERAL
                     DRIFILHADHEQNASTSTVRTAASSGANPFACIAAGIASLWGPAHGGANEACINMLKE
                     IGTVDRIPEFIARAKDKNDPFRLMGFGHRVYKNYDPRAKVMRETCHEVLKELNIDNPL
                     FEVALELERIALSDPYFIEHKLYPNVDFYSGIVLKAIGIPTSMFTVMFALARTVGWIA
                     HWKEMYLQGNIKIVRPRQIYTGHTKRDFEPMDKS"
     gene            complement(11889..13118)
                     /locus_tag="PXD71_001037"
     CDS             complement(11889..13118)
                     /locus_tag="PXD71_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291173.1"
                     /translation="MFQAEPKNGKVTVEVLKQYLIDELKIDEEEIAIVIGNQHELDNI
                     DLFNPQCKINYIITVEALKEGWDCPFAYIFCSVQNVSSSKEAEQLLGRVLRMPYAKHR
                     QIEDLNRAYAPLSSKSFGETARNLQDKLIGMGFEALEVAEMLRIPQQENWIGNEGDLF
                     YTPTTVLALTQLPEMSDLSEAKRSQLKITEQEGHILVQVKGNLSEKLEKALIRTATGK
                     KKEQIEQQLQIHQNRVEILSSPAEKGEIFKVIPQLCMNIQGEFDLADSQVLLDYQSWN
                     LLDYPAKLDGFRLQENSQSFEIDVEDKRVSYHFNQQQLSFSDDWLRNPVKRGFSLPLA
                     HQNFYPDFIVELIDGRILIVEYKGEQLKSNEDSKEKALIGNLWEKLSQGKGLFIMAVK
                     SDENGQDVRTQLLNKLA"
     gene            complement(13085..14074)
                     /locus_tag="PXD71_001038"
     CDS             complement(13085..14074)
                     /locus_tag="PXD71_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL6291174.1"
                     /translation="MKLKNYQNEALNRVSHYFSACLQSDAKTAFSQIQPEYSYKIPSE
                     HSNLRDVPYVCVRIPTGGGKTLLASHSIARIAKQYLDCDFPVTLWPVPSRTIKTQTTE
                     ALKNPQHPYRAVLDKTFNREVMVIESEDFDLLRPQDFGNKAIVIVSTIQNFRVENQDG
                     RKIYAFNEKLTAHFERLLPHIQANLEKISTTDLQENGLTSKQTGKIKCSFANLLRAYQ
                     PLVIVDEAHNARTSLSFDVLSQLSPSAILEFTATPNTDRKAGSNVLYHVSASHLKAEE
                     MIKLPIVLTEHHNWQQAIDGAVINRGALAQKAQYESEYVRPIYCFKLSRKTAK"
     gene            complement(14090..14608)
                     /locus_tag="PXD71_001039"
     CDS             complement(14090..14608)
                     /locus_tag="PXD71_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6291175.1"
                     /translation="MNRRYIGIEIGEHAKTHVVPRLKKVIEGEQGGISKAVNWQGGGS
                     FRFCELGEEVFDAFGSLNPNIRFDDLAAHIWYLENHFPLQKNSEKSPLVGTFNGKAYY
                     LLYNGILGDKRPQSGNVLTRKLMAELPYFAEFIQQGMDIVVYGEACRLGEVQLAEKKI
                     TFKQIPYDVMSR"
     gene            complement(14618..14689)
                     /locus_tag="PXD71_001040"
                     /pseudo
     CDS             complement(14618..14689)
                     /locus_tag="PXD71_001040"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14696..>14866)
                     /locus_tag="PXD71_001041"
     CDS             complement(14696..>14866)
                     /locus_tag="PXD71_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6291176.1"
                     /translation="RRVFERSAEKKKIHNLISRAHYDMATNEDSASEHLELFGEDIAE
                     DSFDYPKPEKLI"
BASE COUNT         4258 a         3362 c         2817 g         4429 t
ORIGIN      
        1 taccaaatcc ttgactggta tcatattctg gaatgccgat atgataatgc cataaagaat
       61 atttttgtgc tttagccacc ttctctgccc aataaggatc attcgttggc acatcatcag
      121 aacttttatt tcgaccagct agattttcca atccataaat ttcaacgtgt tgtttaaact
      181 gcaggatttt gactagatct ttttcaggca aatattgcaa ttcggctgaa atttttgtac
      241 caaataaaac tttaatgccc atttgcaaca cctttcaacc agtttctaaa ttcattaaca
      301 ttcgttatat ctttaggcac ttcgtgaaaa gtatcattta accgctcttt cattcgctct
      361 aagtcaaaat taaatgcggt tgcttgagag agttgctgtt caattttgcg aatattggac
      421 ttcatattat ccaatatagc cattgtcctt gagccatcag taaaatactg ttgggtatcg
      481 aaaatcacca ccttattttg atttaggtat tttttgaaat cgtttaaatc aatttcagcc
      541 caaaagtttg gatttttttc gatagtttca atacaggcaa ttaataattg atttgccatt
      601 gtaatacgaa aatttaacaa tgagatttgc tgctctgttg cagatttatc atcagctgcg
      661 aaatcaaatg ccggataaat cactgcaagc gtgtcagcca tagataaggg taaatttttc
      721 atcatctctc cttacggaag aagttgtcat tatctactgg attacttaaa aaaacaactt
      781 attcatttta tgctattttt gccccatcac cgcaatatgt accgtaattt cctcacggtc
      841 gtggtagagg tgtttagctt gcattttgaa ccaaaacccg tgtttcgtta gctcttcttc
      901 caagaaattg aggcatagtt gcacttcttg ataacgtttt ttcatcggca gttttaggtt
      961 gaaaatcgtt tctcgacacc aaccgttagt cagccatttt gcaatgagtt tcgagatacg
     1021 aatcggctgc tctaccatat cacaaaccag ccaatcgatt tgtttacgct taggcggttg
     1081 gaatttaaac ccatcttcag ggcaatgctc aattcttccg gtttcgtgta aacttgctgc
     1141 cattttgccg tgatcgactg catagacaaa aacaccacgt ttgactaatt gataagtcca
     1201 accacccgga caagccccaa gatcgacccc taccatcgta tcattaaagc gttttttctc
     1261 ctctgcttgc ggaataaaag ttaaaatcgc ctcttctaat ttaagcgttg agcgactagg
     1321 tgcctcagcc gggaatttta aacggggaat ccccataaaa aacggcgatt tattatggtt
     1381 gtacgcatag cccacatagc aagctcccgg acgagtaaat aaaatatgca agctcacact
     1441 accctgcata ttttccgatt tccccaacca accttgtttt ttcaatgccg accgtaatgg
     1501 cacagtaaat ttacgacaga aagttaataa ttctttcgct tcgttggtat ccggtgtttc
     1561 gacaaataca tcagaacttt ggcgaggatt aagttcggca tattgggcta aaatcggcga
     1621 aattcgatca ttttcagaca gattttgcaa caaatcccca acaaccaaca tttggcgaac
     1681 aaaaataagc tgttcaaact ttaacgcttg ggctaatcga tctgcttccc ctgcttgata
     1741 gcactcaaaa atcacatagc cactattttc tttaaggttg gcaaagccaa aaatgccctg
     1801 ttgtgcagct ttatccgtga tttcgcctgc tacctctttt tcaaaccctg ctcggcaata
     1861 taaggctaat ttattgaaca ttaggctttt ctcacactga tataaattaa cgcaatccaa
     1921 ccaagcataa agcagagacc accaataggc gtaagccaat gaatagtcgg cacatttaac
     1981 gccagtaagt acaggttgcc actaaaaagc agaatgccta acgcccaatt gcttccgatg
     2041 atgttaaagg ctttggctct acaaacgggt ggattttgcc caaaatttgc aatttggaat
     2101 aaacctaaag ctaaaatggc aagggtgtga aacatttgat atttaatccc cgtctcaatc
     2161 cagcctaagg ctttaggcga taattgagcc gataatgcgt ggctggctat cgccccaaat
     2221 gccacagtaa ataagccgct gaatgcggca attgctacaa atttgttttt cataagttga
     2281 agataggtat gatgttagat ggcttactat atcatattat cgaaaccaac aagcggtgaa
     2341 attttgcaaa aaaattacaa aaaatcaccg tttgtatgct taatggttca agataatatc
     2401 cacatcttcc gatttatcgt gaaccccaaa tctatctact aaggttttgc tggcctcatt
     2461 taatccgata atttctacct caacatcact ttttcgatat ttcaacacaa ctttatctaa
     2521 cgctgaaact gccgttacgt cccaaaagtg agcgtgggtt aaatcaatgt ggactttttt
     2581 cagttctgaa cgggtaaaat caaattgtgc gataaaatca tcagatgagg caaaaaatac
     2641 ttgcccgata acatcataac gcaattcctc tttctctata gcacttgttt taaccgccat
     2701 ataacgtcca attttattcg caaagaataa tgccgagagc aacacgccgg aaaatacacc
     2761 aagggctaag ttatgtgtat aaaccacaat tgcaacagtg actaacatca ctacattaaa
     2821 gccgattggg tgcgttttta gctcttttaa ggaatgccaa ttaaaggtgc cgatagagac
     2881 cataatcatc actgcaacta atgcagccat cggaatcact ctcaaaatat cgctcaaaaa
     2941 gaccacaaaa atcagtaata atattggcaa tgccctgccc tttacattca cggtttttat
     3001 cgctgtctgt ctctgtcatt tcatcaacaa tcgttgccgt cattaaagat tccaataacc
     3061 ctactgcggc caagctgaaa gaataaggca aaataatcca taaggtgtct aaattaagcg
     3121 gaatatcggg taataaaaac accggtaagg tatcgggtaa ttgccccata tccccaacgg
     3181 tacgaacatc aatgccttgc cataatgtga aaagtgttaa agcaataatg gtcactaatg
     3241 gtgagggaat gatttttcca atcaccggta cataaggaaa aagataaatg atagcaagcc
     3301 ctgctaccac cagtgcataa gtatgccaag ttgtattttg taactcaggt agttgtgcca
     3361 taaaaattaa aatggcaagt gcattaacaa acccaatcac tacggctcgt gccacaaacc
     3421 gcataagcga gccgagtttt aaccaaccgg caataatttg caacacgcct gttaataagg
     3481 tggccgctag catatattgt aaaccgtgat ccctcactaa gcctgctaat accagagcca
     3541 ttgcaccggt tgctgcagaa atcatcgccg gtctgccgcc cacaaaactg ataattactg
     3601 caatacaaaa tgaggcatat aaaccaattt gtggatcaac ccctgcaata attgaaaatg
     3661 ctatggcttc tggaattaac gccaaagcca ccactaaacc agagagaaca tcacctcgta
     3721 catttgaaaa ccattgttgt ttaattaccg taggcgaaaa ttgctgaaat aaagttgtca
     3781 ttattaccct taaataaaaa gaaaagccga gcaaagtcgg ctaaaaatga ggcgagagag
     3841 tagcaaatta gtgaaaagtc gtcaaataaa caagcggtct tctttcgctc tttttttgca
     3901 aaaaatagta gaaaaatgac cgcttggatt aattgctatt cactaaaaaa aggggcttta
     3961 aacaccgtca cttcaggtgc atctcccaca tatttttcca cttgaaccgt ggtagtgttc
     4021 ggtgcattgc cctgcccgag ttttgatgaa ccctcattac gggttagcac attcgggttg
     4081 ccgtttttgc atagtggagt atctgttttg cctaaatctt gcgggtcata ccaaccacct
     4141 tcgtataacg ctactgtgcc ttgaatgatg ccatcagtca ccactgctcc tgccaacacc
     4201 tgccctcgct tgttaaaaat tcgcacaata tcaccgcttg taatgcctct gtcagcggca
     4261 tcttttgtgt gaattagcat tggttctctg ccgtttacct cgtaatattg acgtaatgag
     4321 gagtaagcaa gctggctgtg taaacggtat ttaatatgcg gtgtaactaa tgccaacggc
     4381 tcggcttcgt tggttttgcc tgaatattcg tgatgttcaa accaagtcgg gtgtccttta
     4441 caatcttcat aattcatctt ggcaatggtt tcggagaaaa tctcaatttt gcctgatggc
     4501 gttgccaatt tgtgtttaat cggatcagta cgataatctt gatagcgaac gaaagtatta
     4561 ttttgatgtt ctgcctcaaa gctcaacact ttattttccg cccaaaatgc ctcgaaatcc
     4621 agcaatggcg tattagcttt ttcccccgcc tcaaaggcaa cttggtaaaa ttccttaagc
     4681 cattccattt cgcttttgcc ttcggtaaat gcttgttctt taccggctcg tttcgctaat
     4741 tcggcaaaaa tatcgtaatc attcttcgcc tcaaattgcg gttcaaccac ctgtttcatc
     4801 gggataatat gttttgctac ataatcaccg gcaacggtga gatcgtttcg ctcgtagctg
     4861 gtggttgcgg gcaatacaat atctgccatt cgagcggtcg gtgtccagta acattcattg
     4921 acgatcatcg tttccggctg ttgccacgct ttcactaaat agttggtatc cgggtggtga
     4981 gcaaacggat taccccccgc ccaatagact aatttgatgt tcgggtaatg taatgtttgc
     5041 ccgttgtaat cataaggctg attcggtttg agcaacgcat cggcaattcg agacaccggg
     5101 aaaaacggca tatcggggtt aggtttacct gcattgattg cccctaaacg aacaccggaa
     5161 ttaacattta gcccattggt agaataacga taatttaagc cgaaaccgcc gcccggcaag
     5221 ccaatttgcc cgagcatagc ggcaagagtc actaacgccc agtggctttg ctccccgtgt
     5281 tgcaggcgtt gcaagcccca gccggacatc aacattgtgc ggtgagtaac aaaatcattt
     5341 gctaaggttt taatcacctc ctccggaatg tcacaaattt ggctcgccca ctctgctgtt
     5401 ttaggtaaat tatctgcttt tcctaacaca taatcagcaa attgctcgaa accaacagta
     5461 tatttagtta agaagtcgtg atcgtgcttg ttttcagtaa taagggtgtg ggcgatccct
     5521 aaaatcagtg caacatccgt gccggtatta ataggcaacc actctgcctc taaaaattga
     5581 caggtctcgc ttttcaccgg gtcgatacaa ataatccgtt taccgcttgc ttttaatttg
     5641 gcgagataat caatcccctc cggttcggca gaggtccacg caatgcgaag tgtattcagt
     5701 ggattagacg accaaattac caataattct gtgctttcta caattgtgtg ccaactggtt
     5761 tgttgagcat acacctcaat attgcctaat acataaggca taatcacttg caccgcaccg
     5821 gtggaataat cgcctttagt attggtaaag ccgcctgtga cgtttaaata acgttgcaaa
     5881 agcgtgcgag aggcgtgcag tgaaccactg ctataccagc cgtaagaacc gccgaaaatg
     5941 gattcgctgc catattgctc tcttacccgt tggatttcgc cggaaaccag ctcaaaggct
     6001 ttgtcccaag agattctcac ccacttatct cgccctcgca aagtggtgtc tttattccct
     6061 tccaagtagc ctttccgcac catcggatat ttcacccgaa ccgggctata gagctgatcc
     6121 gccaccacat cttgcaattc gttttggtga tttttcgcta aactgccctc tgatttcacc
     6181 acttttccgt tttccaccgt tacatttaat gcaccccagt gggcggcggt ggtaatggta
     6241 tttttcggtt tatccaaaaa gacaaacttc cctttttcta atgctgtgtt catttaaatt
     6301 aatgcctttt ctgttacaaa attgagagga atttactgga ttaagccaaa agttgcaacc
     6361 acaagcggtt gtttttagcc aaaaatttgc aaaacaaaac ccacctaaaa aggtgggctt
     6421 caaggaagtt aaacggcaac ttgaacgatt acattgtccg cttttttcac ataactattg
     6481 atttggtcaa agttcatata gcggtaaata tcgtccttat ctttctgcaa gttattcata
     6541 tagtctaggt actcttgcgg tgtcggcaat ttacccaata acgcagaaac tgcggctaat
     6601 tctgctgagg ctaaatacac atttgcccct tgccctaaac ggttcgggaa gtttcgggta
     6661 gaggtagaaa ccacattagc attatttgcc actcgggctt ggttacccat acagagagaa
     6721 cagcccggca tctctaccct tgctccgctt ttgccgtaaa tgctgtaata gccctcttcg
     6781 gtcagcaagc ctgcgtccat tttagtcggc ggagcaatcc ataatttagt cggcaattcg
     6841 ccctgtgctt ggcttaacag tttacctgcc gcacggaaat gcccgatatt agtcatacaa
     6901 gaaccaataa agacttcatc aattttatcg ccctgtactt gagaaagcag gcgagcatcg
     6961 tcagggtcat tcggtacgca cacaatcggc tctttgattt catctaagtt gatctcaatc
     7021 actgcggcat actccgcatt ttcgtctgcc tctaaaagct ccggattatc caaccacgct
     7081 tgcatattgg cgatacggcg ttctagcgtg cgtttatcgt catagccttc cgcaatcatc
     7141 cattttaaaa gcgtaatatt agaattcaga tactcgataa tcggctcttt gttgagcttg
     7201 atggtacagg ctgcagcaga gcgttcagca gaagcatcag aaagctcgaa ggcttgttcg
     7261 attttcagat cttccaaccc ttcaatttct aaaattcgac cggagaaaat atttttctta
     7321 ccttttttct ccaccgtcag caagccttgc tgaatggcat aatacggaat agcgtgaacc
     7381 agatcccgta aggtaatgcc cggttgcatt gtgccggaaa aacgcaccaa gaccgattcc
     7441 ggcatatcta acggcatcac ccctgttgcc gcagcaaagg caactaagcc cgaaccggca
     7501 gggaatgaaa tcccgattgg gaaacgggtg tgggaatcgc cgcctgtacc gacagtatcc
     7561 ggcaataaca tacggtttag ccaagagtga atcacgccat cacccgggcg aagtgacaca
     7621 ccgccacggt tcataataaa atcaggcaaa gtgtggtggg taattacgtc caccggtttc
     7681 gggtaagcgg cagtatggca gaacgattgc atcacaagat ctgccgagaa gcctaaacag
     7741 gctaaatctt tcagctcatc tcttgtcatt ggtcctgtgg tatcttgtga accgacagac
     7801 gtcattcgag gctcgcaata ttgccccggt cgaacgcctt ctacgccaca agctcgccct
     7861 accatttttt gagcaagggt gtagcctttg ttactctctg aaaccgactg cggtttaata
     7921 aatacatcac tttcaggcaa gccaagtgct actctggctt tatgggtcaa accacgtccg
     7981 ataatcagcg gaattcgccc gcctgcacgc acttcatcaa gcaatacttc ggtttttagc
     8041 tcaaattcgg ctaagacttc atcggagttg tgtttgcaaa ttttgcctgc ataagggtaa
     8101 atgtcgatta catcgcccat attcaggttg gttacatcca cttcgatcgg taaagaaccg
     8161 gcatcttcta atgtgttaaa gaaaatcggg gcaattttac cgcctaacac caccccaccg
     8221 gcacgtttgt ttggaatgta aggaatatct tcccccataa accaaagcac ggagttggtt
     8281 gcggatttac gagaagagcc ggtgccgact acatcaccga cataggctaa cgggaaacct
     8341 ttttctttta atgcttccag ttgcttaatc gggccggtat gaccgttttc atcggggaaa
     8401 ataccgtcac gttcattttt cagcattgct aatgcgtgaa gtggaatatc agggcgagac
     8461 caagcgtctt gagccgggga aagatcatcg gtattggttt cgcctgtgac tttaaatacc
     8521 gttaccgtta atttttcggc aagtttcggg cgagaggtaa accaatcggc attcgcccaa
     8581 gattccaata cttgttgtgc aaacggatta ccctgatctg cccgttcttt tacatcgtgg
     8641 aaattatcaa acatcaataa tgttttagat aaggcttcta ccgcaatcgg tgcaagctca
     8701 gggttatcca acgcagacaa taaggtttca atgttataac caccttgcat tgtgccgagt
     8761 aatttcactg cgtgagcagg cgaaatgagc ggcgatgtgg caaccccttt ggtaatatcg
     8821 gctaaaaagg cggctttaac ataagcggct tcatcaacac ctgccggaat acgattggta
     8881 aaaagatcca ataaaaagac cgcttgttca gccggtgggt ttttcaataa ttcaaccagt
     8941 tgagccgttt gttccgcaga aagtggctga ggcacaacac ccagtgccgc acgttcatca
     9001 acctgttgtt gataattttc tataaaattt tccatcatca ttccctctcc aactttacat
     9061 attggcaata atgtcttgtg caaattctga ggttgagcgt agagttaccc cctccagcat
     9121 ttccgcaaaa tcaaaggtga cagttttatt ggcaatggtt ttagacaccg cttttaccac
     9181 taaatcagcc gcttcaaccc aacctaaatg gcgtaacatc atttccccac ttaaaattaa
     9241 tgacccagga ttgcctttat tttgccctgc aattttaggg gctgtgccgt gtgtggcttc
     9301 aaaaatagcc gcttcaaacc caatattcgc tcccggtgaa ataccgattc cgcctacttg
     9361 tgcggctaaa gcatcagaaa tatagtcgcc gtttaggttc agtgtagcaa tcacatcata
     9421 ttcggttggg tgtaacaaga tttcttgtaa aaaggcatcg gcaatgctgt cttttataat
     9481 gatttcctca ccggttttcg gattagtcaa gctcatccaa gggcctttat caattaattt
     9541 tgccccaaat tcttccgcaa cttggtagcc ccactcttta aatgctcctt cggtaaattt
     9601 cataatgtta cctttatgca caagcgtgag cgatttacgg ttgttatcaa ttacatattg
     9661 caatgcagca cgcaccaagc gttgtgtgcc ttgcttagaa accggcttaa ttccgatacc
     9721 gcaatcttcg gtaaagcgga tttttttcac gcccatctct tgttgtaaaa attcgatcac
     9781 tttattggct tcaggcgaac cggcaaccca ttctacaccg gcataaatat cttccgagtt
     9841 ttcacggaaa atcaccatat ccaccagctc agggtgttta accgggcttg gcgtaccgtc
     9901 ataataacga attgggcgta agcagttgta taaatctaat ccttgacgca tcgccacatt
     9961 taaagaacga ataccgccac ccaccggtgt cattaaagga cctttaatcg ccacgtgata
    10021 ttggcggata agctctaagg tttcatccgg caaccaagtg ttctcaccat ataatttatt
    10081 cgccttttcg cctgcgtaaa tttccatcca agcaattttt cgcttgcctt gataggcttt
    10141 ttctaccgcc gcatctagca ctgctttcat tgcaggagta acatcaacac cgatgccgtc
    10201 accttcaata aatggaataa tcggattatc cggaacttgt aaagaaccgt cttggttcag
    10261 ttgaatagtt tcgccttgtg gaatttggac ttgtgtttgc attgatttta cccccgtaat
    10321 gacgaaaaag tagtgacgaa aaatgaatgt tgacaatcag ccaacaatgg attaactata
    10381 aacagtaaat ttctaaatgt aaataaagca aaatgggctg agagagaatt tgtgatctaa
    10441 cgcacattta tgtaaataaa atgtaaaaaa tcgttgtgat tcttttggaa atcatctagt
    10501 ttatcttata aatctcaaat aaataggagt aagctatgtc aaaagcaaca ttaacactcg
    10561 aaacaggtga aaaacttgaa ctcaatatgc aaaaaggaac cttagggtat caaacgattg
    10621 atattcagcc tttcttaaag cataagttat ttgcttatga tcccggattg gtttcgaccg
    10681 ctgtatgccg atccaatatc acttatgttg atggcgatga aggggtttta ctctatcgag
    10741 gctacccgat tgaccaatta gccaaacatt ctgattactt agaagtcggt tatatgctgt
    10801 tattcggcga acgccctacc aaagaagaat acaaagactt cgttcaactc atcaaaaaac
    10861 acactcttgt acacgaacaa ctaaccaaat tcttctccgg tttccgccga gattcgcacc
    10921 caatggcggt gatgtgtggg gtaagtggag ctttggctgc cttctaccac gacgccattg
    10981 atgtaaacaa ccaagcccac cgtgaattaa ccgccattcg tttgttagcc aaaatcccaa
    11041 cccttgcggc gatgtgttat aaatactcta tcggtcagcc gtttatgttc ccacaaaacc
    11101 acttatctta cgccggcaac ttcttatata tgatgtttgc cactccttgt gaaccgtacg
    11161 aagtgaaccc tgtgttagag cgtgcgttag accgaatttt cattttacat gcagaccacg
    11221 aacaaaacgc ttcgacctca accgtgcgaa ccgccgcttc atcaggtgca aacccatttg
    11281 cttgcattgc tgccggtatt gcctctctct ggggacctgc acacggagga gcaaatgaag
    11341 cctgtatcaa tatgttgaaa gaaatcggca ctgtggatcg cattcctgaa tttattgctc
    11401 gagccaaaga taaaaatgac ccgttccgct taatgggctt tgggcatcgt gtctataaaa
    11461 actacgaccc tcgtgcgaaa gtaatgcgtg aaacctgcca cgaagtgtta aaagaactga
    11521 acattgataa cccactattt gaagtggcat tggaattaga acgtattgcg ttaagcgacc
    11581 cttatttcat tgagcataaa ctttatccaa acgtagattt ctactccgga atcgtgctaa
    11641 aagccatcgg tattccaacc tcaatgttta ccgtaatgtt tgccctagcc agaaccgttg
    11701 gctggattgc tcactggaaa gagatgtatc tccaaggcaa tatcaaaatc gttcgtccac
    11761 gtcaaatcta caccggacat actaaacgtg attttgagcc aatggataaa tcataagatt
    11821 ggcaaaaaat cgaaaaattt taaccgcttg tttccacaag cggttatatt ttgcataaaa
    11881 tttgcaaatc aggctaattt atttagcagt tgtgttctaa catcttgacc attttcatcg
    11941 ctttttacag ccataataaa taagcctttt ccttgactta atttctccca aagattgcca
    12001 attaaggctt tttctttaga atcttcatta ctttttaatt gttccccttt atattccaca
    12061 attaaaattc gaccatcaat aagctctaca ataaaatcag ggtaaaaatt ctgatgagct
    12121 aatggtaaag aaaaccctct ttttacagga tttcttaacc aatcatcaga aaaactcagt
    12181 tgctgctgat taaaatggta agaaacccgt ttatcttcca catcaatttc aaaactttgg
    12241 ctattttctt gtaatctaaa tccatcaagt ttggctggat aatccaataa attccaactt
    12301 tggtaatcta atagcacttg tgaatcagct aagtcgaatt cgccttgaat attcatacac
    12361 agttgaggta ttactttaaa tatttcccct ttttcggcag gagaagataa tatttcaact
    12421 ctattctggt gaatttgtaa ctgttgctca atttgctctt ttttcttacc tgttgcagtt
    12481 cgaatcaacg ctttttctaa cttttcactt aaattacctt ttacttgcac taaaatgtga
    12541 ccttcttgct cggtaatttt aagttgagaa cgttttgctt cgcttaaatc agacatttca
    12601 gggagttgcg ttaatgctaa aactgttgtc ggtgtataga ataaatctcc ttcgtttcca
    12661 atccaatttt cttgttgtgg gatacgaagc atttcagcta cttctaatgc ttcaaacccc
    12721 attccaatca atttatcttg taaattacga gccgtttcgc cgaacgactt agaggaaagc
    12781 ggagcataag cacgatttaa atcttcaatt tgacggtgtt tagcataagg cattcgcaat
    12841 actcgcccta atagctgctc cgcttccttg ctagaggaaa cattttgtac tgagcaaaaa
    12901 atataagcaa aagggcaatc ccacccttct tttaatgcct caaccgtgat gatgtaattg
    12961 atcttgcatt gtggattaaa taaatcgata ttatctaatt cgtgttgatt acctatcaca
    13021 atggcaatct cttcttcatc aatttttaac tcatcaatta aatactgttt taagacttct
    13081 actgttactt tgccgttttt cggctcagct tgaaacaata aatcggacga acgtactcgc
    13141 tctcatactg tgctttttgt gctaatgctc ctctattgat tacagcccca tctattgctt
    13201 gttgccagtt gtgatgttcg gtcagcacaa taggcaattt gatcatctct tctgctttta
    13261 gatggcttgc cgaaacgtga tacaacacat tacttcctgc ttttctatct gtgtttggcg
    13321 tagcagtaaa ttctaaaata gctgatgggg aaagttgaga tagcacatca aaagagagtg
    13381 aagtccgagc attatgtgct tcatcaacaa tgaccaaagg ctgataagct cgcaacaaat
    13441 ttgcaaaaga acacttaatt ttgcccgttt gtttggaggt taaaccgttc tcttgtagat
    13501 cagtagtgga aatcttttct aaatttgcct gaatatgggg tagtaaacgc tcaaaatgag
    13561 ctgttaattt ttcattaaaa gcatagattt ttcgcccatc ttgattttct acccgaaaat
    13621 tttgaatagt agaaacgatt acaatcgcct tattaccaaa atcttgtggg cgaagtaaat
    13681 caaaatcttc agactcaata accattactt ctcggttaaa agttttatca agtactgcac
    13741 ggtaagggtg ttgcggattt tttaatgctt ctgtagtttg ggttttaatt gtacgagaag
    13801 gcactggcca caaggtaacc gggaaatcgc aatccaaata ctgctttgca atacgagcaa
    13861 tactatgcga ggctaacaac gttttaccgc caccagttgg aattcgtaca caaacataag
    13921 gcacatcacg caaattactg tgttcacttg ggatcttgta actatattca ggttgaattt
    13981 ggctaaatgc ggtttttgca tcagattgca aacaagccga aaaatagtgg ctgacacggt
    14041 ttaatgcctc attttggtaa ttttttaatt tcattttttt ctcctactat taacgagaca
    14101 ttacatcata tggaatctgt ttaaatgtaa ttttcttttc agctaactgc acttctccta
    14161 aacggcacgc ttcgccatat accacaatat ccattccctg ttgaataaat tcagcaaagt
    14221 atggtaactc cgccattaat tttcgtgtca gaacattacc actctgcgga cgtttatcgc
    14281 ctaaaattcc gttatataac aaatagtagg ctttgccgtt gaaagtgccg acaagcggtg
    14341 atttttcgct gtttttttgc aaagggaaat gattttctaa ataccaaata tgggcggcta
    14401 aatcgtcaaa acgaatgttc ggattaagtg aaccgaatgc gtcaaatact tcctcgccta
    14461 actcacaaaa gcgaaacgat ccaccacctt gccaatttac tgcttttgaa ataccgcctt
    14521 gctcaccttc aattactttt ttcaaccgtg ggacaacatg ggttttagca tgttcgccaa
    14581 tctcaatacc gatataacga cggttcattt tatgagcaac tgctgcagtt gtacctgagc
    14641 caagaaagct atcgaggacg agatcatatg gttcagtcga tgcttctaat acaaattaga
    14701 ttaatttttc aggtttagga taatcaaatg aatcttctgc aatatcctcc ccaaataatt
    14761 ctagatgttc actagcagag tcttcatttg ttgccatatc ataatgagct ctgctaatta
    14821 agttatgaat ttttttcttt tccgcacttc gctcaaatac tctcct
//
LOCUS       DANLXS010000047        14734 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.047, whole genome shotgun sequence.
ACCESSION   DANLXS010000047 DANLXS010000000
VERSION     DANLXS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14734
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.047"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..579)
                     /locus_tag="PXD71_001042"
     CDS             complement(<1..579)
                     /locus_tag="PXD71_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HDL6291177.1"
                     /translation="MMSFLSEVFSSFKPTKAGVISSEPNENTGESDIIQNKMSEVNNE
                     VQQLKRSGKLTQSQHHRRLYSQEPSFIDHLPWGEYLNEHGVILLDDARSVGAVFDIKP
                     IGTAGRSIEFLSRIRNLVKDALQDSFDELDINPYVVQFYCQDSDDLQPYIEQLRNYIS
                     PIAQNSAFSEEWLRVQAKHFKDIGKPEGLFIDD"
     gene            complement(579..977)
                     /locus_tag="PXD71_001043"
     CDS             complement(579..977)
                     /locus_tag="PXD71_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL6291178.1"
                     /translation="MKHLFCIMISALLITGCTTSTEELLPTNGSTMRDIWDKGASNND
                     LHLYRSQQGRTLDPVDYIPNSEQRAYTRTAENEINNLFPRLPNPDLIMYVYPHQTRSG
                     EPLSIPGYSTVLPFYGRVQYAQPGDRTRGL"
     gene            complement(990..2444)
                     /locus_tag="PXD71_001044"
     CDS             complement(990..2444)
                     /locus_tag="PXD71_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL6291179.1"
                     /translation="MKANKALFVILGIVFILVGGIVYALFGRSESVPQPTQQEILDIS
                     IKDLTPEQVRTMGIQGDTPIDTLRTLVTTVKSNERRMDELIDENRQLKQKEDLVNGRI
                     NDAVNNETRKLQEQIADLKMQLFDMATLVNNKPTTQESTPQVEAQPNTVPNKGFGTAG
                     DKLPIGGATMSNVDTSGMYWVEPSDQISLDASGKPVSGNTVATKFGFPKDFNGGQSAG
                     NNEKKGLPFFPETDTVTSSQGTAGNARGKNQMTSVYTIPSNSTFLDSVAMTGLIGRIP
                     IDNRVSDAYPFKVLIGRENLIANGIELPDVDGAIVSGTATGDWTLSCVRGSVKSMTFV
                     FNDGRIVSTSNKGNGEEIGWLSDSSGIPCVPGERKTNAPEYLGTNFFLAAASAAAQGF
                     SQSQTTTVVDGSSVVGAVTGDQGKFILGQAIGGGLKESADWFRKRFAENFDAVYVPPG
                     HRVSIHIEKEIEIDYNAQGRKVKYGSKSTNSQLD"
     gene            complement(2456..3340)
                     /locus_tag="PXD71_001045"
     CDS             complement(2456..3340)
                     /locus_tag="PXD71_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HDL6291180.1"
                     /translation="MKRLPILLVSLLIGFSGSSLADVLMKWERKPLPVALQVEKERII
                     FVDKNVKVGYPAELDGKLRIQSTGGAVYLKALETFPSTRIEFRDVTTGEIFLFDLTAA
                     SKVANPEEAIRVVFDGTVEKQSFSSNRDDESSDLPIANSRPQLPVPAALTRYAAQSLY
                     APLRTVEPLDGVRRVAHRLPKKITKLLPAYSIEATPLISWQLDEYVVTAIRLQNKGNT
                     QITLDPRELQGQFYAATFQHNWLGGYGENEDTTTLYLVTEGYANNALIPETRPVKLVS
                     KKVKRTTKVETKPAQSAK"
     gene            complement(3340..3984)
                     /locus_tag="PXD71_001046"
     CDS             complement(3340..3984)
                     /locus_tag="PXD71_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HDL6291181.1"
                     /translation="MSQLKSQLHEKDKHIKTTRIFAFLFFIIILVLAGVIYTFPSRLT
                     IYNPPDLRAGSQRAWWEIPKPTIYAFSYQIFQQMNRWMTNGEEDYEKNITTLKPFLTP
                     HCEAFLRQDYKDRLRNNELRSRTRMVYEITGRGYSEDKVTIFSEDSWAVNLDLSVDEH
                     FQGQPVKRVYIRFPISIVRMDVDSAQNPWGLGFNCYHSIPKLLEGVEPKTEEKK"
     gene            complement(3988..4353)
                     /locus_tag="PXD71_001047"
     CDS             complement(3988..4353)
                     /locus_tag="PXD71_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HDL6291182.1"
                     /translation="MSEQDTTIPFLPTRLNREATVYGGMTVSEFGISAGLGFMLGLIV
                     GLFLWILTDFWLLIPAMAMLLCIATVLIGKGIVAAVKRGKPEAYLNRLVEKKMDDLFN
                     GHKFIKREGFWSIRRYRRK"
     gene            complement(4376..4765)
                     /locus_tag="PXD71_001048"
     CDS             complement(4376..4765)
                     /locus_tag="PXD71_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2976 domain-containing protein"
                     /protein_id="HDL6291183.1"
                     /translation="MNRIKSLTKHLHSRYLAVMLFFWGSLQNAFAAGPDASKIPDFQI
                     PGVDSNTKDPMEIIWIVLKGLVTLGAVILAAVAILTVVKALIGIYNEATSENGKKGWG
                     HFILALIIGFIVIFFCLWLVKLAVGLF"
     gene            complement(4780..5013)
                     /locus_tag="PXD71_001049"
     CDS             complement(4780..5013)
                     /locus_tag="PXD71_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HDL6291184.1"
                     /translation="MSYSPANAFSVASGIDPFRLKIMIAIIFLSLLLLAVAWALNSGY
                     KGFSFRDKSPFSLLVLFLKGVALLACVTYFFIY"
     gene            complement(5013..5339)
                     /locus_tag="PXD71_001050"
     CDS             complement(5013..5339)
                     /locus_tag="PXD71_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HDL6291185.1"
                     /translation="MSISTSLRKGIVVLVTLSCLPALASTEQEQLAQAIKQLEAAKLS
                     LQRAEKVAKASPKTREYFNYSAIHSDIETVKKGIQQYINPSRALPRDPNVLRTLSDDY
                     SKLRGQ"
     gene            5526..5909
                     /locus_tag="PXD71_001051"
     CDS             5526..5909
                     /locus_tag="PXD71_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291186.1"
                     /translation="MRYQMRYLALFLVVFPYVAHSLPSAQKVGNICHAENAQYNYGGL
                     WELSLRGKLICNYWQFLGDDERKKIELQLDEYGEFPIWNTSEQISEQIIEDDEYPQYW
                     EVEDWEDSDWDTESENEDTEATTEE"
     gene            complement(5941..6636)
                     /locus_tag="PXD71_001052"
     CDS             complement(5941..6636)
                     /locus_tag="PXD71_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HDL6291187.1"
                     /translation="MSEETKARRKSGLINLIFGKINMSLAAIAVSLIFSIILEWIGIA
                     FWWPQEGHLHSQTMMLNEMQWLSDDFSQSLFAKSSTAFATSIIENVYNWLFVYTGVKE
                     WLTSPGNGSMEQWVHHYGRAYIESVIYVVITFIIRLIIIVFTSPLFLLTALSGLTEGL
                     SLRDIRKFGAGRESAYLYHHARRYIMPMMVTAWVLYLSIPFSIHPNIILVPAACLFGL
                     SICITAASFKKFL"
     gene            complement(6633..6986)
                     /locus_tag="PXD71_001053"
     CDS             complement(6633..6986)
                     /locus_tag="PXD71_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6291188.1"
                     /translation="MSSAKMREEKRTNLLDLPNKYRNFNGEFSASCGLDNAEELLIHS
                     QSYFIEWFEQGYSFHQFAEKFADQGLSLWSADEVSMRHSDKSKDIFAFYLAFDNNPSG
                     YILVQCQLDREDSLQ"
     gene            complement(6979..9180)
                     /gene="traD"
                     /locus_tag="PXD71_001054"
     CDS             complement(6979..9180)
                     /gene="traD"
                     /locus_tag="PXD71_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDL6291189.1"
                     /translation="MSAKHTLEAWLRPPVEFFPAAVHASCAFVSAAAPWSLALHPSIG
                     YSIAAGFGALSFYRAKQGWQIVRYHRNLKRLPNYSMTSKQIPVSNKYLFVGKGFEWKP
                     IHTQRLHDCFSDNGSKYLEPSKLFKMAREYEKRHENWLTHLTSKDSRWNLVRPLPPVE
                     GIPAIHGIEPDEVDVVQSVSSRVGHTVVFGTTRVGKTRLAEVFVTQDIHRGKTADERE
                     VVVFFDPKGDADMLKRMYAEAKRAGREDEFYVFHLGYPEISARYNPVGRFNRISEVAG
                     RISGQLSGAGNSAAFKEFAWRFVNIVARALVELGNRPNYVQISQFVQNIDSLFLDYAE
                     KFIRKTDPKIWENLATIAAGLDEKALPFGMKDRPLIFVINKYIAESAVFDPILQGLAS
                     AVRYDRTYFDKIVASLLPLLEKLTTGKISELLSPDYADILDERPIFDWESIIRKRGIV
                     YIGLDALSDATVAAAVGNSMFADLVSMAGHLYKFGTNDGLPGAFKEKSKPVKINLHCD
                     EFNELMGDEFIPLINKGGGAGIQVTAYTQTLSDIEARIGSKPKAGQTLGNFNTLYMLR
                     VKEPATAELLTKQLHEVTIKRSTVTSSTTDSSNPDDDKAFSSNTGQRISESTVPMLVP
                     ANITNLPKGQAFALMDGSKLWKIRMPLPAVDKDDMMHQSLRELTDYMRKNYHVVENWW
                     QPAFTEYTPSRDIAEAFEAMSEPQYKTQIDHQVDNFGIDEPYDIDEEENDE"
     gene            complement(9180..9686)
                     /locus_tag="PXD71_001055"
     CDS             complement(9180..9686)
                     /locus_tag="PXD71_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL6291190.1"
                     /translation="MQNKVKVLLFSLYVSHFSWADLTVIADFGGESAVRFYESLQPEH
                     DENAPNYPNSISASVSEADILPIVSHKLSPGTIKEKPFDLTGMQAIFLIGADNTSAQW
                     LQQNREKLITLNATGLVVNVQTLSELNQLRDIVPELRILPTPGDDLAERIGIAHYPVL
                     ITESGVSQ"
     gene            complement(9689..10459)
                     /locus_tag="PXD71_001056"
     CDS             complement(9689..10459)
                     /locus_tag="PXD71_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HDL6291191.1"
                     /translation="MDITRVLVIVAVFLSNISISQAKPFNGFGSEIDSYIRMAAQRYQ
                     VSEAMLRGFVKMEDGWYGNISPTGATGVGQFTMGTWNWLSETERGKQLGMKLITAKNR
                     GSYADPRRDKYTNTLATALLARWHIEQFQLRNIPITDENLYMAHNIGLDGLHRAMMGK
                     ATKEDILNMRRNGMKSWMTVRDFLNYQKGRFLAHKQEANFMTVSKQGHIHTGVAAFVY
                     PTSQMGKSVKWVEPDNKMIWVEPKNAMVWVEPQNTLQR"
     gene            complement(10438..11211)
                     /locus_tag="PXD71_001057"
     CDS             complement(10438..11211)
                     /locus_tag="PXD71_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HDL6291192.1"
                     /translation="MREISMKFNLCRKAIPLAVLPLLLSFALPTLAQNMQTETVSSSQ
                     STLQKQSQHQQSQLSDSLKQAKEWGLTVEEWSRYTELMKGERGMWSPNLDPLTALGIE
                     ARTAEEREKYARMLAKKYYERVSKELDFDKVYRQEFEKLYPNELPFEVEPHISQSVGR
                     VVYFTRFDNCDSCKSDLGKILSYANNQTPLDIYIVGQNISDNDIRKWAADNNIDPIKV
                     SKRLITLNHDKGYWLRYSLGKMPAAYQIKQDGEWTSLVY"
     gene            complement(11211..11840)
                     /locus_tag="PXD71_001058"
     CDS             complement(11211..11840)
                     /locus_tag="PXD71_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291193.1"
                     /translation="MKTKISLMTVFMLGLSGCVHQQDTAQNSDEITPMPDSLLTPQMQ
                     QLPPQDRIVSKDIYQEQTTSNTEVVRDGRYTLISIAPEEGQKYLLEQVVSVKVSNPKN
                     RFTANVQQGLRSTLTNTGLDLCYPPIGEQGNAVQTLFTRPLPKVHYQFGPMKLYEALQ
                     MIAGPAYELTLNDISRTICFKARESSFQNPHKPAVAVTTTTIKTEVIEE"
     gene            complement(11999..12259)
                     /locus_tag="PXD71_001059"
     CDS             complement(11999..12259)
                     /locus_tag="PXD71_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291194.1"
                     /translation="MTFELGRVCTTRAVAECFNQNALVELLNRYCYGDWGNLCEEDKL
                     ANQEAMKYGGRIVARYDVDDESLYIITEADRSCTTILFSYEY"
     gene            complement(12372..12734)
                     /locus_tag="PXD71_001060"
     CDS             complement(12372..12734)
                     /locus_tag="PXD71_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291195.1"
                     /translation="MEDSFFDFDDISCYLGQWEAILEEYSDIVSIEDFWLVAKEFETV
                     PHFGNLYQELVISRLIQRFCTELDIEQDSDLVEFDYYINAIDTHFYINRQRICDIDDW
                     NEMLDKIRKEMTPAKLAA"
     gene            complement(12814..13221)
                     /locus_tag="PXD71_001061"
     CDS             complement(12814..13221)
                     /locus_tag="PXD71_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL6291196.1"
                     /translation="MNRQGIYINGKQVFDSKGKEINSVQIINSTVQTVVGINKGYISM
                     AAGNNVLIVDGQTIALEPSPTINIEVFGNVTNLNAGSENVRVTGDAKSVSTVSGDVTV
                     DGNVTSNVKTVSADIHCKSILGKINTVSGDIYR"
     gene            complement(13247..13927)
                     /locus_tag="PXD71_001062"
     CDS             complement(13247..13927)
                     /locus_tag="PXD71_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291197.1"
                     /translation="MNSLQQFVKTLSPTVLNGRHFDGIVGDVVFVQNGYGKWLDQGFT
                     IIGFDKSIEEKDWIYLDWDCYWFGKNPAQVIPEKSWAQRSWLARAYSISLERFNSLGL
                     EREYNIVSDWDNPIYGTDIQLIEHSKEFFRWTEADDRGIFLVHNEMAFHFFKKEIKAS
                     SEPEYYQPIETFWKDATVVLNIEDLDMLERVGLEQTNTPALAKLTTFVQAARNEISNG
                     NIVFYRAF"
     gene            complement(14033..14464)
                     /locus_tag="PXD71_001063"
     CDS             complement(14033..14464)
                     /locus_tag="PXD71_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL6291198.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEHKKGNPFYACRVAAL
                     VGESDSPEYRYFDMNVVGKETETLIKKCQKAVSEEKKVLVSFVMADLWFDTFTYSSDS
                     KYHKKGDTGVSLKGRLIRIKMIKIDGEIVYQEKPKADEQSE"
BASE COUNT         4365 a         3053 c         2809 g         4507 t
ORIGIN      
        1 gtcatcaata aaaagccctt ctggtttacc aatatccttg aaatgcttgg cttgtactcg
       61 taaccattct tcgctaaatg cactattttg ggcaataggc gagatataat tccgtagctg
      121 ttcaatataa ggctgtaaat catcgctatc ttggcaataa aattgcacga cgtaaggatt
      181 tatatccagc tcatcaaagc tatcttgtaa ggcatctttg acaagattac gtatccgact
      241 taaaaattca atactacggc cagcagtacc aatcggctta atatcaaata ctgccccaac
      301 actacgggca tcatcaagga gtatcacacc gtgctcattc aaatattctc cccaaggtag
      361 atgatcgata aaggagggct cttgtgagta taaccgacgg tgatgctgtg actgggtgag
      421 ttttccactg cgttttaatt gttgaacctc gttatttacc tcactcattt tattttgaat
      481 gatgtcagac tcaccggtat tttcatttgg ttcacttgat ataacccctg ctttagtcgg
      541 tttgaaagag gaaaatacct cacttaaaaa agacatgatt aaagccctct tgttctatca
      601 cccggctgag catattgaac tcgtccatag aaaggtagta ctgtagaata tcctggaata
      661 ctgagaggct ctccggaacg agtttgatgt gggtacacat acataatcag atccggatta
      721 ggtaaacgag ggaacaagtt attaatttca ttttctgccg tacgggtata ggctcgttgc
      781 tcactattag gaatataatc gacagggtca agtgttcgcc cttgttgtga acggtataag
      841 tgtagatcgt tattactcgc tcctttatcc caaatatccc gcatcgtact gccgttagta
      901 ggtaataatt cttctgttga tgttgtgcaa cctgttatca ataaggcact tatcattatg
      961 caaaataaat gtttcatcgt ttgttccttc taatccagtt gtgaattcgt tgattttgag
     1021 ccatatttca ctttacgacc ttgggcgttg taatcgattt caatctcttt ctcgatatga
     1081 atagagacac gatgacccgg tggcacatat acagcatcaa agttttcagc aaaacgtttt
     1141 ctaaaccagt cagcagattc ttttaagccg cctccgatag cttgtcctaa gatgaactta
     1201 ccctgatcgc cggtaacagc tccaacaaca ctacttccat caacaacagt ggttgtttgt
     1261 gactgtgaga atccttgggc agcagcactt gcagcagcca aaaagaaatt cgttcccaaa
     1321 tattctgggg cattggtttt ccgctctcct ggaacacaag ggatacccga actatctgat
     1381 aaccagccaa tctcttcgcc attcccttta ttagaggtac tcacaatacg tccatcattg
     1441 aacacaaaag tcatactttt tacactgcct ctaacacagg atagtgtcca gtcgccagta
     1501 gcagtacctg atacaattgc tccgtctaca tcaggtaatt caataccatt agcaatgaga
     1561 ttttctcgac caataagtac tttgaacgga taagcatccg atactcgatt atcaatcggg
     1621 atacgtccga tgagtcccgt cattgcaact gaatcaagga atgttgaatt acttggtatg
     1681 gtatatactg atgtcatctg gtttttacca cgtgcgtttc cagccgttcc ttgtgagctt
     1741 gtcacagtat ctgtttccgg gaaaaacggc agacctttct tttcgttatt tcctgcagat
     1801 tgtcctccat taaaatcttt cggaaagcca aatttagttg ctactgtatt gcctgacaca
     1861 ggttttccac ttgcatccaa actaatctga tcgcttggtt ctacccaata cattccactg
     1921 gtatccacgt tggacatagt tgcaccaccg ataggtaatt tatctcctgc tgttccaaat
     1981 cctttattcg gtaccgtatt aggctgagcc tctacctgag gggtagactc ttgtgttgtt
     2041 ggtttgttat taacgagagt cgccatatca aataattgca ttttcaaatc tgcaatctgc
     2101 tcttgcagtt ttcgggtttc attatttacc gcatcattga tacgaccatt cactaaatct
     2161 tctttctgtt tgagttggcg attttcatca attaactcat ccatacgacg ctcattggat
     2221 ttaacggttg tcacaagggt acgcagcgta tcaatcggcg tatcaccttg aatacccatt
     2281 gttctgactt gttcaggtgt taaatcttta atggaaatat ctaaaatttc ctgttgtgtc
     2341 ggttgtggaa ctgactcact acgtccaaat aaggcataga caattccacc gactaagata
     2401 aatacaatgc ccagtatgac aaataaggct ttattcgctt tcattgcctt tcctcctact
     2461 tcgccgattg agccggttta gtttcaactt ttgttgttct tttcactttt ttactgacta
     2521 gtttgactgg acgggtttcc ggaattaatg cgttattagc atagccttcg gtcactaaat
     2581 acagggttgt ggtatcttca ttttctccgt accccccaag ccaattatgc tggaaagtcg
     2641 ctgcataaaa ttgtccttga agctctcgag ggtcaagcgt aatttgagtg ttacccttat
     2701 tttgcaaacg aatagcggta actacatatt cgtctaactg ccagctaatt aaaggtgttg
     2761 cttctattga ataagccggt agcagcttgg taattttttt aggtagacga tgtgccacac
     2821 gacgcacacc atctaaaggt tcaactgtac gtagtggagc ataaagtgat tgtgctgcat
     2881 aacgggtcaa tgcggcaggt actggaagct gaggacggct attcgcaata ggcagatcag
     2941 agctttcatc atctctattg cttgaaaaag attgtttttc taccgtaccg tcaaaaacca
     3001 ctcgaattgc ctcttcagga ttagcgactt tcgaagctgc tgttaaatca aatagaaaaa
     3061 tttcaccggt tgtaacatca cggaattcta tgcgagtgga gggaaatgtt tctaatgctt
     3121 ttaaatagac ggctccaccg gtagattgaa ttcgtagctt accgtccagt tctgccggat
     3181 aaccaacctt gacattttta tcgacaaaaa taattcgttc tttttccact tgtaacgcaa
     3241 caggaagtgg ttttctttcc catttcatca atacatcagc caagctactc ccagagaagc
     3301 ctataagcaa agagacgagc aaaattggta gacgtttcat tattttttct cctctgtttt
     3361 tggctcaaca ccttctaaga gttttggaat ggagtgatag caattaaacc ctaatcccca
     3421 agggttttga gcagaatcga catccattct cacaatactg ataggaaaac gaatataaac
     3481 tcgttttacc ggctgtcctt ggaaatgttc atccacactt aaatcaagat tgacagccca
     3541 actatcttca ctgaatatgg taactttatc ttcactatat cctctaccgg taatttcata
     3601 caccatacgg gttcggctgc gtagttcatt gttacgcaga cgatctttat aatcttgtcg
     3661 taaaaatgct tcacagtgtg gtgttaaaaa tggtttgagg gtggtgatat ttttctcata
     3721 atcctcttcg ccatttgtca tccaccgatt catttgctgg aaaatttgat aggagaatgc
     3781 ataaattgtt ggtttaggaa tttcccacca agctcgttgg ctcccagcac gcagatctgg
     3841 tgggttgtat atcgtaaggc gacttgggaa agtatagatt acgcctgcca gtaccaaaat
     3901 aataatgaaa aacaggaagg caaaaattcg agtcgttttg atatgtttat ctttttcgtg
     3961 taattggctt tttaactggc tcatttgtta tttccttcta tatcggcgaa tagaccaaaa
     4021 cccttctcgt tttataaact tatgaccgtt aaagaggtca tccattttct tttcaactaa
     4081 tctatttaaa taagcctctg gttttcctcg tttaaccgca gcgacaatac cttttccgat
     4141 taagacagtt gcgatgcaga gcaacattgc cattgccgga ataagaagcc aaaaatcagt
     4201 taatatccac aagaagagac caacaatcag acctaacata aatcctaatc cggcagatat
     4261 gccgaactca ctaactgtca tcccaccgta aacggtagct tcacgattaa ggcgggtggg
     4321 aagaaaagga atggttgtat cttgttctga catattggtc tcctagacat tttaattaga
     4381 agagtccgac tgcaagtttg actaaccaca agcagaagaa aatcacaata aagccgataa
     4441 ttaaggcgag aataaagtgt ccccaacctt ttttaccatt ttcagatgta gcttcgttat
     4501 agatgccaat caatgcttta acaactgtca aaatggctac ggctgctaaa ataacagctc
     4561 cgagagtgac aagacctttc agaacaatcc aaataatttc cattggatct ttggtattac
     4621 tatccacgcc tggaatttgg aagtctggga ttttggaagc atcaggaccg gctgcaaatg
     4681 cattttggag ggaaccccag aagaagagca ttacagccaa ataacgacta tgcagatgtt
     4741 tcgtaagaga tttaatgcgg ttcataatag acctcgaatc taatagataa agaaataagt
     4801 aacacaagct aacagtgcca ctcctttgag aaataaaact aaaagtgaaa aaggactttt
     4861 atctctaaat gaaaatccct tataaccact attcaatgcc caagcgacgg ctaagagcag
     4921 taatgaaaga aaaataatgg cgatcatgat tttcaatcta aaagggtcga taccgctggc
     4981 aacagaaaac gcattcgcag gggagtaaga catcactgcc cccgtaattt gctataatca
     5041 tcacttaatg tacgtagtac attcggatca cgaggtaatg ctcggcttgg attgatatat
     5101 tgttgaattc cttttttgac ggtttcaata tcactgtgaa tagcagaata gttaaagtat
     5161 tctcgtgttt taggcgaggc tttagctact ttttctgctc gttgaagtga taattttgcc
     5221 gcttccagtt gcttaattgc ctgggctaat tgctcttgtt ctgttgaagc aagtgctggc
     5281 aagcaagata aggtaacaag gacaacaata cctttcctta aagatgttga aatagacata
     5341 gtgcacctct atttttagtt ttacttcagg ttaagagtgc caaaagcgat cctaaaattt
     5401 aatggaaaac tctgttaccg ttcagaagag aaattgcaca aaatagtaaa tggtgagaaa
     5461 gatattattc tatgtttaag ctagtcaatt aagtagtatc aacaccgaaa tcttcacgct
     5521 ttacgatgag ataccagatg agatatctag cactgttcct tgtagttttc ccatatgttg
     5581 cccattctct tccttcagca cagaaagtgg ggaatatttg ccatgcagaa aatgctcaat
     5641 ataattacgg aggtctttgg gaattaagtt taagaggaaa gttaatctgt aattattggc
     5701 aatttctagg cgatgatgag cgaaaaaaga tagagttaca attggatgag tacggggaat
     5761 ttcctatttg gaatacctct gaacaaattt ctgaacaaat cattgaagat gatgaatatc
     5821 cacaatattg ggaagtcgaa gactgggagg attctgattg ggatactgag tcagaaaatg
     5881 aagatacaga ggcaacaaca gaagaataag cccccgcata ttgctaaggg cttatgaaat
     5941 ttacaagaat ttcttgaaac tcgccgcagt tatacagatg gataaaccaa acaaacaggc
     6001 tgctgggact aaaatgatgt tcggatgaat tgaaaacggg atagataaat aaagcaccca
     6061 agctgtgacc atcattggca taatataacg tctagcgtga tggtataggt aggctgattc
     6121 ccgcccggca ccaaattttc gtatatctcg taatgacagt ccttcggtta aaccggaaag
     6181 tgcagtcaat aaaaagagtg ggctggtaaa aacaataata atgaggcgaa taataaaggt
     6241 gatcaccaca taaataacag attcaatata ggctctgccg tagtgatgta cccactgttc
     6301 catactacca ttacccggtg aagttaacca ctccttaaca cccgtataaa cgaataacca
     6361 gttatacacg ttttcaataa ttgatgttgc aaaagcagta gaagacttgg caaacaggct
     6421 ttgagaaaag tcatccgata gccattgcat ctcattgagc atcatcgttt ggctatgcaa
     6481 atgcccttct tgcggccacc agaaagcaat gcctatccac tctaaaataa tgctgaatat
     6541 gagagatact gcaattgcag caagcgacat atttattttt ccaaaaatca ggttgattaa
     6601 acctgatttt cggcgagctt tagtttcttc actcattgca aactatcctc tctatcgagc
     6661 tggcactgga caaggatata gccggaagga ttattatcaa aagcaagata aaaagcgaaa
     6721 atatctttgg atttatccga gtgacgcata gatacttcat cggcagacca taacgaaagc
     6781 ccttgatctg cgaatttttc ggcaaactga tggaaggaat aaccttgttc aaaccattca
     6841 ataaaatagg attgactatg aatcaggagt tcttctgcat tatctaagcc acaggaagct
     6901 gaaaactccc cattaaaatt acggtattta tttggtaaat ctaatagatt ggttcttttt
     6961 tcctctctca ttttggcact actcatcatt ttcctcttca tcaatatcgt aaggttcatc
     7021 tatgccgaaa ttatctactt gatggtctat ctgtgtttta tattgaggct ccgacatcgc
     7081 ttcaaatgct tctgcaatgt ctcgggacgg cgtatattct gtaaatgccg gttgccacca
     7141 attttcaaca acgtgatagt ttttacgcat ataatctgtc agctctcgta aagattgatg
     7201 catcatatca tctttatcga cagcaggtaa cggcattcga attttccata atttactacc
     7261 gtccattaaa gcgaatgctt gtccttttgg taaattggta atgttggccg gaaccaacat
     7321 aggaacggta ctttcgctga tacgctgtcc ggtattggag ctaaacgcct tatcatcgtc
     7381 aggattactg gagtcagttg tactggacgt gaccgtgcta cgtttaatcg tcacttcgtg
     7441 taactgtttt gtgagtaatt ctgccgtagc cggttctttt accctcagca tatagagtgt
     7501 attgaagtta cctaaggttt gtcccgcttt cggtttactg ccgatacgag cttcaatgtc
     7561 ggagagtgtt tgtgtataag cagttacttg tattccagca ccaccgcctt tgttgatgag
     7621 agggataaat tcatcaccca tcaattcatt aaattcatca caatgaagat taattttcac
     7681 tggtttggat ttttctttga atgctcccgg taatccgtca tttgtcccaa atttatataa
     7741 atgacctgcc attgagacta agtcagcaaa catactgttt cctacagcgg ccgcaactgt
     7801 cgcatcagag agagcatcta aaccaatata aacgattcct cgtttacgaa taatggattc
     7861 ccagtcaaaa atcggacgtt catccaaaat atcagcataa tccggggata acagctcaga
     7921 aattttccct gtcgttaatt tttccaataa cggcaacaat gaggcaacaa ttttatcgaa
     7981 ataggtacgg tcatagcgta cagcactggc aagtccttgt agaattgggt caaagactgc
     8041 actttcagca atgtacttgt tgatcacaaa aatcagcgga cggtctttca ttccgaaagg
     8101 aagtgctttt tcatccagtc cagcggcgat ggttgctaaa ttttcccaaa tttttggatc
     8161 ggttttacga atgaattttt ccgcataatc aaggaataat gaatcaatat tttgtacaaa
     8221 ctgggaaatt tgaacatagt ttgggcgatt tcccaattca actaatgcac gtgcgacgat
     8281 attgacaaat cgccaagcga actctttaaa tgccgccgag ttacccgctc cacttagctg
     8341 tcctgaaata cgcccagcga cttccgaaat acgattaaag cgtcctactg ggttataacg
     8401 ggcagaaatt tcgggataac ctagatggaa aacataaaac tcatcttctc gccccgctcg
     8461 ttttgcctcc gcatacattc gtttgagcat atcggcatcg cctttcggat caaagaagac
     8521 taccacttca cgctcatcag cagttttacc tcggtggata tcctgggtaa caaagacctc
     8581 agctaaacga gttttaccga cacgtgttgt accgaatact accgtatgcc caacccgaga
     8641 gctaacggat tgaacgacat caacctcatc cggctcaatt ccatggatag caggaatacc
     8701 ctcaacaggt ggcagaggtc taaccagatt ccaccggcta tcctttgagg taagatgtgt
     8761 tagccaattt tcgtgtcgtt tttcatactc acgagccatt ttaaatagct tagaaggctc
     8821 cagatatttt gaaccgttat ccgaaaaaca atcgtgtaac cgttgagtgt gaataggttt
     8881 ccattcaaat cccttaccga caaacagata cttattactg accggaattt gcttgcttgt
     8941 cattgaataa ttaggcaaac gtttcaggtt gcgatggtaa cgcacaattt gccaaccttg
     9001 ttttgcccga taaaagctca atgctccaaa accagctgca atactgtaac cgatacttgg
     9061 atggagtgcc aaagaccaag gagctgcagc actgacaaag gcacaagaag cgtgaaccgc
     9121 tgccggaaaa aattcaacag gaggtcgtaa ccacgcctct aaggtatgtt tagcagacat
     9181 tattgtgaaa ctccgctttc agtgattaag acagggtaat gtgcaatacc aattcgctct
     9241 gcaaggtcat ctccgggagt tggtaatatt ctcagttccg gtacaatgtc tcgtaattga
     9301 ttaagttcgg agagtgtttg tacattcact acaagccctg ttgcattcaa cgtgatcaac
     9361 ttctctcgat tttgttgtaa ccactgagct gaggtattat ctgctccaat taagaaaatg
     9421 gcttgcattc cggttaaatc aaatggtttt tctttaatag tcccgggaga tagtttatgg
     9481 gaaacaatgg gtaaaatatc agcctcactc actgaggctg aaatggaatt cggatagttt
     9541 ggtgcatttt catcgtgttc cggttgcaaa gattcataaa aacgtaccgc actttcacca
     9601 ccgaaatcag caatcacggt taaatcagcc caagagaaat gggacacata taaactaaac
     9661 aataatactt ttaccttgtt ttgcattttt acctctgaag agtattttgg ggttctaccc
     9721 aaaccattgc atttttaggt tcaacccaaa tcattttatt atccggctca acccatttga
     9781 ccgattttcc catttggctt gtgggataaa cgaaagctgc aactccggta tgaatatgcc
     9841 cttgcttgga gacagtcata aaattggctt cttgcttatg tgctaaaaaa cgtccttttt
     9901 ggtaattgag aaagtctctt accgtcatcc aacttttcat tccattacga cgcatattta
     9961 aaatgtcttc tttagtagct ttccccatca ttgctcgatg cagcccatct aagccgatat
    10021 tatgagccat atataaattc tcatcagtaa tagggatatt acggagttga aattgctcaa
    10081 tatgccaacg tgccaataat gccgttgcta aggtattcgt atatttatct cgacggggat
    10141 cggcgtaaga accccgattt ttggctgtaa tcaacttcat accaagttgc tttcccctct
    10201 ctgtttcaga tagccagttc caggttccca tagtaaactg cccaacaccg gttgctcctg
    10261 ttggtgaaat gttgccatac cagccatctt ccattttgac aaaacctcgt agcattgctt
    10321 cactcacctg ataacgttga gcagccatac gaatatagct gtcaatttca gaaccaaatc
    10381 cattgaaggg ctttgcctga gaaatgctaa tgttggaaag aaagactgca acaatgacta
    10441 atacacgagt gatgtccatt caccatcctg tttgatttgg taagcagcgg gcatttttcc
    10501 taacgaatat ctcagccaat agcctttatc gtggttaagg gtaatcaatc ttttactgac
    10561 ttttattggg tcaatattat tgtcggcagc ccatttacga atatcattat cggagatgtt
    10621 ttgcccaaca atataaatat cgagaggtgt ttgattattt gcgtaagaga ggattttccc
    10681 aagatcactt ttacaagaat cgcagttatc aaaacgggta aaataaacca cacgacctac
    10741 cgattgagaa atatgtggtt caacctcaaa aggtagctca ttcggataaa gtttctcaaa
    10801 ctcctgacga taaactttgt caaaatcaag ttcttttgac actcgttcat aatatttttt
    10861 cgccaacata cgagcatatt tttccctttc ctcagcagtt cgtgcttcga tacctaaagc
    10921 agttaacgga tctagatttg gcgaccacat acctcgctca cctttcatta attcggtata
    10981 acgactccat tcttcaacgg tcaatcccca ttctttagcc tgttttaggc tatcacttaa
    11041 ttgtgattgt tgatgttggc tttgtttctg caaagtcgat tgtgagctac ttacggtctc
    11101 tgtttgcata ttttgagcca gagtaggtaa tgcaaaggac aataaaagag gtagtactgc
    11161 caacggtata gctttacggc ataaattaaa cttcattgat atttccctca ttattcctca
    11221 atgacctcgg ttttaattgt tgtcgttgta acggcaacag caggtttatg agggttttgg
    11281 aatgaacttt cccgagcttt aaagcaaatc gtacgggata tatcattaag agttagttca
    11341 taagcgggac ctgcaatcat ttgtagagct tcatataatt tcatcggacc gaattggtaa
    11401 tgaacttttg gaagaggtcg ggtaaaaagt gtttgaaccg cattaccttg ttctccgata
    11461 ggtggataac acaaatctaa acccgtatta gttaatgtag aacgcaaacc ttgctggaca
    11521 ttcgcagtaa aacgattttt aggattggag actttgactg atacgacttg ttcaagcaga
    11581 tatttttgac cttcctccgg agcaatactg attaaggtgt agcgtccgtc acgtaccact
    11641 tcggtattcg aggtagtctg ttcttggtaa atatctttgg acacgatacg gtcttgtgga
    11701 ggtaattgtt gcatttgagg tgttagcaat gaatcaggca taggtgtgat ttcatcacta
    11761 ttttgagctg tatcctgttg atgaacacaa ccacttaagc caagcataaa aacagtcatt
    11821 aaggatattt ttgttttcat ttaatctctc caagtatgaa aggaatctag accaactatg
    11881 gcgaagtcat tgctgatttt gaatgattta ttctgttgtt gattgagaga ataaaaaaac
    11941 gccatcatta gacggcgttt gaaaacacaa taatgtgctg tgttaatcgg atttctcctt
    12001 agtattcata agaaaataag attgttgtac agctacgatc agcttctgtg atgatataaa
    12061 gactttcatc atctacatcg tagcgagcta caattctccc tccgtatttc attgcttcct
    12121 gattcgctaa tttatcttcc tcacacaaat tgccccaatc accataacag taacgattaa
    12181 gcaattcaac caaggcattt tgattgaaac attcagcaac agcacgagtt gtacatactc
    12241 gaccaagttc aaacgtcata catttctcct aaataaaata cggggaaatg tcacccatta
    12301 ggggagtatt tccccaagtg ggtggactaa gactgcggca agagtcgttt ttagtaaaaa
    12361 tttgcaaaaa attaggcagc taatttagcc ggcgtcattt ctttacgaat cttgtctaac
    12421 atttcattcc aatcatctat atcacagatt ctttggcgat taatgtaaaa atgcgtgtct
    12481 attgcattga tatagtagtc aaattcaact aaatctgaat cttgctcaat atctaattca
    12541 gtacagaatc gttgaattaa gcgagagatg acaagctcct gatacagatt accgaaatgt
    12601 ggtacagttt caaattcttt ggcaacaagc caaaagtctt caatactgac aatatcgcta
    12661 tattcttcta agatggcttc ccattgtcct aaatagcaag agatatcatc aaagtcaaaa
    12721 aaggaatctt ccattatgtt tctccaagtt ttaaataaaa agtggggaaa catacctacc
    12781 gagaatgttt cccgataggg tgtatagtca atattagcga taaatatcac cgctcactgt
    12841 gttaatttta cctaaaatag atttacaatg aatgtcagca ctcacagttt tcacattcga
    12901 ggttacatta ccatccactg tgacatcacc actgacagtt gaaacagatt tggcatcgcc
    12961 tgttacacga acattttctg aacctgcatt gaggttggtt acattgccaa atacttcaat
    13021 gttgatagtc ggagagggtt ctaatgcaat tgtttgtcca tcaacaatta atacattatt
    13081 gccggcagcc attgagatat aacctttatt tatcccaaca actgtttgca ctgtactatt
    13141 aatgatttga acgctgttga tctctttgcc tttagagtca aagacttgtt taccgttgat
    13201 ataaatacct tgtcgattca tataataatc ctctttgtat tagatgttaa aatgctcgat
    13261 agaatacgat attaccattg gaaatttcgt tgcgagcagc ctgtacaaac gtagttaatt
    13321 ttgctaaagc aggtgtatta gtttgctcta atcctactcg ctctaacata tctagatctt
    13381 ctatgttcaa taccaccgta gcatctttcc agaatgtctc aataggttga tagtattccg
    13441 gttccgatga ggctttaatt tcttttttga agaaatgaaa agccatctcg ttgtgaacta
    13501 agaaaatccc tctatcatcg gcttcagtcc aacggaaaaa ctccttggaa tgttcaatta
    13561 actgaatatc tgtaccataa atggggttat cccaatcaga cacaatgtta tattcacgct
    13621 ctaagcctaa cgaattaaaa cgttcaagag agattgaata cgctctagca agccaagaac
    13681 gttgagccca agatttttca gggattacct gtgccggatt ttttccgaac caatagcaat
    13741 cccaatctag gtaaatccaa tccttttcct ctatgctttt gtcaaaacca ataatagtaa
    13801 agccttgatc aagccattta ccatagccgt tctgaacaaa gacaacatcg ccaacaatac
    13861 catcaaaatg gcgaccatta aggactgttg gtgataatgt tttgacaaat tgttgaagtg
    13921 agttcatcgt gtttctccta aaattgtaaa ataaaggaaa acacaccgca gggagtgttt
    13981 ccctacggga cataaagtaa agcccaacat aaattgtcgg gcttgggtaa aattactcag
    14041 attgctcatc tgcttttggt ttttcttgat agacgatttc cccatcaatt ttaatcattt
    14101 tgatgcggat taagcgacct ttaagagaca ctccagtatc gccttttttg tgatatttac
    14161 tgtcacttga ataagtgaaa gtatcgaacc acaaatcagc cattacaaat gatacaagca
    14221 cttttttctc ttcagagacc gctttttggc attttttgat gagtgtttcg gtttctttac
    14281 cgactacatt catatcaaaa tatctgtact ctggtgagtc agattcgcca acaagagctg
    14341 caacacgaca agcataaaaa ggatttcctt ttttatgctc tactaaacga atatcgttta
    14401 aatagccaat gccggtagta tgtagattga agtaattttt ttgagtagaa gtttgagttg
    14461 tcatagtttg tttctccaaa tgtaaaaaag cggagaaaca tccttaccca acacgggaaa
    14521 aatgttcccc gcaaggtgga agtaaaatgg aatctcaatg aatattcact gaaatgatag
    14581 ggattgagtt ggcattcctg caaatttaca gagttccctt tcagaatgct gggagacatt
    14641 ttggcaataa ctcaactacc tctaaggata gccgtcgctt ctcaggagtt atgcgactta
    14701 ttaagtgctg ttatatacag cgtcttaaat tgag
//
LOCUS       DANLXS010000048        14733 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.048, whole genome shotgun sequence.
ACCESSION   DANLXS010000048 DANLXS010000000
VERSION     DANLXS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.048"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(1..489)
                     /gene="sixA"
                     /locus_tag="PXD71_001064"
     CDS             complement(1..489)
                     /gene="sixA"
                     /locus_tag="PXD71_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDL6291199.1"
                     /translation="MNIWIMRHGEAGFNASSDAARCLTDNGIKNTISQGKWLGEHLAN
                     QQIQLDKILVSPYLRAQQTLENVLIGMQAVNFPQHFTNLIESWDEVTPDGNPHTVESY
                     LHFLREEGAKNILLISHLPLVFDLVQILTNNQSNVQFPTSTIAEINWVKTKAELIRVK
                     HT"
     gene            616..2055
                     /gene="pyk"
                     /locus_tag="PXD71_001065"
     CDS             616..2055
                     /gene="pyk"
                     /locus_tag="PXD71_001065"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL6291200.1"
                     /translation="MSRKLRRTKIVCTMGPATDRGNTLEKIIAAGANMVRMNFSHGIP
                     EDHIERANKVREIAARLGRHVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPR
                     GEGHQEAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLEVEGVKVHTEVTVGGPLSNNK
                     GINKLGGGLSAPALTDKDKEDIKLAAKIGVDYLAVSFPQSSADLHYARELAKEAGLEA
                     KIVAKVERAETVATEEAMDDIILGADVVMVARGDLGVEIGDAALVGVQKRLIRRARKL
                     NRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGETANGDYPVETVKSM
                     AEVCLGAETMPSINISRHRMEGTFKSIEEAVAMSAMYTANHLEGVSAIISLTHSGETA
                     KLTSRISSGLPIYAMSRNQKALNLCALYRGVTPVYYNEDSRTMEGAKKAIAQLKEQGY
                     LLTGELVLLTHGDELKAGGTNTCRILRVE"
     gene            2151..3188
                     /locus_tag="PXD71_001066"
     CDS             2151..3188
                     /locus_tag="PXD71_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL6291201.1"
                     /translation="MHNKLLSICILRLSAIGDVCHTLAVIQAIQRKYPSAEITWIIGK
                     TEAYLMKDIPNVHLIPYDKKIGWKGIFTLWQKLSHKRFDFLLNLQTAFRASILSLGIK
                     AKKKIGFNQDRAREMQWLFTNQKVEMTDSPHVLDGQMMFAKAIGIMDLSAQWNLPVSD
                     EDLAYGKQFIDFTRKNILIAPCSSKKEKDWLPERYAEIANWLSKQNINVLIAGSPSQY
                     EMETAAKIQQLAPNCTSIAGKTTLKQLAGVIRQVDLVISSDSGPAHIATTQNTPVIGL
                     YAIHNPRRTGPYNDLDKVVSVYDEAILQSYGKPWQQLAWATKAKGKNWMEKITVESVK
                     QKVVETLKITL"
     gene            3228..4286
                     /locus_tag="PXD71_001067"
     CDS             3228..4286
                     /locus_tag="PXD71_001067"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL6291202.1"
                     /translation="MNTVFNQDSVHNVNIQDEKVLLTPQELKQEFPLPEHLRNQIERS
                     RKTISDIIHRRDKRQLIVIGPCSIHDPVAALEYGKKLKALSEQVSDKLYIVMRVYFEK
                     PRTTVGWKGLINDPKMDGSFDVETGLRIARKLCLDLAELGLPLATEALDPMTPQYLAD
                     LFSWSAIGARTTESQTHRELASGLSMAVGFKNGTDGDLAVALNAMQSSAQSHSFIGIN
                     QQGQVTLLKTKGNADGHIILRGGKTPNYEKQYVDDCEWVLKQANLPTAIMIDCSHGNS
                     NKDYRRQPLVAENVLEQLLAGNTSIIGLMIESNLHAGNQSSELPFEQMKYGVSITDAC
                     IDWQTTEELLTHFAEKLR"
     gene            4341..5462
                     /gene="tyrA"
                     /locus_tag="PXD71_001068"
     CDS             4341..5462
                     /gene="tyrA"
                     /locus_tag="PXD71_001068"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDL6291203.1"
                     /translation="MNPLAPLREQIDQVDQQLIALLSERLALVAEVGKVKSEHGIPVY
                     APERETAMISARRSEAEKQGIPADLIEDVLRRIMRESYANENKHGFKRVNSDIQKIVI
                     VGGKGKLGGLFGNYLTLSGYNVEALGRDDWDNAEQILANADVVIVCVPIANTLATIER
                     LQSYLTENMILADLTSVKHQPLQKMLEVHNGAVVGLHPMFGPDIASQAKQVVACCHGR
                     FPERYQWLLEQIKIWGAKIEEIEAAAHDNAMTYIQALRHFSTFAFGLHLSRQPVQLSQ
                     LLALSSPIYRLELAMIGRLFAQDGSLYADIIADKPENLATIETLKESFEQGLDFFKRN
                     DKAGFIKAFEEVHHWFGDYSEQFLKESRVLLQQAHDSRK"
     gene            complement(5653..6738)
                     /gene="recF"
                     /locus_tag="PXD71_001069"
     CDS             complement(5653..6738)
                     /gene="recF"
                     /locus_tag="PXD71_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL6291204.1"
                     /translation="MALTRLLINHFRNIQHTDLAFSPHFNFLVGANGSGKTSLLESIF
                     YLGHGRSFKSHISNRIIHYDKDDFVLHGKIDEAKHSWSVGIQKFRSGETTLKINGEDG
                     NKIADLAHLLPMQVITPEGLTLLNGGPSFRRAFLDWGLFHQHPDFYAHWNNLRRLLKQ
                     RNSALQQVRSYQELKAWDIELVKTTYAVSEMRAEYAEALRPEIEKTCQFFLPEIEIGL
                     SFHQGWEKGADYAEILAQGFERDKALGYTMIGAQKADFRFKANGLPVEDVLSRGQLKL
                     LMCALRLAQGEHLVAQKQRQCLFLIDDFASELDPTKRELLAHRLRESGSQVFVTAITQ
                     DQLNQMQWQDRSDDCLFTVKNGEIQPQ"
     gene            6878..7207
                     /locus_tag="PXD71_001070"
     CDS             6878..7207
                     /locus_tag="PXD71_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL6291205.1"
                     /translation="MFGKGGLGGLMKQAQQMQERMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRIEIDPSLMEDDKEMVEDLVAAAFNDAVRRAEEMQKEKMASVTAGMQLP
                     PGMKFPF"
     gene            7215..7820
                     /gene="recR"
                     /locus_tag="PXD71_001071"
     CDS             7215..7820
                     /gene="recR"
                     /locus_tag="PXD71_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL6291206.1"
                     /translation="MQTSPLLENLMEALRALPGVGPKSAQRMAYHLLQRNRSGGVALS
                     KALNEAMSHIGHCKSCRTFTEEEECTICKNPRRQMSGQLCVVEMPEDIQAIEQTGQFS
                     GRYFVLMGHLSPIDGIGPREIGLDLLQQRLEQESFHEVILATNPTIEGDATANYIAEM
                     CRMYNVKATRIAHGVPVGGSLEMVDGTTLSHSFAGRRDIDI"
     gene            7892..8218
                     /gene="grxD"
                     /locus_tag="PXD71_001072"
     CDS             7892..8218
                     /gene="grxD"
                     /locus_tag="PXD71_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HDL6291207.1"
                     /translation="METIDKIKQQISENPILLYMKGSPKFPSCGFSARAVEAVINCQV
                     PFGYVDILTNPDIRAELPKFANWPTFPQLWVEGELVGGCDIVLEMLQKGELQTLLKET
                     AAKHAA"
     gene            8360..9397
                     /locus_tag="PXD71_001073"
     CDS             8360..9397
                     /locus_tag="PXD71_001073"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL6291208.1"
                     /translation="MALKELDWANLGFSYIKTDYRFIAHWKDGKWNEGELTQDNTLHI
                     HEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAERMQRTADRLLMPRVPTELFIR
                     ACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKAAPEFIFSVFCCPVGAYFKG
                     GLAPSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAVEQSSTTRKFADAIYLDPK
                     THTKIEEVGAANFFGITKDNKFITPISESILPSITKYSLLHLAQERLGMEAIEGDVYI
                     DQLDQFAEAGACGTAAVITPVGGIQHKDKFHVFYSETEVGPVTRRLYAELTGIQFGDV
                     EAPQGWIVKVE"
     gene            9424..9972
                     /locus_tag="PXD71_001074"
     CDS             9424..9972
                     /locus_tag="PXD71_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HDL6291209.1"
                     /translation="MAKLFANSTACLYLENMKKLIFLGLSALALAGCSSTISNPNQLI
                     GQWQCTIEYDDFNIRTVDNLRFSTNGTMTNQGGIYYPIQKSIFEYSLQQNGRWSVKNG
                     AIVYRIVSESVQRSHRSQIWSELQRDAELQQFEENLFSSLSDSDNNKTVELIITDFDE
                     KEMDIKQEIKGHKAYKGQCIKQ"
     gene            9981..10325
                     /locus_tag="PXD71_001075"
     CDS             9981..10325
                     /locus_tag="PXD71_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL6291210.1"
                     /translation="MIQRFEVGSRFSEMAIHNGVAYLAGQVPLDDTADAYTQTQQVLA
                     EIDKWLAKANSDKSKILMATVYLKEMADYAEMNRAWDEWVAPNNAPPRAAVEAKLANP
                     NWKVEIVVTAAV"
     gene            complement(10358..12136)
                     /locus_tag="PXD71_001076"
     CDS             complement(10358..12136)
                     /locus_tag="PXD71_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDL6291211.1"
                     /translation="MSTLDFHFIHRFHQQVQALSHHPALRFMQDEKWSEISWLELQKQ
                     VNNLSYALLANGLAVQEKIAIFAQNMPRWTITDIAAMQIRAVAVPIYATSTTKQLEYI
                     LNDGDIKILFVGDQEQYDQAIEIANNCSLLIKIVAMKDSIDLRDFPIAQHWQDFIKVN
                     QDEAELTKRLNDKCLEDLFTLIYTSGTTGEPKGVMLDYANLAHQLQAHNKALPHIDEN
                     DISLSFLPLSHIFERAWVTYVLYRGAVNCYLEDPQQVRQALSTVRPTLMCAVPRFYEK
                     IYTAVWDKVQQAPIHRRALFKWAIRTAEDNLFGKKSAFFHRLQYIMADKLVLGKLRAL
                     LGGRIRMMPCGGAKLDPSIGLFFQSIGINVKLGYGMTETTATISCWQEKGFNINSTGT
                     LMPDVEVKISEENEILVRGGLVMKGYYKKPEATAQAFTAEGFLKTGDAGELDSEGNLY
                     ITDRIKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEE
                     YAKKLNIRYQDRLELIKHYEVVRMFEQRINELQKDLASFEQIKKFTLLSQAFSTKMEE
                     ITPTLKLRRKVILERYKQQIEKMYES"
     gene            complement(12362..12931)
                     /locus_tag="PXD71_001077"
     CDS             complement(12362..12931)
                     /locus_tag="PXD71_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDL6291212.1"
                     /translation="MNQENHKIEDIIRIFNQCFSDEYNTRLERGGNYPIYLPEFMDED
                     GVPSERPYNVILFAHGYYSSALHEIAHWLVAGEARRKLEDFGYWYEPDGRSAERQREF
                     ESAEIKPQAIEWVLATAAGFRYFASADNLSGNPGDNSAFKQAVYDQVKLYAERGYLPK
                     RAETLRKALCAFYGTPDVIDLAQFDIERI"
     gene            complement(12941..14704)
                     /locus_tag="PXD71_001078"
     CDS             complement(12941..14704)
                     /locus_tag="PXD71_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDL6291213.1"
                     /translation="MIRYFFSLVLSLLMINSSHANLFSQPKFLKSEEAFVFSIQNENE
                     TLQLNWDIADNYYLYKKEIRVTPQNIEIGDITFPAAEQYNDEFFGQVEIYRNHLGLKV
                     PFKEIKDNASIVVEYQGCTKGFCYPPERVELKLDSILSATPEELAQLEAQAVEKSENF
                     AKTDNMAPPENVAKAEQDQLAENLANNRFSIFWFFVLGVGLAFTPCVLPMLPLLSAIV
                     IGNKQRPSTAKALLLSIAYVQGMAVTYTLLGLIVAAIGLPFQIALQSPPVLIGLSIVF
                     ILLACSMFGLYEIQLPNSWQQKLNAMSQKQQGGALGSVFLMGMIAGLIASPCTSAPLS
                     GALLYVAQSGDLVTGGLALYLLALGMGLPLILITLFGNKILPKSGEWLLKVKAAFGFV
                     MLALPVFLLSRVLPSHYEPFLWSALAVAFLIWLVDAIPSNSITKKIIKVLLLLALAIA
                     AKPWADLAWNGSTTQQQTTPHLALEKIQSLADLETKLTASKGKKVMLDLYADWCVACK
                     EFEKYTFSDQNVQNKLNEMVVLQIDMTKNSAENIELMKHFNVLGLPTILFFDENGNEM
                     SQSRVTGFLDAEQFLAWLNKL"
BASE COUNT         4425 a         2846 c         3194 g         4268 t
ORIGIN      
        1 ttaagtatgc tttactcgaa ttagctcagc ttttgtcttt acccaattaa tttctgctat
       61 ggttgaagtt ggaaattgta cattgctttg gttattcgtt aaaatttgga cgagatcgaa
      121 cacaagtggg agatgtgaaa tcagaagaat attttttgcg ccttcctctc ttaaaaaatg
      181 tagatagctt tctactgtat gcgggtttcc gtcaggcgta acctcgtccc aactttcaat
      241 gaggttggta aaatgttggg gaaaattgac cgcttgcata ccaataagca cattttcaag
      301 cgtttgttgc gccctaaggt atgggctgac gagaatttta tctaattgaa tttgttggtt
      361 ggctaaatgt tctcctaacc attttccctg tgaaatggtg ttttttatac cattgtcggt
      421 taaacagcga gcagcatcac tcgaagcatt aaagcctgct tccccatggc gcataatcca
      481 aatattcata acattctcct ttagttgtat ttaaactctc ttgatttagg cgtataatga
      541 ccctcgttta acgcataatc aagatgctta gaaagatttt aacttaattt ttaaatttat
      601 tgatggagtc aaataatgtc tagaaaactc agaagaacga agatcgtttg tacaatggga
      661 cctgcaacag atcgtggcaa tacgttggaa aaaatcattg ctgccggtgc aaatatggtt
      721 cgtatgaatt tctctcacgg tattcctgaa gatcacatcg aacgtgcgaa taaagtgcgt
      781 gaaattgctg caagattagg tcgccacgtt gcgattttag gcgatttgca aggtcctaaa
      841 attcgtgttt cgacatttaa agacggtaaa attttcttaa atattggtga taaattcacc
      901 ttagacgcag atttaccgcg tggagaaggc catcaagaag ctgtaggttt agattacaaa
      961 aacttaccaa atgatgtagt acctggtgat attctattgt tagatgacgg taatgttcaa
     1021 ctgaaagtgt tggaagtgga aggcgttaaa gttcatactg aagtgacagt tggtggcccg
     1081 ttatcaaaca ataaagggat taacaaatta ggtggtggtt tatcagctcc tgcattaacc
     1141 gacaaagata aagaagacat taaattagcg gcgaaaatcg gtgtggatta cctagcggtt
     1201 tcattccctc aatcaagtgc agatttacat tatgcacgtg aattagcaaa agaagcaggc
     1261 ttagaagcga aaattgtagc gaaggttgaa cgtgccgaaa ctgttgcgac tgaagaagca
     1321 atggacgaca tcattttagg tgcggacgtt gtgatggttg ctcgtggtga cttaggggtt
     1381 gaaattggtg atgcggcttt agtgggtgtg caaaaacgtt taatccgccg tgctcgtaag
     1441 ctaaatcgtg tggtaattac cgcaacccaa atgatggaat cgatgattaa aaaaccaatg
     1501 ccgacccgtg ctgaagtgat ggatgttgca aatgctgtgt tagatggtac tgatgcggta
     1561 atgctttccg gcgaaactgc aaacggtgac taccctgttg aaacggtaaa atcaatggca
     1621 gaagtgtgct taggtgctga aacaatgcca agcattaaca tttctcgcca ccgtatggaa
     1681 ggtacgttta aatctatcga agaagctgtg gcaatgtctg caatgtacac cgcaaaccat
     1741 ttagaaggtg tatcggcaat tatttcatta actcattcag gcgaaacggc taaattaacc
     1801 agccgtatca gctcaggttt accgatttat gcgatgtctc gtaaccaaaa agcattaaac
     1861 ctttgtgcgt tatatcgtgg tgtaacaccg gtatattaca atgaagacag ccgcacaatg
     1921 gaaggggcga aaaaagccat tgctcaatta aaagaacaag gctatttatt aaccggcgaa
     1981 ttagtgcttt taactcacgg agatgaatta aaagctggcg gtacaaatac ttgtcgtatc
     2041 ttaagagttg aataatctaa acgattaacg caaaagccac ataaaacact ttatgtggct
     2101 ttttttctgt cttaatatag cttaatgtaa tctgaactat tgatattatt atgcacaata
     2161 aacttctttc tatctgtatt ctccgccttt ctgccattgg cgacgtttgc cataccttag
     2221 cggttattca agctattcaa cgaaaatatc ctagtgctga aattacttgg attatcggca
     2281 aaacggaagc gtatttaatg aaagatattc cgaatgttca cttaattcct tatgataaaa
     2341 aaatagggtg gaaaggtatc tttacacttt ggcaaaaact ttctcacaag cggtttgatt
     2401 ttttattaaa tttacaaacg gcattccgag cttcaattct atcgcttggg attaaagcaa
     2461 agaaaaaaat cggctttaat caagacagag caagggaaat gcaatggttg tttaccaacc
     2521 agaaagtaga aatgacagac tctcctcacg tgcttgacgg gcaaatgatg tttgccaaag
     2581 ccattgggat aatggattta tcggcacaat ggaatttacc tgtgagtgat gaagatttag
     2641 cttatggcaa acaatttatt gattttacaa ggaaaaatat actcattgct ccttgttcaa
     2701 gcaagaaaga gaaagattgg ttgccggaac gctatgcaga aattgctaac tggctaagta
     2761 agcaaaatat caatgtatta attgcaggtt ctccatcgca atatgaaatg gaaaccgcag
     2821 ccaaaattca acaacttgcc ccgaattgta cgagtattgc cggtaaaacc acgctaaaac
     2881 agcttgcagg tgttattcgt caggttgatt tggtgatctc ttcagattca ggtcctgccc
     2941 atatcgccac tacgcaaaat acgccggtga ttgggttgta tgcgattcat aatccaagac
     3001 ggacaggccc ttacaatgac ttagacaaag tggtttcggt atatgatgaa gcgattttgc
     3061 aatcttatgg taaaccttgg cagcaattag cttgggcaac taaagctaaa ggcaaaaatt
     3121 ggatggaaaa aatcacggtg gagagtgtga aacaaaaagt agttgaaact ttaaaaatta
     3181 cgttgtaaag tttgtgtatt aaaagattta cacaaaagga aattaaaatg aacacagtgt
     3241 ttaatcaaga tagcgtgcat aacgtaaata tccaagatga aaaggtatta ctgaccccac
     3301 aagagttaaa acaagaattt ccgttgccgg aacatttacg caatcaaatt gaacgttcac
     3361 gcaaaacgat ttccgatatt attcacagac gagataaacg ccaactgatt gtgattggac
     3421 cttgttcgat tcacgaccca gttgctgccc ttgaatatgg taaaaaatta aaagctcttt
     3481 cggagcaagt gagtgataag ttgtatattg tgatgcgggt ttattttgaa aagccccgca
     3541 ctactgtagg ttggaaaggg ctgattaacg atccgaaaat ggacggctct tttgatgtcg
     3601 aaaccggctt acgtatcgca cgaaaactct gtttggattt agccgaactt ggcttgccgt
     3661 tagcgaccga agccttagat ccgatgacac cacaatattt agcggatttg ttcagctggt
     3721 cggcaattgg agcaagaaca actgagtctc aaactcaccg tgaattagcc tcaggtttgt
     3781 caatggcggt aggctttaaa aatggtacag atggtgattt agccgttgca cttaatgcga
     3841 tgcaatcttc cgctcaaagc catagcttta tcggcattaa ccaacagggg caagttactt
     3901 tactcaaaac aaaaggtaat gcagacggac atattatttt acgtggcggt aaaaccccaa
     3961 attatgaaaa gcaatatgtt gatgattgtg aatgggtgct aaaacaagca aacttaccta
     4021 cagcgattat gattgattgc agccacggca attccaataa agattaccgc cgccaaccgc
     4081 tggtggcaga aaatgtatta gagcagctac ttgctggcaa tacttcgatt atcggtttaa
     4141 tgattgaaag caatttacac gcaggtaatc aatcatcaga attgccgttc gagcagatga
     4201 aatacggtgt ttctattacg gatgcttgta ttgactggca aactacagaa gagcttttaa
     4261 ctcactttgc tgaaaagttg cgttaaagat aatttgcaaa aaattgcata aaaacgaccg
     4321 cttgtaaatt ggtaacaaat atgaacccat tagccccatt acgagaacaa attgatcaag
     4381 ttgatcaaca acttatcgcc ttactttctg aacgcttagc tcttgtggca gaagttggga
     4441 aagtcaaatc agaacacggc attcctgttt atgctccgga gcgagaaacc gcaatgattt
     4501 ccgcccgccg ctctgaagcg gaaaaacaag gtattccggc ggatctcatt gaagatgtgc
     4561 tacgccgtat aatgcgtgaa tcttatgcca atgaaaacaa acacggcttc aaaagagtta
     4621 attcggacat tcaaaaaatc gtgattgttg gtggtaaagg gaaattaggt gggctgtttg
     4681 gcaattattt aaccctatcc ggctataatg ttgaggcatt aggacgtgat gattgggaca
     4741 atgccgagca gattttagcc aatgcagatg tggttattgt gtgtgtgccg attgccaaca
     4801 ccctagcaac catcgagcgt ttacaatctt atttaaccga aaatatgatt ttggcggatt
     4861 taacctccgt caaacatcag ccgttgcaaa aaatgctgga agtgcataat ggagcggtag
     4921 tcggtttaca cccgatgttc ggaccggata ttgcaagtca ggcaaagcaa gtggtcgctt
     4981 gctgccacgg gcgtttccct gaacgttatc aatggttgct tgagcaaatc aaaatttggg
     5041 gggcaaaaat tgaagagatt gaggcagcag cacacgataa tgcaatgacc tacattcaag
     5101 ccctacgcca cttttccact tttgcattcg ggctacatct ttctcgccag ccggtgcaac
     5161 tctctcaatt attggcacta tcttcgccga tttaccgctt agagttggca atgattgggc
     5221 gtttatttgc ccaagacggc agcttatatg ccgatattat tgccgataaa ccggaaaatt
     5281 tagccaccat cgaaacctta aaagagagtt ttgaacaagg gctagatttc tttaaacgca
     5341 acgacaaagc cggttttatc aaggcttttg aggaagtgca tcactggttt ggcgattatt
     5401 ccgagcagtt cttgaaagag agtagagtat tgctccaaca ggctcacgat tcaagaaagt
     5461 aaaggtaggt tgaggttttg ctctgatagc actagcgtcc acgctagtgc tatttaatat
     5521 tctacaagcg gttaataaag agctgttttt tgcaaatttt tatgcaaaat tatccgcttg
     5581 ttttggcacg agcgtggacg ctcgcgccat cggaagaaac atttttgtaa aattgccccc
     5641 aaatctgacc gcttactgtg gctgaatttc tccatttttt accgtaaaca aacaatcatc
     5701 gcttctgtct tgccattgca tttgattgag ctgatcttga gtaatggcgg taacgaatac
     5761 ttgtgagcca ctttctctta aacgatgtgc taaaagctca cgtttggttg ggtcgagttc
     5821 ggaggcaaaa tcgtcaatta aaaataggca ttggcgttgt ttttgggcga ccaaatgttc
     5881 gccttgagcc agtcttaacg cacacatcaa tagctttaat tgcccacggg aaagcacatc
     5941 ttccaccggt aagccgttcg ctttaaaacg gaaatcggct ttttgagctc caatcatcgt
     6001 atagccaaga gctttatccc gctcaaaacc ttgtgccaga atctcggcat aatccgcccc
     6061 tttttcccag ccttgatgaa agctcaagcc gatttcgatt tccggcagga aaaattggca
     6121 ggttttctca atttcggggc gtaacgcttc ggcgtattcg gctcgcattt cgctcacggc
     6181 ataagtggtt ttcaccaatt cgatatccca cgctttgagt tcttgataag agcggacttg
     6241 ttgcaaagcc gaattacgtt gctttaataa acggcgaaga ttgttccaat gagcgtagaa
     6301 atcagggtgc tggtgaaata agccccagtc caaaaaagca cggcgaaagc tcggaccacc
     6361 gttaagtaag gttaagcctt cgggggtgat cacttgcatc ggcaataaat gggctaaatc
     6421 ggcaattttg ttgccgtctt caccgttgat tttaagtgtc gtctcgccac tgcgaaattt
     6481 ctgaatgccg accgaccaac tgtgctttgc ctcgtcaatt ttaccgtgta gtacgaaatc
     6541 gtctttatcg taatggataa tgcggttgct gatatggctt ttgaatgaac gcccgtgtcc
     6601 aagatagaaa attgattcca acaaactggt tttgccactt ccgttcgccc cgactaaaaa
     6661 attaaaatgc gggctaaatg ctaaatcggt atgttggata tttctaaaat ggttgattaa
     6721 taaacgggtt aaagccatag ttaaagtgcg aggaataaaa agagtagcca ttttagcaaa
     6781 tttgcaaaat tttgggaaaa attaaccgct tgcggattag aaagtggttc agtagaataa
     6841 gccgtttatt ttttattgaa aattaagagg aaaaattatg tttggaaaag gtggcttagg
     6901 cggcttaatg aaacaggctc agcaaatgca agagcgtatg caaaaaatgc aagaagagat
     6961 cgcccaatta gaagtaacag gcgagtctgg tgcgggtttg gtaaaagtca ctattaacgg
     7021 ggctcacaat tgccgccgaa ttgaaattga tccatcatta atggaagatg ataaagagat
     7081 ggtggaagat ttagttgccg ctgcctttaa cgatgcggtt cgccgtgcag aagaaatgca
     7141 aaaagagaaa atggcaagtg tgactgccgg tatgcaactg ccgccgggta tgaaattccc
     7201 attctaattt aagtatgcaa accagtcctt tacttgaaaa tttgatggaa gccttgcgtg
     7261 cattgcccgg tgtcggaccg aaatcggcac agcgaatggc ttaccatctg ttgcaacgta
     7321 atcgttccgg cggtgtggcg ttgtcaaaag ccttaaatga ggcaatgagc catatcggac
     7381 attgcaaatc gtgccgtact tttaccgaag aggaagagtg tacgatttgt aaaaatcctc
     7441 gccgtcagat gagcgggcaa ctttgtgtgg tagaaatgcc ggaagacatt caagcgattg
     7501 agcaaaccgg gcaattttca gggcgttatt ttgtgctgat ggggcatttg tcgccgattg
     7561 atggtatagg ccctcgtgaa atcggcttag atttattgca acaacgcttg gaacaggaat
     7621 ctttccacga agtgattttg gcgactaatc cgaccattga aggtgatgct accgcaaact
     7681 acattgctga aatgtgcaga atgtataacg tgaaagctac tcgtattgct cacggtgtgc
     7741 cggtcggtgg ttcattagaa atggttgatg gcacaacgct ttctcactct tttgcaggac
     7801 ggcgtgatat tgatatttaa agctatttct gtgctaactt aattctgata taattactca
     7861 actattttaa attattattt tgaggctaaa aatggaaact attgacaaaa tcaaacaaca
     7921 aatcagcgaa aatccgattc tactttatat gaaaggttca ccgaaattcc catcttgtgg
     7981 tttctccgct cgtgcggtgg aagcggtaat taactgccaa gtgcctttcg gttatgtgga
     8041 tattttaacc aatccggata ttcgtgcgga attgccaaaa tttgcaaact ggccgacttt
     8101 cccacaatta tgggtagaag gtgaattagt tggcggttgt gacatcgtat tagaaatgct
     8161 ccaaaaaggc gaacttcaaa ccttattaaa agaaaccgca gcaaaacacg cagcataatt
     8221 tcaaaccagt atgttaaggc tcttatgttt aagagcctta attttttctt gcgatttagg
     8281 cttaaacttc ttacctcaag agctattttg ttttagaatg tcaaaattat ttcgtaacat
     8341 aaaaataaaa ggacacaaca tggcattaaa agagttagat tgggcaaatt taggcttttc
     8401 ttatatcaaa accgattacc gtttcattgc acattggaaa gacggaaaat ggaatgaagg
     8461 cgaattaact caagacaata cactgcatat tcacgaaggc tcaactgccc tgcactatgg
     8521 gcaacaatgt tttgaggggt taaaagccta tcgctgtaaa gatggctcaa ttaacttatt
     8581 ccgcccggat caaaatgccg agcgtatgca acgcaccgca gatcgtttat taatgccacg
     8641 tgtgccaacc gagctgttta ttcgtgcctg taaaaaagtg gtaaaagcca atcaagattg
     8701 gttagggcct tacggttcgg gggcaaccct ttatttacgt ccgtttttaa tcggcgtggg
     8761 cgaaaatatt ggggtgaaag ctgcacctga atttattttt tccgtattct gctgtccggt
     8821 gggggcatat ttcaaaggtg gcttagcacc ctcaaacttc attacaaccg actatgaccg
     8881 tgcagcccca atgggaacag gtggtgtgaa agtaggcggt aactacgctg cgagtttatt
     8941 accgcacgaa ttggcggttg agcaaagttc aactacccgt aaatttgccg atgcgattta
     9001 tttagatccg aaaacccata ccaaaattga agaagtgggg gcggcgaact tctttgggat
     9061 taccaaagac aacaaattca tcaccccgat ttcagagtcg attttgccaa gtattaccaa
     9121 atactcattg ctacaccttg ctcaagagcg tttaggtatg gaagcgattg agggagatgt
     9181 gtatatcgac cagttagacc aatttgccga agccggtgct tgtggtacgg ctgcggtgat
     9241 tacgccggtt ggtggtattc aacataaaga caaattccac gtcttctatt cagaaaccga
     9301 agtggggcct gtcacccgcc gtttatacgc agaattaacc ggtattcaat ttggcgatgt
     9361 tgaagcaccg caaggctgga ttgtaaaagt agagtaattt tacacaagta acaagcggtc
     9421 gaattggcaa aactttttgc aaattcaacc gcttgtcttt atttagagaa tatgaaaaag
     9481 ttaatttttt taggtttatc agcacttgca ttagctggtt gttcctcaac aatttctaat
     9541 cctaatcaac tgattgggca atggcaatgc actattgagt acgatgattt taatattcgt
     9601 acggtggata atttacgatt ttccacaaat gggactatga ccaatcaagg tggtatatat
     9661 taccctattc aaaagtcgat ttttgagtat tcattacaac agaatgggcg ctggagtgtg
     9721 aaaaacgggg ctattgttta tcgtatcgtc tcagaatctg tacagcgtag ccatcgttca
     9781 caaatatggt ctgaattaca acgtgatgcg gagctacaac aatttgaaga gaatttattt
     9841 agctcacttt ctgatagtga taataataaa acagtggaac taatcatcac tgattttgat
     9901 gaaaaagaaa tggatatcaa gcaagaaata aaagggcata aagcttataa agggcaatgt
     9961 ataaaacagt agaggtaaaa atgattcaac gttttgaagt aggtagccgt ttttcggaaa
    10021 tggcaataca taacggtgtg gcttacttgg cagggcaagt gccgttagat gacaccgccg
    10081 atgcttacac ccaaacccag caagtgcttg cagaaattga taaatggctg gcaaaagcca
    10141 acagcgataa aagtaaaatt ctaatggcta cggtttattt aaaagagatg gcagattatg
    10201 cggaaatgaa ccgtgcgtgg gacgaatggg ttgccccaaa taacgctcca ccaagagcgg
    10261 cggtggaggc taaattagcc aatccaaatt ggaaagtcga aattgtggtt accgctgcgg
    10321 tataaatgaa aagcagaaag acgatctctg tttttcatta tgattcgtac attttttcga
    10381 tttgttgctt atagcgttct agaatgacct ttcgtcttaa ttttaatgta ggcgtaattt
    10441 cttccatttt ggtgctaaat gcttgcgaaa gtagtgtaaa ctttttgatc tgttcaaaac
    10501 ttgcaaggtc tttttgtaat tcgttaatgc gttgttcaaa cattctgaca acctcataat
    10561 gtttaatgag ttctaatctg tcttgatagc gaatattgag cttttttgca tactcttcga
    10621 ggctatcaaa gcaaggaact atcaacgcag aaacatattt tttggcatca gcaatcaccg
    10681 caatttgctc aataaactta tctttaccta tttttccttc aatgtattgt ggggcaatat
    10741 atttaccgtt tgaagttttc attaattctt taatacgatc ggtaatataa agattgcctt
    10801 ccgaatccag ttcaccggca tctcccgttt ttagaaaacc ttcagcagta aaggcttgag
    10861 cagtggcttc cggttttttg tagtaacctt tcataaccaa gcctccacgc actaaaattt
    10921 cattttcttc actaattttg acctcaacat caggcattaa ggtaccggtt gagttgatat
    10981 taaagccttt ttcctgccag catgaaatag tggcagtagt ttcggtcatt ccgtatccaa
    11041 gttttacatt gatgcctata ctttggaaaa atagcccgat agacggatct aatttagctc
    11101 ctccacacgg catcatacga atacgaccgc ctagtaaggc tcgtaatttt cctagcacca
    11161 atttatctgc cataatgtac tgcaagcggt ggaaaaaggc tgatttttta ccaaacagat
    11221 tgtcttcggc ggtacggatt gcccatttaa ataacgctcg gcgatgtatc ggtgcttgtt
    11281 ggactttatc ccacaccgca gtatagattt tttcatagaa ccttggtacg gcacacatta
    11341 atgtaggtcg taccgttgag agtgcttgac gtacctgttg aggatcttcc agatagcaat
    11401 ttactgcccc tctgtatagc acataagtga cccaagcacg ttcaaagata tgcgataaag
    11461 gtaaaaatga aagggatata tcattttcgt caatatgagg gagtgcttta ttatgtgcct
    11521 gaagttgatg agctaaatta gcataatcta acataacacc tttaggctca ccggttgttc
    11581 ccgaggtata aattaaagtg aataaatcct ctaagcattt atcatttaaa cgtttggtta
    11641 attcagcttc atcttgatta accttaatga aatcttgcca atgttgagcg atagggaaat
    11701 cacgcagatc tattgagtct ttcatcgcga caatttttat taatagcgaa cagttgttcg
    11761 ctatttctat ggcttgatca tattgttcct gatcgccaac aaagaggatt tttatatccc
    11821 cgtcattcag gatatattca agttgtttgg tggtactggt tgcataaatc ggcactgcta
    11881 ccgcacgaat ttgcattgca gcaatatcgg taattgtcca acgaggcata ttttgtgcaa
    11941 aaatagcgat tttctcttga acggcaagac cgtttgccag caaggcataa gaaagattat
    12001 tgacctgttt ttgtaattct aaccaactta tttctgacca tttttcatct tgcataaaac
    12061 gtaaagctgg gtggtggcta agagcctgaa cttgctggtg gaaacggtgg ataaagtgaa
    12121 aatctaaagt gctcataatc ttatttttac tttctatttc agtgaacagt tgtacgctaa
    12181 tataatagaa aatatgaaaa aaagaaacag ttgtactctg aaacaaaggg ttttggatag
    12241 gaagtaatca aggcaataga aatagataag tagggtatcg tttagtacaa atgcaatatt
    12301 tttcaaaaat gagaccgctt gtatatgcaa caagcggtct gttttgttaa aaattttgca
    12361 actaaattcg ttcaatatca aattgagcta aatcaatcac atcaggtgta ccgtaaaaag
    12421 cacagagggc tttacgcaag gtttcagctc gtttgggaag gtagccacgt tctgcataga
    12481 gcttgacttg gtcgtacact gcttgtttaa aggctgaatt atcgccggga ttgcccgaga
    12541 gattgtcggc actagcaaaa taacgaaaac cggcagcagt tgctaacacc cattcaatcg
    12601 cttgcggttt gatttccgca ctttcaaatt cacgttggcg ttcggcagag cgtccgtccg
    12661 gctcgtacca atagccaaaa tcttcaagtt tacgccgagc ttctccagca acgagccaat
    12721 gggcaatttc gtgcaaggca ctgctgtaat agccgtgagc aaataaaatc acattgtaag
    12781 ggcgttcgct cggcacacca tcttcgtcca taaattcggg caagtaaatc ggataattac
    12841 ccccccgctc taggcgagtg ttatattcat cagaaaagca ttgattaaaa atgcgaataa
    12901 tgtcttcaat tttatgattt tcttgattca tttcttttct ttataactta ttcagccaag
    12961 ctaaaaattg ctcggcatcc aaaaagccag taacacgaga ttggctcatt tcattgccgt
    13021 tttcatcaaa aaacagaatc gttggcaaac ctaatacatt aaagtgtttc atcagctcga
    13081 tattttcagc cgagtttttc gtcatatcga tttgcaacac taccatttca ttcagtttgt
    13141 tctgaacatt ttggtcacta aaggtgtatt tttcaaactc tttgcaggcg acacaccagt
    13201 cggcatagag atcgagcatc acttttttac ctttggaagc ggtcaatttt gtctctaaat
    13261 cggcaagtga ttgaattttt tctaacgcta agtgcggtgt agtttgctgt tgagtggttg
    13321 agccgttcca agctaaatct gcccaaggtt tagcggcaat ggctaaagcg agcaataata
    13381 agactttgat gatctttttc gtgattgaat tagatggaat cgcatccacc aaccagatca
    13441 aaaatgcaac cgcaagggcc gaccataaaa acggttcata gtggcttggc agcacacggc
    13501 ttaataagaa caccggtaag gcaagcataa caaaaccgaa tgcagctttc acttttaaca
    13561 gccattcgcc ggattttggc agaattttgt tgccgaataa ggtaatcaaa atcagcggta
    13621 agcccattcc cagtgcgagt agatagaggg ctaagccacc ggtgactaaa tcgccacttt
    13681 gtgccacata aagcaacgca ccggaaagtg gggcagaagt acaaggggaa gcaattaagc
    13741 ctgcgatcat ccccattaaa aacacgctac ccaacgcacc accttgctgt ttttggctca
    13801 ttgcattgag tttttgttgc cagctatttg gtagctggat ttcgtataag ccaaacattg
    13861 agcaggcaag cagaataaag acgatagata aaccaatcaa caccggtggg ctttgtaagg
    13921 cgatttggaa aggcaagccg atagccgcta caattaagcc aagtaaggtg taagtaactg
    13981 ccattccttg cacataggca atacttaaca gcaatgcttt agcggtagaa gggcgttgct
    14041 tatttccaat tacaatcgca gagagaagtg gtagcatcgg cagtacgcaa ggagtgaatg
    14101 ctaatccaac gcctaataca aaaaaccaaa aaatggaaaa acgattgtta gctaaatttt
    14161 cagccagttg atcttgctct gcttttgcca cattttcagg gggcgccata ttgtcggttt
    14221 ttgcaaaatt ttcgctcttt tcgaccgctt gtgcttccag ttgcgccagt tcttcaggtg
    14281 tagcagagag aatactgtct aatttaagct caacccgttc cggtggatag caaaagcctt
    14341 tggtacaccc ttgatattcc actacgattg aggcgttgtc tttaatttct ttaaacggta
    14401 cctttagccc taagtggttg cgatagattt ccacctgccc gaaaaattca tcgttatatt
    14461 gctcggcagc gggaaaagtg atgtcgccta tctcaatatt ctgtggcgtg acacggattt
    14521 cttttttata aagataataa ttgtctgcaa tatcccaatt taactgtaat gtttcatttt
    14581 cattttggat tgaaaaaaca aaggcttctt ccgattttag gaatttgggt tgagagaaaa
    14641 gatttgcgtg agatgagttt atcatcaata aactcaagac gagtgaaaaa aagtatctaa
    14701 tcatattgaa atcctatttt taattgtctc ggc
//
LOCUS       DANLXS010000049        14641 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.049, whole genome shotgun sequence.
ACCESSION   DANLXS010000049 DANLXS010000000
VERSION     DANLXS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14641
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.049"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            2..214
                     /locus_tag="PXD71_001079"
     CDS             2..214
                     /locus_tag="PXD71_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6291214.1"
                     /translation="MTNKKIQKAIELVGTQTKLAEACGVSQPTVLKWLNGGGISAEYL
                     LKIEAATNGQVTIREICEELGNATAK"
     gene            complement(313..558)
                     /locus_tag="PXD71_001080"
     CDS             complement(313..558)
                     /locus_tag="PXD71_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291215.1"
                     /translation="MNTEDQIIELTVEVENLRHQLNAKNIILDDLKGRLTDYQRKRLD
                     RLFERSRRQYLNDLDLEEEQAEALEAICNELESFLAN"
     gene            691..963
                     /locus_tag="PXD71_001081"
     CDS             691..963
                     /locus_tag="PXD71_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291216.1"
                     /translation="MNLDHTCTNCKSKNLRVRTSEKIGLLTIQAVTYCNNCGTEQLVQ
                     SQIIRVRTPIYKERPEAMRINKCLMEADLNTPDLFDGVVADQPKQD"
     gene            1046..1378
                     /locus_tag="PXD71_001082"
     CDS             1046..1378
                     /locus_tag="PXD71_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291217.1"
                     /translation="MSKQLYYRHKSRWNRLNRLKKMNKRRLNLFLQEKRVANLESRLA
                     DEVELAKLNSEQIGDLLQALELKVNRLIRQNEKLKARVIKLEKVKQTQKQGLFAMFKK
                     VLGSKGNP"
     gene            1391..1684
                     /locus_tag="PXD71_001083"
     CDS             1391..1684
                     /locus_tag="PXD71_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291218.1"
                     /translation="MDFVATYQKIRINTNHKQFTCNHLGEIWGKENDDFKRFLLRVAF
                     LQDNPEPKPWGAYSPQEKRLLHSFSLWLEQIADFQRVMKRESKLREKHINAPD"
     gene            1836..2078
                     /locus_tag="PXD71_001084"
     CDS             1836..2078
                     /locus_tag="PXD71_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291219.1"
                     /translation="MSLITVNDYKIKKDILDADGKPCYQLYQGEELLRDTTNLSEIIA
                     YMFIRAEDKPFDELYNHIINQEAGAKTDIKFTVTVR"
     gene            2075..2407
                     /locus_tag="PXD71_001085"
     CDS             2075..2407
                     /locus_tag="PXD71_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291220.1"
                     /translation="MSQITKSAYTHIHPNWQAPRGNTYRIERYELKRRYALFCNGERV
                     VLYKDPTHPNKNVESHIESDVLLLWLLKNELGWSGNIKEPLSPQDVENIRLLEQAFCL
                     TYNGTFIK"
     gene            2404..4770
                     /locus_tag="PXD71_001086"
     CDS             2404..4770
                     /locus_tag="PXD71_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDL6291221.1"
                     /translation="MNYQNEILSATEAFAIGVSLPPVAGASACALELHSDRLYQAFKQ
                     PYTSMVQQELFSKDPIHEPLRQSYFERLPRALAEHFGRQYKQKLHYQSQYDTADWFKA
                     EMARKMPRIEAVISQYCDVFDFLKQSHQDLDFLAELDSNVYFGAGVDTQQAVQKIKQD
                     AEVKGIQLPMHLCRLENSSRYLKAHGIRPLAYQTEEQIKQLALGIAYRVRQIQQDNIE
                     QNMHKATDGDTAYSVLLECYRAMMGEVNKLKIDSPYQKKARKGRLTEEEITTGFLKMT
                     CEKWWTRKLSKIAEQMKEHLAIACGMVNMLSPYCSNARLKAFEAQRKANIDYLKSMII
                     ANIAEPEEQLSLFETWLKSASNPKIKRLELLTRMNGFERYADKQGHEGWFITLTAPSK
                     YHAMLSRTSSVNPKWNGASPAETQVYLVNTWAKIRAKLNREGVMAYGFRVAEPHADAT
                     PHWHLILFTRPEDMEKLRRVFLSYALEVDGTEAGAKKYRCKFKRIEKEKGSATGYLVK
                     YVSKNIDGFGMDGELSDEANIQAKENAARVGAWSSVWCIRQFQQLGNIPISLWRELRR
                     LGSVEQEDETLEKLRVIADSGEWDVFTEELGGALVKRADLVARITYTERKSETGEALY
                     TMYQEPSLKVSGIINIKNGVQINTRPKEWSIQLKPKNWEEQQLQKKLENATETERAEV
                     KRKYYEKLGFNEEAIALLGELAPPWTWTCVSNCTGSKNNQISEEARNCLRNELITMRG
                     RVTEHQIDDLLNGKRLKIWGNSQKTMFVSYSRGRLIEHIVDNYESFLN"
     gene            4783..5115
                     /locus_tag="PXD71_001087"
     CDS             4783..5115
                     /locus_tag="PXD71_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291222.1"
                     /translation="MVKFIRLNLINYFIEKDEYGEPVRCRAFRYDEIIQTKFIEGFRE
                     GEIPADDYGFEDWNGSVLILNEEGRKYFENYLFDVKKNYCRARDNYVYVLETLDEIEM
                     KLGLLEAK"
     gene            5115..5567
                     /gene="ssb"
                     /locus_tag="PXD71_001088"
     CDS             5115..5567
                     /gene="ssb"
                     /locus_tag="PXD71_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL6291223.1"
                     /translation="MNLVTLVGRLGQDPDIRTMQNGEKAAALSVATSEKWTDKQTGVK
                     KESTEWHRVVLYRRLAEIAELYVKKGHLVSIIGKIKTRKWTDSNGVERSITEIIAEQM
                     QMLSSGEKNTPNTPNKAENKPQPKQKNQDVMTADEQKDIPQFDDDIPF"
     gene            5578..5910
                     /locus_tag="PXD71_001089"
     CDS             5578..5910
                     /locus_tag="PXD71_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291224.1"
                     /translation="MREWEKQMPFSANLHIRPTGKFEVKNVNIEQIIGERGKNYGNFD
                     DVATMSQQLKAVLLDTNTQLKPHQKEAGEMICLKLARIFAGCNPDYEDNWRDIAGYAV
                     LGGKLNEK"
     gene            5900..6169
                     /locus_tag="PXD71_001090"
     CDS             5900..6169
                     /locus_tag="PXD71_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291225.1"
                     /translation="MKNKMGSKFIRVRVEHPQRKKEDIELVQDVVAYHIFNLFKFEES
                     GLRGFSQYDLEIDVKVKPVLEQKEMPVDVWIDDLGGKNGTITSGQ"
     gene            6144..6434
                     /locus_tag="PXD71_001091"
     CDS             6144..6434
                     /locus_tag="PXD71_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291226.1"
                     /translation="MEQLQAVSNEQVFAKLCEVERLLKTKGVNEHSRELWDLGDVAAY
                     FGYTKEHTSRSVVSSPHFPKPIALDGLRGKGRGAKKWVSGEVVKFCLMWKVK"
     gene            complement(6704..6886)
                     /locus_tag="PXD71_001092"
     CDS             complement(6704..6886)
                     /locus_tag="PXD71_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDL6291227.1"
                     /translation="MAMSRNEIQAKSEAKRGIKQKSFKLPLEVIAEIEVLSQKLNIPQ
                     NQLIIQAIQQFKQNNP"
     gene            complement(7164..8162)
                     /locus_tag="PXD71_001093"
     CDS             complement(7164..8162)
                     /locus_tag="PXD71_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL6291228.1"
                     /translation="MATIIKNGNKYRAQVRKKGIYKTATFSTKTEANRWAYALEAQID
                     AGEYNTTPNILFAELIDKYLKEVTPTKRGKREERYRLLRIAGSNLGNIPLPALTKEHL
                     RQWQNQRLQEVQPVSVSRERATLSALFTKAMEWDYLKENPLKTLEKIKTPPARTRRYS
                     DEEIDKLIFVSGYSPNQPPLLMQQRAGAALLFAIETAMRAGEIAGLKWEHINLQARTA
                     FLPQTKNGYSRTVPLSTKAIAILNQLAQLEPNKQNSVFQLTSRSLDSNFRKLKQKANL
                     HNADLHFHDTRREALTRLSKYLTVMELAKVSGHRDLSILQNTYYNPDISELVSKLQ"
     gene            complement(8390..8466)
                     /locus_tag="PXD71_001094"
     tRNA            complement(8390..8466)
                     /locus_tag="PXD71_001094"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8430..8432),aa:Val,seq:gac)
     gene            complement(8530..9330)
                     /gene="apaH"
                     /locus_tag="PXD71_001095"
     CDS             complement(8530..9330)
                     /gene="apaH"
                     /locus_tag="PXD71_001095"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDL6291229.1"
                     /translation="MATYIVGDLHGCFNEFQLLLEKVNYNPKQDELFLTGDLVARGED
                     SLACLRFVKDSANNAKTVLGNHDLHLLGIKRVKPNDKIDAIFEAKDRLDLQNWLRNQP
                     LVIHHPKHQFLLTHAGISPEWDLATTLACAKEAEKVLQSDNYADYIAQMYENKPERWS
                     EDLQGVERWRYIINVFTRMRFCYTDKRLDFDCKLPLEQAPQELLAWFDLDNPLFTQKN
                     IIFGHWASLMGKCTRPNIYALDTGCAWGSHLTMLRWEDKQIFTQKRLK"
     gene            complement(9422..11398)
                     /locus_tag="PXD71_001096"
     CDS             complement(9422..11398)
                     /locus_tag="PXD71_001096"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDL6291230.1"
                     /translation="MFQNNPLLAQLKQQIEASKEYVAGVVKASDKAFGFLECDKKSYF
                     IPPAEMKKVMHGDSVKAVVKRDGDKEQVEIDSLLEPMLDRFIAQVRLNKEGKLQLAVD
                     HPSIKQTIPANTHKKATEKLENGDWVVAQLKTHPLRDDRFFFAQVTQFICKESDNFAP
                     WWVTLARHEQPREPVANEKNYELHDELAREDLSHLYFTTIDSPSTQDMDDALFIEPIK
                     QNGKRTGWRLVVAIADPTAYIPENSHIEKAARQRCFTNYLPGFNIPMLPRELSDDLCS
                     LVPNEKRPALVGFIETDLNGNLIKETTFTSAWVESKDRLAYDDVSDYLEGVENAWQPQ
                     SEETKQQIEWLHQFTQARIQWRAENALLFKEQGDYTFELNEDGSVKDIHVEYRRIANQ
                     MIEESMILANICCAKFLSQHAKTGVFNTHSGFDPKNYELVKSFLLQTLASDENRDELA
                     ERYSAERLATLEGYCQMQRDIQDYPEKFLELRLRRYLTFAEFKAEVAPHFGLGITDYA
                     TWTSPIRKYGDMVNHRLIKQVLLGKQSQAVEESVLSRLQEARRQNRLVERDIADWLYA
                     RYLAPMVEQAVEFECEIADVSRGGLRAKVIKNGAQIFVPFSSLHDNKEEMEYRQEELA
                     LYIKGEKAYQIGQAVKVKLTEVRLETRSIVGNII"
     gene            11558..11836
                     /locus_tag="PXD71_001097"
     CDS             11558..11836
                     /locus_tag="PXD71_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL6291231.1"
                     /translation="MKKLLAIATVFSLAACSTQTLELSPAKEAQYDRVQHFFVSGLAQ
                     KQEIDAAKICNGADKVGKVETETTFFNGLLGTITYGIYSPRQIRVYCN"
     gene            11861..12157
                     /locus_tag="PXD71_001098"
     CDS             11861..12157
                     /locus_tag="PXD71_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL6291232.1"
                     /translation="MKKLLAMSVLTALLTTACTTQTAHIGGKVTDNIKPTKSVTQSFF
                     IGGIGQEETLNVVEVCGSKDKVQQVETVLSGGDILLGLVTIGIYTPRTANVYCK"
     gene            complement(12210..13001)
                     /locus_tag="PXD71_001099"
     CDS             complement(12210..13001)
                     /locus_tag="PXD71_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6291233.1"
                     /translation="MGILTGKRILVTGLASNRSIAYGIANAMKQQGAELAFTYLNDKL
                     KPRVEEFAKEFGSDIVLPLDVATDESITECFTELSKHWEKFDGFVHAIAFAPGDQLDG
                     DYVNAATREGYRIAHDISAYSFVAMAQAARPFLNENAALLTLSYLGAERAIPNYNVMC
                     LAKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRR
                     TVTIDDVGNSAAFLCSDLASGVTGEVLHVDAGFSVMAMGELGDEE"
     gene            complement(13145..13918)
                     /locus_tag="PXD71_001100"
     CDS             complement(13145..13918)
                     /locus_tag="PXD71_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HDL6291234.1"
                     /translation="MKIVSEPKTSGRHTCTIHCQNCSISQLCLPFTLNETELTQLDNI
                     IERKKPIQKSQVIFKLGDELRSLYAIRSGTLKAYTLSESGEEQITGFHLPGDLIGFDA
                     ITNMQHEGYAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLL
                     SKMNAEEKLAAFLYNLSQRYSARGFSAKEFRLTMTRGDIGNYLGLTIETISRLLGRFQ
                     KSGMISVQGKYIIINRMEELAELAGAIKPQQNIIAQTTA"
     gene            14149..14574
                     /locus_tag="PXD71_001101"
     CDS             14149..14574
                     /locus_tag="PXD71_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL6291235.1"
                     /translation="MYKHILVAVDLSEESLVLVRKGAALAEKCGAKLSLIHVDVNFSD
                     LYTGLIDINMSSVQYSVVEETNTALAELASKVNYPVSECLNGTGDFSQVLEEAVEKHN
                     VDLLITGHHQDFWSKFMSSTRRVMNNITVDMLVVPLADE"
BASE COUNT         4422 a         2596 c         3276 g         4347 t
ORIGIN      
        1 tatgactaat aagaaaattc agaaagctat tgaacttgta ggaacacaaa caaaattagc
       61 tgaagcttgt ggagttagtc agcctacggt cttgaaatgg ctaaacggag gcggaatttc
      121 cgctgaatat ctactaaaaa tcgaagctgc cacaaacggg caggtaacaa ttcgtgaaat
      181 ttgtgaggag ttgggaaatg caacagcaaa ataaaagccc tgaacaggca gggctagcga
      241 gtgggaaatt tactgtaaga cgtaatcaag acggtaaatg gcgtttagat ttgaccttgt
      301 tggattttgg tattagttcg ccagaaacga ctcaagctcg ttgcaaattg cttcaagagc
      361 ttcagcttgc tcttcttcaa ggtcaaggtc atttaaatac tgtcggcgtg aacgttcgaa
      421 aagccggtcc agtcgtttgc gttggtagtc tgttaagcga ccttttaaat catcaagaat
      481 aatgtttttg gcgtttagtt gatggcgtaa gttttcaact tcaacagtca gttcaataat
      541 ttgatcttca gtattcataa aatttccttt tggagggtta aacaatatct aaaacataac
      601 caaaattcga ctgcttgtct atggggatag ataagccgaa atcaacgtaa agaatgttaa
      661 tagagccttg gggaaggcgt aggggaaata atgaatttag accacacctg tacaaattgc
      721 aaaagtaaaa atttacgggt tcgtacatct gaaaaaatcg gtttgctgac tattcaggct
      781 gttacttatt gtaataactg cggcacagag caacttgtgc aaagtcaaat tatccgtgtt
      841 cgcacaccga tttataaaga gcgaccggaa gcaatgcgta ttaataaatg cttgatggaa
      901 gccgatttga acacgccgga tttgttcgat ggcgttgtgg cagaccaacc taagcaagac
      961 taatcccaat ttttaaacaa tcgtcaaaat aagccttatt tgcatagggc gagatttttg
     1021 caccctaaat tcaggaattt gaacaatgag taagcaatta tattaccgac acaaatcacg
     1081 ttggaacaga cttaaccgcc ttaagaagat gaataaacgt cgccttaatc tgtttttgca
     1141 agaaaagcgg gtggcgaatt tagaaagccg gttagctgat gaagtagagc tggcaaagct
     1201 gaatagtgag cagattggcg atttactgca agcgttggag ttaaaagtga atcggttaat
     1261 tcgccaaaat gaaaagctga aagcccgtgt gattaagctg gaaaaagtca aacagaccca
     1321 aaagcaaggt ttgtttgcaa tgtttaaaaa ggtgttgggt agcaagggta atccttagta
     1381 agaggtagat atggatttcg tggcaacgta tcaaaagata cgcattaaca ctaatcacaa
     1441 gcaatttact tgcaatcact taggcgagat ttgggggaag gaaaacgacg attttaaacg
     1501 tttcttacta cgagtcgcat tcttgcagga taaccccgag ccgaaacctt ggggagcgta
     1561 ttccccacaa gaaaaacgcc ttttacatag ttttagcttg tggttagagc agatagcaga
     1621 ttttcagcga gtaatgaaaa gagaatcaaa actgcgtgaa aaacatatta acgcaccgga
     1681 ttagaaggag taaatatgag ccgtaaccgt gaattgtgtg agttttttca caaactgtgg
     1741 tgcaaaaccg gtaatcgcta tgcttatcgc cggtatcaag tgttttttca gcgttatcaa
     1801 aacgaatgtt tagtgaatga gtagagggta gaaaaatgtc attaattact gtgaacgatt
     1861 acaaaatcaa gaaagatatt ttagatgcag acggcaagcc gtgttatcag ttgtatcaag
     1921 gcgaagagct attgcgtgat accaccaatt taagcgaaat catcgcttat atgtttattc
     1981 gtgccgaaga taaaccgttt gatgagttgt ataaccatat catcaaccaa gaagcaggag
     2041 ctaaaacaga tattaaattt acggtaacgg tgagatgagc cagattacta aatccgctta
     2101 cacacatatt cacccaaact ggcaagcccc gagaggtaac acttaccgga ttgagcgata
     2161 tgagctaaaa cggcggtatg ccttattttg taatggtgag agggtagttt tatataaaga
     2221 cccaacacac cccaataaga acgtagagag ccatatagaa agtgatgttt tgttgttatg
     2281 gctactgaaa aatgaattag ggtggagcgg taatattaaa gagccgttaa gccctcaaga
     2341 tgttgagaat atccgcttgt tagagcaagc attttgttta acttataacg gaactttcat
     2401 taaatgaatt atcagaatga gattctatct gctacagagg ctttcgcaat aggtgtttca
     2461 ctcccacctg ttgccggtgc ttctgcttgt gcattagagc ttcattctga tcgtttatat
     2521 caagcgttca aacagcctta cacttcaatg gtccagcaag aattattctc gaaagaccct
     2581 attcacgaac cgttacgcca atcttatttt gagcgtttgc ctcgtgcctt agcagagcat
     2641 ttcggcagac agtataagca aaagctacat taccaaagcc aatacgacac agcagactgg
     2701 ttcaaagcgg aaatggcacg caaaatgcca cgcattgagg cagttattag ccagtattgc
     2761 gatgtattcg attttctgaa gcaatcccat caagatttag attttttagc tgaattagat
     2821 agtaatgttt atttcggtgc cggtgttgat acgcaacaag cggtacaaaa aattaaacaa
     2881 gatgccgaag tgaaaggtat tcaactgcct atgcaccttt gccgtttaga gaatagctct
     2941 cgctatttaa aagctcacgg cattcgcccg cttgcttacc aaaccgaaga gcaaatcaaa
     3001 cagcttgcct taggtattgc ttatcgtgtt cgccaaattc agcaagataa catcgagcag
     3061 aatatgcaca aagcgacaga tggcgacaca gcctatagtg tgttgcttga atgctatcgg
     3121 gcaatgatgg gagaggtaaa taagctcaag attgattcgc cttatcagaa aaaagcgaga
     3181 aagggtagat taactgaaga ggaaattacc acaggctttt taaaaatgac ctgtgaaaaa
     3241 tggtggacta gaaagttatc taaaattgcc gaacagatga aagagcattt agctattgcg
     3301 tgcggtatgg tgaatatgct ttcgccttac tgttcaaatg ctcgcttgaa agcgtttgag
     3361 gctcaacgca aggcgaatat tgattatttg aaatcaatga tcattgccaa tatcgccgag
     3421 ccggaagagc aactttcatt atttgaaacg tggttgaagt ctgcctctaa cccgaaaatt
     3481 aaacgccttg aattactcac tcgtatgaat ggatttgagc gttatgcaga taagcaaggg
     3541 cacgaggggt ggtttattac cttgactgct ccgtctaagt atcacgcaat gctttcaaga
     3601 accagtagcg tgaacccgaa atggaatgga gcaagccctg cggaaacaca ggtttacctt
     3661 gtgaatactt gggcgaaaat ccgtgccaaa ctcaaccgtg agggcgtaat ggcttacggt
     3721 tttcgagtgg ctgaacctca cgctgacgct accccacact ggcatttaat tctattcact
     3781 cgtccggaag atatggaaaa gcttcgccgc gtgttcttga gttatgcctt agaggtcgat
     3841 ggcacagaag ccggtgcgaa aaaataccgt tgcaaattca agcgtattga gaaagagaaa
     3901 ggctcggcaa cgggctattt agtgaaatat gtttctaaaa acattgacgg ttttggaatg
     3961 gacggcgagc tttcagacga ggcaaatatt caggcgaaag agaatgccgc tcgtgtagga
     4021 gcgtggtcta gcgtttggtg tattcgacag ttccagcagt taggtaatat tccgatcagc
     4081 ttatggcgtg agttacgccg tttggggagt gtagagcaag aagatgaaac tttagagaaa
     4141 ctccgtgtga ttgcagatag cggtgagtgg gacgttttca ccgaagaatt aggcggtgcg
     4201 ttggttaaac gtgccgattt agttgcacgt attacttata ccgaacgcaa aagcgaaacc
     4261 ggtgaggcgt tatatacgat gtatcaagag ccatcattaa aagtgagcgg cattattaac
     4321 attaaaaacg gtgtgcagat taatacacgc ccgaaagagt ggagcattca acttaaacct
     4381 aaaaactggg aggagcaaca attacagaaa aaattagaaa atgcaaccga aacggaacga
     4441 gcggaggtta aacgtaaata ctacgagaag ttagggttta acgaagaggc tatcgcttta
     4501 ttaggggagc ttgctccccc ttggacttgg acttgtgtca gtaactgtac ggggtcaaaa
     4561 aataatcaga ttagtgaaga ggctcgaaat tgcctaagaa atgaactgat tacaatgcga
     4621 ggacgggtta ctgaacatca aatagacgat ttactaaacg gcaagcggtt aaaaatttgg
     4681 ggaaattcgc aaaaaacaat gtttgtgagt tatagccgtg ggcgtttaat tgagcatatt
     4741 gttgataatt atgagagttt tttgaattag gatattaaga aaatggttaa atttattcgt
     4801 ttaaatttga ttaattattt cattgagaaa gatgaatatg gagaaccggt gcgttgcaga
     4861 gcgtttcgtt atgatgagat tattcaaact aagtttattg aaggatttcg agagggagag
     4921 ataccagctg atgattatgg atttgaagat tggaatggtt cggttctgat tttaaatgaa
     4981 gaaggacgta aatattttga gaactatctt tttgatgtaa agaagaatta ttgtagagca
     5041 agggataact atgtatatgt cttggaaact ttagacgaaa ttgaaatgaa gttaggatta
     5101 ttggaggcga aataatgaac ttagtcacat tagtcggacg tttggggcaa gaccctgata
     5161 ttagaacaat gcaaaatggt gagaaagctg cggctttatc ggtagcgacc tctgaaaagt
     5221 ggacagataa gcaaacaggt gtgaagaaag aaagcaccga atggcatagg gtggtgcttt
     5281 atcgccgatt agctgaaatc gccgaattgt atgtaaaaaa agggcatttg gtatcaatta
     5341 tcgggaaaat taaaacccga aaatggacgg atagcaacgg tgttgagcgg agtattacag
     5401 agattattgc cgagcagatg caaatgctca gtagtggtga gaaaaatacg ccaaatacgc
     5461 caaataaggc agaaaataaa ccgcaaccaa agcagaaaaa tcaagacgtg atgactgcgg
     5521 acgagcagaa agatattccg cagtttgatg atgatattcc gttttagggg gtgaataatg
     5581 agagaatggg aaaaacaaat gccatttagt gcaaatttac atataagacc tacaggaaaa
     5641 tttgaagtga aaaatgtaaa catagaacaa ataatcggag aacgtggcaa aaattacggc
     5701 aactttgatg atgttgccac aatgagccag cagttaaaag cagtgttgct ggataccaat
     5761 actcaactta aaccacatca aaaagaggca ggcgagatga tctgcttgaa actggctcgc
     5821 atttttgcag gttgtaatcc ggactatgaa gataactggc gagatattgc gggttatgcg
     5881 gtgctgggag ggaaattaaa tgaaaaataa aatggggagt aaatttatac gagtcagagt
     5941 tgagcatcca cagcggaaaa aagaagatat tgagcttgtg caggatgtgg ttgcttatca
     6001 tatttttaat ctttttaaat ttgaggaaag tgggttacgg gggtttagtc agtatgattt
     6061 ggaaattgat gtgaaagtta agccagtgtt agaacaaaaa gaaatgcctg tagatgtttg
     6121 gattgatgat ttaggtggta aaaatggaac aattacaagc ggtcagtaat gagcaagttt
     6181 ttgcaaaact ttgtgaggtt gagcggttat taaaaaccaa aggggtgaat gagcatagcc
     6241 gtgaattgtg ggatttgggc gatgtagcag catatttcgg ctacactaaa gaacacacca
     6301 gccgaagtgt tgtatctagt ccacattttc cgaaaccgat tgcccttgac ggcttacgag
     6361 gcaagggcag gggggcgaag aagtgggtat ccggtgaagt ggtgaaattt tgtttgatgt
     6421 ggaaggtgaa gtgatgataa aaaagataat tttagatgct tgttgcggta gccgaatgtt
     6481 tcactttaat aagcacaacc cctcggtatt gtttgctgac aatcgggaat ccctgtatcg
     6541 aaaattttaa atgttatcga ataccaacct atctatgggc ataaaagcgg taaacataac
     6601 aaaacgcatt ggctggcatt tgtaaagatg gataataacg attatttgta aagtatttca
     6661 aaatacaaaa gccgataaat ttcattattt atcggctttt gttttatggg ttgttttgct
     6721 tgaattgttg aatagcttga ataatcagct ggttttgtgg aatgttgagt ttttggctaa
     6781 gtacttcgat ttctgcgatc acctcaagcg gtagtttaaa ggatttttgc tttattcctc
     6841 gttttgcctc gcttttagct tggatttcat ttcttgacat tgccataatt tatccctatt
     6901 ataaattttg ttgaagagct gggagctttc acccccagcg agttgcttca gattagtaag
     6961 ctggggagct aaggactaat aagaagacaa ctaggataat gtatttaaac attgtgctat
     7021 ccttcttcat ttcgtaaggt ttaagcctta cgctcacttt caagctgtct cttgaaagtg
     7081 aggttattat aatgtagggt atcctacatt gcaagtaata aatacaaaaa agccgataaa
     7141 ttttcgttta tcggcttttg tttttattgc agtttgctca caagttctga aatatccggg
     7201 ttatagtagg tattttgcaa aatacttaaa tctcgatgcc ccgatacttt ggcaagctcc
     7261 attaccgtca gatatttaga caaacgtgtg agtgcctctc gccgtgtgtc gtgaaaatgc
     7321 agatcggcat tgtgtagatt ggctttttgt ttcagcttgc gaaaattcga atcaaggctg
     7381 cgactggtga gctggaaaac actgttttgc ttgttcggtt caagttgtgc caactgattt
     7441 aaaattgcta ttgctttggt tgagagtggc acggtgcgtg aataaccgtt tttggtttgt
     7501 ggcaaaaatg cggtgcgtgc ctgcaggttt atatgttccc attttaaacc agcaatttca
     7561 cccgctcgca ttgcagtctc aatcgcaaat aatagtgctg ctccggctct ttgttgcatt
     7621 aatagcggtg gctggttagg gctgtagccg gagacaaaaa tcagtttgtc tatctcctca
     7681 tcgctatatc ggcgagttct tgctggtggc gttttgattt tttccagcgt ttttagagga
     7741 ttttctttga gataatccca ttccattgct tttgtaaaga gtgccgataa agtggctctt
     7801 tctcttgaga cggaaaccgg ctgcacttct tgcaagcgtt ggttttgcca ttggcgtaaa
     7861 tgctctttgg tgagtgccgg taatggtata tttcccaagt tgctgccggc aattcttagc
     7921 aggcgatagc gttcttccct tttgccacgt ttggtcggag tgacctcttt gaggtatttg
     7981 tcgattaatt cggcaaaaag aatgttcggg gtggtgttat attctccggc atcaatttga
     8041 gcctctaaag cgtaagccca gcggtttgcc tctgttttgg tgctgaaagt ggcagtttta
     8101 taaatgcctt ttttgcggac ttgtgctcgg tatttgttgc cgttcttaat aatagtagcc
     8161 atttttttat tcctttgtgt atgtgtgcag tttaagtgtg caaatgtgtg caaaggaatg
     8221 ttaatagaga tcataaacga tcaataaata tcaagttttc atatcaaaaa tgatgttttt
     8281 attgatgtta tgctacactt ggggaagtgt tttaaatcaa taagctattg atttataagg
     8341 ggtatttttg gggttaagcc tgaatgggga tattcaggct taaaaataat ggtgcgacta
     8401 gctggactcg aaccagtgac ccccaccatg tcaaggtggt gctctaacca actgagctat
     8461 agtcgcatta acggtgtgca ttctattgga aaaaggaact tagtgcaata tgcttttggt
     8521 cgaatgcttt tattttagac gcttttgggt aaaaatttgt ttatcttccc aacggagcat
     8581 ggttaaatgg cttccccaag cacagccggt atcaagtgca tagatgtttg gacgagtgca
     8641 tttgcccatt aggcttgccc agtgaccaaa gataatgttt ttctgtgtaa ataacggatt
     8701 atctaagtca aaccaagcca gcagttcttg aggggcttgt tcaagcggta atttacagtc
     8761 aaaatctaac cgcttgtcgg tgtagcaaaa ccgcatacgg gtaaacacat tgatgatata
     8821 acgccaacgt tcaacacctt gcaaatcttc actccagcgt tcgggcttat tctcatacat
     8881 ttgggcgatg tagtccgcat aattgtcact ttgtagcacc ttttctgcct ctttggcaca
     8941 agctagggta gttgctaaat cccactcagg gctgattcct gcgtgagtca gcaaaaattg
     9001 atgtttgggg tgatgaatta caagcggttg atttctgagc caattttgca aatcgagccg
     9061 atctttagcc tcaaaaatag catcaatctt atcatttggt tttacccgtt tgatgccaag
     9121 caagtgcaaa tcgtggttgc ctagcacggt tttggcattg tttgcagagt ctttcacaaa
     9181 acgtaaacag gctaaagagt cctcaccacg ggcgactaaa tcccctgtta aaaagagttc
     9241 gtcttgcttg gggttataat tgactttttc taataaaagc tgaaattcgt taaagcaacc
     9301 gtgtaggtcg cctacaatat aagttgccat tgtttctcct ttgttcccct aaagttataa
     9361 acaagcggtt atattttaga gaaaatttgc aaaatctcat caaaatataa ccgcttgaaa
     9421 tttaaatgat atttcctacg attgagcggg tttctaagcg tacttcggtg agtttcactt
     9481 tcaccgcttg cccgatctga taggcttttt cacctttaat ataaagtgca agttcttctt
     9541 ggcggtattc catttcctct ttgttgtcgt gcaagctgga gaatggcaca aaaatttgag
     9601 caccgttttt tatgactttc gcacgcaagc caccacggga aacatctgca atttcgcact
     9661 caaattcgac cgcttgttcc accattgggg caaggtaacg agcataaagc cagtcagcaa
     9721 tatcacgttc tactaaacgg ttttgacggc gagcttcttg taaacggctt aaaactgatt
     9781 cttccaccgc ttggctttgt ttgccaagta acacttgctt gattaaacgg tggtttacca
     9841 tatcgccata tttacggatg ggtgatgtcc aagtggcgta atcggtaatg cctagtccga
     9901 aatgcggggc aacttcggct ttaaattcag cgaaggttaa ataacgtcgt aaacgcaact
     9961 ctaagaattt ctctgggtag tcttgaatat cacgttgcat ttggcaatag ccttctaagg
    10021 ttgctaaacg ctcggcggag tagcgttcgg caagctcatc acgattttca tcgctggcta
    10081 aagtttggag taaaaatgat tttaccaatt cataattttt cggatcaaaa cctgaatggg
    10141 tattaaatac accggttttg gcatgttggc ttaagaattt tgcacaacaa atattggcaa
    10201 gaatcattga ttcttcgatc atttgattcg caatacggcg atattcaacg tgaatatctt
    10261 ttaccgagcc atcttcattt aactcaaagg tataatcgcc ttgttctttg aatagcaacg
    10321 cattctcggc acgccattga atgcgggctt gagtaaattg atgtagccac tcaatttgtt
    10381 gttttgtttc ttcagactga ggttgccacg cattttccac gccttcgaga taatcggaaa
    10441 catcgtcgta agccaagcga tcttttgatt cgacccaagc agaggtaaag gttgtctctt
    10501 taatgagatt gccgtttaaa tcggtttcaa taaagccaac gagggcaggg cgtttttcat
    10561 tcgggacaag cgaacagaga tcgtctgaga gttctcgagg taacattgga atgttaaagc
    10621 ccggcaaata gttggtgaaa caacgctggc gagcggcttt ttcaatatgg gaattttctg
    10681 ggatataagc ggttgggtca gcaatcgcta ccactaaacg ccagccggtt cgcttgccat
    10741 tttgcttaat tggttcgatg aacagagcat cgtccatatc ttgcgtgctt gggctgtcaa
    10801 tagtggtaaa gtaaagatga gataaatctt cacgagctaa ttcatcgtgt aattcatagt
    10861 ttttttcatt tgcgaccggt tcacgaggct gttcgtggcg ggcaagtgtg acccaccaag
    10921 gggcaaaatt atcagattct ttacagataa attgggtaac ttgagcaaag aaaaaacggt
    10981 cgtcacgcaa tgggtgcgtt tttaattgtg caaccaccca gtcgccattt tctaattttt
    11041 cagtggcttt tttatgggta tttgccggga tagtttgctt aatgcttggg tgatcaaccg
    11101 caagttgcaa tttgccctct ttattcaagc gaacttgggc aataaatcta tccagcattg
    11161 gctcaagtag tgaatcaatt tctacctgtt ctttgtcgcc atcacgtttg actacggctt
    11221 tcactgaatc accgtgcatt acttttttca tttccgcagg tggaataaaa tagctttttt
    11281 tatcgcattc taaaaaaccg aatgctttgt ctgaggcttt gacaacgcct gcgacatatt
    11341 ctttgcttgc ttcaatttgt tgtttgagtt gggctaaaag gggattgttt tgaaacataa
    11401 tttctcattt taaaaataaa aaaaggtgct atcgttgcac cgaaaaaaac aatggcatta
    11461 taacggattt gaaccaagtt cagcaaataa atgctgttgc tataacttat aaaggttggt
    11521 agaatataat ccatttaaaa ataatggaga cttttcaatg aaaaaattac ttgcaattgc
    11581 aacagtattt tctttagcag cctgttctac tcaaacactt gaactatctc ctgctaagga
    11641 agctcaatat gatcgtgttc aacacttctt tgtttcaggg cttgctcaaa aacaagagat
    11701 agatgcagca aaaatttgta atggtgcaga taaggtaggt aaggtagaaa ctgaaactac
    11761 tttctttaat ggcttgttag gtacaattac ttatggtatc tattcacctc gccaaatccg
    11821 tgtatattgt aactaatcct agtaatttaa ggagttgaaa atgaaaaaat tgcttgctat
    11881 gtctgtatta acagccctgc tgactacagc atgtacaaca caaactgccc atattggcgg
    11941 caaagttact gataatatta aaccaactaa gagtgtaact cagtcgttct ttataggtgg
    12001 gattgggcaa gaagaaacat tgaatgttgt agaggtttgt ggttccaaag ataaagtgca
    12061 gcaggttgaa acagtacttt caggtgggga cattctatta ggtcttgtga ctatcggtat
    12121 ttatacacct cgtactgcaa atgtatattg taagtaatat tttattaaaa aaataacgga
    12181 ctcaattgag tccgttattt ttataggaat tactcttcgt cgcctaattc acccattgcc
    12241 attacgctga agccggcatc aacgtgtaac acttcacctg ttacaccaga ggctaaatct
    12301 gagcataaga aagctgcaga gttacctaca tcatcaatag ttacggtgcg gcgtaaagca
    12361 gcggttttct cgaaagcaga aagcattttc ttgaagtttt taatgcctga agcagctaat
    12421 gtacggatcg ggccggctga aatcgcattt acacgaatac cttctttacc taaatcggct
    12481 gccattacac gggttgcagc ttctaaagag gctttggcta agcacattac attgtagttg
    12541 gggattgcac gctctgcacc taagtaactt aaggttaaaa gggcggcatt ttcatttaag
    12601 aaggggcgag cagcttgagc cattgcaaca aagctatagg cactgatgtc gtgtgcaata
    12661 cgataacctt cacgggttgc ggcatttaca taatcgccgt ctaattgatc gcccggtgca
    12721 aatgcgatag cgtgaacaaa accgtcaaat ttttcccaat gtttgcttaa ttcagtaaag
    12781 cactcagtaa tgctttcatc ggttgctaca tctaacggaa ggacaatgtc tgagccaaat
    12841 tctttggcaa attcttcaac tcgtggtttt aatttatcgt ttaagtaggt gaatgcaagt
    12901 tctgcacctt gttgcttcat tgcgtttgca ataccgtaag cgatcgaacg gttgcttgct
    12961 aaacctgtca ctaaaatacg tttaccagtt aagataccca taaaaattcc tcttttatat
    13021 tgattggggc aaaattgtgt cgattataac ggatatagga aaaacgggca aatggagtta
    13081 gcggctcatc ggatcagaaa tccctttttg aacgaggttt caaaaaggga tttggtaaga
    13141 taaattaagc ggtagtttga gcaataatat tttgctgcgg tttgattgcc ccggctaatt
    13201 cggctaattc ttccatacgg ttaataataa tatattttcc ttgtactgaa atcataccgc
    13261 ttttttggaa acgccctaat aaacggctga tagtttcaat ggttaaacca agataattgc
    13321 cgatatctcc acgggtcata gttagacgga actcttttgc agagaaacca cgggctgagt
    13381 aacgttggga aagattatac aaaaatgcag ctagtttttc ttccgcattc atttttgaga
    13441 gcaatagaat catttcttga tcgcttttaa tttcattact catcaaacgc ataatttgat
    13501 ggcgaatttt tggcattttg ccggcaagat catctaaaat atcgaatgga atctcacaaa
    13561 tcattgaggt ttcgagtgct tgagcatagc cttcgtgctg catattggta attgcatcaa
    13621 agccaattag atcgccaggt aaatggaaac cggtaatttg ctcttcacca ctttcactta
    13681 aggtataggc ttttaatgtt ccagaacgga tagcataaag cgaacgcagt tcatcaccta
    13741 acttaaaaat aacttgcgat ttttgaatcg gttttttacg ctcaataata ttatctagtt
    13801 gagttaattc cgtttcatta agagtaaaag gtaagcatag ttggctaata ctgcaatttt
    13861 gacaatgaat tgtgcaagta tgacgtccgc ttgttttagg ttctgataca attttcatcc
    13921 attcaacctt aaattagaaa agtattccta aatttgattt agaacaaagt ttaccattaa
    13981 atttacatga agcaaagtat accaaggaaa aataaaaata cttacaaaga ggtatgaaaa
    14041 atataatcaa atttttattt ttgagatcta cttcaagttt ttatcaaaaa gagtgtgtta
    14101 gtatgtttat acacaaagta aacaactctc acaagcaagg agcatattat gtataaacat
    14161 atcttagttg cagtggatct ttctgaagaa agtcttgttt tagtgcgtaa gggggctgcg
    14221 ttagcagaaa aatgcggagc taaactttcc ctcattcacg ttgatgttaa tttttcggat
    14281 ctctatacag gattaattga tattaatatg tcttcggtac aatacagtgt tgttgaagaa
    14341 acaaatacgg cactggctga acttgcctca aaagtaaatt atcctgtatc tgaatgttta
    14401 aacggaacgg gagattttag tcaagttttg gaagaggccg tagaaaaaca caatgtcgat
    14461 ttactcatca ctggccatca tcaggacttc tggagtaaat ttatgtcttc aactcgtcga
    14521 gtcatgaata atatcactgt tgatatgctg gttgtgccat tagcagatga atagcaagaa
    14581 agtgaatgaa aaataaccgc ttgagacagt ttatctgtct tacaagcggt tatttttttg
    14641 g
//
LOCUS       DANLXS010000050        14626 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.050, whole genome shotgun sequence.
ACCESSION   DANLXS010000050 DANLXS010000000
VERSION     DANLXS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14626
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.050"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            98..1990
                     /gene="mnmG"
                     /locus_tag="PXD71_001102"
     CDS             98..1990
                     /gene="mnmG"
                     /locus_tag="PXD71_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL6291236.1"
                     /translation="MIYNQIYDVIVVGGGHAGTEAALAPARMGLKTLILTHNVDTLGQ
                     MSCNPAIGGIGKGHLVKEIDAMGGLMAKATDVAGIQFRTLNNSKGPAVRATRAQADRV
                     LYRNAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARAVVLTAGTFLA
                     GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQ
                     HGDEVLPVFSFMGDVSQHPRQIPCYITHTNDQTHDLIRNSLDRSPMYTGIIEGVGPRY
                     CPSIEDKVMRFADRNSHQIYLEPEGLNTIEVYPNGISTSLPFDVQMGIVNSMKGLENT
                     RIIKPGYAIEYDYFDPRDLKPTLETKAIDGLFFAGQINGTTGYEEAGAQGLLAGINAA
                     LQVQGKEAWYPTRDQAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTP
                     KAHELGLIDEARWVRFNQKMEAIEQERARLKSTWAHLQMPNLDELNALLANPLAREAS
                     GEDLIRRPEMSYEKLTQIALFAPAIEDKQVAEQVEIAIKYQGYIEHQYNEIERHKRHE
                     NTLIPVEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGM
                     LKRGEL"
     gene            2085..2309
                     /locus_tag="PXD71_001103"
     CDS             2085..2309
                     /locus_tag="PXD71_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291237.1"
                     /translation="MIYLRKLGFSPFDHQMFAQPSWYFCNALVHTNYENLRQGVFVTK
                     AYLVMFLENLLFEKSHSLSNRALRINENND"
     gene            2322..2936
                     /gene="rsmG"
                     /locus_tag="PXD71_001104"
     CDS             2322..2936
                     /gene="rsmG"
                     /locus_tag="PXD71_001104"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL6291238.1"
                     /translation="MLAKLNRLLAQAEINLTDQQKEQLVGFVRLLDKWNKAYNLTSVR
                     NPDEMLVKHILDSLVVSRHLQGETFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRI
                     TFIKNALRELGIKNVTPVQSRVEEYTESQFDGVLSRAFASLNDMIDWCYHLPTENGKF
                     YALKGVYDEQEIIEIKQPIQLVEVIKLEVPELVGERHLVVLKKP"
     gene            3204..4652
                     /locus_tag="PXD71_001105"
     CDS             3204..4652
                     /locus_tag="PXD71_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6291239.1"
                     /translation="MNRVTNKADEFAKLSTFERIAYGSGDLAQNLVFGTVGGFLLTYL
                     TTVNGISAVAGATIFLIVKWGNVFWDPFVGAFIDKSKPSAQGKYRPYLLKFGIPLVVL
                     ASLLFLPLESVRGSVIYAFLSYFATAMVYSFVNIPYGSLSASLTRDTDEIAKLTTTRM
                     TMANIANLFVYTLFPLFVQLLAPHAELKNTGFFGIELYLGDYMSAAAGPAWFKVYAVY
                     MVIGFVALMLSYFGTKERIIPTAEETDKVKYSDLVNELKRNRPLQILGLFFLIGFTFM
                     FFGNTVWPFYLKYNIGHSEWIASVGLLGSIPGIFLVFLWPKLRRTIGKKNFFYLFLGI
                     FVLGQLCLWVWQFDSFKYSPTLGYIGRFLQQWGLTSATGFMWALVPEVVTFGEFKSKK
                     RVAGIINALMGLFFKIGLALGGIIPGYINAVYGFNGSLLAQTEEALFGINLSMIWAPI
                     ILAIIAGFVMSRYPLSDTDIDHINEKITAYSK"
     gene            4675..5721
                     /locus_tag="PXD71_001106"
     CDS             4675..5721
                     /locus_tag="PXD71_001106"
                     /inference="COORDINATES: protein motif:HMM:NF016497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL6291240.1"
                     /translation="MQIQNPVLPGFNPDPSIVRVGDTYYIATSTFEWFPGVRIHESKD
                     LVHWNLVKNVLDTTTLLDMRGNPSSGGIWAPDLSYAEGKFWLVYTDVKITDGAFKDMT
                     NYLTTAKEIQGPWSAPIKLNGIGFDASLFHDDDGRKYLVQQTWDHREYHHPFDGITLT
                     EFDVATMKLKPETQRTIFEGTEVKLVEGPHIYKINGEYYLFAAEGGTVFTHQEVVARS
                     KTLEAHSFQLAPNTPFITNFDTPNSYLQKQGHGSLVDTPSGEWYYASLTARPWNHATE
                     SATDPRGWSTLGREAAIQKVEFDQDGWPYIVGGHGGKTFVEAPKDAIFTEAPIDHSQH
                     DEFDSSTLDINWNT"
     gene            5724..6362
                     /locus_tag="PXD71_001107"
     CDS             5724..6362
                     /locus_tag="PXD71_001107"
                     /inference="COORDINATES: protein motif:HMM:NF036826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,4-xylosidase"
                     /protein_id="HDL6291241.1"
                     /translation="MPFSKEMGEVGNGVLKLIGRGSLANTHDLSLIARRWQAFYFDAE
                     TKVKFTPFSYQSMAGLTNYYNHSHWSWIFITKNDQGQQVIEVAENKGGLRNGQYTSYL
                     KDKAIVIPDGTEYVWFKTKVRKETYRYEYSFDGKQWHTIPVELDAAVLSDDYVLRSYG
                     GFFTGAFVGLAAVDYSSYESTAEFSYFDYKELGDKQLTDGSFSWEKSESCFG"
     gene            6381..7361
                     /locus_tag="PXD71_001108"
     CDS             6381..7361
                     /locus_tag="PXD71_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL6291242.1"
                     /translation="MKQRRFGKTNKMVSEIGLGTWQLGTKWGEPFNHQEAMAILETAY
                     EQGINFIDTADVYNNGQSEKAIGDILKKYPDFFYVVTKCGRALNPHTAEMYTPQAIEK
                     FVDGSLSHLGTEKLDMILLHCPPTLVYRNDEIFTKLEQLKAGGKLIDYGVSIETAEEG
                     LLAMEYDIAAMEVIFNMFRLKPLEQLFPTAQQKNVGIIARVLLASGLLTGRYNACTQF
                     GKDDHRTFNRNGEAFDKGETFSGVDYQLGLQAVEELKALFGTEDLIPYALRWVLMNEA
                     VSTVIPGASKVSQVITNAAVDNFPPLTENEMQKVEDIYNRLIRPSVHHLW"
     gene            complement(7423..8763)
                     /locus_tag="PXD71_001109"
     CDS             complement(7423..8763)
                     /locus_tag="PXD71_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6291243.1"
                     /translation="MFSFLKASPPAEQKVEASRVDAEYRKLRWQVFAGVFIGYAAYYL
                     IRKNFSLAMPYLIDEYGFTKADLGTVGVALSLAYGFSKFIMGNVSDRSNPKYFITIGL
                     LGSAIVSLVFGLVPGVLSSIPIMIILAALNGWFQGMGYPPGAKTMTNWFSTSERGVWW
                     SWWNVSHNLGGGLIGPLAILGLSIFGVWQSLFYLPALIAIVLAFVMFYLMRDTPESQG
                     LPPIDEYKGEKVVEKVESAHTLSSKDIFYKYILNNKFLWAIAIANVFVYFIRYGIIDW
                     APTYLKEVKHFSVDKQSWAYFLYEYAGIFGMLASGYLSDKVFKGHRAPPMLLFLAGVL
                     IAIVVYWKNPAGNPLIDNICLVAIGFLIYGPVMMIGLQAADLVPRVATGTATGLTGLF
                     GYLLGSASAGYVMGKLVDMFGWDGGFYALILSCFLAFAFIALTLFKKTSKQLEV"
     gene            complement(8953..10012)
                     /gene="glpQ"
                     /locus_tag="PXD71_001110"
                     /pseudo
     CDS             complement(8953..10012)
                     /gene="glpQ"
                     /locus_tag="PXD71_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(10082..11527)
                     /gene="glpT"
                     /locus_tag="PXD71_001111"
     CDS             complement(10082..11527)
                     /gene="glpT"
                     /locus_tag="PXD71_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDL6291244.1"
                     /translation="MFGPFKPAPHIAELPADKVDARYKFLRWQVFAGIFFGYAAYYFV
                     RANFDLAQKGLIEAGMYTKTDLGVIGTAAGLAYGLSKFVMAGMSDRSNPKVFLPTGLL
                     LSGLCMTLMGLMPWATSSILVMWVMIFLNGWFQGMGWPPCGRTMVHWWSKSERGTIVS
                     IWNTAHNLGGMVPGAMVLLASALYFSEYGVEATAKDVWQQALYFPGIAAMIAAIPIYL
                     VMKDTPQSCGLPPVEKWRNDYPDDYNEKTYEHDLSTKEIFVTYVLKNKLLWYIAIANV
                     FVYLIRYGVLKWSPVYLGEVKHFNIKGTAWAYTIYELAAIPGTLLCGWVSDKLFKGKR
                     GLTGFIFMILTTAAVVAFWLNPATPESEIAQYAGHAWYENPYQLMDFIWMTTIGFLIY
                     GPVMLIGLHALELAPKKAAGTAAGFTGLFGYLGGTVAASAVVGWAADKFGWDGGFYVM
                     IGGGVLAILLTFIVMVEEGKHKAKLEDHYGK"
     gene            11885..13480
                     /locus_tag="PXD71_001112"
     CDS             11885..13480
                     /locus_tag="PXD71_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDL6291245.1"
                     /translation="MQRHEQLAQVQQTQKWDFIIIGGGASGLGIAVDAASRGYKTLLL
                     ESHDFAKGTSSRSTKLVHGGVRYLANGDVALVREALRERGRLAKNAAHLFKNQSFIIP
                     NYKFKDCVMYRVGLGLYDMLSGNLSLGKTENISKTEAKRRLPTLNDTGLRTGTVYQDG
                     QFDDSRLAVNMAQTVVEHGGTVLNHAKVTSLHKTPQGKIDGVTFYDELNQQEHTVFGT
                     AVINATGVFMNDILSMDHGSTKKFVVPSQGIHLVLDKSFLPSNDALMIPKTSDGRVLF
                     AVPWHEVLVVGTTDTLIENPSYEPIPLEQEVNFILETAAQYLTKKPTRDDVKAVFAGQ
                     RPLAAPEKEGQSTKEVSRSHKVLTDATSGLITITGGKWTTYRQMAEDTVEEALKVHSE
                     LGNKPCVTVNLPIHGNIPAAQVNLQDHLYVYGSDIPQLRALMTETPEYAQKIHPRLDY
                     TVAEVIWAVRHEMAQTVEDVLARRVRLLFTDARAAIDSAAKVAAIMAKELGKDEMWQQ
                     QQVIQFLDVAKHYLLVDYQPQPI"
     gene            complement(13567..13866)
                     /locus_tag="PXD71_001113"
     CDS             complement(13567..13866)
                     /locus_tag="PXD71_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291246.1"
                     /translation="MENIKMEELKLTEKELLVLKGNALRMRFKAETNKTKNEIMQPFK
                     QVKQTGLALVNSPAVKTIALDFLVKRLLSMKGISFSVLGLTAFFLLQQKHKRDDS"
     gene            complement(13853..14248)
                     /locus_tag="PXD71_001114"
     CDS             complement(13853..14248)
                     /locus_tag="PXD71_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL6291247.1"
                     /translation="MLDNIKSGFRNSLITLLEIAQVRGEMARIEINEQKNQLISVLIF
                     ILLAFVFLLVSFVSLLFGLDNYLLPEQKVKVFFSIAIIMFIAVTICGMMILSALKKQR
                     HFMQSTLTELKLDIAAFKSALTLKQNGEH"
     gene            complement(14328..>14626)
                     /locus_tag="PXD71_001115"
     CDS             complement(14328..>14626)
                     /locus_tag="PXD71_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDL6291248.1"
                     /translation="DAKREELTNEIRDILKNAEELFNETSDSSTEEYKKLKDKLAKQF
                     DDVKDRFATLKDDTVDSARTVVKQTDTLVQDNPYKAITVAGVVGLLLGVLISRR"
BASE COUNT         4364 a         3001 c         3026 g         4235 t
ORIGIN      
        1 tcttaacctc tctaaaaatc tatttcccag atgatccctt tttacgctaa aatatgcgtt
       61 catttttcag cagaatttgt aagatagtag aagagatatg atttacaacc aaatttacga
      121 tgtgatcgtg gtcggcggtg gtcacgccgg cacagaagcg gccctagcac cggcacgaat
      181 gggcttaaaa accctgattt taacccacaa tgttgatacc ttagggcaaa tgtcgtgcaa
      241 cccggctatc ggagggatcg gcaaaggcca tctggttaaa gagatcgatg cgatgggcgg
      301 tttaatggca aaagcaacag acgttgccgg gatccaattc cgtaccctaa acaactcaaa
      361 agggcctgcg gtgcgtgcca cccgtgccca agctgaccgt gtgctttacc gtaatgcggt
      421 acgcactgcg ttagaaaatc aaccaaattt agatattttc cagcaagaag tagtggatat
      481 tttggtggaa aacaaccgtg cagtgggtgc agttaccaaa atgggcttaa cgtttaaagc
      541 ccgtgcggta gttttaaccg ctggtacgtt cttagcgggt aagatccaca ttgggttgga
      601 taactatgca ggtggtcgtg ccggcgatcc tgctgcaaca atgttggcag atcgtctgcg
      661 tgatctgaat ttgcgtgttg atcgccttaa aacaggtaca ccacctcgcc tcgatgcccg
      721 taccatcaac tttgacgtac ttgccaaaca gcacggcgat gaggtgttac ctgtcttctc
      781 ttttatgggc gatgtctcac agcacccacg ccaaattccg tgctacatca cccacactaa
      841 cgatcaaacc cacgatttaa ttcgcaacag tcttgatcgc agcccgatgt acaccggtat
      901 tatcgaaggg gttggtccac gttactgccc gtctattgaa gataaagtaa tgcgttttgc
      961 cgatcgtaat agccaccaga tctaccttga accggaaggc ttaaatacga ttgaggttta
     1021 cccgaacggg atctccacca gcttgccgtt tgatgtgcaa atggggatcg tcaactcaat
     1081 gaaaggcttg gaaaacaccc gcatcatcaa accgggctat gcgattgagt acgactactt
     1141 cgacccgcgt gatttaaaac caacccttga aaccaaagcg attgacggtt tattctttgc
     1201 cggacaaatt aacggcacga ccggttacga agaggcaggt gcgcaaggtt tgctggcagg
     1261 gattaacgca gcgttacagg ttcaaggtaa agaggcgtgg tatccaaccc gtgatcaagc
     1321 ctacaccggc gtgttggtgg acgacctctg tacattaggc accaaagagc cataccgtgt
     1381 gtttacctct cgtgccgaat accgtctgtt gctgcgtgaa gacaatgcgg acattcgttt
     1441 aacaccaaaa gcccacgaac taggattaat tgacgaggca cgttgggtgc gtttcaacca
     1501 aaaaatggaa gcgattgagc aagaaagagc tcgtttgaaa tccacttggg cacatttgca
     1561 aatgccaaat ctggacgaat tgaacgcgtt attggctaat cctttagccc gtgaagcaag
     1621 cggtgaagac ttaatccgcc gtcctgaaat gagttacgaa aagctgaccc aaatcgccct
     1681 atttgcccct gctattgaag acaagcaagt ggcagaacag gtggaaattg cgatcaaata
     1741 tcagggctat attgagcatc aatacaacga aatcgaacgc cataaacgcc acgaaaacac
     1801 cctgattccg gtagagtttg actacgacaa agtcgaaagc ctctccaacg aagtgcgagc
     1861 taaattgatg caacaccgcc ctgtctctat cgggcaggca agccgcattt ccggcatcac
     1921 accggcagcg atttccattc tgttggtgaa tttgaagaag caaggaatgt tgaaacgggg
     1981 tgagctgtaa atactacaag cggttggttt tgcagaaatt tttgcaaaat tgaccgcttg
     2041 tattctttgg agaaggaaat acccgaacta ccgccgtatt tttaatgata tatctgcgta
     2101 aactcggttt ttcgccgttc gatcaccaaa tgtttgccca accttcgtgg tatttctgca
     2161 atgcacttgt tcataccaat tatgaaaatc tgcgacaagg tgtatttgtg actaaagcgt
     2221 atttagtgat gtttcttgaa aatttattgt ttgaaaaaag tcattcgtta agtaatcggg
     2281 cattgcgtat taatgaaaat aatgattaag gaatttgcaa aatgttagct aaattaaacc
     2341 gcttgcttgc tcaagccgaa ataaatttga ccgatcaaca aaaagagcag ttagttggtt
     2401 ttgtgagatt attggataaa tggaataagg cgtataacct cacttccgtt cgcaaccctg
     2461 atgaaatgtt ggttaaacat attttggaca gccttgttgt tagtcgtcat ttacaaggcg
     2521 aaacgtttat tgatgtcggc acggggccgg ggctaccggg cattccgctg gcgattgcta
     2581 accctgataa acagttcgtg ctactagaca gcttaggcaa acgcatcact tttatcaaaa
     2641 atgcgttgcg tgagcttggt attaaaaacg ttaccccagt gcaaagtcga gtggaggaat
     2701 acactgaaag ccagtttgac ggcgtattaa gccgagcctt tgcctcgctt aatgatatga
     2761 tcgactggtg ttatcattta ccgactgaaa acggtaaatt ttatgcatta aaaggcgtgt
     2821 atgatgagca ggaaataatt gaaatcaaac aaccgattca gctagtggaa gtgattaagc
     2881 ttgaagtacc tgagttggta ggagaacggc atttagtagt gttgaaaaag ccctaataca
     2941 ttggaagttt aatgatacaa gcggtcgaat ttgtaaattt ttttgcaaat ctgcctgctt
     3001 gtgttaacta tttttcataa agcatacctg gttaataagg tgtgcttttt ttataaccta
     3061 ttatttcatc tgatttattt ctattttgtg atgtgcttca caaaaacaaa aaaacataat
     3121 gtagtaaaaa atattcgtaa ttgaagccct tatttcttta cctgaaaata agcgaactta
     3181 attatataac gaggaggttc gtaatgaaca gggtaacaaa taaggcagat gagtttgcta
     3241 aactcagtac gtttgaacgt attgcttatg gtagtggcga cttagcccaa aatttagttt
     3301 ttggtacagt tggtggtttc ttactcactt acttaactac tgtaaacggc atttctgccg
     3361 tagcaggagc aacgattttc ttaattgtaa aatggggaaa tgtgttttgg gatccgtttg
     3421 tcggtgcctt tattgacaaa agcaagccct cagcacaggg taaataccgc ccgtatttac
     3481 tgaaatttgg gattcctctt gtggttttag cttcactact atttcttcct cttgaatcag
     3541 tacgtggctc agtgatttat gctttccttt cttattttgc cactgcgatg gtttattctt
     3601 ttgttaatat tccttatggc tcgctttctg cttctcttac tcgagacaca gacgaaattg
     3661 ctaaattaac cacaacccgt atgacaatgg caaatatcgc taatttgttt gtttatacct
     3721 tattcccact ttttgtgcag ttacttgcac cacatgccga attaaaaaac accggctttt
     3781 tcggtattga gctctattta ggtgattata tgtctgctgc tgcaggccct gcttggttta
     3841 aggtttatgc ggtctatatg gtaattggat ttgtggcgtt aatgctctct tattttggta
     3901 ctaaagagcg gattattcca acagcggaag aaacagataa agtcaagtat tcagatttag
     3961 ttaatgaact caaacgcaat cgtccattac agattttagg gttattcttc ttaataggtt
     4021 ttacttttat gttcttcgga aatacggtgt ggccttttta cctaaaatat aatattgggc
     4081 attctgagtg gattgcctcc gtcggtttat taggctctat ccctgggatt ttccttgtat
     4141 tcttatggcc gaaactacgt cgcactatcg gtaaaaagaa ctttttctat ctatttttag
     4201 ggattttcgt tctagggcaa ctttgtctat gggtatggca atttgactcc ttcaaatact
     4261 ctccaacctt aggctatatc ggtcgtttct tacaacaatg gggattaact tctgctaccg
     4321 gttttatgtg ggcgttagtg cctgaggttg ttacctttgg agagtttaag tcgaaaaaac
     4381 gtgtagcagg tattattaat gcgttaatgg gcttgttctt taaaatcggc ttggcgttag
     4441 gcggtattat tccgggttat attaatgcgg tttatggttt taacggctcg cttttggctc
     4501 aaaccgaaga ggctttattt ggtatcaatc tttcaatgat ttgggctcca attattttag
     4561 cgattattgc aggatttgtg atgagtcgct atccgttaag cgatactgat attgatcata
     4621 tcaacgaaaa aattactgct tattccaaat aatttgagta taggagaata gattatgcaa
     4681 attcaaaatc cagttttacc cggtttcaat ccagatccat ctattgtgcg tgtgggtgat
     4741 acttactaca ttgcaacttc tacttttgaa tggtttccgg gagtgagaat tcacgaatcg
     4801 aaagatttag tgcattggaa tttagttaaa aatgtcttag atacaaccac gttgttagat
     4861 atgcgaggca atccctcttc aggtggtatc tgggctcctg atctttctta tgcggaaggt
     4921 aaattttggt tggtttatac cgatgtaaaa attacagacg gtgcattcaa agatatgact
     4981 aactatctaa ctactgccaa agaaattcaa ggtccttggt cagctccgat taaattgaat
     5041 ggtattgggt ttgatgcttc gttgtttcac gatgatgatg ggcgaaaata tttagtgcaa
     5101 cagacttggg atcaccgtga atatcatcac ccctttgacg gtattacgtt aacggaattt
     5161 gatgttgcca caatgaaact caaacctgaa acccagcgta cgatttttga aggtacagaa
     5221 gtgaaattgg tggaaggtcc acatatttat aaaattaatg gcgaatatta tctgtttgcg
     5281 gcggaaggcg gcacagtatt tacgcatcaa gaagtagtgg ctcgttctaa aacattggag
     5341 gctcactctt tccaactggc tccaaataca ccgtttatta ctaattttga tacacctaat
     5401 tcttacctac aaaaacaagg gcacggttca ttagttgata ccccaagtgg tgaatggtat
     5461 tacgcttccc tcacagcaag accttggaat catgcaacag aatccgcaac cgatcctcgt
     5521 ggttggtcaa cattagggcg tgaagccgcc attcaaaaag tagaatttga tcaagacggc
     5581 tggccttata ttgtgggtgg acacggtgga aaaacctttg tagaagcccc aaaagatgca
     5641 atttttaccg aagctcctat tgatcattct caacacgatg agtttgatag ctcaacgtta
     5701 gatattaact ggaacactta agagtgcctt ttagtaaaga gatgggcgaa gttggtaatg
     5761 gtgttttaaa attgattggg cgaggctctc ttgcaaatac acacgattta tcgctaatcg
     5821 cacgccgttg gcaagccttc tattttgatg cggaaaccaa agtgaaattt acaccgtttt
     5881 cttatcaatc tatggcaggt ttaactaact attacaatca ctcacactgg tcttggattt
     5941 tcatcaccaa aaatgatcaa ggacaacagg tgattgaagt tgcagaaaat aaaggtggac
     6001 tcagaaatgg gcaatatact tcttacctta aagataaagc aattgtcatt cctgatggaa
     6061 cggagtatgt ttggtttaag actaaagttc ggaaagaaac ttaccgttat gaatattcat
     6121 tcgatggcaa acaatggcac actattccgg ttgaattaga tgcggcagtg ctttcagatg
     6181 attatgtgtt acgcagttat ggcggtttct tcacaggagc ttttgtcggg ctggctgcag
     6241 tggattactc cagttatgaa tctacagcag aatttagcta ctttgactat aaagagttag
     6301 gcgataagca acttacagac ggctcattct cttgggaaaa atctgaaagt tgctttggct
     6361 aaaatgagga gaatatcaca atgaaacaac gcagatttgg taaaaccaac aaaatggtgt
     6421 cagaaattgg gttaggcact tggcagcttg ggacgaaatg gggtgaaccg ttcaatcatc
     6481 aagaggcgat ggcaatttta gaaaccgctt atgagcaagg catcaatttt attgataccg
     6541 ctgatgtgta taacaacggg caaagcgaga aagccattgg tgatattttg aaaaaatacc
     6601 ctgacttttt ctatgtcgtt accaaatgtg gacgtgcgtt aaacccgcat actgctgaaa
     6661 tgtacacgcc acaggcaatc gaaaaatttg ttgatggtag tttaagccat cttggcacgg
     6721 aaaagttgga tatgattttg ctccactgcc cgccaacgtt ggtttatcgc aatgatgaga
     6781 tttttacgaa attggaacag cttaaagccg gcggaaaatt aatagactac ggtgtgagta
     6841 tcgaaacggc agaggaaggc ttgttggcaa tggagtatga cattgcggca atggaagtta
     6901 tcttcaatat gttccgctta aaaccattgg agcaactctt cccaaccgct cagcaaaaaa
     6961 atgtgggtat tatcgcccgt gtactgttgg caagtggttt gcttacaggc agatataatg
     7021 cctgcaccca atttggcaaa gacgaccacc gcacatttaa ccgcaatggt gaagccttcg
     7081 ataaaggcga aaccttttcg ggtgtggatt atcaactcgg tttacaagcg gtggaagaat
     7141 tgaaagccct attcggcacg gaagatttaa ttccttacgc cctacgttgg gtattgatga
     7201 atgaagctgt gagtacagta atcccgggag caagtaaggt ttcacaagta attactaacg
     7261 cagcggtgga taatttccct ccactcactg aaaatgaaat gcaaaaagtg gaagatattt
     7321 acaaccgctt aattcgccca agtgtgcatc atttgtggta atttatccaa cgctataaaa
     7381 aatggaactc taacttgagt tccatttttg tcaattttag ccttaaacct ctaactgttt
     7441 agaggttttc ttaaacagag tcaatgcaat aaacgcaaag gctaagaaac aggaaaggat
     7501 cagggcatag aaaccaccgt cccagccgaa catatcaacc agtttaccca tcacataacc
     7561 tgcacttgca gagcctaaca agtagccgaa taaaccggtt aaacctgttg ctgtaccggt
     7621 ggcaacacga ggcactaaat ccgctgcttg taaaccgatc atcatcaccg gcccgtagat
     7681 taagaaacca atcgccacta agcagatgtt gtcgattaac gggttgccgg ctgggttttt
     7741 ccagtacacc acaatcgcaa ttaacacacc agcaaggaat aacaacatcg gcggcgcacg
     7801 gtgacctttg aatactttgt cgcttaaata accgcttgca agcataccga agataccggc
     7861 atattcatac aagaagtacg cccagctctg tttatccaca gagaaatgtt tcacttcttt
     7921 taagtaagtc ggtgcccagt cgataatgcc gtagcgaatg aaatagacaa acacgttggc
     7981 aatcgcaatt gcccacagga atttgttgtt taaaatatat ttgtagaaaa tgtctttgga
     8041 agagagtgtg tgggcagact ccactttttc caccactttc tcgcctttgt actcatcaat
     8101 cggcggtaag ccttgagatt ccggcgtatc acgcatcaaa tagaacatca caaacgccag
     8161 tacaatcgca atcaacgccg gcagatagaa cagggattgc cacacgccaa aaatagataa
     8221 gcctaaaatc gccaacggcc cgattaaacc gccgcccaag ttgtgcgaca cgttccacca
     8281 gctccaccac acgccacgtt cagaggttga gaaccagttg gtcatcgttt tcgcccccgg
     8341 cggatagccc attccttgga accagccgtt taacgccgcc aaaatgatca taattggaat
     8401 agacgaaagc acgcccggta ctaaaccgaa caccagactc acaatcgctg aaccgagcaa
     8461 gccgatagtg ataaagtatt tcgggttaga acggtcggaa acgttaccca taataaattt
     8521 actgaagccg taagcgagtg ataatgccac gcccaccgta cctaagtctg ctttggtgaa
     8581 gccatattca tcaatcaaat acggcattgc caacgagaag tttttacgaa ttaaataata
     8641 tgccgcatag ccgataaaga cgcctgcaaa cacttgccaa cgtaatttgc gatattccgc
     8701 atcaactctg ctggcttcca ctttttgttc tgctggtgga gaggctttta gaaaggaaaa
     8761 cattttacta ctcctttaag aatgaaatcc tatggctact ataatctttc atttggagca
     8821 gaaaaagtgg atttttgaaa atttgtgagc aagattaaaa ttattttgag cgaaaacgaa
     8881 aagcggttga atttccttct ttttttgcaa aaaatttgag aaatctaacc gcttgtttca
     8941 cttaaaaatc agctatttct gctttttcaa aaattccacg cccgtatcag ggaagtcagt
     9001 aaagacgcct gttgcacccg ctttattcag cagggcatcg tacatttcat cgacattttg
     9061 gaaaaagtcc ggcagagcat ctttgcgcac ggtgtatggg tgaagttcca ttttgtactt
     9121 cgccaactct ttcactaatg gcgtgtaaac aatgttgccg actttagatt tttctttatc
     9181 caccaacata taccagcccg ggccgacacc atcggcatat tttgccactt ctgccatcgc
     9241 accgtctttg aacatccagt cgtagtcgta attaacccag tttctcttcg gtttcgtgcc
     9301 aatcggtgta ggcaactaac tgcaccaatt ttacgtccat acccagtttc ggtagtaatt
     9361 cggttttgat ccgtttcagc tcgttgaaat cgaaggtttg caggtaaatt ggtgaggttt
     9421 tgctgtcgta gccatatttt ttcaacactt tcagggtttc gagtgcaatg tctttaccct
     9481 ctttatggtg caaccaaggg gctttaattt caggatagat cccaattttt ttgccggttg
     9541 atttctctaa tccttgaata aattccagct cttcttccaa agtgtgaatg gtgaaatggg
     9601 actgccacat cgggaagcgg ttcgggtaaa cttgtgcctg cttgccgtct ttcacctcaa
     9661 aattctcggt catttcaagg gttttaatct catcaagggt gaaatccacc acataataac
     9721 gaccgtctgc tcgtttacgc tcagggaatt ttttcgccac atcagttaaa ccgtctaaaa
     9781 agtggtcgtg aatcacaatc aggcggttat ctttggtcat agcaagatct tgctctaaat
     9841 agtccgcttg ttgcccgaat gctagagctt tagattcaag cgtatgctca ggtaaataac
     9901 cgcttgcacc acggtgggca atcactaatt tatcagattg ttgtgcatag gcacccaatg
     9961 ctgtcgcgga caatgctaat gcagcaacca atgttttgaa tttaaatttc atagaattac
    10021 ctcatataat gtaaaaataa aaggaagaac aaaaatgtcc ttccttttga taagttattt
    10081 attatttacc atagtgatct tctaattttg ctttgtgctt gccttcttca accatcacaa
    10141 tgaaggttaa gagaatggct aatacaccac caccgatcat cacgtagaaa ccgccgtccc
    10201 agccaaattt atctgctgcc cagccaacta ctgcagaggc tgccactgta ccacctaagt
    10261 aaccgaataa accggtgaaa cctgctgctg tacctgctgc ttttttcggt gcaagttcaa
    10321 gggcgtgtaa gccaatcaac attaccgggc cgtagattaa gaaaccgata gtggtcatcc
    10381 aaatgaagtc cattaattga tatgggtttt cataccaagc atggcctgca tattgtgcaa
    10441 tttcagattc cggtgtcgca gggttgagcc agaacgcaac cactgctgcg gtggtaagaa
    10501 tcataaagat gaaaccggtt aaaccacgtt tgcctttgaa cagtttgtca gagacccaac
    10561 cacagagtaa tgtacctgga atcgcagcta attcataaat ggtatatgcc catgctgtac
    10621 ctttaatgtt gaagtgtttt acttcgccta agtacaccgg cgaccatttc aatacgccat
    10681 agcggattaa gtaaacgaag acattcgcaa tggcaatgta ccataacagt ttgtttttca
    10741 gcacataagt cacgaaaatc tctttggtgc ttaaatcgtg ttcataggtt ttttcgttat
    10801 agtcgtcagg gtagtcgtta cgccattttt caactggcgg taaaccgcaa gattgtggag
    10861 tatctttcat cacaaggtag attggaatag ctgcgatcat tgctgcaatt cccgggaaat
    10921 aaagggcttg ttgccataca tcttttgccg ttgcttctac accatactca ctgaaataga
    10981 gcgcacttgc taatagcacc atcgcccccg gcaccatacc gcctaagttg tgagcggtat
    11041 tccagataga aacgatagta ccacgctcag atttagacca ccagtgaacc attgttcgtc
    11101 cacacggtgg ccaacccata ccttgaaacc agccgtttaa gaagatcatt acccacatta
    11161 ctaaaatgct tgatgttgcc caaggcatta agcccataag ggtcatacat agacctgaaa
    11221 gtaacaatcc ggtcggtaaa aatactttcg ggttagagcg gtcggacatt cctgccatca
    11281 cgaattttga taaaccgtag gccaggccgg cagctgtacc aatcacaccg agatcggttt
    11341 ttgtatacat accagcttca atcaagcctt tttgtgctaa gtcgaagttt gcacgcacaa
    11401 aatagtaagc ggcatagccg aagaaaattc ctgcaaacac ttgccaacgc aagaatttat
    11461 atcttgcatc gactttgtcc gccggtaatt ccgcaatgtg cggagcgggt ttaaatggac
    11521 caaacatagt attctccaat attacttagt aaaacctctg agtaattcct ttttaggaaa
    11581 ttagggaagc aataataaat gaaaataaaa aatgacaaag ttatttttta taaaaatgtg
    11641 attcatttca caaaaaattt accttaaacc acaaaaaatg attgaaaaag aaaatcaaac
    11701 atcagtatct ctagcgataa aactctcttc ttatgtacag agaaagaacc ataaaaagta
    11761 caatatagaa atattttgtg acatagatct aatttttaaa caaatgtctt tgaagtcatt
    11821 ttattttcgt ttatgctaat taagtaatga ttaaatgttt taattcgagc aaaaggagca
    11881 aaaaatgcaa agacatgaac agctagctca agtacaacaa acgcaaaaat gggattttat
    11941 tattatcggt ggtggcgcaa gtggtttggg gattgcggta gatgcagctt cccgtggcta
    12001 taaaacctta ttattagaaa gccatgactt tgcgaaaggt acatcgagcc gaagcacaaa
    12061 attagtccac ggtggcgttc gctatcttgc aaatggcgat gttgcactcg tgagagaagc
    12121 attgcgtgaa cgaggtcgtt tagctaaaaa tgcagctcat ttatttaaaa accaaagctt
    12181 tattattcca aactacaaat tcaaagactg tgtaatgtat cgtgttggtt taggcttata
    12241 cgatatgctt tcaggcaacc tgagtttagg aaagactgaa aacatcagta aaactgaagc
    12301 gaaaagacgt ttaccaacct taaatgatac tggtttacgt acaggcactg tttatcaaga
    12361 tgggcaattt gatgactcac gtcttgcagt gaatatggca caaacggtag tagagcacgg
    12421 cggtacagta ctaaatcacg ccaaagtgac ttcactgcac aaaacgccac aaggcaaaat
    12481 tgatggggtg actttctacg atgaactcaa tcaacaagaa catactgttt tcggcactgc
    12541 ggtgattaac gcaaccggcg tatttatgaa tgatatttta agtatggatc acggtagcac
    12601 gaaaaaattt gtcgtaccaa gccaagggat tcacttagta ttagataaat cattcttacc
    12661 aagtaatgat gcgttaatga ttccaaaaac ctctgacgga cgagtgttat ttgctgtacc
    12721 ttggcacgaa gtgttagtcg tagggacaac agatactctg attgaaaatc cttcttatga
    12781 gccaattcca ttggaacaag aggtgaactt tattttagag acagccgcac aatatctgac
    12841 aaaaaaacca actcgtgatg atgtaaaagc agtctttgcc ggtcaacgtc cgcttgcggc
    12901 accggaaaaa gaaggtcaaa gcactaaaga agtatctcgt agtcacaaag ttctcactga
    12961 tgcaaccagc ggtttgatta cgattacagg tggtaaatgg acaacttatc gtcaaatggc
    13021 agaagatacc gtagaagaag cgttaaaagt ccactctgaa ctaggcaata aaccttgtgt
    13081 gaccgtcaac ttaccgattc acggtaatat tccggcagca caagtgaatc tacaagatca
    13141 tctatatgta tatggctcag atattccaca gttacgcgcc ttaatgactg agaccccgga
    13201 atatgcacaa aaaatccatc cgagactaga ttataccgtt gcagaagtga tctgggcagt
    13261 tcgccacgaa atggcacaaa cggttgagga tgtattagct cgccgtgttc gtttgctctt
    13321 cactgatgcg agagcggcga ttgatagtgc agcaaaagtg gctgctatta tggcgaaaga
    13381 attaggtaaa gatgaaatgt ggcaacaaca gcaagttatc cagttccttg atgttgcgaa
    13441 gcactattta ttggtagatt atcagccaca gccaatctaa tacgctaaaa gcaagcggtt
    13501 aaaatcgata aaaatattgc aattacgtta tttgcaactt tttaaaccat tttaaccgct
    13561 tgtatattag ctatcatctc ttttatgttt ctgttgtaat aaaaagaatg ctgttaaccc
    13621 caatacagaa aagctaattc ctttcatact caataatctt tttaccaaaa aatctaatgc
    13681 aatggtttta actgccggtg aattgaccag tgcaagccct gtttgtttca cttgtttaaa
    13741 aggctgcata atctcatttt tggttttatt ggtttccgct ttaaatcgca ttctaagtgc
    13801 attacctttt agaactagaa gttccttttc ggttaattta agctcttcca ttttaatgtt
    13861 ctccattctg tttcaaagtt aaagcactct taaatgcggc aatatctaat tttagctcgg
    13921 ttaatgtact ttgcataaaa tggcgttgtt ttttcaaggc agaaagtatc atcattccgc
    13981 aaatagtcac agcaataaac ataataatgg caatagaaaa gaatacttta actttctgtt
    14041 cgggtaacaa ataattatct aaaccaaata ataaactcac aaaagaaact aataaaaata
    14101 caaaagccaa taagataaat ataagtactg aaattaattg atttttttgc tcattaattt
    14161 caattcttgc catctcacca cgcacttgag ctatttccaa taatgtaatg agactatttc
    14221 taaatccgga tttaatatta tctaacatta taaccattcc ttatattaaa taataagcca
    14281 ccccaaatat ggcgtggctt aatgacttaa tgacgaatta cagataatta tcttctagaa
    14341 attaaaacac ctaataataa ccctaccaca ccggcaacgg taattgcttt atacggatta
    14401 tcttgaacta aggtatcagt ttgtttaact acagttcttg cactatcaac ggtatcatct
    14461 tttaatgttg caaaacgatc ttttacatca tcaaactgtt ttgctaattt atcttttaac
    14521 tttttatatt cctccgtaga agaatcagat gtttcattaa ataattcctc tgcatttttc
    14581 aaaatatcgc gaatctcgtt tgttaactct tcacgttttg catcta
//
LOCUS       DANLXS010000051        14609 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.051, whole genome shotgun sequence.
ACCESSION   DANLXS010000051 DANLXS010000000
VERSION     DANLXS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14609
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.051"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            73..1581
                     /locus_tag="PXD71_001116"
     CDS             73..1581
                     /locus_tag="PXD71_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL6291249.1"
                     /translation="MSNQSSNAPQRETFSGRGAFIMAAIGSAVGLGNIWRFPYTTYEN
                     GGGAFILPYIIALLTAGLPLLFLDYSIGHKYRGGAPLAFRRFSSKFETFGWWQVLINV
                     IIGIYYAVVLGWAASYTYFSFSGAWGDKPVDFFIGEFLKMGDITQGVSFEFVGMVTGP
                     LIAVWLIAIAVLAMGIKKGISAASNLLMPQLIVMFLVLVGYSLTLPGAAKGLDALFTP
                     NWEKLADPSVWIAAYGQIFFSLSICFGIMITYASYLKKETDLTGSGLVVGFANSGFEL
                     LAGIGVFAALGFMATAAGQEVNEVAKGGIGLAFFAFPTIINQAPMGALVGVLFFGSLT
                     FAALTSFISVIEVIISAVQDKLNSGRVKATLLVGIPMMVVSVLLFGTTTGLPMLDVMD
                     KFVNQFGIVAVAFVSLIAIVTSEKLSTLEKHLNEVSSFKVGFIWRLCIVITSGVLAFM
                     LFSEIAKVYREGYEGYPSWFVNTFGWGMAIGLVVVAYLLSKMAWKNEPKGDE"
     gene            1581..1679
                     /locus_tag="PXD71_001117"
     CDS             1581..1679
                     /locus_tag="PXD71_001117"
                     /inference="COORDINATES: protein motif:HMM:NF028261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HDL6291250.1"
                     /translation="MSSAAIITMVVAMTIIWGGLLFAIKRLPKEEQ"
     gene            complement(1727..1990)
                     /locus_tag="PXD71_001118"
     CDS             complement(1727..1990)
                     /locus_tag="PXD71_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDL6291251.1"
                     /translation="MFVEIYGRLSCPYCVRAKTLAEKMKQELSDFDFKFIDMIAENIS
                     KQDLEPRVGKPVATVPQIFVDNNHVGGCTDFQAFVEEKFGITV"
     gene            2150..3484
                     /gene="srmB"
                     /locus_tag="PXD71_001119"
     CDS             2150..3484
                     /gene="srmB"
                     /locus_tag="PXD71_001119"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDL6291252.1"
                     /translation="MTQLMTFEELDLSPQLLKALAHKGYNRPTAIQQATIPAALEGRD
                     LLGSAPTGTGKTAAFLLPAIQHLLDYPRRKPGAPRILILTPTRELAMQVAEQAQALAK
                     FTQLSIATITGGVAYQNHGEVFNKNQDIVVATPGRLMQYIKEENFDCRAVEVLIFDEA
                     DRMLQMGFGQDAEKIAAETRWRKHTWLFSATLEGELLEDFAERLLNDPIKVDAEPSRR
                     ERKKIQQWYYHADNVEHKTKLLARIISEFSVERAIVFVRRREDVRELSETLRKRGIRS
                     TYLEGEMAQTQRNQAINRLKEGVVNVLVATDVAARGIDIDDIDFVLNYDLPYSADTYL
                     HRIGRTARAGKKGSAISLVEAHDYKLLGKIKRYTEEVLKPRIIEGLEPRTKAPKDGEL
                     NVTSKKEKARIKKKKEAKKEAAKPKVKVRHKDTKNIGKRRKPSSESAAVKQA"
     gene            complement(3553..3846)
                     /locus_tag="PXD71_001120"
     CDS             complement(3553..3846)
                     /locus_tag="PXD71_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL6291253.1"
                     /translation="MSKTMQQKAAEAHNMCRLKGDSMLDNADRQIAFEAVYESEELAQ
                     QAVEFFAQKAKSVETEPCEIQSEITQIAEGFLMKMWITFSCQAEVILFQMAVR"
     gene            complement(3848..4510)
                     /locus_tag="PXD71_001121"
     CDS             complement(3848..4510)
                     /locus_tag="PXD71_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL6291254.1"
                     /translation="MPFPDEISELKGYFDGTNPVSAYPAQGLNVPFYILGSSPESAYL
                     AAERGLPYAFASHFAPRFMDDAIRIYRSHFKPSDVLDKPYVILGVNAIVAETDEEATQ
                     LATTQTQFFLNVVTNAQQNLQPPLESDDKVWENFVKAEIVPHFGPVAFEQNALYRQEK
                     TVVRQMTECSLIGSPQSVEQQLKALKARVEIDEIMAVNYIFDEQKQHQSYTWLKQITD
                     KV"
     gene            complement(4494..4946)
                     /locus_tag="PXD71_001122"
     CDS             complement(4494..4946)
                     /locus_tag="PXD71_001122"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HDL6291255.1"
                     /translation="MVLQVLNSKAIQPFLYANSENKMTNLSILNLVPIREGQTAKQAV
                     DSMVKLAKHAENIGIERYWIAEHHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLP
                     NHSPYIVAEQYGTLETLYPNRVELGLGRAPGTDMRTASALRPEFAFPR"
     gene            complement(4966..5604)
                     /locus_tag="PXD71_001123"
     CDS             complement(4966..5604)
                     /locus_tag="PXD71_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL6291256.1"
                     /translation="MKKFALKTVLVALSALFAFNATAVAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELNYKIPSQIKAKLPADSKLVQYHVNFLGRQSENLTRAWAL
                     AMALGAEDKVKTALFEAAQKDAFKSMDDIRAVFIANSISAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFSDSSSTNDFIQRYIDAVVFLSKK"
     gene            complement(5648..5917)
                     /locus_tag="PXD71_001124"
     CDS             complement(5648..5917)
                     /locus_tag="PXD71_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDL6291257.1"
                     /translation="MKCKRLEELLELLGEHWRKNPDLHLIDILQQLSVEVSEPDNFHA
                     LRDEVLIYQLKMRNADKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            6009..7118
                     /gene="mobA"
                     /locus_tag="PXD71_001125"
     CDS             6009..7118
                     /gene="mobA"
                     /locus_tag="PXD71_001125"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL6291258.1"
                     /translation="MEEKISAVILAGGLARRMNGAEKGLQLLDQKPLISHLLARLSPQ
                     IEDIWLNINRSVEQYQQLYPELPYYQDSLPDFQGPLSGMLSGFEQIESDYLLFVPCDT
                     PFIPTQLLHKLHTALRINQAQIAYAHDGDRPHPTVALVHRSVAENLRAYLASGERRLL
                     WFFQTQKSVAVDFSAYKAAFRNFNCLEDLTAFNPYPIKTLAITGYSGTGKTTLLEKLI
                     PKLTACGIRVGLIKHSHHNVDIDKKGKDSYRLREAGANPTMVVCDERWALMTETKQAA
                     ETKQAVEFSQLIAKFDPETVDLVLVEGFKHETMPKIQLHRKEIEKPLPALDQWTIATA
                     TDYPLERENRLDINDVEQLADFIRQWLEASKEPAN"
     gene            complement(7173..11684)
                     /locus_tag="PXD71_001126"
     CDS             complement(7173..11684)
                     /locus_tag="PXD71_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6291259.1"
                     /translation="MINKNFPLSYLASFVAFAVSLSATASVFRDDIDLQYYRDFAENK
                     GKFTVGASNIEIKDKSGNTLDALFPNVPMPDFSAANRNLGIATLVAPQYLVSVAHNTQ
                     YNTVEFGAPGTNADAHHYTYKVVDRNDYGIVEGGQHQDYQVPRLNKLVTEVAPATVTD
                     LGNNASAYQDSSRFTHFARLGSGRQIVKNTEHKDNQISTSYQYLTGGVHLPIAVHNSD
                     YWLDFRGNALNNSPYGALTAFGTRGDSGSGVYGYDQKTKRWLLLATYTFGTPANNYYN
                     RAGIIRQDYHDKQFAEDIAGTLTNAQQNAVFEWSAQGKDSSIGNKGKNLTVSLADRSI
                     KDNSASPLGYGGTPQLQLPQDNTGKTLNIEGQDSTIVLKTDIDQGAGALNFNANATVR
                     PENDQSWVGAGIVVAKGKQVNWQVKNPQGDRLSKLGEGTLHINGKGENLGDISVGQGT
                     VILTQQAGENGKKSAFNQVGIVSGRSTVVLNSADQVDPNKIYFGFRGGRLDLNGNNIA
                     FNRIQNSDDGARIVNNHLQKAATLTINGPKPPEATDLKWGTWKENSADIYEYINPHAN
                     NRTDYFTLKGNPNQYMPTNGASNAHWTFLSSNKDAAVKQVLAQKGLEHRYNSFNGFIG
                     ETDNTQHNGRLNVVYDPKASTPPATASEVTWGKGLVAGADIYLFTNPKTKIREYFALK
                     GDPKQPVPRGGLSSEHWEFLAADRNQAINKVLARKNAPIEQEKLNSIYTFSGGLNLNG
                     DLTVKGGKVLLSGRPTPHAYDAINKQDVVYADDWQNRQFKADNMQLNQYAQLYVGRNV
                     SNLQANLSANDHAQLHLGFINEQTPSCYYSEYTGKTSCDTQAVVSNEIFATLPTTQIN
                     GDVSLAEQSQLHIGKANLIGTIQAAATTSIRLANQASWTNTGDSRTGNLVAENGSTIN
                     LNEKFVTGEIPTRFNTLIIDGNFQGNAKINYLTDIAAGKGDHLQVNGLAEGTFTLALR
                     NSGKEAEVVSPLSLLTLTHQAQADKAKVSLENGYVDLGAYRYVLANRSNGYRLYNPLK
                     DAKDRNQSIATAKAELDHAIAEADKQKQEISRLNAEAEKERQAEKNAKQAAANAQSQL
                     SQANSELTRLQQYADYYRRYYPTYYRQIQGQITVAKQKVTQASAALTTAENNAKASAA
                     QIANVEQAVETAQNIAKQVEAKLATLRITAGNSEAILNAEALKLCQENGANCEHLQAH
                     ADTDSSNIQQSDWVSQYANTALSELSAQANSALQIGQDLDRQLFAKHDKFHVWSSIEH
                     QKTEHQSDLYRPYEQQTNLTQLGIELPLANGINAGVMLSRNHANAEFDEGVNGKSNLL
                     MASLYGKWHSENGTFVSLDGSYGKAKNRIDLFGENRFNRHIMAIGANLGHNFDLAGVQ
                     VQPAVGTRYYRFSAQNYKLGEVEVRSPKAHFMAYQAGVKVSKAFDLSSWKVEPSLAAH
                     YVDASSKRLSVAVNDNTFSQRFGRYLKTEVGVGVTKGQWQLSSHLGLLKGNEIGKQHF
                     AGFKLGYSW"
     gene            complement(11868..12872)
                     /gene="fbp"
                     /locus_tag="PXD71_001127"
     CDS             complement(11868..12872)
                     /gene="fbp"
                     /locus_tag="PXD71_001127"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDL6291260.1"
                     /translation="MKTLGEFIIEKQAEYPGAKGELSGILSSIRLAAKIIHREINRAG
                     LSQDILGTAGSENVQGEAQMKLDVFANETMKKALLAREDVAGFASEEDDNFVAFDNER
                     GRNAKYILMTDPLDGSSNIEVNVSVGTIFSIYRRVSPIGTPVTQEDFLQEGRKQVAAG
                     YVTYGSSTMLVYTTGNGVNGFTYDPSLGLFILSHPDLKTPTEGKYYSINEGQYVTFPQ
                     GVKRFIKYCQETDEAIKRPYSSRYIGSLVSDFHRNLLKGGIYIYPTSTVYPKGKLRLL
                     YECNPIAFLAEQAGGMATDGINPILDIKPTELHQRVPFFVGSTSMVKQAGEFMQEFAK
                     "
     gene            12996..13787
                     /locus_tag="PXD71_001128"
     CDS             12996..13787
                     /locus_tag="PXD71_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL6291261.1"
                     /translation="MNNLRIKMISPEIALAKYNELDVLEINHSKVKAKIALQGAQLLS
                     WQPKNTQQDVLWLSEIEPFQTGVAIRGGIPICHPWFGTDKKPAHGTARIQAWNLLEHT
                     SSADGIRVVLGLENQAKLEMILGEVCELYFTHLAPEPAQAALHTYFQVGDITKTEVQG
                     LATQCFDKLTNQEIEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETV
                     LWNPWHNTVSAMTETAYQNMVCVETARINTLLQQNETLGVRILVR"
     gene            complement(13855..14544)
                     /locus_tag="PXD71_001129"
     CDS             complement(13855..14544)
                     /locus_tag="PXD71_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDL6291262.1"
                     /translation="MLQLRKANERGDGSFSWLKSYHTFSFANYYDLKHIHFSHLRVIN
                     EDFIAPNHGFGKHPHDNMEILTYMLKGRVAHEDSMGNKTEVKAGEFQIMSAGTGVFHS
                     EINPDPNETLHLYQIWIIPNQKDVTPRYNQDRFAPKEGATLILSPNAEEGSFKIYQDM
                     KLWRYQYPTAKSEQLALEPNRSYWLQVVKGNLNINGVELSTSDALGIRQEKALEINTQ
                     DDVEFLLFDLV"
BASE COUNT         3931 a         2990 c         3251 g         4437 t
ORIGIN      
        1 ttaaagtgtg attaatttac atttaactca attctttttt atataaaact ttatcagttt
       61 gaggtattta ccgtgtctaa tcaatcatca aacgcaccgc aaagagaaac gttctccgga
      121 cgtggtgcat ttattatggc ggctattggt tccgctgtcg gcttagggaa catctggcgt
      181 ttcccttata ctacttatga aaacggcggt ggggctttta ttctgccgta tatcattgcc
      241 cttttgaccg caggtttacc gctgctgttc ttagattatt ctatcgggca taaatatcgt
      301 ggtggtgcac cgctcgcttt ccgccgtttc agctcgaaat ttgaaacctt cggttggtgg
      361 caagtgctga tcaacgtgat catcgggatt tattatgcgg tggtactcgg ctgggcagca
      421 agttacacct acttctcatt cagcggagcg tggggcgata aaccggtgga tttctttatc
      481 ggtgaattct taaaaatggg cgatattact caaggcgtga gctttgaatt tgtcggtatg
      541 gttacagggc cgttaattgc ggtatggctg attgctattg ctgtgttggc tatggggatc
      601 aaaaaaggga tttctgctgc ctcaaacctg ctaatgccgc agttgatcgt gatgttctta
      661 gtgttggtcg gctattcatt aaccctaccg ggtgctgcga aaggcttaga tgcgttattt
      721 accccaaact gggaaaaatt ggctgatcca agtgtgtgga ttgccgctta cggtcaaatc
      781 ttcttctcac tctctatctg cttcgggatt atgattacct atgcttcata cctgaaaaaa
      841 gaaaccgatt taaccggttc aggcttagtg gtgggttttg cgaacagtgg ttttgaatta
      901 ttagcgggta ttggcgtatt tgcggcactc ggctttatgg caacggctgc agggcaagaa
      961 gtgaacgagg tagcaaaagg cggtatcggc ttggcattct tcgctttccc aaccattatt
     1021 aaccaagcac caatgggggc attagttggc gtgctgttct ttggttcact cacctttgcg
     1081 gcattaacct catttatctc ggtaattgaa gtgattattt cagcggtgca agacaaactc
     1141 aattcaggtc gtgtgaaagc cacgctacta gtcggcattc cgatgatggt ggtatctgtc
     1201 ttgctattcg gtacaaccac cggtttacca atgcttgatg tgatggataa attcgtaaac
     1261 caattcggta ttgttgcggt ggcgtttgtt tccttgattg cgattgtcac cagcgagaaa
     1321 ttaagcaccc ttgaaaaaca cctgaacgaa gtttcttcat tcaaagtcgg ctttatctgg
     1381 cgtttatgta tcgttattac ctccggcgta ctggcattta tgttattcag cgaaatcgcc
     1441 aaagtgtatc gtgaaggtta tgaaggctac ccaagctggt ttgtaaacac cttcggctgg
     1501 ggaatggcaa tcggcttagt ggtggtggct tatttgctat caaaaatggc gtggaaaaat
     1561 gaaccaaaag gagatgagta atgagttcag cagcaattat taccatggtg gtggcaatga
     1621 cgattatctg gggcggttta ctttttgcga ttaaacgtct gccgaaagaa gaacaataat
     1681 ttgcaaattt ttgtataaaa aagcccgctt gttagcgggc ttttggctat acggtaatcc
     1741 caaatttctc ttccacaaag gcttggaaat cggtgcaacc gccaacgtgg ttattatcca
     1801 caaaaatttg tggtacagtt gcaaccggct tgccaacacg aggctctagg tcttgttttg
     1861 aaatattttc agcgatcata tcaataaatt taaaatcaaa atcgcttaat tcttgtttca
     1921 ttttctcggc taatgttttt gcacgcacgc agtatgggca agataaacgt ccataaattt
     1981 caacaaacat ataatgttcc ttctataaag taaaagtgag cgaattgtcc gttaaacggc
     2041 tctgtgtaag tgtaacaatt ttttcaacct tttaggataa atttcttcac catcacggca
     2101 agcagttaat gtgatatact ggggcaaatt tacaagtaga gaaacagaga tgacccaatt
     2161 aatgacgttt gaggagcttg atttaagccc tcaattactt aaagccttag cacacaaagg
     2221 ctataaccgc ccaaccgcta ttcaacaagc cacgattccg gctgcgttag aaggacgtga
     2281 tctgctcggt tcagccccta ccggtaccgg caaaaccgcc gcttttttac tgccggcaat
     2341 tcagcattta ttagattatc ctcgccgcaa accaggggct cctcgcattt taattcttac
     2401 cccaacccga gagctggcaa tgcaggtggc ggagcaagcc caagcactgg ctaaatttac
     2461 tcagttgagc attgcgacta ttaccggtgg ggtggcgtat caaaatcacg gtgaagtctt
     2521 taataaaaat caagatattg tggttgctac cccgggtcgt ttaatgcaat acattaaaga
     2581 ggaaaatttt gactgccgag cggtggaagt gctgattttt gatgaagcag atagaatgtt
     2641 gcaaatgggc ttcgggcaag atgcggagaa aattgcggca gaaacccgtt ggcgtaagca
     2701 tacttggttg ttttcagcga ccctcgaggg cgagttatta gaggattttg ccgagcgatt
     2761 attaaatgat ccgattaaag tcgatgccga accaagtcgc cgtgagcgta aaaaaattca
     2821 gcaatggtat taccacgccg ataatgtaga gcataaaacc aaattattag ctcgtattat
     2881 cagcgaattt tcggtggaaa gagcgattgt gtttgtccgc cgccgagaag atgtgcgaga
     2941 actcagcgaa accttgcgta agcggggaat tcgttctact tatctcgaag gtgaaatggc
     3001 tcagacccaa cgtaaccaag ctattaaccg tttaaaagaa ggcgtagtga atgtgttagt
     3061 ggcaaccgat gtggcggctc gaggcattga tatagacgat attgatttcg tgctgaacta
     3121 cgatttacct tatagtgcgg atacttactt gcaccgtatt gggcgtaccg ctcgtgctgg
     3181 caaaaaaggt tcggcaattt cattggtgga agctcacgat tataaattac tcggcaaaat
     3241 taaacgttac accgaagaag tactaaaacc tcgcattatc gagggcttag agcctcgcac
     3301 caaagccccg aaagatggcg agctaaatgt gaccagcaag aaagaaaaag cacgcattaa
     3361 gaagaaaaaa gaggcgaaaa aagaagcggc aaaaccaaaa gtaaaagtcc gccacaaaga
     3421 taccaaaaat atcggtaaac ggcgtaaacc aagtagtgaa tcggctgcgg ttaaacaggc
     3481 ataatgatac aagcggttat tttctgaaga ttttttgcaa aaatttcatc aaaaataacc
     3541 gcttgtttat atctacctta ctgccatttg gaacagaatc acttctgctt ggcagctgaa
     3601 agtgatccac attttcatca gaaacccttc tgcaatttgt gtaatttcgc tttgaatttc
     3661 acaaggttcg gtttcgaccg attttgcttt ttgtgcgaaa aattcgaccg cttgttgagc
     3721 cagttcttcg ctctcataca ccgcttcaaa ggcaatttgg cggtctgcat tatccaacat
     3781 agaatcgcct tttagccggc acatattatg tgcctcagcc gctttttgtt gcatcgtttt
     3841 gctcatatta caccttatcc gtaatttgtt ttaaccaagt gtaggattga tgctgttttt
     3901 gctcatcgaa aatgtagttt accgccataa tttcatcaat ttccactcgt gcttttaggg
     3961 ctttaagttg ttgttcaacg ctttgcgggc taccaattaa ggagcattcg gtcatttggc
     4021 ggacaacggt tttctcttgt cggtatagag cattttgctc aaaggcaacc ggcccgaaat
     4081 gcggcacaat ttctgctttc acaaaatttt cccacacttt gtcgtcagat tcgagcggtg
     4141 gttgcagatt ttgttgggca ttggtcacta catttaagaa aaactgggtt tgggtggtcg
     4201 ccagttgtgt tgcttcttca tcggtttctg ccacaatggc attcacacct aaaatcacat
     4261 aaggtttatc taacacatca gagggcttaa aatgtgagcg gtaaatgcgg attgcatcgt
     4321 ccataaagcg aggggcaaag tgtgaagcga aggcgtaagg caagcctcgt tcagccgcta
     4381 aataggcact ttcagggctt gagcctagaa tataaaacgg cacatttagc ccttgtgccg
     4441 gataggcgga aacagggttt gtaccgtcaa agtagccttt taattcgctg atttcatcgg
     4501 ggaaaggcaa actctgggcg taaggcagag gcggttcgca tatcggtgcc gggggctcgc
     4561 ccaagcccca gttctactcg gtttgggtag agtgtttcaa gcgtgccgta ttgttctgcc
     4621 acaatataag ggctgtggtt gggtaacatc acgccaccgg agccgactct taatttttcg
     4681 gtgtgggaga gggcgtgttg aatcaataat tgcgtggcgg aactggcaag atttttcata
     4741 ttatggtgtt cggcaatcca atagcgttca atgccgatat tttctgcgtg ttttgctaat
     4801 ttcaccatag aatcgaccgc ttgtttggca gtttgccctt cacgaatcgg cacaaggttg
     4861 agaatggata agtttgtcat tttgttctcc gagtttgcat ataaaaaagg ctgaatagct
     4921 ttactattca gcacttgtaa aaccatctcc ttaatatggg gacgattatt tcttactcaa
     4981 gaagaccaca gcgtcaatat agcgttggat aaaatcattg gtcgagcttg aatcggaaaa
     5041 tccttctaga ttaagctggt attgaccatt cacgaaaaac gccggtacac cacgaatttg
     5101 gaagtcttca gctaattgaa cttgtttatt gactaaaccg tttactgcaa agctgttgat
     5161 accgctgtca aactgttctg cactaatgct gttagcgatg aagaccgcac gaatatcgtc
     5221 cattgattta aacgcatctt tttgagcggc ttcaaacaag gcggttttta ctttatcttc
     5281 cgctcctaat gccattgcca atgcccaagc acgggttaag ttttcagatt ggcgacctaa
     5341 gaaattgacg tgatattgca ctaatttgct gtctgcaggc aatttcgctt tgatttggct
     5401 tggaattttg taatttagct caaaatcgta acagtggggg cagtagaatg agaaaaattc
     5461 caccacttct ttttgagccg atggggcttt acgcacctca atatactctt tgccggcagt
     5521 tggatctgct gcaactgcgg ttgcgttaaa tgcaaaaagg gcagaaagag caaccagtac
     5581 ggtttttaat gcaaattttt tcataatgat tccttcaaaa atatgataag tgagaaataa
     5641 gacaaactta atcgtttaaa attccacgag ctcggagcaa tgcggtttta aaatcatctt
     5701 cataatcttt tttaatgccc ggaatcggct caaatttatc ggcgtttcgc attttgagct
     5761 gataaatcaa aacctcgtca cgcagggcat gaaaattatc cggctcgctt acttccaccg
     5821 aaagctgttg cagaatatca attaagtgaa gatcggggtt tttccgccaa tgttcgccaa
     5881 gcagttccaa tagctcttct aaacgtttgc atttcatagt atgcctctta aaaaacttgt
     5941 tgaatagtaa gcggaataat atacttttag ccctcatttg gcaaaaaaat tcaggtttgg
     6001 agagggaaat ggaagagaaa atttcagcgg taattttagc cggaggtttg gctcggcgaa
     6061 tgaatggagc ggaaaaaggg ttgcaactgt tagatcaaaa accgttgatt tcgcacctgt
     6121 tagcccgact ttcgccacaa atcgaggata tttggctcaa tatcaatcgt tcggtggaac
     6181 aatatcaaca actttaccct gaattacctt attaccaaga cagcttgccc gattttcaag
     6241 gcccactaag tggaatgctt tccgggttcg agcagattga gagcgactat ttactgtttg
     6301 tgccttgtga cacgccgttt attccaaccc aactattgca caagttacac acggcattac
     6361 gcattaatca ggctcaaatt gcctatgctc acgatggcga ccgcccacac ccaacggttg
     6421 ccttggtgca tcgttcggtg gcggaaaatt taagagcgta tttagcctcc ggcgaacgcc
     6481 gattattgtg gttttttcaa acccaaaaaa gtgtggcagt ggatttctcg gcatataaag
     6541 ccgcatttcg caattttaat tgtttagaag atttaacggc tttcaatcca tatcccatca
     6601 aaaccctggc gattactggc tatagcggca caggcaaaac caccttattg gaaaaattaa
     6661 tcccaaaatt gaccgcttgc ggtattcggg ttggcttgat taagcattcc caccacaatg
     6721 ttgatattga taaaaaagga aaagacagct atcgcttgcg agaagcagga gcaaacccga
     6781 ctatggtcgt gtgtgatgaa cgttgggcat taatgacgga aactaagcag gcagcagaaa
     6841 ctaaacaagc ggtcgaattt tcacaattaa ttgcaaaatt tgacccagag acggtagatt
     6901 tagttttagt ggaaggcttt aagcacgaaa caatgccgaa aattcaacta caccgtaaag
     6961 aaattgaaaa accgttgccg gcgttagacc agtggacaat cgcgaccgca accgattatc
     7021 cgcttgagcg ggaaaatcgg ttggatatta atgatgttga acagcttgct gattttattc
     7081 ggcagtggct agaggcaagc aaagagccgg ctaattaatt gactaaaaag cccctaaagt
     7141 aagcgattgc ttagtttagg ggctaatagc aattaccagc tatatcccag tttgaagccg
     7201 gcaaaatgtt gtttgccgat ttcgttacct ttcaatagac caaggtgaga gcttaattgc
     7261 cattgacctt ttgtaacacc cacgcccact tcagttttca gataacgtcc gaaacgttgg
     7321 ctaaaggtat tgtcattgac tgccacactc aagcgtttgc tgctggcatc gacataatgt
     7381 gcggcaaggc taggctcaac tttccagctg gagaggtcaa aggccttgct gactttcaca
     7441 cctgcttggt aggccataaa gtgtgctttc gggctgcgga cttctacttc acccagtttg
     7501 taattttgtg cgctgaagcg atagtaacgt gtaccaacag caggctgaac ttgtacgccg
     7561 gctaagtcga agttatgtcc aagatttgcc ccaatcgcca taatatgacg gttaaagcgg
     7621 ttctccccga acaggtcgat acggtttttc gctttaccgt agctaccgtc taggctgacg
     7681 aaagtgccgt tttccgaatg ccatttacca tataggcttg ccatcagtaa gttggattta
     7741 ccgttcactc cctcatcaaa ttccgcatta gcgtggttgc gggatagcat cacaccggca
     7801 ttgataccgt ttgcaagcgg taattctatg cctaactgag tgaggttggt ttgttgctca
     7861 tacgggcgat ataaatccga ttggtgttcg gttttttgat gttcgatact gctccaaacg
     7921 tggaatttat cgtgttttgc gaacagttgg cgatctaagt cttgcccgat ttgcaacgca
     7981 gaatttgcct gtgccgatag ctcggaaaga gcggtattgg catattggct tacccagtcg
     8041 ctttgttgaa tattactact gtcagtgtct gcgtgtgctt gtagatgctc acaatttgca
     8101 ccattctctt ggcaaagttt tagtgcctca gcgtttaaaa ttgcttcact atttccagca
     8161 gttatacgta aggtagctaa ttttgcttct acttgttttg caatattttg tgcagtttca
     8221 accgcttgtt ctacgttagc aatttgcgca gcggatgctt tagcattgtt ttctgctgta
     8281 gtgagtgcag cactcgcttg tgtgactttc tgttttgcta ccgtaatttg cccttggatt
     8341 tggcggtaat acgttggata gtaacggcgg tagtaatcag catattgttg caaacgggtt
     8401 aattcgctat ttgcttggct aagctgagat tgtgcgtttg cagcggcttg tttagcattt
     8461 ttctctgctt ggcgctcttt ctcggcttct gcgttcaaac ggctgatttc ctgtttttgt
     8521 ttatccgctt ctgcaatagc atggtctaat tccgctttcg ccgttgcaat cgactgattc
     8581 cgatctttag catcttttaa cggattgtat aagcgatagc cgttgcttcg atttgccagc
     8641 acataacgat atgcacctaa atcaacataa ccgttttcca gtgaaacttt agctttatca
     8701 gcctgagcct gatgggttaa ggttaataaa cttaacgggc taaccacttc agcttctttg
     8761 ccactgttgc gtaatgcaag agtgaaagtc ccctcggcga gaccatttac ttgtaggtga
     8821 tcgcctttgc cagcagcaat atcggttaag tagttgattt tggcattacc ttggaaatta
     8881 ccgtcaataa tcaacgtatt aaaacgagtc ggtatctcgc ctgttacaaa tttttcattc
     8941 aggttgatgg ttgaaccgtt ttctgccact aaattgccag tacggctatc cccagtgttt
     9001 gtccagcttg cttggttagc taaacgaatg ctcgttgttg ctgctgcttg aatggtgcca
     9061 atcaggttcg ctttgccgat gtgtaattgg ctctgttctg ccaagctgac atcaccatta
     9121 atttgtgttg tgggcagtgt tgcaaaaatt tcattagaaa cgaccgcttg tgtatcacag
     9181 cttgttttac cagtgtactc agaatagtag caacttggag tttgctcgtt gataaagcct
     9241 aaatgtagct gagcatggtc attcgctgac aagtttgctt gcaggtttga gacattacga
     9301 ccaacataaa gctgagcgta ttggtttagc tgcatattat cagccttaaa ttgacgattt
     9361 tgccaatcat cggcataaac tacatcttgc ttattgatcg catcataagc gtgaggggtc
     9421 ggtctgccgg aaagtagcac tttacctcct ttcacagtta gatcgccgtt taagttcaaa
     9481 ccaccagaga aggtataaat actattgagc ttttcttgct ctattggtgc gtttttacgg
     9541 gctaatactt tgttgatcgc ttgattgcga tctgcagcta aaaattccca atgctcgctg
     9601 cttaagccac cgcgaggaac aggttgtttt ggatcacctt ttaatgcaaa gtattcccga
     9661 attttcgttt tcggattggt gaacagataa atatctgcac cagctactag ccccttaccc
     9721 caagttactt cagaggcagt tgccggtggt gtagaggctt ttggatcata caccacattc
     9781 aaacgaccgt tatgctgggt gttatctgtt tcgccaataa aaccattaaa gctattgtag
     9841 cgatgctcca gacctttttg cgcgagtact tgttttactg ctgcatcctt gttagaagac
     9901 aggaaggtcc aatgggcatt acttgcgcca ttcgttggca tatattggtt tggattgcct
     9961 ttgagcgtaa agtaatcggt acggttatta gcatgtgggt tgatatattc ataaatatca
    10021 gcactgtttt ctttccacgt tccccatttt aagtccgttg cttcaggggg cttcgggcca
    10081 ttaatggtga gtgttgcagc tttttgtagg tgattattaa caatacgcgc accatcatca
    10141 ctattttgaa tacgattaaa cgcaatgttg ttgccgttga gatctaaacg accaccacgg
    10201 aagccaaagt aaatcttatt tggatcgact tgatcagcac tgtttaatac aacggttgaa
    10261 cggccgctca caataccaac ttggttaaat gcagattttt tgccattttc acccgcttgt
    10321 tgggtcaaaa tgacagtgcc ttgtccaaca ctaatgtcac caaggttttc gcctttaccg
    10381 ttaatatgta gtgtcccttc gcccaattta gataaacggt caccttgtgg gtttttgact
    10441 tgccaattaa cttgtttacc ttttgccact acaattccag ctcctaccca gctttgatca
    10501 ttttcagggc gaactgttgc attagcattg aaatttaatg cgccagcacc ttggtcaata
    10561 tcggttttta atacgatagt gctatcttgt ccttcaatgt ttagggtttt tccggtgtta
    10621 tcttgcggaa gttgtagctg aggtgtgcca ccatagccta atggagaagc gctattgtct
    10681 ttaattgaac gatcagcaag cgaaaccgtt aagtttttcc ctttattacc aatgctactg
    10741 tctttaccct gtgcagacca ttcaaaaacg gcattttgtt gcgcattggt taatgtgccg
    10801 gcaatatctt cggcaaattg tttatcgtga taatcctgac ggataatacc ggcacggttg
    10861 tagtagttgt ttgcaggcgt accaaaggtg taagtagcca gtagtagcca gcgtttcgtt
    10921 ttctgatcgt agccatacac acctgaaccg ctatcaccac gagtaccaaa tgcagttaat
    10981 gctccatatg ggctattgtt taatgcattt cctctaaaat ctagccagta atcggaatta
    11041 tgtaccgcga ttggtaaatg aactcccccg gttaaatatt ggtaagatgt tgaaatttgg
    11101 ttgtctttat gctcggtatt tttgactatt tgacgaccag agcctaagcg agcaaagtga
    11161 gtaaaacgac tactatcttg gtaggctgaa gcattattac ccagatcagt cactgttgca
    11221 ggtgcgacct cagtgactaa tttattgaga cgtggcactt gataatcttg atgttgccca
    11281 ccttcaacaa ttccatagtc gttacgatca acgactttgt aagtgtagtg gtgtgcatcg
    11341 gcattagtgc ctggggcgcc aaattccaca gtattgtatt gtgtattatg ggcaacgctg
    11401 actaaatatt gcggtgcaac tagagttgct atccctaagt tacggtttgc agcactaaaa
    11461 tctggcatcg gtacatttgg gaagagtgca tctaatgtat tgccactttt atctttaatt
    11521 tcaatattgc ttgcacctac ggtgaattta cccttattct ccgcaaaatc acggtagtat
    11581 tgaagatcga tatcatctct gaaaacagag gctgttgcag ataaactaac agcaaatgcc
    11641 acaaagctgg caaggtaaga aagtggaaag tttttattta tcataaaatg ttaataaatt
    11701 gctaatatta atggagtaaa aaggtggggt attctataga taattattta tttggcaaga
    11761 ggtcttacct gttttttata caaatttaca atcttaagat aggcaaaagc agctacggaa
    11821 tacgcagctg ctttgttgaa agttttattg agtgataagc aggagcatta ttttgcaaat
    11881 tcttgcataa attcgcccgc ttgtttgacc attgaggttg aaccgacaaa gaacggtaca
    11941 cgctggtgta gctctgttgg cttaatatct aaaatcggat taataccatc tgtcgccatt
    12001 cctccggctt gttcggcaag aaacgcaatc gggttgcatt catagagtaa acgcaatttc
    12061 ccttttggat atacggtgga ggttgggtaa atataaatac cgccttttaa taaattgcgg
    12121 tggaaatcgg acaccagcga gccgatatag cgtgaagagt atggacgttt gatggcttca
    12181 tcggtttctt ggcaatactt aataaagcgt ttcacacctt gtgggaaagt aacatactgc
    12241 ccttcattga tggagtaata ttttccttcg gtaggggttt ttagatcagg gtgtgaaaga
    12301 ataaataagc ctaatgacgg atcataggta aaaccgttca cgccattgcc tgtggtgtaa
    12361 accagcatgg ttgaagaacc ataagtaaca taaccggcgg caacttgttt acgcccttcc
    12421 tgcaagaaat cttcttgtgt tactggggtg ccgatagggg agacacggcg gtaaatagag
    12481 aagattgttc ccacagaaac attcacttca atattggagg agccatcaag cggatcggtc
    12541 attaaaatat attttgcatt tctcccacgc tcattatcaa aggcaacaaa attatcatct
    12601 tcttccgatg caaagccggc aacatcttca cgtgcaagta acgctttttt catcgtttcg
    12661 ttggcgaata catcaagttt catttgggct tcaccctgta cattttcaga acctgcagta
    12721 cctaaaatat cttgacttaa accggcacgg ttgatttctc gatgaataat tttagcggct
    12781 aaacgaatag aagataaaat cccacttaat tccccttttg cccctgggta ttctgcttgt
    12841 ttttcaataa taaattcgcc gagtgttttc atttttttct ccttcgctat gttagaatgc
    12901 ccttattata acgattacct ttaaagaggt agcggtttaa gctttaattc tgtgatccac
    12961 ttcacatttt atctttcaag atgaggagca atacgatgaa taatctacga atcaaaatga
    13021 tcagccctga aatagcatta gcaaaatata atgaattgga tgttttagag attaatcact
    13081 ccaaagtgaa ggctaaaatt gcattgcaag gtgcgcagtt actgagttgg caacctaaaa
    13141 atactcagca agatgtatta tggttaagtg aaattgaacc tttccaaaca ggtgtggcga
    13201 ttcgtggcgg cattccgatt tgccaccctt ggtttggtac agacaaaaag cctgctcacg
    13261 gcacagcccg tattcaagcg tggaatttgc tggaacatac ttcctcagca gatgggattc
    13321 gtgttgtttt ggggttagaa aatcaggcaa aactcgagat gatactaggc gaagtctgtg
    13381 agttgtattt tactcactta gcaccggagc cggcacaggc tgcattgcat acttattttc
    13441 aggtcggtga tattacgaaa acggaagtac aaggcttagc aacacaatgt tttgataaat
    13501 tgacaaatca agaaattgaa gtgccatcac ctcgcaagat aagtgaaaat gtggattgta
    13561 tttatccggc acaagcggtg aattttattc aagattttgc aaatcagcgc accattaaag
    13621 ttgagcatat taatgctacc gaaaccgtac tttggaatcc ttggcataat accgttagtg
    13681 cgatgacaga aacggcgtat caaaatatgg tatgtgtgga aacagcacgg ataaatacct
    13741 tacttcaaca aaatgaaacg ctaggagtga gaattttggt gcgataatgt ttaatgagag
    13801 caagcagctg aattagtaat attttttaca aatttaaccg cttgtctctt gtatttacac
    13861 tagatcaaac agtaaaaatt ccacgtcatc ttgagtgttt atttcaagtg ctttctcttg
    13921 acgaatgcct aaggcatcgc tggtggaaag ttcaacgcca ttgatgttta gattcccttt
    13981 caccacttgt agccagtaac tgcggtttgg ctctaatgcc agttgctctg atttggctgt
    14041 tgggtattga taacgccaca gcttcatatc ttggtaaatt ttaaatgaac cttcttctgc
    14101 gtttggcgaa agaatcaagg ttgctccctc tttcggggca aatctatctt ggttgtagcg
    14161 aggggtcaca tctttttggt ttgggataat ccaaatctgg taaaggtgta aggtttcatt
    14221 cggatctgga ttaatttctg aatggaaaac cccggttccg gcagacataa tttgaaattc
    14281 gccagctttt acttcggttt tattacccat actgtcttcg tgagccacac gtccttttaa
    14341 catataagtt aaaatttcca tattatcgtg cgggtgcttg ccaaaaccgt gattaggtgc
    14401 aatgaaatct tcgttaatta cccgtaaatg tgaaaaatgg atatgtttta ggtcgtaata
    14461 attggcaaag gaaaaagtgt ggtagctttt aagccagcta aaagaaccat cgcctcgttc
    14521 attggctttt ctcagttgta acattatcta ttccttatgt aagtgttaag ttgtgccatt
    14581 ttactttatg tcaaaaatag ataaaccct
//
LOCUS       DANLXS010000052        14566 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.052, whole genome shotgun sequence.
ACCESSION   DANLXS010000052 DANLXS010000000
VERSION     DANLXS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14566
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.052"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..228
                     /locus_tag="PXD71_001130"
     CDS             <1..228
                     /locus_tag="PXD71_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL6291263.1"
                     /translation="YDASVQQGWVIRSCAATNKTMVWEDGKEYPLYTLDTSSASHPVY
                     TGKRREVNTEGRASQFKDRFKGFASTLSNQK"
     gene            237..362
                     /gene="ykgO"
                     /locus_tag="PXD71_001131"
     CDS             237..362
                     /gene="ykgO"
                     /locus_tag="PXD71_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDL6291264.1"
                     /translation="MKILNSLKTAKSRHPDCQIVRRKGKLYVICKTNPRFKARQR"
     gene            542..1315
                     /locus_tag="PXD71_001132"
     CDS             542..1315
                     /locus_tag="PXD71_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6291265.1"
                     /translation="MLKKLSIAALALTLSVSAFAKESLLERINNKGTITVGTEGTYAP
                     YTYHDASGKLTGYDVEVTRAVAEKLDVKVEFKETQWDAMLAGLKGERFDLVANQVALT
                     SPERRATFDKSESYSWDGAALLVREDETKIKTPADVKGVKTAQTLSSNYGEIARENGA
                     DIVPIDGMAQGLLLIQQKRADATFNSHLSLLDYLKKNPNSGLKIVWEKEEKVGAGLIA
                     NKGNDEALAKISKAIVELREDGTLKKLGEQFFGKDISVQ"
     gene            1398..2114
                     /locus_tag="PXD71_001133"
     CDS             1398..2114
                     /locus_tag="PXD71_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL6291266.1"
                     /translation="MLNDLLLSIPFMTQARVDLVLSGFWPMVQAAFLVSIPLAIASFI
                     IGMLIAVAVALVRISPATGFFHRLLLWLVKGYISIIRGTPMLVQICIVFYGLPAIGIF
                     IDPIPAAIIGFSLNIGAYGSETIRAAISSVPKGQWEAGYTIGMTYMQTFRRIIAPQAF
                     RVAVPPLSNTFIGLFKDTSLASVVTVTEMFRVAQQVANMSYDFLPVYIEAGLIYWCFC
                     WVLFLIQARLEVRFNRFVAK"
     gene            2124..2876
                     /locus_tag="PXD71_001134"
     CDS             2124..2876
                     /locus_tag="PXD71_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL6291267.1"
                     /translation="MLKIRNIHKTFGNNAILRGIDLDIQKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEQGTLEFSGNAPLKIDFAVKQSKKEILALRRKSGMVFQNYNLFPHKTAL
                     ENVMEGPVFVQQQAVEIAQKNAKALLEKVGLADKAELYPFQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNTMKELANEGWTMVVVTHEIKFALDVADVVVVMDG
                     GEIVEQGSPQQLFNHPQHERTKRFLNQIRADK"
     gene            complement(3092..3625)
                     /locus_tag="PXD71_001135"
     CDS             complement(3092..3625)
                     /locus_tag="PXD71_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL6291268.1"
                     /translation="MEFLAPVISLIVGFLSFVLVLRTWLQFCRVDPRLPISQTLLKIT
                     EPMVSPVGKLIPTVKNICFPALLIAAALVTLQFILFGVDLPRAVLIGLLSVVKTFGQI
                     LFFTTLIRALMSWVTQGNHPLDYTVAQITEPVLGFIRKLLPRTGMLDFSVMVLGFGLL
                     LLNSLFYQIFGGLWAAA"
     gene            complement(3731..4072)
                     /locus_tag="PXD71_001136"
     CDS             complement(3731..4072)
                     /locus_tag="PXD71_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDL6291269.1"
                     /translation="MAIQRNARQRKKMHLGEYQELGFLIKFTFAEGTGIDQIDETVDR
                     FIDEVIRPNGLALEANGYLNWEGLVCLEKLGKCDESHRQLVKTWLETNGLKEVQVSEL
                     FDIWWDYPAQA"
     gene            complement(4096..4845)
                     /gene="trmB"
                     /locus_tag="PXD71_001137"
     CDS             complement(4096..4845)
                     /gene="trmB"
                     /locus_tag="PXD71_001137"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL6291270.1"
                     /translation="MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQ
                     RDMMNNNWANLGLDYQPMPFNFEQIFGNNNPVVLEIGFGMGKSLVEMAEQNPNRNYIG
                     IEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDSIADGTLGGLQLYFPDPWQKAK
                     HHKRRIVQPEFISRVIRKLSPNGFIHFATDWENYAEHMLEVLRQFESELQNTSATNDF
                     IPRPNFRPLTKFEERGHRLGHGVWDLYFTKK"
     gene            complement(4855..5805)
                     /locus_tag="PXD71_001138"
     CDS             complement(4855..5805)
                     /locus_tag="PXD71_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL6291271.1"
                     /translation="MNSRILATGSYLPTKVRTNADLEKMVDTSDEWIVTRSGIRERRI
                     AEPDETVATMGAKAAQQALEMAGIEANDIDLIVVGTTTNSHAYPSAACQIQGLLQIED
                     AIAFDVAAACTGFVYALTVADQFVRNGQVKKALVIGSDINSRMLDETDRSTVVLFGDG
                     AGAVILEASEELGIISTHLHASPDLENNLTLRSQKRGEERSGFIEMQGNATFKIAVNQ
                     LSSVVEETLEANNLQKSDLDWLIPHQANLRIITATAKKLEMEMDQVVVTLDKYANTSG
                     ATVPVALDEAVRDGRIQRGQLLLLEAFGGGWTWGSVLVKF"
     gene            complement(5951..6970)
                     /gene="hemB"
                     /locus_tag="PXD71_001139"
     CDS             complement(5951..6970)
                     /gene="hemB"
                     /locus_tag="PXD71_001139"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDL6291272.1"
                     /translation="MQVIQSQYPLRRMRRLRKHDFSRRLVAENQLTVNDLIYPVFVIE
                     GENQRVQVPSMPGVERLTIDQLLVEAAELVKYGVPAVALFPVVGDVKKSLMAEEAYNP
                     QGLAQRAVRALKAQFPELGVITDVALDPFTTHGQDGIIDETGYVLNDVTTEVLVKQAL
                     SHAEAGADIVAPSDMMDGRIGKIREALEQNGFINTQIMAYSAKYASNYYGPFRDAVGS
                     AGNLKGGNKYTYQVDPANGNEGLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKENFGV
                     PTFAYQVSGEYAMHMAAIQNGWLKERECIMEGLLCFKRAGADGILTYFAKRVAKWLYE
                     EAQAK"
     gene            complement(7051..7806)
                     /gene="tatC"
                     /locus_tag="PXD71_001140"
     CDS             complement(7051..7806)
                     /gene="tatC"
                     /locus_tag="PXD71_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDL6291273.1"
                     /translation="MSQVDESQPLITHLIELRNRLLRSFICVLLVFCGLVYWANDIYT
                     LLATPLTDNLPDGATMIATNVATPFFTPIKLTIVVSVFLSVPFILYQIWAFVAPALYK
                     QEKRLVYPLLVSSTLLFYCGVAFAYYVVFPLVFGFLTSTAPEGVTMATDISSYLDFVL
                     TIFMAFGICFEVPVAIILLCWAGVTTPEDLKAKRPYIIVAAFVIGMLLTPPDIFSQTL
                     LAIPMCLLFEVGVLFARFYRPKDDDEPSEQVAQ"
     gene            complement(7793..8437)
                     /gene="tatB"
                     /locus_tag="PXD71_001141"
     CDS             complement(7793..8437)
                     /gene="tatB"
                     /locus_tag="PXD71_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDL6291274.1"
                     /translation="MFDIGFSELVLIMIVGLVVLGPKRMPIAIRTVMGWVSTIRGLAA
                     NVQNELAQELKLQELKESIKKAEELNLSSLSPELNKTVEDLKMSATQLQDSLNSAKNE
                     LEKTAAESSTIPKLTDEQVAEIQQKIEQESQVLEAQELQKNAQNKPLEVDSLSEQLNV
                     TRTEKPLSADELAEQAEMDESLLTLESYYPPDDDLATPQSSESAQKSEANNVAS"
     gene            complement(8441..8662)
                     /gene="tatA"
                     /locus_tag="PXD71_001142"
     CDS             complement(8441..8662)
                     /gene="tatA"
                     /locus_tag="PXD71_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDL6291275.1"
                     /translation="MGGISIWQLLIVVAIIVLLFGTKKLRTLGSDLGESVKGFKKAMA
                     DEKKDAEFEKVEAKDAISAEQKAKEKEQA"
     gene            complement(8773..10410)
                     /gene="ubiB"
                     /locus_tag="PXD71_001143"
     CDS             complement(8773..10410)
                     /gene="ubiB"
                     /locus_tag="PXD71_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDL6291276.1"
                     /translation="MNIKNLNRFYHIIRTFLRYGIDEAIPNLPITRKIKLGRKALFWV
                     KNQHSDKPYGVRLRLALQELGPVWIKLGQMLATRRDLFAPELADQLALLQDNVEPFDG
                     KIARQIIEQALGGKLETWFENFDETALASASIAQVHTAKFNQNQPLAGKDVVLKVIRP
                     GIEPVIKADLELMYEIAHFIPKLSNDGKRLRAVEVVREYEKTILDELDLRKEMHNAIR
                     LRANFENSEMLYVPEMYQDFCHKNLIAMERIYGIPVSNIAELEANSTDMKLLAERGVQ
                     VFFTQVFRDSFFHADMHAGNIFVNREHPENPQYIGIDCGIVGTLNHNDKRYLAESFVA
                     FFNRDYRRVALMHVESGWTPPDTDIDKFEQAFREVCEPIFAKPLSEISFGHVLLNLFN
                     VAREFNMEVQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAMV
                     RDIKQRLPQYREHFAEFPEALFKALQEQKQINFRLAEINESLKAQNSNANSRFLVLSI
                     SAIVICGAFWKFESLPIWLSVPLLTLFPLVLILSQRK"
     gene            complement(10484..11461)
                     /locus_tag="PXD71_001144"
     CDS             complement(10484..11461)
                     /locus_tag="PXD71_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL6291277.1"
                     /translation="MKQIIAQARENAVRAVDFQRVLMYWHIGKRIFEQEQQGQERADY
                     GTYLIKMLAKELEPEFGSGFCSRQLERYRQFYRAFPIATALRSQLNWTQYRRLIQISD
                     SDKREFYLAESCKNNWSSRQLERQINSSLYERLLLSNDKESVLAVARNEQQPVDPKQL
                     IKDPMVLEFLGLKRESAYYEKDLEQAIITHLQDFLLELGNGFSFIARQKRLHLDGDDF
                     FVDLVCYNRLLQCFVIFEIKTHKLTHQDLGQLQMYVNYFDRVEKLPHENATIGVLLCA
                     DKNDAVVKFSLPENQNKIFASQYQLYLPSEQTLIAEVQKEIENFEQKYP"
     gene            complement(11622..12269)
                     /locus_tag="PXD71_001145"
     CDS             complement(11622..12269)
                     /locus_tag="PXD71_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HDL6291278.1"
                     /translation="MLNNVKQQLMLPQFTFGLIETAFNALLKRSPHIEPNLRKLSGKV
                     LKIALTSPKIDFFVLFSESRTDWLGNYEGEADCTVQLAFDTLPKLADKQKLTELINNK
                     SLVLTGDIQVLQHFTALLEQLEKDPAELLSPFVGDVVAQTSTDLAKKLLGKVKAQFEQ
                     NNQHLVENLIQERPVLVHRLQAVHFYDQVEELAQQALKLEQKFTHYVRSSSHQQS"
     gene            complement(12460..13212)
                     /gene="ubiE"
                     /locus_tag="PXD71_001146"
     CDS             complement(12460..13212)
                     /gene="ubiE"
                     /locus_tag="PXD71_001146"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDL6291279.1"
                     /translation="MQQSAETTHFGFKTVAKEEKQQLVANVFHSVAGKYDLMNDLLSF
                     GIHRVWKRFTIDCSGVRKGQKVLDLAGGTGDFTAKFSRIVGESGEVILADINSSMLEV
                     GREKLRNLGVVGNVSYVQANAECLPFADNTFDCIVISFGLRNVTDKDKALRSMFRVLK
                     PGGRLLVLEFSKPIIDPISQLYNFYSFNILPKVGEVVVNDAESYRYLAESIRMHPKQD
                     ELKAMMEQTGFESVNYYNLSAGIVALHRGYKF"
     gene            13557..14510
                     /gene="corA"
                     /locus_tag="PXD71_001147"
     CDS             13557..14510
                     /gene="corA"
                     /locus_tag="PXD71_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDL6291280.1"
                     /translation="MIRAFGLDNACLISVDETNPTLLNDAIWIDLIDPSEAERTVLEN
                     RLDQTLAEERELEDLEASARFFEDEDGLHLHSFFYCLDDDDYADIATVAFTIRDGRLF
                     TLRDRDLPAFRLYRMRSRREKLIDSNAYELLLDLFETKIEQLADVIEEVYADLEKLSR
                     VILNGQQENENFNDALSRLTEFEDASSKVRLCLMDTQRALSFLLRKTRLPNNQLEQAR
                     DIMRDIESLQPHNESLFQKVNFLMQAAMGYINIEQNRIMKFFSVVSVMFLPATLVAST
                     YGMNFEFMPELHFQYGYPMAIGLMIVAASAPYLYFKRKGWL"
BASE COUNT         4147 a         3258 c         2847 g         4314 t
ORIGIN      
        1 tatgatgcct cagtgcaaca aggctgggtt atccgttctt gtgcggcaac caacaaaaca
       61 atggtatggg aagatggcaa agaataccca ctttacacct tagatacctc atcggcgtct
      121 caccctgttt acacaggtaa acgccgtgaa gtgaatacag aaggtcgtgc aagtcaattt
      181 aaagatagat ttaaaggctt tgcttcaacg ctttcaaatc aaaaatagag gataaaatga
      241 aaatcttaaa ctcacttaaa accgcaaaat cacgccaccc ggattgccaa attgtcagac
      301 gcaaaggcaa actttatgtg atttgcaaaa ccaacccgag atttaaagct cgtcagcgtt
      361 aaaaaccagc accttaataa gctaatttat taaggtgttt tttatttcat cttctcaata
      421 ttacctattt taaccacatc aacgatttta ttatttctta ttccatttag ttatttgcaa
      481 gcggtcaatt ttccccttaa atttgcaaaa aatttatttt accaattaaa aggaaatgat
      541 tatgttgaaa aaattatcaa ttgctgcatt agctcttacc ctttctgttt ctgcatttgc
      601 taaagagagc ctacttgagc gtataaacaa caaagggact attactgtgg gtacggaagg
      661 aacttatgcc ccttacacct atcacgatgc gagcggtaaa ttaaccggtt acgatgtaga
      721 agtaacccgt gcggtggctg aaaaattaga cgtaaaagtg gaatttaaag agacacaatg
      781 ggacgctatg ttagccggtt taaaaggtga gcgttttgac ttagtggcaa accaagtggc
      841 tttaaccagc ccggaacgtc gagcgacatt tgataaatcc gaatcttaca gctgggacgg
      901 tgccgcctta ttagtgcgtg aagatgaaac taaaatcaaa acccctgctg atgtaaaagg
      961 cgtgaaaact gcccaaacct tgagctcaaa ctatggcgag attgcccgtg aaaacggtgc
     1021 agacatcgtg ccaattgacg gtatggctca aggcttactg ttaattcaac aaaaacgcgc
     1081 cgatgcaact ttcaatagcc acttatcgct attagattac ttgaaaaaga acccgaattc
     1141 cggcttaaaa attgtgtggg aaaaagaaga aaaagtgggt gcaggcttaa ttgcgaataa
     1201 aggcaatgac gaagcattag cgaaaatcag caaagcaatc gtagaattac gtgaagacgg
     1261 cacgttaaaa aaattgggcg agcagttctt cggtaaagat attagtgtac aataatccat
     1321 attggattag gcttggggcg aattcgcccc ttttattttt aaatctccct caaaacggga
     1381 gattttaggt ttttcagatg ttaaacgatc tacttctctc aatccccttt atgacccaag
     1441 ctcgggtcga tttagtgcta agcggttttt ggccgatggt acaagcggca tttttagtct
     1501 ctattccgct ggcaatcgcc tcatttatta tcggtatgtt aattgccgtt gcagttgcat
     1561 tagtgcgaat tagccctgca acaggctttt ttcaccgctt gctactgtgg ctggtaaaag
     1621 gttatatctc gattattcgc ggcacgccga tgttggttca gatctgtatt gtgttttatg
     1681 gtttgcctgc aatcggcatt tttatcgatc cgattccggc ggcaattatc gggttttcac
     1741 tcaatatcgg ggcttatggc tcggaaacga tccgtgctgc gatctcctcc gtgccaaaag
     1801 gacaatggga agccggttat actatcggta tgacttatat gcaaactttt cgccgtatca
     1861 ttgccccgca agcgtttcga gttgctgttc cgccacttag caacaccttt atcggtctgt
     1921 ttaaagatac ctctcttgcc tccgttgtta ccgtaaccga aatgttccgg gttgctcaac
     1981 aggtagcaaa tatgtcttat gacttcctac ctgtttatat tgaagccggt ttgatttatt
     2041 ggtgcttctg ctgggtgcta tttttaattc aggcgagatt ggaagtccgc tttaaccgat
     2101 ttgtggcgaa ataaggagga attgtgctta aaattcgcaa tattcataaa acctttggca
     2161 acaacgctat tctacgcggc attgatttag atattcaaaa agggcaagtg gtagtaattt
     2221 taggcccttc cggttcaggc aaaaccacct ttcttcgttg cttaaatgca ctggaaatgc
     2281 ccgagcaagg cacattggaa tttagtggta atgctccgct gaaaattgat tttgccgtaa
     2341 aacagagcaa aaaagagatt ttagcgctac gccgcaaatc cggtatggtg ttccaaaact
     2401 acaacctgtt cccgcataaa accgcattgg aaaatgtgat ggaaggaccg gtatttgtgc
     2461 aacaacaagc ggtcgaaatt gctcaaaaaa atgcaaaagc cctgttagaa aaagtcggac
     2521 tggcggataa agcagaactc tacccgtttc aactgtcagg cgggcaacag caacgggttg
     2581 gcattgcacg agcattagca attcagccgg agctgatgtt atttgatgaa ccaacttcgg
     2641 cacttgatcc tgagttagtg caagacgtgc taaatacgat gaaagaactg gcaaatgaag
     2701 gctggacaat ggtcgtggtt actcacgaaa tcaaatttgc ccttgatgtt gccgatgtag
     2761 tggtggtaat ggacggcggt gaaatcgttg aacaaggctc gccacagcaa ctgtttaacc
     2821 acccacaaca cgaacgaact aaacgttttt taaatcaaat cagagcggat aaataagaaa
     2881 cgatagcgct agcgtccacg ctagtgctat ctaataaatg gtacaagcgg ttaattatgt
     2941 ataaattgtt atactgtttt ggcacgagcg tggacgctcg cgccatcgta cagtaaaatc
     3001 agctaacaag gttccccata aaggataaat aagacaaggc tccttaatct tttagattaa
     3061 ggagcttatt tttagatttt agaaatccga tttaagccgc agcccatagt ccaccaaaaa
     3121 tttggtagaa caagctattt agtagcaata agccgaaacc tagcaccatt accgagaaat
     3181 ctaacattcc cgttcttggc aggagcttgc ggataaaacc taacaccggt tcggtaattt
     3241 gtgctaccgt gtagtctaat gggtggttgc cttgagtgac ccagctcatt aatgcacgaa
     3301 tcaaggtggt gaaaaataga atttgtccga aggttttgac cacacttaat aagccgatta
     3361 atactgctct gggtaaatct acgccaaaca gaataaactg taacgttacc aaagcggcgg
     3421 caattaatag tgccggaaag caaatatttt taacggttgg aatcagcttg ccaaccggtg
     3481 aaaccatcgg ttcggtaatt ttaagtaagg tttgtgaaat cggtaaacga ggatcaaccc
     3541 gacaaaattg cagccaagtt cttagcacca gcacaaaact taaaaagcca acaataagcg
     3601 aaataacagg agctaaaaat tccatctctt tttctctttc tatacaaaac aagcggtcgt
     3661 tttttagaaa aaatttgcaa attttcagta aaatctgacc gcttgtatct tattaaatta
     3721 actaggcaaa ttaagcttgt gctgggtagt cccaccaaat atcaaataat tcgctcactt
     3781 gaacctcttt taagccgtta gtttctaacc aagttttcac taattggcgg tgagattcat
     3841 cacatttgcc taatttttct aagcagacta aaccttccca gtttaagtag ccgttcgctt
     3901 caagggctaa accgtttggt cgaatcactt catcgataaa acgatctacg gtttcatcaa
     3961 tttggtcaat gcctgtgcct tcagcaaagg tgaatttaat taagaaacct aattcttggt
     4021 attcacctaa gtgcattttt ttacgttggc gtgcgttacg ttgaatagcc attttttctc
     4081 tcctctatga ataaatcatt tctttgtaaa atataaatcc cacaccccgt gtcctaaacg
     4141 atgccctcgc tcttcaaatt tggttagcgg gcggaaattc ggacgtggaa taaaatcatt
     4201 agttgctgat gtattttgca actcgctctc aaattggcgt aatacttcaa gcatatgttc
     4261 tgcgtaattt tcccagtcgg tggcaaaatg aataaagccg ttagggctta attttctgat
     4321 cacacggctg ataaattcag gctgcacaat acggcgtttg tggtgttttg ctttttgcca
     4381 agggtctggg aagtaaagct gtaagccacc taatgtaccg tcagcaatgc tgtctcgcaa
     4441 aatttcggtg gcatcgtggc aaattactct taaattcttc acgccttttt ctaacgcata
     4501 agcgatacaa gcccccaccc cgggcgtgtg cacctcaatg ccgatatagt tgcgattcgg
     4561 gttttgctct gccatttcca ccagcgattt tcccattccg aagccgattt ctaacactac
     4621 cgggttgtta ttgccgaaaa tctgctcaaa attaaagggc attggctggt aatctaagcc
     4681 taaatttgcc cagttgttgt tcatcatatc acgttgataa tcgctcaaac gaccggtacg
     4741 caatacaaaa ctccgcactt ttcgtaaata acgcccatct tcggtaaact cggcttgctc
     4801 aacggtttta cgttttttat ctgcaaaagt ctgtttttct gacattttct tgccttaaaa
     4861 cttcactaat accgaacccc aagtccagcc accgccgaat gcttcaagga gtagtaattg
     4921 cccacgttga attcgcccgt cacgcaccgc ttcatctaat gccaccggca ctgttgctcc
     4981 actggtattg gcatatttat caagggtaac caccacttga tccatttcca tctctaattt
     5041 tttcgccgtt gcggtgataa tgcgtaaatt cgcttggtgc ggaatcagcc aatctaaatc
     5101 ggatttttgt agattattcg cttctaaggt ttcttctacc acgcttgaaa gttggtttac
     5161 tgcaatttta aaggtggcat tgccctgcat ttcaataaag ccggaacgct cttcgccacg
     5221 cttttggctt cttaaggtta agttattttc taaatccggt gaggcgtgca agtgggttga
     5281 aataataccc agctcttcac tcgcctctaa aatcaccgcc ccagctccgt caccaaataa
     5341 tactactgtg ctgcggtcgg tttcgtctaa cattctggaa ttgatgtctg aaccaataac
     5401 caatgccttt ttcacttgcc cgttacgcac gaattgatcg gcaacagtta aagcatacac
     5461 aaatcctgta caagccgctg ccacatcaaa agcaatcgcg tcttcaatct gaagcaagcc
     5521 ttgaatttgg caagctgcac ttgggtaagc gtgggagttg gtagttgttc ccaccacaat
     5581 taaatcaatg tcatttgcct caattcctgc catttccagt gcttgttgag cggcttttgc
     5641 ccccattgtt gccactgttt cgtcaggctc ggcaatacgg cgttcacgaa tgccggaacg
     5701 ggttacgatc cattcatcag aggtatcgac cattttttct aaatcagcat tggtacgcac
     5761 ctttgtcggt aaatagcttc ccgttgctaa aattctacta ttcatatttt tattattctc
     5821 aatcaattaa atgataatcc gttattatag cttgaaaacc aacaaatata taggctttac
     5881 aagcggtcat ttttagcaaa aaagttgcat tcacgaacga ttttgcacta gaaccaccgc
     5941 ttgctagtat ttatttcgct tgagcctctt cgtatagcca cttcgctact cgcttggcga
     6001 aataagttag aataccgtct gctcctgccc gtttaaagca gagtaatccc tccataatgc
     6061 actcacgctc tttcagccag ccgttttgga ttgccgccat atgcatcgcg tattcgcccg
     6121 aaacttggta ggcgaaggtt ggcacgccaa aattctcttt cacacgccac accatatcca
     6181 aataaggcat acccggtttc accatcacca tatctgcccc ttcttgaata tccatcgcca
     6241 cttcgtgcaa gccttcgttg ccgttagccg gatcgacttg gtaagtgtat ttattaccgc
     6301 cttttaagtt gccggcagaa cctactgcat cacggaacgg tccgtaataa ttagacgcat
     6361 atttagccga atacgccata atttgcgtat tgataaaacc gttttgctca agggcttcac
     6421 gaattttgcc gattcgaccg tccatcatat cgctcggggc aacaatatcc gcccccgctt
     6481 ctgcgtgcga taacgcctgt tttaccaata cttcagtagt cacatcattc agtacatagc
     6541 ctgtttcatc aataatgccg tcttgtccgt gcgtggtaaa cgggtctaac gccacatcag
     6601 taatcacgcc taattccgga aattgagctt ttaaagctcg cacggcacgc tgtgccaacc
     6661 cttgcgggtt ataggcctct tctgccatca gtgatttttt cacatcaccc accacaggga
     6721 aaagagccac cgcaggcacg ccatatttaa ccagttctgc ggcttcaact aaaagctggt
     6781 cgatagttaa acgctctaca cccggcatag aaggcacttg tactcgctga ttttcccctt
     6841 cgatgacaaa aacagggtaa atcaaatcat taacggtaag ttgattttct gccactaagc
     6901 ggcggctgaa atcgtgcttg cgtaaacgac gcatacggcg taaaggatat tgggattgaa
     6961 ttacttgcat ttttaatttc ctaattctta aaatacctat tcttgaaata agaaaggcat
     7021 agcaaacagg tgctatgcct tttcaaaata ttactgtgca acctgctctg atggttcatc
     7081 atcatctttg gggcgataaa accgagcaaa tagcacacca acttcaaaca ataaacacat
     7141 tggaattgcc aataatgttt gcgagaaaat atctggtggg gtgagcaaca taccaattac
     7201 aaatgctgcc acaataatat aagggcgttt tgcctttaaa tcttcgggtg tggttacgcc
     7261 tgcccaacat aacaagataa tcgcaaccgg cacttcaaag cagatcccga atgccataaa
     7321 aatggttaaa acgaaatcaa gatagctact aatatccgtt gccattgtta ccccttccgg
     7381 agcggtagag gttaaaaagc caaatacaag cggaaataca acataatagg caaaagcgac
     7441 accacaataa aacagcaatg tgctagatac caataatggg taaaccaaac gcttttcctg
     7501 cttgtataaa gcaggggcga caaacgccca aatttggtac aaaataaaag gaactgataa
     7561 aaacaccgaa accacaatgg ttaatttaat tggggtaaaa aacggagtgg ctacattggt
     7621 tgcaatcatt gttgcaccgt ctggtaaatt gtccgttaaa ggtgttgcca ataaggtgta
     7681 aatatcattg gcccagtaaa ccaatccgca aaacaccaag agcacacaga taaaactacg
     7741 cagtaatcgg tttctcagct caattaaatg tgtaatcaga ggttgagact catcaacttg
     7801 cgacattatt tgcctccgat ttctgcgctg attctgatga ttgaggcgta gctaaatcat
     7861 catctggcgg atagtaagat tccaacgtta aaagcgattc gtccatttct gcttgttcag
     7921 ccagctcatc agccgaaagt ggcttttctg tccgcgtaac attaagttgc tcagataaac
     7981 tatccacttc aagcggttta ttttgcgcat ttttttgcaa ttcttgcgcc tctaacacct
     8041 ggctttcttg ctcgattttt tgctgaattt ctgcgacttg ctcatcggtt aatttaggaa
     8101 ttgtgctgct ttccgcagcc gttttttcca actcattttt tgcagaatta agcgaatctt
     8161 gcaactgggt ggctgacatt ttcagatctt ccaccgtttt atttaattct ggcgaaaggg
     8221 agcttaaatt caactcttct gcctttttaa tgctctcttt taattcttgc agtttcagct
     8281 cttgagccag ttcattttgt acgtttgcgg ctaaacctcg aattgtactc acccagccca
     8341 ttacggtacg aatcgcaatc ggcatacgtt tcgggcctag caccaccaag cccacaatca
     8401 taatcagtac taattcagaa aaacctatat caaacacgga ttaagcctgc tctttctctt
     8461 ttgctttctg ctctgctgaa attgcatctt ttgcttccac tttttcaaat tcagcatctt
     8521 ttttctcatc tgccattgct tttttgaagc cttttacaga ttcgcccaaa tccgaaccta
     8581 aagtacgtaa tttcttcgta ccgaaaagta atacgatgat tgcaaccaca atcagaagct
     8641 gccaaatact aataccgccc ataaaaactc ctttcattta atagggaata gataaaataa
     8701 aacgctgttt attatatgtt actttgacat ataaaaaagt aattttgcaa aaaatttgtc
     8761 aaaaacaacc gcttatttgc gttgactcaa aatcaatacc agtggaaata aagttaataa
     8821 cggcacactt agccatatcg gcaaagattc aaatttccaa aatgcgccac agatcacaat
     8881 cgctgaaatt gacagcacaa gaaaacggct atttgcgttt gaattctgtg cttttaaact
     8941 ttcgttaatt tccgctaaac ggaaattaat ttgcttttgc tcttgtaagg ctttaaacaa
     9001 tgcctccgga aactccgcaa aatgctcccg atattgcggt aaacgttgct taatgtctcg
     9061 aaccatcgct ttcacaccta cctgctcatt cagccagttt tgtaaaaacg gttttgcggt
     9121 ttgccataaa tccaactgtg gataaacctg cctgcccaaa ccttcaatat aaagaagtgt
     9181 tttttgcaac aacaccaact gcggctgcac ttccatatta aattctcttg ccacattaaa
     9241 caggttgagc aaaacgtgtc cgaatgaaat ttccgataac ggcttagcaa aaatcggctc
     9301 acatacctca cgaaaggctt gctcaaattt atcaatatcg gtatccggcg gcgtccagcc
     9361 cgattccacg tgcattaagg caacacggcg gtaatctcgg ttgaaaaatg cgacaaaact
     9421 ttcggctaaa tagcgtttgt cattgtggtt caacgtgccg acaatcccgc agtcaatgcc
     9481 gatatattgc ggattttccg ggtgttctct gttcacaaaa atattgccgg cgtgcatatc
     9541 ggcgtggaaa aaactgtcac ggaatacttg ggtaaaaaat acctgcacac cacgctctgc
     9601 cagcaatttc atatcggtgc tgttcgcttc caactcggca atgttagaca ctggaatgcc
     9661 ataaatccgc tccatcgcaa tcaagttttt atggcaaaaa tcttgataca tttcaggcac
     9721 atacagcatt tcgctatttt caaaattcgc ccgaagccga atggcattgt gcatttcttt
     9781 gcgtaaatcc agctcgtcta aaatagtttt ttcgtactct cgcaccactt caaccgcccg
     9841 tagccgtttg ccgtcattcg ataatttcgg gataaagtga gcaatttcat acatcaactc
     9901 taaatccgcc ttaatcaccg gctcaatgcc cgggcgaatc acttttaaca ccacatcttt
     9961 gcctgcaagc ggttgatttt ggttaaattt tgccgtgtga acctgtgcaa tcgaagcaga
    10021 agctaaggca gtttcatcaa agttttcaaa ccaagtttca agctttccgc ccaaagcctg
    10081 ctcgataatt tgacgagcga ttttgccgtc aaacggttca acattatctt gtagcaaggc
    10141 gagctggtcg gcaagctccg gtgcaaacag atcacgtctg gtagctaaca tttgcccgag
    10201 cttgatccaa accggtccaa gctcttgcaa agccaaacgt aaacgtacac cgtaaggttt
    10261 gtctgaatgt tgattcttca cccaaaacaa agccttacgc cctagcttta ttttgcgggt
    10321 aattggcaga ttcggaatgg cttcatcaat accatagcga agaaaagtgc ggataatatg
    10381 gtaaaagcgg ttgagatttt tgatgttcat tgaaatatat atttaatggt aattaggaag
    10441 attttagcaa aattctgtaa aaatttaacc gcttgttggg ctactatggg tatttctgct
    10501 caaaattctc aatttctttc tgtacttccg caataagtgt ttgttcagag gggaggtaaa
    10561 gttggtattg gctggcaaaa attttattct gattttccgg tagcgaaaat tttaccaccg
    10621 catcattttt atcggcacaa agcaagacac caatagtggc attttcgtgc ggcagttttt
    10681 caactcggtc aaaataattg acgtacatct gtaattgccc caaatcttga tgggtgagct
    10741 tgtgggtttt aatttcaaaa atcacaaaac attgaagtaa acggttgtaa caaaccaaat
    10801 cgacaaaaaa atcatcaccg tctaggtgca aacgcttttg gcgtgcgata aacgagaaac
    10861 cgttacccaa ttctagtaga aaatcctgca aatgcgtaat gatggcttgc tctaaatcct
    10921 tttcgtaata agcactttcc cgttttagtc cgagaaattc caataccatc gggtctttaa
    10981 tgagctgttt cggatcaacc ggctgctgct catttctggc aacagcaagc acactttcct
    11041 tatcattact gagcaataat cgctcgtata gactactatt aatttgccgt tccagctggc
    11101 gtgaactcca attattctta caactttcag ccaaataaaa ttcccttttg tcagaatcgg
    11161 agatttgaat tagacgacga tattgtgtcc aattcaattg cgaccgcagt gcggtcgcaa
    11221 ttggaaacgc acgataaaac tggcgataac gctctaattg acggctacaa aaaccacttc
    11281 caaattctgg ctcaagctct tttgcgagca ttttaatcag gtatgttcca taatcagcac
    11341 gctcttgccc ttgttgctcc tgttcaaaaa tccgttttcc gatgtgccaa tacatcagca
    11401 cacgttggaa atctaccgca cgaaccgcat tttcacgagc ttgagcaata atttgtttaa
    11461 tatcttagat catcgttgtt tctattaaca taaaagcctc cgttttggat taagtttatg
    11521 cttaatctat ccggaggcaa tttctttcac tcaacttttc gagtcatatc acaattttat
    11581 tttgttaaat ttaattttgc acaaacgctt gcgtttgaac atcagctttg ctgatggctc
    11641 gaagagcgaa cgtagtgagt aaatttttgc tctaatttga gcgcttgttg ggcaagttct
    11701 tcgacttggt catagaaatg caccgcctgt aaacgatgca ccaacaccgg tctttcttgg
    11761 attaaattct caaccaaatg ttgattattt tgctcaaatt gagcttttac tttacctagc
    11821 aattttttag caagatcggt gctagtttga gccactacat cgccaacaaa aggggaaagc
    11881 agttcggcag ggtctttttc gagctgttca agtaatgcag tgaaatgttg caacacttga
    11941 atatcgcccg tcagcaccaa cgatttatta ttaatcagct cggttaattt ttgcttatcc
    12001 gctagtttcg gcagggtgtc aaatgccagt tgcaccgtac aatctgcttc cccctcgtag
    12061 ttgccaagcc aatcggttcg gctttcgcta aacaacacaa aaaagtcgat tttaggcgaa
    12121 gttagggcga tttttagcac ttttccggac aatttacgca aattcggctc aatatgaggc
    12181 gagcgtttaa gtaacgcatt aaaagcggtt tcaatcagac caaaggtaaa ttgcgggagc
    12241 attaattgtt gttttacatt atttaacatt cgtacctact taccctcttc acttttggga
    12301 gaaggataga ttgttcttca ttaagaagca aaatcaggga gaggggtatt tgcaaaatat
    12361 tgaataaata tgaccgcttg ccccctctct ctgcttattt cgctaaacgc tcataagcgg
    12421 tctctctccc acaaagggag agagtcattt aagagaaaac taaaatttat aacctctgtg
    12481 taatgccaca atgccggcac ttaagttgta gtaattcacg ctttcaaagc cagtttgttc
    12541 catcattgct tttagctcgt cttgtttcgg gtgcattcgg atcgattcag ctaaatagcg
    12601 gtaactttcg gcgtcattta ccaccacttc gcccactttc ggcaaaatat tgaatgagta
    12661 gaaattataa agctggctga ttgggtcgat aatcggtttt gagaattcca acacaagtaa
    12721 gcgaccgccc ggttttagca cgcggaacat tgaacgtagg gctttgtctt tgtcggtaac
    12781 attacgcaag ccaaaactaa tcacaataca atcaaaagtg ttatcagcaa aaggcaagca
    12841 ttcggcattc gcttgaacgt agctcacatt tccaaccacg cctaaattgc gtaatttttc
    12901 ccgcccgact tccaacattg agctgttaat atcggctaaa atcacctcac cactttcacc
    12961 aacaatgcga gagaattttg cggtaaaatc acccgtgccg ccggcaagat ccagcacttt
    13021 ttgcccttta cgcacgccac tgcaatcaat ggtaaagcgt ttccacactc gatgaatgcc
    13081 gaaagaaagc aaatcgttca ttaggtcata tttgcctgcc accgagtgaa acacatttgc
    13141 gaccagttgc tgtttttctt ctttcgctac ggttttaaac ccaaagtggg tggtttctgc
    13201 cgattgttgc acttcttgtt gcacttcatc tttaggtgct aattgttcta catcgctcat
    13261 tttcttgtct ctagttgcaa aatttaggcg taaccatagc aaaaaagcgg tcgtttttct
    13321 ttaacttttt gcaaattttc ttaagtaaat aaccgctttc ttgactagaa aagccaaatt
    13381 ctgtaacatt cgcacactat tttttcttga ggattacaca ttttggacag tcatagtcga
    13441 tacttacccg tttaacctat cttgtctaac ctcaatgtta ccgacaaggt cggtaatgct
    13501 cgcctagtat ctttgttcct ttttgttcca agcctcctaa ctatttggag ctaaacatga
    13561 tccgtgcttt tggactagat aacgcatgtt taatcagcgt tgatgaaact aacccaactt
    13621 tattaaatga tgcgatttgg attgatctca tcgatccatc agaagctgaa cgaaccgtat
    13681 tagaaaaccg cttagaccaa acactcgctg aagaacgtga attggaagac ttagaggcgt
    13741 ctgcccgttt ctttgaagat gaagacggtt tgcacctgca ctcgttcttt tactgcttag
    13801 atgatgacga ttacgccgat attgccactg tcgccttcac tattcgtgac gggcgtttgt
    13861 ttaccttgcg tgatcgggat ttacccgcat ttcgcttgta tcgaatgcgt tcccgccgtg
    13921 agaaattgat tgacagcaac gcctatgaac tcttactcga tttatttgag accaaaatcg
    13981 agcagcttgc agacgtaatt gaagaagtct atgctgactt agaaaaatta agtcgggtta
    14041 ttctaaacgg tcagcaagaa aatgagaatt tcaatgatgc cctttctcgc ttaactgaat
    14101 ttgaagatgc aagttcaaaa gtgcgtttat gtttgatgga tacgcaacgg gcgttaagtt
    14161 tcttactgcg taaaacccgt ttgccgaata atcagctaga acaagcccgt gacattatgc
    14221 gagatattga atctctgcaa ccgcacaatg aatccttgtt ccaaaaagtg aattttttaa
    14281 tgcaagcggc aatgggttat atcaatattg agcagaaccg aattatgaaa ttcttctcgg
    14341 tggtgtcagt gatgttccta cctgctacgc tggttgcttc tacttacggt atgaactttg
    14401 aatttatgcc ggaacttcat ttccaatatg gctaccctat ggcgattggt ttaatgattg
    14461 ttgccgcctc cgccccttat ctttatttta aacgtaaagg ttggttataa tttattttta
    14521 aagcctatag ttgaataact taggctttct tattttaatg aatatg
//
LOCUS       DANLXS010000053        14482 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.053, whole genome shotgun sequence.
ACCESSION   DANLXS010000053 DANLXS010000000
VERSION     DANLXS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.053"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            67..1512
                     /gene="aldA"
                     /locus_tag="PXD71_001148"
     CDS             67..1512
                     /gene="aldA"
                     /locus_tag="PXD71_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDL6291281.1"
                     /translation="MVKQHKMYINGEFVPALDGRTLAVYNPATEKAISEVPRGGADDA
                     KIAIDAAEAAQEAWERLPAIERGKYLRQIAVGIRERADEIARTISEEMGKTLSLATVE
                     VHFTADYLDYMAEWARRYEGEIIQSDRPNEHIFLYKKAIGVTTGILPWNFPFFLIARK
                     AAPALVTGNTIVIKPSEDAPNNAILFSEIVHQVGLPKGVFNVVTGYGNEVGPELSGNE
                     KIGMVSFTGSQFAGQKVMEAASKNIIKVNLELGGKAPAIVLDDADLELAATAVVNSRI
                     INSGQVCNCAERIYVQSSIKEQFVQKLVEKMQAVKYGNPLEQQDIDMGPMVNEKGLHK
                     VQEMVTHALHQGGNLVLGGNVVEGTGYYFEPTIIDNVDNSMDIMRHEIFGPVIPVATF
                     DTIDEAIRLANDCEYGLTSSVYTQNINKAMKVISRLKFGETYVNRENFEAMQGYHAGW
                     RKSGIGGADGKHGLEEYLQTQVVYLQFDDKV"
     gene            1768..2415
                     /locus_tag="PXD71_001149"
     CDS             1768..2415
                     /locus_tag="PXD71_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HDL6291282.1"
                     /translation="MSIFQQTNPNRRRYGLAVFIGIIAGIISAFVKWGAEHPFPPRSP
                     IDLFTAACPQPVLDAMNAAADQVAAKTIALQECSRAFLNPPHVFLRDYIGIDPTEAAF
                     TFADHAFNWIGVTHMIFSLVFAIGYCIVAEIFPKIKFWQGIGAGIIANICVHYITFPA
                     LGLTPPVAEWPLYEHISELVGHIFWFWTIEVIRRDLRNRITREPDAEVPLDQPFR"
     gene            complement(2548..3924)
                     /gene="trkA"
                     /locus_tag="PXD71_001150"
     CDS             complement(2548..3924)
                     /gene="trkA"
                     /locus_tag="PXD71_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDL6291283.1"
                     /translation="MKVIILGAGQVGSTLAENLVSEDNDIVLVDNDPTRLDKLKDKHD
                     LQVIRGEYASPQTLRDAGAGDADLLVAVTNSDEVNMIACQIAYTLFNVPTKIARIRSA
                     DYVRERERLFNDDVLPIDHIIAPEILVKEDILRLIHYPGALQITHFADELVSIVNVKA
                     YYGGPLVGYPISALRDHLPHIDARVVAIFRQDKAIVPQGSTIIEAGDEVFFICATQHI
                     RAVMSELQRLDRPHKRIMIVGGGSIGSSLARDLEDQYRVKIIERNPVRAEKLAEKLSK
                     TMVLTGDASDEELLFEEHIENIDLFLAVTSDDEANIMSALLAKRLGAKKVIILVQRPA
                     YLHLIQGGTIDIAISPQQATISALASYVRKGDILQVSSLKLGVAGAVEIIAHGDATTS
                     KVVGRQIRELKLPQGAIVGAVVRGEEVIIAHKSTMIAENDRVIIFVNDKKQIDEIEKL
                     FQLGVFFL"
     gene            complement(3924..5255)
                     /gene="rsmB"
                     /locus_tag="PXD71_001151"
     CDS             complement(3924..5255)
                     /gene="rsmB"
                     /locus_tag="PXD71_001151"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL6291284.1"
                     /translation="MKKQSFKALSSRAISATIILQVLDQGKSLSTLIPDAQKQLEPKD
                     LPLVQEITFGVCRVLPRLESIIKLLVEKPLKGKTRLVHCLLLVGLYQLLYMRVPAHAA
                     VDEVVNATKTLKLDSFRALTNGVLRRFLREQEEILAKVDKHWQTLHPEWLVNKLKKAY
                     PNWREIVEANNQRPPMWIRVNQQHIKTNDYAALISELVEPEFAKNSENPTACVPKCAL
                     LLERAVNVNQLPHFEQGWATVQDAHAQWSAELLGAENGETVLDACAAPGGKTTHILEK
                     APKAKVMALDIEESRLSRVRENLVRLGQIAQVICGDASKPEEWLEEGVLFDRILLDAP
                     CSATGVIRRHPDIKWLRKEGDIAELATLQGEILKALWQRLKPNGILLYATCSVLPQEN
                     SEQIQRFLQDTPNAKQLEIDFNGEKVLEKQFFPQPNGGDGFFYAKLQKVAE"
     gene            complement(5307..5732)
                     /locus_tag="PXD71_001152"
     CDS             complement(5307..5732)
                     /locus_tag="PXD71_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL6291285.1"
                     /translation="MYNKLLIAIDLADLKSAKYVVDTALQMTAKNPNAVYRVVSIIEP
                     VDNSLISAFLPKNFDKEVVAEANQKLHEFTKNHFPEGSKVQHIVAYGTIYEEICRIAD
                     EKSVDLIMMLASSKPNAKGLSSNTVKVARNTTKPILVLR"
     gene            complement(5825..8473)
                     /locus_tag="PXD71_001153"
     CDS             complement(5825..8473)
                     /locus_tag="PXD71_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HDL6291286.1"
                     /translation="MSVESKVDYDDLQDMVASNDSGGRTPSGLAKKAIVYVAILWSVF
                     QIYYASPLPFVFQEWLTHAGINLNVVVDDTKARSIHLAFAMFLAFLSYPAFASSPKHH
                     IPKIDWVFAIVGAGLALYYIFFYEGLVKRFGAPNTQDIIAGCLGILLVLEATRRSLGL
                     PLVIIAIVFLIYNFFGQYFPADWIVSHRSGSLSQIINQQWITTEGVFGVALGVSTKYV
                     FLFVLFGALLDKAGAGNYFIKTAFAYLGHLRGGPAKAAVVSSALTGLISGSSIANVVT
                     TGTFTIPMMKRVGFTKEKAGAVEVASSVNGQIMPPVMGAAAFLMIEYVNMPYSQLITH
                     AFLPALISYIALVYIVHLEACKMNLQGLPRAEEPSPILVFLLRTVASILVIVGLAIGV
                     YYGLGWIRDVAEDYAFMIVCAFLGLWYLLAIRRVASYPDLEADDPNSEIVSLPKRKPT
                     VNAGLHYLIPVVVLLWCLMVEMLSPGLSAFWGTITLIFIQLTQRPLLNFFRKESVTKE
                     IIKQGVRDVVDSLEAGGRNMTGIALATATAGIIVGVVALTGFGVQLSSVIELLSMGNI
                     VLMLILVAVFSLILGMGLPTTANYIVVSSLMATVIMEVGRQNGLIVPLIAVHLFVFYF
                     GIMADVTPPVGLASFAAAAISGGSPINTGMVAFKYSLRTVILPFLFIFNTDLLLIDLR
                     YPGHGWVVFITATVGVLAFTSATMQYIVTKNKWWETALLVVAAFALFRPGFFMDRIYP
                     SERHIEPVQFEEQLLASKVGQNVTLKVEGLNPYGKEIEFYAQLPVPEGATGEERLKNL
                     GLTLIQTDEKIKVDGVETPKVVIDMVEMDSPAAKAGLNWDQTIHYIAVPQDAPSKDWI
                     FIPALLLVFGIVANQRRRVKKQCVIA"
     gene            complement(8675..9637)
                     /locus_tag="PXD71_001154"
     CDS             complement(8675..9637)
                     /locus_tag="PXD71_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HDL6291287.1"
                     /translation="MKKLVKLSFLASLVMGTASVQAAETFVTIGTGGQTGVYYVVGQS
                     ICQLVNRDSAKTGLKCNAPSTGASVANLNAIAAKEMDMGIAQSDWQYHAYNGSSSFEG
                     KKNDKIRAIFSLHPEPFTVMARTDSGIKSFDDLKAKRVNVGDPGSGTRATMNVILAAK
                     GWTDKDFKVASELKPAEMASVMCDNNLDAITYNVGHPNGALKEAAASCDAKLVPVTGP
                     EIDKLVADHSYYAKATIPGGLYKGSDEPTETFGVYATLVTSADVDADKVYNVTKAVFD
                     NFDRFKRLHPAFEHLKQEEMIKNALSAPLHEGAIRYYKEKGWIK"
     gene            complement(9821..11668)
                     /gene="selB"
                     /locus_tag="PXD71_001155"
     CDS             complement(9821..11668)
                     /gene="selB"
                     /locus_tag="PXD71_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDL6291288.1"
                     /translation="MIICTAGHVDHGKTSLLQALTGTNTAHLPEEQKRGLTIDLGYAY
                     LPIENDILGFIDVPGHQRFLSNMLAGLGGIQHALLVISAEEGIKPQTEEHLDILHLLN
                     FQHIMIVITKADRAEETQISHLIEQVKTSYPFLAEAKTFVTSAKTQQGIEELKAHLIQ
                     LNQQHTQTHKPFRYAIDRVFNVKGAGLVVTGTTVAGKVSVGSELFLSNGKKVRVKAIH
                     AQNTPSETGVAGQRLALNIANVEKEEIKRGDWITELEPKFATDRITVQLTANQAFKEN
                     SIVHLYHFASHITGKLNLLEGQQAVKNQQFFAEVILDEPLHIAVGDKLIIRSGDDSLT
                     LAGAEVLEIHSPKRHKRTEARLALVKNLAKTTACNDFATRLDLYLQNRAMDLSLLLWA
                     EQCFAEDIAKLGFDVSSKWLFNSDFKAQAQQRVLEKMAEYHLQHQDQIGVTKARLYRI
                     ALLDLPENLANQFIEDLVEQKALVNSRGWLHLPEHRIELNADELQIWQQIRPLFEATN
                     QALWVRDIASQLSIDETQMRNLLYKVGKLGYLIPIVKDRFLLSEQIGQFATLIKDFIA
                     QNGSISVNQLRDELQYGRKLTVQLIEYFDRSGFLRRKGDVHLLRDSETF"
     gene            complement(11678..11974)
                     /locus_tag="PXD71_001156"
     CDS             complement(11678..11974)
                     /locus_tag="PXD71_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6291289.1"
                     /translation="MMKFDYNIVWLQKAEIKLFYQADYILQQSQNPIIAEQFYDAIKS
                     EVDKLSFTADVYRLRKKKEIPILNGKYQVKFLIGQENVYIVDFKSARQNSYSFL"
     gene            complement(11971..12147)
                     /locus_tag="PXD71_001157"
     CDS             complement(11971..12147)
                     /locus_tag="PXD71_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291290.1"
                     /translation="MELILAQDLAKLRTDKPQSFYTNDELDLLHQLENGLIETKSHAQ
                     VMEDLRKKLGITEK"
     gene            complement(12216..12848)
                     /gene="pdxH"
                     /locus_tag="PXD71_001158"
     CDS             complement(12216..12848)
                     /gene="pdxH"
                     /locus_tag="PXD71_001158"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDL6291291.1"
                     /translation="MDLHHIREDYTKKVLSIRDCHSNPITQFEQWLSEAINAKVNEPT
                     AMNIATVAANGRPSSRMVLLKEVNSQGFVFFTNYQSRKGWEIADNPFGCLTFFWAELQ
                     RQVRIEGKIAKIPEAESDAYFDSRPYTSRLGAWASEQSQPIENYKALLLKSAQFALKY
                     PLSVPRPPHWGGYILIPDSIELWQGRSSRLHDRIHYWLENNEWKKERLSP"
     gene            complement(12906..14288)
                     /locus_tag="PXD71_001159"
     CDS             complement(12906..14288)
                     /locus_tag="PXD71_001159"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDL6291292.1"
                     /translation="MQQLFRQIPSLDLLLKKPQAVELCQKFAYSSVAEQARSLLTQAR
                     QFISQQQALPDFIADENLFFIRLEQKVQALSEVKIRSVFNLTGTVLHTNLGRGLWSEK
                     AIQAATNAMRNNVALEFDIEAGKRSHRDNYISELLQILTGAEAACIVNNNAAAVLLML
                     ATFAQGKEVIVSRGELVEIGGAFRIPDIMAQAGCKLVEVGTTNRTHLKDYRNAITENT
                     AFLMKVHTSNYHIEGFTSSVSEEELVALGKEFNLPVITDLGSGSLTDMQALNLPAEPK
                     VQQKVAAGVDLVSFSGDKLLGGPQAGIIVGSQAMISQLQQHPLKRVLRCDKVILSALE
                     ATLRHYLFPEQLTDNLPTLHLLTQPLETLQHKAERLKQALHKRLNSAYILQIELSLAQ
                     IGSGALPTETLPSVAVTISAEKQSDLIALEQQFKTYPSPIIGRFAQQKFWLDLRSVAE
                     FDKLIEMLEY"
     gene            14356..14450
                     /locus_tag="PXD71_001160"
     tRNA            14356..14450
                     /locus_tag="PXD71_001160"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14390..14392,aa:Sec,seq:tca)
BASE COUNT         4119 a         3264 c         2907 g         4192 t
ORIGIN      
        1 gcaaaggcgg ttcagttacc gaatgatagt catcaacttc cactgtggat aaaaaaggag
       61 caactcatgg tgaaacaaca taaaatgtat attaatggtg aatttgtgcc tgcgttagat
      121 ggtcgcactt tagcagtcta taaccctgcc accgaaaaag ctatctccga agtccctcgt
      181 ggcggagctg atgatgccaa aatcgctatc gatgctgctg aagccgccca agaagcttgg
      241 gaacgtttac cagcgattga acgaggaaaa tatttacgtc aaattgctgt ggggattcgt
      301 gagcgtgccg atgaaattgc tcgcactatt tccgaagaaa tgggtaaaac tctgtcctta
      361 gcaaccgttg aggtacattt taccgctgac tacttagact atatggcaga atgggcgaga
      421 cgttatgaag gggaaattat ccaaagtgat cgcccaaatg aacatatttt cctatacaaa
      481 aaagcgattg gcgtcaccac cggcatttta ccttggaact tcccgttctt cctcattgcc
      541 cgtaaagcgg ctccggcatt agtaacaggt aatactattg tgattaaacc gagtgaagat
      601 gcacctaata atgcgattct cttttcggaa atcgtgcatc aagtcggttt acctaagggg
      661 gtatttaatg tcgtcaccgg ctatggcaat gaagtcgggc cggaactttc aggcaatgaa
      721 aaaatcggta tggtttcctt taccggtagc cagtttgccg gacaaaaagt gatggaagcc
      781 gccagcaaga atatcattaa agtcaatttg gaattgggcg gaaaagcacc ggctatcgta
      841 ttagacgatg cagatcttga gcttgctgct accgctgtag taaattctcg aatcatcaat
      901 tcaggacagg tttgtaactg tgcagaacgc atttatgtac agagttcaat taaagagcaa
      961 tttgtccaaa aattggtaga gaaaatgcaa gcggtgaaat atggtaatcc attagaacaa
     1021 caagacattg atatggggcc gatggtaaat gaaaaaggct tgcataaagt gcaggaaatg
     1081 gtgacacacg cactgcatca aggaggtaat ctggtattag gtggtaacgt ggttgaagga
     1141 acaggctact actttgagcc aactattatc gacaatgtag ataactctat ggatattatg
     1201 cgtcacgaaa tttttggtcc ggtcattccg gttgcgacat ttgataccat tgatgaagcg
     1261 attcgcttag ccaatgattg tgaatatggc ttaacctctt cggtttacac gcaaaatatc
     1321 aataaagcga tgaaagtaat ttcccgctta aaattcggag aaacttatgt aaaccgtgaa
     1381 aactttgaag caatgcaagg ctaccacgcc ggttggcgta aatcgggtat tggcggtgct
     1441 gacggtaagc acggcttaga ggaataccta caaactcagg tggtttatct gcaatttgat
     1501 gataaagtat agattcttct aaaaactccc acttatcaaa ataagcggga gttttctttt
     1561 aggttaacct tgtatcaaaa ttgcaaacaa aaatattcta atgttaagta aatgtttaat
     1621 aattatttat ggttaactct ttttggctaa ttaatttgca atttaaatta attgagtgca
     1681 gaatgccatt agcctttttt gaaatcacat tccataaaat ggcttgatta acacaacaca
     1741 attatcactc aaattaggat tttcattatg agtattttcc aacaaaccaa tccaaaccgc
     1801 cgtcgttatg ggctagctgt atttatcggg attattgccg gtattatttc agcattcgta
     1861 aaatgggggg cagaacatcc tttcccacct cgtagcccta tcgatctgtt taccgctgct
     1921 tgcccgcaac ctgtgttaga tgcaatgaat gccgcagcag accaagtggc tgccaaaacc
     1981 atcgcattac aagaatgttc acgtgcattt ttaaacccac cacacgtttt cttacgtgat
     2041 tatattggta tcgatccaac tgaagcagca tttacctttg ctgaccacgc gttcaactgg
     2101 attggtgtca cccatatgat tttctcgtta gtctttgcga ttggttactg cattgtggcg
     2161 gaaatcttcc caaaaattaa attctggcaa ggtatcggtg ccggtatcat cgctaatatt
     2221 tgtgttcact acatcacctt cccggcatta ggtttaacac caccggttgc tgaatggccg
     2281 ttatatgaac acatttctga attagttggt catattttct ggttctggac cattgaggtg
     2341 attcgtcgcg atttacgtaa ccgtattacg cgtgaacctg atgccgaagt gccgttagat
     2401 caaccgttcc gttaatttaa aattagctcg atttatacaa aagccgcaat cacgttgcgg
     2461 ctttcttatt ttacaagcgg tcaaattttc atgaaaattt accaaacaaa aagcacattg
     2521 tttcatgtag caatgcggag cttggcttta taagaaaaat acaccaagtt ggaacagttt
     2581 ttcaatttca tcgatctgtt tcttatcgtt cacaaaaata atcacacggt cattttctgc
     2641 aatcatggtg gatttgtggg cgataataac ttcttcgcct cgcaccaccg ccccaacaat
     2701 tgccccttgc ggtagtttta attcacggat ttgtcgtcca accacttttg aagtggttgc
     2761 atcgccgtga gcgataattt ctaccgctcc tgcaacccca agtttgagtg aagaaacttg
     2821 taaaatatcg ccttttctaa cgtaactagc gagagcagaa atagtagctt gttgtgggga
     2881 aatggcaata tcaatagtgc caccttgaat caggtgtaaa taggcagggc gttgcactaa
     2941 aataatcact tttttcgccc caagccgttt tgccagcagg gcggacataa tattcgcttc
     3001 atcatcactg gtgacggcaa ggaataaatc aatattctca atatgctcct caaacagcaa
     3061 ctcttcatcg gaggcatcac cggtaagcac catcgtttta gatagttttt ctgctaattt
     3121 ttcggctcga accggattgc gttcgataat ttttacccga tactgatctt ctaaatcccg
     3181 agctaacgaa gagccgatgc tccctccacc gacaatcata atccgcttat gtgggcgatc
     3241 aagccgttgt agttcactca ttactgctcg aatatgttgg gttgcacaga tgaaaaagac
     3301 ttcatcacct gcctcaataa tagttgaacc ttgtggcaca atcgctttgt cttggcggaa
     3361 aattgccacg acccgagcat caatatgcgg taaatgatca cgcagggctg aaatcgggta
     3421 accgaccaaa ggcccaccgt aataggcttt tacattcacg atactgacta actcatcagc
     3481 aaaatgagta atttgcaacg cccccggata gtgaataagg cgtaaaatat cttctttgac
     3541 taaaatttca ggggcaataa tatggtcaat aggcaataca tcatcattaa acaaccgctc
     3601 tcgttctctc acataatcag cactgcgaat acgggcaatt ttggtcggca cattaaataa
     3661 agtgtaagca atttggcaag cgatcatatt cacttcatca ctattagtta cagcaactaa
     3721 tagatcagca tcacctgcac cggcatcacg caaggtttga ggtgaagcat attcgccccg
     3781 aatcacctgt aaatcgtgtt tatctttgag cttgtctaaa cgagttgggt cattatccac
     3841 caagacaata tcattgtctt cactcaccaa attttctgcc agcgtagaac caacttgtcc
     3901 tgcccctaaa ataattactt tcattactct gctacttttt gtagttttgc gtagaagaaa
     3961 ccatcaccac cgtttggttg tgggaagaac tgtttttcta acactttttc gccgttaaaa
     4021 tcgatttcaa gttgttttgc attcggtgta tcttgtaaaa accgttgaat ttgctcgcta
     4081 ttttcttgcg gtaacaccga gcaagtggca tataacaaaa tgccgtttgg ctttaacctt
     4141 tgccataacg ccttgagaat ctcaccctgt aatgtggcta attctgcaat atcaccctct
     4201 ttacgcaacc atttaatatc agggtggcgg cgaatgactc cggttgcgga acaaggggca
     4261 tcaagtaaaa tgcggtcaaa cagcacaccc tcttcaagcc attcctcagg tttggaagca
     4321 tcgccacaaa ttacctgtgc aatctgcccc agtcggacta agttttcacg cactcggctt
     4381 aaacggctct cttcaatatc caacgccata acctttgctt tcggtgcttt ttctaaaata
     4441 tgggtagttt tgccacccgg tgcggcacag gcatctaaaa cggtttcacc attttctgcc
     4501 ccgagcaact ccgctgacca ttgtgcgtga gcatcttgca cggttgccca accttgctcg
     4561 aaatgtggca gttggttcac atttaccgct ctctccagca ataaagcaca ctttggcacg
     4621 caagcggtcg gattttctga attttttgca aattcaggct caaccagctc gctaatcaac
     4681 gccgcataat cattggtctt gatatgttgt tgattgactc gaatccacat tggtgggcgt
     4741 tggttgtttg cttcaacaat ttctcgccag ttcggatagg cttttttaag tttattgacc
     4801 aaccattcag ggtggagcgt ttgccaatgt ttatctacct tcgctaagat ttcttcttgc
     4861 tctcgcagaa aacggcgtag cacaccgtta gtcaaggctc ggaagctatc cagttttagc
     4921 gttttagttg cattcactac ctcatcaacc gcagcgtgtg ccggcactcg catataaagc
     4981 agttggtata aacccactaa cagtaagcaa tgcaccaaac gggttttgcc tttcagcggt
     5041 ttttcaacta ataatttgat gatcgattcc aagcgaggca gcacacggca cactccgaag
     5101 gtgatttctt gcactaaggg taaatctttt ggttcaagct gtttttgagc gtcaggaatc
     5161 aaggtggaaa gtgatttacc ttgatctaac acctgtaaaa taatggttgc acttattgct
     5221 ctagaagaaa gtgctttgaa cgattgtttt ttcataaata aaaatgccat tccgacagaa
     5281 tggcactatt gtaactgaat gcgggattat cttaaaacta aaattggctt ggtggtgttg
     5341 cgagccactt taaccgtatt agagcttaag cccttcgcat ttggtttgct gcttgccagc
     5401 atcataatta aatccaccga cttttcatcg gcaatacggc aaatttcttc ataaatcgta
     5461 ccatacgcca caatgtgctg cacttttgaa ccttcaggaa aatggttttt ggtaaattcg
     5521 tgcaattttt ggtttgcttc agccaccact tctttatcga aatttttcgg caagaatgca
     5581 gaaattaagc tgttatccac cggctcaata atagacacaa cccgataaac cgcattcgga
     5641 ttttttgccg tcatttgtaa tgccgtatct accacatatt tagcactttt taggtcggct
     5701 aaatcaatcg caataagtaa tttgttatac atatttattc cgtaggggca ggtcctgtgc
     5761 ctgcccgaat attaactaaa atagcggacg ggcacaggac ccgtccctac atagcatata
     5821 aatactatgc aataacacac tgttttttca ctctacggcg ttggtttgcc acaataccaa
     5881 ataccaacaa tagagcagga atgaagatcc aatctttaga aggagcatct tgcggtacgg
     5941 caatgtagtg aatggtttga tcccagttta agcctgcttt agctgccgga gaatccattt
     6001 ctaccatatc aatcaccact tttggtgttt ctacgccatc aactttgatt ttttcatcgg
     6061 tttgaattag cgttaagcct aagtttttca aacgctcttc gccggttgct ccttccggta
     6121 caggtaattg agcgtagaac tcaatttctt taccgtaagg gtttaatccc tctactttta
     6181 aagtaacatt ttgacctact ttgcttgcaa gcaactgttc ttcaaactgc accggctcga
     6241 tatgacgttc agacggataa attctgtcca taaagaagcc cggacggaac aaggcgaaag
     6301 cggctacaac cagcaacgcc gtttcccacc atttgttttt agtcacaata tattgcattg
     6361 tagcagaggt aaaggcgaga accccaacag ttgcggtaat aaacacaacc cagccgtgcc
     6421 ccggataacg cagatcaatt aataataaat cggtattaaa aataaataag aacggtaaaa
     6481 tgacggttct taaactatat ttaaatgcca ccataccggt attaatcggg ctaccgcctg
     6541 aaattgctgc cgcagcgaac gaggctaaac caacaggtgg agtcacatct gccataatcc
     6601 caaaatagaa tacgaataaa tgcaccgcaa tcaacggcac gattaacccg ttttgacgac
     6661 cgacttccat gataaccgtt gccattaatg atgacaccac aatgtagttt gcagttgtcg
     6721 gtaagcccat tcccaaaatt aagctgaata cggctaccaa gatcaacatt agcacaatgt
     6781 tgcccattga taggagttca atcacacttg aaagctgaac cccaaagccc gttagtgcca
     6841 caacgccgac aataatccct gctgttgcgg tagcgagggc gataccggtc atattacgac
     6901 cgccagcctc aaggctatct accacatctc gaacaccttg tttgataatc tctttggtta
     6961 cgctctcttt acggaagaaa ttcagtaacg ggcgttgggt taattgaata aaaattaagg
     7021 taatggttcc ccagaatgcg gataagcccg gcgaaagcat ttccaccatt aagcaccaaa
     7081 gtagcacaac cacaggaata aggtaatgta aacctgcatt tacggttggt tttctttttg
     7141 gtagagaaac aatttctgag tttggatcgt ccgcttctaa atcagggtag cttgccacac
     7201 ggcgaatagc cagtagatac cataagccta agaaagcaca gacaatcata aacgcataat
     7261 cttctgccac atcacgaatc caacctaagc cgtagtaaac gccaatcgct aatcctacga
     7321 tcactaaaat tgaagctacg gttcttagca agaagactaa aatcgggctt ggctcttcgg
     7381 ctcttggtaa gccttgtaaa ttcatcttac aagcttctaa atgcacgata tacaccaacg
     7441 caatatacga aattaacgca ggtaagaagg cgtgagtaat cagctggcta tacggcatat
     7501 tcacatattc gatcatcaag aacgctgcag cccccattac cggtggcata atttgaccgt
     7561 ttactgatga ggcaacttcc accgcaccgg ctttttcttt ggtaaagccg acccgtttca
     7621 tcattggaat agtgaatgta cctgtcgtca cgacgtttgc aatcgaagag cctgaaatca
     7681 agccggttaa agcggaagaa accaccgccg cttttgccgg accaccacgc aaatgcccta
     7741 aataggcaaa ggcggtttta ataaagtagt tacctgctcc tgctttatcg agcaacgccc
     7801 caaataatac gaacaggaac acatatttgg tcgaaacccc aagggctaca ccaaacacgc
     7861 cttcagtggt aatccattgt tggttgataa tttgagaaag cgaacctgag cggtggctta
     7921 caatccaatc tgccgggaaa tattgcccga agaagttata gatcaggaat acaatcgcaa
     7981 taatcacaag cggtaagcca agactacggc gggtcgcttc cagcaccaat aaaatcccta
     8041 aacagccggc gataatatct tgcgtatttg gcgctccaaa acgtttcacc aaaccttcat
     8101 agaagaaaat ataatacaac gctaacccag ctcctacgat ggcaaacacc caatctattt
     8161 taggaatatg atgtttaggc gagctggcaa aagcagggta agataggaac gccaagaaca
     8221 ttgcaaaggc taagtggata gaacgggctt tggtgtcgtc caccaccaca ttcaaattaa
     8281 tgcctgcgtg agtaagccat tcttggaata caaacggcaa tggagaagca taataaattt
     8341 ggaacacaga ccaaagaatg gcgacataca caatggcttt tttcgcaaga ccggacgggg
     8401 ttcgcccccc actgtcattg gaggcaacca tatcttgtag gtcatcgtaa tcaaccttag
     8461 attcaactga cataaagact cctcaaaacg caagcggtca ttttcttatt aaattttaca
     8521 aatttcctca aaaaacaacc gcttgctcgt gaaaacactg ttgataagtt atcaattaaa
     8581 ggtgaaaatc gataacttat caacagtttc aaaaaagcaa aaaaccagca cgctaaaaaa
     8641 cgtgctgatt gaatcaaatt acagaaagga aatattactt aatccaacct ttctctttat
     8701 agtaacgaat tgcaccttcg tgtaacggag cagaaagggc gtttttgatc atctcttctt
     8761 gttttaagtg ttcaaacgcc ggatgtaaac gtttgaaacg gtcgaagtta tcaaataccg
     8821 ctttggttac attgtacact ttatctgcat ccacatcggc agaagtcact aacgttgcat
     8881 atacaccgaa ggtttctgtc ggctcatccg aacctttata taaaccgccc ggaatcgtag
     8941 ctttggcata atatgagtga tctgccacta atttatcaat ttcagggccg gttaccggca
     9001 ctaatttcgc atcgcaagaa gcggctgctt cttttaacgc accattcggg tgacctacgt
     9061 tataagtgat ggcatctaag ttgttatcac acattacaga tgccatttct gccggtttca
     9121 attcagaagc cactttgaaa tctttatccg tccagccttt tgccgctaaa atcacgttca
     9181 ttgttgcacg agtaccggaa cccgggtcgc ccacgtttac acgttttgct tttaaatcat
     9241 cgaacgattt aatgcctgaa tcggtacgag ccattactgt aaacggttcc gggtgaagcg
     9301 agaaaatggc acgaatttta tcgtttttct taccttcaaa tgagcttgag ccgttatagg
     9361 cgtggtattg ccagtctgat tgtgcaatcc ccatatccat ttcttttgct gcaatcgcat
     9421 ttaagtttgc aaccgatgcc cctgttgaag gagcgttaca ttttaaacct gtttttgctg
     9481 aatcacggtt tactaattgg caaatagatt gacccacaac ataataaaca ccggtttgac
     9541 cgcctgttcc gatagtcaca aatgtttcag ccgcttgaac agatgctgtt cccatcacaa
     9601 ggcttgcaag gaaagaaagt ttgactaatt ttttcatttt taactcctaa aattaaggat
     9661 cagaaaacaa agatttattg ttttgagcca ctgctcacac tacaagagat acaaaacttc
     9721 tttgagttgc atttgcctct tagagcttgt ttttagcata gattttataa ttagtaaatt
     9781 tattttttcg cataaaaaaa tctatgccta atgccacaag ctaaaatgtt tcactatctc
     9841 ttaacaaatg cacatcgcct tttcgtctta aaaaaccgct acgatcaaaa tattcaatta
     9901 attgtaccgt caattttcgc ccatactgca actcatctcg caactggttc accgaaatgc
     9961 tgccgttttg ggcgataaaa tctttaatta acgttgcaaa ttgcccgatt tgttcgctta
    10021 ataaaaatct atctttgacg attgggatta aatagcccaa tttcccgact ttatacagca
    10081 aattccgcat ttgggtttca tcaatcgaga gctggcttgc aatatccctc acccaaagag
    10141 cttgattggt tgcctcaaac agcggtcgaa tttgttgcca aatttgcaat tcatcggcgt
    10201 tcaattcgat tcgatgttcc ggcaaatgta accaaccacg actattgacc aaggcttttt
    10261 gctccaccaa atcctcaata aattgatttg ccaagttttc aggcaaatcc aataacgcta
    10321 tgcgatataa acgagctttg gtcacgccga tttgatcttg atgttgcagg tggtattctg
    10381 ccattttttc caacacccgt tgctgggctt gagctttaaa atccgaatta aataaccatt
    10441 tagacgagac atcaaacccc agttttgcaa tatcctctgc aaaacattgc tccgcccata
    10501 acagcaagga taaatccatc gctctgtttt gcagatagag atcaaggcgt gtcgcaaagt
    10561 cattacaagc ggtggttttt gctaaatttt ttaccaaagc taaacgggct tcggtacgtt
    10621 tatgccgttt tggtgagtga atttccagca cctccgctcc ggctaacgtt aggctgtcat
    10681 caccgctgcg gatgatcaac ttatcaccaa cggcaatatg aagtggttca tctaaaatca
    10741 cttctgcaaa aaattgctga tttttgaccg cttgttgccc ctcaagcaaa ttcaatttgc
    10801 cggtaatgtg cgaagcaaaa tggtaaagat gcacaatgct gttttctttg aaggcttgat
    10861 tggcagtgag ttgaaccgta atgcgatcgg tggcaaattt cggctcaagc tcggtaatcc
    10921 aatcgcctcg cttgatttcc tctttttcca cgttagcgat atttaacgcc aaacgttgcc
    10981 ctgccactcc cgtttccgaa ggcgtatttt gggcgtgaat cgctttgacc cgcacttttt
    11041 taccattcga caaaaataac tcagagccaa cgctcacttt tcccgccacc gttgtgccgg
    11101 taacgaccaa ccctgctcct ttcacattaa acacacggtc aatcgcataa cggaacggtt
    11161 tatgggtttg ggtatgttgt tgatttaatt gaattaaatg tgcttttaat tcttcaatcc
    11221 cttgttgcgt ttttgccgaa gtaacaaagg tttttgcttc cgctaaaaac ggatagcttg
    11281 ttttcacttg ctcaattaag tggctgattt gagtttcttc cgcccgatct gccttagtaa
    11341 tcacaatcat aatatgctgg aaattcagca ggtggagaat atccaaatgc tcttcggttt
    11401 gtggcttaat cccttcttcg gcagaaatca ccaacagggc gtgttgaatg ccgccaagcc
    11461 ctgccagcat attagataaa aaacgctgat gacccggcac atcaataaac cctaaaatat
    11521 cgttctcaat cggtaaatag gcatagccca aatcaatggt tagcccacgt ttttgttctt
    11581 cgggcaaatg ggcggtgtta gtgccggtta aggcttgtaa aagcgatgtt ttgccgtggt
    11641 caacgtgacc ggcggtgcag atgatcattg atagtcctta taaaaatgag taactattct
    11701 ggcgagcaga tttaaaatct acaatataga cattttcctg accaatcagg aactttactt
    11761 ggtacttacc gtttaaaatg ggaatttctt ttttcttgcg aagtcgataa acatcggcag
    11821 taaaggaaag tttatccact tcacttttta ttgcatcata aaattgttct gcaataatcg
    11881 ggttttggga ttgttgtaat atataatctg cttgataaaa caattttatt tctgctttct
    11941 gtagccaaac aatattgtaa tcaaacttca tcatttttcc gtgattccca attttttgcg
    12001 taaatcttcc atcacctgag cgtgggattt agtttcaatt aagccatttt ctaattgatg
    12061 taataaatca agttcatcat tagtataaaa actttgtggc ttatctgtgc gtaattttgc
    12121 taaatcttgt gcgagtatga gttccatatt tcccccttac aaacttagat tattcaatat
    12181 tgtaacctga taaaactaaa gttcaaggag tgtatttaag gcgataaccg ctcttttttc
    12241 cactcattat tttccaacca ataatgaatg cggtcgtgta gccggctact tctgccttgc
    12301 cagagttcaa tactgtcggg aatcagaata tagccacccc aatgcggtgg gcgaggcact
    12361 gaaagtggat atttcaatgc aaattgagct gattttagca acaaagcctt gtagttttcg
    12421 ataggctgac tctgctcgct cgcccaagcc cccaaacggc tggtgtaagg acgagagtca
    12481 aaataggcat cggattctgc ctctggaatt tttgctattt tcccttcaat ccgcacttga
    12541 cgttgtaatt ctgcccaaaa gaaggttaaa catccaaaag gattatcggc aatttcccac
    12601 cctttacggc tttgataatt ggtaaagaac acaaagcctt gtgaatttac ctctttcaac
    12661 agcaccattc ggctacttgg tcgcccgttt gctgcaacgg tagcaatatt cattgctgtc
    12721 ggctcattca ctttggcatt gattgcctca ctcaaccatt gttcaaattg ggtgattggg
    12781 tttgagtgac aatctcggat tgaaagcact tttttggtgt aatcttctcg aatgtggtgc
    12841 agatccataa ggattccttt atcttattct aaataatcgg acaggcacag tctgccctac
    12901 caaaattaat attccaacat ctcaatcaac ttatcaaact ccgcgaccga gcgtaaatct
    12961 aaccaaaatt tctgctgagc aaaacgcccg ataatcgggc ttgggtaggt tttaaattgt
    13021 tgttccaatg caattaaatc gctctgtttt tcagcggaaa tagtcactgc caccgaaggt
    13081 aaggtttctg tgggtaacgc accgctgccg atttgagcga ggcttagctc aatttgcaaa
    13141 atataagcag aatttaaccg cttgtgtagt gcctgtttta atcgctctgc tttgtgttgc
    13201 aaggtttcaa gcggttgagt gagtaaatga agagtcggca agttatcggt taattgctcc
    13261 ggaaataagt agtggcgtag cgttgcctca agtgccgata aaatcacttt atcgcaacgt
    13321 agcacccgtt ttagcgggtg ttgttgcagt tggctaatca tcgcttggct accgacaata
    13381 atgccggctt gcggcccgcc taataattta tcgccagaga aggaaaccaa atctacccca
    13441 gccgctactt tttgctgaac ctttggctca gcaggcaaat taagagcttg catatcagtt
    13501 agcgaaccac tgcctaagtc ggtaatcaca ggcaggttaa actctttacc gagagccacc
    13561 aattcctctt ccgacaccga actggtgaac ccctcaatgt ggtagttact ggtgtgaact
    13621 ttcattaaaa aggcggtatt ttcggtgatc gcgttgcgat aatcctttaa atgagtgcga
    13681 ttagtcgtac caacttccac caatttgcaa ccggcttgag ccataatatc cggaatgcgg
    13741 aatgccccgc caatttccac cagctcgcca cgggaaacga tgacttcctt gccttgtgcg
    13801 aaggttgcta acatcaacaa caccgctgcc gcattattat tcacaatgca agcggcctcc
    13861 gctccggtaa gtatttgcaa caactcggaa atgtagttat ctcggtgcga gcgcttgcct
    13921 gcttcaatat caaattccaa cgccacattg tttcgcattg cgttggttgc tgcctgtatc
    13981 gccttttccg accacaaccc acgccctaag ttggtgtgta acaccgtacc ggttaagttg
    14041 aacacggaac ggattttcac ctcactcaac gcttgcactt tttgttccag ccttataaaa
    14101 aacaggtttt catcggcaat aaaatccggc aaggcttgct gttgggaaat aaattgacgt
    14161 gcttgagtaa gcaaactcct agcttgttca gccacgctgg aatatgcaaa tttttggcat
    14221 aattcgaccg cttgtggctt tttcaacaag aggtcgagcg agggaatttg gcgaaatagt
    14281 tgttgcataa tgtgaatgat tgagagaata ttttggacaa ttctaataaa taaagtacaa
    14341 tattgccaat ttttcggaag tgcgtcatct ccggtgaggt ggcaggactt caaatcctgt
    14401 tgaggacgcc agcgttctcg ggtgggttca actcccatgc acttccgcca catttcaaaa
    14461 gccgttaatc tttaacggct tt
//
LOCUS       DANLXS010000054        14348 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.054, whole genome shotgun sequence.
ACCESSION   DANLXS010000054 DANLXS010000000
VERSION     DANLXS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14348
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.054"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            25..1143
                     /gene="rnd"
                     /locus_tag="PXD71_001161"
     CDS             25..1143
                     /gene="rnd"
                     /locus_tag="PXD71_001161"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDL6291293.1"
                     /translation="MESLIHYQWVETNEQLEAVCEQARKTKVVALDTEFIRTRTYYPI
                     LGLIQLFDGQQVSLIDPNTISDFSPFVSLLADKNIIKVLHACSEDLEVFEYQFKQLPE
                     PMLDTQVMAAFAGIGISMGFAKLVSHYLNIELDKGASRTDWLARPLSAEQLQYAAADV
                     WYLLPVYEKLAQALTKSTWQSAVAEECVALLKKAKQPINKEKAYKNIANAWRLNREEL
                     AILQVLAKWRIEEAQKRNLALNFVVKEASLVQIAKSCPKHTSSLLEFMHPNEVRIHGK
                     KILWLVEQGKAIESNDYPELIQRLVDEKGYKYYMQAMLQKLLEIQPLDIVPELVASKR
                     QLNQLFKWYIDGKPSEKIPELLIGWRKPFGEQLLSVLS"
     gene            1200..1682
                     /gene="lrp"
                     /locus_tag="PXD71_001162"
     CDS             1200..1682
                     /gene="lrp"
                     /locus_tag="PXD71_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDL6291294.1"
                     /translation="MENKKPPKALDAIDLKILNELQRNGKISNIELSKRVGLSPTPCL
                     ERVKRLEKNNVIMGYKALLNPELLDAPLLVIVEITLVRGKPDVFEEFNKAIQQLDEIQ
                     ECHLVSGDFDYLLKTRVADMVSYRKLLGTTLLRLPGVNDTRTYVVMEEVKQTNYLLLK
                     "
     gene            1693..4353
                     /locus_tag="PXD71_001163"
     CDS             1693..4353
                     /locus_tag="PXD71_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HDL6291295.1"
                     /translation="MIEQLKPHLKGKENLIKLMLLLTGFLGVYLLIAWASYSPLDNAW
                     SVASSITQTTLNKTGHFGAWSIDMLYAMFGKVAVLVPIALIIFSIYSLGMGLSVDMKW
                     KTVFIRLASFLLLMVGLAGLFSVLFSNSAYYLSGGFVGGVWETILSDTIGQFGALLIA
                     MLFTIAGLYFCSVQSLLPILTQFYEWIMAKSEESKTAESLQHLQNTEQKQPLVTENEA
                     VEVVDDHKPQFTDISAFKRPNISGLRHSENVEIDNREMYEIERDLELPTINILIQPDM
                     VNQVAEQATSGYELPNEESNVLQMPTIRLNTAIENEVDTIENSSSLEDLKIAESMLPQ
                     IQLDNDIEDTEEAIDDVREITPEFMVTPPKAMKDVSEQPAYPKGYGDTLIHPLLQRKV
                     ITEKPTTPLPTLDLLDKAPIQSQQITEQEIRETSVRLEAELANFGVKATVEDVLVGPV
                     VTRYEIQPAAGVKASKITNLASDIARGLMFKAIRITEVIPNKPYMGIETPNKHRETVW
                     LRDVLDSDEFRNTTATLPMALGKDISGEPVVVDMAKMPHLLVAGQTGGGKSVGVNTMI
                     LSLLFKLTPEQVRFIMIDPKVVELSIYNDIPHLLTPVVTDMKKAANALRWAVEEMERR
                     YLLVSHLQVRNIEGYNAKIDQAAAMNLPIPDPTWRPGDSMDSLPPPLQKLSYIVLIVD
                     EFADLMMSAGKEVEEYIMRIAQKARAVGIHLILATQRPSTDVITGVIKANIPSRIAFT
                     VASQIDSRTILDSGGAEALLGRGDMLYSGAGSPDIIRIHGAFMKDEEVQRVADNWRAR
                     GKPNYLESIVESRSEEADGKNDGGTGDLDPLFDEVVEYITETGSVSISNIQRRFSLGF
                     NRAARIVDQMEAQGIVSEPLKGGKREVLAR"
     gene            4505..5344
                     /locus_tag="PXD71_001164"
     CDS             4505..5344
                     /locus_tag="PXD71_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDL6291296.1"
                     /translation="MRLDDIKESKNVEDRRFNNVGRTKVGRGKGGILTFIIVLVGAYY
                     GVDLTGLMGGGVEYEEQTSALNESEEKQLRTLSAKVLTTTENIWTSYFQQNGLTYKEP
                     SLVLYRGAIQTACGTGQSAMGPFYCPVDQKVYLDLSFYDDMRKKLRASGDFAFSYVIA
                     HEVGHHVQNLLGITSKTQRAQMKASSKAEANQISVNVELQADCFAGVWGHQLAKANRL
                     EEGDVEEAFNAAQAVGDDRLQQQSRGYVVPDSFTHGSSAQRLAWFRKGLTTGNPAVCN
                     TFN"
     gene            5433..6515
                     /gene="aroG"
                     /locus_tag="PXD71_001165"
     CDS             5433..6515
                     /gene="aroG"
                     /locus_tag="PXD71_001165"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDL6291297.1"
                     /translation="MSYIKNDDVRITSIEELLPPIALLEAYPASDVAAETVAKTRQSI
                     HKILHGADDRLLVVIGPCSIHDPKAALEYAQKIKAMRADPKINQNLEVVMRVYFEKPR
                     TTVGWKGLINDPYLNETFALNDGLRIARKVLSDINDLSVPTAGEFLDMITPQYVADFM
                     SWGAIGARTTESQVHRELASGLSCAVGFKNGTNGSVKIALDAISSAKSPHHFLSVTKF
                     GHSAIVSTAGNPDCHIILRGGDSATNYDADSVAAACAAIEKSGGRPHVMIDFSHANSQ
                     KQFKRQMDVCADVCNQIAGGSDFISGVMIESHLVEGRQDLIEGKGLEALVYGQSITDG
                     CIGWEDSEKALFALAEAVEKRRNFAK"
     gene            6602..7294
                     /gene="tsaB"
                     /locus_tag="PXD71_001166"
     CDS             6602..7294
                     /gene="tsaB"
                     /locus_tag="PXD71_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL6291298.1"
                     /translation="MKPSILALDTATEACSVALQYNGNITFLDEISPRSHTQRILPMV
                     DELLNQANIELKEVNYLVFGRGPGSFTGVRVGVSVAQGLAMGANLPVVAVSNLKAMAE
                     EAYQKLGATKVIALIDARMNEVYFAQFERKGEEWNEVVAEQVCSPEVAISQFQMSEET
                     VVVGTGWAAYSQFSEQNLPLVISDITLPSSRYMLSIAETEIQKGNIQSAFEIEPVYLR
                     NEVTWEKLPHKQ"
     gene            complement(7311..8120)
                     /locus_tag="PXD71_001167"
     CDS             complement(7311..8120)
                     /locus_tag="PXD71_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL6291299.1"
                     /translation="MTMTVIIILIICGIIVNITSALFGIGGGVLMMPILHSLFPELPL
                     QVISACSLTIVIGSATINLCLFHHQQIKINRKNVLIWSLAMMLGVQIGFELSFLFSDN
                     SIIAIFILTLLTLSFKTLFHKPRVKAIVPPKPKEAVSNATMCSLGGLIAGITGIGGGS
                     IMAPIVTQIRSVSPKQVAVYTNYMMVIGGIGNLYGYLSRPFVLNSTLATWQIGYVNFA
                     IVGTVVISSLFTVYFSMKLRNILSPELTTKLLGVILFTIACYMGFLQYLFT"
     gene            8219..8770
                     /locus_tag="PXD71_001168"
     CDS             8219..8770
                     /locus_tag="PXD71_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL6291300.1"
                     /translation="MNNMPTPEQLMSCQLSVMSDLEKGVVIYSQGYHAREFYFLKSGL
                     IGLYHMLENGKESLVRVYKANDYFGFRTFFGDNRYHCSARVLQPAELIRITPLNFIEF
                     WSNNSALIISLLKQLSTELQDAEHRLSMVSYQKTQERVMNSIHYLHQRYPNYHWTNRE
                     IAEFAGCETETAIRLRRELKKIS"
     gene            8914..10611
                     /locus_tag="PXD71_001169"
     CDS             8914..10611
                     /locus_tag="PXD71_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL6291301.1"
                     /translation="MLLTIITFLVVTGLVGYISWLKTRNDDLSTSKGYFLAGRGLSAT
                     VIGCSMVLTSLSTEQLIGVNANSYAGNFSIIAWTVPAVIPLCFLALYLLPKYIRNGYT
                     TIPEFFENRFDRQTRLIMSGLFLVFYLFIVIPTALYTGAIAFNKIFNLETAFGLSYGA
                     AITYTVIAIGIVGAIYAIFGGLKAVAVSDTWNAVLLVIGALLVPVFALIYLGNGSMAE
                     GVKIITTTHVEKWNAVGSATDAVPWPTIFTGIMVVHFFYWTTNQAIVQRALGARDLKA
                     GQKGILIAALFLLLLPLILNLPGLLSFHILGEGLKPIDVSYPELVNKTFPTVLQGFFI
                     AALFGAILSTFNSFLNSAATIYCKDLLPSISKKQFTDAELISYAKKVSAVMAVITMIF
                     GPLLMFGTDGIFLLTKRFAGFVNIPIVALFVIGLFNKTVSGKAARIALLAHVILYFCI
                     VWVFDVKINFVYVQAGLFVFDVALMLILGIFLKRATPYEDNKINHANVDLTSWEYAPL
                     AVASIVLGLFTLYAFLSPLGLASPDGNPAMVLAVYAVLQAVVCVVFFAKCKGKKAVN"
     gene            10758..12206
                     /locus_tag="PXD71_001170"
     CDS             10758..12206
                     /locus_tag="PXD71_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL6291302.1"
                     /translation="MNNIIYILLDQVRKDMLGTYGHQVVKTPNLDRLAKDGIRFNNAF
                     TPASVCGPARTSLFTGLMPSSHGIIKNGEKGGTGEIPQQNPNIAGVEGYNSFVIGKWH
                     VGTKSVPEDYGIAGHNFDGYGYPGSQVYKNLVFNQPPTHSNRYREWLAEKGYDAPEVS
                     RAYFGDNPHLRVQELCGLLSGSREQTIEWFIIDEAKKYIQASLDEKKPFFAWINFWGP
                     HTPCVVPEPYYSMYDPSQVVLDESFFKPLEGKPNHFRTISQMWGMWEASEDRWKEVIS
                     KFWGYITLIDDAIGELFRFLEENNLYENTFLVATADHGDAMGAHKMIEKGEFMFETTY
                     NIPLIIKDPHSTRVNEVDDNLVYLHDLTSTVYDVANQPIPTAFQGESILPITRQSQNN
                     DRKGLLCQLAGHFVYFEQRMWRRKDYKLVFNATDRCELYDVRKDPEEMHNLFYDEAYQ
                     AVKKEMLEEMRQEMKRLNDPLENWVYRIIDEI"
     gene            12296..13414
                     /locus_tag="PXD71_001171"
     CDS             12296..13414
                     /locus_tag="PXD71_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDL6291303.1"
                     /translation="MTTTYFHLMAKPSSFHCNIQCEYCFYLSKEQTIPPEKSQFMNDE
                     TLQNYIRHYIEANQSQRVDFVWQGGEPTLLGLDFYKQAVKYQQKFANGKQITNAIQTN
                     GIALNRQWASFFKENHFLVGLSIDGLSDIHDRYRISTNGKPTFERVKSAVDLLKEVGV
                     EFNTLTVVNDRNWQRGRETYQALKALGSRYMQFIPVVESHKGELQPFSVPPQGYGQFL
                     VDVFHEWLPDRGQIFVIEFDNLLGQWLGYPSNNCVFRPTCGSSLVVEACGDVYSCDHF
                     VYPDYHIGNLNQQQLAEIVSSAAQRQFGQNKQAKLSQACLNCKFRILCHGGCPKHRIV
                     PQANSENHNYLCPSYQQFFAETSSVMRQMSEQIRFAGK"
     gene            complement(13505..14086)
                     /locus_tag="PXD71_001172"
     CDS             complement(13505..14086)
                     /locus_tag="PXD71_001172"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL6291304.1"
                     /translation="MAKLYFYYSSMNAGKSTTLLQSSYNYQERGMNTLVYTAAIDDRF
                     GVGKVNSRIGISQEANLFTADTNLFAEIKALHSNKALHCILIDEAQFLTKTQVYQLSD
                     VVDKLKIPVLCYGLRTDFQAELFEGSKYLLAWADELEELKTICDCGKKAHFVVRLNEK
                     GEAIKDGEQIQIGGNDRYLSVCRYHYKQKLGKL"
     gene            complement(14142..>14348)
                     /gene="zapB"
                     /locus_tag="PXD71_001173"
     CDS             complement(14142..>14348)
                     /gene="zapB"
                     /locus_tag="PXD71_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HDL6291305.1"
                     /translation="VLDQLEEKIKQAVETIQLLQLEVEELKGKNDAAKQENDTLRSEY
                     EQLKVEQQNFQDRLRSLLGQIDNV"
BASE COUNT         4298 a         2855 c         3200 g         3995 t
ORIGIN      
        1 gtttaaatat ttcaggaaaa gtatatggag tctttgattc actaccaatg ggttgaaaca
       61 aatgagcaat tagaggccgt atgtgaacag gccagaaaaa caaaagtagt tgccttagat
      121 acagagttta tccgtacacg tacctattat ccgatattgg gtttaattca actctttgat
      181 ggccaacagg ttagccttat tgatccaaat acgatttctg acttctcgcc ttttgtgtca
      241 ttattagccg ataaaaatat cataaaagta ttacatgcct gtagtgagga tttagaagtt
      301 tttgagtatc aatttaagca attgccagag ccgatgttgg atactcaggt gatggcagcc
      361 tttgccggca ttggtatttc gatggggttt gcaaaattgg tttcccacta cttaaatatt
      421 gaactagaca aaggcgcttc ccgaaccgat tggcttgcca gaccgctaag cgctgaacaa
      481 ttacaatatg cggcagctga tgtttggtat ttattgccag tttatgagaa attggcacaa
      541 gcattaacaa aaagcacttg gcaaagtgct gtagcagagg agtgtgtcgc tttgttgaag
      601 aaagcgaaac agcctatcaa taaggaaaaa gcctataaaa atattgccaa tgcttggcga
      661 ttgaatcggg aagagttagc catattacaa gtattagcga aatggcggat tgaagaagca
      721 caaaagcgca atcttgcctt aaattttgtc gtgaaggagg caagtttagt tcaaatcgca
      781 aaaagctgtc ctaaacacac ttcttcgctg ttagaattta tgcacccaaa tgaagtcaga
      841 attcacggaa aaaaaatact atggttggtt gagcaaggaa aagcaataga atcgaatgat
      901 tatcctgaac tcattcagcg cttggtggat gaaaagggtt ataaatatta tatgcaagcg
      961 atgttgcaaa aattattaga aattcaaccg cttgatatcg taccggagtt ggttgcaagt
     1021 aaacgtcaat taaatcagct atttaaatgg tatattgatg gaaaaccatc agaaaaaatt
     1081 cctgaattat taatcggttg gcgaaaacct tttggtgaac agctgctatc ggttttatct
     1141 taaatttcat tcgggttaca tagactatgt acagtataat tgcctaaaaa ggagtttata
     1201 tggaaaataa aaaaccacca aaagcattag atgcgattga tttaaaaatc ttaaacgagc
     1261 tacaacgtaa cgggaaaatt tctaatattg aattatcaaa gcgagttggg ctttctccaa
     1321 cgccctgctt agaacgggta aaacggctag aaaaaaataa cgtaattatg ggctataaag
     1381 ctttacttaa ccctgaatta cttgatgccc ctttactggt gattgttgaa attactttgg
     1441 tgcgtggtaa gcctgatgta tttgaagaat ttaataaagc gattcaacaa cttgatgaaa
     1501 ttcaagaatg ccacctggta tcgggggatt ttgattatct gttaaaaacc cgtgtggcgg
     1561 atatggtatc gtaccgtaag ttattaggca caacattgct tcgtttgccc ggcgtaaatg
     1621 acactcgaac ctatgtggtg atggaagaag taaaacaaac caactatttg ttgttaaagt
     1681 aaggagaatt gggtgattga acaacttaaa ccacacctaa aaggcaaaga gaacttgatc
     1741 aaacttatgc ttttgttgac cggctttttg ggcgtttatt tgctaattgc ttgggcaagt
     1801 tatagtccat tagataatgc ttggtctgtt gcaagcagca ttacacaaac aaccttgaat
     1861 aaaacaggtc actttggtgc ttggagtatt gatatgctct atgcaatgtt tggtaaggtt
     1921 gcagttttag tacctattgc attaattatt ttctcaattt atagcttggg aatgggttta
     1981 agtgtcgata tgaaatggaa aactgtcttc attcgcctag ccagtttctt attattgatg
     2041 gtcggtcttg ccgggttatt tagcgtgctt ttttcaaact cagcttatta tctgtcgggt
     2101 ggctttgtcg gtggagtttg ggagacaata ttatctgata ctatcggaca atttggtgcc
     2161 ttattgattg caatgctttt tactatagct ggcttatatt tttgctctgt tcaatcatta
     2221 ttgccaatat taacgcaatt ttatgagtgg ataatggcaa aatcagaaga gagcaaaaca
     2281 gctgaatctc ttcagcattt gcaaaatact gagcaaaaac aaccgcttgt aactgaaaat
     2341 gaagcggtgg aagttgtaga tgaccataaa ccgcaattta ccgatatctc cgcctttaaa
     2401 cgcccaaaca ttagtggttt aagacactcg gaaaatgtag agatagataa ccgagaaatg
     2461 tatgaaattg aaagagatct tgagctaccg actattaata ttttaataca gcctgatatg
     2521 gttaaccaag ttgcggaaca agctacatcg ggctatgagt taccaaacga agaatccaat
     2581 gtattgcaaa tgccgaccat tcgcttaaat accgctatcg aaaacgaggt ggatacaatc
     2641 gaaaacagtt catcattgga agacctgaaa atagcagaat ctatgttacc tcaaattcag
     2701 cttgataatg atattgaaga tactgaagaa gctattgacg atgtgcgtga aattacaccg
     2761 gaatttatgg taacgccgcc caaggcgatg aaagatgtgt ctgagcagcc agcctatcca
     2821 aaaggctatg gtgatacgtt aattcacccg ttgttacaac gaaaagtgat aaccgaaaaa
     2881 ccgacaacac cattgccaac gctagatttg ttggataaag caccgattca aagccagcaa
     2941 attaccgaac aggaaatacg agaaacctct gtgcggctag aagcagaatt agcaaacttt
     3001 ggcgtaaaag ctaccgttga agatgtattg gtcggtccgg tagtgacccg ttatgaaatc
     3061 caaccggctg ccggggtaaa agcctccaaa attaccaatt tagcgagcga tatagctcgt
     3121 ggcttaatgt ttaaagcgat taggattact gaagttatcc caaacaaacc ttatatgggt
     3181 attgaaactc cgaataaaca ccgtgaaaca gtgtggttgc gtgatgtgct agacagcgat
     3241 gaattccgca ataccactgc aacattgcca atggcactag gtaaagacat cagtggtgag
     3301 cctgtggtgg tcgatatggc gaaaatgcca cacttactgg tggcagggca gaccggtggg
     3361 ggtaaatcgg ttggggtgaa tacgatgatt ttaagtctat tgttcaaact tacaccagaa
     3421 caagtccgct ttattatgat tgaccctaaa gtggtggagc tttccattta taacgatatt
     3481 cctcacttat taacgccggt agtaaccgat atgaaaaaag ccgccaacgc actacgctgg
     3541 gcggtggagg aaatggaacg ccgttatttg ctggtaagcc atttacaggt gcgtaatatt
     3601 gaaggctata atgcgaaaat cgaccaagcg gcagcgatga atttgccgat tccagatcca
     3661 acgtggcgac cgggcgattc aatggatagc cttccgcctc cgctacaaaa attaagctat
     3721 attgtgctga ttgtcgatga gtttgcggat ttaatgatgt ctgccggtaa agaagtggaa
     3781 gagtacatta tgcgaattgc tcaaaaagcc cgagcggtgg gaattcactt aattcttgct
     3841 acccaacgtc cgtcaaccga tgtgattacc ggcgtgatta aagccaatat tccaagccgg
     3901 attgcgttta cagtagcgag ccaaattgac tcaagaacta ttttagattc aggtggagca
     3961 gaggcattat tagggcgagg cgatatgctc tattcaggag ctggcagccc ggatattatt
     4021 cggatacacg gtgcgtttat gaaagacgaa gaagtgcaac gcgtggcgga taattggcgg
     4081 gcaagaggaa aaccaaacta cttggaaagc attgtggaat cccgctcgga agaggctgat
     4141 ggcaaaaacg atggcggaac gggcgattta gatccgctat ttgacgaggt tgttgaatat
     4201 atcacggaaa ccggttcggt ctctatcagt aatattcaac gccgcttctc gcttggtttt
     4261 aaccgtgcgg ctagaattgt ggatcaaatg gaagctcaag gcattgtttc tgagccactt
     4321 aaaggtggca aacgagaagt gctggcgagg taaaacacat acaagcggtt agatttctgt
     4381 gaaattttgc aaattttgac aaaaatctaa ccgattgtat ttatctaatg ctaaataagc
     4441 atagtataag gctagtcttt gggctaaaat taacgcaatt tttacatctt ttaaggttga
     4501 atatatgcga ttagatgata ttaaagaaag taaaaatgtt gaagacagaa gattcaacaa
     4561 tgtgggtaga actaaggttg gtagagggaa aggtggaata ttaaccttta ttatcgtgct
     4621 tgttggagct tattatggtg ttgacttaac aggtttaatg ggcggtggcg tagaatatga
     4681 ggagcagaca tcagcattaa atgaaagtga agaaaaacag ctaagaacct tatccgctaa
     4741 agtattaacg accacagaaa atatttggac ttcctacttc cagcaaaatg gtctaactta
     4801 taaagagccg tctttggtgc tttaccgtgg ggcaattcaa actgcctgtg gcaccggtca
     4861 atctgcaatg ggtcctttct attgccctgt cgatcaaaag gtttatttag atttatcatt
     4921 ctatgatgat atgcgtaaaa aattaagagc atcgggcgat tttgccttct cttacgtgat
     4981 tgcacacgag gttggccacc acgtccaaaa tttattgggc ataacaagca aaacccaacg
     5041 ggctcaaatg aaagcctcat ctaaagccga agcaaaccaa atttcagtca atgtagaatt
     5101 gcaagctgac tgttttgccg gcgtttgggg acaccaactc gcaaaagcca accgattaga
     5161 ggaaggcgat gtggaagaag cctttaatgc tgcccaagca gttggagatg accgattaca
     5221 gcaacaaagt cgtggctatg tcgtgccgga tagcttcacc cacggctcat ctgcacagcg
     5281 tttggcttgg tttagaaaag gtttaaccac aggaaatcct gcagtttgta atacatttaa
     5341 ttaaaaaata attaaattat ttgaaatctt tttattatcg tactactatg atagtacgat
     5401 tttattttca ttttatttag agagtgaaca caatgtctta tatcaaaaat gacgatgtca
     5461 gaattaccag tattgaagaa ttactaccac ctattgcatt actggaagcc tatccggcaa
     5521 gtgatgttgc agcggaaacc gttgctaaaa cccgtcaatc aatccacaaa attttacacg
     5581 gtgcggatga tcgcttatta gtggtgattg gaccttgctc cattcacgac ccgaaagcgg
     5641 cattagaata tgcacaaaaa attaaagcaa tgcgtgctga tccgaaaatt aaccaaaact
     5701 tagaagtggt gatgcgtgtc tattttgaaa aaccccgtac aaccgttggt tggaaaggct
     5761 taattaacga cccttatttg aacgaaacct ttgccttaaa cgatggttta cgcattgccc
     5821 gtaaagtatt atctgatatt aatgatttaa gcgtgccgac tgccggcgag tttttggata
     5881 tgattacacc acaatatgtg gctgacttta tgagctgggg agcaattggg gcgagaacga
     5941 ctgaatctca agtccaccgt gaattagcct ctggtttatc ctgtgcggta ggttttaaaa
     6001 acggcacgaa tggtagtgtg aaaattgcat tagatgcaat ttcttccgca aaatcgccac
     6061 accatttctt atcggtcact aaattcgggc attctgcgat tgtttcaaca gcaggaaatc
     6121 ctgactgtca cattattttg cgtggtggcg atagcgcaac caactacgat gctgactccg
     6181 ttgcagcggc ttgtgctgcg attgagaaat ccggcggacg tccacacgtg atgatcgatt
     6241 tcagccacgc taacagccag aagcagttca aacgccaaat ggacgtttgt gctgatgtgt
     6301 gcaaccaaat tgcaggtgga tcagacttta tttcaggtgt gatgattgaa agccacttgg
     6361 tagaaggtcg ccaagactta attgaaggca aaggtttaga ggcgttagtt tacggacaaa
     6421 gtattacaga cggctgcatt ggctgggaag atagtgaaaa agcgttattc gcacttgctg
     6481 aagcggttga aaagcgcaga aattttgcaa aataatcttt tcttgaaccg agggcgaata
     6541 taattcgccc tttatttgtt taaccttaaa gcaaatttaa tttattcaaa ctaaacatat
     6601 gatgaaaccc agcattcttg ctttagatac tgcaacagag gcttgctctg tagcgttaca
     6661 gtataacgga aatatcacgt tcttagatga aatcagccct cgttcccata ctcaacgtat
     6721 tttgccgatg gtagatgaat tacttaatca agccaatatc gaactcaaag aagtaaatta
     6781 tttagtattc ggacgtgggc cgggcagttt taccggtgtg agggtcggtg taagtgtggc
     6841 acaagggttg gcaatgggag cgaatttgcc ggtggtagct gtttctaact taaaagcaat
     6901 ggcagaagaa gcataccaaa aattaggtgc aacaaaggta attgccttaa ttgatgcccg
     6961 aatgaatgaa gtttactttg ctcaatttga acgaaagggt gaagaatgga atgaagtggt
     7021 ggctgaacaa gtctgttcgc cagaagtcgc aataagtcaa ttccaaatga gtgaagagac
     7081 tgtggtggtt ggcacaggtt gggcggcata ttcgcaattt tctgagcaaa atctaccgct
     7141 tgttatcagt gatattacct tgccttccag ccgatatatg ctttcgattg ctgaaactga
     7201 gattcaaaaa ggcaatatcc aatctgcttt tgaaatagaa ccggtgtatt tgcgtaatga
     7261 agttacttgg gaaaaactac ctcataagca ataatctatt gaacttatgg ttaagtgaat
     7321 aaatattgca aaaagcccat ataacaggca atagtgaata ggattacacc aagtaattta
     7381 gtagttaatt cgggactcaa gatattgcgt aatttcattg aaaaatagac agtaaagaga
     7441 gaacttatca ctacggtgcc gacaattgca aaattcacat agccgatttg ccaagttgct
     7501 aaggttgaat tcaacacaaa agggcgggat aagtagccat ataaattgcc gattccgcca
     7561 atcaccatca tatagttagt ataaactgca acctgtttcg gtgaaaccga gcgtatttgc
     7621 gtgacaatcg gagccataat cgaaccgccg ccaatgccgg taatacctgc aattaaaccg
     7681 cctaaactgc acattgtggc attagatact gcttcttttg gtttaggtgg tactattgct
     7741 ttaactcttg gtttgtgaaa tagggtttta aatgaaagtg tcagcagggt tagaataaag
     7801 atagcgataa tgctgttatc agagaaaaga aaactgagtt caaacccaat ttgtacgcct
     7861 aacatcatcg caagtgacca aatcaacaca ttttttcgat tgatttttat ctgttgatgg
     7921 tggaataagc ataaattgat agtggctgaa ccaatcacaa tggtaagaga gcaggcggaa
     7981 atcacttgga gaggcagttc aggaaacagt gaatgtaaaa tcggcatcat taaaacaccc
     8041 ccgccaatcc caaatagtgc agaagtgata ttgactataa tgccacaaat gatcaaaata
     8101 attatcacgg tcattgtcat aatccatctc ctaaagtgtg ataagtatgc aaaattttct
     8161 aaataaagga ttatgatcgc aatcatatat ggaaaaaata aaataataag gataatcaat
     8221 gaacaatatg ccaacccccg aacaattgat gtcttgccaa ttaagtgtaa tgtctgatct
     8281 agaaaaagga gtcgtcattt attctcaggg ttatcacgcc agagaatttt attttttaaa
     8341 aagcggatta atagggctgt accatatgct tgaaaatggt aaagagagct tggtgagggt
     8401 atataaagcc aacgattatt ttgggtttag aacctttttt ggcgataatc gctaccattg
     8461 ctcggctcgg gtgttacagc cggcagaatt gatacgtatt acccccttaa atttcattga
     8521 gttttggtcg aataattccg ccttaattat ctctctgtta aaacagctct cgacagaatt
     8581 acaagacgca gagcatcgtt tatcaatggt ttcctatcaa aaaacgcaag agcgggtaat
     8641 gaacagtatt cattatttac accaacgtta tcccaattat cactggacta accgtgaaat
     8701 cgccgaattt gcaggttgtg aaaccgaaac agccattcga ttaaggcgag aattaaagaa
     8761 aatcagttaa attgtcataa attattcata gttgtgatca ccatcacata tctattatga
     8821 caaacatcat attctatcgt taaaaataag atttatgcta ttgccgtctc taatgggata
     8881 tatcatttat atagtcatat tgaggtgaag aatatgttat taacaataat tacctttctg
     8941 gttgtaactg gattagtcgg gtatatctct tggctaaaaa ccagaaacga cgatctttcg
     9001 acatctaaag gttatttctt ggcagggcgt ggcttgagtg ctacggttat cggctgttcg
     9061 atggtcttaa cctccctttc taccgagcaa cttatcggcg tgaatgccaa ctcttatgcc
     9121 ggtaatttct caattattgc gtggactgtg ccggcggtaa ttccgctttg cttcttggca
     9181 ctttatctgt tgccgaaata tatccgcaac ggttatacca ccattccgga gttctttgaa
     9241 aaccgtttcg accgccaaac ccgtttgatt atgtcgggct tgtttttggt gttctatctt
     9301 ttcatcgtca ttccgactgc actttacacc ggtgcgattg ccttcaataa aatcttcaac
     9361 cttgaaactg ccttcggctt aagctatggg gcagcgatta cctatacggt gattgcgatt
     9421 ggtattgttg gggcgattta tgccattttc ggtggcttaa aagcggtggc ggtgtcagac
     9481 acttggaatg cggtcttgtt agtcattggg gcattattag tgccggtgtt tgcacttatc
     9541 tatttaggta atggcagtat ggcagaaggg gtgaaaatca tcaccactac tcacgttgaa
     9601 aaatggaatg cggtgggtag tgccaccgat gcagtgcctt ggccgaccat tttcaccggt
     9661 attatggtgg tgcatttctt ctactggacg accaaccaag ccattgtgca acgtgccttg
     9721 ggggcgagag acttaaaagc cggtcaaaaa gggatcttaa ttgcggcact gttcttatta
     9781 ttgctaccgc ttatcttaaa cttaccgggc ttactcagtt tccatatttt aggtgagggc
     9841 ttgaaaccga ttgatgtgtc ttaccctgaa ttagtgaata aaactttccc aaccgtgcta
     9901 caaggcttct ttattgccgc actgttcggg gcgattttga gtaccttcaa ctcgttctta
     9961 aactcagcgg caaccattta ctgtaaagac ttactgccgt ctatcagcaa aaaacagttc
    10021 acagatgcgg aactgataag ttatgcgaaa aaagtctctg cggtaatggc ggtgattacg
    10081 atgatctttg ggccgttact gatgttcggc acagacggta ttttcttgct gaccaaacgt
    10141 ttcgccggtt ttgtgaacat tccgattgtg gcactgtttg tcatcggctt attcaacaaa
    10201 accgtctcag gcaaagccgc acgcattgcc ttactcgcac acgttattct ctacttctgc
    10261 atcgtttggg tgtttgatgt aaaaatcaac ttcgtgtatg tgcaagctgg cttgttcgtg
    10321 ttcgatgtgg cgttaatgct gattttagga attttcctaa aacgtgcgac tccatacgaa
    10381 gacaacaaaa tcaaccacgc caatgtggac ttaaccagct gggaatatgc cccactggca
    10441 gtggcttcta tcgtgttggg cttattcacg ctatacgctt tcctctctcc gctcggttta
    10501 gcctcaccag acggcaatcc ggcaatggtg ctggcggtgt atgctgtgct acaagcggtg
    10561 gtttgtgtcg tgttttttgc aaaatgtaaa ggcaaaaagg cggtgaatta aatgaatgtg
    10621 agccttctcc tcattagtat aaaaatacca agaacggaaa aggctcgaga tcaaaaatcc
    10681 cctctttgct aaagagggga acagatctcc gaaacttgat aatttatcgg ttattaacaa
    10741 gccttaaaga ggtcattatg aataacatca tctatatttt gttagatcaa gtgcgtaaag
    10801 atatgctcgg cacttacggg catcaggtgg taaaaacccc gaatttggat cgcttggcga
    10861 aagacggcat tcggtttaac aacgccttta cgccggcttc ggtctgcggg ccggctcgca
    10921 cctcgctgtt taccggctta atgccgtcca gccacgggat tatcaaaaac ggcgaaaaag
    10981 gcggcacagg cgaaattcca caacaaaacc ctaatattgc cggcgtggag ggctataact
    11041 cctttgtgat cggcaaatgg cacgttggta caaaatccgt tcccgaagat tatggcatcg
    11101 ccggtcataa ctttgacggc tatggctacc cgggcagcca agtgtataaa aacctcgtgt
    11161 ttaatcagcc gccaacacac tccaaccgtt atcgtgaatg gctggcggaa aagggctatg
    11221 acgcacccga agtcagtcga gcctattttg gcgacaatcc acacctgcgt gtgcaggaac
    11281 tctgtggttt gctttccggc agtcgcgagc agacaataga gtggtttatc attgacgagg
    11341 cgaaaaaata cattcaagcc tcccttgatg agaaaaaacc gttctttgcg tggatcaact
    11401 tctgggggcc gcacactccg tgtgtagtgc cggagccgta ttattcaatg tatgacccaa
    11461 gccaagtggt attggacgag agtttcttca aaccgcttga gggcaaaccg aaccacttcc
    11521 gcaccatttc acaaatgtgg ggaatgtggg aggcgagcga agatcgttgg aaagaggtga
    11581 tttctaaatt ctggggctac atcacgctga ttgacgatgc cattggcgag ctgttccgtt
    11641 tcttggaaga gaacaatctc tatgaaaata ccttcctcgt tgctaccgcc gaccacggcg
    11701 atgcgatggg ggcgcataag atgatcgaaa aaggcgagtt tatgtttgaa accacctaca
    11761 acattccgct gattattaaa gatccgcact ctacaagggt gaacgaagtc gatgacaacc
    11821 tcgtttattt gcacgatctc acttccaccg tgtatgatgt ggcaaatcaa ccgatcccga
    11881 ccgctttcca aggcgagagt attctgccga taaccagaca aagccaaaac aacgaccgca
    11941 aaggcttgtt gtgccaactg gcagggcatt ttgtctattt tgaacagcga atgtggcggc
    12001 gtaaggatta caaattggtc ttcaacgcca ccgaccgctg cgaactctac gatgtcagaa
    12061 aagaccccga agagatgcac aacctctttt atgacgaagc ctaccaagcc gtgaaaaaag
    12121 agatgttgga agaaatgcga caggaaatga aacgcctcaa cgacccactg gaaaattggg
    12181 tgtatcggat tattgatgag atataattgt ttgtagaggc gggttttata cccgcccgaa
    12241 aattaatgta aacaatgggc gaacataaag tccgccccta caatttagag atacaatgac
    12301 caccacctac ttccacctaa tggcaaaacc cagcagtttt cactgtaata ttcagtgcga
    12361 gtactgtttt tatctgagca aggaacagac cattccgcca gaaaaatcgc agtttatgaa
    12421 cgatgaaacg ctgcaaaact acattcgtca ttacattgaa gccaaccaaa gccaacgggt
    12481 ggattttgtg tggcaggggg gcgagccgac cctgctgggc ttagatttct acaaacaagc
    12541 ggtcaaatat caacaaaaat ttgcaaacgg caagcagatc accaacgcaa tccaaaccaa
    12601 cggcattgcc ctaaaccgcc agtgggcgag ctttttcaaa gagaatcatt ttctggtggg
    12661 cttatccatt gacggactga gcgacattca cgaccgctac cgcatttcca ccaacggtaa
    12721 accgaccttt gaacgggtga aaagtgcggt cgatttattg aaagaggttg gcgtggaatt
    12781 taacaccctc acggtggtga acgaccgcaa ctggcaacgg ggcagagaaa cctatcaagc
    12841 cctcaaagcc ctcggtagcc gttatatgca gttcattccg gtggtggaaa gccacaaggg
    12901 ggaattacaa cccttttccg ttccgcccca aggctacggg caatttttag tggatgtttt
    12961 tcacgaatgg ctgcctgacc gtgggcagat ttttgtgatc gagtttgata acctgctcgg
    13021 gcaatggctt ggctatccct ccaacaactg tgtattccgc ccgacctgtg gcagctcgct
    13081 ggtggtggaa gcctgtggcg atgtgtacag ttgcgaccat ttcgtctatc ccgattacca
    13141 catcggcaac ctcaaccaac agcagctggc agagatcgtc tcatcagccg cccaacgcca
    13201 attcgggcag aataaacagg caaaactctc gcaagcctgc ctaaactgca aattccgcat
    13261 actttgccac ggcggctgcc ccaaacaccg catcgtcccc caagcaaaca gcgaaaacca
    13321 caactacctt tgcccctctt accaacagtt ttttgccgag acttcgagtg tgatgagaca
    13381 aatgagtgag caaattcggt ttgcaggaaa gtaaagagag gtaaataaca caaacaagcg
    13441 gttaaatttc gctgaaaatt tgcaaaaatt cccgaaaatt taaccgcttg ctgcttcttg
    13501 tagcttatag cttacctaac ttttgtttgt aatgataacg gcaaacagag agatatctat
    13561 cattgccgcc gatttgtatt tgttcaccgt ctttaatggc ttcgcctttt tcatttaatc
    13621 gaacgacaaa atgggctttt ttgccacaat cacagatggt tttgagttct tccagctcat
    13681 ctgcccaagc caacaaatat ttgctacctt caaataattc agcttggaaa tcggtgcgta
    13741 gcccgtagca aagcaccgga attttgagtt tatcgaccac atcacttaat tgataaacct
    13801 gtgttttagt taaaaactga gcctcatcaa tcaagataca gtgtaacgct ttgtttgagt
    13861 gtaatgcctt aatttccgca aatagattgg tatcagcggt aaataaattg gcttcttgtg
    13921 aaataccgat acgagagttg actttcccca caccgaaacg gtcatcaata gcggcggtat
    13981 aaactaaagt gttcattcca cgttcttggt aattataaga agattgaagc agagtagtag
    14041 atttgcctgc attcattgag gagtagtaaa aatagagttt tgccatagct gtaacaaata
    14101 aaaaacggct aaaaatttag ccgtttgtgc tgaaaataga attaaacgtt gtcgatttga
    14161 cctaaaagtg aacgtaaacg atcttggaag ttttgttgtt ccacttttaa ttgttcatat
    14221 tcgctgcgca aggtgtcgtt ttcttgtttc gctgcatcgt ttttaccttt taattcttca
    14281 acttctaatt gaagtaattg aatagtttca acagcttgtt taattttttc ttctaattgg
    14341 tctaaaac
//
LOCUS       DANLXS010000055        14106 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.055, whole genome shotgun sequence.
ACCESSION   DANLXS010000055 DANLXS010000000
VERSION     DANLXS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14106
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.055"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            113..1984
                     /gene="rpoD"
                     /locus_tag="PXD71_001174"
     CDS             113..1984
                     /gene="rpoD"
                     /locus_tag="PXD71_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL6291306.1"
                     /translation="MENQADQQLEQQSQLELLISQGREQGYLTLSEVHDHLPEELVDA
                     EQIEDIIQMINDMGIQVLEAAPDADDLLLAENIADEDVVEEATKVLSTVESELGRTTD
                     PVRMYMREMGTVELLTREGEIDIAKRIEEGINEVQCAIAEYPEALSGLLACYAQVETG
                     EMRLSDLVTAFVDPNILEEAPLEDIEAALGEDEESSDVPDVDDENEDENGDSSSDSSD
                     DSSIDPELAREKFEALRSQHEKVLLVIQKHGRTSKKSKEQIEILSHIFREFRLVPKQF
                     DLLVASMQKLMKQVRTEERQIQRYAVEYAGMKKADFLKAFQGHELSEAWIEKAINAKK
                     NAAPKLANYVDNIRVNIANLQNLEQTSGLTIAQIREIGGRISNGELKARRAKKEMVEA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKLNRISRQCLQEMGREATPEELAERMGMPEDKIRKVL
                     KIAKEPISMETPIGDDDDSHLGDFIQDESLELPLDSATAESLRMATNEVLEGLTPREA
                     KVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDD"
     gene            2078..3064
                     /locus_tag="PXD71_001175"
     CDS             2078..3064
                     /locus_tag="PXD71_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL6291307.1"
                     /translation="MKKSLLALLIGSVFLISACEDGKMTQTVLEAEKQIVQLGTELKN
                     AQADLAKKEAEVAELSGVKAENEKLKSELENAQQNTALNVEIVKIFDKKEVIKHQVDP
                     KQEFAIEESTVSAFVSLPKTNFDWLNQLLINQAYDYNEEQGRKLKNPTEEEFKKHREA
                     VYQGLVESVKTEPAIGYDDHISSAFIGQRNNIATFTMMHYTYMGGAHGMHYTQYINVD
                     LAKKAIIGLNDLISPKNQVQLKDILWENYSSVRVDENGNYNGFADKKEFRISDQFYFT
                     PHGIAFVYPPYELGAYAEGDIEVEADWYIINKLLNSEYQRGEKDGFFEELDN"
     gene            complement(3140..4048)
                     /locus_tag="PXD71_001176"
     CDS             complement(3140..4048)
                     /locus_tag="PXD71_001176"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL6291308.1"
                     /translation="MNKNSLFSIWFTTARPKTLPLALASIIVGSALAHWAGKFDVVTT
                     LLAFITTILLQILSNFANDYGDHVKGSDTKERIGPLRAIQHGVIGGEQLKKAVIILSL
                     LSFVSGVALSAYAFETVQDLFFFIGLGIVSIIAAITYTVGKKAYGYLGLGDLFVLIFF
                     GFVAVIGVFYLQAHSLPIMILLPAFGCGLLSVAVLNINNLRDINQDRQAGKNTLIVRI
                     GSQNGRKYHVALLVLAVISYLIFAISEFEHWYSFIFLLSVPLLAKHARFVYQHKDPKE
                     LYPILGQMAGLALLTNLLFSLGIVLG"
     gene            4228..4965
                     /gene="tsaA"
                     /locus_tag="PXD71_001177"
     CDS             4228..4965
                     /gene="tsaA"
                     /locus_tag="PXD71_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDL6291309.1"
                     /translation="MNSTSNQLTPIQLNPIGFVESPYDEKFSVPRQPNLVAEGKGILR
                     LIPPYNTPDAVRGLEKFSHIWLIFQFHHIPEREWHATVRPPRLGGNERVGVFASRATH
                     RPNPLGLSKVKLEAVVAGGSEVYLKLGSVDLVNGTPIFDIKPYIAFADSEPQAVSGFA
                     QQKPQARLAVRFSEEAKQAVEFCRNFAKFGIEQPLSFLADVIAQDPRPAYQQGKPSER
                     VYGMNLAGYNIRWQIENDTQAIVLSIE"
     gene            5023..5742
                     /locus_tag="PXD71_001178"
     CDS             5023..5742
                     /locus_tag="PXD71_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253715.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDL6291310.1"
                     /translation="MKLKNFLTILPLAVATAVTSAETHTVEHKDKPASFNFSTEVSRT
                     VEKDVMQASVYSQKSGNSLPELKKFVSENLNRVLAEAKKHPSIEVQAEGVTNHVNYNN
                     KGNIDGWDTSGSIQLKSKDFDAMAAVLENLGKDVAIRYIDFSVSPEKMASLEDEMTLE
                     IISKFQHKADVIQKALGAKTYKLVNINIQTPNDGGRPYYEHRAMYAVSSMAQSKSMDE
                     IPLEAGKTTISATASGSVEFE"
     gene            5930..6616
                     /gene="aqpZ"
                     /locus_tag="PXD71_001179"
     CDS             5930..6616
                     /gene="aqpZ"
                     /locus_tag="PXD71_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDL6291311.1"
                     /translation="MKKYFAEFFGTFWLVFGGCGSAVLAAGIPELGIGYAGVSLAFGL
                     TVLTMAYAVGHISGGHFNPAVSIGLLVGGRFNAKDLVPYIIAQVIGAIAAGAVLYTIA
                     SGVPTFDATAGFAGNGYGEHSPHGYSLTAALLIEVVLTAFFLIIIMGATDKRAPAGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTGVALFQGSWAIEQLWLFWVAPIVGAIIGA
                     VVYRFIAEEK"
     gene            6688..6828
                     /locus_tag="PXD71_001180"
     CDS             6688..6828
                     /locus_tag="PXD71_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291312.1"
                     /translation="MKKLIKVCGLISATIFLSGCLVTSVVGGVVGTAVDVVDTVTPDI
                     ID"
     gene            6931..7524
                     /gene="slyD"
                     /locus_tag="PXD71_001181"
     CDS             6931..7524
                     /gene="slyD"
                     /locus_tag="PXD71_001181"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL6291313.1"
                     /translation="MNITKDLVPSIAYQVRTQEGVLVDEAPVNQPLEYLHGHNNLVEG
                     LEKALEGKAVGDVFEVRVKPEEGYGEYNDNMVQRVPKDVFMGVDELEVGMRFIADTDL
                     GPLPVVITEVDGDEVVVDGNHMLAGQELLFSVEVVGIREATAEEIAHGHVHGGHEHGC
                     GCGGHDHHHDHDGCCGGHDHDHHHHHGHGGCGCGGHH"
     gene            7785..9317
                     /gene="ilvA"
                     /locus_tag="PXD71_001182"
     CDS             7785..9317
                     /gene="ilvA"
                     /locus_tag="PXD71_001182"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDL6291314.1"
                     /translation="MMNETQHSQSAIDYLRSILSSNIYDLAQVTPLQKMEKLSERLGN
                     SIFIKREDRQPVHSFKLRGAFAMISNLSKAQKEAGVIAASAGNHAQGVALSAKHLGLR
                     ALIVMPQNTPAIKVDAVRGYGGEVLLYGANFDEAKAKAIELSTELGMTFIHPFDSPAV
                     IAGQGTIGLELVQQKPDLDYVFIPVGGGGLIAGIAVLIKQVMPNIKVIGVESKDSACL
                     YHALKQGQPTDLERVGLFADGIAVKRIGDETFRLCQQYVDDVVLVDNDEICASMKLIF
                     ENVRAISEPSGATSLAGLKKYVKEHQLQDKNLACILSGANVNFHTLRFVSERCEIGEK
                     REALLAVTIAEQKGSFLKFCEILGNRAVTEFNYRHSDDKQACIFVGVRISGSAEKVEI
                     IKDLQQNGYDVADLSDDDIAKTHIRYMVGGRASSIQYEQLYSFEFPEQKGALLKFLQM
                     LTNWDISLFHYRAHGADYGDILAAFALNRGDEVELNRHLEQLGYRFQNVSESPAYQYF
                     LK"
     gene            9343..10677
                     /gene="glmM"
                     /locus_tag="PXD71_001183"
     CDS             9343..10677
                     /gene="glmM"
                     /locus_tag="PXD71_001183"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL6291315.1"
                     /translation="MARKYFGTDGVRGEVGKFPITPEFALKLGWAAGKVLATQGTKKV
                     LIGKDTRISGYMLESALEAGLTAAGLSAVFVGPMPTPAIAYLTRTFRAEAGIVISASH
                     NPYYDNGIKFFSSVGEKLPDEIEEAIEAMLDKPMDCVDSANLGRASRINDAAGRYIEF
                     CKGTFPSELSLEGYKIVVDCANGATYHIAPNVMRELGAEVLEIGTKPDGLNINEKCGA
                     TDIKALQKVVVEAGADVGLAYDGDGDRLIMVDHLGNKVDGDQILFILAREALRSGKLQ
                     GGVVGTLMSNMSLELALKELAIPFSRANVGDRYVLEQLKEKGWKLGGENSGHIIVLDK
                     NTTGDGIIASLQVLAAMASHKLSLNDLAKAVPLFPQVLLNVRFEKGAANPLESNEVKA
                     VAADVEKRLAGKGRILLRKSGTEPLIRVMVECEDAVLAQSCAEEIVEAVKRN"
     gene            10822..11481
                     /gene="rpiA"
                     /locus_tag="PXD71_001184"
     CDS             10822..11481
                     /gene="rpiA"
                     /locus_tag="PXD71_001184"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL6291316.1"
                     /translation="MDQQEMKKIAAQAALKFVKPDTIVGVGSGSTVNCFIDALASMKD
                     EIKGAVAASKASEQRLRDIGIEVFSANEVTELDVYIDGADEITPQGAMIKGGGAALTR
                     EKIVSSLAKKFICIVDKSKQVDVLGSTFPLPVEVIPMARSYVARQLVALGGSPEYREG
                     VVTDNGNVILDVHNFKIMEPLKMEHTINNIAGVVTNGIFAQRFANVTIVGTPEGAVIL
                     E"
     gene            11518..12747
                     /gene="serA"
                     /locus_tag="PXD71_001185"
     CDS             11518..12747
                     /gene="serA"
                     /locus_tag="PXD71_001185"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL6291317.1"
                     /translation="MAKISLDKSKIKFLLLEGVHQNAIDVLLAAGYTNIEVHKKALDG
                     QELIDAIKDAHFVGIRSRTFLTEEVLAQAPKLIAIGCFCIGTNQVDLNAAKRRGIPVF
                     NAPFSNTRSVAELVLAEMILLMRKVPTANAEVHRGVWNKSAAGANEVRGKKLGIVGYG
                     HIGSQLSILAEAIGMQVYFYDVENKLPLGNAQQVPTLEHLLSTCDMISLHVPEIDSTK
                     NLMSAERIAQLKEGSVLINAARGTVVDLDALAARIEDGSLRGAAIDVFPEEPASINDP
                     FVSPLCKFDNVILTPHIGGSTSEAQANIGTEVANKFVKYSDNGSTVTAVNFPEVSLPL
                     HTNTKRLLHIHENRPGMLNQINQIFVESNVNIAAQYLQTDAAIGYVVIDVESDNVDEA
                     LQRLKAIEGTIRARVLY"
     gene            complement(12825..13343)
                     /locus_tag="PXD71_001186"
     CDS             complement(12825..13343)
                     /locus_tag="PXD71_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDL6291318.1"
                     /translation="MKKLTIVALTCLFSTHLLAAPVGNTFTGIGVGVDVTTTKYKVEG
                     VKGKDSTGPALVVDYGIEQGNNFVGVVQGKAKVGSTKVFSDVKQKNKYTIAYQQGYRV
                     GSDLLPYVKVDASSSKIGNETYRGFGFGAGAKYAVSSDVELGAEYTRDNLKRSGTKLK
                     GNAFTANATYRF"
     gene            complement(13363..14085)
                     /gene="arcA"
                     /locus_tag="PXD71_001187"
     CDS             complement(13363..14085)
                     /gene="arcA"
                     /locus_tag="PXD71_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDL6291319.1"
                     /translation="MKIPQILIVEDELVTRNMLKSIFEGEGYQVFQASNGEEMHSVLS
                     TNEINLVLMDINLPGKNGLILAREIRENMKLPLMFLTGRDNEIDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLQRTMIDKENDVVKEQSEIESYRFNGWILDINSHSLISPEGEV
                     NKLPRSEFRALLHFCQHPGKIQTREDLLKKMTGRELKPHDRTVDVTIRRIRKHFEDHP
                     NTPEIIVTIHGEGYRFCGNIEP"
BASE COUNT         4253 a         2651 c         3233 g         3969 t
ORIGIN      
        1 aaagtaatgt gtaaattaga aaaatagaat ttttttgtga atttatgaag tcgaaccaaa
       61 tttagtggta taatccacta ctatttttgc tcgcataata aacggataga ctatggaaaa
      121 ccaagcagat caacaattag aacagcaatc tcaattagaa cttctgatca gtcaagggcg
      181 tgaacaggga tatttaacgc tttctgaagt gcacgaccac ttaccggaag agttggtgga
      241 tgcggagcag atcgaagata ttattcagat gatcaacgat atgggtatcc aagttttaga
      301 ggctgcccct gatgcggatg atttactact cgctgaaaat atcgccgatg aagatgtggt
      361 agaagaagcc accaaagtgc tttcaaccgt agaatcagag ttaggtagaa ctactgaccc
      421 tgtgcgtatg tatatgcgtg agatggggac agttgagctt ttaacccgtg aaggtgagat
      481 tgatatcgct aaacgcattg aagaagggat taacgaagtt caatgtgcga ttgcggaata
      541 tccggaagcc ttaagtggcc tgttagcctg ctatgctcaa gtcgaaaccg gtgaaatgcg
      601 cctttctgat ttagtgacag catttgtcga tcctaatatt ttagaagaag caccgttaga
      661 ggatattgag gctgctttag gtgaagatga agaatcttct gatgtgcctg atgtagatga
      721 cgaaaacgaa gatgaaaacg gtgatagcag ttcagacagc agtgatgaca gctcaattga
      781 cccggaactt gcccgtgaaa aatttgaagc cctacgcagc caacatgaaa aagtgttatt
      841 ggtcattcaa aaacacgggc gaaccagtaa aaaatcgaaa gagcaaattg aaattttatc
      901 tcatattttc cgtgaattcc gtttggtgcc gaagcaattt gacttattgg ttgcttctat
      961 gcaaaaactg atgaagcagg ttcgcactga agaacgtcaa attcaacgct atgcagtgga
     1021 atatgccggc atgaaaaaag cggatttctt gaaagcattc caagggcacg aattaagcga
     1081 agcgtggatt gaaaaagcga ttaatgccaa gaaaaatgca gcaccgaaat tagctaatta
     1141 tgtggataat atccgtgtca atattgctaa cttacagaat ttagagcaaa catcaggctt
     1201 aaccattgct caaattcgtg aaattggcgg gcgtatttcc aatggtgagt taaaagcccg
     1261 ccgtgcgaaa aaagagatgg tggaagctaa cttacgtttg gtgatttcga ttgcgaaaaa
     1321 atacactaac cgtggcttgc agttcttaga tttaatccaa gaaggaaaca tcggtttaat
     1381 gaaagcggtg gataaatttg aataccgccg tggttataaa ttctcgactt atgcaacttg
     1441 gtggattcgt caagcgatta cccgttcgat tgctgaccaa gcccgtacaa ttcgtattcc
     1501 ggtgcatatg attgaaacta tcaacaaact caaccgtatt tcacgccaat gtttacaaga
     1561 aatggggcgt gaggcaacgc cggaagaact ggcagaacgt atgggaatgc cggaagataa
     1621 aatccgcaaa gtattgaaaa ttgccaaaga gccgatttca atggaaacac caatcggtga
     1681 cgatgacgat tcacatttag gtgatttcat tcaagacgaa agccttgaat taccattgga
     1741 ttccgccact gcggagagcc tgcgtatggc aacgaacgaa gtgttagagg gcttaacccc
     1801 gcgtgaagcg aaagtgttgc gtatgcgttt tggtattgat atgaacacag accacacact
     1861 cgaagaagtt ggtaaacaat ttgacgtaac tcgtgaacgt atccgccaaa ttgaggcgaa
     1921 agcactgcgc aaacttcgcc acccgagccg ttcggaaacc ttacgcagtt tcttagacga
     1981 ctaatcattt cccctatgcc tgatggtata ggggattttg ttttaatctg tataacaagc
     2041 ggtgatattt accgtatttt ttacaaaaga gggagagatg aaaaaatcac tattagcact
     2101 tttaattggc tcagtatttc tgatttcagc ttgtgaagac ggcaaaatga cccaaacggt
     2161 attagaagcc gaaaaacaaa ttgttcagtt aggcacagaa ttaaaaaatg ctcaggctga
     2221 tttagcaaaa aaagaggctg aagttgcaga actttcaggg gtgaaagcag aaaatgaaaa
     2281 attaaaatca gagttagaga acgctcagca aaacacagcg ttaaatgttg aaattgtgaa
     2341 aatctttgat aagaaagagg tgattaagca ccaagttgat ccgaaacaag aatttgcaat
     2401 agaagaaagt acagtaagtg cgtttgtgtc tctaccaaaa accaattttg attggcttaa
     2461 ccagcttttg attaatcagg cctatgatta caatgaagaa caaggcagaa agctgaaaaa
     2521 tccaaccgaa gaagagttta aaaagcatcg tgaagcggtt tatcaagggt tggtagaaag
     2581 tgtgaaaacc gagccagcta ttggctatga tgatcatatt tccagtgctt ttatcgggca
     2641 acgcaataat attgctacat tcactatgat gcattacacc tatatgggtg gggctcacgg
     2701 tatgcactat acgcaatata tcaatgtaga tttagctaaa aaagcaatca ttggcttaaa
     2761 tgatttaatc agcccaaaaa atcaagttca actgaaagac attctttggg aaaattacag
     2821 tagcgtgcgt gtggatgaaa atggcaacta taatggattt gctgataaaa aagagttccg
     2881 tatttctgac caattctact ttacccctca cggcattgct tttgtttatc cgccatacga
     2941 attaggtgct tatgcagaag gcgatataga ggttgaagcg gattggtaca ttattaataa
     3001 gttgctcaat tcggagtatc aacgtggcga aaaagatgga ttctttgaag aactagataa
     3061 ctaattgtaa atttagataa aaaacaaccg cttgtaatcc tttattacaa gcggtttagt
     3121 tttgttaaaa ttttgcaact taccctaaca caatgcccaa actaaataat agattagtga
     3181 gcaacgccag ccctgccatt tgccctagaa ttgggtagag ttcttttggg tctttatgct
     3241 gatagacaaa acgagcgtgt tttgccagta acggtacaga cagcaaaaaa atgaagctat
     3301 accaatgttc aaattcagaa attgcaaaaa tcagataaga aatgaccgct agcactaaca
     3361 atgcaacgtg gtattttcgc ccgttttggc taccgattcg tacaatgaga gtgtttttcc
     3421 cagcttgtcg gtcttggtta atatcacgca agttattgat atttaacact gctacagaca
     3481 gcaatccgca accgaaagca gggagcagaa tcattatagg taggctatga gcctgcaagt
     3541 agaacacgcc gattaccgct acaaagccaa agaaaatcag cacaaataga tcgcccagcc
     3601 ccaaatagcc gtaggctttt ttgccgacgg tgtaagtaat ggcggcaata attgagacga
     3661 tgccaagccc gataaagaaa aacagatcct gcactgtttc aaaagcatag gcacttaagg
     3721 caacaccgga aacaaacgag agtaggctta agataatcac ggctttctta agttgttcac
     3781 cgccaataac accgtgttga atcgcccgta gcggtccgat tcgttctttg gtgtcagagc
     3841 ctttgacgtg gtcgccgtaa tcattggcga agttagacag gatctgcaac aaaatggtgg
     3901 tgataaatgc gagtaaggtg gtaacaacat caaatttccc tgcccaatga gcaagtgctg
     3961 aacccacaat aattgaagct aacgccagcg gtaaagtttt cgggcgagcg gtggtaaacc
     4021 aaatcgaaaa gagagaattt ttgttcatag agaataatct tagcgtaatt tcatttacaa
     4081 tgatgttaag gatttaagcg atagtgcaag cgtccactct tgtgttaaat ggtaaaacga
     4141 attggtacaa gcgtggacgc ttgcaccatc ctcaatttca tatcattatg tccaatattt
     4201 tatattattt taacagaaaa aagcgtaatg aactctacct caaatcaact aaccccgatt
     4261 caattaaacc ctatcggttt tgtggaaagc ccctatgatg aaaaattttc cgtgcctcgc
     4321 cagccgaatt tggtagcgga ggggaaaggc attctacgtt taattccgcc ttacaatacc
     4381 cccgatgcag tcagaggctt ggagaaattt agtcatattt ggttgatttt ccaattccat
     4441 cacattcccg aaagagagtg gcacgccacc gtcagaccgc ctcgcttggg cgggaacgaa
     4501 cgggtcgggg tctttgccag ccgtgccacc catcgtccga atccgcttgg cttatcgaaa
     4561 gtgaagttag aagcggtagt ggcggggggg agtgaagttt atttgaaatt aggcagcgtg
     4621 gatttagtta acggcacacc aatttttgac atcaagcctt acattgcatt tgcagatagc
     4681 gagccgcaag cggtttccgg ttttgctcag caaaaaccac aagcaagatt agcggtgcgt
     4741 tttagcgagg aggcaaaaca agctgtcgaa ttttgccgaa attttgcaaa atttggcatc
     4801 gaacagccgc ttagttttct tgccgatgtc atcgcccaag accctcgccc ggcgtatcaa
     4861 caagggaaac ctagtgagcg agtttatggt atgaaccttg ccggctacaa tattcgctgg
     4921 caaattgaaa atgatacaca ggcgattgtg ctttcgattg aatgaacttt cctacgcaag
     4981 tgagatctaa gaaatgattt ttaacccttt taaggactga ttatgaaatt gaaaaatttt
     5041 cttaccattc taccgcttgc agtcgcaaca gcggtaactt cagcggaaac tcataccgtg
     5101 gaacataaag ataaaccggc ttcttttaat ttctcaaccg aagtctctcg cacggtggaa
     5161 aaggacgtga tgcaggcaag tgtgtatagc caaaaatcag gtaactcctt gccggagctg
     5221 aaaaaatttg tatctgaaaa cctgaatcgt gtattggctg aggcaaaaaa acatccatcg
     5281 attgaagtac aagctgaagg tgtgactaat cacgttaatt ataacaacaa agggaatatt
     5341 gatggttggg atacctctgg tagcattcag ttgaaaagca aagattttga tgcaatggca
     5401 gcggtgttgg aaaatttagg taaagatgtg gcgattcgtt acattgattt tagtgtatcg
     5461 ccagagaaaa tggcgtccct tgaagatgaa atgacgctgg aaattatcag caaatttcag
     5521 cataaagccg atgtgattca aaaagcctta ggggcgaaaa catataagct agttaatatc
     5581 aatattcaaa ctccaaatga tggtggtaga ccatattatg aacacagagc aatgtatgca
     5641 gtttcatcaa tggcacaaag caaatctatg gatgaaatcc cgttagaagc aggtaaaacc
     5701 acaatttcag ctaccgcttc aggaagtgtg gaatttgaat agaaatgtat agatttgtat
     5761 agagttgtat ttttacttta attggaggaa aaaatcggtc tatactatca attaagaaat
     5821 atgcgtcttt acttttttta gtatactcat attttattgg ctatgtttta tagcattcgg
     5881 aaagttaata cacttatgtg tgattcaccc tttagggtaa ggataaaaaa tgaaaaaata
     5941 ttttgctgag tttttcggga cattttggtt agtgtttggt ggttgcggca gtgcagtttt
     6001 agcagcaggc attcctgaat taggaatcgg ttatgcaggc gtttcgttag ccttcggttt
     6061 aaccgtatta acaatggcgt atgcagtcgg tcatatttca ggtggtcact tcaacccggc
     6121 ggtttcaatc ggtttattag tcggcggtcg ttttaacgct aaagatttag tgccttatat
     6181 cattgcccaa gtaatcggtg cgattgctgc cggtgcagtg ttatacacca ttgcttcagg
     6241 tgttccgact tttgatgcta cagcaggctt tgccggcaac ggttatggtg agcattcgcc
     6301 acacggctat agcttaacag cagcattatt aattgaagtg gtattaacgg cattcttctt
     6361 aatcatcatt atgggtgcaa ccgataaacg tgctccagca ggtttcgctc cgattgctat
     6421 tggtttggct ttaaccttaa tccacttaat cagcattccg gtaaccaata cctcagtaaa
     6481 cccggcacgt tcaaccggtg tagcgttatt ccaaggcagc tgggcgattg agcaattatg
     6541 gttgttctgg gttgctccga ttgtcggtgc aattatcggt gcggtagttt atcgctttat
     6601 cgctgaagaa aaataattta agcgaataaa tatgatgcag ctctagtctt gattagggct
     6661 gttttttgtt tttgaaagga ttttaaaatg aaaaaattaa tcaaagtatg tgggctgatt
     6721 tcagcaacta ttttcctaag tggttgtttg gtaacttcgg tggttggtgg agtggtggga
     6781 accgcagttg atgtggtaga caccgtaacc cctgatatta tcgactaatt tactgacaag
     6841 cggttaaaat ttccagatag attgcaaata taaaataaag cgttacaatg gcttgctatt
     6901 ttataaaacc gatgaattaa aggattcaat atgaacatta caaaagattt agtgccaagc
     6961 attgcatatc aagtgcgtac tcaagaaggc gtattagtag atgaagcacc ggtaaaccaa
     7021 ccgttagaat atttacacgg tcacaacaac ttggtagaag gcttagaaaa agcccttgaa
     7081 ggcaaagctg taggcgatgt gtttgaagtg cgtgtgaaac cggaagaagg ttacggcgag
     7141 tacaacgata atatggttca acgtgtaccg aaagatgtat ttatgggcgt cgatgaatta
     7201 gaagtgggta tgcgttttat tgcggacacg gatttaggtc cgctaccggt ggtaattacc
     7261 gaagtagatg gcgatgaagt tgtggtggac ggtaaccata tgttagccgg tcaggaatta
     7321 cttttctctg tggaagtggt tggcattcgt gaagcgacag cggaagaaat cgcacacgga
     7381 cacgttcacg gcggacacga acacggttgc ggttgtggtg ggcacgatca tcatcacgat
     7441 cacgatggtt gctgcggcgg tcacgaccac gatcatcacc atcatcacgg acacggtggc
     7501 tgtggttgcg gtggtcatca ctaactttgt gatttcccct ctccccgctt gcgggagagg
     7561 gtggagcaaa tcgaagattt gcggagggag agggggatat ccaaggtttc ccctctctct
     7621 gcttaaaatt tctgaacgaa attttaagct gtctctctcc cgcaaacggg cgagaggaaa
     7681 tattccctgt taaagccatt gctaaataac caaacaagcg gtcaattttc cttggaaatt
     7741 taccaatgtc tttatctcaa tctaacaata aataaccttt caaaatgatg aacgaaacac
     7801 aacattccca atccgctatc gactatttac gctcgatttt aagctcaaat atttatgact
     7861 tagcgcaagt gaccccttta cagaaaatgg aaaaactgtc tgagcggtta ggcaactcta
     7921 tttttatcaa acgtgaagac cgacagccgg ttcacagctt taaattgcga ggtgcttttg
     7981 cgatgatctc aaatttatcc aaagcacaaa aagaggctgg agtcattgcg gcttcagcag
     8041 gcaaccacgc acaaggggtg gcactttctg ccaaacattt aggtttaaga gccttgattg
     8101 taatgccgca aaacacaccg gcaattaagg ttgatgcggt acgtggctat ggtggtgaag
     8161 tgttgcttta tggggcgaat tttgatgaag ctaaggcgaa agcgattgag ctttccaccg
     8221 aattgggtat gaccttcatt cacccgtttg acagcccggc agtgattgca gggcaaggca
     8281 caatcggctt agaactggtg caacaaaagc cggatttaga ttatgtattt attccagtgg
     8341 gcggtggcgg cttaattgcc ggcattgcgg ttttaattaa gcaggtgatg ccgaatatta
     8401 aagtgattgg ggtcgaatct aaagattctg cttgcttata tcacgcctta aagcaagggc
     8461 aaccgaccga tttagaacgg gttgggcttt ttgctgacgg tattgcggta aaacgcattg
     8521 gcgatgaaac tttccgcctg tgtcagcaat atgttgatga tgtggtgttg gtggataatg
     8581 atgaaatctg tgcctcaatg aagttgattt ttgaaaatgt gcgtgcgatt tccgagccgt
     8641 ccggggcaac ttcccttgcc ggtttgaaaa aatatgtgaa agagcatcaa ttacaagata
     8701 aaaacttagc ttgcattctt tccggggcga atgtgaattt ccacacctta cgttttgtgt
     8761 cagagcgttg tgaaatcggg gagaaacgtg aagctttatt ggcagtcacc attgctgaac
     8821 agaaaggtag tttcttaaaa ttctgcgaga ttttaggcaa ccgtgcggta acggaattta
     8881 actatcgcca ttccgatgat aaacaagcct gtatttttgt gggggtgcga atttcaggct
     8941 cggcagaaaa agtagagatt attaaagatc tgcaacaaaa cggctatgat gtggcagatt
     9001 tatcggacga tgatattgcc aaaacccata ttcgttatat ggtaggcggt agagcaagct
     9061 ccattcaata tgagcaactc tatagctttg aatttccaga gcagaaaggg gcattgctaa
     9121 aattcttaca aatgctgact aactgggata tctcgttatt ccattaccga gctcacggag
     9181 ccgattatgg cgatattctt gccgcatttg cattaaacag aggcgatgaa gtagaattaa
     9241 accgacattt agaacagcta ggttaccgct tccaaaatgt gagcgaaagt ccggcatatc
     9301 aatatttttt aaagtaaatt tttatcaatc ttaggaatta gaatggctcg taaatatttc
     9361 ggcacagacg gtgtgcgtgg tgaagtaggt aaatttccaa ttacacctga atttgcatta
     9421 aaactcggtt gggcagcagg taaagtgttg gcaacccaag gcacgaaaaa agtattaatc
     9481 ggtaaagata cccgtatttc cggttatatg ttggaatcgg ctctggaagc cggtttaacc
     9541 gcagccggtt tatcagcagt attcgttggt ccgatgccga ctccggcaat tgcttattta
     9601 acccgtactt tccgtgcgga agccggcatt gtgatttctg cctctcacaa tccttattac
     9661 gataacggca ttaaattctt ctcgtcggtt ggtgaaaaac tacctgatga aattgaagaa
     9721 gcgattgaag caatgcttga taagcctatg gattgcgtag attcagcaaa tttaggtcgt
     9781 gccagtcgta ttaatgatgc cgcaggacgt tatatcgagt tttgtaaagg cactttcccg
     9841 tctgaactaa gccttgaagg ctataaaatt gtggtggatt gtgccaacgg tgcaacctac
     9901 catatcgcgc cgaatgtaat gcgtgagcta ggggcagaag tgcttgaaat cggtactaag
     9961 ccggacggtt taaacatcaa cgaaaaatgt ggtgcaacgg atattaaagc acttcaaaaa
    10021 gtggtggtag aagcaggtgc ggatgttggt ctagcttacg atggtgatgg cgaccgtttg
    10081 attatggtcg atcacttagg caataaagtt gatggcgacc aaattctatt tattttagcc
    10141 cgagaagctc tacgttcagg caaattacaa ggcggtgtag tcggtacatt aatgagtaat
    10201 atgagcttgg aattagcttt aaaagagcta gccattccat tctctcgtgc taacgtgggt
    10261 gaccgctatg tactagagca gcttaaagaa aaaggctgga aactgggtgg cgaaaactcc
    10321 gggcatatta tcgtgttaga taaaaacacc accggtgatg gtattattgc ctcactgcaa
    10381 gtattagcag caatggcaag ccacaaatta agcctgaatg atttggcaaa agcagtgcct
    10441 ctgttcccgc aagttctgct aaatgttcgc tttgagaaag gggcggcaaa tccgcttgaa
    10501 agtaacgaag tgaaagcggt agcggcagat gtggaaaaac gcttggcagg caaagggcgt
    10561 attttattgc gtaaatcagg cactgagcca ttaattcgag tgatggtgga gtgcgaagat
    10621 gctgtacttg ctcaaagctg tgcggaagag attgttgaag cagtaaaacg taactaatga
    10681 caagcggtcg tttttggtaa aaaatttgcc aaatatgacc gcttgttaca tatatgtgta
    10741 gaatagatgt agtagggcga aaattttttt cgccttgccg aagtgggcaa atgatgattt
    10801 gccctacaag tggaggtaaa aatggatcaa caagaaatga aaaaaattgc ggcacaagct
    10861 gcattaaaat ttgtaaaacc ggacactatt gttggggtgg gaagtggttc aaccgtaaac
    10921 tgttttattg atgcccttgc ttcaatgaaa gatgagatta aaggagcggt tgctgcctct
    10981 aaagcctcgg aacagcgttt gcgtgatatc ggcattgaag tattcagtgc caatgaagtg
    11041 acggaattag atgtgtatat tgacggagct gatgaaatca cgccacaagg ggcgatgatt
    11101 aaaggtggcg gggcagcact tacccgtgag aaaatcgtga gttcattagc gaaaaaattt
    11161 atctgcattg tggataaatc caaacaagtt gatgtgctgg gttcaacttt ccctctgccg
    11221 gtggaggtga tcccaatggc tcgttcttat gtggctcgtc agctcgtagc cttaggtggt
    11281 tcgccggaat accgtgaggg cgtggtcact gataacggca atgtgatttt agatgtacat
    11341 aactttaaaa ttatggagcc gttgaaaatg gaacatacca ttaataatat tgcaggcgtg
    11401 gtgactaacg gtatttttgc acaacgtttt gccaatgtaa ccattgtcgg cacacctgaa
    11461 ggtgcagtta ttttagaata atttattatt gcaaatacaa atacttagga gcaaaaaatg
    11521 gctaaaattt ctttggataa atccaagatc aaattccttc tgcttgaggg cgtacatcaa
    11581 aacgcaattg atgtactctt agcggctggt tataccaata ttgaagtgca taaaaaagca
    11641 ttagacggtc aagaattaat tgatgcaatt aaagatgcac actttgttgg tattcgctcg
    11701 cgtacttttt taaccgaaga agtactggct caagctccga aattaattgc gattggttgt
    11761 ttctgtatcg gtacaaacca agttgatttg aatgcggcga aaagacgagg tattccggta
    11821 ttcaatgcac cgttctcaaa tacccgctcg gtggcagaat tagtgctggc agagatgatt
    11881 ttattaatgc gtaaagtccc gactgcaaat gcggaagtgc atcgtggggt gtggaataaa
    11941 tcggcagcag gtgcaaacga agtacgtggc aagaaattag gtattgtggg atatggtcat
    12001 atcggttcac agcttagtat tcttgccgaa gccattggta tgcaggttta tttctatgat
    12061 gttgaaaaca aactaccact cggtaatgca cagcaagtgc cgacattaga gcatttgctt
    12121 tcaacctgcg atatgatttc actacacgtt ccggaaatcg actcgactaa aaacctaatg
    12181 agtgcagaac gcattgctca attaaaagaa ggctcggtgt taattaatgc cgctcgtggt
    12241 acagtggtcg atttagacgc attagccgct cggattgaag acggctcatt gcgtggtgcg
    12301 gcgattgacg tatttccgga agaaccggca tcaattaatg acccgttcgt gtcaccattg
    12361 tgcaagtttg ataacgtgat tttaacgcca catattggtg gttcaacctc tgaagctcag
    12421 gcaaatatcg gcaccgaagt tgccaataaa tttgtgaaat attcagataa cggttcaacc
    12481 gtgacggcag tgaatttccc tgaagtatcc ttaccgcttc atactaatac caaacgttta
    12541 ttacatattc acgagaatcg cccaggtatg ttaaatcaaa ttaaccagat ttttgtggaa
    12601 tcaaatgtga atattgccgc tcaatattta caaaccgatg cagctatcgg ttatgttgta
    12661 attgatgtag agagcgacaa tgttgatgaa gcattacaac gtttaaaagc gatcgaaggc
    12721 acgattcgag cccgagtatt atattaaaag gcaaagagcc tgaatcactt caggctcttt
    12781 tgtgttacaa gcggttattt tttcagaaaa ctttaccgct aagattagaa acgataagtt
    12841 gcattcgcgg tgaaagcgtt accttttaat tttgtgccgc tacgttttaa attatcgcga
    12901 gtgtattctg cacctaattc aacatcgctt gaaactgcat attttgcacc agcaccgaaa
    12961 ccaaagccac gataggtttc attaccgatt ttgcttgagc tggcatctac ttttacataa
    13021 ggtaataaat cggatccaac acgataacct tgttgataag ctatagtata tttatttttt
    13081 tgttttacat cgctaaatac ttttgtacta cctacttttg cttttccttg tacaacgcct
    13141 acaaagttat tgccttgctc aataccataa tcgacaacta atgccggacc tgtggaatcc
    13201 ttgcctttta ctccttcaac tttgtatttt gttgtagtta catctacacc gacaccgatg
    13261 ccagtaaatg tatttccgac aggagcggct aatagatggg tagaaaataa gcaagttaat
    13321 gcgacaatag ttaatttttt cataaaatta ctcctatctt gattaaggtt cgatgttacc
    13381 acagaaacgg tagccttcac cgtgaatggt gacaataatt tctggggtat tggggtggtc
    13441 ttcaaaatgt ttacgaatac ggcgaatagt aacatcaacg gttctatcat gaggttttag
    13501 ttcccgcccg gtcatttttt tcaataaatc ttcacgtgtt tgaatttttc caggatgttg
    13561 gcaaaaatgt aataaagccc taaattcact acgtggtaat ttatttacct caccttctgg
    13621 tgagattaaa ctatgactgt taatgtctaa gatccaaccg ttaaagcgat agctttcaat
    13681 ttcagattgc tcttttacaa cgtcgttttc tttatctatc atcgttcgtt gcaataaatt
    13741 tctcgctcgg atagtcaatt ctcgaggatt aaacggttta gtgatatagt catcggcacc
    13801 tatttctaaa ccgaggatct tatcaatttc attgtcgcga ccggttaaaa acattaatgg
    13861 tagcttcata ttctctcgaa tttctcgggc gagaattaat ccattttttc ccggtaagtt
    13921 aatgtccatt agcacaagat tgatttcatt tgtactcaga acagagtgca tttcctcgcc
    13981 gttagatgct tgaaatacct gatatccttc tccttcaaaa atgcttttca gcatattcct
    14041 tgttactaac tcatcctcaa caattaagat ttgtgggatc ttcatctttt aaacctctga
    14101 tttaat
//
LOCUS       DANLXS010000056        14055 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.056, whole genome shotgun sequence.
ACCESSION   DANLXS010000056 DANLXS010000000
VERSION     DANLXS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14055
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.056"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            95..1072
                     /locus_tag="PXD71_001188"
     CDS             95..1072
                     /locus_tag="PXD71_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL6291320.1"
                     /translation="MSRNLFQKRLNIKPYEYPELLEFKDAIRHSYWLHTEFNFTGDIQ
                     DYRTTINDHERHVLTRAMLAISQVEVNVKRFWGELYCYFPKPEMDDVGGTFAESEVRH
                     KDAYSFLLEKLGLNEMFSQIHEIEPLMNRIRYMESFMKDKDAGKGEFVLSLVLFSLFV
                     EHISLFGQFIIMMSFNKHKNLFKGISNAVEATSKEEEIHGKFGIALYEILRDEHSELF
                     TPEFFEDLQTLANQAFEAERGILDWIFEEGDLSFITKATVENYIKNRYNNSLSILGLE
                     PPHDIDPELLKETEWFDIEILSTKETDFFNKRSTDYSKKMKQITADDLF"
     gene            1059..2726
                     /locus_tag="PXD71_001189"
     CDS             1059..2726
                     /locus_tag="PXD71_001189"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL6291321.1"
                     /translation="MTYFNTDRPDFHWLNEDSRLFLQRGYLLEGTTALDRIRFIAEHA
                     ERKLGIEGYADKFYHYMARGYFSLSSPIWSNFGLDRGLPISCFGSYIGDSIHEIMVTT
                     AEVGMMSKIGGGTSAYFGDIRPRGSLIKNNGKSDGSFNFSKLFDTVIDVISQGTSRKG
                     QFAGYIDIEHGDIDEWLDIHTEGNPIQLMYYGVCVGHDWLESMKAGDPYKRQLWAKLL
                     QRKTETGIPYLFFKDNANAGRPDVYKDKNMTIHASNLCTEIMLPSSNEESFVCCLSSM
                     NLLYFDEWKDTDAPETLTYFLDVVMSEFIEKSQSIPFLDRANRFARRHRALGLGVLGW
                     HSYLQANNIAFDSFQAMQKNNEIFKTLQAKTLKASQELAQRFGEPEILEGYGRRNTTL
                     MSIAPTKSSSFILGSVSPSVEPFKSNYYVKDLAKIKTVYKNPFLEKLLQEKGLDTEEI
                     WTSILQNDGSVQHLEQLSEHEKEVFKTFAEISQLSVIQQAAQRQKYIDQGQSINVMIH
                     PATPARDLNQLYLTAEELGLKSIYYQNSMSAAQVFNRNLLSCSSCEG"
     gene            2730..3149
                     /locus_tag="PXD71_001190"
     CDS             2730..3149
                     /locus_tag="PXD71_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291322.1"
                     /translation="MGNLSHTSPNTIVEVVGEFPEITSSGYTFDKGPIQLRIGKHTAP
                     NKGFGVKHIEAEHMQEILNLGFTSVNEFVKAIIVENAKIFCEFSDLKGNHRPIILKAS
                     IGIVVLERKFLESGTIYSVVTAYRRTNPHGIQIGTMK"
     gene            complement(3384..4145)
                     /gene="nudC"
                     /locus_tag="PXD71_001191"
     CDS             complement(3384..4145)
                     /gene="nudC"
                     /locus_tag="PXD71_001191"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDL6291323.1"
                     /translation="MNKGYWIIVSDFDIALEAGEIPFGSAEELGLSGREKLTIGQYQG
                     SPAYLVKALESDNQREFAYLRSQINRPPELSHLLHRAVSLNHFFQTHQFCGKCGEKTI
                     LASDEIAIYCNACGNRAYPTISPSIIVAVRKGRQILLANHLRHKGTIYTTLAGFVEAG
                     EAVETAVHREVFEETGIKVKNVRYFGSQPWAFPNSLMLGFLADYESGEIQLQEEEICD
                     AKWFDCEESLPEIPQQGTIAFELIQATLKLCRESK"
     gene            complement(4138..4821)
                     /locus_tag="PXD71_001192"
     CDS             complement(4138..4821)
                     /locus_tag="PXD71_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL6291324.1"
                     /translation="MKQPTFKRMTNKRSNFAKNLQKNPQKRPLTFAETTLVLLNKPYD
                     VLTQFTDEQGRKTLKDFITMPNIYPVGRLDRDSEGLLLLTNNGEIQHRLAHPKFDKQK
                     TYWVQVEGVPQPEDLAKLAKGVELKDGLTKPAKVRAIATPDFAWNIAPQIRERKTIPT
                     SWIELIISEGKNRQVRRMTAHIGFPTLRLIRVGIGQFRLGNLGCGEFQVLSENEKRKL
                     FTQLGLKDE"
     gene            complement(4818..5666)
                     /locus_tag="PXD71_001193"
     CDS             complement(4818..5666)
                     /locus_tag="PXD71_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6291325.1"
                     /translation="MEKRTLALLKVHGTAVLFGASGIFGALIQSSAETIVLGRVMIAF
                     AIISLYFLWKKQPLVKLNLQQMAQLAFSAALLAAHWVTFFVAVKVGGVAVATLGFASF
                     PAFVALFESLFFREKLRFREVVLLIAITVGLILVTPEFTFGNQATQGLLWGVLSALVY
                     GILAVANRRTMSKISGVQASWWQYIFATLFLLPLSITEFPSVSTQDWFWIFCIGFLCT
                     TLAYTLFISSLDTINARTASMIISLEPVYAIVIAWLCFNEVPTLKMIIGGLIILLSVA
                     WANLKK"
     gene            complement(5666..6151)
                     /locus_tag="PXD71_001194"
     CDS             complement(5666..6151)
                     /locus_tag="PXD71_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HDL6291326.1"
                     /translation="MPILAILFGIFFYIYCEISLLVNIGSIIGVLPTILLLVVISFLG
                     LWIVKLRGVYTLFSIKQDLSQGKIPTEAVGNSVMFIVAGILLIIPGFLSDILAILCVL
                     PFSRKWIQAFVINSVKNKVFMHFTAQTSSFSVTSAQDSNTFDAEFERKPDEQDQHNRI
                     K"
     gene            complement(6153..7475)
                     /gene="pepQ"
                     /locus_tag="PXD71_001195"
     CDS             complement(6153..7475)
                     /gene="pepQ"
                     /locus_tag="PXD71_001195"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDL6291327.1"
                     /translation="MKNLFSTHLARLQQTVQSLLENAFLDGLWIYAGKAKTLFLDDQT
                     APFKINPYFNYFFPYPQAENCWLFLDGKNKPKIYFYAPEDYWVSTLKVPTEAFFAAEF
                     EWVLLRAENEIATFIQNPTACAFIGEDVELAKKLGFSHINPQKVLNSLNFARSIKTEF
                     EIESIYQAQFSALKGHQVAKEAFFAGKSEFEINLEYLKATQQSDLNVPYGNIVAINQN
                     GAVLHYTALDFDTHAKRHSFLIDAGTTVNGYASDLTRTYAFDSGSEFAELVAKMEQLK
                     LQIIAELSIGVNYLHYHTRMQQMISQLLAEHQFIHLSAEQIFEQGISRTFFPHGLGHL
                     LGIQVHDVGGFQQNARGSHKAPPEVYPSLRCTRELQENMVLTIEPGFYFIEMLLKPWK
                     NSALSSAFNWQKIEAFKAFGGIRTEDNIVMRANGAENLTANAAATLGG"
     gene            7486..7728
                     /gene="tusA"
                     /locus_tag="PXD71_001196"
     CDS             7486..7728
                     /gene="tusA"
                     /locus_tag="PXD71_001196"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDL6291328.1"
                     /translation="MTQLTVNKELDTLGLRCPEPVMLTRKTIRNMEEGEILHIIADDP
                     ATTRDIPSFCEFMDHQLIQKEVDSTPYHYWIKKGLE"
     gene            complement(7795..8226)
                     /gene="rraB"
                     /locus_tag="PXD71_001197"
     CDS             complement(7795..8226)
                     /gene="rraB"
                     /locus_tag="PXD71_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDL6291329.1"
                     /translation="MYNLDELRTETDETISSLLADGSDPEALYIIEHHVAHHDFDKLE
                     KLVVDAYKLGYEISEAEEVEDENGKVIFVCDIVSEIHLNADLITAQQKELLPLIEKAG
                     AEYEGWGTYFEDPNADEDEYGDDGEFFDDENDDFEDEPRRH"
     gene            complement(8241..8852)
                     /locus_tag="PXD71_001198"
     CDS             complement(8241..8852)
                     /locus_tag="PXD71_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL6291330.1"
                     /translation="MKLNYHKTHFLTSAPDIRHLPEDTGVEIAFAGRSNAGKSTALNA
                     LTNQKSLARTSKTPGRTQLINLFKVETNCKLVDLPGYGYAAVPEQMKIQWQKALGEYL
                     QKRECLGGVVILMDIRHPLKDLDQQMIEWAVSANLPVLLLLTKADKLSQSARSKQVKM
                     VREAILPFQGDIQVEAYSALNKIGIDKLSNKLDSWFAPKLITE"
     gene            complement(8854..9270)
                     /locus_tag="PXD71_001199"
     CDS             complement(8854..9270)
                     /locus_tag="PXD71_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL6291331.1"
                     /translation="MSWYKALFSFNGRLNRRGFWIGFGIHFLFLFMVAMWINLAELSL
                     LSVLPLIITGFSLSAIIIKRLHDRNRSAKALLMALVPIICYLAAPYSQGIMQILLGQV
                     MPAFIGMLLFLEWGVFAGNPEPNQYGEQGLSFKFRE"
     gene            complement(9274..10104)
                     /locus_tag="PXD71_001200"
     CDS             complement(9274..10104)
                     /locus_tag="PXD71_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL6291332.1"
                     /translation="MFEYFLSLSPIWQAFLAGLFTWGCTIFGSSFVYFFKSVNRKLLD
                     SMMGFAAGVMIAASFWSLLAPSLEYAEADYGSLAWLPAAIGFLAGGAFIRLIDYVVPH
                     LHLSKPIEQAEGNDQLKQGLSKSTLLFLAITIHNIPEGLAIGVAFGALATQVSGVDAS
                     IMGAIGLAIGIGLQNIPEGSSLVLPIRGEGHSRKKAFWYGSMSAVVEPIAAVIGAVFV
                     LSMTAILPYALAFAAGAMIFVVVEELIPESQSSGNTDIATLSLMLGFVVMMVLDVALG
                     "
     gene            complement(10441..10556)
                     /gene="rrf"
                     /locus_tag="PXD71_001201"
     rRNA            complement(10441..10556)
                     /gene="rrf"
                     /locus_tag="PXD71_001201"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(10718..13616)
                     /locus_tag="PXD71_001202"
     rRNA            complement(10718..13616)
                     /locus_tag="PXD71_001202"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(13859..13934)
                     /locus_tag="PXD71_001203"
     tRNA            complement(13859..13934)
                     /locus_tag="PXD71_001203"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13898..13900),aa:Glu,seq:ttc)
BASE COUNT         4076 a         3284 c         2677 g         4018 t
ORIGIN      
        1 aaaaagagtg tttgtcaaga cttgtcttga aacttttttt gcatacaatc atctaccagt
       61 tccaaacata aactttaaga tagagcctac gaatatgtct agaaacctct tccaaaaacg
      121 cttaaatatt aagccgtatg agtatcctga gcttcttgaa tttaaagatg caattcgcca
      181 ttcttactgg ttacacaccg aatttaactt caccggcgat attcaagact atcgcaccac
      241 cattaacgat cacgaacgtc acgtgttaac ccgagcaatg cttgcgattt cacaagtaga
      301 agtgaatgta aaacgtttct ggggcgagct atactgctat ttcccaaaac cggagatgga
      361 cgatgtaggc ggtacttttg ctgaaagtga agttcgccat aaagatgctt actcgttcct
      421 attggaaaaa ttaggcctaa atgaaatgtt cagtcaaatt catgaaattg aaccattaat
      481 gaaccgcatt cgttatatgg aaagttttat gaaagataaa gatgctggca aaggcgaatt
      541 tgttttatcg ttagtgctgt tctctttatt tgttgagcat atttcattgt ttggacagtt
      601 tattatcatg atgtcgttca acaagcataa aaacctgttt aaagggattt ccaatgcggt
      661 tgaagccacc tcaaaagagg aagaaattca cggtaaattc ggaattgcgt tatacgaaat
      721 tttacgtgac gaacacagtg aactattcac accagaattt tttgaagatt tgcaaacctt
      781 ggcaaatcaa gcgtttgaag cagaacgtgg catcttagac tggattttcg aagaaggtga
      841 tttaagcttt atcaccaaag cgacggtaga aaattacatt aaaaaccgtt acaacaattc
      901 tttaagcatt ttagggttag agccgccaca cgacatcgac cctgaattat taaaagagac
      961 tgagtggttt gatattgaaa ttctttcaac caaagaaacc gatttcttta acaaacgtag
     1021 caccgattat tctaaaaaaa tgaaacagat taccgcagat gacttatttt aatacagacc
     1081 gcccggactt ccactggctt aatgaagaca gccgtttatt cttacaacgt ggctatttat
     1141 tggaaggaac aaccgcatta gaccgcattc gttttattgc cgaacacgca gagcgaaaac
     1201 tcggcattga aggctatgcc gataaattct atcactatat ggcaagaggc tacttctcgc
     1261 tttcttcgcc aatttggtca aactttggct tagatcgtgg cttaccgatt tcttgtttcg
     1321 gtagctacat cggcgactct atccatgaaa ttatggtgac taccgctgaa gtgggcatga
     1381 tgagtaaaat tggcggtggt acttcggcct actttggcga tattcgtccg cgtggctcgt
     1441 taattaaaaa caacggtaaa tcagacggct catttaactt cagtaaatta tttgacactg
     1501 tgattgacgt gatttcacaa ggaacatcac gcaaaggtca gtttgccggt tatattgata
     1561 ttgagcacgg cgatattgac gaatggctgg atattcacac cgaaggcaac ccaattcaat
     1621 taatgtatta cggtgtgtgt gtcggtcacg attggttaga gtcaatgaaa gccggcgatc
     1681 catacaaacg tcagctttgg gcaaaattgt tacaacgtaa aaccgaaacc ggtatccctt
     1741 atttattctt taaagacaat gccaacgcag gtcgtcctga tgtatataaa gataaaaata
     1801 tgacgattca cgcctctaac ttatgtactg aaattatgtt gccttccagc aacgaagaaa
     1861 gtttcgtatg ctgtttatcg tctatgaacc tactctactt tgatgagtgg aaagataccg
     1921 atgcaccaga aacattaacc tatttcctcg atgttgtaat gagtgaattt attgagaaaa
     1981 gtcaatcaat tccattctta gaccgtgcaa accgctttgc ccgtcgccat cgtgcattag
     2041 gtttaggcgt gttgggctgg cacagctatc tacaggcaaa taatattgcc tttgacagct
     2101 tccaagcgat gcaaaaaaac aacgagattt tcaaaacgct tcaagcaaaa accttgaaag
     2161 cctcgcaaga attggctcaa cgcttcggcg agccggaaat tttagagggc tatggtcgcc
     2221 gtaatactac attaatgagt attgctccga ctaaatccag tagctttatt ttaggcagtg
     2281 tgtcgccatc ggtagagccg tttaaatcca actactatgt aaaagattta gcgaagatca
     2341 aaaccgtgta taaaaacccg ttcttagaga aattactcca agaaaaaggg ctagacaccg
     2401 aagaaatttg gacgtctatt ttacaaaatg atggctcggt tcagcatttg gaacagcttt
     2461 ccgagcacga aaaagaagta tttaaaacct ttgccgaaat cagccaatta agcgtgattc
     2521 agcaagctgc tcaacgtcaa aaatatattg accaagggca aagtattaat gtgatgatcc
     2581 acccagccac accggctcgt gatcttaacc aattatactt aacagcagaa gagctgggct
     2641 taaaatccat ctactaccaa aactcaatga gtgcagcaca ggtatttaac cgtaacctat
     2701 taagctgctc aagttgcgaa gggtaaataa tgggaaattt atcgcatact tcacctaata
     2761 ctattgttga agttgttggt gaatttcctg aaataacatc tagtgggtat acctttgata
     2821 aaggtcctat tcaattaaga atcggcaaac atactgctcc aaataaaggc tttggtgtaa
     2881 agcatattga agccgagcat atgcaagaaa ttcttaatct aggatttaca tcagttaatg
     2941 aatttgtcaa agctatcata gtagaaaacg ctaaaatttt ttgcgaattc tcagatttaa
     3001 aaggcaatca tcgccctatt attctgaaag caagtattgg cattgtggtt ctagagcgta
     3061 agtttttgga aagcggtacg atatattcag ttgtaaccgc ttatagaaga acaaatcctc
     3121 acggaataca aataggcact atgaaataaa gtacgttagt gttataatca gttccacgca
     3181 agagcatttg tagaaaataa caggcggatt tggttttcta ctgtgggcgg caggtttccg
     3241 ctaacctgta atagctattt aatttttata ttaaatcctg aacttgaaac agactaaggg
     3301 attgctgcgg tagctaagaa tacaaacctc tctataatca tatagagagg ttttttctta
     3361 ttgatgagca caagttaaga taattattta ctctcccgac ataacttcaa ggtagcttga
     3421 atcagctcaa aagctattgt gccttgttgt gggatttcag gtaagctttc ctcacagtcg
     3481 aaccattttg catcgcagat ttcttcttcc tgtagctgaa tttcaccgct ttcataatcc
     3541 gctaaaaaac ctagcattaa tgaattggga aacgcccaag gttggcttcc aaaataacgc
     3601 acatttttca ctttaatgcc cgtttcttca aacacttcac gatgcaccgc agtttctacc
     3661 gcctcaccgg cttcgacaaa accggctaaa gtggtataaa ttgtgccttt atggcgtaga
     3721 tgattggcta gtaggatttg tctgcctttt cgcaccgcca caataattga cgggctaatc
     3781 gttgggtaag ctcgattgcc acacgcattg cagtaaattg caatttcatc gcttgccagt
     3841 atggttttct cgccacattt tccgcaaaat tggtgagtct gaaagaaatg gttaagcgaa
     3901 acagctcgat gcaacaaatg agacagttcg ggtggtcggt taatttggct tcttaaataa
     3961 gcaaactctc gctgattatc cgattctaac gccttcacca aataagccgg cgagccttga
     4021 tattgcccga tggttaattt ctcacgtcca cttaagccca gctcttccgc acttccgaat
     4081 ggaatttccc cagcctccaa ggcaatatca aaatcgctga cgataatcca atatccttta
     4141 ttcatctttc aatcctaatt gtgtaaacaa cttacgtttt tcattttcag atagcacttg
     4201 aaactcacca cagcctaaat tgcctaatct aaactggcct atcccaacac gaattaaccg
     4261 taaagtcggg aaaccgatat gtgcagtcat tcttctgact tggcgatttt tcccttcaga
     4321 aataatcaat tctatccagc tggtcggaat ggttttccgc tcacgaattt gcggggcgat
     4381 attccaagca aaatcgggcg ttgctattgc tctgactttc gcgggcttgg tcaaaccatc
     4441 ttttaactcc accccttttg ctaatttggc aagatcttcc ggttgtggaa caccctccac
     4501 ttgtacccaa taggttttct gcttgtcaaa tttcgggtga gctaaacggt gctggatttc
     4561 gccattatta gtcaaaagca acaatccttc actgtctcta tctagccgcc cgaccgggta
     4621 aatattgggc atcgtaataa aatcttttag cgtttttcgc ccctgttcat cggtaaattg
     4681 ggttagtaca tcataaggct tattgagtaa cacaagggtg gtttccgcaa aggtaagcgg
     4741 tcgtttttgc ggattttttt gcaaattttt agcgaaattt gaccgcttgt ttgtcattct
     4801 tttaaaggtt ggttgtttca ttttttcagg ttcgcccacg caacagaaag taaaataatt
     4861 aagccaccga taatcatttt aagggttggc acttcattaa agcaaagcca cgcgatcaca
     4921 atcgcataca ccggctcgag ggaaataatc attgaggcag tacgggcgtt gatggtatca
     4981 aggcttgaaa taaacagcgt ataggcaagg gttgtacata aaaaaccgat acagaaaatc
     5041 caaaaccaat cttgggtgga aacgcttgga aattcagtga tggagagagg caacagaaaa
     5101 agtgtagcaa aaatatactg ccaccagctt gcttgcacac cggaaatttt gctcatggta
     5161 cggcgattcg ctaccgccaa aataccatac accaaagcag ataacacgcc ccacaataaa
     5221 ccttgagtgg cttgattacc aaaagtaaat tcaggggtaa ctaaaattaa accaaccgtg
     5281 atggcaatta atagcaccac ttcacgaaag cgtaattttt cacggaagaa caggctttca
     5341 aataacgcca caaatgccgg aaagctggca aagcctaagg ttgctaccgc cacaccacct
     5401 actttaaccg ccacaaaaaa agtaacccag tgggcagcta ataaagctgc agaaaatgca
     5461 agttgagcca tttgctgtaa gttgagcttt acaagcggtt gttttttcca taaaaaatac
     5521 aaagagatga tagcaaaagc aatcatcact cgcccaagca caatggtttc agccgagctt
     5581 tgaattaatg ctccgaagat ccccgaggca ccaaataaaa ccgctgtgcc gtgtacttta
     5641 agtagtgcaa gtgttctttt ctccactatt tgatcctatt atgttgatct tgctcgtccg
     5701 gcttgcgttc aaattctgca tcaaacgtgt tgctatcttg cgcagatgta acagaaaaag
     5761 acgaagtttg ggcggtaaaa tgcataaaca ccttattctt tacgctatta attacgaagg
     5821 cttgaatcca ttttcggcta aagggtaaca cgcataaaat cgctaaaata tcacttaaaa
     5881 agccgggaat aatcagcaaa atgccggcta caataaacat caccgagttg ccgactgctt
     5941 cagtcggtat cttgccttgg cttaaatctt gcttgatgga aaagagggta taaacgcccc
     6001 ttaacttcac aatccataac cctaaaaatg aaatcacgac cagtaacaaa atcgtgggta
     6061 acacgccaat gatcgagcca atattcacca gcagcgaaat ttcgcaatag atataaaaga
     6121 aaataccaaa cagaatcgct aaaataggca tattatcctc ccaacgttgc ggcagcgttt
     6181 gccgtcagat tttctgcccc attggctcgc atgacaatat tgtcttcggt acgaatcccg
     6241 ccaaaggctt tgaaagcctc aattttctgc cagttaaagg cagaagataa ggcactgttt
     6301 ttccaaggct taagtagcat ctcaataaaa taaaaacccg gctcaatggt aagcaccata
     6361 ttttcctgta gctcacgggt acaacgcaaa cttgggtaaa cctctggagg agctttatgg
     6421 ctgcctcggg cattttgctg aaaaccgccc acatcgtgca cttgaatacc caataaatgc
     6481 cctagtccgt gtgggaagaa cgtacggcta atcccttgtt caaaaatttg ctcggctgaa
     6541 agatgaataa attgatgttc cgctaataat tgcgaaatca tctgctgcat tcgggtgtga
     6601 taatgcaggt agttcacccc aatactcagc tcagcaataa tctgcaactt caactgttcc
     6661 attttcgcca ccagctcggc aaattcagaa ccgctgtcaa aggcataggt gcgggtgaga
     6721 tcggaggcat aaccatttac cgttgtgccg gcatcaatta aaaaactgtg gcgttttgcg
     6781 tgggtgtcaa aatctaacgc cgtataatgt agcaccgcac cattttgatt aatcgccaca
     6841 atattgccat aaggaacatt gagatcagat tgctgcgtcg ctttcaaata ttcaaggttg
     6901 atttcaaatt cgcttttgcc ggcaaaaaat gcctcttttg ctacttgatg cccttttaag
     6961 gctgaaaatt gggcttggta aatactttca atttcaaact ccgttttgat ggagcgggca
     7021 aaattcaggc tattcagcac tttctgcgga ttaatatggc taaagcctaa ctttttggct
     7081 agctcaacat cttcaccgat aaaggcacaa gcggtcggat tttggataaa ggttgcaatt
     7141 tcattctctg ctctcaacaa aacccactca aattccgcag cgaaaaaggc ctccgttggc
     7201 acttttagag tggaaaccca ataatcttca ggggcataaa aatagatttt cggcttattt
     7261 ttgccatcta aaaataacca gcaattttca gcttggggat atgggaaaaa gtaattaaaa
     7321 taagggttaa ttttaaacgg ggcggtttga tcgtctaaaa ataaggtttt tgccttgccg
     7381 gcataaatcc ataaaccgtc taaaaaggca ttttccagca agctttgcac tgtctgttgc
     7441 aaacgtgcga gatgcgtaga gaatagattt ttcataagga ataaaatgac tcaattaact
     7501 gtaaataaag aattagatac cttggggcta cgttgcccag agccggtgat gctcacccgt
     7561 aaaacaatcc gtaatatgga agagggagaa attttgcata tcatcgccga tgacccggcc
     7621 actacccgtg atattccgag tttctgcgaa tttatggatc accaattaat tcaaaaagag
     7681 gtggactcca ccccatacca ttactggatt aaaaaaggct tggaataaac cacaagcggt
     7741 cgattttagc cataaaattg caaatttatg cacaaaatcg accgcttgtt ttaattaatg
     7801 acggcgaggt tcgtcctcaa aatcgtcatt ttcatcatcg aaaaattcac cgtcatcgcc
     7861 gtactcatct tcatcagcat tcggatcttc aaagtaggtt ccccagccct catactccgc
     7921 accggctttt tcaattaatg gcagtaactc tttttgttgt gcggtaatga gatccgcatt
     7981 caagtgaatt tcgctcacaa tgtcgcatac aaaaatcact ttaccatttt cgtcttccac
     8041 ttcttccgct tcggaaattt catagcccag cttataggca tcaactacta atttttccaa
     8101 tttatcaaaa tcgtggtgag caacgtggtg ttcaataata taaagggctt cagggtctga
     8161 accatcggct aataatgatg aaatggtttc atcggtttct gtgcgtaatt catcgagatt
     8221 gtacatattg aattccttac ttattctgtg attaattttg gtgcaaacca gctatctaat
     8281 ttgttcgaca gcttatcaat accgatttta ttcagtgcgg aataagcctc tacttggata
     8341 tcaccttgga atggtaaaat cgcttctctt accattttta cctgcttaga gcgtgccgat
     8401 tggcttaact tatccgcttt ggtcaacagc agtaacaccg gcaagttggc tgacactgcc
     8461 cattcaatca tctgttgatc taaatctttc aacgggtggc gaatatccat taaaatcacc
     8521 acaccgccta aacattcacg cttttgtaaa tactcgccta atgctttttg ccactggatt
     8581 ttcatctgtt caggtactgc tgcatagcca tagccaggta aatccactaa tttacaattc
     8641 gtttcaacct taaacaagtt aatcaactgc gttcgccccg gtgttttgga agttcgggct
     8701 aaactttttt ggttagttaa agcgttcaaa gcagtcgatt taccggcatt tgaacgcccg
     8761 gcaaaagcaa tctccacacc tgtatcttca ggtaaatgac gaatatccgg tgctgaagtt
     8821 aaaaaatggg ttttatgata gtttaatttc attttattct ctaaatttaa aagataagcc
     8881 ttgctcgcca tattgattcg gttcaggatt accggcaaat acaccccatt ctaaaaagag
     8941 caacattcca ataaatgccg gcatcacctg tccaagcaat atctgcataa ttccttgtga
     9001 ataaggagct gcaagataac aaataatcgg cactaatgcc attaacaggg cttttgccga
     9061 acgatttcgg tcgtgtaatc gtttaataat aatagcagac aggctaaatc ctgtaattat
     9121 aagcggtagc acactcaata aagacaattc agccaaattt atccacatag caaccataaa
     9181 caggaaaaga aagtggattc caaatccaat ccaaaaacct cgtctattta gcctaccgtt
     9241 aaaactaaaa agtgctttat accaactcat tttctaccct aaagctacat caagtaccat
     9301 cattactaca aagcctaaca tcaggcttaa agtcgcaata tcagtatttc ccgaactttg
     9361 cgattccgga atcagttctt ctacgaccac aaaaatcatc gctccggcag caaaggcaag
     9421 agcataaggt aaaatagccg tcatcgacag cacaaaaacc gccccaatca ccgccgcaat
     9481 aggctcaacc actgctgaca tagagccata ccaaaatgct ttcttccgac tatgcccttc
     9541 gcctcgaatt ggcaatacaa gtgacgagcc ttcgggaatg ttttgtaacc ctattccgat
     9601 tgccaaaccg atagctccca taatggaagc atctacgcct gaaacttggg ttgccaaagc
     9661 accgaaagcc acccctatgg ctaatccttc tggaatatta tgaatggtaa tcgcaagaaa
     9721 aagcaaagta gattttgata agccttgttt aagttgatca tttccttcag cttgctcaat
     9781 cggcttactc aagtgcaaat gcggcacaac atagtcaatt aatcgaataa aagctccacc
     9841 ggcaagaaag ccaatcgctg caggcagcca agctaaagag ccataatccg cctcggcata
     9901 ttctaaagaa ggtgcaagca aagaccaaaa cgaggctgca atcattacac ctgcggcaaa
     9961 gcccatcata ctgtctaata attttcgatt aaccgattta aaaaaataga caaatgacga
    10021 accgaaaatc gtacaacccc aagtaaataa acccgctaaa aaagcttgcc atattggaga
    10081 aagtgaaagg aaatattcaa acaagaaata gtaccagcta aaataaatat tggctcattc
    10141 tatcaaagat tcagggttat taatatagtg tagccaaagg ctcaacgaag atttcacttg
    10201 taaaacttag caagaaaata accgcttgca tttccctttc ataaatacaa tgaaaatcct
    10261 aacaagcggt cattttccgt gagtttttta cctttaccac aacaacaaaa aaccgcctgt
    10321 atctctacag gcggctcttt cttggattac ttgtactttg tttatttgta cttattttgc
    10381 ttttgcttaa gttttcgctt ttgtaacttt tagctttcgc ttaagctcgc ttaaattaaa
    10441 acccgacgat gctctactct cacatggcga atcaccacac taccatcgac gttactgcgt
    10501 tttacttctg agttcggtat ggggtcaggt aggaccacag cactcttgtc gtcgggaaaa
    10561 ttcttttcta tttttgaatt tctttaagtt aacttcctaa tctaactctt agttcacttt
    10621 aatagttaga ttaacttcct taacttcgat tgatttcttc tctagtttcg ttcttcttct
    10681 caatacaaaa acaagcctaa aaacatttga gcgttgtata gttaagcctc tcgggcaatt
    10741 agtactggtt agctcaatgt ctcacaacac ttacacaccc agcctatcta cgtcgtagtc
    10801 tccaacaacc cttacacact tatagtgtgg gagaactcat ctcgaggcaa gtttcgtgct
    10861 tagatgcttt cagcacttat ctcttccgca tgtagctacc cagcaatgcc tctggcgaga
    10921 caactggaac accagtgatg cgtccactcc ggtcctctcg tactaggagc agcccctctc
    10981 aattctccaa cgcccacggc agatagggac cgaactgtct cacgacgttc taaacccagc
    11041 tcgcgtacca ctttaaatgg cgaacagcca tacccttggg acctacttca gccccaggat
    11101 gtgatgagcc gacatcgagg tgccaaacac cgccgtcgat atgaactctt gggcggtatc
    11161 agcctgttat ccccggagta ccttttatcc gttgagcgat ggcccttcca ttcagaacca
    11221 ccggatcact atgacctgct ttcgcacctg ctcgacttgt ctgtctcgca gttaagcttg
    11281 cttataccat tgcactaacc tcacgatgtc cgaccgtgat tagcaaacct tcgtgctcct
    11341 ccgttactct ttgggaggag accgccccag tcaaactacc caccagacac tgtccgagac
    11401 cgcgttccgc aatcttcgtt agaacatcaa acgttaaagg gtggtatttc aaggacgcct
    11461 ccacaatcac tggcgtgact gcttcaaagg ctcccaccta tcctacacat caaaattcaa
    11521 tgttcagtgt caagctatag taaaggttca cggggtcttt ccgtctagcc gcgggtacac
    11581 cgcatcttca cggcgatttc aatttcactg agtctcgggt ggagacagcc tggccatcat
    11641 tatgccattc gtgcaggtcg gaacttaccc gacaaggaat ttcgctacct taggaccgtt
    11701 atagttacgg ccgccgttta ctggggcttc gatcaggagc ttctcttgcg ataacaccat
    11761 caattaacct tccagcaccg ggcaggcatc acaccctata cgtccacttt catgtttgca
    11821 gagtgctgtg tttttaataa acagttgcag ccagctggta tcttcgaccg gttcaacctt
    11881 catccgcaag ggactacaat ctacgccggc gcaccttctc ccgaagttac ggtgctattt
    11941 tgcctagttc cttcacccga gttctctcaa gcgcctgagt attctctacc tgaccacctg
    12001 tgtcggttta tagtacggtt tataataacc tgaagcttag tggcttttcc tggaagcgtg
    12061 gtatcgatta cttcatctcc gtagagactc gtcatcactt ctcggtgtta aggaagaccg
    12121 gatttgccta atcttcccac ctaccggctt aaacagacat ccaacagtct gctaacctaa
    12181 ccttctccgt ccccacatcg cagttattac aagtacggga atattaaccc gtttcccatc
    12241 gactacgctt ttcagcctcg ccttaggggc cgactcaccc tgccccgatt aacgttggac
    12301 aggaaccctt ggtcttccgg cgaacgagtt tttcactcgt tttatcgtta cttatgtcag
    12361 cattcgcact tgtgatatct ccagcagact tctcaatcca ccttcatcga cttacacaac
    12421 gctcccctac ccaacagact ttcgtctgat gccgcagctt cggtgctata tttgagcccc
    12481 gttacatctt ccgcgcaggc cgactcgact agtgagctat tacgctttct ttaaatggtg
    12541 gctgcttcta agccaacatc ctagctgtct aagccttccc acttcgtttc ccacttaata
    12601 tagactttgg gaccttagct ggcggtctgg gttgtttccc tctccacgat ggacgttagc
    12661 acccaccgtg tgtctcctga gtatcactct tcggtattcg cagtttgcat cgggttggta
    12721 atccgggatg gacccctagc cgaaacagtg ctctaccccc gaaggtgtcc gctcaaggct
    12781 ctacctaaat agatttcggg gagaaccagc tatctcccgg tttgattggc ctttcacccc
    12841 cagccacaag tcatccgcta atttttcaac attagtcggt tcggtcctcc agttagtgtt
    12901 acccaacctt caacctgccc atggctagat caccgggttt cgggtctata ccttgcaact
    12961 acacgcccag ttaagactcg gtttccctac ggctccctta ttcagttaac cttgctacaa
    13021 aatataagtc gctgacccat tatacaaaag gtacgcagtc acaagattac tcttgctccc
    13081 actgcttgta cgcacaaggt ttcaggttct atttcactcc cctcgccggg gttcttttca
    13141 cctttccttc acagtactgg ttcactatcg gtcaatcagg agtatttagc cttggaggat
    13201 ggtcccccca tcttcaaaca ggatatcacg tgtcccgccc tacttgttgt tagcctagta
    13261 ccacaatgga gttttaaggt acgggattat caccctctac gattgtcctt cccagaacat
    13321 tcccctaact ttactgctat cactaactgg ctctttcgcg ttcgctcgcc gctactaacg
    13381 aaatctcggt tgatttcttt tcctcggggt acttagatgt ttcagttctc ccggtttgcc
    13441 tcacacagct atgtattcac tgggtgatag taggttcttc acctactggg tttccccatt
    13501 cggacatctt ggattaaacg cctcttatcg actcatccaa gcttttcgca gattagcacg
    13561 tccttcttcg cctctgattg ccaaggcatc caccttgtgc gcttagtcac ttaactatac
    13621 aacctcaaat gttttctaaa acttctcaaa aataaaagta aaaaaacaat gaagtcagtt
    13681 ttacaactaa acacttgact gcttttgttc agtcaagatt ttttctactc agactttcaa
    13741 tttattcgtt atcacttatg tataattact tatgcgtaac gaacttgaaa tatctctcag
    13801 tttttcagct tgtttctata ttgttaaaga acagaagaca ataaaaatca tctttaaatg
    13861 gcgtccccac ggggattcga accccggtta ccgccgtgaa agggcgatgt cctaggcctc
    13921 tagacgatgg ggacaacatt taaagatgct ttccactttg ccatttagga gtaaaatatt
    13981 ctagccaatt ttgcaaaaat cttcaagatt ttcaccgctt ggatggctat ctttcactca
    14041 tcttctctca ttcat
//
LOCUS       DANLXS010000057        13839 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.057, whole genome shotgun sequence.
ACCESSION   DANLXS010000057 DANLXS010000000
VERSION     DANLXS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13839
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.057"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..99)
                     /locus_tag="PXD71_001204"
     CDS             complement(<1..99)
                     /locus_tag="PXD71_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL6291333.1"
                     /translation="MRKIKLLHVILSLILGLSAAAIGSYPAIKEIGE"
     gene            182..1240
                     /locus_tag="PXD71_001205"
     CDS             182..1240
                     /locus_tag="PXD71_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6291334.1"
                     /translation="MAEILFRSCLCCNAKRIFYLLTFYKGFETSQIATAFSLLVFTSQ
                     AFSLFAGSWGDSFGRYPIMMLGCLLDALAYILLLTTKNYPLLLLATFFFGLGSTLFST
                     NARAFLLANIQDTGDSYQLKTKAQGKFLKVSSLSAMVAPLLTIPFIFYRKAEWLIWIS
                     CCIEIIMLFVMFKTAPKTHECANGQCERFKFGALKGILNEQFIFVHLLLFIPLGLGSA
                     FYIIFPYIFTNLLDKPELVSVSFFVNNLIAVLLQSDFSRKLNLTVHKLIYIAPILIIL
                     QIMPWFYALQWLSTITAFLYLVIFATITVYANTALSNMLVKLDNGKNQGLMFGSSKLI
                     LAITTLGVMNVLPYIFLI"
     gene            complement(1237..2370)
                     /locus_tag="PXD71_001206"
     CDS             complement(1237..2370)
                     /locus_tag="PXD71_001206"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDL6291335.1"
                     /translation="MLIFMMNEYSPNYYIGVMSGTSLDGVDIALLDFAKNPPKMTACD
                     FFPMPEELRADISALLKTGETNLQKLGEIDHRLGLLYAETINAFLAKNQLKAEDIQAI
                     GCHGQTVWHSPDSEYPFTTQIGDMNLVAEQTGITTIADFRRKDMAVGGQGAPLVPAFH
                     QAIFSDPNRLTVVLNIGGISNISVLNPNSPTVGYDVGAGNTLMDSWIEQHQGKRYDKN
                     GEWARTGKLNTELLATLLDESFFAKAPPKSTGRELFNLTWLAKKLENLTACQPQDVQR
                     TLAEFTAQSIANELLRFESSLPRLLLVCGGGSRNPLLMERISALLPEWEVTTTTEFGL
                     DIDYVEAAAFAWLAYQRIHNLPSNMPSVTGAKKAVSLGVIYPA"
     gene            complement(2562..3401)
                     /gene="queF"
                     /locus_tag="PXD71_001207"
     CDS             complement(2562..3401)
                     /gene="queF"
                     /locus_tag="PXD71_001207"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDL6291336.1"
                     /translation="MNYNDKSLSALKLGQKTEYKSEYDASLLQPVPRKLNRDSLGITQ
                     EQPFNRGADVWTCYELSWLNQNGLPQVAIADVAIDFKSENLIESKSFKLYLNSFNQTK
                     FSSIEEVGKTIQADLARCASGQVSVKIYQLSHYTQQPIVDFAGDCIDEQDIQIDSYAF
                     STHYLENIAEDELVEETLVSHLLKSNCLITSQPDWGSVQIRYKGKKLNREKLLRYLVS
                     FREHNEFHEQCVERIFCDLMQFAEPEKLTVYARYTRRGGLDINPFRSNFEEVPENLRM
                     ARQ"
     gene            complement(3406..3954)
                     /gene="gmhB"
                     /locus_tag="PXD71_001208"
     CDS             complement(3406..3954)
                     /gene="gmhB"
                     /locus_tag="PXD71_001208"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDL6291337.1"
                     /translation="MGKKAIFLDRDGTINIDHGYVHQIDDFQFIDGVGKALKQLQDKG
                     YLLVLVTNQSGIARGYFSEAQFMQLTEWMDWSLDEDYGVVLDGIYYCPHHPEGKGEYR
                     KDCDCRKPKAGMFTQAIQDLNIDPSQSYMVGDKLEDILAAEAAGVKTKVLVRTDKAVT
                     EEGQAKADLVLDSLVDLVKYIK"
     gene            complement(3954..4421)
                     /gene="bcp"
                     /locus_tag="PXD71_001209"
     CDS             complement(3954..4421)
                     /gene="bcp"
                     /locus_tag="PXD71_001209"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDL6291338.1"
                     /translation="MNILQAGDKAPKFTLQNQADEPVSLSQFAGKKVLVYFYPKALTP
                     GCTTQACGLRDSKSELDELNVVVLGISTDLPKKLAQFVEKKALNFTLLSDPDHQVAEA
                     FGVWGEKKFMGKTYDGIHRISFLIDEQGGGEQVFDKFKTGEHHQMIVDYLRGR"
     gene            complement(4508..4705)
                     /locus_tag="PXD71_001210"
     CDS             complement(4508..4705)
                     /locus_tag="PXD71_001210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291339.1"
                     /translation="MKFGYLNGVLEGKGKMRFDEAKLHAESEFEKYRIVQDRLFESDF
                     DKVLKEMLRSENQYRPSFSTT"
     gene            complement(4674..5156)
                     /locus_tag="PXD71_001211"
     CDS             complement(4674..5156)
                     /locus_tag="PXD71_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL6291340.1"
                     /translation="MIDKQIRNSTAEFLIFTQQNGAENITVRYEDNEIWLTQKLIAQL
                     FDCSTDNVSLHLKNIFNSRELDPNSVTEDFSVTAADVKNYRTKHYNFDAVISIGYRVN
                     SVRATQFRQWATKVLRNFAQKGYVIDRQRMENGTFLNEDYFEQLLAEIREIWLSERRS
                     "
     gene            complement(5299..6456)
                     /gene="hemW"
                     /locus_tag="PXD71_001212"
     CDS             complement(5299..6456)
                     /gene="hemW"
                     /locus_tag="PXD71_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDL6291341.1"
                     /translation="MNLILPPLSLYIHIPWCVQKCPYCDFNSHAQKGLIPEQEYIEHL
                     LADLSQDLTAYRVAIGDRKLYSIFIGGGTPSLFSAEGIAYLLIEVEKRIAFEPNIEIT
                     LEANPGTAEAERFLGYAQGGVTRISMGIQSFEPEKLLKLGRIHDANEAKQAIKFAQNS
                     AKSGLQSFNIDLMHGLPNQSVKQALADLETGISLNPPHLSWYQLTIEPNTMFYYRKPT
                     LPDDDELWDIFEQGHQLLTNAGYEQYETSAYAKKGYQCRHNLNYWRFGDYLAIGCGAH
                     GKISYPTGEIYRFSKIKHPKGYMRGEYRYSQDLIELDDRPFEFFMNRFRLLEATPKQE
                     FEWFTGLDREVVRPTIDWALNQNYISETATHWQITQHGKLFLNELLEGFLE"
     gene            6678..8261
                     /locus_tag="PXD71_001213"
     CDS             6678..8261
                     /locus_tag="PXD71_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6291342.1"
                     /translation="MKKQFEHNESRRGFMKLIAGVGAGMAFSGTLGTFTPKAFAAPAA
                     GSTIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLVDLHPATRKPYLALAAAEPEK
                     IDDVTYRITLRDGAKFHNGNPVTTEDVVYSFERVLDPAKASLFAQFIPFIDTVKKVDD
                     KVVEFKLKYPFALFKERLTIVKIVPKAVVEAGQAAFDANPVGTGPYKFVSATKDDRIV
                     FEAFADYNGGYPAQVEKMTWFLLSDDAARVTAQESGRVQAIESVPYLDAERLKRKGTV
                     ESVQSFGLLFLMFNCEKAPFNNPKVRQALHYGLDTQKLIDVVFLGNAKAATSYVQDTH
                     PDYVKANSQYDFDKAKAEALLAEAGVTELKFELLATDHSWVKECAPLILESWNSLKGV
                     KVSLKHLQSGALYGTHVDKGAYEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRW
                     SETAEYAEVQKLLDEAAKNPAASKEAWAKAINIIAEQVPLYPIIHRKLPTAWNDKALT
                     GFQPLPTTGMSFIGVGRAK"
     gene            8473..9426
                     /locus_tag="PXD71_001214"
     CDS             8473..9426
                     /locus_tag="PXD71_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6291343.1"
                     /translation="MEIILRLLWRRLMALPVMILGVTALVFIVLQFTPGDPATVALGE
                     SASEAAKEIYREQHGLNDPLLVQYFRFLGNLFVLDFGMTTPPEQPIVDMIAKAFPLTL
                     QLTFIGVFLAAIVSFTLGVTAALYRDTWIDQLIRLISVAAVATPSFWLGILLIQYFSL
                     KLDWLPSGGFIPFSEDPKGYVNSMVLPSLALAVPVCASLIRVVRTTMVEEMDKDYVRT
                     AIGNGVPYSTVIRHNVLRNALITPVTVLGLRVGYLLGGAVVIEQIFDLPGMGKLIFNG
                     IVNHDLNLVQGVVLTIAFTFVLVNIIVDILYLLINPKIRSL"
     gene            9428..11398
                     /locus_tag="PXD71_001215"
     CDS             9428..11398
                     /locus_tag="PXD71_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDL6291344.1"
                     /translation="MFRQGLAARLASGGARFRALSTSSKIALLFIVFIALVAIFAPLV
                     APYDPLQTLRPVQAPSGDYLFGTDRLGRDIFSRMVWGARTSLFIGLGAVGVAILFGSI
                     LGATAATADKFGNEVIMRFMDILMAFPGIALAAVLLATFGNSVPVIIVTIAVVYTPQL
                     ARVVRANVVSQWEEDYVRAERVIGGSRTYILLKHVVRNTAAPVLVFATVMVADAIVFE
                     ASLSFLGAGVQPPFPSWGNILSEGRNLVLSGFWWATTFAGIMILLTVLALNILAEGLT
                     DALVNPKLKRSTQSKEDVAKPLATDVQEAMSETLALKRYLLKLHEKETTRTDRMQLNS
                     TAKPILQVKNLSIRFPNRYGETPLVDNISFTVHEGETMGLVGESGCGKSITAFSIMGL
                     LPKTAKITGEILFTDRSGKQHNLLESADLNALRGHEISMIYQDALSALNPSMRIKDQM
                     AQLISRGGKQSAETLLQWVKLDPEKTLNRYPHELSGGQRQRVLIAMALAREPKLLIAD
                     EPTTALDVTVQAEVIKLLNELREKLGFAMVFVSHDLALVAQIAHHITVMYAGQVVETA
                     PTTPLLANPTHEYTRGLLGSVLSTELRAERLYQIPGSVPSPFDFAKGDRFASRSLRPD
                     ANPEQHLKLVPTGDHPQHFWASHLEDPQSKEA"
     gene            11413..12219
                     /locus_tag="PXD71_001216"
     CDS             11413..12219
                     /locus_tag="PXD71_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6291345.1"
                     /translation="MSMKFIKEQPIIELSNIVVQFASRDGTLFNPKKFTAVNDVSLAI
                     HAGETVGLVGQSGCGKSTLASVMIGLQKPTSGTVKFNGLEMKYGSAEARKYFGSQVSV
                     IFQDPATALNPRMRVLDILKDPMDIHNILQPQEREKRVYDLLSRVGLPRSAALVEPTR
                     LSGGQKQRVAIARALALNPKLIVADEPTSALDVSVRAQVLNLLADLKKELNLAMVFIS
                     HDLQTVHQVSDRIVVMNGGKIVEMGDASDVFERPTQEYTRTLIEAAPSLL"
     gene            12282..>13839
                     /locus_tag="PXD71_001217"
     CDS             12282..>13839
                     /locus_tag="PXD71_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDL6291346.1"
                     /translation="MIQITQTINIDLLIVGSGIAGLSAAVEAKEKGINTLLISKSTIG
                     SGASYFPLKATLGIQVTGDEADKIHFKEDIENIGKGKINPNVVQAYIEDSPQAIELLH
                     RIGFQPWKRNDNRPACFAKYPRPIYLIKDWRESALRAKQILQEQHIAFYENATLLHIV
                     TDNNQVQGAVFSLTTSGQISYIFCKTKHLILAAGGIAGLYKDNLYPADVIGSLHYVAQ
                     QAGATLVNLAFIQFIPAFVEPKYKVLFGEHTLKYVTQITDSKGNNLFPHLNAEQFAKM
                     VLERSHYAPFSLDFAGVEFDLVMMKHLLENPHEKGIYLKYSPKLYEDTEEFYTVYLNW
                     LKNEVGIDLVQDKIAIKPFAHSCNGGIQINQWGESAVKGLFAIGEISHCIEGANRMGG
                     NSVGGGLVFAKRAVSEIVRSLEQNDLQKNSEISPLAMAEKALNQLANSTANLELTASE
                     VLSTIREKMALYANVYRTRSNLKLLLEELNRLEQEFDPIYHAQYQGIEIYNALKTAQL
                     VVNQMLNEQSL"
BASE COUNT         3981 a         2946 c         2708 g         4204 t
ORIGIN      
        1 ctcgccaatc tcttttattg ccggataact tccaatcgct gccgcagaaa gccctaatat
       61 taaagataaa ataacatgca ataatttgat ttttctcatt gtgcttcctt attaaattta
      121 gttatattag aaatcatttt tctcattaaa tattatctat gaatatccaa aatatcattt
      181 tatggcggaa attcttttcc ggtcttgctt atgctgcaat gcaaagcgta ttttttatct
      241 acttacattc tacaaaggct ttgaaaccag ccaaattgca actgcatttt ctttgttggt
      301 ctttaccagc caagcatttt ccctttttgc aggttcttgg ggtgatagtt ttggacgtta
      361 ccctatcatg atgcttggct gtttgctcga tgccctagct tacattttat tactgacaac
      421 aaaaaattac cctctgctat tacttgctac ctttttcttc gggctcggta gcaccttatt
      481 tagtaccaat gcgagagcat tcttattagc caatattcaa gataccggag atagttacca
      541 actcaaaact aaggctcaag gtaaatttct gaaggtatca agcctgtcag cgatggtagc
      601 accattactt actattccat ttattttcta ccgtaaagca gaatggctta tttggattag
      661 ctgctgcatt gaaattatta tgctattcgt tatgtttaaa accgcaccca aaacacatga
      721 atgtgcaaat gggcaatgcg aacgctttaa atttggtgca ttaaaaggaa tactaaatga
      781 acaatttatt tttgtacatt tattactttt tattccgctt ggtctaggaa gtgcatttta
      841 tattatattc ccctatatat tcaccaatct tctggataaa ccagaattag tctctgtctc
      901 attctttgtg aataatttaa ttgcagtgtt attgcaatct gatttttcac gaaaattaaa
      961 tctgacagta cataaactta tttatattgc accaatactg attatacttc aaataatgcc
     1021 ttggttttat gcactccaat ggctttcaac catcactgca tttctctatt tagtgatttt
     1081 tgccactatt accgtgtatg cgaatacggc actatctaat atgctagtga aattagacaa
     1141 tggtaaaaac caaggcttaa tgtttggaag ctctaaatta attttagcta ttaccactct
     1201 cggagtaatg aatgtattac cttacatttt cttaatctaa gcagggtaaa tcacgcccaa
     1261 actcacggct ttttttgctc ctgtcacgct aggcatatta ctgggtaaat tatggattcg
     1321 ctggtaagct aaccaagcaa aagcggcagc ctctacataa tcaatatcta aaccaaattc
     1381 tgtagtggtt gtaacctccc actcgggaag taaagcagaa atccgttcca ttaataacgg
     1441 atttcttgac ccaccaccac aaactaaaag caagcgaggc agagagcttt caaagcgtaa
     1501 taactcattg gcaatacttt gagcagtaaa ttctgccagc gtgcgttgca catcttgtgg
     1561 ctgacaagcg gtcaaatttt ccaatttttt tgcaagccac gttaaattaa acagctcccg
     1621 cccagtgctt ttaggcgggg ctttagcaaa aaacgactcg tctaataacg tcgctaacag
     1681 ttcagtattg agcttgcccg ttctcgccca ttcgccgttt ttatcgtatc ttttgccttg
     1741 atgttgttcg atccaactat ccatcaaagt atttccggct cctacatcat aaccaactgt
     1801 aggggaattg ggatttaaca ccgaaatatt gctgatgccg ccaatattca gcaccactgt
     1861 tagacgattt ggatcgctaa aaatagcttg atgaaatgcc ggcacaagcg gagccccttg
     1921 cccacctact gccatatctt tacgccgaaa atcagcgata gtggtaatac cggtttgctc
     1981 tgccaccaaa ttcatatcgc caatttgggt ggtgaaggga tactcagaat ccggtgaatg
     2041 ccacaccgtc tgcccgtgac aacctatggc ttgaatatct tctgctttta gctgattttt
     2101 cgctaaaaac gcattgatcg tttctgcata aagcaagcct aaacggtgat caatttcccc
     2161 cagtttttgg agattggttt cgccggtttt aagtaacgca gaaatatccg cccgtaattc
     2221 ctccggcatt ggaaaaaaat cacaagcggt cattttcggt ggattttttg caaaatccag
     2281 caaagcaata tccaccccat caaggctcgt gccggacatt acgcctatat agtaatttgg
     2341 gctatattca ttcatcataa aaatcaacat ctgctatttg ttacttttct ttgctttgcc
     2401 aaagaaaagt aaccaaaaga aaggcaaccc gagcttcacc gctatcctcg cttagtttga
     2461 attggcttaa cggcaaattt acattcgctc cgctcagagt aaatttacct accaattcaa
     2521 acacgctcag gcggttcaga cgggaacaaa aaacaacaaa atcattggcg tgccattctt
     2581 agattttccg gcacttcctc aaaattcgat ctaaacggat tgatgtctag cccgcctcgt
     2641 ctggtgtagc gtgcgtaaac tgttaatttt tccggctcgg caaattgcat taaatcacag
     2701 aaaatgcgtt ccacacattg ttcgtggaac tcattatgct cacggaacga cactaaataa
     2761 cgtaacagtt tttcacggtt tagctttttg cctttgtagc gaatttgcac acttccccag
     2821 tcaggctgtg acgtaattaa gcagttagat ttgagcaaat ggctgactaa ggtttcttcc
     2881 accaactcat cttctgcaat gttctctaaa taatgagtcg aaaacgcata actgtcgatt
     2941 tgaatatctt gctcatcaat gcaatcgcct gcaaaatcaa ctatcggctg ttgcgtgtag
     3001 tgtgataatt ggtaaatttt taccgaaacc tgaccgcttg cacagcgagc cagatccgct
     3061 tgaatcgttt tcccaacttc ttctattgag ctaaatttgg tttggttaaa actgttcaaa
     3121 tagagcttga aacttttcga ttcaattaag ttttcgctct taaaatcaat ggcgacatct
     3181 gcaatcgcta cttgcggtag cccattttgg ttcaaccaag atagctcata gcaagtccac
     3241 acatcagccc ctcggttaaa aggctgctct tgtgtaatgc ctaaactatc acggtttaat
     3301 ttacgaggaa caggttgcaa aagcgaggca tcatattcgc ttttgtactc ggttttttgc
     3361 cctaatttca gggcggaaag ggatttatcg ttataattca ttgctttatt taatatattt
     3421 tactaaatca actaaactat ctaacaccaa atcggcttta gcctgaccct cttccgtcac
     3481 tgctttgtca gtgcgaacca gcactttggt tttcacgccg gcggcttctg cggcaaggat
     3541 atcttctaat ttatcgccca ccatataaga ttggcttggg tcgatgttta aatcttgaat
     3601 cgcttgagta aacatacctg ctttaggctt acggcaatca caatctttac ggtattcgcc
     3661 tttgccttcg gggtgatgtg ggcagtagta aataccgtct agtaccacgc cataatcttc
     3721 atcgagcgac cagtccatcc attcggttag ttgcataaat tgtgcttcgc taaaatagcc
     3781 tcgggcaatg ccagattgat tggttaccag caccaataaa taacctttgt cttgtagctg
     3841 ttttaacgcc ttgccgacac cgtcaataaa ttgaaaatca tcaatttgat gcacatagcc
     3901 gtgatcgata ttaattgtgc cgtcacggtc taaaaaaatg gctttttttc ccattagcga
     3961 ccccgtagat aatccacgat catttggtga tgttcgccgg ttttaaattt atcaaacact
     4021 tgctcacccc ccccctgctc atcaattaaa aaactgatgc gatgaatgcc gtcataggtt
     4081 tttcccataa atttcttttc gccccataca ccaaaggctt ctgcaacttg gtgatccggg
     4141 tcagataata aggtaaaatt aagtgctttt ttctctacaa attgagctaa ttttttcggt
     4201 aaatcggtgc taattccgag aacaaccaca ttgagctcat caagttctga tttgctgtca
     4261 cgcagtccgc acgcttgcgt agtgcaaccg ggtgtaagag ctttcggata aaaataaacc
     4321 agtacttttt tgccggcaaa ttgagagaga gaaaccggct cgtcagcctg attctgaagg
     4381 gtaaatttag gggctttatc gcctgcttgt aagatattca tttgtaattt ttccataaaa
     4441 aataaccgct tgcattatac acggcaagcg gtcaaacttg gcaaaaaatt tgcaaaatgc
     4501 ggttgtttta tgttgttgaa aatgatggac ggtattgatt ttccgaacgc aacatttctt
     4561 tcaatacctt gtcaaaatcg ctttcaaata atctgtcttg aacaatgcgg tatttctcaa
     4621 attcgctttc tgcgtgcaat ttcgcttcat caaaacgcat tttgcctttg ccctcaagaa
     4681 cgccgttcag ataaccaaat ttcacgaatt tctgccagca actgctcaaa ataatcttca
     4741 tttaaaaatg taccattttc cattctttgg cggtcaatca catagccttt ttgggcgaaa
     4801 ttccgtaata ctttagttgc ccattggcga aattgtgtcg cccgcaccga gtttacccta
     4861 tagcctatcg aaataacggc atcaaagttg tagtgtttgg tacgataatt tttgacatct
     4921 gcggcagtta ccgagaaatc ctcggtaact gaatttggat cgagttccct actattaaaa
     4981 atgtttttta aatgcaaaga aacattatct gttgagcaat caaaaagctg agcaattaat
     5041 ttttgcgtaa gccaaatttc gttatcttca tagcgaacgg taatattttc tgccccattt
     5101 tgttgagtaa aaatcaaaaa ttcggcggta ctattacgga tttgtttatc gatcataagc
     5161 tctccttatt gtgaaggcta tgctactaaa cactgttttt ttgtctagta aaaaatgagg
     5221 atttttcgac gcagatcaca aaaaacgcct gtaatttagc attacaatgc ggtctttttt
     5281 tagtcgtttt tttgcaaatt attccaaaaa tccttccagt aattcattta aaaacaattt
     5341 accgtgttgg gtaatttgcc aatgggttgc ggtttcggaa atatagtttt ggtttaacgc
     5401 ccaatcgatt gtcgggcgaa caacttccct gtctaaccct gtaaaccatt caaattcttg
     5461 ttttggtgtc gcttcaagta atctaaagcg gttcataaaa aactcaaacg ggcggtcgtc
     5521 taattcaatc aaatcttggc tgtagcgata ttcacctcgc atataacctt tcgggtgttt
     5581 gattttgcta aagcgataaa tttccccggt cggatatgaa attttgccgt gagcgccaca
     5641 accaatcgcc aaataatccc caaatcgcca ataatttaaa ttgtgtctgc attggtagcc
     5701 ttttttggca taagccgagg tttcatactg ctcatagccg gcattcgtta aaagttgatg
     5761 cccttgctcg aaaatatccc ataactcgtc atcatcaggc aaggttggct tacgataata
     5821 gaacatcgta ttcggctcaa tcgtgagctg ataccacgat aaatgcggtg gattgagtga
     5881 aatccctgtt tctaaatccg ccaaggcttg tttaaccgat tggtttggta agccgtgcat
     5941 taaatcaatg ttgaaacttt gcaagcccga ttttgcagaa ttttgggcaa atttaatcgc
     6001 ttgttttgct tcatttgcat cgtgaatccg tcctaatttc aataattttt ccggctcaaa
     6061 actttggatt cccattgaaa tgcgggttac gccaccttgt gcgtaaccta aaaatcgctc
     6121 tgcctctgcg gttcccgggt tcgcttccag tgtaatttcg atatttggct cgaatgcaat
     6181 ccgtttttct acttcaatta ataaataagc tatcccttcc gctgaaaata agctcggggt
     6241 gccgccgcca ataaaaatag aatataattt acgatcacct attgccactc ggtaagcggt
     6301 caaatcttgg gataaatctg caagtaaatg ttcgatatat tcttgctcgg gaattaatcc
     6361 tttttgagcg tgcgagttaa aatcgcaata agggcatttt tgcacgcacc aagggatatg
     6421 gatataaagg cttaaaggcg gtaaaatcag gttcactgtc aatttttcat ttatgattaa
     6481 attggcgaat aataacgaaa agcacgtgaa gtgaaaagca aagattttcg tgagctagat
     6541 cacaaattca ataattcttg ctttaccact ttgacattaa tgatatgata aattcaattc
     6601 aaaaaagtgc attttttgat aaattcactt cagcaaataa taccgcccct atcataaaaa
     6661 cttaaaggag ctttaagatg aaaaaacagt ttgaacacaa cgaatcccgc cgtggtttta
     6721 tgaaactaat tgcaggtgtc ggtgccggta tggcatttag tggcacttta ggtacattta
     6781 cacctaaagc atttgcagcc cctgccgccg gctcaacgat tgaggccggt atagcttatc
     6841 ccatatcaac cggatttgac ccgcttacct caagcggtgc atcctcaatg gcggcaaacc
     6901 tccatatttt tgaaggcctc gtggacttac atccagccac tcgcaaacca tatttagcat
     6961 tggcagcagc agagcctgaa aaaattgatg atgtaaccta tcgaattact ttacgtgacg
     7021 gggctaaatt ccacaatggt aatccggtta ccactgaaga cgtagtttac tcatttgaac
     7081 gtgtattaga cccagctaaa gcatcacttt tcgcacaatt cattccattt attgacactg
     7141 tgaaaaaagt tgatgacaaa gtggttgaat tcaaattgaa atatccattt gcgttattca
     7201 aagagcgcct aaccattgtt aaaatcgtac caaaagcagt ggtagaagcc ggtcaagctg
     7261 catttgatgc taatccagtg gggactggcc catataaatt tgtatccgca actaaagatg
     7321 atcgtattgt tttcgaagca tttgcagatt ataacggtgg ctacccggca caagtcgaga
     7381 aaatgacttg gttcttactt tctgatgatg ccgctcgtgt aaccgctcaa gaatctggcc
     7441 gtgttcaagc aattgagtct gtgccttact tagatgcaga acgcttaaaa cgtaaaggta
     7501 ccgttgagtc agtacaatca ttcggcttac tgttcttaat gtttaactgt gaaaaagcac
     7561 cgtttaacaa cccgaaagtt cgccaagcgt tacattatgg cttagatacc caaaaattaa
     7621 ttgatgtggt gttcttaggc aatgcaaaag ctgctacatc ttacgtgcaa gatactcacc
     7681 cggattatgt gaaagcaaat tcgcaatatg atttcgataa agcgaaagcc gaagcgttat
     7741 tagcagaagc gggtgtgaca gagttaaaat ttgaattgtt ggcaaccgat cactcttggg
     7801 tgaaagaatg tgcgccatta attcttgaat cgtggaacag cttaaaaggc gtgaaagttt
     7861 cactcaaaca cttacaatca ggtgcattat atggcacaca cgttgataaa ggtgcatacg
     7921 aagttgtgat tgcacctggc gacccatcag ttttcggtaa tgacttagac ctattattaa
     7981 gctggtggta tcgtggtgat gtatggccga aacgtcgttt ccgctggtct gaaacagctg
     8041 aatatgcaga agtacaaaaa ctgcttgatg aagcggccaa aaatcctgct gcttctaaag
     8101 aagcctgggc gaaagcaatc aatattatcg ctgaacaagt gcctctttac ccaattattc
     8161 accgtaaatt gccaactgcg tggaatgata aggcattaac aggtttccaa ccattaccaa
     8221 caacaggtat gtcatttatc ggtgttggtc gtgctaaata gtcgtaacgg ctagctaaat
     8281 gcaagcggta atattttaca aaaaatttac cgcttgcata aataaaaatc aaaatgatgt
     8341 aatattcgtc ttatatcact cagaattctt accatttcta tattttcatt tataatccta
     8401 catatcacta tattagcttg taggtaaact tagatttaat tttggagtgt aactacactc
     8461 aaggagaaga aaatggaaat tatactccga ttattatggc gtcgcctaat ggctctgccg
     8521 gtaatgatct taggcgtaac tgccttggta tttattgttt tacaattcac ccccggagat
     8581 ccggcaaccg ttgctctcgg tgaaagcgcc agcgaagccg ctaaagaaat ctatcgtgaa
     8641 caacatgggt taaatgatcc attgcttgta caatattttc gctttttagg gaatttattc
     8701 gtattagatt tcggtatgac aacccctcca gaacagccta ttgttgatat gattgctaaa
     8761 gcattccctt taacattaca gcttaccttt attggggtct tcttagctgc tatcgtctct
     8821 ttcacattag gtgtaacggc agcactttac cgtgatactt ggattgatca attaatccgt
     8881 ttaatctcgg ttgccgctgt tgccacacct tctttctggt tgggtatttt attgattcaa
     8941 tacttctcct taaaattaga ttggttgcct tcaggcggtt ttattccatt tagcgaagat
     9001 ccaaaaggct atgtaaactc aatggttctc ccttcactcg ctttagctgt tcctgtatgt
     9061 gcctcattga tccgtgtcgt gcgtacaaca atggtagaag aaatggataa agattatgtt
     9121 cgaacagcca tcggtaatgg tgtaccttac tcaacggtga ttcgccacaa cgtgcttcgt
     9181 aatgccttaa ttactccggt aacagtatta ggtttacggg ttggttattt attaggtggt
     9241 gcagttgtga ttgaacagat cttcgactta ccgggaatgg gtaaattaat tttcaacggt
     9301 atcgtcaatc acgacttgaa cttagtgcaa ggtgtagtat taactatcgc atttactttc
     9361 gtgttggtga atatcattgt tgatattctt tacttgctca ttaatccaaa aattcggagt
     9421 ttataatatg tttcgccaag gattagctgc tcgacttgct tcaggcggtg caagatttag
     9481 agcattatca accagctcaa aaatcgcctt actctttatt gtatttatcg cattagtcgc
     9541 tatttttgca ccattagtag cgccttatga cccactgcaa acgctacgtc cagtgcaagc
     9601 tccaagcggt gattatttat tcggtacaga ccgtttagga cgtgatattt tctcgcgtat
     9661 ggtatgggga gcaagaacat ctctctttat cggtttaggt gcggtaggcg ttgcgattct
     9721 attcggtagt attttagggg caactgcggc aaccgccgat aaattcggta acgaagtgat
     9781 tatgcgtttt atggatatct taatggcatt cccgggtatc gcattagctg ctgtattact
     9841 tgccactttc ggtaactctg tacctgtgat tatcgtgact attgcggttg tttacactcc
     9901 ccaacttgcc cgagtggtgc gtgctaacgt agtatctcaa tgggaagaag attatgtgcg
     9961 tgccgagcgt gtaattggtg gtagccgtac ttatattcta ttaaaacacg ttgtgcgtaa
    10021 tactgctgca ccggttttag tattcgcaac agtgatggtt gctgatgcca tcgtatttga
    10081 agcatcactt tcattcttag gtgcaggtgt ccagcctcca ttcccatctt ggggaaatat
    10141 cttatctgaa ggtcgtaacc ttgtattaag tggcttctgg tgggctacaa ctttcgcagg
    10201 gattatgatt ttattaaccg tacttgcgtt aaatatcctt gctgaaggct taaccgatgc
    10261 gttagtaaat ccaaaactaa aacgttcaac tcaaagtaaa gaagatgtcg caaaaccatt
    10321 ggcaacagac gttcaagaag caatgtctga aactcttgcg ttaaaacgtt acttactcaa
    10381 attacacgag aaagaaacga cccgtactga ccgtatgcaa ttaaactcaa cggccaaacc
    10441 gattttacaa gtgaaaaact tatcaatccg tttcccaaat cgttatggtg aaactccgct
    10501 tgttgataat atcagcttta ctgttcacga aggcgaaacc atgggcttag tgggtgaatc
    10561 tggttgtggt aaatcaatca ccgcattctc aattatgggt ttattaccaa aaactgcaaa
    10621 aatcactggt gaaatcctct ttaccgaccg ttcaggtaaa caacacaatc ttttagaatc
    10681 agccgattta aatgcccttc gtggtcacga aatctcgatg atttaccaag atgcattaag
    10741 tgcgttaaac ccatcaatgc gtattaaaga ccaaatggca caattaatca gccgtggtgg
    10801 taaacaatca gcagaaacat tattgcaatg ggtaaaactt gacccggaaa aaacgctcaa
    10861 ccgttatcct cacgagttat ctggaggaca acgtcagcgt gtattaattg caatggcact
    10921 tgctcgtgaa ccgaaactat tgattgctga tgaaccaaca acagcattag acgtaaccgt
    10981 tcaagctgaa gtgatcaaat tattaaacga attacgtgag aaacttggat ttgcaatggt
    11041 attcgtgagc cacgacttag ctttagtggc acaaatcgct caccacatca cggtaatgta
    11101 tgctggtcaa gtggttgaaa ctgcaccaac tacgccatta ttggcaaacc caacacacga
    11161 atacacacgt ggattattag gctcggtact ttctaccgaa ttgcgtgcag aacgcctata
    11221 ccaaattccg ggcagtgtgc cttcaccatt tgattttgcg aaaggtgacc gttttgcgag
    11281 tcgttcatta agaccagatg caaaccctga acagcacttg aaattagtgc caaccggtga
    11341 tcacccacaa cacttctggg cttcacactt agaagatcca caatctaaag aagcataaag
    11401 gagagcaata cgatgagtat gaaatttatc aaagaacagc caattattga actgtctaat
    11461 attgttgtgc aatttgcttc tcgtgacggc accttattta acccgaaaaa attcacggcg
    11521 gtaaatgatg tcagcctagc aattcacgcc ggtgaaacag tcggtctggt tggacaatct
    11581 ggttgtggca aatctacttt agccagtgtg atgatcgggc ttcaaaaacc aacctccggc
    11641 acagtaaaat ttaacggctt agagatgaaa tatggcagtg cggaagctcg caaatatttc
    11701 ggtagccaag tgtcagtaat tttccaagac ccggcaaccg ccctaaatcc gcgtatgcgt
    11761 gtgttggata ttttaaaaga tccgatggat attcataata tcttacaacc gcaagaacgt
    11821 gagaagcggg tttatgattt gctctctcga gtcggtctac ctcgttcagc ggcgttggtc
    11881 gagccaaccc gattatctgg tgggcaaaag caacgtgtag cgattgctcg tgcattggca
    11941 ttaaatccga agctgattgt ggctgatgaa ccaacctctg cacttgacgt atcggttcga
    12001 gctcaagtac tgaacctgct ggcagacttg aaaaaagaac tcaatttagc gatggtattt
    12061 atctctcacg acttacaaac cgttcaccaa gtttcagacc gcattgtggt tatgaacggc
    12121 ggtaaaattg tggaaatggg cgatgcttca gacgtatttg aacgcccaac gcaagagtac
    12181 acccgaacct taattgaagc agcaccatct ttattataat tgtgctttgt gcctacaagc
    12241 ggtaaaattt ttcgcaaatt ttaccgcttg tgtttttatt tatgatccaa attacccaaa
    12301 ccattaacat tgatttatta attgtcggct ctggcatcgc tgggctttct gctgcagttg
    12361 aggcaaaaga aaaaggcatt aataccttac tgattagcaa atctacgatt ggctcgggag
    12421 cgagctactt tccgcttaag gcaacacttg gcattcaagt aaccggcgat gaagccgata
    12481 aaattcactt taaagaagat attgaaaaca tcggcaaagg caaaatcaac cctaatgtgg
    12541 tacaagccta tatcgaagat tcaccacaag cgattgaact actccaccgt atcggctttc
    12601 agccttggaa gcgtaacgac aaccgtccgg cttgttttgc caaataccct cgcccaattt
    12661 atttaattaa agattggcgg gaatctgccc tacgagcgaa acagattttg caagagcaac
    12721 acattgcttt ctatgaaaat gctacccttt tacacatcgt cacagacaat aaccaagtgc
    12781 aaggtgcggt attttcgctc actacaagcg gtcaaatttc ctatattttt tgcaaaacca
    12841 agcatcttat tttagccgca ggcgggattg ctgggctgta taaagataat ctctacccgg
    12901 ctgatgttat cggttcactt cattatgtgg cacaacaagc cggggcaacc ttggttaatc
    12961 ttgcctttat tcagttcatt cctgcctttg tcgagccaaa atacaaggtg ctatttggcg
    13021 aacatacatt gaaatatgtc acccaaatca ccgattctaa gggtaacaat ctatttccac
    13081 acttaaatgc cgagcaattt gcaaaaatgg tgctggaacg tagccattat gctcctttca
    13141 gccttgattt tgccggcgtg gagttcgatt tagtgatgat gaaacatttg cttgaaaacc
    13201 ctcacgaaaa aggcatttac cttaaataca gccccaaact gtatgaagac acggaagagt
    13261 tttataccgt ctatttgaat tggctcaaaa atgaagtcgg gattgattta gtgcaagata
    13321 agattgcaat caagccgttt gctcatagct gtaacggcgg cattcaaatt aatcaatggg
    13381 gcgaaagtgc agtaaaaggg ctatttgcga ttggtgagat ttctcactgc attgaagggg
    13441 caaaccgtat gggtggtaac tcagtcggcg gtggcttagt gtttgccaaa cgtgcggtat
    13501 cggaaattgt tcgttcgcta gaacagaatg atttgcaaaa aaacagcgaa atctcaccgc
    13561 ttgcaatggc agaaaaagca ttaaatcagc ttgctaattc aactgctaat ctagaactaa
    13621 ctgccagtga agtgctttct actattcgag agaaaatggc gttatacgcc aatgtgtacc
    13681 gcactcggag caacctcaaa ctactacttg aggaattaaa ccgtttggaa caagaatttg
    13741 acccaatcta tcacgctcaa tatcaaggca ttgaaattta caatgcgtta aaaaccgctc
    13801 aattagtggt caatcaaatg ctaaatgagc agagtttag
//
LOCUS       DANLXS010000058        13673 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.058, whole genome shotgun sequence.
ACCESSION   DANLXS010000058 DANLXS010000000
VERSION     DANLXS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13673
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.058"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            28..501
                     /gene="nrdG"
                     /locus_tag="PXD71_001218"
     CDS             28..501
                     /gene="nrdG"
                     /locus_tag="PXD71_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL6291347.1"
                     /translation="MENLRFSSEQIVWQEVPNETSLAFLITGCPLGCKGCHSADSWKA
                     CLGKELSSEYLSERLKRYQGLISCVLFMGGEWEAEKLLPLLQLAKKQGLNTCLYSGLE
                     QHEIAETLLHELTYLKTGRWIAERGGLESLTTNQRFTDLRNNQNLNHLFIRGASI"
     gene            498..2279
                     /gene="nrdD"
                     /locus_tag="PXD71_001219"
     CDS             498..2279
                     /gene="nrdD"
                     /locus_tag="PXD71_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL6291348.1"
                     /translation="MIRLQPTQLDGKLHFINQYIQAKNAADGSKMDANANVTQKNIAT
                     MEQELMKDFFVQINRAKVSEKIAKIFGKETACEYLRQIEAHEIYVHDETSLKPYCVSV
                     TMYPFLRDGLTKLGGESKAPKHLASFCGSFINFVFAVSSQFAGAVATVEFLTYFDYFA
                     RKDYGDNYLQTHRSDIENHLQQVVYSINQPAAARGYQSVFWNISVYDKFYFEAMFSRF
                     VFPDFEKPNWETTSKLQLFFMQWFNKERSKAILTFPVVTAAMLTEKGKCKDDEFANQM
                     AKELSQGNSFFIYQSDNPDSLASCCRLRNEISDHSFSYSLGAGGVATGSINVITINMN
                     RLVQDNRDLSEEVSKIHKYQYAYRKLMEEYLAAGMLPVYDAGFISLHKQFLTIGINGM
                     AEAAESQGLTVGYNPDYIDFVQSRLKIIFQANQAAGEKYGVKFNTEFVPAENLGVKNA
                     KWDKEDGYFVPRDCYNSYFYVVEDEEINALDKFLLHGRELIEWLDGGSALHLNLDEAL
                     TQTGYRSLLDTAAKTGCNYFCINVKITICNECSHIDKRTLHSCSKCGSDNIDYGTRVI
                     GYLKRVSAFSSARQKEHSLRFYHREAA"
     regulatory      2336..2432
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2489..3307
                     /gene="thiM"
                     /locus_tag="PXD71_001220"
     CDS             2489..3307
                     /gene="thiM"
                     /locus_tag="PXD71_001220"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDL6291349.1"
                     /translation="MTTSLPYHSHYLQKIRQNRPLVHNITNIVAANFSANGLLALGAS
                     PIMAAAIEEMESVPALSNAVVINIGTLIGKEVEAMELAGKTANRLGIPLILDPVGVGA
                     TPFRRQTVETLVDQIHFTAIRGNAGELATLAGVEWQAKGVDAGEGSHNVVEIAQQIAK
                     RYQTLVAISGETDVVSSGSHTALLHNGSPMFPKITASGCLLSAVCGAFLAVADKEHYF
                     NALIEACAAYAVAGEVAAQALKPTQYGQFAVNLLDTLGALTPETVEQYVRVEYV"
     gene            3300..4613
                     /locus_tag="PXD71_001221"
     CDS             3300..4613
                     /locus_tag="PXD71_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6291350.1"
                     /translation="MSKTHSNPTKVAVASMVGTAIEYFDNYIYTMAAVLVFNVQFFNS
                     DDPVSNQLMSLSVLALAFFTRPMGSILFGHFGDKYGRKQTLIASLLLMGLSTVAIGLL
                     PNYAAIGIWATVLLCLCRIGQGLGLGGEWGGAALVATEHAPKHKRAFFGIFPQMGAPI
                     GLLLANGAFYLVNLIYGEQTFLDWAWRIPFVASIVMIAIGLYIRIKISESPIFLKAEQ
                     QGKNRHTPLKTLFSQHLKPFMIGVLIATAGYVLFYILVAFTPIFVKSPVAVSQAGHAT
                     GLGLPVNLYTQYLLITSVIFGIAIFFSGIYSDKIGRRLLLLLATGATVVYGLTMPFFL
                     ENGTPTSIFIFLTIGMILIGLSFGPMAVILPELFPTEVRYTGASLAYTAAGILGASVF
                     TIIAVKINENFGLFGVGGYLSVASLLSFWALWQVHETKDLELEEI"
     regulatory      4749..4845
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4913..6442
                     /gene="thiE"
                     /locus_tag="PXD71_001222"
     CDS             4913..6442
                     /gene="thiE"
                     /locus_tag="PXD71_001222"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDL6291351.1"
                     /translation="MSNYSNSPYQPNVIWTVAGSDSCAGAGLQTDLHTFHDFNLVGCS
                     VVTSVTAQHPHGVLCVTPVDDHTFRQQFEALLVQGYPNAIKIGLLCSQAQVEILCEYI
                     QKIRAESSHYCYVVYDPVAVASSGQALSDSILLQIVQQKLYPLVDLITPNGTELALLS
                     DTEIQTFGDVKTASHKLFAQGIKAVLAKGGHFEWLGETVRDYLLTANEIYAFSHARQQ
                     SVNTHGTGCTYASAVGAMLATGFDLADAVTVATAYLQKGLSEKQGRGQTALSSLCHTG
                     YPDDIAFFPQTELISAPLKLPKEPFAPTEYQLGLYPVVDSVKWLERLILVGVKTLQIR
                     IKNRPLAEIEQEIAHSVALAKQHNVRLFVNDYWQLAMKYQAYGVHLGQEDLATADLNA
                     IQQSGLRLGVSTHCYFEIMRALAVKPSYIAFGHVFPTQTKVMPSQPQGLTNLAKYVAL
                     VAPLNIPTVAIGGINEQSIENVMHTGVGSAAVVSAVTQAKDWQQAVKNLAKFANGGRN
                     E"
     gene            6435..7607
                     /gene="cytX"
                     /locus_tag="PXD71_001223"
     CDS             6435..7607
                     /gene="cytX"
                     /locus_tag="PXD71_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HDL6291352.1"
                     /translation="MNRTLSTLNSGLIWAGAGISASAIVVGTWIAPLGWQQGLLAIVL
                     GHLLGGILFFAAGLIGAKTGKNAMQTVQISFGRGSVFFSLANVLQLVGWTAIMIYTGA
                     EISNALSLALWEYSAFSLWAIVLGLLIVLWLFTGSNQVGKFKLVTLIAMFLLTLWLSI
                     KVLNGQENFPVSGEMPFSTAVELATIMPLSWLPLVSDYTAKAKKPFAATLSATIGYLI
                     TSAWMYALGLGMVLFTGQTEIAPMLLMTGMSLIGIMVMILSTVTTTFLDAYSAGVSAG
                     NITAKFKETPTAILVTIIGTLLAISLPVTQYENFLLLIGSVFAPMIAVQIADFFVLKR
                     HQAQPNVDWVAAGLWLVGFVLYHLLSYHQVHLLLGLTIPVMLVIFLSTIFVRRFTK"
     gene            7646..7855
                     /locus_tag="PXD71_001224"
     CDS             7646..7855
                     /locus_tag="PXD71_001224"
                     /inference="COORDINATES: protein motif:HMM:NF015737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6291353.1"
                     /translation="MESLVLNNDWVVTPQAQLAYHTINGKADEERLNLLVARAGVRVA
                     KGFALNNGWNLQPYAEVNGIAEKGQ"
     gene            7863..8039
                     /locus_tag="PXD71_001225"
     CDS             7863..8039
                     /locus_tag="PXD71_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6291354.1"
                     /translation="MQVNQYQFGVAENKGRVQGSLGLSAGNGNHRLGLEASVASGKKL
                     KQPFNVLVNYRYQW"
     gene            complement(8080..9936)
                     /gene="sppA"
                     /locus_tag="PXD71_001226"
     CDS             complement(8080..9936)
                     /gene="sppA"
                     /locus_tag="PXD71_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL6291355.1"
                     /translation="MLTILKAFYRIFRCIRECVINLFFILFILLLVPVVAFISSSGST
                     QKPVFNQGALRLNLDGYLADNREEFTDFYRLVQSELGSSEPFKISTFDVVQAISHAKN
                     DPKITGLVLDLQKLQSADFSSLDFIGNEINNFKQSGKPVIAVGEDYSQKQYYLASFAD
                     EIYLNKVGAVDLKGLNYSNTYFKTLLDKIEAEPHIFRVGTYKSAVEPFMRDDMSEEAK
                     QNARGWLNGTWQQTVATLAQNRNIEPQALDLSPANYIEKYKQAKGDDAQLALNQKWVS
                     QLVSSQESRDKLIAQFGKNSEGSYNQIEFLDYMTELNDRFAEVNAPKIAVVNVEGAIA
                     GGESDEMNVGSETLVKLLQQARDDKNVEGLILRINSPGGSAVASELIRQEVEAFQQAG
                     KPVVASMGGMAASGGYWIAATSDKIIASPNTLTGSIGIFGLAVTFEKTAKNLGVSEDG
                     IATSALAQQAGLKSLPQEQAEVLQIGIENGYDRFLELVSRGRKMPKEVVDKVAQGQVW
                     LGTDALKHGLVDELGNFNVAYNALSELINQKREAENQPKVEQFGLQWFAEQDDSLFGT
                     LARDFKAQLQFSVANWLDLPLLKQPQQQLETLSRFNDPKQMYLYCLNCGKID"
     gene            10039..10602
                     /locus_tag="PXD71_001227"
     CDS             10039..10602
                     /locus_tag="PXD71_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL6291356.1"
                     /translation="MQILDLLHHRRSNKKFGDKAPNAEQLEQILKAGLRVPDHGRLKP
                     YRFVVIEKSGMPQFGECLKSAVAEFDMGEERLKKAEKLANQAPMIIGVVATLDDSIAK
                     VPSWEQMITAGCATYAIQLAANAQGFDTVWITNKWINGSALRSAFGCQESDKIIALIM
                     LGSPAEGEQIALAPESESLEGFVRYLK"
     gene            complement(10673..12997)
                     /locus_tag="PXD71_001228"
     CDS             complement(10673..12997)
                     /locus_tag="PXD71_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL6291357.1"
                     /translation="MTDLNLKISQIIAGELNVGSHQILAAITLLGEGNTIPFIARYRK
                     EVTGGLDDTQLRHFETRLIYLREMDERRQTILKSIEEQGKLTDELKAKILSSESKTEL
                     EDLYLPYKPKRRTRGQIAIEAGLEPLADSLWNDPKQDPETLAANFVDAEKGVADTKAA
                     LDGARYILMERFSEDAELLAKLRQYLTAYATLESKVIDGKEEEGEKFRDYFAHSEPFN
                     SVPSHRALAMFRGRNEGVLSLSLNADPTAEEGTKSSHCEEIIREHLGVIFNNQPADKW
                     REQVIAWTWKIKAALHLETELMATLCEKAEEEAIDVFARNLSALLMAAPAGARNTMGL
                     DPGLRTGVKVAVVDNTGKLLDTATIYPHTGREAEAQLAIFSLIKQHNVELIAIGNGTA
                     SRETERFAKAVIKEIKENKPQTVVVSEAGASVYSASEFAANEFPNLDVSLRGAVSIAR
                     RLQDPLAELVKIEPKAIGVGQYQHDVNQSQLARKLDAVVEDCVNAVGVDLNTASAPLL
                     ARVAGMTKTLAQNIVAFRDENGRFTSRSELKKVPRLGPKAFEQCAGFMRIIGGKNALD
                     ASSVHPEAYPVVEKILQATTSTLAELMGNNSKIHSLNAKDFVDEQFGLPTVNDIFKEL
                     EKPGRDPRGEFKTAVFMDGVEEITDLKAGMILEGTVTNVANFGAFVDIGVHQDGLVHI
                     SMLSNSFVEDPHTVVKAGDVVKVKVLEVDAARKRIALTMRLDDKPTDKKEPTSGRNSA
                     EICKNTQKNDRLQSNNRNAFGNNAFADALKGWKK"
     gene            complement(12997..13551)
                     /gene="pilW"
                     /locus_tag="PXD71_001229"
     CDS             complement(12997..13551)
                     /gene="pilW"
                     /locus_tag="PXD71_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HDL6291358.1"
                     /translation="MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARIN
                     LALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLAYYYQQTGDAENAEKAYQTALKL
                     SQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
                     QKIAETLTELEKLDKPRANLLKEN"
BASE COUNT         3682 a         3122 c         2968 g         3901 t
ORIGIN      
        1 acaatatatt gtgttaatga attagtaatg gaaaatctca gattcagctc ggaacaaatt
       61 gtttggcaag aagtgccgaa tgagacctca cttgcttttc tgattacagg ctgcccgctt
      121 ggctgtaaag gttgccatag tgcggatagc tggaaagcct gtcttgggaa agagcttagc
      181 agtgaatatc tctctgaacg cctaaaacgc taccaaggct taattagctg cgtattgttt
      241 atgggcggag aatgggaagc agaaaaatta ctgccgctgt tgcaactggc aaaaaaacag
      301 gggctaaaca cctgtcttta tagtggtttg gagcagcacg aaatcgccga gactttatta
      361 cacgagctaa cctacttaaa aaccgggcgt tggattgccg aacgaggagg attggaaagt
      421 ttaaccacca accaacgctt tactgattta cgcaataacc aaaatcttaa ccatcttttt
      481 attcgaggag cttcaatatg attcgcttac aaccgacaca attagacgga aaactacatt
      541 tcattaacca atatattcaa gccaaaaatg ccgcagacgg ttcaaaaatg gacgccaacg
      601 ctaacgtcac gcaaaaaaac atagcgacaa tggaacagga gctaatgaaa gatttttttg
      661 tgcaaatcaa ccgagcaaaa gtgagtgaaa aaattgcaaa aatttttgga aaagaaaccg
      721 cttgtgaata tttacgtcaa atcgaagcac acgaaattta cgtacacgat gaaaccagcc
      781 taaaacccta ctgcgtttca gtcactatgt accctttctt gcgtgatggt ttgactaaac
      841 tcggcggcga atccaaagcc ccgaaacatt tagcctcatt ctgcggttct tttatcaatt
      901 ttgtctttgc agtcagttct cagtttgccg gtgcagtcgc cacggttgaa tttctgacct
      961 attttgacta ttttgcccgc aaagattacg gcgataatta cttacagact catcgttcag
     1021 atattgaaaa ccacctgcag caggttgtgt atagcatcaa ccaaccggca gccgctcgtg
     1081 gttatcaaag cgtcttttgg aatatttcag tgtatgacaa attctatttt gaagcgatgt
     1141 ttagccgttt tgtttttcct gattttgaga aaccaaattg ggaaaccact tcaaaattac
     1201 aacttttctt tatgcaatgg ttcaataaag agcgaagcaa agcgattctc actttccctg
     1261 ttgtgaccgc agcaatgctt accgaaaagg gcaaatgtaa agatgacgag tttgccaatc
     1321 aaatggcaaa agagctatct caaggcaatt cattctttat ttatcaatca gataatccgg
     1381 attcccttgc ttcttgctgc cgcttgagaa atgaaatcag cgatcatagc ttctcctatt
     1441 cattaggggc tggaggtgtt gcgacaggtt caatcaatgt aattactatt aatatgaacc
     1501 gtttggtgca agataaccgt gatttaagcg aagaagtcag caagatccat aaatatcaat
     1561 acgcttaccg caaactgatg gaagagtatt tagctgccgg tatgttgccg gtttacgatg
     1621 ccggttttat ttcactgcat aaacaattcc tgactattgg cattaacgga atggcagaag
     1681 ctgctgaatc acaaggttta accgtaggtt acaatccaga ctatattgat tttgtgcaat
     1741 ctcggttaaa aattattttc caagctaacc aagcagccgg tgaaaagtat ggggtaaaat
     1801 ttaataccga atttgtgcct gctgaaaatt taggggtcaa aaacgccaaa tgggacaaag
     1861 aggacggcta tttcgtacca cgagattgct acaattccta tttctatgtg gtagaagatg
     1921 aagaaattaa tgctttggat aaattcttac tgcacggcag agagctgatt gaatggctgg
     1981 acggaggctc cgctctacac ttgaatttag acgaagcctt aacccaaacc ggatatcgct
     2041 ccttgctgga taccgccgct aaaaccggct gcaattattt ttgtattaac gttaaaatca
     2101 cgatttgcaa cgaatgtagc cacatcgaca aacgcacgtt gcacagttgt agcaaatgtg
     2161 gctccgataa tattgattac ggcactcggg taatcggcta tttaaaacga gtctccgcct
     2221 ttagttccgc ccgccaaaaa gagcacagct tgcgtttcta tcaccgagaa gcagcttaat
     2281 tgaaaactgc agttgtagcc ctttacaacc gcttttttag tgattatatt aaagcctcat
     2341 ttagtcgggg tgcttgccta agggcaagct gagaaatacc cgttgaacct gatgcagtta
     2401 gcactgacgt aggaaactaa aggcgtaaac ccatctattc cccgttggtg ttggtgcgtt
     2461 gtttttcaat catattaagg aataaacgat gaccacatca ctcccctatc actcgcatta
     2521 tttgcaaaaa attcgacaaa atcgaccgct tgttcacaac attaccaaca ttgttgccgc
     2581 taatttttct gccaacggct tattggctct tggtgcttct cccattatgg cagctgctat
     2641 tgaggagatg gaatccgtgc cggcactcag taacgccgtg gtgattaata tcggcacgct
     2701 tatcggcaaa gaagtagaag caatggagct tgctggcaaa accgccaacc gtttaggtat
     2761 tccgctcatt cttgaccctg tcggcgttgg agcaacacct ttccgccgtc aaacagttga
     2821 aaccttagtc gaccaaattc acttcaccgc cattcgtgga aatgcggggg aactagctac
     2881 cctcgccggt gtagaatggc aagccaaagg cgttgatgcc ggcgaaggct cgcataatgt
     2941 ggttgaaatc gcccaacaga ttgccaaacg ttaccaaacc ttagtcgcaa tcagtggtga
     3001 aaccgatgtg gtaagctctg gctcacacac agctttattg cacaacggca gcccgatgtt
     3061 ccccaaaatt accgcttcag gctgtttatt aagtgcggta tgcggggcat tcttagccgt
     3121 tgccgacaaa gagcactatt tcaacgcact tattgaggct tgcgccgctt atgccgttgc
     3181 aggcgaagtg gctgctcaag cactcaaacc aactcaatac gggcaatttg ccgtcaatct
     3241 gctggatacc cttggggcat taacgcctga aaccgttgag caatatgtga gggttgaata
     3301 tgtctaaaac tcactcaaac ccaactaaag tggcggttgc ctcaatggtc ggcacggcga
     3361 ttgaatattt tgataactac atctacacga tggcagcagt gctggtgttt aatgtgcagt
     3421 tttttaatag cgatgatccg gtttccaacc aattaatgtc gctttccgta ctagctcttg
     3481 cctttttcac ccgcccgatg gggtcgattt tattcgggca tttcggcgat aaatacggac
     3541 gcaaacaaac tctgattgcc tcgctcttat taatgggctt atctaccgta gcaatcgggc
     3601 ttttgcctaa ttatgccgcc attggcattt gggcaaccgt gttactctgc ctctgtagaa
     3661 tcggacaagg tttaggctta ggcggcgaat ggggcggagc tgctctcgtt gccactgaac
     3721 acgccccgaa acacaagcgg gcattttttg gtatttttcc gcaaatgggc gccccaatcg
     3781 gcttgctgtt ggcaaacgga gcgttctact tggtgaattt aatttacggt gaacaaacct
     3841 tcttggattg ggcgtggcga attccgtttg tcgcttctat tgtgatgatc gccattgggc
     3901 tttatatccg aattaagatc agcgaaagcc cgattttcct taaagcggaa caacaaggta
     3961 aaaaccgcca taccccgctt aaaactttgt ttagccaaca cctcaagcct tttatgatcg
     4021 gcgtgctgat tgccaccgcc ggctatgtgt tgttctacat tttagttgct ttcaccccga
     4081 ttttcgtcaa aagcccggtg gcagtttctc aagcaggaca cgctacaggc ttaggtttac
     4141 cggtaaatct ctacacccaa tatttgctga ttacctccgt gattttcggc attgcgattt
     4201 tcttctccgg catttattca gacaaaatcg gacgccgttt actgctgtta cttgccacag
     4261 gagcaaccgt agtttacggc ttaacaatgc cgtttttcct cgaaaacggc acaccgactt
     4321 cgatctttat tttcctcacc attggaatga tcttaatcgg cttaagcttc ggcccgatgg
     4381 cagtaattct acccgaactc ttcccaacag aagtacgtta cacaggggct tcgcttgcct
     4441 acaccgctgc cggtatttta ggagctagcg tgttcactat tattgcggtc aaaatcaatg
     4501 aaaatttcgg cttattcggt gtaggcggct atttatcggt tgcctcatta ctgagcttct
     4561 gggcattgtg gcaagtccac gaaaccaaag atttggaatt agaggagata taaaaaacca
     4621 ctcacttaac gacggggcaa gttaccacct tgccccaaga tgagggaatg caaatttccc
     4681 tctaatagac ttcacaacgg atattttgca tttttttata aaaaataacc gcttgtaagc
     4741 tatactaaaa acgcttgtcg gagtgccgta aggctgagat cgcaacagcg agatccgtgg
     4801 aacctgtagg ttagtacctg cgtagggaac aagttttacc caaattcaga ttttgggatc
     4861 attaccacat ctctggcaag caaaacccta agcatattat gaggattttg cgatgtctaa
     4921 ttattcaaac tctccatacc aacctaacgt catttggacg gttgccggct cagattcctg
     4981 tgccggtgcc ggtctgcaaa ccgatttaca tacctttcac gattttaacc tagtgggttg
     5041 ttcagtagtt acttctgtaa cggctcaaca tccgcacggt gtgctgtgtg taacgcctgt
     5101 tgatgatcac accttccgcc aacagtttga agccttgctg gttcaaggct acccgaacgc
     5161 aatcaaaatc ggcttactct gctcgcaagc acaagtagag atcttgtgtg aatatatcca
     5221 aaaaatccgt gcagaaagct cgcattattg ttatgtggtt tacgaccctg tggcagtggc
     5281 aagcagtggg caggcgttat ccgacagtat tttattgcag atagtgcaac aaaaattgta
     5341 tccgctggtg gatttaatca ctccaaacgg cactgaactc gctttattaa gcgacacaga
     5401 aatccagacc tttggtgatg tgaaaaccgc tagccacaaa ctgtttgcac agggaatcaa
     5461 agccgtgtta gccaaaggtg gacattttga atggctgggg gaaacggtaa gagattatct
     5521 gctgactgca aatgaaattt atgcctttag tcacgcccga cagcaaagtg tgaataccca
     5581 tggcacaggt tgcacctatg cttcagcggt tggagcgatg ttggcaaccg gctttgattt
     5641 agctgatgcc gtcactgttg ccaccgctta tttgcaaaaa ggcttatcgg aaaaacaagg
     5701 cagagggcaa actgctttaa gttcactttg ccacaccggc taccctgatg atatcgcttt
     5761 cttcccacaa accgagctga tttctgcccc tcttaaattg ccgaaagagc cttttgctcc
     5821 aacggaatat caacttgggc tttaccctgt tgtagatagc gttaaatggc ttgaacgctt
     5881 aattcttgta ggggtaaaaa ccttgcaaat tagaatcaaa aatcgaccgc ttgcagagat
     5941 tgagcaagaa attgcccatt cggtcgcact ggcgaaacag cacaatgtgc gtttgtttgt
     6001 aaatgattac tggcagttag ccatgaagta tcaggcttac ggcgtgcatt tagggcagga
     6061 ggatttagcc accgccgatt taaatgcaat tcagcaatct gggttacggc ttggtgtttc
     6121 aacccactgc tactttgaaa taatgcgggc attggcggtg aaaccgtctt atatcgcttt
     6181 tggccacgtt ttccctactc aaaccaaagt aatgccgtct caaccacaag gcttaacaaa
     6241 tttagccaaa tatgttgctc tcgttgcacc gcttaacatt ccaactgtgg cgattggtgg
     6301 aattaatgag caaagcattg aaaatgtaat gcataccggt gtcggcagtg ccgcggtggt
     6361 aagtgccgtt acccaagcaa aagattggca acaagcggtc aaaaacttag caaaatttgc
     6421 aaatggaggc cgtaatgaat agaactctta gtacgctaaa cagtggtttg atttgggcgg
     6481 gagcgggcat tagtgccagt gcaatcgtgg ttggcacttg gattgcccca cttggctggc
     6541 aacaaggctt gctggcgata gtgctagggc atttattagg cggcattttg ttttttgctg
     6601 ccggcttaat tggggcgaaa accggcaaaa atgcgatgca aacggtgcaa atttcctttg
     6661 gcagaggctc ggtgtttttc tcccttgcca atgtgctaca gcttgttggt tggacagcca
     6721 ttatgatcta tacaggggca gagatcagta atgcccttag ccttgcctta tgggaatatt
     6781 cggctttctc gctttgggca atcgtgcttg ggttgctgat tgtgctatgg ttatttaccg
     6841 gctctaatca ggtcggaaaa ttcaagttag tgacattgat agcgatgttt ttgctcacct
     6901 tatggctaag cattaaggta ttgaatgggc aggaaaattt tcctgtttcg ggtgaaatgc
     6961 cgtttagcac cgcagtggaa ttagcaacta ttatgcctct ttcgtggcta cctttggtat
     7021 cagactatac cgctaaagcg aaaaagcctt ttgccgcgac cctttcggcg accataggct
     7081 atttgatcac cagtgcgtgg atgtatgcgt taggtctggg tatggtgcta tttaccgggc
     7141 aaaccgaaat tgcccctatg ttactaatga caggaatgag ccttatcggc attatggtga
     7201 tgattctttc gactgtgact accacgtttc ttgatgccta ttccgccggt gtcagtgccg
     7261 gcaatatcac ggcaaaattc aaagaaaccc caaccgccat tctagtcaca atcatcggca
     7321 cgctactggc aatcagttta ccggttaccc aatatgaaaa tttcctgctg ctgataggct
     7381 cggtattcgc cccgatgatt gccgtgcaaa ttgcggattt ctttgtgtta aaacgccacc
     7441 aagcacagcc taatgttgat tgggttgctg ccggattatg gcttgtcggc ttcgtgctat
     7501 accaccttct ctcttaccac caagtgcatt tactactcgg cttaaccatt ccagtgatgt
     7561 tagtgatttt tctcagcacc atttttgtga ggaggttcac aaaatagaaa gataagcggt
     7621 tcaattggag tgaaaatttg ctaaaattga gtcgcttgtt ttaaacaatg actgggtagt
     7681 aacaccacaa gctcaacttg cctaccacac cattaacggt aaagccgatg aggaacggtt
     7741 aaatctttta gttgcccgtg ccggcgtaag agtagcaaaa ggctttgcct tgaataatgg
     7801 ttggaatctg caaccttatg cggaagtaaa cggcattgct gaaaaaggcc aatagtacga
     7861 aagtgcaagt aaaccaatat cagtttggag ttgccgaaaa caagggcaga gtgcaaggtt
     7921 cacttggctt gagtgcagga aacggtaatc accgtttagg tttagaggca tcagttgctt
     7981 caggtaagaa actgaaacaa ccatttaatg tattagtaaa ttatcgttat caatggtaat
     8041 ttaagagggc aggtgaaaac ctgcccttaa attcaataat taatctatct tcccacaatt
     8101 taaacagtaa agatacatct gtttcggatc gttaaaacgt gaaagagtct ctagctgttg
     8161 ttgcggttgc ttcaacagcg gtaaatcaag ccaatttgcc acgctaaatt gtaattgagc
     8221 tttgaaatca cgggcaagcg tgccaaataa gctgtcatct tgctccgcaa accattgtaa
     8281 gccgaactgt tcgactttag gttggttttc cgcttcacgt ttttggttga tcaattcaga
     8341 taacgcatta tatgccacat taaaattgcc caactcatca accaatccgt gttttaacgc
     8401 atcagttccc agccacactt gcccttgagc cactttatct accacctctt ttggcatttt
     8461 cctgccacga gaaaccagct ctaaaaaacg gtcgtagccg ttttcaatcc caatttgcag
     8521 cacttctgcc tgctcttgcg gtaggctttt taaaccggct tgttgtgcta gggcagaagt
     8581 cgctatgccg tcttcgctca caccgagatt tttagcggtt ttttcaaaag ttactgctaa
     8641 accgaaaata ccgatagagc cggtaagcgt attcgggcta gcaataattt tatcgctggt
     8701 agccgcaatc caataaccgc ccgatgctgc cattccgccc attgaagcaa ccaccggttt
     8761 gccggcttgt tgaaaggctt ccacttcttg gcgaatcagt tcagacgcca cagcactgcc
     8821 gccggggctg ttaatgcgta gaattaagcc ttccacattt ttgtcatctc gagcttgttg
     8881 cagtaatttt accagcgtct cgctgcccac attcatttcg tcactttcac cgccagcaat
     8941 cgccccttcc acattcacta ccgcaatttt cggagcattc acttctgcaa agcggtcgtt
     9001 tagttcagtc atataatcta aaaattcaat ctggttataa ctgccttcgc tgtttttgcc
     9061 aaactgtgca atcagtttat cccgactttc ttggctggac actaactgac taacccattt
     9121 ttgatttaat gccagttgag catcatctcc tttagcttgt ttatattttt caatgtaatt
     9181 ggcaggcgat aaatccagtg cttgcggttc aatgttgcgg ttttgagcta gtgttgccac
     9241 agtttgttgc caagtgccgt ttaaccagcc tcgggcattt tgtttcgcct cctcagacat
     9301 atcatcacgc ataaacggct caaccgccga tttataagta ccgacacgga aaatatgcgg
     9361 ttcagcctca attttatcga gcaaggtttt gaaataagtg ttggaataat ttaagccttt
     9421 taaatccacc gcaccgactt tattgagata aatttcatca gcaaaggaag caagataata
     9481 ttgtttttgc gagtagtctt cgcccactgc aatcaccggc ttgcccgatt gtttgaagtt
     9541 gttgatttca ttgccgataa aatccaatga gctgaaatca gcactttgta gcttttgcag
     9601 atctaacacc aagccggtaa ttttcgggtc atttttagcg tggctaatcg cttgcaccac
     9661 atcaaaagtc gagattttaa acggctcgga actacctaat tccgattgca ccaagcggta
     9721 gaaatcagtg aattcttccc gattatctgc taaataacca tctaaattta aacgtaacgc
     9781 cccttgattg aacaccggct tttgcgtgct gccagaagag ctgataaatg cgaccaccgg
     9841 cactaatagc aaaataaaca gaataaaaaa gagattaatg acacactctc taatacaacg
     9901 gaagattcgg taaaatgctt ttaaaatcgt taacatcgga aattcctaaa aataaactca
     9961 gtcggcacag tataaagcaa tcgctaaatg aagaaaatat gctattcttg aacgcaaaaa
    10021 attaacaaag gaacgcttat gcaaatcttg gatttattac accatcgccg ttcaaataaa
    10081 aaatttggcg ataaagcccc gaatgcagaa caactggaac aaattctaaa agccggcttg
    10141 cgagtgccgg atcacgggcg gttaaagcct tatcgctttg tggtgattga aaaaagcggt
    10201 atgccacagt ttggtgaatg cttaaagtct gcggtagcag aatttgatat gggcgaggaa
    10261 cgcttaaaaa aagcggaaaa attagctaat caagctccaa tgattatcgg cgtggtagca
    10321 acattagacg acagcatcgc caaagtgcca agttgggaac aaatgattac cgcaggctgt
    10381 gcaacttatg cgattcagtt agcggcaaat gcacagggct ttgatacggt ttggatcacc
    10441 aataaatgga ttaacggctc tgctcttcgc tccgcttttg gttgccaaga atctgataaa
    10501 atcattgcct taattatgct tggctcaccg gcagagggcg agcaaattgc attagcccct
    10561 gaaagtgaaa gtttagaggg gtttgtacgt tatcttaagt aagcaggtta acagataaaa
    10621 gttagtaata agggcgaaaa atatttcgcc cttgctttta tctgttttcg ccttacttct
    10681 tccagccttt taacgcatcg gcaaaggcat tgttaccaaa agcgttgcga ttgttgcttt
    10741 gcaagcggtc atttttctgc gtatttttgc aaatttctgc tgaatttcga ccgcttgttg
    10801 gctctttttt atcggttggt ttgtcgtcta aacgcatcgt aagggcaata cgtttgcggg
    10861 cagcgtccac ctcaagcact ttgactttca ccacatcacc agctttcact accgtgtgcg
    10921 gatcttcaac aaaagagttt gagagcattg aaatatgcac caagccatct tggtgtacgc
    10981 caatatcgac aaaagcccca aagtttgcca cattggtcac tgtgccctcc aaaatcatac
    11041 cggctttgag gtcggtaatt tcttccacgc cgtccataaa cacggcggtt ttaaactctc
    11101 cacgagggtc acgccccggt ttttctaact ctttgaaaat atcgttcacc gttggtaaac
    11161 caaactgctc atccacaaaa tctttggcgt tgagcgagtg aattttgctg ttgttaccca
    11221 ttaattctgc aagtgttgag gtagttgcct gtaagatttt ttctaccacc gggtaagctt
    11281 ccgggtgaac gcttgaggca tcaagggcat ttttcccacc gataattcgc ataaaaccgg
    11341 cacactgttc aaaggctttc ggccctaaac gaggcacttt tttcagctct gagcggctgg
    11401 taaaacggcc gttttcgtca cggaatgcca cgatattttg tgccaatgtt ttggtcattc
    11461 ccgccacccg agccagcaac ggagcggagg ctgtattcag atccacgccc actgcgttta
    11521 cacaatcttc tactaccgca tcgagtttac gggcaagttg ggattggttt acatcgtgtt
    11581 gatactgccc aacaccaatc gcttttggtt cgattttcac caactcggca agtggatctt
    11641 gcaaacggcg ggcaatcgaa accgctcccc gtaatgaaac gtctaaattt ggaaactcgt
    11701 tagccgcaaa ttcagaggca gaatagaccg atgccccagc ctcactcacc accaccgttt
    11761 gcggtttatt ctctttgatt tctttaatca ccgctttggc aaaacgttcg gtttcacgag
    11821 aggccgtacc gttaccaatc gcaatcagtt ccacattgtg ttgtttgatt aagctgaaaa
    11881 tcgcaagctg tgcctccgcc tctcgcccag tgtgagggta aattgtggcg gtatccagca
    11941 atttaccggt gttatccacc accgccactt tcacgccggt acgcaagccc gggtctagcc
    12001 ccattgtatt tctcgcaccg gcaggggctg ccattaataa tgccgagaga ttgcgggcaa
    12061 acacatcaat cgcctcttct tcagcttttt cacacagggt tgccattaat tcggtttcta
    12121 aatgcagagc tgctttgatt ttccacgtcc aagcaatcac ttgctcacgc catttatctg
    12181 ctggttgatt attaaaaatc acgcccaaat gctcacgaat aatttcttca caatggctgg
    12241 attttgtgcc ttcttctgca gtcggatcgg catttaatga aagtgataac accccttcat
    12301 ttcgcccacg gaacatcgcc aaagcacggt gggaaggcac gctgttaaac ggctcgctat
    12361 gagcaaaata atcacggaat ttctcgccct cttcctcttt gccgtcaatc actttcgatt
    12421 ccaacgtggc gtaagcggtc aaatattggc gaagttttgc aagtagctca gcatcttcgc
    12481 taaaacgctc cattaaaata tatcttgccc cgtctaatgc ggccttggta tccgcaacgc
    12541 ctttttccgc atcaacaaag tttgccgcta aggtttcagg atcttgtttc ggatcattcc
    12601 ataaactgtc cgccaatggc tctaaccctg cttcaatcgc aatttgccct ctggtgcgac
    12661 gttttggttt atacggcaaa tagagatctt ccagctcggt cttgctttca ctgcttagaa
    12721 ttttggcttt taattcatcg gttaatttgc cttgttcttc aatcgacttc aaaatggttt
    12781 ggcggcgttc gtccatttca cgcaaataaa ttaagcgggt ttcaaaatgg cgaagttggg
    12841 tgtcgtctag cccgccggtc acttctttac ggtaacgagc aataaatgga atggtattgc
    12901 cctcacctaa tagcgtgatg gctgcaagaa tttggtgtga gccaacgttg agttcgccgg
    12961 caataatttg gctgattttt agatttaagt cagtcattag ttttccttca ataaatttgc
    13021 tcgaggttta tccaatttct caagctcggt caatgtttcg gcaatttttt gcagattcgg
    13081 ctcttttttc gcacataaca ccatattttc taagctatcc gcttggtgat agtagggtgc
    13141 tttgcttttg acagcctgct caaattgctg ataagcctgt tgaaattgac cctgtttaca
    13201 taaaaaagtg ccgtaattat tcagcacatc agggcggttt tgactcaatt tcagtgccgt
    13261 ttggtaggct ttttcggcat tttcagcatc acctgtttgt tggtaataat aggcaagcac
    13321 cgaataaggg aggtaatctt tttcatcgtg agccaaggct ttatcaatat tctgcttagc
    13381 ttttgggaaa tcattttgct ccagataagc taaagccaaa ttaatacggg cttttaccgc
    13441 ttccgagcgg ttaaattcga cttcagagga agaaggggga agagaagaac aagccgctaa
    13501 taaaaaaatc agaatacaag cggtcatttt tggaaaaaat tttgcaaaca taacgttctc
    13561 ctacaacaaa gggcttaatt ttactgttcc atcctcgcta agactagcga ttttttgcga
    13621 agaagaaaaa tttttcgttt tcatttttcg acgcagatcg caaaaataat aat
//
LOCUS       DANLXS010000059        13312 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.059, whole genome shotgun sequence.
ACCESSION   DANLXS010000059 DANLXS010000000
VERSION     DANLXS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13312
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.059"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..757
                     /locus_tag="PXD71_001230"
     CDS             <1..757
                     /locus_tag="PXD71_001230"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL6291359.1"
                     /translation="VWAARYIVDRINAFQPTKEKPFVLGLPTGGTPEKTYKELIKLYQ
                     AGEVSFKNVVTFNMDEYVGLPKEHPESYHSFMFKHFFDHVDIPLENINILNGMAEDVN
                     AECERYEEKIRFYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIKTLTEDTRIANSRF
                     FNNDVSQVPKFALTVGVGTLLDAEEVMILVTGHNKALALQACVEGAVNHLWTISCLQL
                     HRRGIVVCDEPATQELKVKTVKYFKELEKNVAR"
     gene            838..1980
                     /gene="nagA"
                     /locus_tag="PXD71_001231"
     CDS             838..1980
                     /gene="nagA"
                     /locus_tag="PXD71_001231"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL6291360.1"
                     /translation="MNHYALTNCVIYTGEQVLYQHALVVKEDKIDSIVLEENLPLDLQ
                     KIDLKGANLSAGFIDLQLNGCGGVMFNEEITTRTLEIMQETNLRSGTTSYLPTFITSP
                     DEGVKQAVAVMREYLSKHKNQALGLHLEGPYLSVAKKGVHRPEFIREISPEMLDFLCD
                     NGDVITKITIAAENPTADHIKRFNDAGIIVSIGHSNATFAQTKQRIAEGAAFATHLHN
                     AMSPVSNGREGGVVGAVLDSDIYTGIICDGLHVEWGNIRIAKKAKGDKLVIVSDAVAA
                     AGSDIESFIFVGKKVTVKDGKCYDEFGALGGAAITMIESIRNVVQHCDISLNEAIRMS
                     TLYPAKAIGVEETLGSLEAGKIANLAIFDNQFEVKATAVNGQLQWH"
     gene            2062..2376
                     /locus_tag="PXD71_001232"
     CDS             2062..2376
                     /locus_tag="PXD71_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HDL6291361.1"
                     /translation="MQFKDYLATFPTIDHLAGLNVINSGEVIQHIPAIEGKLGSLKLY
                     NALFAKFDGKLTASAAKQGLEWFAEHVEDAKNNVGKHPNIDLLFRVIEQDLSLSLEPI
                     VK"
     gene            complement(2455..3645)
                     /locus_tag="PXD71_001233"
     CDS             complement(2455..3645)
                     /locus_tag="PXD71_001233"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL6291362.1"
                     /translation="MTQAIESVLAELKRGVENIYSEEDLITKLKENRPLRIKLGADPT
                     APDIHLGHTVVLNKLRQFQQFGHEVYFLIGDFTGMVGDPSGKNSTRPPLSREDVLRNA
                     ETYKEQIFKILDPQKTKIVFNSEWLGKLGTEGMIRLASNYTVARMLEREDFKNRFTNQ
                     QPIAIHEFIYPLLQGHDSVELKADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPL
                     LVGLDGEKKMSKSLGNYIGVTEAPSEMFGKIMSISDDLMWDWYNLLSFRPLDEIAQLK
                     ADVENGKNPRDVKILLAKEIIARFHDEAAADAAEQEFINRFQKGAIPDEVPEFTFEGE
                     IGLAALLKEAGLVPSTSEAIRSAQQGGVKIDGEKVEDVKQTAQKGTFVYQVGKRKFAR
                     ITVK"
     gene            complement(4008..5312)
                     /locus_tag="PXD71_001234"
     CDS             complement(4008..5312)
                     /locus_tag="PXD71_001234"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HDL6291363.1"
                     /translation="MESIIALFKGMGLMHLELGQAIMIVISLVLLWLAIARKFEPLLL
                     LPIGFGGLMSNIPEAGMAMTALDNLLHSGSAEQLAIIAAKVNSTSLDLHSIKDAIALA
                     APSVQNELEVMAGDMGYTAGVLALFYKVAIGYGVAPLIIFMGVGAMTDFGPLLANPRM
                     LMLGAAAQFGIFATVLGALGLNWLGIIDFTLPQAAAIGIIGGADGPTAIYLASKLAPE
                     LLGAIAVAAYSYMALVPLIQPPIMKALTTEQERKIRMVQLRNVSNREKIIFPIVLLLL
                     VALLLPDAAPLLGMFCFGNLLRTSGVVERLNDTAQNALINIVTIFLGLSVGAKLVADK
                     FLQPQTLGILVLGVIAFGIGTASGVIMAKIMNRFSQHKINPLIGSAGVSAVPMAARVS
                     NKVGQEYDKQNFLLMHAMGPNVAGVIGSAIAAGVMLKYISTM"
     gene            complement(5322..7148)
                     /gene="oadA"
                     /locus_tag="PXD71_001235"
     CDS             complement(5322..7148)
                     /gene="oadA"
                     /locus_tag="PXD71_001235"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HDL6291364.1"
                     /translation="MTTQPKKIAITDVVLRDAHQSLFATRLRLDDMLPIASELDNIGY
                     WSLETWGGATFDSCIRFLGEDPWVRLRELKKAMPKTPMQMLLRGQNLLGYRHYADDVV
                     DKFVERSVANGMSVFRVFDALNDPRNMQAALQAVKKHGGHAQGTLSYTTSPVHTLQTW
                     LDVTEQLLEIGVDSLVIKDMSGILTPMAAYELVSEIKKRYDVELHLHCHSTTGMAEMA
                     LLKAIEAGVDGVDTSISSMSGTYGHPATESIVATLQGTPYETGLSIPALEKISAYFRN
                     VRKKYAKFEGQLRGIDSRILVAQVPGGMLTNLESQLKQQNASDKLDLVLQEIPRVRED
                     LGNIPLVTPTSQIVGTQAVMNVLMGERYKTIAKETAGILKGEYGRTPAPVNKALQDRV
                     LEGAEPITDRPADHLPPELEKLVAEVTASAKEKGITLSENMIDDVLIVALFQQVGWKY
                     LENRNNPSAFEPVPSADEAKPAQASAASAPKAQTQSGSAIYTVELEGKAFVVKVSEGG
                     DITNIAPTPATPAPQAVDSAPKSANTLSANAEPVNAPMAGNILKVEVKEGQQVAEGDV
                     LLILEAMKMETQICAAKSGTIQGINVKQGDVVAVNDTLMSIA"
     gene            complement(7188..7442)
                     /locus_tag="PXD71_001236"
     CDS             complement(7188..7442)
                     /locus_tag="PXD71_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit gamma"
                     /protein_id="HDL6291365.1"
                     /translation="MAPAELFAEGINLMVAGMGFVMLFLLVLIYAISFISKIINQYFP
                     EPVSIPASKTVPQSPPDDLERLRPVIAAAIAHHRRINGQH"
     gene            complement(7632..8819)
                     /locus_tag="PXD71_001237"
     CDS             complement(7632..8819)
                     /locus_tag="PXD71_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL6291366.1"
                     /translation="MSNNNRSALLGAAFLMATSAIGPGFLTQTATFTQTLLASFGFVI
                     LLSILLDIGAQLNIWRIVAVSEKKAQDIANAVLPGAGYFLALLIVMGGLAFNIGNVGG
                     AGLGLNILTGLSPEMGAVVSGAIAVGVFLFHEAGKVMDRFAQIMGFVMIALTIFVAVK
                     ANLPIDDAVVHTFVPEKLDVIAIVTLVGGTVGGYITFAGAHRLLDAGIKGKENLKEVS
                     KSSVSAILIASIMRVVLFLAVLGVVSMGVQLDPKNPAATPFAHVAGDVGLMIFGVVIW
                     AASITSVIGAAYTSVSFITSFSPFIEKHKNCFIVAFIVVSTAVLATIGRPAQVLVFVG
                     TLNGLILPISLGLILLAAHKTKIVGDYKHPVWMTVSGAIVVILMAVLSAMTLSKYISG
                     LMA"
     gene            complement(9094..9831)
                     /gene="pxpA"
                     /locus_tag="PXD71_001238"
     CDS             complement(9094..9831)
                     /gene="pxpA"
                     /locus_tag="PXD71_001238"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDL6291367.1"
                     /translation="MNHVDLNVDLAEGCGNDHSLLQLVTSANVCCGLHAGDYNEMRKA
                     INWAKENSVRVGAHPSFPDRENFGRTNMQLPDDELKACLLYQLGAAKALCDAENMPMS
                     YVKPHGALYNMAAKDPHLATLIANTVKQFDPNLKLMGLSGSLMLSIAEQHGLETISEV
                     FADRHYLADGSLVPRTRDDAMVESDDEAISQVLQMVLDGTVPTVDGGNVAIKADSICL
                     HGDGEHAVVFAERIKKELQAKGILVKA"
     gene            complement(9818..10747)
                     /locus_tag="PXD71_001239"
     CDS             complement(9818..10747)
                     /locus_tag="PXD71_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDL6291368.1"
                     /translation="MIYIEIIQGFAHIQDLGRYGYRHLGVSHGGAMDSLALQAGNLLL
                     KNANNAPAIELTLGGMRITFDCDTPFCLTGALVEAELDGTPVFSYYRYTANARQTLTI
                     KRIVLGNYAYLCVAGGFLVPQVLGSASTALKAQFGGFQGRMLKAGDNIATGRRDSRLS
                     LMSIEPIDFTSRIRATASSEYEAFTADSRERFWQQGWQLQNNSNRMGYRFAEKALELT
                     ASLEMLSYAAPSGTVQVPPDGQPIVLMADAQTTGGYPKIACIIQADLGRLAQNPFGST
                     VQFEQVSREQAVEIYQKDQNYLETIRRKANESR"
     gene            complement(10744..11391)
                     /gene="pxpB"
                     /locus_tag="PXD71_001240"
     CDS             complement(10744..11391)
                     /gene="pxpB"
                     /locus_tag="PXD71_001240"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDL6291369.1"
                     /translation="MLTIHHTSEHSLLCLAEPPAELAKQQQIWRVAEQARALDGVLEV
                     VVGMNNFTLFHAIGTDSTRLIQAVQQLWENDQAQAAQSPRLIEIPVHYGGEFGEDLFE
                     VAQFHNTTPEEIIRRHTEPTYTVFMIGFQPGFPYLNGLPQNLHTPRRNVPRTKVPAGS
                     VGIGGRQTGIYPFTSPGGWQLLGRTELPLFDIQHNPPTLLTAGDNVRFVAESITL"
     gene            complement(11485..12309)
                     /locus_tag="PXD71_001241"
     CDS             complement(11485..12309)
                     /locus_tag="PXD71_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDL6291370.1"
                     /translation="MKKLLLFLLFIGNPIFAKSPELMLVEQYQDQDISGWVMSEKLDG
                     VRGFWDGQQLISRQGYPLNPPDYFIQDFPPFAIDGELFSERGKFEEISSIVRSAQPKG
                     WHKLKLQVFDVPNAQGNLIERLATLENYLKQHPTLHIAIIEQIPIKDKAHLQQFYQSV
                     LEQGGEGVIVRNPNAGYIQGRSAQILKIKPVLDEECTVVAHHKGKGKYTDKLGAITCE
                     NQRGRFRIGSGFKDKERENPPPIGSIITYKYRGVTESGKPRFATFWRIRNDQISEN"
     gene            complement(12443..13267)
                     /locus_tag="PXD71_001242"
     CDS             complement(12443..13267)
                     /locus_tag="PXD71_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291371.1"
                     /translation="MEHFIGLGVAGNFAGHLEQAGEEADFAKVKTVEAVQPKAIFPFY
                     VPAENLGDYQFLSTYPLSNTAINFPSDADNLQIEPEVALICEIGYQDQQVVSLTPTHF
                     AAYNDCSIRRPNANKICEKKNWGASSKGLSAIHLPLDNFNKGGTLDNYNIACFHKRNG
                     ELNTYGIDSPAVEYSYFHQKLLDWIVERMNNQPDEGPMNNISEMLKQANYPTKAIISI
                     GATRYTPFGESNLLQVGDVSMVVVYNVKKYSHSQIEEMAKLETFSEDISALIQTVR"
BASE COUNT         3617 a         2976 c         3000 g         3719 t
ORIGIN      
        1 tgtttgggct gctcgttata ttgttgatcg tatcaatgca ttccaaccaa ccaaagaaaa
       61 accttttgtg cttggcttac caaccggcgg tacgcctgaa aaaacctata aagaattaat
      121 taaattatat caagctggtg aagtgagctt taaaaatgtg gtgactttca atatggacga
      181 atacgtcggc ttacctaagg aacaccctga aagctaccac agttttatgt tcaagcactt
      241 ttttgaccac gttgatattc cgcttgagaa tatcaatatc ctcaacggta tggcggaaga
      301 cgtgaatgca gaatgcgaac gttatgaaga aaaaatccgt ttttatggta aaatccatct
      361 gtttatgggt ggggtcggtg tggatgggca tattgcgttt aatgagcctg catcttcgct
      421 ttcttcccgc actcgaatta aaacgcttac cgaagatact cgcattgcaa actcacgttt
      481 ctttaacaat gacgtaagcc aagtgccgaa atttgcttta accgttgggg ttggtacgtt
      541 gcttgatgcc gaagaagtga tgatcttagt gaccggccat aacaaagcct tagcattaca
      601 ggcgtgtgtg gaaggggcgg taaatcatct ttggaccatc agttgcttac aattacatcg
      661 ccgtggtata gtggtgtgtg atgagccggc aacccaagag ctgaaagtta aaacagttaa
      721 gtatttcaaa gagttagaga aaaacgtagc tcgctaattc tgattttaca agcggttaat
      781 tttgtaatat tttttacaaa aacgaccgcc tgttagcatt taaagagaag agagaaaatg
      841 aaccattatg ccttaacaaa ctgtgtgatt tacaccggtg aacaagtgct ttaccaacac
      901 gcattagtgg tgaaagaaga taaaatcgac tccattgttt tggaagaaaa tttaccgctg
      961 gatctgcaaa aaatcgacct aaaaggagcg aatctttcag ccggttttat tgacttgcaa
     1021 ctgaacggct gcggtggggt gatgtttaat gaagaaatca ctacccgcac tttagaaatt
     1081 atgcaggaaa ccaatctgcg ttcaggcaca accagctatt taccgacttt tattacctcg
     1141 cctgatgagg gagtgaaaca agcggtggcg gtaatgcgtg agtatttgtc gaaacacaaa
     1201 aaccaagcac tcggcttgca tttggaagga ccttatctaa gtgtggcaaa gaaaggagta
     1261 caccgtccgg aatttatccg tgaaatcagc cctgaaatgt tagatttctt atgcgataac
     1321 ggcgatgtaa ttacgaaaat cacgattgca gcagaaaacc cgaccgcaga ccatatcaaa
     1381 cgctttaatg atgcaggaat tattgtatct atcgggcatt ccaatgccac ttttgctcaa
     1441 accaaacagc gaattgccga aggggcagcg tttgctacgc atttacacaa tgcaatgtcg
     1501 ccggtcagca acgggcgtga aggcggtgta gtaggggcgg tgttggatag cgatatttac
     1561 accggcatta tttgcgatgg cttgcacgtt gaatggggca acattcgcat tgccaaaaaa
     1621 gccaaaggcg ataaattagt gattgtcagc gatgctgttg ctgccgcagg aagtgatatt
     1681 gagtcgttta tttttgtcgg taaaaaagtc acggtgaaag atggcaaatg ctacgatgaa
     1741 ttcggagcat tgggcggtgc ggcaatcacg atgatcgaat ccattcgcaa cgtagtgcaa
     1801 cattgcgata ttagcctgaa tgaagcaatc cgaatgagta ccctttaccc ggcaaaagcg
     1861 attggggtag aggaaacact cggctcactt gaagccggca aaattgccaa ccttgcgatt
     1921 ttcgataatc aatttgaagt aaaagcgact gctgtaaacg ggcaattaca gtggcattaa
     1981 ttttgctaca aattctaagc ataacagcat ataatccaaa ggcaggcatt gtgtctgcct
     2041 tttatttata gggagaataa catgcaattt aaagattact tagccacttt tccaactatt
     2101 gatcatctag ccggcttaaa cgtgataaat agcggtgaag tgattcaaca tattcctgcg
     2161 attgaaggta aattaggttc actaaaactt tacaatgcac tgtttgcgaa atttgatggg
     2221 aaattaaccg ctagtgcagc aaaacaaggg ttggaatggt ttgccgaaca cgttgaagat
     2281 gcgaaaaata atgtgggtaa gcacccgaat attgatctat tatttagggt aattgagcaa
     2341 gatttatctt taagtttaga gccgattgtt aaataagatt gcactagtga aaaataacaa
     2401 gcggtcatta tctgcaaaat ttttgcaaat aatgactgct tgtgtttaag aggcttattt
     2461 cacggtaatg cgtgcaaatt tgcgtttgcc gacttggtaa acaaaagtgc ctttttgagc
     2521 agtttgtttc acatcttcca ctttttcacc atcaatttta accccgcctt gttgggctga
     2581 gcgaattgcc tctgaagtgg acggtactaa gccggcttct ttcagtagag ccgctaggcc
     2641 gatttcaccc tcaaaggtaa attcaggcac ttcatcagga attgcccctt tttggaaacg
     2701 gttgataaac tcttgctctg ctgcatctgc tgccgcttca tcgtggaaac gagcgataat
     2761 ttcttttgcc agtagaattt tcacatcacg cgggtttttg ccattttcaa catccgcttt
     2821 taattgtgca atttcgtcta atggacggaa tgagagcagg ttgtaccaat cccacattaa
     2881 atcgtctgaa atcgacataa ttttgccgaa catttcgctt ggagcttcag ttacaccaat
     2941 gtagttgccg agagatttcg acattttttt ctcgccgtct aagccgacaa gtagtgggag
     3001 tgtcattgcc acttgtggtt tttgaccggc tgatttttgt aactcacgtc caactaacaa
     3061 gttaaaggtt tggtctgtac cacctaattc cacgtcagct ttcagttcta ccgagtcgtg
     3121 accttgtagt agcggataaa taaattcgtg gattgcgatc ggttgttggt tcgtgaaacg
     3181 gtttttgaaa tcttcacgtt ctaacattcg agcaacggtg tagttggagg ctaagcggat
     3241 cataccttcc gtacctaact tgcctaacca ttcggagttg aatacgatct tggttttttg
     3301 tggatctaaa attttgaaga tctgctcttt ataggtttcg gcattgcgta acacgtcttc
     3361 acggctaagt ggcggacggg tagagttttt tcctgatgga tcgcctacca taccggtgaa
     3421 atcaccgatt aagaaataga cttcgtgtcc gaattgttgg aattgacgaa gtttgttcag
     3481 cacaacagta tgacctaagt gaatatccgg tgcagttggg tctgccccta atttaatacg
     3541 caatgggcgg ttttctttta atttggtgat taaatcttct tctgaataaa tattttccac
     3601 gccacgttta agttcggcaa ggactgattc gatagcttga gtcatttatc ttccttttag
     3661 tcttgaatat aaaaaatgga tctattatag cggtatgaat tgaaattgat aggctatagg
     3721 cgaaaaaaaa cgccgtattt tgtgggggaa tacggcgtaa agagttggag tgattaccta
     3781 ttatttttga agtgatttaa tgataatgat tatcatctgt taagtcaata ctttttttga
     3841 aaattttcaa taagaattat tctcatcaat taagtaagat ggatactacg gcaattagtg
     3901 aagaatgtac agcggaattt tcgtattgta agagtgttgg taagcggtat ttttagaaaa
     3961 aacctccgag agcggttatt tttcggaggt tttttgcaaa tgaggattta catcgttgaa
     4021 atgtatttca acatcacccc cgctgcaatc gcagagccaa tcacgccggc aacgtttggg
     4081 cccattgcgt gcattaacaa gaagttttgt ttatcgtact cttgtccgac tttgtttgat
     4141 acccgtgcag ccatcggcac ggcagaaaca cccgctgagc cgataagtgg gttgattttg
     4201 tgctgactaa agcggttcat tattttcgcc ataatcacac cgcttgcagt gccgatccca
     4261 aaggcaatca cgcccaatac taaaatgcct agtgtttgcg gttgtaagaa tttgtctgcg
     4321 accaattttg cccccaccga taaccctaag aaaatcgtca cgatattgat taacgcattt
     4381 tgggcggtgt cgtttaagcg ttctactacg ccactggttc gcagtaagtt gccaaagcag
     4441 aacattccca ataacggtgc agcatccggc agtaagagtg ccactaataa tagcaacacg
     4501 atagggaaaa taattttctc acggttgctt acattacgga gctgaaccat tcggatttta
     4561 cgttcttgct cggtggttaa ggctttcata atcggcggct gaatcagtgg cactaacgcc
     4621 atataagaat aagccgctac cgcaatcgca cctaataatt caggggcaag tttgctggca
     4681 agataaatcg cggtcgggcc atctgccccg ccgataatcc caatcgcagc ggcttgtggc
     4741 aatgtgaagt cgataatgcc taaccagttt agccctaatg cacccagtac ggtagcaaaa
     4801 atcccaaatt gagccgcagc cccaagcatt aacattcgag ggttggcaag taaagggccg
     4861 aaatcagtca tcgccccgac acccataaag ataataagcg gagcaacgcc gtagccgata
     4921 gcaactttat agaataatgc caacacaccg gcggtatagc ccatatcgcc agccattact
     4981 tcaagctcat tttgaacaga gggagcagcc agtgcgatgg catctttaat cgaatgcaga
     5041 tcaaggcttg tactatttac cttagcagca ataattgcca gttgctcggc agaaccgctg
     5101 tggagtaaat tgtctaatgc cgtcattgcc atacctgctt ccggaatgtt tgacatcaaa
     5161 ccaccaaagc caataggcag tagtaaaagc ggttcgaatt ttcgggcgat ggcaagccaa
     5221 agcaacacta aactgatgac aatcataatc gcttgcccca gttctaaatg cattagcccc
     5281 atacctttaa aaagtgcaat aatactttcc ataccggact cctaggcaat actcattaaa
     5341 gtatcattta ccgccacaac atcgccttgt ttgacattaa tgccttgaat cgtgccggat
     5401 tttgccgcac aaatttgggt ttccattttc attgcttcaa gaatcagtaa cacatcgcct
     5461 tcagcaactt gttgcccctc tttcacttcc actttcaaaa tgttccctgc cattggagca
     5521 ttcaccggtt cggcattggc tgatagcgta tttgcagatt ttggtgcaga atcgaccgct
     5581 tgcggagccg gtgttgctgg tgttggagcg atattggtta tatcgccacc ttcactcacc
     5641 ttcaccacaa aggctttgcc ttcaagctca acggtataaa tggctgaacc ggattgtgtt
     5701 tgggcttttg gtgcagaagc agcggaggct tgtgccggtt ttgcttcatc agcacttggc
     5761 actggctcaa aggcagacgg attattgcgg ttttccaaat atttccagcc cacttgttgg
     5821 aagagtgcca caatcaatac atcatcaatc atgttttcag atagcgtaat gcctttttct
     5881 tttgcacttg cagtcacttc tgccactaat ttttccagtt ccggcggaag atgatcggct
     5941 gggcggtcgg taatcggctc tgctccctca agcactctgt cttgcaaggc tttgtttacc
     6001 ggtgccggtg tgcgaccata ttcgcctttt aaaatacctg cagtttcttt ggcgatagtt
     6061 ttataacgtt cgcccattaa tacgttcatc accgcttgtg tgccgacaat ttgtgaggtt
     6121 ggtgtcacca acggaatatt gcctaaatct tcacgcacac gaggaatttc ttgtaaaacc
     6181 aagtctaatt tatccgacgc attttgttgc ttgagttggc tttcgaggtt ggtcagcatg
     6241 ccacccggca cttgggcaac caaaatacgg ctatctatac cacggagctg tccttcaaat
     6301 tttgcatatt ttttacgcac gttacggaag taggcagaga ttttctccag agctggaatg
     6361 ctcaagccgg tttcatacgg cgtgccttga agtgtggcga caatagactc ggttgccggg
     6421 tgaccgtaag tgccggacat tgatgaaatg gaggtatcga caccatcgac accggcttca
     6481 atcgctttga gcaacgccat ttcagccata ccggtggttg aatggcaatg gagatgtagc
     6541 tcaacgtcgt agcgtttttt aatttcgctg actaattcat aggctgccat tggggttaaa
     6601 atgcccgaca tatctttgat caccaacgaa tccacgccaa tttccaataa ttgctcggtt
     6661 acatccaacc acgtttgtag ggtatgaaca gggctggtgg tgtaactcaa agtaccttgt
     6721 gcgtgcccgc cgtgtttttt gaccgcttgt aatgcggctt gcatattgcg agggtcgttg
     6781 agggcatcaa atacacggaa cacactcata ccgtttgcca ccgaacgctc gacaaattta
     6841 tctaccacat cgtcggcata atgacgataa cccaataagt tttgcccacg tagcagcatt
     6901 tgcattgggg ttttcggcat tgcttttttc agctcacgta aacgcaccca cggatcttcg
     6961 cctaaaaaac gaatacaact gtcaaaagtt gccccgcccc aagtttctaa cgaccaatag
     7021 ccgatattat ccagctcgga ggcaatcggt aacatatcgt ctaagcgtaa acgagtcgca
     7081 aaaagggatt ggtgtgcatc acgcaaaacc acatcggtaa tcgcaatttt tttcggttga
     7141 gttgtcataa tcaaattcct tctaattatt ttatttttga aatcttgtta atgttgcccg
     7201 ttaattcgac gatgatgagc aatcgcagcg gcaatcacag ggcgtaaacg ctctaaatca
     7261 tctgggggag attgaggcac agttttagag gctggaatag aaacaggttc ggggaaatat
     7321 tgattgataa tcttggaaat aaagctgatg gcataaatta aaaccagcag gaacagcata
     7381 acaaatccca ttccggctac cattaaattt ataccttccg caaagagttc tgcaggtgcc
     7441 ataatttatc cttaaaatgt agaaatgatg aagaaaatgt agaaaaaatt cagatttatt
     7501 tcaaattatt tagcctcaaa atgagtggta gatcacaaac tttaaacgca agcggtcaga
     7561 ttttacaaat tttttacaag agtagttaaa atttgcaaaa cctgacctta ggttagcttg
     7621 aagctaagtc gttatgccat tagccccgaa atatattttg aaagggtcat tgcacttagc
     7681 accgccatta gaatcaccac aatcgcccct gaaacggtca tccacacagg gtgtttgtag
     7741 tcgcccacaa tcttggtttt gtgagctgcc agcagaatta acccgaggga aatcggtaga
     7801 attaagccgt taagcgtccc tacaaacacc aagacttgtg ccggtctgcc gatggttgcc
     7861 agcacggcgg ttgaaaccac aataaaggcg acaatgaaac agtttttgtg tttttcgata
     7921 aatggcgaga atgaagtaat gaaggaaacc gaggtataag ccgccccaat cacagaggta
     7981 atggaggctg cccaaatcac cacgccgaag atcatcaaac cgacatcgcc ggcaacgtgg
     8041 gcaaatgggg tagccgcagg gtttttcgga tcaagttgca cgcccattga caccacgccc
     8101 agcaccgcta aaaacagtac cacacgcata attgaggcga ttaaaatggc tgacaccgag
     8161 cttttgctca cctcttttaa attctctttg cctttaatgc ctgcatcaag caaacggtgc
     8221 gcaccagcaa aggtgatgta tccaccaaca gttccgccca ctagcgtgac aatcgcaatc
     8281 acgtctaatt tttccggcac aaaggtatgc accaccgcat cgtcgattgg cagattggct
     8341 tttacggcaa cgaagatggt taaagcaatc atcacaaagc ccataatttg tgcaaatctg
     8401 tccatcactt taccagcttc atggaacagg aacacgccca cggcaatcgc accgctgacc
     8461 accgcaccca tttcagggga aagtccggtg agaatgttta gccctaaacc tgcaccgccc
     8521 acgttgccga tattaaaggc taaaccgccc atcacgataa gcagagccag aaagtaacct
     8581 gcccccggca atacggcatt ggcgatgtct tgagctttct tttccgatac tgctacaatt
     8641 cgccaaatat tcagttgagc accgatatca agcaagatcg agagtaagat cacaaagccg
     8701 aagctggcaa gtagggtttg ggtgaaggtg gctgtttggg ttaagaaccc cggcccgatg
     8761 gcggaggtcg ccattaaaaa tgctgccccg agtagggcgc tacggttgtt gtttgacatt
     8821 gtgttggttc cttttttgtt gttaatgagt cattattcgt ggatttatca tggtaatatt
     8881 cccctcttta gcaaagaggg gttaggggag atttggcagt agaactcatc gaagtgagag
     8941 agttaataat gctcaattta acctcttgaa attacttatt ttctccgtaa atcgcttctg
     9001 tcaaatctcc cccagcccct cttttctaaa gaggggagtt cgatctccaa gagttttatt
     9061 tctggttttc cgtccatttt gactaaaaca ggatcaggct ttcaccaaaa tcccctttgc
     9121 ctgtaactct tttttaattc gctcggcaaa taccacggcg tgttcgccgt caccgtgtaa
     9181 gcaaatgctg tcggctttaa tcgccacatt accaccatca acggttggta cagtgccgtc
     9241 taacaccatt tgcaagactt ggctgatcgc ttcatcatcg ctttctacca ttgcatcatc
     9301 acgggtgcga ggcacgagcg agccgtcagc taaatagtgg cggtcggcaa agacttcgga
     9361 aatggtttcc aaaccgtgct gctcggcaat gctcaacatc aggctgccgg ataagcccat
     9421 cagtttgagg ttgggatcga actgtttgac cgtattggcg ataagggtgg caaggtgagg
     9481 gtctttggcg gccatattgt ataaagcgcc gtgcggttta acgtagctca tcggcatatt
     9541 ttccgcatca cataaggctt tggcggctcc cagttggtag agcagacagg ctttaagttc
     9601 atcatcgggc agttgcatat tggtacgccc aaagttttcc ctatctggaa aactcgggtg
     9661 agcgccaact cgcacgctgt tttccttcgc ccagttaatc gctttacgca tttcattgta
     9721 atcaccggcg tgcaagccac agcagacatt cgccgaagta acgagctgta ataatgaatg
     9781 atcgttgcca caaccttcgg caagatcgac atttaaatca acgtgattca ttcgcttttc
     9841 tccgaattgt ttccaaataa ttttgatctt tttgataaat ttcgaccgct tgttcacggc
     9901 ttacttgctc aaattgcacc gttgagccga acggattttg tgccaaccgc ccgagatcgg
     9961 cctgaataat gcaggcaatt ttcggataac caccggtggt ttgggcatct gccatcagca
    10021 caatcggctg gccgtccggc ggcacttgca ccgtaccact cggggctgcg taggagagca
    10081 tttcaaggga agcggtcaat tccaacgctt tttctgcaaa acggtagccc atacggttgc
    10141 tgttgttttg cagttgccac ccttgttgcc aaaagcgttc acggctatct gcggtaaagg
    10201 cttcatattc gctcgaagcg gttgcccgaa tgcgtgaggt gaaatcaatc ggctcaatcg
    10261 acatcaaaga aagtcggctg tctctgcggc ctgtggcgat gttatcgccg gcttttaaca
    10321 ttctgccttg aaaaccgcca aattgagctt tgagagcggt acttgccgaa cctaacacct
    10381 gcggcaccag aaaaccgcct gccacgcata aataggcgta attgccgaga acaatccgtt
    10441 ttatcgtcag cgtttgacgg gcgttggcag tgtagcggta ataggaaaaa accggcgtgc
    10501 cgtccagctc cgcctccacc aatgcaccgg tgaggcaaaa tggcgtgtcg caatcaaagg
    10561 taatccgcat tccgccaagg gtaagttcaa tcgcaggtgc attatttgca ttttttaaca
    10621 ataaattacc cgcttgtaag gcaaggctgt ccatcgcacc gccgtggctc acgcctaaat
    10681 ggcgataacc ataacgccct aaatcttgaa tgtgggcaaa accctgtatt atttcgatat
    10741 aaatcatagc gttatactct ccgccacaaa tctaacatta tcgccggctg taagtagcgt
    10801 tggcggattg tgttgaatat caaacaacgg taactcggtt cgtcctaaca gttgccagcc
    10861 acctggtgag gtaaagggat aaatgcctgt ttgcctgccg ccgatgccga ccgaaccggc
    10921 agggactttg gtgcgaggca cgtttcggcg tggggtgtgt aaattttgcg gtaagccgtt
    10981 gaggtaagga aagccgggtt gaaagccgat cataaacacg gtgtaagtcg gttcggtatg
    11041 acggcggata atctcttcgg gagtggtatt gtggaattgg gcgacttcaa acagatcttc
    11101 gccaaattca ccgccgtaat ggacagggat ttcaatcagg cgtggggatt gtgcggcttg
    11161 ggcttggtcg ttttcccaga gttgttgaac cgcttggata aggcgtgtgc tatcggtgcc
    11221 aatggcgtga aatagggtaa aattgttcat tcctaccacc acttccaaca cgccatctaa
    11281 ggctcttgcc tgttctgcca cccgccaaat ctgttgctgt ttggcaagct ctgcaggcgg
    11341 ttcggctaag cagagcaggg aatgttcgct ggtatgatga atcgtcagca taattgccct
    11401 caaattgtta caaaaaggta acaacttttt acgctgatat atccagactg tcaattttat
    11461 tttgttgaaa atgtgaaata gctcttaatt ttcagaaatt tgatcatttc ttattcgcca
    11521 aaacgttgca aatctgggct tgcctgattc tgtgacacct cgatatttat aggtaatgat
    11581 tgagccaatc ggtggcggat tttctcgttc tttatcttta aaaccgctgc caatccgaaa
    11641 acgtccacgt tgattttcac aagtgattgc cccgagttta tcggtatatt tccctttgcc
    11701 tttatgatgg gcaaccacag tgcattcctc atctaaaacc ggtttgattt tcaaaatttg
    11761 ggcagaacga ccttgaatat agcctgcatt cgggttgcga acgataaccc cttcgccacc
    11821 ttgttccagc acagattggt aaaattgttg aaggtgggct ttatctttaa tcggaatttg
    11881 ctcaataata gcgatatgga gcgttggatg ttgcttcaga taattctcta aggtcgccag
    11941 ccgttcaatc aaattccctt gtgcatttgg cacatcgaac acctgtaatt tcagcttgtg
    12001 ccaacctttg ggctgagcgg aacgcacaat gcttgaaatt tcctcaaatt tgccccgttc
    12061 gctaaacagt tcgccgtcaa tcgcaaaagg agggaaatct tgaataaaat agtcgggtgg
    12121 attgagcgga taaccttggc ggctgatgag ctgttgaccg tcccaaaagc ctcgcacgcc
    12181 atcgagtttt tctgacatta cccagccact gatgtcttga tcttgatatt gctcaacgag
    12241 cattaactcg ggcgatttgg caaaaatagg gttaccaatg aagagtagga ataagagcag
    12301 ttttttcata gtctctccga aatagtgcct ccgaatatgc aaggcactat aaaggaaagc
    12361 gtgcaaacag tcagaaatga atctcaattt ttcgacgcag atcgcaaaat tttccgaaaa
    12421 actgatcgct tgagtgagaa atttaacgta ctgtttgaat taaagcagaa atatcctcgc
    12481 taaaggtttc caacttcgcc atttcttcaa tttggctatg agaatatttc ttcacattgt
    12541 aaacgaccac catacttaca tcacctactt gcaataaatt actttcgcca aaaggcgtgt
    12601 agcgtgttgc cccaatgctg ataatcgctt tagttgggta gtttgcctgt tttaacattt
    12661 cggaaatgtt gttcatcgga ccttcatcgg gctgattatt cattctctct acaatccaat
    12721 cgagcaattt ttgatggaaa tagctgtact ccaccgcagg gctgtcgatg ccgtaggtat
    12781 tcagctcgcc attacgttta tggaaacaag cgatgttgta attatccagc gtgccgcctt
    12841 tgttgaagtt atctaacgga agatgaattg ccgaaagccc tttagacgaa gccccccagt
    12901 ttttcttttc acagatttta ttcgcattcg gacggcggat agagcagtca ttgtaagccg
    12961 caaaatgggt tggcgtgagc gaaaccactt gctgatcttg gtagccaatt tcacaaatta
    13021 acgccacttc aggctcaatt tgcagattat cagcatcact cgggaaatta atcgcagtat
    13081 ttgaaagcgg ataggtggat aaaaactgat aatcccccaa attttccgcc ggcacataaa
    13141 acgggaaaat cgcctttggc tgcactgctt ccactgtttt cactttcgca aaatccgcct
    13201 cctctccggc ttgttctaaa tgtccagcaa aattgccggc cacgcctaaa ccgataaagt
    13261 gttccataat ctactcctta attggtaaaa gttggataaa attttaccgc tt
//
LOCUS       DANLXS010000060        13234 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.060, whole genome shotgun sequence.
ACCESSION   DANLXS010000060 DANLXS010000000
VERSION     DANLXS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.060"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..533
                     /locus_tag="PXD71_001243"
     CDS             <1..533
                     /locus_tag="PXD71_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291372.1"
                     /translation="WKYILPLIAFTALILFLAIVFPENRYVEHALEPVINLLSGTGKV
                     SSSTDNLVKNHLFLPTLNQFISGDGLYYTETGGYYGGSDSGYIRQVLYGGVSYMLACF
                     AFTYYFVRKVAVNWFEGSWIFTLSTIGILAILNIKADTFAFPGLMMLFLMFLSLFGTS
                     GRNVTFFCKNKEAEYV"
     gene            526..1491
                     /locus_tag="PXD71_001244"
     CDS             526..1491
                     /locus_tag="PXD71_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL6291373.1"
                     /translation="MFSIIVPSYNRNQEINALLESLKQQTAYNFEVIIVDDCSKIPVS
                     ETLGILNEFNDISTKEKVRELLTRDSLSHIYSYPFDVKVVRNNPNVGAAQSRNVGATN
                     ATQEWLLFLDDDDRFMPEKCERLAAEIANNPTANFVYHPAKCEMVNEGFTYVTKPFKH
                     KSELTKENILKANKIGGMPMIGLKKSLFCKIGGLSDRLRSLEDYDFLLKLLEEPSFEP
                     LYVDEALTYCTFHTKRSSVSTDTTNTEKAIEYIQQTYIKTPEANNFKLNSLYILSYPH
                     VMNLSRKAAGYYFEMFKQTKNIKQLIIALVIFISPKLAINLKRFM"
     gene            1491..2291
                     /locus_tag="PXD71_001245"
     CDS             1491..2291
                     /locus_tag="PXD71_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6291374.1"
                     /translation="MKFSVLMSLYIKEQPQYLRECFESLKVQTVQADEIVVCFDGAVT
                     PELEAIVEEYSAILPINAVKFPQNRGLGKTLNDGLTYCKNEWVFRMDTDDICMPERFE
                     KQVAFIEQNPETVIFGGQIAEFGQDIHDLVAYRNVPTTVEEIVRFTRLRCPFNHMTVA
                     YQKSKVLEVGGYQDLQEDYYLWIKLIAQFKQVANLPDLLVYARVGNGMVGRRRGMAQA
                     EAEWRLYKLKNRCQIHDPISGFGIFIMRAIPRLLPVSALKKVYGLLRK"
     gene            complement(2328..2834)
                     /gene="msrA"
                     /locus_tag="PXD71_001246"
     CDS             complement(2328..2834)
                     /gene="msrA"
                     /locus_tag="PXD71_001246"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL6291375.1"
                     /translation="MQNIREIYLAGGCFWGVESFMQRIKGVIKAQSGYANGNTDNPSY
                     QEVCAGSGHAEVVKVTYDANQISLAKLLDYFFKVIDPVSINKQGGDIGIQYRTGIYYT
                     DPQDEPVVTAALAQLQTQFDQPLAVENQMLEQYFPAEEYHQEYLVKNPNGYCHIDIQL
                     MNEILKNQ"
     gene            3015..4277
                     /locus_tag="PXD71_001247"
     CDS             3015..4277
                     /locus_tag="PXD71_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL6291376.1"
                     /translation="MGTLNSFLGIIVLLFIAFLFSSNKRAINVRTVLGALALQVAIGA
                     LVLYVPAGRDALNAMATGVSKVISYGNEGISFLFGGLVSDKMFEVFGGGGFVFAFRVL
                     PVIIFFSALISLLYYIGVMQWVIRILGGLLQKALGTSKAESMSAAANIFVGQTEAPLV
                     VKPYISKMTESELFAIMAGGLASIAGSVMAGYAGMGVPLTYLIAASFMAAPAGLLFAK
                     IMVPQTEKFRDELEAVDLEKPANILDAAASGASSGMQLALNVGAMLIAFVALIALVNG
                     ILGGIGGWFGYDTLNLGQIFGWVFKPLAWVIGVPWEEAEIAGQMIGLKLAVNEFVGYL
                     EFAKYLAPESTVHLTDKTVAIITFALCGFANFSSIAILIGGIGAMAPSRRGDIARLGI
                     KAVIAGSLANLMSATIAGLFIGLGGAVL"
     gene            4422..5198
                     /gene="deoC"
                     /locus_tag="PXD71_001248"
     CDS             4422..5198
                     /gene="deoC"
                     /locus_tag="PXD71_001248"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL6291377.1"
                     /translation="MNLKKSAEIALSLMDLTTLNDNDTDEKVIALCQQANTKFGTPAA
                     ICIYPRFIPIARKALKQQGIEQVKIATVTNFPHGNDDIEIAVAETKAAVAYGADEVDV
                     VFPYKALIAGNEQIGFDLVKQCKAVCAANNVLLKVIIETGELKSAELIRKASEISIQA
                     GADFIKTSTGKVAVNATLESARIMLETIRDLGVADRVGFKAAGGVKTSEEAAQYLALA
                     ESILGNDWVSASHFRFGASSLLANLLATLNDEQQQAVQGY"
     gene            5219..5938
                     /gene="deoD"
                     /locus_tag="PXD71_001249"
     CDS             5219..5938
                     /gene="deoD"
                     /locus_tag="PXD71_001249"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL6291378.1"
                     /translation="MATPHINAPEGAFADVVIMPGDPLRAKYIAETFLEDAKEVTNVR
                     NMLGYTGTYKGRRISVMGHGMGIPSCSIYAKELITEYGVKKIIRVGSCGAVRNDVKVR
                     DIVIGVGACTDSKVNRIRFRDNDFAAIADFDMTLAAHQAAKAKGIEARFGNLFSADLF
                     YSPDVEMFDVMEKYGILGVEMEAAGIYAVAAEYGAKALAICTVSDHIRTGEQTTSEER
                     QLTFNGMIEIALESVLIGDKQ"
     gene            complement(6014..6865)
                     /locus_tag="PXD71_001250"
     CDS             complement(6014..6865)
                     /locus_tag="PXD71_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL6291379.1"
                     /translation="MQTHRAIIESIFALAPKNELWATPISGLTVHAADRSIRFNGYIQ
                     DPSLCVVLLGERQICTNGHCQVFGNQEMMFCPVNLPIVTQTLSVAENQPYLAISLRLD
                     FDLIAKIITQLPPQKISENINQRTKWELENEIKDCLLRLLNLLNSPNHIHFLAPLILN
                     ELYFYLLQSEQGGYIRSLLSQDGNIAKIANATKILERKFADTLNMSALAEEVGMSVAG
                     FYAHFKSVTSMTPLQYQKSLRLNAAREKLQAGVSVSETAYQVGYESPSQFSREYKRQF
                     GESPKGR"
     gene            6980..7411
                     /locus_tag="PXD71_001251"
     CDS             6980..7411
                     /locus_tag="PXD71_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL6291380.1"
                     /translation="MKIFYHTSATATGGRDGRTQVDDGSIGFDLVGFQNESGKVGTNP
                     EQLFAMGYAACFDSAMNHVAPTLGIKPTKSSTTVAVGIGQKADGAFGLDLDITITVEG
                     ISAEEAKTLISKAHEVCPYSNATRGNVDVRLHVKVVSEFDL"
     gene            7428..8108
                     /locus_tag="PXD71_001252"
     CDS             7428..8108
                     /locus_tag="PXD71_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDL6291381.1"
                     /translation="MKMLDFETTVKTRQSIRKFLPLPMTETEIKQILADAQNAPSACN
                     TQPWLVHVASGETLARLKARFKKTFEAGINDADFEFNQTKFTGDYEPRWRNQYSHVFT
                     TSYGIPREDKAGRQVVYDDNIVGYGAPHMAFLFMPSIDGHDVNIASDVGMYSQTFLLS
                     LTARGFGGIPQLALAMFAKDVREELGIPEHYKLLHAISFGHPDWDAMQNKHHLGRVPV
                     EESATLHW"
     gene            8172..9296
                     /locus_tag="PXD71_001253"
     CDS             8172..9296
                     /locus_tag="PXD71_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL6291382.1"
                     /translation="MINLFTPYTLNNDVEIKNRLVVAPMTHSASNDDGTISEQERHFL
                     HNRAADMGLFITAATGVMRNGKAFYGQPEALDERYLASLTETANILKNQGTKAILQIF
                     HGGAQAIPALLDGANAVAPSEHANGAKALTADEVKQMIQAFANATDLAIQAGFDGVEI
                     HGANGYLIQQFFSTEFNQRTDEWGGSLEKRLRFPLAVVEAAVSVREKHQRPDFIIGYR
                     FSPEEPGEHGLTMTDTYALIDALCEKPLQYLHISLWDFGKKARRGADTNFTRMQLVHQ
                     HINGRLPLIGVGNLLTGEQIRKAYATGWAEFIALGKAVMLNPNVATLLKDNRDSEIVS
                     ELDPQQADHYGIPDRLWGYCVQGGAWLPPLKGQNGQPMDI"
     gene            9380..9997
                     /locus_tag="PXD71_001254"
     CDS             9380..9997
                     /locus_tag="PXD71_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDL6291383.1"
                     /translation="MITLHYLNSSCSHRIVWLLEALGLDYELKIYHREESGFAPMEVK
                     ALHPLGKAPVLQDGDLVLAEGNAIIQHLIARYAEGKFEPTRGSEAYSMYHYWLSISAS
                     LFSANLVTMLANRSDLGAFGDYAKAQTPLYFNHIEQTLSDKKWIVGDELTGADFSLCF
                     PLQWGRNFVEMANYPNIARYLEQVENHPAYQKANQKALKLDMNRF"
     gene            10140..11081
                     /gene="argC"
                     /locus_tag="PXD71_001255"
     CDS             10140..11081
                     /gene="argC"
                     /locus_tag="PXD71_001255"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL6291384.1"
                     /translation="MSKYKIFVDGAVGTTGLRIHERLSQADDIELLTLPEEQRKDLNA
                     RVAKVAEADLTFLCLPDEASKELVANAPENTRICDTSTAHRVNPNWVYGFPELSGQRE
                     KIQNAKRVAVPGCHATGYIALVRPLVEKGILPADYPLSCHSLTGYSGGGKAMIADYEN
                     ENSPNAFNAPRVYGLTLQHKHLPEMQAVTQMQNPPIFTPVVADYYSGMLVTVPLQIFA
                     LQAVKSGEEIANLLADYYQTSRLIHVHPFQSLPEDGMLAANAYAGKDSLEIFIYGNST
                     QLLLAARFDNLGKGASGAAVQCMNIMLGRDEVEGLEL"
     gene            11173..12027
                     /gene="argB"
                     /locus_tag="PXD71_001256"
     CDS             11173..12027
                     /gene="argB"
                     /locus_tag="PXD71_001256"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDL6291385.1"
                     /translation="MQQQEINQFANLLEKSTACLVKWQGKIIVVKYGGNAMINEELKK
                     SVMADLLLLHRLGVKVVLVHGGGLEISQGVKLLGKEPQFINGLRVTDSDTINVVLQML
                     AGKVNKSLVALLKGKGIGLCGIDGAMLQCKKLEAEVDYGFVGDIVKVDPTVIEIALNA
                     GLIPVVSTVGVDENGQAYNINADTAASEIAIALQAEKLVSMTDIAGLLRDRFDESTLI
                     PEVEVSEVQSLIEQGIIAGGMIPKIACCTDFIRAGGSEANIIDGRIDHAILVEMFGGK
                     NGTRFYKK"
     gene            12040..13224
                     /locus_tag="PXD71_001257"
     CDS             12040..13224
                     /locus_tag="PXD71_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDL6291386.1"
                     /translation="MTSSNTIKQLDKDYIAQTYGRFDLALSHGKGCEVWDFEGNKYLD
                     FTSGIGVNSLGWADEDWLQAVTTQAGKLAHTSNLFFTEPASQLAQKLVAASGLKRAFF
                     CNSGAEANEGAIKTARKYSFDKYGSGRSTVLTLVNSFHGRTISTLASTGQEVFHKHFF
                     PFTEGFEYLPVNGIEALKARLETENVCGILLEVVQGEGGVCPLEADYLHAVQSLCQEK
                     DILFMIDEVQTGIGRTGTLFAYQQFDLTPGLVTLAKGLGGGLPIGAFLLGEKVEMTLG
                     KSDHGSTFGGNPVSCAAANVVIDKINAEFLAQVQQKGQLLQQRLASLPKVKSVSGLGL
                     MLGVEFEEGILAADIVAKCINKGVLFLTAKSKLRLLPPLMISEQQIEQGIRVLEDVLK
                     DS"
BASE COUNT         3804 a         2571 c         3013 g         3846 t
ORIGIN      
        1 aatggaaata tatattgcca ctgattgcct tcactgcgtt aattctattt ttggcgattg
       61 tttttccgga aaaccgttat gttgaacacg ccttagaacc ggtaattaat ttattgtccg
      121 gcaccggaaa agtcagctct tcaaccgata atttagtgaa aaaccatctg tttttaccga
      181 ctttaaatca gtttatttcc ggtgatggtt tatattacac cgaaactggt ggctattatg
      241 gtggatcgga ttccggctat attcgccaag tattatatgg tggagtaagt tatatgttgg
      301 cgtgttttgc cttcacttac tattttgtac gcaaagtggc ggtgaattgg tttgaaggca
      361 gttggatttt taccttatcc accatcggca ttttagctat tttaaatatt aaagcagata
      421 cctttgcttt cccggggctg atgatgctgt ttctaatgtt cttgtcgctg tttggaacaa
      481 gcggtcgaaa tgtaacattt ttttgcaaaa acaaagaggc tgaatatgtt tagtattatt
      541 gtgccttctt ataaccgtaa tcaagaaatc aatgcgttgc ttgagagttt aaagcaacaa
      601 actgcatata attttgaagt gatcattgtt gatgactgct caaaaatacc ggtcagtgaa
      661 actttaggaa ttttaaatga atttaacgat atttctacta aagaaaaagt aagagaatta
      721 cttactagag atagtttatc tcatatttat agctatccat ttgatgtgaa agtggtgcga
      781 aataacccaa atgttggggc ggcacaaagc cgaaatgtag gggcaactaa tgccacacaa
      841 gaatggctac tgtttttaga tgatgatgac cgctttatgc ctgaaaaatg cgaacgttta
      901 gcggcagaga ttgcgaacaa tccaactgcc aattttgtgt accacccggc aaaatgtgaa
      961 atggtaaatg agggttttac ttatgtcacc aagccgttta aacataaaag tgagctaact
     1021 aaagaaaaca ttctaaaagc gaataaaatt ggcggaatgc cgatgattgg cttgaaaaaa
     1081 tcactctttt gtaaaattgg tggattatct gaccgcttgc gatcacttga agattatgat
     1141 ttcttactga aattattgga agaaccgagt tttgagccat tgtatgtgga cgaagccttg
     1201 acctattgta ctttccatac caaacgctcc agtgtttcaa ccgataccac taatacagaa
     1261 aaggctattg agtatattca acaaacgtat atcaaaacac ctgaggcgaa taattttaag
     1321 ctcaattcgc tttatatttt aagttatcca cacgtaatga atttatcccg caaggcagcc
     1381 ggttattatt ttgagatgtt taaacagact aaaaatatca aacaactgat tattgcgttg
     1441 gttattttta tttcgccgaa gttggcgatt aatttgaaaa ggtttatgta atgaaatttt
     1501 ccgttttaat gtcgttatac attaaagagc aaccgcaata tttaagagaa tgttttgaaa
     1561 gcttgaaagt acaaactgtg caggctgatg aaatcgttgt ttgttttgat ggtgcagtta
     1621 cgcctgaatt agaggcgatt gtagaagaat atagtgcaat tttgccgatt aatgcggtga
     1681 aattcccaca aaatcgaggt ttaggtaaaa cgctaaatga tgggctaacc tattgtaaga
     1741 atgaatgggt tttcagaatg gatacagacg atatttgtat gccggaacgt ttcgagaaac
     1801 aagtggcatt tattgaacag aatcctgaaa ccgtgatttt tggcggccaa attgctgaat
     1861 ttgggcaaga tattcacgat cttgtcgctt accgcaatgt accaaccacg gtggaggaaa
     1921 tcgtcagatt cactcgcttg cgttgcccgt ttaaccatat gacggttgct tatcagaaat
     1981 ccaaagtgtt ggaagtcggt ggctatcaag atctgcaaga agattactat ttatggatca
     2041 aactcattgc tcaatttaaa caagtggcaa acttgccgga tttattagtg tatgcccgag
     2101 tcggtaacgg aatggttggc agacgccgtg gaatggccca agcagaggca gaatggcgtt
     2161 tatataaatt aaaaaaccgc tgccagattc acgatccgat ttcaggtttt gggattttca
     2221 taatgcgtgc aattcctcgt ttattaccgg tttcagcact gaaaaaagtg tacggtttgc
     2281 tcagaaaata aagctaaaaa aaccacagat tgctctgtgg ttttttgtta ttgattcttc
     2341 aaaatctcat tcattaattg aatatcaata tggcagtagc cgttcgggtt ttttactaaa
     2401 tattcttggt ggtactcttc ggcagggaaa tattgctcta acatttggtt ttccactgcc
     2461 agcggctgat caaattgggt ttgtaattgt gcaagtgcgg cggttaccac cggttcgtct
     2521 tgtgggtcgg tataataaat accggtgcgg tattgaatac cgatgtcgcc accttgtttg
     2581 ttaatgctca ccgggtctat tactttaaag aagtaatcta gtaattttgc caagctaatt
     2641 tgattggcat cataagtcac ttttaccact tcagcgtgtc cgctaccggc acacacctct
     2701 tgatagcttg ggttatcggt attaccgttg gcatagcctg attgtgcctt aatgacacct
     2761 ttaatccgtt gcataaaaga ttccacgccc cagaaacagc caccggctaa gtaaatttca
     2821 cgaatatttt gcatattacc tctctataat tgtaaatttt tcgataaatt tgactgcttg
     2881 tgatgaataa taaaatacca taatttagtt tttgtcaaat tttgtcttga atgatgataa
     2941 tttcacaaga ttttttgcct aaaaataggt atagtatcgt cacctttttt aatcaattta
     3001 taattagagg ttctatgggt actcttaaca gctttttagg cattattgtt ttactattca
     3061 ttgcattttt attttcgagc aataaacgtg caataaacgt gcgtaccgta ttaggcgcat
     3121 tagcattgca ggtagctatt ggtgcattgg tgctttatgt gccggcaggg cgtgatgcct
     3181 tgaatgcgat ggcaacaggt gtgtcaaaag tgatttctta cggtaatgaa gggatttcat
     3241 ttttattcgg tggcttagtc agcgacaaaa tgtttgaagt atttggcggc ggcggctttg
     3301 tgttcgcttt ccgtgtactt ccggtgatta ttttcttctc tgcactcatt tctttgcttt
     3361 actacattgg agtgatgcaa tgggtaatcc gtattttagg tggtttatta caaaaagcat
     3421 taggtacatc taaagcggaa tctatgtctg cggcagctaa tattttcgtt ggccaaactg
     3481 aagctccatt ggtagtaaaa ccttatatca gcaaaatgac agaatctgaa ctctttgcga
     3541 ttatggcagg gggcttagct tctattgcag gttctgttat ggctggttac gcaggaatgg
     3601 gcgtaccatt aacgtactta atcgcagcct cctttatggc ggctccggca gggttattat
     3661 tcgccaaaat tatggtgcca caaaccgaaa aattccgtga tgaactagaa gcggtagatt
     3721 tagaaaaacc ggcaaatatt cttgatgcag cagcttcagg tgcttcttca ggtatgcaac
     3781 tggcattaaa cgtaggggca atgttaattg cattcgtggc gttaattgca ttagtgaatg
     3841 gtattttagg aggtattggc ggctggttcg gttatgatac cttaaactta ggtcaaattt
     3901 tcggctgggt atttaaaccg cttgcgtggg taatcggtgt gccttgggaa gaagcagaaa
     3961 ttgcggggca gatgatcggc ttaaaattag cggtaaatga attcgtgggt tatttagagt
     4021 ttgctaaata cttagctcct gagtcaaccg tacatttaac ggataaaact gttgcgatta
     4081 ttacctttgc actttgtggc tttgcgaact tcagctcgat tgcgatttta atcggtggta
     4141 tcggggcaat ggctccaagc cgtcgtggtg atattgcccg acttgggatt aaagccgtca
     4201 ttgctggctc acttgccaac ttaatgagtg caaccattgc cggcttgttt atcggcttgg
     4261 gtggagcagt tctctaatcc agtttgtata aaaccactag aaatagtggt tttatctttt
     4321 tttatctgac gtaaacggtt gcttttggtt tgtctattta caagcggtca gatttttata
     4381 aaattttacc aaccaccacg catttaataa aaggagacac tatgaattta aaaaaatccg
     4441 ctgagattgc tctttcgttg atggatctca ccacattaaa tgacaatgat actgatgaga
     4501 aagtcatcgc attatgccaa caagcgaaca ccaagtttgg cacaccggca gcaatttgca
     4561 tttatccacg ttttatcccg attgcccgca aagcattgaa acagcaaggt atcgagcaag
     4621 taaaaattgc gacagtcacg aatttccctc acggtaatga tgatattgaa attgcggtag
     4681 ctgagaccaa agccgcagtt gcttatgggg cagatgaggt agatgtggta ttcccttata
     4741 aagcattaat tgcaggtaat gagcaaatag gtttcgactt ggtaaaacaa tgcaaagcag
     4801 tttgtgctgc caataatgtg ttgcttaaag tgattattga aaccggcgag ttaaaatcag
     4861 cagagcttat tcgcaaagca agcgaaattt cgattcaagc aggggctgat tttattaaaa
     4921 cctcaaccgg caaggtggca gtgaatgcta cccttgaatc tgcccgcatt atgcttgaaa
     4981 ccattcgtga tttaggtgtg gcagaccgtg ttggctttaa agctgcaggt ggggtaaaaa
     5041 cctcggaaga agccgcccaa tatcttgcac ttgcggaatc tattttaggc aatgactggg
     5101 tgagtgcatc acattttcgc ttcggtgcat caagtttatt agctaattta ttagcaacat
     5161 tgaatgatga acagcagcaa gctgttcaag gttattaatt aataaagagg aacataaaat
     5221 ggctactcca cacattaacg cacctgaagg tgctttcgct gacgttgtaa ttatgccggg
     5281 cgatccgctt cgtgctaaat atatcgctga aaccttttta gaagatgcaa aagaagtgac
     5341 caatgttcgc aatatgttag gctacaccgg cacttataaa ggtcgccgca tttcagtaat
     5401 ggggcacggt atggggattc catcttgctc gatttacgct aaagaattaa ttactgaata
     5461 tggcgtgaag aaaattatcc gtgtcggctc gtgcggtgcg gtacgtaatg atgtcaaagt
     5521 gcgtgatatt gtcatcggtg ttggggcttg taccgattct aaagtaaacc gtattcgctt
     5581 ccgtgataac gattttgcgg cgattgctga ttttgatatg acattagctg ctcaccaagc
     5641 agctaaagcg aaaggcattg aagctcgttt cggtaattta ttctcagccg atttattcta
     5701 ctcacctgat gtcgaaatgt ttgatgtaat ggaaaaatac ggcattttag gcgtagaaat
     5761 ggaagcagca ggcatttatg cggtagcggc agagtatgga gcaaaagcat tagcaatttg
     5821 caccgtttca gaccatatcc gcaccggtga gcaaactacc tctgaagaac gtcaattaac
     5881 ctttaatggt atgattgaaa ttgcgttaga gtcagtatta attggcgata agcagtaatt
     5941 tctaacctga caagcggtga aattttgcaa aaaatttgca gaatttgacc gcttgttatt
     6001 tttgattctt cctttatctt cctttcgggc tttcgccaaa ctgcctttta tattctcgac
     6061 taaactggct tgggctttca tagccgactt gataggcggt ttcactcacg cttactcctg
     6121 cttgtagttt ttcccttgcc gcatttagtc ttaacgattt ctgatattgc agtggtgtca
     6181 ttgaagttac gcttttaaaa tgagcgtaaa accccgccac gctcattcct acctcttcag
     6241 ccagtgctga catatttagt gtatcggcaa attttctttc taagattttt gttgcattgg
     6301 caattttagc aatattgcca tcttgagaga gtagagagcg tatatagcca ccttgctcag
     6361 attgtaataa atagaaatag agttcgttta atatcaatgg tgcaagaaaa tgaatatgat
     6421 ttggagaatt aagcaaattt agtaagcgta acaaacagtc tttaatttca ttctccagct
     6481 cccatttagt gcgttgatta atgttttcac ttattttctg tgggggaagc tgcgtgataa
     6541 ttttggcaat aagatcaaaa tccaagcgta gtgaaatcgc aagataaggc tgattttctg
     6601 caactgataa ggtttgggta acaatcggca aattcacagg gcaaaacatc atttcctgat
     6661 taccgaagac ttggcaatgt ccattggtac agatttgccg ctcgcctaat aatactacac
     6721 ataaacttgg gtcttggata taaccgttaa atctaataga acgatcggca gcgtgaacgg
     6781 ttagccctga aatgggagta gcccaaagtt catttttcgg agcgagggca aaaatagatt
     6841 caataatagc tcgatgagtt tgcataataa tctcactttt agaaaattag gcatatttta
     6901 tagaaaatca ggcaagacga caaatagcaa aaagcggata atatccccaa tcaaataaag
     6961 catttaatag gagcaaaata tgaaaatttt ttatcacaca tcagcaactg caaccggtgg
     7021 tcgtgacggc agaacccaag tggatgacgg ttctatcggt tttgatttag tcggtttcca
     7081 aaatgaaagt ggcaaagtag gcactaatcc ggaacaatta ttcgcaatgg gctatgctgc
     7141 ctgctttgac agtgcaatga atcacgttgc cccaacctta ggcattaagc caaccaaatc
     7201 cagtaccact gttgcagttg ggattggtca aaaagctgat ggtgctttcg gcttagattt
     7261 agatattacc attactgtgg aaggcattag tgcggaagaa gcgaaaactt taattagcaa
     7321 ggcacacgaa gtttgcccat attcaaatgc aacccgtggt aatgtagatg tgcgtttaca
     7381 cgttaaagtc gtgagtgaat ttgacttata aaaaataaat aggggaaatg aaaatgttag
     7441 attttgaaac cacggtaaaa acacgacaat caatccgcaa gtttttaccg cttccgatga
     7501 cggaaactga aatcaaacaa attcttgccg atgcacaaaa tgccccttcg gcttgtaata
     7561 cacagccgtg gttagtacac gttgcctctg gtgaaacgct agcacgctta aaagctcgtt
     7621 ttaaaaagac ttttgaagcg ggcattaatg atgcggattt tgagttcaat caaacgaaat
     7681 ttaccggtga ctacgaacca cgttggcgta accaatacag ccacgttttt accaccagct
     7741 atggcattcc ccgtgaagat aaagccggtc gccaagtggt gtatgatgac aatattgtcg
     7801 gttatggtgc gccacatatg gcattcctat ttatgccaag cattgacgga cacgatgtca
     7861 atattgccag cgatgttggt atgtattcgc aaactttctt gctctcactc accgctcgtg
     7921 gttttggcgg cattccgcag cttgcccttg cgatgtttgc taaagatgtg cgtgaagagt
     7981 taggcattcc tgaacattac aagctcctcc acgccatttc tttcggacac ccagattggg
     8041 acgctatgca gaacaaacac catttagggc gtgtgccggt ggaagagagt gcgaccttac
     8101 attggtaata aacaagcggt tgatttttcg caaaaatttg cgaaatcaac cgtatttttt
     8161 aggagaatac gatgataaat ttatttaccc cttatactct gaataacgat gtagaaatta
     8221 aaaaccgcct tgtagttgcc ccaatgaccc attccgcctc caacgatgac ggcacaatta
     8281 gcgagcaaga acgccatttc ttgcataatc gtgcagcaga tatgggctta tttatcaccg
     8341 cagcaaccgg ggtaatgcgt aacggcaaag cgttttacgg tcagccggaa gctcttgatg
     8401 aacgctattt ggcaagcctg acagaaactg ccaatatctt gaaaaatcaa ggcaccaaag
     8461 cgattttgca aattttccac ggtggggcac aagctattcc tgccttgctt gacggggcga
     8521 atgcagttgc gccgtccgag cacgccaacg gtgctaaagc cctaacggcg gacgaagtga
     8581 agcaaatgat ccaagctttc gctaatgcca ccgatttagc tattcaagcc ggttttgatg
     8641 gtgtggaaat tcacggggcg aacggctatt taattcaaca atttttctct actgaattta
     8701 atcaacgcac tgatgaatgg ggcggtagcc ttgaaaaacg cttgcgtttc ccgttagcgg
     8761 tggttgaggc agcggtcagc gtgcgtgaaa aacaccaacg ccctgatttt attatcggct
     8821 accgtttctc accggaagag ccgggcgaac acggcttaac catgaccgat acctacgccc
     8881 tgattgatgc tttgtgcgaa aagccgttgc aatacttgca catttcactc tgggatttcg
     8941 gcaaaaaagc ccgccgtggt gcagatacca acttcacccg tatgcaactg gtacaccaac
     9001 acatcaacgg gcgtttaccg ctgatcggcg tgggtaactt attaaccggt gaacagatcc
     9061 gtaaagctta tgcaacaggc tgggcagaat ttatcgcctt aggtaaagcg gtaatgctca
     9121 acccgaacgt ggcgaccttg ttgaaagata atcgagacag tgagattgtc agcgaactcg
     9181 acccgcaaca agccgaccac tacggcattc ccgatagact atggggctac tgcgtgcaag
     9241 gtggtgcttg gttaccgccg cttaaagggc aaaacgggca accaatggat atttaatcat
     9301 tacagacaag cggtcatttt tttgtaaaaa tttgcaatta agtgaccgca ttttttcaat
     9361 tatctgaagg agaaaaatta tgattacgtt acattacctg aacagttcct gctctcaccg
     9421 catcgtttgg ttgttggaag ccttaggctt ggattacgag ctaaaaatct accatcgtga
     9481 agagagcggt tttgcaccta tggaggtaaa agccttgcac ccacttggca aagcccctgt
     9541 gttgcaagat ggcgatttgg tgcttgccga aggcaatgcg attatccagc atctgattgc
     9601 ccgttacgcc gaagggaaat ttgagccgac tcgtggtagc gaagcctatt caatgtatca
     9661 ctactggttg tcgatttctg cttcattatt ctcggcaaac cttgtgacaa tgttggctaa
     9721 tcgctctgat ctcggagctt ttggcgacta cgcaaaagcg cagactccgc tgtattttaa
     9781 ccatattgag caaacacttt cagataaaaa atggattgtg ggtgatgaac taaccggtgc
     9841 ggatttttct ctctgtttcc cactacaatg ggggcgtaat tttgttgaaa tggcaaatta
     9901 cccgaatatt gcacgctatt tagaacaggt ggaaaatcac cctgcttacc aaaaagcgaa
     9961 tcaaaaagcc ctgaagcttg atatgaatag attttaatac tctttttaat ccctaacatt
    10021 ttgttaggga tttttgaata taaaatcaat taatgtgaat aattattcaa aaataaactt
    10081 gagtttagcc ttaaatagcc ttagactacc gagcagaaaa agtcaaagaa ggattttcaa
    10141 tgagcaaata caagattttt gttgatgggg cagtaggaac aaccgggcta cgcattcacg
    10201 aacgtttaag ccaagcggac gatattgaat tattaacctt gcccgaagag cagcggaaag
    10261 atttgaacgc ccgtgtggca aaagtcgcag aggcggattt aactttcctc tgtttgccgg
    10321 atgaagcctc aaaagaatta gtagcaaatg caccggaaaa tacccgaatt tgcgatactt
    10381 caaccgctca tcgggtcaat cctaattggg tttatggttt tccggaattg agcggacaac
    10441 gtgaaaaaat ccaaaatgcc aagcgtgttg cggtgccggg ttgccatgcc acgggctata
    10501 ttgcgttggt tcgtccactg gtggaaaaag ggattctgcc cgccgattac cctttaagtt
    10561 gtcattcctt aaccggctat tcaggcggtg gcaaagcgat gattgctgac tatgaaaatg
    10621 aaaacagccc aaatgctttc aatgcccctc gtgtttacgg tttaactctc caacacaaac
    10681 atttgcctga aatgcaggca gttacccaaa tgcaaaaccc accgattttc acccctgtgg
    10741 ttgctgatta ttacagcgga atgttagtca ctgtgccatt gcagatcttc gccttacaag
    10801 cggtcaaatc cggcgaagaa attgcaaatt tgctcgccga ttactaccaa accagccgac
    10861 taattcacgt tcaccctttc caatctctac ccgaagacgg aatgcttgcc gcgaatgctt
    10921 atgcaggtaa agacagctta gagattttta tttacggcaa cagcacccaa cttttacttg
    10981 ctgcccgttt tgacaattta ggcaaagggg catcaggtgc cgcagtgcag tgtatgaata
    11041 ttatgttggg aagagatgag gttgaggggt tagagcttta aatataaatg tagggacagg
    11101 tcctgtgcct gtccgataat catcgcccat attcggacgg gcacaggacc cgtccctaca
    11161 agagaaagaa atatgcaaca acaagaaatc aatcaatttg caaatttatt agaaaaatcc
    11221 accgcttgtt tggtgaaatg gcagggtaaa atcattgtgg tgaaatatgg cggtaacgca
    11281 atgattaatg aggaactgaa aaaatcagtg atggcagatc tgctgttact tcatcgttta
    11341 ggcgtaaaag tggtactggt acacggtggc gggctggaga tttcacaagg cgtgaaatta
    11401 ttgggtaaag aaccgcaatt tatcaacggg ttgcgtgtta ccgatagcga tacgattaac
    11461 gtggttttgc aaatgttggc aggcaaagtt aataaaagcc ttgtcgcctt attaaaaggg
    11521 aaaggcattg ggctgtgtgg tattgatggg gcgatgttgc aatgcaaaaa attagaggca
    11581 gaggtggatt acggctttgt gggcgatatt gtgaaagtcg atccaactgt gattgaaatt
    11641 gctcttaatg cgggcttgat tccggttgtt tccacggtgg gcgttgatga aaacgggcag
    11701 gcttataaca ttaatgcaga caccgctgcc agcgagattg cgattgcgtt acaagcggaa
    11761 aaattggtct cgatgaccga tattgcaggt ttattgcgag atcgctttga tgaaagcacc
    11821 ttaattccgg aagtggaagt aagcgaagtg cagtccctga ttgagcaagg cattattgcc
    11881 ggtgggatga tccccaaaat tgcgtgctgt accgatttca tcagagctgg tggctctgag
    11941 gcaaatatta ttgacgggcg aatcgaccac gccattttgg tagaaatgtt cggtggcaaa
    12001 aacggcacac ggttttataa aaaataacag gaaagtgaaa tgacaagcag taatacgatt
    12061 aagcaattag ataaggatta tatcgcccaa acctatggca gatttgacct tgcactgtcg
    12121 cacggaaaag gctgtgaagt gtgggatttt gagggcaata agtatcttga ttttaccagt
    12181 ggcattgggg taaatagttt aggctgggcg gatgaggatt ggctacaagc tgttaccaca
    12241 caagccggca aattagctca cacctcaaac ctgtttttta ccgaaccggc aagccaatta
    12301 gcccaaaaat tagtggctgc aagcggtcta aaacgggcat ttttttgcaa ttcaggggca
    12361 gaggcaaatg aaggagcaat taaaaccgcc cgcaaatata gtttcgacaa atacggttcc
    12421 ggtcgttcaa cggttttaac cctcgtgaac tcttttcacg gacgaaccat ttcaaccctc
    12481 gcatccactg ggcaagaggt attccacaag cattttttcc cctttacaga gggctttgaa
    12541 tatttacctg taaatggcat tgaggcactg aaagctcgct tggaaactga gaacgtttgc
    12601 gggattttgc tggaggtggt gcagggcgag ggcggtgttt gcccattaga ggcggattat
    12661 ctgcacgccg tgcaaagcct gtgccaagaa aaagatattt tatttatgat tgatgaggtg
    12721 caaaccggca ttgggcgaac gggcacactt tttgcttatc aacaatttga tttaactcct
    12781 ggtttagtca cgctcgctaa aggcttgggc ggcggcttgc cgattggtgc tttcctgtta
    12841 ggcgaaaaag tggaaatgac attaggcaaa agcgaccacg gctcaacctt tggtggcaat
    12901 cctgtttcct gtgctgctgc aaatgtagta atcgataaaa taaacgccga atttctcgcc
    12961 caagtgcaac aaaaagggca gttgttacag caacgcttag cgagcttacc aaaagttaaa
    13021 tccgtttcag gcttaggttt aatgctcggc gtagaatttg aggaaggcat tttagccgct
    13081 gatattgttg caaaatgtat caacaaaggt gtgctgtttt taaccgctaa aagcaagtta
    13141 aggctactcc caccgctgat gatctctgaa caacagatag aacaaggaat cagagtgtta
    13201 gaggatgtct tgaaagattc ttgagttatt ataa
//
LOCUS       DANLXS010000061        12905 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.061, whole genome shotgun sequence.
ACCESSION   DANLXS010000061 DANLXS010000000
VERSION     DANLXS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.061"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..579)
                     /locus_tag="PXD71_001258"
     CDS             complement(<1..579)
                     /locus_tag="PXD71_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HDL6291387.1"
                     /translation="MMSFLSEVFSSFKPTKTEVISSEPNENSGESDIIQNKMSEVNNE
                     VQQLKRSGKLTQSQHHRRLYSQEPSFIDHLPWGEYLNEHGVILLDDARSVGAVFDIKP
                     IGTAGRSIEFLSRIRNLVKDALQDSFDELDVNPYVVQFYCQDSDDLQPYIEQLRNYIS
                     PIAKNSAFSEEWLRVQAKHFKDIGKPEGLFIDE"
     gene            complement(579..977)
                     /locus_tag="PXD71_001259"
     CDS             complement(579..977)
                     /locus_tag="PXD71_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL6291388.1"
                     /translation="MKALYCIMMSALLITGCTTSTEELLPANGSTMRDIWDKGAGNND
                     LHLYRSQQGRTLDPVDYIPNTEQRAYTRTAENEINNLFPRLPNPDLIMYVYPHQTRSG
                     EPLSIPGYSTVLPFYGRVQYAQPGDRTRGL"
     gene            complement(990..1562)
                     /locus_tag="PXD71_001260"
                     /pseudo
     CDS             complement(990..1562)
                     /locus_tag="PXD71_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
     gene            complement(1809..1994)
                     /locus_tag="PXD71_001261"
     CDS             complement(1809..1994)
                     /locus_tag="PXD71_001261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291389.1"
                     /translation="MNEQNNSWVQEWLNKSAKASERMATDEKFRQEVAKRATNSGQDI
                     QAILAKNIAKYQNQAKD"
     gene            2066..2215
                     /locus_tag="PXD71_001262"
                     /pseudo
     CDS             2066..2215
                     /locus_tag="PXD71_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
     gene            2216..2809
                     /locus_tag="PXD71_001263"
                     /pseudo
     CDS             2216..2809
                     /locus_tag="PXD71_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
     gene            complement(2963..3778)
                     /locus_tag="PXD71_001264"
     CDS             complement(2963..3778)
                     /locus_tag="PXD71_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDL6291390.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(4114..4950)
                     /locus_tag="PXD71_001265"
     CDS             complement(4114..4950)
                     /locus_tag="PXD71_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDL6291391.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(4950..5753)
                     /locus_tag="PXD71_001266"
                     /note="aph(3'')-Ib"
     CDS             complement(4950..5753)
                     /locus_tag="PXD71_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDL6291392.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(5922..6767)
                     /gene="sul2"
                     /locus_tag="PXD71_001267"
     CDS             complement(5922..6767)
                     /gene="sul2"
                     /locus_tag="PXD71_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDL6291393.1"
                     /translation="MEYTSKSQIARNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQA
                     RKLMAEGADVIDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQ
                     AYALSRGVAYLNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHI
                     AAFFDARIAALTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLL
                     SVSRKSFLRALTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKE
                     TARIR"
     gene            6930..8222
                     /locus_tag="PXD71_001268"
     CDS             6930..8222
                     /locus_tag="PXD71_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL6291394.1"
                     /translation="MGTYIPHREGIIQTIVRNSLTQYQAYASPSQTILDRLEHFAECA
                     DWTKGVVRIRCHDCGHSYFRPFSCKVFHLCPSCDQKRTLLYAEYLAEDLLLDLPHRQF
                     VFTIPKILRPYFKSDKRLFGEVSRLVFSLLSDFFSLAAGQELLCACVVSYQSFGEFAR
                     FHPHWHVLVLEGGFTKYDRFVYLPIGADEGMLKVWQAAIMALFLRKELIDQARVNMLR
                     DWKHSGFSIESETRLFNKADREALGQYVVRGATCAEKIQYDPASDTIIWTASPKGFYK
                     GKTETFKGFEFVDQLVAHLPPRRVQLLRRYGVYAGKVRKQWQERPNIYSLAPESWQKG
                     HPRESKIVPAIPQEKPETVQVSDAWSKLRKQSWARLLQKVYEVDPFVCPKCQGTMSVV
                     AIIEDPKELAKIINWAKQQEREQPVAVCARSPPELALV"
     gene            8562..8819
                     /locus_tag="PXD71_001269"
                     /pseudo
     CDS             8562..8819
                     /locus_tag="PXD71_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            9288..9734
                     /locus_tag="PXD71_001270"
                     /pseudo
     CDS             9288..9734
                     /locus_tag="PXD71_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
     gene            10011..11225
                     /gene="floR"
                     /locus_tag="PXD71_001271"
     CDS             10011..11225
                     /gene="floR"
                     /locus_tag="PXD71_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HDL6291395.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSVLAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNSDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            11253..11558
                     /locus_tag="PXD71_001272"
     CDS             11253..11558
                     /locus_tag="PXD71_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6291396.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
     gene            11670..>12905
                     /locus_tag="PXD71_001273"
     CDS             11670..>12905
                     /locus_tag="PXD71_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HDL6291397.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRV"
BASE COUNT         2968 a         3420 c         3436 g         3081 t
ORIGIN      
        1 ctcatcaata aaaagcccct ctggtttacc gatatccttg aaatgcttgg cttgtacccg
       61 taaccattct tcgctaaatg cactattttt agcgataggc gagatataat ttcgtagctg
      121 ttcaatataa ggctgtaaat catcgctgtc ttggcaataa aattgaacaa cgtacggatt
      181 tacatccaat tcatcaaagc tgtcttgcaa ggcatctttg acaagattac gtatccgact
      241 taaaaattca atactacggc cagcagtacc aatcggctta atatcaaata ctgccccaac
      301 actacgggca tcatcaagga gtatcacacc gtgctcattc aaatattctc cccaaggtag
      361 atgatcgata aaggagggct cttgtgagta taaccgacgg tgatgctgtg actgggtgag
      421 ttttccactg cgttttaatt gttgaacctc gttatttacc tcactcattt tattttgaat
      481 gatgtcagac tcacctgaat tttcatttgg ttcacttgat ataacctctg tcttagtcgg
      541 tttgaaagag gaaaatacct cacttaaaaa agacatcatc aaagccctct tgttcgatca
      601 cccggttgag catattggac tcgcccgtag aaaggtaata ccgtagaata tcctggaata
      661 ctaagaggct ctccggaacg agtttggtgt ggatacacat acataatcag atccggatta
      721 ggtaaacgag ggaacaagtt attaatttca ttttctgccg tacgggtata ggctcgttgc
      781 tcagtgttag gaatataatc gacaggatcg agtgttcgtc cttgttgtga acggtataag
      841 tgtagatcgt tattacctgc tcctttatcc caaatatctc gcatcgtact gccgttagcg
      901 ggtaataatt cttcagttga agttgtgcaa cctgttatca ataaggcact catcattatg
      961 caatataaag ctttcattgt ttgttccttt taatcaagtt gtgtattagt tgatgtgttt
     1021 ccgtatttca ctttacgtcc tttggagttg taatcgatct caatctcttt ctcgatatga
     1081 atagcgacac gatgtccagg tggtacatag acagcatcaa agttttcagc aaaacgcttt
     1141 ctaaaccagt cggcggattc ttttaaacca ccgccgatgg cttgccctaa gatgaactta
     1201 ccttgatcac cggtaacagc tccaacaaca ctattgccat caactactgt cgttgtttgt
     1261 gattgtgaaa acccttgggc tgcggcactg gcagccgcta agaagaaatt tgtgcctaaa
     1321 tattctggtg catttgtttt tctctccccg ggaacacaag gaatgcctga actatcagat
     1381 aaccaaccga tattctcact attccctttg ttagtcgtgc tgacaatacg cccatcattg
     1441 aacacaaagg tcatactttt tacatcgcct cgtacacaag atagagtcca gtcgccagta
     1501 gctgtacccg aaacaatcgc accttctaca tcaagtaatt caataccatt ggcaatgaga
     1561 tggtttagcc ttagttctca atgcttttag ctgattttct aggatatttt gggcgatttg
     1621 ctccaatcgg tcgttaaatt ctcgcaaaaa ctccatagtc tgccgatact gttctgatga
     1681 caatagatta gaatttgcgt tattttgcgt tttaggcgtg tttgttggtg tgggtgctac
     1741 cttactacca tttttatttt tgataggtct tgtaggctct atgagagcgt tttcagacgt
     1801 tttattagtt aatctttcgc ctgattttga tatttggcga tattcttggc tagaattgcc
     1861 tgaatatcct gtccgctgtt cgttgctcgt ttggcgactt cctgacgaaa tttctcgtcc
     1921 gttgccattc tctcgctcgc tttcgctgac ttgttcaacc actcttgaac ccaagagttg
     1981 ttttgctcgt tcatctgatt tttcctgtgt ctgtgattgt gtggatttta aggtttggta
     2041 agccaaaact tgctcgttat acgccaagga ggacacccgg cgcaaggtgc tggtaggcgc
     2101 gtggatactc ggcaaggttg aaagcggcga gtggccagaa cagcgcctgc tcgatgggtt
     2161 ggatagctac ctagagcgcg accatgatcg ggcgctgttc ggactgccac ccaaggggtc
     2221 gatttgcggc cacacctttg cggtaaatga aaaaggtatc gttaacacgg tgaataacat
     2281 ccgtgcagtg tatcgtccgc agggattacc gcgtcagatc ctggctcgcg cctcgctgaa
     2341 tgcgaacacc ctcgacgagg ccatcactat acttaccggc cagccgcgat ccggggcgtt
     2401 tcaccatacg ctcgggcagc tgggcgatgc gcggctgttc agcgttgagg ccaccggtca
     2461 aggctgttcc gttctacccc tgacgacggt aaccgggcat gccaaccatc tggtgcatgc
     2521 ggcactggct ggcgttgagc agatcgttac cgacagttca gcgtcgcggc agttacgcct
     2581 cgtgcagtgg cgggaaacgc aacctccgtt tgacgccgcg gcggcaaaaa ccattctctc
     2641 agatacccat gacgcagaac tgccaattta ccggctggct gccgacgatc cggatgagga
     2701 gaacaccctc gccacggcag ttttcaccct cgatgccaac cacgtcaggt ggcaaatttt
     2761 cgacattaac cgcgacgatg ctaaatttca tggagaagtg cgtgggtgaa atagccggtc
     2821 gtcagtcata aagggcaggg gagtaccgtt ggcccggcgt tcgatagtac cgtccggata
     2881 ctgccagata tcggcgtcca gggagatgtc ctgaagcgcg gtgtccggaa tttcaggttt
     2941 gtgtctctac aaagactaac tatcagaaaa actcatcgag catcaaatga aactgcaatt
     3001 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     3061 aaaattcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     3121 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     3181 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt
     3241 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     3301 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
     3361 gacaattact aacaggaatc gaatgcaacc ggcgcaggaa cgctgccagc gcatcaacaa
     3421 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggagcg
     3481 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     3541 gcataaatgc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     3601 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     3661 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     3721 ccaggttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa
     3781 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     3841 tatcttgtgc aatataacat aagagatttt gagacacaac gtggatttgt tgaataaatc
     3901 gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg
     3961 tggcaaagca aaagttcaaa atcaccaact ggcccaccta caataaaggg gtcgtttgcg
     4021 ggagagggcg aaatcctacg ctaaggcttt ggccaacgat attctccggt aagattgatg
     4081 tgttcccagg ggataggata agtcgcttga tatctagtat gacgtctgtc gcacctgctt
     4141 gatcgcggcc gcgatagcta gatcgcgttg ctcctcttct ccatccgcgt tccaagctgc
     4201 ggaaaggcac ccataagcgt acgcctggtc gagcaggcga cgcggatcga cgtccagcgc
     4261 acgagagaat gcgtccgcca tctgtgcaat gcgtctagga tcgagacaaa ggtcgtctct
     4321 gtcagccgga tcgtagaaca tattggcggc gccaaagccc acttcaccga ccagaccgac
     4381 gggatctatc accagccagc cgcgactgga gaacatgatg ttttcatgat gcagatcgcc
     4441 atgtagccca cgcagttccg aggcattgct catcatttga tcggctataa tcgccgcgtg
     4501 gacgtagtca gtttgacaac ctgcgttttg atcatcgcgc gcccgctgaa acaaagctgc
     4561 aaagcgatcc cggatcggga gaagggcaga aggcaggggt tcctcagatg cggcatacag
     4621 cttcgccatc agttccgctg caatttcggt cgcctggtag tcgccgtgct cggcaacgat
     4681 gtgagagagc attcgctccc cggcatattc gagcaacatc agattgttct cacgaccgag
     4741 caaccggact gctcccctcc cattgcgcca taccagatag tcggccccgc gcagttcatc
     4801 agcaatgtct tctataggtt tcaatccctt gacgattgca ggagtcccgt ctggcaatga
     4861 aactttccaa acgaggctgg aaaaggtgtc cgcaatgaga acaggttgcg aaacgtgcca
     4921 atgagcagga aaaacaggcg gcatgaacat caaccccaag tcagagggtc caatcgcaga
     4981 tagaaggcaa ggcgttcgcg gtcgggggct tcgatcccca atacattgaa taggacagcg
     5041 aaggcgcgct ctgcttcatc tggcgctgcc cagttctctt cggcgttagc aatcatgagt
     5101 gccaaatcgg catagcgatc tgctgttccg agccgcccaa ggtcgatcag acccgtgcat
     5161 tgaagagttt tagggtccac catgaagttc ggcatgcagg gatcaccatg gcaaacaacc
     5221 atatcggtgc gctcttggtc gagccgcacc ggtagctctc gttcgacacg agccaaaaga
     5281 tcgagctgcg gcgtactctt gtcctcgtcc ggtaagaagt cgggattgac ggcattgcgg
     5341 gacaccacat caacggcgcg tccgaacatt cgcgacagcc tgcgctcaaa cggacattga
     5401 tcaaccgata ggctgtgaac agcgccaagt tgctgcccca ttgacggcca cgctttgagc
     5461 aaatccgctc cagacagatc agccgccggt actcccggaa ttgccgttat caccaagcat
     5521 gcaccctcct gttcctcctg ccagttgatc acctcggggc aagccacacc tcgacctttg
     5581 agccaaatga ggcggtcacg ctctccagcg agctcaccgc ggcgggaagc aggtgcgatt
     5641 ttcgcgaagg catgcccgtc accacgtcga aaaacaaaat caccggattc tccgcctctg
     5701 acaggcaacc agtcagaatg cgattcacca aaaaaaatat tagttcgatt caatggaggt
     5761 tccttcagtt ttctgatgaa gcgcggaggt ggctttgcca cgaataccat ctgtgccgaa
     5821 atatttgcgc accataaggt cgattatcct gtcgtcgggt cgccctcaaa ggggacatgc
     5881 ctgctgaacc gcgaatatag agaaatatcc cgaatgtgca gttaacgaat tcttgcggtt
     5941 tctttcagcg ccgccaatac cgccagcccg tcgcgcaagg ggcgcggctc gtgtgtgcgg
     6001 atgaagtcag ctccacctgc ggcggcggca agctctgcag cgagtgtcgc ggccccgaca
     6061 tcccccggac cacggcctgt gagcgcgcgc agaaaggatt tgcgcgaaac agacagaagc
     6121 accggcaaat cgaagcgcag ccgcaattca tcgaaccgcg ccagcaccga gagcgaggtt
     6181 tcgggagcag cccccagaaa aaaccccatg ccgggatcaa ggacaaggcg gttgcgtttg
     6241 ataccggcac ccgtcagcgc cgcgatgcgc gcgtcaaaga acgccgcaat gtgatccatg
     6301 atgtcgccag cgggtgcctc gcgccgatct gcctgcccgt cttgcaccga atgcataacg
     6361 acgagtttgg cagatgattt cgccaattgc ggatagaacg cagcgtctgg aaaaccgcgg
     6421 atatcattga gataggccac accacgcgac aaggcatagg cttgcgtcgc gggttgataa
     6481 ctgtcgagcg agacgggaat gccatctgcc ttgagcgcgt ccagcaccgg cgcgatacgc
     6541 gcgatttctg tgtcggacga aacaggcgcg gcgtcgggat tgctggatgc cggaccgagg
     6601 tcgatcacat ctgccccctc ggccatcagc ttacgcgcct gcgcaatggc tgcgtctggc
     6661 gccagatacc ggcctccatc ggagaaactg tccgaggtta tgttgacgat gccgaaaatg
     6721 atgagcgatt tatttcgagc tatttgtgat ttacttgtat attccatttt cctccgcgcc
     6781 gaaggcgtca acctaacttt cggttgagct gcgaacgggc ttgtcccgtg agtccgctcg
     6841 aaccggttgt taggtggcgc cagtccacat tttccattat tttcttcgca attgcaagca
     6901 aatgatcgtt ttcctgcgta tgtataggta tgggaactta cattccccat cgggaaggga
     6961 taatccagac catagtccgc aattctctaa cccagtacca agcctacgca agtccatcgc
     7021 aaactatcct tgaccgcctt gagcattttg ctgaatgtgc cgattggacc aagggtgttg
     7081 ttcgaatacg ttgccatgat tgtggacatt cgtacttcag gccattttcg tgcaaggttt
     7141 tccacctttg cccatcctgt gaccaaaaac ggaccttact ctatgctgaa tacctggccg
     7201 aggatttgct ccttgatcta ccgcatcggc aatttgtttt tacgattccc aagattctca
     7261 gaccatactt taaatcggat aagcgactgt ttggcgaggt gtccaggctg gtattctcct
     7321 tgctttctga ttttttctcc ctggcagccg gtcaagaact actatgtgcc tgtgttgtca
     7381 gctatcagtc gtttggtgaa ttcgctcgat tccacccgca ctggcatgtt ctggttctgg
     7441 aaggtgggtt caccaagtac gaccgctttg tatatttacc aattggagcc gacgaaggga
     7501 tgctcaaggt ctggcaagca gcgattatgg ctttgtttct gcgaaaggaa ctaatagatc
     7561 aagcccgagt gaacatgctc agagactgga agcattccgg ctttagcatc gagagcgaaa
     7621 ccagactatt caacaaggcc gatcgagaag ccctcggcca gtatgtcgtt cgcggcgcta
     7681 cctgtgcaga aaaaattcag tatgatccag cttccgacac gataatctgg acagctagcc
     7741 caaagggatt ctacaaagga aaaacagaga ccttcaaagg ctttgaattc gtcgaccagc
     7801 ttgtggccca tttaccacca aggcgggttc aattgcttcg tcgctatggc gtatatgcgg
     7861 gcaaagtccg caagcagtgg caagagcgcc ccaatattta tagcctggcc ccagaaagtt
     7921 ggcaaaaagg tcatccacgc gaatccaaaa ttgttccagc catacctcag gagaaaccgg
     7981 aaactgtcca agtttccgat gcctggtcca agttgcgcaa acaaagttgg gcacggttgt
     8041 tacagaaagt ctatgaagtt gacccgtttg tttgcccaaa atgccaggga acaatgtcgg
     8101 ttgtggcgat aatcgaggat cccaaggagc ttgccaagat tattaactgg gcaaagcagc
     8161 aggaacggga gcagccagtg gccgtctgtg ctcgttcacc tcctgagctt gctttggtgt
     8221 aggtataata cccaaaaacg aagtttatgc ggaacaggat aacacctcga aatcggagtt
     8281 tatatggctt cgtgggggag ttatgtgttt gagctttgtc gcattaacgc gaatcagggg
     8341 ttgacggcag aaatagactg gaattacact tcttggagtc ggcgttgccg gaaaattctg
     8401 attggattag ttgttcgggg tgcgaaaaca gtcgtagttc ggggaaaaaa ccgaattttg
     8461 acccgaaacc gcaaaaaacc cgaaatgaca gttcctataa tttatttatt tgggggcgtg
     8521 cacctgtagc cttagcgact ttaaatgaca gttcctataa ttctgatgaa ccggcaacac
     8581 cagcacaacg gcgagcgtcg atgacatggg cgcaacggct caagcgtgtt ttcaatatcg
     8641 acatcgagac ctgcagcggc tgcggcggcg ccatgaaagt catcgcctgc attgaagacc
     8701 ctatagtgat caagcagatc cttgatcacc tgaagcacaa agccgaaacc agcgggacca
     8761 gggcgttacc cgaaagccgg gcgccaccgg ctgagctgct cctgggtctg tttgactgac
     8821 gagcctgaag gccaacgata ccaatcaaaa tgctgcgttc acagcgccgc ggcagggatc
     8881 cgccgtgctg gttgtcggaa aaggagccgc tagtgggaaa gaggagggta aattttcagc
     8941 gttgctggct ccccgtcagc cggattgggt tgcatcgcag gggtgtcgaa agagtcaact
     9001 gcggtccaaa gctgttggac ttgggtgaaa agggcgttta ttcttcctat acgtgccgcg
     9061 ttccaggcgg cgactatgag ggctatgtcg atgcccatgt gcgccggctg gaggcgctac
     9121 gccgggccgg tatcgtcgag cggatcgacg ccgaccaatg gcgcatcccc gatgatctgg
     9181 tcagccgtgc cgccgcccat gacgccggcc gagacagtca ggccagcgtt cgcgtccttt
     9241 ccccggtcga tctgaacaaa cagatcggat cggacggcgc gacctggctg gaccggcggc
     9301 tgatccacgg cgagacggcc gaccttgcgc caaccggctt cgggcaacaa gtccgcgaag
     9361 ccatggacca gcgccgcgag caccatatcg aacagggcga cgccacccgc agccgggaca
     9421 gccgcgtctt ctaccggcgc aaccttctcg ccatcctgcg ggagcgcgag gtagccggcg
     9481 tcggatcgga tatggctttg agtaagggcc tgccgttccg cgccgccacg gacggcgaga
     9541 gcgtcagcgg caagtttacc ggaaccgtgc atctatcgag cggcaagttc gccgtggtcg
     9601 agaaatccca tgagttcacc cttgtcccgt ggcggccgat catcgaccgc caactcggcc
     9661 gcgaggttat gggcatcgtg cagggcgggt cggtgtcgtg gcagttaggg cggcagaggg
     9721 ggctggaacg ctgagtgcgc ccatgccgca ttgcgaagca aaagataatc ggataaaatg
     9781 tagcaattca tattcgtaag cgtggagtaa tcagatggga aattccaagt cagcagacaa
     9841 gtaagccgca acaaccagta ttgttgttgc ggcgctctgt aaggctagtc tcatctgatt
     9901 gctgacgagc agacgtcgcc cggtattcct taatcgaggg ttgattcgtc atgaccacca
     9961 cacgccccgc gtgggcctaa acaaatatca atcgcaagga gatcatattc atgaccacca
    10021 cacgccccgc gtgggcctat acgctgccgg cagcactgct gctgatggct cctttcgaca
    10081 tcctcgcttc actggcgatg gatatttatc tccctgtcgt tccagcgatg cccggcatcc
    10141 tgaacacgac gcccgctatg atccaactca cgttgagcct ctatatggtg atgctcggcg
    10201 tgggccaggt gatttttggt ccgctctcag acagaatcgg gcgacggcca attctacttg
    10261 cgggcgcaac ggctttcgtc attgcgtctc tgggagcagc ttggtcttca actgcaccgg
    10321 cctttgtcgc tttccgtcta cttcaagcag tgggcgcgtc ggccatgctg gtggcgacgt
    10381 tcgcgacggt tcgcgacgtt tatgccaacc gtcctgaggg tgtcgtcatc tacggccttt
    10441 tcagttcggt gctggcgttc gtgcctgcgc tcggccctat cgccggagca ttgatcggcg
    10501 agttcttggg atggcaggcg atattcatta ctttggctat actggcgatg ctcgcactcc
    10561 taaatgcggg tttcaggtgg cacgaaaccc gccctctgga tcaagtcaag acgcgccgat
    10621 ctgtcttgcc gatcttcgcg agtccggctt tttgggttta cactgtcggc tttagcgccg
    10681 gtatgggcac cttcttcgtc ttcttctcga cggctccccg tgtgctcata ggccaagcgg
    10741 aatattccga gatcggattc agctttgcct tcgccactgt cgcgcttgta atgatcgtga
    10801 caacccgttt cgcgaagtcc tttgtcgcca gatggggcat cgcaggatgc gtggcgcgtg
    10861 ggatggcgtt gcttgtttgc ggagcggtcc tgttggggat cggcgaactt tacggctcgc
    10921 cgtcattcct caccttcatc ctaccgatgt gggttgtcgc ggtcggtatt gtcttcacgg
    10981 tgtccgttac cgcgaacggc gctttggcag agttcgacga catcgcggga tcagcggtcg
    11041 cgttctactt ctgcgttcaa agcctgatag tcagcattgt cgggacattg gcggtggcac
    11101 ttttaaacag tgacacagcg tggcccgtga tctgttacgc cacggcgatg gcggtactgg
    11161 tttcgttggg gctggtgctc cttcggctcc gtggggctgc caccgagaag tcgccagtcg
    11221 tctaaccgac gactggtagc aggcccgctc cgatgcggcg cactaaccat cgaaacctcg
    11281 tgaatgtcgg tatcctgtct ggcaggatac cgctcatttc ccttgttcag ttcatcgccg
    11341 tcgccgagca tctgaatttt cggcatgcgg ccaaggcact tggtatcagc cagtcgagcg
    11401 tcagcgcgcg tgtgaaagcg ctggaggata accttggtgt cctgctattt gagcgccatg
    11461 cgcggggcgt tcggctaaca gacgcaggca ggcacttcat ggagcgtgtc acggcgggtg
    11521 tcgatcaact cgatcacgca gtgaagaccg cggagtgacg ggcactggct ggcaatgtct
    11581 agcaacggca ggcatttcgg ctgagggtaa aagaactttc cgctaagcga tagactgtat
    11641 gtaaacacag tattgcaagg acgcggaaca tgcctcatgt ggcggccagg acggccagcc
    11701 gggatcggga tactggtcgt taccagagcc accgacccga gcaaaccctt ctctatcaga
    11761 tcgttgacga gtattacccg gcattcgctg cgcttatggc agagcaggga aaggaattgc
    11821 cgggctatgt gcaacgggaa tttgaagaat ttctccaatg cgggcggctg gagcatggct
    11881 ttctacgggt tcgctgcgag tcttgccacg ccgagcacct ggtcgctttc agctgtaagc
    11941 gtcgcggttt ctgcccgagc tgtggggcgc ggcggatggc cgaaagtgcc gccttgctgg
    12001 ttgatgaagt actgcctgaa caacccatgc gtcagtgggt gttgagcttc ccgtttcagc
    12061 tgcgtttcct gtttgccagc cggcccgaga tcatggggtg ggtgctgggc atcgtttacc
    12121 gcgtcattgc cacgcacctg gtcaagaaag cgggccatac ccaccaagtg gccaagacgg
    12181 gcgcggtcac cctgatccag cgttttggat cggcgctcaa tctgaatgtt cacttccaca
    12241 tgctgtttct cgacggtgtg tatgtcgagc aatcccacgg ctcagcgcgt ttccgctggg
    12301 tcaaggcgcc gaccagccca gagctcaccc agctgacgca caccatcgcc caccgggtgg
    12361 gtcgctatct ggaacggcaa ggcctgctgg aacgggatgt cgaaaacagc tatctggcct
    12421 cggatgcggt ggatgacgac ccgatgacac ccctgctggg gcactcgatc acttaccgta
    12481 tcgctgtcgg ttcacaggcg gggcgaaagg tgttcacttt gcaaactctg ccgaccagtg
    12541 gtgatccgtt cggtgacggg attggcaagg tagccgggtc cagcctgcac gccggcgtgg
    12601 cggccagggc cgatgaacgc aagaagctcg aacggctgtg ccggtacatc agccgcccgg
    12661 cggtatccga gaagcggctg tcgttaacac gaggcggcaa cgtgcgctac cagctcaaga
    12721 cgccgtaccg ggacggcacc acgcacgtca ttttcgaacc attggatttc attgcaaggc
    12781 tggccgccct ggtaccgaag cccagagtca acctaacccg cttccacggg gtgttcgcac
    12841 ccaacagtcg gcaccgggcg ttggtcacgc cggcaaaacg gggcaggggc aacaaggtca
    12901 gggtg
//
LOCUS       DANLXS010000062        12809 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.062, whole genome shotgun sequence.
ACCESSION   DANLXS010000062 DANLXS010000000
VERSION     DANLXS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.062"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..925
                     /locus_tag="PXD71_001274"
     CDS             <1..925
                     /locus_tag="PXD71_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDL6291398.1"
                     /translation="QLLTAKPINKEVLNQVLKTVFDNEHLLGIDLQVYGAEYWNVSID
                     NKPSLDWVSAEIAIENEKLGILSWKPSLVCPGERLIQNVSEMIGRSLYVVQMQKQQQQ
                     LVLMEERAIIARELHDSLAQSLTFFKIQVSLLKLAKTDEKRNEILTVFEKALNDAYSQ
                     LRELLTTFRLTIQEANLQQALEKILESLRPKTSAQIRLSCKLPSHMFNAQQQVHVLQI
                     VREAVINAIKHSQATEINVIAETNDDGEHCLTIQDNGVGLAAHTQPEGHYGLTIMQER
                     AAELKAEFSIENVESGGVKVQVLLPSMISKR"
     gene            976..1494
                     /locus_tag="PXD71_001275"
     CDS             976..1494
                     /locus_tag="PXD71_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL6291399.1"
                     /translation="MQFRKAVEADFETILAIYNQAIPTHQITADLELATTQNRRKWFD
                     FHLETAQYPIWVVEDEKGIAGWFSFSPFYERPAFLHTSEISIYLDRYSKGKGYGSKII
                     EFMQAEMLNHNIHTLMAYVFELNTVSQNLMRKHGFEQWGRFPNIANMGKDEKGEDKWR
                     TLLMMSYQKGIE"
     gene            1537..2136
                     /gene="rdgB"
                     /locus_tag="PXD71_001276"
     CDS             1537..2136
                     /gene="rdgB"
                     /locus_tag="PXD71_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDL6291400.1"
                     /translation="MEKTKIVLATSNADKVKEMADVLSQFGFEVVAQSEFGIESPEET
                     GLTFVENALIKARFAAKMTGLPAIADDSGLSVMALGGEPGLYSARYAGEQATDADNRQ
                     KLLAKMQGQENRLAKFVSCIVFLKHETDPTPYIASGECFGEILTEERGENGFGYDSLF
                     FYPPKACTFAELETKEKKAISHRAIALEQLKQQLAGENK"
     gene            2133..2453
                     /locus_tag="PXD71_001277"
     CDS             2133..2453
                     /locus_tag="PXD71_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HDL6291401.1"
                     /translation="MIITTTPTIEGKQIVEYKGLVFGEVVSGANFIRDFLAGITDIIG
                     GRSGAYESKLNGARKEALAELAKEAQKVGANAVVGVSMEYQSMGGDKGMFIVVATGTA
                     VVIR"
     gene            2457..2909
                     /locus_tag="PXD71_001278"
     CDS             2457..2909
                     /locus_tag="PXD71_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDL6291402.1"
                     /translation="MQFEQHFEANFWQNKSLLEMNDAEWEALCDGCGKCCYRKYIQGR
                     GRRERLYYTSVACNLLDVNTGRCMNYSNRFKIESDCTKLTKKNLPDFGWLPKTCAYRL
                     LYEGKPLFDWHPLISKDPDSVKNAGMLIANGIHEKDVIDWFEFVIDEV"
     gene            complement(2950..3531)
                     /gene="lpcA"
                     /locus_tag="PXD71_001279"
     CDS             complement(2950..3531)
                     /gene="lpcA"
                     /locus_tag="PXD71_001279"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDL6291403.1"
                     /translation="MYLEQIKAELQEAADVLDKFMSDEKNIKLIQDAAMLIANSFKQG
                     GKVISCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHLSCVSNDFGYEYVFS
                     RYLEAVGQKGDVLFGLSTSGNSKNVLNAIKVAKEKGMKVIAMTGKDGGQMAGLADVEI
                     RVPHFRYADRIQEIHIKVIHILMMLIEFEMEKA"
     gene            3840..4799
                     /locus_tag="PXD71_001280"
     CDS             3840..4799
                     /locus_tag="PXD71_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL6291404.1"
                     /translation="MVVNPESFEQIIGQSVKIKEVVEQAKKFANLDAPLLIQGETGTG
                     KDLFAKSCHHFGSRRMQKFIAVNCAGLPAEEAESEMFGHRGNGVESVGFFEYAHGGTV
                     LLDSISELSLEMQAKLLRFLNDGSFRRVGEDQEIQVNVRVICTSLQPLSLLVEKGKVR
                     EDLFHRLNVLTLNLPPLRDRKEDIPLLADYFIAQISNQLGVSKLEYDNVFLQALQNYH
                     WRGNLRELYNALYRACTLSHSYRLAVKDLHLPRDMEAMIEVREEATLEEMMSQYEAQL
                     LHQFYAEYPSTRKLAQRLGLSHTAVANKLRQYGLTKNGNSKDE"
     gene            4792..6543
                     /locus_tag="PXD71_001281"
     CDS             4792..6543
                     /locus_tag="PXD71_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6291405.1"
                     /translation="MNKLAVIFSVCFANLGLNSTAYAAPSIPQELLENSLVYCTTSSG
                     FSFNPQKSDVSSNMNVVTEQIYDKLVEFNPKKNRLEPALAESYSISDDGLVITLRLRK
                     NVAFHTTPWFTPSRKLNAEDVVFSLKRMIGKESDLPEVKSEPESVLHHQRQSYIYQKT
                     AGQIYFPYFESVDLANKIASIHSPSNDIVKIELVRPDSALLAHLASQYAVILSKEYAL
                     QLNADDNLVQLDLLPVGTGVYQLESYENNDYVRLKPNMNYWGKKAYIQNMVVDFSTEA
                     TGRMAKFLNNECDVVAFPEPSQLSVLNNRQLISSKGANLVYLAFNMQRGIGQDLALRQ
                     RIARGINRKRIAEKLFYGTGEVADNLLPTALWSKKNNGSYPYQPHQNSGEQAVEMHDS
                     IINRSLDSKTETEEVNQKQRLKLWVIDEKRVYNQHPLKMAEMIRADLATQNLEVEIRA
                     VSRAYLVQQLANKSADYDLILGGWLANNLDPNSFLEALLSCKAAETVTNLSNWCNEDF
                     DFWLQTARLSDDPASANLLYEFAQKLLEEQLPILPLMNANRVLVIKDGVKQADISPFG
                     QVKLSELRLGLGHKKGN"
     gene            6546..7511
                     /locus_tag="PXD71_001282"
     CDS             6546..7511
                     /locus_tag="PXD71_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6291406.1"
                     /translation="MLIVFFRKIFLTLITLIFLSLISFNILLRDPLNHLAELGWFEAY
                     CYYVKSLLNGDFGISFSNGEPLTAQILQVFPATITLCLSALIVSLIIGLPLGFFAATQ
                     QKNMVGRLLNTLGSLSLAVPVFWLALVLMYYASLNQWEVSAIGEIHSLYPKQMVTGFL
                     LLDIWLSDVPYKLKMMQSALHHLALPTLVLAVPATLEVMHITQARAGYVMKQNYIKIA
                     KTRGWTPFRVWRTHIICNTLPALLPMIARTFILIFAFGMLIENIFSWGGIGRWLINAL
                     AIQDYNAISAGVVAIGVFVLLVDMVTGFVRVMLDPSDKKDWYGSY"
     gene            7498..8391
                     /locus_tag="PXD71_001283"
     CDS             7498..8391
                     /locus_tag="PXD71_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6291407.1"
                     /translation="MAVIKEPEQFRETNAFKAFWFHFRQDKLALGSFYLFLLLLALIF
                     VGSLLAPYNYNEQFVGLELMPPSWDDKGQISHFLGTDDLGRDILSRIMYGFYYTLGSA
                     LMITLLIAIIGGMIGIYAGLKKGRSFWLISHLFDTFLFMPILLIAIIIATLMEASLVN
                     AMLAIFLAMLPHFIHKIYQATEKQLKKEYIITLKLDGASCWYLIREVIIPNLTGVAIK
                     ALSHIFVIAILDISALSFIMLGAKSPTPEWGAMIRESMELIYIAPWTVILPGLAIIFS
                     IFTVTILGTRLSVIFEKYRKE"
     gene            8462..9514
                     /locus_tag="PXD71_001284"
     CDS             8462..9514
                     /locus_tag="PXD71_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL6291408.1"
                     /translation="MALLDIRHLSIEIDMPKGRVKMVDNINLTVDEGEICGLVGESGS
                     GKSLIAKVICGMIKDEWIVSADRFRFDDIELLKLSPAERRKLVGDHISMVFQDPLTSL
                     DPSQQIGKQLMQTIQFRGKWWQRFGWKKKKAIELLHRVGIRDHQDIMKSYPSDITEGE
                     AQKVMIAMAVANQPRLLVADEPTNIMESTTQLQVYRLLSSMNQNLGTSILLVSNDMRS
                     IQNWVDSYNVLYCGQTVEIADKESILESSYHPYTRVLLNSSPDFSQPLAFKSSLNTLR
                     GSVPMLQEMPIGCRLGPRCPFAQKKCINKPPLVKVKQHEFACHFPINFREQNLLKNKT
                     ENTPFVVAEIAEKEAF"
     gene            complement(9523..9753)
                     /locus_tag="PXD71_001285"
     CDS             complement(9523..9753)
                     /locus_tag="PXD71_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDL6291409.1"
                     /translation="MKLTACKLVFLLYLMFNLTACGTVLSLVEQDYRVYAGVIKDFQV
                     MQEGGIFAVLAVIDLPLSFVLDTLMLPVTLSQ"
     gene            9883..10965
                     /gene="leuB"
                     /locus_tag="PXD71_001286"
     CDS             9883..10965
                     /gene="leuB"
                     /locus_tag="PXD71_001286"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDL6291410.1"
                     /translation="MQTYNIAVLAGDGIGPEVMAEAIKVLEATQQKFGFKLNFNQYNI
                     GGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKWTHLLPDEQPERGSLLPLRKHFS
                     LFCNLRPAALYQGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGRDGEGAQTRA
                     FDTEVYYKYEIERITRVAFESAMKRKKHVTSVDKANVLQSSILWRETVTEVAKEFPEV
                     TLEHMYIDNATMQLIKAPESFDILLCSNIFGDIISDEAAMITGSMGMLPSASLNEQGF
                     GLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLGDAADAIETAIQKALADGYR
                     TADLADDSKPLSTSEMGDIIAKNILA"
     gene            complement(11052..11447)
                     /gene="msrB"
                     /locus_tag="PXD71_001287"
     CDS             complement(11052..11447)
                     /gene="msrB"
                     /locus_tag="PXD71_001287"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL6291411.1"
                     /translation="MIKPIHALTEQQVDILINHGTERPFTGKFLNEHRVGIYRCARCH
                     NDLFHSDTKFDAGCGWPSFYQTISESSLRYLDDYSLGRHRIEIRCGNCDSHMGHVFPD
                     GPPPTGLRFCLNSAALNFKWEETGEEIDG"
     gene            11672..12676
                     /gene="gap"
                     /locus_tag="PXD71_001288"
     CDS             11672..12676
                     /gene="gap"
                     /locus_tag="PXD71_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL6291412.1"
                     /translation="MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVDYMAYM
                     LKYDSTHGRFDGTVEVKDGQLVVNGKAIRVTAERDPANLKWNEIGVDIAVEATGLFLD
                     DETARKHITAGAKKVVLTGPSKDATPMFVNGVNFDTYAGQDIVSNASCTTNCLAPLAK
                     VIHEKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPALNGKLTGMAFRVPTTNVSVVDLTVNLEKPATYAEICAEIKRASENEMKGVLGYTE
                     DAVVSTDFNGATETSVFDAAAGIALTDTFVKLVSWYDNEVGYSNKVLDLVAHVYNYKG
                     "
BASE COUNT         3985 a         2242 c         2752 g         3830 t
ORIGIN      
        1 tcaattatta actgctaagc cgattaataa agaagtgtta aatcaagtac ttaaaacggt
       61 atttgataat gaacatttgc tagggattga tttacaagta tatggggctg aatattggaa
      121 tgtatcaata gacaataaac caagtttaga ttgggtttct gctgaaattg caatagaaaa
      181 tgaaaaattg ggtatcttaa gctggaaacc ctcactggtt tgtcctggtg aacgattgat
      241 ccaaaatgtg tcggaaatga ttggtcgtag cctttatgta gtgcagatgc aaaagcaaca
      301 gcaacaattg gtactcatgg aggagcgtgc aattattgct cgtgagttac acgattcctt
      361 agcccaatct cttacgtttt tcaaaattca ggttagcctg cttaagcttg ctaaaacaga
      421 tgaaaagcgt aatgaaattt taactgtatt tgaaaaagcg ttaaatgatg cttacagcca
      481 attaagggag ctgttgacga cgtttaggct aacgattcaa gaagcgaatt tacaacaagc
      541 gttagaaaaa attttagaaa gcttaagacc gaaaacctca gcacagattc gcttaagttg
      601 caaattacct tcgcatatgt ttaatgctca gcagcaagtt cacgtattgc aaattgtacg
      661 agaagctgtg attaatgcta ttaagcattc gcaagccact gaaattaatg tgattgctga
      721 aacaaatgat gatggtgaac attgtttgac tattcaagat aatggcgtag ggcttgctgc
      781 ccatacgcaa ccagaagggc attatggact gacgattatg caagaacggg cggctgaact
      841 caaagccgaa ttttcgattg aaaatgtgga aagtggcgga gtaaaagtac aggttttatt
      901 gccgagtatg attagcaagc ggtaataaaa tattaatttt ttgcgtatta ttggcaacga
      961 ataaaaggat aacaaatgca attccgaaaa gcagtagaag cggattttga gacaatattg
     1021 gcaatttata atcaagcaat tcccactcac caaattaccg ccgatttaga actggcaact
     1081 acgcaaaatc ggcggaagtg gttcgatttt caccttgaaa cagcccaata tcctatttgg
     1141 gtggtcgaag atgaaaaagg cattgctggc tggtttagtt tttcaccatt ttatgaaaga
     1201 ccggcatttt tacatacttc ggaaatcagt atctatttag accgctattc caaaggaaaa
     1261 ggctatggct caaaaattat tgagtttatg caagctgaaa tgcttaatca caatattcat
     1321 acgttgatgg cgtatgtgtt tgaattaaat acagtgagcc aaaatctaat gagaaaacac
     1381 ggctttgaac aatggggaag atttccgaat attgctaata tgggaaaaga tgagaaaggt
     1441 gaggataaat ggcgaacgtt gctgatgatg tcttaccaaa aaggtatcga ataacaagcg
     1501 gtgatttttt agaatatttt tgcaaataag gacaatatgg aaaagacaaa aattgtactt
     1561 gcaacaagta atgcagataa agttaaagaa atggcagatg tactctcaca atttggtttt
     1621 gaggtggtgg ctcaaagtga atttgggatt gaatcaccgg aagaaaccgg tttgactttt
     1681 gttgaaaatg cgctgattaa agcacgattt gctgctaaaa tgacaggttt accggcgatt
     1741 gccgatgatt caggtttatc ggttatggca cttggtggtg aaccggggct atattctgca
     1801 cgttatgccg gagagcaggc aaccgatgcc gataatcgcc aaaaattact agccaaaatg
     1861 caagggcaag agaatcgtct ggcgaaattt gtcagttgta tcgtgttttt aaaacacgaa
     1921 actgacccaa caccttatat tgcgtcgggt gaatgtttcg gtgaaatttt gacggaagaa
     1981 agaggtgaaa atggtttcgg ctacgattcc ctgtttttct acccaccgaa agcttgcact
     2041 tttgccgaat tagaaaccaa agagaaaaaa gcgatttcac accgtgcaat cgcactagag
     2101 caattaaaac agcaattagc aggagagaat aaatgattat tacaactaca cctacgatag
     2161 agggtaaaca aattgtagaa tacaaagggt tagtatttgg cgaagtagtt tctggggcta
     2221 attttatccg tgatttctta gccggtatca ccgatattat tggcggacgt tcaggtgctt
     2281 atgaaagcaa attaaacggt gcaagaaaag aggcattagc tgaattagct aaggaggcac
     2341 aaaaagtggg ggcaaatgcg gtggttggcg tttctatgga gtatcaatca atgggcggcg
     2401 ataaaggaat gttcatcgtg gtagcgactg gtactgcggt tgtaatacgt taatttatgc
     2461 agtttgaaca acactttgaa gcaaatttct ggcagaacaa atcgctactt gaaatgaatg
     2521 atgctgaatg ggaagcatta tgtgacggtt gtggtaagtg ctgctaccgg aaatatattc
     2581 agggaagagg gcggcgtgaa cggctttact atacctcggt tgcctgtaat ttattagatg
     2641 tgaatacagg ccgttgtatg aattactcga accgttttaa aattgagtcg gattgtacaa
     2701 aattaactaa aaagaactta cctgattttg gctggttgcc aaaaacctgt gcttatcgtt
     2761 tactttatga aggaaagcct ctttttgatt ggcacccttt aatctctaaa gatcctgatt
     2821 ctgtaaaaaa tgccggaatg ctgattgcta acggtattca cgaaaaagat gtgattgatt
     2881 ggtttgagtt tgttattgat gaagtatgag aaaaaggcgt ttggtgcaaa cgcctttttg
     2941 ttatagagat taggcttttt ccatctcaaa ttcaattaac atcatcaaaa tatgaatcac
     3001 tttaatgtga atttcttgga tgcggtctgc gtaacggaaa tgaggtactc ggatttccac
     3061 atcggctaat cctgccattt gcccgccatc tttgccggtc attgcaatta ctttcatgcc
     3121 tttttctttg gcaactttga tcgcatttaa cacattttta gagttaccag aggttgatag
     3181 accaaagagt acatcgcctt tttgcccaac ggcctctaga taacgagaga agacatattc
     3241 ataaccgaag tcattactta cacagcttaa atggctgaca tcggaaatag cgattgccgg
     3301 gtatccggga cggttttcac ggtaacgacc tgttaattct tcggcaaagt gcatcgcatc
     3361 acagtgcgaa ccgccattac cgcaagaaat caccttacca ccttgcttga aactatttgc
     3421 aatcagcatg gcggcatctt gaattaattt aatatttttt tcatcagaca taaatttgtc
     3481 taatacatct gcagcttctt gaagctcagc tttaatttgt tctaaataca tcatttgctc
     3541 ctttgtttgc aaaacgctta cctttaaaca tcggctttac caatgggggt agcgagtaaa
     3601 aaattcgttt tttatgaccg cttgaggcat aaaaagtgca ataagtttag aagatctatg
     3661 ctgtattgta aacctaataa cttaaataat ctgaataggg ttgtaaacaa aagtttccaa
     3721 aagagaaaaa tagcggtatg atcgcctcac aatccctctc gcattgttaa ctctagtagc
     3781 taatatcagg ctttaagcgt attaaacaat gcacaaattt gctatttata ggagactata
     3841 tggtcgtaaa tccagaatct tttgaacaga taattggaca atctgtaaag ataaaagagg
     3901 ttgttgaaca agcaaaaaaa tttgccaacc ttgatgcacc attattgatt caaggtgaga
     3961 cgggaacagg aaaagattta tttgccaaat cttgtcacca ttttggatct cgtcgaatgc
     4021 aaaaatttat tgccgtgaac tgtgccggtt taccggctga agaagcagaa tcggaaatgt
     4081 tcggacatcg tggcaatggc gttgagtccg tcggtttctt tgaatatgct cacggtggta
     4141 cagtattatt agactctatt tctgagttat cgctggaaat gcaagccaaa ttattgcgct
     4201 ttttaaatga tggctctttt cgtcgagtgg gcgaagatca agagattcaa gtcaatgtta
     4261 gggtgatctg tacttcatta cagcctttgt cattattagt tgaaaaaggc aaagtaagag
     4321 aagatctgtt tcatcgttta aatgttctca ctttaaattt accacctttg cgagatcgaa
     4381 aagaggatat tccattatta gctgattact ttatcgccca aattagcaat cagttggggg
     4441 tgagcaaatt agagtatgat aatgtttttt tgcaagcatt gcagaattat cactggcgag
     4501 gcaacttaag agagctttat aacgcacttt atcgagcttg tacactttcg catagctacc
     4561 gtttagcggt aaaagatctt catttaccca gagatatgga ggcgatgatt gaagtacgtg
     4621 aggaggcgac acttgaggaa atgatgagcc agtatgaagc ccaattattg caccaatttt
     4681 atgcagaata tccgagtacc cgaaaattag cacaacggct cggactttca catactgcgg
     4741 tggcaaacaa attgcgtcaa tatgggttaa caaaaaatgg aaacagtaaa gatgaataaa
     4801 ctagcggtca ttttttcagt atgttttgca aatttagggc taaattcgac cgcttacgca
     4861 gcgccaagta ttcctcaaga gctacttgaa aatagccttg tgtattgcac tacttcatcg
     4921 ggattctctt ttaacccaca aaaatccgat gtgagttcga atatgaatgt ggtaacagag
     4981 cagatctacg ataaattagt tgagtttaat ccgaaaaaaa atcgactaga gcctgcttta
     5041 gctgaaagtt attcaatcag cgacgatggt ttagtgatta cattacgttt aagaaaaaat
     5101 gtcgcttttc atacaactcc atggtttact ccgagtcgta aattaaacgc agaagatgtg
     5161 gtgttttcgc taaaacgaat gattggtaaa gaaagtgatt tacctgaagt gaaaagtgag
     5221 cctgaaagtg tattacacca ccagcgacaa agctatattt accaaaaaac agcggggcag
     5281 atttattttc cttattttga aagtgttgat ttagcgaata aaattgccag cattcattct
     5341 ccgtcaaatg acattgtaaa aattgaatta gtccgtcctg attcagcatt gttggcgcat
     5401 ttagccagcc aatatgcagt tattctgtct aaagaatatg ctttacagct caatgcagat
     5461 gataacttag ttcaactgga tttattaccg gtaggaacag gcgtatatca acttgaaagt
     5521 tatgaaaata atgattatgt gcgtttaaaa ccaaatatga attactgggg caaaaaagca
     5581 tatattcaaa atatggtcgt agatttttcc acagaagcga ccggcaggat ggcaaaattt
     5641 ttaaataatg aatgtgatgt ggttgctttt ccggaaccta gccaattatc agtgttaaat
     5701 aatcggcagc ttattagtag taaaggtgct aatttggttt atttagcatt caatatgcaa
     5761 cggggtattg ggcaggattt ggcattacgc caacgtattg cacgcggcat aaatcgcaaa
     5821 cgtattgcag agaaattatt ttatggtaca ggtgaagtag cggataacct attaccaaca
     5881 gctttgtggt cgaaaaaaaa taatggaagc tacccttatc aaccacacca aaattcaggt
     5941 gagcaagcgg ttgaaatgca tgacagtatt ataaatcggt ctttagatag caaaacagag
     6001 accgaagaag taaaccaaaa acagcgctta aaactgtggg taattgatga aaaacgagtt
     6061 tataatcaac acccattaaa aatggcagaa atgatacgtg ctgatttagc tacgcaaaat
     6121 ttagaggtcg aaattcgcgc agtaagtcga gcttatttgg tgcaacagct tgctaataaa
     6181 agtgcggatt atgatttgat tttaggtggt tggttagcaa ataatcttga tcctaacagt
     6241 tttttggagg ccttactcag ttgtaaagct gcggaaacgg tgactaattt atcaaattgg
     6301 tgcaatgaag attttgattt ttggttacaa acagcccgtc taagtgatga tccggcatca
     6361 gctaatttat tgtatgaatt tgcacaaaaa ttattagaag agcaattgcc tattctccct
     6421 ttaatgaatg ctaatcgtgt attagtcata aaagatggag tgaaacaggc agatatttcc
     6481 cctttcggcc aggtaaaatt atcagagtta cgtttgggct tggggcataa aaaaggaaac
     6541 taattatgct gattgtcttt tttcgaaaaa tttttctaac cttgattacg ttgatttttt
     6601 tgtcacttat cagttttaat attctgttac gagatccatt aaaccactta gccgagttag
     6661 gctggtttga ggcttattgt tattatgtaa aatctctgct aaatggtgat tttggtatta
     6721 gtttcagtaa tggagaaccg ctcacagcgc aaattttaca ggtgttcccg gctacaatta
     6781 cgctttgttt atcggctctc attgtttctt taattattgg tttgcctctt ggtttttttg
     6841 cggctactca gcaaaaaaat atggttgggc gattattaaa tacactaggt tcattgagtt
     6901 tagccgtgcc ggtattttgg ctggcattag tgctaatgta ttacgcttct ctcaatcagt
     6961 gggaagtgtc agcaattgga gaaatccatt cgctttatcc gaaacagatg gtaaccggct
     7021 ttttgctatt agatatttgg ttatctgatg taccttataa attaaaaatg atgcagagtg
     7081 ctttacatca cttagctttg ccgacattag tcttggctgt gccagctaca ttagaagtta
     7141 tgcatattac tcaagctaga gcaggttatg taatgaaaca aaattacatt aaaatagcga
     7201 aaacgcgtgg ctggacaccg tttagagttt ggagaacgca tattatttgt aatactttac
     7261 ctgcattatt acctatgatt gcccgtacct ttattttaat ttttgccttc ggtatgttaa
     7321 ttgagaacat atttagctgg ggaggaattg gacgttggct gataaatgcc cttgctattc
     7381 aagactataa tgcgatttca gcgggagtag ttgcaatcgg ggtgtttgtg ttattagtag
     7441 atatggttac aggctttgtg cgggtgatgt tagacccatc agataaaaag gattggtatg
     7501 gcagttatta aagaacccga gcaatttaga gaaaccaacg catttaaggc attttggttt
     7561 catttccgac aagataaact tgcacttggc agtttttatc tttttttgct attactggct
     7621 ctcatttttg ttggtagttt gcttgctcct tacaattata atgaacaatt tgttggttta
     7681 gaattaatgc ctccctcttg ggacgacaaa ggtcaaatca gccatttttt aggcactgac
     7741 gatttaggac gggatatttt aagccgtatt atgtatggtt tttactacac gcttggttcc
     7801 gcactgatga ttaccttatt aattgcgatt atcggcggta tgatcgggat ttatgccggg
     7861 ctgaaaaaag gacgttcatt ttggttgatt agccatttgt tcgatacctt tttatttatg
     7921 ccgattttat tgattgcgat catcattgca accttaatgg aagcgagttt ggtcaatgca
     7981 atgttggcga ttttcttagc gatgttgccg cattttatcc ataaaattta tcaagcaacc
     8041 gaaaaacaac tcaaaaaaga gtacatcatt accttaaagc tagacggtgc aagctgctgg
     8101 tatttaattc gagaagtgat tattcctaat cttaccggcg tggcaattaa agcactttca
     8161 catatttttg tgatcgccat attagatatt agtgcgttaa gttttattat gctgggggct
     8221 aaaagcccga cccctgaatg gggagcgatg attcgtgaat ccatggaact gatttatata
     8281 gctccttgga cggtgatatt acctggctta gcaattattt ttagtatttt taccgttaca
     8341 atacttggta cacgcttaag cgtgattttt gaaaaatacc gtaaagaata gtatttaaat
     8401 aggatactga cacataacaa gcggtcattt ttaagtaaaa ttttacagat taaggaatag
     8461 tatggcatta cttgatattc gccatttaag tattgaaatt gatatgccaa aaggcagagt
     8521 aaaaatggtg gataacatca acctgactgt tgatgagggc gaaatttgtg gcttggtggg
     8581 agaatctggt tcgggaaaaa gcctcatagc caaagtgatt tgcggtatga ttaaagatga
     8641 atggattgtg agtgcagatc gctttcgctt tgacgatatt gaattactga aactttcgcc
     8701 agcggaacgc cgaaaattgg taggcgatca tatttcaatg gtattccaag atccgcttac
     8761 tagccttgac ccaagccagc aaattggtaa acaactgatg caaaccattc aatttagagg
     8821 gaagtggtgg caacgatttg ggtggaaaaa gaaaaaagca atcgagctac tacaccgtgt
     8881 tggtattcgt gatcatcaag atattatgaa aagttaccca agcgatatta ccgaaggcga
     8941 agctcagaaa gtgatgattg caatggcggt ggcaaatcag ccccgattat tggtggcaga
     9001 tgagccgact aatattatgg aatcaaccac gcagttgcag gtttatcgct tgctttccag
     9061 tatgaatcag aatttgggaa cctcaatctt gctggtgagt aatgacatgc gtagcattca
     9121 aaattgggtt gattcttaca atgtgcttta ttgtggacaa accgttgaaa ttgcggataa
     9181 agagagcatt cttgaatcgt cttaccaccc ctatactcga gtgttactca atagttcgcc
     9241 ggacttttct cagcctttag cctttaaaag tagcttaaat acgctacgag gctcagtccc
     9301 gatgttacaa gaaatgccga ttggttgtcg tttaggccct cgttgccctt ttgcacaaaa
     9361 aaaatgtatc aacaaaccgc cattagtgaa agtaaaacag cacgagtttg cctgccattt
     9421 tccgattaac tttagagaac aaaatttgct taaaaataaa acggaaaata cgccgtttgt
     9481 ggtggctgaa atagcggaaa aagaggcgtt ttaatgtggt tattattggc taagtgtaac
     9541 gggtaacatt aaagtatcta atacgaaact caagggtaaa tcgatcaccg caagcaccgc
     9601 gaatataccg ccttcttgca ttacttggaa atcttttatt acgccggcat aaacacggta
     9661 atcctgttca accaagctta gtactgtgcc acaagcggtg aggttaaaca tcaaatagag
     9721 tagaaaaacg agtttacaag cggtcaattt catcaaaaaa tctccaaata cgcttttatt
     9781 ttacgctatt tttatcgggt tggcgtaaaa ttcgttttaa aaacagctat tttcaggcag
     9841 aatgtagccg aatcgacatt aaatatacta agaggatctt atatgcaaac ttacaacatt
     9901 gcagtgcttg ccggtgacgg cattgggccg gaagtgatgg cagaagctat caaagtgctt
     9961 gaagcgactc aacaaaaatt tggctttaaa ctaaatttta atcaatataa cattggtgga
    10021 gcagcgattg atgctcaagg taaagcgtta cccgaaaata ccttaaaagg ctgtgaagag
    10081 gcagatgcga ttttgtttgg ctcggtaggt ggcccgaaat ggacgcattt actacctgat
    10141 gagcaacctg aacgaggttc gctattacca ctgcgtaaac atttctcgct attctgtaat
    10201 ttacgccctg ctgcattgta tcaagggctg gaaaaattct gcccattacg tgccgatatt
    10261 gctgctaaag gttttgatat ggtggtggtg cgtgaattaa ccggcgggat ttattttgga
    10321 cagccgaaag gccgtgatgg tgaaggtgcg caaacgagag cgtttgacac cgaagtatac
    10381 tacaaatatg aaatcgagcg tattacccgt gtggcgtttg aatctgcaat gaaacgtaaa
    10441 aaacacgtaa cctctgtgga taaagccaat gtgttacaaa gctcgatttt atggcgtgaa
    10501 accgtcacgg aagtagctaa agagttccca gaagtcacgc ttgagcatat gtatattgat
    10561 aatgcgacaa tgcagctaat caaagcacct gaatcgttcg atattttatt atgctcaaac
    10621 atcttcggcg atattatttc cgatgaggct gcaatgatta caggttctat gggcatgttg
    10681 ccttcagcca gcctcaatga gcaaggtttc ggcttatatg aaccggcagg cggttcagca
    10741 ccggatattg ccggtaaaaa tattgcgaat ccgattgcgc aaattttatc agccgcaatg
    10801 atgttgcgtt atagctttaa cttaggtgat gctgctgatg cgattgaaac agcgatccaa
    10861 aaagcccttg ctgatggcta ccgtaccgct gatttagctg atgacagcaa accgctttct
    10921 acctcagaaa tgggcgatat tattgcgaaa aatattctcg cttaaggcta gaagttaaat
    10981 agcacaagcg gtcattattc gccaaatttt tgcaaatttt ccgctaataa tgaccgcttg
    11041 tttattactc gttagccgtc aatctcttcg cctgtttctt cccacttaaa attcaatgcc
    11101 gcagagttga ggcaaaaacg caaccctgtt ggtggtgggc cgtccggaaa aacgtgtccc
    11161 atatgcgaat cacaattccc acaacgaatt tcgatacgat gacgtcctaa actgtaatca
    11221 tctaagtaac gtagtgacga ttctgaaatc gtttggtaaa aacttggcca gccacaaccg
    11281 gcatcaaatt tagtatctga gtgaaataaa tcgttatggc atctggcaca acgataaatt
    11341 cctacccgat gttcattcaa aaattttccg gtaaacggac gttccgtacc gtggttgatt
    11401 aaaatatcaa cttgttgttc tgtcagggcg tgaataggtt taatcatcag caattctcct
    11461 aagatagtag cgaagtcatt gtagcttttt ttgacctgaa aatcgttata aatttcaaaa
    11521 tttgacatag atcacaaaaa atttatctgt tatattggaa gggttctttt ggcttgtcag
    11581 gaaagtgaaa agatgtgcta taattcaagc atctttagtg tagtacataa agaacaaatt
    11641 ttgtttaact ttaatatagg tagaaaaatc tatggcaatt aaaattggta ttaacggctt
    11701 tggccgtatc ggtcgtatcg ttttccgtgc agcacaacac cgtgatgaca tcgaagtggt
    11761 aggtatcaac gacttaatcg atgttgatta tatggcttat atgctgaaat atgattcaac
    11821 tcacggtcgt tttgacggta ctgttgaagt taaagacggt caattagtag ttaacggtaa
    11881 agcgatccgc gtaacagctg agcgtgaccc ggctaactta aaatggaatg agatcggtgt
    11941 tgatatcgca gttgaagcaa caggtttatt cttagatgat gaaactgctc gcaaacacat
    12001 caccgcaggt gcgaagaaag ttgttttaac cggtccgtct aaagatgcaa cacctatgtt
    12061 cgtaaacggc gtaaactttg atacttacgc aggtcaagac atcgtgtcta acgcttcttg
    12121 tacaactaac tgtttagcac cattagcgaa agttattcac gaaaaattcg gtatcaaaga
    12181 aggtttaatg acaactgttc acgcaactac tgcaacgcaa aaaacagtag atggtccatc
    12241 agcgaaagac tggcgtggtg gtcgtggcgc gtcacaaaac atcattcctt catcaacagg
    12301 tgcagcgaaa gcagtaggta aggtattacc tgcattaaac ggtaaattaa ccggtatggc
    12361 tttccgtgtt ccaaccacta acgtttctgt tgttgattta actgtaaact tagaaaaacc
    12421 agcaacttat gcagaaatct gtgctgaaat caaacgtgct tcagaaaacg aaatgaaagg
    12481 cgtattaggc tacacagaag atgcagttgt ttcaacagac ttcaatggtg caactgaaac
    12541 ttcagtattt gatgcagcag caggtatcgc attaactgat actttcgtta aattagtatc
    12601 ttggtacgat aacgaagttg gctactcaaa caaagtatta gacttagttg ctcacgtata
    12661 taactacaaa ggttaattgc taagttatat tttaaatata gagccaaatt ctgaacggaa
    12721 tttggctctt ttgctgcgaa caataaattg aatatagtcg cgttttccca taaaatcaat
    12781 ttcctacaaa caggaactaa gcacagtaa
//
LOCUS       DANLXS010000063        12469 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.063, whole genome shotgun sequence.
ACCESSION   DANLXS010000063 DANLXS010000000
VERSION     DANLXS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.063"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(196..378)
                     /locus_tag="PXD71_001289"
     CDS             complement(196..378)
                     /locus_tag="PXD71_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291413.1"
                     /translation="MYQFGYLRTKIGTDTQRAMLWNCPTYVLIIPFLLNTSVKKQYHA
                     FWQKYHQHNNDVSFPY"
     gene            528..1568
                     /gene="selD"
                     /locus_tag="PXD71_001290"
     CDS             528..1568
                     /gene="selD"
                     /locus_tag="PXD71_001290"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:573..575,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDL6291414.1"
                     /translation="MSEPIKLTQFSHGAGUGCKISPKVLGTILQTQLEQFVDPNLLVG
                     NETADDAAVYDLGNGQAIISTTDFFMPIVDAPFDFGRIAAANAISDIYAMGGKPIMAI
                     AILGFPVNKLPAEVAQQIVEGGRFACQQAGIALAGGHSIDSPDPIFGLAVTGIVPTER
                     VKRNASAVAGCELFLTKPLGIGILTTAEKKGLLKPEHQHLASEVMCQINLIGSAFSEL
                     PEVTAMTDVTGFGLLGHLSEVCQGSGLRAEVDFEDIQVLDGTKEYIALGAVPGGTNRN
                     FDSYGHLISAMTDEQKAILCDPQTSGGLLVAVLPQAVEKVQQIANRANVPLFHVGRLL
                     EMEQGKPRIEVI"
     gene            1568..2041
                     /locus_tag="PXD71_001291"
     CDS             1568..2041
                     /locus_tag="PXD71_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL6291415.1"
                     /translation="MFKPNVTMACVVHCKGKFLFVEEIEYGKRTLNQPAGHLEKDETL
                     LEGASRELFEETGIRAEMANLVKIYQWQAPRSQTDFLRFLFSVELEEWLEPNPQDADI
                     TQALWLTLAEFEDYIQQQGQCARSPLVVQSVKDYLSGERYPLSLLNAFEWARNGK"
     gene            2031..3239
                     /gene="ubiH"
                     /locus_tag="PXD71_001292"
                     /gene_synonym="visB"
     CDS             2031..3239
                     /gene="ubiH"
                     /locus_tag="PXD71_001292"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDL6291416.1"
                     /translation="MENKTTTQFDVVIVGGAVTGSVLALALSSFTHHKISIAIVEKSL
                     PDYEQQGGFDARSIALAEGSLQKMGQIRPLAGSSLGEMISEIGTPIQQIQVADKGHFG
                     KTTLKASEQNVAQLGMVVELAKFGRKLTACLAQQANITLFCPNQVVGIERSQQHCGLS
                     LKNGEKLSCSLLVAADGIQSQIAKQCGVETVKVRDYAQSAIIANVELSESHQNQAFEY
                     FTPQGPFALLPLSGNMMSLVWCVKEPNELIQLSDSEFLARLQQQFGWKLGQFVRVSKR
                     FVYPLILQKAQSHIHHRLAVVGNAAQMLHPVAGQGFNLGLRDLTTLAELVALAFNQGK
                     DIGEFSLLSTFENERTQDQARMINATSGLISLFCCEMLPVQVVRNLGLLTVSHSKIAR
                     DFVANQALGW"
     gene            3502..4734
                     /locus_tag="PXD71_001293"
     CDS             3502..4734
                     /locus_tag="PXD71_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HDL6291417.1"
                     /translation="MTTENMSTPKQAFIGLQMLFVAFGALVLVPLITGLDPNVALLTA
                     GVGTLIFQICTKRQVPVFLASSFAFIAPIQFGVQQWGIPVTMGALLCSGFIYMALSAL
                     VKAKGVNILHKYFPPIVIGPVIMVIGLGLAPVAVDMATGKSANIAYNTAIIVSMITLV
                     TTLIVAVFAKGLLKLVPILAGLTVGYILSVFMGLVDFTKVVEAPWFSLPKVSAPEFKL
                     DAILYLLPIALAPAVEHLGDVMAISQVAGKDFMKKPGLHRTLLGDGLATSAAAALGGP
                     PNTTYSEVTGAVMLTKNFNPKIMTWAACWAIAFSFCGKIGAFLATIPTFVMGGIMMLL
                     FGAVAVVGINTLIKAKVDLSIPRNLCIASVVMTFGIGGMLINIGEFSLKGISLCALVA
                     IVLNIVLPKEKVEDSAAH"
     gene            4801..5787
                     /locus_tag="PXD71_001294"
     CDS             4801..5787
                     /locus_tag="PXD71_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL6291418.1"
                     /translation="MQKLTFSPPLLNTPCPWCSELESLRELYACEYTGAVTTRTSMPQ
                     PYPHDWAKNQYVLFDSNAQKTASVNTQDATSLQTASLNTIGLSPNNLDTTISFVRTIS
                     NELTAPSSKPFIISVFGSPEEVGECYEKIVAFQTEVKMPLAMEINISCPNIPGEISPA
                     YSAEELSHYLHALQTSLKKLAQGDNTLPIGIKIPPFTYQNQYDELIKGLLQAVENGEN
                     LPISFITSTNTLGSSLVLNAGKAVLNSEVGTGVGGMAGTAIHALSLGNVYTIRQMLDQ
                     EPKLKHLQIIGVGGVNDKAGFERMKSVGADFVGLATALGVKGIGVFKEILSE"
     gene            complement(5828..6238)
                     /locus_tag="PXD71_001295"
     CDS             complement(5828..6238)
                     /locus_tag="PXD71_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HDL6291419.1"
                     /translation="MLYSVLTAHILLNEPYRNGAHSTQHEHLVVMFEIDEESGYFYAM
                     DLHQTEQPVVDSLFVFRVSDIEQNSLKEPRRLEICWSEDGYQAFLLINGYPHAVFDFR
                     QFVGYNHTKFPMPELGSMWVHKETTNELVEKWLG"
     gene            complement(6304..7614)
                     /gene="eno"
                     /locus_tag="PXD71_001296"
     CDS             complement(6304..7614)
                     /gene="eno"
                     /locus_tag="PXD71_001296"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL6291420.1"
                     /translation="MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTG
                     SREALELRDGDKGRFLGKGVLKAVSAVNNEIANALVGKDGSAQAEIDQIMIDLDGTEN
                     KSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
                     DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKAKGLNTAVGDEGGFAPNLAS
                     NADALACIKEAVEKAGYVLGKDVTLAMDCASSEFYDKERNVYDMKGEGKTFTSQEFTH
                     YLEGLCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDLFVTNTKILKNGIEK
                     GIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQ
                     IKTGSMSRSDRVAKYNQLIRIEEALERAGTPAPFNGRKEIKGQA"
     gene            complement(7805..8983)
                     /gene="rlmC"
                     /locus_tag="PXD71_001297"
     CDS             complement(7805..8983)
                     /gene="rlmC"
                     /locus_tag="PXD71_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDL6291421.1"
                     /translation="MSSLLHCSHFERGDCTSCQWIEKPYSTQLAAKEADLKRLILPFI
                     LQNKTEILPLVGSPISQFRNKAKMVVSGSVERPILGILKDQNDAQSGVDLTDCLLYPS
                     SFHRLFPILKDFIARAGLVPYNISKKKGELKYILITESQYNQSFMLRFVVRSEAKRAL
                     VERELPELLEKLPPNSVVSLNIQPQHAALLEGETEIFLTEQKVLEEHFNGIPLFIRPQ
                     GFFQTNPVVASQLYHTAQSWIEHLPIAKLWDLFCGVGGFGLHCANALQAKNSNVELTG
                     IEISASAIASATKSAEKLGLKQVKFASLDSAQFALNEAGSPPDLVIVNPPRRGIGKLL
                     AEFLNQLGSPYLIYSSCNAVSMANDFTFLTNYELHKVQLFDMFAHTAHYETLTFLVKK
                     "
     gene            complement(8976..10013)
                     /gene="nagZ"
                     /locus_tag="PXD71_001298"
     CDS             complement(8976..10013)
                     /gene="nagZ"
                     /locus_tag="PXD71_001298"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDL6291422.1"
                     /translation="MLLIDIKDKELTQEEVEMLEHPLVSGLILFTRNFYDKAQIQALV
                     KSIRQRVKKPLLITVDQEGGRVQRFREGFTKLPAMQSFNQLATTPEQAMQLAKETGWL
                     MAAEMFALDIDLSFAPVLDLGHECKAIGDRSFGQEPAAILPVAEAFIDGMREIGMATT
                     GKHFPGHGHVLADSHLETPFDDRPKERIFNHDILPFKQLIAKGKLSAIMPAHVVYTQC
                     DSQPASGSSYWLKEVLRKQLNFNGVIFSDDLGMKGAGFMGNFVERSEKAIQAGCDLLL
                     LCNEPEGLVQVLDGLKYQPTQAQKERRLSLMKRKTVTWRELENSSRYQQTQAELTALQ
                     NNWLEWKEANV"
     gene            complement(10139..10870)
                     /locus_tag="PXD71_001299"
     CDS             complement(10139..10870)
                     /locus_tag="PXD71_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDL6291423.1"
                     /translation="MFDWITNPEAWVALFTLTGLEIVLGIDNIIVISILVSRLPIEQR
                     QSARIIGLGLAMGTRILLLLSLAWMMRLVEPLFTIGNNPISGRDLILLIGGLFLIVKS
                     AMELKESISAESHEEKSESIKKASFFMVLIQIAIFDIIFSLDSVITAVAMADDIPVMV
                     IAIIIAVAVMMLAAKTIGDFVDNNPTIKNLALAFLILIGIVLVGEGFDIHIPKAAVYT
                     AMGFSVVVEMLNIKMRRNQAKLEQA"
     gene            complement(10959..11639)
                     /gene="mutH"
                     /locus_tag="PXD71_001300"
     CDS             complement(10959..11639)
                     /gene="mutH"
                     /locus_tag="PXD71_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDL6291424.1"
                     /translation="MQLSTFSPTEQALLSKAEWLAGFTLGEIAEMLHIPIPADLKRDK
                     GWVGTLIETALGAKAGSKAEQDFAHLGIELKTIPVNQKGLPLETTFVSLAPLTQNNGI
                     IWETSHVKHKLSRVLWIPVEGERKIPLAERHIGQPILWSPTITQESRLQQDWEELMEL
                     IVLGELHKINATLGEVLQLRPKGRNNRSITSAINAKGEIVQSLPLGFYLRKHFTAEIL
                     QNFLHCPL"
     gene            complement(11731..12366)
                     /gene="lexA"
                     /locus_tag="PXD71_001301"
     CDS             complement(11731..12366)
                     /gene="lexA"
                     /locus_tag="PXD71_001301"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDL6291425.1"
                     /translation="MRKHLTARQQEIFDFVKNHIETTGMPPTRVEIAREIGFKSPNAA
                     EEHLKALARKGYIEMLSGTSRGIRILVEDENQAANDEDEGLPLIGKVAAGTPILAVEH
                     VENHYPVNGAMFNPSADYLLKVNGNSMEKIGILDGDLLAVHKTNTARNGQVVVARVND
                     EVTVKRLEKKGELIYLHPENDELKPIVVNPAQEYIEIEGIAVGVIRSNAWM"
BASE COUNT         3488 a         2533 c         2772 g         3676 t
ORIGIN      
        1 gactgtcaga tcaagtctga tcttctacaa ctaagccttc tcagaataaa taaattttca
       61 caagcggtgc aactttgcaa aaacttcggt attatgtagc agttgcacaa aaccccaaat
      121 tcattaaaac cgcttaaaaa tgaaatgccc taaactgatt tttcagtcta gggcatttat
      181 tatcacatat tatggttaat agggaaaact cacatcattg ttatgctggt gatatttttg
      241 ccaaaatgcg tgatattgtt ttttcaccga tgtgtttaat aaaaacggga ttatcaagac
      301 ataggtcggg caattccata gcattgcacg ttgtgtatcc gtgccgattt tggtgcgtaa
      361 gtaaccaaac tgatacatta gacccaatcc aaccgtcacc tattctaata gcataataac
      421 taaggtcgca tcattaaatt aaaagctcat tttttgcaaa aaatttccca aattccaccg
      481 cttgttggcg tacaataggc ataattttga tttacgatgg aggcagaatg tctgaaccga
      541 taaaacttac gcagttcagc cacggggcgg gttgaggctg taaaatttcg cctaaggtgt
      601 tagggacaat tttacaaact caattagaac aatttgtcga tccgaattta ttggtaggca
      661 atgaaacggc ggacgatgct gccgtctatg atttagggaa cgggcaggcc attatcagca
      721 ccaccgattt ctttatgccg attgtggacg ccccgtttga tttcgggcga attgctgctg
      781 ccaatgcgat tagcgatatt tatgcgatgg gtggcaagcc gattatggcg attgcgattc
      841 tgggctttcc ggtgaataaa ttgccggctg aagtggctca acaaattgtc gaaggtgggc
      901 gttttgcgtg ccagcaagcc ggtattgcct tggcgggcgg gcattcgatt gattcgcccg
      961 atccgatttt cggcttggct gttaccggca ttgtgccgac cgaacgggtg aaacgcaatg
     1021 cctctgcggt ggctgggtgt gagctgtttt taaccaagcc tttggggatt ggcattctaa
     1081 ccacggcaga gaaaaaaggt ttgttgaagc ctgaacatca acatctggca agcgaggtaa
     1141 tgtgccaaat caacttgatt ggttcagcgt tttccgaact gcctgaagtc acggcaatga
     1201 ctgatgtaac cggttttggc ttactcgggc atttaagcga ggtgtgtcaa ggctcgggct
     1261 tacgggcgga ggttgatttt gaggatattc aagtccttga tggcactaag gagtatattg
     1321 cattgggagc agttccgggt gggacaaatc gcaatttcga cagctatggg catttgattt
     1381 cggcaatgac ggacgagcaa aaagcgattt tgtgcgatcc gcaaacctct ggcggtttat
     1441 tagtggcggt gctgccacaa gcggtcgaaa aagtgcaaca aattgcaaat cgggcaaatg
     1501 tgccgttatt ccacgttggc agattgctgg aaatggaaca gggcaaacca cggattgagg
     1561 taatttgatg tttaaaccaa atgtaacaat ggcttgcgtg gtgcattgta agggaaagtt
     1621 tctgtttgtg gaagaaattg aatatggcaa acgtaccctt aaccagccgg cagggcattt
     1681 agaaaaagat gaaaccttgc tggagggggc aagccgtgag ctgtttgagg aaaccggtat
     1741 tcgggcagaa atggcaaatt tggtgaaaat ttatcaatgg caagcccctc gttcacaaac
     1801 cgatttcttg cgttttctgt tttcggtgga actggaagag tggctggagc cgaatccgca
     1861 agatgcggat attacccaag ccctttggct tactttagca gaatttgaag actacattca
     1921 gcaacaaggg caatgtgcca gaagcccgtt ggtggttcaa tcggtcaaag attatttaag
     1981 cggtgagcgt tatccattaa gtttgttgaa tgcgtttgaa tgggcgagaa atggaaaata
     2041 aaacaacaac ccaatttgat gtggtgattg tgggcggggc agtgaccggc tcggtgctgg
     2101 cattggcgtt aagtagtttt actcatcaca aaatctcgat tgcgatagtg gaaaaatcgc
     2161 tgccggacta tgaacagcaa ggcggttttg atgctcgcag tattgcctta gccgaaggca
     2221 gtttgcaaaa aatggggcaa atcagaccgc ttgcaggctc aagtttaggc gagatgatta
     2281 gcgaaatcgg cacgccgatt cagcaaattc aagtagcgga taaagggcat tttggtaaaa
     2341 cgaccttgaa agcaagcgag cagaatgtgg ctcaactggg tatggtggtg gaacttgcaa
     2401 aattcggcag aaaattgacc gcttgtcttg cacagcaagc taatattacg cttttttgcc
     2461 cgaatcaagt ggttggcatt gagcgttccc aacaacattg tggattgagc ttgaaaaatg
     2521 gtgagaaact aagctgttcc ttgctggtgg cggcagatgg tattcaatct cagattgcaa
     2581 aacagtgcgg tgtggaaacc gtaaaagtaa gggattatgc acaatcggca attatcgcca
     2641 atgtggaact gagtgaatct caccaaaatc aagcctttga atattttacg cctcaaggcc
     2701 cttttgcttt attgcctttg agcggaaata tgatgtcgtt ggtgtggtgt gtgaaagagc
     2761 cgaacgagtt aatccaactt tcagacagtg aatttttagc ccgtttgcaa caacaatttg
     2821 gttggaagtt agggcaattt gttcgagtga gcaagcggtt tgtttatccg ctcattttgc
     2881 aaaaagccca gtcgcatatt caccaccgct tagcagttgt cggcaatgcc gcacaaatgc
     2941 ttcacccggt tgctgggcaa ggtttcaact taggcttgcg tgatttgact accttagcgg
     3001 aattagtggc tttggcgttt aatcaaggga aagatatagg cgaattttcg ctcttatcaa
     3061 cttttgagaa tgagagaacg caagatcaag ccagaatgat aaatgctaca agcggtctga
     3121 tttcgctttt ttgttgcgaa atgttaccag ttcaggttgt gaggaattta gggttattaa
     3181 cggtttccca ttcaaaaata gccagagatt ttgttgctaa tcaggcatta ggttggtaaa
     3241 aaacacaaaa gcagcaattt tctatttgac actgttttta aaaaggcgta ttatccaacc
     3301 cgtttaaaaa tctcaccaaa gccttgaatg caaattcaag gctttttcct ttttagtgaa
     3361 agcctatgaa ttttgtttgc cgtaaaatcg gcaaaaaaat tttgcctaaa attcgcaatc
     3421 gtttgcgaaa taggtttata gcgttgtttt catctgggta actgcaatgg ttacatcgca
     3481 atacatcaaa gagagtaaaa aatgaccaca gaaaatatga gtacgccaaa gcaggcgttt
     3541 atcgggctgc aaatgctgtt tgttgcattt ggggcgttag tattagttcc gctgattacc
     3601 ggtttagatc ctaatgttgc ccttcttacc gccggtgtcg gtacgttaat cttccaaatt
     3661 tgtactaaac gtcaagttcc tgttttcctc gcatcgagtt tcgcttttat cgctccaatc
     3721 caattcggtg ttcaacaatg gggaattccc gtcacaatgg gagcattgct ctgttccggt
     3781 tttatttata tggcattatc tgctttagtg aaagcgaaag gggtgaatat tctgcataaa
     3841 tacttccctc caatcgtgat tggaccggta attatggtga ttggtttagg tcttgctcct
     3901 gttgcggtgg atatggcaac gggtaaatcg gcgaatattg cctataacac cgctattatt
     3961 gtttcaatga taacgctggt aactacgctg attgtggcgg tgtttgcgaa agggctatta
     4021 aagctcgtgc caatattagc aggtttaacg gtgggatata tcttgtctgt ttttatggga
     4081 ttggtggatt ttactaaagt ggttgaagct ccttggttta gtctaccgaa agtttccgca
     4141 ccggaattca aattagatgc aatcttgtat ttattaccta tcgcacttgc tccggcagtc
     4201 gaacacttag gcgatgtaat ggcaatcagc caagttgccg gtaaagattt tatgaaaaaa
     4261 ccggggttac accgcacgct tttaggtgat ggtttagcaa cttcagcagc tgcggcatta
     4321 ggtggcccac caaatacgac ttactctgaa gtaaccggtg cggtaatgct aaccaaaaac
     4381 ttcaatccga aaattatgac ttgggctgcg tgctgggcta tcgctttctc attctgcgga
     4441 aaaatcgggg cattccttgc cactatccct acttttgtga tgggcggtat tatgatgttg
     4501 ctgttcggtg cagttgcggt tgtggggatc aatactctaa ttaaagctaa agttgattta
     4561 agcattccac gtaatctttg tattgcttct gtagtaatga cattcggtat tggcggtatg
     4621 ttaattaaca tcggcgaatt ctcattaaaa gggatcagct tgtgtgcatt agtcgctatt
     4681 gtacttaaca ttgtattacc aaaagaaaaa gtagaagatt ctgctgctca ttaatcaaag
     4741 gacgctgtgg cgtccttttt gctaataacc ctctatttgt aaataaaaag gaataacact
     4801 atgcaaaaac tcacattttc accaccactt ttaaatacgc cttgcccttg gtgttcggag
     4861 ttagagagct tgcgtgaact ttatgcgtgt gaatataccg gtgcggtaac aacccgtact
     4921 tcaatgcctc aaccatatcc acacgattgg gcgaagaacc aatatgtact ctttgattcc
     4981 aacgcacaga aaacagcaag tgtgaatact caagatgcaa ctagcttaca aacagcaagc
     5041 cttaacacca tcggtttaag cccaaataat ctggatacca ccatcagctt tgtacgcacc
     5101 atttcaaatg agctgacagc gccatcaagc aaaccattta ttatttcggt gtttggttca
     5161 cccgaagagg tgggggaatg ttatgaaaaa attgttgctt tccaaactga agtgaaaatg
     5221 cctttggcaa tggagattaa tatctcttgc ccgaatattc cgggtgaaat ttcgcctgct
     5281 tattccgcag aagaattatc acattacttg cacgcactac aaacttcgct aaaaaaactg
     5341 gctcaaggtg ataatacttt accaatcggg attaaaattc caccttttac ttaccaaaat
     5401 caatatgatg aattaatcaa aggcttatta caagcggtcg aaaatggcga aaatttacca
     5461 atttccttta ttacttcaac caatacgctt ggctcatcgc ttgtcttaaa tgctggcaaa
     5521 gcggtattaa attcagaagt gggaacgggc gtaggcggaa tggcaggaac ggcaatccat
     5581 gcactttctt tggggaatgt ttacacgatt cgccaaatgt tagatcaaga gcctaagctc
     5641 aaacatcttc aaattattgg tgtgggcggc gtgaatgata aagccggctt tgagcgaatg
     5701 aaatcggttg gggcagactt tgtggggtta gctaccgcat taggggtgaa aggaatcggc
     5761 gtttttaaag agattctgtc tgaataatga agaggagggc gtgcattgca cgcccgaata
     5821 aatgaaatta gcctaaccat ttctccacta attcattagt ggtttcttta tgtacccaca
     5881 tactgccaag ttccggcatt gggaatttgg tgtggttgta gccgacaaat tggcggaaat
     5941 caaacacggc gtgcgggtag ccgttgatca gtaaaaaggc ttgatagccg tcttcactcc
     6001 agcaaatttc taaacggcga ggttctttta atgaattttg ctcaatatcg ctgacccgaa
     6061 acacaaataa actatccact accggctgtt cggtttggtg taagtccatt gcataaaagt
     6121 agccgctttc ttcatcaatt tcaaacatca ctactaaatg ttcgtgctgg gttgaatgag
     6181 cgccattacg gtaaggttcg ttcagtagaa tatgagcagt taaaacagag tagagcatta
     6241 aattatcctt ataagtaaaa tgccaccgat ttctcagtgg catttaaata tttaatcagg
     6301 caattacgct tgacctttaa tctctttacg gccgttgaac ggagccggtg taccagcacg
     6361 ctctaacgct tcttcgatac ggattaactg gttgtatttc gctacacggt ctgaacggct
     6421 cattgaaccg gttttgattt gacctgctgc tgtacctact gctaaatctg caatggttgc
     6481 atcttcagtt tcgcctgaac ggtgagagat aactgcggtg taacctgcat ctttcgccat
     6541 tttgattgca gctaaagttt cagttaaaga accgatttgg ttgaatttga ttaagattga
     6601 gtttgcgata cctttctcaa taccgttttt caagattttg gtattggtta cgaataagtc
     6661 atcaccaact aattgtactt tgtcgcctaa tactttagtt tggtatgcga aaccttccca
     6721 gtcagattcg tcttgaccat cttcgataga cacgattggg tactctttgc ataagccttc
     6781 taagtagtga gtgaactctt gtgaagtaaa ggttttgcct tcgcctttca tatcgtacac
     6841 attgcgttct ttgtcgtaga attcagatga tgcacagtcc atcgctaaag ttacgtcttt
     6901 acctaacaca tagcctgctt tttctaccgc ttctttaatg cacgctaatg cgtctgcgtt
     6961 tgaagccaag tttggtgcga aaccaccttc gtcacctaca gcagtattta agcctttcgc
     7021 ttttaatact tttgcaagat tgtggaatac ttcagcaccg atacgaagag cttcttttaa
     7081 tgtagaagca cctaccggtt gaatcataaa ttcttgaatg tccacgttgt tgtcagcgtg
     7141 ttcaccacca ttgatgatgt tcatcattgg taatggcatt gagtaaacac ccggtgtgcc
     7201 gtttaattca gcaatgtatg cgtaaagcgg taagcctttt gaagcggctg ctgcttttgc
     7261 tgttgctaaa gaaaccgcta agatagcgtt tgcaccgaat tttgatttgt tttctgtgcc
     7321 atctaaatcg atcataattt ggtcgatttc agcttgtgca gaaccgtctt tgcctactaa
     7381 tgcgtttgca atttcgttat ttacggcaga aaccgctttt aatacacctt tacctaagaa
     7441 acgacctttg tcgccatcac gtaattctaa tgcttcacgt gaaccggtag aagcacctga
     7501 tggagcggct gctaagccta caaaaccacc ttctaagtga acttctgctt ctacagttgg
     7561 gttaccacga gagtcgatga tttcacgacc gattacttta acaattttag ccattgttga
     7621 tgtcctctaa tatacgagtt aatagaaaaa agaataggta tatagtaaag cgattttctg
     7681 cttttgtctt ggaaaaactg attgagtggc agaaaatttg ccactaattt gatctaagac
     7741 tatattcgcc tgagattata gcaacctttg caaaaaacgg caaaaatttg cccgcttgta
     7801 atgcttattt tttgactaaa aaagtcaagg tttcataatg tgctgtgtga gcaaacatat
     7861 caaacagctg cactttgtgt agttcgtaat ttgtcaaaaa tgtaaaatcg ttcgccattg
     7921 agacggcatt acaactggag taaatcaaat aaggtgagcc taattgattt aaaaattctg
     7981 ctaatagttt gccgatacca cgtcgaggtg ggttcacaat cactaaatcg ggtggagaac
     8041 cagcttcatt taaagcaaat tgtgcagaat ctaatgaggc aaattttacc tgctttaggc
     8101 cgagtttttc agccgatttg gtcgcacttg caatagctga ggctgaaatc tcaatgcctg
     8161 tcagctccac gttcgagttc ttcgcttgca aggcgttggc acaatgtaag ccgaaaccac
     8221 cgacaccgca gaacaagtcc cacaatttag caataggtaa atgctcaatc cagctttgtg
     8281 ctgtgtggta aagctggctt gctaccacag ggttagtttg gaaaaagccc tgagggcgaa
     8341 taaatagcgg tatgccgtta aaatgttctt ccagcacttt ttgttcggtg aggaaaatct
     8401 cagtttcgcc ttctaaaaga gcagcgtgtt ggggctgaat attcagacta actactgagt
     8461 tgggtggcag tttttccaac agttcgggca attcccgttc aaccaatgca cgctttgctt
     8521 cactacgaac cacaaaacgg agcataaatg attgattata ttggctttcg gtaatcagaa
     8581 tatattttag ctcacctttt ttcttactga tgttatacgg cactaaacca gctcgagcga
     8641 taaaatcttt caaaatcgga aaaagtctat gaaatgagga cgggtagagc agacagtcgg
     8701 ttaaatccac cccgctttga gcatcatttt gatcttttaa aatgcctaaa atcgggcgtt
     8761 ctacactgcc ggagaccacc attttcgctt tgttacgaaa ttgcgaaatt ggcgagccga
     8821 caagcggtaa gatttccgtt ttattttgca aaataaacgg caaaatcaac cgcttgagat
     8881 cggcctcttt ggcggctaat tgcgtgctgt atggcttttc aatccactgg cacgaagtac
     8941 agtcgccacg ctcaaaatgc gaacaatgca acaagctaga cattcgcttc cttccattcc
     9001 agccagttgt tttgtaaggc cgttaattca gcctgagttt gttggtagcg agagctgttt
     9061 tccagctctc gccaagtcac ggttttgcgt ttcattaaag aaaggcgacg ttctttttgt
     9121 gcttgagtcg gctgatattt cagcccgtct aacacttgca ccaggccttc aggctcgtta
     9181 caaaggagca ataaatcaca gccggcttgg attgcttttt cagaacgctc gacaaaattg
     9241 cccataaagc ccgcaccttt cattcccaaa tcgtcagaga atatcacacc gttaaagttc
     9301 agctgtttac gcaatacctc tttaagccaa tagcttgaac cgcttgccgg ctggctatca
     9361 cattgggtgt aaacgacgtg ggcaggcata atggcagaga gttttccttt ggcaataagc
     9421 tgtttgaacg gcagaatatc gtgattaaaa atccgctctt tcgggcgatc atcaaacggg
     9481 gtttctaaat gcgaatcggc taaaacgtgt ccatgaccgg ggaagtgttt gccggtggtc
     9541 gccataccga tttcacgcat tccgtcaata aaggcttcgg caacaggcaa aatcgctgct
     9601 ggctcttgcc cgaatgagcg atcgccaatc gctttgcatt cgtgtcctaa atccagtacc
     9661 ggagcgaaac tgaggtctat atcgagggca aacatttctg ctgccatcaa ccagccggtt
     9721 tctttcgcta attgcattgc ttgctctggc gttgtggcaa gttggttaaa agattgcatt
     9781 gccggcagtt tagtaaagcc ttcacgaaag cgttgcaccc gaccgccttc ttggtcgaca
     9841 gtaataagca acggtttttt cacccgctgg cgaatggatt tcactaatgc ctgaatttgt
     9901 gctttgtcgt agaaattacg ggtgaataaa attagcccgg aaactaaagg gtgttccagc
     9961 atttcgactt cttcttgagt cagttcttta tctttaatat caatcagtaa cataattttc
    10021 gttgagctat ttgcaaaaaa atgagcagat atgaccgctt gttgtgccta ttctacatta
    10081 aaaagccctt gatgttgatc aagggcgaat gattaagggc ataaatgaga cagagcaatt
    10141 atgcttgttc taatttagct tggttacgac gcatttttat gttgagcatt tcaaccacaa
    10201 ccgagaagcc cattgccgta taaaccgctg cttttggaat atgaatatca aaaccttcgc
    10261 cgactagtac aataccgatt aaaatcaaga acgctaaagc gagattttta atggttgggt
    10321 tgttatctac aaagtcccca atggttttcg ctgctaacat cattaccgca actgcaatga
    10381 taatcgcaat aaccattacc ggaatatcat ctgccattgc cacagcagta atgactgaat
    10441 caagggagaa aataatatca aaaattgcaa tttgaattaa caccataaaa aagctggctt
    10501 ttttaatgga ttcgcttttt tcttcgtgag attcggcaga aatggattct ttcagctcca
    10561 ttgcactttt aacaatcagg aataatccac cgatgagtag aatgagatct ctacctgaaa
    10621 ttggattgtt gccaatagtg aaaagaggct cgaccaaacg catcatccaa gcaagcgaga
    10681 gaagcaataa aatacgggtt cccattgcca gccccaagcc gataattcgg gcagattggc
    10741 gttgttcaat aggtaaacgg gaaacgagaa tactgatgac gatgatgtta tcaatgccta
    10801 atacgatttc aagcccggta agtgtaaaga gcgcaaccca agcttcagga tttgtgatcc
    10861 agtcaaacat aaaaatgtcc ttttaaaaat aagggattaa gtgatatttt ctatcaaata
    10921 gaattaaagc cgacatctta tagagtgtga ggacaaattt aaagggggca atgcaaaaag
    10981 ttttgtaaaa tttcggcggt aaaatgttta cgcaaataga aaccaagagg gagagattga
    11041 acaatctccc ctttagcgtt gatagcagag gtgatagagc ggttatttct acctttgggt
    11101 ctgagttgta acacttcgcc taaggttgca ttaattttat gcagttcacc taacacaatc
    11161 aactccatta attcctccca atcttgctgt aggcgggatt cttgtgtgat agtcggagac
    11221 cataaaatcg gctgaccgat atggcgttcg gcaaggggta tctttcgttc cccttcaacc
    11281 ggaatccaaa gcacacggga aagcttatgt ttaacgtggg aggtttccca aataataccg
    11341 ttgttttggg ttaaaggagc aaggctgacg aatgtggttt ccagcggtaa tcctttctga
    11401 ttgaccggaa tggttttcag ctcaatgcct aaatgtgcaa aatcctgttc ggctttactg
    11461 ccggctttcg cacctaaagc ggtttcaatc agagtaccga cccagccttt atctcgtttc
    11521 agatctgctg gtatcggaat atgaagcatc tcggcaattt cacctaaagt gaaccctgcc
    11581 agccattccg ctttgcttaa taatgcctgc tcggttggag agaatgtaga taattgcatt
    11641 gcacttagtt aattagacta aactggcaag cggtcaaatt taggcagaaa tttgcaaaaa
    11701 attactaaaa aataaccgct tgtgaggaaa ttacatccaa gcattactgc gaataacgcc
    11761 tactgcaatg ccttcaattt caatatattc ttgagcaggg tttactacaa tcggttttaa
    11821 ttcatcgttt tcagggtgta agtaaattaa ttcacctttc ttctctaagc gtttaacagt
    11881 cacttcatca tttacccgag caaccaccac ttgcccgtta cgagcggtat tggttttatg
    11941 aacggctaat aggtcaccgt ctaaaatacc gattttttcc attgaattgc catttacttt
    12001 gagtaaataa tcagcacttg gattaaacat cgcaccattc accgggtaat ggttttcaac
    12061 gtgttcaacc gctaaaatag gtgtgccagc agctactttg ccaatcaacg gtaagccttc
    12121 atcttcatcg ttggcagctt ggttttcgtc ttcgactaaa atgcgaattc cacgagatgt
    12181 acctgataac atttcaatat agcctttacg agcaagggct tttaagtgtt cttctgccgc
    12241 attaggtgat ttaaagccga tttcacgggc tatttctact cgggttggtg gcataccggt
    12301 ggtttcaata tggtttttga cgaaatcaaa aatttcttgc tgacgggcag ttagatgttt
    12361 acgcatttta agttaatcct cactgtcttt atatacagaa aatattgata gtatatacag
    12421 tatttttgat tttgtaacta ataatttcac gaaattccat aaaaattta
//
LOCUS       DANLXS010000064        12451 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.064, whole genome shotgun sequence.
ACCESSION   DANLXS010000064 DANLXS010000000
VERSION     DANLXS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12451
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.064"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..684
                     /gene="fkpA"
                     /locus_tag="PXD71_001302"
     CDS             <1..684
                     /gene="fkpA"
                     /locus_tag="PXD71_001302"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6291426.1"
                     /translation="TLFSTNVMAEEKADPKFVDDSSYAVGVLMGKNINGVIESQKDIF
                     TYNQDKILAGVQDTLKKTGKLTDEDLQKQLKSLDAYLSAKEAKINEEKTKATVAAGDK
                     FRADYKKQSGVKETASGLLYKIEKAGEGNSPKAEDTVKVHYKGTLTDGTVFDSSYDRG
                     EPIEFQLNQLIPGWIEAIPMLKKGGKMEIVVPPALAYGDRAAGKIPANSTLKFEIELL
                     DFKAADAKK"
     gene            781..1437
                     /locus_tag="PXD71_001303"
     CDS             781..1437
                     /locus_tag="PXD71_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6291427.1"
                     /translation="MTKKFTPFSDEDHAILASYFPMVDGIAALIGEHCEIVLHSLEFL
                     EHSAIYIANGHNTNRKIGSPITDRALKSLHHMATDSVSKPYFTKAKGGVLMKSITIAI
                     RNGKQHVIGLLCININLEVPTSQFLNSFIAPVDTASEVTFASSVEDLVSQTIQTTIDD
                     VMADRQISNNNKNRQIVISLFEKGIFDIKDSINQVAEKLDISRHTVYLYIRQIKQDND
                     "
     gene            1437..1814
                     /gene="tusD"
                     /locus_tag="PXD71_001304"
     CDS             1437..1814
                     /gene="tusD"
                     /locus_tag="PXD71_001304"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDL6291428.1"
                     /translation="MDYVLAIKSPVYGSQGAFLAYQVAEELLKANHTICQIFFFQEGV
                     SNANALVNPASDEVNLVEMWKKLAKLHRLPLHLCIAAAQRRGVVDEQISANATHNLAE
                     GFTLAGLGEFSQAVLKADRLLTF"
     gene            1816..2187
                     /gene="tusC"
                     /locus_tag="PXD71_001305"
     CDS             1816..2187
                     /gene="tusC"
                     /locus_tag="PXD71_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDL6291429.1"
                     /translation="MKKYKLAFLFTQPPFGSAVSREGLDALLAASAFCDEQEIAILFI
                     NDGVFNLIADQQPEKLLQKDHISTFKLLELYDLTECFICQESVSLRGLDNADWLLRML
                     QFKTHAEMIALLNQAEKVMTF"
     gene            2197..2472
                     /gene="tusB"
                     /locus_tag="PXD71_001306"
     CDS             2197..2472
                     /gene="tusB"
                     /locus_tag="PXD71_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDL6291430.1"
                     /translation="MLYTLSKANYDEETLNFFFSHLSENDAVVLWQDGVLQAVKSPQF
                     FAKLPRCYLLAQDVQARGLQEKLTAFQCISLSELVKLTAVYFPQVAL"
     gene            2588..3487
                     /locus_tag="PXD71_001307"
     CDS             2588..3487
                     /locus_tag="PXD71_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291431.1"
                     /translation="MVMAEENNTWVDKKHETVSTTLDRWANNINSWLGDADPDKPGTA
                     SLRVMLDSRWDKHDKFTYKPRVRGKIKLPILKKHLNVVFGDDELDNELTDKNHAGKLY
                     KNVNPNKRYNSREARDSNSSIGLRWSDGIKALGIETDLDAGIRSGSDIFGRLRISKDW
                     KITDQFSTRLEQIYRYGSESKHYLRTNFENKYTETDTTFIMNHTNFEYRHDVDEERSW
                     GNSLYRQHNFKPLTHISYGISMGGRLDKELSKFNYWGPFVSYRQPIWRNWLFIQPEIS
                     FYNDKDKDRKHFIQTFVRLEAIF"
     gene            3837..7862
                     /gene="rpoB"
                     /locus_tag="PXD71_001308"
     CDS             3837..7862
                     /gene="rpoB"
                     /locus_tag="PXD71_001308"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL6291432.1"
                     /translation="MAYSYSEKKRIRKSFGKRPQVLNVPYLLTIQLDSFEKFIEKDPE
                     GQQGLEAAFRSVFPIVSNNGATELQYVSYELGEPVFDVRECQIRGTTYAAPLRVKLRL
                     VSYDREAAAGTVKDIKEQNVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFARIDRRRKLPATIILRALGYTTE
                     EILNLFFEKIKFEIADNKLLMTLVPERLRGETASFDIAANGKVYVEMGRRITARHIRA
                     LEKDNVTQIEVPVEYIVGKVAAQDYVDLSSGEVICPANMEISMEMLANLSQAGYNQIE
                     VLFTNDLDHGPYISETLRVDPTYDRLSALVEIYRMMRPGEPPTKEAAEALFDNMFFSA
                     ERYDLSAVGRMKFNRSLEIPEGVGSGILSNDDIIGVMKKLIEIRNGRGEVDDIDHLGN
                     RRIRSVGEMAENQFRIGLVRVERAVRERLSLGDLDGVTPQDLINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHPTHYGRLCPIETPEG
                     PNIGLINSLSVYARTNNYGFLETPFRKVVNGQVTEEIEYLSAIEESNNIIAQANSNLD
                     ENFRFTDTYVTCRQQGESGLFKPEEVQYMDISTQQVVSVAAALIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMEKPIALDSGVAIVAKRGGTVQYVDASRIVVKVNEDE
                     TIPGEAGIDIYNLIKYTRSNQNTCINQVPCVNLGEPVARGEILADGPSTDLGELALGQ
                     NIRVAFMPWNGYNFEDSMLVSERVVQEDRFTTIHIQELSCVARDTKLGAEEITADIPN
                     VGESALSKLDESGIVYIGAEVKGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGTSGTVIDVQVFTRDGVEKDKRAKEIEEMQLCEAKKDLAEELEILEAGLF
                     TRVRNLLIEGGVKESALDGVAREKWLEQTLEDEAKQNQLEQLAEQHEELRKEFERKLE
                     IKRNKIIQGDDLAPGVLKVVKVYLAVKRQIQPGDKMAGRHGNKGVISKINPVEDMPYD
                     ENGQPVEIVLNPLGVPSRMNIGQILETHLGLAAHGIGDQIDKMIKQQQEVAKLREYMQ
                     KAYDLGHGSQVVDLSTFSDEEVMRLAQNLRKGLPLATPVFDGAHESEIKGLLELGGLP
                     TSGQITLYDGRTGEKFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGTHYMEPGIPE
                     SFNVITKEIRALAIDMELDEA"
     gene            8125..12408
                     /gene="rpoC"
                     /locus_tag="PXD71_001309"
     CDS             8125..12408
                     /gene="rpoC"
                     /locus_tag="PXD71_001309"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL6291433.1"
                     /translation="MKDLVKFLKAQTKSNEDFDVIKIGLASPDKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRER
                     MGHIELACPVAHIWFLKSLPSRIGLILDMPLRDIERVLYFESYVVTEPGMTDLEKNQL
                     LTEEQFMDAEERWGDEFEAKMGAEGIQALLRDLDLEHQCETLREELQETNSETKRKKI
                     TKRLKTLEAFLQSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLDLVAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSNKRPLKSLADMIKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFRPFIYSKLENRGIA
                     STIKAAKKMVEREDPIVWDILAEVIREHPILLNRAPTLHRLGIQAFEPLLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLTLEAQLEARALMMSTNNVLSPASGDPIIVPSQDVV
                     LGLYYMTREKVNGKGEGMYFLDPREAEKAYRTGQAELHSRVKVRVTEYEKNEAGELEA
                     KTSLVDTTIGRSILWMIAPKGMPFSLFNQTLGKKAISKLINESYRRLGLKESVVLADQ
                     IMYTGFAYAARSGASVGIDDMVIPEQKYAIIEAAEAEVAEIQEQFNGGLVTAGERYNK
                     VIDIWAAANERVAKAMMENLSTEEVINREGNPEKQASFNSIFMMADSGARGSAAQIRQ
                     LAGMRGLMARPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLT
                     RRLVDVAQDLVIIEDDCGTHEGIVMTPLIEGGDVKEALRDRVLGRVVAEDVLKPGTDE
                     VLIPRNTLLDEKWCDVVDRESVDVIKVRSVVTCDTDFGVCAKCYGRDLARGHLINQGE
                     AVGVIAAQSIGEPGTQLTMRTFHIGGAASAAAKESSIQVKNAGTIKLTNAKFVTNKEG
                     KIVLTSRNTELTVIDTFGRTKENYKVPYGAVLSKHDGAEVAVGEVVANWDPHTMPVIS
                     EVSGRIQFSDIVDGLTVTRQTDELTGLSSIVVQDVGERATAGKDLRPALRLVDSNGKD
                     ILIPGTDVAAQYFLPGKAIVTLDDGAEIEVGEALARIPQESVGTKDITGGLPRVADLF
                     EARKPKEPAILAEISGIVSFGKETKGKRRLVITPAEGEAFEEMIPKWRQLNVFEGEMV
                     QRGDVISDGAETPHDILRLRGVHAVTDYIVNEVQEVYRLQGVKINDKHIEVIVRQMLR
                     KAVITNAYDSEFLEGEQVEVARVKIANRKRAEEGKPLVEFERELLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKQDELRGLKENVIVGRLIPAGTGFAYHQARAKKRAQLEA
                     GIAVVPEFEAPAAKANSFVTDADIEAEFEFVADDATQSLAALLNAGEEE"
BASE COUNT         3755 a         2415 c         2873 g         3408 t
ORIGIN      
        1 acacttttta gtacaaatgt gatggcagaa gaaaaagcag atcctaaatt tgtggatgat
       61 tcctcttatg ccgtgggtgt attaatgggt aaaaatatta atggtgtgat tgagtcacaa
      121 aaggatattt ttacctataa ccaggataaa atcttagccg gtgttcagga caccttaaag
      181 aaaaccggta aattaaccga tgaagatttg caaaagcagt taaaatcgtt agatgcctat
      241 ttatctgcga aagaagcaaa gattaacgaa gaaaaaacca aagcaacggt agctgcaggc
      301 gataaattcc gtgctgatta taaaaaacag tctggcgtaa aagaaacggc ttcaggcctg
      361 ttatataaaa ttgaaaaagc aggtgaaggc aattcaccaa aagcggaaga tactgtgaag
      421 gtgcattaca aaggcacttt gactgatggc acggtatttg acagctctta cgaccgtggt
      481 gagccgattg aatttcagtt aaatcaatta attccgggtt ggattgaagc gattcctatg
      541 ttgaaaaaag gcggtaagat ggaaattgta gtgccacctg cattagctta tggtgatcgt
      601 gccgcaggca aaattccagc aaattcaacc ttgaaatttg aaattgaatt attagatttt
      661 aaagcagcag atgctaagaa ataattcgtt attccttaat tattgaagat acgtaagcgg
      721 ttagatttat tataatattt gcaaaaaatg tgataaaact aaccgcttgt aatttgtatt
      781 atgaccaaaa aatttacgcc tttttctgac gaagatcacg ccattcttgc ttcttacttt
      841 ccgatggtag atgggattgc ggcactgatt ggtgagcatt gcgaaattgt attgcattcg
      901 ttagagtttc tggaacattc ggcaatttat attgctaacg gacacaatac taaccgtaaa
      961 atcggctctc cgattactga ccgtgcattg aaatcgctgc atcatatggc aacggatagc
     1021 gtctccaaac cttattttac caaggcaaaa ggtggcgttt taatgaagtc tatcactatt
     1081 gcgattcgta atggtaaaca gcacgttatc gggttactgt gcattaatat taatttggaa
     1141 gtgcctacct cacagtttct caattctttt attgcccctg tagataccgc ttcagaagtt
     1201 acttttgcca gttcagtaga agatttggtt tcacaaacga ttcaaacgac cattgatgat
     1261 gtgatggcag atcgtcaaat ttctaacaat aataaaaatc gtcaaattgt gatttcgtta
     1321 tttgagaaag ggatttttga tattaaagat tccattaacc aagtagcgga gaagctggat
     1381 atttcacgcc atactgtgta tctctatatt cgtcaaatta agcaggacaa tgattaatgg
     1441 attatgtatt agcgataaaa tcaccggttt atggttcaca aggggcattt ttagcctacc
     1501 aagtggcgga agaattatta aaagccaatc atacaatttg ccaaatattc ttttttcaag
     1561 aaggcgtgag caatgctaat gccttagtga atccagcaag cgatgaagtg aatttagtcg
     1621 agatgtggaa aaaattggct aaattgcacc gcttgcctct gcatttgtgc attgctgctg
     1681 cccagcgtag aggcgtggtg gatgagcaaa tctcagctaa tgcaacacat aatttagccg
     1741 aaggatttac cctagccggg ttaggcgaat ttagccaagc agtattaaaa gcggatcgat
     1801 tactgacttt ttaagatgaa gaaatataaa cttgcctttt tatttaccca gccacctttt
     1861 ggttcggcag ttagccgtga ggggctagat gccttgcttg cagctagtgc tttttgtgac
     1921 gagcaagaga tagctatcct ctttatcaat gatggcgtat ttaatctcat tgccgatcaa
     1981 cagccggaaa aactcttaca aaaagatcat atttccacct tcaaattatt ggagttgtat
     2041 gacttaactg aatgttttat ttgccaagaa agtgtaagtt tacgagggct ggataatgca
     2101 gattggttac tgcgaatgct tcaatttaaa actcacgctg agatgatcgc attgctcaat
     2161 caagctgaaa aagtaatgac gttttaagga atatttatgc tttataccct ttccaaagcg
     2221 aattatgatg aagaaacctt aaacttcttt ttctcacatc ttagtgagaa cgatgcggtt
     2281 gttctgtggc aagatggcgt gctacaagcg gtcaaaagtc cgcaattttt tgcaaaattg
     2341 ccccgttgct atttattggc acaagatgtg caagctcgag gattgcaaga gaagctcact
     2401 gcatttcagt gcatttcatt atccgaatta gtcaaattaa cagcggttta ttttccccag
     2461 gttgcactgt aacctagaga ggatattttt tgtaatacca aataatttag attatgatgt
     2521 gattcctaaa aaggagaata ttatgaaaac aaaatgtctt attccgctac tagcacttac
     2581 acctgttatg gtgatggcag aagaaaataa cacttgggta gacaagaaac atgaaacagt
     2641 aagcactacc ttagatcgtt gggcaaacaa tattaacagt tggctaggtg atgcagaccc
     2701 agataaaccg ggaacggctt ctttacgggt aatgttagat agccgttggg ataaacacga
     2761 taaatttact tataagcccc gtgttcgtgg taaaatcaaa ctccctatct tgaaaaagca
     2821 tctaaatgtc gtatttggtg atgacgaact ggataatgag cttaccgata aaaatcatgc
     2881 tggaaaattg tataaaaacg tcaatccaaa taaacgctat aactctcgtg aagcgagaga
     2941 ttcgaactct tctatcggtt tacgttggtc tgatggcatt aaggctttag ggattgagac
     3001 cgatttagat gctggtattc gttcgggaag cgatattttc gggcgtttac gcatcagtaa
     3061 agattggaaa atcaccgacc agttcagtac ccgcctagag caaatttatc gttacggttc
     3121 agagagcaag cactatttac gtactaactt tgaaaataaa tataccgaaa cggataccac
     3181 ctttattatg aaccacacca attttgaata tcgtcatgat gtagatgaag aaagaagttg
     3241 ggggaatagt ttatatcgtc aacataactt caaaccactc acgcacatca gctatggtat
     3301 cagtatgggg gggcgtttag ataaagagtt atcaaaattt aactattggg gtccatttgt
     3361 ctcctatcgt caaccaatct ggcgcaactg gctctttatc cagcctgaaa tcagttttta
     3421 taacgacaaa gataaagatc gtaagcactt tattcagaca tttgtacgtt tagaggctat
     3481 cttctagcta ttaagcctca agtagaatct tggggcttgt ttttgcaagc ggtcaatttc
     3541 ttgccaaaat ttacaaaatc cgctataatt cctaaccatt tttagcaaaa attggctgac
     3601 ggcttgaccg gtcagccttt tgtgcttgaa cgcaagggct gattttgtca ttttaatcag
     3661 caaaatttgc aaatttcctg cccaatttga ccgcttgcag tagcgatttc tgactgcttg
     3721 attggcgatt ggaaaatgac atctcgtaca gtttttataa tgccacttcc aaccgagtct
     3781 ttcgtgtcgg attgtgtaat ctcaaccaaa atatatttca gaggctatct taaaaaatgg
     3841 catattcata ttccgagaaa aagcgtatcc gtaagagctt tggcaaacgc ccacaagttc
     3901 tgaacgtacc ttatctatta acaattcagc ttgattcttt tgaaaaattt atcgaaaaag
     3961 atcctgaagg gcagcaaggt ttagaagcgg cattccgttc tgttttcccg attgtgagca
     4021 acaatggtgc aacagaatta caatacgttt cttacgaatt aggcgagccg gtgtttgacg
     4081 tgcgtgagtg ccaaattcgt ggtacaacct acgctgcacc attacgcgtg aaattgcgtt
     4141 tagtctctta cgatcgtgaa gccgcagccg gtacggtaaa agacattaaa gagcaaaacg
     4201 tgtatatggg cgaaatccca ttaatgaccg ataacggtac tttcgtgatt aacggcaccg
     4261 agcgtgttat cgtttctcaa ttacaccgta gcccaggtgt gttcttcgat tctgacaaag
     4321 gtaaaaccca ctcttcgggt aaagtgcttt ataacgcacg cattatccct taccgtggtt
     4381 cttggttaga tttcgagttt gatccgaaag ataacttatt tgcccgtatc gaccgccgtc
     4441 gtaaattgcc ggcaaccatt attctacgtg cattaggcta caccaccgaa gaaatcttaa
     4501 atttattctt cgagaaaatt aaatttgaaa ttgccgataa taaattattg atgacgttag
     4561 tgcctgagcg tttaagaggc gagactgcat catttgatat tgcagcaaac ggcaaagtat
     4621 atgttgaaat gggtcgccgt attaccgctc gtcatatccg tgcgttagaa aaagacaatg
     4681 tgacccaaat tgaagtgccg gtggaatata ttgtcggcaa agtagcggct caagattacg
     4741 ttgatttatc ttcaggtgaa gtgatttgcc cggcgaatat ggaaatctcg atggagatgt
     4801 tggcgaattt atcacaagcc ggttacaacc agatcgaagt attatttacc aacgatttag
     4861 accacggtcc atatatttct gaaaccttac gtgttgatcc aacttacgac cgtttaagtg
     4921 cgttggttga aatctatcgt atgatgcgtc cgggcgaacc accaacgaaa gaagcggcag
     4981 aagcattatt cgataatatg ttcttctcgg cagagcgtta tgatttatcg gcagtaggtc
     5041 gtatgaaatt caatcgctcg ttagaaattc cggaaggcgt aggcagcggt attttaagca
     5101 acgatgacat catcggtgtg atgaaaaaat taatcgaaat ccgtaacggt cgtggtgaag
     5161 tggatgatat cgaccactta ggtaaccgtc gtatccgcag cgttggtgaa atggcagaaa
     5221 accaattccg cattggtttg gtgcgtgtag aacgtgcggt gcgtgagcgt ttatctttag
     5281 gcgatttaga tggtgtaaca ccacaagatt taatcaacgc taaaccgatt tcagcagcag
     5341 tgaaagagtt ctttggttca tcacaacttt cgcaatttat ggaccaaaac aacccgcttt
     5401 cagaggtaac acataaacgc cgtatctcgg cattaggccc gggtggttta acccgtgagc
     5461 gtgccggctt tgaagtgcgt gacgtacacc cgactcacta tggtcgttta tgtccaatcg
     5521 aaactcctga aggcccgaat atcggtttga tcaactcact atcggtgtat gctcgtacta
     5581 ataactacgg tttcttagag actccattcc gtaaagtggt gaatggtcaa gtaaccgaag
     5641 agatcgagta tttatctgcg attgaagaaa gcaacaatat cattgcacag gcgaactcaa
     5701 acttagatga gaacttccgt tttaccgata cttatgtaac ttgccgtcag caaggcgaat
     5761 cgggcttatt caaaccggaa gaagttcaat atatggacat ctcaacccaa caagtggttt
     5821 ctgtggctgc ggcgttaatt ccgttccttg agcacgacga tgcgaaccgt gcgttaatgg
     5881 gtgcgaacat gcaacgtcag gcagttccaa ccttgcgtgc ggacaaacca ttagtcggta
     5941 ccggtatgga aaaaccaatt gctcttgact caggtgtagc gattgtcgca aaacgtggtg
     6001 gtacggtgca gtatgttgat gcgtcacgca ttgtagtgaa agtaaatgaa gatgaaacca
     6061 ttccgggcga agcgggtatc gatatttata acttaatcaa atacacccgt tcaaaccaaa
     6121 acacctgtat caaccaagta ccttgtgtga acttgggtga gccggttgct cgtggtgaaa
     6181 tcttagcgga cggtccttca acagacttag gtgaattggc attaggtcaa aatatccgtg
     6241 tggcatttat gccttggaac ggttataact tcgaagactc aatgttagtt tctgagcgtg
     6301 tggtacaaga agatcgcttt accactatcc acattcaaga attatcttgt gtggcgcgtg
     6361 ataccaaatt aggtgcagaa gagatcaccg ctgatattcc aaacgtgggt gaatctgcat
     6421 taagcaaatt agatgaatca ggtatcgttt acatcggtgc agaagtgaaa ggtggcgaca
     6481 tcttagtagg taaagtaact cctaaaggtg aaacccaatt aacaccggaa gaaaaactat
     6541 tgcgtgcgat cttcggtgaa aaagcctctg atgtgaaaga ttcatcattg cgtgttccaa
     6601 acggtacttc aggtacggtt atcgatgttc aagtatttac ccgtgatggc gtagagaaag
     6661 ataaacgtgc gaaagaaatc gaagagatgc aactgtgtga agcgaagaaa gacttagcgg
     6721 aagagttgga aatcttagaa gccggtttat ttactcgtgt tcgcaaccta ttaattgaag
     6781 gcggcgtaaa agaatctgca ttagatggcg ttgctcgtga aaaatggtta gaacaaaccc
     6841 ttgaagacga agcgaagcaa aatcagttag aacagttagc ggaacagcac gaagagttac
     6901 gcaaagaatt tgaacgtaaa cttgagatca aacgcaacaa aattatccaa ggcgacgatt
     6961 tagcaccggg tgtgttaaaa gtagtgaaag tttaccttgc ggtgaaacgc caaatccagc
     7021 cgggtgataa gatggcgggt cgtcacggta acaaaggggt tatctcgaaa atcaaccctg
     7081 tggaagatat gccatacgat gaaaacggtc agccggttga aatcgtattg aacccgctgg
     7141 gcgtaccgtc tcgtatgaac atcggtcaga tcttagaaac ccacttaggc ttagcggctc
     7201 atggtatcgg tgatcagatt gataagatga tcaaacagca acaagaagtg gctaaattac
     7261 gtgaatatat gcaaaaagcc tacgatttag gtcacggctc acaagtcgtt gatttaagca
     7321 ctttctctga tgaagaagtg atgcgtttag cgcaaaactt acgcaaaggc ttaccgcttg
     7381 ctactccggt attcgatggt gcacacgaaa gcgaaatcaa aggcttatta gagttaggcg
     7441 gcttaccgac ttcaggtcag atcacacttt atgatggtcg taccggtgag aaatttgagc
     7501 gtccggtaac cgttggttat atgtatatgc tcaaattgaa ccacttggtt gatgataaaa
     7561 tgcacgctcg ttcaaccggt tcttatagcc ttgttactca acaaccattg ggtggtaaag
     7621 ctcaattcgg tggtcagcgt ttcggtgaga tggaggtttg ggcattagaa gcatacggtg
     7681 cggcttatac tcttcaagaa atgcttacgg tgaaatcgga cgatgtgaac ggtcgtacga
     7741 agatgtataa aaacattgta gatggtacac attatatgga gccgggtatc cctgaatcct
     7801 ttaacgtaat taccaaagag atccgtgcat tagcaattga tatggagctg gacgaagcgt
     7861 aatggtttca ctctccccct tgatggggga gagacaggca aaggatttgt tcagaatcct
     7921 ttggcagaga gagggtgatg caagcggtgt aaatttgcta aatttttgca aaaatcgacc
     7981 gcttaccctc tccctgattt tgcttctgaa cgaggcaaaa tctatccctc tcccacaagg
     8041 ggagagggtt taaaatcgca accaaattcg tagagtggat attctttcca ccaaacaaaa
     8101 caacctcaaa ctaggggcaa aaaagtgaaa gacttagtga agtttttaaa agcacaaaca
     8161 aaatcgaacg aagattttga tgtgattaaa attggtttag catcaccgga caagatccgt
     8221 tcttggtctt ttggtgaagt gaaaaaaccg gaaaccatta actatcgtac cttcaaaccg
     8281 gagcgtgatg gtttattctg tgcacgtatt ttcgggccgg taaaagatta cgagtgcttg
     8341 tgtgggaaat acaaacgttt aaaacaccgt ggcgtaattt gtgaaaaatg tggtgttgaa
     8401 gtaacccaaa ctaaagtacg tcgtgagcgt atggggcaca ttgaattagc ttgccctgtc
     8461 gcacacatct ggttcttaaa atcactaccg tcccgtatcg gtttaatctt agatatgccg
     8521 ttgcgtgata ttgaacgtgt gctttatttt gaatcttatg tagttactga accgggaatg
     8581 accgatttag agaaaaatca gttattaacc gaagaacaat ttatggacgc tgaagagcgt
     8641 tggggtgatg aatttgaagc gaaaatgggt gcagaaggta tccaagcatt acttcgtgat
     8701 ttagatttag aacaccaatg cgaaaccttg cgtgaagagt tacaagaaac caattcagaa
     8761 accaagcgta aaaaaatcac taagcgttta aaaaccttag aagcattctt acaatccggc
     8821 aacaaaccgg aatggatggt gatgacagta ttaccggtac ttccaccgga tttacgtccg
     8881 ttagtaccgc ttgacggtgg acgttttgcg acttctgact taaacgattt ataccgccgt
     8941 gtaattaacc gtaacaaccg tttaaaacgc ttgttagatt tagttgcacc ggacatcatc
     9001 gtgcgtaacg aaaaacgtat gttacaagag tcggttgatg cgttattaga caacggtcgc
     9061 cgtggtcgtg cgattaccgg ttcgaacaaa cgtccgttaa aatctttggc agatatgatc
     9121 aaaggtaaac aaggtcgttt ccgtcaaaac ttattaggta aacgtgtaga ttactcaggt
     9181 cgttcggtaa ttaccgtagg cccatactta cgcttacacc aatgtggttt accgaagaaa
     9241 atggcgttgg aattattccg tccgtttatc tactcaaaat tagaaaaccg tggcattgct
     9301 tcaactatta aagccgcgaa gaaaatggta gagcgtgaag atcctatcgt atgggatatt
     9361 cttgccgaag tgattcgtga acacccgatt ctacttaacc gtgcaccaac acttcaccgt
     9421 ttaggtatcc aagcgtttga gccgttattg attgaaggta aagcgatcca gttacaccca
     9481 ctcgtgtgtg cggcgttcaa tgcggacttc gacggtgacc aaatggcggt tcacgttcca
     9541 ttaacccttg aagcacagtt agaagctcgt gcgttaatga tgtcaaccaa caacgtactt
     9601 tcaccggcaa gtggtgatcc gattatcgta ccgtctcagg acgttgtact cggtctttac
     9661 tatatgaccc gtgagaaagt aaacggtaaa ggtgagggaa tgtacttcct tgaccctcgt
     9721 gaagcggaaa aagcataccg taccggtcag gctgaattac attctcgtgt taaagtgcgt
     9781 gttactgaat atgagaaaaa cgaagcaggc gaattagaag caaaaacttc gttagtagat
     9841 accaccattg gtcgttcaat cttatggatg attgcaccaa aaggtatgcc gtttagcttg
     9901 ttcaaccaaa ccttaggcaa aaaagcgatc tcgaaattaa ttaacgagag ctatcgccgt
     9961 ttaggtttaa aagaatctgt ggttttagct gaccaaatta tgtacaccgg ctttgcttat
    10021 gctgctcgtt ctggtgcctc agtaggtatc gacgatatgg taattccgga gcaaaaatac
    10081 gcaatcattg aagcggctga agcggaagtc gctgagatcc aagagcaatt caacggcggt
    10141 ttagtaacag ccggtgagcg ttataacaaa gtgatcgata tttgggctgc tgcaaacgaa
    10201 cgtgttgcga aagcaatgat ggaaaacctt tcaacggaag aagtaatcaa ccgtgaaggt
    10261 aatccggaaa aacaagcctc atttaacagt atctttatga tggcggactc cggtgctcgt
    10321 ggttctgctg cacagattcg tcagttagcg ggtatgcgtg gcttgatggc tcgtccggac
    10381 ggttcgatta tcgaaacccc aattactgca aacttccgtg aaggtttgaa cgtattacag
    10441 tactttattt caacccacgg tgcacgtaaa ggtcttgccg atacggcatt aaaaacagcg
    10501 aactccggtt acttaacccg tcgtttagtt gatgtggcac aagatttagt tatcattgaa
    10561 gatgactgtg gtacgcacga aggtatcgtg atgacaccac ttatcgaagg tggtgatgtg
    10621 aaagaagccc tgcgtgatcg tgtattaggt cgtgtggtgg cagaagatgt gttaaaaccg
    10681 ggtacagatg aagtattaat tccacgcaac accttattag atgaaaaatg gtgtgatgtg
    10741 gtggatcgtg aatctgtgga tgtgatcaaa gtacgttcgg tagtaacttg tgatacagac
    10801 ttcggtgtgt gtgctaaatg ttacggacgt gacttggctc gtggtcactt aatcaaccaa
    10861 ggtgaagcgg tcggtgttat tgcggcacaa tctatcggtg agccgggtac acagttaacg
    10921 atgcgtacgt tccatatcgg tggtgcggct tctgcggcag caaaagaatc tagcatccaa
    10981 gtgaaaaacg caggtacgat taaattaacc aacgctaaat ttgtaaccaa caaagaaggc
    11041 aaaatcgtat taacttctcg taatactgaa ttaactgtca tcgacacctt cggtcgtacc
    11101 aaagaaaact ataaagtgcc ttatggtgcg gtgctttcta aacacgacgg tgcagaagtg
    11161 gctgtgggtg aagtggttgc gaactgggat ccgcacacaa tgccggtaat ctcagaggta
    11221 tcaggtcgta tccaatttag cgacatcgta gatggcttaa ccgttactcg ccaaaccgat
    11281 gaattaaccg gtttatcatc tatcgtggtg caagatgtgg gtgaacgtgc aaccgcaggt
    11341 aaagatttac gtccggcatt acgcttagtt gatagcaacg gcaaagacat cttaattccg
    11401 ggtacagacg ttgcagcaca atacttctta ccgggtaaag caatcgtaac cttagacgat
    11461 ggtgcagaaa ttgaagtcgg tgaagcctta gcccgtattc cacaagaatc tgtggggacg
    11521 aaagatatta ccggtggtct tccacgcgta gcagacttat tcgaagcccg taaaccgaaa
    11581 gagccggcaa tcttggcgga aatttcaggt attgtgtcgt tcggtaaaga aaccaaaggt
    11641 aaacgccgct tggtaattac tccggcggaa ggcgaagcgt ttgaagagat gattccaaaa
    11701 tggcgtcagc tcaacgtatt tgaaggcgag atggtacaac gtggtgatgt gatatcagac
    11761 ggtgcagaaa ctccgcacga catcttacgc ttacgtggcg ttcacgccgt taccgactac
    11821 atcgtaaacg aagtgcaaga agtttaccgc ttacaagggg taaaaattaa cgacaagcac
    11881 atcgaagtga ttgttcgcca aatgttgcgt aaagctgtga tcaccaacgc ttacgactct
    11941 gaattcttag agggcgaaca agtggaagtg gctcgtgtga aaattgccaa ccgcaaacgt
    12001 gctgaagaag gcaaaccgtt ggtagaattt gaacgtgaat tgcttggtat taccaaagca
    12061 tctcttgcaa cagaaagctt tatttctgca gcctcgttcc aagaaaccac tcgagtgctt
    12121 accgaagccg cagtggcagg taaacaagat gaattgcgtg gcttaaaaga gaacgtaatc
    12181 gttggtcgct taatcccggc aggtaccggt tttgcttacc accaagcacg agctaaaaaa
    12241 cgtgcccaac tggaagcagg aattgcagtt gtccctgaat ttgaagcacc ggcagctaaa
    12301 gccaattcat tcgttaccga tgcagacatc gaagctgaat ttgaatttgt tgcagacgat
    12361 gcaacccaaa gtttagcagc gttgttaaat gcgggtgagg aagagtaata gctcgtaggg
    12421 tatgataaat taaaaaatct cctagaatat a
//
LOCUS       DANLXS010000065        12292 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.065, whole genome shotgun sequence.
ACCESSION   DANLXS010000065 DANLXS010000000
VERSION     DANLXS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12292
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.065"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            34..693
                     /locus_tag="PXD71_001310"
     CDS             34..693
                     /locus_tag="PXD71_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6291434.1"
                     /translation="MFNISKQQVLDAFHFRSACRSYDPNKKINKEDMDYILELGRLSP
                     SSVGSEPWKFLVLQNPETRQKIAPVSWGIKHPMEEMSHLVVILTKKNVRYDSVYMRDM
                     LAKRGLTYEQIEASLVRYKSFQEEDMKLLSERELFDWSTKQTYIALGNMMTGAALIGI
                     DSCPIEGFHYDTVNQILSDEGLFDLNEYGVSCMITFGYRNKEIKKKSRKPTEEVIAWI
                     E"
     gene            complement(753..2078)
                     /locus_tag="PXD71_001311"
     CDS             complement(753..2078)
                     /locus_tag="PXD71_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL6291435.1"
                     /translation="MLIFNLIVFAAILFLLAKIYKSTQKLGQTVFIGLFLGLASGAFL
                     QTFYEKATVDATLDWINLIGNGYVRLLQMIVMPLVFVSILSAITRLKEAGSLGKISFS
                     VLSVLLVTTAIAAAIGIALVYLFDLSAEGLVAGSRELAAQEKVVGRVEQVSNLSVPSM
                     LLSFIPKNPFLELTGANPTSIINTVIFSALLGIAALSLGKEDKALGERIAQGVETLNK
                     LVMRLVRFVIRLTPYGVFALMIKMAATSQWSDIVNLGSFIVASYIAIGLMFIVHGILL
                     FVAKINPLDYYKKVLPTLSFAFTSRSSAATIPLNIETQTNKLGNNSVIANFSATFGAT
                     IGQNGCAGIYPAMLAVMVAPTVGMDPFSLNYILTLILVVAISSFGIAGVGGGATFAAI
                     VVLSTLNLPIELVGLLISVEPIIDMARTALNVNGAMVAGTLTNKWVKAE"
     gene            complement(2179..2931)
                     /locus_tag="PXD71_001312"
     CDS             complement(2179..2931)
                     /locus_tag="PXD71_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon-containing protein"
                     /protein_id="HDL6291436.1"
                     /translation="MYQAIVHFSYHNFEQDPVTAISQILNQWLYNGQVIGREMPITFH
                     QNEFQARVCVPTQESLLPQYNSDEVNEALQQAVEIGVIFASFELVGRDYQGEESSHNR
                     SPAFQILYTTHLDTCSPLYDGEQFAPIPLYSLQDQSLSEAILEWQQNWQACDLLQMNG
                     NLLECHALVQISDETSDLAMIGRDLCQQIEQKTHIPTFYYLYRLGRDESEYQRKCPCC
                     GGEWKLTEPLHDIFHFRCEPCRLISNLSWELL"
     gene            complement(2931..3836)
                     /locus_tag="PXD71_001313"
     CDS             complement(2931..3836)
                     /locus_tag="PXD71_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL6291437.1"
                     /translation="MNEKYSKLVKNAANLAIIVAILLIVIKAFAWWKTGSISILAAMT
                     DSVVDLFASLTNILVLRFALQPADENHAFGHGKAESLAALAQSAFISGSAVFLLLQGF
                     HKLTNPELIAQSSIGVIVSIVSIVITALLVFYQRYVVTLTKSPAIEADSLHYQTDLLM
                     NAAILVAMLLNSAGFVYADAVFAIGIALYIAFNAVKMFWCAVQTLLDQALPDEEIERI
                     KEIASQHPNILGIHDILTRRAGAVRFIQLHLELDDHLTLLVAHEITDSLEQKLLAAFP
                     MSHVIIHQEPTTVVKQEFQDMAENI"
     gene            complement(3845..4408)
                     /locus_tag="PXD71_001314"
     CDS             complement(3845..4408)
                     /locus_tag="PXD71_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HDL6291438.1"
                     /translation="MSAIKKGITLVALSLAGCVSQSPQFAVPDAIHFGQDTFVKVTHS
                     QIDEMQHLLYLPEKSEKNPENWQKGILFFLDKNSKNQTLEQRAAFRQASFAKKPELEA
                     KVEIQQNELHSSVIYPPIERFNNVMLEVTRGRNLACGYGQIQFADKREVGKTVAKKSP
                     NLTAYSQEIARLSLELNQLGWQVQCSK"
     gene            complement(4408..5184)
                     /gene="yaaA"
                     /locus_tag="PXD71_001315"
     CDS             complement(4408..5184)
                     /gene="yaaA"
                     /locus_tag="PXD71_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDL6291439.1"
                     /translation="MLAIISPAKTLDFKTNIDGFAFSQPNLTACSQELIDICKQLSPA
                     ELGSLMSISDKLAALNVARFAEWQLEHNEQNAKAALFAFKGDVYTGLEAETLSKAQVE
                     YAQQHLRMLSGLYGLLKPLDLMQPYRLEMGTKLANPKGKDLYAFWGNIITENLQQAID
                     EQGDDILVNLASDEYYGAVKPQNLNATIIKPVFLDEKNGKYKVISFYAKKARGMMVRF
                     ILDTQPTSIEQLKQFNYGGYWFDADSSSESELVFKREEQA"
     gene            complement(5373..7093)
                     /gene="ltrA"
                     /locus_tag="PXD71_001316"
                     /pseudo
     CDS             complement(5373..7093)
                     /gene="ltrA"
                     /locus_tag="PXD71_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341372.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(8775..9170)
                     /gene="rpsI"
                     /locus_tag="PXD71_001317"
     CDS             complement(8775..9170)
                     /gene="rpsI"
                     /locus_tag="PXD71_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL6291440.1"
                     /translation="MTAANQNYGTGRRKSSSARVFIKPGSGNITINQRSLEVYFGRET
                     ARMIVRQPLELVELTDKLDLYITVKGGGISGQAGAIRHGITRALMEYDETLRPALRAA
                     GFVTRDARRVERKKVGLHKARRRPQYSKR"
     gene            complement(9187..9615)
                     /gene="rplM"
                     /locus_tag="PXD71_001318"
     CDS             complement(9187..9615)
                     /gene="rplM"
                     /locus_tag="PXD71_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL6291441.1"
                     /translation="MKTFVAKPETVQRDWYVVDATGKTLGRLATELARRLRGKHKPEY
                     TPHVDTGDYIIVINADKVAVTGKKQTDKIYYWHTGYVGGIKDATFKEMIARRPEAVIE
                     IAVKGMLPKGPLGREMFRKLKVYAGSEHNHQAQQPQVLDI"
     gene            9914..10309
                     /locus_tag="PXD71_001319"
     CDS             9914..10309
                     /locus_tag="PXD71_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcB family protein"
                     /protein_id="HDL6291442.1"
                     /translation="MEQWTTDVWAAIMAAFIVGAVIGCTLVRVFNSSVRKQHELEKEL
                     KQTKTQVDEQKQKLEQHFEQSAQLLSSLASDYKKLYTHLAQSSEKLLPEASKNITFFQ
                     QTKLPIPAEKTEEDQPRDYSEGSSGLLKS"
     gene            complement(10470..12041)
                     /gene="guaA"
                     /locus_tag="PXD71_001320"
     CDS             complement(10470..12041)
                     /gene="guaA"
                     /locus_tag="PXD71_001320"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL6291443.1"
                     /translation="MTNIHHHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQ
                     IREFNPTGIILSGGPESTTEENSPRAPEYVFNAGLPVLGICYGMQTMAMQLGGLTETS
                     DHREFGYASVALKSADQLFANLNDDLTACEPKLDVWMSHGDKVTKLPPHFTITGVTPT
                     CPIAAMSDESRRFYGVQFHPEVTHTKQGLALLKNFVVGICGCETNWTAENIIEDAVKR
                     IREQVGDDEVILGLSGGVDSSVTALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFG
                     NKFGLNIVRVNAEDRFLEALKGLDEPEAKRKTIGKVFVDVFDDESKKLSSVKWLAQGT
                     IYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLP
                     AEMLNRHPFPGPGLGVRVLGEIKKEYCDLLRKADAIFIEELHKAGWYYKVSQAFTVFL
                     PVKSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDLLGKISNRIINEVNGISRV
                     VYDVSGKPPATIEWE"
     gene            complement(12058..>12292)
                     /locus_tag="PXD71_001321"
     CDS             complement(12058..>12292)
                     /locus_tag="PXD71_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291444.1"
                     /translation="TASPLLKLDTERNINLYFQAIHKCTPDKIHTKIINIPSEGNAKE
                     LWTATGCNKTQSFIITYRNDGVGGTFINMTTTP"
BASE COUNT         3496 a         2761 c         2331 g         3704 t
ORIGIN      
        1 aatctactta tttcacttat aaaaggaaaa acgatgttta atattagcaa acagcaagtt
       61 ttagacgctt ttcacttccg ttcggcctgt cgcagttacg atccaaacaa aaaaatcaac
      121 aaagaagata tggattatat tttggaactc gggcgtctct caccaagctc tgtcggttct
      181 gaaccgtgga aatttttagt gctacaaaac ccggaaaccc gtcagaaaat cgccccggtt
      241 tcgtggggaa ttaaacaccc aatggaagaa atgagccatt tagtggtgat tttaaccaaa
      301 aagaatgtcc gttatgattc tgtctatatg cgtgatatgt tagccaaacg aggcttaacc
      361 tatgagcaga ttgaagcctc gctggtacgc tataagtcgt tccaagaaga ggatatgaaa
      421 ttgcttagtg aacgtgaatt attcgattgg agtaccaagc aaacctatat tgcattaggc
      481 aatatgatga ccggtgcggc tctgattggt attgactctt gcccgattga aggttttcat
      541 tacgataccg taaaccaaat tttatcggac gaaggcttat ttgacctaaa cgaatatggt
      601 gtgtcttgta tgataacttt tggctatcgc aacaaagaaa ttaagaaaaa atcccgcaaa
      661 cctaccgaag aagtgattgc gtggattgaa taatcacctt aacttataaa aacgggcgaa
      721 ttaatcgccc atttttatat ctttagtttt gactactcgg ctttcaccca tttattggtt
      781 aatgtgcccg ccaccattgc accattcacg tttaaggcgg tgcgagccat atcaataatc
      841 ggctcaactg aaatgagcaa gcctactaac tcaatcggca gattcaaggt tgatagcacc
      901 acaatcgccg caaaagttgc accaccacct acaccggcaa tgccaaatga ggaaattgcc
      961 accaccaaaa tcaaggttaa aatatagttt aagctaaacg gatccattcc cactgttgga
     1021 gcgaccatca ccgccaacat tgccggataa attcccgcac agccattttg cccgatggtt
     1081 gccccaaagg ttgcagaaaa gttggcaatc acactgttat tacctaattt attggtttgc
     1141 gtctcaatat tcaacggaat ggttgccgca ctggaacgag acgtaaaagc aaaacttaat
     1201 gtcggcaaca cttttttgta gtaatccaac gggttgattt tagccacaaa gagtaaaata
     1261 ccgtgcacaa taaacatcaa accaatcgca atgtatgatg ccacaataaa gctacctaaa
     1321 tttacaatat cgctccattg agaagttgcc gccattttga tcatcaacgc aaatacgcca
     1381 taaggggtta ggcgaatcac aaaacgtact aaacgcataa ccagtttatt taaggtctct
     1441 acaccttgtg cgatacgttc gcccaacgct ttatcttctt tgcctaagct cagtgcagca
     1501 atgcctagca gagccgagaa aataaccgtg ttgatgatcg acgttgggtt tgcaccggtt
     1561 aattctaaaa acggattttt cggaatgaaa gaaagcaaca ttgaaggcac agataagttg
     1621 ctcacctgct ccacacgccc taccactttc tcttgagcgg caagctcacg ggagccggcg
     1681 actaaaccct cggcagataa atcaaacaga tacactaaag caataccaat cgctgcggca
     1741 atcgcggtgg tcactaataa cactgatagc acactaaaac taattttgcc taaagatcct
     1801 gcctctttta agcgggtaat tgccgataaa atagagacaa acaccaatgg cattacgatc
     1861 atttgtagca agcgaacata accgttacca attaagttaa tccaatcaag agttgcatca
     1921 acagtagctt tttcgtaaaa agtttgcaaa aaagcaccgc ttgcaagccc taaaaataac
     1981 ccaataaaca ctgtttgacc gagtttttgc gtagatttat aaattttagc caataaaaat
     2041 aatattgccg caaagacaat taagttgaaa ataagcacag aacctccaaa gtaaaaccgc
     2101 ttattataaa aatgccgcaa gggtattacg cttgcggcat tcattcaaga tgatttatta
     2161 ctttggtata actaaaagtt ataataattc ccagcttaga ttagagatca aacggcaagg
     2221 ctcacatcta aaatggaaaa tatcgtgcaa cggctcagtt aatttccatt ctccgccaca
     2281 acaagggcat ttgcgttgat attcactttc atcacgccct aaacggtaga ggtaataaaa
     2341 ggtgggaatg tgagttttct gctcaatttg ctgacataga tctcgaccga tcattgcaag
     2401 atcgcttgtt tcatcactaa tttgaacaag tgcgtggcac tccagcagat tgccgttcat
     2461 ttgtagcaga tcgcacgctt gccaattttg ctgccattcc aatatcgctt cgctaagaga
     2521 ctgatcctgt aaagaataaa gcggaattgg ggcaaactgc tctccatcat aaagtggcga
     2581 gcaggtatct aaatgggtgg tatataatat ttgaaacgct ggcgagcggt tatgactgct
     2641 ttcttcgccc tgataatctc gcccgacaag ctcaaaactt gcaaaaatta cgccgatttc
     2701 gaccgcttgt tgcaacgctt catttacttc atcactatta tattgtggca aaaggctttc
     2761 ttgtgtaggc acacaaaccc gagcttgaaa ctcgttttga tgaaaagtaa tcggcatttc
     2821 tctgccaatg acttgcccgt tatataacca ttgatttaag atttggctaa ttgccgtcac
     2881 cggatcttgc tcaaaattat gatagctgaa atgcactatc gcctgataca ttaaatattc
     2941 tccgccatat cttgaaattc ttgtttgacc accgtagtcg gctcttgatg aataatcacg
     3001 tgagacatcg gaaatgccgc cagcaatttc tgctcaaggc tatcagttat ttcgtgagct
     3061 actagcagcg ttaagtgatc gtctaattcc aaatgtaatt ggataaaacg cactgcacct
     3121 gcacggcggg ttagaatatc gtgaatccct aaaatgttcg gatgttgcga agcaatctct
     3181 ttaatccgct caatttcttc atcgggcaaa gcttggtcta gcaaggtttg caccgcacac
     3241 caaaacattt ttactgcatt gaaagcaatg tagagggcaa tacctattgc aaaaactgca
     3301 tcggcgtaca caaaaccggc actattaagc aacattgcca ctaaaattgc cgcattcatt
     3361 aataaatcag tttggtaatg taacgaatcc gcttcaatcg ccggactttt cgtaagcgtg
     3421 acaacataac gctgataaaa gactaaaagt gcagtaatca caattgatac aatactgaca
     3481 attaccccaa tactcgattg agcaattaat tccggattgg tgagtttatg gaaaccttgt
     3541 agcagtagga aaaccgccga gccggaaata aacgcacttt gggcaagtgc cgccaaagac
     3601 tccgctttgc cgtgcccaaa cgcgtgattc tcatctgccg gttgtagtgc aaaacgcagc
     3661 actaaaatat tggttagcga agcaaacaga tccactaccg aatccgtcat tgctgccaag
     3721 attgaaatcg aaccggtttt ccaccatgca aaggctttaa tcacaatcag taagatcgca
     3781 acaataatcg ctaaattcgc tgcatttttg actaatttgc tgtatttttc gttcatttac
     3841 tacattattt actgcattgg acttgccaac ccagttggtt taattcaaga gaaagtctag
     3901 caatttcttg gctgtaagcg gtcaaattcg gcgatttttt tgcaactgtc ttgccaactt
     3961 cccgcttatc tgcaaattga atctgcccgt aaccacaagc taaattccgc ccacgggtta
     4021 cttctaacat cacattgtta aaacgttcta taggtggata aatcacactg ctgtgtaatt
     4081 cattttgctg aatttccact ttcgcctcaa gctccggctt tttcgcaaaa cttgcttggc
     4141 ggaatgcagc tcgttgctct aaggtttgat ttttactgtt tttatctaag aaaaataaaa
     4201 tgcctttttg ccaattttcc ggatttttct cactcttttc tggcaaataa agtaaatgtt
     4261 gcatttcatc aatttggctg tgggtgactt ttacaaaagt atcctgccca aagtgaatag
     4321 catcaggcac tgcaaattgt gggctttggc tgacacaacc tgccaatgat aacgcaacca
     4381 aagttattcc ctttttgata gccgacatta tgcctgctcc tcacgtttaa agactagctc
     4441 gctttcacta gaactgtctg catcaaacca atagcccccg tagttaaact gtttgagctg
     4501 ttcgatgcta gtcggttggg tgtctaatat aaagcgaacc atcattccac gggctttttt
     4561 ggcatagaag ctgatcactt tatatttacc gtttttctca tctaaaaaca ccggcttgat
     4621 aatagtcgca tttagatttt gtggcttaac cgcaccataa tattcatcag aggcaagatt
     4681 aactaaaata tcatcgcctt gttcatcaat cgcttgctgc aaattttcag taataatatt
     4741 gccccagaaa gcataaagat ctttgccttt cggattggct aacttagtgc ccatttctaa
     4801 acgataaggc tgcattaaat ctaacggctt taataaacca tacaagcctg aaagcatacg
     4861 caaatgttgt tgagcatatt caacttgtgc tttgcttaac gtttctgcct ctagtccggt
     4921 ataaacatca cctttaaagg caaagagggc ggctttggca ttttgttcgt tatgctcaag
     4981 ctgccactcg gcaaagcgag ccacatttaa agcggctaat ttatcgctaa ttgacatcaa
     5041 actacctaat tcagcaggtg aaagctgttt acaaatatca attaattctt ggctacaagc
     5101 ggtcaaattt ggttgagaaa atgcaaaacc gtcaatattg gttttaaaat caagggtttt
     5161 ggcaggggaa ataatggcta acataaatgc ctctatcaaa tagttaatca gggcggttat
     5221 tctaccacag tcataaagac aaaaaaggca ggaaaccctg ccttcttgaa aacgaaaaga
     5281 aaagtcgggt agcgaaagta cattactgca cctccgcccc ctaagaactg tacaagcaaa
     5341 tttctctgca tacagctcaa gcctttcatc agctatatag ccggcttcac gacctccaag
     5401 ttcaaagtat gaatctttat gtgacaagtc ggatgtacca aacaacgatt gcttaatgca
     5461 tcgcttcctc ctaacatttt gtacacgata tgatgatcgt gccagcctgt ttctttggtt
     5521 attggcaacc cacataatgc acattttcca cgctggtctt tatacagtgc ctgccattgt
     5581 cgtctgtgtg attgttcttc atacagacgt tcttggctca gtttttcgcc gtacaactcc
     5641 cattcaggca aaaacggatt atattcagac ttaattttaa cgtgtcgttt gattttaaca
     5701 tagccacaat agaataagtc tatcggctta gtttctcctt tttcattggt ataaactgtg
     5761 ccaaataccc aatttcgagt cgctgtggta caaaagtatt tctcttttat ccacataata
     5821 cctttattgt gatgacggcg ttcacaccat ttccaaagta ctttccaaac taatgcgtcc
     5881 attcgtccga atgccttact tgcaacttgg cttcgatgat aatttgccca acctcgtaac
     5941 attgggttta gcaccattat aagatcttcc tgtttagcca ttttcattta gaaattacct
     6001 cttttacctt gttgtagaac gctttcacat tcttttgact tggtctgttc agtatttttc
     6061 ctttgaaacg tctcacattc caacctagaa aatcaaaacc ttcttcaata tgtaccactt
     6121 tcgtcttttc tgttgataag gataaacctc gttcattcag aaagttctgg ataagtggga
     6181 taacttgatt ttcaagtaac tctttcgttt tacctgatat gataaagtca tctgcataac
     6241 gtactaagta agttttattt tctcgaattg tccgactgcc ttttcctcca aaatgagttt
     6301 ctaacagttt ttccaatcca tctaatgcca tatttgcaag cgttggcgag ataatcccac
     6361 cctgtggtgt accttcctct gttcgtttca accgtccaaa ttccactaca cctgatttta
     6421 gccatttccg caatatccgt ttattcattg gaatatgctt taatagccag tcgtggctta
     6481 tgtggtcaaa acaacctgca atatccgcat caagtaccca ctcaacaggt tgattagttt
     6541 tcttgccttt atgcgaataa atttagtgta tatgagtaat agcatctacc gttgaacggt
     6601 ttaatctaaa tccataagaa cctttatccg cagttgtttc tgccacaggt tgtattgcca
     6661 ataaataaag ggcttgcatt gctctatctc gcattgttgg aatacccaac ggacgtttct
     6721 ttccattcgg tttggggata tacactcgtc ttaatggact aggtttatac cctttttccg
     6781 tcaatgatag tgcggcttgc cattttgtct ctggggtctc ccaaagctct ttatcgacac
     6841 cacttgttcg cttaccttga ttttcagtca ctcttcttac tgcaactaat ttagcataat
     6901 gtgagtgggt tagcattcgt tgtaagtttt taaccttacg ccaattgcct tccagtgttg
     6961 ccttcgcaat ccgaatttgc attcctttca catacgtttc agctttatgc caatcgacat
     7021 aatgccattt ggagatgtgc gaggtcgctg gattgcaagc taacttttca gtataatgct
     7081 tacgctcttt catacctacc tctgatatta caagataagt cgaaagtctc agcaagtggg
     7141 tagatccttc aagtaaacaa atccctattg agataaacta tccttagttt attccctttg
     7201 gttacaagag gatcttaccg ttttaccgat aatttcaacc gctcttgtaa ccgattgggt
     7261 taaagagtcg agcgctaggt tatgatgagc ctaacgctca tttctttaat ttgtttttgt
     7321 acctattcaa cattgtattc cttgataaca ttcaatttct cttctgttat ctacaatctc
     7381 tttcggtgtt acccttaaag gaaacgctat tcatcttacg atgaaagacc agttagaagt
     7441 ctgcaccctt tcgggttagg ctatgtttca caccctatct atgccattac agcatagcat
     7501 tcgctttttc taacctccct tacctgcatt cccaacagta tcgcttacgc tttacttgcc
     7561 ttttcctctt ttaccttcgc catctatttc aaacggtttg agtattgtgg gcaaggcaga
     7621 aatacaggct taccaagttc cattactatc acacgagtag tgaagataca gtctctcccc
     7681 cgacagtgct ttgatggcgt atcctcacgt taaagaagaa taaccgactg cctacctttt
     7741 ggtcagagcc tatcaatagc tttggctctc tctcgataac gaggtttatc aactgttcac
     7801 ttacgtttat ctttctaccc agtctagccc cttgaaggtg tgctattcac tttctcccaa
     7861 tgtcctctca cgatttcatt gactgatttt catcaggggt tcattgtcag gaagctccgc
     7921 accacaccgt taccggtgtc gcactatccc tagactactg ttagccatat aacaggttac
     7981 cttatcattc cctccgtact actcatatct tcggattgtt tgaccgtaac aatgacagta
     8041 atagcttacg catacttact aagtcagtat cctatactgc ataaataagt ccgaaccttt
     8101 acaaaataaa ttcacttatg cgactaaagt cgctttgtct atatccaaat tttcccgcaa
     8161 atagcctata acctgtccaa taggcgaagg tagctaaccc tagctaatgt gagtattaga
     8221 ggtgatttta acgagaattt aaggtataat tatccctagc tcataaaaac gaataaacaa
     8281 gagtttatcc ttagtgtttt tattcactat tgaggagcat tctacgatta ccgtagattg
     8341 cgagtgtgaa aagtataagt aaagatggca ctccttccct aagatttacc aagtcttagt
     8401 tctgatgtct tttctgagtg ctgaggcgat ctattggact ttatttatag taggtgttgt
     8461 aaattttctc tgctctgggg tatgggtaac tccggtctgc ggatcttgct ttcttactct
     8521 atcttggtat taagaaaaaa accaagtaca tcaaagtgaa gatcatacct tacagccttt
     8581 cgagttggtt catttacttt cccaacttag aacaagagtg cttgggcaaa gccccttttc
     8641 tcttgcctgt ctttccaagc actccgaaga gtgcttggaa agtcggaagc aggtgataag
     8701 aataccaccc acttccagca ataactcctt gatatataag gagttattgc cataagtacg
     8761 acaacttgtc gcacttaacg ttttgagtat tgtggacgac gacgtgcttt gtgtaaaccc
     8821 acttttttac gttcaacgcg acgtgcgtca cgagtaacga agcctgctgc acgaagtgct
     8881 gggcgtagcg tttcatcgta ttccattaat gcacgagtga taccgtgacg gattgcaccc
     8941 gcttgaccag aaataccacc acctttaaca gtgatgtaaa ggtctaattt atcagttaat
     9001 tcaaccaatt ctaacggctg acgtacgatc atacgagctg tttcacgacc gaagtaaact
     9061 tcaagtgaac gttggttgat agtaatgtta cctgaacccg gtttgataaa tacacgggct
     9121 gaagagcttt tgcggcgacc tgtgccgtag ttttgatttg ctgctgtcat ttttatgctc
     9181 cgtgaattag atatctaaaa cttgtggttg ttgtgcttgg tgattgtgtt ctgaacctgc
     9241 gtaaactttt aatttacgga acatttcacg accaagtgga ccttttggta acataccttt
     9301 cactgcgatt tcaatcacag cttcaggacg acgagcaatc atttctttga aggttgcatc
     9361 tttgatgcca cctacatagc cagtgtgcca gtagtagatt ttgtcagttt gttttttacc
     9421 tgttactgct actttgtctg cattgatcac gatgatgtaa tcaccggtat caacgtgtgg
     9481 agtgtactca ggtttatgtt taccacgaag acggcgtgcc aattcagtcg ctaaacgacc
     9541 taatgtttta cccgtcgcat ctactacata ccagtcacgt tgtactgttt ctggttttgc
     9601 tacaaaagtt ttcattaatt aaataccaaa tttaaagttt aaaacccaat acctcattga
     9661 ggtatttcct attattttac ggtgaacccg accccttcga gtctgattca gaccaaactt
     9721 gtgtggcggg gaaaaccaca caaaaacagg gtggcgaaat tatacagaga tttcactcaa
     9781 aaatctaaca ttttttgctg ataatctttc aaaattgaac ctatatcaca ttttaatcat
     9841 ttggagcttt tcaaataaaa actgaaatgt taaaaatagc agtaatcaat cactaaaaca
     9901 taaggagttt atgatggaac aatggacaac agatgtttgg gcagccatta tggcagcatt
     9961 tattgtaggt gctgtaatag gttgcactct agttagagta tttaacagta gtgtcagaaa
    10021 acagcatgaa ctggaaaaag agttgaagca aaccaaaact caagtggatg agcaaaaaca
    10081 aaaacttgaa caacactttg agcaatcagc ccaattactt tcaagtctag cttctgatta
    10141 taaaaagctt tatacccatt tagcacaaag ctctgaaaag ctattaccag aagcatcaaa
    10201 aaatattacc tttttccaac aaaccaaatt acctattccg gcagaaaaaa cggaagaaga
    10261 tcaacctaga gattattccg aaggttcatc tggcttactg aaatcataaa actaaaaatt
    10321 gaatatttag ttttactaaa aggcacttag tttaaggatt gattccgttg gaatcaatcc
    10381 tttattctaa accgctacat taagcagtta acaacatttt tcagctttag atactgaact
    10441 taggttataa gaagcctatt ttgtaagagt tattcccact caatggttgc cggtggtttt
    10501 ccgctcacgt catacaccac acgggaaata ccgttcactt cgttaataat gcggttggag
    10561 attttgccta atagatcata cggcaagtgc gcccagtgag cggtcataaa gtcgatggtt
    10621 tccaccgcac gcaggctgac gacccagtca tatttgcgtc catcgcccat tacgcccacc
    10681 gatttcaccg gcaagaatac cgtgaaagct tggctgactt tgtagtacca gccggctttg
    10741 tgcagttctt cgatgaagat cgcatcggct ttacgcagta aatcgcagta ctcttttttg
    10801 atttcgccca gcacacgcac gcctaagccc gggccgggga acgggtggcg gttgagcatt
    10861 tcagccggta agccgagtgc caagccgatt ttgcgcactt cgtctttaaa caattcacgc
    10921 aacggttcga ctaagccgag cttcatataa tccggcaagc cgccaacgtt atggtgtgat
    10981 ttaatcacgt gggctttgcc ggttttgctg gcggcggatt caatcacatc agggtagatc
    11041 gtgccttgtg caagccattt gaccgagcta agttttttgc tttcgtcatc aaacacgtcc
    11101 acaaacactt taccaatggt tttgcgttta gcttccggtt cgtctaagcc ttttaacgct
    11161 tctaagaaac ggtcttcggc attcactcgc acaatgttca aaccgaattt gttgccgaac
    11221 atttccatga cttgatcgcc ttcgttcaag cggagcaagc cgttatccac aaatacgcag
    11281 tgcaggtttt taccgatagc acgatgtaat agcaaggcgg tgacggagga atccaccccg
    11341 cccgacaaac ctaaaatcac ctcgtcatcg cccacttgct cacgaatgcg tttcaccgca
    11401 tcttcaatga tgttttctgc cgtccagttc gtttcacagc cgcaaatgcc gaccacgaag
    11461 tttttcagca acgccaagcc ttgtttggtg tgggtcactt cggggtggaa ctgcacgcca
    11521 tagaaacggc ggctttcgtc cgacatcgcg gcaatcgggc aggttggggt aacgcctgtg
    11581 atggtaaagt gcggtggtaa tttggtgact ttgtcgccgt gactcatcca cacgtccaat
    11641 tttggctcgc aagcggtcaa atcatcgttt aaatttgcaa aaagttgatc ggcagatttg
    11701 agagccaccg aggcatagcc aaattcacgg tggtcggacg tttcggttaa accgcccaac
    11761 tgcatcgcca ttgtctgcat tccgtagcaa atgcccagca ctggcagacc agcgttaaac
    11821 acatactccg gcgctcgtgg gctgttttcc tcagtggtgc tttcaggacc tcctgaaaga
    11881 ataatccccg ttgggttaaa ttcacggatc tgctcttccg ttacgtccca agcccaaagc
    11941 tcgcagtaaa ccccaatctc ccgcacacgg cgtgcgataa gctgagtata ttgtgaaccg
    12001 aagtctaaaa ttaaaatttt gtggtggtgg atgtttgtca ttgtaatttc ctttaaatta
    12061 tggcgttgtg gtcatgttaa taaaagtgcc gcctacccca tcgtttctat atgtaataat
    12121 aaaagattgc gttttattac aacctgttgc agtccaaagt tcttttgcat taccttcact
    12181 tggaatattg atgatttttg tatgaatttt atcaggagta catttatgta ttgcttgaaa
    12241 atacagattt atatttcttt ctgtgtccaa ctttaataaa ggactggccg tt
//
LOCUS       DANLXS010000066        12043 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.066, whole genome shotgun sequence.
ACCESSION   DANLXS010000066 DANLXS010000000
VERSION     DANLXS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12043
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.066"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            44..1042
                     /gene="yiaK"
                     /locus_tag="PXD71_001322"
     CDS             44..1042
                     /gene="yiaK"
                     /locus_tag="PXD71_001322"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDL6291445.1"
                     /translation="MRVSYEELKAQFKRVLLARNVREEIAEECATVFADTTQAGAYSH
                     GVNRFPRFIQQLENGDIVPEAVPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMELAS
                     ENGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
                     IAVPTNPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNLRL
                     MPMGFWKGSGLSIVLDMIATLLSNGESTAAVTEDKDDEYCVSQVYIAIEVDRLIDGKT
                     KDEKLNRIMEYVKTAERADPDVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQAL"
     gene            1167..1532
                     /locus_tag="PXD71_001323"
     CDS             1167..1532
                     /locus_tag="PXD71_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL6291446.1"
                     /translation="MFFGHISQVKANRYPPAIQIALNYLKNTDFDAMEAGVYELKGRQ
                     IYVQVLDLNTKSKHEFQPEVHRNYLDVQYLHRGKEIMAAAVDTGTNPIAMEYNPERDI
                     QYYQSVANENEFRCVEGNF"
     gene            1596..2075
                     /locus_tag="PXD71_001324"
     CDS             1596..2075
                     /locus_tag="PXD71_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL6291447.1"
                     /translation="MKALAQLVGKLLEILVVVILSAMSILVFVNVVLRYGFNSSISVT
                     EEVSRYMFVWLAFLGAVLAFSENQHVKVTMLTDKLSPTARNILSVVTDLAMLFCCYLI
                     VDGSWIQFNLNLNNFAPISGLPQGITFLASTVAGVLIGLLIIARIVATVGVIAKGEK"
     gene            2076..3353
                     /locus_tag="PXD71_001325"
     CDS             2076..3353
                     /locus_tag="PXD71_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL6291448.1"
                     /translation="MTVVIFLSVLLGSILLGIPVAFALLVCGVALMLHLDLFDAQILA
                     QQIVSGADSFSLMAIPFFILAGEIMNEGGLSKRIIDLPMKLVGHKRGGLGFVAILAAM
                     IMASLSGSAVADTAAVVAMLLPMMKTTGYPIDRSAGLIGTAGIIAPIIPPSIPFIVFG
                     VASGVSITKLFLAGIFPGIMMGICLAALWWWQAKRLDLMTFSKATKEELCISFKNSIW
                     ALLLPVIIIGGFRSGMFTPTEAGAVATFYALIVSLFVYREMKFKDLYKVVLAAAKTTA
                     VVMFLVAAANVTGWLITVAELPMMLTELLEPLIESPTTLLLVIMLAVFIIGMVMDLTP
                     TVLILTPVLMPLVEEAGIDPVYFGVLFILNTSIGLITPPVGNVLNVITGVSKLPFDQA
                     AKGVMPYLVMMICLLLLFVFFPSLVLVPLSWMQ"
     gene            3456..4442
                     /locus_tag="PXD71_001326"
     CDS             3456..4442
                     /locus_tag="PXD71_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL6291449.1"
                     /translation="MKLFNLKTLFALVAGVAVMSGSAMAETSLRFGYEAPRSDTQHIA
                     AKKFDELLAAKTKGEIKLKLFPDSTLGNAQTMISGVRGGTIDIEMSGSPNFTGLEPKL
                     NVIDIPFIFKDREHVYKVLDGEVGQGLLKDLEAQGLKGLAFWDVGFRAFSNSKHPVTK
                     PEDIKGLKVRTNQNPMYIQAFTLLGGNPVPMPLSELYTALETRAVDAQEHPIGIFWSA
                     KLFEVQKHLSLTNHGYTPLIVVMNKAKFDGLAPELQTALVEAAKEAGQFQRDLNVKNE
                     KEIIEKLRKQGIEVIEQVDNAPFKAAIEAEVRKAFVEKHGDELLKKVDALAQ"
     gene            4516..5499
                     /locus_tag="PXD71_001327"
     CDS             4516..5499
                     /locus_tag="PXD71_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL6291450.1"
                     /translation="MKLFNLKTLAAFVAGFALFSTAQAETSFRFAYEAPRSDTQHIAA
                     KKFNDLLQEKSQKGLKLSLFPDSTLGNAQTAISGVRGGTIDIAMSSSSNFTGLEPKLN
                     VIDIPFIFKDRDHAYKALDGEIGQGLLTALEAHGLKGLAFWEVGFRAFSNSKHPITKP
                     EDIKGLKIRTNQNPMYIQAFSILGANPVPMPLSKLYTALETKAVDAQEHPIGIFWSAK
                     LYEVQKYFSFTNHGYTPLIVAINKAKFDALPAEQQTALVEAAKEAGKYQRQLNLENEQ
                     KIIDSLKKAGIEFVDNLDTTPFKDAVSAETRKAFIEKNGDGLVKAIDALAK"
     gene            5630..7114
                     /locus_tag="PXD71_001328"
     CDS             5630..7114
                     /locus_tag="PXD71_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6291451.1"
                     /translation="MQHIIKRFGSHTAVNDVSLDIHSGEIVALLGENGAGKSTLIKIL
                     AGIYDRDEGDILFHNQKIQSAHAIEKGNKKPIAFIHQDLGLIEWMTIAENMAFVMGFP
                     RKFGLIDWAKVREQSQKALDFVGIKLSADTRVFDLSRTEKALLAIARAIAVNAEILVL
                     DEPTASLPASDVEHLFTVLNRLRSQGVGMIYVTHRLDEVIAISDRILVMRDGFPVAEG
                     QTKLYDVKGLVKAIVGEESRGRQRVDLPTDTPEILSLQNVVVGDTGPVNFRLHKGEMI
                     ALAGLRGAGQEEIGRLLFGERTLDKGEIQLDKRSYSAKDPQDAIAKGVALVAGDRTRE
                     SVFMSMTTSENLFINPVLSGHSPLKRYSRREEWGESWYKFQLFDIRPKNLFIDASALS
                     GGNQQKIVLARWMHLNTPVLILEDPTAGVDVGARAEIYDLLNKALAEGVAIIVISTDF
                     EEIAHLCNRALVFNRGEIAGELLNEEVTFANLLALASDSQSKKQ"
     gene            7309..8355
                     /locus_tag="PXD71_001329"
     CDS             7309..8355
                     /locus_tag="PXD71_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6291452.1"
                     /translation="MAQTNIKSTALEQPVSIARDGFMAWFSQMSTRYGLLWLCVLLVI
                     IFSLTTESFASMLTLNAILESKSKIALLALAATTTMIVGKIDLNVGFGIVLWHILVIT
                     LQVQYGFSWYMAILIVLVIAALYGLLNGILVALADIDSFVATLGSGTVLYAVALWHSG
                     GRQIVGDLPDEFIALNSTEIFGIPISAFYVLAVAIVMWLVTEHTPTGRCMYAVGGNPT
                     AARLNGINIKKYTIVPFIVSSVITAFTGVLISAQQGVGQASVGMDYLLPALVGAFLGS
                     TTIRPGRINVWGTVVGIAILAIGISGIQQFGGAFWVEPLFNGATLLLSITIAGYAQRK
                     RLLNQKAVQKKSTN"
     gene            8368..9477
                     /locus_tag="PXD71_001330"
     CDS             8368..9477
                     /locus_tag="PXD71_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL6291453.1"
                     /translation="MKKAFRTTLTAIATLTAIAFGNNAFANDDPFVQEAKQLVAAATA
                     KQEKWDGPTTGPQLQKEKTVIFIASDMKNGGVLGVIDGMKEAVGVTGWKLDVLDGAGT
                     VNNQLAALNQAIARKPDAIVIGGWNPNVAKIPLQKATKNGIKLAAWHATPEPGAIDKY
                     GIFYNVTSDSDEIARLSAMLAVAESNGKAKAIILTDSLYEIALRKANVMKEVIEKCKE
                     CKVLEFIDTPLADTSSRMPNLTFSLLQKYGDDLEYALAINDLYFDFMAPSLRTAGKDK
                     PFSISAGDGSVTAYQRIRNGDKQFATVPEPLNLHGWQLVDELNRAFANEKPSGYITPA
                     HLVTKENVAFDGGENNLYAPQNGYKGAYKAIWGVK"
     gene            9582..10472
                     /locus_tag="PXD71_001331"
     CDS             9582..10472
                     /locus_tag="PXD71_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDL6291454.1"
                     /translation="MSNKFIPKISNPRFNELVGNTFELEELFDKAIWAEGPAWSKPQQ
                     ALYFSDVKGCTMYRWTEQDGTQVFRQPSYFSNGNAFDANDNLITCEHGNRGISLTTPS
                     GEYRFLVGQLDGKRLNSPNDVVVKSDGIIWFTDPPYGILSDNEGKQSPSEIIGCYIYC
                     YDPKTDKLNIATFNTMRPNGLAFSSDEKQLFVADMSSVEFDEDGLHHLVVFDVDGNKL
                     KNRRDVAEISPGIPDGFRIKQNGVIFCSCEEGIIAILPDGTELGRFVLGKLTSNCSFG
                     DDEQTLFITCSNSVYRLTIK"
     gene            10544..12013
                     /locus_tag="PXD71_001332"
     CDS             10544..12013
                     /locus_tag="PXD71_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDL6291455.1"
                     /translation="MDNKMNYTLGIDCGGTFIKAALFDTQGNIQALHRENVSVISEQA
                     GYAERDMSQLWAICAEVVRSTIEKSGVNPADIKAVGISAQGKGAFLLDKQNRPLGRAI
                     LSSDQRSLEIVKQWQAEGIPQQLYPITRQTLWTGHPVSILKWVQQNEPERYAQIGAVL
                     MSHDYLRFCLTGELHCEETNISESNLYNMEKGEYDQQLADLLGMPDVLEKLPPVIEPN
                     KISGYVTENAAKLTGLAVGTPIVGGLFDVVSTARCANLDDETKLNAVLGTWSVVSGIT
                     DHIDASQSLPFVYGRYAEAGKFIVHEASPTSAGNLEWFVNQWKLDYVKINQAVAQLPP
                     AASSVLFVPFLYGSNAGLGMQAGFYGMQAHHTQAHLLQAIYEGVLFSLMSHLNRMFIR
                     FPKADVLRVTGGPVKSKVWMQMLADLTGMRLEIPQMEETGCLGAALMAMQGVGLDISQ
                     VQALNNEMQVFEPNPENYAAYQTKYQRYQKLTEALNTMI"
BASE COUNT         3341 a         2597 c         2910 g         3195 t
ORIGIN      
        1 acgtgatttt actatttgaa atattgaggt attcaagatg actattcgag tttcctatga
       61 agaattaaaa gctcaattta aacgtgttct gttggctcgc aatgtgcgtg aagagattgc
      121 cgaagagtgt gccactgtgt ttgccgacac cactcaagcg ggtgcttatt cacacggggt
      181 aaaccgtttc ccacgcttta ttcagcagtt agagaacggt gatattgtgc cggaagccgt
      241 gccgactaaa gtgctttcac tgggggcgat tgagcagtgg gacgctcacc aagccatcgg
      301 taacttaacc gcaaaaaaaa tgatggacag agcaatggag cttgcatctg aaaacggcat
      361 cggcgtagtg gcgttacgta atgcaaacca ctggatgcgt ggcggttctt acggatggca
      421 ggcggcagag aaaggctata tcgggatctg ctggacgaac gcccttgcgg taatgccgcc
      481 ttggggggcg aaagagtgcc gtatcgggac taacccgtta atcattgcgg tgccgactaa
      541 cccaatcacg atggtggata tgtcttgctc aatgtactca tacggaatgt tggaagtgca
      601 tcgccttgct ggtcgtcaaa cctttgtcga tgctggtttt gatgatgacg gcaacccaac
      661 ccgtgatccg gcaacggtcg agaaaaacct ccgtctaatg ccgatgggct tctggaaagg
      721 ttcgggctta tcgattgtgt tagatatgat cgccaccttg ctctctaacg gtgaatccac
      781 tgcggcagtc accgaagata aagacgatga atattgcgtg tcgcaagttt atatcgctat
      841 tgaagtggat cgcttaattg acggcaaaac caaagacgaa aaactcaacc gtattatgga
      901 atatgtgaaa accgcagaac gtgccgaccc tgatgtggca gtacgtttac cgggccacga
      961 gttcacccaa attctagccg ataacaaagc caacggcatt ccggtggatg acaccgtttg
     1021 ggcgaaatta caggcgttgt aattgcaaat tgaccggtag ggtgggcgta agcccacgca
     1081 ttagacacaa tcataagatg acgtgggctt acgcccaccc tacaagcggt catttttccg
     1141 cagaatttta caaaacggag gtatgaatgt tttttggtca tatttcacaa gttaaagcga
     1201 atcgataccc gccggcgatc caaattgcgt tgaactatct gaaaaatacc gattttgatg
     1261 cgatggaagc aggggtttac gaactcaaag gcagacaaat ttatgtgcag gtgttggatt
     1321 tgaacaccaa atcgaaacac gaatttcagc cggaagtgca tcgcaattac ctcgatgtgc
     1381 agtatctaca ccgaggcaaa gagataatgg ctgcggcggt ggataccggc accaatccga
     1441 ttgcgatgga atacaaccct gagcgggata ttcaatatta tcaatccgtt gccaacgaaa
     1501 atgaatttcg ctgcgttgaa ggcaattttt gacggcacgg aaaccattcg caaagtggtg
     1561 gtaaaaattg cgatgagcga aattgaggag caacgatgaa agccttagct caattagtcg
     1621 gaaaactgtt agaaatcttg gtggtggtga ttttatccgc aatgtcgatt ttggttttcg
     1681 tcaatgtggt actacgttac ggttttaaca gcagcattag cgtgaccgaa gaggtgtctc
     1741 gctatatgtt tgtttggttg gcgtttttag gggcggtgtt ggcatttagc gaaaatcaac
     1801 acgttaaagt cacaatgctg accgataaat tatcgccaac ggctcgtaat atcttaagcg
     1861 tagtcacaga tttagcgatg ttgttctgtt gctacttaat tgtggacggt agctggattc
     1921 aatttaacct caacctgaat aactttgccc cgatttcagg cttaccgcaa ggcatcactt
     1981 tccttgccag cacagtggca ggggtattaa tcggtttatt aattattgcc cgcattgtgg
     2041 caacggttgg cgtgattgca aaaggagaaa aatagatgac tgtggtaatt tttctttccg
     2101 tcttgcttgg ctcgatttta ttgggcattc cggtagcttt cgccctgttg gtctgcggtg
     2161 tggcgttaat gttgcattta gatctgttcg atgcccaaat tttggctcaa caaattgtga
     2221 gcggtgcaga cagtttctcg ctaatggcaa ttccgttctt cattcttgcc ggtgaaatta
     2281 tgaacgaggg cggtctctcc aaacggatta tcgacctgcc gatgaaatta gtcggtcata
     2341 aacggggcgg tttaggtttt gtagcaattt tagcggcgat gattatggca agcctttccg
     2401 gttcggcggt ggcggatacc gcagcggtag tcgcaatgct actgccgatg atgaaaacca
     2461 ccggttatcc gattgaccgc tctgccggtt taatcggtac ggcagggatt atcgccccaa
     2521 tcattccgcc aagcattccg tttatcgtat ttggtgtggc tagtggcgtg tccattacca
     2581 aactgttctt agccggtatt ttcccgggca ttatgatggg gatctgcttg gctgcccttt
     2641 ggtggtggca ggcaaaacgt ttggatttaa tgaccttctc caaagcgacc aaagaagaac
     2701 tttgcatttc atttaaaaac agtatttggg cgttattgtt gcctgtcatt attatcggtg
     2761 gtttccgctc gggaatgttc accccaaccg aagcgggtgc ggtcgcaact ttctatgcct
     2821 tgattgtttc actgtttgtt taccgtgaaa tgaagttcaa agatttatac aaggtagtgt
     2881 tggcggcagc gaaaaccacg gcagtagtga tgttcttagt ggcagcggca aatgtgaccg
     2941 gctggctgat taccgtagcg gaattaccga tgatgctcac cgagctgtta gagccgttaa
     3001 ttgaaagccc gaccacactc ttattggtga ttatgttagc ggtgtttatt atcggaatgg
     3061 tgatggactt aaccccaacc gtgctgattt taacgccggt attaatgccg ttggtggaag
     3121 aggcaggcat tgacccggtt tacttcggtg tgctgtttat tcttaacacc tccatcggct
     3181 tgattacacc accggtcggc aacgtgctaa acgtgattac cggcgtgtcg aaactgccgt
     3241 tcgaccaagc ggctaagggc gtaatgcctt acctcgtaat gatgatttgt ttactactgt
     3301 tgttcgtgtt cttcccgtca ctggtgttag tgccgttaag ctggatgcag taatggtttg
     3361 aaccgaaaca agcggtcgaa ttgacgcaaa aatttgcaaa tttttttggc aaaacagacc
     3421 gcttgcaact caatttttaa tcacaggaga accctatgaa attatttaat ttaaaaacct
     3481 tatttgcctt agtggcaggc gttgcggtaa tgagcggctc tgcaatggca gaaacctcac
     3541 tgcgttttgg ttatgaagcc ccacgttccg atacccaaca catcgccgcc aaaaaattcg
     3601 atgaattatt ggcagcaaaa accaaaggtg aaattaaatt aaaactgttt ccggacagca
     3661 ccttaggtaa tgctcaaacg atgatcagcg gcgtgcgtgg cggtactatc gacattgaaa
     3721 tgtccggctc gccaaacttt accggcttag agccaaaact aaatgtgatt gatattccgt
     3781 ttattttcaa agatcgtgaa cacgtttaca aagtattaga cggcgaagtt ggtcaaggct
     3841 tattaaaaga tttagaagcc caaggcttaa aaggcttagc cttctgggac gttggcttcc
     3901 gtgctttctc caactcaaaa caccctgtga ccaagccgga agacatcaaa ggcttaaaag
     3961 tccgcaccaa ccaaaacccg atgtacattc aagccttcac cttactaggc ggcaacccgg
     4021 tgccaatgcc actttctgag ctttacaccg cactcgaaac ccgtgccgtt gatgcccaag
     4081 aacacccaat cggcattttc tggtcggcaa aactgtttga agtacaaaaa catttaagtt
     4141 taaccaacca cggctacacc ccgttaatcg tggtgatgaa caaagcgaag tttgacggct
     4201 tagctcctga attacaaacc gcattagttg aagccgcaaa agaagccggt caattccaac
     4261 gtgatctcaa cgtgaaaaac gaaaaagaga tcatcgaaaa attacgcaaa caaggcatcg
     4321 aagtgatcga acaagtggat aacgccccgt tcaaagcagc gattgaagcg gaagtccgca
     4381 aagctttcgt ggaaaaacac ggcgatgaat tattgaaaaa agtggatgcg ttagcacagt
     4441 aattgacttt attttagtct aaagctccct ccgtttgggg ggagttgttg tatagactct
     4501 actataggag agactatgaa actgtttaat ttaaaaactt tagccgcttt cgttgccggt
     4561 tttgcactgt tttcaaccgc acaagcagaa acctcattcc gctttgcgta tgaagctcct
     4621 cgttctgata ctcaacatat tgcagcgaaa aaatttaatg atttgctgca agaaaaatcc
     4681 caaaaagggc tgaaattaag cctcttccct gatagcacgc tcggcaacgc acaaacggcg
     4741 attagcggtg tgcgtggcgg tactattgat attgcaatgt ccagctcctc taactttacc
     4801 gggttagaac caaaactgaa tgtgattgat attccgttta ttttcaaaga cagagaccac
     4861 gcctataaag ccttagatgg agagattggt caaggtttat taaccgcatt agaggctcac
     4921 ggtttgaaag ggttggcgtt ttgggaagtt ggtttccgag cgttttccaa ctccaagcac
     4981 ccgatcacca aaccggagga tattaaaggc ttaaaaatcc gtactaacca aaacccgatg
     5041 tatattcaag ccttctctat tttaggtgca aatccggtgc caatgccgct ttccaagctt
     5101 tacactgcac tggaaaccaa agcggttgat gcccaagaac acccaatcgg cattttctgg
     5161 tcggcaaaac tctatgaagt acaaaaatat ttcagcttta ctaaccacgg ttacaccccg
     5221 ttaattgtgg cgattaacaa agcgaaattt gatgcactgc cagccgagca acaaaccgca
     5281 ttagttgaag cggcaaaaga agccggaaaa taccaacgcc aacttaacct agaaaatgag
     5341 cagaaaatta tcgactcgct caaaaaagca ggtattgaat ttgtggataa cttagatacc
     5401 acaccgttca aagatgccgt atcggctgaa acccgcaagg catttatcga gaaaaacggt
     5461 gatggcttag ttaaagccat tgatgcattg gcgaagtaaa aatagagcta caaagtgctc
     5521 ggttaaacta acctcacacg aatcgtgtga ggtacaagcg gtcaaaatcc gcaaaatttt
     5581 tacacatctt tcctcaaagg aggaacaaat ggcacagccc attttaagta tgcagcacat
     5641 catcaaacgc tttggtagcc atacggcggt gaatgatgtc agccttgata tccactcagg
     5701 cgagatcgtt gccttactcg gtgaaaacgg tgcaggtaaa tcgaccctaa tcaaaatcct
     5761 tgccggtatt tatgaccgtg acgagggtga tattcttttc cataaccaaa aaatccaatc
     5821 tgcccacgct attgagaaag gcaacaaaaa gcccatcgct tttatccacc aagatttagg
     5881 cctaatagaa tggatgacga ttgccgaaaa tatggcattt gtaatggggt tccctcgcaa
     5941 atttggcttg attgactggg cgaaagtgcg agagcagtcg caaaaagccc ttgattttgt
     6001 aggcattaaa ctctctgccg acactcgagt gtttgattta agccgtaccg aaaaagcctt
     6061 actcgccatt gcccgtgcga ttgccgtcaa tgcagaaatc ttggttttag acgaacctac
     6121 tgcttcccta cctgcctctg atgtagagca tcttttcact gtgttaaacc gcttacgttc
     6181 ccaaggtgtg ggaatgattt atgtgaccca ccgtttagat gaagtgatcg ccatttccga
     6241 ccgcattttg gtgatgcggg acggtttccc ggttgcagaa gggcaaacca agctctatga
     6301 cgtcaaaggc ttggtcaaag cgattgtagg cgaagagagc cgtggcagac agcgggtgga
     6361 tttaccaacc gatactcctg aaattttaag cctgcaaaat gtcgtggttg gcgacaccgg
     6421 cccggtgaat ttccgtctgc acaaaggtga gatgattgcc cttgccggct tgcgtggagc
     6481 agggcaggaa gaaatcggcc gcttattatt tggggagcga accttagaca aaggcgaaat
     6541 tcagttagac aagcggtcat attctgctaa agatccgcaa gatgcgatag ccaaaggtgt
     6601 ggcattggtt gcgggagacc gcacccgtga aagcgtgttt atgtcaatga ctaccagtga
     6661 gaacctgttt atcaacccgg tactaagcgg tcattcacca ttaaaacgct actcccgccg
     6721 tgaagagtgg ggtgagagct ggtacaaatt ccaactattc gatattcgtc cgaaaaattt
     6781 gtttattgat gccagtgcgt tatccggtgg taatcagcag aaaatcgtgt tggcacgttg
     6841 gatgcacctc aatacgcctg ttctgatttt agaagacccg actgccggcg tggatgtagg
     6901 agcaagggca gagatttacg acttgctcaa caaagccctt gcagaagggg tcgcaattat
     6961 tgtgatttcc accgactttg aagagatcgc tcatttgtgt aaccgagcat tagtcttcaa
     7021 ccgaggggaa attgccggtg aactactgaa cgaagaggtg actttcgcca atttgctggc
     7081 gttggcatcg gatagtcagt cgaaaaaaca ataacttact ctcgcccgct tgcgggagag
     7141 agacagctaa aaatagcgtc aggctatttt tagcagagag agggtaaaca agcggtcatt
     7201 tttcttaaaa aatattgcga accctctccc tccgaaagcc taacggcttt ctcctccctc
     7261 tcccgcaagc gggcgagggg aaaatgcaga attttaacgg agtaatttat ggctcaaaca
     7321 aacatcaaat caaccgcact ggaacaaccg gtatctatcg cccgtgacgg ttttatggca
     7381 tggttttcac aaatgtccac tcgttatggc ttactgtggt tatgtgtgtt attggtgatt
     7441 attttctcac ttaccaccga atctttcgcc tcaatgctga cattaaatgc gattttagaa
     7501 agtaaatcga aaattgccct acttgctttg gcagccacaa ctacaatgat tgtcggcaaa
     7561 atcgacctga acgtgggttt cggtattgtg ttgtggcaca ttcttgtgat tactctgcaa
     7621 gtccaatacg gcttctcgtg gtatatggcg atcttaatcg tgttagtgat tgccgctctt
     7681 tacggcttgc tgaatgggat tttagttgct cttgctgata tcgatagctt tgtggcaaca
     7741 cttggctcag gcacagtgct ttatgccgta gcactctggc actcgggcgg tcgccaaatt
     7801 gtcggggatt tacccgatga atttatcgca ctcaacagca cagaaatttt cggtatcccg
     7861 atttctgctt tctatgtgct ggcagtggct atcgtaatgt ggttggtgac agaacatacc
     7921 ccgaccggtc gttgtatgta tgcggttggt ggcaacccaa cggcagctcg cctaaatggt
     7981 atcaatatta aaaaatacac cattgtgccg tttattgtct ctagtgtaat cactgctttc
     8041 acaggcgtat tgatctctgc tcaacaaggc gttggtcagg caagtgtcgg tatggactac
     8101 ttactgcccg cattggttgg ggcattctta gggagtacca ctattcgccc agggcgtatt
     8161 aacgtatggg gaacagtggt ggggattgcc attcttgcca tcggtatttc aggtattcaa
     8221 caattcggtg gagcgttctg ggttgagcca ctctttaacg gtgcaacact gttactttcc
     8281 atcactattg caggctatgc ccaacgcaaa cgcttgttaa atcaaaaagc tgtacagaaa
     8341 aaatcgacca actaagagga tttgattatg aaaaaagcat ttcgtacaac cttaactgca
     8401 atcgcaacct taactgctat tgctttcgga aataatgcgt ttgccaatga cgacccgttc
     8461 gtacaagaag caaaacagct tgtcgcagca gctacggcta agcaagaaaa atgggacgga
     8521 ccgacaaccg gccctcaatt acaaaaagag aaaactgtga tttttattgc ctctgatatg
     8581 aaaaacggtg gtgtacttgg tgtgattgac ggaatgaaag aagctgttgg cgtaacgggc
     8641 tggaagttag atgtattaga cggtgcaggt acggtaaaca accaactcgc cgcacttaac
     8701 caagcgattg cccgcaaacc ggatgcgatt gtgattggtg gctggaaccc gaatgtggcg
     8761 aaaatcccac tacaaaaagc gactaaaaac ggcattaaac ttgcagcttg gcacgcaaca
     8821 ccggagccgg gtgcgattga taaatacggc attttctaca acgtgacctc tgactcagac
     8881 gaaattgcac gcttatcggc aatgcttgcg gtggcagaat ctaacggtaa ggcaaaagcg
     8941 attattttaa ccgactcgct atatgaaatt gcgttacgca aagcgaacgt catgaaagaa
     9001 gttatcgaaa aatgtaaaga gtgtaaggtg ttagagttta tcgacacgcc attggcggat
     9061 acctcaagcc gtatgcctaa cctcactttc agcttactgc aaaaatatgg cgacgattta
     9121 gagtatgcgt tggcaattaa cgatctctac tttgacttta tggctccgtc tttacgcacc
     9181 gctggtaaag ataaaccgtt tagcatttcc gcaggtgatg gctcagtaac ggcttatcaa
     9241 cgtattcgca atggtgataa acagtttgca accgtccctg agccacttaa cttacacggc
     9301 tggcagttag tcgatgagct aaaccgtgct ttcgctaacg aaaaaccgtc tggttatatc
     9361 acaccggctc acctagtgac taaagaaaat gtggcgtttg acggtggtga aaacaacctg
     9421 tatgccccac aaaacggtta caaaggcgct tacaaagcga tttggggcgt gaagtaatga
     9481 cgtaggggca aataaatatt tgcctcgaac aagcggttgt tttttgcaaa gattttgcga
     9541 aatttaaccg cttgtcataa aaaatagaga gggtaataca aatgagcaat aaatttatcc
     9601 ccaaaatctc caaccctcgt tttaatgagc tggttgggaa tacgtttgaa cttgaagagc
     9661 tttttgataa ggcaatctgg gcagaagggc cggcgtggag caagccacaa caagccctct
     9721 attttagtga tgtaaaaggt tgcacaatgt accgctggac agagcaagat ggtacacagg
     9781 ttttccgcca gccgtcttat ttttctaacg gcaatgcgtt tgatgcaaac gacaacttga
     9841 ttacctgtga acacggcaat cgaggtatca gtttaaccac gccaagcggt gaatatcgtt
     9901 ttttggtcgg tcaattagac ggaaaacgac tgaactcacc caatgatgtg gtggtaaaat
     9961 cagacggcat catttggttc actgatccac cttacggtat tttgtcggat aacgaaggca
    10021 aacagtcgcc aagcgaaatt atcggttgtt atatttactg ctatgatccg aaaaccgaca
    10081 agctgaatat tgcaactttc aacacgatgc gaccaaacgg cttggccttc tcgtcagacg
    10141 aaaaacaact ctttgtggca gatatgtcga gcgtggaatt tgacgaagat ggcttacacc
    10201 acttagtcgt atttgatgtg gacggtaata aactaaaaaa tcgccgagat gtggcggaaa
    10261 ttagtcccgg cattccggac ggcttccgaa ttaaacaaaa cggcgtaatt ttctgctctt
    10321 gtgaagaggg aattattgct attttacctg acggcacgga gctgggacga tttgtgctgg
    10381 gtaagctaac ctcaaattgt agctttggcg atgatgaaca aacactgttt attacttgtt
    10441 cgaatagcgt ttatcgttta acaattaaat agcagaatgc attaagtgcg tctgttgtag
    10501 cattacaagc ggtttctttt tcaaaatttt ttacaaacaa ataatggata acaaaatgaa
    10561 ctacacactc ggcattgatt gcggtgggac ttttatcaaa gccgctcttt ttgatacaca
    10621 aggtaatatt caagcccttc atcgtgaaaa cgtttcggta atcagtgaac aggcaggtta
    10681 tgcagagcgt gatatgtcac aactctgggc tatttgtgcg gaagtagtgc gttcaaccat
    10741 tgaaaaaagt ggggtaaacc cagcggatat taaagcagta gggatttccg ctcaaggcaa
    10801 aggggcgttt ttactggata agcagaatcg accgttgggg cgtgcgattt tatcttccga
    10861 ccaacgttcc cttgagattg ttaaacaatg gcaggctgaa ggcattccgc aacaacttta
    10921 cccgattacc cgccaaactc tttggacagg acaccctgtg tcgattttga agtgggtgca
    10981 gcaaaatgaa ccggagcgtt atgcacaaat cggggcggtg ttgatgtcgc acgactattt
    11041 gcgtttttgc ttaaccggtg agttgcattg cgaagagacc aacatttccg aaagcaatct
    11101 ttacaatatg gaaaaaggcg aatacgacca acagttagcc gatttgttgg gaatgccgga
    11161 tgtgttggag aaattaccac cagtgattga accgaataaa atttcgggat atgtgactga
    11221 aaatgccgcc aaattaaccg gtttagcggt gggtacgccg atagtcggtg gcttgtttga
    11281 tgtggtttct accgcacgtt gtgccaattt agatgacgaa accaaactga atgcagtgct
    11341 gggaacgtgg tcggtggtga gcggcattac cgatcatatt gatgcaagcc aatcattacc
    11401 ttttgtttat gggcgttatg ccgaggcagg gaaatttatc gtccacgaag caagcccgac
    11461 ttctgccggt aatttagagt ggtttgtgaa tcagtggaaa ctcgattatg tgaaaatcaa
    11521 ccaagctgtt gcccaattac cgccggcggc aagctcggtg ctgtttgtgc cgtttctgta
    11581 tggctcaaat gcaggcttag gaatgcaagc cggtttctat ggtatgcaag cccaccatac
    11641 ccaagcccat ctcttgcagg caatttacga aggagtgttg tttagcttga tgagccattt
    11701 aaaccgtatg tttatccgct ttccaaaggc tgacgtgctg cgagttaccg gcggaccggt
    11761 gaaatctaaa gtttggatgc aaatgttggc cgatttaacc ggaatgcggc ttgaaatacc
    11821 acaaatggaa gaaaccggct gcttgggtgc ggcattaatg gcaatgcaag gggttgggct
    11881 ggatataagc caagttcaag ccctaaataa tgaaatgcag gtgtttgagc caaatccgga
    11941 aaattatgcg gcttatcaga cgaaatatca gcgttaccaa aaattaactg aagcattaaa
    12001 tacaatgatt taacctgaat ttatgctttt acaagcggta ata
//
LOCUS       DANLXS010000067        12015 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.067, whole genome shotgun sequence.
ACCESSION   DANLXS010000067 DANLXS010000000
VERSION     DANLXS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12015
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.067"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(6..82)
                     /locus_tag="PXD71_001333"
     tRNA            complement(6..82)
                     /locus_tag="PXD71_001333"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Pro,seq:tgg)
     gene            complement(107..182)
                     /locus_tag="PXD71_001334"
     tRNA            complement(107..182)
                     /locus_tag="PXD71_001334"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(147..149),aa:His,seq:gtg)
     gene            complement(212..288)
                     /locus_tag="PXD71_001335"
     tRNA            complement(212..288)
                     /locus_tag="PXD71_001335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(252..254),aa:Arg,seq:ccg)
     gene            complement(447..1169)
                     /gene="rffM"
                     /locus_tag="PXD71_001336"
     CDS             complement(447..1169)
                     /gene="rffM"
                     /locus_tag="PXD71_001336"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDL6291456.1"
                     /translation="MFDKVIIRGIALQAVKNREIFANWLMNEKRIQFGRLVAINAEKV
                     ILSEKNPLLRRVIDEAEFNYADGISVVQSIRKKYPNYKIERIAGADLWETLMQRAGAL
                     NVPVFLVGSSADTLTKVEAKLAQWKVNIVGRQNGYFKAEQEQDVIEQIKASGAKFISV
                     AMGTPKQELFIQQLQQQYPQALYMGVGGTYDVFAGKVKRAPKIWQNLGLEWLYRLLKQ
                     PTRWQRQLNLLKYAYYYLTNQL"
     gene            complement(1159..2430)
                     /locus_tag="PXD71_001337"
     CDS             complement(1159..2430)
                     /locus_tag="PXD71_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WzyE family oligosaccharide polymerase"
                     /protein_id="HDL6291457.1"
                     /translation="MNNLIPLGGFYLVSVGLFFSLLYQLYQKSGISFHLLFSLLYFVT
                     FYAGFPFSLIMAFGFDYPLPELKTQWQVFTAAGVGYLIYYVVYSRLWSAVSQPTALVA
                     NFQAKTTACILAAVAVVALLYFLYLNGFLLFKLEKYSQLFAKDRVILSALKRFFYFLL
                     PALLIFCFLYKNKTAWWALLIVGGAFGILSYVAVGGTWANLALAVGFFLLIGLQQGYL
                     SLSKVIAVSLIGVVAMFFLALARYKLTLSGSEMIYTFLYLSRDTFSPWENLARVFSNE
                     VEYQGLMPIVRDFYVYIPQSLWQERPEIVWNTANYFTKVILGNQSGLAISPTLLGSFY
                     IMGGFSVMALGMGIVAAMIKALDNLFAYAKACNSALLQAYCFGNLFNLIVLVREGIDA
                     FVSRFCFFTLIFMACYLLAKILSMGKNNNVR"
     gene            complement(2423..3493)
                     /locus_tag="PXD71_001338"
     CDS             complement(2423..3493)
                     /locus_tag="PXD71_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDL6291458.1"
                     /translation="MLPIYHILGSEIEHHNQQVLTFFQQELLPLLPDSEHFFYVVGKP
                     SLQTAFPHLRIKQFSSQKAIANALIAKKRQNPTACFLLHGQFNPWIWLAIALGRLPTT
                     NLIWHIWGADLYEMATSWKFKLFYPFRRVAQQKILQVWATRGDLDHLWRNMRTRSEQD
                     HLIYFPTKLGDQIKISPTQNDRLTILLGNSGDPANELIQALSEIHQRLGNNVRIIIPM
                     GYPENNESYIRQVEAYSKRLFLAENLQILRKKLSFEEYVTLLSHCDLGYFNFKRQQGI
                     GTISLLISHNVPCVLHSANPFGLDLQAEKVPYLLAENLTASQIQTTKQQLKSIDKNRI
                     AFFAPGYTKLWQQRLIELMQNE"
     gene            3680..5101
                     /gene="glnA"
                     /locus_tag="PXD71_001339"
     CDS             3680..5101
                     /gene="glnA"
                     /locus_tag="PXD71_001339"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL6291459.1"
                     /translation="MSNAIANVSKLIQDNDIKFVLLKFTDIKGKEHGVSLPVSLVAND
                     LEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVVDPFARIPTLSIRCSIYDPNT
                     MQSYDRDPRSIATRAENYLKSTGIADSVMFGPEPEFFLFDDVRFSTEMNNVSYRIDDV
                     EAAWNTNRKFEEGNKAYRPLKKGGYCAVAPIDTAHDIRSEMCLLMEEMGLVIEAHHHE
                     VATAGQNEIATRFNSLTLKADETQIYKYIVQNVAVEHGKTACFMPKPFAGDNGSGMHC
                     NMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPTTNSYKRLVPGFEA
                     PVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPMANPYLCFAALLMAGLDGVINKI
                     HPGDAMDKNLYDLPPEELKDIPTVASSLESALDALSNDFEFLTQGGVFTKDFIDAYIG
                     IRRKEVERVNMTPHPVEFELYYS"
     gene            complement(5145..6288)
                     /gene="rffA"
                     /locus_tag="PXD71_001340"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /pseudo
     CDS             complement(5145..6288)
                     /gene="rffA"
                     /locus_tag="PXD71_001340"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
     gene            complement(6275..6919)
                     /gene="rffC"
                     /locus_tag="PXD71_001341"
                     /gene_synonym="wecD"
     CDS             complement(6275..6919)
                     /gene="rffC"
                     /locus_tag="PXD71_001341"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDL6291460.1"
                     /translation="MKPDQWLSDFFGREIVQVKVAADDFRQIARLQKQGFVLVEGELE
                     FELALATFSENMTACRPADMADLPDLNLLFGSAFPDSRFRSPYFSREENQRFYRTWIE
                     NAVKGKFDDIGLVQCNADGQIQGAVTVRLLAQQQAKIGLLAVKPDCRQQGIGSELLNA
                     AIAWAKQQNAKKLMITTQLSNQKAIRFYQAFGASIKAVYYWFYPALEKYDDEQS"
     gene            complement(6916..8163)
                     /gene="wecC"
                     /locus_tag="PXD71_001342"
     CDS             complement(6916..8163)
                     /gene="wecC"
                     /locus_tag="PXD71_001342"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL6291461.1"
                     /translation="MMSKTEFNTICILGLGYIGLPTSVVFANAGKQVIGVDINPQVVA
                     SLNQGNIHIIEPDLQTAFTQAVKKGNFFATAKPQPADAFIIAVPTPLCGKQPDLTYIQ
                     NAAAMIAPCLRKGNLVVLESTSPVGTTENLAEWLSQARPDLAFPADVSIAYCPERVLP
                     GNIMTELVENDRIIGGLSEQCSQQAVDLYQIFAKGRCVTTTARTAEMCKLVENSFRDV
                     NIAFANEISMICDALKINVWELIELANLHPRVNILQPGAGVGGHCLAVDPWFIVSTSP
                     QQARLIRTAREVNDSKPQWVIEQVKTSVADCAMEQDCKPSELTIACLGLAFKPNIDDL
                     RESPALAITRQLAAWHQGSLLAVEPNIQQLAGEKITLVDLNTALSQADILVLLVDHTS
                     FKQISREQIRQRHIIDCRGVWVR"
     gene            complement(8204..9016)
                     /locus_tag="PXD71_001343"
                     /pseudo
     CDS             complement(8204..9016)
                     /locus_tag="PXD71_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS chain length-determining protein"
     gene            complement(9027..10091)
                     /gene="wecA"
                     /locus_tag="PXD71_001344"
     CDS             complement(9027..10091)
                     /gene="wecA"
                     /locus_tag="PXD71_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDL6291462.1"
                     /translation="MWLTFLTVFIVSFLALIVMRPVAEFVGLVDKPNFRKRHQGLIPL
                     IGGIALFIGNLTFYFMQWQDMRLPELYLTAVTILLIIGVLDDRFDISPLLRAGIQAAL
                     AGAMIYSGLSLSSLGQIIAPFSIELGWLGIVFTVFITIGVINAFNMVDGIDGLLAGLS
                     SVSFAGLGTLMFINGEPSLGYWCFALIFVLLPYAMFNLSLFGAKWKVFMGDSGSMLIG
                     FTIIWILLLSTQGQGSPISPITGLWLIAVPLIDMVAVIIRRLKKGKSPFKPDRLHLHH
                     LMMRAGLTSRQALLVITLWAGFCSTIGVLGEVYYWNQWVMMLMFIGLFFLYAYSITHA
                     WRVTRFVRRLKRRAKRSKQL"
     gene            10233..11513
                     /gene="hemL"
                     /locus_tag="PXD71_001345"
     CDS             10233..11513
                     /gene="hemL"
                     /locus_tag="PXD71_001345"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL6291463.1"
                     /translation="MSISEQLFEQAQKVIPGGVNSPVRAFKGVGGTPVFIEKAEGAYI
                     TDSDGKQYIDYVGSWGPMVLGHNHPAIIDAVLKAVPKGLSFGAPTAAEITLAELVCKL
                     VPSIEMVRMVSSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHSDSLLVKAGSGALTL
                     GQPSGPGVPADFAKHTLTCTYNDLDSVKAAFEQYPNEIACLIVEPVAGNMNCIPPQEG
                     FLQGLRDLCTQYGAVFIIDEVMTGFRVALGGAQSYYGVTPDLTTLGKIIGGGMPVGAF
                     GGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGNETILAEKTKTLAEG
                     FKALADKHNVPLTVQYVGGMFGLFFTEQKAVNNFQEVMKCDAAKFNRFFHLMLEQGVY
                     LAPSAFEAGFMSLAHSDEDIARTLEAADKAFAQL"
BASE COUNT         3549 a         2791 c         2426 g         3249 t
ORIGIN      
        1 taaattggtc ggcgagatag gatttgaacc tacgacccac tggtcccaaa ccagttgcgc
       61 taccaagctg cgctactcgc cgtctgatac tcacaagagt attgaatggg gtggctaatg
      121 ggattcgaac ccacgacaac tggaatcaca atccagggct ctaccaactg agctatagcc
      181 accattgtgt tgatagtgcc gcattgacat ctggcgcgct cgacaagatt cgaacttgcg
      241 acctttggct ccggaggcca acgctctatc caactgagct acgaacgctt cgatttacat
      301 caattcaact taatgcgtcg caacggaggc gtatattata gatttcactt tcccttgtct
      361 agcacttttt aaaaaaaatg taaaaaaatg tattgtttgg ttttatttta ttcaaaacgt
      421 ttaataaata tcaaaaaatg cgttttttag agctgatttg tcagatagta ataggcatat
      481 tttagtaaat ttaattgtct ttgccagcgg gttggctgtt tcaatagtcg ataaagccat
      541 tccagcccca aattttgcca aattttaggt gctcgtttca ctttaccggc aaacacatcg
      601 taagtcccgc caacacccat atataaggct tgtggatatt gttgttgtaa ctgttggata
      661 aaaagttcct gttttggagt tcccattgcc acactaataa acttcgcgcc acttgcttta
      721 atttgctcta taacgtcctg ttcttgctcc gccttaaaat agccattctg cctaccgaca
      781 atattcacct tccattgtgc caattttgct tccaccttcg ttagggtatc cgccgagctg
      841 ccgaccaaaa acaccggcac atttaacgct ccggctcttt gcattaaggt ttcccataaa
      901 tcggctccgg caattcgctc tattttatag tttggatatt tttttcgaat cgattgaaca
      961 acactgatgc cgtctgcata gttgaactcg gcttcatcta tcactcggcg taacaacgga
     1021 tttttttccg acagaatcac tttctcagca ttaattgcca ctaatcttcc gaactgaatc
     1081 cgcttttcat tcatcagcca atttgcaaaa atttcccgat ttttgaccgc ttgtaacgca
     1141 atgccccgaa tgatcacttt atcgaacatt attatttttc cccattgaaa ggattttcgc
     1201 cagcaaatag caagccataa atatcagcgt gaaaaagcaa aaacgggaaa caaatgcatc
     1261 gattccctct cgcactaata cgatcaaatt gaataaattg ccgaagcagt aggcttgcaa
     1321 caaggcggaa ttgcacgctt tggcgtaagc gaataggtta tctaaggctt taatcatggc
     1381 agctactatc cccattccca acgccataac gctaaatccg cccataatat agaaagagcc
     1441 gagtaaggta ggcgaaatag ctaaccctga ttggttgcct aaaatcactt tggtaaaata
     1501 attcgccgta ttccagacga tctcagggcg ttcttgccat aaagattgtg ggatatatac
     1561 atagaaatcc cgcacaatcg gcattaaacc ttgatattcc acctcattag aaaatacacg
     1621 agccaaattt tcccaaggcg aaaaagtatc tcggcttaaa tataagaagg tgtaaatcat
     1681 ctcgctaccg gaaagggtaa gtttgtaacg ggctagagcg aggaagaaca tcgccaccac
     1741 gccgattagg cttacggcaa tcacctttga taacgacaga tagccttgct gcaatccaat
     1801 caataagaaa aagccaaccg ctagtgctaa atttgcccat gtgccgccca ctgccacata
     1861 gcttaatatg ccgaatgccc caccaacaat cagtaacgcc caccacgctg ttttattctt
     1921 atataggaag caaaaaatta acaacgccgg caataaaaag tagaaaaatc gttttaatgc
     1981 cgaaagtatg accctatctt tggcaaacag ttggctatat ttttcgagtt tgaaaagcag
     2041 aaaaccgttt aaataaagga aatatagcag ggcaacaact gccacagcgg caaggataca
     2101 agcggtcgtt tttgcctgaa aatttgcaac aagagcagtt ggttgcgaca cagcactcca
     2161 caagcgtgaa tagacaacgt aataaatcaa atagccaaca cctgctgccg taaaaacttg
     2221 ccattgtgtt tttagctccg gcagcgggta atcaaagcca aatgccatta ttaacgaaaa
     2281 cggaaaaccg gcgtaaaaag taacaaaata gagcaaacta aacagtaaat ggaaagaaat
     2341 ccccgatttc tgataaagtt gataaagtaa gctgaaaaat agcccgacag aaacaagata
     2401 aaatccgcca agtggtatta aattattcat tttgcattaa ttcgattaat cgctgttgcc
     2461 atagtttagt gtagcccgga gcaaaaaacg caatgcggtt tttatctatg ctctttagtt
     2521 gttgtttggt tgtttgaatc tgtgaagcag tcagattttc cgccaacaaa taaggcactt
     2581 tttctgcctg tagatccaac ccaaacggat tggctgaatg taacacacaa ggtacattat
     2641 ggctaatcaa aaggcttatc gtgccaatgc cttgctggcg tttaaaattg aaatagccga
     2701 gatcacaatg gctcaataac gtaacatact cttcaaagct cagcttcttc cgcaaaattt
     2761 gcaaattttc cgccaaaaat aaccgcttgc tataggcttc cacctgccga atatagcttt
     2821 cattattttc cgggtagccc attggtatga taatccgcac attgttccct agtcgttgat
     2881 gaatctcgct taaggcttga ataagctcat tcgctggatc gcctgaattg ccgagcaata
     2941 tcgtcaagcg atcattttgt gtggggctaa tttttatttg atcacctaat ttcgtgggga
     3001 aatagattaa atgatcctgt tctgatcttg tacgcatatt tcgccacaaa tgatccagat
     3061 cgcctcttgt tgcccaaacc tgtaagattt tttgttgggc aacacgccga aacgggtaaa
     3121 acagcttaaa tttccacgaa gttgccattt catataaatc cgccccccaa atatgccaaa
     3181 tcaggttggt ggtcggcaat ctgcctaagg ctatcgccag ccaaatccaa gggttaaact
     3241 gcccgtgtaa cagaaaacaa gcggtcggat tttgccgttt ttttgcaatt aaggcgttcg
     3301 caatcgcttt ttgtgaagag aactgcttaa tccgcaaatg aggaaaagca gtttgcagtg
     3361 aaggcttgcc taccacataa aaaaaatgtt cggagtctgg caataacggc aaaagctctt
     3421 gctgaaaaaa ggttaaaacc tgttggttat gatgctcaat ctcggagcct agaatatgat
     3481 aaatcggcaa cattacacgc ttttctctcc ccaccataca aacaatatgc aagcggtaag
     3541 attttagcaa aaatttgcaa cattactgca acttctttta gctttgaaac actaaaaaac
     3601 atcacacact tggttaaaaa atgctatgat aaccgagctt ttttattatt catttatccc
     3661 aacgaggact tattttacta tgtcaaacgc aatcgctaat gtatctaaac tgattcaaga
     3721 caatgatatt aaatttgtct tattaaaatt tactgatatt aaaggtaaag aacacggggt
     3781 ttcgctccct gtcagcttag ttgccaacga tttggaagac ttattcgagg aaggcaaaat
     3841 gttcgacggt tcgtccgtag aaggttggaa ggcaattaac aaagcagata tgttgttaat
     3901 gccgattgct gaaaccgctg tagttgaccc tttcgcccgt attcctaccc tttctattcg
     3961 ttgttccatt tacgacccaa acacaatgca gtcttatgac cgtgaccctc gctctattgc
     4021 aacccgtgcc gaaaactact tgaaatccac cggtatcgcc gatagcgtaa tgtttggacc
     4081 tgagcctgaa tttttcttat tcgatgatgt gcgttttagc accgaaatga acaatgtgtc
     4141 ataccgaatt gacgatgtag aagcggcttg gaataccaac cgtaaatttg aagaaggcaa
     4201 caaagcctat cgtccgttga aaaaaggcgg ctactgtgcg gttgcaccga ttgataccgc
     4261 ccacgatatt cgctccgaaa tgtgtttatt gatggaagag atgggtttag tgattgaagc
     4321 tcaccaccac gaagttgcga cagccggaca aaatgagatc gcaacccgat tcaacagcct
     4381 gaccttaaaa gccgatgaaa cccaaatcta taaatacatc gtgcaaaatg ttgccgtaga
     4441 acacggaaaa accgcttgct ttatgccaaa accgttcgcc ggcgacaacg gctcgggaat
     4501 gcactgtaat atgtcattaa gcaaagacgg caaaaatgtg tttatgggcg ataaatacgc
     4561 cggcttatct gaaactgccc tctactatat tggcggtatc attaaacacg caaaagcatt
     4621 aaatgccttt actaacccaa ccacgaactc ttataaacgt ttagtgccgg gttttgaagc
     4681 accggtgtta ttggcatatt ctgcaagtaa ccgctctgcc tctatccgta ttccggctgt
     4741 gaccagtccg aaagcgaccc gtgtagaagc acgcttcccg gatccaatgg caaacccata
     4801 cctttgcttt gccgccctgt taatggctgg cttagacggt gtgatcaaca aaatccaccc
     4861 gggcgatgca atggataaaa acttatacga tttaccgccg gaagagttaa aagatattcc
     4921 aacggttgcc tcgtctttag aatccgctct tgacgcatta agcaatgatt ttgaattctt
     4981 aacacaaggt ggcgtgttca cgaaagactt tattgatgct tatatcggta ttcgtcgcaa
     5041 agaggtggaa cgtgtaaata tgactccgca cccggtagaa tttgaacttt actattcata
     5101 gtaacaacat atagcaaaaa acggctgaaa cactcagccg ttttttattg attgaagaaa
     5161 gcaagcactg cttgaataac acgctcttgc tcctgcttac tcaaattata aaacagtggc
     5221 aatcttacca accgttcgct ttctaaggta gtaaagcgat catcacctgc taatcgacct
     5281 aatttctgcc ctgccggact gctgtgcaac ggcacataat gaaacacggt taagatattc
     5341 tgtgccttca taaaatcaat aaaagcagtt ctctgcttca agtcacttag ctttaagtag
     5401 aacaaatgcc cgttatggcg acaatattcg ccaatgcgag gaagctcgca cttagctgtt
     5461 tgggcaagtg gcaaaaaggc ttgatagtaa ttgtgccaaa gggttaaacg ctgttggttg
     5521 attttatctg cctctataag ctgagcatac aaataagccg cttgaatttc cgacattaaa
     5581 aaacttgaac caatatcctg ccagcgatat ttatctacct caccacgaaa aaattggcta
     5641 cggttagtgc ctttctctct gatgatttcc gctttttcca gcaaatgcct cattaatcac
     5701 gatcgcccca ccttcaccgc cagcggtata gtttttggtt tcgtgaaaac tatagcaacc
     5761 aatatgcccg atggaaccta acgccctacc tttataaaac gaattgactg cctgtgccgc
     5821 atcttctacc acataaagcc cgtatttttc ggctgttgcc attattgaat ccatatcaca
     5881 agcaatgccg gcataatgca ccggcacaat cactttggtt tttggggtaa tggcttgctc
     5941 aattttgcgt tcatcaatat tcattgtatc cggacgaata tccacaaaca caatctttgc
     6001 cccacgcaac gcaaacgcat ttgcagtaga gacaaaagta tagctcggca taatcacttc
     6061 atcgtccggc tgaatatcca gcagtattgc cgccatctct aacgcagccg tgcaagaagg
     6121 ggtaagcaac actttgctac gggccaggtt ttcaagccac tgctcacata agcgggtgaa
     6181 tttaccaccg cctgaaagct gccctgttgc catcgcctgt tctatatagg cttgctccgc
     6241 ccccacaacc gggggttgat taaacggaat aaaatcagga ttgttcatca tcgtattttt
     6301 ccaaagcagg gtaaaaccaa taatacaccg ccttaatgct tgcaccaaac gcttgataaa
     6361 aacgaatcgc tttctggtta ctcagctgag tggtaatcat cagttttttc gcattttgct
     6421 gtttcgccca cgctatcgca gcatttaaca attccgagcc aataccttgt tgtcggcaat
     6481 caggctttac cgccaataat ccgattttcg cttgctgttg ggcaagtaag cgaaccgtta
     6541 ccgccccttg aatttgcccg tcagcattgc attgaaccaa gccaatatca tcaaacttgc
     6601 cttttaccgc attttcaatc caagtccggt aaaagcgttg attctcttcc cttgaaaaat
     6661 agggcgaacg aaaccggcta tcgggaaatg ccgaaccaaa caataagttc aagtcgggca
     6721 aatctgccat atccgccggg cgacaagcgg tcatattttc agaaaatgtt gcaagtgcca
     6781 gctcaaattc caattctccc tccacaagta caaaaccttg tttttgcaac ctagcgattt
     6841 gcctaaaatc atcagccgct acttttactt gcacaatttc tctaccgaaa aaatcggaca
     6901 gccattgatc cggcttcatc ttacccacac tcctcgacaa tcaataatat gccgttgcct
     6961 gatctgttcc cttgaaattt gcttaaatga agtgtgatcc actaacaaga ctaaaatgtc
     7021 tgcctgcgaa agtgcggtgt tgagatcgac taaggtgatt ttttcgccag ccagctgttg
     7081 aatatttggc tcaaccgcca acaggctacc ctgatgccaa gcggcaagtt gccgggtaat
     7141 cgctaatgcc gggctttccc gcaaatcatc aatattcggc ttgaaagcta gccctaaaca
     7201 agcgatggtt agctcacttg gcttacaatc ttgctccatc gcacagtctg cgaccgatgt
     7261 tttcacttgc tcgattaccc attgcggttt gctgtcattc acttctcgtg ccgtgcgaat
     7321 aaggcgagcc tgttggggcg atgtggaaac gataaaccac gggtcaaccg ccaaacaatg
     7381 tccgcccacc cctgctccgg gctggagaat atttactctc gggtgaagat ttgccaattc
     7441 aattagctcc cacacattga tcttcaacgc atcacaaatc attgaaattt cattggcaaa
     7501 ggcaatgttc acatcacgaa agctgttttc caccaacttg cacatctccg ctgtgcgagc
     7561 tgtggtggtg acacaacgtc cttttgcaaa aatttggtat aaatcgaccg cttgttgact
     7621 gcattgttcg cttaatccgc cgataatgcg gtcattttca accaattccg tcattatatt
     7681 acccggcaaa acacgttcag ggcagtaggc aatagacaca tcggctggaa aggctaaatc
     7741 agggcgagct tggcttagcc attcggctaa attttctgtt gtaccgacag gcgaagtgga
     7801 ctccagcacc accaaattcc ctttacgcaa acaaggggca atcatagcgg cggcattttg
     7861 aatataagtg agatcgggct gcttcccgca aagtggggtt ggcacggcga tgataaaagc
     7921 atcggccggt tgcggtttgg cagttgcaaa aaaattacct tttttgaccg cttgtgtgaa
     7981 ggcagtttgt aaatccggct cgataatatg aatgttgcct tgattaaggc ttgccaccac
     8041 ttgcggatta atatccaccc caattacttg cttgccggca ttggcaaaaa cgactgaggt
     8101 cggcaagccg atatagccta accccagaat acaaatagtg ttaaattcag ttttactcat
     8161 catcagttac aaagagaaat ggcacactaa attgtgtgcc attttagatt aaagttccgc
     8221 aggttgagct ttcgctttca ccgcaaaact gagcataaag cccagcagta agccacttaa
     8281 tgccccaatc atcagccaaa ataggcggat caggcgattc tgatttaagt ggaacgctag
     8341 gcgatttaac caagcgataa gctgtaagtt ggttgtctaa cggctgaaca gaacgcatca
     8401 tttgcagttt agctgcccaa tcttgtgagc cttgcttacc gttaatattc aactccgaag
     8461 cagttttaat ttgctggaac aagactttcc atttcgcaat taaatcggcg tttaatttat
     8521 ctcgagctta ggtgtttacc aacggaataa aatcggctaa taatttttga gcctcttccg
     8581 cattttctgc aattaagctg acattatctg ccgaagttgc catatctttt tcaaaacgga
     8641 attgttcaac taattcagcc gccaccaccg ccggtgattt ctttgcaacc tgagctttta
     8701 atttcaccgc ttctgtttgt agcaaatagg cttgaagcaa gtcggcggac gtgaggttgc
     8761 gtttaaattc cgcataaacc tgctcacttg cctgttgctg tgccgtttgg ctattcgctt
     8821 cttggctatt actattcaaa aaatggtagg tggaaagtaa cgagtaataa ttacctaaat
     8881 cgggaatagt cggttgctca aattgagcgg tcactttcca tttttcaggt tgagtataac
     8941 tcagcccata accggcacct gccccgatag cagcaaataa aattagcact aaaagtcgat
     9001 aaaataaggt tttcacaaca tatcctttaa agttgtttgc tacgttttgc acggcgtttt
     9061 agacggcgaa caaaacgggt tacacgccaa gcgtgggtaa tggaataggc gtataggaaa
     9121 aacagcccga taaacattag catcatcacc cactgattcc aataatacac ctcgcctaat
     9181 accccaatgg tggaacaaaa tcccgcccag agggtaatca ccaataatgc ttggcgagag
     9241 gtaagccctg ctcgcatcat taaatggtgt aggtgcaagc ggtctggttt gaacggactt
     9301 ttaccttttt tcaaacggcg gataatcacc gcaaccatat caattaacgg cacggcaatc
     9361 agccacaaac cggtaatcgg gctaattggc gagccttgcc cttgggtgct taacagcaga
     9421 atccaaataa tggtaaagcc gatcagcata ctgcctgagt cgcccataaa cactttccat
     9481 ttcgccccaa acaggctcaa attgaacatt gcatacggca acaacacaaa gataagggca
     9541 aagcaccaat agcccaaact tggttcgcca ttaatgaaca ttaaagtgcc aagaccggca
     9601 aaactcacgc tggaaagccc tgctaataag ccatcaatcc catctaccat attaaacgca
     9661 ttaataacgc caatggtgat aaacacggta aagacaatgc cgagccaacc taattctata
     9721 ctaaaggggg caataatttg ccctaaactg ctgagcgaca agccactgta aatcatcgcc
     9781 ccagctagtg ctgcttgaat cccggcacgc agtaatgggc tgatgtcaaa tcggtcatct
     9841 aacacgccga taatcagcaa aatagttacc gctgtcagat acagttccgg cagccgcata
     9901 tcttgccact gcataaaata gaaagtgaga ttgccgataa ataatgcaat accaccgatt
     9961 aacggaatca acccctgatg gcgtttacgg aagttgggct tatctaccaa accaacaaat
    10021 tccgccaccg gacgcatcac aatcagggct aaaaaagaaa caataaatac ggttaaaaaa
    10081 gtaagccaca tacagggatt gcctcaaaaa atgagtcata aaataggaat gcagaatagc
    10141 ataaggcaac aaaatcgtaa attttttagc gaaaacaagg gcttttcagt acaataacag
    10201 gcaaattttt acatcaaaag aaaggaattg atatgtctat ttcagaacaa ctgtttgaac
    10261 aagcccaaaa agtgattccg ggcggtgtga actcccctgt gcgtgcattt aaaggcgtgg
    10321 gcggcacacc ggtttttatt gaaaaagccg aaggtgctta tattaccgac agcgacggca
    10381 aacaatatat cgactatgtc ggctcttggg ggccaatggt actcgggcat aaccacccgg
    10441 cgattattga tgcggtgcta aaagccgtgc caaaaggctt aagtttcggt gccccaacgg
    10501 cagcagaaat cacacttgcc gaattagtgt gcaaattagt accttctatt gaaatggtga
    10561 gaatggtgag cagcggaaca gaggcaacaa tgtctgccat tcgtttggct cgtggctata
    10621 ccaaccgaga taaaatcatc aaatttgaag gctgctacca cgggcattcc gattctctgt
    10681 tagtaaaagc cggttcaggt gctttaaccc tcggtcagcc aagcggcccg ggcgtacccg
    10741 ctgatttcgc caagcatacg cttacttgca cctataatga tttagattca gtaaaagcag
    10801 catttgagca atacccaaat gagatcgctt gtttaattgt tgagcctgtt gccggcaata
    10861 tgaactgcat tccgccacaa gaaggctttt tacaaggctt acgtgatctt tgcacacaat
    10921 atggtgcggt atttattatt gatgaagtaa tgaccggctt ccgtgtcgca ctcggtgggg
    10981 ctcaatctta ctatggggta acaccggatt taaccacact cggcaaaatt atcggcggcg
    11041 gaatgccggt gggggcattt ggtggtaaaa aagagattat ggaatacatc gctccaactg
    11101 gcccggttta ccaagccggc acgctctcag gcaacccgat tgcaatggct gcaggtttgg
    11161 catgcttaac tgaattatca aaagcaggaa atgaaaccat tttagcggaa aaaaccaaaa
    11221 ccctcgctga aggctttaaa gctcttgcag ataagcacaa tgtgccatta acggtacaat
    11281 atgtcggcgg aatgttcggc ttattcttca ccgaacaaaa agcagtaaac aacttccaag
    11341 aagtaatgaa atgtgatgcc gccaaattca accgtttctt ccacttaatg ctggaacaag
    11401 gcgtgtattt agccccatcg gcattcgagg cagggtttat gtctttagct cacagtgatg
    11461 aagatattgc tcgtacgcta gaggctgcgg ataaggcatt tgctcagctt tagaagaatg
    11521 acaagcggtt gggtttactc aataatttgc aagtttttga gggaatttga ccgcttgtag
    11581 tgcaaatttc tcttacgata gcaattatga tggtgttcgt ctctgacgag tgcttataca
    11641 aactttcatt atgcttaaac aagcacccgt ctggagacgg gcgctatcga gggaatagga
    11701 ttgataaagc aatatatggg cttccaacac ttgcaggcaa ggacaagtct tacactttct
    11761 aatattacaa atatatttct ttctttaatc tataccataa aggtttaagt tggttcttta
    11821 taactttgga atccatagat gaataagctc tctttccata agagataaaa ttagattttc
    11881 cgaaatgaga atacttagaa aatagctttc ttttataaat gcctttgata gctacccctc
    11941 gtcttattct tatcctatta tatttaccct gagtttgctt agctagacta acccctcgtt
    12001 tcattttatt tgaat
//
LOCUS       DANLXS010000068        11908 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.068, whole genome shotgun sequence.
ACCESSION   DANLXS010000068 DANLXS010000000
VERSION     DANLXS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.068"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..568
                     /locus_tag="PXD71_001346"
     CDS             <1..568
                     /locus_tag="PXD71_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6291464.1"
                     /translation="IAVQYPEEIDIKSKNGKVAKQFCQRKNLPLYITEISKERIRRAG
                     KQILQNNPENRPLDVGFKVFKLTESHFKQWQSPTSENLADQLEMFIDPVSEKADTQAV
                     LYELLLRLGLKLTAKVRLENKVFWVEENGLIFALLLNAADEEIIQTVIAQQPKKVIAL
                     DRLFNGNDARKKNTELQMQDAGITFFVI"
     gene            578..3214
                     /locus_tag="PXD71_001347"
     CDS             578..3214
                     /locus_tag="PXD71_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL6291465.1"
                     /translation="MELQFETLEYQLQAVNATVNLFVGQPNAATEFSLKAQNDMRFVP
                     NLGLQISDEQLQQNLANLQNRQKIDRTLLVEQGKNFTVEMETGTGKTYVYLRTIFELN
                     RQYGWQKFVIVVPSVAIREGVLHTLETTKSHFNTVFDNPSVNQKFEYKSNQTSRLKSF
                     ASANHIEILVMNIDAFTKESNVINTVNESGDAPIFYIQQANPIVIIDEPQNMETEIRR
                     NAIESLSPLFTLRYSATHKNAYNPIFSLNPVQAYEMGLVKQIEVDSVLAENEVNGAYL
                     SLKEIKTGAKSWSAKIEILFNDKKAMKKKVISVKPNQDLYELSNQNEMYRSGFILSGM
                     DTDSQMVSFSNGTQIFQGAEHSQLKEDVQKMQIRRTIGEHLQKEKKLRPLGIKVLSLF
                     FIDKVDNYRPSAQSPTGGKFAKWFEEIYLELAKEEPQGVHNGYFSQDKKGVAKDTNGT
                     TQADNETYQLIMRDKEKLLSLENPLRFIFSHSALKEGWDNPNVFQICTLNDTASEMKK
                     RQEIGRGLRLPVNQQGLRIYDRHLNTLTIIANESYEQFAATLQREIEEDCGVQFAKSN
                     VKKREDKKAVRYRKDFSLHPEFAAIWERIKHQTTYRVDFSNAELIESAVEKIRKMPDI
                     EATKIKHSKVKLEMNETGISTNYRSSNETAVNTQWAIPDLLSEIQKKTGLTRATLCKI
                     LQKSDRLQEVFRNPQRFIDLIAEKINLALQQIMTDGVEYHKIAGKTYEMTLFGDFDFY
                     LNQYTFEVQDKTKTIYEDYIALDSQTENIFARHCESSEQVEFYFKLPKRFKIPTPLGS
                     YNPDWAVVFKGENKIYLVAETKNTGKGIQAGVDEEKLDESERMKIDYARKHFKGLQRD
                     YKDLDYRVVQKVEELSAEYCKI"
     gene            3346..5778
                     /gene="gyrB"
                     /locus_tag="PXD71_001348"
     CDS             3346..5778
                     /gene="gyrB"
                     /locus_tag="PXD71_001348"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL6291466.1"
                     /translation="MSEQTQATSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHM
                     VFEVVDNAIDEALAGHCKDIIVTIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTV
                     LHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLA
                     IIGNTEKTGTSVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIKLIDERDGKE
                     EHFKYEGGIKAYVEYLNEGKTHIHNTPFYLTTEKDGIGVEISLQWNDSYNENVYCFTN
                     NIPQRDGGTHLAGFRGALTRALKNYMDNSGVLKKADANIDASGDDAREGLVAVISVKV
                     PDPKFSSQTKDKLVSSEVRGAVESSMNEALSDYLAENPDDAKNIVTKIIDAARAREAA
                     RKAREMTRRKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAI
                     LPLKGKILNVEKARFDKMLSSQEVATLITALGTGIGRDDYNLEKLRYHKIIIMTDADV
                     DGAHIRTLLLTFFYRQMPELIENGYIYIAQPPLYKVKKGKQERYIQDNDEMSQYEIDI
                     ALEGAGLYVSEDAPALSGVALENLIAQYNGVQKLIERLSRRYPTLLLNELIYSKPLSV
                     EFAKNQANMTAWSEQFVATLMAKEVGGSFYRINTLFNEERQIYEPEIIVTTHGMDSTY
                     RLDYNFINGNEYARIVALGNELNDLLSDSAYVVRGERRQEISSFAEALDWLVKESRKG
                     LTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDF
                     IETNALYAQLDI"
     gene            complement(5831..6793)
                     /gene="cmoB"
                     /locus_tag="PXD71_001349"
     CDS             complement(5831..6793)
                     /gene="cmoB"
                     /locus_tag="PXD71_001349"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDL6291467.1"
                     /translation="MIDFRPFYQQIATTDLSAWLETLPLQLKQWEKTTHADYAKWAKV
                     ADFLPNSTACINLKDKVESISSKPLSEGEVKQITHHLKQLMPWRKGPYHLHGIHIDTE
                     WRSDFKWDRVLPHLAPLKDRTILDVGCGSGYHMWRMVGEGAKMVVGIDPTELFLCQFE
                     AVRKLLGNDRRANLIPLGIEQMQPLAAFDTVFSMGVLYHRKSPLDHLSQLKAQLVRGG
                     ELVLETLVIDGDINDVLVPADRYAKMKNIYFIPSVAALINWLEKVGFKNVRCVDEAIT
                     TLEEQRKTDWLENESLVDFLDPNDHSKTIEGYPAPKRAVILANA"
     gene            complement(6890..8653)
                     /locus_tag="PXD71_001350"
     CDS             complement(6890..8653)
                     /locus_tag="PXD71_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDL6291468.1"
                     /translation="MSEQTFITGKDAALEDSIATFQHKLKTLGFNIEEASWLNPVPNV
                     WSVHIRDADCPQCFSNGKGASKKAALASALGEYFERLSTNYFWADFYLGQEIANSEFV
                     HYPTEKWFPIDDEAIPPMEMMDEFLWEYFDPNGEITPELLVDLQSGNYDRGIVTLPYV
                     RQSDNETVYIPQSIIGNLFVSNGMSAGNTKNEARVQGLSEVFERFVKNRIITEAISLP
                     EIPQSVIDGYPTIKASIEKLEQEDFPILCYDASLGGEFPVICVILLNPNNGTCFASFG
                     AHPNFQVAFERTVTELLQGRSLKDLDVFAPPSFNNEDVADHANLETHFIDSSGLISWD
                     LFKRDADYPFVHWDFSGTTEQEFNNMMAIFNKHNQPVYIMDYEHLGVYACRILAPGMS
                     NIYPSDDLVYANNNMGMDWREILLDLPHFHHDAETYQELLDELDEQSIDDLTRVREFI
                     GVVAEKGSAMQTLRIGELKSMLHLALGNLEQASDWANWTANMNASVFSAERNNYYRCL
                     IQSIELFLDEERDSEQYRMVFEKMYGKQAVEAAWGAIQGGNPFHGLTASDESLENMTA
                     HQKLLNAYRKVQKAKVNPIAR"
     gene            8917..11205
                     /locus_tag="PXD71_001351"
     CDS             8917..11205
                     /locus_tag="PXD71_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDL6291469.1"
                     /translation="MEEHTIQSFYAHSGKLSDHSDWQTLLSHANNVGLLAAEFAAPFA
                     SQEIARCTGLLHDLGKYSERYQKRLHGGERVNHSTAGAKIAVERWGPLGKMMAFCIAG
                     HHAGLANGSGEGKNRSTLTQRLQEQFGYGHKDLPVLDAIWQQELNLPEKLPLPPLKPS
                     KNEPFFSYAFFIRMLYSCLVDADFLDTEAFYANLEKRPLERGNYPNLTALHQQFNQFI
                     AQFRERIATQTPNTQEAQRKAEVNRLRSEILDYAVSQAKQETGLFSLTVPTGGGKTFT
                     SMAFALEHAKQYGLCRIIYVIPFTSIIEQNAAEFRKAFGELGTEAVLEHHSTFDHDSL
                     PDKSSRDKLKLASENWDTPVVVTTAVQFFESLFADRSSRCRKLHNIVGSVIILDEAQM
                     LPLNLLRPIMAAIDELARNYQCSVVLCTATQPAIHQANGFYKGFENVREIAPNPTALF
                     EKLKRTTVKHIGIQTDVELQSKLADNPQMLIIVNNRRHARALYDSAKQLEGAYHLTTL
                     MCAKHRSQTLTEIRLRLKQNLPCRVIATSLIEAGVDVDFPLVMRAEAGLDSVAQAAGR
                     CNREGKRLSVESNVWVFQPEEQWKAPPELGLLSGCMRSTVQVHADDLLNPKAINHYFQ
                     AVYEAKGKELDKPNILKACHDAGQSLNFPFQKIAEDFRMITSHLIPVIIPFDQEAENL
                     IDRLRYADQVGGILRKLQPYTVQVPEKALAELYNAGRVETINETNFGKQFYCLIGMDL
                     YDEVAGLSWENVDFLESEKLVL"
     gene            11336..>11908
                     /locus_tag="PXD71_001352"
     CDS             11336..>11908
                     /locus_tag="PXD71_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291470.1"
                     /translation="MREPLLDEDELKKWLQTIFTDNLVIIVGSGLSCAEGLPGMGALA
                     DRLKERMPECLDDIDKVTWNTISDCLDSEGLEGALLKHQANETIEAAIIKITAEYVLS
                     EEQKAINKCIAENQKLKFSYLLPHISASNPKIARVITTNYDRLIEFAAEYENWGIDSM
                     MVGRYWGKHNPDLSRKLQIRDIRVKGKCPKL"
BASE COUNT         3745 a         2285 c         2564 g         3314 t
ORIGIN      
        1 tattgcggtt caatatcctg aagaaattga tatcaagagt aagaatggaa aagtagcaaa
       61 gcaattttgc caaagaaaaa atttaccact atatataact gaaatttcaa aagaacgtat
      121 tcgccgtgcc ggaaagcaga ttttgcaaaa taaccccgaa aaccgaccgc ttgatgtagg
      181 ttttaaagtc ttcaaactga cagaaagcca ttttaaacaa tggcaatcgc ccacatctga
      241 aaatttagcc gatcaacttg aaatgtttat tgatcctgtt agtgaaaaag cggatacgca
      301 agccgtttta tatgagcttt tattgcgttt agggttaaaa ctcacggcaa aagtgcggtt
      361 ggaaaataag gtgttttggg tggaagaaaa cgggctgatt ttcgccttgc tgttaaatgc
      421 agccgacgaa gaaattattc aaactgttat cgctcaacaa ccgaaaaaag tcatcgcttt
      481 agaccgttta ttcaacggca atgatgcccg gaagaaaaac accgaattgc aaatgcaaga
      541 tgccggcatt acgttttttg tgatttaagg agaaaaaatg gaactccaat ttgaaacctt
      601 agagtatcaa ttacaagccg ttaatgccac agtaaatttg tttgtcggac agccgaatgc
      661 cgcaactgaa ttttcactca aagcccaaaa tgatatgcgg tttgtgccga atttaggctt
      721 gcagattagt gatgagcagt tacaacagaa tttggctaat ctgcaaaaca ggcaaaaaat
      781 cgaccgcact ttgcttgtcg aacagggaaa aaattttacc gtggaaatgg aaactggtac
      841 agggaaaacc tatgtctatt tacgcacgat ttttgaatta aaccgtcaat acggctggca
      901 aaaatttgtg attgtcgtgc cgagtgtggc aattcgtgag ggagtgttgc atacgttgga
      961 aactacaaaa agccatttta atacggtatt tgataacccg agcgtcaatc aaaaatttga
     1021 atataaaagt aaccaaactt cacgcttaaa atcctttgct agtgctaatc atattgaaat
     1081 tttagtgatg aatattgatg ctttcaccaa agaaagtaac gtgattaata ccgtgaatga
     1141 aagcggtgat gctccgattt tctatattca acaggcgaat ccgattgtga ttattgatga
     1201 accgcaaaat atggaaacgg agattcgccg taatgcgatt gagagtttaa gtccgctttt
     1261 cactttgcgt tattctgcca cccataaaaa tgcgtataac ccgattttta gcctcaatcc
     1321 ggtgcaagcc tatgaaatgg gattagttaa acaaattgag gtagatagcg tattggcgga
     1381 aaatgaggta aatggagctt atctttccct caaagagatt aaaacagggg caaaaagctg
     1441 gtcggcaaaa atcgaaattt tatttaacga taaaaaagcg atgaaaaaga aagtgattag
     1501 cgtgaaaccg aatcaggatt tgtatgagct ttccaaccaa aatgaaatgt atcgttcagg
     1561 gtttatatta agcggaatgg ataccgatag ccaaatggtt agcttctcca acggcacaca
     1621 aatttttcaa ggggcggaac attcccaact taaagaagat gtgcaaaaaa tgcagattcg
     1681 ccgcactatt ggcgagcatt tgcaaaaaga aaagaaatta cgaccgcttg ggatcaaggt
     1741 gttgtctttg ttctttatcg ataaagtgga taattatcgc ccttctgcac aaagccctac
     1801 ggggggaaaa tttgcaaaat ggtttgagga aatttatttg gagctagcca aagaagagcc
     1861 tcaaggcgtg cataacggtt atttctcaca ggataaaaaa ggcgtagcaa aagatactaa
     1921 tggcacaacg caagcggata acgagactta tcaactgatt atgcgggata aagaaaaatt
     1981 gctttcgctg gagaatccgt tacggtttat tttctcgcat tctgccttaa aagaaggatg
     2041 ggataatccg aatgtgtttc aaatttgtac tttaaacgat actgcaagcg aaatgaaaaa
     2101 acgccaagag attgggcggg gcttgcgttt gcctgtgaat caacaaggat tgcggattta
     2161 tgatcgtcat ctaaatacgc tgacaattat cgcaaatgaa agttacgaac aatttgccgc
     2221 cacattacag cgtgaaattg aggaagattg tggtgtacag tttgccaaaa gcaatgttaa
     2281 aaaacgggaa gataaaaaag ccgttcgcta ccgtaaagat ttcagcttac atccggaatt
     2341 tgcggcaatt tgggaacgga ttaaacacca aaccacttat cgggtagatt tcagtaatgc
     2401 ggaattgatt gaaagtgcgg tggaaaaaat ccgcaaaatg cccgatatcg aggcgacaaa
     2461 aattaagcat tccaaagtga aattggaaat gaatgaaaca gggatttcaa ctaattatcg
     2521 ctcatctaat gaaacagcgg taaatacaca atgggcgata cctgatttat tgagtgagat
     2581 tcaaaagaaa accgggctga cgcgtgcgac cctttgcaaa attttacaaa aatccgaccg
     2641 cttgcaagaa gtcttccgca atcctcaacg ctttattgat ttaattgcag agaaaatcaa
     2701 ccttgcttta caacaaatta tgacagacgg ggtggaatat cacaaaattg ccggaaaaac
     2761 ctatgaaatg acgttattcg gcgattttga tttttacctc aatcaatata cttttgaagt
     2821 tcaagataaa acaaaaacga tttatgaaga ctatattgca ctggattccc aaacagaaaa
     2881 tatcttcgcc cgacattgtg aatctagtga gcaagtggag ttttatttta aattacccaa
     2941 acgttttaaa attccaacgc cattaggcag ctataatccc gattgggcag ttgtgtttaa
     3001 gggagaaaat aaaatttact tggttgccga aacgaaaaat acaggcaaag gtattcaagc
     3061 aggagtagat gaagaaaaat tagatgaaag tgaacgaatg aaaatcgact atgcccgtaa
     3121 gcatttcaaa ggcttacaga gagattataa ggatttggat tatagggttg tacagaaagt
     3181 ggaggagctg tctgcggagt attgtaaaat ttaatctgtg gtcttgtaga attgagatag
     3241 actgcaattt caactaaaat ttaaccgctt gttttgcgat acttttacgc atttttctgc
     3301 tataatcggc acaatttttt cgatttcaaa ttagtaggaa taagaatgtc tgaacaaact
     3361 caagcaacaa gttatgattc ttccagtatt aaggtgttac gcggcttaga tgcggtgcgt
     3421 aaacgcccgg gaatgtatat cggcgatacc gatgacggta cgggcttaca ccatatggtg
     3481 tttgaggtgg ttgataatgc gattgacgaa gcattagccg ggcattgtaa agacattatt
     3541 gtcacaattc attccgataa ttcggtttct gtgcaagatg acggtcgtgg aattccggtg
     3601 gggattcacc cggaagaagg cgtttcggca gcagaagtca ttatgactgt tcttcacgca
     3661 ggcggtaaat ttgacgataa ctcctataag gtttccggcg gtttgcacgg tgtgggcgta
     3721 tcggtggtga atgcactttc ctctaaatta caactcacta ttcgccgtga aggacacgtt
     3781 cacgagcaat tctatagctt aggtgagcct gatgcacctt tagcgattat cggcaatacc
     3841 gaaaaaacgg ggacgtcggt tcgtttctgg ccaagtttgg atattttcaa aaataaaacc
     3901 gaatttgaat ataaaattct gtctaaacgc ttgcgtgaac tatcattttt gaattcaggc
     3961 gtttcgatta aattaattga tgaacgagac ggcaaagaag agcattttaa atatgagggc
     4021 ggtattaaag cctatgttga gtatttaaac gaaggcaaaa ctcatattca caatacgcca
     4081 ttctatttga ccactgagaa agacggcatt ggcgtggaaa tttccctaca gtggaatgac
     4141 agttacaacg aaaatgtgta ctgctttacc aataatattc cacagcgtga tggcggtacg
     4201 cacttagccg gtttccgtgg ggcattaact cgtgcgttga aaaactatat ggataacagc
     4261 ggggtattga aaaaagccga tgcgaacatt gatgcctcag gtgatgatgc ccgtgaaggt
     4321 ttagtagcgg tgatttcggt gaaagtgccg gatccgaaat tctcctcaca aaccaaagat
     4381 aaattggtgt cgtcagaagt gcgtggagca gtggaaagct caatgaatga ggctttatcc
     4441 gactatttag ccgaaaatcc ggacgatgca aaaaatatcg ttaccaaaat tattgatgca
     4501 gcccgtgcaa gagaagcagc tcgtaaagcc cgtgaaatga cccgccgtaa aggggcgtta
     4561 gatttaggcg gcttaccggg taaattagct gattgtcagg aaaaagatcc ggcgttatcg
     4621 gagctttact tggtggaggg tgactctgcc ggtggctcgg caaaacaagg gcgtgaccgt
     4681 aaaaaccaag cgattttgcc gttaaaaggg aaaattctga acgtggaaaa agcccgcttt
     4741 gataaaatgc tttcttcaca agaagtagca accttgatta ccgctttagg cacaggcatc
     4801 gggcgtgatg attataattt ggaaaaatta cgttaccaca aaatcattat tatgaccgat
     4861 gcggacgtgg atggtgcaca tattcgtacg cttttattaa ctttcttcta ccgtcaaatg
     4921 ccggagctga ttgaaaacgg ctatatttat attgcacagc caccgcttta taaagtgaaa
     4981 aaaggcaagc aagagcgtta tattcaagat aacgatgaaa tgtcgcaata tgaaattgat
     5041 attgcattag aaggtgcagg cttatatgtg agtgaagatg ctccagcgtt aagtggcgta
     5101 gcgttagaga acttaattgc ccaatataac ggcgtacaaa aattaattga acgtttaagc
     5161 cgtcgttacc caacattatt acttaacgaa ttaatttatt caaaaccgct ttcggtggaa
     5221 tttgcaaaaa atcaagcaaa tatgaccgct tggagcgagc aatttgtcgc tacattgatg
     5281 gcaaaagagg tgggcggcag tttctaccgt attaatacct tgttcaatga agaacgccaa
     5341 atttatgagc cggaaattat tgtgactaca cacggtatgg acagtaccta tcgtttagat
     5401 tacaacttta tcaacggtaa tgaatacgct cgcattgtgg cattaggcaa cgagctgaac
     5461 gatttactat ctgattcagc ctatgtggtg cgtggtgaac gtcgccaaga aatcagcagt
     5521 tttgctgaag ccttagattg gttggtgaaa gaatctcgta aaggcttaac tattcaacgt
     5581 tataaagggt taggtgagat gaatccggaa caattatggg aaaccacgat ggatccggtg
     5641 gctcgtaaaa tgctacaggt caatattacc gatgcgattg cggcagataa actcttcagt
     5701 acgctgatgg gcgatgaagt tgaaccacgc cgagatttca ttgaaactaa cgctttgtat
     5761 gctcagttag atatttagtt aaaaaatccg catttgacat gcggattttt tattctctta
     5821 aactagcatt ttaagcattc gctaaaatca ccgcccgctt cggagcaggg tagccttcaa
     5881 tggttttgct atgatcgttt ggatctaaaa aatccactaa actttcattt tccaaccaat
     5941 cggtttttcg ctgctcttct agtgtagtga ttgcctcatc aacacaacgc acatttttaa
     6001 agccgacttt ttccagccaa ttaatgagtg cagcaacaga aggaataaaa tagatatttt
     6061 tcattttcgc atagcgatca gccggcacta aaacatcatt aatatcgcca tcaatcacca
     6121 gcgtttctaa caccaattca ccgcctctga ctaattgggc tttgagttga gaaagatgat
     6181 caagcggtga tttgcgatga taaagcaccc ccattgaaaa cacggtatca aaagcagcaa
     6241 gcggttgcat ttgctcaata ccaagtggaa tcagattagc ccgtctatca ttccctaaga
     6301 gtttccgcac tgcctcaaat tggcacagga acagctcggt cgggtcaata ccaactacca
     6361 tttttgcccc ttcgcctacc attcgccaca tatgataacc gctaccgcag ccgacatcta
     6421 aaatggtgcg gtcttttaac ggtgcaagat gcggaagcac acgatcccat ttaaaatcgg
     6481 aacgccattc ggtatcaata tgaatcccgt gtagatgata agggccttta cgccacggca
     6541 ttagttgctt taaatggtgg gtaatttgct tgacttcccc ctcagataaa ggcttgcttg
     6601 aaatagactc aaccttgtct tttagattga tacaagcggt cgaatttggt aaaaaatctg
     6661 caacttttgc ccacttggca tagtcagcgt gggtggtctt ttcccattgt ttgagttgta
     6721 agggtaaggt ttctaaccaa gcagaaaggt cggttgtggc aatttgttga taaaaaggac
     6781 gaaagtcaat cattttgaaa tttatgtcta ggggagagac ctatgccctc ccgaaaggta
     6841 gtaaatagaa aggacaggca cagaacctac ccctacgaaa aatataaaat tatctcgcaa
     6901 tcggatttac tttcgctttt tgcactttgc gataagcgtt cagtaatttc tgatgtgcag
     6961 tcatattttc aagcgattcg tccgatgcag ttaaaccgtg gaacgggtta ccgccttgga
     7021 ttgcacccca agcagcttca accgcttgtt taccgtacat tttctcaaac accatacgat
     7081 attgttccga atcacgctct tcatctaaga acaactcaat cgattggatt aagcagcggt
     7141 aataattgtt acgttctgct gagaatacgc ttgcattcat attcgctgtc cagtttgccc
     7201 agtctgaggc ttgctctaag ttacctaaag ctaaatgcag catcgattta agctcaccga
     7261 tacgcaatgt ttgcattgca gagcctttct ctgccaccac gccaataaat tcacgcacac
     7321 gggttaaatc atcaatgctt tgctcatcta attcatcaag cagttcttgg taagtttcag
     7381 catcgtgatg gaaatgtggc aaatctaaca agatttcacg ccaatccata cccatattgt
     7441 tattggcata gaccaaatca tcgcttggat agatattcga catacccggt gcaagaatac
     7501 ggcaagcgta cacacctaaa tgctcataat ccataatata cacaggctga ttatgcttgt
     7561 tgaaaatcgc catcatatta ttaaattctt gctccgttgt gccggagaag tcccaatgca
     7621 cgaacggata atccgcatca cgtttgaaaa gatcccacga aattagaccg cttgaatcaa
     7681 taaagtgtgt ctcaaggttc gcatgatctg ctacatcttc attattaaat gaaggtgggg
     7741 caaacacatc taaatccttt aagctacggc cttgtaataa ctcggttacg gtacgttcaa
     7801 acgccacttg gaaattcggg tgtgcaccaa aagaggcaaa acaagtaccg ttatttgggt
     7861 ttaacaagat cacacaaata actggaaatt ctccgcctaa tgaagcgtca tagcataaaa
     7921 tcgggaagtc ttcttgctct agtttctcga tagaggcttt gattgtcgga taaccgtcaa
     7981 tcacactttg cggaatttcc ggcaagctga ttgcctcagt aataatacgg tttttgacaa
     8041 aacgctcgaa cacttcggat aagccctgca ctcgagcttc attttttgtg ttgcctgccg
     8101 acataccgtt tgaaacaaac aggttgccga taatgctttg cggaatgtaa accgtttcat
     8161 tgtcagattg gcgaacgtaa ggtagggtaa caattccacg atcataattt cctgattgta
     8221 aatcaaccaa taattccggc gtgatttcac cattcggatc aaaatattcc cataaaaact
     8281 cgtccatcat ctccattggt ggaatggctt catcatcaat cggaaaccat ttttcggttg
     8341 gataatgcac aaattcacta ttagcaatct cttgacctaa ataaaaatcc gcccagaaat
     8401 agttggtaga taaacgctca aaatactcac ctaatgcaga ggcaagagcg gcttttttgc
     8461 ttgctccctt accgttagaa aaacattgcg gacagtctgc atcacgaata tgcaccgacc
     8521 acacattcgg aacaggattt aaccaagagg cttcttcaat attaaaacct aaggttttta
     8581 atttatgctg aaaggtcgca atgctgtctt caagtgcagc atctttaccg gtaataaaag
     8641 tttgttctga catgataata cctttaactt gtcgtaaaaa tggcaacatt ctagcaaaaa
     8701 tccccataga taagtaaatt ttttgtgaaa tttctgctga aagaacagat ttcaaaactt
     8761 agaaaaaatg attacaagcg gtcaaatttt acttgaattt tgcaagtgaa atttgtgttt
     8821 gatttagatt aagaaaatat tatctaggat atatacagaa taaaaggtta gaggtagtat
     8881 tgcttgtgtt aggaaggaaa tattgaaggt ataaaaatgg aagaacatac tattcagtct
     8941 ttttatgccc attcggggaa gctttcagat cattcagatt ggcaaacttt actcagccac
     9001 gccaataatg ttggcttgct tgccgccgaa tttgctgcgc cgtttgcttc acaagaaatt
     9061 gctcgctgca cggggctatt gcacgattta ggcaaatata gtgaacgtta ccaaaagcgt
     9121 ttacatggcg gagaacgggt aaatcactct actgctggtg caaaaattgc agtggaacgt
     9181 tgggggcctt tgggcaaaat gatggcgttt tgtatcgcag gccaccacgc tgggttagca
     9241 aatggttctg gagaagggaa aaatcgttct acacttacac aacgtttgca agagcaattt
     9301 ggctatggtc ataaagatct accggtactt gacgctattt ggcagcaaga acttaatttg
     9361 cctgaaaaat tgcccttacc gccgttaaag ccttctaaaa acgaaccttt cttttcttac
     9421 gcctttttta tccgaatgct ctattcttgc cttgtcgatg ccgactttct tgatacagaa
     9481 gctttttatg ccaatctcga aaaaagaccg cttgaacgag gaaattaccc caatttaacc
     9541 gcactccatc agcaatttaa tcaatttatt gcacagttta gggagcgaat tgccactcaa
     9601 acgcctaata cgcaggaagc acaacgtaaa gcggaagtaa accgcttgcg gagtgaaatt
     9661 ttggattatg ccgtatctca agcaaaacag gaaacaggtc tgttttctct gactgtgcca
     9721 acgggcggtg gtaaaacctt tacttcaatg gcgtttgccc ttgagcatgc taaacagtat
     9781 ggtttatgcc gcattattta tgttattccg tttaccagca ttattgaaca aaatgctgcg
     9841 gaatttcgta aagcttttgg cgaattagga acagaggcgg tattggaaca tcacagcact
     9901 tttgatcacg atagcctacc tgataaatca agccgagata agctgaaatt ggcatcagaa
     9961 aattgggata ctcctgttgt ggttacaact gcagtgcaat tctttgaaag tttgtttgcc
    10021 gaccgttctt cacgctgtcg caaattgcat aatatcgtcg gtagcgtgat tattttggac
    10081 gaagcccaaa tgctaccgtt gaatttatta cgcccaatta tggctgcgat tgatgaatta
    10141 gctcgcaatt atcaatgttc ggtagtgctt tgcacagcga cacaacccgc tattcatcaa
    10201 gcgaacggtt tttataaagg atttgaaaat gtgcgagaaa ttgcccctaa tccaaccgca
    10261 ctttttgaaa aattgaaacg tactacggta aagcatatcg gtattcaaac agatgtggag
    10321 ttgcaaagca aactcgcaga taacccacaa atgctgataa tcgtgaataa ccgccgtcac
    10381 gcccgagcgt tatatgattc agcgaaacag ttagagggag cttatcacct caccacgcta
    10441 atgtgtgcta aacaccgctc acaaacgctg actgaaattc gtcttcgcct aaaacaaaac
    10501 ctaccttgcc gagtgattgc cacttcttta attgaagccg gtgttgatgt cgatttccca
    10561 ctggttatgc gagctgaagc aggactagat tcagtggctc aagcagcagg gcgttgcaac
    10621 cgagagggga aacgtttatc tgtagaaagc aatgtttggg ttttccaacc tgaagaacag
    10681 tggaaagcac caccagagct aggcttactt tctggctgta tgcgttcaac ggttcaggtt
    10741 cacgcagatg atctacttaa tccgaaagca atcaatcatt atttccaagc ggtttatgag
    10801 gctaaaggaa aagagctgga taagccaaat attttaaagg cgtgtcacga cgctgggcaa
    10861 agccttaatt tcccattcca aaaaatagct gaagatttcc gaatgatcac aagccattta
    10921 attccggtga ttatcccttt cgaccaagaa gccgaaaacc ttattgaccg tttacgctat
    10981 gcggatcaag taggcggtat tttaagaaaa ctccagcctt acactgtgca agtaccggaa
    11041 aaagcattag ccgaactcta taacgctggc agagttgaaa ccattaatga aaccaacttc
    11101 ggcaaacagt tttactgctt aattggaatg gatttgtatg atgaagtggc ggggttgagt
    11161 tgggagaatg tggattttct tgagtcggag aaattggttt tatagatgaa tgtttatttt
    11221 aacttcttta taagtttaat tttgcataga tatactttaa tataccaaat agcctatgac
    11281 gtctcgtagt aaacagctag tttgtaagaa tagatatttg gaagggaaac gtaaaatgag
    11341 agaaccatta ttagatgaag atgaattaaa aaaatggttg caaactattt ttactgacaa
    11401 tttggtcatt attgtcggtt cgggtttgtc ctgtgccgaa ggtttgccgg gaatgggagc
    11461 attagctgat agattaaaag aacgaatgcc tgaatgtctt gatgatatag ataaggtaac
    11521 atggaatacc atttctgatt gtctggattc tgaaggcttg gaaggtgcat tgttgaagca
    11581 tcaagcaaat gagacgatag aagccgctat tattaaaata acagccgagt atgtactctc
    11641 cgaagagcaa aaagcgataa acaaatgtat tgctgagaat cagaagctca aattttctta
    11701 tttgcttccc catatttcag cttcaaatcc taaaattgcc cgtgtaatta ctactaatta
    11761 tgatagattg attgagtttg cggcagagta tgaaaattgg ggaatagatt caatgatggt
    11821 cggtcgttat tggggaaaac ataatcctga tttgagtagg aaattgcaga taagagacat
    11881 tagagtaaaa ggtaaatgtc caaaattg
//
LOCUS       DANLXS010000069        11854 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.069, whole genome shotgun sequence.
ACCESSION   DANLXS010000069 DANLXS010000000
VERSION     DANLXS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.069"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            270..1634
                     /locus_tag="PXD71_001353"
     CDS             270..1634
                     /locus_tag="PXD71_001353"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDL6291471.1"
                     /translation="MTIKTPIQAVLASNQYFLDRQDAMESNVRSYPRKLPFAYQKAQG
                     VWVTDVEGNEYLDFLAGAGTLALGHNHPKLIQAVKDVLDSGLPLHSLDITTPLKDAFT
                     EELLSFFPKDKYILQFTGPSGADANEAAIKLAKTYTGRGNVIAFSGGFHGMTHGALSL
                     TGNLSAKNAVQGLMPGVQFMPYPHEYRCPFGIGGEAGAKAVEHYFENFIEDVESGVVK
                     PAAVILEAIQGEGGVVPAPISFLQKVREVTQKHGILMIVDEVQAGFCRSGKMFAFEHA
                     GIEPDIVVMSKAIGGSLPLAVLAIKKEFDAWQPAGHTGTFRGNQLAMATGYVSLKIMR
                     EENLAKNAQERGEYLTNALRELSKELPCIGNVRGKGLMMGIDIVDERQPKDATGAYPR
                     DCDLAVAIQKYCFKNKLLLERGGRGGNVVRVLCAVNITQAECEEFIKRFKQSVAEAVK
                     AVRG"
     gene            1724..3259
                     /gene="ddc"
                     /locus_tag="PXD71_001354"
     CDS             1724..3259
                     /gene="ddc"
                     /locus_tag="PXD71_001354"
                     /EC_number="4.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,4-diaminobutyrate decarboxylase"
                     /protein_id="HDL6291472.1"
                     /translation="MSNLEKHRQSLFCSDPKSIADYESAMNNAVKAVSDWLKEEKMYT
                     GGSIKELRAKINGFNPTQAGVGVQKSLEHLVEIFLKPSLKVHHPHSLAHLHCPTMVTS
                     QIAEVLINATNQSMDSWDQSPAGSIMEEHLIDWLRQKVGYGKGTSGVFTSGGTQSNLM
                     GVLLARDWAIANHWKKADGSEWSVQADGIPADAMQKVKVVCSENAHFSVQKNMAMMGM
                     GFQSVVTVPSNANAQMDVEALAKTLADLKAEGKIVACIVATAGTTDAGAIDDLKAIRK
                     LADEHQAWVHVDAAWGGALLLSKDFRHFLDGIELTDSITLDFHKHFFQSISCGAFLLK
                     DEANYRFIDYKADYLNSEYDEEHGVPNLVSKSLQTTRRFDALKLWFTVEALGEELYAS
                     MIDHGVYLSKEVEAYINATDGLEMLVPAQFASVLFRVVPQGYPAEFVDTLNQNVADEL
                     FARGEANIGVTKVGEHQSLKMTTLSPIATLENVRALLAQVLAEADRIKDSIVNGTYVP
                     PID"
     gene            3362..3724
                     /locus_tag="PXD71_001355"
     CDS             3362..3724
                     /locus_tag="PXD71_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HDL6291473.1"
                     /translation="MKKVLGLTLMAMALSACNSTASNGSSQANTGVAPANVISVKQVL
                     AAADDSYVTVEGTLLNKVGDETYLFKDATGQIRVEIDDEVWAGQHVGSEGNKVRLYGE
                     IDKGFNKTELDVKTLTVL"
     gene            complement(3814..4401)
                     /locus_tag="PXD71_001356"
     CDS             complement(3814..4401)
                     /locus_tag="PXD71_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6291474.1"
                     /translation="MNHLIIYAHPNPSSFNHAILEKTIAASVPNKVVVRDLYQLNFKP
                     ILDWAEFQGTLSKHYEADVAIEHQYWLEADVITLIYPLWWMGFPAILKGYLDRVLSYD
                     FAYRNGEIESIGLLTGKKMQQFVTLGNSNEKYERKGFLAAFEHTLGKGLFEFCGIRDV
                     KMHYFGNIGLKETDYTAILQQITASCSTILKEQAV"
     gene            4577..5125
                     /gene="napF"
                     /locus_tag="PXD71_001357"
     CDS             4577..5125
                     /gene="napF"
                     /locus_tag="PXD71_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDL6291475.1"
                     /translation="MTDYPNQSLPRRSFLRGQFLDSLKSQKVSQQGYEPIRPPWANLA
                     NFLEKCTACGNCIQACETQIIVKGAAGYPEIDFSRGECTFCEKCVHSCEAEVFRAVSE
                     QPWSHKIEIQAGCLLKLGTECRSCGDSCEMRVIRFRPSLGGIAEMVLNLESCNGCGAC
                     LSVCPTSAIKIQPLQTQANRDE"
     gene            5118..5384
                     /locus_tag="PXD71_001358"
     CDS             5118..5384
                     /locus_tag="PXD71_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDL6291476.1"
                     /translation="MSEKLSEQENWYVCSLVVQAKPEKLEEVKAEILKIPHTEIHGEK
                     TDEGKLVVTIESNVHLALSDRIDQVRDIKGVIVVSLISNYIDEQ"
     gene            5423..7921
                     /gene="napA"
                     /locus_tag="PXD71_001359"
     CDS             5423..7921
                     /gene="napA"
                     /locus_tag="PXD71_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDL6291477.1"
                     /translation="MELNRRDFMKANAAVAAAAAAGIMIPVKNVQADDTGIRWDKAPC
                     RYCGTGCSVLVGTKDGKVVATQGDPDAEVNRGLNCIKGYFLSKMMYGADRMQEPMLRM
                     KDGKFDKNGDFTPVSWDQAFSIMAEKIKTTIKNDGPNAVGMFSSGQTTIFEGYAKVKL
                     WKGGFRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMH
                     PILWSRISDRRLSDNKVKVVVMSTYEHRSFELADTPIIFKPHSDLAILNYIANYIIQN
                     DKVNWDFVNKHTKFKRGETNIGYGLRPEHKLQQNTNAKTAGKMYDSDFEEFKKIVAPY
                     TLEEAHRISGVPKDQLETLAKLYADPNQKLVSFWTMGFNQHTRGVWVNHMMYNVHLLT
                     GKIALEGCGPFSLTGQPSACGTAREVGTFIHRLPADMVVTNPKHREIVENSWKLPAGA
                     IPDVPGFHATAQSRALKDGKMKFLWQMCTNNMQGGPNINEEIFPGWRNPETFIVVSDP
                     YPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPANARSDTWQLVEFSKY
                     FKVEEVWPEELLAKAPELRGKTLYEILYRNGKVDSYQVPTNIPGYMNDEADHFGYYIQ
                     KGLFEEYAEFGRGHGHDLADFDTYHKVRGLRWPVVKDEKTGEYKETLWRYREGYDPYV
                     KAGEEVAFYGNADKKAVILGVPYEPPAEAPDEEYDLWFCTGRVLEHWHTGTMTRRVPE
                     LHKAFPTNLVWMHPNDAKKRGLRHGDKVKVISRRGEYVSNLDTRGRNKCPEGLIYSTF
                     FDAGQLINKVTLDATDPISFETDFKKCAVKVVKA"
     gene            8010..8915
                     /gene="napG"
                     /locus_tag="PXD71_001360"
     CDS             8010..8915
                     /gene="napG"
                     /locus_tag="PXD71_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDL6291478.1"
                     /translation="MKMDPNRRQFLKNATRTAAGVCGVGMILGLQQQQSFARQGVALR
                     PPGALPEQDFLSACTRCGQCVQACPYDMLHLASLISPVEAGTPYFIARDKPCEMCLDI
                     PCMNACPTGALSEELKDIDDARMGLAVLLDHETCLNWQGLRCDVCYRVCPLIDKAITL
                     EKVHNDRTSIHAKLIPTVHSDACTGCGKCEQACVLEEAAIKVLPMDLAKGLLGRHYRL
                     GWQEKQNAGKSLIEDVHPEGLRPAYDARMPEGLHEPVYQHMEVKPDVKVATPSRATQN
                     YVPNSTTVEAPEHFPDLDLNLKGVK"
     gene            8915..9793
                     /gene="napH"
                     /locus_tag="PXD71_001361"
     CDS             8915..9793
                     /gene="napH"
                     /locus_tag="PXD71_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDL6291479.1"
                     /translation="MAANSPKNAGLEARQKLGWWHAYRFLILRRLSQLSIILMFLSGP
                     LWNVWILKGNYSGSLLLDTVPMTDPLMTAEVLATGYRPEWTVLVGALIMVAFYGIVGS
                     KLFCSWVCPMNMVTDTAAWLRRKLGIRQTAKIPRNLRYVILAVILVGSAISGTLLWEW
                     INPVAALGRVFVFGLGATLWLVLVVFLFDLLVAEHGWCGHLCPIGATYALIGAKSLIK
                     VKVVDRNRCDNCMDCYNVCPEPQVLRVPLHGKPEESTVILSKDCITCGRCIDVCAENV
                     FAFGMRFQGSIDVKNI"
     gene            9839..10267
                     /locus_tag="PXD71_001362"
     CDS             9839..10267
                     /locus_tag="PXD71_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDL6291480.1"
                     /translation="MRKYLAFILAVMSGFAMAEAPKLSGSIDETTESVAPGFHNPQKD
                     VGTIPTTFPFQPPLVPHSIRGLQVSKNVNHCLGCHAIEPSKTTGATRLPPSHFTDRDG
                     KVYENESPRRYFCLQCHVQQTDVNPIIQNKFESVRESQAK"
     gene            10278..10886
                     /locus_tag="PXD71_001363"
     CDS             10278..10886
                     /locus_tag="PXD71_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL6291481.1"
                     /translation="MLRRFCNWLRSPSKMAIGAVILISALGGIFAWSGFNAGLAMTNT
                     EEFCSDCHMNDVVPEYRASAHYSNRSGVKAICSDCHVPHEFFPKWQRKIIAAKEVYAH
                     FTGKVDTKEKFEAHRGEMAEREWARMKANSSQECRNCHNFDDMDFTQQKNIAQQMHAL
                     AQEQGKTCIDCHKGIAHNLPHMEAIQKNFIPDDLLKKAEEKK"
     gene            complement(10948..11767)
                     /gene="cpdA"
                     /locus_tag="PXD71_001364"
                     /pseudo
     CDS             complement(10948..11767)
                     /gene="cpdA"
                     /locus_tag="PXD71_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
BASE COUNT         3451 a         2367 c         2753 g         3283 t
ORIGIN      
        1 attgctagat aaaagcttcc cttttcaata aaaaagagta tcatacgctc tcttaaacgt
       61 aaccctacat ttaagtctgt ttccaaccta tcccaatttc tttattcctt aggggcatat
      121 agggtaaaaa caaaaaagct gaaacgcttt ttttaataag tagcgagtca aatgccccat
      181 ggcatctatt cttttaggaa tagaaagctt aggctttccg caaggcaccc gacttatttt
      241 tttcacctct tgtagaaggt ttattttcaa tgacaattaa aactcctatt caagccgttc
      301 ttgcttcaaa ccaatatttt ttagatcgcc aagatgcaat ggaatcaaac gtgcgtagct
      361 acccacgtaa attacctttt gcttaccaaa aagcacaagg cgtttgggta actgatgttg
      421 aaggcaatga atacctcgat ttcctagcag gtgcaggaac gcttgcatta ggtcataacc
      481 accctaaatt aatccaagca gttaaagatg tattagacag tggtttacct ttacactcgt
      541 tagatattac gactccgtta aaagatgctt ttacggaaga attactctct ttctttccaa
      601 aagataaata tatcctccaa tttactggcc cgtctggtgc agatgctaac gaagcggcaa
      661 ttaaattagc caaaacctac actggtcgtg gcaacgtaat tgctttctcc ggtggtttcc
      721 acggaatgac ccatggtgcg ttatcgctca ccggtaattt aagtgcgaaa aatgcagtac
      781 aaggcttaat gccgggcgta cagtttatgc cttatccgca cgaataccgc tgtccgtttg
      841 gcattggtgg cgaagccggt gcaaaagcag ttgaacatta ttttgagaac tttattgaag
      901 atgttgaaag tggtgtggta aaaccggcag cggtgatcct tgaagccatt caaggtgaag
      961 gcggtgtggt tccggctcca atcagcttct tacaaaaagt gcgtgaagtc actcaaaaac
     1021 acggcatctt aatgattgtg gacgaagttc aagctggttt ctgccgttca gggaaaatgt
     1081 ttgcctttga acacgcaggc attgagccgg atatcgtagt aatgtcaaaa gcgattggtg
     1141 gtagcttacc gcttgcggta ttagcgatca aaaaagagtt tgacgcttgg caaccggcag
     1201 gtcataccgg tacattccgt ggtaaccaat tagcaatggc aaccggctat gtatcgctta
     1261 aaattatgcg tgaagagaat ttagcgaaaa acgcacaaga gcgtggcgaa tacttaacta
     1321 acgcattacg tgaattaagc aaagaattac cttgtattgg caatgtgcgt ggtaaaggct
     1381 taatgatggg aattgatatt gttgatgaac gtcagccaaa agatgcaaca ggtgcatacc
     1441 cgcgtgattg cgatttagcc gttgcaattc aaaaatactg ctttaaaaac aaattgttat
     1501 tagagcgtgg cggacgtggc ggtaatgtgg tgcgtgttct ttgtgccgtg aatattaccc
     1561 aagcagaatg tgaagagttt attaaacgct ttaaacaatc tgttgctgaa gcagtgaaag
     1621 cggtgcgtgg ttaatagtta gaatggtagg agcgaaacat ttttcgccct aatgtaaaaa
     1681 ggggcgaata tttattcgcc cctacaccaa taggatagaa ataatgtcaa acctagaaaa
     1741 acacagacaa tccctgtttt gtagcgatcc aaaatcgatt gcggattatg aatcggcaat
     1801 gaataacgct gtgaaagcgg tttcagactg gctgaaagaa gaaaaaatgt acaccggtgg
     1861 ctcaattaaa gagttgcgtg ccaaaatcaa cggttttaac ccaacccaag ctggtgtggg
     1921 cgtgcaaaaa tcactggagc atttggtgga aattttctta aagccaagcc tcaaagttca
     1981 ccaccctcat tctttggccc acttacattg cccgacaatg gtaaccagcc aaattgcaga
     2041 agtgttaatt aatgccacca accaatcaat ggactcttgg gatcaaagcc cggcaggttc
     2101 gattatggaa gagcatttaa ttgactggct acgtcaaaaa gtcggctatg gcaaaggaac
     2161 ttcaggagtc tttacctcag gtggtacgca atcaaacctg atgggcgtat tattggctcg
     2221 tgactgggcg atagcgaacc attggaaaaa ggcagacggt tcagagtggt cggtgcaagc
     2281 cgacggtatt cctgccgatg caatgcaaaa agtgaaagtg gtttgttccg aaaacgctca
     2341 cttctcggtg cagaaaaata tggcaatgat gggaatgggt ttccaatcgg tagtgactgt
     2401 accgtcaaat gccaatgcac aaatggacgt tgaagctctt gcgaaaacgt tggcggattt
     2461 aaaagccgaa ggcaaaattg tggcttgtat cgtcgcaacc gcagggacga ccgatgccgg
     2521 tgcgattgat gatttaaaag cgatccgtaa attagccgat gaacatcagg cttgggttca
     2581 cgttgatgct gcttggggtg gtgcattatt actgtcgaaa gatttccgcc acttcttaga
     2641 cggcattgaa ttaaccgatt ccattaccct tgatttccac aagcatttct tccaatcaat
     2701 ttcgtgtggg gcattcttgc taaaagatga agcgaattac cgctttattg attacaaggc
     2761 agactacctg aattcagaat atgacgaaga acacggcgtg ccgaatttag tgtcaaaatc
     2821 attgcaaact acccgccgtt ttgatgcgtt aaaattatgg tttaccgttg aagccttggg
     2881 tgaggagcta tacgcttcaa tgatcgacca cggggtttat ctttctaaag aagtggaagc
     2941 ctacattaat gccaccgatg gcttggaaat gttagtgccg gcacaatttg cctcagtctt
     3001 attccgtgtc gtaccacaag gttacccggc agaatttgtg gataccttaa accaaaatgt
     3061 ggcagatgag ttatttgctc gtggcgaagc taacattggt gtgaccaaag tcggtgagca
     3121 tcaatcactg aaaatgacta ctttaagccc gattgcaacg cttgaaaacg taagagcatt
     3181 attagctcag gtgttggcag aagcagatcg gattaaagat tcgattgtaa atggaactta
     3241 tgttccgcca attgattaaa ctgcgttaaa aataaaaagg tgttttcgac atttatttgt
     3301 gttttaatgt ttgcattcgg tagtctttat cgaatgcaaa tttttttaac aaggagtttt
     3361 tatgaaaaaa gtcctgggtt taacattgat ggcaatggcg ttatcggcgt gtaattcaac
     3421 tgcttccaac ggttcttcac aagcaaatac aggtgttgca ccggcgaatg tgattagcgt
     3481 gaaacaggtg ttagcggcag cagatgacag ctacgtgacg gtagaaggca cactcttaaa
     3541 caaagtcggt gacgaaactt acttatttaa agatgcgaca gggcaaatcc gtgttgagat
     3601 tgacgatgaa gtatgggcag gtcagcacgt tggtagcgaa ggcaataaag tccgtttata
     3661 cggagagatt gacaaaggtt tcaataaaac cgagttagat gttaaaacac tcactgttct
     3721 gtagaagatc aaaccttaat ttggttcccc gccttgtttg gcggggtttt tacgttaatt
     3781 aatcaaacca tccttttgca aaaaaacgca aaattatacc gcttgttcct tcaatattgt
     3841 gctacaagat gctgtgattt gctgcaaaat agcggtgtaa tcagtctctt ttaaaccaat
     3901 attaccgaaa tagtgcattt ttacatcacg aatcccacag aactcaaaca gcccttttcc
     3961 cagcgtatgt tcaaatgctg ctaaaaagcc ttttcgttca tatttttcat tggaattacc
     4021 gagcgttaca aattgttgca tttttttgcc ggtcagcaag ccgatacttt caatttcccc
     4081 gttacgatag gcaaaatcat aacttaacac cctgtctaaa tagcctttta aaattgccgg
     4141 aaagcccatc caccaaagcg ggtaaattaa cgtaatcaca tcagcttcaa gccaatattg
     4201 atgctcaatt gccacatctg cttcatagtg tttactgagt gtgccttgaa attccgccca
     4261 gtctaatatc ggcttgaagt tgagttggta gagatctcgt acaaccacct tatttggaac
     4321 ggaagctgct atcgtttttt ccaaaatcgc atggttaaag ctggagggat tcgggtgtgc
     4381 gtaaataatc aggtgattca tagcgttttc ctttactgtg tcgaggcagc tactagaaga
     4441 ttttagcgaa actttctttg atgaaaatca atattttggc gaatataccg gcaaataccc
     4501 cgtttgagga atgaacggtt ttaagtgaac attagataat ttagcctatt atagtgatga
     4561 gtaaaaaggt gttttcttga ctgattatcc taatcaatca cttcctcgcc gtagcttttt
     4621 gcgagggcaa tttttagact cgttaaaaag ccaaaaagtc agtcagcaag ggtatgagcc
     4681 gattcgtccg ccttgggcta atttagcaaa ttttttagaa aaatgcaccg cttgcggtaa
     4741 ctgcattcag gcttgcgaaa cccagattat tgtaaaaggt gctgcgggct accctgaaat
     4801 tgattttagc cgtggtgaat gcactttttg tgaaaaatgt gtgcattctt gcgaggcaga
     4861 ggtattccga gcagtttcag aacagccttg gagccataaa attgagattc aagccggctg
     4921 tttattaaag ctcggaacgg aatgccgaag ctgtggcgat agctgtgaga tgagagtgat
     4981 tcgtttccgc ccgagtttag gtggcattgc cgaaatggta ttaaatttgg aaagctgtaa
     5041 tggctgtggg gcttgtttga gcgtttgtcc gacaagtgcg ataaaaattc agccattaca
     5101 aacacaggca aacagagatg agtgaaaaat tatccgaaca ggaaaactgg tatgtgtgca
     5161 gtctcgtggt gcaagcaaaa ccggaaaaat tggaagaagt aaaagccgag attctgaaga
     5221 ttcctcatac cgaaattcac ggtgagaaaa cagatgaagg caaattggtc gtgacgattg
     5281 aaagcaatgt gcatttagcc ctttccgatc gaatcgatca agtaagagat attaaaggcg
     5341 tgattgtggt gtctttaatt tctaattaca ttgatgaaca gtgattaatg attatttttg
     5401 aagccaaacg tgggacatag ttatggaact caatcgtaga gattttatga aagcgaacgc
     5461 cgctgtggca gctgctgcag ccgcaggaat tatgattcct gtgaaaaacg ttcaggcaga
     5521 tgacaccggt attcgttggg ataaagcccc ttgtcgttac tgtggtacag gttgtagcgt
     5581 gctagtcggt actaaagacg gtaaagtagt ggcaacccaa ggtgacccgg atgcagaagt
     5641 aaaccgtggt ttaaactgta ttaaaggtta tttcttatcc aaaatgatgt acggtgcaga
     5701 ccgtatgcaa gagccaatgt tgcgtatgaa agacggtaag ttcgacaaga acggcgattt
     5761 cacgccggtt tcttgggatc aagcctttag cattatggcg gagaagatta aaaccactat
     5821 taaaaatgac ggcccgaatg ctgtaggtat gttctcatca ggtcaaacaa ccatttttga
     5881 aggttatgcg aaagtgaaac tttggaaagg tggtttccgt tcaaatacga ttgacccgaa
     5941 tgcccgccac tgtatggcgt ccgctgcggt tgcctttatg cgtacctttg gtatggacga
     6001 gccgatgggc tgctataacg acatcgaaaa aaccgatgcg ttcgtgcttt ggggttcaaa
     6061 tatggcagaa atgcacccga ttttatggtc tcgtatttct gatcgccgtt tatcagacaa
     6121 taaagtgaaa gtggtggtaa tgtccactta cgaacaccgt tcgtttgaat tagccgatac
     6181 accgattatc ttcaaaccac actcagattt agcaatttta aactacattg cgaactacat
     6241 tatccaaaac gataaagtaa attgggattt tgttaacaaa cacaccaaat ttaaacgggg
     6301 tgaaaccaat atcggttacg gtttacgtcc tgaacacaaa ttacaacaaa ataccaatgc
     6361 gaaaactgcc ggtaaaatgt atgacagcga ttttgaagag tttaagaaaa tcgttgcccc
     6421 ttatacatta gaagaagctc accgtatttc aggcgtgccg aaagatcagc ttgaaacatt
     6481 agcgaaatta tatgcagatc ctaaccaaaa attagtgtcg ttctggacga tgggctttaa
     6541 ccaacatacc cgtggtgtgt gggtaaacca tatgatgtac aacgtccatt tattaaccgg
     6601 taagatcgca ttagaaggtt gtgggccgtt ctcactcacg ggtcagccgt ccgcttgtgg
     6661 tactgcccgt gaagtgggta catttattca ccgcttaccg gcagatatgg tggtcacaaa
     6721 tccgaaacac cgtgagattg tagagaattc ttggaaatta ccggctggag caattcctga
     6781 tgtaccgggc tttcacgcta cagctcaaag ccgtgcctta aaagacggca aaatgaaatt
     6841 cttatggcag atgtgtacca acaatatgca gggcggcccg aatatcaatg aagagatctt
     6901 cccgggctgg cgtaatcctg aaacctttat tgtggtttca gatccgtacc catctgtttc
     6961 agcagtggct gctgacttaa ttcttccaac ctgtatgtgg gtggaaaaag aaggggctta
     7021 cggtaacgca gaacgccgta cccaattctg gcgtcagcaa gttaaagggc cggcaaatgc
     7081 ccgttcagat acttggcagt tagtagagtt ctctaaatac ttcaaagtag aagaagtatg
     7141 gccggaagag ttattagcta aagcaccgga attgcgtggt aaaactctat acgaaatcct
     7201 ttaccgcaac ggcaaagtag atagctacca agtgccaact aacattccgg gctatatgaa
     7261 tgacgaagca gatcacttcg gttactacat tcaaaaaggc ttatttgaag agtatgcgga
     7321 atttggccgt ggtcacggtc acgatttagc cgactttgac acctaccaca aagtacgtgg
     7381 cttacgttgg ccggtggtaa aagacgagaa aaccggcgaa tacaaagaaa ctttatggcg
     7441 ttaccgtgaa ggctatgacc catacgttaa agccggtgaa gaagtggcat tctatggcaa
     7501 tgcggacaaa aaagcagtga ttttaggtgt gccatacgaa ccacctgcgg aagcaccgga
     7561 cgaagaatat gacttatggt tctgtaccgg tcgtgtacta gaacattggc acaccggtac
     7621 gatgacccgc cgtgtgccgg agttacataa agcattccca accaacttgg tttggatgca
     7681 cccgaatgat gcgaaaaaac gaggcttacg tcacggtgat aaagtgaaag tgatttcacg
     7741 ccgtggtgaa tatgtatcga acttagatac ccgtggacgt aataaatgtc cggaaggttt
     7801 gatttactcg actttcttcg atgccggtca gttaatcaac aaagtgacac ttgatgcgac
     7861 tgatccaatt tcatttgaaa ccgacttcaa aaaatgtgcg gttaaagtgg tgaaagctta
     7921 attgtagggg caaattattt gcctcagaca agcggtcaaa tttgcaaatt tttttacaaa
     7981 aacgaccgct tacctaagag gaacccacaa tgaaaatgga tccaaatcgt cgccagtttt
     8041 taaaaaatgc aacccgtacc gcagccggtg tttgcggtgt aggaatgatt ttagggctac
     8101 agcaacaaca aagttttgcc cgtcaagggg tagcactacg cccaccgggt gcattgccgg
     8161 agcaagattt cctgagtgct tgcacccgtt gcggacaatg tgtgcaggct tgtccttacg
     8221 atatgttgca tttggcatcg cttatttcac cagtggaagc cggcacgcca tactttattg
     8281 cacgggataa accttgcgaa atgtgcttgg atattccctg tatgaatgct tgcccaaccg
     8341 gggcattaag cgaagagcta aaagacattg acgatgcacg aatggggctt gcggtgttgc
     8401 tcgaccacga aacttgtctt aactggcaag gcttgcgttg tgacgtgtgt taccgtgttt
     8461 gtccgttaat cgacaaagcg attactcttg agaaagtgca taacgaccgt acctcgattc
     8521 acgccaaact gattccgacc gtgcattctg atgcttgcac cggttgcggt aaatgtgaac
     8581 aggcttgcgt attggaagag gcagcgatca aagtgttacc gatggattta gccaaaggct
     8641 tgctcggtcg tcactatcgt ttaggctggc aagagaaaca aaatgccggt aaatcgctga
     8701 ttgaagatgt tcacccagag ggtttacgcc cagcttatga tgccagaatg ccggaaggct
     8761 tacacgagcc ggtgtatcag catatggaag taaaacctga tgtgaaagtg gctacgccaa
     8821 gtcgggcgac acaaaactat gtgccgaact ctacgacggt ggaagctccg gagcatttcc
     8881 ctgatttaga cttgaatctc aagggggtga aataatggca gccaattctc caaaaaatgc
     8941 gggcttagag gctcgtcaga aactaggttg gtggcacgct taccgctttt tgattttaag
     9001 acgcttaagc caactcagca ttattttgat gtttttaagc ggcccacttt ggaatgtctg
     9061 gattttaaaa ggaaactaca gcggtagctt attactagat accgtgccga tgacagaccc
     9121 attaatgacc gctgaagtgc tggcaaccgg ctatcgccct gaatggacgg tattggttgg
     9181 ggcgttaatt atggtggcgt tttacggcat tgtcggcagc aagctgtttt gtagttgggt
     9241 ctgcccgatg aatatggtaa cagatactgc tgcttggtta agacgcaaac tcggtattcg
     9301 ccaaactgct aaaattccac gcaatttacg ctatgtgatt ttggcggtga ttttagtcgg
     9361 tagtgcgatt tccggaacac tattgtggga gtggatcaac ccggttgcgg ctctcggtcg
     9421 ggtgtttgta ttcggtttag gggcaacgct ttggctggtt ttagtggtgt tcttgtttga
     9481 cttattggtg gcagaacatg gctggtgtgg tcatctttgc ccgattggtg caacgtatgc
     9541 cttaattggg gcgaaaagcc tgattaaagt caaagtggta gatcgtaacc gttgcgataa
     9601 ttgtatggat tgttacaacg tttgtccgga acctcaagtg ttgcgtgtgc cgctacacgg
     9661 taaaccggag gaaagcacgg ttatcttatc taaagattgc attacctgcg gacgttgtat
     9721 tgacgtttgt gccgaaaatg tatttgcctt cggaatgcgt tttcaaggca gtattgatgt
     9781 gaaaaatatt taatttttat ctttttgaaa acaagaattt taatggagtg aacctcaaat
     9841 gagaaaatat ctcgccttca ttttagccgt gatgtccggc tttgcaatgg cggaagcccc
     9901 taagttatcc ggatcaattg atgagacaac cgaaagtgtt gcaccgggat ttcataaccc
     9961 acaaaaagat gtgggcacga ttccaaccac tttccctttc caaccaccgc ttgttccaca
    10021 tagtattcgt ggcttacaag tgagcaaaaa cgtaaaccat tgtttaggtt gccacgctat
    10081 tgaaccatct aaaaccacag gtgcgacccg tttaccgcca agccatttta ccgaccgtga
    10141 tggcaaggtg tacgaaaacg aatctcctcg tcgttatttc tgtttgcaat gccacgtgca
    10201 acaaactgat gtaaacccga ttattcaaaa taaatttgaa tctgtacgtg aatctcaagc
    10261 taaataagga gtaagtaatg ttgagaagat tttgtaactg gttacgtagc ccaagcaaaa
    10321 tggcaattgg tgctgtaatc cttatttctg cattaggtgg tattttcgct tggagtggtt
    10381 ttaatgcagg gttagcaatg acaaatactg aagagttttg ttcagattgc catatgaacg
    10441 atgttgtgcc ggaataccgt gcctctgctc actattcaaa ccgcagcggt gtgaaagcga
    10501 tttgttcaga ttgccacgta ccgcacgaat tctttccaaa atggcaacgt aaaatcatag
    10561 ccgcaaaaga agtttatgca cactttaccg gtaaagtaga taccaaagag aaatttgaag
    10621 ctcatcgtgg cgaaatggca gaacgtgaat gggcacgtat gaaagcgaac agctctcaag
    10681 agtgccgtaa ctgtcataat tttgatgata tggacttcac tcaacaaaaa aacattgcgc
    10741 agcaaatgca cgcattggca caagagcaag gtaaaacctg tattgactgc cacaaaggga
    10801 ttgcacacaa tctcccacat atggaagcga tccagaaaaa ctttatccct gatgatttgc
    10861 tgaaaaaagc agaagagaag aaataactta acgtgtttca atagaaaacc ctcaatttcc
    10921 attgagggtt tctttttagt agccaagtta gtaaccaatg gcattgacat ttggtaaaaa
    10981 atcgttggaa tttaaccgct tgacgaccgt ttctatcgtg ccgttttcgt gcaattcaat
    11041 ttcacgccag ccttgtggtt gtaagtctaa ggtaaactgg tcgcaattcg gcttaaattg
    11101 aatgcaagta gatggggtgg cataaatcgg aataccatgc caaatatcat caacctgctg
    11161 atgaatatga ccatgcacaa tcgcttttac attcttaaat tttgcgaaaa tctcggataa
    11221 ttcattgctg ttttttaagc tatgctgatc cagccacgct gagtttgtcg gcaaaatatt
    11281 gtggtgcaga gcaacaccgt tgcggatatt gtgccaattt ttcttctaac catttaagct
    11341 gtgagtaaga gagttcgcca taaggcatac ctaccacctg ggtatttagc atcagaagct
    11401 gccattgctc accgagtaaa atttgtttat ggggaaggat atgtttatat tgcgccaaaa
    11461 tttctgacat actcggctga acatcgtgat tcccctctag ccacactatc ggcgagttta
    11521 gcgaattagt tatctgtgca aaatagtgat aaccagcaat attgttatct tgaatcaaat
    11581 cacctgtggc aagaactaga tcaaaatatt gcttcgcttg tttgtttatt tgctcaataa
    11641 ctgccgaaaa actttttact gttttgacat taagtagagt ttcctccgga ttggagaaaa
    11701 ggtgagggtc agttatttgt aataacttaa ttacccctgt tacgggtaac ttaatatatt
    11761 cgtgcattcg ttttgcatcc attcaaattc tagtaatttt taactggtga gattctatca
    11821 gtaagctaaa agtaaaaatg tggactatgc cgca
//
LOCUS       DANLXS010000070        11501 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.070, whole genome shotgun sequence.
ACCESSION   DANLXS010000070 DANLXS010000000
VERSION     DANLXS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11501
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.070"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            170..1360
                     /gene="malE"
                     /locus_tag="PXD71_001365"
     CDS             170..1360
                     /gene="malE"
                     /locus_tag="PXD71_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDL6291482.1"
                     /translation="MKNKCVKFTLATLATLVLSGTVMAKMTEGKLVIWINGDKGYNGL
                     AEVGKKFEKDTGVQVLVEHPDRLEEKFAQVASTGDGPDIMFWAHDRFGGYAQSGLLAE
                     VNVSKEFKDKFVDFAWDAETYNGKIIGYPVAIEAISLIYNKDLVKEAPKSWEEIIELD
                     KKLKKEGKNAIMWNLSEPYFTWPIAASNGAYAFKFANGKYDPKDIGVNNEGAVKALQF
                     VVDMVKNKHISADMDYAVAEASFNKGQSALTINGPWSWGNIDKSGIKYGVTLLPTLNG
                     QASKPFVGVLSAGVNSSSPNKDLAKEFLENYLLTDDGLDAVNKDKPLGAVALKSYQEK
                     LAADPRIAATMANAKNGEIMPNIPQMSSFWYAEKSAINNAVTGRQSVKEALDDAHSRI
                     QKQQ"
     gene            1479..3017
                     /gene="malF"
                     /locus_tag="PXD71_001366"
     CDS             1479..3017
                     /gene="malF"
                     /locus_tag="PXD71_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDL6291483.1"
                     /translation="MLTQTQSKSTHWLKYLLAGLVLLLDFYLVVLMYSQGEYLFAILT
                     LIILTSGVYIFTNKNTYAWRYVYPGITGMAIFILFPLVATIAIAFTNYSGSNQLSFER
                     AVSVLTEQRYFAGDKYQFTLYPQADNKYQIALTNPTTEQTFVSEPISLATGTNVVVTE
                     QNRPTGEVAPLKVITQNRAALQSMKVVLPNNNELTMSSLRQFSEQKPRYQFDEENKIL
                     LNNETGKRYQANDETGFFQSIDENGHWQSETLEPGYTVTSGFNNFIKIFTDEGIQKPF
                     VQIFIWTVIFSLLTVVFTVILGMVLACLVQWEALKGKAVYRVLLILPYAVPSFISILI
                     FKGLFNQSFGEINMILNQLFGISPEWFNDPLLAKVMILIVNTWLGYPYMMILCMGLLK
                     AIPADLYEASAMDGASTWQNFSKITFPLLLKPLTPLMIASFAFNFNNFVLIQLLTNGR
                     PDMIGTTTPAGYTDLLVSYTYRIAFEGSGTQDFGLAAAIATIIFLLVGGLALLNIKAT
                     KMEL"
     gene            3070..3960
                     /gene="malG"
                     /locus_tag="PXD71_001367"
     CDS             3070..3960
                     /gene="malG"
                     /locus_tag="PXD71_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDL6291484.1"
                     /translation="MAIVQSKSVRYRVWATHFILICFLALIIFPLLMVIGISLRPGNL
                     AIGDIIPSQISWEHWQAALGFDVTHADGTVTPPPFPVLRWLWNSIKVATITSIGIVTL
                     STTCAYAFARMKFKGKKTILQGMLIFQMFPAVLSLVALYALFDRLGQYVPFLGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDGSLEEAASLDGATPWQAFRLILLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLYPQNYLWGDFAAAAVLSAIPITL
                     VFLLAQRWLIGGLTAGGVKG"
     gene            4035..6092
                     /locus_tag="PXD71_001368"
     CDS             4035..6092
                     /locus_tag="PXD71_001368"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDL6291485.1"
                     /translation="MKNALPYLCLFYTSSLFAQSWQHSHFSDFNDDLEKHFFQSQVQL
                     EKGSYPLRFKFGEQCYQPQQAIKLNQSVALVPCINEAPQLRLFRQGNYLAQIDLRSGT
                     PTPTISVEQQLQHNEAMFQSCPNWDKKPIEIDVSSTFAEGESVSDFYSGNTAIVKNGK
                     VTLMPNENASGLILLEKSNTENTATFDWKNATVYFVLTDRFYNGDPTNDHSYHRQKDG
                     MQEIGTFHGGDLKGLTEKLDYLQQLGVNVLWISSPLEQMHGWVGGGDKGDFPHYGYHG
                     YYHLDWTKLDANMGTEDDLSRFVQQAHQRGIRVLFDVVMNHTGYATLADMQEFNFGGF
                     YLTPDEISRTLGEKWTHWKPKSGQNWHSFNDFIRFNDNQAWQNWWGKEWVRADIADYD
                     SPKFDDLKMSLAALPDLKTESEQAVKLPEFFANKNTNAKTLPNAKVRDYLIGWLSDWV
                     RKYGIDGFRVDTAKHVEKSTWLVLKQSAQQALNEWQKANPNAGFNDRFWMTGEAWGHG
                     VFKSDYYQNGFDAMINFDFQDQAKQGLNCFAHIEPIYAEMNRKLSDFNVLSYLSSHDT
                     RLFFHSDSEQNIEKHKTAANLLLLSPGAVQIYYGDESGRTFGATGSDLIQGTRSDMNW
                     QELQQDPQKQALHQHWQKLTQFRQRHPAIGAGVHQLIKNDSYFAFKRVLNEDKVMVVW
                     AGN"
     gene            complement(6206..8932)
                     /gene="malT"
                     /locus_tag="PXD71_001369"
     CDS             complement(6206..8932)
                     /gene="malT"
                     /locus_tag="PXD71_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDL6291486.1"
                     /translation="MLIPSKLVCSFRLQNSIPRKRLIQELDKSCSYPVVLINAPAGYG
                     KTTLVSQWIENKKNIGWYALDEGDNNSDRFATYFSAALHSAINEEIDVLLEENRKANL
                     LALFNQLLIKAAAFSEHFYLAIDDYHIIENDEIHEALKYWIKHQPSNMTLILISRSIP
                     PLSIASLRVQEQLLEIDINQLMFDHQESTAFFQARLGSELNQQDVIALCNEVEGWPTA
                     LQLISLFAKNKSHTLQVPLQDIAKRLAKSNHFHINEYLADEVLNKVDEETRLFILRCS
                     VLHSMNESLVQAVTGEQNSRTKLESLEKQGLFLQQMANSKWQSSDDSWWKFHPLFASF
                     LNLCCQNELYAELPQLHQKAANAWLQLGYVTEALHHAMQLSDTGLLLNILSKHAWTVF
                     HQGELKLLEESLNCLDYAHLTEHTNLVLLKAWLVQSQHRHIEVGGILADFSRALTENK
                     IELSKTANAEFNVLRAQVAINSGDENTALQLASEALNDLSENAYYAHIVATSIIGEAH
                     HCHGNLAEALAMLQKAERMARQHHTYHNILWSQLQQSEILLAQGFSQAAYEMLDKASE
                     FVKENHLQKVPMYEFLLRSKGKILWEWYNLDKAESMAVAGMNALSKFEDKLQCLTLLT
                     KISLARGNLDNTARLLGEVEQLEHSHSYHHDWIANADQVRIFYWQMTNDVSAVRNWLI
                     QNPSPVSDKNHFTQVQWRNIARSRILLEEYHEAKAILDNLIETAEKFSLISDLNRALI
                     VRNRLYFLQGEKELAQQDLIQALKLTRQTNFISAFVIEGDVMAQQIRHLLQLNVLDEL
                     VLHKAQFILRNINQFYRQKFAHFDENFVNQLLKNPKVPELLKISPLTQREWQVLGLIY
                     SGYSNEQISDELQVAATTIKTHIRNLYQKIGVTNRNEAISYTKELLMLMGYH"
     gene            9096..11465
                     /gene="malP"
                     /locus_tag="PXD71_001370"
     CDS             9096..11465
                     /gene="malP"
                     /locus_tag="PXD71_001370"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDL6291487.1"
                     /translation="MTFNSIVEKYCRYFDVTDPKHFTLQQWYQVVAEGSLELIYSQPA
                     TKPTESRHVNYLSMEFLIGRLTGNNLMNLGYYEQIRDYLKQYQVELVDVLEQERDPAL
                     GNGGLGRLAACFLDSMATVGQNATGYGLHYQYGLFKQSFKEGMQKEAADTWARDSYPW
                     HRFNPSKTRHIGFGGKIKHIQADQYEWQPKLTIQGKAFDLPIVGYKNNIIQPLRLWQA
                     DSDQSFDFDAFNEGKFLSADKTIVNAAALTQVLYPNDNHQAGQKLRLMQQYFHCACSV
                     ADILDRHISEGYSLEDLAKRQVIQLNDTHPTLAIPELMRVLLDEHQFTWEQAWDICSN
                     TFAYTNHTLLPEALEQWDQRLFKQLLPRHYQIVEKINDIFHDKVQAEFGDDAKIWEKL
                     AVLFDYRVRMANLCVVTCFRVNGVAEIHSDLLVTDLFPEYHKLFPTKFCNVTNGITPR
                     RWIRQANPQLSDLLDRTLKQDWTHNLELLKGVEQYADDTGFREEYQRIKRNNKVVLAN
                     EIHKTLGLNVNPNAIFDVQIKRFHEYKRQHLNLLNIIATYQSLKANPNQDYTPRLFLF
                     SGKAAPGYYLAKNIIHAINNVADIINNDKQVNDRLQVAFLPDYRVSLAEKIIPAADVS
                     EQISMAGKEASGTGNMKLALNGALTLGTLDGANVEIADMVGEENVFIFGHTVESVREL
                     LAKGYQPKAYYNQDPILKNAVDFLATGKASNGDKEAFKLMLDSLLERDPFLVFADFDS
                     YRQAQEKIGVAYLDQETWLRSAILNTARLGAFSSDRSIKDYQQRIWLNR"
BASE COUNT         3484 a         2317 c         2273 g         3427 t
ORIGIN      
        1 tgaagcagat cacaaaatga taaaaaatgt tacttagatc atacttttag cttggggggc
       61 gtagagcaaa gggtgatctc aaatagattg tatagcccat aatttgccat aatttaataa
      121 atgggttgtt tcatatccgt ttcttttatt taacttcaag gagtttttta tgaaaaacaa
      181 atgtgtcaaa ttcaccttag ctactcttgc taccttagtt ctttcaggaa cagtaatggc
      241 aaaaatgacg gaaggtaaac tcgttatctg gattaacggt gataaagggt acaatgggct
      301 tgccgaagtc ggtaagaaat ttgaaaaaga taccggtgtt caagttttag ttgaacaccc
      361 ggatagactt gaagaaaaat ttgcacaggt tgcctcaact ggtgacggtc cggatattat
      421 gttctgggct cacgaccgct ttggtggtta tgcacaatcc ggtttattgg ctgaagtgaa
      481 tgtaagcaaa gaatttaaag ataaatttgt cgattttgct tgggatgctg aaacttacaa
      541 cggcaaaatt atcggttatc cggttgcgat tgaagcgatt tcattaattt ataataaaga
      601 tttagtgaaa gaagcaccaa aatcttggga agaaattatt gagttagata aaaaactcaa
      661 aaaagagggc aaaaatgcca ttatgtggaa cttatccgag ccctatttca cgtggccgat
      721 tgcggcttca aacggagctt atgcattcaa atttgctaac ggcaaatacg atccaaaaga
      781 tattggtgtg aacaatgaag gggcagtaaa agcactacaa tttgtagtcg atatggtgaa
      841 aaataaacat attagtgcgg atatggacta tgccgttgcc gaagcctcct ttaataaagg
      901 acaaagtgcc ttaaccatca atggtccttg gtcttggggc aatattgata aaagcggtat
      961 taaatatggt gtaactttac tcccaacatt aaacggtcaa gcttctaaac cattcgtagg
     1021 ggtgttaagt gccggtgtaa acagctcaag cccaaataaa gatttagcca aagaattctt
     1081 agagaattat ttactcaccg acgacggttt agatgcagtc aataaagata aacctttagg
     1141 tgcggtggcg ttaaaatctt accaagagaa attagcggct gatcctcgta ttgctgcaac
     1201 gatggcaaat gcgaaaaacg gtgaaattat gccaaatatt ccgcaaatgt cttctttctg
     1261 gtatgccgaa aaatctgcta tcaataatgc agtgaccggt cgtcaatcgg tgaaagaagc
     1321 attagatgat gcacattcac gcattcaaaa acaacaataa ttcttcatca ttctgagtgg
     1381 gttattatga cccactctat ttttagattc accctaaata caataaaagt acagtaaaag
     1441 taccgtaaca aaatgcagta aaaattcgag gtgctgttat gctaactcaa actcaatcca
     1501 aatccactca ttggctcaaa tatctcttag ccggattagt cttgctcctt gacttctatc
     1561 ttgttgtctt aatgtactca caaggtgaat atctttttgc tattttaacg ctgattattc
     1621 taacttccgg tgtctatatt tttaccaata aaaataccta tgcttggcgg tatgtttacc
     1681 ctggcattac cggaatggca atttttattt tgtttccgct ggttgcgact attgcaattg
     1741 cgtttaccaa ctatagtggt tctaatcaat tatcctttga acgagcggta tccgtcttaa
     1801 ctgaacaacg ctattttgcc ggagataaat atcaattcac cctttatccg caagcagata
     1861 ataaatatca aattgcatta accaatccga cgacagagca aacttttgtg tcagagccga
     1921 tttcattggc taccggaacg aatgtggttg taaccgagca aaacagacca actggcgaag
     1981 tcgctcccct taaagtgatt acgcaaaatc gggccgcact tcaatcaatg aaagtggttt
     2041 taccgaataa taatgaactg acaatgagct cattacgtca attctccgag caaaaaccac
     2101 gctatcaatt tgatgaagaa aataaaatat tactcaataa tgagactggc aaacgttatc
     2161 aagcaaatga cgaaaccggt ttcttccaat cgattgatga aaacggtcat tggcaaagcg
     2221 aaacacttga accaggttat actgtcacat caggctttaa taacttcatt aaaattttta
     2281 ccgatgaagg tattcaaaaa ccttttgtcc aaatatttat ttggacggtg atcttctctt
     2341 tattaaccgt tgtctttacc gttattctcg gtatggttct tgcctgtttg gtacaatggg
     2401 aggcattaaa aggtaaagca gtctatcgtg tcttattgat tttaccttat gccgtaccat
     2461 catttatttc gattctgatt tttaaaggac tatttaacca aagttttggt gagatcaata
     2521 tgattttaaa ccaactattc ggcatcagcc ctgaatggtt taacgatccg ctccttgcca
     2581 aagtgatgat cttaattgtc aatacgtggt taggttatcc ttatatgatg attttatgta
     2641 tggggttact caaagctatt ccggctgatt tatatgaagc ctccgcaatg gacggtgctt
     2701 ccacttggca aaacttcagt aaaatcactt tcccattact gttaaaacca cttactccgt
     2761 taatgattgc ttcgtttgca tttaacttta ataactttgt attaattcaa ctattaacaa
     2821 acggtcgccc ggatatgatt ggaacaacca ctccagccgg ttataccgat ttattagtca
     2881 gctacaccta tcgtattgcc tttgaaggaa gtggtacgca agatttcggc ttagcagccg
     2941 ccatcgcaac cattattttc ttattagtcg gtggcttggc actcttaaat atcaaagcaa
     3001 ccaaaatgga attataaaag cgggagggat ttaacaatcc cgaccccaaa ctaactttag
     3061 gagttatata tggcaattgt tcaatctaaa tctgtgcgtt atcgggtatg ggcaacccat
     3121 ttcatcttaa tctgtttcct tgccttaatt attttccctt tattaatggt gattggcatt
     3181 tcgttacgac cgggaaatct ggctatcggt gatattattc caagccaaat ttcgtgggaa
     3241 cattggcaag ccgctcttgg ttttgatgtt acacacgccg atggtacagt cacaccgccg
     3301 ccattcccgg tgctacgctg gttatggaac tccatcaaag tggcaacaat tacctctatc
     3361 ggcattgtaa ccttatctac gacctgtgct tatgcatttg cccgaatgaa atttaaaggc
     3421 aaaaaaacca ttctacaagg tatgctgatt ttccaaatgt tcccggcagt gctttcctta
     3481 gttgccttat atgcgttgtt tgatcgtctt gggcagtatg taccgtttct tggtttaaac
     3541 acacacggtg gtgttatttt tgcctactta ggcggaattg ccttacacgt ttggacaatc
     3601 aaaggctact ttgaaacgat tgatggttca ttggaagaag ccgcttccct tgatggtgca
     3661 accccgtggc aagcgttccg attaatctta ttaccgcttt cagtgccgat tttggcggta
     3721 gttttcattc tttcatttat tgccgccatt accgaagtac cggttgcctc actcctattg
     3781 cgtgatgtaa acagctacac cctagccgtt ggtatgcaac aatacctcta tccgcaaaac
     3841 tatttatggg gtgacttcgc agcagcggcg gtcttatccg caattccgat tacgctggtc
     3901 ttcttattgg cacaacgctg gttaatcggt ggtttaactg ccggcggcgt gaaaggctag
     3961 tttgcaaaaa attctctaaa aacgaccgct tgtcgagctg atactgcggt cgtcaatctt
     4021 tattagggct gtctatgaaa aatgcgttac cttatctctg cttattctat acctcaagcc
     4081 tttttgccca atcttggcaa cattcccatt tttctgattt taacgatgat ctcgaaaaac
     4141 atttttttca atcccaagtt caattggaaa aagggagcta tccgcttagg ttcaaatttg
     4201 gcgaacaatg ttatcaaccc caacaagcga taaaattaaa tcaatcggtt gcgttagtcc
     4261 cttgtataaa tgaagctcca caactgcggt tgtttcgtca aggcaactat ctggcacaaa
     4321 ttgatctgcg aagcggcact ccgacaccca caatcagcgt agaacagcag ctacagcaca
     4381 atgaagcgat gtttcaaagt tgcccgaatt gggataaaaa accgattgaa attgatgttt
     4441 cttccacttt tgccgaaggc gaatcggtta gcgattttta ttcaggcaac accgctatcg
     4501 ttaaaaacgg caaagtcacc ttaatgccca atgaaaacgc aagcggtctg attttgctgg
     4561 aaaaaagcaa taccgaaaat acggcaacgt ttgattggaa aaatgccacc gtctattttg
     4621 tgctgaccga ccgcttttat aacggcgatc cgaccaatga ccacagctac caccgccaaa
     4681 aagacggaat gcaagaaatc ggcacctttc acggtggtga tttaaaagga ttaaccgaaa
     4741 agctcgatta cctacaacaa cttggggtaa acgtattgtg gattagctct ccgttagaac
     4801 aaatgcacgg ctgggttggc ggcggtgata agggggattt tccgcattat ggctatcacg
     4861 gttactatca tttagattgg acgaaattag atgccaatat gggtactgaa gacgatttaa
     4921 gtcgctttgt tcaacaagcc caccagcgtg gtattcgggt gctatttgat gtggtgatga
     4981 accataccgg ctacgcgacg cttgccgata tgcaggagtt taactttggc ggattttact
     5041 taacaccgga cgaaattagc cgcacattag gcgaaaaatg gacacactgg aagccgaaat
     5101 caggacaaaa ttggcacagt ttcaatgatt tcattcgctt taacgataat caggcttggc
     5161 aaaattggtg gggaaaagaa tgggttagag ctgatattgc cgattacgac agccctaaat
     5221 ttgatgattt aaaaatgtcg ctcgctgccc ttcctgatct aaaaaccgag agcgaacaag
     5281 cggtcaaatt acctgaattt tttgcaaata aaaataccaa tgccaaaaca ttaccaaacg
     5341 ccaaagtgcg tgattattta attggttggt tatccgattg ggtcagaaaa tatggcattg
     5401 acggttttcg ggttgatacc gccaaacacg ttgaaaaatc cacgtggctg gtattaaaac
     5461 aatcggcaca acaagcctta aacgaatggc aaaaagccaa tccgaacgcc ggttttaacg
     5521 atcgcttttg gatgacagga gaagcgtggg gacacggcgt gttcaaaagt gactattatc
     5581 aaaacggttt tgatgcaatg atcaatttcg attttcaaga tcaggctaaa caagggttaa
     5641 attgctttgc ccatattgag ccgatttatg ccgaaatgaa tcgcaaatta agcgacttca
     5701 atgtgttgag ctatttatct tcacacgata ctcgattatt tttccattcg gatagtgaac
     5761 aaaatatcga aaaacacaaa accgccgcaa atttgctgtt gctttcaccc ggtgcagtgc
     5821 aaatctatta tggtgatgaa agtgggcgga catttggtgc gaccggttca gacctgatac
     5881 aaggcacccg gtcggatatg aattggcaag agttgcagca agatcctcag aaacaggcat
     5941 tgcatcaaca ttggcaaaaa ttgacacaat tccgccaacg ccatccggct attggcgcag
     6001 gtgtccatca actcatcaaa aatgatagct actttgcatt taagcgtgtg ttgaatgaag
     6061 ataaagtgat ggtggtttgg gccggaaatt aaatctactt tagcttcaag ttttctctct
     6121 tctggggaaa acttgaagcc ggttcgggga aacaagttgc aaaaaattac taaaaataga
     6181 ccgcttgttc cccatgagct tgtgatcaat gataccccat caacatcagc agttctttcg
     6241 tatagctgat tgcttcgtta cgattcgtca caccgatttt ctgataaaga tttcggatat
     6301 gggttttaat cgtggttgca gccacttgca attcatctga aatttgttca ttgctatatc
     6361 cggagtaaat caaacccaat acttgccatt cccgctgagt gagcggacta atttttaaga
     6421 gttccggcac tttcggattt ttcaataatt gattcacaaa gttttcatca aaatgggcaa
     6481 atttttggcg ataaaattga ttgatgttgc gtaaaataaa ctgtgctttg tgcaatacca
     6541 gttcatctag cacattaagt tgcaataaat ggcggatctg ctgtgccatt acatcccctt
     6601 caatcacaaa cgcactgata aaattcgttt ggcgggtgag ttttagtgcc tgaattaaat
     6661 cttgctgagc caattctttc tcgccttgta aaaaataaag ccggttacga acaattaatg
     6721 cgcgatttaa atcgctaatc agagaaaatt tttcagccgt ttcaattaaa ttatctaaaa
     6781 tggctttcgc ctcgtgatat tcctcaagca agattcgaga ccgtgcaata tttcgccatt
     6841 gcacttgggt aaaatgattt ttatctgaaa ccggtgaggg gttttgaatt aaccagttcc
     6901 tcaccgcaga aacgtcattg gtcatctgcc aataaaatat acgtacctga tcggcgttgg
     6961 caatccaatc gtggtgataa gaatgagaat gttctaattg ttcgacctcg cctaataatc
     7021 gggcagtgtt atccaaattg cctcgagcca acgagatctt cgtgagtaaa gttaagcatt
     7081 gtaacttatc ttcaaattta gataaggcat tcattccggc aactgccatt gattcagctt
     7141 tatctaagtt ataccattcc cataaaatct tgcctttaga acgtagcaaa aattcataca
     7201 tcggcacttt ttgtagatgg ttttctttca caaactcgct tgctttatct aacatttcat
     7261 aggctgcttg tgaaaaccct tgggcaagca aaatttcaga ttgttgaagc tgtgaccaca
     7321 aaatattgtg gtaagtatga tgctgacgtg ccatacgctc tgctttttgc aacattgcta
     7381 atgcttctgc cagattgccg tggcaatgat gagcttcgcc aataatagac gttgcaacaa
     7441 tatgagcata ataggcattt tctgaaagat cgtttaacgc ttcagacgct aattgaagtg
     7501 cggtattctc atcaccggaa ttgatagcaa cttgtgcccg tagcacatta aattccgcat
     7561 tggcagtttt gctcaattca attttatttt cagttaatgc acgagaaaaa tccgctaaaa
     7621 taccacctac ttcaatatgg cggtgctggc tttgtaccaa ccaggctttg agtaacacca
     7681 aattagtatg ttcggttaaa tgggcgtaat ctaagcaatt taagctctct tccaataatt
     7741 tgagttctcc ttgatgaaac accgtccaag cgtgtttgct caaaatattg agtagtaatc
     7801 cggtatccga taactgcatt gcgtgatgta aggcttctgt aacatagcct aattgtaacc
     7861 acgcattcgc cgccttttga tgcagttgcg gtaattcagc atataactca ttttggcaac
     7921 ataaatttaa aaaggaagca aaaagcggat ggaatttcca ccagctatca tcactactct
     7981 gccacttact gtttgccatt tgttgtaaaa acaaaccttg cttttcgaga gactctaatt
     8041 tggtgcgact gttttgctcc cctgtaacag cctgcactaa agactcattc attgaatgca
     8101 aaaccgaaca acgcaaaata aacaaacgag tctcttcatc gactttatta agcacttcat
     8161 cggctaaata ttcgttaata tgaaaatggt ttgatttggc taaacgtttg gcaatatctt
     8221 gcagtggtac ttgtagcgtg tgtgatttat tttttgcaaa aaggctaatt agctgaaggg
     8281 ctgtgggcca cccttcaact tcgttacaca gtgcgataac atcttgctga tttaactccg
     8341 aacctaatct tgcttgaaaa aaagcggtac tttcttggtg atcaaacatt aactggttaa
     8401 tatcaatttc caataattgt tcttgcactc ttaaactggc aatacttaac gggggaattg
     8461 atcgagaaat caagataagc gtcatattag acggttgatg tttaatccaa tattttaatg
     8521 cttcgtgaat ttcatcattt tcaataatat gataatcatc aatagcaaga taaaaatgct
     8581 ctgaaaatgc cgcggcttta atcaataatt gattaaataa agctaataaa ttagctttac
     8641 gattttcctc taacaatacg tctatttctt cattgattgc tgaatgcaat gccgcactaa
     8701 aataagtggc aaaacgatct gaattattat cgccttcatc taacgcatac cagccgatat
     8761 tctttttatt ttcaatccat tgggaaacca gagtggtttt gccataacct gccggtgcat
     8821 ttatcaatac gaccggataa ctgcaagatt tgtctaattc ttgaattaaa cgttttcgag
     8881 gaatgctgtt ttgtaatcga aaagaacaaa caagttttga tggtattaac ataataaatt
     8941 caattttctc tcataacaaa atagggatga tatatccacc atttgtgttc tcactacttc
     9001 cttataatca taccatacaa gatgattatt gggatgattt gattttattt ggattttaca
     9061 taatagcgac attcatcttt aaccgggggt ttcctatgac atttaattct attgtagaaa
     9121 aatactgtcg ctactttgat gtgacagatc caaaacactt taccttacag cagtggtatc
     9181 aagttgttgc tgaaggttcc cttgagctaa tttatagcca gcctgcaacc aaaccgacag
     9241 aatcacgcca cgtgaactac ctctcaatgg agttcttaat cggtcggtta accggcaata
     9301 atttaatgaa tttaggttat tacgaacaaa ttcgtgacta tcttaaacaa tatcaagttg
     9361 aattagtaga tgttttagaa caagagcgtg atccggcatt gggtaatggc ggattaggtc
     9421 gtcttgctgc ctgcttttta gattcgatgg caactgtcgg acaaaatgca accggctatg
     9481 ggcttcatta tcaatatggt ctgtttaaac aaagcttcaa agagggaatg cagaaagaag
     9541 cagcggatac ttgggctcgt gacagctacc cgtggcatcg ttttaatccg tctaaaaccc
     9601 gccatatcgg gtttggtgga aaaatcaaac atatccaagc cgatcaatat gaatggcagc
     9661 ctaaattgac cattcaaggc aaagcctttg acttgccgat tgtaggttat aaaaacaaca
     9721 tcattcaacc attacgttta tggcaagcag atagtgacca atcttttgat tttgatgcct
     9781 ttaatgaggg taaattttta agtgccgata aaacgattgt caatgcagcc gcactaactc
     9841 aagtgctgta tccaaacgac aaccatcaag ccggacaaaa attacgctta atgcagcaat
     9901 acttccattg tgcctgctcg gttgccgata ttttagatcg tcacattagc gaaggttatt
     9961 cgttagaaga tttagcaaaa cgccaagtta ttcaactcaa tgatacccat ccaacgctcg
    10021 ctatacctga attgatgcgt gtactgttag atgaacatca attcacttgg gagcaggctt
    10081 gggatatttg cagcaatacc tttgcttaca ctaaccatac cttattacct gaagcacttg
    10141 aacaatggga tcaacgccta tttaagcagc tattacctcg ccattatcaa attgtggaaa
    10201 aaatcaatga tattttccac gacaaagtac aggcagaatt tggtgatgat gcaaaaattt
    10261 gggaaaaatt agccgtgcta tttgattacc gtgtgcgtat ggcaaacctc tgcgttgtca
    10321 cctgcttccg tgtaaacggc gttgcggaaa ttcactccga tctcttagtg accgatctat
    10381 tcccggaata ccacaaatta ttcccaacaa aattctgcaa tgttactaac ggcatcacac
    10441 ctcgccgctg gattcgacaa gctaatccac aattaagtga tttattggat cgtaccttaa
    10501 aacaagattg gacacacaat ctggaattat taaaaggcgt tgaacaatat gcggatgaca
    10561 ccggtttccg tgaagaatat caacgcatta aacgcaataa caaagtggtt ttagccaatg
    10621 aaatccataa aacattaggg ttaaatgtca atcctaatgc gatttttgat gtgcaaatta
    10681 agcgtttcca cgaatataaa cgccaacatt tgaatttgtt aaacattatt gcgacctatc
    10741 aatcgctaaa agctaacccg aaccaagact acacaccacg tctattccta ttctcgggca
    10801 aagctgcacc gggttattat ttagcaaaaa atattattca cgctattaat aatgtggcag
    10861 atattattaa taacgacaag caggtaaatg atcgactaca agtggcattc ttaccggatt
    10921 atcgagtcag tcttgcggaa aaaattattc cagcagctga tgtatcagaa cagatttcaa
    10981 tggcaggtaa agaagcctcc ggtaccggta atatgaaatt ggcactcaac ggcgcattaa
    11041 ccttaggtac gctagacggt gccaatgttg aaattgctga tatggtcggt gaggagaatg
    11101 tctttatctt cggtcacacc gtagaaagcg tgagggaatt attagcaaaa ggctaccaac
    11161 cgaaagccta ctataaccaa gatccgattc tgaaaaatgc ggttgatttc ttagccacag
    11221 gaaaagcctc taacggagat aaagaggcat tcaagctgat gttagatagc ctgttggaac
    11281 gagatccttt cttagtcttt gccgactttg atagctatcg ccaagcacaa gagaaaattg
    11341 gtgtagccta tttagatcaa gaaacttggc tgcgtagtgc aattttaaat actgctcgtt
    11401 tgggtgcatt tagctcagac cgctcaatta aagattatca acaacgtatt tggttgaata
    11461 gataataaat tcgggcgact tctacaataa aggaagtcgc c
//
LOCUS       DANLXS010000071        11330 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.071, whole genome shotgun sequence.
ACCESSION   DANLXS010000071 DANLXS010000000
VERSION     DANLXS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11330
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.071"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            330..1058
                     /gene="ttrB"
                     /locus_tag="PXD71_001371"
     CDS             330..1058
                     /gene="ttrB"
                     /locus_tag="PXD71_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrB"
                     /protein_id="HDL6291488.1"
                     /translation="MDLSKRSFLKELSALTVGASFVPLQSAQAFMPAWREGDENKRYA
                     MLIDLRKCIGCQACTVSCSVENATPLHSFRTTVRQYEITNGTQVVNNVVLPRLCNHCD
                     QPPCVPVCPVQATFQRKDGVMVINNEQCIGCGYCVQACPYDARFINEETKTADKCTFC
                     THRLEVGLLPACVESCVGGARVIGDLNDPKSQISQLYQAHKDDLKVLKPEAGTVPHVF
                     YVGLDDDAFVSKIDGQPMLWTGEA"
     gene            1060..2091
                     /gene="nrfD"
                     /locus_tag="PXD71_001372"
     CDS             1060..2091
                     /gene="nrfD"
                     /locus_tag="PXD71_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HDL6291489.1"
                     /translation="MIREVLVEPQHIVWLPWIVHYFFFVGVAATAVFTAVLFAKKSLK
                     PTACELAAVTVALIGSIVAPVALTADLHQPSRILHFYTDFAWRSPMAWGAIILPLFSV
                     AVAGYFVLALAHHTRPNLPKWLAWLQLPILQNQGLLWAFRLFATLTAIGIIGYTVLET
                     YQTGTRTLWHSAWLLPIMLFSAWAVALGLTQAVCKMLFHTQTNTGLWTKLCLILTALS
                     LVGLAFSSPQATQDFAQLFNGSITAYLVGIFWLIALICNFSAKNHRLQWLGVLALIAF
                     GWLLRWVLVIQVQTIAKTNALQNPYHFDWTAVDGGLGIVSIFGLTVLVSIVVWQLILI
                     ICGGRKGEQ"
     gene            2081..5176
                     /gene="ttrA"
                     /locus_tag="PXD71_001373"
     CDS             2081..5176
                     /gene="ttrA"
                     /locus_tag="PXD71_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrA"
                     /protein_id="HDL6291490.1"
                     /translation="MNNKRRNLLKGGLALGGAAAFGAGYSPKVSEIAKGVMHGTSGEK
                     TRDAIHGNSLTPEYQVKDGKLLSNPEQIVCNTQCMGCWTQCGIRARVDLNTNQVIRIS
                     GNPYHPLSSDKTLPFGMPIAKAELLLAGESGIENRSTACARGAAFLEGVNSPYRITQP
                     LKRVGKRGEGKWQTISFEQLVEEIINGGNLFGEGEVEGLKTIRNLVDPINPKQPDLGM
                     KANQLLVTFAGPEGRQPILQRFAQKSFGTINFSNHGAYCGAAYRAGSGAFMKDFDNQQ
                     HAKPDWDHAEFILFIGTSPAQSGNPFKRQARQLAKRRPDDNFSYVVVSPRLEMTSSLA
                     TQNNHWVPIKPEGDLALVMGMLRWIIENERFNADYLSRPSLAAMENAGHVSYANASHL
                     IICEPNHPKFGQALRISDLQDVQLDPEEKVEENLIVVKDQASGELISAKDCLQAVIFV
                     EQIAKLLDGSEVLVKSSMQLLKEATQEKTLAEYSDICGVPVQTIENLAKKFTSHGHKA
                     NAITHGGTMHSTGFYTSWAILLLNAMVGNLNKKGGMAVAAGKFKDFGDGPRYNLANFP
                     NEVKPKGTNLARSKKAYQNSSEFKQKVTKGENPYPAQGAWYPFTGGQTTEMFTSMVQG
                     YPYFAKAWINHMANPVYGLTAMNPIALEKLKDPKILPLFVSIDAFMNETTALTDYIVP
                     DTHNFESWGFSTAWGGVPQRTSTARHPIVPSKNAKTAEGDTICMENFVIAVAKKMGLP
                     GFGENAIADLEGNRYPLHRTEDYFLRAAANIAFAGEKPAPDASEQDLLLTGVQRIMPK
                     IEQTLKAEERLKVANVYCKGGRFAPHESAWKEENMEFQWKNCLQIWNENVYKAQHHSN
                     GEHYWGCPRYMSPRFADGSTLEQHYPQSEWGFKLISFKSNIMSSITAPLLRLLSIKPE
                     GLVAMNRLDAEEKGIKHGDVVKLSTPTAGLTVQIVVLDGIARGTIGIEHGYGHKQIGA
                     SSYTIDGVEIPANPMIAKGINLNDLGIIDHTKAVKSPWVDWVCGSAVRNGIPAKVEKL
                     A"
     gene            5317..6162
                     /locus_tag="PXD71_001374"
     CDS             5317..6162
                     /locus_tag="PXD71_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6291491.1"
                     /translation="MTFVKKTALSLIATALFATSSFAAEQSQYQHIRNATAKIEYAGK
                     TFLVDPYLAEKDRYLPPNAKKRNPTLDMTVSIDEVLNGVDAVIVTHTHHAHWDDVAQQ
                     RVPKNLPIFVQNAGDARLIRSQGFTDVRVVGKDTPFEGVTLTRTRGQHGTDAMYANPK
                     IAEMVGDAMDVVMKAEGKPTVYLVGDTIWNHEIEHSFNTYKPDFVVLNAGCAKLPNFE
                     GSPIMCTEDVSKAHKIAPQAKILTVHMDALPQTSISSDQMRDYVQKEGLNRVTVPKNG
                     DVIKF"
     gene            complement(6275..7672)
                     /locus_tag="PXD71_001375"
     CDS             complement(6275..7672)
                     /locus_tag="PXD71_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL6291492.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
     gene            complement(7762..8385)
                     /gene="gmk"
                     /locus_tag="PXD71_001376"
     CDS             complement(7762..8385)
                     /gene="gmk"
                     /locus_tag="PXD71_001376"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL6291493.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            complement(8498..8971)
                     /gene="gpt"
                     /locus_tag="PXD71_001377"
     CDS             complement(8498..8971)
                     /gene="gpt"
                     /locus_tag="PXD71_001377"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL6291494.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            complement(9117..10526)
                     /locus_tag="PXD71_001378"
     CDS             complement(9117..10526)
                     /locus_tag="PXD71_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6291495.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            complement(10722..>11330)
                     /locus_tag="PXD71_001379"
     CDS             complement(10722..>11330)
                     /locus_tag="PXD71_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL6291496.1"
                     /translation="AEKEVDPIAIIKAKGIDDTNYGLKLGNSKETIEGKVTVRTQSYL
                     YNQPYSVVTAQAVQRNGWENGNYIDKVDGTVTVKGLKTEKLPTEGTATYSGKAFNHDG
                     AKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLIVLEEGTINNTQISSTVHGNNPLNLSG
                     KYTVEFFGKNAEEIAGEIQTKANLTGEVSNFGLAGTRGDIQK"
BASE COUNT         3074 a         2632 c         2592 g         3032 t
ORIGIN      
        1 ataaatagta ttgttatatc taaattaaaa gcaggatgga aaattagaaa aggaaagaaa
       61 cataatatat taattgcccc aaattctagg agattagcga tacctagtac cccgagtgat
      121 attaatgcgt ataaaaactt tagatctaaa ctaaaccatc ttattgcttt ttaattagta
      181 tcaatgccct attatctact tgtatagggc atttcccttc atccttgacc caaatcaact
      241 ttccccttga tatgtggaaa accacaatac cttgccgttc cctctccgcc ttagaatacc
      301 tttgttattt ttaaatttgt gggggaaata tggatttaag caaacgttcc tttttgaaag
      361 agctttccgc cttgacggtg ggggcgagtt ttgtgccgtt gcaatcggcc caggcgttta
      421 tgccggcttg gcgtgagggc gatgagaaca aacgctatgc gatgctgatt gatttgcgta
      481 aatgtatcgg ctgtcaagcg tgtaccgtga gctgttcggt ggagaacgcc acgccgttgc
      541 acagtttccg caccacggtg cgtcaatatg aaatcaccaa cggcacgcag gtggtgaaca
      601 atgtggtgtt gcctcgtttg tgtaaccatt gcgatcaacc gccgtgtgtg ccggtctgcc
      661 cggtgcaggc aacctttcaa cgtaaagacg gtgtgatggt gattaacaat gagcagtgca
      721 tcggctgtgg ttactgcgtg caagcctgcc cgtatgatgc ccgttttatc aatgaagaga
      781 ccaaaaccgc cgataaatgc accttctgca ctcaccgttt ggaggtaggt ctgctgccgg
      841 cgtgcgtgga aagttgcgtg ggcggggcaa gggtgattgg cgatttgaac gacccgaaaa
      901 gtcaaatttc ccaactttat caagcccaca aagatgattt gaaagtcctg aaaccggagg
      961 ccggcaccgt gccacacgtt ttctatgtgg ggctagacga tgatgccttt gtgagcaaaa
     1021 ttgacgggca accaatgctc tggacggggg aggcgtaaaa tgattcgtga agtattagtg
     1081 gaaccgcaac atatcgtctg gttgccgtgg attgtgcatt atttcttctt cgtaggcgtg
     1141 gcggcaacgg cagtgtttac cgccgtgctg tttgcaaaaa aatcactgaa accgaccgct
     1201 tgcgagctgg cggcggtgac ggtagccttg attggctcga ttgtcgcccc tgtggcattg
     1261 actgccgacc tgcaccaacc gagccgtatt ctgcatttct acaccgattt tgcgtggcga
     1321 tctccaatgg cgtggggagc gattatcctg cccctgttca gcgtggcggt agcgggttat
     1381 tttgtgctgg cattggctca ccatacccgg ccgaacctgc cgaaatggtt agcgtggcta
     1441 cagttgccga tactgcaaaa tcaaggcttg ctttgggcat tccgcctgtt tgccacactc
     1501 accgctatcg gcattatcgg ctacaccgtg cttgaaacct accaaaccgg cacaagaacc
     1561 ttatggcaca gtgcgtggct gctgccgatt atgctctttt ctgcgtgggc ggtggcattg
     1621 gggcttaccc aagcggtctg caaaatgctg ttccacaccc aaaccaacac ggggctatgg
     1681 acaaaactct gcctgatttt aaccgcactt tcgcttgtcg gactagcatt cagtagtcca
     1741 caagccacac aagattttgc ccagctcttc aatggctcaa tcaccgctta ccttgtcggc
     1801 attttctggc taattgccct catttgcaat ttttctgcca aaaaccaccg cttgcaatgg
     1861 ctcggcgtgc ttgccctcat cgctttcggc tggctactcc gctgggtact ggtgatccaa
     1921 gtccaaacca ttgccaaaac caatgccttg caaaatccat atcatttcga ttggacagcc
     1981 gtcgatggtg gtttggggat tgtctcgatt tttgggctaa cggtgctagt aagtatcgtg
     2041 gtatggcaac tgattttaat aatttgtggg ggaagaaaag gtgaacaata aacgcagaaa
     2101 tctgttgaaa ggcgggctgg cacttggtgg ggcggcggcg tttggggccg ggtattcgcc
     2161 gaaggtgtcg gagattgcca aaggggtgat gcacggcaca tcgggcgaga aaacccgtga
     2221 tgcaattcac ggtaattcgc tcacgccgga gtatcaggta aaagacggca aactgctcag
     2281 caatcctgag caaattgtgt gcaatacaca gtgtatgggc tgttggacgc agtgtggcat
     2341 tcgggcgagg gtggatttga acaccaacca agtgattcgc atttcgggca atccgtatca
     2401 cccgctttcc tccgacaaaa ccctgccgtt cggtatgccg attgccaagg cggaactgtt
     2461 gctggcgggc gaaagtggca ttgaaaatcg ctccaccgcc tgtgctagag gggcggcgtt
     2521 cttggagggg gtgaacagcc cgtatcggat tacccaaccg ttgaaacggg tcggaaaacg
     2581 gggcgaaggc aagtggcaga ccatcagctt tgagcagttg gtggaggaaa tcatcaatgg
     2641 tggcaaccta tttggtgaag gcgaagtgga gggcttgaag acgatccgca atctggtcga
     2701 tccgattaac ccgaaacagc ctgatttggg aatgaaagca aaccagcttt tagttacttt
     2761 tgccggcccc gaaggtcgtc agccgatttt gcaacgcttt gcccaaaaga gcttcggcac
     2821 gatcaacttt tccaaccacg gggcttattg tggcgctgcg taccgtgccg gttcgggtgc
     2881 ctttatgaaa gattttgaca accaacaaca cgccaagccg gactgggatc acgctgaatt
     2941 tattctgttt atcggcacgt ctccggcaca gtcgggcaat ccgtttaagc gtcaggctcg
     3001 ccagcttgcc aaacgccgcc ctgatgacaa tttcagctat gtggtggttt cccctcgttt
     3061 ggagatgacc tcctccctcg ccacccaaaa caaccattgg gtgccgatta agcccgaggg
     3121 cgatttggcg ttggtaatgg gaatgttgcg ttggattatc gaaaacgagc gtttcaatgc
     3181 tgactacctc tcccgcccaa gtctggcagc gatggaaaat gccggccacg tcagctatgc
     3241 caatgcgtca catctgatta tttgcgaacc gaaccacccg aaattcgggc aggcgttgcg
     3301 gattagcgat ctgcaagatg tgcaactcga ccccgaagag aaagtggaag aaaacctgat
     3361 tgtggtaaaa gatcaggcaa gcggcgaact catttcagca aaagattgct tacaagcggt
     3421 catttttgtc gaacaaattg caaaactgct tgatggctcg gaagtgttgg tgaaatcctc
     3481 aatgcagttg ctgaaagagg cgacacaaga gaaaaccctt gccgaatata gcgacatttg
     3541 tggcgtgccg gtgcaaacca ttgaaaactt agcgaaaaaa ttcacttcgc acggtcataa
     3601 agccaatgcc atcactcacg gcggcacgat gcacagcacc ggtttttata ccagttgggc
     3661 gattttgttg ctgaatgcaa tggtcggtaa cctcaacaaa aaaggcggaa tggcagtagc
     3721 cgcaggcaaa ttcaaagatt tcggggacgg gcctcgctac aatttagcga acttcccgaa
     3781 tgaagtgaag ccaaaaggca cgaaccttgc ccgcagtaaa aaggcgtacc aaaattcaag
     3841 cgaatttaag cagaaagtga cgaagggcga aaatccgtat ccggcacaag gggcgtggta
     3901 tccgttcacc ggcgggcaaa ctaccgagat gtttacctca atggtgcagg gctatccata
     3961 ctttgcgaaa gcgtggatca accatatggc gaaccccgtt tacggcttaa ccgctatgaa
     4021 cccgattgcg ttggagaaat taaaagatcc gaaaattcta ccgctctttg tctctattga
     4081 tgcttttatg aacgaaacca ctgcactgac ggactacatc gtgccggaca cccacaactt
     4141 tgagagctgg ggcttttcaa cggcgtgggg cggtgtgcct caacgcacca gcaccgcacg
     4201 ccacccgatt gtgccgtcca aaaacgccaa aactgccgaa ggcgacacca tctgtatgga
     4261 gaatttcgtg attgcggtgg cgaaaaaaat gggcttgccg ggctttggcg aaaatgcgat
     4321 tgccgattta gaaggcaacc gctatccgct ccaccgtacc gaagattact tcctacgggc
     4381 ggcagcgaac attgcctttg ccggtgagaa acctgcaccg gacgccagcg agcaagatct
     4441 gctgctcacc ggcgtgcaac ggattatgcc gaaaattgaa caaacgctaa aagccgaaga
     4501 gcgattaaaa gtggcgaatg tgtattgcaa aggcggacgt tttgccccac acgaaagtgc
     4561 gtggaaagag gagaatatgg agttccagtg gaaaaactgc ctgcaaattt ggaacgaaaa
     4621 cgtgtataaa gcccagcacc acagcaacgg tgaacactat tggggctgcc ctcgttatat
     4681 gtcgccacgt tttgcggacg gctccacgct ggagcagcac tatccgcagt cggaatgggg
     4741 ctttaaactg atttcgttta aatcgaacat tatgagcagt attaccgccc cactgttgcg
     4801 tctgctctcg atcaagcctg agggcttagt ggcgatgaac cgtttagatg cggaagaaaa
     4861 aggcattaaa cacggcgatg tggtgaaact cagcacccca accgccggct tgacggtgca
     4921 gattgtggtg ttggacggta tcgcccgagg cacaatcggc atcgaacacg gctacggtca
     4981 taaacagatt ggggcaagtt cctacaccat tgacggcgtg gaaattccag ccaacccgat
     5041 gatcgccaaa gggattaacc tcaacgacct cggcattatc gaccacacca aagcggtcaa
     5101 atccccgtgg gtagattggg tctgtggctc agcggtgaga aatggcattc cggcgaaggt
     5161 agagaaatta gcctagccga acaagcggtt ggttttgcag aaaaatgtgc aaaatcgacc
     5221 gcttttattg atgtaaggga cactgttcct tatgcaaacc agcattgcag agcgtaaaaa
     5281 atcatactct gcattttaat ttattttgga gaacctatga cttttgtcaa aaaaacagca
     5341 ctgagcctga ttgcaacggc acttttcgct acatcaagtt ttgccgctga gcagagccaa
     5401 taccaacata ttcgtaatgc aacggcgaaa attgaatatg ccggcaagac gtttttggtt
     5461 gatccgtatt tagcggagaa agatcgctac ttaccgccga atgcgaaaaa acgtaatccg
     5521 accttggata tgactgtgag cattgatgaa gtgttaaatg gcgtggatgc ggtgatcgta
     5581 actcacactc accacgctca ctgggacgat gtagcacaac aacgtgtgcc gaaaaatctg
     5641 ccgatttttg tgcagaatgc cggtgatgcc cgtttaatcc gttcacaagg ctttaccgat
     5701 gtgcgtgtgg tcggcaaaga taccccgttt gaaggcgtaa ccttaacccg aactcgtggc
     5761 cagcacggca ccgatgcgat gtatgcgaac ccgaaaattg ccgaaatggt cggcgatgcg
     5821 atggacgtag tgatgaaagc ggaaggcaaa ccgaccgtct atttggtggg agataccatt
     5881 tggaaccacg aaatcgaaca ttcgttcaac acttacaaac cagactttgt ggtgctgaat
     5941 gccggctgtg ccaaactacc aaactttgaa ggcagcccga ttatgtgtac cgaagatgtt
     6001 tccaaagcac acaaaatcgc tccacaagcg aaaatcttaa ctgtgcatat ggacgccctg
     6061 ccacaaacct caatcagcag tgatcagatg cgtgattatg tacaaaaaga gggattaaat
     6121 cgtgtaactg tgccgaaaaa cggtgatgtg attaagttct agtagctaaa ctaacattac
     6181 caaaatggtt taagtagcaa taaatagcga agcgggttaa gccatcaagc cttaacccac
     6241 ttttaccaac catagtatat agttttagat ataattatct tctgtattta cctgccattg
     6301 cttggtaaac cgcattctcg ttggtcaaaa tcgcatattt tgcatttaaa atcgccaact
     6361 gagaattaag ctcggtattc agtgcggtaa tccactcacg gaactccgac accccttgtt
     6421 cataacggtt tttatagtat gaactgatac gtttattttt cgcattggta tcttgaagga
     6481 tttttaacga gcttctcgac tgcgtataac tgtaataata acggtcaatt tcatttaagg
     6541 ctgaggtaat tttttgctca tagtttaaac gggcagtcac ataatccgtt tcagaaattt
     6601 ttacgttatt tttaacccga ttccaatcta agaacggtaa atccaattta attaagccgt
     6661 tacccacagg gttatctgcc acattgccga tattggttgc actaccggta atagaaccac
     6721 cgatggttaa ggttgggaac cagcttttct cggttgcggt taaggtttta aatgcacttt
     6781 gtaagcgttg taacgagcct actacatcag ggcgattagc aatggctgac actggcacat
     6841 ttaaatctac cccttgcaat ttcacgctca taatattcgg gtaacgaatg ttaagcggag
     6901 tatttggctg aacatttaac aaattgcgta atgtttgctc ggcaattttt tggttggttt
     6961 tcagatcaat tacactattt ctggcgttta agaccgcttg ttgagcctga tcaaccgcta
     7021 aactatcaat cgcaccggca ttcattttgt tagttaaaat acggctgatt tgctcataat
     7081 ggcgaatgct ctcttcggta atacgaatcg catcacgata gtaagctaac tggtagtaag
     7141 tagtcacaac cgcattaata atagataaac gggtagcttt taagtcttca atggtggctt
     7201 tatgcgtcca ttctgccgca ctggcggtat cggctaaacg tcgccataaa tccacagtat
     7261 aacttaaatt aaatgcagct tgagctgcgg ttgttgaaac accggtcgaa acattatttg
     7321 aagaagccga acctaaacct ttagccgcag aaacagaacc tgaaccacta aaggttggca
     7381 ctaaatttgc ccctactaaa ttagcgttat acaaggcttt attgactgca attccgcttt
     7441 tggctaaatc aaggttattt ttgatggcta aatcaaccac acggtttaat tccgcatccg
     7501 tatagccttg ccaccattgc tcgttaattt gatattgttt ggtaatttca tcatagcttt
     7561 gataggtttg ctgtgcttgt tgcaaagtcc cgtcattgct catattccct gtggtacaag
     7621 ccgttacggt tagagccaac gccacagaaa atgctaattt tgaaagtttc attacatttc
     7681 cttttgaaaa taaagagggg aaaatgggcg tattctaccc aaaaacagat tgcctttcca
     7741 gttaaatgaa caactgttca tttaattatt taacaaatca gtaatcaaat gctgatgacg
     7801 aatttgttgc gaacctaagc tcaaacgctc ggcaattaaa atcgctttta gctctgccaa
     7861 tgcctgttgg aaatcatcat taataatcac ataatcgaac tcattgtaat ggttcatttc
     7921 cgacaccgcc tctgccatac gtttggcaat ggtttcagcg gaatcttgcc cacgcccgat
     7981 taaacgtttt tccaattctg tttttgacgg tggcaaaata aaaacggttt tcacattcgg
     8041 cactttttcc cgaatttgtc tagccccttg ccagtcaata tccaaaaaga catcaatgcc
     8101 ctgctctagg ctacgttcaa tcatcggcaa tgatgtgccg taataattgc caaacacctc
     8161 cgcccattct aaaaaatacc cttgctcaat cagctgctta aactcatcgt gcgaagtgaa
     8221 ataataatgc acgccgtcct gttctgccgg acgaggctga cgggtagtgt gtgaaatcga
     8281 aagctgtact tccgaacggg gtaaatccgc caacaaggcg ttaattaagg aagattttcc
     8341 tgctccactt ggggcagaaa taatataaag attgcctaaa ctcattattt gtatatacct
     8401 atttgatttt gggaataggg cgaatgatga ttcgccccta catttcatag gaataaataa
     8461 aaaataatgg acaactttca ttgtccatta cacaaaatta tctacgtgcc aaaggctgaa
     8521 cgaaggtaat gcctaaatcc caaggctgct caatccacgt ttcttgtgga atatcaatca
     8581 cataatcatc aaccaacggt gcaccgtgcg gtttcgcaaa cacggttaca aaacgagctt
     8641 tcgggtacat tctgcgaatt tcttttgccg tgttgccggt atccactaaa tcatctacca
     8701 caataaagcc ttcgccatcg gtatcggctg cgtgtaacac atttaactca ccttggctat
     8761 cgtgatcgta gctggcaatg cacactgttt ctacgtggcg aatacccaac tcacgtgcta
     8821 aaacggctgc cggaaacaag ccgccacggc tcaccgcaat aatccctttc cactgtgtgg
     8881 caggtaataa attttctgcc aacttgcgag cgtgcatatg gaacatatcc cacgtcacga
     8941 catatttttc atttgtgtat ttttcactca tagaaacaac ctaatataaa attgggggct
     9001 accccgatat aaaaaattgc actaggataa cccgaaaatg cttttttcgc catcatttca
     9061 tttatttttt tgtgccggca gctacgcaat cgtatgctta gcacaaaaaa ccaacactaa
     9121 aatagctttt ctaaagtgaa aaagacttcc ttgttttggc gagagtaaaa atcagcaata
     9181 ttgctgtcaa ttttttgata acggaaattc agcttcggca caatgccctg ccaatgccaa
     9241 gtgcggttcc aaaccgctaa attggcttga tattccgtgt ctaaccgctt atagccaaac
     9301 aaatagtgct tgtcggcaaa atatcgcttg ccaaagccga tacttgcctg cgttgcccag
     9361 cgttcgcctt gaaaaaccgc ccccactcga gccagccact tattcgagct ttgtgctttt
     9421 tcccgtgcaa tttcacgcac gccgtctaaa tttgcaaaaa accgcccatt tttgaccgct
     9481 tgccactgca caccgccact gagttggtgc tgatagctgt tataacggct tgcggttagc
     9541 tcctcaaaat accgtttttg caaatgggta aaactccccg acaccaacca ttgcgaatta
     9601 aggtaacgca aaccgttgag tgttgcccca aattgaacat tataacggct actccccagc
     9661 caattttgct caataaaagg cgaaaaagtc agccgataag cgacggtgcg gtagctataa
     9721 ttcggcgaaa cggatacgga ttgctcacgg taatctcggt tatcccaata gtaaatcccg
     9781 ctataattga gcgttaaacc gacaaaatga ttgcctttca cgttgtgcaa tttactcaac
     9841 cctaagccat aacgcacgcc ttttgctgat tgtggcaaac tgtctgcatt tttgcggaaa
     9901 atccgcccat ttacattcac aatcggcgaa gatgaggcat tattcacgtt atctgtttgg
     9961 gtatattgca gataaaaatc gggctgccac gcctcatttt cagcaatttg gacttgagta
    10021 ttgcgggcaa ttttagcaag ttccggcggt aaatgggctt gaatagcctt aaattcctgc
    10081 tctgccgcac gaaattgctg atcttctgcc aaaatcaacg ccaaatcaaa acgagggtaa
    10141 accaaactcg ggggcttagc taacagttgg cgatagtgaa aaactgcggt ttgatgatct
    10201 tttttcgcat aggcaagtgc ggcttgcgca tagtgccaca acaacgggtc aaaattttca
    10261 gtttgcggat aacggcttaa aagcggttca agcgttgccc aatcttggct gagaatcgct
    10321 tggttaattt gctgttcaac attctgattg actgaatacg caggctgata agcggtcggt
    10381 ttttgtgaat tttttacaac attttgctca aatttaaccg cttgcggctg acgctcaaag
    10441 gaaatcgggt cagtgttgtg ttcaatcacc ttttctgccc atacaacggg gctgaacagc
    10501 gaaaataaaa ggggatattt tttcataaag ataaagaaaa ttggtagggg cggacctata
    10561 tgttcgcctc tacgggattt atcactctct ccccttgtgg gagagggtta ttagaagctg
    10621 taaattgaaa tctaaactat tgtgtcttat ggtatctaca agcaatatca agacaaatgc
    10681 cgtttaattt ttcgttaaac ggcattgaac gttagaaaag gttatttttg gatatcgcca
    10741 cgagtgccgg caagtccaaa attactcact tccccagtaa gatttgcttt tgtctgaatt
    10801 tcaccggcaa tttcctctgc atttttaccg aaaaattcga ctgtatattt tccagaaagg
    10861 tttaaaggat tattgccatg tactgttgaa gaaatctgag tattattaat cgtcccctct
    10921 tctaatacga ttaagtttcc aaattctccc tcaactctac ctgaaccggt acgcttagag
    10981 aaattaacat tataggttag atcaccacca ttaaaacctt tagctccatc gtggttaaat
    11041 gcttttcctg aatatgttgc agtaccctct gttggtaatt tctctgtttt caatccttta
    11101 actgtaactg ttccatcaac tttatcaata taattaccgt tttcccagcc atttctttga
    11161 actgcttgtg ctgttactac agaatatggc tgattatata aataactctg tgttctgact
    11221 gtgactttac cctcaatagt ttctttacta ttaccaagtt ttaagccgta atttgtatca
    11281 tcaatacctt ttgctttaat aatagctata ggatctactt ctttttctgc
//
LOCUS       DANLXS010000072        11304 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.072, whole genome shotgun sequence.
ACCESSION   DANLXS010000072 DANLXS010000000
VERSION     DANLXS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.072"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            152..370
                     /locus_tag="PXD71_001380"
     CDS             152..370
                     /locus_tag="PXD71_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291497.1"
                     /translation="MYQYYLAQVGNEQQKLIRGMPDNLLSFSVYEPEQEDWDNKFGPF
                     WADKILISGFDAYEEISEKEAIRFIKVH"
     gene            371..784
                     /locus_tag="PXD71_001381"
     CDS             371..784
                     /locus_tag="PXD71_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL6291498.1"
                     /translation="MCQAEQFARQLHYGQTDKAGKPYIEHLAFVAENISTKNEETEAV
                     AWLHDSVEDTQCSINDIRHIFGDVIADAVEAITKKQGESYFCYLDRVKSNPIAKVVKL
                     ADLTHNLCLERLPKITEKDLKRAEKYKSALEYLQA"
     gene            902..1636
                     /locus_tag="PXD71_001382"
     CDS             902..1636
                     /locus_tag="PXD71_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291499.1"
                     /translation="MKLKLDENGHVVVENGMPVYIHDDGKEIPFDALKATQKITQLNA
                     EAKQHREAKEKAEADLKAFSGIDDPKAALEALKTVANLDAKKLIDAGDAEKVKAEIIK
                     GYDEKLAVATAQAEKLQQQLHAELIGGSFARSKYAQEHLNIPSDVVQAFFGKHFSISD
                     DGKVVAKFADGGEIFSRTRPGEKADFEEALEALVSAYPNKDAILKPSGSSGAGAGGGS
                     RSSSMPKTLSDCKTDEERIAYLQANS"
     gene            1702..2649
                     /locus_tag="PXD71_001383"
     CDS             1702..2649
                     /locus_tag="PXD71_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291500.1"
                     /translation="MAFDLQVFNKQTQLALTETVDQAIEKFNAASGGAIILQNKPAEG
                     DFDIRASFKGIAGLVRRRNVYGTGTVDAKRLEQLLDVAVKVAAGTAPIEYQPSQYKWI
                     LLNPELAAIEIGTQLAKARVADMLNTAIAGTVAAISGNTAMTLDEKTAAPTFRILNKG
                     AAKMGDRAGSLKAWVLHSTTLHGLYDNALTNAENLFKYENVNVIRDPFGRVFVVTDSP
                     ALMASDGTFNTLGLVENAVVVSGNNDFDSVLVPKTGGENLSAVYQAEWTYNLGILGYK
                     WDMTAGGKSPNDTALGTPTNWDKSVTSNKDTAGVLVKTK"
     gene            2709..3059
                     /locus_tag="PXD71_001384"
     CDS             2709..3059
                     /locus_tag="PXD71_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291501.1"
                     /translation="MKRILYFTTDFSAKNIAFAKAHGVLMRNLNAYHPSDTLENADAV
                     CGNVPQAYQHLPLFELPESNQKSGQDLKTLKTEEIKEKLTELGIAFEANAKKETLLTL
                     LTQAVGRDGTNDAK"
     gene            3049..3486
                     /locus_tag="PXD71_001385"
     CDS             3049..3486
                     /locus_tag="PXD71_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291502.1"
                     /translation="MLNSYMTVEQANAYHDIRMSREAWNALSDQEKQQRLVSASDFLD
                     FNYRFSGEKADLTQEREFPRKGFEAIGIPKAVQFAVCELALQDNLTANQEAQMSSVRV
                     GPVSVNYDNSSPQNSVNRFEYVRQLLSTWLDRSSFGTVKLERS"
     gene            3486..3857
                     /locus_tag="PXD71_001386"
     CDS             3486..3857
                     /locus_tag="PXD71_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291503.1"
                     /translation="MADFYGNLGKISHSLIKQFGQPCSVTINEQGRYNPATGERKKGS
                     STVHAGYCLFDNLAFDFRNQQSVSVQQGDVMIYLTADCKPVLNAVIEANGEKWSVINV
                     QPIRPAATTMIYQVQGRKADG"
     gene            3850..4263
                     /locus_tag="PXD71_001387"
     CDS             3850..4263
                     /locus_tag="PXD71_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL6291504.1"
                     /translation="MGSFHAQIARFVEQTQQKADRALRVIALQTLSGVQKKSPVDTGQ
                     LRRSWTVALNGFPSSYNGSQTVLNSAKFGDMIVIATNKPYAPMLEYGLYPNPPKKPTG
                     KTKNGYSIQAPNGMVRITVQEMQAFIRNNPQLGVS"
     gene            4263..4655
                     /locus_tag="PXD71_001388"
     CDS             4263..4655
                     /locus_tag="PXD71_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291505.1"
                     /translation="MRPIIRSVLEQHLAELNPFNTVWEGIIDSPKLPYQTVSMTTSTN
                     ETATIGNTPKAKQSGFLQVTLYYPLDQGTKAIEERADEIFRHFFGQVWIEENVQILVQ
                     QPPLVASLYRLDNSIALPITIYFTAYEL"
     gene            4665..5687
                     /locus_tag="PXD71_001389"
     CDS             4665..5687
                     /locus_tag="PXD71_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291506.1"
                     /translation="MSILGNSQGTKRTVVFAKESSFGTKAAPTGAKIMPRVETSLNTN
                     FESYQSEEIRDDLQRSASVIGFEKVEGDIKGELAAGQWAAFFAAALRGSFTAQAKKPI
                     ITKTTDGTGEKNGKILIVPKTSHTSDSFTLEDVFKDINVSRQYLGCRVSKLSLDVQPN
                     GIASITVSFLGQRGEESTTAYFTNPTDLTQSGKLAGVKGSLQVNKRPVGLVTAFKLDI
                     DLGASSEPVLGAKYAPDVFIGTVAVSGSFTMYFQDKTMIDAVRNGTDLSLALRMDAEE
                     RTNTDYLTIILPGVKVTSNELDDGAKNIMQTLNFDAFPAVWDSASQIDNVLKVATTMI
                     VQDSLA"
     gene            5777..6178
                     /locus_tag="PXD71_001390"
     CDS             5777..6178
                     /locus_tag="PXD71_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291507.1"
                     /translation="MNLNALSKEKQLAEHRFELVHPVSGEPLGVFMSVVSAKSDKAQR
                     FAEKQLRQEQMRELENAKSRKPKFKGLGDMIAESRAMAIDRLTGWENVEWNEKPLEFS
                     DQNAELLLTECDWIVEQIFEHSNDLGKFLKS"
     gene            6193..6519
                     /locus_tag="PXD71_001391"
     CDS             6193..6519
                     /locus_tag="PXD71_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291508.1"
                     /translation="MYAEKTFKLDKKLKGSESTLREHLQAIEMQTGITPEELHNPEPS
                     PAVAYLLGWFFELAMSRQSGMSLNPISYTEIDAWSRLFARHLEQWEISVIKQLDMIYL
                     NVQQTE"
     gene            6521..6844
                     /locus_tag="PXD71_001392"
     CDS             6521..6844
                     /locus_tag="PXD71_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6291509.1"
                     /translation="MDTFNFQPNWGLNVQRKPEVMKIAFGDGYEQVAPKGLNHNLRTY
                     SAVFTGTEARIKQIEAFFERQGGYKAFLWTPYGGKEGKFRCEEWEVSISSGKWTLSAT
                     IREVL"
     gene            6931..7623
                     /locus_tag="PXD71_001393"
     CDS             6931..7623
                     /locus_tag="PXD71_001393"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291510.1"
                     /translation="MKKIFVSALLLTCSLSALGNSSYELIIDSMQDLEKHKPEILSIP
                     EADRNTFIRYMMRKELRIKLNQGRLADGLTVREAIQNQQAFEKDRNSKMQQEKQKKET
                     EKAEKEKAVTDFNQKFELSITGARKAKNALNQDILLVSLSLKNNSDKPIIAVQPDIQF
                     VLGEDKQTISSMRPKKFENGIPPNGSEIFEFGFDLDDMFALKAEKNIEKLRAVFQTAE
                     VLFADGTTEKLQ"
     gene            7687..8511
                     /locus_tag="PXD71_001394"
                     /pseudo
     CDS             7687..8511
                     /locus_tag="PXD71_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            10555..10713
                     /locus_tag="PXD71_001395"
                     /pseudo
     CDS             10555..10713
                     /locus_tag="PXD71_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            10721..>11304
                     /locus_tag="PXD71_001396"
     CDS             10721..>11304
                     /locus_tag="PXD71_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL6291511.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRNLKDKSGNAGRVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEASGFERNSSGPSNRPTLTLSNLFGLITSIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNAKIPSRIIMADTCTWIYRSAECGYTGK"
BASE COUNT         3553 a         2223 c         2659 g         2869 t
ORIGIN      
        1 tgaaaaacgg taaattgcac gagctctatg aaaaattcag agatctgaat atacctcttg
       61 caaaaaaatc agaaatttta cttgaaatgc agaagttaag tgctaatcta aaagagccaa
      121 agaacattga tttaatcaag gagaaaaaat aatgtatcag tattatttag ctcaagttgg
      181 caatgaacag caaaaactta ttcgtggtat gcctgacaat ctacttagtt tctcggttta
      241 tgagcctgaa caagaagatt gggataataa gttcggccca ttttgggcag acaagatctt
      301 gataagcgga tttgacgctt acgaggaaat ttcagaaaag gaagcaatcc gatttatcaa
      361 ggtacattaa atgtgtcaag ctgaacaatt cgctcgccaa ctgcattatg gacaaacaga
      421 taaagctgga aagccataca ttgagcatct tgcttttgtt gcagaaaaca ttagcacaaa
      481 aaatgaagag acagaagcag tagcttggtt gcacgacagt gtagaagata ctcagtgttc
      541 aattaatgat attcgtcata tatttggcga tgttattgct gatgcagttg aggctattac
      601 taaaaagcaa ggcgaaagtt atttttgcta cttggataga gtaaaatcaa atcctattgc
      661 taaggtggtt aagttagctg atttgactca taatctttgc cttgagcgat tgcccaagat
      721 cactgaaaag gatcttaaac gagcagaaaa atacaaatca gcattggaat atctacaagc
      781 ctagcctaaa acgctaggct tttttatcgc aattttttac cccaaactga ccgtttgtga
      841 gccatcacag gcggtttttt attatcgggc ggaagcccac cagcaatgag aggaagctca
      901 aatgaaatta aaattagacg aaaacggaca tgtagtagtt gaaaatggta tgcctgtgta
      961 tattcacgat gacggaaagg aaattccgtt cgatgcacta aaggcgacac agaaaatcac
     1021 acaactgaac gctgaggcaa agcaacatcg agaagctaaa gagaaagcgg aagccgactt
     1081 aaaagcattc tcagggattg acgatccgaa agctgcactc gaggcattaa agacggttgc
     1141 caatttagat gctaaaaagc tgattgatgc cggtgatgcg gaaaaagtca aagcggaaat
     1201 catcaaaggt tacgatgaga aattagctgt ggcaaccgct caagccgaaa aactgcaaca
     1261 gcagttacac gctgaattaa tcggtggttc gtttgctcgg tctaaatacg ctcaagaaca
     1321 cttgaatatt ccgtctgatg tcgtacaggc attcttcggt aagcatttta gtatttcgga
     1381 tgacggcaaa gtggtagcta aatttgctga cgggggcgaa atctttagtc gcacccgccc
     1441 gggcgaaaaa gcagattttg aagaggcatt agaagcttta gtaagtgctt atccaaataa
     1501 agacgctatt ttaaaaccaa gtggttcaag tggggcagga gcaggtggcg gatcacgcag
     1561 ttcatctatg ccgaaaacat tatcggactg caaaacagat gaagagcgta tcgcttatct
     1621 tcaagcaaat tcgtaaggtg caagaggttg cacctttttt attttcggtg caatcgcacc
     1681 actttttaga ggaaaatcaa aatggcattt gacctacagg tttttaacaa acaaacgcaa
     1741 ttagccttga ccgaaactgt cgatcaggct attgaaaaat tcaatgcagc atcaggcggt
     1801 gcaatcattt tacagaacaa gccagctgaa ggtgattttg atatccgtgc tagctttaaa
     1861 ggtattgccg gtttagttcg ccgccgtaat gtgtacggta caggtacggt agatgcaaaa
     1921 cgtttagagc aattactgga cgtagcagtt aaagtggcgg caggtacagc accgattgaa
     1981 taccagccga gtcaatataa atggatttta ttaaatcctg aattggcggc gattgaaatc
     2041 ggcacacaac ttgccaaagc ccgtgttgct gatatgctca atacggctat tgccggcacg
     2101 gttgctgcaa ttagtggcaa tacagcaatg acacttgatg aaaagaccgc cgctccgaca
     2161 ttccgcattt taaacaaagg tgcagcgaaa atgggtgatc gtgccggttc gcttaaagcg
     2221 tgggtgttgc actccacaac cttacacggc ttatacgata atgccttaac caatgcggaa
     2281 aacttattca agtacgaaaa cgtgaacgta attcgtgacc cgtttgggcg tgtatttgtc
     2341 gtcacagaca gcccggcttt aatggcttca gacggtacat ttaacacctt aggcttggtt
     2401 gaaaatgcgg tggtagtttc cggcaacaat gactttgaca gcgtgttagt gccgaaaacc
     2461 ggcggagaaa acctttcagc agtttaccaa gctgaatgga cctataactt aggaatccta
     2521 ggttataaat gggatatgac cgctggtggt aaatcaccga acgataccgc cctaggcaca
     2581 ccgacgaact gggataaatc ggttacctcc aacaaagaca ccgccggtgt attggtaaaa
     2641 acgaagtaat ttttaaccgc ttgtagataa ggtttacaag cggttgtttt tttatctgga
     2701 gtttgcaaat gaaacgaata ctttatttta caacggattt ttcggcaaaa aacattgcct
     2761 ttgccaaagc tcacggtgta ttaatgcgga atctgaacgc ttaccaccca tcagacacct
     2821 tagaaaatgc agatgcagtg tgcggtaacg tacctcaagc ttatcaacat ttaccgttgt
     2881 ttgaattgcc tgagtctaac caaaaatcag gtcaagattt gaaaacctta aaaaccgaag
     2941 aaattaagga aaaactgact gaattaggta ttgcttttga ggcgaatgcc aaaaaagaaa
     3001 cattactaac cttactcact caggcggtgg gacgtgacgg aacaaacgat gctaaatagt
     3061 tatatgacgg ttgagcaagc caacgcctat cacgacatca gaatgagccg tgaggcgtgg
     3121 aatgcgttaa gcgatcaaga aaaacagcaa cgattggtgt cagcgtctga ttttctggat
     3181 ttcaattatc gtttttcagg cgaaaaagca gatcttactc aagagcgtga atttccacgc
     3241 aagggttttg aagctatcgg tattcctaaa gccgtgcaat ttgcggtatg tgagttggca
     3301 ttgcaagata acttgaccgc aaatcaagaa gctcaaatga gtagcgtaag agttggcccg
     3361 gtttcagtta attatgataa cagttcaccg caaaacagcg tgaatcggtt tgagtatgtg
     3421 aggcagttgc tttccacttg gttagatcga tcttctttcg gcacggttaa gttggagcgt
     3481 agttgatggc ggatttttac ggcaatttag ggaaaatctc ccattctttg attaagcaat
     3541 tcgggcagcc ttgtagtgta acaattaacg aacaaggcag atacaaccct gcaaccggtg
     3601 agcggaaaaa aggtagctca acggttcacg caggctattg tctgtttgat aatttggcgt
     3661 ttgatttccg caatcagcaa tctgtcagcg ttcaacaagg cgatgtgatg atttatctca
     3721 ctgccgattg caaaccggta ttaaatgctg tgattgaagc aaacggcgaa aaatggtcgg
     3781 tcatcaacgt ccagcctata cgcccagcag ctaccacgat gatttatcaa gtacaaggga
     3841 gaaaagccga tgggtagttt tcacgctcaa atcgcccgtt ttgtggaaca aacgcaacaa
     3901 aaagcggata gggcgttaag ggtaattgcc ctgcaaacct taagtggggt gcagaagaaa
     3961 tcgcctgtcg ataccggaca acttcggcgt agctggacgg tggcattaaa tggtttcccg
     4021 agcagttata acggcagtca aaccgtgctg aatagtgcta aattcggcga tatgattgtg
     4081 attgctacca ataaacctta tgcaccaatg ctcgaatatg ggctttaccc gaacccacca
     4141 aaaaaaccga ccggcaagac caaaaacggt tattcaatac aagcacctaa tggaatggtg
     4201 cgaattaccg tgcaggaaat gcaagccttt atccgcaata atccacaatt aggagtaagc
     4261 tgatgcgacc gattatccgc tcggtattag agcaacatct tgccgaactc aatccgttta
     4321 atacggtttg ggagggcatc atcgacagcc cgaaactgcc gtatcaaacc gtatcaatga
     4381 cgacttcaac caacgaaacc gccacgattg gtaatacgcc gaaagccaaa cagagcggtt
     4441 ttttgcaggt cacgctttac tatccgctag atcaggggac aaaggcgatt gaggaacgag
     4501 ccgatgagat cttccgccac tttttcgggc aggtctggat tgaggaaaat gtgcaaattc
     4561 tggtgcaaca gccaccgctt gtagcctcac tttatcgact ggataacagc attgccttac
     4621 ccatcaccat ctattttacc gcttatgaac tataggagaa cccaatgtct attctaggca
     4681 attcacaagg cacaaaacgt acggtcgttt ttgccaaaga gagttctttc ggcacgaaag
     4741 ctgctccgac aggcgcaaaa attatgcctc gtgttgaaac ctcactcaat accaattttg
     4801 aaagttatca atcggaagag atcagagatg atttacaacg ctccgcctct gtgatcggct
     4861 ttgaaaaggt cgaaggcgat attaaaggcg aactagccgc cggacaatgg gcggcatttt
     4921 ttgcggctgc attacgtggt tcatttaccg ctcaggcgaa aaagccgatt attaccaaaa
     4981 ccacagacgg caccggcgag aaaaacggta aaattttgat tgtgccgaaa accagccaca
     5041 cctccgacag tttcacgctg gaagatgtct tcaaagacat caacgtcagc cgccagtatt
     5101 tgggatgtcg tgtatcgaaa ctcagccttg atgtgcaacc aaacggtatt gcctcgatta
     5161 cagtctcatt tttagggcaa cgaggcgaag aaagtacaac agcctatttt accaatccca
     5221 ccgatttaac ccaatccggc aagctggcag gggtaaaagg ctcattgcag gtcaataaac
     5281 gcccagtcgg gttagtgacg gcctttaaac tggacattga tttaggggca agcagtgagc
     5341 cggtattagg ggcaaaatac gcccctgatg tctttatcgg tacagtggca gtgagcggat
     5401 cgtttacgat gtacttccaa gacaagacga tgattgatgc ggtgcgtaac ggcacagatt
     5461 tatcccttgc tttgcggatg gatgccgaag agcgtaccaa taccgactat ttaaccatta
     5521 tcttaccggg tgtcaaagtg acctcaaacg agttggacga tggggcgaaa aacattatgc
     5581 agacactcaa ttttgacgca ttccctgcgg tatgggattc agcaagccaa atcgacaacg
     5641 tgctaaaagt cgccaccaca atgattgtgc aagacagcct cgcttaatcg aaattttgca
     5701 aaaaaataac cgtatctaac cgcttgccct caaacaagcg gtctttttta tccccaattt
     5761 acaaaaggaa acccaaatga atttaaacgc attaagtaaa gaaaaacagt tagccgaaca
     5821 ccgttttgag ctggttcacc ctgttagtgg cgaaccgctc ggcgtgttta tgagcgtcgt
     5881 gagtgctaag agtgataagg ctcaacgctt tgccgaaaaa caattacgcc aagaacagat
     5941 gagagagctg gaaaatgcca aatcccgcaa gccgaaattc aaaggcttag gcgatatgat
     6001 tgccgaaagt cgtgcaatgg cgattgaccg cttaaccggc tgggagaatg tcgaatggaa
     6061 cgaaaaaccg ttggagtttt ccgaccagaa tgcggagcta cttttaaccg aatgtgattg
     6121 gattgtggag caaatctttg agcattcgaa cgatctggga aagttcttga agagctaatt
     6181 gatgatttac aggtgtatgc agagaaaacg tttaagttgg ataaaaaact taaaggcagc
     6241 gaaagtacct tgcgagagca tttgcaggca atcgaaatgc aaactggcat tacaccagaa
     6301 gaattgcata accctgaacc cagtccagcg gtggcctatc tgctgggctg gttcttcgag
     6361 ttggcaatga gccgacaatc gggtatgagc ttaaacccaa ttagctacac tgaaattgat
     6421 gcgtggagca ggctttttgc tcgtcatctc gaacaatggg agatcagcgt aattaagcag
     6481 cttgatatga tttacttgaa cgtacaacaa acggagtagt atggatactt tcaactttca
     6541 gcccaattgg gggcttaacg tacagagaaa gccagaagtg atgaaaatcg cctttggtga
     6601 cggttacgaa caagtcgccc ctaaagggtt aaaccataat ctccgcactt attccgctgt
     6661 tttcaccggc acagaagcac ggattaaaca gattgaggca ttttttgaac gacaaggcgg
     6721 ctataaggct tttctttgga caccttacgg cgggaaagag gggaaattcc gctgtgaaga
     6781 gtgggaggta agcataagca gcgggaaatg gacgctgtcg gcgacgataa gagaagtgtt
     6841 gtagtttttt ctgattttgt gaactggttc acactctgtg ggttggatat aggttagaat
     6901 ttaagtctaa tttgcttaac ggagtctttt atgaaaaaaa tttttgtttc agccttattg
     6961 ctcacttgct cgttatctgc acttggaaat tcctcgtatg agttgattat tgactcaatg
     7021 caagatcttg aaaagcataa acccgaaatt ctatctatac cagaagcgga tagaaatact
     7081 tttattcgtt atatgatgcg aaaagaactg cggataaagt taaatcaagg cagattagcg
     7141 gatggtctaa ctgtgagaga agctattcaa aatcaacagg catttgaaaa agatagaaat
     7201 agcaaaatgc agcaggagaa gcaaaaaaaa gaaacagaga aagcagaaaa agaaaaagct
     7261 gttacggatt ttaaccaaaa atttgagcta tccattacag gggcgagaaa ggcaaaaaat
     7321 gcactaaatc aagatatttt gcttgtttca ttaagtctaa aaaataactc agataagcca
     7381 attatcgctg tacaaccgga tattcaattt gtactgggtg aagataaaca aactatttca
     7441 tccatgcgtc caaaaaaatt tgaaaacggc attccgccta atggctcaga gatttttgag
     7501 tttgggtttg atttagatga tatgtttgct ttgaaagcag aaaaaaatat agagaaattg
     7561 agggcagtct tccaaacagc agaagttctt tttgctgatg gaacaactga aaaacttcaa
     7621 tagtaattgg taaatcttca ttggccgctt gtagaaatac aggcggtttt tttattggag
     7681 caagctatgg cagatttagc aacactggcg attaaaatcg aaacagaagg cgcaagccga
     7741 gcaagtgcag atctaagtaa ggtcgagcaa tctgcaaaga acacagagaa agccgcagat
     7801 aatctgacgc gcgcaatggg aaatttgaag cggattttag cgattgcagg gctggctggt
     7861 gggttatccg cttacctaga tatggctgaa agaatgcagt cgttaaacgc acagcttaaa
     7921 tttgttacta actctcaata cgaattcaat agcgcacaaa aagagctttt tgcgattgcg
     7981 cagaatacgc gcgctagttt ggaaagcacc acgcaacttt atattaaatc ttctcaggca
     8041 ctgaaagatt acggctacgc gcaaaaggat attctcacat ttaccgaaac cattaacaaa
     8101 gcaatggcag ttggcggggt taagccacaa gagcaagcta gtgctttact ccaattatca
     8161 caggcattag gttcagggaa actgcaagga gatgaattcc gttcaatttc agaagctgca
     8221 ccgattattt tagatacgct tgccgagtat atgggtaaaa gtcgcgatga agtaagaaaa
     8281 ctcgcatccg agggtaagct caccgcagac ttactgttca atgcgtttaa cggttcaagc
     8341 gctaaaatta ccgaaaaatt taatcaaatg ccgctaacat ttggcggtgc aatgcagcag
     8401 ttgcagaatg ccacattgaa atttatcggt gacctagata gtgcaaatgg cttgagtgga
     8461 atgttagctc aagccattag ttttttagca cagaattttg atgtgttagg caaggctttg
     8521 atttatgcaa ccggtgctta tattgcgttt aatgcagttg cctttgctca gaattttgca
     8581 aaagcaaccg gtggtgtcgg tttattaagt agtgcatttg gttatttgac tacgatgatc
     8641 cgaggtgcaa ctgttgcaat gatggctaat cctatcgagc ttttaacaac tgcaattatt
     8701 ggggctgctt ttgtatttga tgcttttatc agtgatatag aggtaggtgc agcaaccttt
     8761 gatgcgactt ggggagatgt tgcgacagct gtgtgggatg attttaaaga tttaacttca
     8821 gatgcggcag attggttagt cactcaatgg gataaagcaa ccgccagtgc agcgagttcg
     8881 ttcagttcct taggtgttga tattggctca gtatgggaga cggtgcaatc cattacgaaa
     8941 acattgatca atagtattat tggtctgttt gttggagctt atcaagccat cgtgcttgct
     9001 tggaataatt ttcctgccac aatggaaaac ttgggtgtga tggcggtaaa tgctcttgtc
     9061 gatttggttc aaaaaggcat caatagcatt attcagctta tcaaattacc gattgagctt
     9121 ttaaacagag cctcatctac ttttggtgga ggaaatctga ttgatacatc aggttggaag
     9181 gcaagcttag atgattttaa actaaaggca agccgcgagg cagaagttgt tggtcaatca
     9241 attagccaag catttgcgga tagtatcacc acagattaca taggcaatgc agttaattca
     9301 gtgagtgact atattgatgg agcggcagtt cgtgggcgct taaaacgtca attagatgaa
     9361 accgcctctg acgatcaagg tacgcagtca ccagctaaaa aaacattagc tggaaataat
     9421 aactctgcta gtgactggaa aaactacctc aatgaacttg aacgagccaa tgccgatgga
     9481 ttagcccgta ttgcacttga gcaagatcgc tcaatgcgtg aaatgttgga aaaggcgaag
     9541 aaagccggtg caagtcacgc tgaaattgaa aaagccaaaa tgctgattac agaacgttat
     9601 gctaaagagc gaatggaaat tgccgagaag tatgtgccga gtctgaagtt tgaggaagaa
     9661 ctcaaagatc agattaagga aattgatgaa ctgcaaaagt taaacctgat taccaccgag
     9721 caagcaggtc ttgcgaaaga ggcgttagca ggcaaatatc agccggtagt tgaaatccaa
     9781 cagcaataca tcaatcaact gagagagatt gaagcattaa aacaagccgg tgtattaagc
     9841 aaacctcaag cagaagtagc ggccgaaaaa gccaaatggg acgccggcac acaaaaagcc
     9901 aaaatcgcag gtaaaaacgc cgttagccct tatgaaaaat ggaaatcgga atttgaccca
     9961 atgcaggcaa ttcaaaatga gcaagcctca aagttggcag aagtgcaatc gatgtacgat
    10021 cagcatttga tccaatatga ggattttatc aacgctaaag cacaaattga tgctaaggcg
    10081 actgctgata ctcatcaact gtttatgaac agtattagtg ggtttggttc ggcgatagat
    10141 acgatgttag gcgtgatgaa aaacgcaggg caagagcaat caggtattta ccgcacgatg
    10201 tttgcgatgt cgaaagcgtt tgctattgcg gaatcaattc tgaacttgaa tcgtgcaatt
    10261 actcaagcaa tgagcgatca aacagctatt acaccggctc aaaaatttgc aaatatggcg
    10321 gctattgcta gtgccggggc gagcttgatt tctaatatcc agtcagtctc attaggcggt
    10381 atggctcaca gcggtattga caatgtgccg aaagaaggca cttggttgct ggataaaggt
    10441 gagcgtgtag tggatagtcg tactaaccaa gacttaaaaa cctttttatc caatgctaat
    10501 cgtggcggtg caagtggtca aagatcggcg ataaatgtca aaattattaa taacgggcaa
    10561 cccgtagagg cgaaagtcag cagtgaagag actgtagatg gcaatatcca aatgacggtg
    10621 gaactgctga agaaaatgga tcaaatcgcc gatcaacgct accgtagaaa tcagttaaat
    10681 gatttaagaa caggtggaac attatctaaa taggtgaagt atgccaatta gtatttctaa
    10741 ccaaatgaaa ctagatcttg ccaaacttga gcaaaatgca atgctggact tgtacgaggt
    10801 tgatttgcgc aatctcaaag ataaaagtgg taatgctggc agagtttacc gcttttattc
    10861 tgggctaaat gaactcaaaa caagtatcgt ctggcaaggg cggacttacg atccgtatcc
    10921 tattgaggca agtggatttg aacgaaacag tagcggcccg agcaatcgcc cgactctcac
    10981 gctatcaaat ctgtttggtt taattaccag tattgcgaat cagtttgacg aatgtatcgg
    11041 tgcgattgtc cgccggcatc aggtttatgc tcaatattta gatgccgtga attttgccgg
    11101 gggtaatgcg aaagctgatc caaatcagga aataatcagt cattttgtga ttgagcagct
    11161 taccagcctc acccgagaaa cggcgacctt tacgctggca ttaccgattg aaaccgataa
    11221 tgccaagatc cccagcagaa ttattatggc ggacacctgc acttggattt accgctcggc
    11281 ggaatgtggc tacaccggca agca
//
LOCUS       DANLXS010000073        11247 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.073, whole genome shotgun sequence.
ACCESSION   DANLXS010000073 DANLXS010000000
VERSION     DANLXS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.073"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            114..1910
                     /locus_tag="PXD71_001397"
     CDS             114..1910
                     /locus_tag="PXD71_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate-specific subunit IIBC"
                     /protein_id="HDL6291512.1"
                     /translation="MDFLTEILLFLKDQVLNKAPFLLGIVACLGYILLKKDTSTIIKG
                     TIKTIVGFMIVQAGSGFLVTNFKPVIDGLAKYHQLTGAVIDPYTSMQSTIQTMGENYA
                     WVGYAVIGALFLNILLVVFRRLTGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETII
                     YTSVLMALYWGISSNIMYKPTQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKDDTVD
                     KMKLPNWLNIFHDSISATALVMTIFFGIILLSFGLDNLKELAGKTNQFMYILEMGLKF
                     AVAIQIIVTGVRMFVAELSEAFKGIAERLIPNSVLAIDCAAIYAFSPNAMVFGFMWGA
                     IGQFVAVGGLLAVGSPVLIIPGFIPMFFSNATIGVFANQFGGWKAVMKICFVMGIIEV
                     LGSAWVIQMLASQGTTFNGWMGMADWALFFPPILQGIISIPGFFFVIVAAAMVYMFFA
                     SKQLRAEEAEAAARGITLEQLDGYGLDEVKAEPKAEAVVQQAVENAENSANPIRILAV
                     CGSGQGSSMMMKMKIKGYLDKRGIPNIMDSCAVTDYKGKLSEVDIIVSSKHLANEIEV
                     PEGKAVLGVQNMLNPNSFGDELVELINQHNAK"
     gene            1990..3051
                     /locus_tag="PXD71_001398"
     CDS             1990..3051
                     /locus_tag="PXD71_001398"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL6291513.1"
                     /translation="MKKILSTLFMLSAVSVAVAKEVNIKLLGTSDVHGRVVPWSYGAD
                     VEDKSGSFAQIATYVKEVRANHKNVLLFDIGDAIQDNQVEEFAKTLDKAKNNPVPKVL
                     NAMNYDIFILGNHEFNFGMPNLDAILKDINAKILTANFYHKDGKRYVEPTTIIERDGV
                     KLGIIGLTTPMSAIFEKDTGHLDAMKFTSPIEEAKAQIADLKAKGVDAIIVLAHMGIE
                     NENNIPDTGVADLVNAVDGIDVIIAGHMHKDVPSETIKNTLITEPHRYGTVVSEVDLA
                     FDVNDKGEVKLLSKTAKTVPVKKLASDPEIEKIYQPYHNELRALNNVKIGETAQDLIP
                     QGKITVYRLLSRKTPECPH"
     gene            3039..3503
                     /locus_tag="PXD71_001399"
     CDS             3039..3503
                     /locus_tag="PXD71_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDL6291514.1"
                     /translation="MSSLIHDVQQHYSGADVVSFAFDYETVRLDKGDIKKKDIIYNYR
                     YAGGDVSMYELTGKQLKQYMEWSADYFDTIQAGDKDYRYNAVRGKSKYVTFDLFGGVS
                     YNIDLRNPSGSKIVDLKLANGSLITDDGKYKVGMNSYRFGQLTKKGGIWEGQ"
     gene            3534..3650
                     /locus_tag="PXD71_001400"
     CDS             3534..3650
                     /locus_tag="PXD71_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser/Thr protein phosphatase"
                     /protein_id="HDL6291515.1"
                     /translation="MGAEKGTIQNMLIDYITNVKGGKIEGKSHNRWAIIGLN"
     gene            3666..4133
                     /locus_tag="PXD71_001401"
     CDS             3666..4133
                     /locus_tag="PXD71_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL6291516.1"
                     /translation="MNLKQSLIENNSILLNQSAANWEDAIKIGTDLLVKAGTIEPRYY
                     DNIVSKIKEMGPYIILAPGLAMPHARPEEGVIKTSFALVTFDTPIYFEGEDEPVHVML
                     TLAGSDSDQHMQGLVEITQVLDDPESDTGVDLDKIRRCKTAEEVYAVIDQALA"
     gene            4188..4868
                     /locus_tag="PXD71_001402"
     CDS             4188..4868
                     /locus_tag="PXD71_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL6291517.1"
                     /translation="MSKPLLQIALDSLSLEKAVADAKQAENQVEIIECGTILAFAEGM
                     KAVSTLRVLHPNHIIVCDLKTTDGGAILAKMAFEAGADWLTVSAAAHPATKAACKKVA
                     DEFNAAHPELKVKKEIQIEIYGNWTVEKDAKEWVELGVTQAIYHRSRDAELAGKGWTT
                     EDVELMKQLSDLGIELSITGGIVPEDIHLFKEIKNAKAFIAGRALVGEKGKQTAEAIR
                     VEIDKYWN"
     gene            complement(4936..6078)
                     /locus_tag="PXD71_001403"
     CDS             complement(4936..6078)
                     /locus_tag="PXD71_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL6291518.1"
                     /translation="MKIVIAPDSFKESLTALEVAQAIQQGFARVFPEAEYQLVPMADG
                     GEGSVQSLVDATHGSLQKISVIAPLANEVEGFFGLSGDQKTAFIEMAAASGLHLVPME
                     QRNPLVTTSYGTGQLIKAALDLGVEKIILGIGGSATNDGGVGMLQALGAKFLNAEQRE
                     IGFGGEALSQIQQIDLSGLDSRLKQVEFEVACDVDNPLCGERGASAIFGPQKGATPEM
                     VKMLDHALLHFAETVKSQLGIEIAEKAGAGAAGGMGGGLLLLPNVTLRSGVQIILEAT
                     DLAEKLADADLVVTGEGRMDAQSIAGKTPIGVAKVAKQFGKPVIAIVGSLKEDYPVVY
                     QHGIDAVFPIIRQVSNLEAVLKSGKENLISTAENVARLWKISALQE"
     gene            complement(6088..7467)
                     /locus_tag="PXD71_001404"
     CDS             complement(6088..7467)
                     /locus_tag="PXD71_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL6291519.1"
                     /translation="MSGIALILIFLVAIIIMIYMIAKWKVHPFLALMSISIALALVAG
                     LPLAKIPTIVGEGFSNTFKSIGIVIIFGAIIGTILEKTGAALKLADMVVKVVGQKRPE
                     LAMLIMGWVVGIPVFCDSGFVVLNPIREAIRKKILANPVGMAVALSAGLYASHVFIPP
                     TPGPIAAAGAVGLSHNLLLVIGMGFVVSIPVLIASYFFAKYVGKQVSVMEEAQADQVI
                     SQSYEDLIKQYGKLPSGFLSFAPIFMPIILMAIGSIAKIAGVSGDFGVFLQFVGNPII
                     ALAIGVIFAIFLLVSSGKMAEFDSLTNDTLKLVGPILFITAAGGVLGNVITQAGFVDY
                     IKQNAHIISTAGIFFPFLISAILKTAQGSSTVAIITTASIMGMFNAPDSLMNALGLTS
                     EMAAALTVMAIAAGSMCVSHANDSYYWVVTNFTKLTPQQGYRTQTTLTFIMGVVGMLT
                     VYLLSLILL"
     gene            complement(7589..8683)
                     /locus_tag="PXD71_001405"
     CDS             complement(7589..8683)
                     /locus_tag="PXD71_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6291520.1"
                     /translation="MKLNQAIADRIVKRTMQIIPNSVNVMDSEGVIIASGDVGRIGQR
                     HTGAVIALRNDQAVEIDENLAKQWCDEVKAGINLPLHYLGSTIGVVGISGQPQQVRQY
                     AQLVKMAAELIMQQAFELEQERWQRRYREEFVRALLKGSLSTAEIQQQAVFFGQDFAK
                     PLSVMVIKIHGSTAEKLQRLIDFFEHYFPSLLTAVIGLDKIALLNHLSNEKSLIRNAL
                     NKLNDPDISFKTVVGLAVENLSQTHISYQTACHTLNYAEQIQSRKQIIHFEDFKLPAL
                     LHSFSHSWEAKTLLSPFQALLVSDKKQVLLKSLRYYFFANCDLDHAAKKLFIHPNTLR
                     YRLEKIEQITGLSFNNIEQKFVLYLGAVLS"
     gene            complement(8787..9239)
                     /locus_tag="PXD71_001406"
     CDS             complement(8787..9239)
                     /locus_tag="PXD71_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HDL6291521.1"
                     /translation="MNLKAVAVSALAMSSVAMADIADVKPVVFESKFDFQTTVTTLTN
                     TLKEKGMTIFAEIDHQAAAKQAGLEMQPATVLVYGTPKAGTPLMVKDPMLALQLPLKV
                     LITEPNKGKVEVMLNRADQVVAHANTDYADVENNLAKAEKLIKATVAK"
     gene            complement(9260..10012)
                     /locus_tag="PXD71_001407"
     CDS             complement(9260..10012)
                     /locus_tag="PXD71_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL6291522.1"
                     /translation="MHFEKRSHWSGELGREMSFNVYGHAGKPVIVFPSSGGNENEYGN
                     FGMIEACRSFIERGLIKFYTPDSYDAESWLSKHKSGHDMALAHNAYDRYIIHELVPLI
                     RHESHWNGTMIATGCSMGAFHTVNFALRHPDLFDIAIALSGVYDARFFSGEFYGDKAV
                     YFNSPIDYVWGQNDDWFLDRYRQNHYVIAVGQGAWEEQHIEDTRRLQEAFNAKGIDAW
                     FDYWGFDIDHDWPTWRKQMPYFLGKLEEQGII"
     gene            complement(10023..11207)
                     /locus_tag="PXD71_001408"
     CDS             complement(10023..11207)
                     /locus_tag="PXD71_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL6291523.1"
                     /translation="MSRPLNFVMISPHFPTNFETFAIRLREAGFNTLGIADAPYESLS
                     ENLRHSLTEYYRVDNMEDYEQVYRAVAYFAHKYGRIDRIESHNEYWLELDAKLRTDFN
                     VFGYKSDDMLSIKTKAQMKEVFRKTGLKVAQGRVFKDDEDARKLAKQLKFPVIIKPNS
                     GVGASDTYKIKTAEELEAFFGYKTPTVEYIMEEFIDGDIITFDGLTDKEGNIVFYSSL
                     EYSEAVLDTVSKDGDMFYYVPCEISPKLVEMGKQCVKAFNVKERFFHFEFFRVKGSKK
                     EELLPLEINCRPPGGLTIDMWNYANDFDVFREFANIVKENKFYADIQHPWNVVYISRK
                     ANQNYVNSIEAVCEKFAGNIISVQSVPGVFAKVMGEQGILARTETMEQMREIAQFAQA
                     KQ"
BASE COUNT         3235 a         2587 c         2352 g         3073 t
ORIGIN      
        1 tcacaaataa ttaaaaataa tcttttttaa tctttttcta gcgtttatca ttaattccga
       61 caacttctct ggagtttttt agttctatta atctctcaac aaagaggccc cctatggact
      121 ttttaaccga aattctcctt ttcttaaagg atcaagtgct gaataaagca cccttcttac
      181 taggtatcgt agcttgctta ggttacattc ttcttaaaaa agacacttct acgattatta
      241 aaggcacaat taaaacaatt gtcggtttta tgattgtaca agccggttca ggtttcttag
      301 taaccaactt taagccggtt attgatggct tagccaaata tcaccaactt accggtgcag
      361 taattgaccc ttacaccagt atgcaatcga ccattcaaac catgggcgag aactacgctt
      421 gggttggtta tgccgtaatt ggggcgctgt tcctcaatat tctattagtg gtgttccgcc
      481 gtttaaccgg tatccgtacc attatgctca ccggtcacat tatgttccaa caagcaggct
      541 tggttgcggt gttctatatg atcatcggag cgtcaatgtg ggagaccatt atctacacct
      601 cagtgttgat ggctctttat tggggtattt cgtctaatat tatgtataaa ccaacccaag
      661 cggtaacagg cggtgcaggc ttctcaattg gtcaccaaca acaaattgct tcttggattg
      721 cagtcaaact cgcaccaaaa cttggcgata aagacgatac tgtagataaa atgaaattgc
      781 ctaactggct caatattttc cacgacagta tttctgcaac cgcattagta atgaccatct
      841 tctttggaat catcttactt tcattcggtt tagataacct caaagaactt gctggcaaaa
      901 ccaaccagtt tatgtacatt cttgaaatgg gcttgaaatt tgcggtggca attcaaatca
      961 ttgtaaccgg cgtacgtatg tttgtagctg agctttcaga agccttcaaa ggtattgctg
     1021 aacgcttaat tccaaactca gtattagcga ttgactgtgc cgctatctac gctttctcgc
     1081 caaatgcgat ggtattcggc tttatgtggg gggctatcgg tcaatttgtg gcagtaggcg
     1141 gcttgcttgc agtaggctca ccggtgctta ttattccggg ctttatcccg atgttcttct
     1201 ctaacgcaac cattggtgtg tttgcaaacc aatttggtgg ttggaaagca gtaatgaaaa
     1261 tctgttttgt gatgggtatt attgaagtgc ttggctcagc ttgggttatt caaatgcttg
     1321 cttcacaagg cacaaccttt aacggttgga tgggtatggc tgactgggca ttgttcttcc
     1381 caccaatcct acaaggtatt atcagcattc cgggcttctt ctttgtaatt gtggcagcag
     1441 caatggttta tatgttcttc gcatctaaac aacttcgtgc cgaagaagca gaagcggcag
     1501 ctcgtggcat tacacttgaa caattagacg gctacggctt agatgaagta aaagcagaac
     1561 caaaagcgga ggcagtagta caacaagcgg tcgaaaatgc cgaaaattct gcaaacccaa
     1621 tccgcattct agcggtgtgt ggctctggtc aaggctcatc aatgatgatg aaaatgaaga
     1681 tcaaaggcta tctcgacaaa cgtggtattc cgaatattat ggattcctgt gcggtaactg
     1741 attacaaagg caaattaagc gaggttgata ttattgtctc cagcaaacat ttagccaacg
     1801 aaatcgaagt accggaaggc aaagcggtat taggtgtaca aaatatgcta aatccaaact
     1861 catttggtga tgaattagtt gagctaatca atcagcataa tgcaaaataa gtaagtttag
     1921 gggtgaataa tcacccctat ctatgctaaa gattgtaaaa actcacttca tcaaacgagg
     1981 aattatccaa tgaaaaaaat cttatccacc ctgtttatgc tctccgcggt tagcgttgcc
     2041 gttgccaaag aggtaaacat taaactactt ggtacctcag acgttcacgg acgtgttgtg
     2101 ccgtggagct acggggcaga cgtggaagac aaatccggct cattcgccca aattgcaacc
     2161 tatgtcaaag aggtgcgtgc caaccacaaa aatgtgttgc tgtttgatat tggcgatgcg
     2221 attcaagaca accaagtcga agagttcgct aaaaccttag acaaagcgaa aaacaatccc
     2281 gtgccgaaag tgctgaatgc aatgaattac gacatcttca ttctcggcaa ccacgaattt
     2341 aacttcggaa tgccgaattt agatgcgatt ttaaaagaca tcaacgccaa aattctcact
     2401 gccaacttct atcacaaaga cggcaaacgc tacgttgagc caactaccat cattgaacgt
     2461 gatggcgtaa aacttgggat tatcggctta accaccccaa tgtcggctat ttttgaaaaa
     2521 gacaccggtc atttagacgc aatgaagttc acttcgccga ttgaagaggc gaaagcccaa
     2581 attgccgatc tcaaagccaa aggcgtggac gccattattg tgctggcaca tatggggatt
     2641 gagaacgaaa acaacattcc cgacaccggc gtggcggatt tagtgaatgc agtggacggt
     2701 attgatgtaa ttatcgccgg ccatatgcat aaagatgtgc cgagtgaaac tatcaaaaat
     2761 accctgatta ccgaaccgca ccgctacggc acagtggttt ccgaagttga tcttgccttt
     2821 gatgtgaatg acaaaggcga agtgaaacta ctttctaaaa ccgccaaaac cgtaccggtg
     2881 aagaaactcg caagcgatcc ggaaatcgaa aaaatttacc aaccgtatca caatgaactg
     2941 cgtgccttaa acaacgtcaa aatcggtgaa actgcacaag atttaatccc acaaggcaaa
     3001 atcacggtgt atcgattgct ttctcgcaag acaccggaat gtcctcattg atccacgatg
     3061 tgcaacaaca ttacagcggt gcagacgtag tgagttttgc atttgactac gaaaccgtgc
     3121 gtttagacaa aggcgacatc aagaaaaaag acatcatcta caactaccgc tatgccggtg
     3181 gcgatgtgag catgtatgag ctaaccggca aacagcttaa acagtatatg gaatggtcag
     3241 cggattactt cgacaccatt caagccggtg acaaagacta ccgctacaac gctgtgcggg
     3301 gtaaatctaa atacgttacc ttcgacctat tcggcggcgt aagctacaac atcgacctac
     3361 gcaatccaag cggtagcaaa attgtcgatc taaaactggc aaacggcagt ctaatcaccg
     3421 atgacggcaa atacaaagtg ggaatgaact cctaccgctt tggtcagctc accaaaaaag
     3481 gcggcatctg ggaaggacag taaatccccg tgatttggga atccaaagtc gccatggggg
     3541 ctgaaaaagg cacaatccaa aatatgctga ttgattacat caccaacgtc aaaggcggaa
     3601 aaatcgaagg caaatcgcat aaccgctggg cgattatcgg cttaaattaa cgaaagggaa
     3661 acactatgaa cttaaaacaa tctcttattg aaaataactc tattttgctc aatcaatcag
     3721 cggcgaattg ggaagacgca atcaaaatcg gcactgattt attagtcaaa gccggtacga
     3781 ttgaaccacg ctattacgat aatattgtaa gcaaaatcaa agagatgggg ccttatatta
     3841 ttcttgcacc gggcttggca atgcctcacg cccgtccgga agaaggtgtg attaaaacct
     3901 ctttcgcttt agtgactttt gacaccccga tttattttga gggcgaagat gagccggttc
     3961 acgttatgct aacccttgca gggagtgact ctgatcagca tatgcaaggc ttggtagaaa
     4021 tcacccaagt gctagatgac ccagagagcg acacaggtgt tgatttagat aaaatccgcc
     4081 gctgtaaaac ggctgaagaa gtgtatgcag taatcgatca ggcattagcc taatattcta
     4141 caagcggttc aaaaacatca atattttgca aatagaggaa tacaattatg tccaaaccat
     4201 tattacaaat tgcccttgat tctctttcac tggaaaaagc ggtggcggat gcaaaacaag
     4261 cagaaaacca agtcgaaatt attgaatgcg gtactattct tgcttttgct gagggtatga
     4321 aagccgtttc caccttgcgt gtattgcacc caaatcacat tattgtgtgc gacttaaaaa
     4381 ccaccgatgg cggtgcaatt ttagctaaaa tggcatttga agccggtgcc gactggctca
     4441 cggtttcggc tgcggctcac cctgcaacca aagcggcttg caaaaaagtg gcggacgaat
     4501 ttaacgctgc tcacccggag ctaaaagtga aaaaagagat tcaaattgaa atctacggca
     4561 actggacggt ggaaaaagat gccaaagagt gggtagaatt aggggtaacc caagcgattt
     4621 accaccgctc ccgtgatgcg gaactagcag gtaaaggttg gacaacggaa gatgttgagt
     4681 taatgaaaca gctttctgac ttaggcattg aattatctat caccggcggt atcgtaccgg
     4741 aagatattca cctgttcaaa gagatcaaaa acgccaaagc ctttattgca ggccgtgctt
     4801 tagtcggtga aaaaggcaaa caaactgccg aagccattcg tgtggagatt gataaatact
     4861 ggaactaatc cggcattgaa acccaataaa aatggcgttc tctcaaccgg aacgccattt
     4921 tttatctcat tttttctatt cttgcaaggc cgatattttc cacaatctcg ctacattttc
     4981 ggcagttgaa atcaggttct ctttgcctga ttttaacacc gcttctaaat tgctgacctg
     5041 tcgaataatc ggaaataccg catcaatgcc gtgttggtac accacagggt aatcttcttt
     5101 caatgagcca actatcgcaa tcaccggctt gccaaactgc ttagcgactt tcgccacccc
     5161 aatcggggtt ttaccggcga tagattgagc gtccattcgc ccttcccctg tcaccaccaa
     5221 atccgcatcg gcaagtttct cagcaagatc ggttgcctca agaataattt gcacgccgga
     5281 acgtaatgtt acgttaggca gtagcagtaa accaccgccc attccaccgg ctgcaccggc
     5341 accggctttc tcggcaatct caatgcctaa ttgggatttt acggtttcgg caaaatgcaa
     5401 cagggcgtgg tcgagcattt taaccatttc cggtgtagct cctttctgtg gcccaaaaat
     5461 tgccgaagcc cctcgctcgc cacacagcgg attatcgaca tcgcaagcaa cctcaaattc
     5521 cacttgcttt aagcgtgaat ccaaaccgct taaatcaatc tgctgaattt ggcttaatgc
     5581 ctcgccacca aagccgattt cccgctgttc ggcatttaga aatttcgctc ctaaggcttg
     5641 tagcattccg acaccgccat cgttagtcgc actgccgcca atgccgagaa tgattttttc
     5701 cacccctaaa tctaaggcgg ctttaattaa ctgccccgtg ccgtagctgg tggtaaccag
     5761 cggatttcgt tgctccattg gcactaaatg caagccactt gccgccgcca tttcaataaa
     5821 ggcggttttt tgatcccccg acaagccaaa aaagccctcc acctcatttg caagcggtgc
     5881 aattacgctg attttttgca aactgccgtg agtggcatca accagcgact gtaccgagcc
     5941 ttcgccaccg tctgccatcg gtacgagctg atactccgcc tcagggaaaa cacgggcaaa
     6001 accctgttga attgcttgag ccacttctaa tgcggtcaaa ctctctttaa atgaatccgg
     6061 tgcaatcaca attttcatcg tcaatcctta gagtaaaatc aatgaaagca gataaaccgt
     6121 gagcataccc accacaccca taatgaaagt tagggtagtt tgcgtacgat agccttgttg
     6181 cggagtcagt ttagtaaagt ttgttaccac ccagtagtag ctgtcattgg cgtgggaaac
     6241 gcacattgag ccagccgcaa tcgccattac ggttaaggct gccgccattt cactggttag
     6301 ccctaaggcg ttcattaatg agtccggtgc gttgaacatt cccataatgg aggcggtggt
     6361 gataattgcc accgttgagc tgccttgtgc ggtttttaaa attgccgaaa tcaggaacgg
     6421 gaagaaaatc cctgctgtac tgataatatg agcgttttgc ttgatgtaat ccacaaagcc
     6481 ggcttgagta atcacgttac ctagcacacc accggcggcg gtaataaaga gaattgggcc
     6541 gactaatttc agggtatcat tggttaagct gtcaaattcc gccattttac cgcttgaaac
     6601 cagtaggaaa atagcgaaga tcaccccaat tgctaacgca ataatcgggt tgccgacaaa
     6661 ttgtaggaat acgccgaaat cgccacttac accggcaatt ttggcaatcg agccgatagc
     6721 cattaagata atcggcataa aaatcggggc aaagcttaaa aacccacttg gtaatttgcc
     6781 gtattgtttg atcagatctt catagctttg gctgattact tgatccgctt gagcttcttc
     6841 catcacgctg acttgtttgc caacatattt ggcaaagaag taactggcaa tcaacacagg
     6901 aatcgaaacc acaaagccca ttccgatcac aagcaggaga ttatggctta aaccaacagc
     6961 accggctgct gcaatcgggc ccggtgtcgg aggaataaag acgtgggagg catataaacc
     7021 ggcacttaac gccaccgcca ttcccaccgg attggcaaga atttttttgc gaatagcttc
     7081 acgaatcggg tttaacacca caaagccgct atcacagaac actgggatac caaccaccca
     7141 gcccataatc aacattgcca gttcagggcg tttttgcccc accactttta ccaccatatc
     7201 ggctaatttg agggctgcac cggttttttc taaaatcgtg ccgataatcg ccccaaaaat
     7261 aatcacgata ccgatacttt tgaaggtatt actaaaacct tcgcccacaa tagttggaat
     7321 tttggcaagc ggtaaacctg ccactaatgc aagggctatc gaaatcgaca ttaaggctaa
     7381 gaacgggtga actttccact tcgcaatcat ataaatcatt atgatgatag cgaccagaaa
     7441 gatcagaata agtgcaatac ctgacataat aagtctcctc attgtagaaa aatgaactta
     7501 ttattcggta tttattgcta aaaagattta gataaatttg caaaaaattt gttaaaaacg
     7561 accgcttgtt tgtatatttt tacaattttt acgacagaac cgctccaaga tacagcacaa
     7621 atttttgctc tatgttattg aaagataaac cagttatctg ctcaatcttc tctaaacggt
     7681 aacgtagcgt atttgggtga ataaacaatt ttttggcagc gtgatccaga tcacaatttg
     7741 cgaaaaaata gtaacgcaga cttttcagta acacctgttt tttatcggaa accagtaaag
     7801 cttggaacgg cgagagcaag gttttcgctt cccaagagtg gctgaaactg tgcaagaggg
     7861 ccggcagttt aaaatcttca aaatgaataa tctgcttacg gctttggatc tgctcggcat
     7921 aattgagggt atggcaggcg gtttggtagg aaatatgggt ttgcgataaa ttttcgaccg
     7981 ccagcccaac caccgttttg aaggaaatat ccggatcgtt aagtttgttt agtgcattgc
     8041 ggattaacga tttttcgttg gataaatgat tcagcaaggc aattttatct aacccaatca
     8101 cggcggtgag taggctcggg aaataatgtt cgaaaaaatc aatcaggcgt tggagttttt
     8161 ccgcagtaga gccgtgaatt ttaatcacca tcaccgacag cggttttgca aaatcttgcc
     8221 cgaaaaagac cgcttgttgc tggatttctg ctgtgcttag gctacctttg agcaaggccc
     8281 gcacaaactc ttcccgatag cggcgttgcc aacgttcctg ttccaactca aacgcttgct
     8341 gcataatcag ctccgccgcc attttcacca attgagcata ttgccgaact tgttgtggct
     8401 gccctgaaat ccccactaca ccaatggttg agcctaaata gtgaagcggt aagttaatgc
     8461 ccgctttcac ttcatcgcac cactgttttg caagattttc atcaatttcg accgcttgat
     8521 cgttgcgtaa cgcaatcacc gctcctgtgt gtcgttgccc gatacgaccg acatcgccgg
     8581 aagcaataat caccccttca ctatccatca cattgacgga gttgggaata atctgcatcg
     8641 tgcgcttgac gatgcggtcg gcaatcgctt ggttgagttt cattatatga ttcctactca
     8701 ttcccaatac tatgaaaaat ccctctttcg agggatttgc aaattatcta cgcttccact
     8761 tcctgagaat agatcatagc gtgaagttat tttgccacag tcgctttaat taatttttcc
     8821 gctttggcaa ggttgttttc tacatcagca taatcggtat tagcgtgagc caccacttga
     8881 tcggcacgat ttaacattac ttcgaccttg cccttgttcg gttcggtaat taacactttt
     8941 aacggtaatt gcaacgctaa catcggatct ttcaccatta atggcgtacc ggctttcggt
     9001 gtgccgtaaa ccaatacggt tgccggttgc atttctaagc cggcttgttt tgccgccgct
     9061 tggtgatcga tttcggcaaa aatcgtcatt cctttttctt ttaaggtgtt ggttaaagtt
     9121 gtgactgtag tttggaaatc aaatttactt tcaaatacca ccggtttgac atcggcaata
     9181 tctgccattg ccacgctgct cattgctaat gcagaaactg cgactgcttt taagttcatt
     9241 tcttttctcc tcaaatagat taaataatcc cttgttcttc caacttgcct aagaaataag
     9301 gcatctgttt acgccaagtc ggccagtcat ggtcaatatc aaagccccag tagtcaaacc
     9361 aagcatcgat cccttttgcg ttaaaagcct cttgtaatcg gcgggtgtct tcaatatgct
     9421 gttcttccca cgcaccttgt ccgactgcaa tcacatagtg attttggcgg tagcggtcta
     9481 agaaccaatc gtcattttga ccccatacat aatcaatcgg cgagttaaaa taaaccgctt
     9541 tatcgccata aaactcacca ctgaaaaagc gagcgtcata aacaccgctt aaggcgatgg
     9601 caatatcaaa cagatccgga tgacgtaagg caaagtttac cgtatggaaa gcccccatac
     9661 tacaaccggt ggcaatcatc gtgccgttcc agtgcgattc atggcggatt aacggcacta
     9721 gctcgtgaat aatataacgg tcgtaagcat tatgagctaa tgccatatcg tgtccggatt
     9781 tatgtttaga taaccaagat tccgcatcgt aagaatcagg cgtgtaaaat ttgattaagc
     9841 cacgttcaat aaaggaacgg caagcctcaa tcattccaaa attaccatac tcgttttcat
     9901 tgccgccgga ggaagggaaa acaataaccg gtttacctgc gtgtccgtaa acgttgaacg
     9961 acatttcacg acctaattct ccactccagt ggcttctttt ttcaaaatgc atacttcacc
    10021 tcttactgtt tagcttgtgc aaattgtgca atttcacgca tttgctccat tgtttcggta
    10081 cgggctaaaa tgccttgttc gcccatcact ttggcaaata cgcccggtac gctttgcacg
    10141 ctgataatat tgccggcaaa tttctcacaa acagcttcaa tcgaattcac ataattttgg
    10201 tttgccttgc gggaaatata aaccacgttc caagggtgct gaatatcggc atagaatttg
    10261 ttttctttca caatattggc aaattcacgg aacacatcga aatcgttggc gtagttccac
    10321 atatcaatag ttaagccacc cggcggacgg cagttgattt cgagtggtaa caactcttct
    10381 tttttgctcc ctttaacacg gaagaactca aaatggaaaa aacgctcttt tacgttaaag
    10441 gctttcacac attgtttgcc catttccact aattttggcg aaatttcaca tgggacatag
    10501 tagaacatat cgccatcttt tgaaacggta tctaataccg cttctgaata ttcaaggctg
    10561 gagtagaaaa cgatattgcc ctctttatcg gttaaaccgt caaaggtaat aatatcgccg
    10621 tcaataaact cttccataat atattcaacc gttggcgttt tataaccgaa gaaagcttct
    10681 aactcttccg cagttttgat tttgtaagta tcgcttgccc caacccctga atttggcttg
    10741 ataatcaccg ggaatttaag ctgttttgct aatttgcggg catcttcatc atctttaaat
    10801 actcgccctt gtgccacttt caagccggtt ttgcggaata cctctttcat ttgggctttg
    10861 gttttaattg agagcatatc atcgctctta taaccaaaca cattgaaatc ggtacgcaat
    10921 ttcgcatcta actccagcca atattcattg tgcgattcaa tgcggtcaat tcgcccgtat
    10981 ttatgggcaa agtacgcgac cgcacgataa acttgttcgt aatcttccat attatctaca
    11041 cgataatatt cagtcaggga atgacgcaga ttttcgctca atgattcata aggggcatct
    11101 gcaattccca atgtattaaa accggcttca cgcaaacgaa tggcaaaggt ttcaaagttc
    11161 gttgggaagt gcggggaaat catcacaaaa ttaagtggtc tagacatagt tcttccttta
    11221 taaagtttgc atattattaa aatgatt
//
LOCUS       DANLXS010000074        11027 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.074, whole genome shotgun sequence.
ACCESSION   DANLXS010000074 DANLXS010000000
VERSION     DANLXS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11027
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.074"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            163..909
                     /locus_tag="PXD71_001409"
     CDS             163..909
                     /locus_tag="PXD71_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL6291524.1"
                     /translation="MQLRHKQIMEYIQQFDEANVQELAEHCGVSIETIRRDLNKLDKD
                     GLLHRTHGGAVSHKKRDIGRSFTTRQRLNSEAKRSIAENVLEYLQPESVIALDASSTS
                     WNVAQRIPNIPCTVVSSSMRIIYSLSHKPHIKTIATGGIYSEKYDAFYGPLSEQLLTR
                     LKIDLAILSCTGIADGAIWESNEINISLKRKLIANSKQVFLLADHSKFERKDLFRIED
                     LSCIDKLFINQPPPDFLKNYCLAHQIEIII"
     gene            1105..2868
                     /gene="fucI"
                     /locus_tag="PXD71_001410"
     CDS             1105..2868
                     /gene="fucI"
                     /locus_tag="PXD71_001410"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDL6291525.1"
                     /translation="MTTLKSTPVKIGIRPTIDGRRLGVRESLEDQTMNMAKAVAQLIQ
                     SEVRHPNGDFVECVIADSTIGGVAEAAACAEKFKRENVGAVITVTPCWCYGSETIDMD
                     PHMPKAIWGFNGTERPGAVYLAAALAGHSQLGLPAFSIYGTEVQEADDQSIPDDVREK
                     LLHFARAGLAVATLRGKSYLSIGSVSMGIAGSIVNQQFFQEYLGMRNEYVDMTEIKRR
                     LDRHIYDAEEFKLAMSWVKTYCKEGIDVNAPENQRSPEEREKLWEDVVKMTIIARDLM
                     VGNPRLAELGYSEEALGHNAIAAGFQGQRQWTDHLPNGDFMEATLNSTYDWNGVRAPY
                     IMATENDSLNGVNMLFGNLLTGQAQIFADVRTYWSEDSVERVTGWRPESGFIHLINSG
                     SAALDGTGQHTDRAGKPTIKPAWEVTEEDGKRCLENTCWCPAVHEYFRGGLSSQFLTK
                     GGMPFTMHRINLIKGIGPVLQIAEGWSIDLPENVHDILNKRANETWPTTWFVPRLTGK
                     GAFTDVYSVMANWGANHCVATFGHVGADLITLASMLRIPVCVHNVEDKDVFRPSAWNG
                     FGQDKEGQDYRACANFGPLYK"
     gene            3062..4495
                     /gene="fucK"
                     /locus_tag="PXD71_001411"
     CDS             3062..4495
                     /gene="fucK"
                     /locus_tag="PXD71_001411"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDL6291526.1"
                     /translation="MPISLIFDCGATNLRTIAINQAGEIVAAHHLPNNTQPDVESPEF
                     HIWDIEEIWSKLMTCAKQTLSQLSEQQRQEIVGISVTTFGVDGALFDKNGKQLYPIIS
                     WKCPRTLPIMENLANYLDVEALYQRNGVGHYSFNTLFKLLWLKENKPEIYQQAESFLF
                     ISSILTYLLTGVQTTDRTMAGTSMMTNIENDSWDKTVLDLLGLTENHFPPMKSAGEVV
                     GTLKGEISQELGLSGQIPVISCGHDTQFAIFGSGAGYNQPVLSSGTWEILMVRTPKAK
                     PQWQFVQNGLTIEFDSQSGYFNPGVQWVASGVMEWFGKRFFSDIANTEHYYRTMIEEA
                     AEVPAGSNGVKLVGNFDGTTAKTGSIIGLSMHITRGEIYRAGLEYMAHRLKEGLDVLQ
                     EVGHFNAESLICVGGGSKNALWNQIRADVLNRPIDVVDFPESTVLGAAMFTFTGAGVF
                     ENPNAAQQAMKPNVKRVEPSSNSQFYK"
     gene            4570..5001
                     /gene="fucU"
                     /locus_tag="PXD71_001412"
     CDS             4570..5001
                     /gene="fucU"
                     /locus_tag="PXD71_001412"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDL6291527.1"
                     /translation="MLKGIHPAISPDLLKVLAEMGHGDELVLSDAHFPAHSIHQNVIR
                     ADGLSVATLLEGISALFEFDQYVEAPLAMMQAVKGDTLDPSVEERYLNAIKKVNGTAP
                     KIERVERFAFYDRAKNAYAVVITGELAKYGNIIIKKGVTPV"
     gene            5061..5714
                     /locus_tag="PXD71_001413"
     CDS             5061..5714
                     /locus_tag="PXD71_001413"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDL6291528.1"
                     /translation="MDRKILARQIIDTCLEMTRIGLNQGTAGNVSVRYKDGMLITPTG
                     TPYEQMTEDSIVFVDGNGKHEEGKLPSSEWQFHLAVYEARPELNAVVHNHAKNCAAVS
                     ILGEPIPAIHYMIACTGTNHIPCVPYAPFGTHQLADYVREGIKHSKAILLAHHGLITA
                     DKTLEKALGIAHEVEVIAEWYLKLLATGKPIPTLNKEQMDIVLGKFKSYGSWVESEQ"
     gene            5776..7305
                     /locus_tag="PXD71_001414"
     CDS             5776..7305
                     /locus_tag="PXD71_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6291529.1"
                     /translation="MQNEVILTMKNISKSFAGVNALKGVELTLRKGEVHALMGENGAG
                     KSTLMKCLLGVYQADEGEIIYKGQPVRFESVLEAQKAGISMIFQELNLIPHLTVAENI
                     FFAREPLKHGLVDKDKMVEESAKLLNFFEIDVDPNDEVRMLSVAKQQMVEIAKALSFD
                     VEVLIMDEPTSALTEKEIDKLFELVDRLRAKGVSIVYISHRMEELKRICNHITIFRDG
                     TYVSNHKFNEITMDEIITKMVGRSLDNHFPPKTAVVTNDIILSIMNAERKGVFEPLNF
                     DLRKGEILGITGLVGAKRTELARAIFGADPLDSGEIFVHENKVSIKDPSDAIKAGIAY
                     LSEDRKLNGVAVRMSIRENITMASMDKVANQIGVISYDEEEKASKTFIDKMEIKTPTI
                     EQKVQNLSGGNQQKVVIGKWLFREAKVMIFDEPTRGIDVGAKYAIYQLLDELAANGVG
                     VIVISSELPEVLGVSDRIIVMREGRMTGMLETKKTNQEEIMHYATGVKNMFAREYGVE
                     K"
     gene            7302..8300
                     /locus_tag="PXD71_001415"
     CDS             7302..8300
                     /locus_tag="PXD71_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6291530.1"
                     /translation="MNEKQKEMLRKMAALAGLVLLIIFFSVTNEYFFTSNNIMTVGLQ
                     TSTIALIGIGATCVILTGGIDLSTGSVVALSGVAAAMIVNAGVPVPLGMILGILVGGI
                     CGLINGILVTRMKLPPFIATLGMMMVARGLALYVTNAAPVSGMPESFANLGNGALFKI
                     VEEGANGLPKVVFAGIPYPVIIMIFITVLFTFALSKLKVGRYLYAIGSNEEAARLSGI
                     KTNIVKIYAYVASGLLSGLTGVILASRLVTAQPNGGVSYELDAIASAVVGGTSLMGGV
                     GTIPGTLIGSFIIGVLRNGLNMNGVSSFVQMIVIGLVIIVAVSLDQLRQSKKAGKK"
     gene            8324..9271
                     /locus_tag="PXD71_001416"
     CDS             8324..9271
                     /locus_tag="PXD71_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6291531.1"
                     /translation="MKKSALFTATLFGLSTLFAGSAMAKSDEIAVIVKSANSTFWQNV
                     RKGAETAGKDIGGQYKVTFQGPEAETAIDAQVNMVDNAVNRGVAGIVLAASDPVALVP
                     AVKKAYESGIPVVLIDSGINSDGKYYQSFLATDNRAAGKLAAEKLLAKVKGGKVAVMS
                     FTQGAASAIERTGGFIDEIKAKADYKLVGPYYSDSDMTKALNQTEDVLGSNPDLAAIF
                     GANEPTAVGMARAVKQKGFAGKIVAVGFDGNSDLQNFVRDGTLDGIVVQSSYQMGYKG
                     VDTIGKLIKGEKVEKVIDTGVVYVTKENIDSEEAKGVLY"
     gene            9327..9578
                     /locus_tag="PXD71_001417"
     CDS             9327..9578
                     /locus_tag="PXD71_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL6291532.1"
                     /translation="MSNTLTDKALVVIAALGGRENIVDVYACITRLRVEVKNTDIVEW
                     DKIKSVGALGVVTSGSVIQSIFGSDAEAYRDEIKRILNI"
     gene            9707..10858
                     /gene="fucO"
                     /locus_tag="PXD71_001418"
     CDS             9707..10858
                     /gene="fucO"
                     /locus_tag="PXD71_001418"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL6291533.1"
                     /translation="MANRLILNETSYHGKGAIQNIVHEVTSRSFKKALVVTDKDLIKF
                     NVAQKVTALLDAAGLPYEIFDEVKANPAVDVIKAGVAKFKASSADYLIAIGGGSPIDS
                     AKAIGIIINNPEFSDVLSLEGVAPTHQKCVPIIAIPTTAGTAAEVTINYVITDEAKKR
                     KFVCVDVHDVPAVALVDPDMMESMPKSLAAATGMDALTHAIEGYITKAAWELTDALHL
                     KAIELIGRSLRGAVEKNPKDVEAMALGQYVAGMGFSNVGLGAVHSMAHPLSAYYDTPH
                     GIANAVLLPYVMEFNKNYTGEKYREIARAMGVKGVDEMSQEEYRNAAIAAVQQLSQDV
                     GIPPKLHQIGVKEADLAALSVDAFNDVCTGGNPRDCTAEELLEVYRIAY"
BASE COUNT         3316 a         2293 c         2495 g         2923 t
ORIGIN      
        1 aatgaaattt cacaaaatat attacaaatt ttacaaaaag ttccctaaat ttaaccgctt
       61 gcaacacaaa atgtgatcgc cttctaaaaa tcaaatttgc ttgctcgaca gtaacgctac
      121 aagcggttaa atttggagaa aaatttacaa aaaggataga ttatgcagct gcgacacaaa
      181 cagataatgg aatatataca acagtttgat gaagctaacg ttcaagaatt ggcggaacat
      241 tgtggggttt cgattgaaac tatccgccga gatttaaata agctagataa agatggatta
      301 ttacatcgca ctcacggtgg agctgtgagc cataaaaagc gagatattgg tcgctcattt
      361 accacccgac aacggctgaa cagtgaggcg aaacgcagta tcgcagaaaa tgtgttggaa
      421 tatttacaac cagaatcggt gattgcttta gatgccagct caacctcttg gaatgtggca
      481 cagcgtattc caaatattcc ttgcaccgtt gtcagcagtt caatgcggat tatctacagc
      541 ctttcacaca aaccgcacat taaaacgatt gccaccggtg gtatttattc cgaaaaatac
      601 gatgccttct atggtccatt atccgagcag ttactgaccc gtttaaaaat tgatcttgcc
      661 attctttctt gtacaggcat tgccgatggg gcgatttggg aatcaaatga aattaatatt
      721 tcccttaaac gtaaattaat tgcgaacagt aaacaggttt ttttattagc cgatcacagt
      781 aaatttgagc gtaaagactt gtttcgtatc gaagatttat cttgcattga taaattattt
      841 atcaaccaac ccccaccgga ctttctcaaa aattattgct tagcacatca aattgaaatc
      901 attatttagt gatttctgcc cgattttatt cagaaataaa acaaatcggg cattcttttt
      961 tcactcattt ttaccaaaat cccccatttt ttgtgatcgc actcacaaaa tttaaactaa
     1021 attcgcattt ttcttcataa ctcatattgc gtatgcataa taaattcaat cgcaaatttt
     1081 tttaaccaat cgggagaact atttatgaca acgttgaaat ctacccctgt aaaaattggt
     1141 atccgcccaa ccattgacgg tcgccgttta ggagtgcgtg aatcacttga agatcaaaca
     1201 atgaatatgg caaaagccgt tgcacaatta attcaatctg aagtccgcca cccaaatggt
     1261 gatttcgttg aatgtgtgat tgctgattcc accatcggtg gtgttgccga agctgcggct
     1321 tgtgcagaaa aattcaaacg tgaaaatgtc ggagcggtga ttaccgtaac cccttgctgg
     1381 tgctacggtt ctgaaaccat tgatatggac ccacatatgc cgaaagccat ttggggcttt
     1441 aacggaacag aacgcccagg tgcagtttat ttagctgctg ctttagcagg tcatagccaa
     1501 ttaggtttac cggcattctc catttatggc acagaagtac aagaagctga cgatcaatct
     1561 attccagacg atgtgcgtga aaaattactc cactttgccc gtgccggctt agcagtggca
     1621 accttacgag gaaaatcata tttatctatc ggctctgttt caatgggtat tgccggttct
     1681 atcgtaaacc aacaattctt ccaagaatat ttgggtatgc gtaacgaata tgtggatatg
     1741 accgaaatta aacgccgttt agatcgccat atttacgatg ccgaagaatt taaattagca
     1801 atgagctggg tgaaaaccta ttgcaaagaa ggtattgatg tcaacgcacc ggaaaatcaa
     1861 cgcagcccgg aagagcgtga aaaactctgg gaagacgtgg tgaaaatgac cattattgcc
     1921 cgtgatttaa tggtgggtaa tcctcgctta gcagagctag gctatagtga agaagcctta
     1981 gggcataatg cgattgctgc cggtttccaa gggcaacgcc aatggacaga ccatttgcca
     2041 aacggcgatt ttatggaagc taccttgaat tcgacctatg actggaacgg cgttcgcgca
     2101 ccatacatta tggcaactga aaacgacagc ttaaatggcg tgaatatgtt gttcggtaac
     2161 ttattaaccg gtcaggcaca aatctttgcc gatgtgcgga catactggag cgaagactcg
     2221 gtagaacgtg tcaccggctg gcgtccggaa agcggtttta tccacttaat caactccggc
     2281 tctgcggcgt tagacggaac aggtcagcat accgatagag caggcaaacc aactattaaa
     2341 ccggcttggg aagtgactga agaagacggt aaacgttgtt tagaaaacac ctgttggtgt
     2401 ccggcagttc acgaatattt ccgtggtggt ttatcttccc aattcttaac caaaggtgga
     2461 atgccgttta cgatgcaccg tatcaacctg atcaaaggta tcgggccggt tttacaaatt
     2521 gccgaaggtt ggtctatcga cttgccggaa aatgtacacg acattttaaa caaacgcgcc
     2581 aatgaaacct ggccgaccac ttggttcgta cctcgcttaa ccggaaaagg cgcatttacc
     2641 gatgtgtata gcgtaatggc gaactggggt gcaaaccact gtgtggcaac cttcggacac
     2701 gttggggcag acttaattac cctcgcctca atgctccgca ttccggtttg tgtgcacaat
     2761 gtggaagata aagacgtatt ccgcccaagt gcgtggaacg gtttcggtca agacaaagaa
     2821 ggtcaagact accgtgcttg tgctaacttc ggaccattat ataaataata cacaatcacc
     2881 ctctcccctt gtgggagagg gacagccaag caattcgttc agaattgaat ggcagggaga
     2941 gggctacaag cggtcgcttt tctctcaaaa tttacaaatc cctctctctg attaaattgc
     3001 taaacgcaat ttaatctatc tctctaaaag ggagatatta ggataagtaa aggagataac
     3061 aatgccaatt tctcttattt tcgactgtgg tgccaccaac ctccgtacga ttgcgattaa
     3121 tcaagcgggt gaaattgtgg cagcccatca tctaccgaat aacacacaac cagatgtgga
     3181 aagcccagaa tttcatattt gggatattga agagatctgg tcgaaattaa tgacctgtgc
     3241 caaacagacg ctttctcaac tttcagaaca acaacggcaa gagattgttg ggatttcagt
     3301 aaccaccttt ggcgtggacg gtgcattatt tgacaaaaac ggcaagcagc tttatccgat
     3361 catttcttgg aaatgcccga gaaccctacc gattatggaa aaccttgcca attatcttga
     3421 tgttgaggcg ttatatcaac gtaatggcgt tggacattat agtttcaaca ccttattcaa
     3481 attattgtgg ttaaaagaga acaaaccaga aatttatcaa caagctgaga gtttcttatt
     3541 catctcatct attttgactt accttcttac aggcgtgcaa acaaccgacc gcacaatggc
     3601 gggaacgtca atgatgacaa acatcgaaaa cgattcgtgg gataaaacag ttttagattt
     3661 attaggctta accgaaaacc attttcctcc aatgaaaagt gcaggagaag ttgtgggaac
     3721 attaaaagga gaaatttccc aagagttagg tttaagcggt caaattccag taatttcttg
     3781 cggacacgat actcaatttg ccatttttgg ctcaggagct ggctataacc aacctgtgtt
     3841 aagttcaggc acttgggaaa ttttaatggt tcgtacccca aaagcaaaac cacaatggca
     3901 attcgttcaa aacggcttaa ccattgagtt tgatagccaa tctggttatt tcaacccagg
     3961 tgtgcagtgg gtagcttcag gtgtgatgga gtggtttggc aaacgtttct tctctgatat
     4021 agccaatact gaacattact accgcacaat gattgaagaa gcggcagaag tgcctgctgg
     4081 ctcaaacggt gtgaaattag tcggcaattt tgatggcaca acagctaaaa caggttcaat
     4141 tatcggctta tctatgcaca tcactcgtgg cgaaatttac cgtgcaggct tggaatatat
     4201 ggctcatcgt ctaaaagaag ggttagatgt attacaagaa gtcggtcatt ttaatgctga
     4261 gagtttgatt tgtgtgggag gtggttctaa aaacgcttta tggaatcaaa ttcgagccga
     4321 tgtcttaaat agaccgattg atgtagtgga tttccctgaa agtaccgtat taggcgcggc
     4381 aatgtttact tttacaggtg ctggtgtatt tgaaaatcca aatgcggctc aacaggcaat
     4441 gaagccaaat gtgaaacggg ttgagccatc gtcaaatagt caattttata aataaagttt
     4501 ctcccctctc tctgatttga ggttcattca gaacatcaaa tcagagagag ggtaaaaaag
     4561 gagttcaaaa tgttaaaagg tattcacccg gcaatttctc cagatttatt aaaagtctta
     4621 gccgaaatgg ggcacggcga tgaattagtg ctttctgatg cacacttccc tgctcactct
     4681 attcatcaaa acgtcattcg tgcagatggc ttaagtgttg ccaccttatt agaaggcatt
     4741 tctgctctgt ttgaatttga ccaatacgtt gaagccccac ttgcaatgat gcaagcagta
     4801 aaaggcgaca cgctcgatcc aagcgttgaa gagcgttacc tcaatgcaat taaaaaagta
     4861 aacggaacag ccccgaaaat agagcgtgta gaacgcttcg ctttctatga tagagccaaa
     4921 aacgcttatg ccgttgtcat tacaggcgag ttagccaaat acggcaacat tattatcaaa
     4981 aaaggcgtaa caccggttta attacaagcg gttaaaaaag cagaattttt tacaaatata
     5041 aaattaaaag gagttcaaca atggacagaa aaatactggc gagacaaatc attgatactt
     5101 gtcttgaaat gacccgaatt gggttaaatc aaggtacagc aggcaatgtg agcgtacgtt
     5161 ataaagacgg tatgctcatt acgccaacag gtacacctta tgagcaaatg accgaagatt
     5221 ctatcgtctt tgttgatggc aacggcaaac acgaagaggg aaaactccct tcaagtgagt
     5281 ggcagttcca tttagcggtt tatgaggctc gccctgaatt aaatgcggta gttcacaacc
     5341 acgctaaaaa ctgtgccgca gtgtctattt taggtgaacc tatccctgcc attcactata
     5401 tgatcgcctg caccggcact aatcacattc cttgtgtacc ttatgccccg ttcggcactc
     5461 accaattagc cgactatgta cgtgaaggca ttaaacacag caaagcaatt ttactcgctc
     5521 accacggctt aatcaccgca gataaaaccc tcgaaaaagc cctcggcatc gcccacgagg
     5581 tggaagtgat cgcagaatgg tatttgaaac tgctcgctac aggaaaacct atcccaacgc
     5641 taaacaaaga acaaatggat atcgtactcg gaaaatttaa gtcctatggt tcttgggtgg
     5701 aaagcgaaca atagcctgcc ataaataaca tacaagcggt gatatttctt aaaattttta
     5761 caaacggagt gcagtatgca aaatgaagta atactcacga tgaaaaacat cagtaaatcc
     5821 tttgcggggg tcaatgcgtt aaaaggtgtt gagcttacgc tgcgtaaggg cgaagttcac
     5881 gcattaatgg gcgaaaacgg tgcaggtaaa tccaccttga tgaaatgctt gctgggcgta
     5941 tatcaagccg atgaaggtga aatcatatat aaaggtcagc ctgttcgctt tgagtcggta
     6001 ttagaggcac aaaaagcggg cattagtatg attttccaag aactgaacct cattccccat
     6061 ttaacggtgg cagagaatat tttcttcgcc cgtgagccac tcaaacacgg tttagtggat
     6121 aaagacaaaa tggtggaaga atcggctaaa ttgctgaatt tctttgagat tgatgttgat
     6181 ccaaacgatg aagtgagaat gttgtcggta gccaaacaac agatggtaga aattgccaaa
     6241 gccctttcat ttgatgtgga agtactgatt atggacgagc cgacctccgc tcttaccgaa
     6301 aaagagatcg ataaattatt tgaattggtc gatagactgc gtgccaaagg ggtgagcatt
     6361 gtctatattt ctcaccgaat ggaagaatta aaacgtatct gtaaccatat tacgattttc
     6421 cgtgatggca cctatgtatc taaccataaa ttcaatgaaa tcacgatgga tgagatcatt
     6481 accaaaatgg ttgggcgttc attggataac catttcccac caaaaaccgc tgtggtgaca
     6541 aacgacatta tcctctcgat tatgaatgct gaacgtaagg gagtgtttga accattgaac
     6601 tttgacttac gcaaaggaga aattctgggc attaccggct tggttggagc aaaacgtact
     6661 gaactggctc gagccatctt cggagcagac ccacttgata gcggtgagat ttttgtgcac
     6721 gagaacaaag tcagcattaa agatccgagt gatgcaatta aagccggtat agcctatttg
     6781 tcggaagacc gtaagctcaa tggtgttgca gtaagaatgt cgattcgtga gaatatcacg
     6841 atggcatcta tggataaagt tgccaatcaa ataggtgtga tttcttacga tgaagaagaa
     6901 aaagcctcaa aaactttcat cgacaaaatg gaaatcaaaa ccccaacgat tgagcagaaa
     6961 gttcaaaact taagtggtgg taaccaacaa aaagtggtaa ttggtaaatg gttattccgt
     7021 gaagcaaaag tgatgatttt tgatgagccg actcggggaa ttgatgtcgg agcaaaatat
     7081 gctatctatc aactgctcga tgagcttgct gccaatggcg tgggtgttat cgtgatctct
     7141 tccgaattgc ctgaagtctt gggagtttcc gaccgcatca ttgtaatgcg tgaaggtaga
     7201 atgacaggta tgctcgagac taaaaaaacc aaccaagaag aaattatgca ttatgcaact
     7261 ggtgttaaaa atatgtttgc tcgtgaatat ggggtggaaa aatgaatgaa aaacaaaaag
     7321 aaatgttaag aaaaatggct gcattggctg gcttagtact attaatcatc tttttcagcg
     7381 taaccaatga atacttcttt acctccaata acattatgac agtagggcta caaacctcta
     7441 ccattgcgtt aattggtatt ggggcaacct gtgtgatctt aaccggtgga attgatttaa
     7501 gtaccggttc agtagttgca ctctccggtg tggcagctgc aatgattgta aatgccggtg
     7561 tacccgtacc actaggaatg attttaggga ttttggtcgg cggtatttgt ggcttaatca
     7621 acggcatttt agtcaccaga atgaaattgc ctccgtttat tgctaccctc ggtatgatga
     7681 tggttgcccg tggtttagca ttgtatgtta ccaatgccgc accagtttcc ggaatgcctg
     7741 aatcctttgc caatctcggt aacggagcat tatttaaaat tgtggaagaa ggggcaaacg
     7801 gcttgccgaa agtggtattt gcaggtattc cttaccctgt gattattatg atctttatta
     7861 ccgtactatt cactttcgct ctctctaaac tgaaagtcgg acgctatctt tacgcaattg
     7921 gttctaacga agaagccgca cgcttatccg gcattaaaac caatattgtc aaaatctatg
     7981 cctatgttgc cagtggctta ctctctggtt taactggcgt tatccttgcc tcccgcctcg
     8041 tcaccgccca gccaaacggt ggggtctctt acgagctgga tgcgattgcc agtgctgtgg
     8101 tgggcggcac atcgcttatg ggcggtgtgg gtactatccc cggcacactc atcgggtcat
     8161 tcattatcgg ggtactccgt aatggtttga atatgaatgg cgtttcttca tttgttcaaa
     8221 tgattgtcat cgggcttgtg attatcgtgg cggtctctct cgaccagctc cgccaatcca
     8281 aaaaagccgg taaaaaataa tccactctta aaggagaatt aaaatgaaaa aatcagcatt
     8341 atttactgcg accctctttg gtttatcgac cttatttgca ggcagtgcaa tggcaaaatc
     8401 tgatgaaatc gctgtgattg tgaaatctgc caactctacc ttctggcaaa acgtgcgtaa
     8461 aggggctgaa actgccggca aagatattgg aggacaatac aaagtcacct tccaagggcc
     8521 ggaagccgaa acagcgattg acgcccaagt gaatatggtg gacaacgccg taaaccgtgg
     8581 cgtggcaggc atcgtactgg ctgcctcaga cccggtagca ttagtgccgg cggtgaaaaa
     8641 agcttatgag agtggcattc cggtggtgct tatcgattcc ggcatcaaca gtgacggcaa
     8701 atactaccaa tctttccttg ccaccgacaa ccgtgcagca ggtaaattag cagcggaaaa
     8761 actgcttgcc aaagtcaaag gcggcaaagt ggcagtgatg agctttaccc aaggggcagc
     8821 atcagcgatt gagcgtaccg gtggttttat tgacgaaatc aaagcaaaag ccgattacaa
     8881 actggtcggc ccgtactact ccgattcgga tatgaccaaa gcgttaaacc aaaccgaaga
     8941 cgtattaggt tctaaccctg atcttgcagc gatcttcggg gcgaacgaac cgactgcggt
     9001 aggaatggca cgagcggtga aacaaaaagg ctttgccggc aaaattgtag cggtgggctt
     9061 tgatggaaac tccgacctac aaaacttcgt gcgtgacgga acactagacg gcatcgtggt
     9121 gcaatcttcc taccaaatgg gctataaagg cgtggacacc atcggcaagc tgattaaagg
     9181 cgagaaagtc gaaaaagtga ttgataccgg ggtggtttat gtcacgaaag aaaacattga
     9241 tagcgaagaa gcgaaaggtg ttctttacta acattcaagt taaacggggt ggggttgccc
     9301 accctttcag ccgaaaggaa gccaatatgt caaataccct taccgataaa gccttagtgg
     9361 tgattgccgc acttggcggt cgagaaaata ttgtcgatgt ttacgcctgt attacccgcc
     9421 tacgtgtgga ggtgaaaaat accgacattg tggaatggga taaaattaaa tctgtcggtg
     9481 cgttaggcgt tgtgacctca ggctcagtta ttcaatctat ttttggtagc gatgccgaag
     9541 cctaccgtga tgaaatcaaa cgtattttga atatttagcc taagcacact cacaacaaaa
     9601 gaatcgcaac aagcggtgaa atttttatca aaatttgcaa aaaaatgacg taatttgacc
     9661 gcttgcaagt taatcgaatt gtttaacttt taagaaggaa aatagtatgg caaatcgtct
     9721 tatcctcaat gaaaccagtt accacggcaa aggagcaatc caaaatattg ttcacgaagt
     9781 gaccagccgg agtttcaaaa aagcccttgt ggtaacagac aaagacctga tcaaatttaa
     9841 cgttgcccaa aaagtcaccg ccttgcttga tgccgcaggc ttgccttatg agattttcga
     9901 tgaggtcaaa gccaacccgg cagtcgatgt gatcaaagcg ggggtcgcaa aattcaaagc
     9961 ctccagtgcc gattatttaa ttgcgattgg tggcggctca ccgattgata gtgccaaagc
    10021 gattggtatt atcatcaata accctgagtt tagcgatgta ctttcattgg aaggtgtcgc
    10081 cccaacccac caaaaatgtg tgccgattat cgccattcca actaccgcag gtacagccgc
    10141 agaagtcacg attaactatg tcattaccga tgaagcgaaa aaacgcaaat ttgtgtgcgt
    10201 ggacgttcac gatgtgccgg cagtggcgtt agtcgatcca gatatgatgg aatctatgcc
    10261 gaaaagcttg gctgccgcaa ccggtatgga cgctctcacc cacgccattg aaggctacat
    10321 taccaaagcg gcgtgggaat taaccgatgc tctacacctt aaagcgattg agctgatcgg
    10381 ccgttcattg cgtggtgcag tggagaaaaa tccgaaagat gtcgaggcaa tggcattggg
    10441 acaatatgtg gctggaatgg gcttctccaa cgttgggctt ggcgcggttc acagtatggc
    10501 acacccgctt tctgcctatt acgacacccc acacggcatt gccaacgcag tattactgcc
    10561 gtatgtgatg gaatttaata aaaattacac cggcgaaaaa taccgtgaaa ttgcccgagc
    10621 aatgggggta aaaggcgtag atgaaatgag ccaagaagag taccgcaacg ccgccattgc
    10681 tgccgtacaa cagctttcac aagatgtcgg cattccgcca aaactgcatc aaatcggcgt
    10741 taaagaagcc gatttagcgg cattatcggt cgatgccttt aatgatgtct gcaccggtgg
    10801 caacccgaga gattgtacgg ctgaggagct gttggaggta tatcggatcg catactaagt
    10861 tctatctttg ttaaatagac aagcggtgat tttttaataa aaatttgcaa tttctgttaa
    10921 aaaaatactg atagtcataa tgcccgattt ctcatattta tggaaaaatc gggcattttc
    10981 gttgcttttt tatcgccaag aaatgtgatt ttgttcacat ttatatc
//
LOCUS       DANLXS010000075        11022 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.075, whole genome shotgun sequence.
ACCESSION   DANLXS010000075 DANLXS010000000
VERSION     DANLXS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.075"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            76..1053
                     /gene="manX"
                     /locus_tag="PXD71_001419"
     CDS             76..1053
                     /gene="manX"
                     /locus_tag="PXD71_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL6291534.1"
                     /translation="MSIAIIIATHGVAAEQLLKTTEMLIGEQEDVAYIDFVPGENAET
                     IMGKYQALVEGPLSHCDQVLFLVDMWGGSPFNAANRFQDGKSGMDVVAGVNIPMLVNT
                     CMARDDVESLEELVEVALEAGSTGIRSLNHQPEVAEVQTATPAQETAAVQQPEIVDDG
                     RRMTIALARIDDRLIHGQVATVWTKESKVNRIIVVNEGVAKDSVRTTMLKSVAPLGVT
                     AHVVDVPKMIRVYNNPEYADDRVMLLFTNPSDVLALIEGGVPLNSINVGGISHKEGKQ
                     MVSHSVAVDQNDIDAFKAIDAKGVELEVRKVSSDSRQYMMDLLKKHNLI"
     gene            1065..1865
                     /locus_tag="PXD71_001420"
     CDS             1065..1865
                     /locus_tag="PXD71_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL6291535.1"
                     /translation="MELSALQIVLLFLVACISGMGSILDEWQTHRPLIACTMIGLVLG
                     DVTTGVIVGGSLELLALGWMNIGAAVAPDAALASIVSTILVIVGRQDISTGIAIAIPL
                     AAAGQVLTYVVRAITVGFQHAADKSIEDGNLSRLDWLHRGALLLQAMRIAIPALIVAL
                     TAGTDAVQTMLNAIPEVVTTGLKIAGGFIAVVGYAMVINMMRAGHLMPFFFAGFVIAG
                     FTDFNLVALGVLGTVIAMVYIHMHPKYNKSQQVVVAAAPKNDLDNRLD"
     gene            1879..2715
                     /locus_tag="PXD71_001421"
     CDS             1879..2715
                     /locus_tag="PXD71_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL6291536.1"
                     /translation="MTTEIKKVTKSDLNAVVRRSNLFQGSWNFERMQALGFAYAMVPV
                     IKRLYPDPNGPGRKEAIKRHSEFFNTQPFVGAPILGVTIAMEEERANGKEIDNAAING
                     IKVGLMGPLAGVGDPIFWGTVRPVFAALGAGLALKGELLGPILFFALFNIVRLAARYY
                     GVVYGYKKGLDVVQDMSGGLLQKLTEGASILGLFIMGALVQKWTSINVPLVLSTIQKQ
                     DGTIEVTTVQSILDSLMPGLLPLLLTFACMWLLRNRVNAIWIILGFFVIGILGAATGA
                     LA"
     gene            2808..3293
                     /locus_tag="PXD71_001422"
     CDS             2808..3293
                     /locus_tag="PXD71_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDL6291537.1"
                     /translation="MTNTILILFILLGLVFAIYDQVFMHKLKGTTLLEIRLKKQKTID
                     TWLLIGLIVLSISYGVQNQIQPFTLYLLATCIILSVYLAFFRSPRLLLKQHGFFFANV
                     FFNYDKIYQVNIAEGKADEKILVIDLHSGRRLLAYVENAEDLQPVVDFFGGYKKESEQ
                     K"
     gene            complement(3379..4170)
                     /gene="tpiA"
                     /locus_tag="PXD71_001423"
     CDS             complement(3379..4170)
                     /gene="tpiA"
                     /locus_tag="PXD71_001423"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL6291538.1"
                     /translation="MARRPLVMGNWKLNGDKAFTKTLIADLKAELHGVEGCDVAIAPP
                     VMYLAHAEASLAGCGCGCSDKGLIALGSQNVDVNVKGAFTGDISPEMLKEFGAKYIII
                     GHSERRTYHKESDEFIAQKFKVLKDAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
                     NAHGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAAKSQAVADQVIIQY
                     GGSVNDANAAELFTQPDIDGALVGGVSLKAPAFAVIVKAAAAAKA"
     gene            4330..5244
                     /locus_tag="PXD71_001424"
     CDS             4330..5244
                     /locus_tag="PXD71_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDL6291539.1"
                     /translation="MKLNYLYSPPQAVGRLKAEYADFIVREELGYELAGEGEFVAVKI
                     RKTNASTLFVGEKLAKFVGISEREMSYAGLKDRHNRKIRVGSLAGNHFELLLRDVAES
                     EELIYRLQQLQAVGFPNYFTEQRFGRDGHNLTQALRWANGEISVKDRKKRSFYLSAAR
                     SEVFNLVVSQRINDCLTQKILENDYVQLAGSNSFFCVKSDEIAETQARLAQQDLLLTA
                     PLIGERSLELECNPAEKAIIEQHAQLVALMQKERMAAARRAMLCKPQNLEWQFEQEGL
                     RLKFFLDSGSYATALVRELILLTEESEA"
     gene            5415..6203
                     /gene="surE"
                     /locus_tag="PXD71_001425"
     CDS             5415..6203
                     /gene="surE"
                     /locus_tag="PXD71_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDL6291540.1"
                     /translation="MRILISNDDGFHAVGIQTLAKALRDAGHIVTIIAPDRNRSAASS
                     CLTLVDPLRVHRFDEYNYAVIAGTPADCVHLALNGLFDGKFAEKFDLVVSGINHGANL
                     GDDVVYSGTVAAALEGRHLPLPSIAVSLVGRKHSSFLDGENHFGTAAQVVLDLLEKME
                     TQRFPSNHVLNVNVPNLPYSELKGTMITRLGERSPAAEIVKQKDPRNADVYWIGVTGE
                     PLDESFGTDFYALNRDCVSITPIQVDMTAHKTIATLKGIFNETV"
     gene            6190..6774
                     /locus_tag="PXD71_001426"
     CDS             6190..6774
                     /locus_tag="PXD71_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL6291541.1"
                     /translation="MKLFETIYDKTMQWSKHRFAAFWLSFVSFIEAIFFPIPPDVMLI
                     PMSMAKPQNAFKYAVYTTLASVAGGVIGYFIGLYATDWVQGVIANWGMQANFDKAKLW
                     FETWGVAVVFLAGFSPIPYKVFTICAGVMQMAFFPFVITAAISRFARFILVAKLSAWG
                     GKKYEQKIRRSIELIGWGTIVLAVVAYIIYTLMK"
     gene            6792..7292
                     /locus_tag="PXD71_001427"
     CDS             6792..7292
                     /locus_tag="PXD71_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL6291542.1"
                     /translation="MKKSLFVLSLISFALVGCSSNSGESTSAVETTEANATWQSADSI
                     QTAPMPASMNQPIATQPTYQQPTYSAPQPISAAPAPMPVSSGSDSSQVETVGNCQVVR
                     DANNAPIYSQITKGCYTESHYTVGKSDTLYLIGYLTGTNANQIAQLNGLNTGSPLKVG
                     QVLRVR"
     gene            7305..7505
                     /locus_tag="PXD71_001428"
     CDS             7305..7505
                     /locus_tag="PXD71_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291543.1"
                     /translation="MNRTLLAFAISCAFLTACSSPTQPQVEDPNMLPAGTLQPIEGSG
                     ASQDSYSWPSDIQSTSMPDSMK"
     gene            7524..8804
                     /locus_tag="PXD71_001429"
     CDS             7524..8804
                     /locus_tag="PXD71_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL6291544.1"
                     /translation="MKKQFILLPLVSAVLAACSSNNPAPVVNASGNNELSPGVMQPVD
                     VSAGAINSSGGWQSDIQSAPMPSSMNTTPVQTMPQPVSVPQPVATVPQPITNPVITEQ
                     PPQPTTTTKIVKKTKTVEKKVDQNFEIPRDANNAPMYNQIQKGFYDGSTYTVRKGDTM
                     FLIAYIVGKDVKEIAALNNMSEPYQLSVGQKLKTGKSATETITVEEKVTVPVEPQITY
                     QQGANGTTYASGGNITGPVKAGTANADVTPVNNGIKATTATAPAVTASVGTVNATEDR
                     TASTIRAIDGTASGAVQSSNTVSAPASSIKWQWPTSGRVISGFSSAEGGNKGIDIAGN
                     KGQDVRAAAAGKVVYAGNALQGYGNLIIIKHTDDFLSAYAHNNTIEVDEQETVKAGQK
                     IATLGSTGTNTNKLHFEIRYKGKSVDPARYLPRK"
     gene            9045..9236
                     /locus_tag="PXD71_001430"
     CDS             9045..9236
                     /locus_tag="PXD71_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291545.1"
                     /translation="MDPTEPSHRIYILLSEDRFQAEVFSTKLSESSMLLEAVKGGYIS
                     KDGKVRLLKKAEGWKIYYE"
     gene            9229..9795
                     /locus_tag="PXD71_001431"
     CDS             9229..9795
                     /locus_tag="PXD71_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL6291546.1"
                     /translation="MSETKIRCDWCKSSEIYMKYHDEEWGKPEFDSLKLFEKICLEGQ
                     QAGLSWITVLKKREAYRQAFHQFNPEKIAKMGEEEIDLLMQNTSLIHHRAKLEAIIKN
                     AKAYITMQQNGEDFSRFIWSFVNYQPQINDVPTLEAVPAHTETSKAMSKALKKRGFVF
                     VGETTCYAFMQSMGLVNDHLNNCVFKHK"
     gene            complement(9842..10072)
                     /gene="acpP"
                     /locus_tag="PXD71_001432"
     CDS             complement(9842..10072)
                     /gene="acpP"
                     /locus_tag="PXD71_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL6291547.1"
                     /translation="MSIEERVKKIIVDQLGAKAEDVKPEASFIEDLGADSLDTVELVM
                     ALEEEFDIEIPDEEAEKITTVQSAIDYVQNNQ"
     gene            complement(10259..10936)
                     /gene="rpe"
                     /locus_tag="PXD71_001433"
     CDS             complement(10259..10936)
                     /gene="rpe"
                     /locus_tag="PXD71_001433"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL6291548.1"
                     /translation="MSKNPYLIAPSILSADLARLGDDVAEVLKAGADLIHFDVMDNHY
                     VPNLTFGPAICKALRDYGIQCPIDVHLMVKPVERLIPDFAKAGADYITFHPEATEHID
                     RNLQLIRDLGCKSGLVFNPATPLSYLDYVMDKVDVVLLMSVNPGFGGQSFIPSTLDKL
                     KEVRRRIDESGYNIRLEVDGGVKINNIADIAAAGADMFVAGSAIFDQPDYQAVIAQMR
                     DELRKVG"
BASE COUNT         3236 a         2280 c         2306 g         3200 t
ORIGIN      
        1 attgtgtgaa ataattatca tattaaaatc tcgccccagt aaataaacaa cttaaaaaac
       61 cgcaaggagt aacaaatgag cattgctatt atcatagcaa cccatggtgt tgctgcagag
      121 caacttctca aaacaactga gatgttgatt ggtgaacaag aagatgttgc ttacattgac
      181 tttgttcccg gcgaaaatgc agaaacgatt atgggcaaat atcaagcatt ggttgaaggt
      241 ccactatcac actgtgatca agtgctgttt ttggtggata tgtggggagg aagcccattc
      301 aacgccgcaa accgattcca agatggaaaa tcgggtatgg atgttgttgc gggtgtgaat
      361 attcctatgt tagtgaacac ctgtatggct cgtgatgatg tggagtcgtt ggaagaattg
      421 gtggaagtcg cattagaagc cggctcaacc gggattcgtt cacttaatca ccaacctgaa
      481 gtggctgagg tgcaaaccgc tactcctgca caagaaactg cagctgttca acagcctgaa
      541 attgttgatg acggacgtcg tatgaccatt gcccttgccc gtattgatga ccgcttgatt
      601 cacggtcaag ttgcgacggt ttggacaaaa gaaagtaaag tcaatcgcat tattgtggta
      661 aacgaaggtg tagcaaaaga tagtgtacga actactatgc taaaaagtgt tgctcctctt
      721 ggcgtaacgg ctcacgttgt tgatgtacct aaaatgattc gtgtctataa caaccctgaa
      781 tatgccgatg atcgtgtaat gttactcttt accaacccaa gtgatgtctt agccttaatt
      841 gaaggcggtg taccactcaa tagcattaac gtgggcggta tttcgcataa agaagggaaa
      901 caaatggtta gccattctgt ggcggttgat caaaatgata ttgatgcctt caaagcaatt
      961 gatgcgaaag gcgttgaatt agaagtacgc aaagtctcaa gcgatagccg tcagtatatg
     1021 atggatttat tgaaaaaaca taacttaatc taaaggaaat tactatggaa ctttcagcat
     1081 tacaaattgt gcttttattc ctcgttgcct gtatctcagg tatgggctca attcttgatg
     1141 aatggcaaac ccaccgtccg ttaattgcct gtacgatgat aggcttagtg cttggtgatg
     1201 tgactaccgg cgttattgtt ggtggctcat tagagttatt agctttaggt tggatgaaca
     1261 tcggtgccgc tgttgcacca gatgcggcat tagcctctat tgtctcaacc attttagtca
     1321 ttgtaggtcg tcaagatatt tcaactggta tcgccatcgc tattccgctt gcagctgccg
     1381 gtcaagtatt aacctatgtt gtgcgtgcaa tcaccgtagg tttccaacac gcagcagata
     1441 aatcaattga agacggtaac ttaagccgtt tagactggtt acatcgtggt gctttattgc
     1501 ttcaagcaat gcgtattgcc attccggcat taattgtggc attaacagca ggtacagatg
     1561 cggttcaaac aatgttaaat gccattcctg aagtggttac aaccgggctg aaaattgccg
     1621 gtggcttcat cgccgtggtg ggttatgcga tggtgatcaa tatgatgcgt gcaggtcatt
     1681 taatgccgtt cttcttcgca ggctttgtaa ttgcaggctt taccgatttc aatcttgttg
     1741 cccttggggt attaggtacg gttattgcaa tggtttatat ccatatgcac cctaaatata
     1801 acaaaagcca acaggtagtg gtggcagctg caccaaaaaa tgatttagac aaccgtttag
     1861 actaagaggg catttaaaat gacaactgaa atcaaaaaag taacaaaaag cgatttgaat
     1921 gccgttgtac gtcgttcaaa cttattccaa ggttcgtgga actttgagcg tatgcaggct
     1981 cttggttttg cgtatgcgat ggtaccggtt atcaaacgtt tatatccgga tccaaatggc
     2041 ccgggtcgca aagaagcaat taaacgccat tctgagttct ttaacaccca gccattcgtc
     2101 ggtgcaccga ttttaggggt aaccattgct atggaagaag aacgtgccaa cggtaaagaa
     2161 atcgacaatg ccgcaattaa cggtattaaa gttggtttaa tgggaccatt agccggtgtg
     2221 ggtgacccaa ttttctgggg aactgttcgc cctgtatttg ccgcattagg agcaggctta
     2281 gccttaaaag gtgagctttt aggcccaatc ctgtttttcg ccttatttaa catagtgcgt
     2341 ttagccgcac gctattatgg cgtagtgtat ggctataaaa aagggttaga tgttgtacaa
     2401 gatatgagcg gcggtttatt gcaaaaatta accgaaggtg cttcaatctt aggtctgttt
     2461 attatggggg cattggtaca aaaatggacg agcattaacg ttccgcttgt gctttcaacc
     2521 attcaaaaac aagacggtac catcgaagtt accacagtgc aaagcattct cgacagctta
     2581 atgccgggcc tactcccatt attattaacc tttgcttgta tgtggttact tcgtaatcga
     2641 gtaaatgcta tctggattat cctcggtttc tttgttatcg gtatcctcgg cgctgcaacc
     2701 ggtgcattag cttaattgca aaattttctg tgaaaacgac cgcttgaact tgaacattca
     2761 agcggttttt tatacgcttt agcccaaata aattcacttg cttaattatg accaatacca
     2821 tccttattct ctttatttta cttggcttag tattcgccat ttatgaccaa gtttttatgc
     2881 acaaactcaa aggcacaacc ttactggaaa tccgtttaaa aaagcaaaaa acaattgata
     2941 cttggttatt gattggttta atcgtcttgt caattagcta tggcgtacaa aatcaaatcc
     3001 agccttttac actttacctg ctggcgactt gcattattct gtcggtctat ctcgcctttt
     3061 tccgttcccc tcgcttgttg ttaaaacagc acggtttttt ctttgcgaat gtctttttta
     3121 attacgacaa gatttatcaa gtcaatattg cagaaggaaa agcagacgaa aaaattttgg
     3181 taattgattt acatagcgga agaaggctgc ttgcctatgt tgaaaatgcg gaagatcttc
     3241 aaccggtagt ggattttttt ggcggttata aaaaggaaag tgagcagaaa tagtttgcga
     3301 acaaaacaca agcggtcatt tttccctgaa attttgcaaa aaactgagga aaaatgaccg
     3361 cttgtaaaat cggttaaatt aggctttagc agccgctgcc gctttaacga tcaccgcaaa
     3421 agcaggcgct ttaagtgaaa caccgcctac taatgcaccg tcaatatccg gttgagtgaa
     3481 taactctgct gcattggcat cgtttacaga gccaccgtat tgaatgatca cttggtcagc
     3541 cactgcttgc gatttagctg caatatggtc acgaataaat ttatgcactg cttgtgcttg
     3601 tgccggagtc gctgatttgc cggtaccgat tgcccaaatt ggttcgtaag caataactgc
     3661 accgttgaat gcttccacac cgtgcgcgtt gatgaccgca tcaatttgtc ttgcacacac
     3721 ttcttcagtt ttacctgctt cattttcggc ttcagattca ccgatacata atacaggcac
     3781 taagccggca tcttttaata ctttgaattt ttgagcaata aattcatcgc tttctttgtg
     3841 gtaagtacgg cgttctgaat gcccgataat aatatatttt gccccaaact cttttaacat
     3901 ttcaggtgaa atatcaccgg tgaatgcccc ttttacgttc acatcaacgt tttgtgaacc
     3961 aagtgcgatt aagcctttat cactgcaacc acagccacaa ccggcaagtg atgcttcagc
     4021 gtgtgccaaa tacatcaccg gtggagcgat tgctacatca cagccttcaa cgccatgtaa
     4081 ttctgctttt aaatctgcaa ttaaggtttt agtaaacgct ttatcaccgt ttaatttcca
     4141 gttacccatt acaagtggac gacgtgccat tttgtttctc ctatatgaag ttgaacttaa
     4201 atataaacgt tgcaaactat accaaatttt aatgtcgttt tgttgttttt attttgcttt
     4261 ttctgatcta gatcgaaagg tttaacgtat aatgccgaaa tcttattttt tatgttattc
     4321 gagaagtgaa tgaaactgaa ttatttatac tctcctcctc aagctgttgg gcgactaaaa
     4381 gccgaatatg cagactttat tgtgcgtgaa gaactgggct acgaactagc cggcgaaggg
     4441 gaatttgtgg cggttaaaat ccgcaaaacc aacgccagca cgctgtttgt gggggaaaag
     4501 ttagccaaat ttgtcggtat ttccgagcgt gaaatgagtt atgcaggctt aaaggaccgc
     4561 cacaaccgca aaattcgtgt tggttcgctc gccggcaatc attttgaatt attgctacgt
     4621 gatgtagcag aaagcgaaga gttgatttac cgtttgcaac agttgcaagc ggtcggattt
     4681 cccaattatt ttaccgaaca acgtttcggg cgagacggac ataacctgac tcaagccttg
     4741 cgttgggcaa acggggaaat tagcgtgaaa gatcgcaaaa aacgcagttt ctacctttcc
     4801 gctgcccgct cggaagtgtt taatttagtg gtttcacagc ggattaatga ctgcttaacc
     4861 caaaaaatct tggagaatga ttacgttcaa cttgccggct ctaacagctt tttctgcgtg
     4921 aaatcggacg aaattgctga aactcaagcc cgtttagcac agcaagatct acttctcacc
     4981 gctccgctga taggcgaaag atcgcttgaa ttagaatgca atcctgctga aaaagccatc
     5041 atagagcaac acgctcaatt ggttgcctta atgcaaaaag agcgtatggc agccgctcgg
     5101 cgagcaatgc tttgtaagcc acaaaatctg gaatggcaat ttgaacaaga agggctacgg
     5161 ttaaaattct ttttagattc aggcagttat gccactgcac tggtgcgaga gttaattctg
     5221 cttaccgaag agagcgaagc ttagtccata acacataaca agcggtaaga ttttaatcat
     5281 tttttgcaat ttgcaaaact ttaacaaaat cttaccgctt tttgcacttt tagactaaaa
     5341 aaactgtcga aaaattgtta gaattgcgat ctttgtattt agagtctatc aagatgtaag
     5401 atctaaagga aattatgcga attttaatta gtaatgacga tggttttcac gctgtcggca
     5461 ttcaaacttt agccaaagcc ttgcgagatg caggccatat cgtcaccatt attgcaccgg
     5521 atcgtaaccg cagtgcagct tcaagctgcc taaccttagt agatccactt cgggtgcatc
     5581 gttttgatga atataattat gcggtgatcg ccggcacacc tgccgattgc gtgcatttag
     5641 ccttaaacgg cttgtttgac ggcaaatttg cagaaaaatt tgatttggtg gtctccggca
     5701 ttaatcacgg ggcaaattta ggcgatgatg tggtttactc cggtacggta gcggcagcat
     5761 tggaaggccg ccacttgcca ttaccaagta ttgcagtatc actggttggg cgtaaacact
     5821 cttcgttttt agacggtgaa aatcattttg gaacggcagc acaggtagtg ttagatttat
     5881 tggaaaaaat ggaaacgcaa cgttttccat caaaccacgt tctcaatgtt aatgtgccga
     5941 atttacctta ttcagagcta aaaggcacaa tgatcacccg cttaggtgaa cgttcacccg
     6001 ctgcggaaat tgtgaagcaa aaagatcctc gtaatgctga tgtctattgg attggtgtaa
     6061 caggtgaacc actggatgaa agttttggta ctgattttta tgcattaaac cgagattgcg
     6121 tttctatcac accaattcaa gtggatatga ccgctcataa aacgatagca accttaaaag
     6181 gcatttttaa tgaaactgtt tgaaacaatt tatgacaaaa caatgcagtg gtctaagcac
     6241 cgcttcgctg ccttttggtt gagttttgtc agttttatcg aggcgatctt tttcccgatt
     6301 ccgccggatg tgatgttgat tccgatgtcg atggcaaaac cgcaaaatgc gtttaaatat
     6361 gcagtttaca ctacccttgc ttcggttgcc ggtggtgtta ttggttattt tattggctta
     6421 tatgctaccg attgggtgca aggcgtcatc gcaaattggg gaatgcaagc aaactttgat
     6481 aaagcaaaat tgtggtttga aacttggggt gtcgcggtag tatttttagc cggtttttcg
     6541 ccgattccct ataaggtatt taccatctgt gccggtgtaa tgcagatggc gttcttcccg
     6601 tttgtgatta ctgctgcgat ttctcgcttt gcccgtttta ttttggtagc aaaactctct
     6661 gcttggggtg gcaaaaaata tgaacaaaaa attcgccgct caattgaatt aattggttgg
     6721 ggaacaatcg ttttagccgt tgttgcctat ataatttata cattaatgaa ataaatttta
     6781 aggatataaa aatgaaaaaa tcactttttg ttttatctct gatttctttt gctttagtgg
     6841 gttgttcaag caattcgggt gaatccactt ctgcggtaga aacaacagaa gcaaatgcaa
     6901 cttggcaatc agcagatagc attcaaactg caccaatgcc tgcctcgatg aatcaaccta
     6961 ttgccactca gccaacctat cagcagccga cttactctgc accacaacca atttcggcag
     7021 caccagcacc aatgcctgtt tcaagtggta gtgatagctc tcaagttgaa acagtaggga
     7081 actgccaagt ggtgcgtgat gcgaataatg ctcctatcta ttcacaaatt accaaaggct
     7141 gctacacaga aagtcactat acggtaggta aaagtgatac gctttattta atcggttatc
     7201 taaccggcac caatgccaat caaattgcgc agctaaatgg tttaaatacc ggctctccac
     7261 tgaaagtcgg gcaagtctta cgtgttcgtt aataggcaga gaaaatgaac agaacccttt
     7321 tagcatttgc aatttcttgt gcatttttga ccgcttgtag ctcgcctact cagccacaag
     7381 ttgaagatcc aaatatgtta cctgcaggca cattacaacc gattgaaggc tcaggcgcaa
     7441 gccaagacag ttacagttgg ccgtctgata ttcaatcaac gtcaatgcct gacagtatga
     7501 aataatttta ggacaatcat ttaatgaaaa aacaatttat tcttctccct ttagtctctg
     7561 ctgttttggc agcctgttct tcaaacaatc cagcaccggt ggtgaatgct tccggtaata
     7621 acgaattaag cccgggcgtg atgcagcctg ttgatgtgag tgcaggtgca attaattcaa
     7681 gcggtggctg gcagtctgat attcaatctg caccaatgcc aagctcgatg aataccactc
     7741 cggtgcaaac aatgccccag ccggtttcag tgccacaacc tgtggcgact gtaccgcagc
     7801 caattaccaa tccggtgatt accgaacaac caccacagcc gacaaccacg accaaaatcg
     7861 tgaaaaaaac gaaaacggtc gagaaaaaag tggatcaaaa ttttgagatc ccgcgtgatg
     7921 ccaacaatgc cccaatgtac aaccaaattc aaaaagggtt ctacgatggc tctacctata
     7981 ccgtacgcaa aggcgacaca atgtttttga ttgcctatat tgtgggtaaa gatgtaaaag
     8041 aaatcgccgc tcttaataat atgagcgaac cttaccaatt aagcgtaggg caaaaactca
     8101 aaaccggcaa atctgcaacg gaaactatta ccgtggaaga aaaagtcacc gtaccggttg
     8161 aaccacaaat tacctaccaa caaggtgcaa acggcacaac ctatgcttct ggcggtaata
     8221 ttactgggcc ggtaaaagca ggcacagcca acgctgatgt cacgccggtt aataacggta
     8281 ttaaagcaac aactgcaact gctccggcag taactgcttc tgtcggtaca gtaaatgcaa
     8341 cagaggaccg taccgcctcc accattcgtg ctatagatgg cacagcaagt ggtgccgtac
     8401 aaagtagtaa tactgtttct gcaccagctt catcaattaa atggcaatgg ccaaccagcg
     8461 ggcgtgtaat ttcaggcttc tcctcagcag aaggaggtaa taaaggcatt gatattgccg
     8521 gcaataaagg acaagatgta cgtgctgccg ccgcaggtaa agtggtatat gccggtaatg
     8581 ccttgcaggg ttacggtaac ttaatcatca tcaaacatac cgatgatttc ttaagtgctt
     8641 atgcacacaa caatactatt gaagttgatg agcaagaaac ggtgaaagcc ggtcagaaaa
     8701 tcgcgacttt aggtagtaca ggaaccaata cgaataaatt acattttgaa attcgttaca
     8761 aaggaaaatc tgtcgatccg gctcgttatt taccaagaaa ataagagtaa ctttttagca
     8821 taatccgacc gcttgtgtac tacaagcggt caatttttag gaaaattatg caaatgaaaa
     8881 aatatatcgc acacatgata agttcgttac tgctgatagc ttgttcggac taccgagcaa
     8941 caccaactag cccaagcgat gcttacactt gctctccttc ttttcgctac tctttcttaa
     9001 aaaaaacgtg cataccttta ttcagtatag ccgacattca attattggat ccaaccgagc
     9061 cttcacatcg catttatatt ttgttatctg aagatcgatt tcaagccgaa gtcttctcaa
     9121 cgaagctctc tgaatcttct atgttattgg aggcggttaa aggaggctat atttcaaaag
     9181 acggaaaagt tcgtttactc aaaaaagctg aaggttggaa aatttactat gagtgagact
     9241 aaaatacgtt gtgattggtg taaaagcagt gaaatttata tgaaatatca cgacgaagaa
     9301 tggggaaaac cggagtttga cagcctcaaa ctgtttgaga aaatctgcct tgaagggcaa
     9361 caagccggac tttcttggat tacggtgttg aaaaaacggg aagcctaccg ccaagcattc
     9421 catcagttta atcctgaaaa aattgccaaa atgggtgaag aagaaattga tttgttaatg
     9481 caaaacacta gcttaatcca ccaccgagca aagttagaag ccattataaa aaatgcgaaa
     9541 gcttatatca caatgcagca aaatggcgaa gacttcagtc ggtttatttg gtcttttgtg
     9601 aattatcagc cgcaaattaa tgatgtacca acacttgaag ctgttcctgc tcatacagaa
     9661 acctcaaaag caatgtcaaa agcgttgaaa aaacgtgggt ttgtgtttgt aggcgagact
     9721 acttgctatg cttttatgca gtcaatgggg ctggttaatg accatttgaa taattgcgtg
     9781 ttcaaacata aataaacaca aaaaattagg cgaccatttg gtcgcctaac attaattcag
     9841 attattgatt gttttgaacg taatcaattg ctgattgaac tgtagtaatt ttttcagctt
     9901 cttcatctgg aatttcgata tcgaattctt cttctaaagc cattactaat tcaacagtgt
     9961 ctaaagaatc tgcacctaaa tcttcaatga atgaagcttc tggttttaca tcttctgctt
    10021 tcgcgccaag ttgatcaaca atgatttttt ttacgcgttc ttcaatgctc attttgtttt
    10081 tcctatagtg aatatcgcat aagtgcgaga gttaaatagt gtaatcgaaa taagtttaaa
    10141 tacaaccttt tataaaaagg tcaaaccaga ctattcctat acaccagaat atatctgttt
    10201 ttccattcta tcattatgtt tgtgtttaat ctatagagat aaatcaaatt tcatttgatt
    10261 accctacttt ccgtaattca tctcgcattt gtgcaatcac tgcttgataa tctggctggt
    10321 caaaaatcgc agaacctgcc acaaacatat ctgctcctgc ggctgcaatg tcggcaatgt
    10381 tatttatttt cacaccacca tcaacctcta agcgaatgtt ataaccgctc tcatcaatac
    10441 gacgacggac ttctttcaat ttatctaagg tggacggaat aaatgattgt ccgccaaagc
    10501 ccggattcac agacatcaat aaaaccacgt caactttatc catcacataa tctaaatagc
    10561 tcaatggcgt tgccgggtta aacaccaagc ctgatttaca acctaaatca cggataagct
    10621 gtaaatttcg atctatatgt tccgttgctt cagggtggaa tgtaatataa tccgctccgg
    10681 ctttggcaaa atccggaatc aaacgctcaa ccggttttac cattaaatgc acatcaatcg
    10741 ggcattgaat gccgtaatca cgtaaggctt tgcagatcgc cggcccgaag gtcaaattcg
    10801 gcacgtagtg gttgtccatt acatcaaagt gaattaagtc tgcaccggct tttaacacct
    10861 ctgccacatc atcacccaag cgagctaaat cggcagataa aatagacggt gcaattaaat
    10921 aaggattctt actcatattt cctcactatc aaaaaataaa tctacgttag attaccataa
    10981 ttttaacagc gaaaagagtc tctcttccta aaaatccgtt tt
//
LOCUS       DANLXS010000076        10865 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.076, whole genome shotgun sequence.
ACCESSION   DANLXS010000076 DANLXS010000000
VERSION     DANLXS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.076"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            37..432
                     /locus_tag="PXD71_001434"
     CDS             37..432
                     /locus_tag="PXD71_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6291549.1"
                     /translation="MKKLMIFATTAMIVSNLAHAAGEQSDAREAHESTVKTSTVKYSC
                     QNGKKLSVKYGFNKQGIPTYAEAKLSGKKRFMPINLYTTDATGTNFGDENNFSLYGDP
                     MEFTNHRKASVNIQSPASEILYKGCTPQK"
     gene            complement(642..2801)
                     /locus_tag="PXD71_001435"
     CDS             complement(642..2801)
                     /locus_tag="PXD71_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL6291550.1"
                     /translation="MQIKQTVLAIAITPFISQLSLAQETKISSIELDTIEVNEQQENL
                     LKQQAVMSNEDLVRYSTDMGVPQTGRFGPQGFNIRGVEGNQVAVTVDGISQAASNNYA
                     TFSRYSYYNKSRPEIDTELMSDVTIEKGSSTKVGNGALGGSVNYKTKTVSDILMKGRQ
                     FGGMLKYTYNGQNKENIETAGIAADGQYADALVMYSHRQGHEAQTTSKGPDIMGSGRG
                     LPNPYKNNQYSYLAKLGLKWNDENRLELKTSKQRSKNAGQELSYSTVTLRDFLDVQEI
                     ETYGVTYLYTPKNSILENIKVAVTKQDTEISGINDQITIARGTKTDNTKRAFNTKNVE
                     GSLGVTFKPVDLGNTEHKLSTELGYGVSDFSFDMQTYTAGITPTDQNMQSPAKTKSFH
                     ILMGDSILLTQNLSAHLNARLSSQKLHSDDNSLSDKTFNNWAGDIGLNYQLSPVWQIG
                     YKLSRGFRTPTASEMFFNREVQGRGASQTFLANTNLKPETSLGNEISLVGNGSLGSLS
                     VAAYHTHYRNLIDLATIGNNIYQSQNVNRAKIKGVDINGTLDLNTAWNAIPQGFELNG
                     GFGYARGKRNDGTDLLTVQPLKALFGIGYRAVNNDWAIHLNGTYTQGKKAKYAQQYAI
                     LANTGYLSYDKSLTTYPYLSNSATVFDLVAHKKFGERAIVKVGVYNLFNKKYWTWDSL
                     RTIGITSPGVANSITGEGLNRYLAPERYVMASVEFKF"
     gene            2908..3774
                     /locus_tag="PXD71_001436"
     CDS             2908..3774
                     /locus_tag="PXD71_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6291551.1"
                     /translation="MEYKMRTLLFSFIALSMCLNTTKAAEKIVSTAGYASEIVAALGK
                     ADKLVGVDTTSVKPQTIMEKKPKIGYRRQLSAEGILSLTPDLIILAPDAGPNAVIEQI
                     KASGIPLLHLGEKPSLAGIREDIQQIAKAIGAESEAKQLTETILADEKKLADIRSQHS
                     TDKEVLVLIDTASQGIFGLGKKSVGDHFLTLLNLNNSFNAEGNKPLANEALAASNADV
                     ILLASRNQAKESATISKLAESSPQYAQLTNTQAGKKGCVFAINILDALGFGPYTAQYA
                     HQILTTIQPCLK"
     gene            3785..4771
                     /locus_tag="PXD71_001437"
     CDS             3785..4771
                     /locus_tag="PXD71_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6291552.1"
                     /translation="MKLHLTLIFTLIISLVICTHLGAMSLPLSAWHCNNAECESAAYI
                     LWQIRLPRLLGALFIGAALAISGATMQGLFRNPLVDPGIIGVSSGAALAAALYIVVIQ
                     PLLPESISYYALPIAAFLGGWLVTFMLYLLSQRNGQLHIAIMLLIGIALAAFSGALTG
                     ILIYVADDNQLRSLTFWSMGSLAGMNWQMVAMMAAVVFICSPLIWREHRALNALTLGE
                     EDAQYIGFDIAKVKRRLVFYVALLTGTSVACVGAIGFVGLVIPHLMRLLGGSNHRHLL
                     LNSLVCGAILLMIADTLARTLAAPAEIPIGIFTALFGAPFLAVMVWKSGKHI"
     gene            4771..5577
                     /locus_tag="PXD71_001438"
     CDS             4771..5577
                     /locus_tag="PXD71_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6291553.1"
                     /translation="MSNLLTCQNLSIVHQQKKLLEIQEIAFEQGKFTAIIGPNGAGKT
                     TLLKALSGNNAQTNSAVFYQHQRLTDLNGYALAQKRAMLSQQSQIAFPLKVEELVQLG
                     REPYRQSEFAKFNEKITAWAMETMSLTALKERSSIQLSGGEQHRAHIARVLAQLVPEP
                     NADLSGSWLLLDEPTNHLDIHHQYQLFSLLQKLKQQGLTIIAILHDPALAINQADHIL
                     MLKNGEKFAEMSAKAIAESCLLDELYEMKMGCRYCPHSGQYYLCPALAES"
     gene            complement(5598..6236)
                     /gene="purN"
                     /locus_tag="PXD71_001439"
     CDS             complement(5598..6236)
                     /gene="purN"
                     /locus_tag="PXD71_001439"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL6291554.1"
                     /translation="MKKFVVLISGNGSNLQAMIDAQKSADTSGQICGVICNKADAYGL
                     IRAKQAGIPTFVFSRKDYQSNVEMDLAIAEQIEQLGAELIVLAGYMKILTPEFTQHFA
                     GKILNIHPSLLPKYPGLNTYQRAIEAGESEHGTTIHFVNEEVDAGAVVLQAKVPIYPE
                     DEIEDVMARVVEQEHRYYPLVIEWFCSGRLVSQHGKAYLDGKLLPACGYAED"
     gene            complement(6343..8500)
                     /locus_tag="PXD71_001440"
                     /pseudo
     CDS             complement(6343..8500)
                     /locus_tag="PXD71_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(8554..9390)
                     /locus_tag="PXD71_001441"
     CDS             complement(8554..9390)
                     /locus_tag="PXD71_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDL6291555.1"
                     /translation="MLPRISSFLASLFMAFFLLGCSVSTQSLNEQNVDSIGQIIDLNT
                     NQTISFTELLSRLSSQDYVLVGEEHTQLIHHQIELYLFNQLTKKAKLHLIALEMLNVD
                     QQPAIDRIQFNRPVNLSATDLREAIQWQKWDWKMYQDLVVESLGSNSRVIATNLTDKE
                     VEILLNGAEPLKGSVSTSSEVKQKIAQLLLSMNHGMPYPMENMVAVQQFRDRRMAEKL
                     VKNALSTSLLIAGNHHVRKDLGVPLHIAEYDRTKKVAVLMLKTEREEITSSQADYLWV
                     TQ"
     gene            complement(9548..10810)
                     /locus_tag="PXD71_001442"
     CDS             complement(9548..10810)
                     /locus_tag="PXD71_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL6291556.1"
                     /translation="MLKRDMNIADYDAVLWQAIQDEDRRQEEHIELIASENYASPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKALFGADYANVQPHSGSQAN
                     AAVYMALLNPGDTILGMSLAHGGHLTHGASVSFSGKIYKAEQYGITDEGLIDYEALRK
                     QAQEVKPKMIVAGFSAYSQVMDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPNPLP
                     HAHVVTTTTHKTLAGPRGGLILSASGDEEMYKKLQSAVFPAGQGGPLMHVIAAKAVCF
                     KEALEPEFKAYQQQVVKNAKAMVEVFKQRGFDVVSNGTENHLFLVSFVKQGLTGKAAD
                     AALGAANITVNKNSVPNDPQKPFVTSGIRVGTPSVTRRGFKEAEVTALAGWMCDVLDS
                     IGKDNHEQVIAETKAKVLDICKRLPVYA"
BASE COUNT         3227 a         2305 c         1999 g         3334 t
ORIGIN      
        1 tctgaactaa gataacgctt atttaaagga aaatatatga aaaaattgat gatattcgca
       61 acaactgcca tgattgtaag caatttagcc catgcagcag gtgaacaaag tgatgcaaga
      121 gaagcccacg aaagcaccgt aaaaacctca actgttaagt atagctgcca aaacggcaaa
      181 aaattaagcg tgaaatacgg cttcaataaa caaggcattc caacctatgc tgaggcaaaa
      241 ttaagcggta aaaaacgttt tatgccgatc aacctataca cgacagatgc aaccggcact
      301 aactttggtg acgaaaacaa tttcagcctt tacggtgatc caatggaatt taccaaccac
      361 cgtaaagcat cagtaaatat ccaaagcccc gcaagtgaaa ttttatataa aggttgcaca
      421 cctcaaaaat agataaaaag taaaatgagt ccgctctgat aagtggcttt ttaatagctg
      481 tttattgcag ataggaaagc attttgtttt tatctactat ccagaaacaa gcggttaaat
      541 ttgtttaatt ttttacaaaa aatgggccaa aattaaccgc ttgtttatta ctataaagaa
      601 ataagtacgt aaaaattaac tcttacattt aatgcttatc tctaaaattt aaactccact
      661 gatgccatta catagcgctc tggagcaagg taacgattta agccttcgcc agttatactg
      721 ttggcaactc caggactagt tataccaata gttcttaaac tatcccaagt ccaatatttt
      781 ttattaaaga gattataaac cccaacttta acaatcgctc tttctccaaa ctttttgtgt
      841 gcaactaagt caaatacggt ggctgaatta cttaagtatg ggtaagtggt taatgattta
      901 tcgtaactta aatacccagt attggctaaa atagcatact gttgtgcata ttttgctttt
      961 ttgccttgtg tgtatgtgcc gtttaggtgt atcgcccagt cattgttcac agctcgataa
     1021 cctatgccga acaatgcttt taacggttga accgtgagta agtcggtacc atcattacgt
     1081 ttgccacgag cataaccaaa tccaccatta agctcaaagc cttgtggaat tgcattccac
     1141 gctgtgttta agtctaaagt tccgttaata tcaacacctt tgattttagc tcggttaaca
     1201 ttttgcgatt ggtaaatatt gttaccaata gtggcaagat caatcagatt tctatagtgg
     1261 gtatggtaag cggctacgct caagctacct aatgaaccat tacctaccag tgaaatttca
     1321 ttacctaagc ttgtttccgg ttttaagtta gtatttgcta agaaagtttg gcttgctcca
     1381 cgcccttgaa cttcacggtt aaagaacatt tcacttgcgg taggtgttct aaaaccacgg
     1441 ctgagtttat agccaatttg ccatacagga gagagctgat aatttaaacc aatatctccc
     1501 gcccaattgt taaaggtttt atctgacaaa ctattatcat cagaatgtaa tttctgactg
     1561 ctcaatcgag catttaaatg agcagataga ttttgtgtca gtaaaataga gtctcccatc
     1621 aaaatatgga aagacttggt ttttgccgga gactgcatat tttgatcggt tggcgtaata
     1681 cctgctgtat aggtttgcat atcaaaactg aaatcagata caccatagcc taattccgtt
     1741 gacaatttat gttcggtatt acctagatca acaggcttga atgtaacacc taagctacct
     1801 tcaacatttt tagtattaaa agcccgtttg gtattatctg tttttgtacc acgggcaata
     1861 gtgatttgat cgttaatacc tgaaatttca gtatcctgtt tggttaccgc aactttaata
     1921 ttttcaagaa tactgttttt tggagtgtat aaataagtaa cgccataagt ttcaatttcc
     1981 tgcacatcca aaaaatcacg taatgtgacg gtagaataag aaagctcttg accggcgttt
     2041 ttactgcgtt gcttagaagt ttttaattca aggcgatttt catcattcca ttttagccct
     2101 aattttgcca agtagctgta ttgattattt ttgtaaggat ttggtaaacc acgaccactt
     2161 cccataatat ccggaccttt agacgttgtt tgtgcttcgt gtccttgacg gtggctatac
     2221 ataaccaaag catccgcata ttgaccatcc gcagcaatac ctgccgtctc aatattctct
     2281 ttattttgac cgttataggt atatttcaac atcccaccaa attggcgacc tttcattaaa
     2341 atatcactta cggttttagt tttatagttc acagagccgc ctaatgcccc attgcctact
     2401 tttgtgcttg agcctttttc aatggttaca tcgctcatta attcagtatc gatttcaggg
     2461 cgagatttat tgtagtaact atagcgacta aaggtggcat agttatttga tgctgcttgc
     2521 gatatgccat caactgttac tgccacttga ttaccttcaa caccacggat attaaaccct
     2581 tgtgggccaa agcggcctgt ttgcggaacg cccatatcag tactataacg tactaaatct
     2641 tcattgctca tcaccgcttg ctgtttaagt aggttctcct gctgctcatt tacttcaata
     2701 gtatctagtt caatgctact aatctttgtt tcttgagcta aggagagttg agaaataaag
     2761 ggagtaatcg ctattgctaa tacagtttgc tttatttgca tattttagat cctctagtta
     2821 gtttaatccg tttaatgtta acactatatc attttaattg aaaattgaaa taataataat
     2881 tattatttaa gtttagataa aaatcaaatg gagtataaaa tgagaacctt acttttttct
     2941 ttcattgccc tatcaatgtg tttaaatacc acaaaggcgg cagaaaaaat tgtttcaact
     3001 gcgggctatg cttccgaaat tgtcgcagca ctaggaaaag ctgataagtt agtcggtgtg
     3061 gataccactt cagttaaacc tcaaacgata atggagaaaa aacctaaaat cggttatcgt
     3121 cgccagcttt ctgctgaagg tattctctct ttaaccccag atttgattat tttagcccct
     3181 gatgccggcc ctaacgcagt tattgagcaa attaaagcca gcggtattcc attacttcac
     3241 ttaggagaga aaccgagctt agcagggatt cgtgaggata ttcagcaaat tgccaaggcg
     3301 attggtgcag aatcagaggc aaaacaatta acagaaacca ttctggcaga tgaaaaaaaa
     3361 ttagccgaca ttcgtagcca acattctact gacaaagaag tcttagtgct gattgacact
     3421 gcatcgcaag gtatctttgg gctagggaaa aagagtgtgg gtgatcattt cttaaccttg
     3481 ttaaatctaa acaatagttt caatgctgag ggcaataagc cactcgccaa tgaggcttta
     3541 gctgcaagca atgccgatgt tattttatta gcctcccgta atcaggcaaa agaatctgca
     3601 acgatcagta aattagcaga atccagccca caatacgccc aactcacaaa tacacaagcg
     3661 ggcaaaaaag gctgtgtttt tgcaatcaat attttagatg ccttgggctt tgggccttac
     3721 actgctcaat atgcacacca aattctcacc actattcaac cttgtttaaa ataacggata
     3781 cgctttgaaa ctgcatttaa ccttaatttt cactttaatt atcagcttag tgatttgtac
     3841 ccatttaggg gcgatgtcat tacccctatc cgcttggcac tgcaacaacg ctgaatgcga
     3901 aagtgcagcc tatattttat ggcaaatccg cctgcctcga ttactagggg cattattcat
     3961 cggggcagcc ctcgctatta gcggagcaac aatgcaaggg ctgtttcgca atccgttagt
     4021 agatccgggg attattggtg tctcttcagg tgctgcattg gctgcggcat tatatattgt
     4081 ggtgattcag ccactactgc cggaatcaat cagctattat gctctaccta ttgccgcctt
     4141 tttagggggc tggctggtga cttttatgct ctatttactt tctcaacgta acgggcaact
     4201 acatatcgcc attatgctat taattggtat tgctctagct gcatttagtg gcgctttaac
     4261 cggcatactg atttatgttg ccgatgataa ccaacttcgt agtttaacct tttggagtat
     4321 gggatcttta gcaggtatga attggcaaat ggtggcaatg atggctgcgg ttgtcttcat
     4381 ttgtagccca cttatttggc gggaacaccg tgcgttaaac gccttaacat taggtgaaga
     4441 agatgcacaa tacattggtt ttgacattgc aaaagtcaaa cgtcgtcttg tgttttacgt
     4501 tgccttatta accggcacaa gcgttgcctg tgttggggct atcggctttg tcgggctggt
     4561 tattccacat ttaatgcgtt tactgggagg cagtaaccac cgccatttat tactcaatag
     4621 tttggtttgc ggtgcaatct tattaatgat tgcagatact cttgccagaa ccctagccgc
     4681 cccggcagaa atcccgattg gtatttttac cgccttattt ggtgcgccat tccttgcagt
     4741 aatggtatgg aaaagtggga aacatatcta atgagcaatt tattaacttg ccaaaattta
     4801 agcatcgttc atcaacaaaa aaagttgctg gagattcaag aaattgcctt tgaacaagga
     4861 aaatttaccg ccatcatcgg tccaaacgga gccggcaaaa caaccttgct caaagcctta
     4921 agcggtaata atgcacaaac taacagtgcc gtattttatc aacatcaacg cttaacagac
     4981 ttaaacggat atgctctggc tcaaaaacgg gcaatgctca gccaacagag ccaaattgcc
     5041 tttcccttaa aagtagaaga attggtacaa ctcggaagag agccatatcg ccaaagtgaa
     5101 tttgcaaaat ttaatgaaaa aataaccgct tgggctatgg aaacaatgtc tttaaccgca
     5161 ttaaaagaaa gaagtagcat acaactttcc ggtggagaac aacatcgagc gcatattgct
     5221 cgagtgctgg ctcaactcgt tcctgaaccg aatgccgatt taagcggaag ctggcttttg
     5281 cttgatgaac ccaccaacca tttggatatt catcaccaat atcagctttt ttccttacta
     5341 caaaagctga aacaacaagg cttaactatc attgcgattt tgcacgatcc tgcattggca
     5401 ataaatcaag ccgatcatat cttaatgcta aaaaatggag aaaaatttgc tgaaatgtcc
     5461 gctaaagcca ttgcagaaag ttgtttatta gatgagctgt atgaaatgaa aatgggctgt
     5521 cgctattgcc ctcacagcgg gcaatattat ctttgccctg cattagctga aagttagggt
     5581 gcgatgcacc ctatcgttta atcttctgca tatccacaag caggcagtag tttaccgtct
     5641 aaatacgctt tgccgtgctg agaaactaat ctacccgaac agaaccactc aatcaccaac
     5701 ggataatatc gatgttcttg ctcaaccact cgagccatta catcttcaat ctcatcttca
     5761 ggataaatcg gcactttagc ttgtaatacc accgcaccag catccacttc ttcattcacg
     5821 aaatgaatag tcgtgccgtg ttcactttcc ccagcctcta tcgctctttg ataagtgttt
     5881 agccctgggt atttaggtaa taaggaaggg tgaatattga gtattttgcc agcaaaatgt
     5941 tgcgtaaatt caggcgttaa aattttcata tagccggcta atacaattaa ctcagctcct
     6001 aactgctcaa tttgttcggc aatcgcaaga tccatttcca cattgctttg gtaatcttta
     6061 cgagaaaata caaatgtcgg gattcccgcc tgtttagctc gaatcagccc ataggcatct
     6121 gccttattgc aaattacacc gcaaatttga ccgcttgtat ccgcagattt ttgcgcatca
     6181 atcattgctt gtaggttaga accattccct gaaattaaaa ccacaaattt tttcatatta
     6241 cacaccggct gaatattcat cgttctggat attatgcctt gaatctcaac atcagtacag
     6301 gaaagaaaaa gggcgtattg gttaaaataa gccactttca agctagaaac gcacctctgc
     6361 ccctagtgtg taagttcgac ctctggcact atttgtgaaa cgataggtgt ctgtatcata
     6421 gtcatactct tctgcggtgg aagagttata cgcatttaag gcatcaagat aatttcggtt
     6481 catcacattt tgtaccccgg cttttaaggt taaatgttta ttcactttgt aatttgcata
     6541 taaatcgata atcaccggaa tattcggtaa ctgctcgtga ataatgttgc catcttcgtc
     6601 taaatcaata ccgacacgtt tcgctttgcc tgtatattta aaaatgctag acagtgttaa
     6661 ttttttatcc agcaatttag cccctagctc caaagtcgca taatcttttg gtagctgggt
     6721 ataagcttca gtgccaaatc cataaagaat agtcgcacct tttgaagtgg gtgctgtgct
     6781 ttcagttcgg gtataactac cattgacaaa aaaagcatcg gaatcgtaag ccagctcaac
     6841 ttcccaacct cgatttttaa ccttttcagg gtgattcaca taaatttgga actgtatagc
     6901 attggtagca tcacttgaat ttcgacatac tgttggccca caaccataca acgagaggtt
     6961 atacaaatag tctttaatgc gtgagttaaa atataccgct tttagcccca agatatcatt
     7021 attgagtaat acattatctt gatgaatatt aaacccaatc tcataggttt tcgcactttc
     7081 tggtttcaaa aacgggttca cactcaagcc atttcgactg gtattaaaca tttcttgcac
     7141 attaggcgaa cgggaggttt gtttgtaact gacaaaaggt tgaaaccaat cagcaacctg
     7201 aaatgctaaa gtggcagaag gattcaactt ataatgtttt ttattaacat caaacccaaa
     7261 agaagggaaa caataatcca tatcatcgct atcgcattct ggcttatatc cttttaactg
     7321 atagcgtgaa tagtgaagac ttgaatttaa agtgacaatg ccgtatttcc attcggtgtc
     7381 taggtaaagg gaatcaatat tttgcttacc tgccggccca aaagcggcat tgctaatcgc
     7441 tccatcatta ttcttacttt ccggtaaatc acgggaatat ttatttccct gactcaaaac
     7501 ggctggtgtt attcaaatca aacatccaca ttttgttttt ggttgaggca tcttctaatg
     7561 tccatagttt tgcatcatcg tggaaatctt gcttagtttc tgttactgct gttaggaatt
     7621 tcagatcaat ccaatcagat tgaggctggt aactggattc taaggaatag gtcacactat
     7681 caatattacg ccccactaag ctatttttat attgacgtcc ggacaattta aactctgaat
     7741 cttgatttgg cttaaaggca atttttccca tataagattc agggtgattt tttaaccctt
     7801 gcatataagc agaatccgtt gccattgtgc cattacctcg gcgataattt tgactgatac
     7861 tacttttgct aaaagccaat aaactgccaa gagaaccact ttccagcaca tcaacttttc
     7921 cggctagtgc aagcattccg ttttttccca gatcattatt gccataatta aaacggctta
     7981 atacgccgac tttatgttgt tctagtagaa tatcttcgac atcaatcgtt ttaaaattag
     8041 cactaccggc taaagcattc aatccggctg caccattgtt gtagcctttc gcaatatcaa
     8101 ctcgggttaa aaaattttga tcaattaaag tgctgaattg gctcattggc ccgttaccat
     8161 cagtgtggaa cttaccacct ccgtttgctg atgtaccaag ctgtgtttgt ggcacgccat
     8221 caactaaggt atttacccga ccaagaccac tcatattacg aatattcaca ctcacgcccc
     8281 cttgccccgg gtcaatttgg gtatagccac ctgcgacaga acggacaata ctatctaaac
     8341 tttgcattgc attcacattc attgctcgag aactcaccga gccggcttta ctgaaattct
     8401 caaaattagg gttttccaca gtgccataaa ccgtcacagt atcaagctcc gcaatatttt
     8461 tcgccttttc ctctgccaat gcagtttgcg atcctcccat acacaaaacc ggaaacaaaa
     8521 tgcgtgtgat tatattaaat ttcataattc tccttattga gtgacccaaa gataatctgc
     8581 ctgtgaagaa gtaatttctt ctctctctgt tttaagcatt agcaccgcta ctttttttgt
     8641 tcggtcatat tccgcaatat gcaacggaac gccaagatct ttgcgaacgt ggtggttacc
     8701 tgcaatcagc aagctagtcg ataacgcatt ttttactaat ttttctgcca ttctgcgatc
     8761 acggaattgt tgtactgcca ccatattttc catcgggtaa ggcattccgt gattcattga
     8821 cagcaacagt tgtgcgatct tttgcttaac ttcagaggaa gtggatacag aaccttttaa
     8881 tggctccgcc ccatttaata aaatttcaac ctctttatct gttaagttag tggctataac
     8941 acgactattt gaacctaagc tctctaccac taaatcttga tacatcttcc aatcccattt
     9001 ttgccattga attgcctctc ttaaatccgt ggctgataaa ttaaccggtc ggttaaactg
     9061 aatgcgatca atcgcaggct gctgatccac atttaacatc tccaacgcaa ttaaatgtaa
     9121 cttggctttc ttcgtaagtt gattaaataa atacaattca atttggtggt gaattaactg
     9181 agtatgctcc tcaccaacta aaacgtaatc ttgagaagat agacgggaaa gtagttcagt
     9241 aaatgaaatc gtttgattgg tattcagatc aataatttgg ccgatagagt ccacgttttg
     9301 ttcattaagg gattgcgttg aaaccgagca gcctaaaaga aaaaaagcca taaaaaggct
     9361 agctaaaaaa gaggaaatac gaggtaacat aacaaaaaat aaattaatga gaattattat
     9421 cattatgtta tgtaaaaaaa gaaaaatcaa tccattttca gaaaacagat aaagaaaatg
     9481 attgataaaa caaaaagcgg taatatttct aaaaaatatt accgcttgaa aaagtgtaaa
     9541 gttgagatta tgcgtacact ggtaaacgtt tacaaatgtc taaaactttc gctttagtct
     9601 ctgcaatcac ctgttcgtga ttatccttac caatgctatc tagcacatcg cacatccagc
     9661 cggctaatgc tgtcacctct gcttctttaa aaccacggcg ggtcacagaa ggcgtaccca
     9721 cacgaatacc tgaagtcacg aatggttttt gagggtcgtt cggcaccgag tttttattca
     9781 cggtaatgtt cgcagctcct aatgcagcat ctgccgcttt gccggttaaa ccttgtttta
     9841 caaagctaac aaggaataaa tggttttccg taccgtttga aaccacatca aaaccacgtt
     9901 gcttaaatac ctcaaccatt gcttttgcat tttttacaac ttgttgctga tatgccttaa
     9961 attccggctc taaagcttct ttaaaacaaa ccgcttttgc cgcaataacg tgcatcaatg
    10021 gtccaccttg accagcaggg aatacggcag attgcagttt cttatacatt tcttcatcgc
    10081 cagatgcaga taaaatcaaa ccacctcgag ggccggctaa ggttttatgg gttgttgtcg
    10141 tcacgacgtg agcgtgagga agcggatttg gataaactcc cgccgcaatt aagcctgcaa
    10201 cgtgagccat atccacaaat aaatatgccc caacttcatc ggcaatctca cgcatttttg
    10261 cccaatccat aacttgagaa taagcagaga aaccagcaac aatcattttc ggtttcactt
    10321 cttgggcttg tttgcgtaat gcttcataat caattaagcc ttcatcagta atgccgtatt
    10381 gttctgcttt ataaatttta ccagagaagc taactgaagc accgtgggtt aaatgcccgc
    10441 cgtgcgctaa actcatacct aaaatagtgt cacccggatt aagcaacgcc atatatacgg
    10501 ctgcattcgc ttgtgagcct gaatgcggtt gaacgttggc ataatctgcc ccgaacaatg
    10561 ctttcgcacg gtcaatcgca agttgttcta caatatccac atactcacaa cccccatagt
    10621 aacgtttacc cggataacct tctgcatatt tattggttaa ttgtgagcct tgagcctgca
    10681 ttacacgtgg gcttgcatag ttttcagatg caattaattc aatatgttct tcttgacggc
    10741 gatcttcatc ttgaatggct tgccataata ctgcatcgta atcagcgata ttcatatcac
    10801 gttttaacat ttttggactc ctaataagct taagtaaacg tttgcgtaag ttgatttcac
    10861 gttat
//
LOCUS       DANLXS010000077        10813 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.077, whole genome shotgun sequence.
ACCESSION   DANLXS010000077 DANLXS010000000
VERSION     DANLXS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.077"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..2411
                     /locus_tag="PXD71_001443"
     CDS             <1..2411
                     /locus_tag="PXD71_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005715618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL6291557.1"
                     /translation="IKSKKLCPTILALSVSSILSSQATYAEETTAELSEVNVVAKEDV
                     KASIEKKNKEIIQQELIQNNRDLVRYSPDVGIVNQGRHQKGFAIRGVEDNRVGISIDG
                     VSPFPDSEENSLYKRYGNLNTSRQSIDPELARTIEVSKGADSFNQGSGNLGGGVNYRT
                     LEPYDIVRNGNKFGAFYRTGYGTRNNEWLNTFGVGYLGEKAEAILVYSNRHGHEMKSA
                     GGYTLPEDSLQTRSLRSSKQTPDDSTHNHHNYLAKFAYRFNDNHRVGVSYSGQNNKNY
                     IIEDSAVTITSFWREAEDRSKRDTVNVFYEYFPESKWISLAKIDADYQDTETSAYNYE
                     GTRAEDATDWSPAKQRKPSDNNIRIFNTELKRLNFRLDSQALEFGKWTHQLSFKASTA
                     ERDFDVLHKDSVYLSGVWVNSPDSTMMYPIKTKQHTFSLHDKIDLTHDWIATLGIRYD
                     WAKYEQQELNGLVCRNCVKTDNAKFNQLTWVTGLEKHLTEAWKVGYNIGTGFRIPNAS
                     EMYFDYRDNAAGAWMSNPNLKAERSLSQNLSLQGSGNVGLLSVNFHHTKYKDFLWEQE
                     TWDVYQAYGREFWRPVQQMQNIDSAKIYGLEVSGKWNLNSAMPIPEGWKLFGAFGYSK
                     GSMSNGADLLSIQPIKAVVGLDYEQPEGKWGVFSRFTFLGAKKAKDAKYLKTLPEKCL
                     KEDRTPNPYYPYWGDEYDIRCTEYSHETGLDTWKHLNAKAFVVDLYGFYKPTENITLR
                     AGIYNLFNRKYHTWDTLRGLNTTGGIVNSVGVRENATYGGYPGLQRYYEPGRNFSANF
                     EYRF"
     gene            2466..2972
                     /gene="hutX"
                     /locus_tag="PXD71_001444"
     CDS             2466..2972
                     /gene="hutX"
                     /locus_tag="PXD71_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDL6291558.1"
                     /translation="MISIEQQTALRNELAENPGQILEMLAAKYQCTLEEVMLNLPAEM
                     LKVTEGERFAEVLQQIHEWDDAITLISHTEDTIIEFVGKLPSGSISRGFYNFEHKEGG
                     GLQGHLRYENCAKIYLLDRPFMGKRTVSLIFINKKGNAMFKIFVGREKIGGALKEHQI
                     KALYQLIG"
     gene            complement(3052..4269)
                     /locus_tag="PXD71_001445"
     CDS             complement(3052..4269)
                     /locus_tag="PXD71_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter"
                     /protein_id="HDL6291559.1"
                     /translation="MKNKILGSALMIAGTTIGAGMLAMPLTSAGMGFGATALLLVGLW
                     ALLAYTGLLFMEVYQTAPQKDVGVASLAEQYFGLIGRVLATFSLLVLLYALLAAYITG
                     GGSLLAGVMPEMADADMKLKISILIFTAVLGTFVVVGVKSVDGLTRVLFLGKIVAFIA
                     VLLMMLPKAKLENLTAIPLDNLLIISAIPIFFTAFGFHVIMGTINSYLDADIGKIRKS
                     IYIGTAIPLVAYLLWQLATHGVLSQNEFTTILTQDPTLNGLVKATSQITGSTILGEMV
                     RLFSVLALITSFLGVSMGIFEGVGDLLKRLNLPTNRLWLTIATFTPPILFALFYPNGF
                     IKALGYAGLLFAFYGMILPIGLAWKARRQYPNLPYRVIGGNLALVVALIAGIIIMVIP
                     FLIQAGYLPQAVG"
     gene            4430..7963
                     /gene="mfd"
                     /locus_tag="PXD71_001446"
     CDS             4430..7963
                     /gene="mfd"
                     /locus_tag="PXD71_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL6291560.1"
                     /translation="MSLISLNLPKSDGNFQDHQTLGNLVGHSDTLAISQAARAFYGLT
                     VVVTPDTRTALRLEKALPQFAGLPVQLFPDWETLPYDNFSPHQDIISARLSALFELQQ
                     GRQQILLLPINTLMQKVCPPNYLANNVLLIKKGDRFSIQKLRLQLENAGYRAVEQVLE
                     YGEYAVRGSILDLFPMGAEEPFRLDFFDDEIDSIRTFDVDNQRTKTEIAEINLLPAHE
                     FPTDSNGIEHFRAKFREKFGEIRREPEHIYQQVSKGILNAGIEYWQPLFFEEMASLFD
                     YFPAQTLFITFADIQQKAEQFHQDTQNRYESHRVDPMRPLLPPSDLWFAIDEVNRWLK
                     GYPRLTITSEKIRKSAAKMNANVANLPDIAIQSSAKEPFSAFQQFRQKFDGNILFSVE
                     SEGRRETLLDLLAPLGVKPKQVNSLDEAQGLPFSLIISPLDQGFVVETGGHGTRPYDD
                     ELNTNGENDNNYVGAGPVPARNLAIICETDLLGERVQTRREKNRKAVNPDTLIRNLAE
                     LKIGQAVVHLENGVGRYGGLTVLDAGGIKAEYLVLHYANDAKLYVPVASLHLISRYIG
                     GADETAPLHKLGSEAWAKTRQKAAEKIRDVAAELLDVYAKRESQTGFAFAYDKAEFDQ
                     FSATFPYEETDDQKIAINAVISDMCQAKAMDRLVCGDVGFGKTEVAIRATFLAVMNHK
                     QVVILAPTTLLAQQHYENFKDRFANYPVNVEVLSRFKTAKEQKTILQNVAEGKVDILV
                     GTHKLLQDDVAFRDLGLLVIDEEHRFGVRQKEKIKQLRANVDILTLTATPIPRTLNMA
                     LNGMRDLSIIASPPARRLLIKTFVRQSDEMVIREAILREILRGGQVYYLHNDVATIEN
                     CAEKLAELVPEARIVIGHGQMRERELERVMSDFYHQRFNLLVCSTIIETGIDVPTANT
                     IIIERADKFGLAQLHQLRGRVGRSHHQAYAYMLTPHPKTLTKDAEQRLEAMSTIDNLG
                     AGFALATHDLEIRGAGELLGSEQSGQIESIGFSLYMDLLENAVKALQEGREPSLDEIT
                     QHQAEIELRVPALLPDDYLPDVNMRLSFYKRIASSENLQSLNDLKVELIDRFGLLPEA
                     TKNLFAITQLRLSAQTLGIKKIDAGIHGGYLEFKPTAQPDPMKFIALIQKQPAVYSFE
                     GAVKFKFRLALEDNRKRLEFVENLLREVVGG"
     gene            8126..8413
                     /locus_tag="PXD71_001447"
     CDS             8126..8413
                     /locus_tag="PXD71_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL6291561.1"
                     /translation="MEEINGFEWDSEKALRNQQKHGVSFIEAVSVFYDNNAMLIPDPD
                     HSSREERFLLLGRSNKDNLLVVVHCERNTNIRIISARLATKRESGQYKGVH"
     gene            8414..8626
                     /locus_tag="PXD71_001448"
     CDS             8414..8626
                     /locus_tag="PXD71_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL6291562.1"
                     /translation="MRDEYDFSNAVKNPYAKGLKSKSVVTIRIEDDVISYFKQLAEEV
                     NLPYQTLINLYLKDCVEQKRKPTISW"
     gene            8742..9002
                     /locus_tag="PXD71_001449"
     CDS             8742..9002
                     /locus_tag="PXD71_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL6291563.1"
                     /translation="MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLR
                     SRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQRDPFEVA"
     gene            9003..9392
                     /locus_tag="PXD71_001450"
     CDS             9003..9392
                     /locus_tag="PXD71_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL6291564.1"
                     /translation="MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYG
                     VAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKLRAEMEKNGKVMGVQDQMIASHA
                     LAEECILVSSDQAFKMVPDLKLENWLH"
     gene            9412..10650
                     /gene="pepT"
                     /locus_tag="PXD71_001451"
     CDS             9412..10650
                     /gene="pepT"
                     /locus_tag="PXD71_001451"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL6291565.1"
                     /translation="MKQSLLNRLLRYTQIHTTSDPKSQTVPSSQQQWDLLHLLKTEVE
                     AMGLSEIYLDPNGYLFASLPANIDKTLPVLGLIAHVDTSPDFNGENVKPQVIEAYQGG
                     NILLNGSGDYLSPTDFPNLNKLIGDTLVTTDGTSLLGADDKSGIAIILSAIEYLQANP
                     HIPHGKIRIGFTTDEEIGRGADHFDVNAFGADVAFTFDGGPLGELQYENFNADAATVT
                     FFGRSVHPGTAKGKMINALERACEFQHRLPPHLTPATSEGREGFIMLHGIEGGIDKAE
                     IHYILRSFERDELAAFGKMLNDTLAEMQQIYGEHCGKVEIVEQYRNMREVLDDYGYLV
                     EIAENAMREQGIEPNLEPIRGGTDGSRLSFMGLPCPNLFTGGENFHGRFEFISVDTME
                     KATKVVIDICRKFADYDFNK"
BASE COUNT         3247 a         2159 c         2472 g         2935 t
ORIGIN      
        1 caataaagtc taaaaaattg tgcccgacta tattggcttt aagcgttagt agcattttgt
       61 cttcacaagc tacctatgcg gaagaaacaa cagcggaatt atctgaagta aatgttgttg
      121 ctaaagaaga tgtgaaggct tctatagaaa agaaaaataa agagataatt cagcaagagt
      181 tgattcaaaa taatcgagat ttagttcgtt atagccctga tgttggcatt gttaaccaag
      241 gtagacatca aaaaggattt gctattcgtg gagtggaaga caaccgtgta ggaataagta
      301 ttgatggtgt ctcgcctttt cctgattcag aagaaaattc cctttataaa cgctatggta
      361 atttaaatac atctcgccaa tctatagatc cggaattagc tcgtactatt gaagtatcta
      421 aaggtgcaga ttctttcaat caaggtagtg gtaatttagg cggcggagtt aattatcgga
      481 cgcttgagcc ttatgatatt gttcgtaacg gtaataaatt tggtgctttt tatcgcacag
      541 gatatggtac tcgtaataat gaatggttga atacattcgg tgttggatat ttaggtgaga
      601 aagctgaggc gatattggtt tattcaaatc gccatgggca tgaaatgaaa agtgcaggag
      661 gttatacact tcctgaagat tctctacaaa ctcgctcttt acgcagtagt aaacaaactc
      721 ctgatgattc tactcataat catcataatt atttagccaa atttgcttat cgatttaatg
      781 ataatcaccg cgttggtgtt tcttacagtg ggcaaaataa taaaaattat attatcgaag
      841 attcagccgt tactattact tctttttggc gtgaagcgga agaccgtagt aaacgtgata
      901 cagtaaatgt tttttatgag tatttcccag agtcaaaatg gatatctcta gcgaagatag
      961 atgcagatta tcaagatact gaaacatctg cttataacta tgaaggtaca cgcgctgaag
     1021 atgcaacaga ctggagccct gctaaacaaa gaaaaccaag tgacaataat attcgtattt
     1081 ttaatactga attgaaacgc ttaaatttcc gtttagacag ccaagcatta gaatttggta
     1141 aatggactca tcagctatct ttcaaagcct ctactgcaga gagagatttc gatgtattac
     1201 ataaggactc ggtttattta tccggtgttt gggtaaattc tcccgattct acgatgatgt
     1261 acccaattaa aacgaaacag cacacttttt cattgcacga taagattgat ttaacgcatg
     1321 attggattgc aacgctaggc attcgttatg attgggcgaa gtatgaacaa caagaactca
     1381 atggtcttgt ttgtcgtaac tgcgtaaaaa ctgacaatgc gaaatttaat caactaactt
     1441 gggttaccgg tttagaaaaa catctgactg aggcatggaa agtaggatat aacattggga
     1501 caggcttccg tattccaaat gcatcagaaa tgtattttga ttaccgtgat aatgctgcag
     1561 gagcttggat gtccaatcca aacctaaaag cggaacgtag tttaagccaa aatttaagtt
     1621 tgcaaggtag tggcaatgtt gggctgctct cagttaattt ccaccatact aaatataaag
     1681 attttttatg ggagcaagaa acctgggatg tttaccaagc atatggtaga gagttttggc
     1741 gcccggtaca gcaaatgcaa aatattgatt ctgcgaaaat ctacgggcta gaagtgagtg
     1801 gtaaatggaa tttaaattca gctatgccaa tacctgaagg ctggaagcta tttggagcct
     1861 tcggttacag caaaggtagt atgtctaacg gggcagattt actttctatt cagccgatta
     1921 aagccgtagt tggcttagat tatgaacaac ctgaaggtaa atggggagta ttttctcgct
     1981 tcacattctt gggggctaaa aaggcgaaag atgcaaaata tttaaaaaca ttaccggaaa
     2041 aatgccttaa agaggataga acgccaaatc cgtattaccc ttattggggg gatgaatatg
     2101 acattcgttg tactgaatat tctcacgaga caggtttaga tacctggaag cacttaaatg
     2161 ctaaagcctt tgttgtggat ttatacggtt tctataaacc aactgagaat attacattac
     2221 gtgccggtat ttataatttg tttaaccgta aataccatac ttgggatact ttacgtggtt
     2281 taaatacaac aggtggaatc gttaattctg ttggagtacg tgaaaatgca acttacggtg
     2341 gctatcctgg attacaacgc tattatgaac ctggacgtaa cttctcggct aattttgaat
     2401 accgtttcta atttacaaat ttacttagag agctaagaaa ctctctttac ttaaaggaac
     2461 atttaatgat atcaatcgaa caacaaaccg ccttacgcaa tgaattagcc gaaaatccgg
     2521 ggcaaatttt agaaatgtta gcggcaaaat atcaatgcac gctggaagag gtaatgctga
     2581 atttaccggc tgaaatgcta aaagtaaccg aaggggaacg ttttgccgaa gtgctacagc
     2641 aaatccacga atgggacgat gcgattacgc tgatttcgca tacggaagat accattattg
     2701 agtttgtggg aaagctccca agcggttcaa tttctcgtgg attttacaat ttcgaacata
     2761 aagagggcgg aggcttacaa gggcatctac gctacgaaaa ttgtgccaaa atttatttgt
     2821 tggatcgtcc ttttatggga aaacgtactg tttcactgat tttcattaat aaaaaaggca
     2881 acgcaatgtt taagattttt gtcggcagag agaaaattgg cggggcatta aaagagcatc
     2941 aaattaaggc gctgtatcag ttgattggct aatctgtaaa aagtcatcga aaaatgaccg
     3001 cttgtaactt atagaaaggg ctacgatatt gaatcgtagc ccttttcaat attagcctac
     3061 cgcttgcggt aaatagccag cctgaattaa aaacggaatg accataatga taataccggc
     3121 aattaacgca accactaatg ccaaattacc accaatcaca cggtaaggta aattcggata
     3181 ttggcgacgt gctttccacg ctaagccgat aggtaaaatc ataccataga acgcaaagag
     3241 taaaccggcg taacctaatg ccttaataaa gccgttcgga tagaataaag cgaacagaat
     3301 tggcggtgta aaggtggcga tggtcagcca taaacggtta gtcggtagat ttaagcgttt
     3361 taacaaatcg cccacgcctt caaaaattcc catcgaaacc cctaaaaatg aggtaatcaa
     3421 tgcaagcact gagaataagc gaaccatttc gcctaaaatc gtactaccgg taatctggct
     3481 agtggctttc accaagccgt ttaaggtcgg atcttgtgtc aaaattgtgg tgaattcatt
     3541 ttggcttaat accccgtgtg tagcgagctg ccacaataaa taagccacta gcggaattgc
     3601 cgtcccgata taaatagatt tacgaatttt gccgatatct gcgtctaaat agctattaat
     3661 tgttcccata ataacgtgga aaccgaacgc agtgaagaaa atgggtatag cagaaataat
     3721 gagtaagtta tctaatggaa ttgctgtcaa gttttccagc ttcgctttag gcagcatcat
     3781 cagcaacacg gcaataaaag caacaatttt tcccaaaaac agtactcggg ttaaaccatc
     3841 aaccgatttc acgccgacta ccacaaatgt acctaatacc gcagtgaaga ttaaaatgct
     3901 gattttcagt ttcatatcag catctgccat ttcaggcatt acaccggcta acagtgaacc
     3961 accaccggta atataagccg caagtagggc ataaagtagt acgagcaaac tgaaagtggc
     4021 taatacacgc ccgattaagc caaagtattg ctcggcaagg cttgccactc caacatcttt
     4081 ttgcggagcg gtttgataaa cttccataaa taataaaccg gtgtaggcca ataaagccca
     4141 taatccgact aagagtaatg cggttgcccc aaaacccatt cctgcagagg tgagtggcat
     4201 tgcaagcatt cctgcaccaa tggtagtgcc tgcaatcatt aatgcacttc ctaaaatttt
     4261 atttttcata atatgtgtga gtaagagtta aaaaagggga acattttatc agaatttttt
     4321 cgcaatgcga tctagataac ataatttctc cgcttatttt gcacaatcag ccgtgcttta
     4381 gtaaactaag caacaatttg caatttttta tttaaaacag accgcttgta tgtctttaat
     4441 ttcacttaat ctgccgaaat ctgacggcaa ttttcaagat catcaaaccc tcggtaacct
     4501 tgtcgggcat tccgataccc ttgcgatcag ccaagctgcc cgagcctttt acggcttgac
     4561 ggtggttgtc acacccgaca cccgcacggc gttacgcttg gaaaaagcct tgcctcagtt
     4621 tgcagggctg ccagtgcaac tgttccccga ttgggaaacc ttgccttatg acaatttttc
     4681 gccgcaccaa gacattatct ccgcccgact ttctgccctg tttgaactgc aacaagggcg
     4741 acagcagatt ttgctgttgc cgatcaacac cttaatgcag aaggtctgcc cgccgaatta
     4801 tctcgctaac aatgtgctgt tgattaaaaa aggcgaccgt ttttcgattc aaaaactgcg
     4861 gttacaactg gaaaatgccg gctatcgggc ggtggaacag gtgttggaat atggtgaata
     4921 cgccgtgcgt ggctcgattt tagacctatt cccgatggga gcagaagagc cgtttcgcct
     4981 cgattttttc gatgatgaga tcgacagtat ccgcactttt gatgtggata accaacgcac
     5041 caaaacggaa attgccgaaa ttaatttact gccggcacac gagttcccga ctgatagcaa
     5101 cgggatcgag cattttagag ccaaattccg tgaaaaattc ggtgaaatcc gccgtgagcc
     5161 ggagcatatt tatcagcaag tcagcaaagg cattttaaat gcagggattg agtattggca
     5221 gccgttgttt tttgaggaaa tggcaagtct gtttgactat ttccctgccc aaacgctgtt
     5281 tatcactttc gccgatattc agcaaaaagc tgaacaattt caccaagata cccaaaaccg
     5341 ctatgaaagc caccgagtgg atccgatgcg accgctactg ccgccgagtg atctctggtt
     5401 tgcgattgat gaggtaaatc gctggctgaa aggctatccc cgtttaacga taacttcgga
     5461 gaaaatccgt aaatcagcag cgaaaatgaa tgcaaatgtg gctaacttgc cggatattgc
     5521 tattcaatcc tctgccaaag agccgttttc tgctttccag caattccgcc aaaaatttga
     5581 cggcaacatt ctgttttcgg tggagagcga agggcgaaga gaaaccttgc tggatttgct
     5641 tgctccgctt ggggtgaagc cgaagcaggt caattcatta gatgaagcac aaggtttgcc
     5701 attttccctg attatttcac cgcttgatca agggtttgtg gtggaaacgg gcgggcacgg
     5761 gacccgtccc tacgatgatg aattaaatac aaatggcgaa aatgataata actacgtagg
     5821 ggcgggtcct gtgcccgccc gcaaccttgc catcatctgc gaaaccgacc tgctgggcga
     5881 acgtgtgcaa acacggcgtg agaaaaaccg caaagccgtc aatcctgata ccttaatccg
     5941 taatttagca gaactcaaaa tcggacaggc ggtggtgcat ttggaaaacg gcgtggggcg
     6001 ttacggtggc ttaaccgtgc tggacgctgg tgggatcaaa gccgaatatc tcgtgctaca
     6061 ttatgctaac gatgccaaac tctatgtgcc ggttgcctcg ctccatttaa tcagccgtta
     6121 tatcggtggg gcggatgaaa ctgcgccgct acataaatta ggcagtgaag cgtgggcgaa
     6181 aacccgccaa aaagcagcgg aaaaaatccg tgatgtggcg gcagaattgc ttgatgttta
     6241 tgccaaacga gaaagccaaa ccggttttgc gtttgcctac gataaagcgg aatttgatca
     6301 gtttagtgcc actttccctt atgaggaaac ggacgatcag aaaattgcca tcaatgccgt
     6361 aattagcgat atgtgccaag ccaaagcgat ggatagattg gtgtgtggcg atgtcggctt
     6421 cggcaaaacc gaagtggcca ttcgagcgac tttcttggca gtgatgaacc ataaacaggt
     6481 ggttattctt gccccaacta ccttgctcgc ccaacagcat tatgagaatt tcaaagaccg
     6541 tttcgccaat taccctgtga atgtggaagt gctgtctcgc tttaaaaccg ccaaagagca
     6601 gaaaacgatt ttgcaaaatg tggcggaggg caaagtggat attttggtcg gcacgcacaa
     6661 actgttgcaa gacgatgtag cgttccgtga tcttgggctt ttagtgattg atgaagaaca
     6721 ccgtttcggc gtacgccaaa aagagaaaat caaacaactg cgtgccaatg tggatattct
     6781 gaccctcacc gccacgccaa ttccaagaac gctgaatatg gcactcaacg gaatgcgtga
     6841 tctctcgatt atcgccagcc cgccggctcg ccgactttta atcaaaacct ttgtgcgaca
     6901 aagtgatgaa atggtaatcc gtgaggcaat tttgcgtgaa attctgcgtg gtgggcaggt
     6961 ttactacctc cataacgatg tggcaaccat tgaaaattgt gccgagaaat tggctgaact
     7021 cgtgccagag gcaagaattg tgatcggaca cgggcagatg cgtgagcgtg aattggagcg
     7081 ggtgatgagc gatttctatc atcaacgctt taacctgctg gtttgctcca ccattattga
     7141 aacggggatt gacgtgccga ctgccaatac cattattatc gaacgggcgg acaaattcgg
     7201 gctggcacaa ctgcaccaac tgcgtggacg ggtcggacgc tcgcaccacc aagcctacgc
     7261 ctatatgctc acaccgcacc cgaaaacgct caccaaagat gccgagcaac gcttggaggc
     7321 gatgagtacc attgataatc tcggggccgg ttttgcgttg gcgacccacg atttagaaat
     7381 tcgtggtgca ggcgaattgc tcggttcgga gcagagcgga cagattgaaa gcatcggttt
     7441 ttcgctctat atggacttgc tcgaaaatgc ggtcaaagct ttgcaagaag ggcgagaacc
     7501 gagcctcgat gaaatcaccc aacaccaagc ggagattgaa ttgcgtgtac cggccttatt
     7561 gccggacgat tatctgcctg acgtgaatat gcgtctgtcg ttctacaagc ggatcgcaag
     7621 ctcggaaaat ctgcaaagcc tgaacgactt gaaagtggag ctgatcgatc gcttcggctt
     7681 gctcccagag gcaaccaaaa acctgtttgc gataacccaa cttcgcttgt cggcacaaac
     7741 gctcgggatt aagaaaatcg atgccggcat tcacggcggt tatcttgaat ttaaaccgac
     7801 agcccagccc gacccaatga agtttatcgc cttaatccaa aaacaaccag cggtgtacag
     7861 ttttgaaggg gcggtgaagt tcaaattccg cttagcactc gaagacaacc gcaagcggtt
     7921 agaatttgtg gaaaatttgc taagggaggt tgtgggcgga taaagcctcc taaggttgtc
     7981 aatcttgaaa aacgattttt gcgatctgta tcgtaaaaat cgtttttgtt tattgcgaat
     8041 ccagtaattt catctaggct gtttataact taaaaaaata gatgtaaata tattttattt
     8101 acaattaaaa aggagtaaac tggcaatgga agaaattaat ggttttgaat gggatagtga
     8161 gaaagctctt cgtaatcaac aaaaacacgg agttagtttt atcgaggcgg taagcgtatt
     8221 ttatgataat aatgcgatgt taatcccgga tccagatcac tcatctaggg aagagcgttt
     8281 tttgctactc gggcgtagca ataaagataa tctacttgtg gttgtgcatt gcgagagaaa
     8341 taccaacatt cggattattt cagcacgttt ggcaacaaaa cgcgaaagtg ggcaatataa
     8401 aggagtacat taaatgagag atgaatacga tttttctaat gcagtgaaaa acccttacgc
     8461 aaaagggcta aaaagcaaaa gtgtggttac tattcggatt gaagacgatg ttatcagcta
     8521 ttttaaacaa cttgccgaag aggtgaattt gccttaccaa acgttgatca atctttattt
     8581 gaaagattgc gttgagcaga aacgtaaacc gactattagt tggtaatatt atgatttgaa
     8641 atcagtatgt tattgagtat ttgagaatgg gttatcttgc cagttgtata aaataggcat
     8701 atactttggc atatacacta tctatcggag ggtaaagtaa gatggaaaaa attgcgagat
     8761 tattccgtaa cgggcggaac caagcagtgc gtttaccggt tgaatttgag tttgatgcag
     8821 agcaagtcta tgtttctaaa aaagaaaatg gcgatatttt attatcattg agatcgagaa
     8881 ataccgagca ttggcaacga ttgtttgagc tattgcctgc tgttgtatgt gatgaaacat
     8941 ttttagatac aaaagagaga aaccaatcct ttgagcaacg ggatcctttt gaggtagctt
     9001 aaatgtatat gttagatacg aatactgtaa gctatttttt tcgtggaaat gcaaatgtgg
     9061 tggcgaaatt gaagtccatt aatcctgaga ggctttgtat ttcatctgta actgctgctg
     9121 aattggttta tggtgtggcg aaaagaaata atgggcagct ttctcaattt ttagattatt
     9181 ttttatcgag tgtaaaggtt ttagattgga attatcgttg tgctgaactt tatggaaagt
     9241 tacgggcaga gatggagaaa aatggcaaag taatgggcgt gcaggatcaa atgattgcta
     9301 gtcacgctct tgctgaagag tgtattttag tttctagtga tcaggcattt aaaatggtac
     9361 ctgatctgaa attggagaat tggttacact aaactatagt aaggaaaaac aatgaaacaa
     9421 tcccttctta accgtttact acgttacacc caaattcaca caacttcaga tccgaaaagc
     9481 caaactgtgc caagctcgca acagcagtgg gatttgcttc atctgctgaa aacggaagta
     9541 gaggcgatgg ggctaagcga gatttatctt gaccccaacg gctatctgtt cgccagcttg
     9601 cctgccaata ttgacaaaac cctgccggtg cttgggctga ttgcccacgt tgatacttcg
     9661 cccgatttta atggtgaaaa cgtgaaacca caggtgattg aggcttatca aggcggcaat
     9721 attttgctaa acggcagtgg cgattatctt tcgccaacgg atttcccgaa tttaaacaag
     9781 ctgattggcg acaccttagt caccactgac ggcacctctt tgcttggcgc agacgacaaa
     9841 tccggcattg cgattattct ctcggctatc gagtatttac aagccaatcc gcatattccg
     9901 cacggcaaga tccgcatcgg ctttaccacc gatgaagaga tcggtcgagg cgcagatcat
     9961 tttgatgtaa acgcattcgg tgccgatgtc gcctttacct ttgatggcgg gcctctgggc
    10021 gaattgcaat atgaaaattt caatgccgat gcagcgaccg ttacgttctt cgggcgtagc
    10081 gttcaccccg gcacggcaaa gggcaagatg atcaacgcct tagagcgagc ttgcgagttc
    10141 caacaccgtc tgcctcccca tttaacccct gccaccagcg aagggcgtga aggctttatt
    10201 atgttgcacg gcattgaggg tggaattgat aaagcggaaa ttcactatat tttacgtagc
    10261 tttgagcgtg atgaattagc tgcattcggc aaaatgctga atgatacgct ggcagagatg
    10321 caacagattt acggcgaaca ttgcggaaaa gtcgagattg tcgagcaata tcgcaatatg
    10381 cgtgaagtgc tggacgatta cggctacttg gttgaaattg ccgaaaacgc aatgcgtgaa
    10441 caaggcattg agccgaattt agaaccgatc cgtggcggta ctgatggctc acgcctttca
    10501 tttatgggct tgccttgccc gaatttattt accggcggcg aaaatttcca cggacgattt
    10561 gagtttattt cggttgatac gatggaaaaa gccacaaaag ttgtaattga tatttgtcgg
    10621 aaatttgctg attatgattt caacaaataa ggcggctgca agcggtcaaa tttgatgaaa
    10681 aatttgcaaa aagcgttatt tttcaccaat taaacgattt tttacagatt tcctacaaat
    10741 ttatttgaca ttaaatctga aatccataga atatgcacct gttgagactc tacgaggttc
    10801 gtagcgtttt taa
//
LOCUS       DANLXS010000078        10724 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.078, whole genome shotgun sequence.
ACCESSION   DANLXS010000078 DANLXS010000000
VERSION     DANLXS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.078"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..860)
                     /gene="pdxY"
                     /locus_tag="PXD71_001452"
     CDS             complement(<1..860)
                     /gene="pdxY"
                     /locus_tag="PXD71_001452"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDL6291566.1"
                     /translation="MKNILSIQSHVVYGYAGNKASTFPMQLLGVDVWALNTVQFSNHT
                     QYGKWGGMVMPKEQIGSIVDGIHNIGELHRCDAVLSGYIGSAEQVAEIIQAFHKVKAE
                     NPNAIYVCDPVMGHPDKGCIVADGVKEGLINLAMAQADIITPNLVELRELTGLTVENF
                     EQAVEAVKVILTKGPKKVLVKHLSRVGKESNKFEMLFANQDGIWHISRPLYQFAKEPV
                     GVGDLTAGLFVANLLNGKSDLEAFEHTANAVNEVMEQTARSGLYELQIIASRDRIVNP
                     QCSYQAVKIA"
     gene            979..1054
                     /locus_tag="PXD71_001453"
     tRNA            979..1054
                     /locus_tag="PXD71_001453"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1012..1014,aa:Thr,seq:tgt)
     gene            complement(1149..2102)
                     /gene="accA"
                     /locus_tag="PXD71_001454"
     CDS             complement(1149..2102)
                     /gene="accA"
                     /locus_tag="PXD71_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL6291567.1"
                     /translation="MSQEFLDFELPIAELEAKIESLRAVSEQDDKINLDDEIKRLQKK
                     SEELTKKTFANLDAWQISRMARHPNRPYTLDYIEHIFTEFDELAGDRAFADDKAIVGG
                     IARLDGRPVMVIGHQKGRTVKEKVKRNFGMPAPEGYRKALRLMEMAERFNMPIITFID
                     TPGAYPGIGAEERGQSEAIARNLREMSQLSVPVICTVIGEGGSGGALAIGVGDKVNML
                     QYSTYSVISPEGCASILWKSADKASTAAEVMGLTAPRLKELELIDNIVPEPLGGAHRN
                     VEQIAQNLKQRIVEDLAGLDQLNKEDLLERRYQRLMNYGYV"
     gene            complement(2156..3580)
                     /locus_tag="PXD71_001455"
     CDS             complement(2156..3580)
                     /locus_tag="PXD71_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDL6291568.1"
                     /translation="MKKQITRRQFLQGSALAAGFATVPTPLLAAERPQLNIPPLMDIG
                     RGKPVRLDLRPAQTQFDKGKLVDVWGVNGQYLAPTVRVKSGDFVKLTYLNNLPQKISM
                     NIQGLQASSEMIGTVHRSLDPKSSWSPIVSINQPACTCWYHADTMLNSAFQVYRGLAG
                     LWMIEDEQSKKATLPNKYGVNDIPLILQDQLLNKDGVQVLDKNTSQFFGKRLFVNGQE
                     SPYFNVPRGWVRLRIVNASLSRAYELRLDNEQPLYLIATGLGMLAEPLETESVQLAPS
                     ERVEVLVDLSEGKAVSLISGQKRDIFYKIGQVFADNDELNDNVILELRPEGMAAVLNN
                     KPSLPAFNLEEFNLKISQERRFAIRPADYLINQKRFDPKRIDFTAQKGSVERWYLHST
                     QAVGFTLQGAKFIIETLNRKQYPIKQLAWRDTVWLEAEQEITLLVRFDNTAPEQLPFT
                     FGVSDFMLRDRGTMGQFSVIENGQ"
     gene            complement(3582..4316)
                     /locus_tag="PXD71_001456"
     CDS             complement(3582..4316)
                     /locus_tag="PXD71_001456"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDL6291569.1"
                     /translation="MLKIFRILIVALAAIFISIFGTIYALLRLRHPSSVGVVARWFGA
                     LHKLVGLELITRKKPEINYPVVYIGNHQNNYDMVTISSMVAEKTVSIGKKSLIWIPFF
                     GLVYWATGNIFIHREKRSSAIETMNKAGQIVKERNVSVWMFPEGTRSRGRGLLPFKTG
                     AFHLAISAGVPIVPVVCSNLHNKVDLNRSNNGTVICEMLDPIDTSGYSRENVKELMEK
                     CHQIMLAKFEQLNQEVEQLENTAKNA"
     gene            4463..4960
                     /gene="tpx"
                     /locus_tag="PXD71_001457"
     CDS             4463..4960
                     /gene="tpx"
                     /locus_tag="PXD71_001457"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDL6291570.1"
                     /translation="MSKVTLAGSPIDVAGTFPQKGDVVADFTLVNSDLSDVSLSDFDG
                     KRKVLNIFPSIDTGICATSVRVFNQKAADVANTVVLCISADLPFAQARFCGAEGIENA
                     KVLSSFRHRDLHNKLGVDIISGPLAGLTSRSVIVLDENNKVLHSELVPEIKQEPDYEA
                     ALAVL"
     gene            complement(5045..6670)
                     /gene="yidC"
                     /locus_tag="PXD71_001458"
     CDS             complement(5045..6670)
                     /gene="yidC"
                     /locus_tag="PXD71_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL6291571.1"
                     /translation="MNNRGLLLMGLFLVSILIFTQWQQDFDPEIQAQKQAQVAAQTQQ
                     AGDLPSASNANNPIADSITQGKTITVESDVLRLTIDTLGGDVIGSDLLKHNAELHSNT
                     PFKLLQQDGDKIYVAQSGLVGKNGIDTNAGRPQYAVTADSFKLAEGQNEISVPLTFEK
                     EGVVYTKTFTLKRGSYDVGVNFNIKNNTAQAIEVQPYGQLKHSIVESSGNLTMPTYTG
                     GAYSSSETNYKKYSFQDMEKANLSVDTKAGWIAVLQHYFVSAWVPNQDADNSLYSRTN
                     NGVATIGYRGPLTTIAPNSEANLNSKLWTGSKDQKEMGESANHLDLTVDYGWAWFIAK
                     PLFWLLTAIQKIVSNWGLAIIGVTIVVKTILYPLTKAQYTSMAKMRMLQPRITEMRER
                     FGDDRQRMSQEMMKLYREEKVNPMGGCLPILIQMPIFIALYWTFMEAVELRHAPFFGW
                     IQDLSAQDPYYILPLLMGGSMFLLQKMSPTPVADPVQQKVMTFMPVIFTVFFLWFPSG
                     LVLYWLTSNLITIAQQWLIYRNLEKKGLHSRKK"
     gene            complement(6823..7248)
                     /locus_tag="PXD71_001459"
     CDS             complement(6823..7248)
                     /locus_tag="PXD71_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HDL6291572.1"
                     /translation="MTIWKQTATCEQLNTLSQKSAVSHLGIEFTAIGEDWIEAQLQVD
                     ERTQQPFGVLHGGVSAALAETTANAGALMVCEPHQIAVGMELNISHLKSIPAGTKAIA
                     KATPLKLGREVQVWNIDIKDEQGNLCAVARLSTKTLDKR"
     gene            complement(7577..10669)
                     /locus_tag="PXD71_001460"
     CDS             complement(7577..10669)
                     /locus_tag="PXD71_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL6291573.1"
                     /translation="MIPRLSEVPQLDKATQSYLTELVNQHFSGEIASNYADRLSLATD
                     NSVYQQIPQAILFPKSVSDVVILTKLAQKPEYQHLTFTPRGGGTGTNGQSLNNNIIVD
                     LSRHMNQILELNVEERWVRVQAGVVKDQLNQFLKPHGLFFSPELSTSNRATLGGMINT
                     DASGQGSLQYGKTSDHILAIKSVLINGDILETQAVKSADFFAHIEGLNLSALGKTLHR
                     EIFNRCKTLRPKVLNELPQLNRFITGYDLKNVFNHGESEFNLTRILTGSEGSLAFICE
                     AKLNLLPIPKYRTLINIKYNSFDAALRSAPFMLEAKALSVETVDSKVLNLAKQDIVWH
                     SVSDLLTEDPANPILGINIVEYASSDQTQIETQVKALCQSLDQKMANNEAGIIGYQLT
                     SDVNSIEKIYAMRKKAVGLLGNAKGWAKPIAFVEDTAVPPENLADYIVEFRALLDRYN
                     LPYGMFGHVDAGVLHVRPALDLCDKAQVETFKQISDQVVELTAKYGGLIWGEHGKGMR
                     SYYGERFFGETLWQELRYIKTLFDPYNRLNPGKICTPLESETSLYPIDSQMRADFDRQ
                     IPIQVREEFKGAMNCNGNGLCFNFDVHSTMCPSMKVSGNRLYSPKGRATLVREWLRLL
                     TEQNVSPDDLIFKPRKQAVKLADFVAKIRNTLNKNKEYDFSHEVKAAMDTCLACKACA
                     SQCPIKIDVPTFRSQFNELYHSRYLRPLKDYVVSNLEFIAPLMAKQPRFFNFFSTSTF
                     AEKAANKTIGMTYLPALSVPNLQHQLVEVGYQGESLESLEQQATSGDFSAKNCKTLFI
                     VQDPFTSYYDAKVVADFIALIKKLGFKPIVLPFKPSGKAQHVKGFLHKFAKTAKNQAD
                     FLNRVAKLGLPMVAVDPALTLLYREEYKEILKEARGDFKVLLAHEWLREVIKNGELEN
                     CKKSANSDRLPWYLFAHCTESTALPSSMKEWQAIFTHFGETLLNENVGCCGMAGTFGH
                     ETKHVEMSKAIYQQSWQIKLKNKPLERCLATGYSCRSQVKRFEQQGIKHPIQALLDVI
                     "
BASE COUNT         2856 a         2292 c         2300 g         3276 t
ORIGIN      
        1 taggcaatct ttacagcttg gtaactacac tgtggattaa caatccgatc acgagaagca
       61 ataatttgta actcataaag ccctgatcta gcggtttgtt ccattacctc attgaccgca
      121 tttgcagtgt gttcaaaggc ttctagatcg gattttccat ttaataagtt cgccacaaat
      181 agccctgcgg ttaaatcgcc tacaccgaca ggttctttgg caaattggta gagtgggcgg
      241 ctgatgtgcc aaatgccatc ttggttggcg aacagcattt caaatttgtt gctctctttg
      301 ccgactcggc ttaaatgttt aaccaatact tttttcgggc ctttggttaa aatcactttc
      361 actgcctcga ccgcttgctc aaaattttcc accgttaaac cggttaattc tcttaattcc
      421 actaaatttg gtgtgataat gtcggcttgt gccattgcca gattgatcaa tccctctttt
      481 acgccgtctg ccacaatgca gcctttgtcg gggtgtccca ttaccgggtc gcatacataa
      541 attgcgtttg gattttccgc tttcacttta tgaaaagcct gaataatttc ggctacttgt
      601 tctgccgagc cgatatagcc agagagtacg gcatcgcaac gatgcagttc accgatattg
      661 tgaatgccat caacaatgct accgatttgt tcttttggca tcaccatacc gccccatttt
      721 ccatattggg tatggttgga aaactgtacg gtatttaacg cccatacatc aacacctaac
      781 agctgcatag gaaaagttga agccttattg ccggcatagc cgtagacaac gtgcgattgt
      841 atggatagta tgtttttcat tcattcactc ctttaaaatt tcaatatgtg ttgtttttta
      901 gtcatttgaa gttgcaagga cttgaagctc atcaagaaat ctctataatc ccgcctcgtt
      961 ctcttatcca ttcaatatgc cgacttagct cagtaggtag agcaactgac ttgtaatcag
     1021 taggtcacca gttcgattcc ggtagtcggc accatcacct ttattttctg atgagtcccc
     1081 tttaatgtaa aatggcaaga ttttgcaaat tttcacaaaa atcttaccgc ttgtagggtg
     1141 aaattgaatt agacgtaacc gtaattcatt aagcgttgat aacggcgttc cagtaaatcc
     1201 tctttattaa gttgatctaa ccctgccaaa tcttcaacaa tacgctgttt aaggttttgg
     1261 gcgatttgtt caacattgcg atgagcaccg cctaagggtt ccggcacgat gttatcaatc
     1321 agctcaagct cttttaaacg aggggcagtt aaacccatta cttctgctgc cgttgaggct
     1381 ttatcggcac ttttccacag aatcgaggca cagccttcag gtgaaattac cgagtaagtt
     1441 gaatattgca acatattgac cttatcgccc acaccgatcg ctaatgcacc gcccgagcca
     1501 ccttcgccaa ttaccgtaca gattactggc acggaaagtt gagacatttc acgcaggtta
     1561 cgggcaatcg cttctgactg accacgctct tccgcaccaa tgcccggata agctcccggt
     1621 gtgtcgataa aggtgatgat tggcatatta aagcgttctg ccatttccat taaacgtaac
     1681 gctttgcgat agccttcagg agcaggcata ccaaagttac gtttcacttt ctcttttacg
     1741 gtacgacctt tctgatgacc gataaccatc accggacgtc cgtccagacg ggcaatacca
     1801 ccgacaatcg ctttatcatc ggcaaaggca cggtcgcctg ccagttcgtc aaattcggtg
     1861 aaaatatgct caatataatc taaagtgtac gggcgattgg ggtggcgtgc catacgggaa
     1921 atttgccacg catctaaatt agcaaaggtt ttcttggtta attcctcgct tttcttttgt
     1981 aaacgcttga tttcatcatc aagattaatt ttgtcatctt gctcgctgac tgctcttaaa
     2041 gactcgatct tggcttccaa ttcagcaatc ggcaattcaa aatctaaaaa ttcttggctc
     2101 attctatttt cctttttatg ggcaaaatta tggggcatta tcgcttatca gtcggttatt
     2161 gtccattttc aattacgcta aattgaccca tcgtacctct atcacgcagc ataaaatcgc
     2221 tcacgccaaa ggtaaatggc agttgctcag gggcagtatt atcaaagcga accaacagcg
     2281 tgatttcttg ctccgcctcg agccaaacgg tgtcccgcca cgcaagttgt ttgatagggt
     2341 attgcttgcg gtttagggtt tcaataatga attttgcacc ttgtagcgta aatccgaccg
     2401 cttgtgtgct atgtaaatac caacgctcaa cgctgccttt ttgcgcggta aaatcaatgc
     2461 gttttgggtc aaaccgcttt tggttgatta aataatcagc cggacgaatg gcaaaacgtc
     2521 tctcttggct gattttcagg ttaaattcct ctaagttgaa agccggtaaa ctcggtttat
     2581 tattcaatac cgctgccatt ccttcagggc gaagctctaa aatcacgtta tcattcaatt
     2641 cgtcgttgtc agcaaagacc tgcccgattt tgtaaaaaat atctcttttt tgaccgctta
     2701 tcagcgatac cgctttccct tcgcttaaat cgaccaacac ttccaccctt tcactcggtg
     2761 caagttgaac cgattcggtt tcaagtggct ctgccagcat tcctaagccg gtggcgatta
     2821 ggtacaacgg ctgctcgtta tctaagcgta attcataagc acgggaaagt gaggcattga
     2881 cgatccgcaa tctgacccag ccacgaggca cgttaaaata aggcgattcc tgcccgttga
     2941 caaataatcg cttaccgaaa aattgtgagg tgtttttatc taacacctgt acgccgtctt
     3001 tgttgagcag ctggtcttgc agaattaacg gaatgtcgtt cacgccatat ttattcggta
     3061 aagtggcttt tttgctctgt tcatcttcaa tcatccacaa gccggctaag ccacgataca
     3121 cttggaatgc cgagttgagc attgtatcgg cgtgatacca acaagtacaa gccggttgat
     3181 tgatagaaac aatcggcgac cagctacttt tggggtcaag ggaacgatgc accgtgccaa
     3241 tcatctcact tgaggcttgc aatccttgaa tattcattga gattttttgc ggtaaattat
     3301 tgagataagt taatttgaca aaatcccccg attttacccg tacggttggg gcaagatatt
     3361 gcccgttcac accccataca tcaaccaatt tgcctttatc aaattgggtt tgtgcggggc
     3421 gtaaatccaa ccgaaccggc tttcctcgcc caatatccat taaaggcgga atgttcagtt
     3481 gcggtcgctc tgctgctaat agcggtgttg gcactgttgc aaatccagcc gcaagggcag
     3541 agccttgtaa aaattggcgt cgggtgatct gttttttcat attaggcgtt ttttgcagtg
     3601 ttttctaact gttcgacttc ttgattaagt tgttcaaatt ttgccaacat aatttgatgg
     3661 cacttttcca tcagttcttt gacgttttca cgggaatagc ctgaagtatc aatcggatca
     3721 agcatttcac agattactgt gccgttattt gaacggttca aatccacttt attgtgtaaa
     3781 ttagagcaaa ctaccggcac aatcggcacg ccggcagaaa tggctaaatg gaatgcaccg
     3841 gttttaaatg gcagtaaacc acgcccacgg ctacgagtac cttccgggaa catccacacc
     3901 gacacattgc gttctttcac gatttgccct gctttattca ttgtttcaat cgcactggaa
     3961 cgcttttcac ggtgaataaa aatattgccg gttgcccagt aaaccaaacc gaaaaacgga
     4021 atccaaatca ggcttttttt gccgatactc accgtctttt ctgccaccat tgaggaaatc
     4081 gttaccatat cgtaattgtt ttgatgattg ccgatataga caaccggata gttaatttcc
     4141 ggctttttgc gggtaattaa ttccaacccg actaatttat gcaaagcacc aaaccaacga
     4201 gctaccacac ccacgctact tgggtgacgt aaacgcagta acgcataaat agtgccaaaa
     4261 atagaaatga aaatagcggc aagggcaaca attaaaatac gaaaaatttt taacataatg
     4321 gaataacctt aaaaataaat tgtctaggag tatagcaaaa taaggttatt ttctaaaggc
     4381 tatccacatt aaattgatag tttcctattt tgcaaaaaat cggtatattt aaaccgcttg
     4441 tttatttgat aaggagaaaa aaatgagtaa agtgacttta gcaggaagcc cgattgatgt
     4501 tgcaggcaca tttccacaaa aaggcgatgt ggttgccgat tttaccttag tcaacagtga
     4561 tttaagtgat gtttcgcttt ccgattttga tggcaaacgc aaagtgttaa atattttccc
     4621 aagtattgac accggcattt gtgccacttc tgtgcgtgtg tttaatcaaa aagcggctga
     4681 tgtagcgaat acggttgtcc tttgtatttc agcggattta ccttttgccc aagcacgttt
     4741 ctgtggagca gaaggaattg aaaatgccaa agtattatcc agtttccgtc atcgtgattt
     4801 acacaacaaa ctgggggtag atattatttc aggtccgttg gcaggcttaa cttcacgttc
     4861 agtaattgtg ttagatgaaa acaacaaggt attacacagc gaattagtgc cggaaattaa
     4921 acaagagccg gattatgagg cggcgttagc ggtattataa tttgcagaat tatgctggaa
     4981 aataaccgct tgtaaagcaa caaaaagccc gttgattcaa ttcaacgggc tttgttttta
     5041 gtgattattt cttacgagag tgtaagcctt ttttctcaag gttacggtaa atcaaccatt
     5101 gctgtgcaat cgtgattaag tttgaggtta accagtaaag cactaagcct gatgggaacc
     5161 ataagaagaa cacggtaaag ataaccggca taaaggtcat tactttttgt tgtaccgggt
     5221 cagctaccgg tgttggcgac attttttgta acaagaacat cgaaccaccc attaataaag
     5281 gcaaaatgta gtatgggtct tgtgccgata agtcttgaat ccagccgaag aaaggtgcgt
     5341 ggcgtaattc taccgcttcc ataaatgtcc aatataaagc aatgaaaatc ggcatttgga
     5401 ttaaaattgg taagcagcca cccattgggt tcactttttc ttctctgtag agtttcatca
     5461 tctcttggct catacgttgg cggtcgtcgc cgaaacgttc acgcatttca gtaatgcgag
     5521 gttgtaacat acgcattttt gccatcgagg tgtattgtgc tttggttaat gggtaaagaa
     5581 tggttttcac tacaatagtg acaccgataa ttgccaagcc ccagttgctt acgatttttt
     5641 gaattgcggt tagtaaccag aaaagcggtt tagcgataaa ccacgcccag ccgtaatcta
     5701 cggttaagtc taggtgattt gctgattcgc ccatttcttt ctgatcttta gaacctgtcc
     5761 aaagtttgct atttaaatta gcctctgaat ttggcgcaat cgtagttaaa gggccacggt
     5821 aaccgatagt tgctacccca ttattggtgc gtgaataaag gctgttatcc gcatcttggt
     5881 ttggcaccca agcagacacg aaatagtgct gtaataccgc aatccaaccc gctttggtgt
     5941 ccacgcttaa gttggctttt tccatatctt ggaaactgta ttttttgtag ttggtttctg
     6001 aagaagaata agccccgccg gtgtaagtcg gcatggttaa attgccggaa ctttcaacta
     6061 tagagtgttt taattgcccg taaggctgaa cttcaatcgc ttgtgcagta ttatttttga
     6121 tattgaagtt taccccaaca tcgtagctac cacgttttag cgtgaaagtt ttggtgtaaa
     6181 ccacaccctc tttttcaaag gttaatggta cagaaatttc attttgccct tcggctaatt
     6241 tgaagctatc tgccgttacc gcatattgtg ggcgaccggc attggtatca atcccgtttt
     6301 tacccactaa accgctttga gcaacgtaga ttttgtcgcc gtcttgctgt aatagtttaa
     6361 atggcgtgtt agagtgtaat tcagcattgt gttttaacaa atctgagccg atgacatcac
     6421 cgcctaaggt atcaatagtc agacgtaata catcgctttc cacggtaatg gttttacctt
     6481 gcgtgatgct atctgcgatt ggattattgg cattggaagc agacggaaga tcacccgctt
     6541 gttgggtttg tgctgccact tgagcttgtt tttgtgcttg aatttcagga tcaaaatctt
     6601 gttgccattg tgtaaaaatc aaaatagaca caagaaataa acccattagc agtaagccac
     6661 gattattcat gttggaattc tcttgttagt aatggataaa aagaaaggta ttttataata
     6721 ggttagcttg ttttccaaac gccttttatg aacaagcggt cggattttcc aagttttttg
     6781 caaaattttc ggagaatttg accgcttgtg gagaataaga aactagcgtt tatctaaggt
     6841 tttggtggaa aggcgagcaa ctgcacacag gttaccttgt tcgtctttaa tgtcgatatt
     6901 ccaaacctgt acttcacgcc ctaatttcag cggtgtagct ttggcaatcg cttttgtgcc
     6961 tgccggaatc gattttaaat ggctgatgtt tagctccatt cccaccgcaa tttgatgggg
     7021 ttcacacacc attaacgccc ctgcattggc agtagtttcc gcgagtgctg ccgatacacc
     7081 gccgtgcaat acgccaaacg gctgttgagt gcgttcgtcc acctgaagct gtgcttcaat
     7141 ccaatcttca ccgatagcgg taaattcaat ccctaagtgt gacaccgccg atttttggct
     7201 gagtgtgttt aattgttcac aagttgctgt ttgcttccaa atggtcatta ttatttcctg
     7261 tctaataaaa aagctgtcta tttagacagc tttcattatc tctttgagcg aaacatacta
     7321 cgccaatagt acattgtttg gtcgcccata ttaccttaag ccatatagca ctaaggaggt
     7381 gatttatgtg agaaaatcag aagttttctt tctggttgcg ttgctaatat tattagcaac
     7441 ttccgctagc attaaagtac tcaccatact tgatgtgcta gtttggttat tcgctagata
     7501 acctaaaatc agacagaggc aggctggtta cctgcctcgc tttttattct aagtactttt
     7561 aggttacatt tcaaccttaa atcacgtcca gcaacgcttg aattgggtgt ttaatgcctt
     7621 gttgttcaaa acgtttgact tgcgagcggc aggagtagcc ggttgctagg caacgctcaa
     7681 gcggtttatt ttttaattta atttgccatg attgctgata aatcgctttc gacatttcaa
     7741 cgtgtttggt ttcgtgtccg aacgtgcctg ccattccaca gcaaccgaca ttttcattga
     7801 gtaaggtttc accaaaatgg gtgaaaatag cttgccactc tttcattgag cttggcaagg
     7861 cggtggattc ggtgcagtga gcaaataaat accacggcaa gcggtcggaa tttgccgatt
     7921 ttttgcaatt ttccagctcg ccgtttttaa tcacttccct taaccattcg tgtgccaata
     7981 agactttgaa atccccccga gcttctttga ggatctcttt gtattcttca cgataaagca
     8041 aagttaaggc aggatcgaca gccaccatcg gtagccccaa tttcgccaca cggtttaaaa
     8101 aatcggcttg gtttttcgct gttttcgcaa atttatgcaa aaagccttta acgtgttgtg
     8161 ctttaccgct tggcttgaat ggcaacacaa tcggcttaaa gcctaatttt ttaatgagag
     8221 cgataaagtc tgccaccact ttggcatcgt aataagaggt aaacggatct tgcacaataa
     8281 acaaggtttt gcaatttttt gcagaaaaat caccgcttgt agcttgttgt tcaaggcttt
     8341 ctaagctctc gccttggtag ccgacttcca ccaactgatg ttgcagattt ggcactgaca
     8401 atgccggcaa ataggtcatt ccaatggttt tgtttgcagc tttttcggca aaagttgagg
     8461 tgctgaaaaa gttaaaaaaa cgtggttgtt tcgccattaa aggggcgata aattctaagt
     8521 ttgagaccac ataatctttc agtggtcgca aatagcggct gtggtaaagc tcgttaaatt
     8581 gcgatctgaa cgtcggtacg tcaattttga ttgggcattg gctggcacag gctttgcagg
     8641 caaggcaggt gtccatcgcc gctttgactt cgtgtgaaaa atcatactct ttgtttttat
     8701 tgagcgtgtt gcgaattttt gcaacaaaat cagctaattt gaccgcttgt ttgcgaggtt
     8761 tgaagatcaa atcgtccggc gaaacgtttt gttcggtaag taatctgagc cattcacgca
     8821 caagagtagc ccgccctttc ggtgagtaaa gacggttgcc cgagactttc attgaagggc
     8881 acatcgtgct gtgtacatca aagttaaaac atagcccgtt gccgttacaa ttcattgctc
     8941 ctttaaattc ttctcgcact tggattggaa tttggcggtc aaaatcggct cgcatttgtg
     9001 agtcaatcgg gtagagcgag gtttcgcttt ctaatggtgt acaaatttta cctgggttca
     9061 agcgattgta cgggtcaaat aaggttttga tgtaacggag ttcttgccac aaagtttcgc
     9121 caaagaaacg ttcgccgtag taagaacgca tacctttgcc gtgttcgccc caaatcaagc
     9181 caccgtattt ggcggtgagt tcgactactt ggtcggaaat ttgcttaaag gtttccactt
     9241 gggctttgtc gcataagtca agagcagggc gaacgtgtag cacgccggca tcaacgtggc
     9301 cgaacattcc gtaaggcaga ttatagcgat ctaataaagc tcgaaattcc acgatgtaat
     9361 cggcaaggtt ttccggtggc acagcggtgt cttctacaaa ggcgatcggt tttgcccagc
     9421 ctttggcatt gcctagcagg cctacggctt ttttccgcat tgcgtagatt ttctcgatag
     9481 aatttacatc actcgtgagt tggtagccga taatcccggc ttcattattt gccatttttt
     9541 gatcaaggct ttggcagagt gctttcactt gggtttcaat ttgagtctga tcgcttgagg
     9601 catattcaac aatgttaatc cctaaaatcg ggttggctgg gtcttcggtt aataaatctg
     9661 acaccgaatg ccagacgata tcttgcttgg caagattgag cactttggaa tcaaccgttt
     9721 ccaccgataa ggcttttgcc tctaacataa aaggagcgga gcgtaaggcg gcatcaaagg
     9781 aattatattt aatattaatt aaggtgcgat atttgggaat cggcagtaaa ttcagtttgg
     9841 cttcgcaaat aaaggcaagc gaaccttcag aaccggttaa aatacgggta aggttaaatt
     9901 cgctttcacc atggttaaac acatttttta aatcatagcc ggtaataaaa cggttaagct
     9961 gaggcagttc gttcagcact ttggggcgta atgttttgca acggttgaaa atttcacggt
    10021 gcaaggtttt gccgagagcg gagagattta acccttcaat atgtgcaaaa aaatcggcag
    10081 atttgaccgc ttgtgtctct aaaatatcgc cgttaataag cacagatttg atggcaagaa
    10141 tatgatcaga tgttttgcca tattgcaaag aaccttgccc agaggcatcg gtgttgatca
    10201 ttccgcctaa agtcgctcgg ttactggtag aaagttcggg ggaaaagaaa agtccatgtg
    10261 gttttaaaaa ttgattgagc tggtctttca ccacgccagc ctgcactcga acccaacgct
    10321 cttccacatt cagttccaaa atctgattca tatggcggga gagatcaaca ataatattat
    10381 tgttaagaga ttgcccgtta gtgcctgtgc caccgcctcg tggagtaaaa gttaaatgtt
    10441 gatattctgg cttttgggca agtttggtta gaattacgac atcagaaacg gatttcggaa
    10501 acagaatagc ttgcggaatt tgctgataca cgctgttgtc tgttgccagt gaaagcctat
    10561 ctgcataatt tgatgcaatt tccccactaa aatgctgatt gacgagttca gttaaatagg
    10621 attgggtggc tttatccagt tgcggtactt cagagagtct aggtatcata agctgtgttt
    10681 ggttttttat aataaaagca ctaaattttg cttatcttag caat
//
LOCUS       DANLXS010000079        10596 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.079, whole genome shotgun sequence.
ACCESSION   DANLXS010000079 DANLXS010000000
VERSION     DANLXS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10596
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.079"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..576
                     /locus_tag="PXD71_001461"
     CDS             <1..576
                     /locus_tag="PXD71_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL6291574.1"
                     /translation="GAYLEASEAQIFRGKYQEMEFVPNPDFDGPYYGLDFGFAQDPTA
                     AVKCWVFNGDLYIEFEAGKVGLELDDTADFIAKGVPQIADHVVRADSARPESISYLKR
                     HGLPRIIGVEKWKGSVEDGIEHIKSYGKVFIHPRCQQTLNEFRLYSYKTDRLSGDVLP
                     VVIDAHNHFIDALRYALTPLMQVKSAKGVLL"
     gene            573..1949
                     /locus_tag="PXD71_001462"
     CDS             573..1949
                     /locus_tag="PXD71_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDL6291575.1"
                     /translation="MSLEQDRLAFLAEALGLGNIKRRTLWKEFGYPNTLTFNHFLKAY
                     KRNSIAFAAINRLLDGCWVDCPVIVEGEQKNESKQTTEWESKVERFMKRYWSAIKEAD
                     RRNLVGNYSALLLQVRDGQQWDQPILQGALSSIGEFGLVKLIPVWQSQLSVTEFQEDV
                     TADNYGEPLMYQFSESAFGKKSVSRNIKVHASRVILLNEGGDFNSPHSGVPLLEAGYN
                     KLVDLEKTSGGSAEGFLKNASRQLGIKLTKDVDLRQLEDSAKALGFSSFSDALNDKIK
                     KINSGTDSALITHEGDASVLSVAPADPKPTWEISANEFAASVQIPFTILFGQQTGRLA
                     SDEDKTDWANRCNGRRNGFLTDVITQLLNRLWFIGVLPMPKNSDVTVSWSDLLAPSEK
                     EKIANAQALASVATTSQSAFGFAAVTANEIRESLGFEPLPDNLIPPTIDDENEDQDEN
                     QATADSDK"
     gene            1903..2841
                     /locus_tag="PXD71_001463"
     CDS             1903..2841
                     /locus_tag="PXD71_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL6291576.1"
                     /translation="MKIKTKIKPLRIPTNKADPLNMGVSVAKLFRHIDVIYSQIKRSI
                     KQQILQKMSLRLQANNFEYFPSYLTATELADLLETLQKIIDKEILSNGSQGENLWFDS
                     YIDEAALKGTQSAVTDLGLQSEAYRSQRNLSSIVFSPAYFNRLAIAHMASYSEWQGLS
                     DELRKSLAGVITEAVLNGKNVKETAREIRQKLDVSSKRAKRIAQSSQLAAYRRAEWQE
                     AEEAKEELGLNTKLLHFSALKATTRLAHAQRHGKYFDVAEVREWYSRDGNQFNCYCKQ
                     SVVVVNADGKTDIEPLLVGLAKERKQWVGALKNVKK"
     gene            2828..4198
                     /locus_tag="PXD71_001464"
     CDS             2828..4198
                     /locus_tag="PXD71_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDL6291577.1"
                     /translation="MSKNNVNIVTVINSKNISTESIDGDQHLIIRGVVPIVDDVVMND
                     GLYPADEINKSYNSLEGNFMPLGHPKIEGKYVSAQDVRAVNKHHVGAWAKNVRKESGK
                     VLVDMYINRRFAEGSEQGKTLLERLDGMVTNTNAEPIHVSTGLILNKVKQSGKSKGKK
                     YSWIATNMSFDHIAILLDVPGAATPDDGVGIFVNQDGSEMGVENVNLTQAADYRTEKL
                     FDKVRFYLMANSDLSFEEIHRALREAIRPEGKDKWSFWVEAVYPKYFIYSDDNSGKKY
                     KRSYFIDENDNVQLTSEPVEVVKKIGYEEITTNEEHNPMKTKILAVLNAANVKTEGLN
                     DDQLLEAYNKLQADKGKEPEKPKNEDDSEIDEKIKKAVNAAIEPLQQALQANTDKEQA
                     AMRAAVKAKFNMSDAAVNALQGEVLTELYAQTQQSVGLNNNSANGNGDNQWDGYSLNQ
                     EEKGNE"
     gene            4191..4625
                     /locus_tag="PXD71_001465"
     CDS             4191..4625
                     /locus_tag="PXD71_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291578.1"
                     /translation="MSNVIYRGSVKREPQTVNIVINDTSAPGAVVKLHNGKLEAAADL
                     KGRRFLLGNNRFKGQTIEQAYAKGDTATAFRLEPEQEYYAQIADGTYNFGDELTVKQA
                     GGKLTKAETGDVVLFFFDEESQRVISGGKGYADVVVANAYTK"
     gene            4640..5629
                     /locus_tag="PXD71_001466"
     CDS             4640..5629
                     /locus_tag="PXD71_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HDL6291579.1"
                     /translation="MLKFTKEQERFVINERINYDKKHALMAANSAGLLGNAMTLPKDV
                     WAEWDRDAVQIQREELVVFNDLAGISKSMPIGKLIHHFQTVSDSGSVNISLDGRSSAK
                     TDNVVLNYHGTPLPIIDSTFSFGWRDMSAAQSEGYQIDGAARANSLRKVAEKMEDLAL
                     NGDSSIVVGNAKLYGLRTAPNRMTNTHNLDLATATPKEIYDVFRDLISKFHAKNYYSP
                     VTLYVNYGDYFAMSTRDYSEQKSEGSILNKVMTIPQIAKIVPASRVPQNEILGLCKRS
                     DVYQVLNGMPLVTRPIARHNETDDYSFQIMAAVAVEFKFDDKGNAGYIQYTKS"
     gene            5641..5901
                     /locus_tag="PXD71_001467"
     CDS             5641..5901
                     /locus_tag="PXD71_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291580.1"
                     /translation="MKWLLTHDSHELKKGDVYEGETLPLWLVGKAIEIEEKSFEVATP
                     SDHADGETGETGETGETGETGETGETGETGETGQKGKKDKGK"
     gene            5904..6281
                     /locus_tag="PXD71_001468"
     CDS             5904..6281
                     /locus_tag="PXD71_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291581.1"
                     /translation="MAAEIELSEARTTLDELGFTSPNSLLELYVQQTNTLDQALNKSG
                     YSEETQKLIKLYLVAILAISSGARRIKSEGAPSGASRTFEYDPNALDNLKSAIRSIDP
                     KGCAESLLPKDKVVGFFDVVGGC"
     gene            6281..6625
                     /locus_tag="PXD71_001469"
     CDS             6281..6625
                     /locus_tag="PXD71_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291582.1"
                     /translation="MSNLSNWSYTAKATLWQAKGKNDDGVLVFSTPQIISCDYGADRK
                     RARFEIGREQAVKNVIWTEFAHAKLGDYVLIGESEQADPILAGAEEIIHIQRFADTFD
                     RNRDDFALITGG"
     gene            6627..6995
                     /locus_tag="PXD71_001470"
     CDS             6627..6995
                     /locus_tag="PXD71_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL6291583.1"
                     /translation="MGIKVKGIRQVKQRLNQVVGEVQSQKATRAMFRILNAVAPLAAH
                     YTPVDTSTLINSQFTEMEVNGTRLIGRVGYSANYAAYVHDPNIKQNFRKPSAKKEFLT
                     SALNESKATIKSIVKEELSL"
     gene            6992..7372
                     /locus_tag="PXD71_001471"
     CDS             6992..7372
                     /locus_tag="PXD71_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291584.1"
                     /translation="MISYVKAFKQWLEAHSLADGYVVQLYQWEDTQKAKPVIVIQPNS
                     GSPQVSDLSSEHYLLISLVAGKNSGYTIEERARAIMSKILVEPFASFGYIESMGGLPA
                     PIFTEDNRMIFRLPLRIISTNQEE"
     gene            7376..7858
                     /locus_tag="PXD71_001472"
     CDS             7376..7858
                     /locus_tag="PXD71_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6291585.1"
                     /translation="MGSPSPQNNAENLVVGRAVVLEYGTTDAKPQESEWKVAGAMTTK
                     SWDFSPNSVTSEADDAGGFPETLITNSDFSISGDGEWRKRPKSKELGIKDLVVLYANA
                     VKNRTQPYIWVRLKYAGLTILGKMVITALSSEAPTNDLVKFSIEFKVGDSSTIEIAAA
                     "
     gene            complement(7898..8314)
                     /locus_tag="PXD71_001473"
     CDS             complement(7898..8314)
                     /locus_tag="PXD71_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL6291586.1"
                     /translation="MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLL
                     SCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKVLLHNTMQEQAVSKAEIARLTNI
                     RPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG"
     gene            complement(8370..8546)
                     /locus_tag="PXD71_001474"
     CDS             complement(8370..8546)
                     /locus_tag="PXD71_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL6291587.1"
                     /translation="MKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELK
                     TGLVEGVKKQLGLK"
     gene            8642..9313
                     /locus_tag="PXD71_001475"
     CDS             8642..9313
                     /locus_tag="PXD71_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291588.1"
                     /translation="MKMVSYPITEIGEMLIATDGRDFLFRPSFKNIYKIGSPQEIVKI
                     YALLHGCEIREILPHILTKSESHQSYFWSVLNSPVFGRKILQNAMIVMSCCCDDDISL
                     LIGEWLPSKSGVRYKLGKMPPETIINFAKNLMGHGVIGCCELPKKEQSAKNEFSESFD
                     AVEYISLARTHFNMSREDAENLTMTELQQLLKSQMPAENNSYGQFTEEEYDRIMAEYE
                     KRRAA"
     gene            complement(9328..9561)
                     /locus_tag="PXD71_001476"
     CDS             complement(9328..9561)
                     /locus_tag="PXD71_001476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291589.1"
                     /translation="MKKDCTKQDFANWLNENQEVCWIKEVSIERILSCVDDYVAICNA
                     RSKDEFELLNDIRELFYSAHYKDFTPVLSGRFF"
     gene            9730..10188
                     /locus_tag="PXD71_001477"
     CDS             9730..10188
                     /locus_tag="PXD71_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291590.1"
                     /translation="MKKMIVFVASVLSSFVIASDWEIIEAAQNKVKAELIRKSEKKAE
                     LCLNVAQAVLITNQKVQKDTFSMCGEFSTEIVFSDSKAYSLAVCGKVKGKDIIGNPIE
                     SDYIYQRATKDFYFNYNGKKSAYYDPQSDLARLQEVSDSMFNAMQNRYCK"
BASE COUNT         3193 a         2154 c         2487 g         2762 t
ORIGIN      
        1 ggtgcttatt tggaagcgag cgaagcgcaa atctttcgcg gtaaatatca agaaatggaa
       61 tttgtgccga accctgattt tgatggccca tattacggct tagattttgg tttcgcgcaa
      121 gatccgacgg cggcggtcaa atgctgggta tttaatggcg atctgtacat cgagtttgaa
      181 gcaggcaaag tgggcttaga acttgacgac accgcagact ttattgccaa aggcgtgcca
      241 caaattgccg atcacgttgt gcgtgcagat tcggcgcgtc ctgagtcaat tagttacctc
      301 aaacgtcacg gcttaccgcg cattatcggt gtggagaagt ggaaaggctc ggtggaagac
      361 ggcattgagc atattaagtc ctacggcaaa gtctttattc acccacgctg tcagcaaacg
      421 ctcaacgaat ttcgccttta ttcgtacaaa accgaccgct tatcgggcga tgtattgcct
      481 gtcgtgattg atgcccataa ccactttatt gatgcgctac gctacgcatt aacgccgttg
      541 atgcaagtta aaagtgccaa aggagtttta ctatgagcct agagcaagac agactggctt
      601 ttttagccga agccctaggg cttggcaata tcaaacgaag aacgttatgg aaggagtttg
      661 gctaccctaa taccttaaca ttcaaccatt tcttaaaagc ctataagcgc aatagtatcg
      721 cttttgcagc gattaaccgc ttacttgacg gttgttgggt tgactgcccc gtaatcgttg
      781 agggtgagca aaagaacgaa tcaaagcaga caaccgaatg ggagtccaaa gttgaacgtt
      841 ttatgaaacg ctactggtcg gcaatcaaag aggcagatag acgaaacctt gtcggtaact
      901 attcggcgtt attacttcaa gtaagagatg gtcaacaatg ggatcagcct attttgcaag
      961 gcgcgctatc tagcattgga gagttcggac tggttaaact gatccccgtg tggcagtcac
     1021 agctttcagt gacagagttt caagaggatg tgaccgccga taattacggc gaaccgctga
     1081 tgtatcaatt cagcgaatct gcctttggca aaaaaagcgt gtcacgaaat atcaaggttc
     1141 acgcaagtcg tgtaattttg ctcaatgagg gtggcgactt taattcccct cattctggag
     1201 ttcctctgct tgaggcgggt tacaacaagc tagttgatct cgaaaaaaca tcgggcggca
     1261 gcgccgaagg ttttctgaaa aacgcaagcc gacaattagg gatcaaatta acaaaagatg
     1321 ttgatttgag acagctcgaa gacagcgcaa aagcccttgg gttttcttca ttttctgatg
     1381 cgttgaacga caagatcaag aaaatcaact caggaacaga ctcagccttg attactcacg
     1441 aaggtgatgc ctctgtgttg tcagtcgcac cagcagatcc gaagccaacg tgggaaattt
     1501 ctgcaaatga gtttgccgcc tcagtacaaa tccctttcac gattttgttc ggacagcaaa
     1561 cagggcgatt ggcaagtgac gaggacaaaa ccgactgggc aaaccgttgc aacggtcgtc
     1621 gaaatggttt tctcaccgat gtgataacac aactacttaa tcgcttgtgg ttcatcggtg
     1681 tgctacctat gccaaaaaat agcgatgtca cagtgtcttg gtctgactta cttgcaccaa
     1741 gcgaaaaaga gaaaatcgca aacgcacagg ctttggcttc agttgcaact acatcgcagt
     1801 ctgcctttgg ttttgccgcg gtaacagcga acgaaatccg cgaatcttta gggtttgaac
     1861 ctctgcctga taatctgatt ccgccaacaa ttgacgacga aaatgaagat caagacgaaa
     1921 atcaagccac tgcggattcc gacaaataaa gccgatccgc tcaatatggg agtgtcggtt
     1981 gccaagctat ttcgtcacat tgatgtcatt tactcacaaa ttaagcgctc cattaagcaa
     2041 caaatcttgc aaaagatgtc actgcgactg caagcaaata attttgagta ttttccttcg
     2101 tatcttactg cgactgagtt ggcggatttg cttgaaacct tgcaaaagat tatcgacaaa
     2161 gagattttgt cgaatggttc tcaaggcgaa aatctctggt ttgactccta tatcgacgaa
     2221 gcagcactaa aaggcactca gtctgcggtc actgatttag gtctgcaatc agaggcttat
     2281 cgcagtcagc gaaacttatc atcaatcgtt ttcagtcctg cgtacttcaa ccgattggcg
     2341 attgcacaca tggcaagtta tagcgagtgg caaggtttaa gcgacgagtt gcgaaaatct
     2401 ttggcggggg tgatcacgga ggcggtttta aacggcaaaa acgtgaaaga aactgcccgt
     2461 gagattcggc aaaaacttga tgtgtcatca aaacgggcga agcgaatagc gcaatcaagc
     2521 cagttggcgg catatcgtcg ggcagaatgg caagaggcgg aagaggcaaa agaagaactt
     2581 gggctaaaca ccaagctctt acatttttca gcgctcaaag ctactacgcg attagcacac
     2641 gcccaacgcc acggcaaata cttcgatgtt gcagaggtgc gcgaatggta tagccgagac
     2701 ggcaaccaat tcaactgtta ctgcaagcag tctgtggttg tggtcaacgc cgacggtaaa
     2761 acagatattg agcctctcct tgttgggttg gctaaagaac gtaaacaatg ggtaggagca
     2821 ttaaaaaatg tcaaaaaata acgtgaacat cgtcaccgtc attaacagca aaaatatcag
     2881 cacagaatct attgacggag atcaacacct gattatccgc ggcgttgtgc cgattgttga
     2941 tgatgttgtg atgaacgacg gactttatcc tgcggatgaa atcaataaaa gctacaacag
     3001 cttagagggc aactttatgc cgcttgggca tccaaaaatc gaaggtaaat acgtttcagc
     3061 ccaagatgtt cgagcggtga acaaacatca cgtcggtgcg tgggcgaaga acgtacgcaa
     3121 ggaaagtggc aaagtgctgg tcgatatgta tatcaaccgc cgctttgcgg aaggttcaga
     3181 acagggcaaa accttgcttg aacgacttga cggaatggtg acaaacacca acgctgaacc
     3241 tattcacgtt tccacagggc tgattctaaa caaagtcaag caatcgggta aatccaaagg
     3301 caagaaatat agctggattg cgacaaatat gtcgttcgat catattgcaa tattgcttga
     3361 tgtgccgggc gcggcgacac ctgatgatgg cgtggggatt tttgtaaacc aagacggaag
     3421 cgaaatgggg gtggaaaatg taaacctaac acaagcggcg gattaccgca cagaaaagct
     3481 gtttgacaaa gtacgatttt acctgatggc gaactcagat ttatcgtttg aagaaattca
     3541 tcgcgcgctg cgtgaagcta ttcgccccga aggcaaagat aagtggtcat tttgggtcga
     3601 agctgtttac ccgaaatact tcatctattc ggacgataac agcggcaaga aatacaagcg
     3661 gagctatttt attgacgaaa atgacaatgt gcagctcact agcgaacctg tcgaagttgt
     3721 caagaagatt ggctacgaag aaattacaac caatgaggaa cataatccaa tgaaaaccaa
     3781 aatcttagcc gtgttgaatg cggcaaacgt gaaaacggaa ggcttaaatg acgatcagct
     3841 cttggaagcc tacaacaagc tgcaagcgga taagggtaaa gagcctgaaa agcctaaaaa
     3901 tgaagatgac agcgaaattg atgagaaaat caagaaagca gtcaatgccg cgattgagcc
     3961 actccagcaa gccttgcaag ctaatactga caaagagcaa gcagcaatgc gtgcggcggt
     4021 gaaagccaag ttcaatatga gcgatgccgc agtcaatgct ttgcaaggcg aggtattaac
     4081 tgagttgtat gcgcaaacgc aacaatcggt cggattaaac aataattcgg caaacggcaa
     4141 cggcgacaat caatgggatg gttactcact caaccaagag gagaaaggca atgagtaatg
     4201 taatttatcg cggttcggtc aagcgtgaac ctcaaacggt gaatatcgtg atcaacgata
     4261 cttcggcacc gggtgcagta gttaaactcc acaacggcaa attagaggcg gcggctgatt
     4321 taaaaggtcg ccgtttttta ttgggcaaca accgctttaa ggggcagaca atcgaacaag
     4381 cgtatgcgaa gggcgatact gcaacggcgt ttcgtttaga gcctgaacag gagtattacg
     4441 cgcaaattgc cgacggcact tataactttg gcgatgaact cacggtaaaa caagcgggcg
     4501 gcaaactgac caaagcggag acgggcgatg tagtgctatt tttcttcgac gaagaatcgc
     4561 agcgtgtaat cagtggcggc aaaggctatg ccgatgtcgt ggtggcaaac gcctacacta
     4621 aataagaggt aacaaagcaa tgttgaaatt cacaaaagaa caagaacgct tcgtgattaa
     4681 cgagcggatc aattacgaca aaaaacacgc gctaatggca gcaaattcgg cggggttatt
     4741 gggcaacgcg atgactttgc cgaaagatgt ctgggcggaa tgggatcgtg atgcagtgca
     4801 aattcagcgc gaagaattgg tggtgtttaa cgatttagcg ggcatttcaa aatcaatgcc
     4861 gattggcaaa cttattcacc atttccaaac ggtatctgat agcggttcgg ttaatatctc
     4921 attagacggt cgttcatcag cgaaaaccga taacgtggtg cttaactacc acggtacacc
     4981 gttgccgatt attgattcca ctttctcttt tggctggcgc gatatgtcgg cagctcaaag
     5041 cgaagggtat cagatcgacg gtgcagcacg cgcgaacagt ctccgcaagg tggctgaaaa
     5101 aatggaagat ctcgctttaa atggtgattc ctctattgtg gttggcaatg ccaagcttta
     5161 cggcttgcgt actgcgccaa atcgtatgac taacacacac aatctggatt tagccactgc
     5221 gacaccgaaa gaaatttacg atgtgttccg tgacttgatt agcaaattcc acgcgaagaa
     5281 ctactactct cctgtaacgc tttatgtgaa ctatggcgac tatttcgcaa tgagtacacg
     5341 tgattactcc gagcagaaat ccgaaggatc tatcttgaac aaagtgatga cgattccgca
     5401 aattgcgaaa atcgtgccag caagccgtgt accgcaaaat gaaattctcg gcttgtgcaa
     5461 gcgttcggat gtgtaccaag tgcttaacgg tatgccgtta gtgactcgtc cgattgcgcg
     5521 ccataatgaa accgatgatt actcgttcca aattatggca gccgttgccg tggagttcaa
     5581 attcgacgat aaaggcaatg ctggctatat ccagtacact aaatcctaag gcggtgcaaa
     5641 atgaagtggc tattaacaca cgatagccac gagttgaaga aaggcgatgt ttacgaaggc
     5701 gaaacattac cgctttggct tgtgggcaaa gctatcgaaa tcgaagaaaa atcctttgaa
     5761 gttgcaacgc cttctgatca cgctgacggc gaaactggcg aaactggcga aactggcgaa
     5821 actggcgaaa ctggcgaaac tggcgaaact ggcgaaactg gcgaaactgg tcagaaaggc
     5881 aaaaaagata aaggcaaata actatggcag cagaaataga gctatccgaa gcgcgaacaa
     5941 cgcttgacga actgggtttc acttcgccga actctctgct tgagctttac gttcagcaaa
     6001 cgaacacctt agaccaggcg ttgaacaaat cggggtacag cgaagaaaca cagaaactga
     6061 tcaagctcta tctggttgct attcttgcaa tttcatctgg ggcgcgccgt ataaagtcag
     6121 aaggtgcgcc tagcggtgca agccgaacgt ttgagtatga tccaaacgcg ctagacaacc
     6181 ttaagtcggc gattcgctca attgatccga aaggttgcgc tgaaagtttg ctacccaaag
     6241 ataaagttgt cggctttttc gatgtggtcg gaggctgcta atgtcaaatt tatcgaattg
     6301 gtcctacacc gcaaaagcca cgctttggca ggctaaagga aagaacgatg acggcgtttt
     6361 agttttttct acgccacaaa tcatttcctg tgactacggt gcagatagaa agcgcgcacg
     6421 ttttgagatt gggcgtgaac aagcggtcaa aaatgtgatc tggacggagt tcgcccacgc
     6481 caaattaggc gattatgttc tgattggcga aagcgagcaa gccgatccga ttttggctgg
     6541 cgcagaggaa attattcata tacagcgctt tgctgatacg ttcgaccgca atcgtgatga
     6601 ttttgcgctg ataacaggcg ggtaaaatgg ggatcaaagt aaaaggcata agacaggtca
     6661 agcagcggct gaatcaggtt gttggcgagg ttcagagcca aaaagcaact cgggcaatgt
     6721 ttcgcatttt gaatgcggtt gcccctcttg cggcgcacta tacgcctgtt gatacctcaa
     6781 cgttgatcaa cagtcaattc actgaaatgg aagtgaacgg cacacgcttg attggaagag
     6841 tgggttattc tgctaattat gcggcttacg ttcacgatcc taatatcaag caaaacttcc
     6901 gcaagccttc agcgaagaaa gagtttttga cttccgcgct gaacgaatca aaggctacga
     6961 tcaaatccat tgttaaagag gagttgtcac tatgatttct tatgtcaaag ccttcaaaca
     7021 atggttagag gctcactctc ttgcagacgg ctatgttgtg cagctttacc agtgggagga
     7081 tacgcaaaaa gccaagcctg ttattgttat tcagccaaat agtggttcgc cgcaagtttc
     7141 tgacctgagc agcgaacact atctgctaat tagccttgtt gcaggtaaaa attcaggtta
     7201 cacaatcgaa gagcgagcaa gggcgattat gtcaaagatt ttagttgagc ctttcgcttc
     7261 ctttggttac atcgaatcaa tgggcggatt acccgcccca attttcaccg aagataatcg
     7321 aatgattttt cgcttaccac ttcggattat ttcaacaaat caagaggagt agtttatggg
     7381 ttctccatct ccacaaaata acgcagaaaa tctcgtggtg gggcgtgccg tagttttaga
     7441 gtacggtacg actgatgcaa agccacaaga aagcgaatgg aaggttgcgg gcgcaatgac
     7501 gacaaagagt tgggatttta gcccaaattc ggttacatca gaggctgatg atgctggcgg
     7561 cttccctgaa accttaatta caaactcaga ctttagtatt tctggtgacg gtgagtggcg
     7621 caaacgacca aaatcgaaag aactaggcat caaagatcta gttgtgttat acgcaaatgc
     7681 ggtaaaaaat cgcacccaac cttacatttg ggttcgtttg aaatacgcag gcttaacaat
     7741 tctcggaaaa atggttatca ctgcattaag tagcgaagcc ccaaccaatg acttagtgaa
     7801 attctctata gagttcaagg ttggcgattc aagcactatc gaaattgctg cagcgtaaaa
     7861 aatcccaccc tctataacaa gagcggtggg attatcatta tcctagggat agttgtaggg
     7921 gctttcctaa gctagccaaa atacgaccaa tagtgtcgat ttttgtaata tggcgagggg
     7981 ctaaaattcg ctgtacttct ggcggacgga tattagttaa tcgggcaatt tctgccttgc
     8041 ttaccgcttg ttcttgcatt gtattatgca aaagcacttt tgcgtagatg ctctctggca
     8101 taaacacagg cacttcgtcg gtatttactg ggcgagtgag cggaaagcgt ctatcttcat
     8161 cgaaataaat ttcaacacaa gatagtagca cgtcttctgc catttccatt gcttcttcaa
     8221 atgtatcgcc ttgggtgata gcttctggca aatcaggaaa tgtcactaca aagccccctt
     8281 cttcagcggg ggtaaataac gctggataaa acatattttc tccttattca agggtgtagt
     8341 gaaagcccct taacatcagg ggctttatat tattttaagc ctagttgctt tttaacccct
     8401 tccactaagc ctgtttttaa ctcttggctt gggtgtttgg ggaggtggct tcttttgccc
     8461 ttgtagtaga gtttcaaatg ctttgatcca ttttcaacct ctaccccatt agcctttagc
     8521 caccttagga attcgctttg cttcactacc tcctcctatt tgtttaactt gggttcatta
     8581 tatgcaaaaa tgctaacttt gtcaacagaa agttagcaaa aatgctaatt tttttgttga
     8641 ggtgaaaatg gttagctatc caattactga aattggtgaa atgcttattg caaccgatgg
     8701 gcgagacttt ttattcagac cgagtttcaa aaatatctac aaaattggaa gcccacaaga
     8761 aattgtaaaa atctatgcac tattgcacgg ctgtgagatt cgagagatat tgccgcacat
     8821 tttaaccaaa tcggagtctc atcaatctta cttttggtca gtgctgaatt cgcctgtttt
     8881 tggcagaaaa attctgcaaa acgcaatgat cgtgatgtcg tgctgttgcg atgatgatat
     8941 ttccctgttg attggcgagt ggttgccgtc aaagtctggc gttaggtaca agttgggtaa
     9001 aatgccacct gagacaatta tcaattttgc aaaaaattta atgggtcacg gagtgattgg
     9061 ttgctgcgaa ttgccaaaga aagaacaatc tgcaaaaaat gaattttcag aaagttttga
     9121 tgctgttgag tacataagtt tagcccgaac tcatttcaat atgagccgcg aagacgctga
     9181 aaatctcacg atgaccgaat tgcagcaact tctaaaaagt caaatgcctg ccgaaaataa
     9241 tagctatgga cagttcaccg aagaagagta cgacagaatt atggcggaat atgagaaaag
     9301 aagagcggct taaatagccg ctaacattta aaagaaccta cctgatagaa cgggagtgaa
     9361 atccttgtaa tgtgctgaat aaaaaagctc tcttatgtca ttgagtagct caaattcatc
     9421 tttggatctt gcgttgcaga ttgccacata gtcatctacg caggataaaa tacgttcaat
     9481 actgacttct tttatccagc atacttcttg gttttcattc agccagttgg caaagtcttg
     9541 tttagtgcag tcttttttca taagttctcc tttttgatta tttgcaaaaa aaatcatatc
     9601 aaatcatccg ttgttatcta ttggcgaaaa taatttttgc gacacagatc gcaaaaatag
     9661 gttttgacgg tggaaaaacg aatcttaatt cgtagaatgg taaaatttct ttatttttaa
     9721 ggtattttta tgaaaaaaat gattgttttt gtagcttctg ttttgtcatc ttttgttatt
     9781 gcatcggatt gggaaataat agaagcggct caaaataagg tgaaagcaga gcttataaga
     9841 aaatcagaaa aaaaagcaga gttatgttta aatgttgctc aggctgtctt aattactaat
     9901 caaaaagtac aaaaagatac tttctcaatg tgtggtgaat tttcgacaga gattgtcttt
     9961 tctgactcta aagcatatag ccttgcagta tgtggaaagg ttaaaggtaa agatattatc
    10021 ggaaatccga tagagagcga ctatatatac caaagagcaa ctaaggattt ttactttaat
    10081 tataatggca aaaaatcagc ttattatgat cctcaatctg atcttgcaag gcttcaagag
    10141 gtctcagact ctatgtttaa tgctatgcaa aatagatatt gtaagtgatg cttgcattgg
    10201 tttttaaata tttaactcaa ataatttatg tgtttttagt ttaatagggc gatttatgga
    10261 tgtaaaggta attaacgtct tactaattat tcttttttca gcaattgtaa taggtttatt
    10321 cctttttcaa gaatcaagat taagagatcc aaacaaccat tcagtgatat atttagagca
    10381 gattcaagaa atgtgcagct tagataaaat aggctcatac cagattgatt ttgtacgaca
    10441 aagtgggaat gagtacaaag agagtattct tgttaatgat tttgagttag caataaaaac
    10501 cgtattatcc acgttaagaa gggcaaaaat agatagtgtg ccagtaatat ctaacacact
    10561 tatttattat ccaccgagct ttctacaacg caagag
//
LOCUS       DANLXS010000080        10393 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.080, whole genome shotgun sequence.
ACCESSION   DANLXS010000080 DANLXS010000000
VERSION     DANLXS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.080"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            370..1014
                     /gene="rppH"
                     /locus_tag="PXD71_001478"
     CDS             370..1014
                     /gene="rppH"
                     /locus_tag="PXD71_001478"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDL6291591.1"
                     /translation="MIDFDGYRPNVGIVICNKHGQVLWAKRFGQNSWQFPQGGINEGE
                     NIEAAMYRELYEEVGLSKKDVRLLWASKYWLKYKLPKRLVRNEGSGPVCIGQKQRWFL
                     LQLIGDESLINLKTTKNPEFDGWRWVSFWYPVRQVVSFKRDVYRKAMKEFAQVLQNNI
                     QKATDDKTGEKPRIERGETSPHTYKKTSQDSAKAYKREARKPYRPWHQHNQKGK"
     gene            1016..1804
                     /locus_tag="PXD71_001479"
     CDS             1016..1804
                     /locus_tag="PXD71_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL6291592.1"
                     /translation="MTILLSCLFLGVFVGFLAGLFGIGGGLIIVPSLVFLLPMAGVPP
                     EYIMSTALGTSFSTIIITAFASAQRHHKLGNVDIQTSKYFIPALMVSVFLSGLVVSRL
                     DPQLMSRIFAVMVLYLAGKMIFSIKRAPKNKPLTVQTTIIGGSIIGALASMAGIAGGA
                     FIVPFLNDRGLEMKRAIGTSSFCGAFLGLSGTISFIVSGWGLEGMPDYSLGYVYLPAL
                     FGITATSFFTSKMGASAANALPVPMLKKAFAVLLVCIAINMFLK"
     gene            1812..2609
                     /gene="lgt"
                     /locus_tag="PXD71_001480"
     CDS             1812..2609
                     /gene="lgt"
                     /locus_tag="PXD71_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL6291593.1"
                     /translation="MNEQFIQFPQISPIVFEIGPIALRWYGLMYLAGFAFAYWLGMRR
                     AKNSNGVWTTEQVDSLVYTCFWGVVLGGRIGDVFFYSFDRFLQDPLYLFRIWEGGMSF
                     HGGLIGVIVAMIWVSYRQKRNFWQTADFIAPLVPFGLGMGRIGNFINDELWGRVTDVP
                     WAVLFPSGGYLPRHPSQLYEAFLEGVVLLFILNWFIQKPRPAGAVSGLFLIGYGTFRF
                     IVEYFREIDHNVNTTADLITRGQLLSLPMIIGGILIMAWAYKVNKVK"
     gene            2696..3364
                     /locus_tag="PXD71_001481"
     CDS             2696..3364
                     /locus_tag="PXD71_001481"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDL6291594.1"
                     /translation="MSKKYKVIGFDLDGTLINTLPDLTLVANSMFLEAGLPTVTQEKV
                     LTWIGKGADIFFQNAVAHTGRIFQADELVKMRVSFDKYYATYVCEESTLFPNVKETLE
                     ILKANDYPLVVITNKPTKLVEPVLSAFGIYHLFDETLGGQSLPKIKPHPDPMFFICEK
                     FGITPSELLFVGDSENDVIAAKAAGCDVVGLTYGYNYNVPIEQSNPTFVTSEFKDVLK
                     IVGL"
     gene            3434..3694
                     /locus_tag="PXD71_001482"
     CDS             3434..3694
                     /locus_tag="PXD71_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL6291595.1"
                     /translation="MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVI
                     NPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPAIGKEIL"
     gene            3691..4023
                     /locus_tag="PXD71_001483"
     CDS             3691..4023
                     /locus_tag="PXD71_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL6291596.1"
                     /translation="MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVP
                     ITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTVDIAQRHGKFIEALPESLLDEIM
                     ARVSVIFE"
     gene            4150..5181
                     /gene="trpS"
                     /locus_tag="PXD71_001484"
     CDS             4150..5181
                     /gene="trpS"
                     /locus_tag="PXD71_001484"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL6291597.1"
                     /translation="MSKPIVFSGVQPSGELTIGNYLGALRNWVKMQDDYDCVYCIVDQ
                     HAITVRQDPESLRKSTLDVLALYLACGIDPNKSTIFIQSHVPEHAQLAWVLNCYTYFG
                     EMGRMTQFKDKSARYEENVNVGLFTYPVLMAADILLYQANQVPVGDDQKQHLEITRDI
                     ANRFNALYGKKDSEGNLIEPVFAVPEVFIAKSGARVMSLLEPTKKMSKSDDNRNNVIG
                     LLEDPKAVAKKIKRAMTDGDEPPVVRYDVQNKAGVSNLLDILSAITGKTIAELETEFE
                     GKMYGHLKTEVADKVSELLTNLQERYHHYRQDEALLEKIYREGAEKARAQAKATLDKV
                     YELVGFVRY"
     gene            complement(5245..6000)
                     /locus_tag="PXD71_001485"
     CDS             complement(5245..6000)
                     /locus_tag="PXD71_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDL6291598.1"
                     /translation="MKFIKKLSYSVFLATVITACATTESINQEAAQSYAQVKTQARQQ
                     NAIDNSSATAKRIHTVFHKMKPYAERANQTGVPFHWEITVLKSDELNAWAMPGGKMAF
                     YTGLVERLNLSNDEIAVVMGHEMAHALKEHGKSDRTVSAITSIVGAIADVAVTASTGV
                     STEGLLSTGVDLIATKPFSRSQETEADEVGLILMAESGYNPSAAPNVWVKMSKANGDS
                     GLSIFSTHPSNADRQENLARLVPEAMKIYNARK"
     gene            complement(6056..6583)
                     /gene="ppa"
                     /locus_tag="PXD71_001486"
     CDS             complement(6056..6583)
                     /gene="ppa"
                     /locus_tag="PXD71_001486"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDL6291599.1"
                     /translation="MGLELVPAGKELPDDIYVVIEIPANSDPIKYEVDKETGALFVDR
                     FMATAMFYPANYGYVNNTLSSDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGG
                     DAKVVAVPHTKLSKEYDHIKDVGDLPALLKAQIQHFFESYKALEAGKWVKVEGWGDVN
                     EARQEILESFERAKK"
     gene            6800..6875
                     /locus_tag="PXD71_001487"
     tRNA            6800..6875
                     /locus_tag="PXD71_001487"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6833..6835,aa:Lys,seq:ttt)
     gene            6898..6973
                     /locus_tag="PXD71_001488"
     tRNA            6898..6973
                     /locus_tag="PXD71_001488"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6931..6933,aa:Lys,seq:ttt)
     gene            6995..7070
                     /locus_tag="PXD71_001489"
     tRNA            6995..7070
                     /locus_tag="PXD71_001489"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7028..7030,aa:Lys,seq:ttt)
     gene            7163..8197
                     /gene="mltG"
                     /locus_tag="PXD71_001490"
     CDS             7163..8197
                     /gene="mltG"
                     /locus_tag="PXD71_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL6291600.1"
                     /translation="MLKKILIALGLAGVIACGGLFYGYQKLTSLAEHPITVQPNQLFV
                     LEKGVSSQKLAALLEEQGIVTHDDADLIPYLMRLYPELSKFKAGAYSLAGLTTVKDLL
                     AHLSSGKEVQLNVQFIEGKTFKIWREQLAKADYLQHTLQGKSEKEIADLLGIPHEKIE
                     GWIAPDTYNYTPNSTDLALLKRAYQKQKTALEQAWKNRAENLPLATPYEMLILASIVE
                     KETGIATERPQVASVFINRLRNNMRLQTDPTVIYGMGDRYNGNIRRKDLEEATPYNTY
                     QIDGLPPTPIAMPSEASIKAVANPDNTPYLYFVADGTGGHKFSKTLNEHNKAVAEWIQ
                     IERKRKNEQK"
     gene            8208..8828
                     /locus_tag="PXD71_001491"
     CDS             8208..8828
                     /locus_tag="PXD71_001491"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL6291601.1"
                     /translation="MRGKFIVIEGLEGAGKTTAHQVILKELEQAGINDVVMTREPGGT
                     PLAEKLRHLIKHETEEAINDKAELLMLYAARVQLVENVIKPALAEGKWVLGDRHDMSS
                     QAYQGGGRKLDRHLLETLKESVLGTFEPDLTIYLDLDPVIGLERARGRGELDRIEQQS
                     LDFFYRTRERYLELTQHNDKAVIINAEQPIEQVTADIQQVIRNWLK"
     gene            8828..9805
                     /locus_tag="PXD71_001492"
     CDS             8828..9805
                     /locus_tag="PXD71_001492"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL6291602.1"
                     /translation="MYPWLESTYQQLTDSFLQGRGHHALLFKTDVGLGTDTLIYKVAE
                     WLLCSQKLRHQPCLECKSCLLMQSNNHPDFHILEPIEDKDIGIDQVRELIYKLQNFAQ
                     QGGNTVVYIRGVDRLTEASSNALLKTLEEPRENVYFLLEAPLQATVLATIQSRSQTWV
                     LYAPQASEVMAWLQTQFPAISTDDLEIALRLCHSRPLICKNFIENDRLQQRKTFLQTF
                     WRFYKSHDVFLLFQAFAQEKELILQQLEWLESFFSDALKAKMAISHGWLNPDLQNGIL
                     PFSQQLTTHGLLKGHQILQQTQRDLTEINAVNPELMLLDCLTKLVLVFE"
     gene            9936..10178
                     /locus_tag="PXD71_001493"
     CDS             9936..10178
                     /locus_tag="PXD71_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL6291603.1"
                     /translation="MARTTSVTLGEPLNDFVNEMVASGRYGSTSEVVRTALRMLEEKE
                     QYLAQLRQAIDDGDASGYATETIDEMMLRVKAELNV"
     gene            10171..>10393
                     /locus_tag="PXD71_001494"
     CDS             10171..>10393
                     /locus_tag="PXD71_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6291604.1"
                     /translation="MYKLSAQALRDQEKLFRYSISQFGVQQTESYFADLKHILNLLAD
                     FPTMGTSAEYIRGGLRMHTHQSHTIYYRIK"
BASE COUNT         3027 a         2278 c         2190 g         2898 t
ORIGIN      
        1 ttattcacgc ctaatttgaa aaactatcac atctttatcc acaaatggtg gggataactc
       61 tgctttattt gcgaagcaaa aattgccctg cttattcatt ttaaaattta aacttatttt
      121 gacgagaaaa aacgcaacaa cttgatcttt atccagatca ttgcttaaga aaaagatcgc
      181 atttttagat ctgattaatt gctctgtttt acaacgttcc gcctgtttat cctcaactta
      241 taccacttat attcaatagt tatccacaaa gttattcaca agcggttata ttttatggtt
      301 tttttgcaaa tcaacccaaa gtatgaaaca attctcccat tctagaattt ttatcaatta
      361 ggtggtcgag tgatcgattt cgatggctac cgcccaaatg ttggaatcgt tatctgtaat
      421 aagcacgggc aagttctgtg ggcaaaacgg tttggtcaaa actcttggca atttccgcaa
      481 ggtggaatta atgaggggga aaacattgaa gcagcaatgt accgtgagct ttatgaagag
      541 gtcggcttaa gtaaaaaaga tgtacgattg ctttgggcgt ctaaatattg gctaaaatat
      601 aaactgccaa aacgcttggt acgtaatgaa ggctctggtc ctgtctgtat cgggcaaaaa
      661 cagcgttggt ttctactgca attaatcggt gatgaaagcc tgattaattt gaaaaccacg
      721 aaaaatccgg agtttgacgg ctggcgttgg gtcagctttt ggtatccagt ccgccaagtg
      781 gtctctttta aacgagatgt ttatcgcaaa gcgatgaaag agtttgcaca ggttttgcaa
      841 aacaatattc aaaaagcgac tgacgataaa accggtgaga aaccacgcat tgagcgtggc
      901 gagacttcgc cccatactta taaaaaaacc tctcaggatt ctgccaaagc ctataaacga
      961 gaagctcgta agccttatcg tccttggcat caacataatc aaaaaggaaa ataagatgac
     1021 tattttatta agctgccttt ttttaggggt gtttgtcggc tttttagccg gcttgttcgg
     1081 cattggtggt gggttaatca tcgtgccgag tttggtcttc ctactgccca tggcaggtgt
     1141 accgccggaa tatataatgt ccaccgcact cggcacatca ttttcaacca ttattattac
     1201 cgcctttgca tcggcacaac gccatcataa gttaggcaat gtcgatatcc aaaccagtaa
     1261 atattttatt ccagcgttga tggtttcggt ttttctatcg ggcttggtgg taagccgcct
     1321 cgatccgcaa ctgatgtcta gaatttttgc ggtaatggta ctttaccttg caggtaaaat
     1381 gattttttca ataaaaagag caccgaaaaa taaaccactt accgttcaaa cgacgataat
     1441 cggaggttca atcatcggtg cgttagccag tatggcaggg atcgcaggtg gagcctttat
     1501 tgtaccattt ttaaacgatc gaggcttaga aatgaaacgt gccatcggca cttcctcatt
     1561 ttgcggagca tttttaggtt tatctggcac aatcagcttt attgtaagtg gctggggctt
     1621 agaaggaatg cctgattatt ctctcggtta tgtttattta ccggctctgt ttggtattac
     1681 ggcaacttca tttttcacct caaaaatggg ggcaagtgcc gccaacgctc tacctgtgcc
     1741 gatgctgaaa aaagcctttg cggtgctttt ggtctgtatc gccatcaata tgtttttaaa
     1801 ataaggacac tatgaacgaa caatttatcc aatttccaca aattagcccg attgtgtttg
     1861 aaatcggtcc gattgccctg cgttggtatg ggctaatgta tttagccggc tttgcctttg
     1921 cctactggct gggaatgaga cgggcgaaaa attccaatgg agtttggaca accgagcaag
     1981 tggatagctt agtctatact tgcttctggg gcgtagtatt aggtgggcgt atcggcgatg
     2041 tgttctttta tagcttcgac cgtttcttac aagatccgct ctacctcttt cgcatttggg
     2101 aaggcggaat gtcctttcac ggcggtttaa tcggcgtcat tgtagcaatg atttgggttt
     2161 cctaccgcca aaaacgcaat ttctggcaga cagcagattt tatcgcaccg ctagtaccgt
     2221 tcgggctagg tatggggcgt atcggcaatt tcattaacga tgaactttgg gggcgtgtaa
     2281 ccgatgtgcc ttgggcggtg ttatttccaa gcggtggcta tcttccccgc cacccgtcac
     2341 agctttatga agccttttta gagggtgtgg tgttactctt tatcctcaac tggtttattc
     2401 aaaagcctcg cccggcaggt gcggtttcag ggttattttt aatcggctac ggcacattcc
     2461 gctttattgt ggaatacttc cgagaaatcg accataacgt caataccacc gcagatttaa
     2521 tcactcgtgg gcaactactc tctttaccga tgattatcgg cggcatactg attatggcgt
     2581 gggcgtataa agtaaacaaa gtgaaataaa cgtaggggca agtcaatatt tgccctcatt
     2641 acaaaaattg caaaaaattt gcagaaaacg accgcttgtg tttgaaggat atactatgag
     2701 taaaaaatac aaagtcatcg gtttcgactt agacggaacg ctaatcaaca ccctccccga
     2761 tctcactctt gtcgccaatt caatgtttct agaagccggt ttgccaacgg ttacacaaga
     2821 aaaagtgctg acttggatcg gtaaaggggc agatattttc ttccaaaatg cggttgctca
     2881 caccggcaga attttccaag ccgatgagtt ggtgaaaatg cgagtgagct ttgataaata
     2941 ctacgccacc tatgtgtgcg aggaaagcac ccttttccca aatgtgaaag aaaccttaga
     3001 aatcctcaaa gcaaacgact acccgctggt ggtgattacc aacaaaccga ccaaattagt
     3061 cgagccggtg ctttccgctt tcggcattta tcatttattc gatgaaaccc ttggtgggca
     3121 atcgctcccc aaaatcaaac cgcaccctga cccgatgttt tttatctgcg aaaaattcgg
     3181 cattacccca tcagaattac tttttgtcgg cgactcagaa aatgacgtga ttgccgccaa
     3241 agccgccggt tgtgatgtgg ttggactaac ttacggctat aactacaatg tgccgattga
     3301 acaatctaac ccgacttttg tgacatctga atttaaagat gtgttgaaaa ttgtggggtt
     3361 ataaagaaaa catttgtttt cttttgatgt ttgctttaga ctttccgaac ctaaagattt
     3421 aaaggagtaa agtatgccaa caatcgcatt acgtcaggtc ggtggctcgg ttgcaatggt
     3481 gattccgcct gcttttttag aggcactcaa gctctccgtc ggtagcaaag tggatttgtc
     3541 tttaaacgac aaccaactcg tgattaatcc tgcccgccca aaagtgaaat acagcttaga
     3601 ggaattatta agacaatccg attttgaggc agcaaggaat gaagaaagcc aatcgtggtt
     3661 atccgagccg gcaataggaa aagaaatttt atgaaacgtg gcgatattta ctggctcgat
     3721 cttgatccaa ccttaggaca cgaacaatca ggcttccgcc ccgttgtaat tgtttccgca
     3781 actgcattta atctagccag taaactgcct gtggttgtgc cgattactaa cggtggagct
     3841 tttgctgaaa gactgggctt tgccgtttca ttaaaagatg ccggcttaaa aacaacaggg
     3901 attattcgat gcgatcaacc tcgcacagtc gatattgcac aacgccatgg aaaatttatc
     3961 gaagccttac ccgaaagcct acttgatgaa attatggcaa gggtttcagt gatttttgag
     4021 taagatacaa gcggtctgat ttgctctatt ttttgcaaaa tcatcaataa atctgaccgc
     4081 ttgtactttt atcccttccc aataaccgtt aagataacct atatttttca aatcaaacga
     4141 gaagataaaa tgagcaaacc tattgtattt agtggcgtgc agccctcagg tgaattgact
     4201 atcggtaact acttaggggc gttacgcaac tgggtgaaaa tgcaagatga ttatgactgt
     4261 gtatattgca ttgtcgatca acacgccatt accgttcgtc aagatccgga gtcgctacgc
     4321 aaatcaactt tagatgtgtt agccctgtat ttagcttgtg gcattgatcc aaacaaaagc
     4381 actattttta ttcaatccca cgtgccggaa cacgcccaat tagcttgggt gctgaactgt
     4441 tacacctatt tcggtgaaat ggggcgtatg acgcaattta aagataaatc agcacgctat
     4501 gaagagaatg tcaatgtggg cttatttact tatccggtat taatggcggc agatattctg
     4561 ctttaccaag ccaaccaagt gccggtaggc gatgaccaaa aacaacattt ggaaatcacc
     4621 cgtgacattg ccaaccgttt caatgcctta tacggcaaaa aagattccga aggcaacctg
     4681 atcgagcctg tttttgcagt gccggaagtc tttattgcga aatccggggc aagagtgatg
     4741 tcgctacttg agccgaccaa aaaaatgtca aaatcggacg ataatcgtaa caatgtgatt
     4801 ggactattgg aagatccgaa agcggtagcg aagaaaatca aacgggcaat gaccgatggc
     4861 gatgaaccgc cagtagtgcg ttatgacgta cagaataaag ccggtgtctc gaatttattg
     4921 gatattctct ccgccattac cggcaaaacc attgccgaat tagaaaccga atttgaaggc
     4981 aaaatgtatg gtcatttgaa aacagaagta gcagataagg tttccgaact attaaccaat
     5041 ctgcaagaac gttatcacca ctaccgccaa gatgaagcat tattggaaaa aatctaccgt
     5101 gaaggggcag aaaaagctcg agctcaagcc aaagcaacgt tagataaggt atatgaatta
     5161 gtcggttttg tgcgttacta acacttaatg aaaaatgctc ttgaggattc aagagcattt
     5221 ttgcaaaaaa gaaccgattg tcaattattt acgggcgtta taaatcttca ttgcttccgg
     5281 cacaaggcgg gctaagtttt cttgacggtc agcatttgat gggtgagttg agaaaatgga
     5341 caagcctgaa tcgccattgg ctttactcat ttttacccaa acattcggcg ctgctgatgg
     5401 gttgtagcct gattctgcca ttaaaattaa cccaacttcg tccgcctcag tctcttggct
     5461 gcgagagaat ggcttggtgg caatcaaatc cacgccagtg ctgagtaaac cttcggtgct
     5521 tacgcctgtt gaggcagtca cagccacatc tgcaatagca cctacaatac tggtaatagc
     5581 gctaaccgta cggtctgatt taccgtgttc tttcagtgcg tgagccatct cgtgtcccat
     5641 taccactgca atttcatcgt tcgataaatt aagtctttcc actaaccccg tgtaaaacgc
     5701 cattttaccg cccggcatcg cccacgcatt gagttcatcc gatttcaata cggtaatttc
     5761 ccaatgaaaa ggtacaccag tctggttggc tctttccgca taaggcttca ttttatgaaa
     5821 aacagtgtga atccgcttag cggtggcaga cgaattgtca atcgcatttt gctgacgagc
     5881 ttgggttttc acttgagcat aactttgagc cgcttcttgg ttaatgcttt ctgttgtggc
     5941 acaagcggta atcactgttg ctaaaaaaac tgaataactt aattttttga tgaatttcat
     6001 tttatatccc acaaaaaatg ccaaacaaat ttgcttggca ttcaaaaata aataattatt
     6061 tcttagcacg ctcgaatgat tctaagattt cttggcgagc ttcgtttaca tcgccccaac
     6121 cttctacttt cacccatttg cccgcttcta atgctttata gctttcgaag aagtgctgga
     6181 tttgtgcttt taataacgcc ggtaagtcac ctacatcttt gatgtggtca tactctttac
     6241 ttaatttggt gtgtggaaca gcaaccactt ttgcatcacc gccggcttca tcggtcattt
     6301 ttaacacacc gacaggacgg caacgaatca ccgaacccgg ttgtaacgga tatggtgttg
     6361 gcactaatac atcaaccggg tcaccgtctg atgataaggt gttattcaca taaccatagt
     6421 ttgccggata gaacattgcg gttgccataa agcgatccac aaataatgcc ccggtttctt
     6481 tgtccacttc atatttgatt ggatcagagt ttgccggaat ttcaattaca acataaatat
     6541 cgtccggtaa ttctttaccg gcaggaacta attctaagcc cattttggtt tccttctata
     6601 ataggtgaaa taaacggcgt gattatagcc tattttcaga aaaagataaa agcccgtttc
     6661 tcttttcatt ttccgccaac aaatccggct aattttttgc taaattgctt acaaaccgca
     6721 cactcaacca tttttcgata aaaaaaactt gccaaagttt taaaaatccg taagatatgc
     6781 cctcgttacg caagattatg ggtcattagc tcagccggta gagcagcgga cttttaatcc
     6841 gttggtcgaa ggttcgaatc cttcatgacc caccattaat cttcaaaaca cctctaaggg
     6901 tcattagctc agtcggtaga gcagcggact tttaatccgt tggtcgaagg ttcgaatcct
     6961 tcatgaccca ccattaattt caaaactcct ctgagggtca ttagctcagt cggtagagca
     7021 gcggactttt aatccgttgg tcgaaggttc gaatccttca tgacccacca tttactcaaa
     7081 tcccatttgc aaatttttct gaaaaaacaa ccgctttata gtagaattca gtcaattttt
     7141 atcactttta ttttattcat caatgctaaa aaaaatcctt attgcattag gcttagccgg
     7201 cgttattgct tgcggtggac tgttttacgg ctaccaaaaa ctcacctctt tagccgaaca
     7261 cccgatcacg gttcagccga atcaactttt cgtattggaa aaaggcgttt ccagccaaaa
     7321 attagcggct ctgttagaag agcaaggcat tgtcacccac gatgacgccg atcttatccc
     7381 ttatttaatg cgattatacc ctgagttaag caaattcaag gctggtgctt attcactcgc
     7441 aggcttaacg acagtaaaag atttgctcgc tcatttaagc tccggcaaag aagtgcaact
     7501 taacgtgcaa tttattgagg gcaaaacttt taaaatttgg cgggaacaac tagcgaaagc
     7561 cgattattta cagcacacgc tacaaggcaa atcggaaaaa gaaatcgctg acttactcgg
     7621 cattccacac gaaaaaatcg aaggttggat agccccggat acttacaact acacgcccaa
     7681 ttccaccgat ttagccttgc ttaaacgggc ttatcaaaag cagaaaacgg cgttggaaca
     7741 ggcttggaaa aatagagcgg aaaatctacc gcttgcaaca ccatacgaaa tgctgattct
     7801 tgcctcgatt gtggaaaaag agaccggcat tgcgaccgaa cgcccacaag tggcatcggt
     7861 ctttattaac cgtttacgca acaatatgcg actgcaaacc gatccaaccg tcatttacgg
     7921 aatgggcgat cgttataacg gcaatatccg ccgcaaagat ttagaagagg caacgccata
     7981 caatacttac cagattgacg gcttaccgcc aacgccgatt gccatgccga gtgaagcttc
     8041 gattaaagcg gttgccaacc cggataatac accctacctc tattttgtgg cagacggcac
     8101 gggcggtcat aaattcagca aaacactgaa tgagcataat aaagcggtgg cagaatggat
     8161 tcaaattgaa cgcaaacgca aaaatgaaca aaaatagtga gaacaatatg cgtggaaaat
     8221 ttattgtaat tgaaggctta gagggcgcag gcaaaacgac tgctcaccaa gtgattctca
     8281 aagagttgga acaagccggc attaatgacg tagtaatgac ccgagaaccc ggcggcaccc
     8341 cccttgccga aaagttacgc catttaatta aacacgaaac cgaagaagcc attaacgata
     8401 aggctgagct gctaatgctc tacgctgctc gtgttcaatt agtcgaaaat gtgatcaaac
     8461 ctgcccttgc cgaaggcaaa tgggttctgg gcgaccgcca cgatatgtct tcccaagcct
     8521 atcaaggcgg tggacgtaag ttggatcgtc acttactcga aaccttaaaa gaatcggtac
     8581 tcggcacatt cgagccagat ttaacgattt atctcgatct cgatccggtg attggtttag
     8641 aaagagccag aggcagaggc gaattagacc gcatcgaaca gcaaagcctt gacttcttct
     8701 accgcacacg agagcgttat ttagaattaa cccaacacaa cgataaagcc gtgatcatca
     8761 atgccgaaca acccatcgaa caggtgactg cggatattca acaagtaatt agaaactggc
     8821 tgaaataatg tacccttggc tcgaaagcac ttatcaacaa cttaccgatt catttttaca
     8881 gggtcgaggt catcacgcct tactgttcaa aacagatgtt ggtcttggta cagacacctt
     8941 aatctacaag gtggctgaat ggttgctttg cagccaaaaa ctccgccatc aaccttgtct
     9001 tgaatgcaaa agttgcctgc ttatgcaaag caacaaccac cccgatttcc acattcttga
     9061 gccgattgaa gacaaagata ttggtatcga ccaagtgcgt gagttgattt ataaactcca
     9121 gaatttcgcc caacaagggg gaaatacggt cgtttatatt cgaggagttg atcgtttaac
     9181 cgaggcaagc agtaatgccc tgctcaaaac cttggaagag ccacgtgaaa acgtctattt
     9241 cttgctcgaa gcaccgcttc aagcaacagt tttagctacc attcaaagcc gcagccaaac
     9301 ttgggtactt tacgccccac aagcctcaga agtaatggct tggctacaaa cacaatttcc
     9361 ggcaatctca accgacgact tagagattgc actccgcctt tgccactcac gcccactaat
     9421 ttgcaaaaat tttatcgaaa atgaccgctt gcaacagcga aaaacctttt tgcaaacctt
     9481 ctggcgtttc tacaaaagcc acgatgtctt cttgttattc caagccttcg cccaagaaaa
     9541 agagctgatt ttacagcaac tcgaatggct ggaaagtttc tttagcgatg ccctaaaagc
     9601 taaaatggca atcagccacg gttggctaaa cccggatctc caaaacggca ttctcccttt
     9661 tagccaacaa ctaacgaccc acggcttact taaagggcat caaattctcc aacaaaccca
     9721 gcgggattta accgaaatta acgccgtcaa tccggaattg atgttgctag attgcctgac
     9781 gaagttggtt ttggtgtttg agtaaaagcc ctctctcata gaaggtagct ttcaattagc
     9841 cgtaaaaaga ggcttctagc cctagaatat agtttataac ttgcaaaatt atctatcttt
     9901 gttattgtat gaataattca cacaaggaga atattatggc aagaacaacc agcgtgactt
     9961 taggcgaacc tttaaatgat tttgtaaatg aaatggtggc aagtgggcgt tatggctcta
    10021 ccagtgaagt agtgagaacc gctttacgaa tgcttgagga aaaagagcaa tacttagctc
    10081 agttgcgaca agccattgat gatggtgatg ctagtggtta tgcaaccgaa acgattgatg
    10141 aaatgatgtt gagagtaaag gcggaactta atgtataaac tttctgctca agcactccga
    10201 gatcaagaga aactttttcg ttacagtatc tctcaatttg gtgttcaaca aacagaatct
    10261 tattttgcag atctaaagca tattttgaat ttattagctg attttccaac aatgggaact
    10321 tctgcggaat atattcgtgg tggcttgcga atgcacacac atcaatctca tacaatttat
    10381 tatcgaataa aag
//
LOCUS       DANLXS010000081        10365 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.081, whole genome shotgun sequence.
ACCESSION   DANLXS010000081 DANLXS010000000
VERSION     DANLXS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.081"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..753)
                     /locus_tag="PXD71_001495"
     CDS             complement(<1..753)
                     /locus_tag="PXD71_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL6291605.1"
                     /translation="MENFEKQFISLFQRIVPHYRRSEVFYDFITLSALDMYLVTYRDQ
                     AEPNLRERFAHAKARYSDSEFTQLAELLAVTVNALTQKRYDFLGSVFMNLNLGDGYRG
                     QYFTPSHIADLMAKVTLQDCDRIISQQGFVTLSEPTCGSGVMVIGCVNAMFDAKYNPQ
                     QQLWVHCQDVDFTAAMMCYIQLSLLHIPACIVVGDTLLNETKIQMYTLAHLMGNWSHK
                     LEQQIAPLVNSTTEKGCGNNIEKSVIVEDLSQI"
     gene            complement(826..1572)
                     /locus_tag="PXD71_001496"
     CDS             complement(826..1572)
                     /locus_tag="PXD71_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291606.1"
                     /translation="MPNWCENKLQIFCDCIQAKQIIPKLFRKTESDKWCLDFELLVPM
                     PESICIESSSLGDRAMEFLKISPNRRLTRPLLKKYIYGSEIDRIYAKAKHHRWDVGHF
                     INWLIKRPEQQSILWLNLALGQQYLSNLAQHGYKDWYDWSNEQWGCKWNVCSDSCQVS
                     FNDNGSINCLFDTPWGPPDGWFQALCTAFPDIEFILSFFEPGMWFAGKYIANLDGSYC
                     NIYIDDRGIRSFAEEVFNEEFEDEFEEKLE"
     gene            complement(1712..2686)
                     /locus_tag="PXD71_001497"
     CDS             complement(1712..2686)
                     /locus_tag="PXD71_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL6291607.1"
                     /translation="MNTQTKFDLYQHITDRIVAALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEYGYKQSKWITAKKANELGGYIRKGEKATVVMNYRPDK
                     REKLDEEGNIVYDEEGNPEIEQHFFIKKHNLFNLDQCENLPEELYDPISEPKQPTSDP
                     NQYEIFADIRQIIKGMDLHVEMKPSNRAYYQPSADLVVMPEIKQFENAQAFHSVLLHE
                     MTHATGAAKRLDREGITSGKAKFGNKVYAFEELVAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVIKEDKRAIVRASGKAREACQYMLDALNISQLADKNEVFEEAA"
     gene            complement(2931..3338)
                     /locus_tag="PXD71_001498"
     CDS             complement(2931..3338)
                     /locus_tag="PXD71_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6291608.1"
                     /translation="MKHDLDLLKYIWDNRNLNNKAYQYLFHICIEPMRKGDWNRYTIW
                     LNNNGNLVNISAVVYEIVSSQQLKDDIKLWQDMQFHCRPVKVETTGFISFLLHQFFNG
                     ITHVLLEQGMITETEAAEKSYHFIAANSFQRLL"
     gene            complement(3454..4296)
                     /locus_tag="PXD71_001499"
     CDS             complement(3454..4296)
                     /locus_tag="PXD71_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291609.1"
                     /translation="MSGSVLNTYFKHALVKAGFPNCLNIEYSLSHCQGDGVAFYGRLN
                     TETLINLFNKLNPQKRTQKMFSNLLNHIESWEPHYGVDFEIIRNSFGYRYSHCNTMEL
                     SVRTADDLAFFYDRDARKEWYFPTSKVEKYKALWDGFIQDLGQYIKDTSCALESNGYQ
                     IIEASPYQTETIFKFNTENYLIELKRKSSEFYSESVDWLCGDVCDFEAMIEEILQGKL
                     CYADFVATVTDKESGIQLGSDEITGVSYSPTDHSLSGYRQELISNAIASARSNAARFA
                     YLHA"
     gene            complement(4374..4769)
                     /locus_tag="PXD71_001500"
     CDS             complement(4374..4769)
                     /locus_tag="PXD71_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291610.1"
                     /translation="MARKKIQYTETDLHLYSVLWNLRHAHDLFSVKLERETKFGKAFS
                     VFSRFDVCPSYGEVDITYLVVKAGIRKTLRGFAYVGGNGFSHSRHLMSTFMYLVREIL
                     VEHNWISETELYDQPISVYKFRAINFDKW"
     gene            complement(4868..5113)
                     /locus_tag="PXD71_001501"
     CDS             complement(4868..5113)
                     /locus_tag="PXD71_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6291611.1"
                     /translation="MLVHIRLSCIGDVLPTKLNEWTSEHQALQQEISHRALEAWQNRD
                     KDIKSVRFMQQMPSRQGLNFRFLSVSEQNGKLIVSRA"
     gene            complement(5185..5538)
                     /locus_tag="PXD71_001502"
     CDS             complement(5185..5538)
                     /locus_tag="PXD71_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291612.1"
                     /translation="MSQQTAGWDVIPFESSKTVKPSLFWKLGNKLGNIFVSAKHKAKS
                     KYVEVTKGINQRIAEEDEMRALELQMCLDEQAAEYEQEISRIKRKMFFWTLLAAIIAF
                     VCGMAAVFGTQLSLI"
     gene            5912..6355
                     /locus_tag="PXD71_001503"
     CDS             5912..6355
                     /locus_tag="PXD71_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDL6291613.1"
                     /translation="MKVSRFLGYCSIVTGVFSPLAQAQPNIIVYTTKMYPIQNSQLAS
                     QIYYLDQVENFEESISRNLSKSPEIAEQEMRAFFNSADWKAQEQQLKDAYQGVISGWQ
                     NGIKKVPAVFFDNPQSSPSVIYGTTDVQKALDEWHKWVKQQGESK"
     gene            6352..7293
                     /locus_tag="PXD71_001504"
     CDS             6352..7293
                     /locus_tag="PXD71_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDL6291614.1"
                     /translation="MKKTFLTILISASLAVPTTLYPQPARASLNTASIAASSASTSCL
                     EYKVVGTCFWLFCTYFGCKVRTSTKIKHYLPEMVVSSYNHDGQTPWTELQALNPGVKG
                     GEYRSPQRQYSQLTFKNADAFGHPQGAIGQLLGNTGYYCKTQTTPFMPYFLSGLDFFA
                     WRENMPEMFYPEALTPGMREVGRVGDSWGNIYPRSGWVTQAHDYKASATIVQRVADIV
                     SRTAQPHIYFPAAKTGGAGEWPPGPVEEGQARTHKWQILYPQMSNTCSVFPNGGQTDT
                     YSSQISNQGDYAWALWRPYSCCKRRGQTFLYSIDWSH"
     gene            7311..9323
                     /locus_tag="PXD71_001505"
     CDS             7311..9323
                     /locus_tag="PXD71_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL6291615.1"
                     /translation="MKSHFNFKKSHLRITTALLSSVSGLSYADSITDKLEFSKSDSII
                     SDRVFYQIGGGMGYMAPPSRGNTKVVDLGIGWKANLTCGNFDIKTTIKNDLNGLKDGF
                     KDLMGNVIDSAKGAVASMPAMVLQRANPQLYDLLTNGMYQGKLDFNRLKTSCEEMSKV
                     LADHTLGGKWGQAADLESYKQITSSQPDAKQAQKDLEDKKGKDGRVWIGGQKKGGENQ
                     EAINVVGDVVKAGFNLLNKRNALDTSTISGSSCDGNLCQTWDNPQTAADFLTKIIGEQ
                     SISTCKEDCGTKTSSKAGVGLSPEIEEEHVRTIVKLENVLNMDVPKAELLADLSSTTV
                     PVTRGLIESLRDDPDVEVLSQRLSAEIATSKIMEKMLLARRAVLAGMREPNVAQNADA
                     QVELERVLNIIDREISQVKLEMDMQKMITGNTASVILQSKSEREALAGAHSLSQDSLD
                     KRVQGLENGNVALTDLEAGSNINLSRRNIALSIPNIPNVAALIPNLPNRGGSGVGGNF
                     PSLAQISGSTMDQATGLLRNFEGFISKAQWDVNAHRVGYGSDTITRADGTIVKVTKDT
                     VITKEDAERDLARRTAIFANNVRKELGDSNWNALPPNAQAVLVSYAYNYGSLAKTKSV
                     LDAARRSAQSGDMNALATAIRNRQVDNNGINARRRNQEADYILGKN"
     gene            9344..9772
                     /locus_tag="PXD71_001506"
     CDS             9344..9772
                     /locus_tag="PXD71_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291616.1"
                     /translation="MRKLLGITVLTLCTTSAFATDKNVVFSCTSTEGKPLTVKRVGND
                     YEYSYDKTIFKNPIKKAVTNDGSIIARGSGFTTYALELKNDGLKYVVGFVQPNGNSKE
                     FIEPGATISRDNDQPSIGSVDCDPYKKIYYKFDVHLMNTL"
     gene            9988..>10365
                     /locus_tag="PXD71_001507"
     CDS             9988..>10365
                     /locus_tag="PXD71_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291617.1"
                     /translation="MDITTQNIELRYLSEQFYLDVAPHLKEILLKETRPYCVLLVRVK
                     NLDFALPLRSNLPEREGVGIKTIPNPQEYGKFKGIDFSKAILLTDKSYLKTDTVQLKN
                     KSELLNIQGNERRIVREFKKYVSE"
BASE COUNT         3281 a         2025 c         1995 g         3064 t
ORIGIN      
        1 gatttgagat aaatcttcca ctatcactga tttttctata ttgttaccac agcctttctc
       61 agtagtgcta tttacaagtg gtgcaatttg ttgctccagt ttatgagacc aatttcccat
      121 caaatgagcc agcgtataca tttgaatttt tgtttcattc aatagcgtat caccaactac
      181 aatacaagca ggaatatgta ataacgatag ctggatataa cacatcatgg cagctgtaaa
      241 atcaacatct tgacaatgaa cccaaagttg ttgttgggga ttgtatttcg catcaaacat
      301 cgcattaaca caaccaatca ccattacacc cgaaccacaa gttggttctg ataaagtaac
      361 aaatccctgt tgtgagatga ttctatcaca gtcctgtagt gtaactttcg ccattagatc
      421 tgcgatgtga gatggtgtaa agtattgccc tctataacca tcaccaagat tgagattcat
      481 aaatactgag cctaagaaat catagcgttt ttgtgttaaa gcattcaccg taacagctaa
      541 caattctgct aattgagtaa actcagaatc tgaatatcgt gccttagcat gagcaaagcg
      601 ttctcgcaaa ttaggttctg cttgatctcg atacgttact aggtacatat caagtgcaga
      661 gagtgtaata aaatcataaa acacttctga acgtcgataa tgtggtacga tacgttgaaa
      721 taagctgata aattgctttt caaagttttc cattagacta ctccttaaag tttacggagt
      781 aatcccccta gggaattacc cctagggtgg aaaaattaat taaggctatt caagtttctc
      841 ttcgaactca tcttcaaatt cttcattaaa gacttcctca gcaaaactgc gaatacccct
      901 atcatctatg taaatattgc agtatgagcc atcaagattt gcgatatatt tgcccgcaaa
      961 ccacattccc ggctcaaaaa atgaaagtat aaactcaatg tcaggaaagg cagtacaaag
     1021 tgcttggaac caaccatcag gtggtcccca tggcgtatca aataggcaat taatagatcc
     1081 attatcatta aagctaactt gacagctatc tgaacataca ttccatttac agccccactg
     1141 ttcatttgac cagtcatacc aatctttgta accatgttga gctaggttag acagatattg
     1201 ctggcctaat gcgaggttta gccataatat actttgttgt tcaggacgtt ttatcaacca
     1261 gttgataaaa tgacctacat cccagcgatg atgctttgcc ttagcatata tccgatcaat
     1321 ttcggaccca tagatatact ttttgagtag agggcgtgtt aaacgtctat ttggtgaaat
     1381 ttttagaaat tccattgctc tatcaccaag actacttgat tcaatgcaga ttgattctgg
     1441 cattggaacg agtaattcaa agtctaaaca ccatttatca ctttcagttt ttctgaaaag
     1501 cttaggaata atttgtttag cttggataca atcgcaaaaa atttgtagtt tattttcaca
     1561 ccagtttggc ataatgtact cctaaataaa gtaggggtac acttgccttg aggcaatgta
     1621 ccccaagggt taaaagataa tcgatacata attcctaaag gattatgtat tactatgaaa
     1681 gattgtgttg aaagtacctt tcagcaaaga gttaagctgc ttcttcaaag acctcgtttt
     1741 tatcagccag ctgtgagata tttaatgcat ctaacatata ttgacaagct tcacgagctt
     1801 taccagaagc ccggacaata gcccgtttat cttcttttat aactcttatc caactttcaa
     1861 tatatgccgc attttgaggt accgtatcaa atccaaggta ggcacacaaa aatgctcccc
     1921 ccatttcggc aaccaactcc tcaaaagcat agactttgtt tccaaatttt gcttttccgg
     1981 aggtaatacc ctcacgatcg agccttttag cagcaccagt tgcatgagtc atttcgtgca
     2041 ataacacact atgaaacgct tgagcatttt caaactgttt tatttccggc attacaacta
     2101 aatctgcact gggttgataa tatgcccgat tactcggttt catttccacg tgtaaatcca
     2161 ttcctttgat aatctgccga atatcagcaa aaatctcgta ttggtttgga tccgatgtag
     2221 gctgtttagg ttctgatata ggatcgtaca gttcctcggg taaattttca cactggtcta
     2281 gattgaacag attgtgcttc ttaatgaaaa agtgttgttc aatttctgga ttaccttcct
     2341 cgtcatacac aatatttccc tcctcatcaa gtttttctct cttatcagga cggtagttca
     2401 ttacaacagt agctttttca cctttacgga tatatccgcc gagctcgtta gctttttttg
     2461 ctgtaatcca cttactctgt ttgtaaccgt attctgcggt agcaatccag agtaatagtg
     2521 tgttgatacc agaatacaat cggttagaga ccgcattacg aggtaatgcc atattacatt
     2581 ctggatgact ccacggtttt aaccatggtg cagtcccctg ttccagtgct gcaacaattc
     2641 ggtctgtgat atgttggtat aaatcaaatt tagtttgagt gttcattagt gtttctccaa
     2701 agttcaggta gaaaatgggg aaacactatt acccttatcg ggcactagtt tccccgatgg
     2761 gtggtttaaa atctcaacag agatagtttt tctaggttca tcaaaaccag tagaactgcc
     2821 tataatgcag taactacaga taatttgtta atcagtattg attcaagtta aacataatcc
     2881 tcatatcgaa aattactttg gtaatacaaa gttacattat ttcagatcca ttaaagtaaa
     2941 cgttgaaagc tattagcagc aataaagtga taactttttt ccgcagcttc tgtttccgta
     3001 atcattccct gctccagcaa aacatgggtg attccattga aaaactgatg taatagaaat
     3061 gaaataaaac ctgttgtttc aacttttact gggcgacaat gaaactgcat atcctgccaa
     3121 agtttaatat catcttttaa ctgttgagag ctcacaattt catatacaac agcagaaata
     3181 ttaactaaat taccgttgtt attcagccaa attgtataac gattccaatc tccttttctc
     3241 attggctcta tacaaatgtg aaataggtat tggtaggctt tattatttaa attgcggtta
     3301 tcccagatgt atttgagtaa atctaaatcg tgtttcataa tgtttctcca aaagaaaaaa
     3361 gtggagaaac atcttaccta tagggaaaat atttccccac agggtaaaaa acaatctcga
     3421 taagcggtca aattgaaata aaaaacctac aaatcaggca tgcaagtagg caaaacgtgc
     3481 ggcattacta cgagcagaag ctatagcatt actaataagc tcttgtcgat aaccactcaa
     3541 tgaatggtct gtcggcgaat agcttacgcc tgtaatttca tcgctaccta gttgaatgcc
     3601 actttcctta tcagtaacgg ttgctacaaa atcagcatag caaagtttcc cttgtaggat
     3661 ttcctctatc attgcctcaa aatcgcagac atcaccacat agccaatcaa cagactctga
     3721 ataaaattca gaggatttac gtttaagttc tatcagatag ttttccgtgt taaatttgaa
     3781 aatagtttcc gtctgatacg gtgaggcctc aataatctga tagccattcg attctaaggc
     3841 acaggaagta tctttaatat actgacctaa atcttgaata aatccatccc acagggcttt
     3901 gtatttttcc acttttgatg tagggaaata ccactctttt cgggcatcac gatcatagaa
     3961 aaatgccaaa tcatctgctg ttctaacaga aagctccatc gtattacaat gcgaatagcg
     4021 gtacccaaaa ctatttcgta tgatttcaaa atccacgcca tagtgaggct cccaagattc
     4081 aatgtgattc agcagattag aaaacatttt ttgtgtgcgt ttctgaggat ttagcttgtt
     4141 gaaaagatta attaacgtct ccgtattaag cctaccgtag aatgctacgc catcgccttg
     4201 acaatgagaa agtgaatact caatgtttaa gcaattagga aaaccagctt taacaagagc
     4261 gtgtttaaaa taagtgttta agactgagcc agacattgcg cttctcctaa aattagaggg
     4321 aaacacatta cccttttccg gaaattgtgt ttcccgaaag ggtgaaagat tgattaccac
     4381 ttatcgaagt taatcgctct gaatttataa acggatatag gttgatcata gagttctgtt
     4441 tccgaaatcc aattgtgttc aaccaagatt tctctgacaa gatacataaa tgtactcatc
     4501 aaatgacgac tatgactaaa cccgtttcca ccaacataag caaaccctct cagggttttg
     4561 cgaattcctg ctttaacaac aaggtaagta atatcaacct cgccataaga cgggcagaca
     4621 tcaaagcgag aaaaaacaga aaatgctttg ccaaacttgg tctcacgctc taatttcaca
     4681 ctgaatagat catgtgcatg gcgtaaattc caaagtacac tgtataagtg caaatctgtt
     4741 tcggtatatt gaattttttt acgagccata atgtttctcc taaaaaataa aagggaaaca
     4801 ttacttccct ctggggataa tgcctcccct tgtgaggtat aagaatagga tgtgaatatg
     4861 aagttgccta tgctcgacta acaattagct tgccgttctg ctcggacaca ctcagaaagc
     4921 gaaaattaag tccttgccga gacggcattt gttgcataaa tcgcacgctt ttgatgtcct
     4981 tatctcgatt ttgccacgct tctaaagctc tatgactaat ttcttgctgt aaggcttgat
     5041 gctctgatgt ccattcatta agtttagttg gtaaaacatc tccaatacag gaaagtctaa
     5101 tatgaactaa catacatttc tccagaataa ggggaaatgc attcccctta agggaaacaa
     5161 catttccccg atgggtgagt taaattagat taaagagagc tgagtaccaa aaacggcagc
     5221 cataccacat acaaaggcaa taatggcagc caacaaagtc cagaaaaaca tcttacgctt
     5281 aatgcgggaa atttcctgtt catattcggc tgcttgttcg tctaagcaca tttgcagttc
     5341 taaggctcgc atttcatctt cttcagcaat ccgttgattg atgcctttgg tgacttcaac
     5401 atacttgctt ttggctttat gtttcgctga aacaaaaata ttacctagct tgttgccaag
     5461 tttccaaaaa agagaaggtt ttacggtttt ggatgattca aacgggataa catcccatcc
     5521 tgcggtttgt tgactcatag cgagttctcc taaaagttaa gtgaataaaa ttcctcagga
     5581 gatcgcaatt ccaagggaat tgaaatatcc ctcggaaggt aggtgtgtag gtagcattcc
     5641 tgcaaatttg cagagttccc tttcagaatg ctgggagaca ttttgggtaa ataactcaac
     5701 tacctctaag gatagccgcc gcttttcaag agttatgcga cttttaaggg ctgtatctac
     5761 agcgtcttaa tgcttaatat catacgaatt cctccaataa aatcaaccgt ttttttgttc
     5821 aaaaaatatc tcttttttga aaagagtttt tcctatcctc aacataaaaa tctcgctaat
     5881 tttctgctta tttgttcggg aggagagcgt gatgaaagta agtagatttc tgggatattg
     5941 cagtatagta acaggtgtgt tttcgcccct tgcacaagca caacccaata tcatcgtcta
     6001 taccacaaag atgtacccta ttcaaaattc acaacttgct tcccagattt attatcttga
     6061 ccaagtagag aattttgaag agtcaatcag tcgaaacctc agtaaatcac cagagattgc
     6121 cgaacaagaa atgcgtgcat tctttaattc agcagattgg aaagcccaag agcaacaatt
     6181 aaaagacgct tatcaaggcg tgatcagtgg atggcaaaat ggcattaaga aagtacctgc
     6241 ggtgtttttt gataatccac aatcttctcc gagtgtgatt tatggtacga ctgacgtaca
     6301 gaaagcatta gatgaatggc ataaatgggt taaacaacaa ggtgaatcaa aatgaaaaaa
     6361 acgtttctta cgatactgat ctctgccagt ttagctgtcc caacaacctt atatcctcaa
     6421 ccagccagag cctcattgaa taccgcttcc attgctgcat caagtgcctc aacaagctgt
     6481 ttggaatata aagttgttgg aacctgtttt tggttatttt gtacctactt tggatgtaag
     6541 gttcgcacat caaccaaaat taagcattat ttacctgaaa tggttgtttc atcttataac
     6601 cacgatgggc aaacgccatg gacagaatta caagctctca atcctggtgt aaaaggtgga
     6661 gaataccgtt ctccacaacg ccaatactct caactcactt tcaaaaatgc ggatgcgttt
     6721 ggacacccac aaggagctat cggtcaatta ttaggaaata cggggtacta ttgtaaaact
     6781 caaactacgc catttatgcc ttactttttg agtgggctgg atttttttgc ctggcgtgaa
     6841 aatatgccgg aaatgtttta cccggaagct ctgactccgg gaatgcggga agttggccgt
     6901 gtaggcgata gctggggaaa tatttaccca cgttccggtt gggtaaccca agcacacgat
     6961 tataaagcct ctgcgaccat tgtgcagaga gtggcagaca ttgtgagccg aacagctcag
     7021 ccacacattt atttcccagc agccaaaaca ggtggtgcag gagaatggcc tccaggaccg
     7081 gtcgaagagg gacaagcaag aacacataaa tggcaaatac tttaccctca aatgtcaaat
     7141 acctgctctg tttttcctaa tggagggcaa acagatacct attcttctca aatttcaaat
     7201 caaggtgatt atgcttgggc attatggcga ccttatagtt gttgcaaacg ccgtgggcaa
     7261 acattccttt acagtattga ttggtcacac taataacatt gggagcaata atgaaatctc
     7321 actttaactt caaaaaatcc cacttacgga tcacgacagc attattatca agtgtatcag
     7381 gactatctta tgccgattcg attacggata aactggaatt tagtaagtcg gattcgatta
     7441 tttcagaccg tgttttttac caaatcggcg gtggaatggg ttatatggct ccacctagtc
     7501 gtggtaatac caaagttgtt gatttaggaa ttggttggaa agcgaattta acctgtggta
     7561 attttgatat taaaacgacc attaaaaatg atttaaacgg tctcaaagac ggctttaaag
     7621 atttaatggg gaatgtcatt gattcagcga aaggtgcggt tgcaagtatg cctgcaatgg
     7681 tattgcaacg ggccaaccct caactctacg atttactcac aaatggtatg taccaaggta
     7741 agctggattt caaccgtctt aaaaccagtt gtgaagaaat gtccaaagtt ttagcagatc
     7801 atacactggg aggaaaatgg gggcaagcag ctgatttaga aagctataag caaattacct
     7861 cctctcaacc tgatgcaaaa caagctcaaa aagacctgga agataaaaaa gggaaagatg
     7921 ggcgagtttg gataggaggt cagaaaaaag gtggggaaaa ccaagaagct atcaatgttg
     7981 ttggcgatgt tgtcaaagct gggtttaatt tactgaacaa acgtaatgcc ttagatacca
     8041 gtacaatttc aggttcaagc tgtgatggga atctttgcca aacttgggat aatccacaaa
     8101 cagcagccga ttttttaact aagattattg gtgaacaatc tatttcgact tgtaaagagg
     8161 attgtggcac taaaaccagc tcaaaagccg gtgtaggctt gagtccggaa attgaggaag
     8221 aacatgttcg tactattgtc aaattagaga atgtactgaa tatggatgtg cctaaggctg
     8281 aattgttagc tgatttgagt tcaactactg taccagtaac aagagggctt attgagagtc
     8341 ttcgagatga tcctgatgtt gaggtattaa gccaacgctt atctgcagaa attgcgacta
     8401 gtaagataat ggaaaaaatg ctcctggctc ggcgagcagt cttagctggt atgcgtgagc
     8461 caaatgtcgc tcaaaatgcg gatgcacaag tagaactgga aagagtactc aatattattg
     8521 accgtgaaat ttcccaagta aaactagaaa tggatatgca aaaaatgatc accggcaata
     8581 cggcttcggt gattttgcaa agcaaatccg aacgagaagc tcttgctggt gctcatagtt
     8641 tatctcaaga cagtctggat aaacgtgttc aaggacttga aaacggtaat gttgcattaa
     8701 ctgatttaga ggcaggctct aatattaacc tctctcgtag aaatattgca ttgagtatcc
     8761 cgaatattcc gaatgtagct gctctcatac ctaatctgcc aaatcgaggt ggttcaggtg
     8821 taggtggaaa tttcccatca ctcgcacaaa tcagcggttc aacgatggat caagcaacag
     8881 gattactacg taattttgaa ggctttatct ctaaggctca atgggatgta aatgctcatc
     8941 gagtaggata tggttccgat acaattaccc gagctgatgg tacgatcgta aaagtaacca
     9001 aagatacggt cattaccaaa gaagatgcag agcgtgattt ggcccgccga acagctatct
     9061 tcgcaaataa tgtgagaaaa gagcttggag attcaaattg gaatgcgtta ccgccgaacg
     9121 ctcaagctgt tttagtgtct tatgcatata attatggttc acttgctaaa acaaaaagcg
     9181 tacttgatgc agccagaaga tcagctcaaa gtggtgatat gaatgcttta gctactgcaa
     9241 tcagaaatcg ccaagttgat aataatggca tcaatgcacg tcgccggaat caggaagctg
     9301 actatattct tggtaaaaat taacctcata aggagttaca tttatgcgta aattattagg
     9361 tattactgta cttacacttt gtacaacttc agcatttgca acggataaaa atgtggtatt
     9421 ttcttgtacc agcacagaag gtaaacctct cacagttaaa cgggtaggaa atgattacga
     9481 gtattcgtat gataaaacta tcttcaaaaa cccaatcaaa aaagcggtga ccaacgatgg
     9541 ctcaattatc gctagaggat caggttttac cacgtatgca ttagaactga aaaatgatgg
     9601 tttgaagtat gtagttgggt ttgttcaacc aaatggtaac tcaaaagagt ttattgagcc
     9661 tggagcaacg attagccgtg ataatgatca accaagcatt ggctctgtag attgtgatcc
     9721 ttacaaaaaa atctattata agtttgatgt tcatttaatg aataccttgt aatgcgataa
     9781 ttcaataata tgggggaatg gcttgatttt tcatccccca tactctatta tatagatgtg
     9841 gtgctagagc cagcccacgt taaaaaatac aaagctcggt agatgaacta ttgtggtgcc
     9901 agagccggcc cacgttaaaa aatacaaagc tcggtagatg aggcccctta agtcgtaaga
     9961 tttaaggggt tttgcttagg atataaaatg gatattacaa cacaaaatat tgaacttaga
    10021 tacttatctg agcagtttta ccttgatgtc gctccacatc tgaaagaaat tttgcttaaa
    10081 gagactcgcc catactgtgt tctattagtt agagttaaaa atttagattt tgccctacca
    10141 ttgcgttcta atttacctga aagagaagga gttggaatta aaacgatacc taatcctcag
    10201 gaatacggca aatttaaagg aattgatttc tcaaaagcca ttctactgac agataaaagc
    10261 tatttaaaaa cagataccgt tcagctcaaa aacaaatcag aattgttgaa tatccaaggt
    10321 aatgagcgta gaattgttcg tgaatttaag aaatatgtga gtgaa
//
LOCUS       DANLXS010000082        10170 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.082, whole genome shotgun sequence.
ACCESSION   DANLXS010000082 DANLXS010000000
VERSION     DANLXS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.082"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            156..470
                     /locus_tag="PXD71_001508"
     CDS             156..470
                     /locus_tag="PXD71_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDL6291618.1"
                     /translation="MKKLTPDEAIDIAYDIFLEMAGENLDPADILLFNLQFEERGAVE
                     MVETSEDWDQEIGTLIDPDAFAEVWVGLVNEQDEMDDVFARFLISHDEENREYHVVWK
                     EK"
     gene            483..995
                     /gene="hpaC"
                     /locus_tag="PXD71_001509"
     CDS             483..995
                     /gene="hpaC"
                     /locus_tag="PXD71_001509"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HDL6291619.1"
                     /translation="MANIDTNQPIEASLKFREAMAHLSSAVSIVTTNGVAGKAGLTVS
                     SVCSVTDSPPTLLFCINKNSHVHDIIQQNGKVCVNVLSHEQEDLAKHFAAILESTMEE
                     RFGWDIWNNGFDDQPVLRSAISALQGNIVDTHAVGTHTIFVVEVSHIDVEPNHSLVYF
                     ARQFRSVNID"
     gene            1113..1985
                     /gene="tehB"
                     /locus_tag="PXD71_001510"
     CDS             1113..1985
                     /gene="tehB"
                     /locus_tag="PXD71_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HDL6291620.1"
                     /translation="MENLICYKQMPVWTKATLPQMFQEKHNTKVGTWAKLTILKGSLK
                     YYELTEEGEIIKEDIFDANHQRPMVEPQLWHKVEALSDDLECQLAFYCKPEDYFAKKY
                     NLTATHSEVLNAVNYTKGGKALDLGCGRGRNALYLNLLGFDVTAVDHNQESIDFLNYM
                     IEKEQAQNITTAVYDINEATIGSQNGEYDLIISTVVMMFLNRERIPAIIENMQKNTKV
                     GGYNLIVCAMSTDDYPCPMPFSFTFKEGELADYYQGWELVKYNEDLGHLHKTDENGNR
                     IQLRFATMLAKKIS"
     gene            2123..3160
                     /gene="purM"
                     /locus_tag="PXD71_001511"
     CDS             2123..3160
                     /gene="purM"
                     /locus_tag="PXD71_001511"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL6291621.1"
                     /translation="MSNTQLSYKDAGVDIHAGNELVERIKPDVKRTRRPEVMGGLGGF
                     GALCALPTKYKEPILVSGTDGVGTKLRLAIDLNKHDTIGQDLVAMCVNDLVVQGAEPL
                     FFLDYYATGKLEVDVATNVVKGIADGCVISGCALVGGETAEMPGMYHEGDYDLAGFCV
                     GVVEKSKIIDGSTVKTGDALIALASSGPHSNGYSLIRKVLEVSRSNPATDLLEDKPLS
                     EHLLAPTKIYVKSILKLVEEVDVHAIAHLTGGGFWENIPRVLPESAKAVIDESSWQWP
                     AIFSWLQEKGNISRYEMYRTFNCGVGMVIALPERDVEKALTLLKESGENAWLIGKIED
                     LGTGSEQVEIR"
     gene            3238..3516
                     /locus_tag="PXD71_001512"
     CDS             3238..3516
                     /locus_tag="PXD71_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL6291622.1"
                     /translation="MQLFWPIYFVLMNLAALYLMYSDKQKAIKKEWRVPEITLLSFCL
                     LGGAIGVYLGMKYFRHKTQHWYFHVAVVLSTFAWLIGLPYFYLALSSY"
     gene            3659..5086
                     /gene="aspA"
                     /locus_tag="PXD71_001513"
     CDS             3659..5086
                     /gene="aspA"
                     /locus_tag="PXD71_001513"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDL6291623.1"
                     /translation="MSDVRIEHDLLGEREVPNSAYWGIHTLRAIENFNISNVTISDVP
                     EFVRGMVMVKKATALANGELGAIPKKIAKAIVAACDEVLVKGRCLDQFPSDVYQGGAG
                     TSVNMNTNEVIANLALEILGEAKGKYDIVNPMDHVNASQSTNDAYPTGFRIAVYNSIT
                     KLINKITYLQTAFENKATEFSDVLKMGRTQLQDAVPMTLGQEFKAFAVLLDEEVRNLK
                     RTAELLLEVNLGATAIGTGLNTPEGYSTLAVKYLAEVTQLPCSLSENLIEATSDCGAY
                     VMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEV
                     VNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIELLSNACINLRDKCIDG
                     ITANRDICQNYVFSSIGIVTYLNPFIGHHNGDIVGKICATTGKSVREVVLERGLLTEE
                     QLDDILSIENLMNPTYKAKRFTEEE"
     gene            complement(5161..7179)
                     /gene="rep"
                     /locus_tag="PXD71_001514"
     CDS             complement(5161..7179)
                     /gene="rep"
                     /locus_tag="PXD71_001514"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDL6291624.1"
                     /translation="MKLNSQQQQAVEYTKGACLVLAGAGSGKTRVIINKIAHLIAHCG
                     YSPKQIAAVTFTNKAAREMRERVDHSIGKENSKGLTISTFHTLGFEILKREYKLLGFK
                     SGMTLFDEQDQIALLKHLLPENVTEDKDLLKALIATISNWKNDLLSPERVLTRVRDER
                     ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKSRWQQKIRYLLVDEYQD
                     TNTSQYELIKLIVGNEPNFTVVGDDDQSIYSWRGAKPENMQRLREDFDLQVIKLEQNY
                     RSSQRILHCANILIDNNDHLFSKRLFSELGQGEKLQVIEAKNEEHEAERIVGELIAHR
                     FSKKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLV
                     VNQDDDAAFLRIVNTPKREIGAATLEKLGILANEKQVSLFEAIFDFELIQRLTPKPYQ
                     ALQDFARWIVEIADQADRSDPIEAVKDLLAKIQYEAYLYEMAANPKVAEMQSRNVQTL
                     FEWVQAMLEGDEIEPPMNLVQVVNRLTLRDMLERGEEDDEADQVQLMTLHASKGLEFP
                     HVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKP
                     EPSRFLLELPQDDLEWESEKPRMTEQEKEAKSSANIANLMAMLKANKK"
     gene            7427..8542
                     /gene="potA"
                     /locus_tag="PXD71_001515"
     CDS             7427..8542
                     /gene="potA"
                     /locus_tag="PXD71_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDL6291625.1"
                     /translation="MEKVEQKAIIELKNVTKSYGSKTILKNLNLTINDGEFLTILGPS
                     GCGKTTALRLIAGFEDLTEGSIILDGQDVSNVPAEQRPVNTVFQSYALFPHMTIFENV
                     AFGLRMQKVPNAEIEPRVMEALRMVRLEDRAQQKPAQLSGGQQQRIAIARAVVNKPKV
                     LLLDESLSALDYKLRKEMQNELKALQRQLGITFIFVTHDQEEALTMSDRIIVMNAGKI
                     AQDGTPREIYEEPSNLFVARFIGEINVFDATVIERVDATNVKANVEGRICNIKVEDLE
                     VQPNQKLKVLLRPEDIVIEELDENESSKAIIGHIIDRNYKGMTLESSVRLDHNGMNVL
                     VSEFFNEDDPNIDHEVGQKVALTWYEGWEVVLNDEGE"
     gene            8529..9392
                     /gene="potB"
                     /locus_tag="PXD71_001516"
     CDS             8529..9392
                     /gene="potB"
                     /locus_tag="PXD71_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDL6291626.1"
                     /translation="MKANNKFQNGAVATIFGWLLLFVLVPNVLVLIISFLTENRQSQY
                     FVDFAFSFDAYKSLFNDTYAAVLWNSLYMAGIATFFCLIIGYPFAFFIAKMPERIRPF
                     LLFLVVLPFWTNSLIRIYGIKIFLGVKGFLNESLMALGIISEPMRLLNSEIAIIIGLV
                     YILLPFMILPLYSSIEKIDNRLLEAAKDLGANAFQRFIKVTIPLTMPGIVAGCLLVLL
                     PAMGMFYVADLLGGGKTPLVGNIIKSLFLNTNQFALGSAVSIALTILMALMLYVYYRA
                     NKLLNKKVELE"
     gene            9392..10159
                     /gene="potC"
                     /locus_tag="PXD71_001517"
     CDS             9392..10159
                     /gene="potC"
                     /locus_tag="PXD71_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDL6291627.1"
                     /translation="MKKMFRNLFMLVVFAYLYIPIIILVINSFNEDRFGLNWKGFTWN
                     WYERLFSNDTLVQATLNSLTIAFFAATFSTILGALTSIALYRYRFRGKKLVGGMLFVV
                     MMSPDIVMAVSMLVLFMILGIKLGFISLLIAHITFCLPYVVIAISSRLSDFDGKMLEA
                     AKDLGASEVTILRKIILPLALPSIISGWLLSFTISLDDVIVSSFVTSPSYEVLPLKIF
                     SLVKTGVTPEVNALATIMIIFSLTLVVLSQLVVKKKV"
BASE COUNT         3020 a         2008 c         2049 g         3093 t
ORIGIN      
        1 ctaccgcaag gctggcaaat tttaaaggaa aaagcagccg gaatggtggt aagccgtctg
       61 attcagctat aatctttttg ttttttataa ctaaaaaata taaaataaaa cttatctatc
      121 taacatttta ttaacactca aattagaata acgaaatgaa aaaattaacc cctgatgaag
      181 ccattgatat tgcctacgat attttcttag aaatggcagg cgaaaattta gatcctgccg
      241 atattttact gttcaacctc caatttgaag agcgtggtgc agtggaaatg gtcgaaactt
      301 ctgaagactg ggatcaagaa atcgggactt taattgaccc tgatgccttt gccgaagttt
      361 gggttggctt agtcaatgaa caagatgaaa tggacgatgt attcgcccgt ttcttaattt
      421 cccacgatga agaaaaccgt gaatatcacg ttgtttggaa agaaaaataa aaaggagaaa
      481 aaatggctaa tatcgacact aatcaaccaa tagaagcctc gcttaaattt cgtgaagcaa
      541 tggctcactt atcttctgcg gtgagcattg tgacaaccaa tggcgtagcc ggcaaagcag
      601 ggctgacggt ttcgtctgtt tgttccgtta ccgattcccc accaacgctc ttattttgca
      661 ttaataaaaa cagccacgtt cacgatatta tccaacaaaa cggcaaagta tgcgttaatg
      721 tgttaagcca cgaacaagaa gacttagcaa aacattttgc cgccatttta gaaagtacaa
      781 tggaagaacg ctttggttgg gatatttgga acaacggctt tgacgatcag cctgtgttac
      841 gcagtgctat ttctgcttta caaggcaata ttgttgatac gcacgcagtg ggtacgcata
      901 ctatttttgt ggtagaagtg agccatattg acgtagagcc gaaccacagt ttagtttatt
      961 ttgcccgcca atttagaagc gtgaacattg actaaaatat atttaacaga aatttgatca
     1021 aatccgactt ttttagtcgg attttttatt tcctgttttt aaaacagtgc taaaattttg
     1081 ccgtttatgt ttgactgaca aaaggaaaca aaatggaaaa tttaatttgc tacaaacaaa
     1141 tgccggtgtg gacaaaagca accttaccac aaatgttcca agaaaagcac aacaccaaag
     1201 tcggaacttg ggcaaaactc accattctca aaggttctct caaatattat gaattaaccg
     1261 aagagggaga aattatcaag gaagatattt ttgatgcaaa ccaccaacgc ccaatggttg
     1321 agcctcaact ttggcataag gttgaagctc tttctgacga tttagaatgc cagttggctt
     1381 tctattgcaa gccggaagat tattttgcta aaaaatataa tctgaccgct acccattcag
     1441 aagtgctaaa tgccgtcaat tataccaaag gtggcaaagc gttagattta ggttgcgggc
     1501 gaggacgcaa tgcactttat ctgaatttac tgggttttga tgtcactgct gttgatcata
     1561 atcaagaaag cattgatttt ctcaactata tgatcgaaaa agaacaagct caaaacatta
     1621 ctaccgctgt ttatgatatt aacgaggcaa ctatcggctc gcaaaacggt gaatatgacc
     1681 tgattatttc cactgttgta atgatgttct taaaccgtga acgcattcct gccattattg
     1741 agaatatgca gaaaaacact aaagtcggcg gttataactt aatcgtatgt gcaatgagta
     1801 ccgatgacta cccttgcccg atgccgttct cctttacctt caaagaaggc gagctcgccg
     1861 attattatca aggttgggaa ttagtcaaat ataacgaaga tttaggacat ttacacaaaa
     1921 ctgatgaaaa cggcaaccgc attcaactac gttttgcgac aatgttggcg aaaaaaatca
     1981 gctaaactta accgcttgca gggggaatta gcttccccct aattcatcaa atccattcta
     2041 tttattttca ctttttcatt ccgcaaacgt ttgcttgtgc tataatggct tcgtttttta
     2101 gtcatttact gttgaggtta aagtgagcaa cacacaacta agttacaaag atgccggcgt
     2161 tgatattcac gcaggaaacg agttagtcga acgaatcaag cctgatgtta aacgcacccg
     2221 tcgccctgaa gtaatgggcg gattaggtgg ttttggtgct ttatgtgctt taccgacaaa
     2281 atataaagag ccgattttag tttccggtac cgatggcgtc ggcacaaaat tacgcttagc
     2341 cattgactta aacaaacacg atactatcgg gcaagatttg gtagcgatgt gtgtgaatga
     2401 ccttgtcgtg caaggtgctg aaccgctttt cttcttagat tattacgcca ccggtaaatt
     2461 agaggttgat gtagccacca atgtcgtcaa aggcattgca gacggttgcg tgatttcagg
     2521 ttgtgcttta gtgggaggtg aaacggctga aatgccggga atgtaccacg aaggcgatta
     2581 cgatttagcc ggattctgtg tcggtgtggt agaaaaatct aaaatcattg acggttcaac
     2641 ggtgaaaacc ggtgatgcct taattgcatt agcgtccagt ggcccgcatt ccaacggcta
     2701 ttcgttaatt cgtaaagtat tggaagtcag ccgatccaat cctgcaaccg atttattgga
     2761 agacaaaccc ttaagcgaac acttattagc cccaactaaa atttatgtaa aatcaatcct
     2821 aaaattagtt gaagaagttg atgttcacgc tatcgctcac ttaaccggtg gcggtttctg
     2881 ggaaaatatt ccacgcgtat tgccggaatc tgccaaagcg gtgattgatg aatcgagctg
     2941 gcaatggccg gcgatattta gctggttaca ggaaaaaggc aatatcagcc gttatgaaat
     3001 gtaccgtact ttcaactgcg gtgtcggaat ggtgatagcc ttacctgaga gagatgtcga
     3061 aaaagccctt actcttctta aagagagtgg tgaaaatgcg tggcttatcg gtaaaattga
     3121 agacttaggc accggtagcg agcaagttga aatccgctaa tttgcaaaat aaatgctaaa
     3181 attgaccgct tgtagtaact gatctgcaaa cggttaaatt tttattgagg aatgcaaatg
     3241 caactatttt ggcctatcta ctttgtctta atgaacctag ctgccttata tttaatgtat
     3301 agtgacaaac aaaaagcgat caaaaaagaa tggcgagtgc cagaaattac tctcctctct
     3361 ttttgcctct taggtggggc tatcggtgtt tacctaggta tgaaatactt tcgccataaa
     3421 acccaacatt ggtatttcca cgtcgctgtt gtgctttcta cttttgcttg gctgattggc
     3481 ttgccttact tttatttagc tttatcatca tattaattta tcattacttt ttttaatccg
     3541 aaagcccaat ttatatccta gtccgctcac aaaaaatttg atttagatcg cattatttgt
     3601 ctattaaaat cgttattatc taccaaaatc gattgtcatt tattttaagg agatgaatat
     3661 gtcagatgtg cgtattgaac acgatttact tggtgaaaga gaagtaccta atagtgctta
     3721 ttggggcatt catacattaa gagcgattga gaattttaat atctcaaatg tcaccatttc
     3781 tgatgtacct gaatttgtgc gtggcatggt aatggtaaaa aaagctactg cgttggcaaa
     3841 tggtgaattg ggtgcaatcc cgaaaaaaat tgccaaagct attgtggcag cctgtgatga
     3901 agtattagtg aaaggacgct gtctcgatca attcccttca gatgtttatc aaggtggagc
     3961 cggcacatcg gtgaatatga ataccaatga agtgattgcc aaccttgcat tagaaatttt
     4021 gggcgaggca aaaggtaaat atgatattgt caatccaatg gatcacgtta atgcaagcca
     4081 atccaccaat gatgcctatc caaccggttt ccgcatcgcg gtttataaca gtatcactaa
     4141 attaatcaat aaaatcactt acctacaaac tgcatttgaa aataaagcca ctgagttttc
     4201 cgatgtacta aaaatgggaa gaacgcagtt acaagatgcc gtgccaatga cgctcggtca
     4261 ggaatttaaa gcttttgccg tattattaga tgaagaggtt cgtaacctta aacggacggc
     4321 agaattgctt cttgaagtga atttaggcgc aaccgctatc ggtactggtc tgaatacgcc
     4381 agagggttac tctactcttg cagttaaata tttagccgaa gtcacgcaac tgccttgctc
     4441 actgtctgaa aatctcattg aggcaacatc tgactgtggt gcttatgtaa tggttcacgg
     4501 tgcattaaaa cgcaccgccg tcaaactttc taaagtgtgt aatgacttac gattactttc
     4561 atcaggccca cgtgccggct taaatgaaat taatcttccg gaattacaag ccggctcatc
     4621 tattatgccg gcaaaagtaa accctgttgt acctgaagtc gtaaaccaag tgtgctttaa
     4681 agtgattggt aacgacacca ccgttacttt tgcagcagag gccggtcaat tacagcttaa
     4741 cgtaatggag ccggttatcg ggcaagcaat gtttgaatct atcgagctgc tttccaatgc
     4801 atgtattaat ctacgtgata aatgtattga cggtattacc gctaaccgtg acatctgcca
     4861 aaactatgtg ttcagctcta ttggtattgt gacctacctc aatccattta tcggccacca
     4921 taatggtgat attgtcggca aaatttgtgc aaccaccggc aaaagtgtac gagaagttgt
     4981 gcttgagcga ggcttactca ccgaagaaca actcgatgat attctctcga ttgagaactt
     5041 aatgaatccg acttataaag ccaaacgctt taccgaagag gaataatcat tgtttacaag
     5101 cggtctgatt ctggcaaaaa attgcaaatt tttaccaaaa tcagaccgct tgttatccca
     5161 ttatttctta tttgctttca gcatcgccat taaatttgca atatttgcag aagattttgc
     5221 ctctttttcc tgttcggtca ttcttggctt ttcactttcc cactccaaat catcttgtgg
     5281 caattccagt aaaaaccggc tcggctcagg tttgatgatt tcgccatatt ggcgacgttc
     5341 tttacacagt gaaaacgtga gcgtttgttg ggctcgggta atgccgacat aagccaaacg
     5401 ccgctcttct tctacattgt cctcatcaag gctggtttgg tgtggcagaa tcccctcttc
     5461 cataccgatt aagaaaacgt gcggaaattc caaccctttc gaggcgtgaa gagtcattag
     5521 ttgaacttga tcggcttcat cgtcctcttc gccacgttcg agcatatctc gcaaggttaa
     5581 acggtttacc acttgcacca aattcatcgg aggctctatt tcatcgccct ctaacatcgc
     5641 ttgcacccat tcaaacaagg tctgcacatt acggctttgc atttccgcta cttttgggtt
     5701 cgccgccatt tcatacaaat aggcttcata ctgaattttc gccaacagat cttttactgc
     5761 ttcaatcgga tcggatctat cggcttgatc ggcaatttcc acaatccagc gggcaaaatc
     5821 ctgtaaggct tggtaaggct ttggcgtgag gcgttgtatg agttcaaaat caaaaatcgc
     5881 ctcaaacaaa ctgacctgct tttcattagc aagtatgcct aatttttcta gcgttgccgc
     5941 cccaatttcc cgtttcggcg tattcacaat acgcaagaaa gctgcatcat cgtcttgatt
     6001 cactaccaac cgcagatacg ccatcatatc tttgatttcc gctcgagcaa aaaatgacgt
     6061 accacccgaa attttatacg gaatgcggtt ttgcattagc aatttttcca acaagcggga
     6121 ttgatggttg cctcgataca aaattgcata atctttgtat ttggtctttt tcgagaaacg
     6181 atgggcaatc agctcgccca caatgcgttc cgcttcgtgt tcctcatttt ttgcctcaat
     6241 gacctgtaat ttttcccctt gccccagttc tgaaaacagc cgcttgctaa acaagtgatc
     6301 gttattatca atcagaatat tggcacaatg cagaatccgc tggctggaac ggtaattttg
     6361 ctcaagttta atcacttgca aatcaaaatc ttcccgtaac cgttgcatat tttcaggctt
     6421 cgccccacgc caagagtaaa ttgattggtc atcatcgccc acgaccgtaa agtttggctc
     6481 gttgccgaca atcagcttaa ttaactcata ttggctggtg ttggtgtctt gatattcatc
     6541 aactaacaaa tagcgaattt tctgttgcca acgagatttt gcttcgggaa attggcggaa
     6601 cagcaacacc ggcaacataa tcaaatcatc aaaatccaac gcattataag ccttaagttg
     6661 attctggtag agctgataaa aatgtgaaaa cactcgctca cgttcatcac gcacacgagt
     6721 aagtaccctt tcaggcgaaa gcaaatcgtt tttccagtta gaaatagtgg caattaaggc
     6781 tttcagcaga tccttatctt ccgtcacatt ttcaggcaat aaatgtttca gcaaagcaat
     6841 ttgatcctgc tcatcaaaca aggtcatacc cgatttaaaa ccgagcaatt tatattcgcg
     6901 ttttaaaatt tcaaacccaa gtgtgtgaaa agtcgaaatg gttaaacctt tgctgttttc
     6961 tttgccgata gaatgatcca cccgttcacg catttcccga gcagctttgt tggtaaaggt
     7021 tactgctgca atctgcttag gcgaatagcc acaatgtgca attaaatgtg cgattttatt
     7081 aataatcacc cgagtcttac ccgagccggc accagccagc acaaggcaag cccctttggt
     7141 atattctact gcttgttgtt gttgagaatt gagtttcatt gaatctattc aaaagtaatt
     7201 ttgacaacta ttttaacgga aagaaaacaa gcggtcgaat tttcaggaaa atttgcaaaa
     7261 aactttgaaa atccgaccgc ttgttcatca gagaaaaatc cccaaaacgc aaatttttcc
     7321 tttcaatctt gcccctttag ctatatcatt ataagttgat tttttagggg caaatgcctg
     7381 attttataac tccttttctt tttaatttaa gggaaaaaaa cagtagatgg aaaaagtaga
     7441 acagaaagct atcattgagc ttaaaaatgt aacaaaaagc tacggtagta aaacgattct
     7501 taaaaattta aatttaacca ttaacgatgg tgaattttta acaattttag gcccttccgg
     7561 ttgtggtaaa acaactgcgt tacgtttaat tgccggtttt gaagatttaa ctgaaggttc
     7621 aattattctt gatgggcaag atgtttctaa cgttccggcg gagcaacgcc cggtaaacac
     7681 cgtattccaa agctatgccc ttttcccaca tatgacgatt tttgaaaacg tggctttcgg
     7741 gctgcgtatg caaaaagtgc caaatgctga aattgagcca cgtgtgatgg aagcattacg
     7801 aatggtgcgt ttagaagacc gcgctcaaca aaaaccggct cagctttccg gtggtcagca
     7861 acaacgtatt gcaattgccc gtgcggtggt aaataaaccg aaagtattgt tgttagacga
     7921 atcgctttct gcgttagatt ataaattgcg taaagagatg caaaatgaat taaaagcatt
     7981 gcaacgtcaa ttaggcatta cctttatttt tgtgacccac gatcaggaag aagccttaac
     8041 catgtctgat cgtattatcg taatgaacgc tggtaaaatc gctcaagacg gtacaccacg
     8101 tgagatttac gaagagccaa gtaacttatt tgtagctcgt ttcatcggcg aaattaacgt
     8161 ttttgatgca actgtaattg agcgtgttga tgcaactaat gtaaaagcaa acgtagaagg
     8221 tcgcatttgc aatatcaaag tggaagattt agaggttcag ccgaaccaaa aattaaaagt
     8281 attgttacgc cctgaagata ttgtgattga ggagttagac gaaaacgaaa gctcgaaagc
     8341 aattatcggt catattatcg accgtaacta caaaggtatg acattagagt ctagcgtgag
     8401 attagatcat aacggtatga atgtattggt tagcgagttc ttcaatgaag acgatccgaa
     8461 cattgaccac gaagtgggac aaaaagttgc tcttacttgg tatgaaggct gggaggttgt
     8521 gttaaacgat gaaggcgaat aacaaattcc aaaatggtgc ggtcgcaact attttcggtt
     8581 ggttattact tttcgtatta gtgccgaacg tactcgtttt aatcatcagc ttcttaacag
     8641 aaaaccgtca aagtcagtat tttgtggact ttgcatttag ttttgatgct tataaatcac
     8701 tgtttaatga cacctatgca gcagtattgt ggaactcgct atatatggcg ggcattgcca
     8761 cctttttctg tttgatcatc ggctatccct ttgcattttt tattgccaaa atgccggaaa
     8821 gaattcgtcc gtttttactg tttttagtag ttctaccgtt ttggactaac tcactgattc
     8881 gtatttatgg cattaagatt ttcttaggcg taaaaggctt tttaaatgaa agcttgatgg
     8941 ctttaggtat aatcagcgag ccaatgcgtt tattaaactc agaaattgcg attattattg
     9001 gtttagttta cattctatta ccttttatga ttctaccgct ttattcatca atcgagaaaa
     9061 ttgataaccg cttactagaa gctgcaaaag acttaggtgc aaacgccttc cagcgtttta
     9121 ttaaagtgac tattccatta acaatgccgg gcattgtcgc aggttgttta ttagtattac
     9181 ttccggcaat gggtatgttc tatgtggcag atttattagg cggcggtaaa acaccgttag
     9241 taggtaacat tattaagagc ttgttcttaa ataccaacca atttgcatta ggttcagcag
     9301 taagtattgc attaactatc ttaatggcat taatgcttta tgtttactat cgtgcaaaca
     9361 aattattaaa taaaaaggtg gagcttgaat aatgaaaaag atgtttagaa acctttttat
     9421 gttggttgtt tttgcatatc tctatattcc aattatcatt ttagtgatca actcctttaa
     9481 tgaagaccgc tttggattaa attggaaagg ctttacttgg aattggtatg agcgtttatt
     9541 cagtaatgat acgcttgtac aagcaacatt gaactcatta actattgcgt tctttgcggc
     9601 aacattctca accattttag gggcattaac ctctattgcg ctttaccgtt accgtttccg
     9661 tggtaaaaag ctagtgggcg gaatgttatt cgtggtaatg atgtcgcctg atatcgtaat
     9721 ggcggtatct atgctcgtat tatttatgat cttaggtatc aagctaggct ttatttcgct
     9781 attaattgca cacattactt tctgtttgcc ttatgtggta attgcaattt cctcacgcct
     9841 cagcgatttt gacggcaaaa tgctagaagc tgcaaaagac ctaggtgcaa gtgaagtgac
     9901 tattttgcgt aaaattattt taccactggc attaccttcg attatttcag gctggttgtt
     9961 aagctttacg atttcgttag atgacgttat tgtttcatct ttcgtgacaa gcccaagcta
    10021 cgaagtttta ccactgaaaa ttttctcatt ggttaaaaca ggtgtaacac ctgaagtgaa
    10081 tgcactagca actattatga ttattttctc attaacatta gtggtattaa gccagttagt
    10141 agtgaagaaa aaagtctaaa aatcactaaa
//
LOCUS       DANLXS010000083        10012 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.083, whole genome shotgun sequence.
ACCESSION   DANLXS010000083 DANLXS010000000
VERSION     DANLXS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10012
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.083"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..310
                     /locus_tag="PXD71_001518"
     CDS             <1..310
                     /locus_tag="PXD71_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291628.1"
                     /translation="FNLENVLIDFPNKTNSVAYQNLVIHNAKTRAGKLKTQYANAHDL
                     GNLVLVEDIQKIFSGLLEIIYQVRCHLVHGSLSPTPENHEVVKYCYLILWDCLKGFCD
                     "
     gene            complement(578..1315)
                     /locus_tag="PXD71_001519"
     CDS             complement(578..1315)
                     /locus_tag="PXD71_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6291629.1"
                     /translation="MIKIENLTLAHGIQHLSCYLPKGKLIGIMGANGAGKSSLLKAIA
                     GILRPTSGEIWLENCKLHALSAVERSRQFAYLAQQLNVAWQLSVYDVIALGLPAPLPP
                     EQERQKVRSVAEQLAISHLLDNTYQQLSGGEQTRVQLARCLMKQAPILLADEPIAALD
                     PYYQIDIMQQLKALTPTQTCVVVIHHLPLAYRFCDEIILLKNGNLLVSGNTQAVLNAE
                     NIATAFGVKAEMDWEKREMWGIEKLEV"
     gene            complement(1312..2280)
                     /locus_tag="PXD71_001520"
     CDS             complement(1312..2280)
                     /locus_tag="PXD71_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6291630.1"
                     /translation="MKKLNIVLGSLLLLIVSNAAFYQLSDFALLKNAEGVLSDLRWLV
                     LGEIRLPRIGLAVLTGASLALAGNAMQGVFKNPLASPGLLGSSSGATAVSVFLLYYFS
                     APMGWLLFGGVAGALGAFLLVWLISFRHGTTMMILSGVAVNVLLGAAVTLLLSNAESP
                     WALAELYRWLQGSLVWATAEAVCWALPLILLGIVCLYRERRYLDLLTFGEETAATMGI
                     NLQRSFFTVSIGVALLVGATIPQTGTIGFIGLIAPHLARIWLKKPPSQLYLTSALIGA
                     LLLLIADLAVQYVPFFARIYIGTLTAILGVPFLIWILFTQQWRLAQ"
     gene            2461..2796
                     /locus_tag="PXD71_001521"
     CDS             2461..2796
                     /locus_tag="PXD71_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6291631.1"
                     /translation="MYHIERTEIFDQWLENLKDPLAVVTIARRIERAESGNFGDTKYI
                     AEGISEMRLNVSKGYRLYYAQKGSIIYILLNGGHKGTQQADIDKAKMLWAEIKQHMAE
                     QEKKNERII"
     gene            2780..3103
                     /locus_tag="PXD71_001522"
     CDS             2780..3103
                     /locus_tag="PXD71_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL6291632.1"
                     /translation="MNELYDAPIPQATREKFGIQPFDAAEYLANDELIALYLAETLKD
                     GTDEEFIQALNTVARAKGMNELAKKAGIGRESLYQSLSSSKPRFETIRKIISALNLEL
                     SVVKA"
     gene            complement(3093..4979)
                     /locus_tag="PXD71_001523"
     CDS             complement(3093..4979)
                     /locus_tag="PXD71_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL6291633.1"
                     /translation="MTQLDSVTVYSAYATPINQDKTASSVTVLTEKDFAARNATYVSD
                     VLKTVPGVAIGQQGGRGTLTSLFLRGAESRHTAVVIDGVKVNPINIGNFDFGGLPISN
                     IERIEVLRGEQSALWGSSAMGGVVYITTKSGLYKEKPFNAEVDLGLGSNNTRDASATL
                     SGFHNGFYYALHGDSHRTKGISALSKNHFSYTTETGSEVKTGGASERDGFHRDNGSLR
                     LGYDLGNKGVEVLAAQSSQTVHIDGYNSDVSGEYSRTRNQTFKLGGYWGNEQELLKHQ
                     ANISQFNSKATHFGSNARYSNEKQLNANYQLDVNFDREGEVTQAVSLLTDYAKTRYTS
                     DKYLREKTLSEKSAALEYRLFTEQDHSFSISGRYTDNSQFKNSITGRISGAYRLSPNL
                     CSDRLLLELAEPQQIRAMSPYSQKPLMMLDKLNTDKPTVEPELTALLPYADSTILLNE
                     TFYPQLTARLKQLGFKLVALNDSPQTPEQLFTLILQLGELTQNQAKAEKLVERLRLQK
                     IPLKQPLAETLILSETGMIEPHFPQYQTLLDLLGLSPLKSDLTPQNFSLEKLLLAQPK
                     QLLFLTDNQSYNNQAELLKHPALQKIWQKMSQNPPLVLPMKYTYCFDHGVWQGIQLMH
                     KLTP"
     gene            5009..5809
                     /gene="metF"
                     /locus_tag="PXD71_001524"
     CDS             5009..5809
                     /gene="metF"
                     /locus_tag="PXD71_001524"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDL6291634.1"
                     /translation="MQQKWRMLWDSIDRLSPLKPKFVSVTYGANSGERDRTHSIVKSI
                     QEKTGIISAPHLTGIDATPEELKVIAKDYWDSGIRRIVALRGDIPKGYDKALFYASDL
                     VALLKSVADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGANHVITQFFFDVESYLR
                     FRDRCVSIGIDCEIVPGILPVTNFKQLQKMAAVTNVKIPTWMGKMYEGLDDDQTTRNL
                     VAASIAMDMVKVLSREGVNDFHFYTLNRSELTYAMCHTLGVRPDLTTA"
     gene            complement(5858..7018)
                     /locus_tag="PXD71_001525"
     CDS             complement(5858..7018)
                     /locus_tag="PXD71_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291635.1"
                     /translation="MKKSILLILSLFSASLFADVQLSPLPLPQIGKVSQFDLSEQGEL
                     VVINQQKQLWRVTQQAQKLADGVSTEIEPKAQYQRIALADQNGHFMLWTADKIYTSNI
                     PLAPKATMTALNFATIAVTKQNGQFKLARIETQGNQATITATAETEVLPDAHPLQIDF
                     DAETPNQGHIAVLAKPDDFTYQHGVLGDDVEAGEIQFLERHTLNPLAEKLTQQGRVFE
                     ANRWATFTENGKTKIVSVISGDGNGASTVVIGVKDGKLSIEAESEPLSTNRWQSPFIF
                     NRKLYAIQMPHLKGNLVEYSLKGNQLAERFITSGLSNHRYGDHETNLVATTETFALIP
                     QMGYQQVVVLDKTGKITELPTALPAGIKQTKADSNKAYLLLKNGEIWVAEEK"
     gene            complement(7078..8094)
                     /gene="galE"
                     /locus_tag="PXD71_001526"
     CDS             complement(7078..8094)
                     /gene="galE"
                     /locus_tag="PXD71_001526"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL6291636.1"
                     /translation="MAILVTGGAGYIGSHTLVELLNENREIVVLDNLSNSSEVSLERV
                     KQITGKSVKFYQGDILDRDILRKIFAENQIESVIHFAGLKAVGESVREPLRYYQNNVT
                     GSIVLVEEMLKANVNTIVFSSSATVYGDPQIIPIVESCPVGGTTNPYGTSKYMVERIL
                     EDTVKAFPQLSAVVLRYFNPVGAHESGLIGEDPNGIPNNLMPFISQVAVGKLPQLSVF
                     GGDYNTHDGTGVRDYIHVVDLALGHLKALDKHQNDAGFHVYNLGTGTGYSVLDMVKAF
                     EAANGITIPYKVVDRRPGDIAVCYSAPQKALEQLGWETERGLEQMMKDTWNWQKNNPN
                     GYKG"
     gene            complement(8182..8343)
                     /locus_tag="PXD71_001527"
     CDS             complement(8182..8343)
                     /locus_tag="PXD71_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HDL6291637.1"
                     /translation="MNETLLNNLVCPVSNEKLEWDKENNRLINPKLNIAYPIKNGIPE
                     LLPEAGVAL"
     gene            complement(8321..9322)
                     /gene="lpxK"
                     /locus_tag="PXD71_001528"
     CDS             complement(8321..9322)
                     /gene="lpxK"
                     /locus_tag="PXD71_001528"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDL6291638.1"
                     /translation="MKIWQTKSLITWLLSPLSLLFWLISQARKRLYSLNILQSYRSPL
                     PMLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVISRGYGGKAKTFPQLVTRESSAE
                     MVGDEPVLIVQRTNVPLAISPNRQQSIELLLNQFEPDIIITDDGLQHYALQRDMEWVV
                     VDGVRRFGNGFVLPAGGLRELPSRLNSVQAVICNGGTPQANEHLMTLEPSDAINLVTN
                     QRKPLSEFTQPCVAIAGIGHPPRFFTMLEKLGIPLAKTQGFADHQAFDKAQLAQFADS
                     QTPLLMTEKDAVKCHDFAQPNWWYVPVSATFSEKSTACLLAPILQKLGKEDERNTTQ"
     gene            complement(9324..9728)
                     /locus_tag="PXD71_001529"
     CDS             complement(9324..9728)
                     /locus_tag="PXD71_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6291639.1"
                     /translation="MKVHEKIKLLRENNHWTQDEMAQKLSMTTKGYAKIERGETISNL
                     PKIEQIAEVFNMDICELLSYGEEGKIYINNTDNNLTNSFISLGNGVEEIQRLQLIISH
                     KDELLAHKEEIIENQKRELALLRDLVETLKNK"
BASE COUNT         2752 a         2137 c         2195 g         2928 t
ORIGIN      
        1 atttaacttg gaaaatgtgc ttattgattt tcctaacaag acaaattctg tagcatatca
       61 gaatctagtt attcacaacg caaaaacgcg tgcaggaaaa ttaaaaacac aatatgcaaa
      121 tgctcacgat ttaggcaatt tggttttagt agaggatatc caaaaaatat tttccgggtt
      181 attagaaata atttatcaag ttcgttgcca tttggtacat ggtagcttaa gtccaacacc
      241 tgaaaaccac gaagttgtta aatattgcta tttgattttg tgggattgtt taaaaggatt
      301 ttgtgattag ttattgtcaa taaagctaca agcggtcaat tctcccaaaa ctttgcaaat
      361 ttccccgcaa aactgaccgc ttgttctcct ctatccattc acctcaacca acttttctct
      421 ctacccctac tcaaaatcgg ttaaacaatc tgttgaggta aatgttgtgt cggaatggga
      481 tttaatctta atgaaactga aaaaatcttc tgccaagcaa atttccctaa aattggaagt
      541 tcatttccaa tttctcaaaa aatcacccaa accggaacta cacttccaat ttttcaatcc
      601 cccacatttc ccttttctcc caatccattt cagctttcac cccaaaagcc gttgcaatat
      661 tttcggcatt taagaccgct tgtgtgttgc cgctgacgag taaattgccg tttttaagca
      721 gaatgatttc atcacaaaaa cggtaggcaa ggggcaaatg atgaatcacc accacgcagg
      781 tttgtgttgg agttagggct ttgagctgct gcataatatc gatttggtag taggggtcga
      841 gggcggcaat cggttcatcg gcaagcaaga tcggggcttg tttcatcaaa caacgggcga
      901 gttgcactcg ggtctgttca ccgccggaga gctgttggta agtgttgtcc agcaggtgag
      961 aaatcgccaa ttgttcggca accgaacgca ccttttgacg ctcttgttca ggcggaagcg
     1021 gtgcgggcaa gccgagtgca atcacatcat acaccgaaag ttgccacgcc acgttgagct
     1081 gttgggcgag gtaggcaaat tgtcggctcc tctcaacggc ggaaagggcg tgcagtttgc
     1141 aattttccag ccaaatttca ccgcttgtcg ggcgtagaat gccggcaatc gctttgagca
     1201 acgaggattt ccctgccccg tttgcgccca taatgccgat caatttgcct tttggcagat
     1261 agcaagagag gtgttgaata ccgtgggcaa gggtgagatt ttcaatcttg atcattgtgc
     1321 caatctccat tgctgggtga ataaaatcca aatcaaaaac ggtacgccca aaattgccgt
     1381 gagcgtgccg atgtaaattc gggcaaaaaa cggcacatat tgcaccgcca gatctgcaat
     1441 cagcagtaac aacgccccga ttaaagcgga ggtaagataa agctgggacg gcggtttttt
     1501 cagccaaata cgggcaagat gaggggctat caagccgata aaaccgatgg tgccggtctg
     1561 tggaatcgtt gctccgacca acagcgccac gccaatgcta acggtaaaaa aactgcgttg
     1621 caggttgatg cccattgttg ctgccgtctc ttcgccaaag gtgagcaggt cgagataacg
     1681 ccgttcccga tagaggcaga caatgccgag caagatcaac ggtaacgccc aacatactgc
     1741 ctctgcggtt gcccagacta atgagccttg caaccagcgg tagagttccg ccaacgccca
     1801 cgggctttcg gcattggaaa gcagtaaagt caccgctgcc ccgagcaaca cgtttaccgc
     1861 cacgccactt aaaatcatca ttgttgtgcc gtggcgaaag ctaatcagcc acaccagcaa
     1921 aaatgccccc agcgcccctg ccacaccgcc aaacagtagc cagcccatcg gggctgaaaa
     1981 atagtagagc agaaatacgc tcaccgccgt tgcccccgaa ctgctcccaa gcaaaccggg
     2041 gctggcaagc ggatttttaa acacgccctg catcgcattg ccggcaagcg caaggctggc
     2101 gcccgtcagc accgccaagc caatacgggg cagtcgaatt tcacccagca ccagccagcg
     2161 cagatcgctt agcacgcctt ccgcattttt aagcaaggca aaatcgctca gttggtaaaa
     2221 ggctgcgttg ctaacaatca gcaacaacaa gcttccaagc acaatgttta atttcttcat
     2281 tcttctttct ctcatcaaac gtgcaaatta taccatagcg ccaaagtcaa gctgaaaacg
     2341 ctatttttgc gatcatcatc gaaaaactag cgtttgccga atgtaaccca cgacaggctt
     2401 tgctatattc cttcaatcag cctaagtttt gtatttaaaa cattacagat tgggggtaga
     2461 atgtaccata ttgaacgcac tgaaattttc gaccaatggc ttgaaaattt aaaagatcca
     2521 cttgctgtgg tgaccattgc aagacgtatt gaacgtgccg aaagcggaaa ttttggcgat
     2581 accaaatata tcgcagaagg aatttccgaa atgaggctaa acgtcagcaa aggttatcgc
     2641 ctttactatg cgcaaaaagg tagcattatt tatatactac taaatggcgg acataaaggc
     2701 acgcaacaag ctgatattga taaagctaaa atgctatggg cagaaattaa acaacatatg
     2761 gcagaacagg agaaaaagaa tgaacgaatt atatgacgcc ccaattccac aagctactcg
     2821 tgaaaaattt ggcattcaac ctttcgatgc cgctgaatac ctcgccaatg acgagctaat
     2881 cgccctttat ttggcggaaa cattgaaaga cggcacggac gaggagttta ttcaagcatt
     2941 gaataccgtg gcacgagcga aaggaatgaa tgagttagcg aaaaaagcag ggatcgggcg
     3001 tgaaagtttg tatcaaagcc tttccagcag taagccccgt tttgagacta tccgcaaaat
     3061 tattagcgca ttaaatttgg agttgtcggt cgttaaggcg taagtttgtg cattaattgt
     3121 atcccctgcc aaaccccgtg gtcgaaacag taggtgtatt tcatcggtag gacaagcggt
     3181 ggattttggc tcattttttg ccatattttt tgtaatgctg ggtgtttgag cagttcggct
     3241 tggttgttat agctttggtt atcggtgagg aacaataact gtttgggttg cgctaacagc
     3301 agtttttcta gcgagaaatt ctgtggcgtg aggtcgcttt ttaatggtga cagaccgaga
     3361 agatccaaca acgtttgata ttgcggaaaa tgcggttcga tcatacccgt ttcgcttaga
     3421 attaaagttt cagcaagcgg ttgttttagc ggaatttttt gcaaacgtag ccgttccacc
     3481 agcttttccg ctttggcttg attttgggtt agctcgccca gttgtaaaat cagtgtgaaa
     3541 agctgttcgg gtgtttgcgg actgtcgttt aatgcgacta acttaaagcc aagctgtttc
     3601 agccgtgcgg tgagttgcgg atagaaggtt tcattcagca aaatggtgct gtcggcatag
     3661 ggcaacaggg cggtgagttc aggctcaacg gtgggcttgt ccgtgttcag tttatccaac
     3721 atcatcaacg gcttttggga atagggcgac atcgcccgaa tttgctgtgg ttcggcaagt
     3781 tccagcaaca gacggtcgct acacaaattt ggcgataaac ggtaagctcc tgaaatacgc
     3841 ccagtgatag aatttttaaa ctgtgaatta tcggtgtaac ggccactaat agaaaaactg
     3901 tgatcttgtt cggtaaataa gcgatattct agtgccgcac ttttttcgga taaggttttt
     3961 tcacgcaaat atttatcaga ggtatagcgt gtttttgcat aatctgtcag taaactgacc
     4021 gcttgtgtca cttctccttc acggtcaaag tttacatcta attgataatt cgcattaagt
     4081 tgtttttcgt tgctgtagcg agcatttgaa ccgaaatgtg ttgctttact attgaattgg
     4141 ctgatatttg cttggtgttt cagcagctct tgttcatttc cccaataacc gcctaattta
     4201 aaagtttgat tgcgagtacg ggaatattca ccgctaacgt ctgaattata gccatcaata
     4261 tgaacggttt gggaagattg agcggcaaga acttctaccc ctttgttacc gaggtcataa
     4321 cctaaacgta aagagccgtt atcacgatga aagccatctc gttcgcttgc accaccggtt
     4381 ttcacttctg agcctgtttc tgtggtatag ctgaaatggt ttttactcag tgcggaaatg
     4441 cctttggtac gatggctatc accgtgtaag gcatagtaga aaccgttgtg aaatccggaa
     4501 agtgttgcgc tggcatcacg ggtattattt gagcctaaac caagatcgac ctccgcattg
     4561 aatggtttct ctttgtataa accgctttta gtggtgatat aaactacacc acccatagca
     4621 ctgcttcccc aaagggcgga ttgctcgcca cgcaacactt cgatacgttc gatattgcta
     4681 atcggtaagc caccaaaatc gaaatttcca atattaatcg gattgacttt tacaccgtca
     4741 atcacaaccg cagtatggcg agattctgcc cctcgtaaaa ataagctagt aagagtacca
     4801 cgcccacctt gttgcccaat cgccacgccc ggcacagtct ttaacacatc actcacataa
     4861 gtcgcattgc gtgcggcaaa atctttttcg gtaagaaccg tcacgctgga agcggttttg
     4921 tcttggttga ttggggttgc ataggcggaa tagactgtta cggaatcaag ctgagtcatc
     4981 tcattttgtg caaaagctgt acttgctagt gcaacagaaa tggcgaatgc tgtgggattc
     5041 tattgatcgc ctaagtcctt taaaaccgaa atttgtttcg gtaacttatg gagcaaattc
     5101 tggtgagcga gatcgtacac acagtattgt gaaatcaatc caagaaaaaa cagggatcat
     5161 ttcagctccg cacttaacag gcattgatgc aacaccggaa gaattaaagg taattgccaa
     5221 agattattgg gatagtggta ttcgccgaat tgttgctttg cgtggcgata taccgaaagg
     5281 ttatgataaa gcactatttt acgcctcaga tttagtcgct ttattaaaat cagtagcaga
     5341 tttcgatatt tccgtggctg cctaccctga agttcaccct gaagcaaaat cagcacaggc
     5401 agatttactt aacttgaaac gtaaaatcga tgcgggtgca aaccacgtta ttacgcaatt
     5461 cttttttgat gtagagagtt atttacgttt tcgtgatcgc tgtgtttcta ttgggattga
     5521 ttgtgaaatt gtgccgggta ttctgccggt gacaaatttc aagcagttgc aaaaaatggc
     5581 agcagtgact aatgttaaga tcccgacttg gatgggtaaa atgtacgaag ggttagatga
     5641 tgatcaaacc accagaaact tagttgcagc aagtattgca atggatatgg tgaaagtgct
     5701 ttctcgtgaa ggggtgaatg atttccactt ctatacctta aatcgtagtg aactcactta
     5761 tgcaatgtgc catactttag gcgtaagacc tgatttaaca actgcataat cgaacattaa
     5821 ggctgtatag ctaaatgagc tatacctcct tatagtttta cttctcttca gccacccaaa
     5881 tctcgccatt ttttaataat aaataggctt tgttggaatc ggctttggtt tgtttaattc
     5941 cagccggcag ggcggttggc aattcagtga tcttgcctgt tttatccaac acaacaactt
     6001 gttgatagcc catttggggt atcagtgcga aagtctctgt tgtcgccact aaattggttt
     6061 cgtgatcacc atagcggtga ttgcttaagc cggaggtaat aaaacgttca gctaattggt
     6121 ttcctttcag gctgtattca accaaattgc cttttagatg tggcatttgg attgcataga
     6181 gcttgcggtt aaagataaac ggcgattgcc aacggttagt cgacagcggc tcactttcgg
     6241 cttctattga taattttccg tctttaacgc caatcacaac agtgctggcc ccattgccat
     6301 cgccagagat aacagaaacg atcttggttt ttccattttc tgtgaaagtc gcccagcggt
     6361 tggcttcaaa tactcgccct tgttgggtga gtttttctgc taaggggttt agggtatggc
     6421 gttctagaaa ttgaatttcg cctgcctcta catcatcgcc taatacaccg tgttgatatg
     6481 taaaatcatc aggctttgca agtacagcaa tatgcccttg gttaggtgtt tcggcatcaa
     6541 aatcaatttg cagagggtga gcatcaggca acacctcggt ttctgcggtg gcagtaatag
     6601 tggcttggtt tccttgcgtt tcaattctgg ctaacttaaa ttgtccgttt tgtttggtca
     6661 cggcaatggt ggcaaaatta agggcggtca ttgtagcttt cggggcaagg ggaatgttgg
     6721 aggtatagat tttatcagcc gtccacagca taaaatgccc gttttgatcc gctaacgcaa
     6781 ttcgttggta ttgagcttta ggttcaatct cggtggaaac tccatctgcc agtttttgag
     6841 cttgttgtgt gactctccat aattgttttt gttggttaat taccactaat tcgccttgtt
     6901 ctgaaaggtc aaattggctt actttgccaa tttggggaag tgggagtgga gaaagctgaa
     6961 catcagcaaa caaagaagcg gagaacaagc ttaagattaa cagaatagat tttttcatta
     7021 gcgttaccta aaaagaagat ttaaatgata aagggagatg ttatctccct ttacgcttta
     7081 gcctttataa ccattcgggt tatttttttg ccaattccaa gtgtctttca tcatttgttc
     7141 tagcccacgc tcggtttccc aacccagttg ctctaacgct ttttgtggag cggagtagca
     7201 aacggcaata tcgcccgggc ggcgatctac cactttgtat ggaatagtaa tgccatttgc
     7261 ggcttcaaag gcttttacca tatctagcac cgaataacct gttcctgtgc ctaaattgta
     7321 aacgtggaaa ccggcatcat tttggtgctt gtctaaggct tttaagtgac caagtgctaa
     7381 atctaccacg tggatataat cacgcacacc tgtgccatcg tgagtattat aatcgccacc
     7441 aaacaccgat aattgaggta atttgcccac agccacttgg ctgataaacg gcattaagtt
     7501 atttgggata ccgtttggat cttcaccgat taaaccgctt tcgtgagccc ctaccgggtt
     7561 gaagtaacgc aatactaccg cacttaattg tgggaaggct ttaacggtat cttcaagaat
     7621 gcgttcaacc atatatttgg acgtaccgta agggttggtt gtgccaccga cagggcaaga
     7681 ttccacaatc gggataatct gcggatcacc ataaacggtc gccgatgaac taaacacaat
     7741 ggtattcaca ttggctttta gcatctcttc taccaataca atcgagccgg ttacattgtt
     7801 ttgatagtaa cgtagcggtt ctctgacgct ttcgcctaca gcttttaaac cggcaaagtg
     7861 aattaccgat tcaatctgat tttccgcaaa aattttgcgt aaaatatcac gatctaaaat
     7921 atcgccttga tagaatttta cgctcttgcc ggtgatctgc ttcacacgct caagtgatac
     7981 ttcagaggaa ttagaaagat tatctaacac cacgatttca cgattttcgt ttaataattc
     8041 aactaatgta tgtgaaccga tgtagcctgc accacctgta actaaaattg ccattttatg
     8101 ttcctctata aaattaatac gaaccaaggt tattgttgct attttacccg atttaatcag
     8161 gcttgctaat atttattcag tttataatgc cactccggct tccggcagta attccggaat
     8221 gccgttttta atcgggtaag caatattgag cttagggtta attaatcggt tgttctcttt
     8281 gtcccattct aatttttcat tagagacagg gcagacgagg ttattgagta gtgtttcgtt
     8341 catcttcttt tcctagtttt tgtagaatcg gggctaacaa acaagcggtc gatttttctg
     8401 aaaatgttgc agaaaccggt acataccacc aatttggctg tgcaaaatcg tggcatttta
     8461 ccgcatcttt ctcggtcatt aataacggcg tttggctatc agcaaattga gccagctgag
     8521 ccttatcaaa ggcttgatga tcggcaaagc cttgagtttt ggcaagcgga atgcctaatt
     8581 tttctaacat cgtaaaaaag cgtggtgggt gaccgatacc ggcaatcgcc acacagggtt
     8641 gggtaaattc gcttaatggc ttgcgttgat tggtgactaa attaattgca tcagacggtt
     8701 caagcgtcat taaatgctcg ttagcttgcg gtgtgccgcc gttgcaaata accgcttgca
     8761 cggagtttaa acggctcggt aattcacgca atccgcccgc cggcagcaca aaaccgttac
     8821 cgaaacgcct tacgccatca accaccaccc attccatatc tcgctgtagg gcatagtgtt
     8881 gcaagccatc atcggtgatg atgatgtccg gctcaaactg atttaatagt aattcaatgc
     8941 tttgttggcg gtttggagaa atagccaaag gcacattggt tcgctgaaca atcagaaccg
     9001 gttcatcacc caccatttca gcagaacttt ctcgggtaac taattgcgga aacgttttcg
     9061 ctttaccgcc atagcctcga gaaatcacgc ctacttttac gccacgctgt tgcagttgtt
     9121 ccaccagcca aacgaccact ggcgttttgc cgttgcctcc aaccgagata ttaccgacta
     9181 ccagcatagg caaaggcgaa cggtacgatt gcaaaatatt cagagaatat aaccgcttgc
     9241 gagcttgact aatcagccaa aacagcaacg aaagcggcga aagcagccaa gtgataaggg
     9301 attttgtttg ccagattttc atattattta tttttaagtg tttcaactaa atctcgtaat
     9361 aaggctaatt cacgtttttg gttttcgata atttcttctt tatgggctaa taattcatct
     9421 ttgtgggaga taataagttg taaacgttga atttcttcta ctccatttcc taatgagata
     9481 aatgaattag tgagattatt atctgtatta tttatgtaaa tttttccttc ttccccatag
     9541 gataataatt cgcaaatatc catattaaat acttctgcga tttgttctat tttcggtaaa
     9601 ttagagatag tttcaccacg ttctattttt gcataacctt tggttgtcat acttaatttt
     9661 tgagccattt catcttgtgt ccaatgatta ttttctcgca gtaatttgat tttttcgtgt
     9721 actttcatat aacacctata gattgtggga gttgggatat aacttgatgt ggtaaaggat
     9781 aaattttgtg gattgtagca gtttattgag caatttagaa tagagtaaat tttatcttaa
     9841 gctcacagga gaagaaacat tcatgaataa tcaatataat ccattatatg gaatgctagg
     9901 tattttagcg tcagctgcat cagtaatttt gcctctttat gcagcatata aagactatca
     9961 atttggggag aatatgttat ctcttatgga tataattatt ttcccgttag gt
//
LOCUS       DANLXS010000084         9892 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.084, whole genome shotgun sequence.
ACCESSION   DANLXS010000084 DANLXS010000000
VERSION     DANLXS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.084"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(47..541)
                     /locus_tag="PXD71_001530"
     CDS             complement(47..541)
                     /locus_tag="PXD71_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6291640.1"
                     /translation="MLSGLKIESFSKEFKRDCFDSESELLNQYLKTQVSQDIKRRITS
                     CFLAVNSEQTVVGYYTLASTSLSLSDLPITLQRKLPRYPTVPAVLLGRLAVDKRVKGL
                     GLGQALLGNALKRVLRADIAAYALIVEAKDESAVNFYRKFGFIDFENEARKLFFPLDR
                     VRLS"
     gene            complement(541..813)
                     /locus_tag="PXD71_001531"
     CDS             complement(541..813)
                     /locus_tag="PXD71_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDL6291641.1"
                     /translation="MATATARFEARMSQEVHMLLKRAASLEGRSLSDFVVGAALSAAR
                     KTVEQNDIIRLSVNDQELFAKALIDPPEPNLAMQKALALSSQILGE"
     gene            complement(972..1400)
                     /locus_tag="PXD71_001532"
     CDS             complement(972..1400)
                     /locus_tag="PXD71_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291642.1"
                     /translation="MRKLLVITALTLCTTPVFAADKNVIFSCTSTEGKPLTVKRVGND
                     YEYSYDKTTFKNPIKKAVTNDGSIIARGSGFTTYALELENDGLKYLVGFVQPNGNAKE
                     FIEPGATISQSKEQPSIGSVDCDTRKKSHYKFDVHLMNTL"
     gene            complement(1421..3427)
                     /locus_tag="PXD71_001533"
     CDS             complement(1421..3427)
                     /locus_tag="PXD71_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL6291643.1"
                     /translation="MKSQFKFKKTHLLVITVLFSSTSSISFADSITDKLEFSKSDSII
                     SDRVFYQIGGGTGYMAPPSRGNTKVVDLGIGWKANLTCGNFDIKTTIKNDLNGLKDGF
                     KDLMGNVIDSAKGAVASMPAMILQRANPQLYDLLTNGMYQGKLDFNRLKTSCEEMSKV
                     LADHTLGGKWGQAADLESYKQITSTQPDAKQAQKDLEDKKGKDGRVWVGGQRKGGQNQ
                     DPINVVGDVAKGGFNLLNKRNVLDSSPVSNCEGNLCQTWDTPQAAADFLTKIIGEQSI
                     STCKEDCGTKTSSKAGVGLSPEIEEEHIRTIVKLENVLNMDVPKSELLAELSSSSVPV
                     TRGLIESLRDDPDVELLSQRLSAEIATSKVMEKMLLARRAILAGMREPNVAQNADAQV
                     ELERVLNIIDREISQVKLEMDMQKMITGNTASVILQSKSEREALAGAHSLSQDSLDKR
                     VQGLENGNVALTDLETGSNINLSRRNIALSIPNIPNVAALIPTLPNRGGSGVGGNFPS
                     LAQISGSTMDQATGLLRNFEGFISKAQWDVNAHRVGYGSDTITRADGTVVKVTKDTVI
                     TKEDAERDLARRTAIFANNVRQELGDSHWNALPANAQAVLVSYAYNYGSLAKTKSVLD
                     AARRSAQTGDMTALATAVRNRQVDNNGINARRRNQEADYILGKN"
     gene            complement(3442..4383)
                     /locus_tag="PXD71_001534"
     CDS             complement(3442..4383)
                     /locus_tag="PXD71_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDL6291644.1"
                     /translation="MKRAFFSVLLSVSLAVPTTLYPQQVKASLNTASIAASSASTSCL
                     DYKVVGTCFWLFCTYFGCKVRTSTKIKHYLPEMVVSSYNQDGQTPWTELQSLNPGVKG
                     GEYRSPQRQYSQLTFKNADAFGHPQGAIGQLLGSTGYYCKSQTTPFIPYFLSGLDFFA
                     WRENMPEMFYPEALIPGMREVGRVGDSWGNIYPRSGWVTQAHDYKASATIVQRVADIV
                     TRTAQPHIYFPAAKKGGVGEWPPGPVEEGQARTHKWQMLYPQMSNTCSVFPNGGQTDT
                     YSSQISNQGDYAWALWRPYSCCKRRGQTFLYSIDWSH"
     gene            complement(4380..4823)
                     /locus_tag="PXD71_001535"
     CDS             complement(4380..4823)
                     /locus_tag="PXD71_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDL6291645.1"
                     /translation="MKASRFLGYFSIVTVVFSVVAQAQPNIIVYTTKMYPIQNSQLAS
                     QVYYLDQVENFEEEISRNLSKSPEIAEREIRAFFNSADWKAQEEQLKDAYQGVISGWQ
                     NGIKKVPAVFFNNLQSSPSVIYGMTDIQKALDEWHKWVQQHGESK"
     gene            5198..5551
                     /locus_tag="PXD71_001536"
     CDS             5198..5551
                     /locus_tag="PXD71_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291646.1"
                     /translation="MSQQTAGWDVIPFESSKTAKPSLFWKLGNKLGNIFVSAKHKAKS
                     KYVEVTKGINQRIAEEDEMRALELQMCLDEQAAEYEQEISRIKRKMFFRALFVAIIAF
                     LCGMATVFGTQLSLI"
     gene            5623..5868
                     /locus_tag="PXD71_001537"
     CDS             5623..5868
                     /locus_tag="PXD71_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6291647.1"
                     /translation="MSVHIKLSCVGDILPIKLNEWTPEHRALHHEISHKALEAWQNRD
                     KGTQSVRFMQQMPARQGLHFRFLSVSEQDGKLIVSRS"
     gene            5877..6119
                     /locus_tag="PXD71_001538"
     CDS             5877..6119
                     /locus_tag="PXD71_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291648.1"
                     /translation="MFGKLFYKMNPKVLAKIGEDFRKAGYLMGAGFVGMVISNDQIST
                     SEGFVLFLWGLYIWIIGHIALHHSEKILNSKQGEPK"
     gene            6383..7225
                     /locus_tag="PXD71_001539"
     CDS             6383..7225
                     /locus_tag="PXD71_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291649.1"
                     /translation="MSDSVLNTYFKHALVKAGFPDTLDMEYSLSHCQGDGVAFYGRLG
                     TDDLIQLFNHLNPQKRKQKMFANLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMEL
                     YAKTADQLVFFNDSDARRNWYFPTSKVDKYKALWDGFIEDLEQYIKDISREMESVGYQ
                     IIEASPYQTETVFKFNTEKYLVELKRKPSEFYSELVDWAFGDMCDFEAAIEQICQGQL
                     CYADFEATVTDKKSEIQLGLARLSGISYSPNDRSFSGYRQELIGEAIASARHNAARFA
                     YLHA"
     gene            7340..7753
                     /locus_tag="PXD71_001540"
     CDS             7340..7753
                     /locus_tag="PXD71_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6291650.1"
                     /translation="MKNEHNLEQLKYIWDNRNLENKVYQYLFHICIEPMKKGDWNRYT
                     IWLNFHGNLINVSDVVYRVVESQKLKDDIKIWYDKQYYCRPTKVETTGFISIFLHLFY
                     EGIARILLEQGMITETEAAEKSYNFRAANCFQRLL"
     gene            7767..7922
                     /locus_tag="PXD71_001541"
     CDS             7767..7922
                     /locus_tag="PXD71_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291651.1"
                     /translation="MEPRTVKVIYDDGDYFFTLINADEQTIRNYYAIGSSIGFDNTRK
                     IVAVEIL"
     gene            8002..8985
                     /locus_tag="PXD71_001542"
     CDS             8002..8985
                     /locus_tag="PXD71_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL6291652.1"
                     /translation="MNPQSNTQPKFDLYQEVTDKIIAALEQGTAPWLKPWDSVIPYGG
                     MPCNAISGRLYSGINVLLLWLSAAEHGYIQRKWITAKAAIEAGGHVRKGEKATLAVSY
                     RPYDREKTDDDGNLVLDDDGNPVIEHLASIKKHCLFNIEQCEKLPQDWYETVETPEQL
                     TDENQYAIFEEIRLMLKGMDLQVMVKPSNQAYYHPRNDHIVMPEMKQFHSPQKFHSVL
                     LHEMTHATGHQSRLARDGIVSGKARFGNKVYAFEELVAEMGCAFLCSHLGFADVPQNA
                     SYIESWIKVLREDKRAIFRASGKAREACQYMLDALNIAQLADKYEVFEEAA"
BASE COUNT         2879 a         2001 c         1929 g         3083 t
ORIGIN      
        1 tcctgcctaa ccaataacca aaattatttt tcataatcac tctccattaa gataatctca
       61 ccctatctag tggaaaaaat aatttcctag cctcattttc aaagtcaata aatccaaatt
      121 tacgataaaa attgaccgca ctttcatctt ttgcctcaac aattaatgcg taagctgcta
      181 tatctgcacg aagtaccctt ttaagtgcat tacctaatag agcttgccct aaccctaatc
      241 cctttacacg cttatctact gctaatcgac caagtaggac ggcaggtacc gtaggatatc
      301 ttggtaattt cctctgtaat gtaattggta aatcagataa tgaaagactt gttgaagcca
      361 gtgtgtaata tcctactact gtctgttcag aattaacagc taaaaaacaa gaagtaatac
      421 gtcttttaat atcttgtgaa acctgagttt tgagatattg atttaataat tcagattcac
      481 tatcaaaaca atctcgttta aattccttgc taaatgactc tattttcaat cccgaaagca
      541 ttattccccc agaatctgtg agcttaatgc caaggctttt tgcattgcca aattaggttc
      601 aggaggatca atcaatgctt tagcaaaaag ttcttggtca ttcacagata atcgaataat
      661 atcattttgc tcaactgttt ttcttgctgc agatagtgca gctccaacaa caaaatcact
      721 aagactacgc ccttcaagag aagcggctcg ttttaacagc atatgaactt cttggctcat
      781 tctagcttca aatcgtgcgg ttgcagttgc catatatttt ctccttgaat tatttagttt
      841 taattgtacg tatttatacc gtacaaatca agcatgacaa taaaaaaatc ccttagattt
      901 tcaaacctaa gggatttacc taccgatctt tgtgtttttt aacgtgggct ggccctagtg
      961 ccatacaggc attataaagt attcatcaaa tgaacatcaa acttataatg gcttttttta
     1021 cgagtatcac aatcaacaga gccgatactt ggttgttctt tactttggct aatcgttgct
     1081 cccggctcaa taaattcttt tgcattgcca tttggctgaa caaagccgac caaatacttc
     1141 aatccatcat tttccagctc taatgcatac gttgtaaaac ctgaacctct ggcgataatt
     1201 gagccgtcat tagtgactgc ctttttaatc ggatttttga aggttgtttt gtcatacgaa
     1261 tattcgtaat catttcctac ccgtttaaca gtaagcggtt ttccctccgt gctggtacag
     1321 gaaaatatta cgtttttatc tgctgcaaat actggggtcg tgcagagtgt aagagcagta
     1381 attactaata atttacgcat aaatgtaact ccttataagg ttaattttta ccaagaatat
     1441 aatcagcttc ctgattccgg cggcgtgcat tgataccgtt attatcaacc tggcgatttc
     1501 tgacagcagt tgccaaagca gtcatatcac cagtttgagc tgatcgtcta gctgcgtcaa
     1561 gcacactttt tgttttcgct agtgagccgt aattatatgc ataagacacc aaaacagcct
     1621 gagcgttcgc cggtaatgca ttccaatgtg aatcacctaa ctcttgtctc acattgttcg
     1681 caaagatagc agttctgcga gctaaatcac gttcagcatc ttctttggta atcacagtat
     1741 ccttggtcac tttaactacg gtaccgtctg cccgtgtaat cgtatcggaa ccatatccta
     1801 cacgatgggc attcacatcc cattgagcct tggagataaa gccttcaaaa ttacgcagta
     1861 accctgttgc ctgatccatc gttgaaccgc tgatttgtgc aagtgatggg aaatttccgc
     1921 ctacacctga accacctcta tttggtagag tcgggatgag agctgctaca ttcggaatat
     1981 ttgggatact caatgcaata tttctacgag agaggttaat attagagcct gtctctaaat
     2041 cagttaatgc aacattaccg ttttcaagtc cttgaacacg cttatccaga ctatcttggg
     2101 ataaactatg agcaccagca agagcttccc gttcggattt actttgcaga atcaccgaag
     2161 ccgtattgcc ggtgatcatt ttttgcatat ccatttccag ttttacttgg gaaatttcac
     2221 ggtcaataat attgagtact ctttccagtt ctacttgagc atccgcattt tgagcaacat
     2281 tcggctctcg cataccagct aggattgctc ggcgagccag tagcattttt tccattactt
     2341 tactggtcgc aatttcagcg gataaacgtt ggctgagaag ttcaacatct ggatcatcac
     2401 ggaggctttc aatcaagcca cgagtgacag gtacactact tgagcttaat tcagctaata
     2461 gctcggattt cggcacatcc atattcagta cattttctaa tttaacaatt gtacgaatat
     2521 gttcttcttc aatttccgga cttaaaccaa caccggcttt cgaactggtt ttagtaccac
     2581 aatcctcctt acaggttgaa attgattgct caccaataat cttagttaag aagtcggcag
     2641 cagcttgagg tgtatcccaa gtctgacaaa gattaccttc acaatttgaa accgggctgc
     2701 tatctaagac attccgctta ttgagtaaat taaatccgcc tttcgctaca tcaccaacga
     2761 cattaattgg atcttggttt tgcccacctt tacgttgccc accaacccaa actctcccat
     2821 ctttcccttt tttatcttct aggtcttttt gggcttgctt cgcatcaggt tgtgtcgagg
     2881 taatttgctt atagctctct aaatctgctg cttgccccca ttttcctcct agtgtgtgat
     2941 ctgccagaac tttagacatt tcctcacagg aagtttttag acggttgaaa tccagcttac
     3001 cttggtacat accatttgtc agcaaatcgt agagttgagg gttggcccgt tgcaatatca
     3061 ttgcaggcat actggcaacc gcacctttag ctgaatcaat gacattcccc attaaatctt
     3121 taaagccatc tttaaggccg tttaaatcat ttttaatagt tgttttaatg tcaaaattgc
     3181 cacaggttaa attcgctttc caaccgatgc ctaaatcaac gactttggta ttaccacgac
     3241 taggtggagc catataacct gttcctccgc cgatttggta aaagacacga tctgaaataa
     3301 tcgaatccga cttactaaat tccagtttat cagtgattga gtcagcaaaa gagatagagg
     3361 aagtactgga aaaaagtact gttattacta atagatgtgt tttcttgaat ttaaattgtg
     3421 atttcattgc aagacctcgt tttagtgtga ccaatcaata ctgtaaagga atgtttgccc
     3481 tcggcgttta caacaactat aaggtcgcca tagtgcccaa gcataatcac cttgatttga
     3541 aatctgagag gaataggtgt ctgtttgccc cccatttgga aaaacagagc aggtatttga
     3601 catttgagga taaagcattt gccatttatg tgttctggct tgtccttctt cgacaggtcc
     3661 tggtggccat tctccaacac cacctttttt cgctgccggg aaataaatat gtggttgagc
     3721 agttcgggtc acgatgtctg caactctctg cacaatggtt gcagaggctt tataatcgtg
     3781 agcttgtgtt acccaaccgg aacgtgggta aatatttccc cagctatcgc ctacacggcc
     3841 aacttcccgc attcccggaa tcagagcttc cggataaaac atttccggca tattttcacg
     3901 ccaagcaaag aaatctaatc cacttagaaa atagggtata aatggtgtgg tttgactttt
     3961 acaatagtag cctgtacttc ctaataactg accaattgct ccttggggat gcccgaaagc
     4021 atctgcattt ttgaaggtta gttgagagta ttgacgttgc ggagaacgat attctccgcc
     4081 ttttacgcca ggattgagag attgtaattc tgtccacgga gtttgcccat cctggttata
     4141 agatgaaacg accatttccg gtaaataatg cttaattttg gttgatgtac gaaccttgca
     4201 tccaaagtag gtgcaaaata accagaagca cgttccgacg actttatagt ctaaacagct
     4261 tgttgaggca cttgatgcag caatcgacgc cgtattcaat gatgctttta cctgttgagg
     4321 atataaggtt gttggtactg ctaaacttac agagagaagt accgaaaaaa acgctctttt
     4381 cattttgatt caccgtgttg ttgaacccat ttatgccatt catctagtgc tttttgtata
     4441 tcagtcatac cataaatcac gctcggagag gattgcagat tattaaaaaa caccgcaggc
     4501 acttttttaa ttccattttg ccatccactg atcacccctt gataagcatc ttttaattgc
     4561 tcttcttggg ctttccaatc tgctgaatta aagaatgctc gtatttctcg ttcggcaatc
     4621 tcgggggatt tactgagatt tcgactgatt tcctcttcaa aattctctac ttgatcaaga
     4681 taataaactt gggaggcaag ttgtgaattt tgaatggggt acatctttgt tgtatagaca
     4741 atgatattcg gctgtgcttg tgccactaca gaaaacacta ctgttactat gctgaaatat
     4801 cctagaaatc tacttgcttt catcactctc tttccgaata aataagcaga aaattagcga
     4861 gatttttaca tagaggatag gaaaaactct tttcaaaaaa gagatatttt tttgagcaaa
     4921 aaatggttga ttttataaat ggatttcgta tgatatggaa tattaagatg ctgtggaaac
     4981 agcccttaaa agtcgcataa ctcttgagaa gcggcggcta tccatcgagg tagttgagtt
     5041 atttacccaa aatgtctccc agcattctga aagggaacgc tgcaaatttg caggaatgcc
     5101 acctaaacac ctaccttccg agggatattt caattccctt ggaattgcga tctcctgagg
     5161 aattttattt atttaacttt ttataggaga actcgctatg agtcaacaaa ccgcaggatg
     5221 ggatgtgatc ccatttgaat cgtcaaaaac cgcaaaacct tctctttttt ggaaacttgg
     5281 caataagctc ggtaatattt tcgtttcggc gaaacataaa gctaaaagca aatatgtcga
     5341 ggtaaccaaa ggcatcaatc aacgaattgc tgaagaagat gaaatgcgag ccttagaatt
     5401 gcaaatgtgc ttagatgagc aagcagctga atatgagcag gaaatttctc gcattaagcg
     5461 taagatgttt ttcagggctt tattcgttgc gattattgcc tttttatgtg gtatggctac
     5521 cgtttttggt actcagctct ctttaatcta atttaactca cccatcgggg aaatgctatt
     5581 tcccttgtgg taatacattt ccccttaatt ttggagaaat gtatgtcagt tcatatcaaa
     5641 ctctcctgtg ttggagatat tttgccaatc aaactcaatg aatggacacc ggaacatcga
     5701 gccttacatc acgaaattag ccataaagct ctcgaagcct ggcaaaatcg agataaaggc
     5761 actcaaagcg tgcgatttat gcaacaaatg ccagcccgac aaggactgca tttccgcttt
     5821 ctgagtgtat cggagcaaga cggcaagctc attgtcagtc gttcatagtt ggttcaatgt
     5881 ttggtaagct tttttacaaa atgaatccca aagtactagc taaaatcgga gaagatttta
     5941 gaaaagctgg ttatcttatg ggagctggtt ttgtcggaat ggtaatttcc aatgatcaga
     6001 tttccacatc ggaaggattt gttctttttt tgtggggatt gtatatttgg attatcgggc
     6061 atatcgcact acatcattct gagaaaatac ttaattcaaa gcaaggagaa ccgaaatgag
     6121 tccgtacttt ttaggtgctg ctattggaac agcgttattg gcaacaattt ttattgttct
     6181 agtaacttgg gcaaatcgag atattttgtt tacaaaatca gataagccac agtctaaata
     6241 gcaaaactcc attctaaccc ataggggaac aagttccccg aggagcttgt tctccgtttt
     6301 accttccttt taccctatcg ggaaatactt tcccgaattg ggcttaagtg tttcccatta
     6361 ttttttattt tggagaaaca ttatgtcaga ttcagtatta aacacttatt ttaaacacgc
     6421 tcttgtcaaa gctggttttc cggatacctt agatatggag tattcactca gccattgtca
     6481 aggagatgga gtcgcatttt atggtcggct tggtacagat gatctaatcc agcttttcaa
     6541 tcatctcaat ccacagaaac gtaaacagaa aatgtttgct aatttgctaa cgcacattga
     6601 atcttgggaa acccacgatg atattggttt tgaaattata cgaaatcgct ttgggtatcg
     6661 ttactcgcat tggaatacga tggagcttta tgctaaaact gcagatcagt tagtgttttt
     6721 caatgatagt gatgctcgaa gaaactggta ttttcctact tcaaaagtgg ataaatacaa
     6781 agcattgtgg gacggattta tcgaagattt ggagcagtac atcaaagata tttcccgtga
     6841 aatggaatcg gtcggttatc aaattattga agcctcgccg tatcaaacgg aaaccgtttt
     6901 caaatttaat acggaaaaat atctagtaga actgaaacgt aaaccttctg agttttattc
     6961 agagcttgtt gattgggcat ttggtgatat gtgtgatttt gaagcagcta tagagcagat
     7021 ttgtcaaggg caactctgtt atgctgattt tgaagcaact gttaccgata agaaaagtga
     7081 aattcagtta ggtcttgctc gcttgagtgg aataagttac tcgccgaatg accgttcgtt
     7141 tagtggttat cgacaagagc ttattggtga ggcgattgcc tctgctcgcc ataatgcagc
     7201 acgttttgcc tacttgcacg cctaatttgc agatttttac tttaatttga ccgcttgtag
     7261 tggttaattt tcaccccgtg gggaaatatt ttccctattg ggcaaaatgt ttctccactt
     7321 tttaatttgg agaaatgtta tgaaaaatga acacaattta gagcaactta aatatatttg
     7381 ggataaccgc aaccttgaaa ataaagtcta tcaatatctg tttcacatct gcattgagcc
     7441 gatgaaaaaa ggagattgga atcgttatac gatttggctt aattttcacg gtaacttaat
     7501 caacgtgtca gacgttgtat atcgagttgt ggaatctcaa aagctcaaag atgacatcaa
     7561 aatttggtat gacaagcaat actattgccg tccaaccaaa gttgaaacaa caggttttat
     7621 ttccattttt ctgcacctgt tttatgaggg catagctcgt attttgctgg aacagggaat
     7681 gattactgaa actgaagcag ccgagaaaag ttacaacttc agagctgcaa attgttttca
     7741 acgtttgctt taatggaggt gagataatgg aacctcgcac tgttaaagtt atttacgatg
     7801 atggcgatta tttctttacc ctaatcaatg ccgatgaaca gactattcgt aattactacg
     7861 ctataggcag ttcaatcggt tttgataaca caaggaaaat tgtcgctgtt gaaattttat
     7921 aaactaccca tcggggaaac gacttcccca tagggcgata gtgtttcccc atttttctac
     7981 ccgaactttg gagaaacact tatgaaccct caatcaaaca cacagcctaa atttgattta
     8041 tatcaagaag tgaccgataa aatcatcgcc gctttagagc aaggaacggc tccttggctt
     8101 aaaccttggg atagcgttat tccttatggt ggtatgccgt gtaatgccat atccggaaga
     8161 ttgtattccg gaatcaatgt tttgttactt tggttgtctg ccgcagaaca cggctatatc
     8221 caacgcaagt ggatcactgc aaaagcagct attgaagccg gtggacacgt tcggaaaggt
     8281 gaaaaagcaa cccttgcggt cagctatcgc ccgtatgatc gagaaaaaac cgatgatgat
     8341 ggcaaccttg tcctcgatga cgatggtaac ccggtaatcg aacatctcgc ttcgattaaa
     8401 aagcactgcc tattcaatat tgaacagtgc gaaaaactgc cacaggactg gtatgaaacg
     8461 gttgaaaccc cagaacagct gactgacgaa aatcagtatg ctatttttga ggagattcgt
     8521 ttgatgttga aaggtatgga tttgcaagtg atggtaaaac caagcaatca agcttactac
     8581 cacccacgca atgaccatat cgtgatgccg gaaatgaagc agtttcattc accacaaaaa
     8641 ttccatagcg tgctgctaca tgaaatgact cacgcaacag ggcatcaatc tcgccttgct
     8701 cgtgatggta ttgtttccgg aaaagctcgc tttggcaata aagtttatgc ttttgaagaa
     8761 ttggtcgctg aaatgggctg tgcattcttg tgttctcacc taggctttgc tgacgtgcca
     8821 caaaatgcca gctacattga aagttggatc aaggtgttaa gagaggataa acgggcaatt
     8881 ttcagggctt cagggaaagc tcgggaagcc tgtcaatata tgctggatgc cttaaacatc
     8941 gcacagctgg ctgataaata cgaagtattt gaagaagccg cttaataaat ttgccgaaag
     9001 gcatttcccg tggggagata ctctcccttc ggggcattgt gtctcccttt ttattcactt
     9061 aacgagagga ctcaatgctc aatctaaact tatttaaacg acccattgaa atttcgacac
     9121 ttgaatcttg gtcaaaattc tgtgatgatg ttggtaaagt agcttttgtt gcagtacctg
     9181 ctatgctgta tagtaattac gagttttgga ttaagataag cagtataatt ggtctattta
     9241 tagctaccta tttctgttta gttatcggta gaaatatacg ccaatttatt gagaaggaga
     9301 aatcaaaatg ggattaatga tcggcatttg gattattgct tttattgcat ttgggttggc
     9361 catatggaca agccatattc taaaaaagca agatgaacgc cttaaacagc taaaaaatag
     9421 ctaactttca aaatacggca cattttgtgc cgtatttctt ttgtactggt ggcaaatata
     9481 agattactat catcagtata ttgtaatatc tcaactcaac agcattagtc aaaaatcctc
     9541 caacatattc agagagtttt tcctactgag ttctcttatt ttttggttca atactctccc
     9601 tttataaatt aaatttggtt cagataaaag ccgctcaatg gcaaaaggat aaaggagccc
     9661 ttacgggcaa gggtaatccc ctaaggggga ataagggtaa cctttccaag gggaaaggtc
     9721 ttacccaaaa gggcaaaggg ctaaacggct ttctttcaac gcaaacatca tctttttcgg
     9781 tctatttcaa tgcactcaat tctcaaaaaa attcttggca aaagtaaatc ctcgtctcaa
     9841 agttcggtta aatctgacaa tgttttctct attgagcagg atagtgaggg ct
//
LOCUS       DANLXS010000085         9886 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.085, whole genome shotgun sequence.
ACCESSION   DANLXS010000085 DANLXS010000000
VERSION     DANLXS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.085"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            9..3167
                     /locus_tag="PXD71_001543"
     CDS             9..3167
                     /locus_tag="PXD71_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL6291653.1"
                     /translation="MITINENTIEQSAIATLQSLGWDYTYGKKILAGLEHEWRERTAE
                     VILKPLLAQAIAKFNPNLPACEVENVVAQVCRADSGDLAERNRQAYDWLRNGVKITYQ
                     LYGEQVSDVVQLIDFQRPENNDFRIVNQLDISGKKGKRIPDLIGFVNGLPLVVFELKN
                     PLKENADIGKAFAQLQTYKDEISDLFVFNQALVISDGIVARIGSLTADFDRFTPWRVV
                     DEKNQSKRIVFEDELTALLQGVMTPKNLLDYVQNFVVFERDGKNRLIKKIGAYHQFYG
                     VNEAVDCTLLAATGNRKIGVFWHTQGSGKSLSMLFYAGKVLSQSSLKNPTLVLVTDRN
                     DLDGQLYATFCGGEALLKQTPIQADGRDELRSALASRSAGGVIFTTIQKFGLMEGELA
                     HPVLNERENIIVITDEAHRSQYGFSQKINHKGEYREGYAKHLRSALPNASFIGFTGTP
                     IELDDKDTQEVFGKYVSIYDFEDAVEDGATVPIIYEPRQISLGESGEFSKVMEEAQQL
                     IDDDENSYNFRLREKLHSVDSRLQKMAEDIIAHYDERTKQQDGKAMVVVMSRAICVKL
                     YEKITALRPEWHSNDVLQGSIKIVMTSNASDPAEWQKHNQDKKTLEKRFKDPDDPLNI
                     VIVRDMWLTGFDAPCCNTMYIDKPMSGHNLMQAIARVNRVFRNKSRENGGLIVDYVGL
                     TDELEKAMKQYTNAGGKEKPVRDISAVLEKMVEHITVIHGQFATPIDGQAVDIAKMLQ
                     ISEPPKLLNAILTSANHILALDRIQPPDNTAKDKTPRKNAFLQSVRLAKKGYALCGAL
                     KAVEPYKQELAFYDAVRATIIKNSTAPRNSSSENDRLLQLTALMNRAVQSDGVVDLFD
                     LLKKDRPNINLLSDEFLETVKNSPTKDLWLSAMERYLASQLREQSGANLATKKAFEQK
                     LKEAMNQYHNHNLSVLEILEELIALAKEFEARQKRGEALGLSPAEMAFYDALARNESA
                     VREMGDEVLMKLAKDITDKLRKSVTVDWQYKDSVRAKMRTLIRIALRTYKYPPDLQAE
                     AIEFVLQQAEEIAGEFAITETA"
     gene            complement(3201..6092)
                     /gene="glnE"
                     /locus_tag="PXD71_001544"
     CDS             complement(3201..6092)
                     /gene="glnE"
                     /locus_tag="PXD71_001544"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDL6291654.1"
                     /translation="MLTTLIPLSQLNLPPNLQNSEQIQPLVTAISLSNFVHQTLQKQP
                     DLLAEWLAKFPTPDDCCNYTERLAKILARVENEEMLGRVLRQFRHRELARLSFIQSNK
                     LATVELVFQHLSDLAESLILAARDWLFQRCCAEYGTPKNTLGETQELLILGMGKLGGR
                     ELNFSSDIDLIFTYPDIGETEGGRKSIENSKFFTRMGQRLIKVLDEITADGFVYRTDM
                     RLRPFGDSGALVLSFTAMEDYYQEQGRDWERYAMIKAKILGEDSQNLNHRYLKQMLRP
                     FVYRRYLDFSAIQSLREMKEKISREVVRRNLTDNIKFGAGGIREVEFIAQTFQMIRGG
                     RDKILQERSLLKVLPRLAELNLLTQTQVETLHNAYIFYRQIENVLQAIDDKQTQTLPT
                     DEASQARLVFACQSYYQQDLYSEKTHWVEHSFNDWQQFMAVLSQYQQAVRQIFNEIIG
                     EEETQANCNPVNEKLAEWKDILHYNIRLEDLSAVLKGYPKVAENDYTEIFRHFSTTFQ
                     DWVKRPIGVRGREVLRNLMPRIADSIFKGEDHLLLLPRVLNIVDKITTRTTYLELMLE
                     KEQILPQLLALCSKSVMIAEQIARYPMLLDELMSHRGLTDVQAFEKYQSALQDYLIRI
                     PEEDEEALIDGLRQFKQTQILRIAAADILGVLLVMKISDHLTYLAEAIINAVVNMAWK
                     QVSQRFGVPEHLEADEKGFAVIGYGKLGGIELGYNSDLDLVFLHNAPEDSQTVGGKKE
                     ISSHQFYLKLAQKINSIFNLNTSAGVLYEVDMRLRPSGEAGLLVSTFNAYEHYQKNEA
                     WTWESQALVRTRCVFGAENLKQAFEKIRQSTLAQPRASGQLRQEICEMRQKMYQHLSS
                     HSAEQFHIKQDQGGITDIEFIAQYLVLAHSHQHPKMAVWSDNVRIFDSAVECGILSSE
                     QSEQLQHCYTALRNKIHHLNLLRKDSVVDASEFTTERAFVREMWQRLLA"
     gene            complement(6264..6545)
                     /locus_tag="PXD71_001545"
     CDS             complement(6264..6545)
                     /locus_tag="PXD71_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDL6291655.1"
                     /translation="MYYVIFAQDNPNMLEKRLEVRPAHLARLEKLQAEGRLLIAGPNP
                     TEDGSSVTGSTVIAEFNSLEEAQQWASQDPYVEAGVYSDVIIKPFKRVF"
     gene            complement(6547..7011)
                     /gene="yciA"
                     /locus_tag="PXD71_001546"
     CDS             complement(6547..7011)
                     /gene="yciA"
                     /locus_tag="PXD71_001546"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDL6291656.1"
                     /translation="MKDKPYGKAPEGKLILRTLAMPSDTNANGDIFGGWIMSQMDLGG
                     AILAKELAKGRVVTVTVDKMIFHRPISVGDVVCCYGKLMHIGRSSMQVKVEVFIKQVY
                     EGNRERYLVTEALFTYVAIDKEGKPRAVPRENNPELDEALSLIDLTPEKQGE"
     gene            complement(7001..7558)
                     /locus_tag="PXD71_001547"
     CDS             complement(7001..7558)
                     /locus_tag="PXD71_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDL6291657.1"
                     /translation="MKQLLEFIPLILFFVVYKMSGIQMASIALVIATIVQMIALKMLY
                     GKIEKQQIIMGGSVVFFGLLSAYFNEVKYLQWKVTIVYALFALVLLVSQFVFKTPLIK
                     KLLGKEIQLPEAVWNKINFGWAVFFLICMLLNIYISQYLSEEIWVDFKSFGIIGLTLV
                     ATVITGFVLYPYLKDAEKQEQKNER"
     gene            complement(7567..8376)
                     /locus_tag="PXD71_001548"
     CDS             complement(7567..8376)
                     /locus_tag="PXD71_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6291658.1"
                     /translation="MAQALLYFTTKYLQSIEGYFMSIKFVDLLQQSWNFMRNQQAFSL
                     FAIVTIVVVQLVFILVSPNSPELMAESQPQQLQIDASKMVSILLPTILLGVVNLLINV
                     LMIFNIQSINDGNYRQFFQNAGNALKHFLPVLLLQFVMVLPLSLGASFAMASPETVII
                     ALPVLVVGFYFFIKLSLVIYAYLLEKPQKSLSESIKFTLQLSRGKMLPLILFCVISYL
                     LPGMLSRLFTVFGNDFIGIFITIVLSAVINVFMAIFSFRFYQVYRQMPAVR"
     gene            8447..8884
                     /locus_tag="PXD71_001549"
     CDS             8447..8884
                     /locus_tag="PXD71_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDL6291659.1"
                     /translation="MLSLFDAGQRYLNTLPNQKKLANFMPDYHIIRLVKFSSKVMPAF
                     ACFALVWQYCFTDPTQSILANSVLTAVFAISIPFQGLYWLGKRARSPLSLSLLEWYES
                     LRQKLISAQVKLEETQAVPSFQDFANLLKLAEETWGDEYFDEL"
     gene            complement(9169..9591)
                     /locus_tag="PXD71_001550"
                     /pseudo
     CDS             complement(9169..9591)
                     /locus_tag="PXD71_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(9721..9886)
                     /locus_tag="PXD71_001551"
                     /pseudo
     CDS             complement(9721..9886)
                     /locus_tag="PXD71_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019518307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         2903 a         2119 c         2066 g         2798 t
ORIGIN      
        1 gagttgtgat gattaccatc aacgaaaaca ccattgaaca atccgccatt gcaactttgc
       61 aaagcttggg ctgggactac acctacggca aaaaaatttt ggcaggttta gaacacgaat
      121 ggcgggagcg aaccgctgag gtgattttaa agccgctttt ggcgcaagcg attgcaaaat
      181 tcaacccgaa tttgcccgct tgtgaggtgg aaaatgtggt ggcacaggtg tgccgtgccg
      241 acagtggcga tttggcggag cgtaatcgtc aggcttatga ttggctaaga aatggggtca
      301 aaatcactta tcagttgtac ggtgagcagg tgtctgatgt ggtgcagctg attgattttc
      361 agcgtccaga aaataacgat tttcgcattg tcaatcagct ggatattagc ggcaaaaaag
      421 gcaaacgcat tccggatttg attggctttg ttaatggctt gccgctggtg gtgtttgagc
      481 tgaaaaatcc gctcaaagaa aatgccgaca ttggcaaggc gtttgcccaa ctgcaaacct
      541 ataaagatga aatttctgat ttgtttgtgt ttaatcaggc actcgtgatt tcagacggca
      601 ttgtcgcccg catcggttcg ctgaccgctg atttcgaccg ttttacccct tggcgtgtgg
      661 ttgatgaaaa aaatcagagc aaacgcattg tgtttgaaga tgaactcacc gccctgctgc
      721 aaggcgtgat gacacccaaa aatctcttgg attatgtgca gaattttgtg gtgtttgaac
      781 gggacggcaa aaaccgctta attaagaaaa tcggagcata tcatcagttt tatggcgtga
      841 atgaagcggt ggattgcacc ttgcttgccg ccacaggcaa ccgcaaaatc ggcgtgtttt
      901 ggcatacgca aggttcgggc aaatcgcttt cgatgctgtt ttatgcaggt aaagtattaa
      961 gccaaagcag cctgaaaaat ccaactttgg tgttggtaac cgaccgcaac gatttggacg
     1021 gtcagcttta cgccactttt tgcggtggcg aggcactgct caaacaaacg ccaatccaag
     1081 ccgatgggcg agacgaactc cgctctgccc ttgccagtcg ttcggcaggc ggtgtgattt
     1141 ttaccaccat tcaaaaattc ggcttaatgg agggtgagct tgcccaccct gtattaaacg
     1201 agcgggaaaa catcattgtg attaccgatg aagcacaccg ctcacaatat ggttttagcc
     1261 aaaaaatcaa ccacaaaggc gagtatcgtg agggttatgc caagcattta cgcagtgcat
     1321 tgccgaatgc ctcttttatt ggctttactg gcacaccgat tgaacttgat gacaaagaca
     1381 cccaagaagt cttcggtaaa tatgtctcga tttatgattt tgaagatgcg gtggaagatg
     1441 gggcaaccgt gccgattatt tatgagccac gccaaatcag cttaggcgaa agtggcgagt
     1501 tttccaaagt gatggaagag gcacaacaac tgattgatga cgatgaaaac agctataact
     1561 tccgcctgcg tgaaaaactg cacagcgtgg atagccgttt gcaaaaaatg gcggaagata
     1621 ttattgccca ttacgatgag cgtaccaaac agcaagacgg caaggcgatg gttgtggtga
     1681 tgagccgtgc catttgcgtg aaattgtacg aaaaaatcac cgcacttcgt ccagaatggc
     1741 actcaaacga tgtgcttcaa ggcagcatta aaattgtgat gacaagtaat gccagcgacc
     1801 ctgccgagtg gcaaaaacac aatcaggata agaaaacctt agaaaaacgc tttaaagacc
     1861 cagacgatcc gctcaacatc gtgattgtgc gggatatgtg gctgacaggc tttgatgcgc
     1921 cctgctgtaa cacaatgtac attgataaac caatgagcgg acacaacctg atgcaggcaa
     1981 tcgctcgggt aaatcgggta tttcgcaaca aaagccgtga aaatggcggc ttgattgtgg
     2041 attatgtggg cttgaccgat gagctggaaa aagcgatgaa gcagtacacc aacgcaggcg
     2101 gtaaagaaaa gccggtgcgg gacatttcag cggtgctgga aaaaatggtg gaacatatca
     2161 cggtcattca tgggcaattt gccacaccga ttgacggaca agcggttgat attgccaaaa
     2221 tgttgcaaat cagcgaaccg cctaaactgc tcaatgcgat tttgacatca gccaaccata
     2281 ttcttgccct agaccgcatt cagccacctg ataacactgc caaagacaaa accccacgca
     2341 aaaacgcctt tttgcaatcg gtacgcttgg cgaaaaaagg ttatgctttg tgtggggcat
     2401 taaaagcagt tgaaccctat aaacaggaac tggcgtttta tgatgcagtg cgtgccacca
     2461 ttatcaaaaa cagcaccgct cctcgcaatt cttcgagcga aaatgaccgc ttgttgcagc
     2521 ttaccgcctt gatgaatcgt gcggtacagt cggatggtgt ggtggattta tttgatttgc
     2581 tgaaaaaaga ccgcccaaac atcaacctgc tttctgatga gtttttggaa acagtaaaaa
     2641 acagcccaac caaagatttg tggctatcag caatggagcg ttatcttgcc tcacaactgc
     2701 gagagcaaag cggtgccaat cttgccacaa aaaaagcatt tgaacagaaa ctcaaagagg
     2761 cgatgaacca ataccacaac cacaatttaa gcgtattgga aattttagaa gagctgatcg
     2821 ccctcgccaa agagtttgaa gcccgccaaa aacgagggga agcgttggga ctaagccctg
     2881 cggaaatggc gttttatgat gctttggcac gtaatgaaag tgcggtgcgg gaaatgggcg
     2941 atgaggtatt gatgaaactt gccaaagaca ttaccgataa attacgcaaa tcggtcaccg
     3001 tagattggca atataaagac tcggttcgtg ccaaaatgcg aaccttaatc cgcattgctc
     3061 tgcgtaccta taaatacccg cccgatttac aggcggaagc gattgagttt gtgttgcaac
     3121 aagcggaaga gatcgccgga gagttcgcta tcaccgaaac ggcgtaatct atattagggc
     3181 gtgcaatgta cgccctaaat ttatgctaat aacctctgcc acatttcccg cacaaatgct
     3241 cgctcggtgg taaattcact tgcgtccacc acgctgtctt tgcgtaataa gtttaggtgg
     3301 tggattttgt tacgcagtgc ggtgtagcaa tgttgtagct gttcgctttg ttcggaactt
     3361 aaaataccgc attctactgc gctgtcgaaa attcggacat tatccgacca tactgccatt
     3421 ttcgggtgtt ggtggctgtg ggctaatacc aaatattggg cgataaattc aatatcggta
     3481 atacctcctt gatcttgttt aatatgaaat tgctcggctg aatgcgagct taaatgttgg
     3541 tacattttct gtcgcatttc gcaaatttct tggcgtaatt gaccgcttgc acgaggctga
     3601 gcaagcgtgc tttggcggat tttttcaaag gcttgtttga ggttttccgc cccaaataca
     3661 catcttgtcc gcacgagggc ttggctttcc cacgtccacg cttcattttt ttgataatgc
     3721 tcgtaggcat tgaaagtgct gactaaaagc ccagcctcgc ctgatgggcg aagtcgcata
     3781 tccacttcat atagtacgcc ggcactggta ttaaggttaa aaatcgagtt aattttttga
     3841 gccaatttta agtaaaattg atggcttgaa atctcttttt taccgccaac agtttggcta
     3901 tcctctgggg cattatgcaa gaaaaccaag tcgagatctg aattatagcc cagttcaatc
     3961 ccgcctagct tgccgtagcc aataactgca aacccttttt catcagcctc taaatgttcc
     4021 ggcacaccaa agcgttggct gacttgcttc cacgccatat ttaccaccgc attaataatc
     4081 gcttctgcta agtaagtaag atgatcgctg atcttcatta ccagcagcac gcctaaaata
     4141 tcagcagcgg cgatgcgtaa aatttgggtt tgtttgaact gacgtaagcc atcaatcagt
     4201 gcttcttcat cttcttcagg aatgcggatt aaataatcct gcaaggcaga ttgatatttt
     4261 tcaaaagcct gcacgtcggt taagcctcta tggctcatca gctcatcaag tagcattgga
     4321 tatcgagcta tctgttccgc aatcataacc gacttactac acaatgctaa aagttgaggc
     4381 agaatctgct ctttttcgag cattaattcc aaataggtag ttcgggtggt gattttatcg
     4441 acaatattca acacgcgagg caataacaat aaatgatctt cacctttaaa aatgctgtcg
     4501 gcaattctag gcattaaatt gcgtagcacc tctcgccctc ttaccccaat cgggcgtttt
     4561 acccaatctt gaaaggtagt agaaaagtga cggaaaatct ccgtataatc attttctgca
     4621 acctttggat aaccttttag cactgcggat aaatcttcaa ggcgaatgtt atagtgcaaa
     4681 atgtctttcc attcggctaa tttttcatta accggattgc agttggcttg tgtttcctct
     4741 tcaccgataa tttcattaaa aatctggcgt actgcttgtt gatattggct caatactgcc
     4801 ataaattgtt gccaatcgtt aaaggaatgc tccacccaat gcgttttttc gctatataga
     4861 tcttgctgat aatagctttg gcaagcaaat acaaggcggg cttgcgaggc ttcatcagtg
     4921 ggtagggttt gagtttgttt atcgtcaatc gcttgtaata cattttcaat ttgacggtaa
     4981 aaaatatagg cattgtggag cgtttccacc tgcgtttggg ttaataaatt taattctgcc
     5041 aagcgtggta gcacttttaa caggctacgt tcttgtaaaa ttttatctcg cccgccacgg
     5101 atcatttgga aagtttgggc gataaactcc acttcccgaa tcccaccggc accaaattta
     5161 atattatcgg ttaaattacg gcgaaccact tcacggctga ttttttcttt catttcacgc
     5221 aacgattgga ttgcactgaa atccagataa cggcggtaaa caaaagggcg gagcatttgc
     5281 tttaaatagc gatgatttaa attttggcta tcctcgccta aaatttttgc tttaatcatc
     5341 gcataacgtt cccaatcacg cccttgctct tggtaataat cttccattgc agtaaaactc
     5401 agcaccaagg ctccgctatc accaaagggg cgaagtcgca tatcggtacg atacacgaaa
     5461 ccatcagcgg tgatctcatc taatacttta attaaccgct gccccattcg ggtaaaaaat
     5521 ttgctgtttt caatcgattt gcgtccacct tcggtttcac caatatcggg gtaggtaaaa
     5581 atcaggtcaa tatcggaaga aaaattaagc tctcgtccgc ccaatttgcc catacctaaa
     5641 atcagcagtt cttgtgtttc gccaagagtg ttctttggcg tgccatattc ggcacagcaa
     5701 cgctgaaaca accaatctcg agcggctaga atcaggctct cggctaaatc cgacaaatgc
     5761 tgaaatacca attctaccgt cgctaattta tttgactgaa taaagctcag acgagccaat
     5821 tcacggtggc gaaattgccg caaaaccctc cccaacattt cttcattttc cactcttgct
     5881 aatatttttg ctaaacgctc agtataattg cagcaatcat caggcgtagg aaacttggca
     5941 agccattctg ccagtaaatc cggctgtttt tgcaaggttt gatgcacgaa attggaaagc
     6001 gaaatcgccg tcacaagcgg ttgaatttgc tcagaatttt gcaaattggg cggtaaattg
     6061 agttgggata agggaattag ggtggtaagc ataaatttag gctcaaaatt gaaaatagat
     6121 agaattttac gctcgatcaa ttcccctctt tagcaaagag ggcttagggg agatttggca
     6181 gaggtaattt tactctcata cgaaacatta atatcgtaat caaatctccc ccagcccctc
     6241 ttttctaaag aggggagaaa aaattaaaac actcttttaa acggtttaat aatcacatcg
     6301 ctatacacac cggcttccac ataaggatct tggcttgccc attgttgagc ttcttcaagt
     6361 gagttgaact ctgcaattac agttgaaccg gttacgcttg agccgtcttc agtcgggtta
     6421 ggccctgcga ttaataatcg accttctgcc tgtaattttt ccaaacgagc caaatgagcc
     6481 gggcgaactt ctaaacgttt ttccaacata ttgggattat cttgggcaaa gataacataa
     6541 tacatactat tctccttgtt tttcaggggt taaatcaatt agcgataatg cttcgtctaa
     6601 ttcggggtta ttttctctcg gcacagcacg cggtttgcct tctttatcaa tcgctacata
     6661 ggtaaataag gcttcggtta cgagataacg ttcgcgattg ccctcataaa cttgtttgat
     6721 aaagacctcg actttcactt gcatcgaaga acgccctatg tgcattaact tgccgtagca
     6781 gcagaccaca tcaccgactg aaatcgggcg gtggaaaatc attttatcta ccgttaccgt
     6841 aacgactcta ccttttgcca actctttggc taaaattgca ccgcctaaat ccatctgcga
     6901 cataatccaa ccgccgaaaa tatcgccatt ggcgttagta tctgacggca ttgccaaagt
     6961 tcttaaaatc agtttccctt ccggggcttt tccatagggt ttatctttca tttttttgtt
     7021 cctgcttttc agcatctttt aagtaagggt aaaggacgaa accggtaata accgttgcga
     7081 ctaaggttaa gccaataatg ccaaaggatt tgaaatcaac ccaaatctct tccgataaat
     7141 attgactgat ataaatattc agcagcatac aaatcagaaa gaatactgcc caaccgaagt
     7201 ttattttatt ccacactgct tcaggcaact gaatttcttt gcctaataat tttttgatta
     7261 atggtgtttt gaacacaaat tggctcacta atagcaccaa tgcaaacaga gcgtaaacaa
     7321 tggttacttt ccattgtaaa tatttaactt cattaaaata ggcactcagt aagccgaaaa
     7381 agaccaccga gccacccata ataatttgct gtttttcaat tttgccgtag agcattttta
     7441 aggcgatcat ttgcacgatt gtagcgataa ccaatgcgat agacgccatc tgaataccgc
     7501 tcattttata cacgacaaaa aacagaatca gcggaataaa ttcaagtaac tgtttcattg
     7561 atactcctaa cgcactgccg gcatttggcg gtaaacttgg taaaagcgga agctgaaaat
     7621 cgccataaag acgttaatta ccgcacttaa aacaatggta atgaatatgc caataaagtc
     7681 attaccaaac actgtgaaca aacgagataa catccccggt aataaataag aaatcacaca
     7741 aaataaaatc agcggtagca tttttccacg tgataattga agcgtaaatt taatactttc
     7801 cgatagactt ttctgcggtt tctccaataa ataagcataa atcactaacg ataatttgat
     7861 aaaaaaataa aaaccgacta ccaacaccgg caatgcaata atcactgttt caggcgatgc
     7921 cattgcaaaa gatgcaccga gagaaagcgg taacaccatc acaaattgta acaacagcac
     7981 aggtaggaag tgctttaatg cgtttcctgc attttggaaa aattgacgat aattgccatc
     8041 gttaatcgat tggatattga aaatcattaa gacattgatc aaaagattga caacacctaa
     8101 taaaatagtt ggcaatagta tagacaccat tttagaggca tcaatttgaa gttgttgcgg
     8161 ttgcgactcc gccattagct caggcgaatt tggcgacact aaaataaaaa ccaactgcac
     8221 aaccacaatg gtgacaatgg caaacagtga aaatgcttgt tgattacgca taaagttcca
     8281 actttgctgc aataaatcga caaatttaat tgacataaaa tacccttcaa tagattgtaa
     8341 atattttgtt gtgaagtata gcaaggcttg agccaaactg cattaaccta ttgtagattt
     8401 taatcagtta gctaaaataa aagaaaaatt tgaaagagat aatcctatgt taagcttatt
     8461 tgatgcagga cagcgttacc tcaacactct gccgaatcag aaaaaattag ccaattttat
     8521 gccagattac cacattattc gcttggtaaa attttcctct aaagtaatgc ctgcttttgc
     8581 ttgctttgca ttagtttggc aatattgctt taccgatccg acccaatcta ttttagccaa
     8641 ttcggtactt actgctgttt tcgccatttc tattccgttt caagggttat attggcttgg
     8701 aaaacgggca agatcaccgt tatctttatc attattggaa tggtatgagt cgcttagaca
     8761 aaaattaatt tcggcacaag tcaaattaga agaaacacaa gcggtacctt ctttccaaga
     8821 ttttgcaaat ttactcaagc tagcagaaga gacttgggga gatgaatatt ttgatgagtt
     8881 ataaaataaa aaaagcccca cttaaaataa gtggggtaaa acttttgttc taattctgga
     8941 ggtttgaatt actgttttat tgtggggtac tgatcttttt tgcatagaga gaaagaaaaa
     9001 aatttgggcg gaaaaataac ggcgagcccc gtgaatacaa gggttcgccg ttattgtttg
     9061 gttggagcta tgcaaaaaac aaaatgagca ggattagaaa aaaacgagca gtttttgcgt
     9121 tattttttgc ataggtttga tcggttttaa aactatttta aatctgtttt aaactaaaga
     9181 atgcccgatg tgtaccactt gcccgatgat ttgtaggttt tgggcttggt ctgctgtaat
     9241 ctttattaac tcgtactctt ctcggttgtc tgaaataagc tctacaccgt cccatagcag
     9301 ttttacacgc ttaacccaaa cactgtcgcc gttttgtaca atgtaaatat tattgtcgcc
     9361 tcgtatctca ttacgactta aatcaactag tagctggtcg ccattatcaa tagtcggata
     9421 catcgagtct ccacttgccc aaaaaacagc acacttcttg gcattaacac caagcgtatg
     9481 caacaagcta tcagcataag gctcttgtcc atcaggtttt gttacacctt cattaaagct
     9541 cccaaagcct gcagatactt taatagatgc aaaactatca atcattgaga ttatgttatt
     9601 caaaacggtg cctgataatt cagatccttt gttttgctct tcaggtgtaa tctctcttac
     9661 tatgctatcc ttgattgcat ttattccact tttaaatccg agtgcttctt gggctttacg
     9721 aggcaatgaa gttatgtgat attcattccc cccacctttt actccaactg catcacgttt
     9781 tatccagagt tcttttaatg cctttcttgt aatgtttgtt gcttgctttg gaaggtcagg
     9841 caaatccaat tcttctagtt ctttggctga aaaccaaact ttatca
//
LOCUS       DANLXS010000086         9877 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.086, whole genome shotgun sequence.
ACCESSION   DANLXS010000086 DANLXS010000000
VERSION     DANLXS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.086"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            183..1889
                     /locus_tag="PXD71_001552"
     CDS             183..1889
                     /locus_tag="PXD71_001552"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDL6291660.1"
                     /translation="MNTSSFNRTWAKVILSALMRYGVKHFCIAPGSRSTPLTLAAVQL
                     QQTQYAECHTHFDERGLGFYALGIAKATDSPVAIIVTSGTAVANLYPAVIEASLSHQK
                     LIILSADRPTELIGCGVNQAIHQQGIFANYPVASVNLPKANEAYHASWLVSTIEQACT
                     TQAQQGGVLHINVPFEEPLYEADINEILNTPWLRPIQGWLNNAQTKWIDQQAIQSEVS
                     MHENWDYWRTKRGVIVVGKLPPEQGTGLKTWAETMGWCLLSDVQSCIEASLPYADIWL
                     SNETVKQRLLQADIVIQFGTQIVSKRVNEFLKAFQGEYWVVEQSHDYHNPNAHHQTRF
                     IAKAHHFLRVHPPLRQKPWLLEPLALSQFCSTFIEQQVGGSLSEASLAHNIQDVLAIN
                     GNLFIGNSLFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGIAKGSDRPTVGVVG
                     DISALHDLNSIALLTKINQPTILFVVNNSGGALFDMLPVDSKAKDQYYRLAHNFEFAQ
                     VATMFGLEYIRPFTWADLKTKLKHAYARKGVTIVEIKVNEHDGSNMYKSLLKQISQAE
                     IA"
     gene            1938..2777
                     /gene="menH"
                     /locus_tag="PXD71_001553"
     CDS             1938..2777
                     /gene="menH"
                     /locus_tag="PXD71_001553"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDL6291661.1"
                     /translation="MLSYQWHAQQGTPVVFLHGLLGSQQDWQAVLQHLQNFPQIRPLT
                     IDLPFHGNSKHITCADFANLRQQLHATLESLIGSRPFYLVGYSLGGRAALDYVLNMEN
                     PNLQGVVLEGANIGLSTENDRQIRRQNDRHWANRFRQEPIEQVLEDWYKQPVFADLSD
                     TERECYIDSRKHNQGEQIAQMLEATSLAKQPDYTEKLKQTDKNIVFFIGEKDQKFRKM
                     ASSHQLPTQLIANAGHNAHRANAKNFVEKLGLLILHSDSDLRSQSVKHNGAALLHKSP
                     SGA"
     gene            complement(2826..3503)
                     /locus_tag="PXD71_001554"
                     /pseudo
     CDS             complement(2826..3503)
                     /locus_tag="PXD71_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3589..4251
                     /locus_tag="PXD71_001555"
     CDS             3589..4251
                     /locus_tag="PXD71_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HDL6291662.1"
                     /translation="MRKTHSFQQVRKIKPTWMSVSGHIPFRNGVSIPYESTLERDFLM
                     YFTYLPSVDEVVSQPTTLPFVKNGITYTYTPDFFLSFTDGRKPMLIEVKPKAKWQKHW
                     KEWKEKWKAAIRFCQENGYVFHVYDEDRIRHLALFNLNYVQRYKRIQHEQEDIDAILA
                     QVKLMGNTTIDYLLSRFFAGSLYRMKGLQIIYHLLATKQLHCNWFLPLNEFTEVWGNN
                     DE"
     gene            4241..6169
                     /locus_tag="PXD71_001556"
     CDS             4241..6169
                     /locus_tag="PXD71_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL6291663.1"
                     /translation="MMSNSGFTEKRYHHRLDRGRIILQKGNIYLNREDGEQYELVDYM
                     DEPSQLLVRNLNTRTTKVVSIHQLENFKMNERTDLSVDVTAISNEYWEKAQQKYEAIK
                     PLLGMDQHRPYAVKARAEDVGVNPRTLYRWLQAYNSIGSIAGLVDQKRGWQQGNSRLT
                     PEQDKLIVQVINEFYLHKQRPTTEQTIREIRRRCKIEKVESPSKETIRMRILHISEEE
                     RLRKRGQREKARNKFKPKPNSFPDADYPLSVVQIDHTPVDLIIVDSKYRKPIGRPFLT
                     VAIDIYSRMIVGYYLSLDAPSVTSVAMCIARGILPKERLLLDLGLQGSEWNAFGYPVK
                     VHVDNGPDFQALDLSKSCSAHGIHLEFRPMGRPEYGGHIERVIGTFMKEVHSLAGTTF
                     SNIKERDSYDSEKEAIMTLDEFEKWLVHYIVNVYHKRVHSALGISPEQKWKIGIFGDE
                     NEVGCGYPQLPVDEQTLLLDFLPSITRTIQHNGVTIDGLRYYDVALNMYISDSDESGK
                     SKEFLFRRDPRNISKIWFYDPKLKRYFPIPFANQAMPEMSIWEYREVRSRIANKGDKY
                     INEQQVLDGLTEMREMVAESAQRTKKARRQAERQKMHKASKPIIETKVETKAVVPVVV
                     TSNLLALDDESLSFGEVD"
     gene            6169..7056
                     /locus_tag="PXD71_001557"
     CDS             6169..7056
                     /locus_tag="PXD71_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDL6291664.1"
                     /translation="MQYEHVHEKFRHLVTADNQERIAFLDEPRWLGYGVAKDIMDNLV
                     SLMNKPKRPRMLNLLIVGDSNNGKTTLIRRFFDLYGQAYIDSDSNAIYPILLAEAPPS
                     ANEKELYISLLERFYVPYKPTDTIAKLRYQTIHLFREFRVKMLVIDEFHSLLVGTPRL
                     QRQVMNAIKMLCNELQIPIVGVGTRDAIRVLHTDRQHASRFDVAELPIWKLDKEFQKL
                     LFQFQGILPLRESSNLQSPELATKIHTISGGNLGNVHRLLTACAVQAITSGTEKITLD
                     IIEKNSWVQPTNGYRKIIG"
     gene            7014..8120
                     /locus_tag="PXD71_001558"
     CDS             7014..8120
                     /locus_tag="PXD71_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDL6291665.1"
                     /translation="MGTTNKRLSQNNRIKKGVIHTPIYSNESISSWLIRASFDCGTDP
                     LTFTGFYWEKHRLWTLDLDRGLENIEPTIYDDVQALSGKIQLSEYSLFRRLYPINADE
                     TLAKGQVRWVIPRSARNRTHHIGQQYCPLCLDESPHLRNDWRLAWNFGCLKHNMMLAN
                     RCFCCGTLYQPHLLSAEKRHLNHCHQCGGKLEQGTMEMSLAEKLTLQGINQVLEKDSG
                     ICFGYKVSGKVWFDMLRYCINLLRRASDRPNHALAKFVVNLQIDVENICSPKTGLSFE
                     QLPLGERTNLLVNAYHILGMEADSIRSAIYLCGGTQKTFMFEKYPDFWQEFMALAPAG
                     PNRTRKRTKPQNESITAINRRWERLKRKLHLNNE"
     gene            8117..9541
                     /locus_tag="PXD71_001559"
     CDS             8117..9541
                     /locus_tag="PXD71_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291666.1"
                     /translation="MKINVIKPQEEPCSACGKISRIWRRYNGEGYCANCYAKWFPKKE
                     CSCCHQPKRIYLSSTVCWDCEKQTDCVRCGKAAGTFKIGKITRYGAVCNVCSRYFREE
                     RECFECGQLSRTMLRASESGHSELLCLKCFQKYRFATCKNCRRYRKVHDRDNQLCRKC
                     SEQRTSQCPKCKQDMPAGYGNICADCSRRTLLFNLIRMNVHLFRHKKVKRAYKKFIFW
                     YLAKCGVSVVLKKSDDHVLFFMRCDENWGNIPDYRELVTFFKPNGLRTHLTVLRWLLD
                     TNQVKVDESLKEEMAGLERIQSLFNKLSNSVPCIANYYTQLQHKYEAGKTSLKSVRLA
                     LQPAIDFISSESVSLLPTQVQLNSYLVAKTGQIAAITGFINHLNREYHCELEIDRSLI
                     QKMKEKQLKQQAKQRLAILYKQEKLSIKEQNELLSVVLLNLHNIWIKSPKKLSISEID
                     GIAYYVDNEKKYFIPKDIHIRLME"
     gene            9656..9862
                     /locus_tag="PXD71_001560"
     CDS             9656..9862
                     /locus_tag="PXD71_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA delta(2)-isopentenylpyrophosphate
                     transferase"
                     /protein_id="HDL6291667.1"
                     /translation="MTLKAQFREWLKKEQEHDEMIGFAHIPCILSKVDSKLAIVSGNA
                     YEKKYLRYWIKCFRFDRNISKYIK"
BASE COUNT         3054 a         1991 c         2128 g         2704 t
ORIGIN      
        1 ttcttcgtaa atgctcttaa aaaagagaga gtttactaag taaagtgttg ctcaatagat
       61 agaaattttc acgaagtttt acaaaaacat tccaattttc tcctaaaaaa cttcccacaa
      121 taatttcact ttttgctaca atcctaacca tttatcgaat ttatcaatca gtagagaaga
      181 atatgaatac cagttctttt aatcgcactt gggcaaaagt gattttaagt gcattaatgc
      241 gttatggcgt aaaacatttt tgtattgctc cgggttctcg ctcaacaccg ctcacattag
      301 cggcagtgca acttcaacaa acgcaatatg cagagtgcca cactcatttt gatgagcgtg
      361 gtttaggttt ttatgcgtta gggattgcta aagcaaccga cagcccggtt gctattattg
      421 tcacttccgg cacagcagta gcgaaccttt atcctgcggt gatagaggca agcctgagcc
      481 accaaaaact gattattctt tctgccgacc gcccaaccga attaatcggc tgtggggtaa
      541 accaagccat tcatcagcaa ggtatttttg ccaattaccc ggttgccagt gtcaatctac
      601 cgaaagcgaa cgaggcttac cacgcaagct ggctggtttc gaccattgag caagcctgca
      661 ctacccaagc acaacaaggc ggtgtgttgc atatcaatgt gccttttgaa gagccgcttt
      721 acgaagcaga cattaatgaa attctaaata caccttggtt acgcccaatc caaggctggt
      781 tgaataacgc tcaaaccaaa tggatagacc aacaagcgat tcaaagcgaa gtctcaatgc
      841 acgaaaattg ggattactgg cgaactaaac gaggggtgat tgtggtcggc aaactgccgc
      901 cggaacaagg cacaggttta aaaacttggg cggaaaccat gggttggtgc ttgctaagtg
      961 atgtgcaatc ttgcattgaa gccagcctgc cgtatgccga tatttggctc tccaacgaga
     1021 ctgtcaaaca gcgtttattg caagcggata tcgtgattca attcggcacg caaattgtca
     1081 gcaaacgggt gaatgaattt ttaaaagcat tccaagggga atattgggtg gtggaacaat
     1141 cgcacgatta ccataatcca aacgctcatc atcaaacccg ttttattgcc aaagcacatc
     1201 actttttgcg ggtacaccca ccacttcgcc aaaagccatg gttgcttgag ccattggcat
     1261 tatcgcaatt ctgctccact ttcatagagc aacaagtggg tggcagctta agtgaagcct
     1321 cacttgcaca caacattcaa gatgttttag caattaatgg caacttattt atcggcaata
     1381 gcttatttgt tcgattggtt gatgctctgt gcaaactgcc ggaaggctac cctgtttaca
     1441 ccaaccgtgg tgcaagcggt attgatggct taatcgcaac aatggcaggc attgcaaaag
     1501 gtagcgaccg cccgactgtc ggtgtggtag gtgatatttc cgcattacac gatcttaact
     1561 caattgcatt gctcaccaaa attaaccagc cgaccatttt atttgtggtg aataacagcg
     1621 gtggtgcgtt gtttgatatg ttgcctgttg atagcaaagc gaaagatcaa tattaccgcc
     1681 ttgctcacaa ctttgaattt gctcaagtcg ccacaatgtt cgggcttgaa tatatccgcc
     1741 cgtttacttg ggcagatcta aaaaccaaac tcaaacacgc ctatgctcgt aaaggtgtta
     1801 ccattgtgga aatcaaagta aatgaacacg acggcagcaa tatgtataaa tcattgctaa
     1861 aacaaatttc acaggcggaa attgcctaat cacaactgcc cctcgccaat cctgcgaggg
     1921 gtaaaaatgt atcgactatg ctttcctatc aatggcacgc tcaacaaggc acacccgtgg
     1981 ttttccttca cggcttgctc ggctcacaac aagactggca agctgttctc caacatttgc
     2041 aaaattttcc gcaaattcga ccgcttacca ttgaccttcc ttttcacgga aatagtaagc
     2101 acattacctg tgccgatttt gccaacctgc gacaacaact acacgccacc cttgaaagcc
     2161 taatcggctc acgacctttc tatttagtcg gctactcact cggtggacga gcggcactcg
     2221 attatgtgtt aaatatggag aatccgaatc tgcagggcgt ggtgttagaa ggggcgaata
     2281 tcggcttaag cacagaaaac gaccgccaga ttcgccggca aaatgaccgc cattgggcaa
     2341 accgtttccg tcaagaaccc atcgaacaag tgcttgaaga ttggtacaaa cagcctgttt
     2401 ttgcggattt aagcgatacg gaaagagaat gttatattga tagcagaaag cacaatcaag
     2461 gagagcaaat tgctcaaatg ctggaagcca ccagcctcgc caaacagcct gactacaccg
     2521 aaaaactcaa gcagacggat aagaatattg tcttttttat cggtgaaaag gatcagaaat
     2581 ttcgcaaaat ggcatcaagc caccaactac caacacaact gattgctaat gcagggcata
     2641 acgctcatcg ggctaatgct aaaaattttg tggagaaatt ggggttattg atactccact
     2701 ccgacagcga cctacggtcg caatcggtta agcataacgg tgcagctctg ctgcacaaaa
     2761 gccccagtgg ggcttaatca gccgtaggct aaatataacc tagtacggct cagccgtgct
     2821 aggtgttagg gctccccctg caaccgttgc aataagcgat ccatcgcccg ataaccaagt
     2881 gcttccgcta aatgcagttg ttgaatattc gcctcgccgg ctaaatcagc aatagtccga
     2941 gaaactttca aaatacggtg gtaggctcgt accgataaac cgagctttgt caatgcagtt
     3001 tctaaaaata gggcatcttg tgccgataat tggcaatctc gctcaatttc tcgggtggtg
     3061 agttgagaat tgattttacc tgctcgctca aattgcattt ttctggctaa aaacactcgc
     3121 ttgcgaacct gctcggtggt ctctcctcga ttctccgtcc cactttgcaa tgctccttgt
     3181 ggcaaaagtg gcacttctat agagagatcg aaacggtcta agaaaggtcc ggaaaggcgg
     3241 tttaaatacc gcattacctg ttggggcgaa gtgcggttat gcgtgccttg ataatgccct
     3301 gtcgggcttg ggttcatcgc tgcaattaat tgaaagcgtg ccggaaattg tactttagcg
     3361 ttggctcggg aaatcacaat ttcgcctgcc tccaacggct gacgtaacgc atcaagcact
     3421 ttacgttcaa attcaggcaa ttcatctaag aacagcacac cgttatgggc aagtgaaatt
     3481 tcccccggtt tggggatcga gccactgtaa aaactagaaa taaatgtcat aaaaactaga
     3541 aatagctgtc agattattta gaaaacaaga aataggtgtc aaattattat gagaaaaaca
     3601 catagttttc aacaggttag aaaaattaaa cccacttgga tgagtgtatc ggggcatatt
     3661 ccatttagaa acggagtcag tattccctat gaatcgactt tagagcgtga ttttttgatg
     3721 tatttcactt atctaccttc agtagatgaa gtcgtctctc aaccgactac cctacctttc
     3781 gtcaagaatg ggatcactta tacctataca ccagatttct tcttgtcatt tacagacggt
     3841 cgaaagccga tgttaatcga ggtcaaaccg aaagcaaaat ggcagaagca ctggaaagaa
     3901 tggaaagaaa aatggaaagc tgcaatacgt ttttgccaag aaaacggtta tgtattccat
     3961 gtgtatgatg aagatcggat tcgacatcta gcgcttttca atctcaacta tgttcagcgc
     4021 tataagcgaa tacaacatga acaagaagat atagatgcta ttctagccca agtaaagctg
     4081 atgggaaata ccactattga ctatttactc tctcgatttt ttgcaggttc tctatatcgc
     4141 atgaaaggcc tacaaatcat ctatcactta ctggcaacga aacaactaca ttgcaattgg
     4201 tttctaccac ttaatgaatt taccgaggta tgggggaata atgatgagta actctggatt
     4261 tacagaaaag cgctatcatc accgattaga tcggggacgt attattttgc aaaaaggtaa
     4321 tatttaccta aatagagaag atggagaaca gtatgaattg gttgattata tggatgaacc
     4381 ctcccaactg ctcgtacgaa atctcaacac tcgtacaacc aaagtcgtca gtattcatca
     4441 gcttgaaaat ttcaaaatga acgagcgaac ggatctttct gtggatgtaa ccgctattag
     4501 taatgaatat tgggagaaag cccaacaaaa atatgaggca attaaaccgt tattagggat
     4561 ggatcaacat cggccttacg cagttaaggc tcgtgcagaa gatgttgggg tgaaccctcg
     4621 tacattatac cgttggttac aagcctataa ttccatagga tctatcgcag gtttagtcga
     4681 tcaaaaacgt ggatggcaac aaggaaattc ccgtttaacg ccagagcaag acaagctgat
     4741 cgtgcaggta atcaatgagt tttacctaca taaacaacgc ccaactactg agcaaactat
     4801 tagagaaatt cgccgccgat gcaaaattga aaaagtggaa agcccaagca aagaaaccat
     4861 ccgtatgcgc attttgcata tttcagagga agagcgatta cggaaacgcg ggcaacgaga
     4921 aaaggcacgc aacaaattta agccgaagcc aaatagcttt cctgatgcgg attatccatt
     4981 gagtgtggta caaattgacc atacaccagt tgacctaatt attgtggata gtaagtatcg
     5041 taaaccgatt ggccgaccgt ttttgacggt tgcgatagat atatacagca gaatgattgt
     5101 tggttattac ctttcacttg atgcgccttc tgtaacttcg gtggcgatgt gtattgcacg
     5161 tggtattttg ccgaaagaac gcctactgct tgatttaggt ttgcaaggct cagaatggaa
     5221 tgcttttggc tacccagtga aagtgcatgt ggataatggc ccagattttc aggctttgga
     5281 tttatctaaa tcttgttctg cacacggaat ccatcttgaa tttagaccga tggggcgacc
     5341 tgaatatggc ggacatattg agcgtgttat cggtacattt atgaaagaag ttcacagttt
     5401 agcaggaaca acattctcca acatcaaaga gcgtgactct tacgattcgg aaaaagaggc
     5461 cattatgact ctcgatgagt ttgaaaaatg gttggtacat tacattgtga atgtttacca
     5521 caaacgtgtg cattctgctt taggcatttc tcctgaacaa aaatggaaga ttggtatttt
     5581 tggagatgaa aatgaagtgg gatgtggtta tccgcaactt cctgttgatg aacaaacact
     5641 actacttgat ttcttgccaa gtatcactcg taccattcag cataatggtg taacaattga
     5701 cggacttcgt tattacgatg ttgcgctaaa tatgtatatc agtgattcag atgaaagcgg
     5761 taaatctaag gaatttctgt ttcgccgaga cccgcgaaat atcagcaaaa tttggtttta
     5821 tgatccgaaa ttaaaacgtt acttcccaat tccatttgca aatcaggcaa tgcccgaaat
     5881 gagcatttgg gaatatagag aggttcgtag ccgaattgcg aataaaggtg ataaatatat
     5941 caacgagcaa caagttttag acggtttaac cgagatgcgt gaaatggttg ctgaatcggc
     6001 tcaacggacg aaaaaagcac gccgtcaggc tgaacgacaa aaaatgcaca aagcaagtaa
     6061 gccaattatt gaaacaaaag tggaaactaa ggctgttgta ccagtcgtag taacatcgaa
     6121 tttgcttgct ttagatgatg agagcttatc ttttggtgag gtggactaat gcaatacgaa
     6181 catgttcatg aaaaattccg ccatcttgtg acagcggaca atcaggagag gattgcgttc
     6241 ttggacgagc caagatggtt aggttatggt gtcgccaaag acattatgga taatttggtt
     6301 tctttaatga ataaaccaaa acgcccacga atgttgaatt tgttgattgt cggcgattcc
     6361 aataatggaa aaaccacact gatccgccgc ttcttcgact tgtacggtca ggcgtatatc
     6421 gacagtgatt ctaatgcgat ttaccctatt cttcttgccg aagccccacc aagtgcgaat
     6481 gaaaaagaat tgtacatctc attattagag cggttctacg taccatataa gccgacagat
     6541 acgatagcca aacttcgtta tcagactatc catttgttcc gagaatttcg tgtcaaaatg
     6601 ttggttattg acgagtttca ttctctgttg gttggtacac cacgcttaca acgacaggtg
     6661 atgaacgcaa ttaagatgct ttgtaacgag ctacaaattc caattgtcgg tgttggtact
     6721 cgtgatgcaa ttcgggtatt acatacggat cgacaacatg ccagtcgttt tgatgttgcg
     6781 gaattgccaa tatggaaact agataaggaa tttcaaaaac tcctatttca attccaaggg
     6841 attttacctt tgagggagag ttcaaatctg caatctccag aacttgctac gaagatccac
     6901 accatttcag gtgggaactt agggaacgtg catcgcttac taacagcttg tgcagttcaa
     6961 gccattacaa gtggcacgga aaaaatcacc ttagacatca ttgagaaaaa ttcatgggta
     7021 caaccaacaa acggctatcg caaaataatc ggataaaaaa aggtgttatt cataccccta
     7081 tttattcaaa tgaaagtatt agctcgtggt taattcgtgc atcctttgat tgtggtacag
     7141 atccactgac ttttacaggg ttttattggg aaaaacatcg gttgtggaca ttagatttag
     7201 atcgtggctt ggaaaatatt gagccaacta tctatgatga tgtacaggca ttatcgggta
     7261 aaatccaact ttcagaatat tccttgtttc gcaggcttta ccctataaat gcagatgaaa
     7321 ccttagctaa aggtcaagta agatgggtga ttccacgttc ggcaaggaat agaacccatc
     7381 acattggtca acaatattgt cctttgtgct tggatgagtc gccacattta cgcaatgatt
     7441 ggcggttagc gtggaatttt ggttgtctca agcataatat gatgttggcg aacagatgtt
     7501 tttgttgtgg tacattgtat caaccccatt tactatcagc ggaaaagagg catttgaatc
     7561 attgtcatca atgtggtggg aaactggaac aaggcacaat ggaaatgagt ctagctgaaa
     7621 agctgacatt gcaaggtatt aatcaggtac tcgaaaaaga ttcaggcatc tgttttggat
     7681 acaaggtgtc gggtaaggtg tggtttgata tgctccgcta ctgcatcaac ttattgagac
     7741 gtgcatcaga cagaccaaat catgccttag caaaatttgt agttaatcta cagattgatg
     7801 ttgaaaatat atgtagcccc aaaacaggac tttcttttga acaacttccg ctaggtgaac
     7861 gtacaaattt acttgtgaat gcctatcata ttttgggaat ggaagcagat tctattcgta
     7921 gtgcaattta tttgtgtggc ggaacacaaa aaacctttat gtttgaaaaa tatcctgatt
     7981 tttggcaaga atttatggca ctagcacccg caggcccaaa tagaacacga aaacgcacca
     8041 agccccaaaa tgagtctatc acagcaatta atcgccgttg ggagcgtttg aaacgcaaac
     8101 tacacttaaa taacgaatga aaattaatgt aataaaacca caggaagaac cttgctccgc
     8161 ttgtggcaaa atttctcgca tttggcgcag atataatggc gaaggctact gtgccaattg
     8221 ctatgctaag tggtttccga aaaaagagtg cagctgttgc catcaaccaa agcgtatcta
     8281 tttgagttca acagtatgtt gggattgcga aaaacagact gactgtgtac gttgcggtaa
     8341 ggctgcgggt acattcaaaa ttggtaaaat tacccgttat ggtgcagttt gtaatgtgtg
     8401 ttcacgttat tttcgtgaag aacgagaatg ttttgagtgt ggtcaactat ctagaactat
     8461 gcttcgagca tcagagtcag gtcatagcga gctactctgc ttgaagtgtt ttcaaaaata
     8521 tcggtttgcg acttgtaaaa actgtcgccg ttatcgcaaa gttcatgacc gtgataatca
     8581 gctttgtcga aaatgtagtg agcaacgaac ttcacaatgc ccgaagtgca aacaggatat
     8641 gcctgcggga tatggtaata tttgtgcaga ttgttctagg cgaactctac tgttcaacct
     8701 tattcgaatg aatgtgcatt tattccgcca caaaaaagtg aaaagagcat ataaaaagtt
     8761 cattttttgg tatctcgcaa agtgtggcgt gagtgttgtg ctgaaaaaat cagacgacca
     8821 cgtcctcttt tttatgcgtt gcgatgaaaa ttggggcaac attccagact accgtgagtt
     8881 ggttacattc tttaaaccca atggattacg gacgcatttg actgtgttgc gttggttgct
     8941 tgataccaat caagtcaaag tagatgagag cttaaaagag gaaatggcgg ggttggaacg
     9001 tattcaatcc ctatttaaca aattaagtaa ctctgtacct tgtattgcaa actactacac
     9061 gcagttacaa cataaatatg aggcgggcaa aacatcactg aaatctgttc gattagcttt
     9121 acaacctgcg attgatttca tcagttcaga atctgtctcg ttactgccga ctcaagtaca
     9181 actgaatagc tatcttgtgg caaaaacggg acagattgct gctattacag gctttataaa
     9241 ccacctgaac cgagaatatc attgtgaatt agaaattgac cgctccctca ttcaaaagat
     9301 gaaagaaaag cagttgaagc agcaagcaaa acagcgatta gcgatactct acaagcaaga
     9361 aaaactaagt atcaaagagc aaaatgagct actctcagtc gtattactga atttgcacaa
     9421 catttggata aaatcaccga aaaaattaag tatttcagag atagacggta tagcctacta
     9481 tgttgataat gagaaaaaat actttatacc aaaagacatt cacatacgcc tgatggaata
     9541 agctgtcgtc aatttttatt tttgcgatct gtgtcgagaa aataatattt atgaatcgac
     9601 agatgaaata atattgataa agtctctttg tgtgttcaac aaacaggaga taaatatgac
     9661 attaaaggct caatttcgtg aatggcttaa aaaagagcaa gaacatgatg aaatgatagg
     9721 gttcgcccat atcccttgca ttttatctaa agtggatagc aaactggcga ttgttagcgg
     9781 aaatgcttat gagaagaaat atctcagata ttggatcaag tgttttaggt tcgatagaaa
     9841 tatcagcaag tatattaaat aggttaaata tctagct
//
LOCUS       DANLXS010000087         9795 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.087, whole genome shotgun sequence.
ACCESSION   DANLXS010000087 DANLXS010000000
VERSION     DANLXS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.087"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            124..804
                     /locus_tag="PXD71_001561"
     CDS             124..804
                     /locus_tag="PXD71_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HDL6291668.1"
                     /translation="MALNNILGLFAQSPLKPLQKHSNKVTECSELLESFFEATFEHDW
                     EKAAEVRGKIVDLERRADALKREIRLKLPRGLFMPVERGDLLELVTQLDKLANYSRDI
                     SGRIIGRKLIIPTEMQPLFKKFLSRSIDASRQVRKVLDEMDQLLETGFRGRELDFVNK
                     MILELDQIEDDTDQYQITLRHTLLELEKNLNPIDVMFLYKCIERISVLADQAQRIGSR
                     IELMLAKS"
     gene            816..2084
                     /locus_tag="PXD71_001562"
     CDS             816..2084
                     /locus_tag="PXD71_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDL6291669.1"
                     /translation="MELLNQYGSILVIITAIVGFMMAFGIGANDVSNAMGTSVGSGTI
                     TIKQAILIAMVFEFAGAYLAGGEVADTIKSGIIDPAQFANSPDILVLGMMASLCAAGM
                     WLIIATKMGWPVSATHTIIGAVIGFALITIGSDAIQWDALGGIVGSWFVTPILAGIVA
                     YSIFINSQKLIFNRSNPFKQAKKYGPMYMGITIFILVIVTVSKGLKHVGLHLDTAETL
                     GISFALALLAFIIGYFYFRSKSFAMKARAEEKGFAGVEKIFSILMLITACAMAFAHGS
                     NDVANAIGPLAAVESIVRSGGMIEGKTTLAPWILPLGALGMMIGLAIMGKNVMATVGT
                     GITDLTPSRGFAAQFACAVTVVIASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVI
                     RNIVISWVITLPVGAVLSIIIYYILSAIFY"
     gene            2139..2750
                     /locus_tag="PXD71_001563"
     CDS             2139..2750
                     /locus_tag="PXD71_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDL6291670.1"
                     /translation="MKKLSSALLSCFLLGLSSPALTADTYITENLSTFMRKGAGDQFR
                     ISGAIQAGEKVTVLDRKERYSLIRDSRNREGWVLNSDLSDTASPKELIPQLKQQVQDL
                     TNRLSKIDTEWQQRTAEMQRRTQDSVQKSSDLVEENAQLKRELEILKNKNRDLETMHD
                     SEKREIVIQWFIYGGAVLGAGLLLGLLVPFLIPRRRRNNSGWA"
     gene            complement(2804..4102)
                     /gene="aroA"
                     /locus_tag="PXD71_001564"
     CDS             complement(2804..4102)
                     /gene="aroA"
                     /locus_tag="PXD71_001564"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL6291671.1"
                     /translation="MEKLTLTPISRVEGEINLPGSKSLSNRALLLAALATGTTQVTNL
                     LDSDDIRHMLNALKALGVKYELSDDKTVCVVEGIGGAFKVQNGLSLFLGNAGTAMRPL
                     AAALCLKGEEKSQIILTGEPRMKERPIKHLVDALRQVGAEVQYLENEGYPPLAISNSG
                     LQGGKVQIDGSISSQFLTALLMSAPLAEGDMEIEIIGDLVSKPYIDITLSMMNDFGIT
                     VENRDYKTFLVKGKQGYVAPQGNYLVEGDASSASYFLASGAIKGKVKVTGIGKKSIQG
                     DRLFADVLEKMGAKITWGEDFIQAEQSPLKGVDMDMNHIPDAAMTIATTALFAEGETV
                     IRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLALENFQHAEIETYNDHR
                     MAMCFSLIALSNTEVTILDPNCTAKTFPTYFRELEKLSVR"
     gene            complement(4148..5242)
                     /locus_tag="PXD71_001565"
     CDS             complement(4148..5242)
                     /locus_tag="PXD71_001565"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL6291672.1"
                     /translation="MQFINIANEGVKSLSPYQAGKPIEELERELGIQNIIKLASNENP
                     FGFPESAKKAILNQLDDLTRYPDSNGFELKTTIAQKFGVQANQITLGNGSNDLLELFA
                     HTFAGEQDEIIYSQYAFIVYPLVTKAINAVAREILAKNWGHNLDAFLAAITEKTKLIF
                     IANPNNPTGNFLTQAEIEGFLAKVPANVIVVLDEAYTEFTAPQERVDSFGLLAKYPNL
                     IVSRSLSKAYGLAGLRIGYAVSNPEIADLLNRVRQPFNCNSLALASAIAVMNDDEFVK
                     KVAENNRLEMARYEAFCQANGLEYIPSKGNFITIDFKRPAAPIYEALLREGVIVRSIA
                     GYGMPNHLRISIGLPQENERFFTALLKVLA"
     gene            5514..6833
                     /locus_tag="PXD71_001566"
     CDS             5514..6833
                     /locus_tag="PXD71_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL6291673.1"
                     /translation="MNHLLYSVEDKPPFGLSLLLASQHLLAALGGIIAVPLVLGNVLK
                     LSTADTIILVNAALLISGIVTIIQCRGIGPIGIRLPSVMGTSFTFVAAALAIGFSESG
                     IAGIMGASLVGSLVMIIGSFFMPYIRKLFPPVVTGTVIMMIGLSLIPVAVDWFAGGQR
                     TDPHYATPENLIMASFVLIIVVALVQWGKGIFSAAAIVIGMMIGYIVSLALGWVSFNG
                     VKEAQLFALPQPLHFGLEFPISGIIGMSIAYLVTIVESSGNFLALGNATKTEITGKHL
                     RGGVLCDGLGSAFAAIMSTTPFSSFSQNIGVISLTGVASRYVVAFTGALLVLAGLFPV
                     FGALIVSIPLPVLGGAGLMMFAMIIAAGIQMLDNIQRSKRNGLIIAISIGCGLAVTTR
                     PELLDKLPHFFKEVFGSGITVGSLLALILNLVLPEDKITKKENKSDE"
     gene            complement(7003..8946)
                     /gene="ftsH"
                     /locus_tag="PXD71_001567"
     CDS             complement(7003..8946)
                     /gene="ftsH"
                     /locus_tag="PXD71_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL6291674.1"
                     /translation="MVKNILLWVVIAIVMMTAYEGFNSNFSGSSNTVPYSTFLSDIKE
                     NRLKGVDFKQNDDTILVTKNDGATYQTVMPMYDEYLMADLAKTNAVITGQPAERRGLL
                     SQILISWFPMLLLIGFYVFYMRQMQGGGGRGAMGFGKNKAKMLTADEVKTRFSDVAGC
                     DEAKEEVGEVVEFLRDPTKFQKLGGRIPKGILMVGPPGTGKTLLAKAIAGEARVPFFT
                     MAGSDFVEMFVGVGASRVRDLFDQAKKNAPCIIFIDEIDAVGRKRGGAGFSGGHDERE
                     QTLNQMLVEMDGFEGSEGVIIIAATNRADVLDDALTRPGRFDRQVTVDLPNVKGREQI
                     LKVHLKKVPLAEGVDPMQIARGTPGYSGAQLANLVNEAALFAARKNKRVVMMEDFEEA
                     RDKINMGPERRSNTMTEKEIINTAYHEAGHVIVGYLMPEHDPLNKVTIVPRGQALGFA
                     QFLPEGDRVSETFTKLESQLSTLFAGRIAEGLIFGEDKITTGASSDIHRATQIARAMV
                     TQWGFSKELGPIFYQNEDGMGAIKGVSEESQKLIDVEMRKIIDRNYQRAKQTLEENMD
                     ILHAMKDALLKYETLDSKQIDDLMARRPVGEPSGWTDTPTKDDNATPPSGEQAADVSS
                     TLEDNPNKDNNIGDSLADTKAEH"
     gene            complement(9067..9693)
                     /gene="rlmE"
                     /locus_tag="PXD71_001568"
     CDS             complement(9067..9693)
                     /gene="rlmE"
                     /locus_tag="PXD71_001568"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDL6291675.1"
                     /translation="MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQT
                     DRLFKPGMTVVDLGAAPGGWSQYVVTQIGSKGRVIACDILDMNPIVGVDFLQGDFREE
                     SVLNALLERVGEDKVNVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLATKGSF
                     VVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRDRSREVYIVATGYKG"
BASE COUNT         2750 a         2095 c         1900 g         3050 t
ORIGIN      
        1 aaaacaaacc gcttgtctta aaaacataat ttaatttcgt gatctttact aatacatcaa
       61 gtaaaatacg ccggacttta cagtaagaag tcattgtcaa tattatcact atggagatat
      121 tttatggcat tgaataacat tttaggttta ttcgcccagt ccccacttaa accgttacaa
      181 aaacactcaa ataaagtgac tgaatgtagc gagttattag aatctttttt tgaagcgact
      241 tttgaacatg attgggaaaa agcagctgaa gtgcgcggta aaattgttga tttagaacgc
      301 cgcgcagatg cccttaaacg tgaaatccgc ttaaaactgc ctcgtggttt atttatgcca
      361 gttgaacgcg gtgatttatt agaactagtc acacaattag ataagttagc aaactattca
      421 agagatattt ccggtcgcat tattggtcgt aaattaatca ttcctaccga aatgcaacca
      481 cttttcaaaa aattcctttc tcgtagtatt gatgctagcc gtcaggtacg caaagttttg
      541 gatgaaatgg atcagttatt agaaactggt tttagagggc gagagcttga tttcgtcaat
      601 aaaatgattc ttgaacttga ccaaattgaa gatgataccg accaatatca aattacatta
      661 cgtcatacac ttcttgagtt agaaaaaaac ctgaacccta ttgatgtgat gtttttatac
      721 aaatgtattg aaagaatcag cgttctagct gaccaagccc agcgaattgg ttctcgtatt
      781 gaacttatgc tggcgaagtc ataggagaaa attaaatgga attactcaat caatatggct
      841 caatactggt gattattact gctattgtag ggtttatgat ggctttcggt attggtgcta
      901 atgatgtttc aaatgccatg ggaacatccg tcggctctgg aacaattacg attaaacaag
      961 ctattttaat tgctatggta tttgaatttg ctggtgctta tctggcaggc ggtgaagtcg
     1021 cagatacaat taaaagtggc attattgatc cggcacaatt cgccaatagc cctgatatcc
     1081 tggtacttgg aatgatggca tctctctgtg ctgccggaat gtggctaatt attgcaacta
     1141 aaatgggttg gccggtttca gcaactcaca caatcattgg tgccgttatt ggttttgcat
     1201 taatcactat tggttcagat gcaattcaat gggatgcatt aggtggaatt gttgggagtt
     1261 ggtttgtgac ccctattctt gcggggatcg tagcatattc gatctttatt aactcacaaa
     1321 aactgatctt taaccgctca aacccattca aacaagcgaa aaagtatggc ccgatgtaca
     1381 tgggaattac tatttttatt ttagttattg taaccgtgtc aaaaggctta aagcacgtag
     1441 gattacatct agatacagct gaaacattgg gaatttcatt cgcgcttgcc ttacttgctt
     1501 ttatcattgg atatttttat ttccgcagta aaagctttgc aatgaaagct cgtgcagaag
     1561 agaaaggctt cgctggcgtt gagaaaattt ttagtatttt aatgcttatt acggcctgtg
     1621 ctatggcttt tgctcatggc tctaatgatg ttgctaatgc tattgggcct ctagctgctg
     1681 ttgaatctat tgtaagaagt ggtggaatga ttgaaggtaa aacgacttta gccccttgga
     1741 ttctcccact aggcgcctta ggtatgatga ttggtttagc tattatgggc aagaatgtaa
     1801 tggctacagt aggtactggt attaccgacc ttactccaag ccgtggcttt gctgctcaat
     1861 ttgcctgcgc cgtcacggtt gtcattgcat caggtactgg tttaccaata tcaacaaccc
     1921 aaacgcttgt aggagcagta ttaggtgtgg gattcgcccg tggtattgct gcacttaact
     1981 taggcgttat tcgtaatatt gttatatcat gggtaattac cttgcctgta ggtgctgtgc
     2041 tttcaattat tatctattat attttaagcg ctattttcta ttaatttttc aaaaagaata
     2101 caggcaactg tattcttcct agctttagag gaatatatat gaagaaatta tcatcagctc
     2161 ttctgtcttg ttttttactc ggcctatcta gccctgcttt aacggcagac acttacatta
     2221 ctgaaaatct gagtacattt atgcgcaaag gtgctggtga tcaattccgc atttcaggcg
     2281 ctattcaagc aggtgaaaaa gtcacagtat tagatcgtaa agagcgctac agtctgattc
     2341 gagatagtcg taaccgtgaa ggttgggtac ttaattcaga cttaagcgat actgctagtc
     2401 caaaagagct gattcctcaa ttaaaacaac aggttcaaga tttaaccaat cgcctgagca
     2461 aaatcgatac cgaatggcaa caacgtaccg cagaaatgca acgtagaacg caagattcag
     2521 tgcagaaaag cagtgatttg gttgaggaaa atgcacaact aaaacgtgaa ctggaaatcc
     2581 tgaaaaacaa aaatcgtgat ttagaaacaa tgcacgattc cgagaaacgt gaaatcgtga
     2641 ttcagtggtt catctatggt ggtgctgtgt taggcgcagg tttactactt gggttactcg
     2701 tgcctttcct gatccctcgc cgcagacgta acaacagcgg ttgggcttag tctgccacaa
     2761 taaaacgtaa aaaggattca gttttctgaa tcctttttta cttttatctg accgataatt
     2821 tttccaactc cctaaagtaa gtcgggaacg ttttagcggt acaatttgga tctaagatcg
     2881 tcacttctgt attcgataac gcaattaatg aaaaacacat tgccatacgg tgatcgttat
     2941 aggtttcaat ttcagcgtgc tggaagtttt ctaacgcaag cggttgaatc cgaataaaat
     3001 cttccccttc ttctacctct gccccgactt tacgcaattc ggttgccatt gctgtcaagc
     3061 ggtcggtttc ttttacccgc cagttataaa tattgcggat aactgtttct ccttcggcaa
     3121 ataaagcggt tgttgcaatc gtcattgccg catcaggaat atgattcata tccatatcta
     3181 cgccttttag cggggattgc tcggcttgaa taaaatcctc tccccaagtg atttttgccc
     3241 ccattttttc caacacatcg gcaaacaagc ggtcgccttg gatcgatttt ttaccaatgc
     3301 ccgttacttt taccttgcct ttaatcgcac cggaggctaa gaaataagag gcagaagagg
     3361 catctccctc caccaaataa ttaccttgtg gagcaacata gccttgttta cctttaacta
     3421 aaaaggtttt gtaatctcga ttttcaaccg taataccaaa atcgttcatc atcgaaaggg
     3481 taatatcaat ataaggtttt gataccagat caccgataat ctcaatttcc atatcgcctt
     3541 ccgctaatgg ggcagacatc agcaatgcgg ttagaaattg gctggaaatc gagccgtcaa
     3601 tttgcacttt tccgccctgc aaaccgctat tgctaattgc caacggtgga tagccttcat
     3661 tttctaaata ctgtacctct gcccctactt ggcgtaaagc atcgactaag tgtttaatcg
     3721 ggcgttcttt cattcttggt tcaccggtaa gaatgatttg ggatttttcc tcacctttta
     3781 aacacaatgc tgctgcaagt ggtcgcattg ccgtgcctgc attgccgaga aacagtgata
     3841 agccgttttg aaccttaaaa gctccaccaa tcccttcaac tacacagacg gttttatcgt
     3901 ccgatagctc atatttcacg cctaacgctt ttaaggcatt gagcatatgt cgaatatcat
     3961 cactatctaa taaattggtc acttgagtcg taccggtggc taaggcggct aataataagg
     4021 ctcggttaga caggctttta gaaccaggta aattgatctc gccttctact cgggaaatcg
     4081 gggttaaagt tagtttttcc atttttctct ttaaatttgt aaaattttca taaaaggtaa
     4141 ccgcttgtta agctaacact ttcaataagg cagtaaaaaa tctctcgttt tcttgcggta
     4201 aaccgatact aatgcgtaaa tgattcggca ttccataacc tgctatcgag cgaacaatca
     4261 cgccttcacg cagtaatgcc tcataaatcg gagcggcagg acgtttgaaa tcaatagtaa
     4321 taaaattgcc cttagaaggg atatattcca agccgtttgc ttggcagaaa gcctcatagc
     4381 gagccatttc caagcggtta ttttctgcca cttttttgac aaattcatca tcattcatta
     4441 ccgcaatcgc tgaagctaag gcaagactat tgcagttaaa cggctggcga acacgattta
     4501 ataaatccgc aatttcaggg tttgaaaccg cataaccaat ccgaagcccg gctaagccgt
     4561 aagccttaga gagtgaacgt gacacaatta aattcgggta ttttgccaat aagccaaacg
     4621 aatccacccg ttcttgaggc gcagtaaact cggtataagc ttcgtccagc accacgatta
     4681 catttgccgg cactttagct aaaaagccct caatttccgc ttgggttaaa aaattaccgg
     4741 tcggattatt cgggttggca ataaaaatta atttagtttt ttcggtaatt gcagccaaaa
     4801 aggcatctaa attgtgtccc caatttttag ccagaatttc acgggcaacg gcattaatcg
     4861 ctttcgtcac caacggatac acaataaacg catactgaga atagataatt tcatcttgct
     4921 caccggcgaa ggtgtgggca aacagctcta ataaatcgtt tgagccattg cctaaggtga
     4981 tttgattggc ttgtacgcca aatttttggg caatagtggt ttttaattca aagccgtttg
     5041 aatccggata gcgggttaaa tcgtcaagct gattcagaat cgcctttttt gcactttcag
     5101 gaaaaccaaa tgggttttcg ttcgaggcta atttaatgat attttgaatc cctaattcac
     5161 gctctagctc ctcaatcggc tttcctgctt ggtaaggcga aagggatttc acgccctcat
     5221 ttgcgatgtt gataaattgc ataataacct ctgattattc ttataaaata taagttcgga
     5281 ttgtagtaaa gaatgattgc ctttaccagt aaattttttg ttacaactgg aaagaaattg
     5341 gtacaaacat tcggattagc tttgcaaaaa gcgtaaaaaa tcttaccgct tgtcatttat
     5401 cagttaggga aaataaaaaa tcactctctt atataaaaac aattacgcaa acgattgctt
     5461 attaaaaaaa cttcagtata atgccgacat tttttaactc tcgaggaatc actatgaacc
     5521 atttacttta ttctgtcgaa gacaaaccgc ctttcgggct gagcttattg cttgcctcac
     5581 aacatttatt agccgcttta ggtggcatta tcgctgtacc tttagtgcta gggaatgtac
     5641 tcaaactctc tactgcggat actataatct tagttaatgc tgcactttta atttcaggca
     5701 tcgtcactat tattcaatgt cgaggtattg gcccaatcgg gattagacta ccgagtgtaa
     5761 tgggaactag ctttacgttt gttgcagcag cattagcgat tggtttcagt gaaagtggca
     5821 ttgccggcat tatgggagcc tctttagttg gctctctagt gatgattatc ggcagttttt
     5881 ttatgcctta tatccgtaag ctttttccac ccgtggtaac aggtactgtc ataatgatga
     5941 ttggcctaag tttaattcct gttgcagtag actggtttgc tggtggtcag cgtactgatc
     6001 cacactatgc aaccccagaa aatcttataa tggctagttt tgtattaatt attgtcgttg
     6061 ctttagtaca atgggggaaa ggtatttttt cagctgctgc tattgtgatt ggtatgatga
     6121 taggttatat cgtttcgcta gctctgggtt gggtaagttt taatggcgtg aaagaagcac
     6181 aactttttgc gttaccacaa cctctacatt ttggcttaga atttccaatc tccggtatca
     6241 tcggaatgtc aattgcttat ttagttacca ttgtagaatc tagcggaaat ttcttagcac
     6301 taggcaatgc aactaaaact gaaattaccg gaaaacactt acgtggaggt gtattatgtg
     6361 atggtttagg ctcagcattt gcggcaatta tgtccacaac tcccttttct tctttctcac
     6421 aaaatattgg ggtcatttca ttaaccggtg tagccagtcg ctatgttgta gcatttacag
     6481 gcgcattact tgtactagcc ggtttatttc ctgtatttgg agcattaatc gtgtctatcc
     6541 cgttacctgt gcttggcggt gcaggcttaa tgatgtttgc catgattatc gcagccggta
     6601 tccaaatgtt agataatatt caacgtagca aacgaaacgg tttaattatt gctatctcta
     6661 tcggttgtgg gctggccgta acaactcgac ctgagctatt ggataagctt ccacacttct
     6721 ttaaagaagt ttttggctca ggtattacgg tcggctcctt attagcactt attttgaatt
     6781 tagttttacc tgaagataaa ataaccaaga aagaaaataa gagtgatgaa taaatcaccc
     6841 taaaaaaaca aggacagctc acaatacaac tttgatgctg tcctttattc ccatagagag
     6901 catatttaaa tttaggttaa taaaaaaatc tgcactctat attggtgttt acctttagag
     6961 tacagatttc ttcttattct aactttctag caaacaatta gattaatgtt ccgctttggt
     7021 atcggctaac gaatccccaa tgttattatc tttatttgga ttatcctcta aagttgaact
     7081 tacatccgct gcctgttcac cacttggtgg agtggcgtta tcgtctttag ttggggtatc
     7141 tgtccaacct gatggttcac ccaccggacg gcgtgccatt aaatcatcaa tttgcttgct
     7201 gtctaaggtt tcgtatttca ataaggcatc tttcattgcg tgtaagatgt ccatattttc
     7261 ttccaaagtt tgcttcgctc gttgatagtt gcggtcaatg atcttacgca tttccacatc
     7321 aatcaatttt tgcgactctt ccgatacgcc cttaattgca cccattccat cttcattttg
     7381 atagaaaatc gggcctaatt cttttgagaa gccccattga gtaaccattg ctcgtgcaat
     7441 ttgggttgct cgatgaatat cagaggaagc ccctgtggta attttatctt caccaaaaat
     7501 caagccctca gcaattcgcc ctgcaaatag ggtggaaagt tggctttcca atttggtaaa
     7561 tgtttcgctt acacggtcac cttccggtaa gaattgagca aaacctaacg cctgaccacg
     7621 aggcacaatt gtcactttat tcagcggatc gtgttccggc attaaatagc caacaataac
     7681 gtgtccagct tcgtggtaag cagtattgat gatctctttt tccgtcattg tatttgaacg
     7741 gcgttccggc cccatattga ttttgtcacg ggcttcttcg aaatcttcca tcattaccac
     7801 acgtttattc ttacgggcag caaataaggc agcttcgttc accaagtttg ctaattgagc
     7861 accagagtaa cccggcgtac cacgagcaat ttgcatcggg tcaactccct ctgcaagcgg
     7921 tacttttttc aagtgaactt tcaaaatttg ctcacgccct ttcacgttcg gcaaatcgac
     7981 cgtgacttgg cggtcgaaac gccccggacg agttaaggca tcatctaaca catcggcacg
     8041 gttggttgca gcgatgataa tcacaccttc agagccttca aagccgtcca tctcaactaa
     8101 catttggttg agggtttgtt cacgctcatc gtgtccaccg ctgaaacctg caccaccacg
     8161 tttacgccct accgcatcaa tttcatcaat aaaaataata caaggggcat ttttcttggc
     8221 ttggtcgaat aaatcacgca cccgtgaagc acccacacct acaaacattt ccacgaagtc
     8281 ggaacctgcc attgtaaaga atggcactcg tgcttcacct gcaatcgctt tcgccaataa
     8341 ggttttaccc gtacccggag ggcctaccat caagatccct tttggaatgc gcccacctaa
     8401 tttttgaaat ttagttgggt cacgcaaaaa ctcaacaact tcgcctactt cttctttggc
     8461 ttcatcacag ccggctacat cagaaaatct ggtcttaact tcgtctgcgg tcaacatttt
     8521 ggctttattt ttcccaaagc ccattgcacc acgtcctccg ccaccttgca tttgtcgcat
     8581 ataaaacaca tagaaaccga ttaacagcaa cattgggaac caagaaatca aaatttgcga
     8641 gagcaagcca cgacgctcag caggctgacc ggtaatcact gcattggttt ttgctaaatc
     8701 tgccattaag tactcatcat acatcggcat tacggtttgg taggttgcac catcattttt
     8761 cgtcaccaaa atggtgtcat cattctgttt gaaatcgacc ccttttaaac gattttcttt
     8821 aatatcgctt aaaaaagtcg aatatggcac tgtatttgag ctaccactga aattagaatt
     8881 aaaaccttca taggctgtca tcatcacaat ggcaatcacc acccaaagta aaatattttt
     8941 aaccatatcg ttcaaggtta atcccctatc tgctgaataa aaaactgttc aagattacta
     9001 taaaagcggt cgaattttcc aatttttttg caaaatgtta ataaaaaatg accgcttgct
     9061 tgttttctag cctttataac ctgtcgctac aatataaact tctcgagacc gatctcgaga
     9121 cgcctccggt tttctgactt ttaccacact aaagagcgag cggatttctc gaaggtattc
     9181 atcaaatcct tcgccttgga ataccttaac cacaaaactg cctttggttg ctaatacttg
     9241 gcgacacata tccaatgcca attccactaa atacattgca cgaggaatat caaccgacgg
     9301 cataccgctg aaattgggag ccatatccga catcaccaca ttcactttgt cttcgcctac
     9361 tcgctcaagt aacgcattca gcaccgattc ttcacggaag tcgccttgta agaaatccac
     9421 gcctacaatc gggttcatat ctaaaatatc acaagcgatt actctgccct tactgccgat
     9481 ttgcgtcacc acatattgcg accaaccgcc cggtgcggca ccgagatcca ccaccgtcat
     9541 tcccggtttg aataaacggt cggtttgttg aatttcatct aacttaaaat acgcccgtga
     9601 acgcaatttt tgtttatgtg ctttttgcac aaattgatct ttaaaatgtt ctgcaagcca
     9661 acgtgatgaa ctcgcacttc gttttctacc catttgttct tctatcttaa ttaattcaac
     9721 gatttataat aagggttaaa ttagcaaatt caagagatgt tctaaaattt agctcggctt
     9781 attatgctta attaa
//
LOCUS       DANLXS010000088         9677 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.088, whole genome shotgun sequence.
ACCESSION   DANLXS010000088 DANLXS010000000
VERSION     DANLXS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9677
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.088"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            7..195
                     /locus_tag="PXD71_001569"
     CDS             7..195
                     /locus_tag="PXD71_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6291676.1"
                     /translation="MRNQIGISQAQLAVQIGWGQPRIANYETGNRTPSIYVAQKIVEG
                     LNELGANVRIDDVFPVGN"
     gene            216..476
                     /locus_tag="PXD71_001570"
     CDS             216..476
                     /locus_tag="PXD71_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291677.1"
                     /translation="MPRNELTKNARAIADLIHRKSATVTHKKLARAIGLSESQFSRTF
                     ADNVEMVAVIIDYLSIELADKDELLELKEKRYKRINLEEATT"
     gene            640..810
                     /locus_tag="PXD71_001571"
     CDS             640..810
                     /locus_tag="PXD71_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291678.1"
                     /translation="MKNQRNDTQKSSLWQEFEYRKRLKNLIESGLSVAEIETRSQQIY
                     EELKSECTTEAN"
     gene            785..1654
                     /locus_tag="PXD71_001572"
     CDS             785..1654
                     /locus_tag="PXD71_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL6291679.1"
                     /translation="MNAQPKQINPILKLHPKQSEAKKVSVDDGYTQIPNELLKAILRS
                     GVLGWKGSYLLATVLKTLSWHKESDWFTHSQVCEMMNIEPTKYHINQLSAARKELIRE
                     NILFEDGKETGVNLSVFEWKMVYPEKVGSSRNNRELIPEKVGNGYPEKVGNTKETITK
                     EKINNEFTNVNSVQQPETSLPKKTKSEPVDYQGVMEEFNRAVEDTPIPQIRAMSDERK
                     RSVHALAKILRKEFGGYTRQHFADYFNDFVRQASSRKDRFYFGGLDGSAWVANFGYII
                     RSKTFYKTWEDSL"
     gene            1651..3012
                     /locus_tag="PXD71_001573"
     CDS             1651..3012
                     /locus_tag="PXD71_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HDL6291680.1"
                     /translation="MSQNLKNITYEVEYLLVGALLKSGLNSKARDVLSWLEPEMFATF
                     QLGVIYEAIRKQALKDNVIDMLLLNTDYGQDFATLAEIMKNTISGANLDGYAEKVRQY
                     HQRREAQRVFLDVAGELQNARDEQLDAITSTGLAHLSKLLQRGGKVKPIDMNDLLEGY
                     LELFQERAKPSFKERLLFTGIEALDGKLGGINDTDICIVAGRAGNGKTETAITFTKNI
                     IENKGSVLFFSLEMSKEQIMDRLIASASGVNSVKLRNPEMMNDEDFARMGTAIQPLQN
                     QQLYIVDKSGLTAEEIVAIAESHIQEYGKVSAVVIDYIGLVRHGKLDGKINRTYQIGE
                     SMERFKTFCKNNHTPMILLAQLNRNADGSRPTNADLRDSGSLEQDASQIIMVHNQRNK
                     DGEPAPYTEWIVTKNRFGANGTVYMQFQQGRFVECDQAEAREYFQPKETKTSSNKRYG
                     AMQ"
     gene            3009..3545
                     /locus_tag="PXD71_001574"
     CDS             3009..3545
                     /locus_tag="PXD71_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL6291681.1"
                     /translation="MTDFDKNIKYKIIYADPPWKYKDSGCNGSAENHYTTMNIKEICD
                     LPVQDISDKDSILFLWVTYPMLPEGLKLIEAWGFNYKTIGFQWVKTNKKNKNSFFFGL
                     GRWTRGNTECCLIATKGKPSRVSNKVSQLIIEPIQHHSKKPDIVREKIVELMGDLPRI
                     ELFARNKTQGWDVWGDEV"
     gene            3636..3851
                     /locus_tag="PXD71_001575"
     CDS             3636..3851
                     /locus_tag="PXD71_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291682.1"
                     /translation="MITYKCPKCNGELEDLSINDEWGWFLDEPYRCNGHYTGRFPNIS
                     RDSTLNRTKSCGYFSKEEVKKVNGTQR"
     gene            3835..4341
                     /locus_tag="PXD71_001576"
     CDS             3835..4341
                     /locus_tag="PXD71_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL6291683.1"
                     /translation="MAHSVNDIVKAHGRRYKARLKIQMIKMQGGVLVPLNEREAEALV
                     SLKNGEQYEIEVIRTRNPAFHRKVFAFLNFCFEYWSADKTEWEFFDERKQFDTFRKNL
                     TVLAGFKEITYTLDGRMRVEAQSLSYGNMEQDEFEQVYKALINAAIKHVFNNTKDENR
                     INQLYSFF"
     gene            4391..4603
                     /locus_tag="PXD71_001577"
     CDS             4391..4603
                     /locus_tag="PXD71_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291684.1"
                     /translation="MKKNVIRYWLSVPFRIIAVLFFLVILTVRYVGYLLIYPLAFFAA
                     VGSFIVFCQWTYQEWLYYFKRGFAPI"
     gene            4649..4843
                     /locus_tag="PXD71_001578"
     CDS             4649..4843
                     /locus_tag="PXD71_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291685.1"
                     /translation="MTKFFETIAFVMLFGIIPSCLLAINTTLMNMNIKNAPWFMVIIQ
                     LGFNITCYWLWFISVMRNVI"
     gene            4971..5540
                     /locus_tag="PXD71_001579"
     CDS             4971..5540
                     /locus_tag="PXD71_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDL6291686.1"
                     /translation="MANKPPKQHKCKECGSYYIKFQSTQQVCSVKCAMAMGKRKTEAK
                     RKKSAKIDRLEQRKRLEKLKSRRDFEKDLQKIFNKFIRLRDKDLPCISCGRYHQGKYD
                     AGHYKTVGGNPELRFNEDNCHSQCVPCNRHLHGNIVNYRVNLIEKIGLERVEFLERKD
                     HPPLKLTIEQIKDLIKVYKAKCKELERVT"
     gene            5530..6003
                     /locus_tag="PXD71_001580"
     CDS             5530..6003
                     /locus_tag="PXD71_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291687.1"
                     /translation="MLPRKIKNKWLEPEKQKWIEELLRLWGAWEFGGLDLESRVNMIY
                     RLMKSAEGVKSLSSREVCNDSLGTLINEIFTVVTKRDVMLADILKQKYWFGRSERQIA
                     IYYQLRDSEGRKERRWQEIIQDKCKKAERIIADILESKISSSNSTDKLKKYQFSP"
     gene            6231..6306
                     /locus_tag="PXD71_001581"
     tRNA            6231..6306
                     /locus_tag="PXD71_001581"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6264..6266,aa:Trp,seq:cca)
     gene            6323..6508
                     /locus_tag="PXD71_001582"
     CDS             6323..6508
                     /locus_tag="PXD71_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291688.1"
                     /translation="MYNLDDAIQECREKFGEDKPYFSEAGTLTFAGCKALSRLRDEKL
                     GIKAPPNFPFPRVEDLS"
     gene            6534..6704
                     /locus_tag="PXD71_001583"
     CDS             6534..6704
                     /locus_tag="PXD71_001583"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291689.1"
                     /translation="MTEQLNTQSKSGEEDQEKSQDRYAVGWPSRRISDCVQHFRSRKI
                     YPFASNNDVKQG"
     gene            6847..7092
                     /locus_tag="PXD71_001584"
     CDS             6847..7092
                     /locus_tag="PXD71_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291690.1"
                     /translation="MKLMKDMGTQSYIWSGFSTWLAWLGEQQNLMLLSLAIGIVTAIA
                     GLIQRFDEWRRRKREDERAEELHQAKMARLRKGLSDE"
     gene            7085..7654
                     /locus_tag="PXD71_001585"
     CDS             7085..7654
                     /locus_tag="PXD71_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL6291691.1"
                     /translation="MSKSLKYGSGIVCGIAAIITLVQYQHPEIRTNQAGLEIIGNAEG
                     CRRDPYKCPADVITVGIGSTEFGGEKIDPNRIYSDKEIAERWAKDLKIAESCVNRHFN
                     GKDMNDNQFSGMTSAVFNMGCYNMRFYRNKQGQYVQTTIHKLAVNKQFEEMCHRLPDF
                     IRASGKVLNGLVIRREKEKALCLTGLVAR"
     gene            7627..7977
                     /locus_tag="PXD71_001586"
     CDS             7627..7977
                     /locus_tag="PXD71_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL6291692.1"
                     /translation="MLNWISGTIAIVILGLCVWLWGQSQMIDSLRAENRAQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKIQKIANELRNKVEMAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLHQQNNHKH"
     gene            7982..8155
                     /locus_tag="PXD71_001587"
     CDS             7982..8155
                     /locus_tag="PXD71_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291693.1"
                     /translation="MYPPAAYLVPCGRTEFSGRTYGDTVEYLVKVMGERDLCANQIDR
                     IREWQAQTKQGFK"
     gene            8547..9071
                     /locus_tag="PXD71_001588"
     CDS             8547..9071
                     /locus_tag="PXD71_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL6291694.1"
                     /translation="MTKKGEVKATSKGVGKLTDKQKRFVEEYLIDLNATQAAIRAGYS
                     EKTAYSIGEENLRKPEIRSAIQEAQNKRSERTQITQDDVLNGLLEVIAMSTGKKIVTE
                     TDVAKNDNGELVGFDIAKTKFEPAAANKALELLGKHLGMFKDKVDLINSDGSLNRPMV
                     IELIAPSLDDESTD"
     gene            9055..>9677
                     /locus_tag="PXD71_001589"
     CDS             9055..>9677
                     /locus_tag="PXD71_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL6291695.1"
                     /translation="MKAQIELPPKLLPVFAKDYRYKGAYGGRGSGKTRAFAKMSAVSA
                     YKRAMRGESGVILCGREFMNSLEESSLEEVKQAIKSEPFLANFFDVGEKYVRTKDGRI
                     SYVFSGLRHNLDSIKSKARILIAWIDEAETVSETAWRKLIPTVRENNSEIWVTWNPEN
                     RGSATDTRFRQNPPENSCIIEMSYQDNPWFPDVLEQERLNDKQRLDDA"
BASE COUNT         3159 a         1603 c         2288 g         2627 t
ORIGIN      
        1 gctgaaatac gaaaccaaat tggtataagt caagctcagt tagctgttca gattggttgg
       61 gggcaaccaa gaatagctaa ctatgaaact ggaaatcgta ccccatctat ttacgttgct
      121 caaaaaattg ttgagggatt gaacgaatta ggtgcaaacg tacgaattga tgacgttttc
      181 cccgttggca actaatttac cccaagaggc ttgcaatgcc acgcaatgaa ttaacaaaaa
      241 atgcaagagc gattgcggat ttgattcatc gaaaatccgc aacggtgacg cataagaaac
      301 tagcaagagc aatcggactg agtgaatcac aatttagccg tacatttgca gataatgtgg
      361 aaatggtggc ggtgattatt gattacttaa gtattgagct tgcggataaa gacgagttac
      421 tagagctgaa agagaaacga tataagcgaa taaatctcga agaagcgaca acatagaagt
      481 taccaacaat gaaaaatttt ttagaaaaat tcaaatcatt ctaccacagt aaaaattcaa
      541 tttttattag agtggcaatt tttatgctgc ttgcgtggtg ggtttttaac aattaaaaaa
      601 ccaccgctgg aacggtggta atttacaagg agttaccttt tgaaaaatca acgcaatgat
      661 acacaaaaaa gctccttatg gcaagagttt gaatatcgaa aacggttaaa aaatctcatt
      721 gaaagcggtt tatccgtagc cgaaattgaa acccgttcac aacaaattta tgaggagctg
      781 aaaagtgaat gcacaaccga agcaaattaa cccaatttta aaattgcacc ctaaacaatc
      841 agaggctaag aaagtgagtg ttgatgatgg ttatacacaa attccgaatg agttattaaa
      901 agcaattttg cgatcgggtg ttttaggttg gaaaggctct tacttactgg ctaccgtctt
      961 aaaaacattg tcttggcata aagaaagtga ttggttcacg cattcccaag tgtgtgaaat
     1021 gatgaatatt gagccgacaa aataccatat taatcaactt tccgctgctc gaaaagaact
     1081 tatccgtgaa aatatcttgt ttgaagatgg taaggaaaca ggggtaaatc tcagtgtttt
     1141 tgaatggaaa atggtatatc ccgaaaaagt agggagttcc cgaaataata gggaattaat
     1201 tcccgaaaaa gtagggaatg ggtatcccga aaaagtaggg aacacaaaag aaactattac
     1261 aaaagaaaaa ataaataatg aatttactaa cgtaaattct gtgcaacagc ccgagacctc
     1321 tttgccgaaa aaaacaaaat ctgagccggt cgattatcaa ggagtgatgg aagaatttaa
     1381 tcgtgctgtg gaagacacac cgattccgca aattcgggcg atgagtgatg agcgaaagcg
     1441 atcggttcac gcattagcaa aaatcttgcg aaaagaattt ggtggctaca ccagacaaca
     1501 ctttgccgat tacttcaatg attttgtccg acaggcaagt agccggaaag acagatttta
     1561 ttttggcggg cttgatggct cggcttgggt agcaaatttc ggctacatca ttcgttcgaa
     1621 aacgttttat aagacttggg aagattcgct atgagccaaa atttgaaaaa tatcacttac
     1681 gaggttgaat atttgctggt tggagcattg ctgaaatccg gtcttaactc gaaagctagg
     1741 gacgtattga gctggttaga gcctgaaatg ttcgctacct ttcaacttgg ggtgatttat
     1801 gaggctatcc gcaagcaggc gttgaaagat aacgtgatcg atatgctgtt actcaatacc
     1861 gactacggac aagattttgc cacgttggca gagataatga aaaacacgat cagcggtgca
     1921 aacttagacg gctatgcaga aaaagtccgg caatatcatc aacgccgaga agcccagcga
     1981 gtgtttttag acgttgcagg tgagttgcaa aatgcccgag acgagcagtt agatgctatc
     2041 acttcaaccg ggttagctca cctaagcaaa ttattacaac gtggcggaaa agtgaaaccg
     2101 attgatatga atgatttgct cgaaggttat ttggaattgt tccaagaacg tgcaaaacca
     2161 agttttaaag agcgtttgtt gtttaccggt atagaagcat tagatgggaa actaggcggt
     2221 attaacgata ccgatatttg cattgtagca ggacgtgccg gtaacggtaa aaccgaaaca
     2281 gccatcacgt ttactaaaaa cattatcgag aataaaggtt cggtgctgtt cttctcgctg
     2341 gagatgagca aagagcagat tatggatagg ctgattgcca gtgcaagcgg tgtaaattca
     2401 gtaaaacttc gcaatccgga aatgatgaat gatgaagatt ttgcccgaat gggaacggcg
     2461 attcagcctt tacaaaatca gcagttgtac attgttgata aaagcggatt aaccgcagaa
     2521 gagattgtgg cgattgcaga gagccatatt caagagtacg gcaaagtgag tgcggtagtg
     2581 attgattata tcggtttggt tcgacacggc aaactagacg gcaaaattaa ccgaacctat
     2641 caaatcggtg aaagtatgga gcggtttaaa actttctgca aaaacaatca cacaccgatg
     2701 attttattgg cacagcttaa ccgcaatgca gatggcagtc gcccaaccaa cgcagatttg
     2761 cgagacagtg gcagtcttga acaagatgca agccaaatca ttatggtgca taaccaacgc
     2821 aacaaagacg gcgagccagc cccctataca gaatggattg ttactaaaaa tcgctttgga
     2881 gcaaatggca cggtttatat gcaattccag cagggtaggt tcgtagaatg cgaccaggca
     2941 gaagcgcggg agtatttcca gccgaaagaa actaaaacat catcaaacaa acgctatgga
     3001 gcaatgcaat gacagatttt gataaaaata taaaatacaa aattatttac gcagatccgc
     3061 cttggaagta taaggatagt ggctgtaatg ggagtgcaga aaaccattac acaacaatga
     3121 atatcaaaga aatatgtgat ttacccgtcc aagatatttc agacaaagat tctattctgt
     3181 ttttgtgggt tacttaccct atgttacctg agggcttaaa gttgattgag gcgtgggggt
     3241 tcaactataa aacaataggg tttcaatggg tgaagacaaa taagaagaat aagaattctt
     3301 ttttctttgg cttgggacga tggactagag gtaataccga atgctgttta attgcaacaa
     3361 aaggaaagcc atcgagggta agcaataaag tgagccaatt aattattgag ccaatacaac
     3421 atcatagtaa aaagcctgac attgtcagag aaaagatagt tgagttgatg ggtgacttgc
     3481 caagaataga gttgttcgcc agaaataaaa cccaaggttg ggatgtttgg ggggatgagg
     3541 tgtgattgtt tcaagctatt gttacccaaa gtgcggtagg gttttattaa aaaggttggc
     3601 ggttgtgaag gataacgaag gttgggacgg tttagatgat tacttataaa tgccccaaat
     3661 gcaacggaga gcttgaagat ttaagtatta atgatgaatg gggttggttt ttagatgagc
     3721 cgtatcgttg caacggacac tatacagggc ggtttccaaa cattagccga gatagcacgt
     3781 taaatcgcac aaaatcctgt gggtacttta gcaaggaaga ggttaagaaa gtaaatggca
     3841 cacagcgtta atgacatcgt aaaagcccac ggcaggcgtt ataaagcaag attgaaaata
     3901 cagatgataa aaatgcaagg aggcgtttta gtccccctta atgagcgtga agctgaagca
     3961 ttggtatcac ttaaaaatgg cgagcaatat gaaattgagg ttattcgcac tcgcaatcca
     4021 gcctttcatc ggaaggtctt cgccttttta aatttttgct ttgaatattg gtcggcagac
     4081 aaaaccgaat gggagttttt tgatgagagg aaacagtttg atactttccg aaaaaatctg
     4141 actgttctgg caggatttaa agaaatcaca tacaccctag atgggcgtat gcgtgtagaa
     4201 gctcaaagtc ttagttacgg aaatatggaa caagacgagt tcgagcaggt ttacaaagca
     4261 ttgattaatg cagctatcaa gcacgttttc aacaatacca aagatgagaa taggataaat
     4321 cagttgtatt cgttttttta attaaggcga aatcgccata tttaattagg tttaattgca
     4381 ggagattaaa atgaaaaaga atgttattag atattggctt agcgtgccat tcagaattat
     4441 tgcagtgctt tttttcttag tcattcttac tgttagatat gtaggttatt tattgattta
     4501 tccattagca ttctttgcag cagtcggtag ttttattgtt ttctgtcaat ggacatatca
     4561 agaatggctt tattatttta agcgcggttt tgctccgatt tagtgtgtgt ttattaattc
     4621 cctcgaattc gggggaagga gaagtaagat gacaaaattt tttgaaacta tcgcattcgt
     4681 gatgttattt ggcattattc caagctgttt gttggcgatt aatactactt taatgaatat
     4741 gaatattaaa aacgcaccgt ggtttatggt gattattcag ttaggtttta atatcacttg
     4801 ttattggttg tggtttattt ctgttatgcg gaatgttatt taaggagatt aataagtgag
     4861 ccagtgcaat gaactgactg taattttaat cgccatcgct attcttagtg tatgtgccgg
     4921 ttcactcttt tttattgagt ggtttgataa gagagatggt gagggttgga atggctaaca
     4981 aaccacctaa acaacacaaa tgcaaagagt gcggcagtta ttacatcaaa tttcaaagca
     5041 cgcagcaggt ctgctctgtt aagtgtgcta tggcaatggg taagcgtaag acagaggcga
     5101 aacgcaaaaa aagtgcaaaa atcgaccgct tggagcaacg gaagcggctg gaaaaattga
     5161 aaagccgtag agattttgag aaagatttac aaaagatatt taataaattc attcgcttgc
     5221 gtgataaaga tttaccctgt atttcctgtg gccgctatca tcaggggaaa tatgatgcag
     5281 ggcattataa aaccgttggt ggtaatcctg agctacgttt taatgaagat aattgccaca
     5341 gtcaatgtgt gccgtgtaac cgacatctgc acggcaatat tgtgaattat cgagtaaatc
     5401 tgattgagaa aatcgggctt gagcgtgtgg aatttttaga gcgtaaagac cacccaccat
     5461 taaaacttac tatcgaacaa atcaaagatt taatcaaagt ctataaagcg aagtgcaagg
     5521 agcttgagcg tgttacctag aaaaattaaa aacaagtggt tagagcctga aaagcagaaa
     5581 tggatcgaag agttgctaag gttatggggg gcgtgggaat ttggcggatt ggatttagaa
     5641 agtcgggtaa atatgattta ccggctgatg aaatctgcgg agggggttaa aagtctttca
     5701 tctagagagg tttgtaacga tagcctcggc acgctgatca atgagatttt tacggtagtc
     5761 accaaaagag acgtaatgtt agccgatata ctcaaacaaa aatactggtt cgggcgttcg
     5821 gagcggcaaa tcgctattta ttatcaacta agggacagcg agggtagaaa agaaagacga
     5881 tggcaagaaa ttattcaaga taaatgcaaa aaagcggaaa gaattattgc agatattctt
     5941 gaaagcaaga tttcatccag taattctaca gataaactga aaaaatatca attttcccct
     6001 tgattttagt gcgtaacaag tgtatatttt gtgctaatgg tggacgtagt ataaatgatg
     6061 tttaccaatg ttagttaaag acgcagcctg aagtaatatt cagttcggag gagaataact
     6121 agaaacgacg tcgagtatag ttatttcagc gtttgcgtgg cagtatgccg accgaattag
     6181 tttaacagcc ctgagcagaa atgcttgggg cttttttgtt tacggatttt aagggtgtag
     6241 ctcagttggt agagcagcgg tctccaaaat cgttggttgt tggtttgagt ccaaccaccc
     6301 tttccaaatg cgagtaaatg ttatgtataa ccttgatgat gcaattcaag aatgcagaga
     6361 aaaatttggt gaagataagc cttatttttc tgaagctgga acacttactt ttgctggttg
     6421 caaagccctc tctcgattaa gagatgaaaa gttaggaatt aaggctccgc caaatttccc
     6481 tttcccaagg gttgaagatc tatcttagct acaaaaatca atgagggatt gctatgacgg
     6541 aacaactaaa tactcagtca aaaagcggtg aagaagatca agaaaaatct caagatcgct
     6601 atgccgtggg ctggccatct cgacgtatat cagattgcgt tcagcacttc cgaagtagga
     6661 aaatttatcc atttgccagt aataatgacg tgaagcaagg ttaaatgctt ctggtggtac
     6721 agagtaaatt caatcatttt taccctctgc tagtttattt gttggggaac agtattctag
     6781 cagaattttt aaccaaagct cagtctcacg actgggcttt tttattgcct cgaaacgggg
     6841 tggagtatga agttaatgaa agatatggga acgcagagct atatttggtc gggatttagc
     6901 acttggctgg cgtggctcgg cgaacagcag aatttaatgt tacttagtct tgctatcggt
     6961 attgttactg ccattgcagg gcttattcag cgatttgatg aatggcgtag gcgtaagcgt
     7021 gaagatgagc gagcggaaga gcttcatcaa gcgaaaatgg caagattgag aaaggggtta
     7081 agtgatgagt aaaagcctta aatatggcag tggtatcgtt tgtggtatcg ctgccattat
     7141 tacattagtc caatatcaac atcccgaaat ccgcactaac caagctggat tagagattat
     7201 cggtaatgct gagggttgca gacgtgatcc gtataaatgc cctgccgatg taatcacggt
     7261 tggcattggc tcaacggagt ttggcggtga aaagattgac ccaaatcgta tttattctga
     7321 caaagaaatt gctgagcgtt gggcaaaaga tttgaagatt gcggagagtt gtgtcaatcg
     7381 tcattttaat ggtaaagaca tgaacgataa tcaattttcg ggaatgacgt ccgctgtatt
     7441 taatatgggt tgttataaca tgagatttta tcggaataaa caaggtcaat atgtgcagac
     7501 taccatccat aaactcgcag tgaataaaca atttgaggag atgtgtcatc gactacctga
     7561 ctttattcgt gccagtggta aggtattgaa cggattggtt atccgccgtg aaaaggagaa
     7621 agcattatgc ttaactggat tagtggcacg atagccattg tgattttggg cttgtgtgta
     7681 tggttatggg gtcagtcaca gatgatagat agcttaagag ctgagaaccg cgcacaagcc
     7741 caaaccattg agcagcagca aaaagccaat caacggctaa ccgatagctt agagcaagag
     7801 cgacaagcgg tcgaaaaaat ccaaaaaatt gcaaatgagc tgcggaataa agtggagatg
     7861 gccaagaatg aaattacatc aatactggca caagacagtt gtgctaaagc tgatttgcct
     7921 aatggcgttg ctgatagcat taagcggctg caccagcaga acaatcacaa gcactgagta
     7981 tttgtatccg ccggcagctt accttgtgcc gtgtgggcga acagaattta gcggtagaac
     8041 ctatggcgat acggttgagt atctcgtcaa agtgatggga gagcgtgatt tgtgtgctaa
     8101 tcaaattgac cgtatcagag aatggcaagc acaaaccaaa caagggttta aatagctaat
     8161 tatctaatta accgctattg aaaatgggag cgtaacagct ccctttttgt tttgatggaa
     8221 agtaaatatt acataattta gctatggttt cggggttggt tggataaaac accgaggaaa
     8281 tacctaagtg atgtgattta tataaaacaa aaccccgaac atttgcggtg ttcgaggttt
     8341 ttttattacc cattgaaacg gaatgagtaa caagttatgg aaattattac atttttaacc
     8401 gtaaccatca aggagattct tatggaatat ggtttatggc aaataagcct agcagtaaca
     8461 gtccctattt tggcgttcgt gtcgccaaag ctgattaatg ctattgctaa tctattaaat
     8521 gcaatgaagt aaaggaattt aacccaatga ctaaaaaagg cgaggttaaa gccacgtcta
     8581 aaggcgtggg taaattaacc gataaacaga aacgatttgt agaagagtat ctgattgact
     8641 tgaatgcgac tcaagctgcg attagagctg ggtacagcga aaaaacagca tactcaatcg
     8701 gagaagagaa cctgagaaaa cctgaaatca gaagtgcaat tcaggaggct caaaataaac
     8761 gctcggagag aacccaaatt acacaagatg atgtgctaaa tgggttgctt gaagtgattg
     8821 caatgagtac tggtaaaaaa attgtgacag aaactgatgt tgcaaaaaat gacaatggtg
     8881 agttggtcgg ttttgatatt gcaaaaacaa aattcgaacc tgccgccgca aataaagctc
     8941 ttgagttact cggtaaacat ttaggaatgt ttaaagacaa ggttgattta attaattctg
     9001 atggttcact gaatcgaccg atggtgattg aattaattgc tccgagttta gacgatgaaa
     9061 gcacagattg aactaccacc taaactgttg cctgtttttg ccaaagatta tcgttacaaa
     9121 ggtgcttatg gtgggcgagg ttcaggcaag actcgggcat ttgccaaaat gtcggcagtg
     9181 tctgcttata aaagagcaat gcgaggggaa agtggcgtta ttctttgcgg tcgtgagttt
     9241 atgaactcat tggaagaaag ctctcttgaa gaagttaaac aggcgatcaa gtctgagcct
     9301 tttttagcaa atttttttga tgtcggcgag aagtatgttc gcactaaaga tgggcggatt
     9361 tcttatgtgt tttccggact tcggcataac ttagatagca tcaaatctaa agcccgtatt
     9421 ttgattgcgt ggatagatga agcagaaaca gtaagcgaaa ctgcttggcg gaaattaatt
     9481 ccaaccgtgc gtgagaataa ttccgaaata tgggtaactt ggaacccgga aaatagaggg
     9541 agcgcaacag atactcgctt caggcaaaat ccacctgaaa acagctgcat aattgaaatg
     9601 agctaccaag ataatccttg gtttcctgat gtgctcgagc aagagcggct aaatgacaaa
     9661 cagcggcttg atgatgc
//
LOCUS       DANLXS010000089         9622 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.089, whole genome shotgun sequence.
ACCESSION   DANLXS010000089 DANLXS010000000
VERSION     DANLXS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.089"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            9..287
                     /locus_tag="PXD71_001590"
     CDS             9..287
                     /locus_tag="PXD71_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291696.1"
                     /translation="MEILFILLGLIGFGALLAIGFTTFAAIFGVVTTVFLSLLSLLAN
                     MVGLSIIVILISLYVLLPLNTFILIALIFSAILGFLCWYHSDRQSVNQ"
     gene            complement(338..796)
                     /locus_tag="PXD71_001591"
     CDS             complement(338..796)
                     /locus_tag="PXD71_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL6291697.1"
                     /translation="MSEPIEQVKTAFDSLLNNISKPRRRLMYQQIGRELARSQRRRIK
                     AQQNPDGSAYEPRKKPKKGVKSKIKSGKMFDKITQPRFMRLRLESEGVSLGYEGGDAV
                     IARIHQQGLIGRVRKDWDLKVKYASRELLGFTDDDLQMIEDYMINILAGS"
     gene            complement(789..1274)
                     /locus_tag="PXD71_001592"
     CDS             complement(789..1274)
                     /locus_tag="PXD71_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6291698.1"
                     /translation="MIKPDRLRAVLTQTIDIFQANPEKLILQYDKGKIKSKGSQSHSF
                     EYHYDLELIVVDFPYHPDVLFVPVLNFVRNEQWELLQNPELQDKIEFEIDHNNHESYD
                     IYIRLPLTERVIVKEQDGHLTATHADEPNLADVSPFTRLTEYEVYLKDELIYQWAEPN
                     E"
     gene            complement(1271..1492)
                     /locus_tag="PXD71_001593"
     CDS             complement(1271..1492)
                     /locus_tag="PXD71_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDL6291699.1"
                     /translation="MSDQIDRANELAEKAREAALAKILQNQTACTSLFECEDCGEPIP
                     EKRREMVIGCTRCIECQTIYEHKQKGYRR"
     gene            complement(1641..2096)
                     /locus_tag="PXD71_001594"
     CDS             complement(1641..2096)
                     /locus_tag="PXD71_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291700.1"
                     /translation="MIVKLCQMTNKHFSPLMIVLVLVGVLANFVQFRQNESLKADLQA
                     RTTQNQLIQADNRNLANQLENSQIQLEAYQKQVDDLNQKVLAKMKQAEQRSNEILNEL
                     EKHKKWADSAVPPSVGKLLNQRKRAVSHPQIEPDNLSEKAGVPNTGNRD"
     gene            complement(2093..2659)
                     /locus_tag="PXD71_001595"
     CDS             complement(2093..2659)
                     /locus_tag="PXD71_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL6291701.1"
                     /translation="MSKSVKVGVFVCSVAAIIGTVKQYYSSEIRTSETGLAIIGNAEG
                     CRRDPYKCPADVITVGIGSTEASGEKINVNHKYTDKEIAERWIKDLKIAERCINRYFN
                     GDKMNENQFSAMVSAAFNMGCYNLRFYPNENGKYIQTTIHKYAMAKDFKAMCNRIPDF
                     NRSGGKVLRGLKIRREKEKALCLGQEVK"
     gene            complement(2652..2858)
                     /locus_tag="PXD71_001596"
     CDS             complement(2652..2858)
                     /locus_tag="PXD71_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HDL6291702.1"
                     /translation="MHKTEQASYFGAIFGFFGSLTLSDWGVVVSILVAILTLLLNWAY
                     RAKEYKLKEREFELKLQNQGKKNE"
     gene            complement(2864..3076)
                     /locus_tag="PXD71_001597"
     CDS             complement(2864..3076)
                     /locus_tag="PXD71_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL6291703.1"
                     /translation="MNTLIARQDDTLDELIFRHYGQTAGLVEQALEYNPELAHLPRLP
                     IGTVVTMPDIESRFATVAKSSVQLWD"
     gene            complement(3073..3588)
                     /locus_tag="PXD71_001598"
     CDS             complement(3073..3588)
                     /locus_tag="PXD71_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDL6291704.1"
                     /translation="MEQRNHVISIPKVNGYESSKNAIKPTAESDETITNNGFFPNIEL
                     LEVRNAMRIDGTVINERLKQAVIEAMATVNADLKAYRLNAEQAQKANLQACDDEQING
                     ESVLVYKYKRAVYCLAVANLYERYRSYDSTKDGHDKAEELESTAGDLKRDYHFAVRDI
                     LGENRMISELI"
     gene            complement(3700..4389)
                     /locus_tag="PXD71_001599"
     CDS             complement(3700..4389)
                     /locus_tag="PXD71_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDL6291705.1"
                     /translation="MRMSPARAHFLRKTAESESATPNRENLEGLQGYDLVLAQLKAHQ
                     RQLKKIQSIERKIEFKRNNFEQYRPWVEGSLAKGSGVQDTVITTMLIWSIDIGDYPTA
                     LAIAQYVLMHDLAMPEQFERTPATALVEELAEAAKKARDQKQPFEAAVLKQANDLTAE
                     FDMPDPVRAKLLRELGELIQEAEPQTALEHYQRAIALDPQCGAKGLRDKLEKQLAKQA
                     EEPKPAEEQNE"
     gene            complement(4399..5427)
                     /locus_tag="PXD71_001600"
     CDS             complement(4399..5427)
                     /locus_tag="PXD71_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDL6291706.1"
                     /translation="MKDLTLEKYNAYLARQAELNNLPFNALATGIKFTVQPSVQQKLY
                     EKVRESSDFLKSISFVFVDEQTGETLGLDSAHTVASTTDTSGDGERKTTSIAKLVKQT
                     YHCQQINFDTHINYKQLDMWAKFPDFQQKVANVAAKQRKRDLIMIGFNGTSRAATSDR
                     NSNPLLQDVAKGWLQKMREDAKERVMNGESTDNQVLVGKGQEYANLDALVMDATEELI
                     DEWHRDDTDLVVITGRKLLADKYFPIVNQQNAPTEELAADIVISQKRIGGLKAVRVPF
                     FPANAILITKLENLAIYVQEGTTRKHIENVPKKDRIETYESENIDYVVEDYGCAALIE
                     NITLKDKE"
     gene            complement(5441..6268)
                     /locus_tag="PXD71_001601"
     CDS             complement(5441..6268)
                     /locus_tag="PXD71_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDL6291707.1"
                     /translation="MTTKSKWFVVATEGATTDGREISREWLTQIEENYDPKNNYGARI
                     NLEHFRWRWFEKDDPHSYSYGDVLAVKTEEREDGKLQLLAEIAPTEALVKLVKDKQKV
                     YTSVEIDLNFADTGEAYLVGLAVTDTPASLGTEYLTFCAGATHNPLADRKQKPENLVS
                     EAVEANLEFTAEPEKGAWLEKFKAMFTKAKTDNDGKFAEHEKAMELLAEQFSKVSTEN
                     DRLQTELSTLQIEFTELQKQAGEFAEKFAKLEQEPSANYTPRPKATGAKAGDVQTDF"
     gene            6418..8220
                     /locus_tag="PXD71_001602"
     CDS             6418..8220
                     /locus_tag="PXD71_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDL6291708.1"
                     /translation="MENENTITEYEVLAANPLHDKREAQSKYWAGYTVTEISRQLNIP
                     VSTIASWKKREKWDEISPVGRVEATLESRLNLLIMKESKNNNDYKEMDALRRLLESTA
                     RIKKYSNGGGNEADLNPNIKNRNKGDRKKPEQNAISEEQAELLINGFLDGMFHYQKKW
                     HEAGLTHRIRNILKSRQIGATYYFAHEALVDALVTGRNQIFISASKKQALQFRAYIVA
                     YAKRVADVELKGETITLPNESQLIFLGTNSKTAQSYHGNLYFDEIFWVNRFEEIRKVA
                     AGMASQKQYRITYFSTPSSITHSAYLLWSGKLFNRKRPKAEQVEIDISHANLKNGKKC
                     GDGQWRQIVNIYDAEAGGCNLFDIEQLKLENSPDEFEQLFMCEFIDDNQSVFKFTMMQ
                     RCLVDSMEVWRDYVFTDGYQRPFGNKEVWVGYDPSYTGDRSALVVIAPPKVDGGKFRL
                     LEYRTFKGADFAEQAAEIVAICAKYNVTRLAIDTTGLGVGVYEIVKKERPDAVALTYN
                     VELKSKMVLKGLDIISKGRFEFDSMHAVEVGASFMAIKKQITNSGRQVTYVADRSEEA
                     SHADLAWACLQVFINEPFDGKLEETTGTVDFLDD"
     gene            8229..9269
                     /locus_tag="PXD71_001603"
     CDS             8229..9269
                     /locus_tag="PXD71_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL6291709.1"
                     /translation="MKKQLRKNLTQNDRLQPQAQTEIFSFGDPIPVLDRADILNYLEC
                     SAMYEKWYNPPMSFDGLAKSLRSSTHHESAIITKANILLSTCEVDSRYLSRRDLSSFV
                     KDYLVFGNAYFEVVRNRLGQVQRIESPLAKYVRKGLEAGQFYYVPQRFDHQEHEFAKG
                     SIYHLLEPDINQDIYGLPQYLSALQSAWLNESATLFRRKYFLNGAHAGFVFYMSDASQ
                     KQEDVENIRQQLKQSKGVGNFKNLFVHAPNGKKDGIQIIPIADVSAKDEFFNIKNVSR
                     DDVLAAHRVPPQLMGIIPNNTGGFGNVADAAEVFFITEIEPLQERLKEFNQWLGQEVI
                     KFKPSKLLQRTQ"
BASE COUNT         2400 a         2127 c         2134 g         2961 t
ORIGIN      
        1 gaaaatctat ggaaatctta tttatcttac ttgggttaat tggtttcggt gcattattgg
       61 caataggttt tacaaccttt gctgccattt ttggtgtagt tacaacagta tttttaagcc
      121 tgctttcatt attggcaaat atggttgggc tctctattat cgttattctg atctcacttt
      181 atgtattgtt accactcaat acttttattt tgatagcatt gattttttca gctattttag
      241 ggtttctttg ttggtatcat tcagatagac aaagcgttaa tcaataactc aagcggtcaa
      301 aattgaccgc ttttttgcaa aagtttctat tcatcattta gctaccagcc aaaatattaa
      361 tcatataatc ctcaatcatc tgcagatcgt catcagtaaa acctagcaac tcacggctgg
      421 catatttcac ttttaaatcc caatctttcc gcactcgtcc gattaagcct tgttggtgga
      481 ttcgggcgat gaccgcatca ccaccctcat aacctaagct gacaccttcg ctttcaaggc
      541 gtagccgcat aaaacgtggc tgggtaattt tgtcaaacat cttgcccgat ttaattttgc
      601 tttttacccc tttcttcggc tttttgcgtg gctcgtatgc cgagccgtct gggttttgtt
      661 gtgctttaat ccgtctgcgt tggcttcggg caagctctcg cccgatttgt tgatacatca
      721 accgtctgcg aggtttgctg atattattca gtaagctatc aaaggcggtt tttacttgtt
      781 cgatcggttc actcatttgg ctctgcccac tgataaatca gctcgtcttt gaggtaaacc
      841 tcatattcgg ttaaacgggt aaatggcgaa acatcggcaa ggttcggttc atcggcgtgg
      901 gttgcagtta ggtgtccgtc ttgctctttc acgatcaccc gctcggtaag cgggaggcgg
      961 atataaatat cgtagctctc gtgattgttg tggtcgattt caaattcgat tttgtcttgt
     1021 agttccgggt tttgcagtag ctcccattgt tcatttcgca caaaatttag cactggcaca
     1081 aacaacacgt cggggtgata cggaaaatcc accacgatta gctctaaatc gtagtggtat
     1141 tcaaacgaat ggctttggct ccctttgctt ttaattttgc ctttgtcata ttgcaaaatc
     1201 agcttttcgg ggttagcttg gaatatatca atggtttgtg tgagcacggc tcgcaagcga
     1261 tcaggtttaa tcatctgcgg tagccttttt gcttatgctc gtaaatggtt tggcactcaa
     1321 tgcaacgggt gcaaccaata accatttctc ggcgtttttc ggggattggc tctccgcaat
     1381 cctcacactc aaaaaggctg gtgcaagcgg tctgattttg cagaattttt gcaagtgccg
     1441 cctctctggc tttttcggct aattcgttgg ctcggtcgat ttggtctgac atagtcgtcc
     1501 ttattaaaaa aggcgatcac tcgccttggt tgattttgtt gtattgctcg atgcaatgct
     1561 cgaacgattc gatgatgact tggcatacct caatggtgtt gagtcttttg tctagtgctt
     1621 tgacaagatc accgttggtg ttaatctcgg ttgccggtgt ttggcactcc agccttttcg
     1681 gacagattat cgggttcgat ttgagggtgt gataccgcac gctttcgttg gttgagcagc
     1741 ttgccaacgc taggaggcac ggcagaatca gcccactttt tatgcttttc cagttcgtta
     1801 aggatttcat tacttcgttg ctccgcttgt ttcattttgg ctaacacttt ctgattgagg
     1861 tcgtccacct gtttttgata cgcctcaagc tgtatttggc tgttttcaag ctgattggct
     1921 aaattgcggt tatcggcttg aatcagttgg ttttgcgtgg tgcgtgcctg taagtctgct
     1981 ttcaggcttt cgttttggcg aaactgcacg aaattggcta atacgccaac tagcaccagc
     2041 acaatcatta gcgggctgaa atgcttattg gtcatttggc agagtttgac aatcatttaa
     2101 cctcctgccc gagacacagg gctttttctt tttctcggcg gattttcaaa cctcttagca
     2161 ctttacctcc cgagcggtta aaatcaggta tcctattaca catcgcttta aaatccttag
     2221 ccatagcata tttatggatt gttgtctgaa tgtatttacc attttcattt ggataaaatc
     2281 taaggttgta acatcccata ttaaatgctg ctgataccat cgccgaaaac tgattttcgt
     2341 tcattttgtc gccattaaaa taacgattga tacacctttc agctatttta agatctttta
     2401 tccaacgctc ggcaatctct ttatcggtgt atttgtgatt gacattaatt ttctcaccgc
     2461 ttgcctcggt tgagccaatg ccaaccgtga ttacatcggc agggcattta tacggatcac
     2521 gtctgcaacc ctccgcattg ccgataatgg ctaagcctgt ttcgctggtg cggatttcgg
     2581 agctgtaata ttgctttacc gtcccgataa tagctgcaac ggagcagaca aatacgccaa
     2641 cttttacact tttactcatt ctttttccct tggttttgta gctttagctc aaattctcgc
     2701 tctttcagtt tgtattcttt ggctcggtat gcccaattaa gtaataaggt taaaatagct
     2761 accagaatac tcactaccac cccccaatca gatagggtga ggctaccaaa aaagccaaaa
     2821 attgccccaa aataggaggc ttgttcagtt ttgtgcatat tttttaatcc caaagttgca
     2881 cgctggattt cgccaccgtt gcaaaacggc tctcaatatc cggcattgta actacagtgc
     2941 cgatcggcaa acgtggcaaa tgagccagtt cggggttata ttctaaggct tgctcgacta
     3001 agcctgccgt ttgcccgtag tgccgaaaga tcagctcgtc taaggtgtcg tcttggcgtg
     3061 caatcaaggt gttcatatca attcactaat cattcggttt tcgcccaaaa tatcacgcac
     3121 ggcaaagtgg taatctcgtt tcaaatcacc ggcggtactt tctagctctt cggctttgtc
     3181 gtggccgtct ttggtgctgt cataactgcg gtagcgttcg taaaggttcg ctactgcaag
     3241 gcaatagacc gctcgtttat atttgtaaac cagcacgctt tcgccgttga tttgctcatc
     3301 atcacaggct tgcaagttcg ctttttgtgc ttgctctgca ttgaggcgat aggcttttaa
     3361 gtcggcattg actgttgcca ttgcctcaat caccgcttgt tttagccgct cattaatgac
     3421 cgtgccatca atccgcattg catttcgcac ctccagtagc tcaatgttcg gaaaaaagcc
     3481 attattggtg atagtttcat cagattctgc ggtgggcttg atcgcatttt tgctggattc
     3541 atagccattc actttcggga tactaataac gtgattgcgt tgttccataa ggtttccgta
     3601 aggtaagggg tggggatttt aaaagggtgc aaaatgaata aatcaccctt taaaatccgc
     3661 cccttggctg gcgtggatac gctctatttc ggctttcgcc tactcattct gttcttctgc
     3721 tggtttcggc tcttcggctt gtttggcaag ttgcttttct aacttgtctc gcaagccttt
     3781 tgcaccgcat tgagggtcga gggctatcgc tctttgatag tgttcaaggg cggtttgtgg
     3841 ctctgcctct tggattagct cgcctagctc acgcagtaat ttggctcgca ctgggtcggg
     3901 catatcaaat tcagcggtga ggtcgtttgc ctgtttcagc acggctgcct caaacggctg
     3961 tttttggtct cgggctttct ttgccgcctc tgcaagctct tcaaccaagg cggttgccgg
     4021 tgtgcgttca aactgctccg gcattgccaa atcgtgcatt aaaacgtatt gagcaatcgc
     4081 aagggcggtt gggtaatcgc caatatcaat cgaccaaatc agcattgtgg tgataaccgt
     4141 gtcttgcaca ccggagcctt tggcaaggga gccttccacc caaggtcggt attgctcaaa
     4201 attgttgcgt ttaaactcaa tctttcgctc aatcgactgg attttcttta attggcgttg
     4261 atgggcttta agttgggcta aaactaagtc atagccttgt aagccttcaa ggttttctcg
     4321 gtttggggtt gcagactcac tttctgcggt tttccgcaaa aaatgagccc gagccggact
     4381 cattcgcata gttccacctt actctttatc tttgagtgta atgttttcga ttaatgccgc
     4441 acagccataa tcttcgacaa cgtagtcgat attttctgat tcgtaggttt caatacggtc
     4501 ttttttcggc acgttctcaa tatgcttgcg ggttgtcccc tcttgcacat agatggctaa
     4561 attttccagc ttagtgatta aaatcgcatt cgccgggaag aacggcacac gcaccgcttt
     4621 taagccccca atgcgttttt gtgagatcac aatatcagcg gcaagttctt cggttggtgc
     4681 gttttgctga ttcacaatcg ggaaatattt atccgcaagc aacttgcgac cggtaatcac
     4741 cactaaatcg gtgtcatcac gatgccattc atcaataagt tcttcggtgg cgtccattac
     4801 gagtgcgtca agattggcat actcttgacc tttgccgact aaaacttggt tgtctgtgct
     4861 ttcgccattc attacacgct ctttggcatc ttcacgcatt ttctgcaacc agccttttgc
     4921 cacatcttgc aataacgggt tgctgttgcg atcactggtt gccgcacgac ttgtgccgtt
     4981 aaagccgatc ataattaagt cacgtttgcg ttgttttgcc gcaacattcg ccactttttg
     5041 ctgaaaatca gggaatttcg cccacatatc caactgttta taattgatat gggtgtcaaa
     5101 attgatttgt tggcagtggt aggtttgttt taccaacttc gcaatagagg tggttttgcg
     5161 ttcaccgtca ccgctggtat cggtggtgct ggccacggtg tgagctgaat ctaaacctaa
     5221 ggtttcaccg gtttgctcat caacaaacac aaagctgata gattttaaga aatcagagct
     5281 ttcacgcacc ttttcataca gcttttgctg taccgatggt tgcactgtga atttaatgcc
     5341 ggttgccaat gcattaaacg gcaagttgtt taattcagct tggcgtgcaa ggtaggcgtt
     5401 atatttttca agggttaaat ctttcattcg tgatcctcaa ttaaaaatcg gtttgtacat
     5461 cacctgcttt cgcaccagtg gcttttgggc gtggggtgta attggcagac ggttcttgtt
     5521 ccagttttgc aaatttttcg gcaaattcgc ccgcttgttt ttgcaattcg gtaaattcga
     5581 tttgcaatgt gcttagttca gtttgcaagc ggtcgttttc tgtcgaaact ttgctaaatt
     5641 gctcagctaa cagttccatt gccttttcgt gttcggcaaa tttgccgtca ttgtcggttt
     5701 tggctttggt aaacatcgcc ttaaatttct ccagccacgc acctttttca ggttcagcag
     5761 taaattctaa attcgcctct actgcctcac tcacaaggtt ttccggcttt tgcttgcggt
     5821 ctgctagtgg gttatgggtt gcaccggcac aaaatgtgag gtattccgtg ccgaggcttg
     5881 ccggtgtatc agtcaccgcc aaaccaacta aatacgcctc gcctgtgtcc gcaaaattaa
     5941 ggtcaatttc cacagaggta taaactttct gcttgtcttt gactaacttc actaaggctt
     6001 cggttggggc gatttctgcc agtaactgta atttgccgtc ttcacgttct tctgttttaa
     6061 ccgctaacac atcgccatag ctgtaagaat gcgggtcatc tttctcaaac caacgccagc
     6121 ggaaatgctc aaggttaata cgagcaccat agttattttt cgggtcgtaa ttttcttcaa
     6181 tctgcgtgag ccattcacga ctaatttcgc gtccatctgt agttgctcct tccgttgcta
     6241 ccacaaacca tttggatttt gttgtcatct gtttttcctt gctctaaagg ggaggggtaa
     6301 aaatcggatt tatgatcttg ttttgtgccg gcggtttcaa tgtgtggtac gtgttaaatc
     6361 gggtttcaca gcctcaagca aatgcttaaa caaggtggat ttttgataat cgcacctatg
     6421 gaaaatgaaa atacaatcac tgaatacgag gtgttggctg ccaaccctct acacgataaa
     6481 cgagaggctc aatcaaagta ttgggcaggt tacaccgtga ccgaaatttc tcgtcagcta
     6541 aatattcccg tctctaccat tgcaagttgg aaaaagcgag agaaatggga cgaaatcagc
     6601 cccgttggac gtgtggaagc cacactggaa tcacggctga atttgctgat tatgaaagag
     6661 agcaaaaaca ataacgacta caaagaaatg gatgctttac gtcggttgtt ggaaagtaca
     6721 gcacgaatca aaaaatattc taacggcgga ggtaatgaag ccgacctcaa cccaaatatt
     6781 aaaaaccgta ataaaggcga ccgcaaaaag cccgagcaaa atgccatttc cgaagagcaa
     6841 gccgaactgt tgataaacgg ctttttagac ggaatgttcc attatcagaa aaaatggcac
     6901 gaagctgggc tgactcatcg aatccgcaat attcttaaaa gtcgtcaaat tggagcgact
     6961 tattattttg ctcacgaggc gttagttgat gcgttagtaa ccggtagaaa tcagattttt
     7021 atttccgcct cgaaaaaaca ggcattgcaa tttagagcct atattgtggc atacgccaaa
     7081 cgggttgccg atgtggagct aaaaggcgaa accattacct tgccgaacga gtctcaacta
     7141 attttcttgg gtacaaactc gaaaacggca cagtcttacc acggcaatct ctattttgat
     7201 gaaatctttt gggtaaaccg ctttgaggaa atccgcaaag tggcagcggg tatggcgagc
     7261 caaaaacaat atcggattac ttatttttct accccttcga gcattaccca ttccgcctat
     7321 ctgctttggt cgggcaagct gtttaaccgc aaacgtccga aagcggaaca ggtggaaatt
     7381 gatatttctc acgccaactt gaaaaacggc aaaaaatgcg gagacggcca atggcgacaa
     7441 atcgtgaata tctacgatgc cgaggcaggt ggctgtaacc tgtttgatat tgagcaactc
     7501 aaactggaaa actcgcccga tgagttcgag cagctcttta tgtgtgagtt tattgatgat
     7561 aaccagtctg tgttcaagtt cacgatgatg caacgttgcc ttgtggactc gatggaggtg
     7621 tggcgagatt atgtattcac ggacggctac caacgcccat tcggcaataa agaggtatgg
     7681 gttgggtatg acccgtctta caccggcgac cgctcggcgt tggtggtaat cgctccgccg
     7741 aaagtggacg gcgggaaatt ccgactatta gagtatcgca cttttaaagg ggctgatttt
     7801 gccgaacaag cggcagaaat tgtcgctatt tgtgcaaaat ataacgtgac cagactggcg
     7861 attgatacca cagggcttgg tgtcggtgtt tatgaaatcg tcaaaaaaga acgaccagat
     7921 gcggtggctc ttacttacaa cgtagagctg aaatccaaga tggtactaaa aggcttggat
     7981 attatcagca aaggacgatt tgaatttgac tcaatgcacg ctgttgaggt tggtgcgagt
     8041 tttatggcaa ttaaaaaaca gatcaccaac agcggacggc aagtgactta tgtggcagac
     8101 cgttcggaag aggcaagcca cgccgacctt gcgtgggctt gcttgcaggt atttattaat
     8161 gagccgtttg atgggaaact ggaagaaacg accggcacag tagatttttt agatgattag
     8221 gacaaaaaat gaaaaaacaa ttgcgcaaaa atttaaccca aaatgaccgc ttacaaccgc
     8281 aagcacaaac cgaaattttc agttttggtg acccgattcc ggtgttagac cgtgccgata
     8341 ttctcaatta tttggagtgt tcggcgatgt atgagaaatg gtataaccca ccgatgagct
     8401 ttgatggctt ggcgaaatcg ttaaggtcat caacacacca cgaaagtgcg atcatcacca
     8461 aagcaaatat tttgctttcc acttgcgagg ttgatagccg ttatttatca cgccgtgatt
     8521 tgtcatcgtt tgttaaagat tatttagtat ttggcaacgc ctattttgag gtggtgcgaa
     8581 atcgccttgg gcaggtacag cgaatcgaat cacctttggc aaaatatgtg cgaaagggtt
     8641 tagaggctgg tcaattttac tatgtgccac aacgattcga tcaccaagaa cacgaatttg
     8701 ccaaaggctc aatctatcac ttgcttgagc ctgatattaa tcaagatatt tacggcttgc
     8761 cacaatatct ttcggccctg caatctgcgt ggttaaatga gagtgctact ctgtttcgcc
     8821 gtaagtattt tttaaatgga gctcacgccg gctttgtgtt ttatatgagc gatgcgtcac
     8881 aaaaacaaga agatgttgaa aatattcgcc agcagctcaa gcagtcaaaa ggggtgggga
     8941 attttaaaaa cttgttcgtc cacgcaccaa acggcaagaa agatggaata cagattattc
     9001 cgattgctga tgtgtcggca aaagatgagt tttttaatat caagaacgtg agccgtgatg
     9061 atgtgttagc tgctcaccgt gtgccaccac aactgatggg gattatccca aataacaccg
     9121 gcggttttgg caacgtggct gatgctgccg aggtgttttt tatcaccgag attgagccat
     9181 tgcaagagcg attgaaagag tttaatcagt ggttaggtca agaggttatc aaatttaaac
     9241 cgtctaaatt gttacagcgg acacaataaa acagatcttt acaaactgac aatacagctc
     9301 ctagattggg gcttttttaa tatataaagc aaaagttttg cctgattaga ttatatattt
     9361 cggcaattta ttatatcaaa acaacaaata aaatacactt aaaaaataat ttttccttta
     9421 tttttcaacg acttcaccac ctccaacccc gcgcggtcta tccctcacct cgcccgcaca
     9481 cttaatttgt cgatttatac gcaaatgcaa aaaggcgatc cctacaagcc ccatataatc
     9541 gcaaagcgat cttaaaaact cgatcttttt acgcaatttt acgcacctca acgcaaaaat
     9601 attgcttgtc aggaaaaatc gt
//
LOCUS       DANLXS010000090         9368 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.090, whole genome shotgun sequence.
ACCESSION   DANLXS010000090 DANLXS010000000
VERSION     DANLXS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.090"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            95..310
                     /locus_tag="PXD71_001604"
     CDS             95..310
                     /locus_tag="PXD71_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDL6291710.1"
                     /translation="MFPEFRDLITQLKQNNSHFARLFDKHNELDQRIKNMEANIELAT
                     HEEVEALKKEKLHLKDQLYAILKQESA"
     gene            complement(369..1526)
                     /locus_tag="PXD71_001605"
     CDS             complement(369..1526)
                     /locus_tag="PXD71_001605"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL6291711.1"
                     /translation="MSLDLADIRTQITNLDRNLLKLLAERHRLAFDVVRSKEITQKPL
                     RDLEREKILLQELVNFAEAENYQLDPQYVTQIFQRIIEDSVLTQQNYLQNKLNAQKEE
                     QVSIAFLGMRGSYSNMASRQFAKKYQGTLAELSCHSFNEVFEKVSLGEADFGVLPLEN
                     TTSGSINEVYDLLQHTDLTLVGELAYPIKHCVLANGTVDLAEVDTIYTHPQPAQQCSQ
                     FLAGLDKVHIKYCESSSHAMQMVARLNKPNIVALGNEDGGKLYGLTNIKTDIANQAHN
                     ITRFIVVAKEAVQVSPQVQTKTLLLMTTTQQAGALVDALMVFKNHHIRMMKLESRPIY
                     GKPWEEMFYVELEANIHSENTQKALAELEQVTSYLKVLGCYPSEIIEPVKL"
     gene            complement(1648..3714)
                     /gene="prlC"
                     /locus_tag="PXD71_001606"
     CDS             complement(1648..3714)
                     /gene="prlC"
                     /locus_tag="PXD71_001606"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDL6291712.1"
                     /translation="MSNPLLNYTGLPAFSQIKPEHIKPAVEAVIKECRETVEKVAQIE
                     NPTWENFYMPQAMAEDKFSRAWSPVGHLNAVKNSPELREAYQACLPLLSEYSTWAGQH
                     QGLYQGYVKLKNSPEFETYSVAQKKAIENSLQDFELSGISLPAEKQKRYGEISARLSE
                     LNSQFSNNVLDATMGWDIVITDEAQLKGLPESALEAAKLSAESKGKKGYRFTLEIPSY
                     LPVMTYCENKALREEMYRAYVTRASDQGPNAGKWDNSAVIDEILKLRHEKAQLLGFKT
                     YSDYSLATKMAENPQQVLDFLNDLAARSKAQGKNELCDLKKFAKAHFGIEHLDLWDIP
                     FYSEKQKQALYSVNDEELRPYFPENRVLSGLFEVIKRIFGMRVEEQKDVDVWHKDVRF
                     FNIFDKNDRLRGSFYLDLYARENKRGGAWMDDCINQKRLADGTLQKPVAYLTCNFNKP
                     IGDKPALFTHDEVTTLFHEFGHGIHHMLTEIDVGDVAGINGVPWDAVELPSQFLENWC
                     WEEEALAFISGHYETGEPLPKAKLEQLLAAKNFQAAMFVLRQLEFGLFDFRLHTEYDP
                     SKQGQVLALLKEVKDQVAVAKGVEWARTPHSFSHIFAGGYAAGYYSYLWAEVLSADAY
                     SRFEEEGIFNPETGQSFLDNILTRGGSEEPMVLFERFRGRKPTLDALLRHKGIVGNAE
                     PVDCTQ"
     gene            complement(3845..4585)
                     /gene="pflA"
                     /locus_tag="PXD71_001607"
     CDS             complement(3845..4585)
                     /gene="pflA"
                     /locus_tag="PXD71_001607"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDL6291713.1"
                     /translation="MSVVGRIHSFESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWD
                     LDGGKEITVEELMKEVTTYKHFMKATGGGVTASGGEAVLQMEFVRDWFRACKAEGINT
                     CLDTNGFVRHYSPVVDEMLEVTDLVLLDLKQLNDEVHQDLVGVSNKRTLDFARYLHKL
                     NKPTWVRYVVVPGYTDDDDSAHRLGQFIQGMDNIEKVELLPYHRLGAHKWETLGYKYE
                     LEGVMSPPKEDLERIQKIIESYGHTVKF"
     gene            complement(4834..5859)
                     /locus_tag="PXD71_001608"
     CDS             complement(4834..5859)
                     /locus_tag="PXD71_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL6291714.1"
                     /translation="MSKQDLIRSSVAEYLTFITATGESQVDAVYADENVWLSQKMMAV
                     LYDVDVRTISYHLKKIFSDNELAENSVIQFFWITAQDGKNYHTKHYNLSAIIAVGYKV
                     NSERAVQFRKWATEIVHSFAIKGFAMDDERLKNDGTILGKKYFEEQLARIREIRLSER
                     KFYQKITDIYATAVDYDRTATATKRFFATVQNKLHWAIHGHTAAELIMARANAEKPNM
                     GLTNWKDAPQGKIYPFDVVVAKNYLSDNELAQLQRLVSAYLDMAEDMAERQIPMTMAD
                     WETRLNRFLAATDREILQDAGKVTAEIAKSFALSEFEKYRVKQDLTYESDFDLLVKEV
                     AAKYHAG"
     gene            complement(6094..8418)
                     /gene="pflB"
                     /locus_tag="PXD71_001609"
     CDS             complement(6094..8418)
                     /gene="pflB"
                     /locus_tag="PXD71_001609"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL6291715.1"
                     /translation="MTQLTDAQQKAWEGFAAGEWQTEVNVRDFIQKNYTPYEGDESFL
                     AEVTPATSKLWADVMEMIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQT
                     DAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKQRQFASLQARLESGEDIQATI
                     QLREEIAEQHRALGKMKEMAASYGFDISGPATNAQEAVQWTYFAYLAAVKSQNGAAMS
                     FGRVSSFLDIYIERDLKAGKITEQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMW
                     ATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEQLPEAFKRYCAKVS
                     IDTSSVQYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGG
                     VDEKSGDQVGPKTEAITSEYLDYDDVMTRLDSFMDWLATQYVTALNIIHFMHDKYAYE
                     AALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPIRTDIEIKNKAGEVVGVAK
                     NVATDFEIEGEYPQFGNNDNRVDDIAVDLVERFMKKIQKLKTYRNATPTQSVLTITSN
                     VVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTF
                     SIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNVLNRDTLLDAVEHPEKY
                     PQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM"
     gene            complement(8508..9332)
                     /gene="focA"
                     /locus_tag="PXD71_001610"
     CDS             complement(8508..9332)
                     /gene="focA"
                     /locus_tag="PXD71_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDL6291716.1"
                     /translation="MFSPAEMAKIAEDGAVYKATKNQLYSFLSAITAGGFIAIAFVFY
                     TTTQVGASQMPWGMAKLIGGLVFSLGVIMCVVFGAELFTSSTLTIVARASQRINWLQM
                     FKNWVVVYFGNFVGAISIVLLIWFSGQIMAAKGQWGLVILNTAQHKIHHQWFEAFCLG
                     IFCNIMVCIAVWMSYAGKSLTDKAFIMMLPIAMFVASGFEHCVANMFMIPMGIAISHF
                     ASPEFWTAINVDPTQFADLDVSHFVFKNLIPATLGNIVGGVFFIGLMQWFLYIRKH"
BASE COUNT         2647 a         2111 c         1843 g         2767 t
ORIGIN      
        1 aagttcaaaa aaatagaaag atttaacagt aaatcaacaa aaaaatgcta gtatttaatc
       61 aagtaaaatt tgcaaccact attaggagtt aaatatgttt ccagaatttc gtgatctcat
      121 aacccaatta aagcaaaaca attcacattt tgctcgctta tttgataaac ataatgagct
      181 agaccagcgt attaagaata tggaggcaaa tattgaactg gcaacacatg aagaagttga
      241 ggcactaaaa aaagaaaaat tgcatttaaa agaccaactt tatgcaattc taaaacaaga
      301 atcggcttaa cctaaagtaa aagctcagaa aatattctga gctttaattt cggtcatatt
      361 agacaatatt acagttttac cggctcaata atttcgcttg ggtagcaacc caacactttg
      421 aggtagctgg taacttgctc caattccgcc aatgcttttt gggtgttctc ggaatgaata
      481 tttgcctcca gctccacata aaacatctct tcccaaggtt tgccgtaaat cgggcgggat
      541 tccagtttca tcatacgaat atgatgattt ttgaatacca ttaacgcatc gaccaatgca
      601 cccgcctgtt gcgtggtggt cattaacagt aacgttttgg tttgtacctg tggcgaaacc
      661 tgcacagcct ctttggcaac aacaataaaa cgggtgatat tgtgagcttg attagcaata
      721 tcggttttga tattcgtcaa gccatacaat tttccgccat cttcattgcc caaggcaaca
      781 atattcggct tattcaagcg agccaccatt tgcattgcgt gcgagctgct ttcgcaatat
      841 ttgatatgta ctttatctag cccggctaaa aattggctac attgctgggc aggctgaggg
      901 tgggtgtaaa tggtatccac ctccgccaaa tcaaccgtgc cgtttgccaa tacacaatgt
      961 ttgattgggt acgccaattc acccactaag gttaaatcgg tatgctgcaa taaatcatac
     1021 acttcattga tcgaacctga agtcgtgttt tcaagcggca gtacgccaaa atcggcttca
     1081 ccaaggctga ctttctcaaa tacttcatta aaagaatggc agcttaattc cgccaatgtg
     1141 ccttgatatt ttttggcaaa ctgtcgagat gccatattag aataagagcc tcgcattccc
     1201 aaaaacgcaa tcgaaacctg ctcttctttt tgtgcattca atttattttg cagataattt
     1261 tgctgagtca gcactgaatc ttcaataata cgttggaaaa tttgggtcac atattgcgga
     1321 tcaagctggt aattctccgc ttcggcaaaa tttaccagtt cttgaagcaa aatcttttcc
     1381 cgttctaaat ctctcaaggg tttttgtgtg atttctttgc tccgcaccac atcaaaggct
     1441 aagcggtggc gttctgccag tagctttaat aaattgcggt ctaagttggt tatttgggtt
     1501 cgaatatcgg ctaaatctaa agacattatt tcactctctg tcaggctaaa tgctgaaaat
     1561 tataacgagt tgcaaaagaa agaaaagaca agcggtcaaa tttacaaaaa aatttgcaaa
     1621 tctaaccgct tgtaaaacgt gtttacttta ttgagtgcaa tcaacaggct cggcattacc
     1681 tacaatgcct ttgtgtctga gcaaggcatc aagggttggc ttacgcccac ggaagcgttc
     1741 gaacagcacc attggctctt ctgaaccgcc tcgggttaag atattatcta agaaagactg
     1801 ccccgtttca gggttgaaaa tcccctcttc ctcaaagcgt gaataggcat ctgctgaaag
     1861 cacttccgcc cataaataac tgtagtagcc cgccgcatag ccgcctgcaa aaatatgcga
     1921 gaaactgtgt ggcgttctcg cccactctac gcctttggca acagcgactt ggtctttcac
     1981 ctctttaagt aaggctaaca cttgtccttg tttgctcggg tcatattcgg tgtgtaggcg
     2041 gaaatcaaac aagccaaatt ctaattggcg aagcacaaac attgccgctt ggaagttttt
     2101 cgccgccaat aattgctcta atttcgcttt tggtaacggc tcgccggttt cataatgccc
     2161 tgaaataaag gctaatgcct cttcttccca gcaccagttc tctaagaatt ggcttggcag
     2221 ctccactgcg tcccacggca caccgttaat gccggcgaca tcgcccacgt caatttcagt
     2281 tagcatatgg tgaatgccgt gtccaaactc gtggaacaaa gttgtcactt catcgtgggt
     2341 aaacaatgcc ggtttatcgc caatcggttt attgaagtta caggttaaat atgccaccgg
     2401 tttttgtaat gtgccgtcag ccaagcgttt ttggttgatg caatcatcca tccacgcccc
     2461 accacgtttg ttttcacgag cgtataaatc taagtagaac gagccacgca agcggtcatt
     2521 tttatcaaaa atattgaaaa aacgtacatc tttgtgccat acatccacat ctttttgttc
     2581 ttccacacgc ataccgaaaa tgcgtttaat tacttcaaat aagcctgata atacgcgatt
     2641 ttccgggaaa tacgggcgaa gttcttcatc attgaccgaa tacaatgcct gtttttgttt
     2701 ttcgctgtag aacggaatat cccataaatc caaatgttca atgccgaaat gggctttagc
     2761 aaattttttc aaatcgcata attcattttt gccttgagct ttcgagcgag cggctaaatc
     2821 gttcaagaaa tctaatacct gttgtggatt ttctgccatt ttggtggcta atgaataatc
     2881 agaataagtc ttaaagccga gaagttgggc tttttcgtga cgcagtttta agatctcatc
     2941 aatcaccgca ctgttatccc atttgcctgc attcgggcct tgatcagaag cacgagtcac
     3001 ataagcacgg tacatttcct cacgcagtgc tttgttttca caatacgtca tcaccggcaa
     3061 atagcttggg atttcaagag tgaagcgata gcctttctta cctttgcttt ctgcggataa
     3121 ttttgccgct tctaatgccg attccggcaa gcctttaagc tgtgcttcat cggtaattac
     3181 aatatcccaa cccattgttg catcaagtac gttgttgcta aattgcgagt tgagttccga
     3241 caagcgtgca gaaatttccc cgtagcgttt ttgtttctct gccggcagtg aaatgcctga
     3301 taattcaaaa tcttgtaagc tgttttcaat cgcctttttc tgtgcaaccg aataagtttc
     3361 aaactcaggg ctgtttttta acttcacata gccttgatat aacccttgat gttgccccgc
     3421 ccaagtgctg tattccgata ataacggcaa gcacgcttgg taagcctcac gcaactctgg
     3481 ggaatttttc accgcattca aatgccctac cggtgaccac gcacgggaga atttatcctc
     3541 tgccattgct tgtggcatat agaaattttc ccacgtcggg ttttcaattt gtgcgacttt
     3601 ttcaacagtt tcacggcatt ctttgatcac cgcttctacc gccggtttga tatgttcagg
     3661 tttgatttgt gagaacgcag gcaagcctgt gtaatttaaa agagggttag acattatttt
     3721 ttccttttat ggtcaaaatg aaaattcgta tcaagataat acacaaagtg agagagaaaa
     3781 gaaaacaagc ggtcatattt caataatttt ttgcaaaaaa atcacaaaat atgaccgctt
     3841 gtctttagaa tttgactgta tgcccgtaac tttcaatgat tttttggata cgctctaaat
     3901 cctctttagg cggtgacatt accccttcca gctcatattt atagcctaag gtttcccatt
     3961 tgtgagcccc taaacgatga taaggcagaa gttccacttt ctcaatgtta tccattcctt
     4021 gaataaactg ccctaaacga tgggcagaat catcgtcatc agtataaccc ggcacgacca
     4081 catagcgaac ccaagtcggt ttatttaatt tgtgcagata acgggcgaaa tcaagggtac
     4141 gcttattcga caccccgact aaatcttggt gaacttcatc attgagctgt tttaaatcca
     4201 gcaagaccaa atcggtcact tcaagcattt catccaccac cgggctataa tggcgaacaa
     4261 aaccattggt atctaagcag gtattaatgc cttcagcctt acaggcacga aaccaatccc
     4321 gcacaaactc catttgcaac accgcttccc cacccgaagc tgttacacca ccgccggttg
     4381 ctttcataaa atgcttataa gtcgtgactt ctttcatcag ctcttcaacc gtaatttcct
     4441 taccaccatc aagatcccaa gtatcacgat tatggcaata tttacaccgc atcaaacagc
     4501 cttgcaagaa cagaataaaa cgaatccccg gtccatctac cgtaccgcag gattcaaaag
     4561 aatggatacg tccaacaaca gacatttgtc acctcaaccc tcaaaaaatt ttgagaaatt
     4621 gtagcaaatt tttatgttgt tcgcagaaat aaatcacacc ggtatatttt aagaaaggag
     4681 aaagtcatac tattttttgc ctatttcagg aataaacttt tattaatcta ttgactccat
     4741 caacatatca accaaatatg tttaaatttt tgcatttttt aaagctattt tgaccgctta
     4801 caagcagtcg gtttttctga aaactttgca aacctagccg gcgtggtatt tggcggcgac
     4861 ctcttttacc agtaagtcga aatcgctttc gtaggtgaga tcctgcttga ctcggtattt
     4921 ttcaaattcg ctgagggcga aagatttagc aatttcagcg gtaactttgc cggcatcttg
     4981 taagatttct cggtcggtgg cggcaagaaa gcggttaagg cgggtttccc agtctgccat
     5041 tgtcatcgga atttggcgtt ctgccatatc ttccgccata tccaaatagg cagagaccag
     5101 ccgttgtagt tgtgctaatt cattatccga taaataattt ttggcaacca ccacatcaaa
     5161 ggggtaaatt ttgccttgcg gagcgtcttt ccaattcgtt agccccatat tcggcttttc
     5221 agcgttggct cgtgccataa tcagctcggc ggcggtatgc ccgtgaattg cccaatggag
     5281 cttgttctgc acggtggcaa aaaagcgttt ggtggcggtt gcggtgcggt cgtagtccac
     5341 ggcggtagcg taaatgtcgg tgattttttg gtagaacttg cgttcggata accgaatttc
     5401 ccgaatacga gcaagctgtt cttcaaagta ttttttgccc aaaatcgtgc cgtcattttt
     5461 cagccgttca tcgtccatcg caaagccttt aatcgcaaag ctatgcacga tttcggttgc
     5521 ccatttgcga aactgcaccg ctcgctcgga attgacttta taacccaccg caataattgc
     5581 cgacagatta taatgcttgg tgtggtagtt tttgccgtct tgggcagtta tccaaaaaaa
     5641 ttggataact gaattttcag ctaactcatt gtcactaaag atttttttga gatgatagct
     5701 aatggttcgt acatcaacat catacaacac cgccatcatt ttttggctaa gccatacatt
     5761 ttcatcggcg taaacggcat caacttggct ttcgccggta gctgtaataa aagtgaggta
     5821 ttccgccaca gaggaacgaa ttaaatcttg cttagacatc tctttctccc atatagaaca
     5881 ctgtaattat aaacaatatt acgaaagctg tgtaaatgaa tagccaattt ttcgagcgag
     5941 atcacaaaaa aaccgcttgc ggcataaatg ctacaagcgg ttgattatta ctcacaaaat
     6001 tgtttccaat tttgttcgct cctacggagc cgtcggcaaa gccaacgttc aaaacgcaat
     6061 gcgttttgtg gctaaaagtt ttgcaaattg taattacata ctttcagtga aggtgcgggt
     6121 aattacgtcc atttgttgct ctttggttaa agagttgaaa cgtaccgcat agcctgaaac
     6181 acgaatcgtt aattgcggat atttttcagg gtgttctacc gcatctaata atgtatcacg
     6241 gtttaacacg ttcacattta agtgctgacc gccttctaca gttgcttcgt ggtggaagta
     6301 gccgtccatt aagcctgcaa ggttacgttt ttgggcttcg taatctttac ctaacgcatt
     6361 tggtacgata gagaaagtat aagaaatacc atctttcgca taggcaaacg gtaatttcgc
     6421 cacagaagtt aatgaagcta ccgcaccttt ctggtcacga ccgtgcattg ggttcgcacc
     6481 cggtccgaat ggagcacctg agcgacgacc gtccggtgta ttaccggttt tcttaccata
     6541 taccacgtta gaagtgatag taagcacaga ctgagtcggc gttgcattgc ggtatgtttt
     6601 gagtttttga attttcttca taaaacgttc aactaaatca actgcaatgt cgtccacacg
     6661 gttatcgttg ttaccgaatt gcggatattc accctcaatt tcaaagtcgg ttgctacgtt
     6721 tttcgctaca ccaaccactt cgccagcttt gtttttgatt tcaatatcgg tacggattgg
     6781 ttttactttc gcatatttaa tggctgaaag tgagtctgcc gctactgaaa gccctgcgat
     6841 accacacgcc attgtgcgga acacatcacg gtcgtgaaga gccattaatg ccgcttcgta
     6901 tgcatattta tcgtgcataa agtggataat gttcagtgct gtcacatatt gggttgcaag
     6961 ccaatccata aagctgtcta agcgggtcat tacatcatcg taatctaagt attcacttgt
     7021 aattgcttca gttttaggcc ctacttggtc gcctgatttc tcatcaacac cgccattgat
     7081 tgcgtataac aaggttttcg ctaagtttgc acgagcgccg aagaactgca tcattttacc
     7141 cacgatcatt ggcgatacgc agcacgcaat cgcatagtca tcgttttgga aatccggacg
     7201 cattaagtca tcgttttcat attgaactga tgaagtatca atagacactt tcgcacagta
     7261 gcgtttaaat gcttccggta attgttcaga ccaaagaata gttaagtttg gctccggtga
     7321 cggccccatt gtgtaaaggg tgtgtaaaat acggaagctg tttttggtta ctaaggtacg
     7381 accgtctaaa cccataccgg ctaaggtttc ggttgcccac attgggtcgc ctgagaataa
     7441 ttgatcgtat tccggagtac gcaagaaacg caccatacgc aatttcatta ctaaatggtc
     7501 gattaactct tgggcttctt gctcggtaat tttgccggct tttaagtcac gttcgatata
     7561 aatatctaag aatgaagaca cacgaccgaa agacatcgcc gcaccgtttt gcgatttaac
     7621 cgctgcaagg taagcgaaat atgtccattg caccgcttct tgtgcattgg ttgcaggacc
     7681 tgaaatatca aaaccgtagc ttgcagccat ctctttcatt ttgcctaaag cacggtgttg
     7741 ctcggcaatt tcttcacgca gttgaatcgt ggcttgaata tcttcgccgg attctaaacg
     7801 agcttgtaat gaggcaaact ggcgttgttt gtctttcatt aagaagtctg caccataaag
     7861 tgccatacgg cggtagtcac cgataatacg accacgacca taagcatccg gcaaaccggt
     7921 aattacacct gatttacggc aacgtaaaat atccggagtg taaacatcga ataccccttg
     7981 gttatgggtt ttacggtatt cggtaaagat ttgttctact tcaggtttaa gctcacggcg
     8041 ataaactttg cacgaacctt tcaccatatt gataccacca aacggcataa tcgcacgttt
     8101 taatggagca tctgtttgca gaccaacgat tttttctaac gatttatcaa tataacccgg
     8161 tgcgtgtgag gtaatggttg atggcgtgtc gcagtcaata tcatacggtt cgtgtgtttt
     8221 gttttctact ttgatcattt ccatcacatc tgcccacaat ttgctggttg caggtgttac
     8281 ttcagctagg aaagactcat caccctcata aggtgtgtag tttttttgga taaaatcacg
     8341 tacatttact tcagtttgcc actcgcctgc ggcaaaacct tcccacgctt tttgttgagc
     8401 gtctgttagt tgagtcattt taagtcctct gtgttagtaa gttaaaacgg gaaagcggtg
     8461 attttttgca aaaaatttgc aaaatttgac cgcttgaatc tgaaatctta atgctttcta
     8521 atgtataaga accactgcat taaaccaatg aaaaacacgc caccgacaat attacccagc
     8581 gttgccggaa ttaagttctt aaacacaaaa tgagatacat ctaaatcggc aaattgcgtt
     8641 gggtcaacat taattgctgt ccaaaattca gggctggcaa agtgcgaaat cgcaatcccc
     8701 atcggaatca taaacatatt tgccacacag tgttcaaagc cggaagccac aaacattgca
     8761 atcggtaaca tcataataaa ggctttatcg gttaggcttt tacctgcata agacatccac
     8821 accgcaatgc acaccataat gttacagaaa atgcctaaac agaaagcctc aaaccattgg
     8881 tggtgaattt tgtgctgtgc ggtatttaaa ataaccaaac cccattgccc tttggctgcc
     8941 ataatctgac cactaaacca aatcaacaat acaatggaaa tcgccccaac aaagttaccg
     9001 aaatacacca ctacccagtt tttaaacatc tgtaaccagt taattctttg gctggctctg
     9061 gctacaatcg taagtgttga tgaggtaaaa agttctgccc caaacaccac acacataatc
     9121 acgccgagtg aaaataccaa accaccaatc agtttagcca taccccaagg catttggctc
     9181 gcacccactt gggttgtggt ataaaataca aatgctattg caataaaacc gcccgcagta
     9241 atggcagaga gaaaggaata taattgattt tttgttgctt tataaaccgc accatcttct
     9301 gcgatttttg ccatttcagc aggagaaaac atcagacaac cttaacaatg agaattgaaa
     9361 aaccgata
//
LOCUS       DANLXS010000091         9364 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.091, whole genome shotgun sequence.
ACCESSION   DANLXS010000091 DANLXS010000000
VERSION     DANLXS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9364
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.091"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1287)
                     /locus_tag="PXD71_001611"
     CDS             complement(<1..1287)
                     /locus_tag="PXD71_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL6291717.1"
                     /translation="MDSIPLSSLFITLAILLVLSAFFSGSETGLMSLNRYKMRHLAEQ
                     GHKGAKLAEKLLARTDVLLSLILICNNLVNIAASAIATMIGMQLSGEAGVAIATGLLT
                     FVMLVFSEILPKTVAAIYPEKVGFFASFVLTPLKKLLSPIVFLMNLIIAGLMKLLRIQ
                     QKDKQGLSSEELRSVVLDAGKFIPAEHREMLVSILDMEKVTVDDIMVPRNDISGIDID
                     DDWKAIMRQLNHAPHARVIIYKGDMDNNIVGILRVREAFRLMLEKNEFTKEMLLRATD
                     EVYFIPEGTPLTTQLMNFKANKERIGLVVDEYGDIKGLVTLEDILEEIVGEFTTSSAP
                     TLEQEVQQQSDGTVIIEGSANLRDLNKLFDWDLPLEEARTFNGLILEHLEKIPDEGME
                     FELYNLKVMILEVSENMVKQAKVEHLSPKIEKEQDSA"
     gene            complement(1411..2208)
                     /gene="ccsA"
                     /locus_tag="PXD71_001612"
     CDS             complement(1411..2208)
                     /gene="ccsA"
                     /locus_tag="PXD71_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDL6291718.1"
                     /translation="MLLPLLSIVAYALALLWVAPTLVNLENAATVNKKPNMMLVFGFG
                     LLATILHFSVLYNELFLIEGQNFTVTNVISLMSFIMAACVTLALLKWKTVWFPLIIVY
                     SFAICSVGVAGFVKGSFIRNVQENAGLLFHLGIALFSYALFFLALIYAFQLKWLDRKL
                     KSKTMFFCQILPPLMTVERHFFTLTLAAQAMLTLTLFSGVVYLHNFFEPEHIHKAIFS
                     FLAWLVYSIQLLGQWKLHWRGKRVLIYSISGMMLLTIGYFGSRLIVN"
     gene            2424..2948
                     /gene="yceD"
                     /locus_tag="PXD71_001613"
     CDS             2424..2948
                     /gene="yceD"
                     /locus_tag="PXD71_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDL6291719.1"
                     /translation="MQKVKLPLTIDPYKDAQRRVDYEGYISGKLLTRLGESVCNVQSD
                     AQVTLSLYVDPQRLTVIKGTAKVDVEFDCQRCGDPFTQTLDCSFVFSPVSNMDQADIL
                     PEIYEPIEVNEFGEVNLLDMIEDEFILELPLVPMHSEEHCEVSVSEQVFGELPEELVQ
                     KPNPFAVLANLKKN"
     gene            2965..3135
                     /gene="rpmF"
                     /locus_tag="PXD71_001614"
     CDS             2965..3135
                     /gene="rpmF"
                     /locus_tag="PXD71_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL6291720.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTTAAVSVDKTSGETHFRHHVTA
                     DGYYRGRKVINK"
     gene            3154..4176
                     /gene="plsX"
                     /locus_tag="PXD71_001615"
     CDS             3154..4176
                     /gene="plsX"
                     /locus_tag="PXD71_001615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL6291721.1"
                     /translation="MVDITLAVDVMGGDFGPRVTIPALEKALKQNPSLSFLLFGDEHQ
                     IAPLLQKHTLLNHPKIQIHHTEQVIEADVPFTTAIRQSKNSSMRLAIEAVKEGKAQGC
                     VSGGNTGALMGLAKLLIEPLPNIERPALTSLIPTLNGKSSVMLDLGANIEVSNHQLQQ
                     FAEMGNLFAQVMLDLVYPRLSLLNIGTEENKGTAQLQAVHQLLKNRQDLNYTGFIESD
                     KLTSYLTDVIICDGYTGNIALKALEGAAKNIISLFKKEKADSNICRNTKRYLLKAMFY
                     RYYRKLQQINPDRHNGATLLGLSKVVVKSHGGANINAFYYAIDYAVQQIQGDIPNKIL
                     EGLTQK"
     gene            complement(4273..5556)
                     /gene="hisD"
                     /locus_tag="PXD71_001616"
     CDS             complement(4273..5556)
                     /gene="hisD"
                     /locus_tag="PXD71_001616"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDL6291722.1"
                     /translation="MQTLIWNNLTETEQQQYLARPAQVVGESIKAAVNEIKANVIANG
                     DKALFELAEKFDKVKLESLVVSEQQIQQAQSRISPELKQAIQNAKANIEAFHKAQQNQ
                     EVDIETQPGVCCQVITRPINAVGLYIPGGSAPLFSTVLMLAVPAKIAGCNKIVLCSPP
                     PIADEILYTAHLCGVETIYAVGGAQAIIAMANGTQTVAKVDKIFGPGNAFVTEAKRQV
                     VQEGTAIDMPAGPSEVLVIADEFADPAFVASDLLSQAEHGADSQVILVTPSESLARAV
                     DEELEKQVAVLPRAETARKALAHSRTFIAENLQQAVEISNKYAPEHLIVQTENARSLL
                     ADLDNAGSIFLGAYSPESMGDYASGTNHVLPTYGYTKTYSSLGLADFSKRMTVQELTP
                     QGFLALAETVERMASAELLDAHRNAVSLRVAKLKG"
     gene            complement(5568..6467)
                     /gene="hisG"
                     /locus_tag="PXD71_001617"
     CDS             complement(5568..6467)
                     /gene="hisG"
                     /locus_tag="PXD71_001617"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDL6291723.1"
                     /translation="MTTQNRLRIAMQKKGRLSQECSELLKQCGVKIIWNDQRLIAYSE
                     NMPIEILRVRDDDIPGLVFDGVVDLGIIGENVLEEVELGRLSAGENVAYKKLRQLDFG
                     GCRLSLAIDRDKNYNNIKDLEDAVIATSYPNLLKRYMKEQNVPFKSILLTGSVEVAPS
                     AGIANAICDLVSSGATLEANGLKEVEVIYRSTACLIQRQEPLSAEKQALVDKLLTRIQ
                     GVQQANESKYIMLHAPKDKLAEITALLPGVENPTILPLANDQTRVAMHVVSQENLFWE
                     TMEQLKEMGASSVLVLPIEKMLA"
     misc_feature    complement(6500..6627)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            6826..7683
                     /locus_tag="PXD71_001618"
     CDS             6826..7683
                     /locus_tag="PXD71_001618"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL6291724.1"
                     /translation="MQISLKKIYSGKVRDLYEIDEKRMLMVATDRLSAFDVILDDPIP
                     RKGEILTQISNFWFNKLAHIMPNHFTGDSVFDVLPQEEAEAIQHRAVVCKRLIPVKIE
                     SIVRGYLTGSGLKDYQQTGTICGLTLPEGLVEASKLPQPIFTPSSKAEVGDHDINISY
                     EECERQIGQELAAKVRDAALKLYTEAANYALTKGIIICDTKFEFGLDENGELTLMDEV
                     LTPDSSRFWSVETYREGTNPPSFDKQFVRDWLENSGWNKQPPAPKIPAEVIEKTVAKY
                     QEALDLLTK"
     gene            complement(7824..9305)
                     /gene="ilvC"
                     /locus_tag="PXD71_001619"
     CDS             complement(7824..9305)
                     /gene="ilvC"
                     /locus_tag="PXD71_001619"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDL6291725.1"
                     /translation="MANYFNTLNLRQKLDQLGRCRFMDRDEFADGCNFLKGKKIVIVG
                     CGAQGLNQGLNMRDSGLDIAYALRAEAIAEKRASFKRASENGFVVGTYEQLIPEADLV
                     INLTPDKQHSKVVADVMPLMKQGAAFGYSHGFNIVEEGEQIRPDLTVVMTAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGLAIAKAWASATGGDRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSIVCYDKLVSEGKDPAYAGKLIQYGWETITEALKQGGITLMMDR
                     LSNSAKLRAFELSEQIKEELAFLFEKHMDDIISGEFSSTMMADWANGDANLLKWREET
                     GKTAFENAPQADGIKISEQEYFDNGVLMVAMVKAGVELAFDTMVSAGIYEESAYYESL
                     HELPLIANTIARKRLYEMNVVISDTAEYGNYLFSHVATPILAEKIIPTLEKGDLGEPT
                     PAVEIDNVTLRDVNDAIRNHPIELIGQELRGYMTDMKRISSQG"
BASE COUNT         2778 a         2130 c         1773 g         2683 t
ORIGIN      
        1 ggctgaatct tgctcttttt caattttcgg tgaaagatgt tcaactttcg cttgtttaac
       61 catattttca ctcacttcaa gaatcatcac tttcaagttg tagagttcaa attccattcc
      121 ttcatcagga attttttcta aatgctctaa aatcaagccg ttaaaggttc tggcttcctc
      181 aagcggtaaa tcccaatcaa atagtttgtt cagatcacgc aggttggctg agccttcaat
      241 aatcaccgta ccatctgatt gttgctgaac ttcttgctca agggtcggtg ctgatgaagt
      301 cgtaaattca cccacaattt cttctaaaat atcttcaaga gtgactagcc ctttaatatc
      361 accatactca tcaaccacta aaccaatacg ctctttattc gctttaaagt tcattaattg
      421 tgtagtgagt ggtgtgcctt caggaataaa atagacttca tctgttgcac gcagtagcat
      481 ttctttggta aattcgtttt tctccaacat taaacgaaag gcttcacgca cccgcaaaat
      541 cccaacaata ttgttgtcca tatccccttt gtagataatc acccgagcgt gaggggcgtg
      601 attaagctga cgcataatcg ctttccagtc atcatcaata tcaatgccgc tgatgtcgtt
      661 gcgaggcacc ataatgtcat caaccgtcac tttttccata tctaaaatag agaccaacat
      721 ttcacggtgt tcggcaggaa taaatttacc ggcatcaagc actacactac gcagctcttc
      781 ggagcttaag ccttgtttat ctttttgttg aatccgcaat aatttcatca gcccggcaat
      841 aatcaaattc attaaaaata caatcgggct gagtaatttt ttaagcggtg ttagcacaaa
      901 gctggcgaaa aaaccgactt tttcagggta aattgccgct actgttttag gtaggatttc
      961 cgaaaaaacc aacattacaa aagttagcaa accggttgca attgccacac cggcttcacc
     1021 ggataactgc ataccgatca tcgttgcaat cgcagaggcg gcaatgttga ctaagttatt
     1081 acaaattaag ataaggctaa gtagcacatc ggtgcgtgcc aagagttttt ccgccagttt
     1141 cgccccttta tgcccttgct cggcaaggtg acgcattttg tagcggttta aagacatcaa
     1201 gccggtttcc gagcctgaaa aaaatgcaga gagtactaaa agaatagcta aggtaataaa
     1261 taaactactc aggggaatac tgtccaaaat tcaattccta tgtaaattaa tcataaaagg
     1321 tagcgtaaca ctaaaagcgt tgtcactacc tcactttgtc ggttcagccg acttgcaaaa
     1381 aattttgctt ttttgaccgc ttgtagatca ttaattgacg atcaaacgac taccgaaata
     1441 gccgatggtg agcaacatca tacccgaaat cgaataaata agcacccgtt ttccacgcca
     1501 atgtaatttc cactgtccaa gtaactgaat gctataaaca agccaagcta aaaaggagaa
     1561 aattgcttta tgaatatgct ccggctcgaa aaagttatgc aaataaacca caccggaaaa
     1621 taaagtgagc gtcagcatag cttgtgcagc taaggttaaa gtaaagaaat ggcgttcaac
     1681 cgtcattagc ggtggtagaa tctggcaaaa gaacattgtt ttgctcttta gttttctatc
     1741 cagccatttg agttgaaaag cataaattaa tgctaaaaag aagagagcat aagaaaataa
     1801 tgcaatccct aaatggaaaa gcagtcccgc attttcttgc acattacgga taaaactacc
     1861 ttttacaaat ccagccaccc ccacactaca aatggcgaaa gagtacacaa taattaaagg
     1921 aaaccaaacc gttttccatt ttaacaacgc aagtgtgaca caagccgcca taatgaaact
     1981 catcaaagag attacatttg ttaccgtaaa attctgccct tcgattaaaa ataattcatt
     2041 atataacaca gaaaaatgca aaatcgtggc aagtaaccca aacccaaaga ctaacatcat
     2101 attaggcttt ttattaactg ttgcggcatt ttctaaattc actagagtag gggcaaccca
     2161 aagtagcgct agagcgtaag caacaatgga taaaagagga agtaacatat tgtctcgaat
     2221 ctcgtcaaat aagaataact atcagaattt taatcaaaag taaccgtttt agctagtttt
     2281 tgctgttgct agatcaaaaa ttatcctaaa aaatgtgctt ttcgccttaa attgaaccct
     2341 accttttctc ggtaagatta tctcactgaa aacaataatt ttcctttgac taatttgctt
     2401 tctatctgta atatgcacac cctatgcaaa aggtaaaact accccttacg attgaccctt
     2461 acaaagatgc tcagcgtcgc gttgattatg aaggctacat ttcaggtaaa cttctaacac
     2521 gcttgggtga atcagtatgt aatgtgcaaa gtgatgcaca agtcactctc tcgttatatg
     2581 tcgatccaca acgcctaact gtgattaaag gcacagcaaa agtagatgtg gaatttgatt
     2641 gccaacgatg tggtgacccg tttacacaaa cgctcgactg ttcatttgtt ttcagtccgg
     2701 tgtctaacat ggatcaggcg gacatcttgc ccgaaattta tgaacctatc gaagtaaacg
     2761 agttcggtga agtaaattta ctagatatga ttgaagatga atttattctt gaattacctc
     2821 tagtcccgat gcatagtgaa gaacactgtg aagtgtccgt aagtgaacag gtatttggcg
     2881 agttgccgga agaactggta caaaaaccaa acccgttcgc agtattagct aatttaaaga
     2941 aaaactagat ctaggagaaa gcccgtggct gttcaacaaa ataagaaatc tcgttcacgt
     3001 cgtgatatgc gtcgttcaca cgatgcatta acaactgcag cagtttcagt agataaaacc
     3061 agtggtgaaa ctcactttcg ccaccacgta actgcagacg gttactaccg tggtcgtaaa
     3121 gtaatcaaca agtaattcct ttcgaggttt atcttggttg atatcactct tgcagtagat
     3181 gtgatgggcg gggactttgg tccccgcgtt actattcctg cactggaaaa agccttaaaa
     3241 caaaatccaa gcctttcttt tttattattt ggtgatgaac accaaatagc ccctttatta
     3301 caaaaacaca cactactcaa ccaccccaaa atccaaattc atcataccga acaagtgatt
     3361 gaagctgatg ttccgttcac tactgcaatc cgccaaagca aaaacagctc tatgcggtta
     3421 gcgattgagg cagtaaaaga gggcaaagca caaggttgtg ttagtggcgg taacactggt
     3481 gcgttaatgg gattggcaaa attattgatt gaaccattac caaatattga acgccctgca
     3541 ctcacttcgc taattcctac cctcaacggc aaatccagcg taatgttaga tttaggggca
     3601 aatattgaag taagcaatca ccagctacaa caatttgcag aaatgggaaa cctgtttgct
     3661 caagtaatgc ttgatttagt ttaccctcgc ctgtctttat tgaatattgg tacagaagaa
     3721 aataaaggca cggcacaact tcaggcagtg catcaactgc tgaaaaacag acaagactta
     3781 aattacaccg gctttattga aagcgataaa ttaaccagct atctcactga tgtgattatt
     3841 tgtgacggct acaccggcaa tatcgcgtta aaagcgttgg aaggggcagc taaaaatatt
     3901 atttctctct ttaaaaaaga gaaagccgat tccaatattt gccgtaacac caaacgatat
     3961 ttactgaaag cgatgttcta ccgttactac cgcaaattgc aacaaattaa ccctgaccgc
     4021 cataacggtg cgaccttgct cggcttatct aaagtagtcg tgaaaagcca cggcggggca
     4081 aatatcaatg ccttttatta cgctattgat tatgcggtgc aacaaattca aggcgacatt
     4141 ccaaataaaa tcctagaggg tttaacccaa aaataagcga actcaactgt tcgcttattt
     4201 tcatttcttg tttcacttaa agatacaagc ggtcaaattt gcaaaaaaat ttacccattc
     4261 aaccgcttgt gtttagcctt ttaattttgc cactcgtaaa ctaaccgcat tgcggtgtgc
     4321 atctagcaat tctgcacttg ccattcgttc caccgtttcg gccaaggcta gaaagccttg
     4381 tggtgtcagc tcctgcaccg tcattcgctt gctaaaatca gctaaaccta agctcgaata
     4441 ggttttagtg tagccgtaag tcggtaaaac gtggttagtg ccgctagcat aatcccccat
     4501 actttccggc gaatacgccc ctaagaaaat cgagccggca ttatctaaat ccgccaacaa
     4561 cgagcgagca ttttcagttt gcacaattaa atgctcgggt gcatatttat tactaatttc
     4621 gaccgcttgt tgcaggtttt ccgcaataaa agtgcgactg tgagccaatg ctttacgggc
     4681 agtttcggca cgaggtaaaa ccgccacctg cttttctaat tcttcatcga ctgcccttgc
     4741 caagctctca cttggtgtga ctaaaatcac ttgggaatcg gctccatgtt ctgcttgaga
     4801 gagtaaatcg ctcgccacaa atgcagggtc agcaaactca tcggcaatca ccaacacttc
     4861 cgacgggcct gccggcatat caatcgccgt gccttcttgc acaacttggc gtttcgcctc
     4921 cgtcacaaac gcattgcccg ggccgaaaat tttatccact ttcgctaccg tttgtgtgcc
     4981 atttgccatc gcaataatcg cttgagcccc cccaactgcg taaatcgttt ccaccccaca
     5041 caaatgggcg gtgtagagaa tttcatcggc aatcggcggt ggtgagcaaa gcacaatctt
     5101 gttacaaccg gcaatttttg ccggcactgc aagcattaac acggtagaga aaagcggggc
     5161 tgaaccgccc ggaatataaa gccccaccgc attaatcggg cgagtgatga cttggcaaca
     5221 cacccccggc tgggtttcaa tatccacctc ttgattttgc tgtgccttgt ggaaagcctc
     5281 gatattcgct ttcgcatttt gaatcgcttg cttaagctct ggtgaaatac ggctctgtgc
     5341 ttgctgaatc tgctgttctg atacaactaa gctctctaat tttactttat cgaacttttc
     5401 agctaattca aataatgcct tatcgccgtt ggcaattaca ttcgctttaa tttcattaac
     5461 tgccgcttta atactctcgc caaccacctg tgccggacgg gcaagatatt gctgctgctc
     5521 agtttctgtt aaattgttcc aaattagggt ttgcatattt attcctttta cgccaacatt
     5581 ttctcaatcg gtaaaaccaa tacggaactt gcgcccattt cttttagctg ctccattgtt
     5641 tcccagaaca ggttttcttg gctgacaacg tgcattgcca cacgggtttg gtcgttagct
     5701 aacggtaaaa tagtcgggtt ttccacaccg ggtaataaag cggtaatttc cgccagtttg
     5761 tctttggggg cgtgcagcat aatatactta gattcgttcg cctgttgcac accttgaatg
     5821 cgggttaata atttatccac taacgcttgt ttttccgctg aaagtggttc ttggcgttga
     5881 attaggcaag cagttgaacg gtaaatcact tcaacctctt ttaatccgtt cgcctctaat
     5941 gttgcacctg aagaaaccaa gtcacaaatc gcattcgcaa ttccggcact tggagcaact
     6001 tcaaccgaac cggtgagtaa aatactttta aacggcacat tttgctcttt catataccgc
     6061 tttaataaat tggggtaaga agtggcaatc acagcatctt ctaaatcttt gatattgtta
     6121 taatttttat cacgatcaat agctaacgat aaacggcagc caccaaaatc taactgacga
     6181 agttttttat aggcaacatt ctcaccagca cttaatcgcc ctaattccac ttcctctaat
     6241 acattttcac cgataatccc aagatcaact acgccgtcaa aaactaagcc tggaatgtcg
     6301 tcatcacgca cccgtaaaat ttcaatcggc atattttctg aataggcaat caggcgttga
     6361 tcgttccaaa tgattttcac gccacattgt ttaagcaact cactacattc ttggcttaag
     6421 cgaccttttt tctgcatcgc aattcttaat cgattttgtg ttgtcattgt tgtttccttc
     6481 ttatgtttta aattgtaaaa ataaaaaaac ctctcgaaat cacttccgag agggttaaat
     6541 ttatgctgcg tttaggcgta ctcggaagtc ttatcttccg cacataccaa atgcccgaaa
     6601 gattattcag gtaaatgatg gtggtgatga gtacgaataa aagccatttt gttgttctcc
     6661 aaactaaatt gctttaaaag atacgcttaa attttggcaa tgacaagtgt tttttgcgaa
     6721 aaaattgcta tttttcatct ctctaaaaca actatcgcaa acgtttgcgt aaagcggtat
     6781 aatttgcctg attttttgca aattcaacaa agtaggaata acactatgca aatcagcctc
     6841 aaaaagatct actccggcaa agtccgtgat ctctacgaaa tcgatgaaaa acgtatgctt
     6901 atggttgcca ccgaccgatt atctgccttt gatgttattt tagacgatcc tattcctcgt
     6961 aaaggcgaaa tcctcaccca aatctccaac ttctggttca acaaattagc ccacattatg
     7021 ccaaaccact tcaccggcga cagtgtgttt gacgtgttac cgcaagaaga ggcagaagct
     7081 attcaacatc gtgcggtggt ttgcaaacgc ttaataccgg tgaaaattga atcgattgtg
     7141 cggggttatt taaccggctc cgggctgaaa gattaccaac aaaccggcac gatctgcggc
     7201 ttaacattgc cggaaggctt ggttgaggcc agcaaattac cgcaaccgat cttcacaccg
     7261 tccagcaaag ccgaggtggg cgatcacgat attaatatca gctacgaaga gtgtgaacgc
     7321 cagatcggtc aagaacttgc cgccaaagtg cgtgatgccg ctctaaaact ctacactgaa
     7381 gcggcaaact atgccctgac caaaggaatt attatttgcg acaccaaatt cgaattcggc
     7441 ttagacgaaa acggtgaact aacgctaatg gacgaagtcc taaccccgga ttccagccgt
     7501 ttttggtcag ttgaaaccta ccgagaaggc acaaatccac cttcatttga taaacaattc
     7561 gtgcgtgact ggctggaaaa tagcggttgg aacaaacaac ccccagcccc gaaaataccg
     7621 gcagaggtaa ttgaaaaaac cgtagcgaaa tatcaagaag cattggattt attaaccaaa
     7681 taacaccaca agcggtcaaa tttacgctat tttttgcaaa acaaaaacct gtaagccaac
     7741 acttacaggt tttttagtta gtggtaaaaa gtgcggttat tctaccgcac ttttgcaaaa
     7801 ttttgtgtaa atctgaccgc ttgttagcct tgcgaagaaa tacgtttcat atctgtcata
     7861 taaccacgta attcttgacc gattaactcg attgggtggt tacggattgc gtcattcaca
     7921 tcacgtaagg taacgttgtc gatttccact gccggagttg gttcacctaa atcgcctttc
     7981 tctaacgttg ggataatttt ctccgctaaa atcggggttg caacgtgaga gaataagtag
     8041 ttaccgtatt ctgcggtatc tgaaattacc acgttcattt catataaacg cttacgagcg
     8101 attgtatttg cgattaacgg taactcgtgt agagactcgt agtaagccga ttcttcataa
     8161 ataccggctg aaaccattgt atcaaaggct aattcaaccc ctgctttcac cattgcaacc
     8221 attaacacac cgttatcaaa gtattcttgc tcagaaattt taataccgtc tgcttgtggt
     8281 gcgttttcaa aggctgtttt acctgtttct tcacgccatt ttaataagtt ggcatcgccg
     8341 tttgcccagt ctgccatcat cgttgatgag aactcaccac tgatgatgtc gtccatatgt
     8401 ttttcaaata agaacgctaa ttcttctttg atttgctcag aaagctcgaa cgcacgcaat
     8461 ttggcactgt ttgataagcg atccatcatt aaggtgatac cgccttgttt taacgcttcg
     8521 gtaatggttt cccaaccgta ttggatcaat ttacctgcgt aagccggatc tttaccttcc
     8581 gacactaatt tgtcgtagca cacgattgaa cctgcttgta acataccgca aagaatggtt
     8641 tgttcaccca ttaaatcaga tttcacttct gctacgaatg aagattctaa tacgcccgca
     8701 cggtcaccac cggtagcact tgcccacgct ttcgcaatgg ctaaaccttc gcctttaggg
     8761 tcattttccg ggtgaacggc aattaaggtt ggcacaccga aaccacgttt gtactcttca
     8821 cgcacttccg tgcccgggca tttcggagcg gtcatcacaa ccgttaaatc cggacgaatt
     8881 tgctcacctt cttccacaat gttgaaaccg tgtgaataac caaatgccgc accttgtttc
     8941 attaacggca ttacatcggc aactacttta gagtgctgtt tgtccggcgt taagttaatg
     9001 actaaatccg cttcaggaat taattgctcg taagtaccca ccacaaagcc gttttcagaa
     9061 gcacgtttaa atgaagcacg tttttcagca atcgcttcgg cacgtaaagc gtaagcaata
     9121 tctaagcctg aatcacgcat atttaaacct tggtttaaac cttgagcacc acaacccaca
     9181 atcacgattt ttttgccttt taagaaattg cagccatctg caaattcatc acgatccata
     9241 aagcggcaac gacctaactg gtctaatttt tgacgtaaat ttaaagtgtt gaaatagtta
     9301 gccatttttt aatccttatt tgaattaatg agattgatat gatgttttgt ttgcacattg
     9361 ttat
//
LOCUS       DANLXS010000092         9112 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.092, whole genome shotgun sequence.
ACCESSION   DANLXS010000092 DANLXS010000000
VERSION     DANLXS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9112
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.092"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(139..214)
                     /locus_tag="PXD71_001620"
     tRNA            complement(139..214)
                     /locus_tag="PXD71_001620"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(179..181),aa:Asn,seq:gtt)
     gene            complement(220..295)
                     /locus_tag="PXD71_001621"
     tRNA            complement(220..295)
                     /locus_tag="PXD71_001621"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Phe,seq:gaa)
     gene            complement(427..1518)
                     /gene="mltC"
                     /locus_tag="PXD71_001622"
     CDS             complement(427..1518)
                     /gene="mltC"
                     /locus_tag="PXD71_001622"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDL6291726.1"
                     /translation="MKKYAKYLPMLAIIPFLIACGSNKSTKKPKSTRVDYSKDTNGLD
                     ILAGQFSHNIDDIWGSNELLVASKKDYVKYTDKFYTRSHISFEEGVITIETLGNQNHL
                     RNSIIHTLLMGRDPKGIDLFASGDAPISSNPFLNGQVEDQFGRNITNVAIANDFATYL
                     LQNKVQTRRLKNGQTVSFVTIKMVAGHIEVRARQYLPLVRKMAKQYGIEPSLILGIME
                     VESALNPYAISYANAIGLMQVVPRTAGRDIFARKGMGGQPSREYLYNPSNNIDAGTLY
                     LTILRDEYLDGIEDPVAKRYAMISAYNSGAGAVLRVFDSDRYSAIDRINDLSSDAVYR
                     ILTTAHPSSQARNYLRKVTTAREKYRNIR"
     gene            complement(1535..1804)
                     /locus_tag="PXD71_001623"
     CDS             complement(1535..1804)
                     /locus_tag="PXD71_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDL6291727.1"
                     /translation="MARTVFCEYLKQEAEGLDFQLYPGELGKRIFDSISKQAWSEWIK
                     KQTMIVNEKKLSMMNPEHRKLLEQEMVNFLFEGKEIIIEGYKPVE"
     gene            complement(1808..2953)
                     /gene="mutY"
                     /locus_tag="PXD71_001624"
     CDS             complement(1808..2953)
                     /gene="mutY"
                     /locus_tag="PXD71_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL6291728.1"
                     /translation="MNSNSPFPLAKAKASDPFAKAVLAWFEQYGRKHLPWQQNKTLYQ
                     VWLSEVMLQQTQVATVIPYFARFMERFPTVIDLANASIDEVLHLWTGLGYYARARNLH
                     KAAIQIRDEFGGEFPTRFDDVLALTGVGRSTAGAILSSVLDAPHPILDGNVKRVLSRY
                     FAVEGWAGEKAVENRLWQLSESVTPDTQVADFNQAMMDLGAMICTRTKPKCLLCPLQE
                     NCKTNAMQAWDSFPAKKPKKTLPERQTYFLVLKSGSKVLLQKREAKGIWGGLFVFPQF
                     ESLEALKRSDFMQNLQISQQLTAFRHTFSHFHLDITPVLVETDLKKTSENSPLVVEEK
                     AGNYLSKVSSNADYWYDLHQHNEVGLATPVKRILDELAFSVKNEIKD"
     gene            3037..3429
                     /locus_tag="PXD71_001625"
     CDS             3037..3429
                     /locus_tag="PXD71_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL6291729.1"
                     /translation="MKSPILGFHHIALIVSDYEKSKHFYTRILGAEIIAETYREQRAS
                     YKLDLRFKDGSQIELFSFPETPPRFTSPETCGLRHLAFRVENIEYAIAFLEQHQLPHE
                     GIRIDEITGKRFTFFRDPDDLPLEFYEV"
     gene            3430..4299
                     /locus_tag="PXD71_001626"
     CDS             3430..4299
                     /locus_tag="PXD71_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL6291730.1"
                     /translation="MLYLLIATLIWASSFIVGKVAISAFDPVQIMQIRLTLAAFIALP
                     FFIPAYKKIQKNQHIKVWLISFLTFPLYVILQFSGLKLTSAASAVALLGLNPLLTVLV
                     GYFCFKKQATRLDFILSLFACLGILIMASGSDDSGSINVLGLLLVTLGSLSFIVGMYL
                     SKEILQSVKVIDLTKIMLVQGAITALPITLLFAENWQLPTSLSAWASVAYLGIICTSL
                     AILLWNKGISQSSPILAGILLALEPVFGLLMALVFLSEQLSLVTWVGIIIVICTALLS
                     VLLPMMKRYKRLI"
     gene            4315..5028
                     /gene="sfsA"
                     /locus_tag="PXD71_001627"
     CDS             4315..5028
                     /gene="sfsA"
                     /locus_tag="PXD71_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL6291731.1"
                     /translation="MKFPPLSHGILLRRYKRFLADIQLPNGEQITLHCPNTGAMTGCA
                     NPGDTVWFSTSHNPKRKYAHTWELTQTQAGDFICVNTLRANQLVQEALENQWISELAD
                     YQHILPEQKYGSENSRIDFLLKSDLLPDCFVEVKSTTLLTENGVGMFPDAKTERGQKH
                     LRELTKIAEQGLQAVIFFAILHTGIERFEVAKQIDPKYAELFAQAIAQKVQVLSYKMA
                     ISLENGQPIAATLTKKCEF"
     gene            5089..5610
                     /gene="hslV"
                     /locus_tag="PXD71_001628"
     CDS             5089..5610
                     /gene="hslV"
                     /locus_tag="PXD71_001628"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDL6291732.1"
                     /translation="MTTIVCVRKDGKVAIGGDGQATLGNCIEKGTVRKVRRLYKDKVV
                     TGFAGSTADAFILRDLFEKKLELHQGHLVKSAVELAKEWRTERALRRLEAMMIVANDS
                     EFLLVSGSGDVIEPEQDVLAIGSGGNYAKAAALALLRTENNLSAKEIVAEALKIAGDI
                     DIYSNYNHVIEEV"
     gene            5867..7192
                     /gene="hslU"
                     /locus_tag="PXD71_001629"
     CDS             5867..7192
                     /gene="hslU"
                     /locus_tag="PXD71_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDL6291733.1"
                     /translation="MMSMTPREIVSELDAHIIGQKDAKRAVAIALRNRWRRMQLPEDL
                     RQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LADVSMKLVRQQAVEKNKMRAQDAAEDRILDVLLPPAKDQWGNVQESDNNSTRQTFRK
                     KLREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPSKTKKRKMKIKD
                     ALKVMLDEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQ
                     RDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQGRLPIRVELKSLT
                     KEDFERILTEPNASLTLQYRELMKTEGVEIEFTKDGISRIAESAFRVNEKTENIGARR
                     LHTVLERLMDGISFDASERRGEKIIIDENYVSEALNDVVENEDLSRFIL"
     gene            7268..8146
                     /locus_tag="PXD71_001630"
     CDS             7268..8146
                     /locus_tag="PXD71_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDL6291734.1"
                     /translation="MKNLYKWPAAQSLYPNQGKKSYAGKRFRYRLRSWLHHSKIKQFE
                     QFVTQNPQLIPLLNARPNYSYPVAHRFLDKRFSAKQRLQKITDNLLFLPQKLAHLPPL
                     WEQAVNFGEIIADFELWLNINEHQPMEGFWALELRHRPTNQLVYLLTFGKLDEALLIA
                     VIQGPNFEGSKELVKQLTKSCHGLRPAYLMVETMKALTAVLGYQSLLGIPQKYQNKSR
                     WVQSKRYVVDYDAIFAESGGKPGNYWQLPLELESKKIDEIPSKKRSMYRKRYEMLANL
                     KQTMELMLKTYTGLNG"
     gene            complement(8216..8932)
                     /locus_tag="PXD71_001631"
     CDS             complement(8216..8932)
                     /locus_tag="PXD71_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6291735.1"
                     /translation="MKKTLLTLLFGCVVTAQAQDIKFVMEPSYPPFEMTEEKGEIIGF
                     DVDIANAICKEMNANCTFHSQPFDSLIQSLKQKQFDAAISGMGITEPRKKQVLFSEPY
                     FPSSAAFIAKKDTDFAKVKTIGVQNGTTYQHYLAKEKKEYNVKSYASYQNAILDVQNG
                     RIDAIFGDVPVLAEMARKHEGLDFVGEKINNPNYFGDGLGIATHLKNQVLVDQFNAAL
                     KTIKENGEYQKIYDKWMGGK"
BASE COUNT         2704 a         1931 c         1838 g         2639 t
ORIGIN      
        1 ttcgggcttc gtcaaataca attctcaaaa atttagcgtt tttgaacgca taatactaaa
       61 aattgagcaa tttcttagta gttagccgga aagctcaaaa agaaaaccct gatgattact
      121 catcagggct tcagaatatg gctcctcctg cgggactcga acctgcgaca tatggattaa
      181 cagtccaccg ttctaccgac tgaactaagg aggaattgat ggtgcctcga ggcggaatcg
      241 aaccaccgac acggggattt tcaatcccct gctctaccga ctgagctatc gaggcaacgt
      301 cgttgcgtgg tgcattatgc tgatttcagc ttgcaacgtc aaacgttttt ttgcaaattt
      361 aaaaaaaccg ctgctcaaat gtgctttttt tatacttttc ggctaaaaaa cagcataatt
      421 cacacattaa cgaatattgc gatatttctc tctggcggtg gttacttttc ttaaataatt
      481 tctagcttga gaagatggat gagcagtggt taaaattcga taaaccgcat cagacgaaag
      541 atcgttaatt ctatcaatcg cagagtatcg gtctgaatca aacactcgta ataccgcacc
      601 tgctccgcta ttgtatgcag aaatcattgc atagcgttta gccacaggat cttcaattcc
      661 atctaagtat tcatcacgca aaatagtcaa ataaagcgta cctgcatcaa tgttatttga
      721 tggattataa agatattcac gacttggctg ccctcccata cctttacggg caaaaatatc
      781 acgcccggcg gtacgaggta caacctgcat taagccaatc gcattggcat agctgatggc
      841 ataagggttg agtgctgatt cgacttccat aatccctaaa atcaaacttg gctcaatacc
      901 atactgcttc gccattttac gcaccagcgg taaatattgt ctggcacgta cttcaatgtg
      961 tcctgccacc attttaatcg ttacaaagct cacagtttga ccatttttca aacggcgtgt
     1021 ttgcacctta ttttgcaaca aataagtcgc aaaatcattg gcgatggcaa cattggtaat
     1081 atttcgcccg aattgatctt caacctgtcc gtttaggaaa gggttagagc taatcggtgc
     1141 atcgcctgag gcaaataaat caatcccttt cgggtctctg cccattaata aagtatgaat
     1201 aatcgaattg cgtagatgat tctgattccc taaagtctca atcgtaatca ccccttcttc
     1261 aaacgaaata tggctacggg tgtaaaactt atccgtatat ttcacataat ctttcttact
     1321 ggcaactaac agctcgttac ttccccaaat atcatcaata ttgtgagaaa actgccctgc
     1381 caaaatatcc aaaccattgg tatctttaga ataatctact cgggtagatt ttggcttctt
     1441 agtcgattta ttgctcccac aagcaataag aaacggaata attgccaaca tcggcaaata
     1501 tttagcatat tttttcattg tgatttaacc tttattattc gaccggctta tagccttcga
     1561 tgatgatctc tttaccttca aataaaaaat tgaccatctc ttgttcgagc agtttacggt
     1621 gttcggggtt catcatactg agttttttct cattgacgat cattgtctgt tttttaatcc
     1681 actcagacca cgcttgcttg ctgattgagt caaaaatacg cttacccagc tcccccggat
     1741 aaagttgaaa atctaaacct tctgcctctt gttttaaata ttcgcaaaat acggtacgag
     1801 ccataattta atccttaatc tcatttttca cactgaatgc cagctcatct aaaatacgtt
     1861 taaccggcgt tgctaaaccc acttcgttat gttggtgcaa atcataccaa taatccgcat
     1921 ttgaagatac cttagaaaga taatttcctg ctttttcttc gacaacaagc ggtgaatttt
     1981 cacttgtttt tttcaaatcg gtttctacta aaaccggggt aatatccaaa tgaaaatggc
     2041 taaaagtatg cctaaatgca gttaattgct gtgaaatttg caaattttgc ataaaatcag
     2101 accgcttgag agcctctaag ctctcaaatt gcggaaacac aaataagccc ccccaaatgc
     2161 cttttgcttc ccgtttttgt aacagcactt tcgagccgct tttcagcact aaaaaatagg
     2221 tttgacgctc cggcaaggtt tttttcggtt tcttggcagg aaagctatcc cacgcttgca
     2281 ttgcattcgt tttacaattt tcttgtagcg ggcagagcaa gcacttaggc tttgtacggg
     2341 tgcaaatcat cgcccctaaa tccatcatcg cttggttaaa gtctgccact tgggtatcag
     2401 gcgtcacact ttcgcttaat tgccaaaggc gattttccac cgctttttca cccgcccagc
     2461 cttctactgc aaaatagcgg cttaacaccc gtttgacatt gccatctaaa atcgggtgtg
     2521 gggcatctaa cacagaagac aaaatcgccc ctgcagtact tctgcccact ccggttaatg
     2581 ccaacacatc atcaaatcgg gtaggaaatt caccgccaaa ctcatctcga atttgaattg
     2641 ccgctttatg caaatttctt gcccgagcgt aataacccag ccctgtccac aaatgcaata
     2701 cttcatcaat cgaggcatta gctaagtcaa ttacggtggg aaatcgttcc ataaaacgtg
     2761 caaaataagg aataacggtc gctacttgag tttgttgcaa cattacctcc gacaaccaaa
     2821 cctgatacag cgttttattt tgctgccaag gtaaatgttt ccgcccatat tgctcaaacc
     2881 aagctaaaac agctttggca aacggatcag aagcttttgc tttcgctaaa ggaaatgggg
     2941 aattgctgtt cattaaaaat cttgaaatcc gaaaaataaa ttatcatact gcaagcggtc
     3001 agttttacca aaaattttac acattaagga aaaaagatga aatcgcccat tttaggtttc
     3061 caccacattg cattaattgt ttccgattac gaaaaatcca aacatttcta cacccgcatt
     3121 ttaggggcag agatcatcgc agaaacctac cgagagcaac gtgccagcta taaattagat
     3181 ttacgcttta aagatggctc acaaattgaa ttattcagtt ttcctgaaac gccgcctcgt
     3241 ttcacctcgc ccgaaacctg cgggcttcgc catttggcat ttcgagtgga aaatattgaa
     3301 tatgccatcg catttttaga acaacatcaa ttaccgcatg aagggatccg aattgatgag
     3361 atcaccggca aacgcttcac gtttttccga gatccggacg atctgccgtt agagttttac
     3421 gaggtctaaa tgttatattt actgattgca accctgattt gggcgagttc attcattgta
     3481 ggtaaagtgg cgatttcagc ctttgaccct gtccaaatta tgcaaattcg gctgacgtta
     3541 gcggcgttta tcgctctgcc gttttttatt cctgcgtata aaaaaatcca gaaaaatcag
     3601 cacattaaag tctggctgat tagttttctc acttttcctc tgtacgtgat cttacaattt
     3661 tccggcttga aattaacttc cgcagcaagt gccgtggcac tactcgggct aaacccgctt
     3721 ttaactgtgt tagtcggcta tttttgtttc aaaaaacagg caaccaggct cgattttatc
     3781 ctaagtttat ttgcctgctt ggggatttta attatggcat cgggtagcga tgatagcggt
     3841 tcaatcaatg tattaggatt attgcttgta accttaggca gtttaagttt tattgtggga
     3901 atgtacctca gcaaagagat tctgcaatca gtaaaagtga tcgatttaac caagatcatg
     3961 ctggtgcaag gggcaattac cgccttacct attaccttac tctttgcaga aaattggcaa
     4021 ctgccaacca gtctctcagc atgggcgagc gtagcatatt taggcattat ttgtaccagc
     4081 ttggcaatat tgttatggaa caaaggcatc agccaatctt cgcctattct tgcaggtatt
     4141 ctactcgcac ttgaaccggt attcggactg ctaatggcat tggtcttttt atcagaacag
     4201 ctcagtttag tgacttgggt tgggattatt attgttattt gcaccgcctt actctccgtt
     4261 ttgctaccga tgatgaaaag atacaagcgg ttaatttaac aggatttttt gcaaatgaaa
     4321 tttccccctc tttcccacgg cattttactt cgccgttata aacgttttct tgccgatatt
     4381 cagttgccaa atggcgaaca aattaccctc cattgcccaa ataccggtgc gatgacaggc
     4441 tgtgccaacc ccggtgatac agtttggttt tccacctctc ataaccccaa acgtaaatat
     4501 gctcacactt gggaactaac ccaaacccaa gcgggggatt ttatttgtgt caataccttg
     4561 agagccaatc agcttgtaca agaagccttg gaaaatcaat ggattagcga gcttgcggat
     4621 tatcagcaca ttctgcctga acaaaaatat ggcagtgaaa acagtcggat tgattttttg
     4681 ctcaaatcgg accttctgcc ggattgtttt gtggaagtga aatctaccac ccttttaacc
     4741 gaaaacggtg tgggaatgtt ccccgatgcc aaaaccgagc gggggcaaaa acatttgcgg
     4801 gagctgacga aaatcgctga acagggctta caagcggtca tttttttcgc tattttgcac
     4861 acaggtattg aacggtttga agtggcaaag cagatagacc ccaaatatgc cgaattattc
     4921 gctcaagcca ttgcacaaaa agtgcaggtt ctctcctata aaatggcaat ttcattggaa
     4981 aacgggcaac ctattgcggc aaccttaaca aagaaatgtg agttttaagc ttgaaaacac
     5041 acagcatatc cccatataag ccaaagtttt taaaaagaag gatttagtat gacaacgatt
     5101 gtatgcgtgc gtaaagacgg aaaagtggct atcggtggtg atggtcaggc aacgcttgga
     5161 aattgtatcg agaaaggcac ggtgcggaaa gttcgccgtt tgtataaaga caaagtggtg
     5221 acaggctttg ccggctcgac cgctgatgcc tttattttgc gtgatttatt cgagaaaaaa
     5281 ttggagttac accaagggca tttagtgaaa tcggcagtgg aactggcaaa agagtggcgt
     5341 accgagcgtg ctttacgccg tttagaagcg atgatgattg tcgccaatga cagcgagttt
     5401 ttattggttt caggttcggg cgatgtgatt gagccggagc aagatgtgct ggcgattggt
     5461 tcaggcggta actatgcgaa agccgctgct cttgcgttat tacgcaccga aaataattta
     5521 tctgcaaaag aaatcgttgc ggaagcgtta aaaatcgccg gcgatattga tatttacagt
     5581 aactacaatc acgtgattga agaagtttag ttaaataact ctctcccctt gtgggacaaa
     5641 tgtttacatt tgaacgttgg ctttgccaac ggctccacag gagcgagcgt agcgagtatt
     5701 gagaccgaaa aatatttcgt tcagaaatat ttttcagaga gaggggttaa tgcaaaacgt
     5761 ttaagaaaaa agaccgcttg ttaccctctc cctgattttt cttctgaaca aagaaaaatc
     5821 tgtccctctc ccacaagggg agagggtaat taatttaagg aataacatta tgtcaatgac
     5881 accccgtgaa atcgtctcag aattagacgc acacattatc ggacaaaaag atgcaaaacg
     5941 tgcggtagca attgctttgc gtaaccgctg gagaagaatg cagttgccgg aagatctccg
     6001 ccaagaagtg acccccaaaa atattttaat gatcggtccg accggtgtgg gtaaaaccga
     6061 aatcgcccgc cgtttggcga agcttgccaa tgcccctttt atcaaagttg aggcaaccaa
     6121 attcactgaa gtggggtatg tgggtaaaga agtggattcg attatccgtg atctagctga
     6181 tgtgtcgatg aaattagtcc gccaacaagc ggtcgaaaaa aataaaatgc gtgcacaaga
     6241 tgcagcggaa gatcgcattt tagacgtatt attgccaccg gcgaaagatc agtggggaaa
     6301 tgtgcaggaa tccgacaata attctacccg ccaaactttc cgcaaaaaat tgcgtgaagg
     6361 gcaattagac gataaagaga ttgaaattga cgtggcagct caagtgagcg ttgagattat
     6421 gaccccgccg ggaatggaag agatgacctc gcaattacaa tcgctgtttg agggaatgtc
     6481 gccaagcaaa accaaaaaac gcaaaatgaa aatcaaagat gcgttaaagg tgatgcttga
     6541 tgaagaagcg gcgaaattag taaatcctga agagctgaaa cagcaagcga ttgaagcggt
     6601 ggaacagcac ggtattgtct ttattgatga gatcgacaaa atctgtaaaa aaggcgagca
     6661 ttcgggtgga gatgtttctc gtgaaggtgt tcagcgtgat ttacttccaa tcattgaagg
     6721 ctcaacggta aataccaagc atggtatggt gaaaaccgac catattctgt ttatctgctc
     6781 gggggcattc caagtggctc gcccgtctga tttactccca gaattacagg gtcgcttgcc
     6841 gattcgtgtg gagttaaaat cgcttaccaa agaagatttt gagcgtattt taaccgaacc
     6901 gaatgcctca ttaaccttgc aatatcgtga gttgatgaaa actgaaggcg tggagattga
     6961 gttcactaaa gacggtatca gccgtattgc agaaagtgca ttccgtgtga acgagaaaac
     7021 cgaaaacatc ggggcaagac gtttacacac cgtgcttgag cgtttgatgg acggcatttc
     7081 gttcgatgcc agcgaacgcc gtggtgaaaa aatcatcatt gatgaaaact atgtttccga
     7141 agcgttaaat gacgtggtgg aaaacgaaga tttaagtcgc tttattttgt agttttaagt
     7201 tatacaagcg gttattttag cgaaaaatct tgcgaattag ccgcttttca tattgttagt
     7261 tgtaaaaatg aaaaatctat acaaatggcc tgctgcacaa tcgctttatc caaatcaggg
     7321 aaagaagtct tacgcaggga aacgttttcg ttaccgtctt cgctcttggt tgcatcattc
     7381 caaaatcaag caatttgagc agttcgttac acaaaatcca caactgattc cgctcttaaa
     7441 tgctcgacca aattacagct accccgtggc tcaccgcttt ttagataagc gtttcagtgc
     7501 aaaacaacgc ttacaaaaaa tcacagataa cctgctgttt ttaccgcaaa aattggctca
     7561 tttaccacct ctatgggaac aagcggtcaa ttttggtgaa attattgcag attttgagtt
     7621 atggcttaac atcaatgaac atcagccgat ggaaggtttt tgggcattag aacttcgcca
     7681 cagaccgact aatcagctag tttatttatt aacatttggt aagttagatg aagccttgtt
     7741 gatagccgta attcaaggtc cgaattttga aggttcaaaa gagctggtga aacaattaac
     7801 caaaagttgc cacggcttac gcccggccta tttaatggtt gaaacgatga aggctttaac
     7861 ggctgttctt ggctatcaga gtttactagg cattccacag aaataccaga ataaatcccg
     7921 ctgggtgcaa agtaagcgct atgtagtgga ttatgatgct atttttgcgg aatcgggcgg
     7981 aaagccggga aattattggc agttacccct tgaattggaa agtaaaaaaa tagatgaaat
     8041 tccaagcaaa aaacgctcaa tgtatcgcaa gcgttatgaa atgctagcta atttaaagca
     8101 gaccatggag cttatgctta aaacttatac tggcttaaat gggtaaatca aagaatacaa
     8161 gcggtcaaaa tctgcaaaac tttgcaaaac tttgcaaaaa atgaccgctt acaagttact
     8221 tgccgcccat ccatttatca tagatttttt gatattcgcc attctcttta atcgttttta
     8281 atgctgcatt gaattgatca actaatactt ggtttttcaa gtgagttgca atgcctaaac
     8341 catcaccaaa atagtttgga ttattgattt tctcccctac aaaatctagc ccttcgtgtt
     8401 ttcttgccat ctcagccaat accggcacat cgccaaaaat agcatcaatt cgcccatttt
     8461 gcacatctaa aatagcattt tgataagagg cataagattt cacattgtac tcttttttct
     8521 cttttgctaa atagtgttgg taagtagtcc cattttgcac accaatcgtt ttcaccttag
     8581 caaaatctgt atcttttttc gcaatgaagg cagcagagct tggaaagtaa ggctcgctaa
     8641 ataatacttg tttcttacgt ggttccgtaa tacccatacc tgaaattgca gcatcaaatt
     8701 gtttttgttt taggctttgg attaagctat caaaaggttg gctatggaat gtacaatttg
     8761 cattcatctc tttacagata gcatttgcaa tatccacatc aaaaccgata atttctccct
     8821 tctcttcggt catttcaaat ggaggatagc ttggctccat cacaaatttg atatcttgtg
     8881 cctgcgcagt aaccacacac ccgaataaaa gggtcaaaag tgtttttttc ataaaaagtc
     8941 cctgtgtttt cattataagg attaccactt taacgcagtt actttcttaa aaaaagtctt
     9001 cttttcataa agtttgtttt atgtcataca aacacatcaa attgagatgt agtttctcaa
     9061 tcctcttgat tcctctatct caaaaaaaca acccaaaaga aaaaagaaaa gt
//
LOCUS       DANLXS010000093         8954 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.093, whole genome shotgun sequence.
ACCESSION   DANLXS010000093 DANLXS010000000
VERSION     DANLXS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.093"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..310
                     /locus_tag="PXD71_001632"
     CDS             <1..310
                     /locus_tag="PXD71_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291736.1"
                     /translation="KPIFGHRFSNFKDLYNTLKQIMRFRNRLYHQEVVWNKRIAKKPT
                     HALENLEKTYKQFELALQKIAPERFAFRQLSQALIWQRQIFFDQQIFEAEIVILPQHI
                     "
     gene            complement(346..942)
                     /locus_tag="PXD71_001633"
     CDS             complement(346..942)
                     /locus_tag="PXD71_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291737.1"
                     /translation="MKKLLMTLCCLPAFALANETGESCSKIEDSAKRLECYDSVFVKK
                     DTGKDEATDEKSNWEYEQKKDELRNATTYFAKIRSTNTIDFGFPYNSSSMNLMLRKDP
                     KYGNDVIFSVHGQFNGCMIESCKITVKFDDGKLESYRMIGADGGSNDTLFIENAKAMK
                     TFVDKLKKSKKLIVEASFYNYGKGQFTFDTQGLEWKHF"
     gene            1588..1773
                     /locus_tag="PXD71_001634"
     CDS             1588..1773
                     /locus_tag="PXD71_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291738.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGFWQ
                     MESNGNQYCGGKYGTKN"
     gene            1757..1954
                     /locus_tag="PXD71_001635"
     CDS             1757..1954
                     /locus_tag="PXD71_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291739.1"
                     /translation="MARKIDAGVEIVEVDKRFIAKFFYDGKLQHTTYPQYNRQNAIML
                     INRKIERFNIGRDKPIKLYKE"
     gene            1957..2931
                     /gene="ssb"
                     /locus_tag="PXD71_001636"
     CDS             1957..2931
                     /gene="ssb"
                     /locus_tag="PXD71_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL6291740.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDSVAKISVATSTEWTDKTS
                     GEKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSNSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIRVRWITKEQA
                     KQFINNISTDWMKTICSFALLTGARRTEILSMTWDKIDFIRKVAIVSNDIAKSEKARS
                     LLLNDDAIELLQKQRGKNPKYVFVGSKGQALQDINRKIFNKATEKCLLVDFHFHDLRH
                     TWASWHVQAGTPLFTLKELGGWETLEMVKKYAHLNADHLLNHANSVKF"
     gene            complement(3018..3094)
                     /locus_tag="PXD71_001637"
     tRNA            complement(3018..3094)
                     /locus_tag="PXD71_001637"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3058..3060),aa:Arg,seq:tct)
     gene            complement(3119..3195)
                     /locus_tag="PXD71_001638"
     tRNA            complement(3119..3195)
                     /locus_tag="PXD71_001638"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3159..3161),aa:Pro,seq:tgg)
     gene            3365..4225
                     /gene="folD"
                     /locus_tag="PXD71_001639"
     CDS             3365..4225
                     /gene="folD"
                     /locus_tag="PXD71_001639"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDL6291741.1"
                     /translation="MTAQIISGSEVSSQIKKKVSEKIAHYLEQGKRNPGLAVILVGND
                     PASQVYVGSKRKSCAEIGIASKSYDLPEETTEAELLALIEKLNQDDSVDGILVQLPLP
                     KQIDSTKVIEAILPHKDVDGFHPYNVGRLSQRIPTLRPCTPYGVMKLLETTGVDLYGQ
                     HAVIVGASNIVGRPMALELLLGGCTVTVTHRFTKDLEHHVRQADILVVAVGKPEFIPG
                     EWVKDGAIVIDVGINRGADGKLKGDVEYSVASQKAAFITPVPGGVGPMTVAMLMQNTL
                     QTYENHLQAV"
     gene            4339..5790
                     /gene="thiI"
                     /locus_tag="PXD71_001640"
     CDS             4339..5790
                     /gene="thiI"
                     /locus_tag="PXD71_001640"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL6291742.1"
                     /translation="MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNVLLKETENVSVV
                     RHWDFIEVRAKVDEETALVLDLLKRTPGIHHILEVEETPFTDMHDIFEQTYAAVKAEL
                     EGKTFSVRVRRKGKHDFRSLDVERYVGGGLNQRIETAKVKLTNPDVTVRIDIEHDKML
                     LIKARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEI
                     GVKQMAYHIWSRYSTSHKVRFIAINFENVVGEILEKVDNGQMGVVLKRMMVRAASRIA
                     ERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHDKEQIIAMAKAIGTD
                     DIAKSMPEFCGVISKNPTVKAIETKILEEEGNFNFEVLEQAVQTASYLDIREIAQQTE
                     KEVVSVETTTELSKNDVVLDIRSPEEVDEKPFRLDGVEVKELPFYKLSSQFSTLDQSK
                     TYLLYCQRGVMSKLQALYLKENGYSNVKVFRVK"
     gene            complement(5835..6542)
                     /locus_tag="PXD71_001641"
     CDS             complement(5835..6542)
                     /locus_tag="PXD71_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HDL6291743.1"
                     /translation="MFKEKPVISNQHGALAMAMLPFLYAVIESGFSTLHIFFGLSWLF
                     LYFFSYPFLSLFSKKPTERNKKWAKIYFGLSILFALPLIYAQPSILQFLPIILPLGLV
                     QVHYAKQKDERNLWNDIAGILTFGIMGMAAYYLVTKQYQFEILLHPTLFFIATTFYIK
                     SMVRERRNPIYMEISIAIHLVLAMLYILCSLQAVFFAYLIATARAIIVPSFGWNVKQV
                     GMLEFPVILLFLASLIW"
     gene            complement(6610..7584)
                     /gene="pfkA"
                     /locus_tag="PXD71_001642"
     CDS             complement(6610..7584)
                     /gene="pfkA"
                     /locus_tag="PXD71_001642"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL6291744.1"
                     /translation="MSKKIKKIAVLTSGGDAPGMNAAIRGVVRTALNEGLEVCGIQDG
                     YYGLYHDKVIPLDRRSVSETINRGGTFLGSARFPDFKRPEVRKHCVETLKKYSIDALV
                     VIGGDGSYMGAKLLTEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETALDAIDRLRD
                     TSSSHQRISIVEIMGRHCGDLTLSAALAGGCEYVIVPEKGLDKDALMRSIEEGFHKGK
                     RHSIIAITELMTDVHALAKEIEARFGNETRATVLGHIQRGGSPCAFDRILASRMGVYA
                     VELLLQGFGGHCVGIQNEQLVHHDIIDAINNMRRPFKEELFEASRKLF"
     gene            complement(7691..8887)
                     /locus_tag="PXD71_001643"
     CDS             complement(7691..8887)
                     /locus_tag="PXD71_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL6291745.1"
                     /translation="MLNTQFRTHFPFFEKSDGWTYLDSAATTLKPDVLIEATSEFYAS
                     AGSVHRSQYDLPQTEQYELARDLVAKRFNVETREAVIWTSGTTQSINTVAYGLEHQIA
                     AGDEIVISVAEHHANFIPWQQLAQRTRAKLVVLPLNDKYEIELETLKNALSEKTKIVA
                     FNLVSNVTGVRQPAEQIIPLIRQSCSAKIVLDIAQAVCSEQVDVQKLDADFYAFSAHK
                     MYGPNGVGVLTGKLQSLEQIQPLVFGGKMLKNITESELTVAELPYRLEAGTPNIAGII
                     GFGQVLAWLEKWDFVALNHQLYDLAEYTCKRLNSYQNLQIIGQQNTPTISFVVKNQHP
                     ADIAAFLTQSKIAIRQGEHCAKPYLRHLEEVGTVRLSLAHYNNQQDVEHFFNALDFTL
                     EILQES"
BASE COUNT         2793 a         1752 c         1861 g         2548 t
ORIGIN      
        1 aaagcctatt tttggacata gatttagtaa ttttaaggat ttatacaata cattaaagca
       61 aattatgcgt tttagaaatc ggctgtatca tcaagaagtg gtttggaata agcgaattgc
      121 caagaaacca acgcacgcac tagagaattt agaaaaaact tacaagcaat ttgagttagc
      181 actacaaaag atagcacctg aacgatttgc gtttcgtcag ctttcacaag cacttatttg
      241 gcaacgccaa atctttttcg accaacaaat ttttgaagct gaaatcgtta ttttgcccca
      301 acatatctaa gcaaaaagct acgcatctat gtagcttttt cttaattaaa aatgtttcca
      361 ctctaaccct tgtgtatcaa aggtaaattg ccctttgcca taattgtaaa aactcgcctc
      421 tacaattagt tttttcgact ttttcaactt atctacaaag gttttcattg cttttgcatt
      481 ttcaatgaaa agcgtatcat tgctgccacc atccgcccca atcattcgat aactttccaa
      541 tttaccatca tcaaacttca cagtaatttt acagctctca atcatacagc cattaaattg
      601 accgtgtacg ctaaaaatta catcattgcc atatttaggg tctttgcgta acattaaatt
      661 cattgatgat gaattatacg ggaaaccaaa atctattgtg ttagttgatc taattttagc
      721 aaagtaagtc gttgcattac gcaattcatc tttcttttgt tcatattccc aattagactt
      781 ttcatcggta gcttcatctt taccagtatc ttttttcaca aagacactat cataacattc
      841 taagcgtttt gcactatctt caatcttaga gcaactttct ccagtttcgt tagctaaagc
      901 aaaagcgggc aaacaacata aagtcatcag tagttttttc atataacctc catagaaatc
      961 ccttcttatt ttctcaaaag cgtggttttc ggtctgtgat caagctcgca atctcaataa
     1021 ttatatttta atggtttaaa aaacaatcaa ttaagaaatc ataactaatt gaaatttaag
     1081 aaatcttaaa aatacttatt aaataagctt aaattttgat tgacttgtaa tttaagtttt
     1141 cttaaactta attcatcaaa acgagctacg gctcaatgct ctttaacaat tcggtgcttg
     1201 tgcggacgat attaacaacc tcgggcagtt gtaaagtaat gctttataac tcagtaaggt
     1261 tggttagacc ctgacataca aatttgaacg acttgccaaa aagaggtggc aaggtttagg
     1321 caacacactc gcaaggtgtg gagagctgaa aaggcaacac tggtatcagt cctaagcaat
     1381 cacgcttatc tcaagtttga gtaagtacgg atacaagcag cacccgacgg aatggcgaaa
     1441 gccgggcggg cgacggcgtg ggcagacacg catttattcc aaagctcatt tcaccaagtg
     1501 tgtttcggaa tacccacaac tagacgcaag tcgctccatt tgcccaccgt aaaacgtggg
     1561 ctttttttaa ccctaaagga caatcctatg aacaaaatga aacaacttgc cccatttgcc
     1621 ttgctaattt tgctattagg cattgtcggg cgaatggatt atgacgacca catacaaatg
     1681 caacgttaca aatgcgaacg aaatcaaggc ttttggcaaa tggaaagcaa cggcaaccaa
     1741 tactgcggag ggaaatatgg cacgaaaaat tgatgccggt gttgagattg ttgaggtaga
     1801 taaacgattt atcgccaagt ttttttatga cggcaaatta cagcacacaa cctatccaca
     1861 atacaaccgc caaaacgcaa ttatgttgat taaccgcaaa attgagcggt tcaacattgg
     1921 cagagacaaa ccaatcaaac tttataagga ataacaatgg caggaattaa caaagtgatt
     1981 attgtcggtc gcctaggtaa cgacccggaa ctacgaacaa tgccaaatgg cgattcagtc
     2041 gccaaaatca gtgtagcaac ttcaaccgaa tggacggata aaacctcagg cgagaaaaaa
     2101 caagccacag agtggcatag cattatcgcc tttcggaatt tagctgacat tatcggcaaa
     2161 tacctgaaaa aaggctcgca agtgtatgtg gaaggcaagc tacgcactcg caaatggcaa
     2221 gcccaagacg gcacagaccg ctggacgacc gaaatcatcg ccgatcagct acaaatgctc
     2281 ggtagctcaa acagcggaaa cagcaataat aattgggcaa ccgaaccggc aggaaaccca
     2341 ccaccaacca atccatataa ccacgtaatg acggattcag aatcacgaga ttttgatgat
     2401 gatatccggg ttcgttggat taccaaagaa caagccaagc agtttattaa taatattagt
     2461 actgattgga tgaaaactat ctgctctttt gctttattaa caggagcaag gcgaacagaa
     2521 atactttcta tgacttggga taaaatagat tttatccgta aagtagcgat tgtttcgaat
     2581 gatattgcca aatctgaaaa agcgagatcg cttttactta atgatgatgc aattgaatta
     2641 ttacaaaagc agagagggaa aaatcctaaa tatgtttttg taggaagcaa agggcaggca
     2701 ttacaagata ttaaccgcaa aatatttaac aaagcgactg aaaaatgttt attggttgat
     2761 ttccatttcc atgatttaag gcatacttgg gccagttggc acgttcaggc cggcacacca
     2821 ttatttacct taaaagaatt gggtggctgg gaaacattag agatggtaaa gaaatatgcc
     2881 catcttaatg cagaccattt actcaaccac gctaactcag ttaaattcta gtcacaaatt
     2941 agtcacaaag tatttttgac ataggaaatt aataactgat atttaaatgt aactatttga
     3001 aatataaggg taattaatgg cacgccctaa aggattcgaa cctttgaccc acgccttaga
     3061 agggcgttgc tctatccagc tgagctaagg gcgcagatga agataatata aaaagaagtg
     3121 gtcggcgaga taggatttga acctacgacc cactggtccc aaaccagttg cgctaccaag
     3181 ctgcgctact cgccgacata agatgttgtt gattatagat ttctttgcac aacagtcaat
     3241 ctttttttgt gaatgtcggc ttaaatgatt gaagtttatc cgttattcaa aattagctta
     3301 tcaaaaaggc tattttttga gagaatgacg aatgttttgc caagtgatat ttcgggaggt
     3361 agtaatgact gcacaaatta tttcggggag tgaggtttct tcacaaatta aaaaaaaggt
     3421 gagtgagaaa atcgctcatt atcttgaaca gggtaaacgt aaccctggtc ttgcggtcat
     3481 tttagtgggg aatgacccag cctctcaagt gtatgtgggg agtaaacgca aaagctgtgc
     3541 cgaaattggg attgcgtcga aatcttatga tttacccgaa gaaaccacag aggctgaatt
     3601 attagcactg attgaaaagt tgaatcaaga tgattcggtc gatgggattt tagtccaatt
     3661 accgttacct aaacagatcg attcgactaa agtgattgaa gccatcttac cacataaaga
     3721 tgttgatgga ttccaccctt ataatgttgg gcgtttatct cagcgtattc ccactttacg
     3781 cccttgcacg ccatacggtg tgatgaagtt gcttgaaaca actggggtag atttatatgg
     3841 tcaacacgca gtgattgtag gtgcgtctaa tattgttggt cgcccgatgg cgttagaatt
     3901 gttgcttggt ggttgtacag tcacagttac tcatcgcttt accaaagatt tagagcatca
     3961 cgttcgccaa gcagatattt tagttgtggc agtcggcaag cctgaattta ttcccgggga
     4021 gtgggttaaa gacggtgcta tcgtgattga tgtagggatt aaccgtggag cagacggcaa
     4081 actaaaaggt gatgtagagt attcagttgc ctcacaaaaa gcggcattta tcacccctgt
     4141 ccccggtggg gtaggcccga tgacggtggc aatgttaatg caaaatacct tgcagacgta
     4201 tgaaaaccat ttacaagcgg tctaatttta cggatatttt gcaaatcctc ggcattggtc
     4261 gaggatttct ttttctgggc tttttcagca gaaaaggcta aaatacttgc catttttatt
     4321 acacgatcct tattaaatat gaaatttatc atcaaacttt tccctgaaat tatgattaaa
     4381 agcgactctg ttcggaaacg ctttatcaaa attttaacct caaatattcg caacgtatta
     4441 ttaaaagaga ctgaaaatgt gtctgtggtt cgccattggg actttattga agttcgggcg
     4501 aaagtggacg aagaaacggc attagtgctg gatttgctta aacgcacgcc gggtattcac
     4561 catattttag aagtggagga aacccctttt acggatatgc acgatatttt tgagcagact
     4621 tatgccgcag tgaaagctga actagaaggc aaaacgttta gcgtgcgggt tcgccgtaaa
     4681 ggaaagcacg atttccgttc tcttgatgta gagcgttatg tcggtggtgg gctgaatcag
     4741 cggattgaaa cggcgaaagt gaaattaacc aacccagatg ttacggtcag aattgatatt
     4801 gaacatgata aaatgttgct gattaaagct cgccacgaag ggttaggcgg gtatccaatc
     4861 ggcacacaag aagatgtgtt gtcattgatt tcgggtggtt ttgattcggg tgtatcgagc
     4921 tatatgctga ttcgcagagg ttctcgtgtg cattactgtt ttttcaattt aggtggtgcc
     4981 gcacacgaaa ttggcgtgaa acagatggca tatcatattt ggagccgtta tagtacctcg
     5041 cacaaagtcc gttttatcgc cattaatttt gaaaatgtag tgggtgagat tttagaaaaa
     5101 gttgataacg gacaaatggg cgtggtatta aaacgtatga tggtgcgagc ggcaagccgt
     5161 attgccgagc gttttgatat tcaagcgatt gtgaccggtg aggcgttggg gcaggtctct
     5221 agccaaacac tgactaattt acgtttaatt gataaggcat cagatacgct ggtattacgt
     5281 ccgctcatca ctcacgataa agagcagatt attgcaatgg caaaagcgat aggcactgac
     5341 gatattgcta aatcaatgcc ggagttctgt ggagtaattt ctaaaaatcc gacggttaag
     5401 gcaatcgaaa ctaaaatttt ggaagaagaa ggcaacttta attttgaggt actagaacaa
     5461 gcggttcaaa cggcgagtta tttagatatt cgtgagatcg ctcagcaaac cgaaaaagag
     5521 gttgtttcgg ttgaaaccac aacagaattg agtaaaaacg atgttgtgct agatattcgc
     5581 agcccggaag aggttgatga aaaaccattc cgtttagacg gggttgaagt aaaagaactc
     5641 ccgttttata aactttccag ccagtttagt acgcttgacc aaagcaaaac ctacttgctc
     5701 tattgccaac gtggggtaat gagtaaactg caggctcttt atttgaaaga gaatggctac
     5761 agcaatgtaa aagtgtttcg agtgaaataa tgcctctttg attgcacctt aatgattgat
     5821 taaggtgcaa ttttttacca aataaggctt gctaaaaaga gtaagatcac aggaaattca
     5881 agcattccaa cttgtttaac attccagcca aagcttggca caataatggc tcgggcagtt
     5941 gcaattaaat aagcaaaaaa gaccgcttgt agagagcaga gtatgtaaag cattgctaaa
     6001 accaaatgta ttgcaatact gatttccata taaattgggt ttcttcgctc tcgcaccata
     6061 ctctttatat agaaagtcgt agcaataaaa aatagggtag ggtggagcag aatctcaaac
     6121 tgatattgtt tcgttaccag ataataagct gccattccca ttatgccaaa ggttagaatg
     6181 ccggcaatat cattccataa attgcgttca tctttttgtt tggcgtagtg aacttgcact
     6241 aacccaagcg gtagaattat cggtaaaaat tgcaaaatcg aaggctgagc gtagatcaaa
     6301 ggtaatgcaa atagaatgct taagccaaag tagattttcg cccatttttt attgcgttca
     6361 gttggctttt tactgaaaag tgagagaaaa gggtaggaaa agaagtagag aaacagccac
     6421 gacagcccaa agaaaatatg caaagtgcta aaaccacttt caataacggc gtagagaaac
     6481 ggcaacattg ccattgctaa tgcaccgtgt tggttggata taacgggttt ttctttgaac
     6541 attcaatttc cctcttaatg tggcaaataa aaaggcgact aaacagccgc ctttaaattg
     6601 taagaaaagt tagaataatt ttctagaggc ttcaaataat tcttctttga acggacgacg
     6661 catattattg atcgcatcaa taatatcgtg gtgaaccaac tgctcgtttt gaataccgac
     6721 acagtgaccg ccaaagcctt gcagtaataa ttccaccgca tagacgccca tacgggaggc
     6781 caaaatacgg tcgaaggcac aaggcgaacc accacgttga atatgaccta atacggttgc
     6841 acgggtttca ttgccgaaac gtgcttcaat ttctttcgct aatgcgtgaa catcggtcat
     6901 caattctgta attgcaataa ttgaatggcg tttacctttg tggaaacctt cttcaatgct
     6961 tcgcattaat gcatctttat ctaagccttt ttccggtacg atcacatatt cgcaaccacc
     7021 ggcgagggca gcacttaggg ttaaatcacc acagtggcga cccataattt ccacaatcga
     7081 aatacgttgg tgtgaacttg aagtatcacg caggcggtca atcgcatcta atgcggtttc
     7141 aagggcagtt tgatagccga tggtgtaatc cgttcccaca atatcgttat caatcgtacc
     7201 cggaataccg atacaagggt aaccaaattc ttcggttaat aatttagccc ccatataaga
     7261 accgtcgcca ccaataacca ctaatgcatc aatgctgtat ttttttaagg tttcgacaca
     7321 atgtttgcga acttctggtc ttttaaaatc agggaagcgg gcagaaccta agaatgtacc
     7381 gcctcggttg atggtttcag acacggaacg gcggtcaagt gggatcactt tatcgtggta
     7441 aagcccatag tagccgtctt gaataccaca aacctctaaa ccttcattaa gtgcggtacg
     7501 aaccacacca cggattgccg cgttcattcc tggtgcatca ccaccactgg ttaatactgc
     7561 aatttttttg atttttttac tcatttaaaa acaccttgtt gataaattaa agttaaattg
     7621 gaaactggtg caaggttaca atattcagcc ttttcgttct actaaaaaat acgtattttt
     7681 ttgatttagc ttagctttct tgcaaaattt caagggtaaa atctagagcg ttgaaaaaat
     7741 gctcaacatc ttgttgatta ttgtaatggg caagtgaaag tcgaactgtt ccgacctctt
     7801 ctaaatggcg taaataaggc ttggcacaat gctccccttg gcggatcgca attttacttt
     7861 gggttaagaa agcggcaata tcggcagggt gctgattttt caccacaaag ctaatagtcg
     7921 gtgtattttg ttgcccgata atttgcaaat tttgataaga atttaaccgc ttgcaggtgt
     7981 attcagccaa gtcataaagc tgatgattaa gagcgacaaa atcccatttt tcgagccaag
     8041 ctagcacttg cccaaagccg ataatgccgg cgatattcgg cgtgccagct tccaagcgat
     8101 aaggtaactc tgccacagtt aattcacttt ccgtaatgtt tttcagcatt ttgccgccaa
     8161 atacaagcgg ttgaatttgc tccaaacttt gcaatttgcc ggtaagcacg cccacaccgt
     8221 ttggaccata cattttatgg gcggaaaaag cgtagaaatc tgcatctaat ttctgaacgt
     8281 ccacttgctc tgagcaaact gcttgagcaa tatctaacac aattttagca gagcaagatt
     8341 ggcggataag cgggattatt tgttccgctg gctggcgaac tcctgttacg ttagaaacta
     8401 aattaaaggc aacaattttg gttttttccg ataacgcatt tttaagggtt tcaagctcaa
     8461 tttcatactt atcattaagt ggcaacacca cgagtttcgc ccttgtacgc tgtgccaact
     8521 gctgccaagg aataaaatta gcgtgatgtt ccgccactga aattacaatt tcatcgcctg
     8581 ctgcaatttg atgctccaag ccataagcca cggtgttaat actttgggtt gtaccgcttg
     8641 tccaaatcac cgcttcacga gtttccacat tgaaacgttt tgcgactaaa tctcgagcta
     8701 attcatattg ttcggtttgt ggcaaatcgt actgactgcg atgcaccgac cccgcagaag
     8761 cgtaaaactc acttgttgct tcaatgagta catcaggttt cagcgttgta gcggcagaat
     8821 ctaaatacgt ccatccgtca gatttttcaa aaaaagggaa atgggtgcga aattgggtgt
     8881 taagcataag ttaattccta agagttgtgg aaaattgagt taccaagaaa atacctatta
     8941 taaaggaatg tttt
//
LOCUS       DANLXS010000094         8864 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.094, whole genome shotgun sequence.
ACCESSION   DANLXS010000094 DANLXS010000000
VERSION     DANLXS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.094"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..698
                     /gene="znuC"
                     /locus_tag="PXD71_001644"
     CDS             <1..698
                     /gene="znuC"
                     /locus_tag="PXD71_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDL6291746.1"
                     /translation="QNINLSIYPNSITTIVGPNGGGKSTLLKILLKLLSPNKGKVIHQ
                     KGLKIGYVPQKLHLEPSMPITVYRFLALKPHTTKEMINQALDLFSITHLTENSMQKLS
                     GGELQRVLLARAILDRPQLLVLDEPMQGVDIIGQTELYQLLNKTRDLLNCAILMVSHD
                     LNIVMANTDEVLCINKHICCAGSPEVITHDPSFIHFFGDQFARNVAFYSHKHNHHHNL
                     HGDICLGECSCEH"
     gene            863..3676
                     /gene="sucA"
                     /locus_tag="PXD71_001645"
     CDS             863..3676
                     /gene="sucA"
                     /locus_tag="PXD71_001645"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDL6291747.1"
                     /translation="MQYTHFDDWLASSALGGANQSYIEEIYEQYLENPANVDESWRNI
                     FDSFPKTPQVEQAHTPIRDYFRKLAREQQPQSVTIIDPEAGAKQVRLLQWINAHRFRG
                     YLEAKLDPINYYRWKTSSVPELDYKYHGFTDEDLNETFTVGRYVYDKDTMKLSELDKA
                     LKETYLGPIGLEFMHVQDIEQRNWLQAKMESVLNKPLFTKAEKLNLLSELTAADGLER
                     YLGAKFPGAKRFSLEGSDAFIPMMKEIIRHASRQGMTDVVLGMAHRGRLNMLVNVFGK
                     RPAELFDEFAGKHADESRTGDVKYHQGFSSNFAVDDKNVHLTLAFNPSHLEIVSPVVI
                     GSVRARQERMRDIEHEKVLAVTVHGDSAVAGQGVVQETLNMANVRGYKVGGTIRIVIN
                     NQIGFTTSNPNDTRSTEFCTDIAKMIQAPIIHVNGDDPEAVAFAARMAVEYRTKFKRD
                     IFIDLISYRRHGHNEADEPLATQPMMYSIIKKHPTPRKVYADRLITEGVLTNEQEIEM
                     LNLYRDALDNGECVVPEWREMDMAAVDWLQYLNYDWTALYESKFPEERFLTIAKRVCE
                     YPASLKAHSRVEKIYTDRKEMYEGKKLFDWGMAETMAYATLLDEGTHVRLSGEDAGRG
                     TFFHRHAVIHNQNDGTGYVPLTQLHANQGRFQVWDSVLSEEAVLAFEYGFATTDPKTL
                     TIWEAQFGDFANGAQIVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARVERY
                     LQLCAQQNIQVCVPSTPAQVYHMLRRQAIRKMRRPLVAITPKSLLRHPLAVSSLDELI
                     NGSFQNVIGELDNIDPKQVKRVVMCSGKVYYDLIEKRRENNQTDVAIIRIEQLYPFPL
                     EEMQQILEPYAHVKDFVWCQEEPLNQGAWYCSKHNFEASLPEDAKLTYAGRPASASPA
                     VGYPSLHAKQQKQLVEDALTIAE"
     gene            3779..5008
                     /gene="odhB"
                     /locus_tag="PXD71_001646"
     CDS             3779..5008
                     /gene="odhB"
                     /locus_tag="PXD71_001646"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDL6291748.1"
                     /translation="MTIEILTPDLPESVADATVATWHKKVGDNVKRDEILVEIETDKV
                     VLEVPASSDGILAEITQEQGATVVSKQSLGKLVVAKAGDISSATIEQKTESTPSDRKH
                     AAIENSHANADDQGPAIRRLLAEHDLQAADIQGSGVGGRITREDIEKEIAKRVQINQV
                     KGSATSQNTVSTVAFNSRSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNELDMQPIM
                     NLRKTYGEKFEKQHGVRLGFMSFYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIA
                     VSTPRGLVTPVLRDCDKMSMADIEKKIKELAEKGRDGKLTVEDLTGGNFTITNGGVFG
                     SLMSTPIINPPQSAILGMHAIKDRPVAIDGQVVIRPMMYLALSYDHRLIDGKESVGFL
                     VAVKELLEDPTRLLLEI"
     gene            5301..6461
                     /gene="sucC"
                     /locus_tag="PXD71_001647"
     CDS             5301..6461
                     /gene="sucC"
                     /locus_tag="PXD71_001647"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDL6291749.1"
                     /translation="MNLHEYQAKQIFKQYGLPVSQGIACKTAEEAVEAIKQLQGEQWA
                     IKCQVHAGGRGKAGGVKLVRNEAEVREFADKWLGKRLVTFQTDANGQPVNTLYLEETC
                     NIDRELYLGAVVDRSAQKVIFMASSAGGMNIEEVAEKTPELIHKAVIDPLTGGQGYQG
                     RELAFKLGLKGDEVKQFADIFVKLANLFVEKDLALLEVNPLVITKEGQLLCLDAKMSI
                     DSNALYRHPELKELQDPSQDDEREAEAEKWNLNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLYGGKPANFLDVGGGATKERVAAAFKLILSDPNVKAVLVNIFGGIVRCDLIADGVIA
                     AVNEVGVNVPVIVRLEGTNAAQGREILANSGLSILAASSLKQAAELAVKSAN"
     gene            6470..7339
                     /gene="sucD"
                     /locus_tag="PXD71_001648"
     CDS             6470..7339
                     /gene="sucD"
                     /locus_tag="PXD71_001648"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDL6291750.1"
                     /translation="MSILIDKNTKVICQGFTGGQGTFHSEQALAYGTQLVGGVSPNKG
                     GTTHLGLPVFNTVREAVEATGATATVIYVPAPGCKDAILEAIDAGIQLIVCITEGIPT
                     LDMLQVKQRLNQTGVRMIGPNCPGVITPDECKIGIMPGNIHKKGNIGIVSRSGTLTYE
                     AVKQTTDEGFGQSTCVGIGGDPIPGSNFIDILKLFQDDPETEAIVMIGEIGGSAEEEA
                     AAFIKENVTKPVVSYIAGVTAPKGKRMGHAGAIISGGKGTADEKFKILEDAGVTTVRS
                     LAEIGSALRKILG"
     gene            complement(7410..8855)
                     /locus_tag="PXD71_001649"
     CDS             complement(7410..8855)
                     /locus_tag="PXD71_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL6291751.1"
                     /translation="MDSIILFFESVLTWIVNTIDGPLWDVTIILLLGAGLFFTLTTGF
                     VQLRLLPQSLREMWGGRSTEGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAV
                     FWMWITALMGMSSAFAESSLAQLYKTKDPNGMFRGGPAYYITRGLKLPSLAIAFAVTL
                     IFTFGFAFNAVQSNSIVEATKNAWDWNPVYVGMTLVVITGLIISGGVKRIGKVSAQVV
                     PMMALFYLIIAVIILGMNIEKVPSIISLIIESAFDFSAAAGGTFGALVSKTMLMGIKR
                     GLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTIVVCTCTAVIILMSNNYGG
                     ETLRGISLTQSALQFHLGEFGLHFLAFVILLFCFTSIIGNYAYAETNIRYIKNSPWFV
                     WGFRAIVLFFVYFGAVRDGGIVWAFADTVMASMAVINLVAILILAPIVWIILKDYLRQ
                     VKSGKEPVFRIEEHQDLIHKGVDPLIWKSKE"
BASE COUNT         2768 a         1920 c         1875 g         2301 t
ORIGIN      
        1 tacaaaatat taatttatct atttatccta actctatcac gacgatagtc gggccaaatg
       61 gtggaggaaa atctaccctg cttaaaattt tgctaaaatt acttagccca aataaaggaa
      121 aggttattca tcaaaaagga ctcaaaattg gctatgtacc acaaaaattg catttagaac
      181 cctcgatgcc cattactgtt tatcgatttc ttgcgctaaa accacatact accaaagaaa
      241 tgattaacca agcactagat ctgttttcta ttacccatct cactgaaaat agtatgcaaa
      301 aattatcggg tggtgaactt caacgtgtat tgctggctcg ggcaatttta gatcgtccgc
      361 aactcttagt gttagatgaa ccaatgcaag gcgttgatat tataggccaa accgaactct
      421 atcaattact taataaaaca agggatctcc ttaactgtgc tattttaatg gtctcacacg
      481 acttaaatat tgtgatggcg aatacagatg aagtgctttg catcaataaa catatttgtt
      541 gtgccggaag ccccgaagtc attacacacg atccgagctt tatccatttt tttggcgacc
      601 aatttgcacg taatgttgca ttttattcac ataaacataa ccatcaccat aatttacatg
      661 gcgatatctg ccttggtgaa tgtagttgtg agcactgata ggcgtccttt ttaccaaatg
      721 tttaaaattt gtaaaaataa tgctaaaaat aaccgcttgc agcacaagat ttatctaaaa
      781 atcagactta cttcacattt tatttgctta aattggagta taataagggc gtgatttcat
      841 accacaaaca ggtcaggtct ttatgcaata tacacatttt gatgattggc ttgcatcatc
      901 tgcattaggc ggtgcaaacc aatcttatat agaagaaatt tatgaacagt atctagaaaa
      961 tccagcaaat gttgatgaaa gctggagaaa catctttgat tcatttccta aaactcccca
     1021 agttgaacaa gctcacaccc ctattcgtga ctatttccgt aaattagccc gtgaacagca
     1081 acctcaatcc gtcactatta ttgacccaga agcaggggcg aaacaagttc gcttactgca
     1141 atggattaac gctcaccgtt tccgtggcta tttagaggca aaactagacc caattaacta
     1201 ttaccgttgg aaaacctcca gcgtgcctga attggactac aaatatcacg gcttcaccga
     1261 tgaagatctc aacgaaactt ttacggtcgg tcgctatgtc tatgataaag acacaatgaa
     1321 gctcagcgaa ctggataaag ccctaaaaga aacttattta gggcctattg gtttagaatt
     1381 tatgcacgtt caagatattg aacaacgcaa ctggcttcaa gccaaaatgg aaagcgtgct
     1441 aaacaaaccg ctgttcacca aagcagaaaa actgaattta ttaagcgaat tgaccgctgc
     1501 cgatggttta gagcgttact taggggcaaa attcccgggg gcaaaacggt tctcattgga
     1561 aggtagtgat gcctttattc cgatgatgaa agaaattatc cgccacgctt cccgtcaagg
     1621 tatgaccgat gtggttttag gtatggcaca ccgtggtcgt ttgaatatgt tagtgaacgt
     1681 atttggtaaa cgcccggcag aattgtttga tgaatttgcc ggcaaacacg ctgatgaaag
     1741 ccgcacgggc gatgtgaaat accaccaagg tttctcctca aactttgccg tagatgataa
     1801 aaacgttcac cttacccttg ctttcaaccc atcgcattta gagattgtta gcccggtggt
     1861 aatcggctca gtgcgtgcaa gacaagaacg tatgcgtgat atcgaacacg aaaaggtgct
     1921 tgcggtgacc gttcacggtg actctgcagt ggcagggcaa ggtgtagtgc aagaaacctt
     1981 aaatatggca aatgttcgtg gctataaagt aggaggcact atccgtattg tgatcaacaa
     2041 ccaaatcgga tttaccacct ctaacccaaa tgacacccgc tccaccgaat tttgtaccga
     2101 cattgccaaa atgattcagg caccgattat tcacgttaat ggtgatgatc cggaagctgt
     2161 tgcctttgca gcgagaatgg ctgtggaata tcgtactaaa tttaaacgtg atatttttat
     2221 tgatttaatc tcttaccgtc gtcacggtca taatgaagcc gatgagcctt tagcaactca
     2281 gccaatgatg tatagcatca tcaaaaaaca cccgacacct cgcaaagtct atgcggatcg
     2341 tttaattacc gaaggtgttt taaccaacga gcaagaaatc gagatgttaa acctgtatcg
     2401 tgatgcctta gacaacggcg aatgcgttgt gccggaatgg cgtgaaatgg atatggcagc
     2461 ggtcgattgg ctacaatatc tcaattacga ctggactgcc ctatacgaaa gtaaattccc
     2521 tgaagaacga ttcttaacca ttgccaaacg ggtgtgcgaa tatccggctt cgcttaaagc
     2581 tcattctcgg gttgaaaaga tctacactga tcgcaaagag atgtatgaag gcaagaaatt
     2641 attcgactgg ggtatggctg aaacaatggc atacgctact ttgttagacg aaggcacaca
     2701 tgttcgcctt tcaggagaag atgccggacg tggcactttc ttccatcgcc atgcggtaat
     2761 ccataaccaa aatgatggaa caggctatgt gccacttact caactgcacg ccaatcaagg
     2821 gcgtttccaa gtatgggatt ccgtactctc tgaagaagcg gtactcgcct ttgaatatgg
     2881 ctttgccact accgacccga aaacactgac catttgggaa gctcaatttg gagatttcgc
     2941 taacggagct caaattgtta tcgaccaatt tatcagctca ggcgaacaaa aatggggcag
     3001 aatgtgcggt ttagtgatgt tattaccaca cggctatgaa ggtcaagggc cggaacactc
     3061 ctctgcccga gtggaacgct atttacagct ttgtgctcag caaaatattc aagtttgcgt
     3121 gccatcaaca ccggcacagg tttaccatat gttacgccgt caagcgatcc gcaaaatgcg
     3181 tcgcccattg gttgcaatca caccaaaatc attgctacgc cacccacttg cggtgtcatc
     3241 tcttgatgaa ttgattaacg gttcattcca aaatgtgatt ggcgagctcg ataatatcga
     3301 tcctaaacaa gtcaaacgtg tggtgatgtg ttcgggtaaa gtgtactacg acttaattga
     3361 aaaacgccgt gaaaataacc aaacggacgt tgccattatt cgtatcgaac agctctaccc
     3421 gttcccactt gaagaaatgc aacaaattct cgagccttac gctcacgtta aagactttgt
     3481 ctggtgtcaa gaagagccac taaaccaagg tgcgtggtat tgcagcaagc acaattttga
     3541 ggcttctctg ccggaagatg caaaattaac ttacgcaggc agaccggctt ctgcttcacc
     3601 ggcggtgggt tatccatcgt tacacgcaaa acaacaaaaa caacttgttg aagatgcttt
     3661 aacgatcgct gaataataaa atgggcgtat atacgcccta caagcggttc acttttaacc
     3721 aaattttaca atttacgctg tcggagtttt aaaactctct cgaaaaaagg aataagttat
     3781 gaccattgaa attttaaccc ccgatttacc cgaatctgtt gccgatgcga ccgttgccac
     3841 ttggcataaa aaagtgggcg acaatgtaaa acgtgatgaa attttagtcg aaattgaaac
     3901 agacaaagtc gttttagaag taccggcttc aagcgatggt attcttgcag aaattaccca
     3961 agaacaaggt gctactgttg tatccaagca atctctgggt aaattagtag tagctaaagc
     4021 cggtgatatt agctctgcaa ctattgagca aaaaacagaa tccacgccat cagaccgcaa
     4081 acacgcagca attgaaaaca gccacgcgaa cgctgatgat caagggcctg cgattcgccg
     4141 tttattagcc gaacacgact tacaagccgc tgatattcaa ggcagtggtg taggcggcag
     4201 aatcacccgc gaagatattg aaaaagagat tgccaaacga gtgcaaatca accaagtaaa
     4261 agggtctgca accagccaaa acaccgttag cactgtagca tttaacagcc gtagcgaaaa
     4321 acgtgtgccg atgacacgac tccgcaaacg tattgccgaa cgcttactag aggcaaaaaa
     4381 caccactgca atgctcacta cttttaacga gctggatatg caaccaatta tgaatttacg
     4441 caaaacctat ggcgaaaaat tcgaaaaaca acacggtgtg cgtttaggct ttatgtcgtt
     4501 ctacattaaa gccgtggttg aagcattaaa acgctaccct gaaatcaatg cctccattga
     4561 tggcgatgat gtggtttatc ataactattt cgacatcagc attgcggttt ctaccccacg
     4621 aggcttagtc acacctgtgt tgcgtgattg cgacaaaatg agtatggcag atattgagaa
     4681 gaaaattaaa gaacttgccg aaaaagggcg tgacggcaaa ttaaccgttg aagacttaac
     4741 cggcggtaac tttaccatca ccaacggcgg ggtattcggt tcattaatgt ccaccccaat
     4801 tatcaacccg ccacaaagtg cgattttggg tatgcacgct attaaagacc gccctgtcgc
     4861 cattgacgga caagtagtga tccgcccgat gatgtaccta gccttaagct atgaccaccg
     4921 tttaattgac ggtaaagaat ctgtcggttt cttagtggca gttaaagagt tattagaaga
     4981 cccaacccgt cttcttttgg aaatttaatc gaaaatatga ccgcttgtac ttttagctac
     5041 aagcggtctg tttttaataa aatttgacaa aatccgataa acgctctatt tgagttccct
     5101 tctgagccac ctgagcgact ttgaatttgt aggaaaaatt tagtgtttga gcttacaagg
     5161 caagcgagtt ataaattttt ccgtcaagca aattcaaact aagcgaagaa agtggcgaag
     5221 cagggtgtgc tttcttttgg ttacttttct ttgcacaagc aaagaaaagt aacataaaaa
     5281 aaaccaaacg aggaaactct atgaatctac acgaatatca agcaaaacaa atattcaaac
     5341 aatacgggct tcccgtaagc caagggattg cttgcaaaac ggcagaggaa gccgttgagg
     5401 caattaaaca actacaaggt gaacaatggg cgataaagtg ccaagttcac gctggtgggc
     5461 gtggcaaagc cggcggggtg aaactggttc gcaatgaagc cgaagtgcgt gaatttgccg
     5521 ataaatggct cggcaaacgc ttagttacct tccaaaccga tgctaatggg caaccggtta
     5581 ataccttata tttagaagaa acctgcaata tcgaccgtga attgtactta ggtgcagtgg
     5641 ttgatcgctc cgctcaaaaa gtgattttta tggcatcaag tgccggtggt atgaatattg
     5701 aagaagtagc ggagaaaacc cctgaattaa ttcacaaagc ggtaattgac ccactcaccg
     5761 gtgggcaggg ttatcaaggg cgtgaattag catttaaatt aggcttgaaa ggtgatgaag
     5821 ttaaacaatt tgcggatatt ttcgtgaaac tggctaacct atttgttgaa aaagatttag
     5881 ctttattaga ggtaaatcca cttgtcatca ctaaagaagg gcaactactc tgcttagatg
     5941 caaaaatgtc gattgacagc aatgctctct accgccaccc agaacttaaa gagttgcaag
     6001 acccatcgca agatgacgaa cgtgaagctg aagcggaaaa atggaatctc aactacgttg
     6061 cactagatgg caatatcggc tgtatggtaa acggtgcggg gctggcaatg gggacgatgg
     6121 atatcgtcaa actttatggt ggtaaaccgg ctaacttctt agacgttggc ggtggtgcaa
     6181 ccaaagagcg tgtagcagca gcttttaaat tgattttatc tgatccgaat gtgaaagccg
     6241 tgttagtcaa tatttttggt ggtatcgtcc gctgcgattt gattgctgat ggcgtcattg
     6301 ctgctgtaaa cgaagtcggc gtgaatgtgc ctgttattgt gcgtttagaa ggcactaatg
     6361 ccgcacaggg gcgtgagatt ttagccaata gcgggctgag tattctagcg gcatcatcat
     6421 taaaacaagc ggctgaatta gcggtaaaat ctgcaaatta ggaggaaaaa tgtcgatttt
     6481 aattgataaa aacacaaaag taatttgcca aggtttcacc ggcggacaag gtactttcca
     6541 cagcgaacaa gcccttgctt atggcacaca attagtcggc ggagtttcgc cgaataaagg
     6601 tggcacaacc catttaggct taccggtttt caacaccgta cgcgaagcgg tagaagcaac
     6661 cggtgcaact gcaaccgtta tctacgttcc ggctcccggc tgtaaagatg ctattttaga
     6721 agccattgat gccggcatcc aactaattgt ctgtattact gaaggcattc caaccttgga
     6781 tatgctacaa gttaaacaac gtctaaacca aaccggcgtg cgaatgatcg gtccgaactg
     6841 cccgggtgtg attacccctg atgagtgcaa aatcggcatt atgccgggca atatccacaa
     6901 aaaaggcaat atcggtattg tttctcgttc gggtacttta acctatgaag cagttaaaca
     6961 aacaactgac gaaggctttg gtcaatctac ttgtgtcggt atcggtggtg atccgattcc
     7021 gggatctaac tttattgaca ttttaaaact gttccaagac gatcctgaaa ctgaagcaat
     7081 tgtgatgatt ggtgaaatcg gcggctctgc cgaagaagaa gcggcagctt tcatcaaaga
     7141 aaatgtcacc aagcctgtag tcagctatat tgccggtgtc accgcaccaa aaggcaaacg
     7201 aatgggacac gccggtgcga ttatcagcgg cggcaaagga accgccgatg aaaaattcaa
     7261 aatccttgaa gatgccggtg tgacaaccgt cagaagttta gccgaaatcg gttcagctct
     7321 tagaaaaatt ttaggctaaa ctaaaagttc aagcagtcat atttacccat ttttttgtaa
     7381 atatgaccgc ttgttttttt gctattaaat tactctttcg atttccaaat taatgggtca
     7441 acacctttat ggataaggtc ttgatgttct tcaattctaa acactggctc tttgccactt
     7501 ttcacttggc gaagatagtc ttttaaaata atccagacaa tcggagcaag aattaagata
     7561 gccaccaagt taattaccgc cattgatgcc atcactgtat cggcaaatgc ccatacaatc
     7621 cctccgtctc ttactgcacc gaagtacaca aagaataaaa cgatggcacg gaatccccac
     7681 acaaaccacg gactgttttt aatgtagcgg atattagttt ctgcataagc atagttaccg
     7741 ataatggatg tgaagcaaaa cagtaaaatc acaaatgcta aaaagtgtaa accaaattca
     7801 cctaaatgga attgtaacgc actttgagta agtgaaatac ctcttaaagt ttcaccacca
     7861 tagttatttg acataagaat gatcactgca gtacaagtac agactacaat tgtatctaca
     7921 aatacgccca acatttgaat taagccttga cttgccgggt gtttcgcatc agaagtcgct
     7981 gcagaatttg gtgcagatcc cataccagct tcgtttgaga ataagccacg tttaatcccc
     8041 attagcatag ttttggatac caatgctcca aacgttccac ctgcagcagc agaaaaatca
     8101 aaggcgcttt caataattaa agagattata cttggcactt tttcaatatt catacctaaa
     8161 ataattactg caataatcaa gtagaataat gccatcattg gaacaacttg ggcagaaact
     8221 ttaccaatac gcttgacccc tccagaaata attaaccctg taatcaccac taatgtcatg
     8281 cctacgtaga ctgggttcca atcccacgca tttttggttg cttccacaat agagttagat
     8341 tgcacagcat taaaagcaaa accaaaggtg aagataagcg tcactgcaaa agcaatagca
     8401 agagaaggca gttttaaacc acgagtgatg taataggctg gcccaccacg aaacattccg
     8461 tttggatctt tggttttata caactgagcc agtgaagact cagcaaaggc ggaactcatt
     8521 cccattaagg cggtaatcca catccagaat accgcacccg gcccacccaa agcgattgca
     8581 gtagctaccc caccaatatt accaacccca acacggctgg ctaatccagt agtaaaggct
     8641 tggaacggag ttaatgattt cccttccgta gaacgtcctc cccacatttc acgtaaactt
     8701 tgcggaagaa gacgcagctg gacaaaacct gttgtaagag tgaaaaataa ccctgcaccg
     8761 agcaatagga taatcgttac atcccaaagt ggaccgtcga ttgtgttcac aatccaagtc
     8821 agtacactct caaaaaatag aataattgaa tccataaaga cctc
//
LOCUS       DANLXS010000095         8646 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.095, whole genome shotgun sequence.
ACCESSION   DANLXS010000095 DANLXS010000000
VERSION     DANLXS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.095"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(93..479)
                     /gene="rplQ"
                     /locus_tag="PXD71_001650"
     CDS             complement(93..479)
                     /gene="rplQ"
                     /locus_tag="PXD71_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL6291752.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMASALVEHEIIKTTLPKAKEL
                     RRVVEPLITLAKADSVANRRLAFARTRNVDTVAKLFNELGPRFAQRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRPEVATEAAAE"
     gene            complement(515..1504)
                     /gene="rpoA"
                     /locus_tag="PXD71_001651"
     CDS             complement(515..1504)
                     /gene="rpoA"
                     /locus_tag="PXD71_001651"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL6291753.1"
                     /translation="MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVQNKDDIILTL
                     NKSGIGPVTAADITHDGDVEIVNPDHVICHLTDKNASINMRIRVQRGRGYVPASARLH
                     TQNDDRPIGRLLVDARFSPVDRIAYNVEAARVEQRTDLDKLVIEMETNGTLDPEEAIR
                     RAATILAEQLAAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAETIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIAED"
     gene            complement(1535..2161)
                     /gene="rpsD"
                     /locus_tag="PXD71_001652"
     CDS             complement(1535..2161)
                     /gene="rpsD"
                     /locus_tag="PXD71_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL6291754.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIESKCRNRLDVAPGQHG
                     ARKPRLSDYGSQLREKQKVRRIYGILERQFRNYYTEANRLKGNTGENLLVLLEGRLDN
                     VVYRMGFAATRAEARQLVSHKSIVVNGRVVNIPSFQVSVDDVVAVREKSKKQARIKAS
                     LELATQREKPTWLEVDATKMEGVFKRRPERSDLSADINEHLIVELYSK"
     gene            complement(2190..2579)
                     /gene="rpsK"
                     /locus_tag="PXD71_001653"
     CDS             complement(2190..2579)
                     /gene="rpsK"
                     /locus_tag="PXD71_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL6291755.1"
                     /translation="MAKTPVRARKRVKKQIADGVAHIHASFNNTIVTITDRQGNALAW
                     ATAGGSGFRGSRKSTPFAAQVAAERCAEMVKEFGLKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(2596..2952)
                     /gene="rpsM"
                     /locus_tag="PXD71_001654"
     CDS             complement(2596..2952)
                     /gene="rpsM"
                     /locus_tag="PXD71_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL6291756.1"
                     /translation="MARIAGINIPDQKHTVIALTAIYGIGKTRAKAICAATGIAEDVK
                     IRELSEEQIEKLREEVGKFTVEGDLRREVTLSIKRLLDLGCYRGLRHRRSLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(3090..3203)
                     /gene="rpmJ"
                     /locus_tag="PXD71_001655"
     CDS             complement(3090..3203)
                     /gene="rpmJ"
                     /locus_tag="PXD71_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL6291757.1"
                     /translation="MKVRASVKRMCRNCKVIKREGVVRVICSDPKHKQRQG"
     gene            complement(3228..4547)
                     /gene="secY"
                     /locus_tag="PXD71_001656"
     CDS             complement(3228..4547)
                     /gene="secY"
                     /locus_tag="PXD71_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL6291758.1"
                     /translation="MAKQPGYQGSTQKGTGELKSRLLFVLGALIVFRIGSFIPVPGID
                     ASVLSELIRQQQGTIIDMFNMFSGGALSRASIFALGIMPYISASIIIQLLTAIHPPLA
                     ELKKEGEAGRRKISKYTRYGTLLLAFIQSIGISLALPNMVHGLVPNPSILFYVTSVVS
                     LVTGTMFLMWLGEQITERGIGNGISLIIFTGIVADLPATIGQTIEQARQGELSFIVLL
                     LVAIIAFAVTYFVVFVERGQRRIVVNYASRQQGRMMAPARTSHLPLKVNMAGVIPAIF
                     ASSIILFPASITQWFGQSEGFEWLFNLSQLLQPGQPLYVVLFTMAVIFFAFFYTGMQY
                     NPRDTADNLKKSGAFVPGYRPGEQTSRYIDKVMTRLTLIGALYIAFVCLVPYIITSLW
                     KVPFQLGGTSLLIVVVVIMDFIAQVQSHLMSQQYDSVLKKANLKGLN"
     gene            complement(4550..4984)
                     /gene="rplO"
                     /locus_tag="PXD71_001657"
     CDS             complement(4550..4984)
                     /gene="rplO"
                     /locus_tag="PXD71_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL6291759.1"
                     /translation="MRLNSLSPAEGAKHSAKRLGRGIGSGLGKTGGRGHKGQKSRTGG
                     GVRRGFEGGQMPLYRRLPKFGFTSLKSFHVAEIRLNDLAKVDGNEVTLETLKAANIIT
                     KDILSAKVILAGKIERAVVVKGLRVTKGAKAAIEAAGGSIEE"
     gene            complement(4989..5165)
                     /gene="rpmD"
                     /locus_tag="PXD71_001658"
     CDS             complement(4989..5165)
                     /gene="rpmD"
                     /locus_tag="PXD71_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL6291760.1"
                     /translation="MAKIKVTQTRSSIARLPKHKATLKGLGLRHIRHTVELEDTPAVR
                     GMINQVSYMVKVEE"
     gene            complement(5172..5672)
                     /gene="rpsE"
                     /locus_tag="PXD71_001659"
     CDS             complement(5172..5672)
                     /gene="rpsE"
                     /locus_tag="PXD71_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL6291761.1"
                     /translation="MSNIEKQAGELQEKLIAVNRVSKTVKGGRIMSFTALTVVGDGNG
                     RVGFGHGKAREVPAAIQKAMEKARRNMINVALNEGTLQHPVKGSHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVRNVLSKAYGSTNPINVVRATIDALENMKSPEMVAAKRGKT
                     VEEILG"
     gene            complement(5687..6040)
                     /gene="rplR"
                     /locus_tag="PXD71_001660"
     CDS             complement(5687..6040)
                     /gene="rplR"
                     /locus_tag="PXD71_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL6291762.1"
                     /translation="MDKKVARIRRASRARHLMREQGATRLVVHRTPRHIYAQVIAPNG
                     SEVLAAASTVEKVIKEQVKYTGNKEAAAVVGKIVAERAIAKGIKAVAFDRSGFKYHGR
                     VQSLADAAREAGLQF"
     gene            complement(6055..6588)
                     /gene="rplF"
                     /locus_tag="PXD71_001661"
     CDS             complement(6055..6588)
                     /gene="rplF"
                     /locus_tag="PXD71_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL6291763.1"
                     /translation="MSRVAKAPVNIPAGVEVKLNGQLLTVKGKNGELSREIHNAVEVK
                     QEADALTFAPREGIANADAQAGTARALVNNMVIGVTEGFTKKLQLVGVGYRAQMKGNA
                     VALSLGFSHPVEHTLPAGVTGECPSQTEIILKSADKQLIGQVAADIRAYRKPEPYKGK
                     GVRYSDEVVRTKEAKKK"
     gene            complement(6605..6997)
                     /gene="rpsH"
                     /locus_tag="PXD71_001662"
     CDS             complement(6605..6997)
                     /gene="rpsH"
                     /locus_tag="PXD71_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL6291764.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKVAISMPSSKLKVAIANVLAEEG
                     YIESVKVVEGVKPELEITLKYFQNKPVVESIQRVSRPGLRIYKRKDELPKVMGGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(7034..7339)
                     /gene="rpsN"
                     /locus_tag="PXD71_001663"
     CDS             complement(7034..7339)
                     /gene="rpsN"
                     /locus_tag="PXD71_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL6291765.1"
                     /translation="MAKQSMIARDVKRAKLADKFYAKREELKKIISDVNASDEDRWAA
                     VLKLQTLPRDSSPIRQRNRCRQTGRPHGVLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(7351..7890)
                     /gene="rplE"
                     /locus_tag="PXD71_001664"
     CDS             complement(7351..7890)
                     /gene="rplE"
                     /locus_tag="PXD71_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL6291766.1"
                     /translation="MAKLHDYYRDTVVNELKAKFNYSSVMQVPRIEKITLNMGVGEAL
                     TDKKLLDNAVADLAAISGQKPLVTKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLNAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTSA
                     KTDEEGQALLAAFNFPFRK"
     gene            complement(7908..8219)
                     /gene="rplX"
                     /locus_tag="PXD71_001665"
     CDS             complement(7908..8219)
                     /gene="rplX"
                     /locus_tag="PXD71_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL6291767.1"
                     /translation="MAAKIRQNDEVIVLTGKDKGKRGKVTKVLPNGKVFVEGINIITK
                     HEKPVPALGKAGGLVKKEAAIDVSNVAIFNPETNKADRVGFRFEDGKKVRFFKSNEKT
                     I"
     gene            complement(8230..8601)
                     /gene="rplN"
                     /locus_tag="PXD71_001666"
     CDS             complement(8230..8601)
                     /gene="rplN"
                     /locus_tag="PXD71_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL6291768.1"
                     /translation="MIQEQTMLDVADNSGARSVMCIKVLGGSHRRYAAIGDIIKVTVK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGAVIRFDGNACVILNNNTEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
BASE COUNT         2484 a         1930 c         1560 g         2672 t
ORIGIN      
        1 actataaaat aaaattattt tttgatcaaa acgaataaaa gaataaaacc ccggtatttc
       61 taccagggtt ttaagctcaa ttttgattaa gattattccg cagctgcttc agttgcaact
      121 tctgggcgat ccactaactc aatgtaagcc attggagcat tatcacctgc acggaaacca
      181 cattttaaaa tgcgagtgta accacccgca cgttgtgcaa aacgtggacc taattcattg
      241 aataatttag caactgtatc aacgttgcgt gtacgggcaa aagctaaacg gcgatttgca
      301 acgctgtctg ctttagctaa agtaattaac ggctcaacta cacggcgtaa ctctttcgct
      361 tttggtaaag ttgtcttgat gatttcgtgt tcaacaagcg cacttgccat attacggaac
      421 atcgcttggc gatggctgct gttacggttt aattgacgtc cactcttacg atggcgcata
      481 acctattcct tcttagaaaa tctataccga aaaactagtc ttctgcaata cttgcaggtg
      541 gccagttttc aaggcgcata ccgagtgata aaccacgaga agcaagaaca tccttaatct
      601 ctgtaagaga ttttttaccc aagttcgggg ttttaagaag ttcaacttct gtacgttgta
      661 ctaaatcacc gatatagtga attgtctctg ctttcaaaca gttagcagaa cgaactgtca
      721 gctctaggtc atctactgga cgaagaagaa taggatcaaa ttccggtttt tcttctttca
      781 cttcaggttg acgaacatca cgcaaatcaa cgaatgcagc aagttgttct gctaaaatcg
      841 ttgcagcacg acggatagct tcttcaggat ctaacgttcc gttagtttcc atctcaataa
      901 caagtttatc taagtctgta cgttgttcaa cacgagcagc ttcgacattg taagcaatgc
      961 gatctacagg tgaaaaacgt gcatctacta ataaacgacc gatagggcgg tcatcgtttt
     1021 gagtatgaag acgtgctgat gcaggaacat aacctcgacc acgttgaaca cgaatgcgca
     1081 tattaattga tgcattctta tctgttaaat gacagattac gtgatcagga ttcacaattt
     1141 ctacatcgcc atcgtgtgtg atgtcggctg cagttacagg gccaattcca gatttattta
     1201 gtgtcaaaat aatatcatct ttattttgca cttttaccgc tagaccttta aggtttaata
     1261 atacctcaag aatatcttcc tgaacgcctt ctttgctgct gtactcatgc agtacaccat
     1321 caatttcaac ttcagtaact gcacaacccg gcatagatga aagtaaaatg cggcgaagtg
     1381 cgttacctaa tgtatggcca aaaccacgct ctaacggctc taatgtcact ttagcatgag
     1441 ttgaactaac ttgttcaata tttactaagt gcggctttaa aaattctgtc acagaaccct
     1501 gcattttttc ctctctttgc ggttaagcta actattattt agagtaaagc tcaacgatca
     1561 gatgttcatt aatatctgct gatagatcag aacgctctgg acgacgttta aatacgcctt
     1621 ccattttagt tgcatcaact tctaaccaag ttggtttttc acgttgagtt gctaattcta
     1681 atgatgcttt aatacgtgct tgttttttcg atttttcacg aacagcaaca acatcatcaa
     1741 cagaaacttg gaatgaagga atgtttacta cacgaccgtt tactacgatt gatttatggc
     1801 taacaagctg acgtgcttct gcacgtgttg ctgcaaaacc catacgataa actacgttat
     1861 ctaaacgacc ttccaataat actagtaagt tttcaccggt attacctttt aagcggttcg
     1921 cttctgtata ataattacgg aattgacgct ctaagatacc ataaatacgg cgaacttttt
     1981 gtttttcacg taattgacta ccatagtcag ataaacgtgg cttgcgagca ccatgttgac
     2041 caggcgctac atcaagtcta tttctacatt ttgattcaat cgcacgaacg cctgatttta
     2101 agaataaatc agtaccttca cgacggctta gcttgagctt aggacccaaa tatcttgcca
     2161 ttttctttct ccaattcttt taacgtcaat taaacacgac gttttttcgg tggacgacaa
     2221 ccgttatgtg gaatcggagt cacatcagta atattcgtga tacggaaacc tgctgcattt
     2281 aatgcacgga ttgttgactc acgacctgga cccggtcctt taaccataac ttccaaattc
     2341 tttaaaccaa actctttaac catttctgca caacgctctg cagcaacttg agcagcgaat
     2401 ggagtcgatt tacgagaacc acggaaacct gaaccacctg cagttgccca agctagagca
     2461 ttaccttgac ggtcagtaat agtaacgatt gtgttattga aagatgcgtg gatatgagct
     2521 acgccatctg caatctgttt ttttacgcgc ttacgtgcgc gaactggtgt tttagccatt
     2581 atttatctac tccgactatt ttttgatcgg tttgcgtgga cctttacggg tacgcgcatt
     2641 agtcttagta cgttgaccac gtaccggtaa actacgacga tgacgtaaac cacggtagca
     2701 acctaagtct aaaagacgtt tgatgcttaa agttacttca cgacgtaaat ccccttcgac
     2761 agtaaattta ccaacttctt cacgcagttt ttcaatctgc tcttcagaca attctctgat
     2821 ctttacatct tcagcaatac ccgttgcagc acagatggct tttgcacgag ttttaccgat
     2881 accgtaaatt gcagttaatg cgattacagt gtgtttttga tcaggaatgt taatgcctgc
     2941 aatacgggcc actatgcact cctattattt aagtttattg atatactgat cttgaaaagc
     3001 ccgtttcagg atactcaaac agtatatcag gacgaatgag ctgagcagta taactgctca
     3061 gcgggttctt tgcaagaaat atgcgtaaat taaccttgac gttgtttgtg tttaggatcg
     3121 ctacaaatta cacgaaccac accttcacgt ttgattactt tacaattacg gcacattctt
     3181 tttactgaag cacgaacttt cattgcttat cctttattaa cggcctatta atttagacct
     3241 tttaagttag ctttcttcaa cacagaatca tattgttgag acattaaatg actctgaacc
     3301 tgtgcgatga aatccataat aacaaccact acaatcaata atgatgtacc acctaattgg
     3361 aatggcacct tccataacga cgtaatgatg taaggaacca aacaaacaaa agcaatatat
     3421 aacgcaccaa ttaaggttaa acgtgtcatt actttatcga tataacgaga tgtttgttcg
     3481 cctggtcggt aacctggtac aaacgcacct gatttcttaa gattatccgc tgtatcacga
     3541 ggattatact gcattcctgt ataaaagaaa gcaaagaaaa tcactgccat tgtgaataat
     3601 acaacataca gtggttgacc tggctgtaat agttgtgaaa ggttaaataa ccactcaaat
     3661 ccttcgcttt gaccaaacca ttgagtaatg cttgccggga ataaaataat acttgaagca
     3721 aaaattgccg gtattacccc tgccatattc acttttaatg gtaagtgaga agttcttgca
     3781 ggcgccatca ttcgaccttg ttggcgactt gcatagttta caactattct tctttgtcca
     3841 cgttcaacaa aaacaacaaa ataagttact gcaaatgcaa ttattgcaac tagtagtaag
     3901 acgataaatg ataactcgcc ttgacgggct tgttctattg tctgccctat cgttgctgga
     3961 agatctgcaa caatacctgt aaaaataatc aatgagatac cgtttccgat accacgctca
     4021 gtgatttgtt cgcctagcca cattaggaac attgttcctg tcactaagct tactacagag
     4081 gttacataga ataatatact tggattagga actaatccat gtaccatatt cggtagggct
     4141 agtgaaatac caatagattg aataaatgct aataacagtg tgccatagcg tgtatattta
     4201 ctgattttcc gacgacctgc ttcaccttct ttctttaatt cagctagagg tgggtgaatc
     4261 gctgtcaata attggataat aattgacgct gaaatataag gcataatacc tagtgcaaat
     4321 atagatgcac ggcttaaagc accaccagag aacatattaa acatatcaat gatggtgcct
     4381 tgctgttgtc gaattaattc ggataataca gaagcatcaa tgccaggaac aggtataaaa
     4441 gaaccgatac ggaaaacgat taacgcacct aaaacaaaca atagtcttga tttaagctcg
     4501 ccagtccctt tttgtgtact accttggtac cctggttgct ttgccatctt tattcctcga
     4561 tagaaccgcc agcagcttca atagcagctt tagcgccttt agttacacgt aagcctttaa
     4621 caactactgc tctttcaatt ttgcctgcta aaattacttt agcagataaa atatctttag
     4681 taatgatgtt tgctgctttt aatgtctcta aagttacttc gttgccatct actttagcta
     4741 agtcgtttaa acgaatttca gccacatgga atgattttaa tgaagtaaaa ccaaattttg
     4801 gcaaacgacg atataaaggc atttgaccac cctcgaaacc acgacgaaca ccgccgcctg
     4861 tacgagattt ttgaccttta tgaccgcgcc caccagtttt acctaaacca gaaccaatac
     4921 cacgacctag acgttttgca ctgtgcttag caccttcagc tggagaaaga gaatttaaac
     4981 gcattctatt actcctctac tttaaccata taagacactt gattgatcat accacgcact
     5041 gctggtgtat cttcaagttc tacagtatga cgaatatgac gaagacctaa gccttttaac
     5101 gttgctttat gtttcggtaa acgagcgata gagctacgag tttgagttac tttaattttt
     5161 gccatatcca attaccccaa aatttcttca actgttttgc cacgttttgc agcaaccatt
     5221 tcaggtgatt tcatgttctc aagtgcatcg attgttgcac gaacaacgtt aattgggtta
     5281 gttgaaccat acgctttaga aagaacgtta cgaacacccg caacttctaa tactgcacgc
     5341 attgcaccac cagcgatgat acctgtacct tcacttgctg gttgcataaa tacgcgtgaa
     5401 cctgtatgtg aacctttaac tggatgttgt aatgtacctt catttaaagc tacattaatc
     5461 atattgcgac gagctttttc catcgctttt tggattgctg ctggaacttc acgtgcttta
     5521 ccgtgaccaa aacctacacg accgttacca tcgcccacta ctgttaaagc agtgaaactc
     5581 ataatacgac caccttttac ggtttttgaa acacggttaa ccgcgattag cttttcctgc
     5641 agttcaccag cttgtttttc gatgtttgac atctcaaatt cctctattag aactgtagac
     5701 cagcttcacg agcagcatct gctaatgatt gcacacgacc gtggtattta aaaccagaac
     5761 ggtcaaaagc aaccgcttta atacctttgg caattgcacg ctcagcaacg attttaccca
     5821 ctactgctgc ggcttcttta ttgccggtat atttaacttg ctctttaatt actttttcaa
     5881 cagttgaagc tgctgctagt acttctgagc cattaggtgc aatcacctgc gcatagatat
     5941 ggcgaggtgt gcgatgcaca actaaacgtg ttgcaccttg ctctctcatt aaatgacgtg
     6001 cacggcttgc acgacggata cgagctactt tcttatccat agtgttacct tactttactt
     6061 tttcttcgct tcttttgtac gcactacttc atcagagtaa cgtacacctt tacctttata
     6121 aggttcaggt ttgcgatatg cacgaatatc tgctgccact tgaccaatta actgtttgtc
     6181 agcacttttc agaataattt ctgtttgtga tggacattca ccagttacgc ctgctggcaa
     6241 cgtgtgttct actgggtgag agaaacctaa acttaaagca acagcattgc ctttcatttg
     6301 tgctctataa ccaacaccca ctaattgcaa tttcttagta aagccttcag taaccccgat
     6361 taccatatta tttactagtg cacgagcagt acctgcttga gcatctgcat tagcgatacc
     6421 ttcacgtgga gcgaaagtta atgcatctgc ttcttgtttt acttcaactg cattgtgaat
     6481 ttcacgagat aactcgccgt ttttaccttt aactgttaat agctgaccgt taagttttac
     6541 ctcaacaccg gcaggaatat taacaggtgc ttttgcaaca cgagacattt ttcctacctc
     6601 tctattatgc tacgtaacag ataatttcac cgccgaggcc cgcttgacgc gcagcacggt
     6661 cggtcattac acctttagat gtagaaacaa ctgcgatacc taaaccaccc attacttttg
     6721 gtaattcgtc tttgcgttta taaatacgca gacccggacg gctcacacgt tggatacttt
     6781 ctacaaccgg tttgttttgg aaatatttta aagtaatttc cagttcaggt ttaacacctt
     6841 caacaacttt aacgctttcg atataacctt cttcagctaa aacatttgca atagcaactt
     6901 ttaacttaga tgaaggcata ctgattgcaa ctttgttcgc agcttgaccg ttacgaatac
     6961 gggtcagcat atctgcgatt ggatcttgca tgctcatgta tttactccca tgattccaaa
     7021 ttaaaagagg ttattaccag cttgccttct taagacccgg aatttcaccg cgcatagcag
     7081 cttcacgaac cttaatacgg cttaaaccaa acttacgaag tacgccatgt ggacgaccag
     7141 tttggcggca acgattacgc tgacggattg ggctagaatc acgaggaagt gtttgtaatt
     7201 ttaacactgc tgcccaacgg tcttcatcag atgcattcac atcagagatg attttcttta
     7261 attcttcacg ttttgcgtag aatttatcag ctaatttagc acgttttaca tcgcgtgcga
     7321 tcattgattg ttttgccacg ataacctacc ttatttacgg aatgggaaat tgaaagcagc
     7381 taataaagct tgaccttctt catcagtttt cgctgaggtg gtgatagtaa tatctaaacc
     7441 acgaacacga tctactttat cgtagtcgat ttcagggaaa atgatttgtt cacgaacacc
     7501 catactgtaa ttaccacgac catcgaatga cttagcgttt aaaccgcgga agtcacgaat
     7561 acgtggaaca gcgatagtaa tcaatctttc aaagaattcc cacatacgtt caccacgtag
     7621 ggttactttg caaccgattg gatacccttg acggatttta aagcctgcaa cagatttgcg
     7681 agctttagtt actaaaggtt tttgaccgct gattgctgct aaatccgcta ctgcgttatc
     7741 taacagtttt ttgtcggtca atgcttcacc cacacccatg ttaagggtaa tcttttcgat
     7801 tcgtgggact tgcatgacag atgagtagtt gaattttgct tttaattcat taactactgt
     7861 atctctgtag taatcatgca gtttcgccat cacattactc cagttaatta aattgttttc
     7921 tcgttagatt tgaagaaacg cacttttttg ccatcttcga atctaaatcc tacacggtca
     7981 gctttgttgg tttcagggtt gaagatcgca acgtttgaaa catcgattgc agcttctttc
     8041 ttcactaaac caccagcttt gcctaatgca ggaactggtt tttcgtgttt agtgatgatg
     8101 ttgataccct caacaaaaac tttaccattt ggtaacactt tagttacctt accacgtttg
     8161 cccttatctt taccggtaag aacaattact tcatcgtttt gacggatttt tgcagccatt
     8221 acattctcct tatagtactt ctggagctaa agagatgatt ttcataaact tctcagaacg
     8281 aagttcacga gtcaccggtc caaaaatacg agtaccgatt ggttgctcag tgttattgtt
     8341 taaaattaca caagcattgc catcgaaacg aataactgcg ccatctgggc gacgaacacc
     8401 cttcttggtg cgcacaacaa ctgcttttaa cacatcacct tttttcactt taccgcgtgg
     8461 aattgcttct tttacagtaa ctttaatgat atcgccaata gcagcgtaac gacggtgcga
     8521 tccacctaga accttgatac acattacgct acgagcccct gagttatcag caacatccag
     8581 catagtctgt tcttggatca tattagtgct ccgttaaaat aaaaaaacac cctttcggga
     8641 cgaacc
//
LOCUS       DANLXS010000096         8642 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.096, whole genome shotgun sequence.
ACCESSION   DANLXS010000096 DANLXS010000000
VERSION     DANLXS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.096"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            75..344
                     /locus_tag="PXD71_001667"
     CDS             75..344
                     /locus_tag="PXD71_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL6291769.1"
                     /translation="MKIMAVCGSGLGSSFMMEMNIKKVLAKLGVEAEVSHTDLSSVVA
                     DDADLFVMAKDIAASSNVPKEKLIVVKNIVGLAEFEEKLTAYFNQ"
     gene            357..1707
                     /locus_tag="PXD71_001668"
                     /pseudo
     CDS             357..1707
                     /locus_tag="PXD71_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(1802..2950)
                     /gene="dprA"
                     /locus_tag="PXD71_001669"
     CDS             complement(1802..2950)
                     /gene="dprA"
                     /locus_tag="PXD71_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL6291770.1"
                     /translation="MAYHYDLLRLLQVPQMGAQRIGRLLAELNFAEFCHYDKLQLKQI
                     GWNEKQIQRWFNPDQKWIDQAMTWSELPNQQIVTLFDENYPFLLKQISTAPPLIYVKG
                     NVESLSLPQIAIVGSRDYSHYGEYWAGQFAAELARHNIIITSGLAIGIDGFAHQKIVM
                     EQGITIAVLGSGLNQVYPARHKKLAEQIVDLGGALVSEFSPNQPPVAENFPRRNRIIS
                     GLSLGTLVIEATLNSGSLITARYALEQGREVFALPNAVQNPYAQGCHKLIKEGALLVE
                     GVEDILEVVASQYQPQQIPLFDESKPTVSTSLQAVKNSEKFAKNLPSLTACQQQIYQH
                     ISLEPMAIDDLAKVTELTIEILLVELLGLELAGVIKQVSGGYVMDNGY"
     gene            3039..3551
                     /gene="def"
                     /locus_tag="PXD71_001670"
     CDS             3039..3551
                     /gene="def"
                     /locus_tag="PXD71_001670"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL6291771.1"
                     /translation="MAILDVVLYPDEKLATVCEPVEQVDEALNRFIDDMFETMYEHEG
                     IGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESSGETGIEEGCLSIPGHRALVP
                     RKEKVKVKALNRQGEEIIIDADDLFAICIQHEIDHLNGVLFVDHISNLKRQRIKEKMQ
                     KLKKQIARAK"
     gene            3645..4571
                     /gene="rfaD"
                     /locus_tag="PXD71_001671"
     CDS             3645..4571
                     /gene="rfaD"
                     /locus_tag="PXD71_001671"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDL6291772.1"
                     /translation="MIIVTGGAGMIGSNIVKALNEIGRKDILVVDNLKNGEKFINLVD
                     LDIADYCDKEDFIASIIAGDDFGEIDAVFHQGACSATTEWDGKYLMQNNYEYSKELLH
                     FCLDRQIPFFYASSAATYGGRSDNFIEKREFEGPLNAYGYSKFLFDEYVRRILPEAES
                     PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFAGSEQFLRDFVYVEDVA
                     KVNLWAWQNSISGIFNLGTGKAESFQEVANAVIKYHGKGEIETIPFPDHLKSRYQTFT
                     QADLTNLRAAGYTGTFKTVAEGTAEYMAWLNR"
     gene            complement(4639..5169)
                     /gene="fabA"
                     /locus_tag="PXD71_001672"
     CDS             complement(4639..5169)
                     /gene="fabA"
                     /locus_tag="PXD71_001672"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDL6291773.1"
                     /translation="MNSCTPIIKPSYTYEELLLSGKGELFGEKGPQLPAPPMLMMDRI
                     IEMNQEKGNFEKGYIEAEFDIKPDLWFFNCHFINDPVMPGCLGLDAMWQLVGFYLGWI
                     GGEGKGRALGVGEVKFTGQILPTAKKVTYRIHMKRVVNRKLVMGVADGEVEVDGRVIY
                     TATDLKVGLFQDTTAF"
     gene            complement(5282..6919)
                     /locus_tag="PXD71_001673"
     CDS             complement(5282..6919)
                     /locus_tag="PXD71_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6291774.1"
                     /translation="MTLSLLSSQKLVMQNDFSRLDEKLNFLDFQPQAKQALNQFLQSQ
                     FGALLVLKSELFPEFINEITEYLKKYSVDLPIISKMDFNKNSLFGYSVFLERELKTEY
                     VKGAIHEADKGILIVNIRSLLLDITQWDKLKQALLFGEYEPNSVNHSPYLSAKEKSTF
                     KLILVGEREDISTLSAYDDGLYQFGQYTEIKSYLSLSDNVEQWGGYVQNYAENLINKR
                     LTSTALNQFLQAYIRESESRELVSISPTLLRKHLLGIQNFYPNVTEIDEVKGYFDYLE
                     QQSSILNQYTVGDILTNQLYIETEGEEIGQINGLSVIEFEGIPHSFGEPLRMSCNVQY
                     GDGEITDIERKVELGGNIHSKGIIIAQSCLANLLELPTQLPFSASIAFEQSYGEIDGD
                     SSSLAIFNVLVSALAKVGLPQSIAVTGAIDQFGNVLSVGGVNQKIEGFFNICHARQLT
                     GKQGVIIPAACVSHLSLSSEVVEAVEQGLFKIWTVENVFDAIAILFERDFYDDEKAPN
                     SAQTALFTLIHQNLDERESEETSGSFLSKICKKIKLD"
     gene            7012..7458
                     /gene="matP"
                     /locus_tag="PXD71_001674"
     CDS             7012..7458
                     /gene="matP"
                     /locus_tag="PXD71_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDL6291775.1"
                     /translation="MRYQKLITQETTWKWQYLLKKHREGENITKYEEQSLIDLKVQFL
                     STLQNSPDEVEKWIKSEMTPEQRKKMRQSVRAKRKRFFNAEKQTTKKKSIDLEYSSWL
                     RLSKYAKSQKLTLSEAIVKLTDELENKQLYLEQVAKMKSSLKDLLK"
     gene            complement(7524..>8642)
                     /gene="ompA"
                     /locus_tag="PXD71_001675"
     CDS             complement(7524..>8642)
                     /gene="ompA"
                     /locus_tag="PXD71_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119863613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDL6291776.1"
                     /translation="ALAVLSAAAVAQAAPQANTFYAGAKAGWASFHDGLTQFDNTYDY
                     GSGINLGINLGINRNSVTYGVFGGYQILNQNNFGLATELGYDYFGRVRGNTTFSGDDK
                     EKTAAKHSAHGAHLSLKPSYEVVPNLDVYGKVGVALVRNDYYVQQHVDKDSRIKVHNL
                     KPSLLLGAGLEYAITPELAARVEYQYLNRVGNLDKAVRTTVGYLIPRGTNFQYSPDIH
                     SVSAGLSYRFGQGAAPVEAPEVVTKNFAFSSDVLFDFGKSSLKPAAATSLDAAHAEIS
                     NLGLANLAIQVNGYTDRIGKEASNLKLSQRRAETVANYIVSKGTNPANVTAVGYGEAN
                     PVTGHTCDAVKGRKALIACLAPDRRVEIQVQGSKEVTM"
BASE COUNT         2601 a         1789 c         1650 g         2602 t
ORIGIN      
        1 acatttctat tctatagtat ttctatagtt caaaattcag acaactattc attcaccttt
       61 aatggagatt tactatgaaa attatggcag tatgtggttc cggcttaggt agcagcttta
      121 tgatggaaat gaacattaaa aaagtattgg caaaactcgg tgtcgaagcg gaagtgagtc
      181 ataccgacct ttcctctgtt gttgctgatg atgctgattt atttgtgatg gcaaaagata
      241 ttgccgcaag cagcaatgtg ccgaaagaaa aactcatcgt ggtaaaaaac attgttggat
      301 tagcagaatt cgaagaaaaa ttgaccgctt actttaacca ataacggaga tgttatatgg
      361 aatcgctact cttttttata ttagatattc tcaaagtgcc ttccgttcta gtgggtttaa
      421 ttgctctggt cggtttagtt gcacagaaaa aatcggtatc cgatattgtt aaaggaacaa
      481 tcaaaactat tttaggcttc ttagtgttaa gcggtggtgc aacagtactt ttaagctccc
      541 tgactccttt aggcggtatg tttgaacacg ccttcaacgt gcaaggcatt atccctaata
      601 acgaagcgat tgtgtctatg gcaatcgaaa aatatggtac ggcgactgcg ttaattatgg
      661 cattcggtat ggtagccaat attttaattg cacgttttac ccgcctaaaa ttcattttct
      721 taaccggaca ccataccttc tatatggcgt gtatgatcgg cgtaatccta accgttgccg
      781 gctttgaagg tattcagctc gtctttgtcg gggcattaac cttagggtta attatgtttc
      841 ccgactatcg ctcacttcta tatgaaaaaa gtgaccgcta gcaatgatgt tgccttaggt
      901 catttcggca cattgggcta cgtgcttgct ggttcaatcg ggcaactggt aggaaaaggc
      961 tctaaatcta ccgaagaaat ggatttacct aaaaacctcg gcttcttacg tgatagctcc
     1021 atttctattt cactcacgat gatggcgatt tactatattc tcgcccttgc ttcgggcagt
     1081 gaatacgttt ctacattaag cggcggacaa cattatttag tgtatgccac cattcaagcc
     1141 atcacttttg cagccggtgt ttatgtcatt ttgcaagggg ttcgtttaat tttagccgag
     1201 atcgtcccgg catttaccgg cttctccgaa aaattagtac ctgatgccaa accggctctc
     1261 gactgcccga ttgttttccc ttacgccccc aatgcggtat tagttggatt tcttgccagt
     1321 ttcttaggtg ggattttaag cttagccatt ctagggcaac ttaattgggt gttaatttta
     1381 ccgggcgtag tgccacactt cttctgtggg gcaactgccg gcgtatttgg gaacgccacc
     1441 ggcgggcgtc gtggtgcaat tatcggtgct tttattcacg gcatattaat taccttcttg
     1501 cctgtattct tattacctgt actgggttca ttaggctttg ctaacaccac tttctctgac
     1561 tcagacttcg gcggtattgg gattgtgtta ggctatatgg ctcaatactt cgacaagaat
     1621 atcattacgc tcattattgt cggcttattc gctctgttgg tagcctataa ttatttggcg
     1681 aaaaaaccgg caaaactgaa tacctaatta aaatcaggtg ttatttgctg cctgcgagga
     1741 atcttccctg ccatttcttg atggtaggga attttgttta gttaaacgac aacgctcttg
     1801 ttcaataccc attatccatc acataccccc cactcacttg tttaatcaca ccggctaatt
     1861 ccagacccag taattcaacc agcaatattt caatcgtcaa ctccgtaact ttggctaaat
     1921 catcaattgc catcggctct aagctgatat gctgataaat ttgctgctgg caagcggtca
     1981 gacttggcag attttttgcg aatttttcgc tgtttttgac cgcttgtaaa gatgtgctta
     2041 ctgttggctt agactcatca aacaaaggga tttgctgagg ttgatattgc gaggcgacca
     2101 cctctaaaat atcttccaca ccttccacga gcaatgcccc ttctttaatt agcttgtggc
     2161 aaccttgtgc gtaagggttt tgtaccgcat tcggtaacgc aaacacttcc ctaccttgtt
     2221 caagggcata acgagcggta atcagtgaac cactattaag cgttgcttca ataactaaag
     2281 tgccgagcga taagccacta ataattcgat tacgacgagg aaaattttct gccaccggcg
     2341 gctgattagg cgagaactcc gacaccaatg caccacctaa atcaacaatt tgttcggcta
     2401 attttttatg gcgagcagga tagacttgat ttaatccact ccctaacacg gcaatcgtta
     2461 tgccttgttc cataacgatt ttttgatggg caaaaccgtc aataccaatg gctaaaccgc
     2521 tggtaatgat aatattatgg cgagctaatt cggcagcaaa ttgcccagcc caatattcac
     2581 cataatgaga atagtctcgg ctgccaacaa tcgcaatttg tggcagagaa aggctttcca
     2641 catttccctt tacataaatt aacggtggcg cagtcgaaat ttgttttaag agaaacggat
     2701 aattttcatc aaagagtgtc acaatttgct gatttggcaa ttctgaccaa gtcatcgcct
     2761 gatcaatcca cttttgatca ggattaaacc aacgttgaat ttgcttttca ttccaaccta
     2821 tttgtttgag ttgaagtttg tcatagtggc aaaattcagc aaaattgagt tccgccaata
     2881 atcgccctat acgttgagcc cccatttgcg gcacttgtaa taatcgtaat aaatcgtagt
     2941 gatatgccat attttcatcc tcaaaatttc cctaataata agaaaaggtt taaaatagcc
     3001 aaacgaattt tttaaacttt tcgagagaga tcacaaaaat ggcaatatta gatgttgtgc
     3061 tttaccccga tgagaaactg gcaaccgttt gtgaaccggt ggaacaggtg gacgaagcac
     3121 tgaatcgttt tatcgatgat atgtttgaga caatgtacga acacgaaggt attggcttag
     3181 ctgcaccgca agtgggcgta cttaagcgtg ttattactat tgatattgag ggcgacaaaa
     3241 ccaatcaagt ggtgttaatt aaccctgaaa ttttagaaag cagcggtgaa accggcattg
     3301 aagagggctg tctctccatt cccgggcatc gtgccttagt gccacgcaaa gagaaagtga
     3361 aagtcaaagc ccttaaccgc caaggcgaag aaattatcat cgatgccgat gatttatttg
     3421 cgatttgtat tcaacacgaa atcgaccatt taaacggcgt gctttttgtt gaccacatat
     3481 ccaatttaaa acgccaacgc atcaaagaaa aaatgcaaaa attgaaaaag caaattgcac
     3541 gagcgaaata accaggcaca agcggttaaa atggcttaaa aatttgcaaa ttaagtgtga
     3601 tgacacgata ataaccacag ttcaacttta gaaaggataa aaatatgatt atcgtcaccg
     3661 gcggcgcagg tatgattggc agtaacattg ttaaagcctt aaatgaaatc ggtcgcaaag
     3721 atattttagt agtagataac ctcaaaaacg gggaaaaatt tatcaactta gtggacttag
     3781 atattgcgga ttattgcgat aaagaggatt tcatcgcctc tattatcgcc ggcgatgatt
     3841 ttggtgagat tgatgcggtt ttccatcaag gggcttgctc ggcaaccacc gaatgggacg
     3901 gcaaatactt aatgcaaaat aactacgaat attcaaaaga gttattgcat ttttgcttag
     3961 atcgccaaat tcctttcttc tatgcctcaa gtgcggcaac ctatggcgga cgttcggata
     4021 attttatcga gaaacgggag tttgaaggtc cattaaatgc ttatggctac tccaaattcc
     4081 tgtttgatga atatgtccgc cgtattttgc ctgaggcaga atcaccggtc tgtggtttca
     4141 aatatttcaa cgtatacggg cctcgtgagc aacacaaagg ttcaatggca agcgtggcat
     4201 tccacttaaa caaccagatg ttaaaagggg aaaatccaaa actatttgcc ggctcagagc
     4261 agttcttgcg tgattttgtt tatgtggaag atgtggcaaa agtgaatctt tgggcgtggc
     4321 aaaacagcat ttctggcatt ttcaacctcg gcaccggaaa agcggaatct ttccaagaag
     4381 ttgcgaatgc ggttatcaaa taccacggca aaggcgaaat tgaaaccatc ccattcccgg
     4441 atcatttaaa atcccgctac caaacgttca ctcaagcgga cttaaccaac ctaagagccg
     4501 ctggctacac aggcacattt aaaaccgttg ctgaaggaac agcggaatat atggcgtggt
     4561 tgaatcgcta atcaaacgtt gttaaaaaag cagaaagagc tacgaaatgt agctcttttt
     4621 gttataaggt tataaggatt agaatgctgt cgtatcttgg aataagccta cttttaaatc
     4681 agtagcagta tagatcactc gcccgtccac ttcgacttca ccatcagcaa cccccatcac
     4741 taacttacga ttgaccacac gtttcatatg gatacggtaa gtaacttttt tcgctgtcgg
     4801 taaaatttgt ccggtgaatt ttacttcacc tacgcccaat gcacgccctt tgccttcgcc
     4861 accaatccag ccgagataaa aaccaaccag ttgccacatc gcatctaagc ctaagcaacc
     4921 cggcattaca ggatcgttaa taaaatggca gttaaaaaac cataaatccg gtttaatatc
     4981 aaactcagct tcaatatacc ctttttcaaa attgcctttt tcttggttca tttcaatgat
     5041 gcgatccatc attaacatcg gtggagccgg caattgaggc cctttttcac cgaataattc
     5101 tccttttcct gatagcaaaa gctcttcgta agtatagctt ggtttgataa ttggtgtaca
     5161 actgttcatt atttttaaaa tcctgtaaag tgaaaaaagc ctgttatatc aataactgat
     5221 aaatcaggct gatagtaaca gacttttaat tcagtgaaca gttgtaagct gacttgtcgg
     5281 atcagtctag ctttatcttt ttgcaaattt tgcttaaaaa tgaaccgctt gtttcttcac
     5341 tttcccgctc atctagattc tggtgaatca aggtaaataa tgctgtttgg gctgagttag
     5401 gggctttctc atcatcataa aaatcccgct caaacagaat cgctatggca tcaaacacat
     5461 tttccaccgt ccaaatttta aatagacctt gctccactgc ctcaacaact tctgaactta
     5521 aactcaaatg gctaacacag gccgccggaa taatcacacc ttgtttcccc gttaattggc
     5581 gagcgtgaca aatattaaag aagccttcaa ttttctgatt taccccgccc acgcttagca
     5641 cattcccaaa ttgatcgata gcacctgtca ccgcaataga ttgtggcaag ccaactttag
     5701 ctaaggcact cactagtaca ttgaaaatcg caagagaaga actatcccca tcaatttcac
     5761 cataggattg ttcaaaagca atcgaagctg aaaacggtaa ctgggttggt agttctaaca
     5821 gattcgctaa acaagattga gcgataataa tacctttgga gtggatattt ccccctagct
     5881 ccactttacg ctcaatatct gtaatttcgc catcaccata ttgtacatta caactcatgc
     5941 gtaagggctc gccaaaacta tgcggaatgc cttcaaactc aatgaccgat aagccattga
     6001 tctgcccaat ttcctctcct tcggtttcaa tataaagttg attagtcaga atatcaccca
     6061 ccgtatattg atttaatatc gatgattgct gttctaaata atcaaaataa ccttttacct
     6121 cgtcaatttc ggtcacattt gggtaaaaat tttgaattcc taataaatgt tttctcaata
     6181 atgtagggga aatagacacc aactctcggc tttcactttc acgtatatag gcctgtaaaa
     6241 attgattaag cgctgtggaa gttaatcgct tattgatgag attctcagca taattctgaa
     6301 cataacctcc ccactgctcc acattatccg ataatgataa gtaggatttt atttcggtat
     6361 attgcccaaa ttggtacaac ccatcatcat aagcagataa agtagaaata tcttctcttt
     6421 cccctactaa aatcaattta aatgttgatt tctctttagc cgataaatag ggtgagtgat
     6481 taacagaatt tggctcatat tcaccaaata ataatgcctg ttttagctta tcccattggg
     6541 taatatccaa taagagcgaa cggatattaa caattaaaat tcccttatct gcctcgtgaa
     6601 ttgcaccttt aacatattct gttttcagct ctctttccaa aaacacagaa taaccaaata
     6661 gactattttt attgaaatcc attttgctaa taatcggcag atctacacta tattttttca
     6721 gatattccgt gatttcattg ataaattccg gaaaaagctc agattttaag actaataaag
     6781 caccaaactg agattgtaaa aattgattta acgcttgttt tgcttgaggt tgaaaatcta
     6841 aaaaattcaa tttctcatct aaacgagaga aatcattttg cattactaat ttttggctac
     6901 taagtaaaga aagggtcaca aatctgcctc attattgaaa atttggttta ttatacaaaa
     6961 ttccgctata cttgctaaat atagcttact ttttcctatc atattcagat tatgcgatat
     7021 cagaaactca tcacccaaga aacaacttgg aaatggcaat atctcctaaa aaaacatcgt
     7081 gaaggagaaa atattactaa gtacgaagaa caaagcttga tcgatttaaa agttcagttc
     7141 ctctctactt tgcaaaactc tccggacgaa gtggaaaaat ggattaagtc ggaaatgacg
     7201 cctgagcaac gtaagaaaat gcgtcaatca gtacgcgcca aacgcaaacg cttctttaat
     7261 gctgaaaagc aaacaaccaa gaaaaaatcc attgatttag agtattcgag ctggttaagg
     7321 ctctctaaat atgccaaaag ccaaaaacta acgctttctg aagctattgt caaattaacc
     7381 gatgaactcg aaaataaaca actttactta gagcaagttg ctaaaatgaa atcaagttta
     7441 aaagatttac tgaaataaaa aaagaaaaaa gcccactaac aagcgggctt tttttgattc
     7501 aatttttcaa ctgaataact atattacata gttacttctt ttgaaccttg aacttggatt
     7561 tcaacacgac gatctggtgc taagcaagcg attaatgctt tacgaccttt aactgcatca
     7621 catgtgtgac cagttactgg gtttgcttca ccgtaaccta cagctgttac attagctggg
     7681 ttagtacctt tagaaacgat gtaattagct actgtttcag cacgacgttg tgaaagtttt
     7741 aagttagatg cttctttacc gatgcggtct gtgtaaccat taacttggat agctaggttt
     7801 gctaagccta agttgctgat ttcagcgtgt gctgcatcta aagaagttgc tgctgctggt
     7861 tttaagcttg atttaccgaa atcaaataaa acatcagagc tgaatgcgaa gtttttagtt
     7921 acaacttctg gtgcttcaac tggtgctgca ccttgaccga aacggtatga taaacctgca
     7981 gatactgagt ggatatctgg gctgtattgg aaatttgtac ctcttggtat tagataacca
     8041 actgttgtac gaactgcctt atctaagtta ccaacacgat ttaagtattg atattcaaca
     8101 cgagcagcta attcaggagt aattgcatac tcaagaccag cacctaataa tagagatggt
     8161 tttaagttgt gaactttaat gcgtgaatct ttatctacat gttgttgtac gtagtaatcg
     8221 ttgcgaacta atgcaacacc tactttaccg taaacatcta agttaggaac aacttcgtag
     8281 cttggtttta agcttaagtg cgcaccgtga gctgaatgtt tagccgcagt tttttcttta
     8341 tcatcaccag aaaatgtagt gttaccacga acacgaccaa agtagtcata acctaattct
     8401 gtcgctaaac caaagttgtt ttggtttaag atttggtaac caccgaatac accgtaagtt
     8461 acagagttac ggttaatacc gaggttaata ccgaggttaa taccggatcc ataatcatat
     8521 gtgttgtcaa attgagttaa accgtcgtgg aatgatgccc aaccagcttt agcacctgcg
     8581 tagaaagtgt tagcttgtgg agctgcttga gctactgcag ctgctgataa tactgctaat
     8641 gc
//
LOCUS       DANLXS010000097         8392 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.097, whole genome shotgun sequence.
ACCESSION   DANLXS010000097 DANLXS010000000
VERSION     DANLXS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.097"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            84..1460
                     /locus_tag="PXD71_001676"
     CDS             84..1460
                     /locus_tag="PXD71_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDL6291777.1"
                     /translation="MLSNEVFISIIVLLILSLLRINVVIALIISALTCGLIGNLGVEG
                     AGFTEALEKTIKSFTGGLGGGAETAMNYAVLGAFAVALSKSGVTDLLAYKVIKSFGHS
                     PSGRSVTWFKYIVLFVLVAFAMSSQNLIPIHIAFIPILIPPLLSVMNKLQIDRRAVAC
                     ALTFGLTATYMLIPAGFGQIFIESILVKNINQAGEPFQLVTTTAEMTKAMLIPVSGMI
                     LGLLFAFFVSYRKPRVYIENMPEPTADDIEARVANVKPFHIGISIVAIIATCSVQLAT
                     NSTIIGGLVGLVIFALGGVFRLKESNDVFQAGLRLMAMIGFVMIAASGFAGVVNSTGG
                     VPALVESLRDVISSKEMAALLMLVVGLFITMGIGSSFSTVPIITSIYVPLCISFGFSP
                     LATMAIVGVAAALGDAGSPASDSTLGPTSGLNADGKHDHIWDSVVPTFIHFNIPLLVF
                     GWIAAMTL"
     gene            1558..2361
                     /gene="xthA"
                     /locus_tag="PXD71_001677"
     CDS             1558..2361
                     /gene="xthA"
                     /locus_tag="PXD71_001677"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL6291778.1"
                     /translation="MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPY
                     DLVNHLGYHVFHHGQKGHYGVALLTKKEPIAVRKGFPTDVLDAQKRMIMADIETDFGV
                     LTVLNGYFPQGENRSHETKFPAKQKFYADLQNYLESELKPENPIIIMGDMNISPTDLD
                     IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLVDTFRAGNPDVNDKFSWFDYRSK
                     GFDDNRGLRIDLILASKSLAERCVETGIDLDIRAMEKPSDHSPVWATFK"
     gene            2515..3099
                     /gene="lptC"
                     /locus_tag="PXD71_001678"
     CDS             2515..3099
                     /gene="lptC"
                     /locus_tag="PXD71_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDL6291779.1"
                     /translation="MNKRLTVILLIIVAVLGGWYYSLQEKENTGLDQLIKKEGQPEYV
                     GNKMSTTVYDLKGNPQYFAQATEIKRYESTERTEFFKPFIELFAKESTQKQWKVSADY
                     AEITKEKMLNLKGNIKLVALDPASRLQQITTDTLTVDLNTQDIFTESTVKSIGSGFTT
                     TGVGLKGNLKQQVATLQKDVKSYIEPTIIQETKE"
     gene            3123..3629
                     /gene="lptA"
                     /locus_tag="PXD71_001679"
     CDS             3123..3629
                     /gene="lptA"
                     /locus_tag="PXD71_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HDL6291780.1"
                     /translation="MKLMFKSILVSVLLGSSFSAYALKGDTDQPINIDSGSQSLDMTN
                     NIVTFSDNVVITQGSIKVTAANVKITRQEGKKETIDATGSPVTFQQTLDNGKPVNGKG
                     NSVHYDLNSEFLTLIGNAELKQQGSFIKANKITYDVKKQQLKATSGGKTRVKTVLIPN
                     ELQDNKKK"
     gene            3641..4366
                     /gene="lptB"
                     /locus_tag="PXD71_001680"
     CDS             3641..4366
                     /gene="lptB"
                     /locus_tag="PXD71_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005727816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDL6291781.1"
                     /translation="MSVLYAEHLAKSYKQRQVVKDVSLNVKSGEIVGLLGPNGAGKTT
                     TFYMVVGLVRHDHGTIRIDDQDISTLPMHDRAKQGIGYLPQEASIFRRLSVYDNLMSV
                     LQVRKNINDKQRKERAEELITEFHIEHIRNSLGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKKIIVNLKERGLGVLITDHNVRETLDVCERAYIVGSGEMIAS
                     GTPQQVLENPDVKRVYLGDQFKL"
     gene            4408..4920
                     /locus_tag="PXD71_001681"
     CDS             4408..4920
                     /locus_tag="PXD71_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL6291782.1"
                     /translation="MTKLTEFLSPENVRQGVLISSKKRALELVGKIVAESINQTEQQS
                     EALCPIECFGNLFKREKLGTTGLNQGIALPHAKLPACGDIELTKPIAVFLQLEEAIDY
                     EAQDNKEVDLIYAMMFPENSCEQYKSCLPAIAQKLTDKSLLKQLRAAESAEDIWQILV
                     YADNHLKDEE"
     gene            4933..5796
                     /gene="rapZ"
                     /locus_tag="PXD71_001682"
     CDS             4933..5796
                     /gene="rapZ"
                     /locus_tag="PXD71_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL6291783.1"
                     /translation="MELIIISGRSGSGKSVALRALEDAGYYCVDNIPLPLIPTLTYYL
                     TQENRSAVISLDIRNLPNTPNAIEELLTQLEPLNAKLIFLDCERNTLIRRYSDSRRIH
                     PLSTQDDLSLEGALDLEQKLLEPLVQNANYIIDTSHLSSHELAENLRQILQGSTEKEL
                     KIIFQSFGFKYGLPADSDYVFDVRFLPNPHWNPELRPMTGLEQPVIDFLERQTEVHNF
                     IYQTRNYLEMWLPMLEQNNRSYLTIAIGCTGGKHRSIFIAEQLAKYFQAKGKNVQVRH
                     KSLEKHHKKTS"
     gene            5879..7063
                     /locus_tag="PXD71_001683"
     CDS             5879..7063
                     /locus_tag="PXD71_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL6291784.1"
                     /translation="MKSADIVIVGGGMVGLALAGLLKDVDCQIKIIEKNTPKFDADTI
                     YHRVSAINASSQKILEQIGAFQHIPAQRLSPYTEMKVWEKDSFANIHFDNNDNEIKQL
                     GLSQLGFILENAVIQHALWQQVSQQANVEYIIAAPQTLGVSENGAFLTLDNGEMLSAK
                     LVVGADGANSWVRNQMKMPLTSKDYQHTALVCNVKTTEKHGKTARQIFAEDSIFAFLP
                     MADEHLCSIVWSLPPNKANELKNASEAEFNKALTIAFDNQLGLCEVQSLREIYPLAAR
                     FCRDFAQPRIALIGDAAHTIHPLAGLGVNLGFADARTLANEIQKQLVNNQDIGEYRHL
                     RQFERERKAQAMKLLATMEGLKQLFDGNHPLKKLFRGIGLSLTNQLPFVKKQLIQQAI
                     YL"
     gene            7195..7779
                     /locus_tag="PXD71_001684"
     CDS             7195..7779
                     /locus_tag="PXD71_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein HlpB"
                     /protein_id="HDL6291785.1"
                     /translation="MNKLTKISATALVALFLAACDKPAEKAPATEPTKTTEAAPTTTA
                     PAAEAAKPAETAVAAPSEAAKADYQKLVDWNTAQESTMATAQAELQKKLATQDPKQIQ
                     EGLSAFNKKVEETVKSLDAIEVSDAQIKTFKEKTKSVLTLSSEVISEQVKTISTPNDQ
                     AALQAVQQKAQSLIEAGNELQKLNVELQQRFSAK"
     gene            7846..>8392
                     /locus_tag="PXD71_001685"
     CDS             7846..>8392
                     /locus_tag="PXD71_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein Hlp"
                     /protein_id="HDL6291786.1"
                     /translation="MNTLAKISLSVLFTLFLTACEQPNSTKTQSSAESPVQVKEESKE
                     EVKPADTGAQDYKMLREWQDTQEKALNDAIKAATETLTDKQKADSTLMQETVNNALLA
                     QIDHIKISAETLNIQNNEVKALKDKTLEVLTLGAQMIVEGAKMEKNPTPEAHKAFGEL
                     QTKLNQLAEEGQQLENILRAKY"
BASE COUNT         2700 a         1688 c         1667 g         2337 t
ORIGIN      
        1 cctgaattta aaaaaaattc tcgacaatac ttttttttac agtaaattaa acaattattt
       61 aaataaccta aggatcaaac acaatgttat caaacgaagt ctttatttcg attatcgtgc
      121 tgttaatcct aagcttgctt agaattaatg tagtaatcgc acttattatt tctgccctta
      181 cttgcggtct aatcggtaat cttggtgtag aaggggctgg atttaccgaa gcattggaaa
      241 aaacaataaa aagttttact ggtggtttag gtgggggtgc ggaaactgct atgaactatg
      301 ccgttcttgg tgcgtttgcc gtagctctat caaaatcagg cgtgaccgat ttattagcct
      361 ataaagtgat caaatccttt ggtcacagcc cgtcaggtcg ctccgttact tggtttaaat
      421 acattgtatt atttgtgtta gtggcatttg caatgtcatc tcaaaaccta attccaattc
      481 atattgcctt tattccgatt ttaattccac cgctattaag cgtaatgaat aaattgcaaa
      541 ttgaccgccg tgcggtagct tgtgctttaa cctttggttt aaccgccact tatatgttaa
      601 ttccggcagg tttcggtcag atctttattg aaagcatctt agttaaaaat atcaaccaag
      661 caggtgaacc gttccaatta gtgactacca cagcggaaat gaccaaagcg atgttaattc
      721 ctgtttccgg tatgatttta ggcttactgt ttgcattctt tgtgtcatac cgtaaaccgc
      781 gtgtgtatat tgaaaatatg cctgaaccaa ccgcagatga tattgaagcc cgtgttgcaa
      841 acgttaagcc cttccatatc ggcattagta ttgtggcgat tatcgcgacc tgttcggtgc
      901 aattagcaac aaattcgact attatcggcg gcttagtcgg tctggttatt ttcgccttag
      961 gcggtgtatt tagactgaaa gagagtaacg atgtcttcca agctggctta cgtttaatgg
     1021 cgatgattgg ctttgtgatg attgccgcct ccggttttgc cggcgtggtc aattcaacag
     1081 gcggtgtgcc ggcattggta gaaagcctgc gtgatgtgat ttcatcaaaa gaaatggcag
     1141 cattgctaat gttagtcgtt ggcttattta ttacgatggg tatcggctct tcattctcga
     1201 ctgtgccgat cattacctca atttatgtgc cgctttgtat ctcatttggc ttctcgccac
     1261 tggcgacaat ggcgattgtc ggtgttgctg ctgcactcgg tgatgcaggt tctccggcat
     1321 cagattccac acttggtcca acctccggct tgaatgctga cggtaaacac gaccacattt
     1381 gggattctgt tgtaccaact tttatccact ttaacattcc attattagtg ttcggctgga
     1441 ttgcagcaat gacactttaa gctataatat gcaaaatttt tgctaaaaat gaccgcttgt
     1501 agccattaca agcggtctat tttcttcttt attttgcaaa ccacattagg atcaccaatg
     1561 aaatttatct cttttaatat caacggatta cgagccagac cacatcagct cgaagcgatt
     1621 atcgaaaaac accagcccga tgtactcggc ttacaagaaa tcaaagttgc cgatgaagat
     1681 tttccttacg atttagtcaa tcacttaggc taccacgttt tccaccacgg gcagaaaggt
     1741 cattacggcg tggcattact gactaaaaaa gagcctatag cggtgcgtaa aggcttcccg
     1801 acagatgtac ttgatgctca aaaacgaatg attatggcgg atatcgagac cgatttcggc
     1861 gtgctaaccg tattaaacgg ctatttccca cagggggaaa atcgcagcca cgaaaccaaa
     1921 ttcccggcaa aacaaaaatt ctatgctgat ttgcagaact acttggaaag cgaattaaag
     1981 ccggaaaatc cgattattat tatgggcgat atgaacatca gcccgactga tttggatatc
     2041 ggcatcggcg agccaaaccg caaacgctgg ctgcgtgacg gtaaatgttc attcttaccc
     2101 gaagagcgtg aatggctaga tcgcttatac agctacggct tggttgatac tttccgtgca
     2161 ggcaacccag acgtgaacga taagttctct tggtttgact accgttcaaa aggttttgat
     2221 gataatcgtg gcttgcgtat tgatttaatt cttgcttcaa aatcacttgc tgagcgttgc
     2281 gtggaaacag gcattgattt agacattcga gcaatggaaa aaccgtctga ccactcccct
     2341 gtttgggcaa cctttaaata aagttgcaaa aaaatctgag ttttcaaccg cttgtcagtt
     2401 tttagcccta caagcggtta ttttttctca cttttttgca ctttttacgc tgtactatat
     2461 ccctattcaa atctgtgcta caatcaaaca ctatttttag attatttttg gattatgaac
     2521 aaacgtttaa ccgttatttt actgattatt gttgccgtac ttggtggctg gtattacagc
     2581 ttacaagaga aagagaatac ggggcttgat cagcttatta aaaaggaagg acagcctgaa
     2641 tatgtgggga ataaaatgtc caccacggtc tatgatctaa aaggaaaccc acaatatttc
     2701 gctcaagcta ctgaaattaa acgctatgag tctactgagc gtactgagtt ttttaaacct
     2761 tttattgagc tttttgccaa agagtcgacg caaaaacagt ggaaagtcag tgccgattat
     2821 gcagaaatta ccaaagaaaa aatgttaaat ttaaaaggta atatcaaact tgttgcatta
     2881 gatcctgcat cacgtttgca acaaatcact accgatacgt taactgttga tttaaataca
     2941 caggatattt ttaccgaaag tacggtcaaa tcgataggtt ccggttttac tacaacgggg
     3001 gttggattaa aaggtaactt aaaacaacaa gttgcgactt tgcaaaaaga tgtaaaatct
     3061 tacattgaac cgaccattat tcaagaaact aaagaataat ttgcttggaa caaggaataa
     3121 caatgaaatt aatgtttaaa tctattttag tgagcgtgtt actgggtagc agcttttccg
     3181 cttatgctct aaaaggcgat accgatcagc cgattaatat tgattcaggt agccaatcgt
     3241 tagatatgac taacaatatc gtgaccttta gcgataatgt cgttatcact caagggtcga
     3301 ttaaagtgac cgcagcaaac gtcaaaatta cccgtcaaga aggtaaaaaa gaaactattg
     3361 acgctacagg ctcacctgta acattccaac aaaccttaga taacggtaaa ccggtaaacg
     3421 gtaaaggcaa cagcgttcat tatgatttaa actctgaatt tttaacgctg attggcaatg
     3481 cggaactcaa acaacaaggt agctttatta aagccaataa aattacctat gatgtgaaaa
     3541 aacaacaact taaagctacc agtggcggta aaacccgagt gaaaaccgtg ctaattccaa
     3601 atgaacttca agataacaag aaaaaataag gttaaatgat atgtctgtgc tttatgccga
     3661 acatttagca aaaagctata aacaacgcca agtggtaaaa gacgtcagcc tgaatgtcaa
     3721 atcaggtgaa atcgtgggct tgctcggtcc gaacggcgca ggtaaaacca ccactttcta
     3781 tatggttgtg ggtttggttc gccacgacca cggcacaatt cgtattgacg accaagatat
     3841 cagcacacta ccaatgcacg acagagcaaa acaagggatt ggctatctac cgcaagaagc
     3901 atctattttc cgccgtttaa gtgtgtacga taacttaatg tcggtattac aagtgcgtaa
     3961 aaatatcaat gataaacagc gtaaagagcg tgccgaagag ctgattacag aattccatat
     4021 tgagcatatt cgcaatagtt taggtcaatc actttcaggt ggtgaacgcc gccgtgtgga
     4081 aattgcccga gcattagcag cgaacccgaa atttattctg ttagatgaac cttttgctgg
     4141 ggttgaccca atctcggtaa ttgatattaa aaaaattatc gtcaatttaa aagagcgtgg
     4201 tttaggggta ttgattaccg accataacgt acgtgaaact ctagatgtat gtgaaagagc
     4261 ctacattgtc ggtagcggcg aaatgattgc cagcggcaca ccacagcaag tgcttgaaaa
     4321 ccctgatgta aaacgtgttt atttaggcga tcaattcaaa ctctaaaaaa tcccctcttt
     4381 ggggatttca tctttagatg aaaaattatg acaaaactaa ccgaatttct aagccctgaa
     4441 aatgttcgcc aaggtgtact gatttcaagt aaaaaacgag cgttagaact cgtgggtaaa
     4501 atcgtggctg aatcaatcaa ccaaaccgaa cagcaatctg aagcactctg ccctattgaa
     4561 tgctttggca atttatttaa acgtgaaaag ttaggcacaa ccggcttaaa tcaaggcatt
     4621 gcacttcctc atgcaaaatt gcccgcttgc ggtgatattg agctgactaa accgattgcg
     4681 gtctttttac aactggaaga agcgattgac tacgaagccc aagacaacaa agaagtggat
     4741 ttaatttatg caatgatgtt tccggaaaat agctgcgaac agtataaaag ttgcctacct
     4801 gcaattgcac agaaacttac cgataaatcg ttattaaaac agctccgagc agctgaatcc
     4861 gcagaagata tttggcaaat tttggtgtat gctgataatc acctaaaaga tgaagaataa
     4921 tactgaggaa atatggaatt aatcattata agcggacgct caggctctgg taaatcggta
     4981 gcattaagag cattagaaga tgcaggttat tactgcgtgg ataatatccc tttaccttta
     5041 attccaacct taacctacta ccttactcaa gaaaatcgtt cggcagtcat cagcttagat
     5101 attcgtaatt taccgaatac gcctaatgcg attgaagaat tgcttaccca gcttgagcct
     5161 ttaaatgcta agctgatttt tttagattgc gaacgcaata ccttaatccg ccgttatagc
     5221 gattcccgcc gaatccaccc gctttccacc caagacgatc tctcgttaga aggtgcattg
     5281 gatttggaac aaaaattgct tgagcctttg gtgcaaaatg caaattatat tattgacacc
     5341 agtcatcttt cttcgcacga attagcggaa aatcttcgcc aaatactgca aggctcaacc
     5401 gaaaaagagc ttaaaattat tttccaatca ttcggtttta aatacggctt acctgccgat
     5461 tccgattacg tttttgatgt tcgcttttta ccgaacccac attggaatcc ggagcttcgc
     5521 ccaatgacag gcttagagca accggtgatt gatttcttag aacgccaaac cgaagtgcat
     5581 aattttattt accaaacccg caattattta gaaatgtggc tgccgatgtt ggaacaaaat
     5641 aaccgtagct acctaactat tgcgattggt tgcactggtg gaaaacatcg ttccatattt
     5701 attgccgagc aactggcaaa atatttccaa gcaaaaggca aaaatgtgca agttcggcat
     5761 aaaagtttgg aaaaacacca taaaaaaaca tcttaaataa gtaggggctg gtcctgtgcc
     5821 agccctcatt tcaactattt tagggcgggc acagggcacc gcccctacat ttgattttat
     5881 gaaatccgca gatattgtta ttgttggcgg cggtatggtt gggctggctc ttgccggact
     5941 gctcaaagat gtcgattgcc aaattaaaat tattgagaaa aacacgccca aatttgatgc
     6001 cgatacgatt taccaccgtg taagtgcgat taatgcttca agccaaaaaa tactggaaca
     6061 gattggtgct tttcaacaca ttccggctca acgcctttct ccttacaccg aaatgaaagt
     6121 gtgggaaaaa gacagctttg ctaatatcca ttttgataat aatgataatg aaatcaagca
     6181 attaggactt tcacaacttg gctttatttt ggaaaatgcg gtgattcaac acgcattgtg
     6241 gcaacaggtt tctcaacaag ctaatgtaga atatattatc gccgcccccc aaactctggg
     6301 cgtgagcgaa aacggggcat ttttaacctt agataacggc gaaatgctgt ctgctaaatt
     6361 ggttgtcggg gcagacggtg caaattcgtg ggtgcgtaac cagatgaaaa tgccgcttac
     6421 cagtaaagat tatcaacata ccgccttagt ctgtaatgtc aaaacaaccg aaaagcacgg
     6481 caaaaccgct cgtcaaatct ttgctgaaga tagcattttt gcttttctgc caatggcaga
     6541 tgagcatctc tgctcgattg tttggtcgct gccaccaaac aaagccaatg aattaaaaaa
     6601 tgcaagcgaa gccgaattta ataaagcctt aacgattgcc ttcgataatc agcttggctt
     6661 atgtgaagtg caaagtttgc gtgaaattta cccgcttgca gcacgcttct gccgtgactt
     6721 tgcccaacct cgtattgcgt tgattggtga tgccgctcat accattcacc cattggcggg
     6781 acttggtgtc aatttaggct ttgccgatgc cagaacgctg gcaaacgaaa tccaaaaaca
     6841 gttggtaaac aaccaagata ttggtgaata ccgccattta cgccaatttg aacgtgagcg
     6901 taaagcccaa gcgatgaaat tattagccac aatggaagga ttaaaacagc tatttgacgg
     6961 caatcacccg cttaaaaaat tattcagagg aattggttta agcctaacca accagctgcc
     7021 ttttgtgaaa aagcagctta ttcagcaagc tatttactta taaaatgtaa attttttcga
     7081 aataacgtaa agatgaacga ttttatgaac taatacggag attacttgtc tatttattgg
     7141 tacaataacc agcctttatt ggcttttatt tttatattct tgaggaattt tttaatgaac
     7201 aaattaacaa aaatcagtgc aaccgcttta gtcgcattat tcttagcagc ttgtgataaa
     7261 ccagcagaaa aagcaccggc aacagaacct acaaaaacaa ctgaagctgc accaactaca
     7321 acagctcctg ctgcagaagc agccaaacct gctgaaacag ctgtagccgc tccaagcgaa
     7381 gctgcaaaag cagattacca aaaattagtt gattggaata cggcacaaga aagcacaatg
     7441 gcaacagcac aagcagaact acaaaagaaa ttagctacac aagatcctaa acaaattcaa
     7501 gaaggattaa gtgcatttaa taaaaaagta gaagaaactg taaaaagctt agatgcgatt
     7561 gaagtatctg atgcacaaat caaaacattt aaagaaaaaa caaaatcagt tttaacactt
     7621 tcaagtgaag tgatttctga acaagtaaaa acgatctcta caccaaatga ccaagcagca
     7681 ttacaagccg tacaacaaaa agctcagtct ttaattgaag ctggtaatga gttacaaaaa
     7741 ttaaacgtag aattacagca aagattctca gctaaataat ttaccattca atgaaaagct
     7801 agagtatttt gctctagctt ttttttgcaa ttactaagga ttactatgaa cacgctcgct
     7861 aaaattagcc tatctgtatt atttaccctt tttttaaccg cttgcgaaca acctaattct
     7921 accaaaacac agagctcagc agaatcgcca gttcaagtga aagaagagtc aaaagaagaa
     7981 gttaaacctg ctgataccgg cgctcaagat tataaaatgt tacgggaatg gcaagacact
     8041 caagaaaaag ccttaaatga tgcaattaaa gcggcaacag aaacacttac tgacaaacaa
     8101 aaagccgatt ctaccttaat gcaagagaca gttaataacg cattattagc tcaaattgat
     8161 catattaaga taagtgctga aaccctgaat attcaaaata atgaagtcaa agcactaaaa
     8221 gacaaaacct tagaagtcct gactttaggg gctcagatga tcgtagaagg tgctaaaatg
     8281 gagaaaaatc ctacaccgga agcacataaa gcatttggcg agttacaaac aaaattgaat
     8341 caattagctg aagaagggca acagttagaa aatatattaa gagctaaata tg
//
LOCUS       DANLXS010000098         8377 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.098, whole genome shotgun sequence.
ACCESSION   DANLXS010000098 DANLXS010000000
VERSION     DANLXS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.098"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..824
                     /locus_tag="PXD71_001686"
     CDS             <1..824
                     /locus_tag="PXD71_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDL6291787.1"
                     /translation="SQKIYQDKQSYLVEIEKSMNDYAELVKRYEKLLKVGHSSHDEVN
                     IQKSRYFQQKSLFNELKQELIQLKSTQLSLENEIETRKTEFNNQIIRYEMQKSDLNIR
                     LMEFESVSDIIVNASLDGKIESTSVTVGQIIKENDPLAQILPQNKGNYQLVMWVPNSA
                     ISFIKQGDEVNIRYEAFPFEKFGQFKGKITSISTLPASLQELSFYKNLPVNLEQGIPL
                     YKILIELADQNVKYNDTSLYFMSGMKAEATLFLEKRKLYEWIFFPMYQLRKNMEQ"
     gene            824..1615
                     /locus_tag="PXD71_001687"
     CDS             824..1615
                     /locus_tag="PXD71_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HDL6291788.1"
                     /translation="MTGIKQLKFDFRRRIPVILQTETAECGLACLAMMLGYYDKNTNL
                     FELRSQYGTSSRGVTLHTLISIANDCGLITRPLSLELDEVPQLRLPCILHWDFNHFVV
                     LTHASEKQFIIHDPAFGKRKLSRAEFSNHFTGVALEVWSEVKFEKSNNENTISLYETL
                     KHISGIKGALVKIFALSMLIELIALLMPIGTQLVMDHVMQAKDQSLLLIICIGLFLFT
                     LFRTMVSMFRAWVSLKMNYLIDFQWTASFFSHLLKLPLDFFENAK"
     gene            1993..2910
                     /locus_tag="PXD71_001688"
                     /pseudo
     CDS             1993..2910
                     /locus_tag="PXD71_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2912..3580)
                     /locus_tag="PXD71_001689"
     CDS             complement(2912..3580)
                     /locus_tag="PXD71_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL6291789.1"
                     /translation="MKYLYALIFSLFFSSVYAAERQLQCKVVGISDGDTLTCLVNKTP
                     LKIRLQHIDAPEQAQPYGNKAKQALAQFVFQQNITAIVSGYDRYQRLLATLYLNDKNI
                     NLALVENGMAWAYTTKREYIEAQDQAQQAKIGLWQDKFPINPSQWRKTHTSTLPQAVK
                     KPENIANQPPTIDCQIKLSCNQIGNYAQAKRYFQQCGWKELDGNNDSIPCNKLYRQEQ
                     KNRN"
     gene            complement(3624..4991)
                     /locus_tag="PXD71_001690"
     CDS             complement(3624..4991)
                     /locus_tag="PXD71_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDL6291790.1"
                     /translation="MSIDTILNQINSFVWGPPLLILISGVGLYLTFSLKGIQLSQLGQ
                     AFRYMFKPEKGKGQSGDISAFAALSTALAATIGTGNIVGVATAIHSGGPGALFWMWLI
                     ALFGMATKYAEGLLAIKYRTKDRAGFVAGGPMYYIELGMGKKWRWLAKAFAFFGVLVA
                     LLGIGTFPQVNGITHALESTFDVPIIFSAIILTLIVTAIILGGVQRISKIATIVVPFM
                     AIGYVIAALSVILLNMEKVPQAIITIVHAAFNPQAALGGAVGFTVMQAIQLGVARGIF
                     SNESGLGSAPIAAAAAQTKEPVRQGLISMTGTFLDTIIVCTMTGLVIVISGAWAGDVK
                     GAELTTTAFTQGLNSSFGAIIVTVGLVFFAFTTILGWCYYGERCFVYLTRGRTQYIKL
                     YRLLFIALIASAPFLQLDTIWTLADIVNGLMAFPNLIALIALRKVIISETKDFFARLK
                     TGKAE"
     regulatory      complement(5110..5205)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(5376..6428)
                     /locus_tag="PXD71_001691"
     CDS             complement(5376..6428)
                     /locus_tag="PXD71_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL6291791.1"
                     /translation="MTHSTKTLVAIASIIIILAGIKLSNEIVVPFLLSLFIAIICSPM
                     IKFMTKRKVPLWLAITILLIVFILGFSFLAGIINNTLQEFTNAIPQYKIQLSSRIQVI
                     LELAHRWHIPLTLSQEEIMSKFDPSSIMNLVSKLLMGLSGALSNIFVIFLVVIFMLLE
                     MPVAKYKLALALSPDDDLSNEKEYINQVLEGVIGYLGVKTFVSLLTGGLVWILLEILG
                     VEYAILWAILTFLLNYVPNIGSILAGIPIVIQALVLNGFSIGLGTLIGIVVINMVVGN
                     ALEPKLMGKKLGLSTLVVFLSLLFWGWLLGTVGMLLSVPLTMACKVALESNKATQKYA
                     VLFSDLEEVPKEQKYR"
     gene            complement(6475..6900)
                     /gene="ruvX"
                     /locus_tag="PXD71_001692"
     CDS             complement(6475..6900)
                     /gene="ruvX"
                     /locus_tag="PXD71_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL6291792.1"
                     /translation="MTETTTKTVIAFDFGTNSIGCAVGQSITGTAQGLTTFKAQDGIP
                     NWEEIGKLLQDWKPDMLVVGLPLNMDGTEQPLTQRARKFANRLNGRFNLPVELQDERL
                     TTVEAKAEIFSRGGYKALKKDKVDSISACLILESWFENH"
     gene            complement(6900..7460)
                     /locus_tag="PXD71_001693"
     CDS             complement(6900..7460)
                     /locus_tag="PXD71_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDL6291793.1"
                     /translation="MLENLQGKFLIATPEMDDDYFERTVVYICEHNPQGAMGVVINTP
                     TDLSVLELITRMDFQMANQRDYKKDQMVLSGGPVNQDRGFIIHTKTANDFIHTYPITD
                     KITLTTSGDVLDSFGTLASPEKFIVCLGCSTWRGSQLEQEIAQNYWIVSDANEKTLFE
                     TGYLERWAEAYEMMGLSSIPSRAGRA"
     gene            complement(7460..8191)
                     /gene="rsmE"
                     /locus_tag="PXD71_001694"
     CDS             complement(7460..8191)
                     /gene="rsmE"
                     /locus_tag="PXD71_001694"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL6291794.1"
                     /translation="MRIPRIYQPQPLAGLQSCVLSEDAANHVGRVLRMKQGEQIILFD
                     GSNHVFHATLQAVEKKQIIAKIDSSELDDRESNLPIHLGQVISRGDRMEFTIQKSVEL
                     GVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
                     KEQDDMLKLNLHPRAKYTIRELPNVPKAGVRLLIGSEGGLSAEEIAMTESEGFTEVLL
                     GKRVLRTETASLAAITALQVCFGDI"
BASE COUNT         2518 a         1608 c         1661 g         2590 t
ORIGIN      
        1 acagtcaaaa aatttatcaa gataagcaga gttatttagt ggaaattgaa aaaagtatga
       61 atgactatgc ggagttggtt aaacgctatg agaaattatt aaaagtaggg cattccagcc
      121 acgatgaagt gaatattcag aaaagccgtt atttccaaca aaaatcactg tttaatgagc
      181 taaagcaaga attaattcaa ttaaaatcca cccaacttag cttggaaaat gaaattgaaa
      241 cccgtaaaac cgaatttaat aatcagatta ttcgctatga aatgcaaaaa agcgatttaa
      301 acattcgttt aatggaattt gaatctgttt cagatattat tgttaatgcc tctttagatg
      361 gaaaaattga aagtaccagc gtaacagttg ggcaaattat taaagaaaat gacccgcttg
      421 cacaaattct gccacagaat aaaggaaatt atcagctagt gatgtgggta ccgaatagtg
      481 ccatttcatt tattaaacaa ggcgatgagg tgaatattcg ctatgaggct tttccgtttg
      541 aaaaattcgg gcagtttaag ggcaaaatta cgtcaatttc aaccttacca gcttctttgc
      601 aagaacttag tttttataaa aatctccctg taaatttaga gcaaggtatt ccgctttata
      661 aaattctgat tgagcttgcc gatcaaaacg taaaatataa tgatacctcc ctttatttta
      721 tgagtggaat gaaagccgaa gccacgttat ttttagaaaa acgtaaactg tatgagtgga
      781 tatttttccc tatgtatcaa ttacgcaaaa atatggagca ataatgacag gaattaagca
      841 gttaaaattt gattttcgga gaagaatacc tgttattttg caaactgaaa cagctgaatg
      901 tggcttagcc tgtttagcga tgatgttggg ctattacgat aaaaacacca atttatttga
      961 attgcgaagt caatatggta cttcctctcg tggcgtaacg ctgcatactt taatttctat
     1021 tgcaaatgac tgtggtttaa taactcgccc tctttctcta gagttagatg aagtaccaca
     1081 acttcgtctg ccttgtattc ttcattggga ttttaatcat tttgtagtat taactcacgc
     1141 aagtgaaaaa cagtttatta tccacgatcc tgctttcggt aaacgtaaat tatcccgtgc
     1201 tgaattttcc aaccatttta ccggagtcgc tttagaagtt tggtctgaag ttaaatttga
     1261 gaaaagcaac aatgaaaata ccattagttt gtatgaaacc ttaaagcata tttctggcat
     1321 aaaaggtgca ttagtgaaaa tttttgccct ttcaatgctc attgaactca ttgccttatt
     1381 gatgccaatt ggaacacagt tagtgatgga tcacgttatg caagcgaaag atcaatcatt
     1441 acttttaatt atctgtattg ggctattttt atttaccctt tttcgcacaa tggtcagtat
     1501 gtttagggct tgggtttcct tgaaaatgaa ttatttaatt gattttcaat ggacagccag
     1561 tttcttttct catctcttaa aattaccgct tgatttcttt gaaaacgcca agtaggtgat
     1621 attcattccc gatttagttc attaaaaacc attcagaaaa ctctaacttc aagcattatt
     1681 accacgatta ttgatacgat tatgataatc agtttaatta ttatgatggt gttatatggc
     1741 ggttggctct tttgggtagt attagctttt tcctttatct attttattct tcgaatggtt
     1801 acttatttag tttatcgtca actgaatgaa gagcagatta ttaaaggagc aaaagcccat
     1861 tcgcatttta tggaaacttt atatggtatt acaacgttaa aatcattagg attggaaaat
     1921 aaacgacaag aacagtggat gtcgctcaat gcggatgctt ttaacacatc aattaaggta
     1981 actaaatttg atatgctgtt ttccggtatt cataccttta tttctaccat cgaacagatt
     2041 ttaattcttt ggattggggc gacaatgatg attgaaaatg tgatgacatt aggaatgttt
     2101 gtcgctttta atgcctatcg aggctctttc tcttcacgaa tggggaattt aattgatatt
     2161 atttttagcc taaaaattct ttcattacat agagagcgga ttgcagacat tgctttaaat
     2221 gaagtcgaag atgaaagtaa atatcaagaa ctcagatttg aacataacca aacagatata
     2281 gaagttaaag acctttgttt caaatacgat cctttttcta aaaatgtgct tgaaaatgtc
     2341 aatttagtga tcagaagtgg ggaaagtgtt gctattaccg caccatcagg ttatggcaaa
     2401 acaacgttat taaaattaat tgcaggttta ttaaaaccaa cctcaggttc tgtgtatttt
     2461 aacggcattg atatttatca gttaggatta agtcaatatc ggagccaaat tgcttgcgta
     2521 ttacaggaag ataaattctt ttctggttcc attttagaga atattgttag ttttgaaagt
     2581 aattatgatc gagaatgggc tatcgaatgt agcaaacttg cgaatattca caatgatatt
     2641 atggcaatgc cgatgaatta tgaaacctta ttaggagaat taggaaataa tttgtctggc
     2701 ggacaaagat aacgattgtt tattgctcga gctttatata aaaagcctaa aattcttttg
     2761 atggatgagg cgactagcca tttagatgaa gaaaatgaga agcttgtcaa tcaagctatt
     2821 tcacgaatga agataacaag gattattatt gctcatagac aatcgactat tcagtcggcg
     2881 gatagaatta ttgccctgaa tgacaggtaa attaattcct atttttctgt tcttgccgat
     2941 aaagtttatt gcacggaatg ctgtcgttgt tgccgtctag ctctttccag ccgcattgtt
     3001 gaaagtagcg ttttgcctgt gcgtaattgc cgatttggtt gcagcttaat ttaatttggc
     3061 aatcaatcgt tggtggctgg tttgcaatat tttccggttt tttgaccgct tgtggtaagg
     3121 ttgaggtgtg tgtttttcgc cattggctcg ggttaatcgg gaatttgtcc tgccataagc
     3181 cgattttagc ttgctgtgct tgatcttgtg cttcgatata ttcccgcttt gtggtgtaag
     3241 cccacgccat accgttttcc actaaggcta agttgatgtt tttatcgttt aagtagaggg
     3301 tggcaagtaa tcgttggtaa cgatcatagc ctgaaactat ggcagtaatg ttttgttgaa
     3361 acacgaattg agccagagct tgtttggctt tgttgccgta aggctgcgct tgttcggggg
     3421 catcaatatg ttgtagcctt atttttagtg gtgttttatt gaccaaacaa gtgagggtgt
     3481 cgccatcgct aatgccaacg actttgcatt gtagttggcg ttcggcggca tataccgatg
     3541 agaaaaataa gctaaaaatc agggcgtaaa gatatttcat tattaaaaaa gagagggcta
     3601 ataagccctc attctatcat tttttattcg gctttacctg ttttaaggcg agcgaagaaa
     3661 tcttttgttt cactaataat cactttgcgt aacgcaatca atgcgattaa attcgggaat
     3721 gccattaagc cgttcacgat gtctgccagc gtccaaattg tatcgagttg taaaaatgga
     3781 gcagaggcga tgagtgcaat aaacagcaag cggtataatt taatgtattg ggtgcgacct
     3841 cgagtgagat agacaaaaca gcgttcaccg tagtagcacc agcctaaaat ggtggtaaag
     3901 gcaaagaaga ctaaaccaac tgtcacaatt atcgcaccga atgagctgtt taaaccttga
     3961 gtaaaagcgg ttgtggtgag ttctgcgcct tttacatcgc ctgcccaagc ccctgaaatc
     4021 acaattacta agccggtcat tgtgcagacg ataatagtgt ctaagaacgt gccggtcatt
     4081 gaaattaggc cttgacgtac cggctctttg gtttgggcgg cagcggcggc gatgggggca
     4141 gagcctaatc ctgattcatt cgagaaaata ccacgtgcaa cccctaattg aatcgcttgc
     4201 atgacggtga atccgactgc tccgcctaat gccgcttgtg ggttgaaagc tgcgtgtaca
     4261 atagtaataa ttgcttgtgg gactttttcc atattcagta gaatgacgga taaagcggcg
     4321 atgacatacc caatagccat aaacggtaca acaatggttg caattttaga aatccgctgc
     4381 acgccaccta aaataatagc ggtaacgata agggttaaaa tgatcgcact gaagataatc
     4441 ggcacatcaa aggtgctttc taaggcgtgg gtaatgccat tcacttgggg gaacgtgcca
     4501 ataccaagca aagcgaccaa tacgccaaaa aacgcaaagg ctttagcaag ccaacgccat
     4561 ttttttccca ttcccagttc gatatagtac atcggaccac cagccacaaa tccggctcta
     4621 tctttagttc gatatttaat tgccaataag ccttcggcat atttcgtagc cataccgaat
     4681 aaggcaatca gccacatcca aaataatgct ccaggcccgc ccgaatgaat agcggttgcc
     4741 acgccgacaa tattccccgt gccgatagta gctgctaggg cagtggaaag ggctgcaaaa
     4801 gcggaaatat cgcccgattg ccctttgcct ttttccggtt tgaacatata gcgaaaggct
     4861 tgccctagtt ggcttaattg aatacctttg agtgaaaagg ttaaatataa ccctacgcct
     4921 gaaattaaaa tcaggagtgg cgggccccaa acaaaactgt tgatttgatt aagaatagtg
     4981 tcgatagaca tttgatgttc ctcgtaaaaa ctgcttacaa ggaaagaaaa ggaatgcaag
     5041 agacaagcgg tcaaaaaacg gaaatttttt gcaaagaatt agcaaaaaaa caccgcttgt
     5101 atcgtataac ttttctcccc tgtccttttg cctgagcgtt tcacacattg ctgtattttc
     5161 gccttcggcg gcggatttta atccgctctc tccaagggtt cgtccagtaa cagtccccgc
     5221 agtgttcact gcacctgaaa gagtttacct cgtcggtgaa atgttaattc atttctctcc
     5281 agctaccttc atccgaacaa tctaatttat tttataatgt aaaataaaaa agatttgcca
     5341 aattctagca actcttttgc ttccgtcaag tgaatttaac gatatttttg ttctttcggt
     5401 acttcttcta aatcgctaaa taatacggca tatttttggg tggctttatt ggattctaat
     5461 gccactttac acgccatcgt aagtggaaca gaaagtaaca ttcctacggt gccaagtaac
     5521 cacccccaaa atagaagtga gaggaacact acaagggtag aaagcccgag tttcttgccc
     5581 attaatttag gttcaagcgc attacccaca accatattaa tcacgacaat gccgattaaa
     5641 gtgcctaatc ctatagaaaa gccattgagt accaaggctt gaatcacaat tggaattccc
     5701 gccaaaatag agccgatatt cgggacgtag ttaagcagga aggttagaat tgcccataaa
     5761 atcgcatatt ctacgcctag aatttctagt aagatccaaa ctaaccctcc ggtgagtagg
     5821 ctcacaaagg ttttaactcc aaggtaacca atcacccctt caagtacttg gttgatatac
     5881 tctttttcgt tgcttaaatc atcatctggg ctaagtgcga gggctaattt atatttggca
     5941 accggcattt ccaatagcat aaaaatgacg actaagaaaa tcacaaaaat attcgataat
     6001 gcaccggata gccccattag cagtttgctg accaaattca ttattgagct tgggtcaaat
     6061 ttgctcataa tctcttcttg tgagagggta agtggaatat gccatctatg ggcgagttct
     6121 agaatcacct ggattctaga tgaaagctga attttgtatt gcggaatagc attagtaaat
     6181 tcttgcaagg tgttattaat aatgcctgct aagaaactga atcctaagat aaaaacaata
     6241 agtaaaatgg taatggctaa ccataatggc actttacgct tagtcataaa tttgatcatt
     6301 ggtgaacaaa taatggcaat aaatagcgaa agtaaaaatg gcacaacaat ttcattggat
     6361 aatttaatcc ctgccagaat aatgataatg gaggctatcg ccacgagagt tttggtggaa
     6421 tgtgtcataa tttttccttt tacaagcggt taaaaatcag caatttttta caaattagtg
     6481 attctcaaac caactttcta aaattaaaca agcggaaatg gaatccactt tatccttttt
     6541 taatgctttg taaccaccac gagaaaaaat ttccgctttt gcttcaacag tagtgaggcg
     6601 ttcatcttgt agctcaaccg gcagattaaa gcgtccgttt aagcgattgg caaattttct
     6661 ggcacgctga gttaaaggct gctctgtgcc gtccatattc aagggtagcc caacaaccag
     6721 catatctggt ttccaatctt gcaagagttt tccgatctct tcccagtttg gaataccgtc
     6781 ttgggcttta aatgtagtta aaccttgagc tgtgccggta atactttgtc caactgcaca
     6841 gccgatgcta tttgtgccaa aatcaaaggc aatcacggtt tttgttgtgg tttctgtcat
     6901 taagctctcc cagctcttga cggtatgctt gaaagcccca tcatttcata ggcttctgcc
     6961 caacgctcta aataaccggt ttcaaacagc gttttttcat tcgcatcaga cacaatccaa
     7021 taattttgag caatttcttg ctcaagctgg ctgccacgcc aagtggaaca acctaaacac
     7081 acaataaatt tttcaggtga agccagtgtg ccaaaggagt ctaacacatc accggaggta
     7141 gtgagcgtaa ttttatcggt gatcggataa gtgtgaataa aatcattggc ggttttggtg
     7201 tgaataataa agccacgatc ttgatttacc ggcccaccgc ttaacaccat ttggtctttt
     7261 ttgtaatcac gctggttcgc catttggaaa tccattcggg taatcagctc tagaaccgac
     7321 agatcggtag gagtgttaat tactactccc attgcacctt gcggattatg ttcgcaaata
     7381 tagaccaccg tacgctcgaa atagtcatcg tccatttccg gcgtggcaat taaaaattta
     7441 ccttgtaaat tctctaacat taaatatctc caaaacaaac ttgtaacgca gtaattgccg
     7501 caagtgaggc ggtttctgtg cgtaacactc gtttacccag taatacttca gtaaaacctt
     7561 cgctctcggt catggcgatt tcttcggcag ataatccgcc ctcagagcca atcagtaaac
     7621 gaacaccagc tttcggcaca ttcggcagtt cacgaatggt atatttggct cttgggtgca
     7681 aattcagttt caacatatcg tcttgttctt tgcaccagtc ggtgagtttc ataatcgggc
     7741 gaatttcagg gatttcattt cgtccgcatt gttcacaggc ggcaatcgca attttctgcc
     7801 attgctgtaa ttttttatct tgccgttcat cattgagttt cacgccacaa cgttccgacc
     7861 aaagtggggt aatagttttt acccccagct ccaccgattt ttgaatggta aattccattc
     7921 tatcgcctcg ggaaatcact tgccctaaat ggatcggcag gttggattct cggtcatcta
     7981 attcacttga atcaattttt gcaataattt gctttttttc gaccgcttgt aacgtagcgt
     8041 ggaaaacgtg gtttgaacca tcaaataaaa tgatttgctc tccttgtttc attcgtagca
     8101 ctcgcccaac gtggttagcg gcatcttcac ttaacacgca actttgaaga ccagctaacg
     8161 gctgtggttg gtaaattctt gggattctca taattatcta gttataaatt aaaggcagac
     8221 gattgtctgc cataggtaag ttaaaacggg ttaaggataa agtcttgaac aatgcacaat
     8281 agtaataatc gaaatttcat tctctttaac ttcacacact attctatagc cgttatgata
     8341 tagttctctc gtattaataa ttgcaccttt tacaccc
//
LOCUS       DANLXS010000099         8269 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.099, whole genome shotgun sequence.
ACCESSION   DANLXS010000099 DANLXS010000000
VERSION     DANLXS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.099"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            31..1023
                     /locus_tag="PXD71_001695"
     CDS             31..1023
                     /locus_tag="PXD71_001695"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDL6291795.1"
                     /translation="MKKSFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSEVGNGM
                     QDNLSGIEKAVKVNVKCIPSATYEVVHSLAKWKRHTLARFGFKEGEGLFVHMKALRPD
                     EDSLDRTHSVYVDQWDWEKVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSV
                     LPKQITFVHSEDLVQRYPNMTSKERENAICKECGAVFLIGIGGKLSDGKPHDGRAPDY
                     DDWTTESEQGYKGLNGDILVWNEELGTAFELSSMGIRVDETALRLQVALTGDEDRLEM
                     DWHKDLLAGRLPLSIGGGIGQSRLVMFLLRKKHIGEVQSSVWPKAVFEEFENIL"
     gene            complement(1147..1566)
                     /gene="atpC"
                     /locus_tag="PXD71_001696"
     CDS             complement(1147..1566)
                     /gene="atpC"
                     /locus_tag="PXD71_001696"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL6291796.1"
                     /translation="MASQFELTVVSAERKIFEGTVTSVRVSGTDGELGVYAGHAPLLT
                     AIKPGMVKFTLADGKEEFIYVSGGFLEVQPTVVTVLADTAIRGEELDEQRILAAKRKV
                     EETLSKTSDADMTAKLAREIAKLRVYELTKTKLANKR"
     gene            complement(1594..2967)
                     /gene="atpD"
                     /locus_tag="PXD71_001697"
     CDS             complement(1594..2967)
                     /gene="atpD"
                     /locus_tag="PXD71_001697"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL6291797.1"
                     /translation="MATGKIVQIIGAVIDVEFPQDAVPKVYDALKVESGLTLEVQQQL
                     GGGVVRCIALGSSDGLKRGLKVENTNKAIEVPVGTKTLGRIMNVLGEPIDEAGPIGEE
                     ERWTIHRAAPSYEEQANSTELLETGIKVIDLVAPFAKGGKVGLFGGAGVGKTVNMMEL
                     IRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRVA
                     LTGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVL
                     QERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVVLSRNIASLGIYPAVDP
                     LDSTSRQLDPLVVGEEHYNVARGVQGTLQRYKELKDIIAILGMDELSEDDKRLVARAR
                     KIERFLSQPFHVAEVFNGVPGKFVPLKETIRGFKGILEGEYDHIPEQAFYMAGSIDEV
                     VERAKQM"
     gene            complement(2988..3854)
                     /gene="atpG"
                     /locus_tag="PXD71_001698"
     CDS             complement(2988..3854)
                     /gene="atpG"
                     /locus_tag="PXD71_001698"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL6291798.1"
                     /translation="MAGAKEIRTKIASVRNTQKITKAMEMVAASKMRKTQERMAASRP
                     YAESIRKAISHIAKGNIEYKHPFLTPRPVKKVGYLVVSTDRGLCGGLNINLFKTVLHE
                     LKEKDDQGVKSRLAVVGNKGISFFNPMGLEIKGHINGLGDTPAMEDLVGIVNGMVNAY
                     REGEIDEVYVVYNRFINTMSQKPTVQQLLPLPALENDSLEQTGSWDYLYEPNPQALLD
                     SLLVRYLESQVYQAVVDNLASEQAARMVAMKAATDNAGNLINELQLVYNKARQASITN
                     ELNEIVAGAAAI"
     gene            complement(3880..5421)
                     /gene="atpA"
                     /locus_tag="PXD71_001699"
     CDS             complement(3880..5421)
                     /gene="atpA"
                     /locus_tag="PXD71_001699"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL6291799.1"
                     /translation="MQLNSTEISELIKKRIAQFNVVSEAQSTGTIVSVSDGVIRIHGL
                     SDVMQGEMIALPGNRYAIALNLERDSVGAVVMGPYADLAEGMQVQCTGRILEVPVGRG
                     LLGRVVNTLGQPIDGKGEIQNDGFSPIEVIAPGVIDRQSVDQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTIANVVRKLEEHGAL
                     ANTIVVVASASESAALQYLAPYAGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERASRVNADYVERFTNGAVKGQTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESGLFNAGIRPAVNPGISVSRVGGSAQTKLVKKLAGG
                     IRTALAQYRELAAFAQFASDLDEATRKQLSHGQKVTELLKQKQFEPMSVAQLGLSLAA
                     AEYGYLDDIPVERVGSFEANLLAYAQSNYGEFMQELSKSGNFNDEIKDKLNEILSGFK
                     KNSAW"
     gene            complement(5434..5967)
                     /gene="atpH"
                     /locus_tag="PXD71_001700"
     CDS             complement(5434..5967)
                     /gene="atpH"
                     /locus_tag="PXD71_001700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL6291800.1"
                     /translation="MSELSTVARPYAKAAFDFALEQGQLEQWQQMLTFSAAVANDEQV
                     ASFLNSSLASGQISETFIKICGEQLDQYGQNFIRVMADNKRLAVLPAVLNAFLELRAE
                     QESIKDVEVVSADKLTDAQEAKIANAMEKRLGTKVRITSSIDSSLIAGVIIRYDDVVI
                     DGSSRGQLNRLSQELSL"
     gene            complement(5981..6451)
                     /gene="atpF"
                     /locus_tag="PXD71_001701"
     CDS             complement(5981..6451)
                     /gene="atpF"
                     /locus_tag="PXD71_001701"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL6291801.1"
                     /translation="MNLNATLIGQLIAFALFVAFCMKFVWPPLIRVIEERQANIASAL
                     ASAEKAKQEQADSKAAADQEILRAKEEAQNIIDLATKRRNEILESVQAEAEVERQRII
                     EQGYAEVESERKRVQEELRQKVAALAIAGAEKIVGRSVDAAANNDIIDKLVAEL"
     gene            complement(6512..6766)
                     /gene="atpE"
                     /locus_tag="PXD71_001702"
     CDS             complement(6512..6766)
                     /gene="atpE"
                     /locus_tag="PXD71_001702"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDL6291802.1"
                     /translation="MESVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPEL
                     ASSLQTKMFIVAGLLDAIAMIAVGISLLFIFANPFIDLLK"
     gene            complement(6884..7672)
                     /gene="atpB"
                     /locus_tag="PXD71_001703"
     CDS             complement(6884..7672)
                     /gene="atpB"
                     /locus_tag="PXD71_001703"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL6291803.1"
                     /translation="MAGTTAEYISHHLSFLSTGEGFWAVHLDTLFFSLVAGAIFLWVF
                     SRVAKNATTGVPGKLQCFVEIVIEWVNGLVKDNFHGPRNVIAPLALTIFCWVFIMNAI
                     DLIPVDFLPQLANLFGIHYLRAVPTADISATLGMSICVFALILFYTVKSKGFGGLVKE
                     YTLHPFNHWAFIPVNFILETVTLLAKPISLAFRLFGNMYAGELIFILIAVMYMADNFA
                     LQALGIPLHLVWAIFHILVITLQAFIFMMLTIVYLSIAYNKADH"
     gene            complement(7695..8078)
                     /locus_tag="PXD71_001704"
     CDS             complement(7695..8078)
                     /locus_tag="PXD71_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDL6291804.1"
                     /translation="MSAVINRAKSQYKQALKVEFFLLCVFALGVSLWKMNFNFSFLGG
                     ALASFLPHLVFVYWVFFKKSAKNQSKMTAFYRGEGMKWLVTIILVIGCFKLIPALDVV
                     LFFVGYFIALFLNNMIPFVLSKHHH"
BASE COUNT         2461 a         1855 c         1541 g         2412 t
ORIGIN      
        1 atcaacattc tttaattagg aaagcaaaca atgaaaaaat cttttatttt acagcaacaa
       61 gaaatcagtt ttgttaagaa cacatttact caatatttaa ttgataaatt aggcattatt
      121 gaagttcagg ggcctatttt gagcgaagtc ggcaacggta tgcaagacaa cctttccggt
      181 atcgaaaaag cagtaaaagt aaacgtaaaa tgtattccaa gcgctactta tgaagtggtt
      241 cattctcttg caaaatggaa acgccacacc ttagctcgtt ttggatttaa agaaggagaa
      301 gggttatttg ttcatatgaa agctcttcgc cctgatgaag attctttaga tcgcacacac
      361 tcggtatatg ttgaccaatg ggactgggaa aaagtcattc ctgaaggtcg ccgcaacttc
      421 gatttcttaa aagaaacggt aaacgaaatc tataaagcaa ttcgtttaac cgaattagcg
      481 gtggaggctc gtttcgatat tccatctgta ttgcctaaac aaattacttt cgttcacagc
      541 gaagacttag tgcaacgcta tccgaatatg accagcaaag agcgtgaaaa tgctatctgt
      601 aaagagtgcg gagcggtgtt cttaatcggt atcggcggca aattatcaga cggtaaacct
      661 cacgatggac gagctccgga ctatgatgac tggacaaccg agtccgaaca aggctacaaa
      721 ggcttaaacg gcgatatttt agtttggaac gaagaactcg gcacagcctt tgaactttcc
      781 tcaatgggta ttcgtgtaga tgaaaccgcc ttacgcttac aagttgcttt aactggcgac
      841 gaagaccgcc tcgaaatgga ttggcacaaa gatttattag ccggcagact cccgctttct
      901 atcggcggcg gtatcggtca atctcgctta gtgatgttct tactacgcaa aaaacacatc
      961 ggtgaggtgc aatccagtgt ctggccaaaa gcggtatttg aagagtttga gaatattctt
     1021 taataaaaca aaaaagcgaa ccttagggtt cgcttttgct ttctacaagc ggtcttattt
     1081 tgcaaaaaat ttactgtttt taaccgcttg taaaacaaaa ccccactttc gtggggtttt
     1141 ttgatattaa cgtttattcg ctaatttggt tttagtgagt tcgtaaacac gcagtttcgc
     1201 aatttctctc gctaacttcg cggtcatatc cgcatcgctg gtttttgaaa gtgtttcttc
     1261 tactttgcgt tttgcggcaa gaatacgctg ttcgtctagt tcttcaccac gaattgcggt
     1321 atcagccaac actgttacta cggtcggttg tacttcaagg aaaccacctg aaacgtagat
     1381 aaactcttct ttgccgtcag ctaaagtgaa tttaaccatt cccggtttaa ttgcggttaa
     1441 taacggtgca tgccctgcat acacccctaa ttcaccatct gtccctgaaa cacgcacgct
     1501 ggtaactgtg ccttcaaaga ttttacgttc tgcactcact accgtgagtt caaattgaga
     1561 tgccatgttg ttctccttta gtttagaaca aaattacatc tgttttgctc tttcaaccac
     1621 ttcgtcaatt gaacccgcca tatagaacgc ttgttccgga atatggtcgt attcgccttc
     1681 taaaatgcct ttaaagccac gaattgtttc ttttaacggt acgaatttgc ccggtacgcc
     1741 gttgaatact tccgcaacgt ggaatggttg tgaaaggaaa cgttcgattt tacgcgcacg
     1801 agcaacaagg cgtttgtcgt cttccgataa ttcatccata cccaaaattg caatgatgtc
     1861 ttttaactct ttataacgtt gtaatgtacc ttgtacacca cgagcaacgt tatagtgttc
     1921 ctcaccaacc acgagtggat ctaactgacg agaggttgag tcaagagggt caaccgccgg
     1981 gtaaataccg agtgaagcga tattacggct taataccacc gttgagtcta agtgggcaaa
     2041 ggtagttgcc ggagacgggt cagttaagtc atccgccgga acatataccg cttgcacaga
     2101 ggtaatagag ccggttttgg ttgaggtaat acgctcctgt aatacgccca tctcttctgc
     2161 aagagtcggc tgataaccta ccgcagacgg catacgacct aataacgcag atacttcggt
     2221 accagctaaa gtataacggt agatgttatc cacgaagaat aatacgtcac gaccttcatc
     2281 acggaatttt tctgccatgg ttaagccggt taatgccaca cgcagacggt tgcccggtgg
     2341 ttcgttcatt tgaccgtaaa ccagcgatac tttgtctaat acgttagagt ctttcatttc
     2401 gtggtagaag tcattaccct cacgagtacg ctcacctaca ccggcgaata ccgagtaacc
     2461 gctatgttcg atagcgatgt tacggattaa ctccatcata tttacggttt tacctacacc
     2521 cgcaccaccg aataagccaa ctttaccacc cttagcaaat ggagcaacta agtcgataac
     2581 tttgataccg gtctctaaaa gctcggtgct gttcgcttgt tcttcgtagc ttggtgctgc
     2641 acggtggatt gtccaacgtt cttcttcacc gattgggcct gcttcatcaa ttggctcacc
     2701 taatacgttc ataatacgac cgagtgtttt tgtaccaacc ggtacttcaa tcgctttatt
     2761 cgtattttca acttttaagc cacgttttaa accatcagat gaaccaagtg cgatacaacg
     2821 gaccacaccg ccacctaatt gttgttgaac ttcaagggtt aatcctgatt caacttttaa
     2881 tgcatcataa acttttggta ctgcatcttg cggaaactca acatcgatta ccgcaccgat
     2941 gatctgtaca atttttcccg ttgccattac cgttcctcta ttcattgtta aattgctgcg
     3001 gcacccgcga caatttcatt taattcattc gtaatacttg cttgacgagc tttgttatac
     3061 actaactgta actcattaat cagattacct gcgttatcgg ttgctgcttt cattgccacc
     3121 attcgagcag cctgttcaga cgcaagatta tctaccactg cctgataaac ttgagattct
     3181 aaataacgaa ccagtaagct gtctaataac gcttgtggat ttggttcata gagataatcc
     3241 caagaaccag tttgctctaa tgagtcattt tccagtgcag gcaaaggaag caactgttgt
     3301 actgtcggtt tttgtgacat cgtgtttata aaacggttat ataccacata cacttcatca
     3361 atttcgcctt cacggtaggc atttaccata ccattaacaa taccgactaa atcttccatt
     3421 gccggtgtat cacccaatcc attgatatga cctttaatct ctagccccat tgggttaaaa
     3481 aaggagatcc ctttatttcc caccacagca agtcgagact taacaccttg gtcatctttt
     3541 tctttcaatt catgtaaaac ggttttaaat aaattgatat ttaagccacc acataaaccg
     3601 cgatcggttg aaactactaa atagccaact ttttttaccg gacgtggggt caaaaatggg
     3661 tgtttatact caatgttacc tttggcaata tggctaattg ccttgcgaat actttcagca
     3721 taagggcgag aagccgccat acgctcttgg gttttacgca tttttgatgc ggcaaccatt
     3781 tccatcgctt tggtaatttt ttgtgtatta cgaacacttg caattttggt tcttatctct
     3841 ttagcacctg ccatattatt tctccgctaa aaagttagat taccaagcac tgttcttttt
     3901 gaaaccagat aaaatctcgt tcaatttatc tttaatttca tcgttgaaat tgccggattt
     3961 tgacagttct tgcataaact cgccgtagtt actttgagca taagctaata agtttgcttc
     4021 aaatgagcca acacgctcaa ccggaatatc atctaagtaa ccgtattcag ctgctgctaa
     4081 tgataaacct aattgtgcaa cagacatcgg ctcgaattgt ttttgtttca acaattcagt
     4141 tactttttga ccgtgtgaaa gctgtttacg tgttgcttca tcaagatctg atgcgaactg
     4201 ggcaaatgcc gctaattcac ggtattgagc aagtgcggta cggataccac cggcaagctt
     4261 cttaaccagt ttagtttgtg cagaaccacc tacacgggaa accgagatac ccgggtttac
     4321 cgcagggcga ataccggcat tgaataagcc tgattctaag aaaatctgac catcggtaat
     4381 tgaaattacg ttagtcggta cgaaggctga aacgtcacct gcttgggttt cgatgatcgg
     4441 taatgcggtt aaagaaccgg tttgaccttt cacagcaccg ttagtgaaac gttctacata
     4501 gtctgcattt acacgagacg cacgctctaa taagcgagag tgtagataga acacatcacc
     4561 cgggaatgct tcacgacccg gtggacggcg taataacaat gaaatttgac ggtaagcaac
     4621 tgcttgctta gacaaatcat cgtaaacgat caacgcatct tcaccacggt cacggaagta
     4681 ttcacccatt gcacaacctg cataaggtgc aagatattgt aatgcagcag attcagacgc
     4741 agaagccact acaacgatag tgttcgctaa tgcaccgtgt tcttctaatt tacgcaccac
     4801 gttggcgata gttgatgcct tttgaccgat tgcaacgtag atacatttaa tgcctgaatc
     4861 acgttggttg atgatcgcat caatcgctaa tgcggtttta ccggtttgac ggtcacctat
     4921 aatcaattca cgctgaccac gaccgattgg taccattgag tccaccgctt tgtaaccggt
     4981 ttgaaccggt tgatcaaccg attgacggtc gataacgccc ggtgcgatca cttcgattgg
     5041 tgagaaaccg tcattttgga tttcgccttt accatcgatt ggctgaccta aggtatttac
     5101 aacacgacct aataagccac gaccaaccgg cacttctaaa atacgaccgg tacattgtac
     5161 ttgcatacct tctgctaaat cggcataagg tcccattaca actgcaccaa cagaatctct
     5221 ttctaagttt aatgcaatcg cataacgatt gcccggtaat gcaatcattt caccttgcat
     5281 tacatcagat aagccgtgga tacgaataac cccgtcactt accgaaacga ttgtccctgt
     5341 gctttgagct tcactcacca cattaaactg ggcaatacgt tttttaatca attcactaat
     5401 ttcagttgaa tttagttgca ttttttattc ctcttacaag ctcaactctt gactcaagcg
     5461 gtttagctgt ccacgactac taccatcaat cactacatca tcataacgaa tgatcacacc
     5521 cgcaattaat gaactatcta ttgatgaagt aatacgtact ttcgttccga gtcgcttttc
     5581 catcgcattg gcaatttttg cttcttgtgc atctgttaat ttatctgcag aaacaacctc
     5641 aacatcttta attgattcct gttctgctct taattctaaa aatgcattca ataccgcagg
     5701 taatactgct aaacgcttgt tatcagccat tacgcgaatg aaattttgcc catattgatc
     5761 taattgttcg ccacaaatct tgataaacgt ctcagaaatc tgaccgcttg caagtgatga
     5821 gttaagaaaa ctagctactt gctcatcgtt agcgacggcg gcagaaaaag ttaacatctg
     5881 ctgccattgt tccaattgac cttgttctaa agcaaaatcg aaagccgctt tagcataggg
     5941 gcgagctact gtgcttaatt ctgacatctg cccctcctag ttatagttcc gcaactagct
     6001 tatcaataat atcgttgttc gcagcggcat ccaccgaacg acccacgatt ttttctgcac
     6061 cagcaatagc caatgcagcc actttttgac gaagctcttc ttgaacgcgt ttacgttcgc
     6121 tttcaacttc cgcgtaaccc tgttcaataa tacgttgacg ttccacttca gcttctgctt
     6181 gtacagattc taaaatttca ttacgacgtt ttgttgctaa atcaatgata ttttgtgctt
     6241 cttctttcgc tctaaggatt tcttgatccg cagccgcttt agaatcagct tgttcttgtt
     6301 tcgctttttc ggcagaagcc aatgcgctag caatatttgc ctgacgctct tctatcactc
     6361 gaattagcgg tggccataca aacttcatac agaacgcaac aaaaagtgcg aacgcaatca
     6421 gttgaccaat tagtgttgca tttaaattca caacgccctc ctaaaagccg gtaaagttat
     6481 tcaactgaat aacggttaat caaatcccga cttatttcaa taaatcaatg aacgggtttg
     6541 cgaagatgaa tagtaatgaa atacctacag cgatcattgc gatagcatct aaaagaccag
     6601 caacgataaa cattttagtt tgtaagcttg atgctaattc cggctgacgt gcagatgact
     6661 ctaagaattt gccacctaag atagcaaagc cgatagcagt accaagagca gcgaaagcta
     6721 ataaaattga agcaccaata attgttgctg tgattactga ttccataatg ttctccatta
     6781 gattgaggaa ttagcccgaa ggctgggttg ttgataaaaa tacatatcaa gcggtcaata
     6841 ttttgcaaaa atttgcgaaa aacctaccgc ttgtaaggct agtttaatga tccgctttgt
     6901 tataagcgat actcaaataa acaatcgtta gcatcataaa aataaaggct tgaagcgtaa
     6961 taactaaaat atggaaaatc gcccaaacca agtgtaatgg aatccccaac gcctgtagag
     7021 cgaagttgtc tgccatatac attactgcaa taaggataaa gattaactca cctgcataca
     7081 tattaccgaa taaacggaac gccaatgaaa tcggttttgc caaaagcgtt accgtttcaa
     7141 gaataaagtt taccggaatg aatgcccagt ggttaaacgg gtgtaacgtg tattctttca
     7201 ctaaaccgcc aaaacctttc gatttcaccg tgtaaaataa aattaacgca aatacacaaa
     7261 tagacatacc cagtgttgca ctaatatctg ccgttggtac tgcacgcaaa tagtgaatac
     7321 caaacagatt ggctaactgt ggtaagaaat cgaccgggat aaggtcgatc gcattcataa
     7381 taaacaccca acagaaaatc gttaatgcta atggtgcaat tacattgcga ggtccgtgga
     7441 agttatcctt cactaaaccg tttacccact caataacaat ctcaacaaaa cattgcaatt
     7501 ttcccggaac gcccgttgtt gcattttttg ctacacgcga gaacacccat aaaaaaatcg
     7561 cacctgccac taaagagaag aacagtgtat ctaaatgcac tgcccaaaag ccttcacctg
     7621 tcgataaaaa actcaagtga tggctaatat attctgctgt cgttccagcc ataacatatc
     7681 cttttaaatt aaaattagtg gtgatgtttg cttaatacaa acgggatcat attatttaaa
     7741 aataatgcga taaaataacc cacgaaaaac aacacaacat ctagagcagg gataagttta
     7801 aaacagccga tcaccaaaat aatagtaacc aaccatttca ttccttcacc acgatagaaa
     7861 gcggtcattt ttgactgatt ttttgcagat ttcttaaaga aaacccaata tacaaacaca
     7921 agatgaggca aaaaactcgc taaagcaccg cctagaaagg aaaaattaaa attcattttc
     7981 cataaagaca ctccaagtgc aaaaacgcac aagagaaaaa attcaacctt taatgcttgt
     8041 ttatattgtg attttgctct attgattaca gctgacacat tttatccttt ttgaataagt
     8101 aaaccacaca atcaggctaa taattagcca ctgttgaatc aaagaaaatc cgtgctaaat
     8161 tatactttac aaatgaattg ttgcaaccga aaagcgacac aaaaacttgt tattcttcac
     8221 atttttgaaa aaaaatttta tttacgttgc atttttttga gtaaaacca
//
LOCUS       DANLXS010000100         8251 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.100, whole genome shotgun sequence.
ACCESSION   DANLXS010000100 DANLXS010000000
VERSION     DANLXS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.100"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            55..2442
                     /locus_tag="PXD71_001705"
     CDS             55..2442
                     /locus_tag="PXD71_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDL6291805.1"
                     /translation="MEKMMRKINQLIISPYFFLSILSASANSDFWKSDLNFNLTNITK
                     RVGVDNFTTPVAGEPWAGIGPNGEAAGTAPLYYSRIPAMQVTEDNKLVVMFDLRWNRA
                     YDQDRIDPGIAISSDGGHTWTKKTAWSFDRTNHPARRSMDPTLLHNPIDNSLYVMHGT
                     WSMSDQQWYGGRVPHFNSGSWAATIYKSTDGGLNWEKNTEFSKFSNLDVFSKVTKNGK
                     PTLGFLGGVGSGIVMQDGTLVFPIQTAHQNGIATTIMYSKDNGKTWEMPEIDNPVAPN
                     QSSLENMVFELEPGKLVMTGRGNSRWAYSSTDMGKTWELFTPTNGLLPTSSQPTQGSS
                     IYVTLPNGRKVLLISKPDGKNDGWKRGDITLWMLDAKDPSHKHKVHIIRPGSGNAAGA
                     GYSSLAYKEGNLFVAFENDGDISVKNLTEHIAEMEAKAIEWGLPDELTPALDKINALP
                     YLNKAQKETLVEKMRRANDTAIAQSFVINKEMYNLKNNSDELDKLAKNITKALPSQQN
                     IYQRALAYVNKVTNTADTTYLNYNGIMDTYANLYESYLALNTKLDFTRYIQKARKFNE
                     YNTDILYRSFDNFFAHYDTGVKHNNLSLGLNTKLSENLRGGLFFEYSNKNRNSVQIGA
                     RAKYEMDNHQLSGFLRYRGVKHKDMLARNNSVDGYLNYAYRIQLDDKLSISPSVGAYV
                     SRSSRSLIDEDLAINSRTVYASDVGLNIHYKLNDIDAYIRPSIGFVNGDITLSQSNDM
                     TNTYKIKDKSNVYSVTTGLKKRFANGVALGADFKLHRYGSQTTESNFGLNVSYNW"
     gene            complement(2609..3145)
                     /gene="hpt"
                     /locus_tag="PXD71_001706"
     CDS             complement(2609..3145)
                     /gene="hpt"
                     /locus_tag="PXD71_001706"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL6291806.1"
                     /translation="MEKHHIETLHSKEEVLNRINELAKEINQHYHQKQCQNLVVVGLL
                     RGSFMFMADLVRKLELPVEVDFLTASSYGSSTESSRDVKILKDLDGEIQGKDVLIVED
                     IIDTGFTLSKVGEILKLREPNSLAICTLLDKPCRREVDVPVKWVGFEIPDEFVVGYGI
                     DYAQKYRHLDYIAKVVIE"
     gene            complement(3184..4533)
                     /gene="pmbA"
                     /locus_tag="PXD71_001707"
     CDS             complement(3184..4533)
                     /gene="pmbA"
                     /locus_tag="PXD71_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDL6291807.1"
                     /translation="MSITDKQTLLQQEQELRQAVEFALNFAKQAGAEAEVGVTKVAGL
                     SVSSRLEQVENIEFNNDGSLGISVYLGKRKGNASTSDLQPESIQKTVEAALAIAKYTS
                     EDACTGLADKELLAFEAEDLELYHQADISVDQAVELAIEAEHYALNADEKIVNSDGAT
                     FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTIFREFDKLQAPK
                     WVGEQAAIKAVDRLNPQRIKTGEMSVIFYNDVATGLVGHLAGAISGGALYRKSSFLLD
                     KLGEQILPEWFAISERPHLLRQLASSAFDSEGVITQNREIITDGVLQTYLMTSYSGRK
                     MGLKSTGHAGGIHNWLVKPNRQGGLNTLLKEMGTGLLVTEMLGSAINSVTGEYSRGAA
                     GFWVENGEIQYPVAEITIAGQLQDMYKNLVAVGDDIEHRSNIQTGSILLEKMMVSGE"
     gene            4632..5162
                     /locus_tag="PXD71_001708"
     CDS             4632..5162
                     /locus_tag="PXD71_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDL6291808.1"
                     /translation="MAKKRSKNEIDWTDEEEEIIWVSKSEIKRDSEHLKKIGAELIEL
                     TPQNLEKIPLDDDLKEAIRQAQGFKMEARRRQIQFIGKLLRNRDQEPIQEALDKVKNR
                     HNQQQALLHKLELVRNQLIAMGDASLDNLLTEYPQLDRQHLRNLIRGAIKEREANKPA
                     KNYREIYQYLKTEIVE"
     gene            5168..5539
                     /locus_tag="PXD71_001709"
     CDS             5168..5539
                     /locus_tag="PXD71_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HDL6291809.1"
                     /translation="MEYLSTILKAHIGLAYISLILLLTRGFLSSKMVDWRQYKILRIA
                     PHAVDTFLLITGIAAVAIFLAYDFFTLAQFSWLLPKLLFLVLYIVFATKAFKKGQPFS
                     LKFYLLAVVSFLLMMLTATFH"
     gene            5552..6265
                     /locus_tag="PXD71_001710"
     CDS             5552..6265
                     /locus_tag="PXD71_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL6291810.1"
                     /translation="MIGPFVNAGAIILGGVLGACLGSHLPERIRTTLPLVFGLCCFGL
                     GMMLTASVKYMSAVALALILGTIIGELIYLENRIGKVAGLARGLIDRLFPPVHSLSQE
                     EFLEKFIALLVLFCVSGTGIFGAIKEGMTGDPSILYIKSILDFFTAMIFAASLGYAIA
                     AIAVPLIIVQGILILLATVIMPLTTANMMADFSATGGLLLFATGFRICGIKNFPVANM
                     LPALLLAMPMSYFWEWLVH"
     gene            6268..6831
                     /locus_tag="PXD71_001711"
     CDS             6268..6831
                     /locus_tag="PXD71_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL6291811.1"
                     /translation="MKKRIIVGISGASGFQYGYKVLELLKPFSEIETHLVISKGAELT
                     RTLETDYTREALHDLADQIHSLNNVGASIASGSFKTEGMVIAPCSMRTLAAIANGLSD
                     NLLTRAADVVLKERRKLVLMVREAPLNLAHIDNMRKVTEMGGIIFPPIPAFYQKPTSL
                     DEMVTHSVSHALSLLDIDIPNTPHWGE"
     gene            6894..7334
                     /locus_tag="PXD71_001712"
     CDS             6894..7334
                     /locus_tag="PXD71_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDL6291812.1"
                     /translation="MHNPIPTHILDFIQTNHVVNIAAHTEQDFWAANCFYAFDQENVR
                     LIILTAKKTRHAGLMLENPHIVGTICGQIEKINEIEGVQFSATAECLEDKQAEKAALQ
                     IYYQKHPLARLKPSDVWVLSFNTIKHTGNKLMFAKKTIWERNII"
     gene            complement(7338..8213)
                     /locus_tag="PXD71_001713"
     CDS             complement(7338..8213)
                     /locus_tag="PXD71_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6291813.1"
                     /translation="MMKTILIDEANQIKGFSHLYRLRSDLMLKYTEISVTETVEQEPA
                     EFEAGLRLVILLSGRSEIDFNSEVFELDASLKPQAAFLPLNRAEMGRKIFRKGQLQKE
                     LVIFLYPKWLIESQLNLASYHHFKQHLKAHQIILTQSMLKLIHDIVSEQEMQPFGLLE
                     KEGNLLALLASAFSQLTQAPRLKKDRVQQLTELLESGKFDGWSLKQIAIYFHTNVTTL
                     QNEFQHYHKTSIFAYLRRSKLEQAYKLLLQGQSITQAAEFAGYANSENFSTAFKRTFG
                     ITPSQIKRDKAKGII"
BASE COUNT         2481 a         1648 c         1554 g         2568 t
ORIGIN      
        1 gaaccatata atagctgttc agctaaaaat tagtctttgc actttatccc cctaatggag
       61 aaaatgatga gaaaaatcaa tcagttaatc atatccccat actttttcct aagtatttta
      121 tctgcttccg ctaactctga tttttggaaa agcgacctta attttaatct cacaaatatt
      181 accaagcgtg tcggagtcga taactttacc actccagtag ctggcgagcc ttgggctggt
      241 attggtccaa atggtgaagc tgcaggtact gcgccattat attatagccg tattccagct
      301 atgcaagtta ccgaagataa caaattagtt gttatgtttg atttacgctg gaatcgtgcg
      361 tatgaccaag atcgtattga tcctggtatt gcgatttctt ccgatggtgg ccatacttgg
      421 acgaagaaaa ccgcttggtc ttttgataga actaatcacc cagcacgccg ttcaatggat
      481 ccaacgctat tacataatcc tattgataat agtttatatg taatgcacgg aacttggtct
      541 atgtcggatc aacaatggta tggtgggcgt gttcctcact ttaatagtgg cagctgggca
      601 gctaccattt ataaatctac tgatggtggt ttaaattggg agaaaaatac tgagtttagt
      661 aaattcagca atttagacgt attttctaaa gtcaccaaaa atggaaaacc taccttaggc
      721 tttttaggcg gggtcggatc tggaattgta atgcaagatg gtacgttggt tttcccaatt
      781 caaaccgcac atcaaaatgg tattgcgacc actattatgt attctaagga taatgggaaa
      841 acttgggaaa tgccagaaat tgacaaccca gttgcaccga accaaagttc tttagaaaat
      901 atggtgtttg aactcgaacc gggtaagcta gtgatgactg gacgaggaaa cagtcgctgg
      961 gcatactctt ctactgatat gggtaagacc tgggaattat ttactccaac taatggatta
     1021 ttaccaacta gctcacaacc aactcaaggc tcttctatct atgtaacact accaaatggt
     1081 cgaaaagtgc tactaatctc taaaccagat ggaaagaatg atgggtggaa aaggggtgat
     1141 attacacttt ggatgctaga tgcaaaagat ccatcgcaca aacacaaggt gcatattatt
     1201 cgtccaggtt caggtaatgc cgctggtgct ggttattcct ctttagccta taaagaaggg
     1261 aatttatttg ttgcttttga aaacgatggt gatatttcag taaaaaattt aactgaacat
     1321 attgctgaaa tggaagcaaa agctatcgaa tggggattac cagatgaact tactcctgct
     1381 ttggataaaa tcaatgcact gccttatctc aataaagctc aaaaagagac gctagtcgaa
     1441 aaaatgcgtc gtgctaatga tactgcgatt gcacaatctt ttgtgattaa caaagaaatg
     1501 tataatctga aaaacaatag tgatgagctg gataaattgg caaaaaatat tactaaagca
     1561 ctgccgtctc aacagaatat ttatcaaaga gccttagcct atgtgaataa agtcaccaat
     1621 acagcggata ccacttattt aaattataat ggtattatgg atacctatgc taatttatat
     1681 gaaagctatt tggctttaaa tactaaatta gacttcactc gctacatcca aaaagcgaga
     1741 aagttcaatg aatataacac tgatatttta tatcgtagct ttgataattt ctttgcccat
     1801 tacgacacag gtgtaaaaca caataatcta tccttaggct tgaatacaaa attaagtgag
     1861 aatttacgtg gtggcttatt ctttgaatac agtaataaaa atcgtaatag cgtacaaatt
     1921 ggtgcaagag ctaaatatga aatggataat caccaacttt ctggcttctt acgctatcgt
     1981 ggcgtaaaac ataaagatat gctagcacgt aataacagtg ttgatggcta cctaaattat
     2041 gcttatcgta ttcaattaga tgataaatta agcattagcc catcagttgg tgcttacgtt
     2101 tcacgttctt ctcgctcatt aatagatgaa gatcttgcga ttaatagccg cacggtctat
     2161 gcaagtgatg taggattgaa tatccattat aagctaaatg atattgatgc ttatattcgt
     2221 ccaagtatag gctttgtcaa tggtgatata actttatctc aatctaatga tatgacaaac
     2281 acatacaaaa taaaggataa aagtaacgtg tattctgtca cgacagggtt gaaaaaacgc
     2341 tttgcaaatg gtgtggcatt aggggctgat ttcaaattac atcgttatgg ttctcaaact
     2401 actgaaagta atttcggtct aaatgtaagt tataactggt aataccgtaa actgattacg
     2461 atttaaaagt tagactagcc caagactgag ttctatattc catagagctc agtcttttaa
     2521 tttttctttc tcgataagaa tattttatat ttcaacctta atcgagaatg taattagtcg
     2581 tgaaaatcta agtagtgatt gagttttttt attcaatcac tactttagca atataatcta
     2641 aatgacgata tttttgggca taatcaatcc cataacccac cacaaattca tcggggatct
     2701 caaatcccac ccattttacg ggcacatcaa cttcgcggcg gcaaggttta tctaataaag
     2761 tacaaatagc aagtgaattt ggctcacgta acttgagaat ctcccctacc ttgcttaagg
     2821 tgaagccagt atcaataata tcttctacaa tcaatacatc tttaccttga atttcgccat
     2881 ctaaatcttt tagaattttg acatcacggc tggattccgt gctgctgcca tagcttgatg
     2941 cggttagaaa atccacttct acaggtaact ctagctttct tactaaatct gccataaaca
     3001 taaacgagcc tcgcaataaa ccaaccacta caaggttttg gcactgtttt tggtgatagt
     3061 gttgattgat ttcttttgct aattcattga ttcggtttaa tacttcttct tttgaatgta
     3121 gcgtttcgat atgatgtttt tccattgatt tctccaaata aaattgatta caagcggccg
     3181 tttttactcg cctgaaacca tcattttttc taataagatt gaacctgttt ggatatttga
     3241 gcggtgttca atatcatcgc ctaccgccac taaattttta tacatatctt gcagttgccc
     3301 tgcaatggta atttccgcta cagggtattg aatttcaccg ttttctaccc aaaatcctgc
     3361 tgcacctcga gagtattccc ctgttacgct attaatcgca gaacctagca tttccgttac
     3421 taataatcct gttcccattt ccttaagtaa cgtatttaaa ccaccttgac gatttggttt
     3481 aactaaccag ttatgaatac cacctgcgtg tcctgtactt tttaacccca ttttacgccc
     3541 tgaatagcta gtcattaaat acgtttgtag cacgccatca gtaataattt cacggttttg
     3601 ggtaattaca ccttcactat caaacgcaga agaggctaat tgacgcaaca aatgcggacg
     3661 ctctgaaatc gcaaaccatt caggcagaat ctgttcgcct aatttatcta ataagaaact
     3721 tgatttgcgg tataatgcac cgccactaat agctccggcc aaatgcccca ctaaaccggt
     3781 tgccacatcg ttatagaaaa tcacagacat ttcgccggtc ttaattcgtt gcggattcaa
     3841 tctatctacc gctttaattg cagcttgctc ccctacccat ttcggtgctt gtaatttatc
     3901 aaactcacgg aaaatggtgt attcataatc acgctcaagc tgatcttcat aagctgaaat
     3961 cacgctacaa gagagtgaat atcggctcga caggtaactc tgcaacattc cgtgcgtatt
     4021 gccataaaca cgcacgccac tgtgagaatt gaaagttgcc ccgtcactat tcacaatttt
     4081 ttcgtctgca tttaaggcat aatgctccgc ttcaatcgcc aattcgactg cttgatcaac
     4141 tgaaatatcc gcttggtggt acaattccaa atcttctgcc tcaaaggcaa gcaattcttt
     4201 atcagcaagc ccggtacaag catcttctga ggtatatttt gcaatcgcaa gagctgcttc
     4261 aaccgttttt tgaatcgact ctggctgaag atcggaggtg gaagcattac ctttgcgttt
     4321 gcctaaataa accgaaatac cgagagaacc atcgttattg aactcgatgt tttcaacttg
     4381 ctctaaacgg cttgaaaccg aaagcccggc aactttagtc acgcctactt ccgcctctgc
     4441 cccagcctgt tttgcaaaat ttaaggcaaa ttcgaccgct tgtcgtagct cctgttcttg
     4501 ttgtaacaag gtttgtttat ctgtaattga cattttgttc cattgattga ttgcattgat
     4561 gaatatattc taactcaaat ttctgttaga atacggcatt ttttagagtt tatgtaatga
     4621 aaggataaag aatggctaaa aaacggagca aaaacgaaat tgactggaca gatgaggaag
     4681 aagaaatcat ctgggtcagc aaaagtgaga taaaacgaga ttctgaacac ttaaaaaaaa
     4741 ttggggctga attgatcgaa ttgaccccac aaaatttgga aaaaattcca ctagacgatg
     4801 atttaaaaga ggcaattcgc caagctcaag gctttaaaat ggaggctcgc cgccgtcaaa
     4861 ttcaatttat cggcaaatta ttacgcaacc gtgaccagga gccgattcaa gaagccttag
     4921 ataaagtgaa aaatcgccat aatcagcaac aagccctatt acataaattg gaattagtgc
     4981 gtaatcaact gattgcaatg ggcgatgcat cattagataa tttattgacg gaatatcctc
     5041 aattagaccg ccaacatttg cgtaatttaa ttcgtggggc aattaaagag cgtgaagcca
     5101 ataaaccggc aaaaaattac cgtgaaattt atcaatattt aaaaactgaa atcgtggagt
     5161 gattattatg gagtacctat caaccattct taaagctcat atcggcttag cttatattag
     5221 tttaatttta ttattaaccc gtggattttt atcgtcaaag atggtggatt ggcgacaata
     5281 taaaattttg cgcattgccc cacacgcagt tgataccttc ttacttatca ccggtattgc
     5341 tgctgtcgct attttcttag cttatgattt tttcacctta gcacaattca gttggttgct
     5401 accaaagtta ctcttcttag tactttatat tgtatttgcg acaaaagcgt tcaaaaaagg
     5461 acaaccgttt tcgctcaaat tttatctgtt agccgtagtc tcattcttat taatgatgct
     5521 aactgccact tttcactaaa taagggaaac tatgatcggg ccttttgtga atgccggtgc
     5581 aattatttta gggggcgtgc tgggtgcttg tttagggagt cacctgcctg aacgtattcg
     5641 cactacgttg ccgctggtat tcggcttatg ctgtttcggc ttaggtatga tgctgactgc
     5701 ctcagtaaaa tatatgtctg cggttgcctt agcattgatt ttaggcacga tcatcggtga
     5761 actgatttat ttagaaaaca gaatcggtaa agttgccggt ttagctcgtg gtttaattga
     5821 ccgcctattc ccaccggtgc atagtctttc ccaagaagag tttttagaga aatttattgc
     5881 tctgcttgta ctgttttgtg tgagcggtac cggcattttt ggtgcaataa aggaaggaat
     5941 gacaggcgat ccaagtattc tttacattaa atcgattcta gatttcttca ccgcaatgat
     6001 tttcgctgct tcgttaggct atgccattgc ggcgattgca gtgccgttga ttattgtgca
     6061 agggatttta attctactgg caaccgtgat tatgccttta accactgcca atatgatggc
     6121 agatttctcc gcaaccggcg gtttactgct atttgcaacc ggctttcgca tttgtggaat
     6181 taaaaacttt ccggtcgcaa atatgctacc agcactactc cttgcgatgc cgatgtctta
     6241 tttctgggaa tggttggtac attaatgatg aaaaaacgta ttattgtcgg cattagtggg
     6301 gcaagtggtt ttcaatatgg ctataaagtg cttgaactgc tgaaaccgtt ttcagaaatt
     6361 gaaacccacc ttgtaatcag caaaggagct gaactcactc gtactctcga aacggattat
     6421 acccgagaag ctcttcatga tttagctgat caaatacatt cgctaaacaa tgtcggagca
     6481 agcattgcca gtggttcatt taaaaccgaa ggtatggtca tcgccccgtg ttctatgcga
     6541 acccttgccg ctattgccaa cggtttgagc gataatttgc tgacccgtgc ggcagatgtg
     6601 gtgttgaaag aacggcgtaa attggtatta atggtgagag aagctccgct caaccttgct
     6661 catattgaca atatgcgaaa agtcacagaa atgggcggca ttattttccc acccattccg
     6721 gcgttttacc aaaagccaac cagccttgac gagatggtca cccacagcgt aagccatgcc
     6781 ctttccctgt tggatattga cattccaaat accccacatt ggggagaata attcggctta
     6841 tcttttgctt tttgaaatgc tagaatacct gataaaaact atcaggtatt tttatgcaca
     6901 atccaatccc gacccatatt ttagatttca ttcaaactaa ccacgttgtc aatattgcag
     6961 ctcataccga acaggatttt tgggcagcaa attgctttta tgcctttgac caagaaaatg
     7021 ttcgcttgat tatcttaacc gccaagaaaa ccagacacgc tggcttgatg cttgaaaacc
     7081 cacatattgt cggcacaatt tgtgggcaaa ttgagaagat caacgaaatt gaaggtgtcc
     7141 aattctccgc taccgccgaa tgtttagaag ataaacaagc cgaaaaagcc gctttgcaaa
     7201 tttattacca aaaacacccg cttgcacggc ttaagcctag cgatgtttgg gtgttatcat
     7261 tcaataccat caagcacacc ggtaataaac ttatgtttgc gaaaaagacg atttgggaga
     7321 gaaatataat ttgaagctca aataatgcct ttagccttat cccgtttgat ttggcttggt
     7381 gttatcccaa aagttcgctt aaatgccgtt gaaaaattct ccgaattggc atatccggca
     7441 aattcagctg cttgtgtaat actctgccct tgtaatagta atttataggc ttgttctaat
     7501 ttagaacgcc ttaaatacgc aaaaatagag gttttgtggt aatgctgaaa ttcattttgt
     7561 aatgtggtca cattggtatg gaaatatatt gcaatctgct tcaaactcca gccgtcaaat
     7621 ttaccgctct caagcaattc tgttaactgt tgcacccgat ctttttttaa ccggggagcc
     7681 tgtgtcagct gagaaaaagc acttgctaat aaagctaata gattaccttc tttctcaagt
     7741 aacccaaatg gttgcatttc ctgctctgac acaatatcgt gaataagttt taacatactc
     7801 tgagtcaaaa taatttggtg ggcttttaaa tgctgtttaa agtgatgata gcttgccaaa
     7861 tttaactggc tttctattag ccatttaggg tagagaaaga taacaagctc tttttgaagt
     7921 tgccctttcc taaaaatttt acgccccatt tcagctctat ttagcggtaa aaatgcggct
     7981 tgaggtttta aacttgcatc taattcaaat acttccgaat taaaatcaat ttccgatcga
     8041 ccactcaaca atataaccaa tcttagtccg gcttcaaatt ccgcaggttc ttgctctacg
     8101 gtttcagtaa ctgaaatctc tgtatatttc agcataagat ctgaacgtag tcggtataaa
     8161 tgtgaaaaac cttttatttg gttagcttca tcaatcaaga ttgttttcat cattaaaaac
     8221 tttttttcgc atagtcaaaa ttttatttta c
//
LOCUS       DANLXS010000101         8184 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.101, whole genome shotgun sequence.
ACCESSION   DANLXS010000101 DANLXS010000000
VERSION     DANLXS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.101"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            34..948
                     /locus_tag="PXD71_001714"
     CDS             34..948
                     /locus_tag="PXD71_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6291814.1"
                     /translation="MPSSYQLVLINLLYIFGIGLNIVLLRFLSLHFDPLNNNGIRFLV
                     GGFTLLFFILWRFRTSLSQLIKTPKLIAISLFVGLMMSANMYFWLKGVKITNAVTVSI
                     FGVLAMPFGVIIAAIFFQDERERIKSKAFWFGSILTIIGSLGFIWHGKTLSIGEGFIL
                     GSVFLFLSIIIRNIQNLVVKFARELNVLTLSCVTSLTTSLSSLLLSQQAGKFNELFTA
                     PNWLLFSLMVAGIYAIMIGMVLTFHIIQTQGLITYQVLELLMPVSTAIIAYILLDERI
                     SIVQMGFACIVILGSSIALKLVKYPKHY"
     gene            1067..1927
                     /gene="araC"
                     /locus_tag="PXD71_001715"
     CDS             1067..1927
                     /gene="araC"
                     /locus_tag="PXD71_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDL6291815.1"
                     /translation="MYKNLSLLSPTYPFDLEFIAGLTQCEKGNYLDSIVDRQQGMHGY
                     MLQLTTFGRGSVFDGKHVFTAHRGQLLLFTPNAVQHYHRHPESQYWHYKWVYFQPNPK
                     WNKWLNWSDMEEKIGRITISDDRYFQEISQLFSKIELELKSSKFFKEDIATSLLEYLL
                     MKCISAEKIEVIPVIDQRILTVCDLILTNLAKNESLEAMAEKVFLSPSRLSHLFKQSL
                     GISLVQWRELQRISESKKLLYFSNISISNIAKSLGYEDSLYFSKIFKKHTGLSPSEFR
                     TLERRKDSEM"
     gene            2114..3088
                     /locus_tag="PXD71_001716"
     CDS             2114..3088
                     /locus_tag="PXD71_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL6291816.1"
                     /translation="MKKPTFKHALFSSILALACYGSAFANDVVNISKIDGMPWFNRMA
                     EGVVQAGKDNNINAYQVGPSNTDAPQQVKLIEDLIAKKVSAISIVPNDANALEPVLKK
                     AKQNGIVVLTNESVGQPSADWDIEIIDNAQFAADYVEEVAKAMGGKGGYVIYVGGLTV
                     PQHNLWADLFVKYQKEHYPDMFEVTSRMPVAENINDARRTTIDLVKAHSDLKAVVSFG
                     SQGPIGAGLAVKEKRLKNKLNVFGMMIPSQAANLIKSGDITMGITYDPANAGYALAAV
                     AAKVLKGEKIEDGLEIPTVGKAKVDSEKKLLQFHNVLKVTKENIDKLY"
     gene            3143..4633
                     /locus_tag="PXD71_001717"
     CDS             3143..4633
                     /locus_tag="PXD71_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6291817.1"
                     /translation="MAFISMRHISKTFHGIKALNQVNLSLDYGEALCLAGQNGCGKST
                     LIKILSGVYQPDKGAEIQIGATQYTKLTPQESIDKGIQVIYQDLALFPNLTVAENIAI
                     NLHRKFGWVSQDHIHQVALTAIQSINANLDLNAILEDLPIAQQQLVAICRALAQNARL
                     LIMDEPTASLTAKEVKDLLDVVIKLKSKGISIIFVSHKLQEVMSVSDSVLVLKDGNMV
                     GQYPIREMNEKRLGFLMTGLEIDYKRLDLPDFLQRKTVLEVRNLTLPHQYQEINFSLR
                     EGEIVALTGLLGSGRTELCLSLFGITQPQSGDILLNGEKVIFNTNRDAIAKGIAYVSE
                     DRMHTGLIMAESIHHNIISTIFHKITDKLQIIQSSKAYASSQALIDSLKIKVSDSDLP
                     VNTLSGGNAQRVSIAKWLAIDPKIIILDAPTIGVDIANKEGIFQIIKSLAQQGIAVIF
                     VTDEVEETYYNSHRVIVMKKGRIVDEILPAYSSEKSIAEVIYENQQ"
     gene            4617..5576
                     /locus_tag="PXD71_001718"
     CDS             4617..5576
                     /locus_tag="PXD71_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6291818.1"
                     /translation="MKISNNHILSSTLLILILLLTLFTRDFATIENLYDVVNNYAMLM
                     ILACGLFVVLLSGGIDISFPAITIISQYVMVTLIGKYDVGFAGVFVISSTIGILLGFI
                     NALFVNRLSVPSIIITISTLNIFYGGLLYFTKGVWLYEYPEWFTQEIVFFKQITAEGV
                     EFGLSFQALMAILAVILTGLITNKLTIGRKIYALGGNPDATSRIGFNLLFLHLFAYGY
                     MGFMAGLAGIVQSYTVQSVAPDSLLGYELTVLAAVVLGGASLSGGRGTLLGTVMGVML
                     LAIIQNGLNLMNVSSYWQTIITGIIIILSISATALSQRKKQGA"
     gene            5579..6529
                     /locus_tag="PXD71_001719"
     CDS             5579..6529
                     /locus_tag="PXD71_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6291819.1"
                     /translation="MKSKLDPTIFYLLALFAVAVIAFSIFIPDIFWSVSNFQSMASQI
                     PVLGILTLAMALPMLTGGINLSIIAGMNACSLIIAYFITQYTGISWLFIAILLSLCCA
                     LAIGLLNGTLIAIIRVSPILATLGTMTLINGINILVTNGSTIANFPETMLSINASAFL
                     WIPLPILIFLFLAGLIWFFLEKTAMGKAVYFIGNNEKATYYSGINTKKVLIWVYVISS
                     ILCVFAAILMMSKLNSAKASYGDSYLLISILAAVLGGINPDGGKGKLLGIMLALVLLQ
                     VIESGLNMLGISSYITMILWGSLLLFFIFLQKNRLFRLTQ"
     gene            6542..8137
                     /locus_tag="PXD71_001720"
     CDS             6542..8137
                     /locus_tag="PXD71_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL6291820.1"
                     /translation="MQNEKQLIEQGNTVIGIELGSTRIKAVLISSDGTILATGGADWE
                     NRLIDGIWTYHQNEIWEKLQAAYMDLSKTVKEKYHTTIRTAKALGISAMMHGYLPFDK
                     HGNPLVAFRTWRNNITLTSSEKLTALFQYPIPQRWSIAHLHQALLNQEPHLPEIDYLT
                     TLSGYVHWQLTDHKVLGIGDASGMFPIDTELKSYNPTMLAQFDRAIADYRMPWKLEAI
                     LPQVRVAGEQAGKLTEKGAKLLDPTDNLQAGIPLCPPEGDAGTGMIATNCIKEKTGNI
                     SAGTSAFAMIVLEKALSKAYSELDIVTTPAGKLVAMAHANNCTSDINAWVNLFGECLS
                     VFGVNICTEELYEMLFLHALQGDADCGGLLSYGFHSGEHNVGLSEGCPVFLHPTNAHF
                     NLANFMRTHLYTAFGAMKLGMDILINQEKVEISRILGHGGIFKTEGVAQKILSSALNI
                     PLATASTASNGGAWGIALLADFLSVSDRYSLEEYLDNCIFNTTKLNLVQPDKTMSEGY
                     ERFMQRYKQGIPVVEKALFLNTD"
BASE COUNT         2419 a         1556 c         1607 g         2602 t
ORIGIN      
        1 cttttacagc tgagccattt ttgctttttg aatatgcctt cctcttatca attagtctta
       61 attaacctac tttatatctt cggtattggt ctaaatattg tactgcttag gtttctcagt
      121 ttgcattttg acccattaaa taataacggt atacgttttc ttgttggtgg gttcacctta
      181 ttatttttca ttttatggag attccgcaca tcgctgtctc agctgattaa aactccaaaa
      241 ttgattgcga tttctctatt tgtcgggtta atgatgtccg caaatatgta tttttggcta
      301 aaaggggtca aaattacaaa tgctgttacc gtctctattt ttggggtatt agctatgcca
      361 tttggtgtga ttattgccgc catattcttt caagatgaaa gagaacgcat taaaagcaaa
      421 gctttctggt tcggctcaat tttaacgatc atcggttcac tcggatttat ttggcacggg
      481 aaaacactct cgattggcga aggatttatt cttggttctg tgttcttatt tttgtcgatc
      541 attatccgta atatacaaaa cctagttgta aaatttgctc gagaactgaa cgtattaacc
      601 ctaagttgtg taacatcttt aactacttca ctctcgagtc tattacttag ccaacaagca
      661 ggtaaattta atgagctttt tactgcacca aactggctac tattctctct gatggttgct
      721 ggtatttatg cgattatgat cggaatggta ttaacctttc acattatcca aacccaagga
      781 ctgataacct accaagtatt ggaactatta atgccggttt caacagccat cattgcttat
      841 attttactgg acgaacgtat ttccattgtt caaatgggct ttgcttgtat tgtgatttta
      901 ggctcaagta tcgccttaaa gttagtaaaa tatcctaagc actattagga tctttgctaa
      961 ttaatattgg taaaattaca tttggtttac taatctgagc gttcattcat tagtaatgat
     1021 aaacaagcgg ttattttttg ttattttttg gtaaatactg atagtcatgt ataaaaacct
     1081 ttctttgctt tcaccgactt atccatttga ccttgaattc atcgccggtc ttactcaatg
     1141 tgagaaaggt aactatcttg attctattgt cgataggcaa caaggaatgc acggttatat
     1201 gcttcaatta accacttttg gtcgtggttc tgtttttgat ggtaagcacg tttttaccgc
     1261 tcatcgaggg cagttactcc tctttacacc caatgccgtt caacactacc accgtcaccc
     1321 ggaaagccaa tattggcact acaaatgggt ctattttcag ccgaatccta aatggaataa
     1381 atggctgaat tggtcagata tggaagaaaa aataggacgt atcaccatta gcgatgatcg
     1441 ttactttcag gaaattagcc aacttttcag taaaattgaa ttagaactta aatccagtaa
     1501 attttttaaa gaagatattg caacaagttt gttggaatac ttacttatga aatgtatttc
     1561 cgccgagaaa attgaggtta ttcctgtcat tgatcaacgc attttgacag tttgtgattt
     1621 aatcttaacg aatttggcta aaaatgagag cttagaagca atggcagaaa aagtattttt
     1681 atcgccatct cgcctctcac atcttttcaa gcaatcgctg gggatcagct tagtgcaatg
     1741 gcgggaatta caacgcattt ccgaatccaa aaaattgctt tatttctcta acatctctat
     1801 ctctaatatt gcgaaatcct taggctatga ggattctctc tacttttcca aaattttcaa
     1861 aaagcatacc gggctgtcac cctctgagtt tagaacctta gaacggcgta aagacagtga
     1921 aatgtgatct tgatctcact ttactttgca tagcagaatt ttccatatcg ataacaaaaa
     1981 gttacctgtt tttctctcat tcaaaagcat aagatacaaa cagctttaat agtgctgttt
     2041 ctattagaga agggatatca agctctgtga gtgatattca gtgaaataac ttcttatgta
     2101 tgagggaaga acaatgaaaa aaccaacgtt caagcacgca ttattttcaa gtattttagc
     2161 tttagcctgt tacggctcgg catttgccaa tgatgttgtt aatatctcca aaattgacgg
     2221 tatgccgtgg tttaatcgta tggcagaggg tgttgtgcaa gcaggcaaag ataacaatat
     2281 caatgcttat caagttggtc catcaaatac cgatgcacca caacaagtaa agttaattga
     2341 agatttaatt gcgaaaaaag ttagtgctat ttccatcgtc cctaacgatg caaatgcctt
     2401 agaaccggta ctgaaaaaag cgaaacagaa cggtattgtg gtattaacca atgagtcagt
     2461 cggacaacct agtgccgatt gggatattga aattatcgat aatgcacaat ttgcagcaga
     2521 ctatgtagaa gaagtagcaa aagctatggg cggaaaaggc ggctatgtga tttatgttgg
     2581 tggtttaacc gtgcctcaac ataatctgtg ggctgattta ttcgtgaaat accagaaaga
     2641 gcattatccg gatatgtttg aagtgaccag cagaatgcct gttgccgaaa atattaatga
     2701 tgcacgccgt accaccattg atttagtgaa agcccattcc gatcttaaag cggtcgtttc
     2761 ttttggttca caaggtccta ttggtgcagg gttggcagtg aaagaaaaac gcttaaaaaa
     2821 taaattaaat gtcttcggaa tgatgattcc ttcccaagca gccaatttaa ttaaaagcgg
     2881 tgatattaca atgggaatta cctatgaccc ggccaatgca ggttatgctt tagctgctgt
     2941 cgctgccaaa gtattaaaag gcgaaaaaat tgaggatgga ttagagatcc caacggtcgg
     3001 taaagccaaa gtagatagtg agaaaaaact acttcaattc cacaatgtgc tgaaagtcac
     3061 caaggaaaat atcgataaat tatattaatt tgcttgaaat atgggctgat agccagccca
     3121 tattttccct caggaggcac ttatggcatt tatttcgatg cggcatatca gtaaaacatt
     3181 ccacggcata aaagcattaa atcaagtcaa tctctcatta gattatggcg aagcactttg
     3241 tcttgccggt caaaatggct gtggaaaatc aaccttgatc aaaattcttt ccggcgttta
     3301 tcaaccggat aaaggggctg aaattcaaat tggtgcaacc caatatacca agctgacacc
     3361 acaggaatcg attgataaag gtattcaagt gatttatcaa gatttagccc tcttccccaa
     3421 tttaaccgtg gcagaaaata ttgccattaa cctgcaccga aaatttggct gggtgagtca
     3481 agatcacatt catcaggttg ccctcactgc tattcaaagc attaatgcaa atctagacct
     3541 gaatgcaata ttagaagatt taccgatcgc tcaacaacag ttagtcgcta tttgtcgagc
     3601 gttagcacaa aatgccagat tattgattat ggacgagccg actgcctcac ttactgcaaa
     3661 agaggtcaaa gatctgttag atgtcgtcat caaacttaaa agcaaaggca ttagcatcat
     3721 ctttgtcagt cataaattgc aagaagtaat gagtgtttcc gatagcgtat tagtgctgaa
     3781 agatggcaat atggttgggc aatatcctat ccgtgaaatg aatgaaaaac gccttggttt
     3841 cctaatgact gggctggaaa tcgattacaa gcggttagat ttgccggatt ttttgcaaag
     3901 aaaaaccgta ttggaagtcc gcaatttaac gctgcctcat cagtatcaag agattaactt
     3961 ttcgctacga gaaggggaaa ttgttgccct tacaggattg ctcggttcag ggcgtactga
     4021 gctttgccta agtttattcg gcattactca accacaatcc ggcgatattt tattaaacgg
     4081 tgaaaaagtg atatttaaca ctaaccgtga tgccattgcc aaagggattg cttatgtatc
     4141 ggaagataga atgcacaccg ggctgattat ggcagagtcg attcatcaca atattatttc
     4201 gactattttt cacaaaatca ccgataaatt acagattatt caatcctcga aggcttatgc
     4261 aagtagccaa gccttgattg attcattaaa gattaaagta tccgattcag atttgcctgt
     4321 taataccctt tccggaggga acgcacagcg agtttcgatt gcaaaatggc tggcaatcga
     4381 ccccaaaatt attattttag atgccccgac tattggggtc gatatcgcca ataaagaagg
     4441 gatcttccaa attattaaat cattggctca gcaaggcatt gcagttattt ttgtgactga
     4501 tgaagtggaa gagacctact acaacagcca tcgggtaatt gtaatgaaaa aaggtcgcat
     4561 tgttgatgaa atcttgccgg catattccag tgaaaaatcg attgcggagg ttatttatga
     4621 aaatcagcaa taatcatatt ctttccagta cgttactgat tttaatccta ttattaaccc
     4681 tatttactcg tgattttgca actattgaga acctttatga tgttgtaaat aattacgcaa
     4741 tgttaatgat tttagcttgt ggtttatttg tggtattgct gtctggcggc attgatattt
     4801 ctttccctgc tatcactatt atttcccaat atgttatggt cactctgatc ggtaaatatg
     4861 atgtaggatt tgccggcgtt tttgttattt cttccaccat cggcatatta ttaggtttta
     4921 ttaatgctct atttgttaat cggcttagtg tcccttccat tattattacg atctcaacac
     4981 tcaatatttt ttatggcgga ctactctatt ttaccaaagg tgtatggctt tatgagtatc
     5041 ccgaatggtt tacccaagaa atcgtgttct ttaaacagat aactgctgag ggagtggaat
     5101 ttggattatc gtttcaagca ttaatggcta ttttggcggt gatattaacc ggcttaatta
     5161 ccaataaact cactattggt cgaaaaattt acgctcttgg tggtaatccg gatgcaacct
     5221 cccgtattgg gtttaatctc ttatttctcc atctatttgc atacggttat atgggcttta
     5281 tggcaggctt ggcaggcata gtgcaatctt ataccgtaca atctgtcgca ccggattcac
     5341 tactgggtta tgaattaacc gtcctagctg ccgttgtatt aggtggtgct agtttatcag
     5401 gtggtagagg caccctgctt ggtacagtaa tgggcgtaat gttactggcg attattcaaa
     5461 acgggctaaa tttaatgaat gtatcgtctt actggcaaac cattattacc ggtatcatca
     5521 ttatcttaag tattagtgca acagccttaa gtcagcggaa aaaacaagga gcataacgat
     5581 gaaaagtaaa ctggatccaa cgattttcta tctcttagca ctcttcgcag ttgcagttat
     5641 cgcctttagt attttcattc cggatatttt ttggtctgtc agtaactttc aatctatggc
     5701 atcacaaatt ccagtattag gtattttaac cctagcaatg gcattaccga tgttaacagg
     5761 tggaataaat ctttccatta ttgccggaat gaatgcttgc tcattaatta ttgcctactt
     5821 tattacgcaa tataccggta tttcttggtt atttattgct attttattaa gcctttgctg
     5881 tgccttagcc attggattac tcaacggcac attaattgcc attattcgtg tttcaccaat
     5941 tttggcaacc ttaggcacaa tgactttaat taacggcatt aatattctag tcactaacgg
     6001 ttcaaccatt gcgaactttc cggaaacaat gttgagtatt aatgcttcag cctttttatg
     6061 gattccgcta cccattctaa tctttctctt tcttgctggg ctgatttggt ttttcttaga
     6121 aaaaactgca atgggtaaag cggtttattt catcggcaat aatgaaaaag ccacctatta
     6181 ttcgggtatt aataccaaaa aagtgctgat ttgggtttat gttatctcct caattctttg
     6241 tgtatttgca gctattttga tgatgtctaa actcaattct gcaaaagcct cttatggtga
     6301 ttcctatttg ttgatctcca ttttagccgc agtattgggc ggtattaacc ctgatggagg
     6361 taaagggaaa ctattaggga ttatgctcgc cttagtttta ttacaagtga ttgaaagtgg
     6421 cttaaatatg ctcggcatta gcagttacat tacaatgatt ttatggggaa gcttattgtt
     6481 attctttatc ttcctccaaa aaaatcgcct tttccgacta acacagtaat ggtggtagat
     6541 tatgcaaaat gaaaaacaac tcattgaaca aggtaatact gttattggta ttgaattggg
     6601 ctcaacccga attaaagcgg tgttaattag ctcagatggg acgattttag caaccggtgg
     6661 ggcggattgg gaaaatcgac tgattgacgg tatttggacc tatcatcaaa atgaaatatg
     6721 ggaaaaacta caagctgctt atatggattt aagcaagaca gtcaaagaaa aatatcacac
     6781 aaccattcgc acagccaaag cattgggaat tagtgcgatg atgcacggct acttaccttt
     6841 cgataaacac ggaaatccgc tggttgcctt tagaacttgg cgaaacaaca ttaccctaac
     6901 ctcatcggaa aaattgaccg cactctttca atacccaatt ccgcaacgtt ggagtattgc
     6961 tcatttacac caagcattgc ttaaccaaga gcctcacttg ccggaaattg attacctgac
     7021 aaccctctca ggttatgtgc attggcaact gaccgatcac aaagtgttag ggattggcga
     7081 tgcttccggc atgttcccga tagatactga gttgaaatcc tacaatccaa caatgctggc
     7141 tcaatttgat cgagccattg ccgactaccg tatgccttgg aaattggaag cgattttgcc
     7201 acaagttcgt gttgctggtg aacaagcggg caaattaacc gaaaaaggtg caaaattgtt
     7261 agatccaacc gataatttgc aagctggcat tccactttgt ccacctgagg gtgatgccgg
     7321 aacaggtatg attgcaacca actgtatcaa agaaaaaacc ggtaatattt ctgccggcac
     7381 ctccgcattt gcaatgattg tgttagaaaa agccctatcc aaagcctatt cagagctgga
     7441 tattgtgacg accccggcag gtaaattagt ggcaatggct cacgctaata attgcacctc
     7501 tgatattaat gcgtgggtga atttattcgg cgaatgctta tcggtttttg gcgtaaatat
     7561 ctgcaccgaa gaactttatg aaatgttatt tctccatgcc ttgcaaggcg atgcagattg
     7621 tggagggcta ttatcctacg gcttccactc cggtgaacat aatgtcggtt tatccgaagg
     7681 ctgtccggtt ttcttgcatc caacgaatgc ccattttaac ttagcaaact ttatgcgaac
     7741 ccatctctat accgcttttg gtgcaatgaa attaggaatg gacattctca ttaaccaaga
     7801 aaaagtggaa atttcccgta ttctcggtca cggtggtata tttaaaacag aaggtgttgc
     7861 acaaaaaatt ctgtcttccg cactgaatat tccattggct accgcaagta ctgcttcaaa
     7921 tggcggagct tggggaattg cattactggc agactttctc agcgtatcag accgttacag
     7981 tttagaagaa tatttagata attgcatttt caacaccacc aaactcaatc tggttcaacc
     8041 ggataaaaca atgagtgaag gttatgaacg ctttatgcaa cgttataagc aaggcattcc
     8101 tgtggtagaa aaagcgttgt ttttaaatac agattaaata attataattt aagtatcaca
     8161 agcggttatt tttgcaaaaa acga
//
LOCUS       DANLXS010000102         8145 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.102, whole genome shotgun sequence.
ACCESSION   DANLXS010000102 DANLXS010000000
VERSION     DANLXS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.102"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1995
                     /locus_tag="PXD71_001721"
     CDS             <1..1995
                     /locus_tag="PXD71_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6291821.1"
                     /translation="EIEQTRAAYDRLKRSGVASQNELRRASEATKQRVRELNAELGKT
                     SFGDKAGSFGRGLMGAGVGIAAGAAMARQPARNQMEFDRQLAMVANTAFSDRDVKGRI
                     DGKRELLDAVQKAVSVGGGSKEEALSSLDALLASGAVSTETALNLLPTLQKGAVATGA
                     SANDMAQIAISSMQQFGIKEEDIGRVLDMAVAAGQAGSFELADMASWLPQQMAAAKQA
                     GLSGIEGFERLLIANQQARVTAGTSNEAGNNLVNLLGKITAKETNERFKNIEYKDPKT
                     GKTKAIDFAKSMEHYKGKGQDSLQAFMSIMDDVVGSDKRYQELQAKLKTAKGEEQKRL
                     FNELTNLVEGTAIGEIISDRQALMALLGIKNNVQLGEKVQKDIQNSEGAVETSHAVIR
                     DTNAHKVESLKNTTEFAQMKNFEKVNGVLGTLAELLNKYANEYPNLTQFLSGATDAVK
                     IFGAALAASSVLDLLTGKKDKGGLIGDVLDKSSKASGGKGKVGKLLSVGGSFASVAGV
                     ATMLHGDQAPMSEERKAELRDPNYQARKMYEYKKEILGSRFHSFFRSNETLEQYRKDV
                     SAYEAKQKNSNTMGAVQGVLSTIPSLQELRPKVDSVTQTLSTYQADFQAFGATISAGI
                     EAGLASQSHTIANQITVELDGSVVAEKVAEYQFNFNKRMA"
     gene            1995..3287
                     /locus_tag="PXD71_001722"
     CDS             1995..3287
                     /locus_tag="PXD71_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HDL6291822.1"
                     /translation="MSGWTMPIQQASFKGVRFDVLAVDDSFERAVVEHAYPFVNGADL
                     EDMGLNTQTIRLQAVFFGSSYYTDYIRLLSVLQQKGADVLVHPIRGRLPNMLLVSANL
                     HTDAENINYVALDLTFKEATEMQPIFAFEDSLISKIDKLLLDLENLFDDGLRFWDSVG
                     VFDLKSRLLGLWGALFASFEAVRNLFDLDKKKYSIASSTSQRTYLRDGSGALNQLKEM
                     VDSGIQGRADLTTLSFKSQLLNITDAIDNTQAIVSKVARDEGFNRSKTVKLLGSDVVE
                     LGIMLDLIATTKLTKVLTELVEEREEELLIGDLELINQIVREHWLNIINKLREQQKNT
                     QKGVPSDKTKVIYETAEKLINNIRNVAHQFTALVLAVINKKPPLTVRMVMDSGTIHKV
                     AHAFYGDYERADELLRLNPHIRQPNFIERGTLLNCYWE"
     gene            3290..4417
                     /locus_tag="PXD71_001723"
     CDS             3290..4417
                     /locus_tag="PXD71_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6291823.1"
                     /translation="MENEIVVEIDGSQHKNWKSYDIDSDFLIPADSFNFELGKSSNIE
                     VLPNYSGKTAIVKINGKTVLTGIVDTTHHTISKSGRGFSLNGRDKAAILVDCSAPITN
                     VQGLSLLDAVKKIVTPLGIKEVQLKAEKNPTLSKVDIDVGESAWEAIMRCANSAGLHC
                     WFDPKGVLIVGGADYAQPPVATLYCVKESGDSRKNNFVDATLSYDVSQSYSEVTFLAQ
                     KHGKDSDNAKHDFKWVYKNEEMELYKPKTIVLGDVENLEALMKQAKKQVSDWQLESFN
                     LTIIVPDHKTKDGTLWQAGQRVHVVCEEYDLDGIFFLMGRRFRLSRTGGTTTELRLKL
                     DGVWTPDAYQAKAQEARKRKGKKGRKKKEPVEYVGSWELGK"
     gene            4419..5081
                     /locus_tag="PXD71_001724"
     CDS             4419..5081
                     /locus_tag="PXD71_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL6291824.1"
                     /translation="MMRKLAKQTKEVAKGVQDSVRAAFRGVLNLVKSGSDIQQVQVSA
                     LADETIQDLELMQHFGFTSVPPAGTQAVVIPLGGKTTHGIIVATENGSFRVKNLKNGE
                     TAIYDSSGSTIILKNGRVIDIECDSFNIKCKQYSVTASGGANFNTPKLETDQQFVSQG
                     QISGNGGMVVQGGSGASFTGSLTQQGGSISTDGDVVASGKSLVTHQHNEQGDGRPTSA
                     PI"
     gene            5200..5550
                     /locus_tag="PXD71_001725"
     CDS             5200..5550
                     /locus_tag="PXD71_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDL6291825.1"
                     /translation="MDREISPLTRDYTSENITTLQNAVYIRLTTPKGSWWADGRVGSL
                     LHLIQREKDLSRVGLLAQQYAEEALQPIIDDGRAKSITVTHEQPKDGSLNLLISVIDN
                     RGVKFEFKHPVKLV"
     gene            5563..6624
                     /locus_tag="PXD71_001726"
     CDS             5563..6624
                     /locus_tag="PXD71_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL6291826.1"
                     /translation="MYQIPTLEDVRREILRDIQSLEPTADIEVDSDYFVRASALASCV
                     VGLYAHQNWIVRQYFPDTADSEYLEMHAKLRNLYRKNATYASGSLKVFGTAGSVINEG
                     LQVKFGERFYVTKSSDVIGDDGSATVRVISMATGASSNVKSESKAQLTAAPAGVSSDC
                     LLLPDVTGGTEVESDAALLARLLEQIRRPPSGGNKNDFRQWAESVDGVTSAFVYPLRR
                     GEGTVDIAITSGDTVPSDEIVRATQAYIDEVRPVTAKSVYVLKPVEKRVNFNIKVQLD
                     TDTTLDGVKAEIENALENYFLSLKPADTLIISQIEAVISDLVGVVDREIVQPTRNQTI
                     DAYREMGWFRLGDVTVELM"
     gene            6624..7214
                     /locus_tag="PXD71_001727"
     CDS             6624..7214
                     /locus_tag="PXD71_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDL6291827.1"
                     /translation="MVTINSKHGKALGGLLPPVSYDPNGRWLALSLEVEGRELDRIDA
                     RAKTLIDAVDVSTGIFIDDWERVCGLTANSSLPLETRIERVIAKLNQLGGLSIGYITQ
                     VAKELGYCIKISEPQPFIAGLSRAGDMLWHKDIMWTFFVDICNPTDDYKRFRASVSMA
                     GDALMGRYKDPILESLLEEVKPAFSRVWVRYLGDIE"
     gene            7211..>8145
                     /locus_tag="PXD71_001728"
     CDS             7211..>8145
                     /locus_tag="PXD71_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6291828.1"
                     /translation="MKQVMNPINTPTQRFKDGNPATGEYGTIVTAEFLNNVQDSVINT
                     QQELHSVLAEAGIEANDEQVNQVAKAIKKIAGDATRDNFNALANPDGYKHIGRCKSVA
                     ELRTIRPTEHGQRILVDAYYEDGTTGGGEFVADLQDLVTPDDGGVCFVVNNNGGRWKR
                     VDLSHLTLFDFWAVGDGVTNDESAFVNAMRYSQFFIENGTFRINNAVNSVRDNVKILG
                     NKTGKLVLGAGIQQAGAEVFNINHSNYFISGFCIETPNKAIGIRFKSLDDAGVKNLHI
                     DNVVFNGTFYGVRAGESIQADTNYPTDNVIVQNCQS"
BASE COUNT         2387 a         1493 c         2061 g         2204 t
ORIGIN      
        1 gaaattgaac aaactcgtgc agcctatgac cgcttaaaac gctcaggtgt tgcctcgcaa
       61 aatgagctac gccgtgcctc agaagcgact aagcaacgag tccgagagtt aaatgctgaa
      121 ttgggtaaaa ccagttttgg ggataaggca ggaagttttg ggcgtgggtt gatgggtgca
      181 ggggttggaa ttgctgcagg tgcagcaatg gctcgccagc ctgcaagaaa tcaaatggaa
      241 tttgatagac agcttgcaat ggtagcgaat accgcctttt cagatcgtga tgtgaaaggg
      301 cgtattgatg ggaaaagaga gttacttgat gccgtacaga aagcagtaag tgtaggcggt
      361 ggctcaaaag aagaagcgtt gagctcacta gacgctttac ttgcctcagg tgctgtttct
      421 accgaaaccg ctctcaattt actcccaacg ttgcagaaag gtgcggtcgc aacaggggca
      481 agtgcaaatg atatggctca aattgctatt tcctcaatgc aacaatttgg gattaaagaa
      541 gaggatattg gtcgagtctt agatatggca gttgccgctg ggcaagccgg tagctttgag
      601 cttgcagata tggcaagttg gttgcctcaa caaatggccg ctgcaaaaca agctggctta
      661 agcggcattg agggatttga gcgactttta attgcaaacc aacaggctcg tgtcactgcc
      721 ggtacaagta acgaggctgg taataattta gtgaatctgt tgggtaagat tactgctaaa
      781 gaaactaatg agcgttttaa gaatattgaa tataaagatc cgaaaactgg caaaaccaaa
      841 gcgattgatt ttgcaaaatc tatggagcat tacaagggaa aagggcaaga ttcattgcag
      901 gcttttatgt ctattatgga tgacgttgtc gggagtgata aacgctatca agaactacaa
      961 gctaaactta aaactgctaa aggcgaagag cagaaacgct tattcaatga attaacaaac
     1021 ttagttgaag gtacggcaat tggagaaatt atttctgatc gccaagcatt aatggctcta
     1081 ttagggataa agaataatgt tcaattaggt gaaaaagtac agaaagatat tcagaatagt
     1141 gaaggagctg ttgaaacctc tcacgctgtt attcgggata ctaatgctca taaagttgaa
     1201 tcattaaaaa atacaactga atttgcacaa atgaaaaact ttgaaaaagt aaatggagtg
     1261 ttaggcacat tagctgaatt actgaataaa tacgccaatg aataccctaa cttaactcaa
     1321 tttttaagtg gtgcaacaga tgccgttaaa atttttggag cagctcttgc tgcatcaagt
     1381 gtgcttgatt tattaaccgg taaaaaagac aagggcggac taattggtga tgttttggat
     1441 aaatcatcaa aagctagtgg aggtaaaggt aaggtaggta aattgctttc ggttggtggc
     1501 tcttttgcta gtgttgcagg cgttgccaca atgctacacg gcgaccaagc cccgatgagt
     1561 gaggagcgaa aagcagagtt aagagatcct aattatcaag ctcgtaaaat gtacgagtac
     1621 aaaaaagaga ttttaggtag ccgtttccac tcgttttttc gtagtaatga aacccttgag
     1681 caatatcgga aagatgtgag tgcttatgag gctaagcaaa agaatagcaa tactatggga
     1741 gctgtacaag gggttttaag taccattccg agcttacaag agttacgccc taaagttgat
     1801 tcggttactc aaaccctatc cacctatcaa gccgactttc aggcttttgg ggcaacgatt
     1861 tcagccggta ttgaggcagg gcttgcctcg caatctcata cgattgcaaa tcaaatcacc
     1921 gtggagctgg acggttcggt ggtggctgaa aaagtggctg aatatcagtt taattttaac
     1981 aagaggatgg cgtaatgagc ggttggacaa tgcctattca acaggcgagt tttaaaggtg
     2041 tgcgttttga cgtgcttgca gtggatgata gctttgagcg agcggtagtt gaacacgcct
     2101 acccttttgt aaatggggct gatttagagg atatggggct aaacacccaa actattcggc
     2161 tgcaagcggt cttttttggc tcaagctatt acacggacta tatccgctta ctttcagttt
     2221 tgcagcaaaa aggggctgat gtattagtcc accctattcg tgggcgttta ccgaatatgt
     2281 tactcgtttc ggcgaatcta cataccgatg ccgaaaatat caattatgtt gccttagatt
     2341 tgacctttaa agaggcaacc gaaatgcagc cgatttttgc ctttgaagat tcgctgattt
     2401 cgaaaatcga caagctgcta cttgatctcg aaaacctgtt tgatgatggc ttgcgttttt
     2461 gggatagcgt tggggtgttt gatctgaaat ctcgtttgct tgggctttgg ggggcgttgt
     2521 ttgcctcgtt tgaggcggtg cgtaacctgt ttgacttgga caagaaaaaa tacagcattg
     2581 cctcatcaac ctcacaacgc acctatttaa gagacggttc gggggcattg aatcagttaa
     2641 aagaaatggt ggatagcggt attcaggggc gtgctgattt aacgacactc tcgtttaaat
     2701 ctcagttact caatattact gatgcgatag ataacactca ggcgattgtg agcaaagtgg
     2761 ctagagatga gggttttaac cgctcaaaaa cggttaagtt acttggctct gatgtggttg
     2821 agcttggcat tatgcttgac cttattgcca cgactaagct caccaaagtc ttgactgagc
     2881 tggtagaaga gcgtgaggaa gagctgctaa ttggtgattt agagttaatt aaccaaattg
     2941 tgcgtgagca ttggctgaat attatcaaca aactgcgtga gcaacagaag aacactcaaa
     3001 aaggcgtgcc gagtgataag acaaaagtta tctacgagac cgcagaaaag ctgatcaaca
     3061 atatccgtaa tgttgcccat cagtttactg cacttgtgct tgcggtaatt aataaaaaac
     3121 cgcctttgac cgtgcgaatg gtaatggata gcggcacaat ccataaagta gctcacgcct
     3181 tttatgggga ttatgagcga gcggatgagc tattacgtct taatccgcat attcgccagc
     3241 caaactttat tgagcgaggc acgctgttaa attgttattg ggagtaaaaa tggaaaatga
     3301 aattgtggtg gagattgacg gctcgcagca taaaaactgg aaaagttatg atattgacag
     3361 cgatttttta attcctgctg atagttttaa ttttgagttg ggtaaatcta gcaatataga
     3421 ggttttacct aattattcgg gcaaaacggc aatagttaaa atcaatggca aaacggtgct
     3481 cactggcatt gtggatacca cacatcacac cattagcaaa agcgggcgtg ggtttagcct
     3541 aaatggacgt gataaagccg ctattttggt ggattgctct gccccgatta ccaatgtgca
     3601 agggctctct ttgcttgatg ctgttaaaaa aattgtaacc cctcttggga ttaaagaggt
     3661 gcaactgaaa gccgagaaaa acccgacttt aagcaaggtg gatattgatg tgggtgaatc
     3721 ggcgtgggag gcgattatgc ggtgtgccaa ctcggcaggt ttgcattgtt ggtttgaccc
     3781 caaaggcgtg ttaattgtcg gcggtgcgga ttacgctcaa ccgcccgttg ccacgttata
     3841 ttgcgtgaaa gagtcggggg atagccgtaa aaataacttt gttgatgcaa cactttctta
     3901 tgatgtgtcg caaagctatt cggaggtcac ttttcttgct caaaaacacg gcaaagattc
     3961 tgataatgcc aagcacgatt ttaaatgggt gtataaaaat gaggaaatgg agctttataa
     4021 accgaaaact atcgtgctgg gcgatgtgga gaacctcgaa gccttgatga aacaggcgaa
     4081 aaaacaggta tcagattggc agttggagtc atttaacctc acgattattg tacctgacca
     4141 taaaaccaaa gacggcacgt tgtggcaagc tgggcaacgt gtgcacgtgg tatgtgagga
     4201 gtatgattta gatggtattt tctttttaat ggggcgacgt tttcgactct ctcgcacggg
     4261 tggcacaacc accgaattgc ggttaaaact ggatggcgtg tggacacctg atgcttatca
     4321 agctaaagca caagaggctc gcaagcgtaa aggcaaaaaa ggacgtaaga aaaaagagcc
     4381 tgtagaatat gtaggctcgt gggagttggg aaaataatat gatgagaaaa ttagcaaaac
     4441 agactaaaga ggtggcaaaa ggtgtgcaag atagcgtgcg tgctgctttt agaggggtat
     4501 taaatttagt caagagtggc agtgatattc agcaggtaca ggtgtccgct ttagcggacg
     4561 aaactatcca agatttagag ctaatgcagc attttggttt cacttccgtg ccacctgccg
     4621 gcacgcaagc ggttgttatc cccttaggtg gtaaaaccac gcacggtatt attgtggcga
     4681 ctgaaaacgg ctcgtttagg gttaaaaatc taaaaaatgg tgagacggct atttatgatt
     4741 caagcggctc aactatcata ttaaaaaacg ggcgagtgat tgatattgag tgcgatagct
     4801 ttaacattaa atgcaagcaa tattccgtca ccgcaagcgg tggagcaaat tttaacaccc
     4861 cgaaactaga aaccgaccag cagtttgtgt ctcaagggca gatcagcggc aacggtggaa
     4921 tggtagttca aggcggaagt ggtgcaagtt tcactggctc attaacacaa caaggaggca
     4981 gtatctcaac tgatggtgat gtggtggcat cgggtaaatc actggttaca catcaacaca
     5041 acgaacaagg cgatggcaga cctacctctg ctccaattta acttacaagc taatttacaa
     5101 gcggtctaat tttgcaaatt ttttgtagaa aaagaccgct tgccatttgt ggaagtctct
     5161 ccccgttact cctgtcttta atgtctctac catagcaata tggacagaga aatcagcccg
     5221 ctcactcggg actatacaag tgaaaatata actacactgc aaaatgccgt gtatatcaga
     5281 ctaactactc cgaaaggctc gtggtgggca gatgggcgtg taggttctct gctccatctt
     5341 attcaacgtg agaaagattt aagccgtgtg gggctattag ctcaacagta cgccgaagag
     5401 gctttgcagc cgattattga tgacggacga gccaaaagca ttacggtgac acacgagcag
     5461 ccaaaagacg gctcgttaaa tcttttaatc tccgttattg ataatcgtgg ggttaaattt
     5521 gagtttaaac accctgtaaa actggtttag gtggttttta aaatgtatca aatcccaacg
     5581 cttgaagatg ttagaagaga aattctgcga gatattcaat ctttagagcc aactgctgat
     5641 attgaggtgg attcggatta ttttgttcgt gcctccgctt tggcaagctg tgtggtgggg
     5701 ctgtatgctc accaaaactg gattgttcgt cagtatttcc cagatacggc agactccgaa
     5761 tacctcgaaa tgcacgctaa attgcgaaat ctttaccgta aaaatgcgac ctatgcgagt
     5821 ggttcgttga aagtttttgg tactgctggc tcagtaatca atgagggctt gcaagttaaa
     5881 tttggcgagc gtttttatgt aaccaaaagc tcggacgtta ttggtgatga tggctctgca
     5941 acggtgcgag tgatttcaat ggcaacaggg gcatcaagca atgttaaatc tgaaagtaaa
     6001 gcacaactga ctgctgctcc tgctggcgta tcaagcgatt gcttgttatt gcctgatgtg
     6061 acaggcggga cggaagtgga aagcgatgcg gcattgctgg ctcggttatt agagcagatt
     6121 cgccgaccac cgtcaggcgg gaataaaaat gacttccgcc aatgggcaga aagcgttgat
     6181 ggcgtaacct ctgcgtttgt ttatccatta cggcgtggcg agggtacggt agatattgca
     6241 atcacctcag gtgataccgt gccaagtgat gaaatcgtgc gagcaactca agcttatatt
     6301 gatgaagtcc gccctgttac ggctaaatcg gtttatgtac ttaagccagt tgaaaaacgg
     6361 gtcaatttta acatcaaagt gcaacttgat acggatacga cgcttgatgg tgtaaaagcg
     6421 gaaatcgaaa acgctttaga gaattatttt ttatcactca aacctgctga caccttaatt
     6481 atttcgcaaa ttgaggcagt aattagtgat ttagtgggcg tagtagatcg tgagattgta
     6541 cagccaacca gaaaccaaac gattgatgcc taccgtgaaa tgggctggtt cagattgggt
     6601 gatgtaactg tggagttgat gtgatggtaa cgattaactc taaacacggc aaggcattgg
     6661 gcggtttatt accgcctgta tcgtatgacc caaacggcag atggctagcg ttatcccttg
     6721 aagttgaggg gagagagcta gaccgcattg atgcgagagc caagacttta atcgatgccg
     6781 ttgatgtatc aacaggcatt tttattgatg attgggagcg ggtttgtggc ttaacggcga
     6841 atagctcact accgcttgaa acccgaattg agcgtgtgat tgcaaagctg aatcagttgg
     6901 gtggattgtc gataggctat atcactcaag tggccaaaga gcttggatat tgcatcaaaa
     6961 ttagtgagcc acaacccttt attgctgggc taagccgagc tggggatatg ttatggcata
     7021 aagacattat gtggacattt tttgtggata tttgcaaccc cacagatgat tacaagcgtt
     7081 ttagagcaag tgtgtcaatg gctggcgatg cgttaatggg acgatacaaa gacccaattt
     7141 tagaaagtct acttgaagag gtaaaacctg catttagccg tgtgtgggta agatatttag
     7201 gagatattga atgaaacaag tgatgaatcc gattaatacc ccaacacagc gttttaaaga
     7261 tggcaaccct gccactggtg aatatggcac gattgtgact gctgaatttt tgaataacgt
     7321 ccaagattcg gtcattaaca cccaacaaga attgcacagt gtacttgctg aagcaggaat
     7381 cgaagcaaat gacgaacaag ttaatcaggt ggctaaagca atcaagaaaa ttgcgggcga
     7441 tgctactcgt gataatttta acgcattagc caaccctgat ggttacaaac acatcggtcg
     7501 ctgcaaatcg gttgcggagt tacgtactat ccgcccaacg gaacacggtc agcgtatttt
     7561 ggtggatgct tactatgagg acggcacaac aggcggtggg gagtttgtgg ctgatttgca
     7621 ggatttagtt acgcctgatg atggtggggt ttgttttgtt gtgaataata atggggggcg
     7681 gtggaagcgt gttgaccttt cacatcttac attatttgat ttttgggccg tcggtgacgg
     7741 ggtaacaaat gatgaatcag catttgtaaa tgctatgaga tattctcaat tttttatcga
     7801 aaacggtaca tttcgtatca acaatgccgt taattctgtt cgcgataatg tgaaaatttt
     7861 gggcaataag accggcaaat tagtattagg tgcaggtatc cagcaagcag gggccgaagt
     7921 ttttaatatt aatcacagca attattttat ctcgggtttt tgtattgaaa caccgaacaa
     7981 agcaattggt attcgattta agtctttgga tgatgctggt gttaaaaatc tccatattga
     8041 caatgttgtg tttaatggca ccttctacgg ggtcagggca ggtgaaagta ttcaggcgga
     8101 tactaattac ccaactgata atgtgattgt acaaaactgc caatc
//
LOCUS       DANLXS010000103         8115 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.103, whole genome shotgun sequence.
ACCESSION   DANLXS010000103 DANLXS010000000
VERSION     DANLXS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.103"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(64..1812)
                     /gene="dnaG"
                     /locus_tag="PXD71_001729"
     CDS             complement(64..1812)
                     /gene="dnaG"
                     /locus_tag="PXD71_001729"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL6291829.1"
                     /translation="MKGTIPRSFIDDLIARTDIVEVINSRVKLKKAGRDYQACCPFHH
                     EKTPSFSVSQSKQFYHCFGCGVHGNVITFLMDYDKLEFPEAIEELAAMHGLEVPRENV
                     IQRDGKPQANFKTKRNLYELMEAVAKFYQQNLSHQIEPQSYLQQRGLSEEIIERFEIG
                     FAPNAFDAALRTFGQSQEDIQKLLDTGVLTKNDSGRIYDKFRNRVMLPIRDKRGRVIA
                     FGGRVLPNDEQGAKYMNSPETVIYHKGSELYGLYQALKVNETPDYLFVVEGYMDVVAL
                     AQFGVDNAVASLGTATTGEQIQQMFRCTEQIVCCYDGDRAGRDAAWRALENALPYLQD
                     GRQLKFIFLPDGEDPDTFVRSQGKEGFEQYVAQNAKPLSDFLFDSLLADVDLSSKEGK
                     SKLAALAVPLINRIPGEMLRVYLRNILGQKLGILDPTQIEALLPTKKAAEKPLHQAVK
                     MKRTPMRLLIALLIQNPYFVKFVPDVSALASLKDPGFTLLTDIVATCQQNAGISMGGL
                     LEHYREDPQYKIVEALANWDHLELPENIERTFIDTLDFLYAKLVELRKEELIAKDRTV
                     GLTDDEKKEYALLMFQ"
     gene            complement(1966..2181)
                     /gene="rpsU"
                     /locus_tag="PXD71_001730"
     CDS             complement(1966..2181)
                     /gene="rpsU"
                     /locus_tag="PXD71_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL6291830.1"
                     /translation="MPVIKVRENESFDVALRRFKRSCEKAGILAEVRAREFYEKPTTI
                     RKREKASLAKRHAKRNFRENARNTRLY"
     gene            complement(2329..2925)
                     /locus_tag="PXD71_001731"
     CDS             complement(2329..2925)
                     /locus_tag="PXD71_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HDL6291831.1"
                     /translation="MSLLPMNEITSLNQLDLQKSYSYADYLLWKFKERVEIIKGKIVA
                     MSPAPTRFHQRISMKMTAKFLEVFDNHQCQIYSAPFDVRFPDENGKVKTVVQPDLCVI
                     CDVSKLDERGCVGAPDLVVEILSPGNTKREMKDKYELYQEQGVKEYWIVSPETRTIQI
                     FVLENGKYIGIQPVAEDEPATSVVFPALSFSTEKLFEL"
     gene            3067..4323
                     /locus_tag="PXD71_001732"
     CDS             3067..4323
                     /locus_tag="PXD71_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL6291832.1"
                     /translation="MHLNFKKIDNSHIALFYNVLVATFFVTVLTFKKGYSYVPMALGI
                     IATLSFLFYRFKLKQKWLLDKEDKFFIFSLIGYFLTFAMSAIFNGDGIREIDNPIQIL
                     LLIPLIILFRLYPIKKETVLHFIPIGALVAGCIAIYQRFILNLKFPFPEIMRIQSGNI
                     AILLSLFSIAIAFYWLSKKRIKTGIAYLVFAMFGLFASILTGARGGWLAFIPICFFIL
                     FLNLRQLNKKAIFLILLALISSLSFFISKPEFGIQKRYNAAKNDIIVFLEKGNRHSSL
                     GARFDMWENALIGIREKPLLGHGSTGYETFKKKQIETKQMAKTTLRFNSLHNQYLEAF
                     VKRGVVGFMALMAILIIPFVIFAKRLKAKNREVKCMAILGIVHIISHSFFFLSQSFLA
                     HNSGSLFYFFVLILLYHLVKQNEEII"
     gene            complement(4425..5432)
                     /gene="rfbB"
                     /locus_tag="PXD71_001733"
     CDS             complement(4425..5432)
                     /gene="rfbB"
                     /locus_tag="PXD71_001733"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL6291833.1"
                     /translation="MIILITGGAGFIGSALVRYLIEHTPHTVINVDKLTYAGNLASLE
                     SVAHHERYHFEKADICASGQISEIFGKYQPDVVMHLAAESHVDRSISGAADFIQTNIV
                     GTYTLLEAARAYWDTLPEPRKSAFRFLHISTDEVYGDLAKSEELFTEKTPYNPSSPYS
                     ASKAASDHLARAWHRTYGLPTVITNCSNNYGAYQYPEKLIPLMILNALEGKSLPVYGN
                     GLQVRDWLFVEDHIRALYLVLQKGQIGETYNIGGHNEKTNIEVVRSICSILQEMVPNN
                     PLVVGQYADLITYVQDRPGHDFRYAIDTSKIEKELDWKPKETFESGLRKTVEWYLANR
                     N"
     gene            complement(5429..6010)
                     /locus_tag="PXD71_001734"
     CDS             complement(5429..6010)
                     /locus_tag="PXD71_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL6291834.1"
                     /translation="MLEKIEKLIDEINKLHLAFSKDYFETGKIEKVNLKHTFAKVPTP
                     HILAYRLNLHESINDYLYRADLEGISYFYRVKTSESILDKIERFKQRSEGYPVNSILN
                     DIFGARIIVSSEVIQQVMESLDYWQEKYGLKNWYLRDKDEYVGIHIYFKNSSNFYYPW
                     ELQIWDKKDAEKNIQSHIKYKRNFVNQISYSPL"
     gene            complement(6031..7302)
                     /locus_tag="PXD71_001735"
     CDS             complement(6031..7302)
                     /locus_tag="PXD71_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL6291835.1"
                     /translation="MSIQQKKRHSRWYERIFFSTAVQSILGVMFSSVLPAIYFWGEKF
                     NSIETRSNTIIATSLAFVMIAISLRVLMKYPGNKSASFVFSTVISWYAVLLIVLTIFR
                     FDYSNTLLGTSFILTILFCFIGYFLSRSWIIPKIALVPFGRAINLNRIQNVEWIQLSE
                     PILGEKRINSIAVDLHYPDLSAEWHKFLAKCTLNGIPVYNVRQLEESLTGRVKIRHMY
                     ENDLGSLLPSPIYSLVKRIIDIALIVATFPITLPIMTITAIAIRLESEGGAMFIQKRV
                     GQGGKEFNIYKFRSMCKDSEKDDAQFASSADMRVTRIGKFIRKTRIDELPQFFNVLKG
                     DMSLIGPRPEQTAFVDKFEEEIPFYNYRHIVKPGISGWAQVVQGYAADVDDTKVKVEH
                     DFYYIKHFSLWLDILIIFKTLKTMLTGFGAR"
     gene            complement(7306..8114)
                     /locus_tag="PXD71_001736"
                     /pseudo
     CDS             complement(7306..8114)
                     /locus_tag="PXD71_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         2522 a         1676 c         1386 g         2531 t
ORIGIN      
        1 acttctgcca aatctcccct aacccctctt tgctaaagag gggaattatt cgagcttaaa
       61 attctactga aacatcaaaa gagcatactc ttttttctca tcatcggtta agcccacggt
      121 tcggtctttg gcaattagct cttctttgcg taattccact aatttggcgt agagaaaatc
      181 gagagtgtca ataaatgttc gttcgatatt ttcgggtaac tctaaatgat cccaatttgc
      241 gagggcttca acaatcttat attggggatc ttcccgataa tgttcaagta gcccgcccat
      301 actgatacct gcattttgct gacaagttgc gacaatatcg gttaataatg taaagccagg
      361 atctttcaag gaggctaaag cacttacatc gggtacgaat ttcacaaaat acggattttg
      421 aatcaataac gcaatcaaca gccgcattgg cgtacgtttc attttgaccg cttgatgtaa
      481 aggcttctct gccgcttttt tcgtcggtaa cagagcttct atctgcgttg ggtctaaaat
      541 gcctaatttc tgtcctaaaa tattgcgtaa atacactcgc agcatctcgc ccggaatacg
      601 attaattaac ggcacagcta aagcagcgag cttggatttt ccctcttttg aagagagatc
      661 cacatccgcc agcaaactgt caaataagaa atcgcttaat ggcttggcat tctgtgccac
      721 gtactgctca aatccttctt taccttgaga acgaacaaag gtgtccgggt cttcgccatc
      781 gggcaagaaa ataaatttca gttggcgacc gtcttgcaaa taaggcaacg cattttctaa
      841 cgcccgccaa gctgcatctc gaccggctct atcgccatca taacaacaga caatttgctc
      901 ggtgcaacga aacatttgct gaatttgttc accggtagtt gccgtgccta aagaggcgac
      961 tgcattgtcc acgccgaatt gggcaagggc gaccacgtcc atatagcctt ccaccacgaa
     1021 cagataatcc ggcgtttcat tgactttcag tgcttgatac aagccatata actcactgcc
     1081 tttatgataa atcaccgttt cgggcgagtt catatatttc gccccttgct catcattcgg
     1141 caacacccgc ccaccaaagg caattacccg tccacgctta tctcgaatcg gtaacattac
     1201 ccgattgcga aatttatcgt aaattcgacc gctatcattt ttggttaaaa cgcccgtatc
     1261 gagcaatttc tgaatatctt cttgtgattg cccaaaagtg cgtagggctg catcaaaggc
     1321 attcggagca aaaccaattt caaagcgttc gataatctct tcagacaaac ctcgttgctg
     1381 taaatagctt tgtggctcaa tctggtgcga taaattctgc tggtagaact tcgccaccgc
     1441 ttccatcaat tcataaagat tacgcttagt tttaaaatta gcttgaggct tgccgtcacg
     1501 ttgaatcaca ttctctctcg gtacttccag cccgtgcata gctgccagtt cttcaatcgc
     1561 ttccggaaat tcgagtttgt cataatccat caaaaaggtg atgacattgc cgtgtacgcc
     1621 acagccgaaa caatggtaaa actgttttga ttggcttacc gaaaaggaag gggttttctc
     1681 gtgatgaaaa ggacagcagg cttgataatc tcgcccagcc tttttgagtt tgacccgact
     1741 gttgatgacc tcaacaatat cggtgcgagc gataagatca tcaataaatg aacgtggaat
     1801 tgtgcctttc attgatttcc tcctgctctt tgaatgatac aagcggagca attttgcaaa
     1861 ttttttgcaa aaaacaaccg cttgaaatag aaacgttaaa aacaccaaaa ccgtgagcct
     1921 aacggaatca cggtttcgat atagtttgct aaccagccta actaattagt ataaacgtgt
     1981 gttacgtgcg ttctcacgga aattacgttt agcgtgacgt ttagcaagtg atgctttttc
     2041 acgtttacga attgtagttg gtttttcgta gaattcacga gcacgaactt cagctaagat
     2101 acccgctttt tcgcaagagc gtttgaaacg acgtaatgct acgtcaaatg attcattttc
     2161 acgaacttta attactggca tatgccatca cctcagaaaa atatgattaa attaaaccgc
     2221 acacaatttt ggcggcatag aacaaaaaag gtagtgcatt ctaccttaag cactcacata
     2281 agtaaagtag tattgtggca taaaaccgcc gtattatgtt gcatttttct acagttcgaa
     2341 taatttctcg gtcgaaaaac ttaatgccgg aaagaccacc gaagttgccg gttcatcttc
     2401 tgccaccggc tgaataccga tatatttacc gttttccaac acaaaaatct gaatagtgcg
     2461 ggtttcggga gaaacaatcc aatactcttt caccccttgt tcctggtaaa gctcgtattt
     2521 atctttcatc tcccttttgg tattacccgg agaaaggatt tccaccacta aatccggtgc
     2581 ccctacacag cctctttcat ctaacttact gacatcacaa attacacaaa gatcaggctg
     2641 cactacagtt ttgacttttc cgttttcatc gggaaaccga acgtcaaaag gcgcagaata
     2701 aatctggcat tggtgattgt caaaaacctc taaaaatttt gctgtcatct tcatagaaat
     2761 tcgctgatga aaacgagtag gagccggaga catcgccaca attttccctt tgataatctc
     2821 aacccgctct ttaaatttcc atagcaaata atcagcgtaa ctgtaacttt tttgtaagtc
     2881 cagttgattt aagctggtaa tttcgttcat tggtaataag ctcataccgc ctcctttatc
     2941 agaaatataa agatattatg ccgaataatc cctcaaattc aacaaaactt tcgctaaaaa
     3001 gagatagata acctttatac ccttagtata aaatgacagc aattttcatt gttttaggat
     3061 atgtcgatgc accttaactt caaaaaaata gacaactcac atattgctct tttctataat
     3121 gtactagtcg ccacattttt tgtcactgtt ttaaccttta agaaaggtta tagttatgtg
     3181 ccaatggcat taggtattat cgctacactt agtttcttat tttatcgttt caaattaaaa
     3241 caaaagtggt tacttgataa agaggataaa ttttttattt tctcgcttat tggctatttt
     3301 ctaacgtttg caatgtctgc tatttttaat ggcgatggca ttcgtgaaat tgataatcct
     3361 atccagattt tattgcttat ccctttaatc atcctcttta gactttaccc gattaaaaaa
     3421 gaaactgtat tgcactttat accgattgga gcgttggtag ctggttgtat agcaatatat
     3481 cagaggttta tattaaatct taaatttcct ttccctgaaa taatgcgtat tcaatcgggg
     3541 aacatagcaa tattattatc actatttagt atagcaattg ccttttattg gctttcaaaa
     3601 aaaagaatta aaactggcat tgcctacctg gtttttgcca tgtttgggtt atttgccagt
     3661 attctaacag gagcaagagg cggctggctt gcattcattc ccatttgctt ttttatccta
     3721 tttttaaatt taagacagct aaataagaaa gctattttcc tgattctatt agcacttata
     3781 tcttcactct cattttttat ctccaaacca gaatttggca tacaaaaacg atataatgca
     3841 gctaagaatg atattatcgt atttttagaa aaaggaaata gacattcatc tctgggagct
     3901 cgttttgata tgtgggaaaa tgcgcttatc ggaatccgtg aaaaaccgct attaggacac
     3961 ggtagcacag gctatgaaac attcaaaaag aaacagattg aaacaaaaca aatggcaaaa
     4021 acaacgctta ggtttaatag cttacacaac caatacctag aagcctttgt gaaaagaggt
     4081 gtagttggtt ttatggcgct aatggctatt ttaattattc catttgttat ttttgccaaa
     4141 cgattaaagg caaaaaatag agaggtaaaa tgtatggcga tattaggcat tgtacatatt
     4201 atttcgcatt cattcttttt cctaagccaa tcatttttag cccataattc aggctcatta
     4261 ttctatttct ttgtgttaat tttactctac catttagtca aacagaacga agagataata
     4321 tgataaaaca atagaagaaa gttgggaata taaccgaggt ggtaacttca tcttctacca
     4381 caggataaat gtcgggatat ttcctctttt tctgcttctt actactaatt ccgattagcc
     4441 aaataccatt ccactgtttt acgcaagcca ctttcaaaag tttctttcgg tttccagtcg
     4501 agttcctttt cgatttttga agtatcaatt gcatagcgga aatcgtggcc agggcgatct
     4561 tgcacataag taattaaatc agcatattgt cctacaacaa gcgggttatt tggcaccatt
     4621 tcttgcaaaa tagaacagat agaacgaacc acttcgatat tggttttttc attatgcccg
     4681 ccaatattgt aggtttctcc gatttgccct ttttgcaaca ccaaatagag ggcacgaata
     4741 tgatcttcca caaacagcca atctctgacc tgcaagccgt tgccgtaaac aggtagggat
     4801 ttaccttcta aggcatttaa aatcattaac ggaataagtt tttcgggata ttggtatgcc
     4861 ccataattat tggaacagtt ggtgatgaca gtcggtaaac cgtaggtgcg atgccaagcc
     4921 cgagccagat gatcgctggc agctttagat gccgaatagg ggctacttgg attataaggg
     4981 gttttctcgg taaataattc ctcgcttttg gctaaatcac catagacctc gtcggtcgag
     5041 atatgtagaa agcgaaacgc cgatttcctt ggctcaggta acgtgtccca ataggctctc
     5101 gctgcctcta ataaagtata agtgccgaca atattggttt gaataaaatc tgctgcccca
     5161 gaaatagagc gatcaacatg gctttccgct gctaagtgca ttaccacatc aggctgatat
     5221 ttgccaaaaa tctcagaaat ttgaccgctt gcacaaatat ctgctttttc aaaatggtag
     5281 cgttcgtgat gtgcgacact ctccaatgag gctaaatttc ccgcataggt gagcttatcc
     5341 acattgataa ctgtgtgtgg agtatgttca atcagatagc gaactaatgc agatccgata
     5401 aaaccggctc cgccagtgat gaggatgatc ataaaggtga atatgaaatt tgattcacga
     5461 aatttcgttt gtatttaata tggctttgaa tattcttttc agcgtctttt ttatcccaaa
     5521 tttgcaattc ccaaggataa taaaagttac ttgaattctt aaaataaata tgaataccca
     5581 catattcatc tttatctctc aaataccaat tttttagtcc atatttttct tgccaataat
     5641 ctaatgactc cattacttgc tgaatcactt cactagaaac aataatacga gctccaaaaa
     5701 tatcgttgag aatgctattt actggataac cttcactacg ctgtttaaaa cgctcgattt
     5761 tatctaaaat cgattctgag gtttttactc ggtaaaaata agaaattccc tctaaatctg
     5821 cacgatataa ataatcatta atagattcgt gtaaattaag acgataagct aaaatatgtg
     5881 gcgtaggcac tttggcaaaa gtatgcttaa gattcacttt ttcaatctta cctgtttcaa
     5941 agtagtcttt cgaaaaagct aagtgcaact tgttgatttc atcaatgagt ttttctattt
     6001 tctcaagcat gctattcact ctctaattta ttatcttgcc ccaaatccag ttagcattgt
     6061 tttcaatgtt ttaaaaataa ttaaaatatc aagccataaa gaaaaatgct taatatagta
     6121 gaagtcatgt tctactttta cttttgtatc atcaacatct gccgcataac cttggacgac
     6181 ctgagcccaa ccggaaatac ccggtttaac gatatggcga taattataga atggaatttc
     6241 ctcttcaaat ttatctacaa aggctgtctg ttctgggcga ggaccaatta agctcatatc
     6301 accttttaat acattaaaaa actgaggtag ctcatcaata cgagttttac ggataaattt
     6361 cccaatacga gttaccctca tatccgcact cgaagcaaac tgtgcatcat ctttttcgga
     6421 gtctttacac atactacgaa atttataaat attaaactcc tttccacctt gcccaacgcg
     6481 tttttgaata aacattgctc caccttcact ttctaagcga atagcaatag cggtgattgt
     6541 cataataggt aaagtaatgg gaaacgtagc aacaatcaat gcaatatcaa tgatacgctt
     6601 cactaatgag taaattggag aaggaagtaa tgaacctaag tcattttcat acatatggcg
     6661 aattttgaca cgcccggtta aagattcctc aagctggcga acgttatata caggaatacc
     6721 attcaatgta catttcgcta aaaatttatg ccattctgcg gacaaatcag gatagtgtaa
     6781 atctaccgca atagagttaa ttctcttttc ccccaatatt ggttcactta attgaatcca
     6841 ctctacattc tgaattcggt tcaaattgat agctcttccg aatggaacca aagcgatttt
     6901 agggataatc caacttcttg aaagaaaata accaataaaa caaaatagaa ttgtgagtat
     6961 aaagctcgta cctagcaatg tattagaata atcaaatcta aatattgtta acacgattaa
     7021 taaaaccgca taccaagaaa taacggtgga aaaaacaaaa cttgctgact tattacctgg
     7081 atatttcatt aacactctta agctgatagc tatcattaca aaggcaagag aagtcgctat
     7141 aatcgtatta gaacgagttt caatagaatt aaatttctct ccccaaaaat agatagcggg
     7201 taagacactt gagaacataa cgcccaaaat actttgaaca gctgtactaa aaaagatacg
     7261 ttcataccaa cgagagtgtc ttttcttttg ctgaatgctc ataatttatc cttttcttaa
     7321 tttcttataa agtcttccta aaagagatct aggcaataac cgtggtaagg ctctaagtat
     7381 aaaaaacatg cttgatgaga ttattgattg taatcccaac tcttttttca attttgctaa
     7441 ttggtactca ctcttaatgt aattccaccc tttacgccga gcgtacattg cagaacctga
     7501 acgaacattt accagtacat ctggtaaatt atacacctca tatttctgtg taataactcg
     7561 taaccataaa ttgtaatctt ccatataaag atgatgctga tagcctccaa catgctcgat
     7621 cacagatttt ttataggcta ctgccatatg gttaaaagga tttcttagta atgctgaaat
     7681 acgaatttca tcatcattaa tgggcacttg ttttataccc agagaattag acattgtttc
     7741 gtcaaattct tcaacttgtc cccccaataa aaccacatca ggatttttac taataaaatc
     7801 aatttgcttt tcaaatcgag atggtaagca aatatcatca gtatccattc taaacaccca
     7861 ctcattagag cagtgtttta agccttcatt taaggccggc ctaaacccac attttgtggt
     7921 aaactaacaa caacaagcgg taatttttca gtaaaatgtt gcacaattcg ctctaaatct
     7981 ccaggaatta taccatcaaa aaccacaaca atttcacttg ctggtaaagt ttgggatact
     8041 aaagaatcta aacacatctc taaataagct ggtttttctt tataatacaa agatattaat
     8101 atcgaaaact tcaca
//
LOCUS       DANLXS010000104         8064 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.104, whole genome shotgun sequence.
ACCESSION   DANLXS010000104 DANLXS010000000
VERSION     DANLXS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.104"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            1..525
                     /gene="proQ"
                     /locus_tag="PXD71_001737"
     CDS             1..525
                     /gene="proQ"
                     /locus_tag="PXD71_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDL6291836.1"
                     /translation="MEQKVEQTQNKTNPSTKEVIAYLAEKFPLCFTLEGEVKPLKVGL
                     FQELAEALKDDEKISKTLLRQVLRNYTNSWRYLAASQANVARVGLQGEEVGVVTEDEA
                     THAAEALAAAKAVVAERKAQERKAQRKEYFKQKAREENAKKRAEFKAKKAIDNLPKAS
                     EEKLAALADKFGRK"
     gene            611..2623
                     /gene="prc"
                     /locus_tag="PXD71_001738"
     CDS             611..2623
                     /gene="prc"
                     /locus_tag="PXD71_001738"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDL6291837.1"
                     /translation="MKLTRLNKIFALALGLMIGNAFAVQPDIKESAIVMPKPNEQHSL
                     STKRVTARLTQSHYHKFQLNDEFAGKIFNRYVDWLDPSHNTFLQADIDEMRSKYATTL
                     DDDLYAGKLDAAFAMYELMSKRRYERYSYALSLLDKEPDLKGNDEIEIDREKAAFPKS
                     VEEANTLWEQRVKNDIINLYLKDKKWPEIKKTLTKRYNFAIKRLTQVKADDILQTYLN
                     SFSREIDPHTSYLSPRAAKAFQESMNLSLEGIGATLTQEDDVTSIRALVPGAPAAKSK
                     KLVVGDKIVGVGQGERGEIEDVIGWRLDNVVDKIKGKKGTKVRLEIEPEKGGKTKIIT
                     LVRDKVRIEDSAAKLTVEKVDGKNVGVIKIPTFYIGLTSDVRKLLTQIEKKNVEGLII
                     DLRENGGGSLTEVIELTGLFIKDGPVVQVQDAFNRIKVHEDPESKSVYDGKVMVMINR
                     HSASASEIFAAAMQDYNRAIIVGQQTFGKGTVQQSRSLNFVYDLDQEPLGFIQYTIQK
                     FYRINGGSTQLKGVEADIKFPEIINAAKTGESFEDNALPWDKIPAANYKEAGNARNAV
                     AALTAKHQERIAKNPEFIVLNENIAIRKERDERKFLSLNLEERLKENKADESKRLKDI
                     NARFAREGKKALKDIDDLPKDYEAPDFFLKEAEQMMLDWSEFDKKS"
     gene            2741..4246
                     /locus_tag="PXD71_001739"
     CDS             2741..4246
                     /locus_tag="PXD71_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HDL6291838.1"
                     /translation="MVAFSGMALAETATVAQVASEPVVQDNILPQLSFAAQQARAAEL
                     VKQADYALEQEQIRQEQARKFAEAEARIKQEIGNNALLLSQATTKVYLDSEFATMWND
                     KAAEKAFLKEYALFAVSGVSSKSAKALQQILNQPEGLGRDILLTDGFLDYLYYNKNVF
                     KNANNWLYNLGSYVPKSPSSEQIFQWTNAVREGDSGQFVTDLVPRNHLYRDTAQRVLA
                     MAGGSSAPKAKKGKAVTEVAGGGNGASFYKLALNAQRLRIIPSFNNGIFVNIPSYQLY
                     YFRDGNLALQSKVIVGREDRRTPVMYSKLSNVVVNPPWNVPTSIMTKDIIPKLARDPG
                     YADRASFEILDSSGNKINPRSVNWSQYLNAKSSPYRIRQKAGDDSALGRYKFNMPSSD
                     AIYLHDTPNRGLFGKTGRALSSGCVRVEKSDELATILLKEAGWGSDKKNQVLGSKKTT
                     SAQIRSDNPVYLYYVTAWVEGGKVQTLPDIYNFDAKIPQHSVNWGKLKSII"
     gene            4416..4970
                     /locus_tag="PXD71_001740"
     CDS             4416..4970
                     /locus_tag="PXD71_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDL6291839.1"
                     /translation="MKQINVQRRKWLSLGGLVLGASLLPGQVMAALSTPAPAALRFRN
                     VNTGDTYTAKFGAGGLNKTDLSQLNYLMRDRHINQVKAIDPKLFVKLNQLQRRLGFHN
                     AEILVLSGYRSAQTNARLRRRSRGVASHSYHILGQAVDFQVSGVPLYKVKQAAESLNN
                     GGVGYYPRSNFVHVDTGPVRTWRG"
     gene            4987..5616
                     /locus_tag="PXD71_001741"
     CDS             4987..5616
                     /locus_tag="PXD71_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6291840.1"
                     /translation="MFNLQIIPVSPFQQNCTVIWDENKNAAIIDGGDNAEGIIQFVES
                     QQLKVQKLLITHGHLDHIMAVEKLREHFQVEVFGSHLADKPLFERLPEMCQQFGLPPV
                     NGFLPDHWLEEGDRVEVGELSYTVRHLPGHAPGHIGFFDFDNRIAFSGDVLFQNSIGR
                     TDLYLGNHSQLLETIREKMFDLDDDFIIIPGHGPHTTIGREKQTNPFLK"
     gene            complement(5651..6022)
                     /locus_tag="PXD71_001742"
     CDS             complement(5651..6022)
                     /locus_tag="PXD71_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDL6291841.1"
                     /translation="MRISYFAFAIFSIFLTACGSKPVSYLPTGSEPIVNIESDIAEKI
                     ELETNSKQFSTTNLTEFPLNLTYKLFWYNKDGVTQSHNGISDSIGWQHLWLQPKQKQT
                     IALIKPTEESHNYRLYLRGVK"
     gene            complement(6025..6384)
                     /locus_tag="PXD71_001743"
     CDS             complement(6025..6384)
                     /locus_tag="PXD71_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDL6291842.1"
                     /translation="MTTEYQKTIFTKIINKEIPANIVYQDELVTAFRDIAPQAPTHIL
                     IIPNKFIPTVNHVSNEDELALGRLFTVAAKLAQEEGIAEEGYRLVMNCNKNGGQEVFH
                     IHMHLLGGQKLGPLVAK"
     gene            complement(6397..6996)
                     /gene="rnhB"
                     /locus_tag="PXD71_001744"
     CDS             complement(6397..6996)
                     /gene="rnhB"
                     /locus_tag="PXD71_001744"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL6291843.1"
                     /translation="MMNEFIYPNARLIAGVDEVGRGPLVGAVVTAAVILDPNNPIQGL
                     TDSKKLSEKKRQALAEEIKAKALSWSLGRAEPTEIDELNILNATMLAMQRAVAGLHIQ
                     PDFVLIDGNRPPKLPMPAQAVVKGDSLVAEISAASIIAKVARDQEMDELDQQFPQYGF
                     AQHKGYPTKLHFEKLAEFGATPYHRKSFAPVRKVLGLID"
     gene            complement(6993..8030)
                     /gene="rsgA"
                     /locus_tag="PXD71_001745"
     CDS             complement(6993..8030)
                     /gene="rsgA"
                     /locus_tag="PXD71_001745"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDL6291844.1"
                     /translation="MSKPKLTHNQQRRIQSNHHKRINKKEIEWQDEMLGEMQQGIVVT
                     RHVKHADVETAEGQIFRCNIRRTLKNVVVGDQVSWRKGSEQLQGISGVIEAVFPRKNE
                     LTRPDYYDGIKVMASNIDQIIIVSAVLPTLSLNIIDRYLVICETANIPALIVLNKIDL
                     LNESERQAVKEQLKIYEEIGYETLCLSADTGENMQALHQYLAKGTSIFVGQSGVGKSS
                     LINQLLPEVNALTGSVSDNSGLGQHTTTASRLYHLPQGGQLIDSPGIREFGLWHLEPE
                     QITLGYREFQSVLGTCKFRDCKHKTDPGCALRQEVENGEISAIRFENYHRLIESLSET
                     KSQRHFRKEEV"
BASE COUNT         2466 a         1445 c         1756 g         2397 t
ORIGIN      
        1 attgagcaaa aagtagaaca aactcagaat aaaaccaatc caagtaccaa ggaagtgatt
       61 gcttatttag cggagaaatt tccgctttgt ttcacgcttg aaggtgaagt aaaaccattg
      121 aaagttgggt tgtttcagga attagctgaa gcattaaaag atgatgagaa aatcagtaaa
      181 acgttattgc gccaagtatt gcggaattac actaattctt ggcgttactt ggctgccagc
      241 caagctaatg ttgctcgtgt cggcttacag ggtgaagaag ttggcgtggt aacagaagac
      301 gaagctactc acgccgcaga agctttggct gctgcgaaag cggtggttgc agaacgtaaa
      361 gcacaagagc gtaaagcaca acgtaaagag tacttcaaac agaaagcacg tgaagaaaat
      421 gcgaaaaagc gtgcagaatt taaggcgaaa aaagcaatag ataatttacc taaagcttca
      481 gaagaaaaat tagctgcatt agcagataaa ttcggtcgta aatagttttc aagtcgcttt
      541 gtgcttacaa gcggtcggat tttagtaaaa ttttgcaaaa aaatgaccgc ttgattattt
      601 ggaaaagaag atgaaattaa caagattaaa taaaattttt gccctagccc taggtttaat
      661 gataggaaac gcttttgccg ttcagccgga cattaaagaa agtgcgattg tgatgccaaa
      721 acctaatgag cagcatagct tatcaaccaa gcgtgtaaca gcccgattaa ctcaatcgca
      781 ttatcataaa tttcaactga atgatgagtt tgcagggaag atttttaacc gctatgtcga
      841 ttggcttgat ccttctcata atacttttct tcaagccgat attgatgaaa tgcgtagtaa
      901 atatgctaca acattagatg atgatttata tgctggtaaa ttagatgcag catttgcaat
      961 gtatgagtta atgtctaaac gtcgttatga gcgttatagc tatgccttat ctttactaga
     1021 taaagagcct gacttaaaag ggaatgatga aattgagatt gaccgtgaga aggccgcatt
     1081 tccaaaaagt gtagaagagg caaatacact ttgggagcag cgagttaaaa acgatattat
     1141 caatttatat cttaaagata aaaaatggcc tgaaattaag aaaacactca ctaaacgtta
     1201 taactttgcg attaagcgtt taactcaagt taaggctgat gatattttac aaacttactt
     1261 aaattcattc tctcgtgaga tcgatcccca cactagctat ttgtctcctc gtgcggcaaa
     1321 agcgttccaa gagagcatga atctgtcgct agaaggaatt ggtgcaacgc taacccaaga
     1381 agatgatgta accagcatta gagcattagt gccaggagcc cctgccgcta aaagtaagaa
     1441 gctggtagtc ggcgataaaa ttgtcggtgt aggtcaaggt gagagaggtg agattgaaga
     1501 tgttatcggt tggcgtttgg ataatgtagt cgataaaatc aaaggtaaaa aaggcactaa
     1561 agttcgttta gaaattgagc ctgaaaaagg tggaaaaact aaaattatta cccttgtgcg
     1621 tgataaagtt cgtattgagg atagtgctgc taaattaacg gtagaaaaag tagatggaaa
     1681 aaatgtaggg gtaattaaga tccctacttt ttatatcggt ttgacttcag atgtacgtaa
     1741 attacttact caaatagaga aaaaaaatgt tgaaggattg atcattgatt tacgtgaaaa
     1801 cggtggtggt tcgttaactg aagtgattga attaactggt ttatttatta aagatgggcc
     1861 tgtagtgcaa gtacaagacg cattcaatcg tatcaaagta cacgaagatc cagaatcaaa
     1921 atcagtttat gatggaaaag tgatggtgat gattaatcgc catagtgctt cagcctctga
     1981 aatcttcgct gcggcaatgc aagattacaa ccgtgcaatt attgtagggc aacaaacctt
     2041 tggtaaggga actgttcaac agagccgttc attgaatttt gtatatgatt tggatcaaga
     2101 gcctcttggt tttatccaat atacgattca aaaattctac cgtatcaatg gtggaagtac
     2161 ccagctaaaa ggggttgagg cggatattaa attcccggaa atcattaatg ctgcaaaaac
     2221 aggtgaaagt tttgaagata atgcgcttcc ttgggataaa attccagcag ctaattataa
     2281 agaggctggg aatgctcgta acgcggttgc tgcattaaca gcgaaacatc aagagcgtat
     2341 tgctaaaaac ccagagttta tagtattaaa cgagaatatt gcgattcgaa aagaacgtga
     2401 tgagcgtaag tttttatcgt taaatttaga agaacgcctg aaagaaaata aagcagatga
     2461 aagcaagcgc ttaaaagata ttaatgctcg ttttgcccgt gaaggcaaaa aagcattaaa
     2521 agatattgat gatttaccaa aagactatga agcaccggac tttttcttaa aagaagctga
     2581 acagatgatg cttgattggt cagaatttga taaaaaatca taaatgtgtt tataatgcgc
     2641 acataacgta ccgcttagct ccgctaagcg gtttattttt ttataaattt gcaaatatta
     2701 agaggaagag tgggtgaaaa cctttaaatt attacctatt atggtggcgt tttctggcat
     2761 ggctttggct gaaacagcca cagttgctca ggtggcttct gagcctgttg tacaagataa
     2821 tattttacca caattaagtt ttgcagctca gcaggctcgt gctgcagaat tggtaaaaca
     2881 ggccgattat gcgttagaac aggaacaaat tcgtcaagag caagcaagaa aatttgcaga
     2941 agcggaagca agaattaagc aagaaatagg taataatgcc ttattgctga gccaagcaac
     3001 aactaaggtt tatttagaca gtgaatttgc gacaatgtgg aatgataaag ccgcagaaaa
     3061 agcatttttg aaagaatatg cattatttgc agtaagtggg gtatcctcta aatcagctaa
     3121 ggcattgcag caaatcttaa atcaaccgga aggtttaggt agagatattt tattaactga
     3181 tggcttctta gattatttgt attacaacaa aaatgtgttt aaaaatgcaa ataattggtt
     3241 atataactta ggcagttatg taccaaaatc gccctcttca gagcaaatct tccaatggac
     3301 taatgccgtg agggaaggcg attctgggca gtttgtaaca gaccttgttc caagaaacca
     3361 tttatatcgt gatacggcac agagagtatt ggcaatggca ggaggctctt cagcacctaa
     3421 agccaaaaaa ggtaaagctg ttacggaggt agctggtggc ggtaatgggg cttctttcta
     3481 taaattagcg ctcaatgctc aacgtttacg tattattcca agttttaaca atggaatttt
     3541 tgtgaatatc ccaagctatc aattatatta tttccgtgat ggtaatttag ctcttcaatc
     3601 taaagtgatt gttgggcgag aagatcgccg tacaccggta atgtatagta agctgagcaa
     3661 tgtggtagtg aatccacctt ggaatgtacc gactagtatt atgactaaag atattattcc
     3721 taaactagcg cgtgacccag gttatgcaga tcgtgctagt ttcgaaattc ttgatagtag
     3781 tggcaataaa attaatccgc gtagcgtaaa ttggtcacaa tatttaaatg ctaaaagttc
     3841 accataccgt attcgccaga aagcaggtga tgatagcgct ttaggacgtt ataaatttaa
     3901 tatgccaagt tcggatgcaa tttatttgca tgatacgcca aatagaggat tatttggtaa
     3961 aacggggcgt gcgttaagtt ctggttgtgt ccgtgtagaa aaatctgatg aattggcaac
     4021 cattttatta aaagaagcgg gttggggatc tgataaaaag aatcaagtct taggcagtaa
     4081 gaagacgacc tcggctcaga ttcgttcaga taacccggtt tatctctatt atgtgacggc
     4141 ttgggttgaa ggtggaaaag tgcaaaccct tccggacatt tataactttg atgccaaaat
     4201 cccacaacat tctgtgaatt ggggaaaatt aaagagcatt atttaatcgt gctatttctg
     4261 ataaattcca agaagatatc atcttcttgg aactttttga aaatatagct tacataaatt
     4321 tacgattgta aatttttact ttaagtatgg tgtatagaaa ttcgctcatt tgtgagtgat
     4381 tttctataaa tcattagcta acctatgagg aaaaaatgaa acaaattaat gttcaacgcc
     4441 gtaagtggct atcactcggc ggacttgttt taggggctag ccttctgccg ggtcaagtaa
     4501 tggcagcgct ttctactcct gccccagctg cgttacgttt tcgtaatgtg aatacaggcg
     4561 atacttacac tgctaaattt ggtgcaggtg gattaaataa aaccgattta agccagttaa
     4621 actatttaat gcgggatcgt catatcaatc aagttaaagc aattgatcca aaattatttg
     4681 tcaaattgaa tcaattacaa cgccgtttag gtttccataa tgcggaaatc ctagtattaa
     4741 gtggctatcg ctccgcacaa acaaatgcaa gacttcgtcg tagaagccgt ggtgttgcca
     4801 gccatagtta ccatattctt gggcaagcag tagatttcca agtatcaggt gtgccgttat
     4861 ataaagtaaa acaagcggct gagagcttga ataatggtgg cgtaggttat tatcctcgta
     4921 gtaactttgt tcacgtagat actggcccag ttcgcacttg gagaggctaa caagcggtct
     4981 tattttatgt ttaatttgca aattattcct gttagtccat ttcaacaaaa ttgcacggtg
     5041 atttgggacg aaaataagaa tgcggctatt attgatggcg gcgataatgc cgaaggcatt
     5101 attcaatttg ttgaatctca gcagcttaag gttcagaaat tattgattac gcacggtcat
     5161 ttagatcata ttatggcggt ggaaaagttg agagagcatt ttcaagtaga ggttttcggc
     5221 tcgcatcttg cagataaacc tttatttgaa cgtttgccgg aaatgtgcca acaatttgga
     5281 ttaccaccag taaatggctt tttgccggat cattggttgg aggaaggtga tcgggtcgaa
     5341 gtcggcgagc tgagctatac cgttcgacat ttgccgggtc atgctccggg tcatatcggt
     5401 ttttttgatt ttgacaatcg tattgctttt agtggcgatg ttttattcca aaatagcata
     5461 ggtcgaacag atctctattt aggcaaccat agccagttat tggaaacgat cagggaaaaa
     5521 atgtttgatt tagatgatga tttcatcatc ataccgggac atggtccaca cacaactatc
     5581 gggcgggaaa aacagaccaa cccgtttttg aaataaataa agaagcagag cagttggctc
     5641 tgcttttttg ctattttacg cctcgtaaat agaggcggta attatggctt tcttccgtcg
     5701 gtttaatgag tgcaatggtc tgtttttgct taggttgcaa ccatagatgt tgccagccga
     5761 tactatctga tataccgtta tgggattgtg ttaccccatc tttattatac caaaataatt
     5821 tataagtaag attaagcgga aattcagtta aatttgtggt gctaaattgc ttgctattcg
     5881 tttccagctc tatcttttca gcaatgtcag actcaatatt gacaataggc tctgagccgg
     5941 ttggtaaata gctgacaggt ttgctaccac aagcggtcaa aaaaatcgaa aaaattgcaa
     6001 atgcaaagta ggaaatacgc atagttattt tgccactaaa ggtcctagtt tttgtccgcc
     6061 taacaagtgc atatgaatat ggaacacttc ctgcccgccg tttttattac aattcatcac
     6121 taagcgatag ccttcttcgg caatgccttc ttcttgggct aattttgccg ccaccgtaaa
     6181 taaacgccct agggctaatt catcttcatt actcacgtga ttgacggttg gaataaattt
     6241 attcggaata atcaaaatat gcgtgggtgc ttgtggagca atatcacgaa atgcggtcac
     6301 aagttcatct tgatagacaa tatttgccgg aatttcttta ttgatgattt tggtgaaaat
     6361 cgttttttgg tactctgtag tcattgtgtt ttccttctaa tcgattaaac ctaatacttt
     6421 tcttacaggg gcaaaactct tacgatgata aggtgttgcc ccaaattctg ctaatttttc
     6481 aaaatggagt ttagtcgggt agcctttgtg ctgtgcgaag ccgtattgcg gaaattgttg
     6541 gtctaattcg tccatttctt gatctcgggc gactttggca ataatcgaag cagcactgat
     6601 ttcagccact aagctgtctc ctttcaccac cgcttgtgcc ggcattggca gttttggcgg
     6661 acgattgccg tcaattagca caaaatccgg ctgaatatgc aagccggcaa ccgcacgttg
     6721 cattgccaac attgtcgcat ttaaaatatt cagctcatca atttctgtcg gctcggcacg
     6781 tcctagcgac cagctcagag ccttggcttt aatctcttct gccaaagctt ggcgtttttt
     6841 ctcggataat tttttggaat cggttaagcc ctgaatcggg ttattcggat ctaaaatcac
     6901 ggctgcggtc acaaccgctc cgactaaagg tcctcgtccg acttcgtcaa caccggcgat
     6961 taatcgggcg ttagggtaaa taaattcatt cattatactt cttcttttct gaaatggcgt
     7021 tggcttttgg tttcagacag gctttcaatt aagcgatggt aattttcaaa ccgaattgca
     7081 gaaatttcgc cattttcgac ctcttgtctt aaggcacaac ccgggtcggt tttgtgtttg
     7141 caatcacgga atttgcacgt gcctaaaacc gattgaaatt ctcgatagcc taaggtgatt
     7201 tgctcaggtt ctaaatgcca taagccaaat tctcgaatgc ccggcgagtc aatcagctgt
     7261 ccgccttgcg gtaaatggta taagcgagag gcagtggtgg tgtgctgacc taaccctgag
     7321 ttatcgctca ctgagccagt gagagcattg acttcaggta acagttggtt aattaggctg
     7381 gatttcccca cgccggattg tccgacaaaa atagacgtac ctttagcaag atattggtgt
     7441 aacgcctgca tattttcacc cgtatcagcc gataaacata gggtttcata gccaatctct
     7501 tcatagattt tgagctgctc tttaactgct tgccgttcgc tttcgtttaa taaatcaatt
     7561 ttgtttagca cgattaaggc aggaatattc gccgtttcgc aaatcactaa ataacggtcg
     7621 ataatattca gcgaaagtgt aggtagcaca gctgatacaa taatgatttg atcgatgttc
     7681 gatgccatca ccttaatacc gtcataataa tcggggcggg taagttcatt tttacgtggg
     7741 aagacggctt caatgacccc actaatccct tgtagttgct cactaccttt acgccaagaa
     7801 acctgatcgc ccaccactac atttttcaat gtacggcgga tattacagcg gaaaatttgc
     7861 ccctctgcgg tttcaacatc agcgtgttta acgtggcgag taacgacgat gccctgttgc
     7921 atttctccta acatttcatc ttgccattca atctcttttt tgttgatacg cttatgatgg
     7981 ttagattgga ttctacgctg ttggttatgg gttaatttcg gtttactcaa aagaactctc
     8041 ggtaattatt gcaaatgaaa aata
//
LOCUS       DANLXS010000105         7892 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.105, whole genome shotgun sequence.
ACCESSION   DANLXS010000105 DANLXS010000000
VERSION     DANLXS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.105"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            63..4259
                     /locus_tag="PXD71_001746"
     CDS             63..4259
                     /locus_tag="PXD71_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6291845.1"
                     /translation="MKTKTFTRSYLASFVTIVLSLPAVASVVRNDVDYQYFRDFAENK
                     GPFSVGSMNIDIKDNNGQLVGTMLHNLPMVDFSAMVRGGYSTLIAPQYLVSVAHNTGY
                     KNVQFGAAGYNPDSHHYTYKIVDRNDYEKVQGGLHPDYHTPRLNKLVTEVVPAAVTNA
                     GTSIKPYLNEERFPMFLRAGSGTQALRGKESNKTTGIAGAYEYLTGGTTLQLSKSSPD
                     HWLDYSSNLYQVSYGPLSTYALPGDSGSGSYAYDMNEKRWVLVGVLNFYNGMDNQFNR
                     SAIIRKDFHEKKFAEDIAGTINNTVQNAQFNWTAQGKSSSLSQSNNVQKLNVDLKDSS
                     IANQNTSLPQENHGKTINFNGKDATIVLKQDIDQGAGALNLNANLTIRPETDQTWQGA
                     GIIVGKDKKVNWQVKNPQGDRLSKLGEGTLYVNGRGQNLGDISVGDGIVILNQQADHQ
                     GRKQAFNTVGIVSGRPTVVLGSADQVNPDNIYFGFRGGRLDLNGNSIAFKRIQNSDKH
                     ARIVNHNRDHISTLIIQGQDPLTSNDLIWGKWASNSPADIYEYTNPYQNKRKDYFRLK
                     GNSRVYYPTNATSNDHWEFLSSNREQAIQKILDAKNLRQRYDTFNGFIGEDASNKTNG
                     ILNVVFDTKTEVNTEQDKLKNIYTMSGGFNLNGELTLKGGTLLLSGHPTPHAYDIKNK
                     HDVVRENDWQDSHFTAKNITVNKMAQLYIGRNVNEVNSHFTATDKAKLNLGFINRSTP
                     SCYDSEYTGTTHCEVQAVISDNIFANLATTAIKGNVKLQNHSQLNLGKANLTGSVQAD
                     QTTHITLANHSHWLNNGTSQIGHLTMEKGSILSLNDKFATTEIPVRFNKMIIQGNLKG
                     NGRINYTANLAKGESDHLQVDGIAEGNFVLAVRNSTTEANPKSSLNLLSLKNSNQEGN
                     KASISLENNYVDLGTYRYVLENRNHNYHLFNPLIPNSTSKEMNATSVSSIPKKESVTN
                     VPTLDKKETEQNLTQLQKDFSAHQLENQKAKQSMINAQSELRRLNSQLNVLQKYVNSR
                     RLGYYTQQAVLEQISIIQNKIKQTQTIFNDANATVKLTDQKLEEAKLALGSVNDLVLI
                     KASAPAMQATNQDTSMMNIIQADWISQYANTALSELSAQANSALQISNSLDRQLFKQS
                     DKFNVWSSVEHQKTEHKSDLYRPYKQQTNLTQLGIQMPIDNGLMFGVALSKNHANAEF
                     NEGVNGKSNLLMASLYGKWQSQQGTFISLDGSYGKAKNQLYLFGENHFTRRISSIGAN
                     IGHQFDLAGVQIQPTIGARYYHFSGQDYTLGGAKISSPNTHFMTYQAGLKASKTFSLD
                     DWKVEPSITTHYVDASNKRLAVNVNNNNINQRFGRYLKTEAGIRFEHNQWQFDLHAGF
                     INGNEIKKQHFAGVKLGYSW"
     gene            complement(4314..5408)
                     /gene="mobA"
                     /locus_tag="PXD71_001747"
     CDS             complement(4314..5408)
                     /gene="mobA"
                     /locus_tag="PXD71_001747"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL6291846.1"
                     /translation="MSKTIEERISAVILAGGLARRMNGVEKGLQLFEGKPLISHILAR
                     LSPQVSDIWLNVNRSIEQYQQLYPAFSYYQDSLPDFQGPLSGMLAGFEQIESDYLLFV
                     PCDTPFMPELLLQKLKTALRINNVQIAYAHDGERPHPTFALIHRSVQEDLKAYLGSNQ
                     QQLLAFFQSQKSVAVDFSEQKSAFTNVNTLEDLSRVSQFPVKNLAITGYSGTGKTTLL
                     EKLIPKLTACGIRVGLIKHSHHNVDVDKKGKDSYRLREAGANPTMIVCDERWALMTET
                     KQAVEFSQLIAKFNPQEVDLVFVEGFKHETLPKIQLHRKGIEKPLPELDQWTIATATD
                     YPLERENWLDINNIGGIADFIKNWLENKAS"
     gene            5495..5764
                     /locus_tag="PXD71_001748"
     CDS             5495..5764
                     /locus_tag="PXD71_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HDL6291847.1"
                     /translation="MKCERLEELLELLGEHWRKNPDLHLIDILQQLSVEVGEPDNFKA
                     LSDEVLIYQLKMRNAGKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            5828..6466
                     /locus_tag="PXD71_001749"
     CDS             5828..6466
                     /locus_tag="PXD71_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL6291848.1"
                     /translation="MKKLALKTAFVALSALFTFNSTAIAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELSYKIPSQIKEKLPSDSKLVQYHVNFLGRQSEDLTRAWAL
                     AMALGAEDKVKTALFEGAQKDAFKSMDDIRSVFLANGITAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFADSSSTNDFIKRYVDAVVFLSKK"
     gene            6550..7326
                     /locus_tag="PXD71_001750"
     CDS             6550..7326
                     /locus_tag="PXD71_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL6291849.1"
                     /translation="MTKLSILNLVPIREGQTAKQAVDSMVKLAKHAESIGIERYWIAE
                     HHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLPNHSPYIVAEQYGTLETLYPNRV
                     ELGLGRAPGTDMRTATALRRNAQSLPFPDEISELKGYFEGTNSVSAYPAQGLNVPFYI
                     LGSSPESAYLAAERGLPYAFASHFAPRFMDDAVRIYRTHFKPSEVLDKPYVILGVNAI
                     AAETDQEAEQLATTQTQFFLNVVTNAQNYLQPPVESDDKV"
     gene            7336..7590
                     /locus_tag="PXD71_001751"
     CDS             7336..7590
                     /locus_tag="PXD71_001751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase"
                     /protein_id="HDL6291850.1"
                     /translation="MKAEIVPHFGPVSFQQNALYNQEKPVVRQMTACSLIGSRESIEQ
                     QLKSLKARIEIDEIMAVSYIFDEQKQHQSYTWLKEIADKV"
     gene            7592..7885
                     /locus_tag="PXD71_001752"
     CDS             7592..7885
                     /locus_tag="PXD71_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL6291851.1"
                     /translation="MSKTMQQKAADAHNMCKLKGDSMLDNSDRQIAFEAVYDSEEIAV
                     QAVEFFTQKAKSVETEPCDIQSEITPIIDGFLMKMWITFSCQAEVILFQMAVR"
BASE COUNT         2639 a         1560 c         1484 g         2209 t
ORIGIN      
        1 tatcagttat aagacagaat agccagaatt ttttattact tataaggata aacttctcat
       61 ttatgaagac caaaacattt actcgttctt atcttgcttc ttttgtaaca atcgtattaa
      121 gtttacctgc tgtagcatct gttgtacgta atgatgtgga ctatcaatac ttccgcgatt
      181 ttgccgaaaa taaaggacca ttttcagttg gttcaatgaa tattgatatt aaagacaaca
      241 atggacaact tgtaggcacg atgcttcata atttaccaat ggttgatttt agtgctatgg
      301 taagaggtgg atattctact ttaattgcac cacaatattt agttagtgtt gcacataata
      361 ctggatataa aaatgttcaa tttggtgctg caggttataa ccctgattca catcactata
      421 cttataaaat tgttgaccgc aatgattatg aaaaggttca aggagggttg cacccagact
      481 atcatactcc tcgattaaat aaattagtaa cagaagttgt gcctgccgca gtcaccaatg
      541 caggtacatc tattaaaccc tacttaaatg aagaacgctt ccctatgttt cttcgtgctg
      601 gttcagggac acaagcgcta agaggaaaag aaagtaataa aacaactgga atcgctggtg
      661 cttatgaata tcttactggc ggtaccacat tacaattatc taaaagctcc cctgatcact
      721 ggttagatta ttcaagtaac ctttatcaag taagctatgg accactttca acctatgcac
      781 tacctggtga tagtggttca ggttcttacg cctatgatat gaacgaaaaa cgatgggtat
      841 tagttggtgt gctcaatttc tataatggta tggataatca attcaaccgc tctgcgatta
      901 tccgtaaaga tttccacgag aaaaaatttg ccgaagatat tgcaggaaca atcaataata
      961 ccgtacaaaa tgcacaattc aattggactg ctcaaggtaa atccagctct cttagtcaat
     1021 caaataatgt gcaaaaactc aacgttgatc taaaagatag tagcattgca aaccaaaaca
     1081 cttctctgcc acaagaaaat cacggtaaaa ccattaattt taatggtaaa gatgcaacta
     1141 ttgtactaaa acaggatatt gaccaaggtg caggtgcatt aaatctgaac gctaatctca
     1201 ctattcgtcc tgaaacagac caaacttggc aaggtgcagg tattatcgtc ggtaaagata
     1261 aaaaagtgaa ttggcaagta aaaaatccac aaggcgatcg tttatctaaa ctcggggaag
     1321 gaacactcta tgtaaatgga cgtggacaga atcttggcga tatcagtgta ggtgatggta
     1381 ttgtaatact taaccagcaa gccgatcacc aaggaagaaa acaggccttt aatacagtag
     1441 gaatcgtaag tggtcgcccc actgttgtgc taggtagtgc agatcaagtt aatcccgata
     1501 atatttactt tggatttcgc ggaggtcgtt tagacctaaa cggtaacagc atcgccttta
     1561 aacgtattca aaacagcgat aaacatgctc gtattgtaaa ccacaatcgc gatcacattt
     1621 ctaccttaat aatacaaggc caagatcctc tcactagtaa tgatcttata tggggaaaat
     1681 gggcaagtaa tagcccagca gacatttacg aatataccaa tccttatcaa aataaacgca
     1741 aagattactt ccgtctgaaa ggtaattcga gagtatatta tccaacgaat gctacaagta
     1801 acgatcactg ggaatttctt tccagtaacc gcgagcaagc aatacagaaa atcctagatg
     1861 ccaaaaactt aagacagcgc tatgacacgt ttaatggttt tataggggaa gatgcttcca
     1921 ataaaactaa tgggatatta aatgtcgtgt ttgatacaaa aacagaagta aatacagaac
     1981 aagataaatt aaagaatatc tacacaatgt cgggaggatt taaccttaat ggtgaactca
     2041 cccttaaagg tggtacattg ttgctttctg gtcacccaac gccacacgct tatgatatta
     2101 agaataagca tgatgttgtg cgtgaaaacg attggcaaga cagccatttt actgctaaaa
     2161 atatcacggt aaataaaatg gcacaactct atatcgggag aaatgtcaat gaagtaaata
     2221 gtcactttac tgcgactgat aaagccaaac tcaatttagg atttattaat cgttcaacgc
     2281 caagttgcta tgattctgaa tacacaggca ctacacattg tgaagtgcaa gcggtcattt
     2341 ccgataatat ttttgcaaat ctagcaacaa ccgccattaa aggtaatgtt aaattacaaa
     2401 accatagcca attaaattta ggcaaagcaa acctcactgg ttctgtacaa gctgatcaaa
     2461 caactcatat cactttagca aatcacagtc actggttaaa caatggtacg agccagattg
     2521 ggcatcttac aatggaaaaa gggtcgatcc ttagcctaaa cgataaattt gctaccacgg
     2581 aaatcccagt ccgattcaac aagatgatca tccaaggtaa tctaaaaggt aatggacgaa
     2641 ttaactatac cgcaaattta gccaagggcg aatctgatca tctccaagtt gacggtattg
     2701 ctgaaggaaa ttttgtcctt gccgttagaa atagcacaac tgaagcaaat ccaaaaagct
     2761 cattaaacct actaagctta aaaaatagca accaagaagg caataaagct tctatttctc
     2821 tagaaaataa ttatgttgat ctaggtactt atcgttatgt attagaaaat cgtaatcaca
     2881 attaccattt atttaatcca ttaataccaa attcaacctc taaagagatg aatgctacat
     2941 ctgtatcctc tattccaaaa aaggaatctg ttactaatgt tcctacttta gataagaaag
     3001 aaactgaaca aaatcttact caactacaaa aagatttttc agcacaccaa ttagaaaatc
     3061 aaaaagcaaa acaatctatg ataaatgctc aatctgagct aagacgactc aattcacaac
     3121 tgaatgtatt gcaaaaatat gtgaattctc gtcgcttagg ttactatact cagcaggcag
     3181 ttttagaaca aattagcatt attcaaaata aaattaaaca aacacaaaca atatttaatg
     3241 acgctaatgc aactgtaaaa ctcacagatc aaaagctaga agaagccaaa ttagctctag
     3301 gctctgtaaa cgatcttgta ttaataaaag cctctgctcc agcaatgcaa gcaactaatc
     3361 aagatacgag tatgatgaat attattcaag cagattggat aagccaatac gctaacacag
     3421 cactttctga actctcggca caggctaatt ctgctctgca aatcagtaat agcttagatc
     3481 gccaactctt caaacaaagc gataaattca acgtatggag cagcgtcgaa catcagaaaa
     3541 ccgagcataa atcagattta taccgcccgt ataaacaaca aaccaacctg acccaactgg
     3601 gcatacaaat gccgatagat aacggtttaa tgtttggagt tgcattatct aaaaaccacg
     3661 ctaacgcgga atttaacgag ggtgtaaacg gtaaatcgaa tctactaatg gcaagcctat
     3721 atggtaagtg gcaatctcaa caaggcactt ttatcagcct tgatggcagc tacggtaaag
     3781 caaaaaacca actctaccta tttggtgaaa accactttac ccgccgaatt tcctctattg
     3841 gtgctaacat tggacatcaa tttgacctcg caggagttca aattcagcca acaataggag
     3901 caagatacta ccatttcagc ggccaagact atacactagg aggagcgaaa atcagctcac
     3961 caaataccca ctttatgaca tatcaagcgg gtctaaaagc tagtaaaacc ttttcattgg
     4021 atgactggaa agttgaacca agcattacaa cccactatgt ggatgcaagt aacaaacgct
     4081 tagcagtaaa cgtcaataat aacaatatca accaacgttt cggtcgctat ctgaaaaccg
     4141 aagctggtat ccgatttgaa cataaccaat ggcaattcga cttacacgct ggtttcatta
     4201 acggcaacga aatcaaaaaa caacattttg ctggtgttaa gctaggatat agctggtaag
     4261 aaaaagcccc tagaactatt tctaggggct ttttctactc ttaaaaagag tatctaactt
     4321 gccttatttt ctaaccaatt cttaataaaa tctgctattc ccccaatatt attaatatcc
     4381 aaccaatttt ctctctcaag cgggtaatcc gttgcggtgg caatcgtcca ttggtctagc
     4441 tctggcaatg gtttttcaat ccctttgcgg tggagctgaa tttttggcaa ggtttcgtgt
     4501 ttaaagcctt ctacgaaaac gagatcaacc tcttgcgggt tgaattttgc aattaattga
     4561 gaaaattcga ccgcttgttt ggtttctgtc attaatgccc aacgttcatc gcacacaatc
     4621 attgtcgggt ttgctcctgc ttcacgtagg cggtagctgt ctttgccttt tttatctaca
     4681 tcaacgttat ggtgggaatg tttaatcagc ccaacccgaa taccgcaagc ggtcaatttt
     4741 gggattaatt tttccaataa ggtggtttta cctgtgccgc tgtagcctgt aattgcaagg
     4801 ttttttacag ggaattggct tactcggctt aaatcttcca atgtattcac gttggtgaat
     4861 gccgattttt gttcggaaaa atcaactgcc acgctttttt gtgattggaa gaacgcaagc
     4921 aattgctgtt ggtttgagcc taaataggct ttcaaatctt cttgtaccga acgatgaatc
     4981 aacgcaaaag tgggatgtgg acgctcacca tcatgggcat atgcaatttg tacgttattt
     5041 atccgcaaag cagtttttaa tttttgcaat aacagctcag gcataaaggg cgtatcgcaa
     5101 ggcacaaaaa gcaaatagtc actttcaatt tgctcaaatc ctgccagcat tccactgagt
     5161 ggaccttgaa aatcaggcaa gctatcttga tagtaagaaa aagcaggata aagctgctga
     5221 tattgctcaa tagaacgatt gacattaagc caaatgtcac taacttgtgg tgaaagtcgg
     5281 gctaaaatgt gtgaaataag cggttttcct tcaaaaagtt gtaacccttt ttctacccca
     5341 ttcattcgac gagctaaacc gcccgctaaa atcacggctg aaatgcgttc ttctattgtt
     5401 ttactcacag ctttatccct taaaattttg caaaaataag agtaaaagta tattatttcg
     5461 tttaatttcc aacaagtttt ttaaggggta aaagatgaaa tgcgaacgtt tagaagagtt
     5521 attagagtta cttggcgaac attggcgtaa aaatcctgac ttacacctca ttgatatttt
     5581 gcagcagctt tcagttgaag tgggcgagcc tgataatttc aaagcgttaa gcgatgaagt
     5641 gttaatctat cagcttaaaa tgcgaaatgc aggcaaattt gagcctattc ccggcataaa
     5701 aaaagattat gaagatgatt ttaaaacggc tttattgcga gctcgtggaa ttttaaacga
     5761 ttaagattgt cttatccgtt agctagttta gcatttatta tttatcaaca tttttgaagg
     5821 aatcattatg aaaaaattgg cattaaaaac ggcatttgtt gcactttctg ccctttttac
     5881 atttaactca acggcaatcg ctgctgatcc aaccgcaggc aaagaatata tcgaagtgcg
     5941 taaagcacct tctgcacaaa aagaagtggt ggaatttttc tcattctact gcccacactg
     6001 ttatgatttt gaattaagct acaaaatccc aagccaaatc aaagaaaaat taccaagcga
     6061 tagcaaatta gtgcaatatc acgttaattt cttaggtcgc caatctgaag atttaactcg
     6121 tgcttgggca ttagcaatgg cattaggtgc ggaagataaa gtaaaaaccg cattatttga
     6181 aggtgctcaa aaagacgcat tcaaatcaat ggacgatatt cgctctgtat tcttagcaaa
     6241 cggcattaca gctgaacagt ttgatagcgg tattaatagc tttgcggtaa atggcttagt
     6301 gaataaacaa gttcaattag cggaagactt ccaaattcgt ggtgtgcctg cattcttcgt
     6361 aaatggtcaa taccaactca acttagaagg ctttgcagat tcttcatcaa cgaatgattt
     6421 catcaaacgt tatgttgatg cggttgtgtt cttaagcaag aaataacccc ttaaaatagt
     6481 tagggttttt acaatgctga gtaggtaaat cctattcagc attttttata tgcaaactcg
     6541 gagaacaaaa tgacaaagtt atctatccta aaccttgtac ctattcgtga agggcaaact
     6601 gccaaacaag cggtcgattc tatggtgaaa ttagcaaaac acgcggaaag tatcggcatt
     6661 gaacgctatt ggattgcaga gcaccacaat atgaaaaacc ttgccagctc ggcaacacag
     6721 ctcctcattc agcatgccct ttctcacact gaaaaattaa gagtgggatc aggtggcgtg
     6781 atgttgccta accatagtcc ttatattgtg gcagagcaat acggtacgct tgaaacactc
     6841 taccctaacc gtgtagaact tgggcttggg cgagcaccag gcaccgatat gcgaaccgct
     6901 accgctttac gccgtaatgc acaaagtctg ccatttcctg atgaaatcag cgaactaaaa
     6961 ggttattttg aaggcacaaa ttctgtgtct gcctaccctg cacaggggtt gaatgtgccg
     7021 ttttatattt taggctcaag ccctgaaagt gcctatttag cagcagaacg tggcttgcct
     7081 tatgcctttg cttcacattt cgctcctcgt tttatggacg atgcggtgcg aatttatcgc
     7141 acacacttta agccgtctga agtgttagat aaaccctatg tgattttagg tgtgaatgcg
     7201 attgcggcag aaaccgacca agaggcagaa cagcttgcta ccactcagac tcagtttttc
     7261 ttaaatgttg ttaccaatgc ccaaaactat ttacaaccgc ccgtcgaatc cgatgataaa
     7321 gtgtgaaaaa attttgtgaa agcggaaatc gtaccgcatt tcgggcctgt ttcgttccaa
     7381 caaaatgcgt tatacaacca agagaaaccc gttgttcgcc aaatgactgc ttgttcactt
     7441 atcggtagcc gagagagcat tgaacaacag ctcaagtcat taaaagcacg cattgaaatt
     7501 gatgaaatta tggcggtaag ctacattttc gatgagcaaa aacagcatca atcctatact
     7561 tggttaaaag agattgcaga taaggtgtga tatgagcaaa acaatgcaac aaaaagccgc
     7621 tgatgcacat aatatgtgta agctaaaagg cgactctatg ttagataact cagaccgcca
     7681 aattgccttt gaagcagtgt atgatagtga agaaatagct gtacaagcgg tcgaattttt
     7741 cacacaaaaa gcaaaatcgg tagaaaccga gccttgcgac attcaaagtg aaatcacacc
     7801 cattatagat ggctttctga tgaaaatgtg gataactttc agttgccaag cagaagtaat
     7861 cttatttcaa atggcggtga gatagatata aa
//
LOCUS       DANLXS010000106         7730 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.106, whole genome shotgun sequence.
ACCESSION   DANLXS010000106 DANLXS010000000
VERSION     DANLXS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.106"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            175..747
                     /locus_tag="PXD71_001753"
     CDS             175..747
                     /locus_tag="PXD71_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL6291852.1"
                     /translation="MSRRSEPKEEMVNRILSATESLIVQEGLQNLSMRSIAKEAGIAS
                     GTLYLYFKTKDDLLHSLAAQLLECYNGYMHFEFKPEMALFEQYKALVNKKWQFLIERL
                     DLVKQFQAVIGIDELIRETIADETSFWNKLILEGKKQRIIADLSNELLYGLSIGTLVD
                     IQYLQNLNQENQFDGYLDEIILRTWKAITF"
     gene            778..1983
                     /locus_tag="PXD71_001754"
     CDS             778..1983
                     /locus_tag="PXD71_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL6291853.1"
                     /translation="MTTETQKLGKGRKFLIVLTLLIVLIVFAAIVGLQKIGAMKKAEA
                     AANMPEAVSEVTAMQVTTQEWTPTISAVGYIRPNQGAMLSAETSGVVSRVLVTSGQRV
                     KKGDLLVEFDSSVEIANLRASEAQLPNAKANLERYRNLIASNSASKAEFDNAQSTYNQ
                     LLANIEALRATIKRRQIYAPFSGLAGIVNVNVGQYITMGTEIVRVEDQSSMKVRFTLP
                     QTNLEQISIGQKVTAVIDALPAQTFPAKIVAIDPAVDRLTGLINVEAVIEEGQEKLLS
                     GMFARLNVALPTETGQIVVPQISIAYTMYGETVYVLQPLSDEDKEMVNKMAVQNPSLD
                     VNKMYRAKQMEVKTADRKGNYSQLSKGVKAGDLIVTGGLQRLSNNALVKVSDTEAVGI
                     TTPAKNSKL"
     gene            1997..5164
                     /locus_tag="PXD71_001755"
     CDS             1997..5164
                     /locus_tag="PXD71_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL6291854.1"
                     /translation="MKFTDIFIKRPVLAVCISLLITILGLQAIEKLQVREYPEMTVSI
                     VTVSVNYSGADARLMQTLVTSQLEEAVAQADNIDYMTSSSSPSTTTVTAKMKLNTDPN
                     AALSDISAKVNAVRSNLPSGIDDPSIRVSTGSNDALMYIRFVSDELNTSQVTDYINRV
                     VKPQFFTVNGVSSVDVYGSVFGLRIWLDPDKMASNNLSGAAVLSALSANNLQTAAGNS
                     NGYYTVYRNKVLSTTPSVTELEEVTVSTTKDGKTIKLKDIATVELDKYQDAARATVGG
                     KDAVVLAVSVAATANSLTVAKDIYPMFEQVLKNLPSTIQGDIVYDKTIAIDSSIKEVI
                     KTIGEATVIVLVVIILFLGSLRAMIVPVITIPISLIGVLFFLQMFGFSINLLTLLALV
                     LAIGLVVDDAIVVLENVERHVKEGKTPFDAAIIGTREIALPVISMTITLGAVYSPMAL
                     MTGVTGTLFKEFALTLAGAVFISGIAALTLSPMMSSRILKEHKEGEESAFARVVHRSL
                     DKMTSCYTNMLSGIMAVRGFVLFFAVLIFASLPMLFGSLSSEVAPTEDRGFVVGISNG
                     PSNTNLDYTEAALAQFNQEVTKLPEVSSLMTVSGFNGANSALSIIALKDWNDRSRERA
                     AISNDVAAIGKKVVGMDVNAIAFPEISTGENDLPFSLVITTADSYEKLAIVASEFLKK
                     AQTSGQFFFANLDLKFDTVTMNMKFDREKMGAYGVTMQQVSGTLGAFLSGAIITRVDI
                     DSRAYPIVSQAVRNDRLNPEDLMNYYVTTATGQSIPLGSFVTMELKTSPSSLPRMSQL
                     NSATIGGVVSGSIGDAVNWAKAELDRTLPSGYQYDFRGESRQFVQEGNAMAMTFALAV
                     IIIYLVLAIQFESWRDPLVILVSVPLAVSGALLVMNIPTAMKGLFMGIAAGTGSKDMM
                     AFANGIWTAGYTLNIYSQVGLITLVGLITKHGILMCEVAKEEQLLHGKSRYEAIFHAA
                     TIRLRPIMMTTAAMIAGLIPLLFAVGAGAIARFSMGMVIVAGLAIGTLFTLFILPVIY
                     TFLGQEHKPLREFDEEKYFPTTKEA"
     gene            5392..6855
                     /gene="guaB"
                     /locus_tag="PXD71_001756"
     CDS             5392..6855
                     /gene="guaB"
                     /locus_tag="PXD71_001756"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL6291855.1"
                     /translation="MLRIKQEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTETKLAISLAQEGGIGFIHKNMSIERQADRVRKVKKFESGIVSEPVTISP
                     DMTLAELAELVKKNGFAGYPVIDENQNLVGIITGRDTRFVTDLSKTVREFMTPKDRLV
                     TVKENASREEIFHLMHEHRVEKVLVVNNEFQLKGMITLKDYQKAESKPNACKDEFGRL
                     RVGAAVGAGPGNEERIDALVKAGVDVLLIDSSHGHSEGVLQRVRETRAKYPDLPIVAG
                     NIATAEGAIALADAGASAVKVGIGPGSICTTRIVTGVGVPQITAIAEAAAALKERGIP
                     VIADGGIRYSGDISKAIAAGASCVMVGSMFAGTEEAPGEIELYQGRAFKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRIAYKGYLKEIIHQQMGGLRSCMGLTGCATI
                     EELRTKAEFVRISGAGIKESHVHDVTITKEAPNYRMG"
     gene            6976..7647
                     /locus_tag="PXD71_001757"
     CDS             6976..7647
                     /locus_tag="PXD71_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL6291856.1"
                     /translation="MLQAIIFDMDGVIVDTEYLEFSLQKQFIEDIKEHSRPITLEQQS
                     EVVGKCLKEIPVIIKKLSESSLPIEEIRARYYAFFQDLFSKVDFKTIFRADIQQIIQF
                     AKQNQIKLAVASSSALSHIENILTVCGIKDEFDLIVSGEQFEHSKPDPTIYRYTCEKL
                     GVEPQNAVAIEDSYYGMLAAKTAGLTVIGYEEKRILIDQSLADYMGKDMKEILKILQK
                     CNTPS"
BASE COUNT         2301 a         1438 c         1754 g         2237 t
ORIGIN      
        1 gttaagcgtt attgtttgag tgatttttgc aaagttttag ccaaaaatga ccgcttgttc
       61 aataaatgag gaattttgca actattttat tttaatttga taggtgtttt ctgctttctt
      121 ttttcataaa aaaagctata atgaacggct gttcattata ggtaaggaga taaaatgtct
      181 aggcgaagcg agccaaaaga agagatggta aaccgtattt tatcggcaac cgagtcgctg
      241 attgtgcaag aaggtttgca aaacctttca atgcgtagta ttgcaaaaga agcagggatc
      301 gcttccggta cattatatct ttactttaaa accaaagatg atttattgca ctcattggct
      361 gcccaattac tcgaatgtta taacggttat atgcattttg agtttaagcc tgaaatggcg
      421 ttatttgagc aatataaagc gttggtcaat aaaaaatggc agtttttaat cgagcgttta
      481 gatttggtaa aacagtttca agcggtgata gggattgatg aattgattcg ggaaaccata
      541 gctgatgaaa cttccttttg gaataaatta attttagaag ggaaaaagca aaggattatt
      601 gctgatctat ctaatgagtt gctttatgga ttaagcatcg gcacattggt ggatatccaa
      661 tatctgcaaa atctgaatca agaaaatcag tttgatggat atttagacga gattatttta
      721 cgcacttgga aagcaatcac tttttaagtt tctttttatt agttttatgg agaatgtatg
      781 acaacggaaa cacagaagct gggtaaaggc agaaagtttt taattgtact cacattgtta
      841 atcgtattga ttgtgtttgc tgcaatcgta gggttacaaa aaatcggggc aatgaaaaaa
      901 gccgaagcag cggcaaatat gccggaggcg gtgagcgaag ttactgctat gcaagtgacg
      961 acccaagaat ggacaccaac catttctgcg gtcggttata ttcgcccaaa tcaaggggca
     1021 atgttaagtg ctgaaacttc aggcgtggtt agccgtgttt tggtcacttc aggacagcgt
     1081 gtgaaaaaag gtgatctgtt agtagaattt gacagcagtg ttgaaattgc taacttgaga
     1141 gcctctgaag cgcaattacc gaatgccaaa gcaaatttag agcgttaccg taatctgatt
     1201 gcctcaaaca gtgcttctaa agccgagttt gataatgctc aatctaccta caaccaatta
     1261 ttagccaata tcgaggcatt gcgtgctacg attaaacgcc gtcaaattta tgctccgttt
     1321 agtggtttag cgggtatcgt gaatgtgaat gttgggcaat acattacaat gggtacggaa
     1381 attgtgcgtg tggaagatca atcttcaatg aaagtacgtt ttaccttgcc gcaaactaat
     1441 ttagagcaaa tctcgattgg gcaaaaagtg acagccgtaa ttgatgcgtt accggcacaa
     1501 accttcccag ctaaaattgt ggctattgat ccggctgtgg atcgtttaac cggcttaatt
     1561 aatgtggaag cggtgattga agaagggcag gaaaaattgc tttcaggtat gtttgcccgc
     1621 ttaaatgttg ctttaccaac agaaaccgga caaatcgtcg taccacaaat ttctatcgct
     1681 tacactatgt atggtgaaac ggtatatgtg ctgcaaccat tatctgatga agataaagaa
     1741 atggtgaaca aaatggcggt acaaaaccca agtttagatg taaacaaaat gtaccgtgca
     1801 aaacagatgg aagtgaaaac ggctgaccgc aaaggaaact attcacaatt atccaaaggc
     1861 gtgaaagcag gtgatctgat tgtaacaggc ggtttacaac gtttaagtaa taatgcgttg
     1921 gttaaagttt ctgatacaga agccgtaggc attaccacac cggcgaaaaa cagtaaactg
     1981 tagtggagtg agttaagtga aatttactga catctttatt aaacgtcctg ttttggcagt
     2041 ttgtatcagc ctattgatta cgatcctagg cttgcaagca atcgaaaaat tgcaggtgcg
     2101 tgaatatccg gagatgacgg tctcgattgt aaccgtgagt gttaactatt caggtgcaga
     2161 tgcacgttta atgcagacac tggttacctc acagttagaa gaggctgttg ctcaagcgga
     2221 taatattgac tatatgactt catcaagctc tccaagtacc actacggtaa cggcaaaaat
     2281 gaagttaaat accgatccga atgccgcact ttccgatatt tcggcaaaag tgaatgcggt
     2341 acgctcaaat ttaccgagcg gtattgatga tccgtcaatt agagtctcaa ccggttcaaa
     2401 tgatgcgtta atgtatatcc gatttgtctc tgatgaactt aatacatcac aagttaccga
     2461 ttatattaac cgtgtagtaa aaccacagtt ctttacggta aatggtgttt ctagcgttga
     2521 tgtgtatggc tctgtttttg gcttacgcat ctggcttgat ccggataaaa tggcgagcaa
     2581 taacctatca ggggcggctg tattatcggc attaagtgcc aataacttgc aaaccgctgc
     2641 gggtaattct aacggttact atacggttta tagaaacaaa gtactttcta ccacgccttc
     2701 tgtgacagag ttagaagagg ttacggtttc aaccaccaaa gatggaaaaa ccatcaaatt
     2761 aaaagatatt gcgactgttg agctggataa ataccaagat gcagcaagag caacggttgg
     2821 cggtaaagat gcagttgtgc ttgctgtttc ggtagccgca acggcaaact ctttaaccgt
     2881 agcgaaagat atttatccga tgtttgagca ggtattaaaa aatctaccaa gcaccattca
     2941 aggagatatt gtttacgata aaaccattgc gattgatagt tctatcaaag aagtaattaa
     3001 aactattggt gaggcaaccg tcattgtatt ggtagtaatt atcctgttct taggttcatt
     3061 acgagcaatg attgtaccgg tgattaccat tccgatttcc ttaatcgggg tattattctt
     3121 cctgcaaatg tttggtttct cgatcaactt gcttactttg ctggcgttgg tattagcgat
     3181 cggcttggta gtggacgatg cgattgtcgt acttgaaaac gtagaacgtc acgttaaaga
     3241 aggtaaaacg ccatttgatg cagcgattat cggtacacgt gaaattgctt taccggttat
     3301 ttcaatgact attactctgg gtgcagtata ttcaccaatg gcattaatga ccggggtaac
     3361 agggacacta tttaaagagt tcgctttaac gcttgccggt gcggtattta tttcggggat
     3421 tgcggcatta accctttcgc cgatgatgtc tagccgaata ttaaaagaac ataaagaagg
     3481 ggaagagtca gcatttgccc gcgtggtgca tagatcacta gataaaatga ccagctgcta
     3541 taccaatatg ctaagcggta ttatggctgt tcgtggtttc gtcttattct ttgcggtgct
     3601 gatcttcgcc agtttaccga tgttatttgg ctcactttcc agtgaagttg caccgacaga
     3661 agaccgtggc tttgtggttg gtatttctaa tggaccttca aacaccaatt tagattatac
     3721 cgaagcagcg ttagcccaat ttaatcagga agtaactaaa ttacctgaag tatcttcctt
     3781 aatgacagtt tcaggcttta atggtgcaaa tagtgcatta tcgattattg cattaaaaga
     3841 ttggaatgat cgtagccgtg aacgtgctgc gatttctaat gatgtggctg caatcggtaa
     3901 aaaagtggtg ggtatggacg tgaatgcaat tgcattccct gaaatttcaa ccggtgaaaa
     3961 tgatctacca ttctcattag tgattactac cgctgatagc tatgaaaaat tggcaatcgt
     4021 tgcctctgaa ttcttgaaaa aagcccaaac ttccggtcag ttcttctttg cgaatttaga
     4081 tttgaaattt gatacggtca cgatgaatat gaaattcgac cgtgaaaaaa tgggtgctta
     4141 tggcgtgaca atgcagcagg tgagtggcac attaggggca ttcttatccg gtgcgattat
     4201 tactcgtgtc gatattgact ctcgtgctta cccgattgtg tcgcaagcgg tgcgtaatga
     4261 tcgtttaaat cctgaagatt taatgaatta ctatgtcacg actgcaacag ggcaatctat
     4321 cccgttaggt tcatttgtga cgatggaatt aaaaacatca ccatcttctt tacctcgtat
     4381 gagccaatta aactcggcca ccattggtgg ggtagtatct ggctcaatcg gcgatgcggt
     4441 aaattgggcg aaagcagaat tagatcgcac tttaccatca ggttatcaat atgacttccg
     4501 tggtgaatct cgccagttcg ttcaagaagg taatgcgatg gcaatgactt tcgctctagc
     4561 agttatcatt atctatcttg tacttgcaat tcaatttgaa tcttggcgtg acccattagt
     4621 aattttggtt tctgtgccgt tagctgtgag tggtgcattg ttggtaatga acattccaac
     4681 cgcaatgaaa ggcttgttta tggggattgc agcaggcact ggctcgaaag atatgatggc
     4741 attcgccaac ggtatttgga cggcaggtta taccttaaat atctattcac aagtaggtct
     4801 aattaccctt gtcggcttaa ttactaaaca cggtatcttg atgtgtgaag tggcgaaaga
     4861 agagcagtta ctgcacggca aatcacgcta tgaagctatt ttccacgcag ccacgattcg
     4921 tttacgccca attatgatga caacggcagc aatgattgca ggcttaattc cgcttctgtt
     4981 tgcggtgggt gccggtgcaa tcgcccgctt cagtatgggt atggtaatcg tagcaggctt
     5041 ggcaatcggg acattgttta ctctgtttat cttgccggtt atctacactt tcttagggca
     5101 agaacacaag ccattacgtg aatttgacga ggaaaaatat ttcccaacga caaaagaagc
     5161 atagttttaa atagcaaaag agcgtagcga tacgctcttt tttgttggct tacaagcggt
     5221 cgttttttat ttattgtttg ctaaaaaatc acaaaattta accgcttgaa agtatatttt
     5281 cagatagcgt aaacgtttcc ctatttacag aaacctagtt cctctgtata atccgctcgc
     5341 aattttccag tacagatttt caatttaatc attttttaag gaataattgc aatgctacga
     5401 attaaacaag aagccctcac ttttgatgat gttcttctcg tcccggcaca ttctactgtg
     5461 cttcctaata ctgctgatct ttctactcaa ttaactaaaa ccattcgttt aaacattccg
     5521 atgctttctg ctgcaatgga taccgttaca gaaactaagc ttgcgatctc ccttgctcaa
     5581 gaaggcggca ttggttttat ccataaaaat atgtcgattg aacgccaggc agaccgtgtg
     5641 cgtaaagtga aaaaatttga aagtggtatt gtttctgagc cagtgacgat ttctcctgat
     5701 atgacattag cggaattggc tgaattggtg aaaaagaacg gttttgcagg ctatccggtg
     5761 attgatgaaa accaaaattt agtgggaatt attaccggac gtgatacccg atttgtcacg
     5821 gatttaagca aaacagtgcg tgaatttatg acaccaaaag accgtttagt gacggtaaaa
     5881 gaaaacgcaa gccgtgaaga aattttccac ttaatgcacg aacaccgagt ggagaaagtg
     5941 ctggtagtga ataatgaatt tcagttaaaa ggaatgatta ccctaaaaga ctaccaaaaa
     6001 gcggaaagca aaccgaatgc ctgtaaagat gagtttgggc gtttgcgtgt gggggcggca
     6061 gtgggagccg gtccgggcaa tgaagaacga attgatgctt tagtaaaagc gggtgtcgat
     6121 gtgctattaa tcgactcttc gcacgggcat tctgaaggtg tattacaacg tgtgcgtgaa
     6181 acccgtgcaa aataccctga tttaccgatt gttgccggta atattgccac tgcagaagga
     6241 gcgattgcgt tagctgatgc aggagccagt gctgtgaaag taggaatcgg cccgggttca
     6301 atttgtacca ccagaattgt aacaggcgtt ggcgtgccac aaatcacggc aatcgcagaa
     6361 gcggcagctg cgcttaaaga acgaggcatt cctgtgattg ctgatggtgg aattcgttat
     6421 tcaggcgata tttcaaaagc tattgccgcc ggtgcaagtt gcgtaatggt cggttcgatg
     6481 tttgccggca cagaagaagc cccgggtgaa attgagcttt atcaaggcag agcattcaaa
     6541 tcctaccgtg gaatgggatc attaggtgca atgagtaaag gctcgtcaga tcgctatttc
     6601 caatctgata atgccgccga caagctcgta ccggaaggga ttgaagggcg tatcgcttac
     6661 aaaggctact tgaaagaaat tatccaccaa caaatgggcg gcttacgctc ctgtatggga
     6721 ttaaccggct gtgccactat tgaagaactc cgcaccaaag cagaatttgt ccgcattagt
     6781 ggtgctggta ttaaagaaag ccacgtccac gatgtgacaa ttaccaaaga agcaccgaac
     6841 taccgaatgg gttaatagcc tttctcactc tctccccttg tgggagagag accgttttaa
     6901 attacgttca gtaatttaaa acagagagag ggaataagtg gttagatttt cttaaaattt
     6961 tacaaaagag gctatatgct ccaagctatc atttttgata tggacggcgt gattgtggat
     7021 accgaatatc tggaattttc cttacaaaaa cagtttattg aagacatcaa agaacacagc
     7081 cgcccgatta ctttagaaca acaatcggaa gtggtaggca aatgtttaaa agaaattccg
     7141 gtgattatca aaaaactaag cgaatccagt ttgccgattg aggaaattcg tgcacgttac
     7201 tacgcctttt tccaagatct gtttagcaaa gtggatttta aaaccatttt cagagccgat
     7261 attcagcaaa ttattcaatt tgccaagcag aatcaaatta agcttgcggt tgcctcttct
     7321 tccgcccttt cgcatattga aaatattctc acggtttgcg gcattaaaga cgaatttgat
     7381 ttaatcgtca gcggtgaaca gtttgaacac agcaagccag acccaaccat ttaccgctac
     7441 acctgcgaaa aattaggcgt tgagccacaa aatgcagtgg cgattgaaga ctcctactac
     7501 ggaatgctcg ccgccaaaac cgcaggctta acggtgattg gttacgaaga aaaacgaata
     7561 ctgattgacc aaagtttagc ggattatatg ggcaaagata tgaaagagat tttgaagatt
     7621 cttcaaaaat gtaatactcc aagttaataa acggtcgatt tttatagaaa ttttgtcaat
     7681 gtttctactt caagtacttg gaacagtcta tcggcttgag taacctagaa
//
LOCUS       DANLXS010000107         7603 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.107, whole genome shotgun sequence.
ACCESSION   DANLXS010000107 DANLXS010000000
VERSION     DANLXS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.107"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            139..612
                     /locus_tag="PXD71_001758"
     CDS             139..612
                     /locus_tag="PXD71_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL6291857.1"
                     /translation="MAIAKAKTESFKDSAKNKMDAVKATTAEKTDVIKAKAASASDTM
                     KVKAQEAKAATAEKAATAHETLNSAKTATAEKAASMKAAATEKMSAVKKSQKVNINTA
                     NEEQLQSLTGIGEAKAKAIVEYRKKHGKIKNAAELANIPGIGDATIEKVAPYLTF"
     gene            760..1389
                     /locus_tag="PXD71_001759"
     CDS             760..1389
                     /locus_tag="PXD71_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6291858.1"
                     /translation="MALDFNSVGLDSVEAKGGYGIGLQIGQQLLGSGMDVNAETVARG
                     IYDVLNNNEPAIDINEVTAALQELGQRAEAAQAEAFKAIDAENKAFLEENKKANGVIV
                     TDSGLQYEILTEGTGEKPTATSTVRVHYTGSLIDGTVFDSSVKRGQPAEFPVNGVIRG
                     WTEALQLMPVGSKWRLTIPQELAYGERGAEASIPPFATLVFEVELLDIL"
     gene            1455..1970
                     /locus_tag="PXD71_001760"
     CDS             1455..1970
                     /locus_tag="PXD71_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDL6291859.1"
                     /translation="MQKKSLISRLLKNIFLYGSLFIIISLVVDWYRSPSAPAQFEQTV
                     HYDIQHQPKVIAQLSHEKPMLLYFWGSWCHYCQVVSPNIQQLANDGTEVLGVALKSGD
                     ENDVQAYLNENGYTFATLNDPSGDFSRGWNIQATPTILIIKDGKIAHHTTGYTSYLSL
                     KFRLWLADVLY"
     gene            complement(2018..2948)
                     /locus_tag="PXD71_001761"
                     /pseudo
     CDS             complement(2018..2948)
                     /locus_tag="PXD71_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3007..3657)
                     /locus_tag="PXD71_001762"
     CDS             complement(3007..3657)
                     /locus_tag="PXD71_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha4 family transposase"
                     /protein_id="HDL6291860.1"
                     /translation="MRKSRLSQYKQNKLIEQFVAGVTARTASELVNVNKNTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEVDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQFATLLPIIREKVKPDSIVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFGSQAKRHLRKFNGIPKVHFELYLKECEWRFNHSDLKSQISFLKQLVKGSLG"
     gene            3986..5344
                     /gene="mnmE"
                     /locus_tag="PXD71_001763"
     CDS             3986..5344
                     /gene="mnmE"
                     /locus_tag="PXD71_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL6291861.1"
                     /translation="MKDTIVAQATPIGRGGVGILRVSGPLAETVAQAVLGKTLPPRIA
                     NYLPFKDEDGTVLDQGIALFFKAPNSFTGEDVLELQGHGGQVILDLLLKRILQVKGLR
                     IARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINALVDS
                     VIYLRTYVEAAIDFPDEEIDFLADGKIEAKLNEIIAQLDGVRREAKQGSILREGMKVV
                     IAGRPNAGKSSLLNALAGREAAIVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAS
                     DEVERIGIQRAWEEIAQADHVLLMIDSTEQQADQFRQEWAEFLAKLPANMPVTVIRNK
                     VDLSGEQEGLIQVDDFTMIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQ
                     ALETAAEHLERGHIQLTQFLAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCI
                     GK"
     gene            5345..6610
                     /gene="tilS"
                     /locus_tag="PXD71_001764"
     CDS             5345..6610
                     /gene="tilS"
                     /locus_tag="PXD71_001764"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL6291862.1"
                     /translation="MALFNRFQTQCATHLPNQTAFLIGLSGGVDSVVLLHLFARTSFH
                     VRAIYVHHGLSPNADDWAAFCEQYCKRLNIPFILQKVRVDSSNGVENGAREARYQAIQ
                     QQLKPNEVFATAHHLDDQAETFLLALKRGSGVKGLSAMQAVTFLQNFTVFRPLLTFTK
                     SDLMGYAVQHQLGWIEDESNADNRYDRNFLRNSILPLLNQRWQHFSQMVARSAQHCAE
                     QQALIEELLSNELKSRTGEKQQLNINGFGQFSLAKQQQLSRLWLEQNGVRMPSQAQLQ
                     AVISELIFAKADKNPQVKVGENIIRRYQQAIYVTEEIADIPPFEVVLPTETKVELPYQ
                     LGTIIRQQQEIICKKNGKNHRLQLPQELAQSTISIKIGQLGKVKCYGKAQREEMKKIW
                     QNHGIPVWERNRTPLIFWQDELVGLLPIL"
     gene            complement(6600..7493)
                     /gene="xerD"
                     /locus_tag="PXD71_001765"
     CDS             complement(6600..7493)
                     /gene="xerD"
                     /locus_tag="PXD71_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL6291863.1"
                     /translation="MAELDPIIEQFLDTLWQEHGLSENTVASYRSDLERFSDYFSEPK
                     AFLSLDHIDLQGFLGERLEQGYKATSSARMLSCLRKFFRFLCLEHYRQDDPTLTLSSP
                     KRGASLPKSLSEDQVMDLLDAPNTLDPIELRDKAMLELLYATGLRVTELVSLSMDNIS
                     LKQGVVRIIGKGDKERLVPLGEEANYWIQEFFQYGRPILLNNQQSDVVFPSRRGQQMT
                     RQTFWHRIKHYAVLAGIDADKLSPHVLRHAFATHLVNHGADLRVVQMLLGHSDLSTTQ
                     IYTQVAKARLKSLHQQFHPRG"
BASE COUNT         2355 a         1734 c         1625 g         1889 t
ORIGIN      
        1 tcatttgcac aaaccactca acctactgcg gctgaacaaa tgaacgcagt taaagcctct
       61 gttgccgaaa aagcaactgt ggctaaagaa gcaacaaaag agaaagcagc ggagttaaaa
      121 gccggttctg cggaaaaaat ggcgatagca aaagccaaaa ccgaatcatt taaagattcc
      181 gctaaaaaca aaatggacgc agtaaaagcc accacagcag aaaaaacgga cgtgattaaa
      241 gcgaaagccg catctgcaag cgacacaatg aaagtgaaag ctcaggaagc aaaagccgcc
      301 accgcagaga aagctgcaac tgctcatgaa accttaaatt cagccaaaac cgcaaccgct
      361 gaaaaagcgg cctcaatgaa agcagctgca acagaaaaaa tgtcagcagt gaaaaaatca
      421 caaaaagtga atatcaacac tgccaacgaa gagcaattac aatcgcttac cggcattggt
      481 gaagcaaaag ccaaagcgat tgtagaatac cgcaaaaaac acggtaaaat caaaaatgca
      541 gcagaactgg cgaacattcc cggcattggc gatgcaacca ttgagaaagt tgctccatat
      601 ttaactttct aatgttctgc aagcggttat tttgttgcaa aattttgcag aaaaataacc
      661 gcttgttcat ttcacacctt gtattcttaa atttcacccc cattaagtga atcgtatttc
      721 tttcaataca gtattcaata aactcaaaag gaacagaaaa tggcattaga tttcaattca
      781 gttggattag attcagtaga agcaaaaggc ggttacggca tcggtttaca aattggtcag
      841 caacttttag gcagcggaat ggacgtaaac gcagaaaccg ttgcccgtgg tatttatgat
      901 gtgttaaaca acaatgagcc ggcaatcgac atcaatgaag tgaccgctgc attacaagaa
      961 ttaggtcagc gtgcggaagc ggctcaagca gaagcattca aagcgattga tgccgaaaac
     1021 aaagcgttct tggaagagaa taaaaaagca aacggtgtaa tcgttaccga cagcggttta
     1081 caatatgaaa ttttaaccga aggcacaggc gaaaaaccga ctgcgacttc aaccgtgcgt
     1141 gttcactaca ccggttcatt aattgacggc accgtgtttg acagctcggt aaaacgcggt
     1201 cagccggcag aattcccggt aaacggagta attcgtggtt ggacagaagc attgcaatta
     1261 atgccggttg gctcaaaatg gcgtttaacc attccacaag aattagccta cggcgaacgt
     1321 ggtgcagaag catcaattcc accatttgcg accttagtgt ttgaagtgga attgcttgat
     1381 attctctaat caaactcaaa taaaaaacct gtggaggcat ttcacaggtt tttgctattt
     1441 aagataacta tattatgcag aaaaaatcac tcatatctcg cttacttaaa aatattttcc
     1501 tctacggcag cctgtttatc attattagcc tggtggttga ttggtatcgc tcgccctccg
     1561 ctccggcaca atttgagcaa accgtgcatt atgatattca acaccagccg aaagtaatcg
     1621 cccaattaag ccacgagaag ccgatgctac tctatttttg gggaagttgg tgccactatt
     1681 gccaagtggt ttcgccaaat atccaacaat tagcgaatga cggcacggaa gttttaggtg
     1741 ttgccctgaa atccggcgat gaaaatgacg tacaagccta tctcaatgaa aatggctaca
     1801 ctttcgctac tttgaatgac ccaagcggtg atttctctcg aggttggaac attcaagcca
     1861 ccccaaccat cttgattatc aaagacggta aaatcgccca ccacaccacc ggctacacta
     1921 gctatttgag cttaaaattc agactatggc tggcagacgt gttgtattaa acgattcctt
     1981 tcatttgcaa aaaaatccac taaatccgac cgcttgttta caccaaaaag acaagcggta
     2041 ctaaattcat tgtcatcatt gtggtgatcg ataaaatcag cctcgatgag ccaaaaataa
     2101 agcctcggtt tttgccgttg tcgaatttca ccagtaaatt tgccattgct gtgagggcgt
     2161 acacttccac caccgtaaaa atcacggtgt aaatataggc agcggtcaga gttgcgactt
     2221 ttaccgcaat aaaccaaggg gcaataatcg ccaccgctaa taacggcgaa atccaaatta
     2281 attgctgttt ggtgaggttg attttacgag aaaaagtcga ttgcaaactg acggttaaaa
     2341 caccgttgat gaataacgca atcggcgaat gctccggtgc gtgcaggaca ttgtcgaata
     2401 aatacggaaa aatcactcag cggcacaaat agcattaagt gagcgaacaa aaactctttg
     2461 ttgatgattt ccttaatttg tgcgaaacga aatttcacgc cgccattcac gccttccacc
     2521 gaataaaacg gcttgaccat aaacgcaaac aagccggctt ccaaaataaa gcagatccaa
     2581 atcagcgttt caatcatctc aaacctaata aacggaataa taatcagcgg ggcgagcatt
     2641 gaagacatac tggtgacttt tagatatttg ccttgtaaac gggttttagc agcatattca
     2701 tcgccggcaa tcgccagtaa acaggctctg gcattagtgc cgaataagca actccccaac
     2761 ccaaagcaag tggttgccaa caataaaatg aggtaattat ccgctgctaa aaacagcata
     2821 taggcaacca catctaataa acagcccaat aacatcattt ttgccaagcc gtagcgatcc
     2881 ccccaaatgc cggctaaaat tgccaatgcc tgattacaaa acaacagcaa tgaaaggaaa
     2941 aaagcgattt gggcgttatc tagccctttc tcttttaaat aaaagaaaag gggctgtcct
     3001 agataactaa cctaaactac ccttaaccaa ttgttttaaa aaggatattt gagattttaa
     3061 gtcactgtga ttaaaacgcc attcgcattc tttcaaatac agctcaaaat gcactttggg
     3121 aatgccgtta aacttacgca aatggcgttt tgcttggctc ccaaaattct caattccatt
     3181 aatgtgattt tgattttcag cgaagtgtgt gctgtgatta atgcgaaaat ggttaaattc
     3241 actcacatca agcacatcat aacttcgata gaaatcggtg taaacaatac tatcgggttt
     3301 caccttttcc cgaataatcg gcaaaagtgt ggcaaattgc gtgtttggta ctaccacggt
     3361 ataaaccttt ccgtcccgct ttaaaagccc gaacacggcg gttttgcctg ctgcaccacg
     3421 tccacgttta cctttacgat gaccgccgaa ataactctca tcaacctcaa tttctccctc
     3481 aaacatttcc aaatgagggc tatgttgata gataagcaac cgcaagcgat ggaagtaata
     3541 cgccgccgtg tttttgttta cattgaccaa ctcagatgct gttcgggctg ttacccctgc
     3601 aacaaactgc tcaatgagtt tgttttgttt gtactgactt agacgacttt ttctcatttg
     3661 ggtatcttaa cctaaatgga gtttttagtc gttatctagg acagccccaa agaaaaacac
     3721 gctttgcatt gaaaaaaagg cggtggtgga aataaaacgg cgtaggagta atagtttttc
     3781 taaagacatt cagaatggtt gaattggtta aataaagagc ggttattcta ccttttaagt
     3841 caaccccaat caatcggctt ttgttttacc tttggcgtgg gtttcggtat gataagcgat
     3901 atgtagtggc gggtcctgtg cccgcctaaa taccaatgat aacttgcggg cgagcacagg
     3961 actcgcccct acgataaaca agaaaatgaa agacaccatt gttgctcaag caacaccgat
     4021 tggcagaggc ggtgtgggga ttctgcgagt ttccggtccg cttgccgaaa ccgttgctca
     4081 agccgtatta ggcaaaacat tgccaccccg tatcgcaaat tatttgccgt ttaaagacga
     4141 agatggcact gtgttggatc aaggcatcgc cttatttttt aaagctccca actcctttac
     4201 cggcgaagat gtgcttgaac ttcaaggaca cggtgggcag gttattttag atttactgct
     4261 caaacgcatt ttacaagtta aggggctacg cattgcccga gcaggtgaat tttccgaaca
     4321 ggcattttta aacgataagc tcgaccttgc ccaagctgaa gcgattgcgg atttaatcga
     4381 cgccaccagc gagcaagccg ctcgttcagc cctgaaatca ctacagggtg aattttctaa
     4441 taaaatcaat gcgttagttg attcagtaat ttatttacgc acttatgtgg aagcggcgat
     4501 tgacttcccc gatgaggaaa ttgactttct ggcagacggt aaaatcgagg caaaattaaa
     4561 tgagattatc gctcaattag acggtgttcg ccgtgaagcg aaacaaggct cgattctgcg
     4621 tgaaggaatg aaagtagtga tcgccggtcg cccgaatgca gggaaatcaa gcttgcttaa
     4681 cgccttagcc ggacgagagg cggcgattgt aaccaatatt gctggcacaa cccgtgatgt
     4741 gttgcgtgaa catattcaca ttgacggaat gccgttgcat attatcgaca ccgccggtct
     4801 ccgtgatgcc agtgatgagg tggaacgtat cggcattcag cgtgcttggg aagaaatcgc
     4861 ccaagccgac cacgttttat taatgattga cagcaccgag caacaagccg atcagttccg
     4921 acaagagtgg gcggaatttc tagccaaact gccggcaaat atgcctgtga ccgtaattcg
     4981 caacaaagtc gatttatccg gcgagcagga aggcttaatt caggtggacg atttcacgat
     5041 gattcgcctc tctgcccaaa ccaaagtcgg cgttgattta ttgcgtgaac acttgaagaa
     5101 atcaatgggc taccaaagtt ctacggaagg cggattcttg gctcgccgcc gtcatctgca
     5161 agcattggaa accgctgccg agcatttaga gcgagggcat atccagctta ctcaattctt
     5221 agccggcgaa ttgttagcgg aagagttgcg tatggtgcaa aatgccttaa gtgaaattac
     5281 cggccagttc acctctgatg acttattagg caacattttc agctcatttt gcatcggtaa
     5341 ataaatggca ctttttaacc gctttcaaac ccagtgtgca acgcatttgc cgaaccaaac
     5401 cgccttttta atcggcttaa gcggtggggt ggattcggtg gtactactcc acctttttgc
     5461 ccgcacctca tttcacgtta gggcaattta tgtccaccac gggctaagcc caaatgcgga
     5521 cgactgggca gccttttgcg aacaatattg caagcggtta aatattccct ttattttaca
     5581 aaaggtcaga gtggatagct caaacggcgt tgaaaacggg gcgagagaag ctcgttacca
     5641 agccattcaa caacagctta agcccaatga agtgttcgcc accgcccacc atttagatga
     5701 ccaagccgaa acttttttgc tcgccctcaa gcgaggcagt ggagtcaaag gattatcggc
     5761 gatgcaagcg gtcacttttt tgcaaaattt taccgttttt cgaccgctac tcacttttac
     5821 taagtcagat ttgatgggtt atgccgttca acatcagcta ggttggattg aggacgaaag
     5881 caatgccgat aaccgttacg accgtaattt ccttcgcaat agcatcttgc cactactcaa
     5941 tcaacgttgg cagcacttca gccaaatggt tgcccgttcc gctcagcatt gtgcagaaca
     6001 gcaagccttg atagaagagc tattaagcaa tgaactcaag tcaagaacag gggaaaaaca
     6061 acaactcaac attaacggct ttgggcaatt ttcacttgcc aagcaacagc aacttagccg
     6121 cctttggctg gaacaaaacg gagtgagaat gccaagccaa gcccagctac aagcggtcat
     6181 ttctgaactg atttttgcaa aagccgataa aaacccacag gttaaagtcg gcgaaaatat
     6241 tatcagacgc tatcaacaag ctatttacgt taccgaagag atcgccgaca ttccgccgtt
     6301 tgaggtagta cttcccacag aaaccaaagt ggaattaccc tatcagctcg gtactattat
     6361 tcgccaacaa caagagatca tttgcaaaaa aaacgggaaa aatcaccgct tgcaactgcc
     6421 ccaagagctg gctcaatcaa ctatttctat taaaatcggg caactaggca aagtaaaatg
     6481 ctacggcaag gctcaacggg aagaaatgaa aaagatttgg caaaatcacg gcatacctgt
     6541 ttgggaacgc aaccgtacac cgctgatttt ttggcaagac gaattagttg gcctattacc
     6601 tatcctctag ggtgaaattg ctgatgaagc gatttcaaac gggcttttgc cacttgagtg
     6661 taaatctggg tggtcgataa atcactatgc cccagcaaca tctgtaccac tcgcaaatca
     6721 gccccatgat tgaccaaatg ggtcgcaaag gcgtggcgaa gcacgtgagg cgagagttta
     6781 tctgcatcaa tacctgcaag cacggcatag tgcttaatcc gatgccaaaa cgtctgcctt
     6841 gtcatctgct gacctcgccg gctcggaaat acaacatcag actgctgatt attcagcaaa
     6901 atagggcgac cgtactggaa aaactcctgt atccaataat tcgcctcctc gcccaatggc
     6961 actaaccgct ctttatcccc tttaccgata attctcacca ccccttgttt gaggctgata
     7021 ttatccatcg aaagcgacac cagctccgtt acccgcagac cggtggcata cagcagctcc
     7081 agcattgctt tatcccgtaa ctctatcgga tctaacgtat tcggggcatc tagcaaatcc
     7141 attacctgat cttcacttaa ggatttgggt aaagaagcac cccgtttagg ggaagaaagg
     7201 gttaaagtcg ggtcatcttg gcgataatgc tcaaggcata aaaaacggaa aaatttcctt
     7261 aaacaactca acattctggc agagcttgtc gccttatagc cttgctccaa acgctcgccg
     7321 agaaaacctt gtaagtcgat atgatcgaga gacagaaatg ctttaggttc agaaaaataa
     7381 tcggaaaaac gctctaagtc agaacgataa gaggcaacgg tattttcaga caaaccgtgt
     7441 tcttgccata gtgtatcgag aaattgttca ataatagggt caagctcagc catacaagcg
     7501 ggtatatttt caggaaattt tgcaaatttt aacagaatta aatctataaa aatacaaaag
     7561 cgatagaaac gaaaaaaccg attagcttat gctaatcggt tta
//
LOCUS       DANLXS010000108         7546 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.108, whole genome shotgun sequence.
ACCESSION   DANLXS010000108 DANLXS010000000
VERSION     DANLXS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.108"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            53..1516
                     /gene="ubiD"
                     /locus_tag="PXD71_001766"
     CDS             53..1516
                     /gene="ubiD"
                     /locus_tag="PXD71_001766"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDL6291864.1"
                     /translation="MKYKNLREFLDFLESQGELVRIQQEIDPYLEMTEIADRTLRKGG
                     PALLFENPKGYQMSVLCNLFGTPKRVALGMGQEDTKALRELGKLLAFLKEPEPPKGFK
                     DLIGQLPQWKQILNMPPKVLSKADCQQVVISGDEVDLYKLPIMHCWPGDVAPLVTWGL
                     TITQGPYKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWKAENPGKPFLVSVAI
                     GADPATILAAVTPVPDTLSEYAFAGLLRGHKTEVVKSISNELEVPASSEIVLEGYIDL
                     EETALEGPYGDHTGYYNEQEYFPVFTVTHITMRKDAIYHSTYTGRPPDEPAVLGEALN
                     EVFIPILQKQFPEIVDFYLPPEGCSYRLAVVTMKKQYAGHAKRVMMGVWSFLRQFMYT
                     KFVIVCDDDVNARDWKDVIWAITTRCDPARDTTLIEHTPIDYLDFASPIAGLGSKMGI
                     DATNKWAGETTREWGTPIEKDDNVVKRIDEIWDSLGI"
     gene            complement(1601..2425)
                     /locus_tag="PXD71_001767"
     CDS             complement(1601..2425)
                     /locus_tag="PXD71_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6291865.1"
                     /translation="MDSLTHLFTQFQFRTDLFHLGQLCQIGSFNEENKGYLHFVRQGR
                     FILNQAAERPITIERPAIIFSPTNILHSLHPLDDSGLDIFCINFDFGKGIRNPLTHTL
                     HNIVILELAQYPELGVLADQIFSEVNQKGCGYQAMIHHLCAYFTLKVARQCLEKGYIQ
                     TGLLKGLADKQLGKVLLAIHNAPERDWNVEELANVALMSRSRFAAYFKQTMGVSPMDY
                     LTNWRLAVAQHLLQKGIPVALVAEQVGYSHNAALSRVFMRELGLSPTEWLAQNKES"
     gene            2552..3397
                     /locus_tag="PXD71_001768"
     CDS             2552..3397
                     /locus_tag="PXD71_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL6291866.1"
                     /translation="MMSQEQFEEINQERRQALNHGSRIAMAASVLGALGIASTANAKQ
                     ASAEKSPYAEPEQYGLKRHGMTIDPKRVALVVVDPQIDFLGPKGVMWGVLGDSIKENN
                     TVANIESLFKAAKAVDMPTFVSPHYYYPTDHKWEFGSPGEHFMHDSGMFARKGQYTME
                     GFENSGADFMPEYKPYIFDGKTVITSPHKIFGPETNDLVLQLRKRKIDQVILAGMAAN
                     LCIDSHLRELIEQGFEVTVVKDATAGPRIPEGDGYLAALTNYRIIANDLWTTEEAVKK
                     MTAKA"
     gene            3854..6796
                     /gene="rne"
                     /locus_tag="PXD71_001769"
     CDS             3854..6796
                     /gene="rne"
                     /locus_tag="PXD71_001769"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDL6291867.1"
                     /translation="MKRMLINATQKEELRVALVDGQRLFDLDIESPGHEQKKSNIYKG
                     KITRVEPSLEAAFVDYGAERHGFLPLKEISREYFPSDYVFNGRPNIKDIIQEGQEVIV
                     QVSKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERLELKEALDCLDV
                     PEDVGLIVRTAGVGKSPEELQWDLKVLLHHWEAIKKAAESRPAPFLIHQESDVIVRAI
                     RDYLRRDIGEILIDNKKVFEKAKNHIRLVRPDFINRVKLYEGEVPLFSHYQIESQIES
                     AFQREVRLPSGGSIVIDVTEALTAIDINSSRSTRGGDIEETALNTNLEAADEIARQLR
                     LRDLGGLIVIDFIDMTPVRHQREVENRIREATRQDRARIQFARISRFGLLEMSRQRLS
                     PSLSEASSHICPRCQGTGKVRDNESIALSILRLLEEEAIKENTAQVHTIVPVQIASYL
                     LNEKRKAISSIEKRHDVQIVVVPSESMETPHFSVYRLRENEITPTLSYDLTKHYEARH
                     EEEHPHHHHQEDVLVTRNEPVITVESVLQSSELNLQPSPTPTEPPKPSLLSRLIATIK
                     GWFSEEKVEEKPKPKARNQRERRQRRERPNRNAKAENQERKTPQAQTTPAEEKPMRSE
                     KKARRAVVEEVSGVTEQESRSNSREVQTERRQRRDLRKKVRIETQEVEVENLTPAVEE
                     TVAKQVENVQKNQQIQPLATQEVPVETAPEVETTNEGRENNRQRRLPRHLRVGNQRRR
                     ENQRNQVISPMPLTSAVASPELASGKVWVQVTSTVKVEEKASFLSVDQMLEQQENQLV
                     ETEKKSEEKKLHTVSPLGGFVAQATNRDLVKEKQEQLSKPKEEKVASEDVKPVEVKEH
                     IAPFGSVVAQHSERDLVKEKNERVSKFQSQATVAPAIVEEVTNPIEVPKSTYESDYRF
                     NGRLGTFSRVEHTQAAMTIASAPSDKLEVVEISQWQNSKYYFYGKGFGGYTSAVSQVQ
                     SAPRSTNG"
     gene            6942..7502
                     /locus_tag="PXD71_001770"
     CDS             6942..7502
                     /locus_tag="PXD71_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291868.1"
                     /translation="MKKSFLSLLVLGTTMALAACDQAKEAAQSTTDKAVEVKEAVAEK
                     AAEVKSATEAKVEELKAAANDAAQATADKTEEAKKAVAEKATELKTTTEAKVEELKAA
                     ANDAAQATTDKTEEAKKAVAEKAAELKATTEAKVEELKAAASDATQATADKAEEAKKA
                     VAEKAAEVKVETETKVEEMKKAIESK"
BASE COUNT         2443 a         1593 c         1651 g         1859 t
ORIGIN      
        1 ttcatttacg tttttctata tacttgcaaa aaaatcgaaa aatatgaccg ctatgaagta
       61 taaaaatctc cgtgaatttc tcgatttcct tgagtctcag ggtgaattag tccgtattca
      121 gcaagaaatc gatccttatt tagaaatgac agaaatcgcc gaccgcacct tacgcaaagg
      181 cgggccggca ttactgtttg aaaacccgaa aggctatcaa atgtcggtgt tgtgtaattt
      241 attcggcacg cccaaacggg tagctctggg aatggggcaa gaagatacca aagctctgcg
      301 tgagctgggc aagttacttg cctttttaaa agagcctgaa ccaccgaaag ggtttaaaga
      361 tttgatcggg caactgccac aatggaagca aattttaaat atgccgccta aggtactgag
      421 caaagccgat tgtcagcaag tggtgatttc gggtgatgag gttgatctct acaaattacc
      481 gattatgcac tgttggccgg gcgatgtcgc tccactggtc acttgggggc tgacgattac
      541 gcaagggcct tacaaaaaac gccagaattt aggcatttat cgtcagcaac ttatcggcaa
      601 aaacaaactg attatgcgtt ggctttccca ccgtggcggt gcgttagact tccaagagtg
      661 gaaagccgaa aacccgggta aaccgtttct ggtgtcggtt gcgattggtg cagatccagc
      721 cactatttta gcggcagtca cccctgtgcc ggatacgctt tcggaatatg cgttcgcagg
      781 gctgttgcga gggcataaaa ccgaagtggt caaatctatt tccaatgagt tagaagtgcc
      841 ggcaagtagc gaaattgtgc tggagggcta tatcgatctg gaagaaaccg cattggaagg
      901 cccttatggc gaccacaccg gctactacaa cgagcaagag tattttccgg tatttaccgt
      961 tacccatatt acgatgcgaa aagatgcgat ttatcattct acctacactg gcagaccacc
     1021 tgatgaaccg gcagtattag gtgaagcatt gaatgaagtc tttattccga ttttgcaaaa
     1081 acagtttccg gaaattgtcg atttctactt gccacctgaa ggctgttctt accgtttagc
     1141 ggtagtgacg atgaaaaaac aatatgccgg acacgctaaa cgggtgatga tgggagtttg
     1201 gtcgttcctg cgtcaattta tgtacaccaa gtttgtgatt gtgtgcgatg acgatgtgaa
     1261 cgctcgagat tggaaagatg taatttgggc gatcaccacc cgttgtgacc cggctcgaga
     1321 tacgacactg attgaacaca cgccgataga ttatctcgat tttgcctcgc caattgcagg
     1381 gttaggctca aaaatgggga ttgatgccac caataaatgg gcgggtgaaa ccacccgaga
     1441 atggggaaca cccatcgaaa aagatgacaa tgtggtgaaa cgcattgatg agatttggga
     1501 ttctcttggc atttaaggca acatttaacc gcttgtgcaa cataacaagc ggttaaattt
     1561 tttgacattt ttaccattcg gctatttgtt tctcatcagg ttacgactct ttattttgag
     1621 ccagccactc ggtaggcgaa agccctaatt cccgcataaa gacacggctg agagcagcat
     1681 tatggctata accgacttgc tctgccacca atgctactgg tatccctttt tgtaataaat
     1741 gctgggcaac cgctagtcgc cagttagtga gataatccat cggtgaaacc cccatcgttt
     1801 gtttaaaata agcagcaaag cgtgagcgag acattaatgc cacatttgcc aactcctcta
     1861 cattccaatc tcgctctggt gcattgtgaa tagctaacag cactttcccc agttgcttat
     1921 ctgccaagcc ttttaacaaa cctgtttgaa tatacccttt ttccaaacat tggcgggcaa
     1981 ctttcaaggt aaaataggca cataaatgat gaatcattgc ctgataacca caccctttct
     2041 gattcacttc actaaaaatt tgatcggcta atacccccaa ttccggatac tgtgccaact
     2101 ctaagatcac aatattgtgc aaggtatgag ttaatgggtt gcgaatccct ttgccaaaat
     2161 caaaattaat acaaaatata tctaacccac tatcatctaa cgggtgcaaa ctatgcaaaa
     2221 tattggttgg tgaaaaaata atcgctgggc gttcaatcgt aatcgggcgt tcggcagcct
     2281 gatttaaaat aaaacgtccc tgacgaacga aatgtaaata gcctttattc tcttcattaa
     2341 aactcccgat ttggcataac tgacctagat gaaataaatc cgtgcgaaac tgaaattggg
     2401 taaaaagatg agtcaaacta tccacattaa gctccttggg taaagaattg agacgaacgg
     2461 ttaaaacagg agaaatgcga tcactataat agcaacccgt tcggtaacgc aagttatcat
     2521 tacaatttgt atatctatta atgaggataa aatgatgtca caggaacaat ttgaagaaat
     2581 caaccaagaa cgccgtcaag cccttaacca cggctctcgt attgcaatgg cagccagtgt
     2641 attaggtgca ttaggtattg catcaacagc aaatgcaaaa caagccagtg cagaaaaatc
     2701 accttatgcc gagccggaac aatacggttt aaaacgccat ggaatgacca ttgatccaaa
     2761 acgtgtggca ttagtggtag ttgatccaca aattgacttc ctaggcccga aaggcgtgat
     2821 gtggggcgtg ttaggtgact caattaaaga aaacaacact gtagctaaca ttgaaagctt
     2881 atttaaagca gcaaaagcag tcgatatgcc aacctttgtt tcgccacact actattaccc
     2941 gactgatcac aaatgggaat ttggttcacc gggtgaacac tttatgcacg acagcggaat
     3001 gtttgcgaga aaaggtcaat acaccatgga aggctttgaa aattcaggtg cagactttat
     3061 gcctgagtac aaaccataca tttttgatgg caaaacagtg attacctctc cacacaaaat
     3121 ctttggaccg gaaaccaatg acttagtgtt gcaattacgc aaacgtaaaa tcgaccaagt
     3181 aattttagcc ggtatggctg ccaacttatg tattgattca cacttgcgtg aattaatcga
     3241 acaaggtttt gaagtaacag tggtgaaaga tgcaaccgca gggcctcgta ttcctgaagg
     3301 tgacggctac cttgcagcat taaccaacta ccgtattatc gcaaatgacc tctggaccac
     3361 tgaagaagca gtgaaaaaaa tgacggcaaa agcataatat tcgctttaca agcggttaaa
     3421 tttagctctt tttttgcaaa tttaaccgct tggatttcac gcttatctcc agcactcaca
     3481 ctaaatctca ttatttttcc ttatcacccc attcattttc attatattca tctccacaac
     3541 aaataacagc taccatacaa caattacctt gcctttttca gggttaaaat gcgataatga
     3601 ctcgttattt ttggcttgaa aaagcaatag aaaattgcaa aaaataaagc aaaaataacc
     3661 gcttgtaata gagataaaca agccttaagt gattgatttt gcagtttctt taagacaaga
     3721 caaagtaaac gtaagacaac catcaatgca ctatcgggaa tgaagacagt cgtgagatag
     3781 acattaaatc cattccctta ttaatgggtc aaaatgaccg tgtaagacaa aaacaaaata
     3841 atgagaaaac acaatgaaaa gaatgttaat caatgcaact caaaaagaag agttgcgtgt
     3901 tgccctcgtt gatgggcaac gcttatttga cttggacatt gaaagcccgg gtcacgaaca
     3961 gaaaaaatcc aatatctaca agggtaaaat tacccgtgtc gaaccaagcc ttgaagctgc
     4021 attcgtagat tacggtgcag aacgccacgg cttcctacct ttaaaagaaa tttcccgaga
     4081 atacttcccg tcagactatg tgtttaacgg tcgcccgaat atcaaagata ttattcaaga
     4141 agggcaagaa gtgattgttc aagtcagcaa agaagagcgt ggtaacaaag gagcagcctt
     4201 aaccaccttt atttcgcttg cgggcagtta cttagtctta atgccgaaca accctcgtgc
     4261 cggtggtatt tcccgccgta ttgaaggtga tgagcgttta gaactcaaag aggctttaga
     4321 ctgcttagat gtaccggaag acgtgggctt aatcgttcgt accgccggtg tcggcaaatc
     4381 gccggaagag ttacaatggg acttaaaagt cttgttacac cactgggaag cgattaaaaa
     4441 agcggcggaa tctcgcccag ctccgttctt aattcaccaa gaaagtgacg tgattgtgcg
     4501 tgcaattcgt gactatctac gccgtgatat tggcgaaatc ttaatcgaca acaaaaaagt
     4561 gttcgagaaa gccaaaaacc acattcgcct tgttcgcccg gatttcatca accgtgtgaa
     4621 attatacgaa ggcgaagtac cactattcag ccactatcaa attgaatcgc aaatcgaatc
     4681 ggcattccaa cgtgaagtgc gtttaccttc cggtggctct atcgttattg atgtaacaga
     4741 agccttaaca gcaattgata ttaactcctc acgttcaacc cgtggcggcg atattgaaga
     4801 aaccgctctt aacaccaact tagaagcggc agatgaaatt gcccgtcagt tacgtttgcg
     4861 tgacttaggt ggtttgattg tgatcgactt tatcgatatg acaccggttc gccaccaacg
     4921 tgaagtagaa aaccgtattc gtgaggcgac acgccaagac cgtgcgagaa tccaatttgc
     4981 ccgtatttcc cgtttcggtt tgttagaaat gagccgtcag cgtttaagcc catcattaag
     5041 tgaggcttca tcgcatatct gccctcgctg tcagggaact ggtaaagtac gtgataatga
     5101 atcaatcgca ctgtcaatct tacgtttatt agaagaagag gcaatcaaag aaaacactgc
     5161 acaggttcac accattgtgc cggtgcaaat cgccagctac ttactcaacg aaaaacgtaa
     5221 agcgatttcc agcattgaaa aacgccacga tgtacaaatt gttgtagtac cgtctgaaag
     5281 tatggaaacg ccgcatttca gcgtatatcg cctgcgtgaa aatgaaatta ctccaacgtt
     5341 aagctacgat ttaaccaagc attacgaagc tcgtcatgaa gaagaacacc cacatcatca
     5401 ccatcaagaa gatgtgctgg ttacccgtaa cgagccggtg attacggtag agtcagtgtt
     5461 acaaagctcg gagctgaatc tccaaccttc gccaacacca accgaaccac caaaaccatc
     5521 gctgttaagc cgcttaattg cgaccattaa aggctggttt agtgaagaaa aagtggaaga
     5581 aaaaccgaag ccgaaagcac gcaatcagcg tgagcgtcgc caacgccgtg aacgcccgaa
     5641 ccgtaatgct aaagcggaaa atcaagagcg taaaactccg caagctcaaa ctactccagc
     5701 ggaagaaaaa ccgatgcgta gcgagaaaaa agcacgccga gcggtagtgg aagaagtttc
     5761 cggtgtaacc gagcaagaga gtcgtagcaa ctcccgtgag gttcaaactg aacgtcgcca
     5821 acgccgtgat ctacgtaaaa aagtgcgtat cgaaacacaa gaagtagaag tggaaaatct
     5881 aacacctgct gttgaggaaa cggtggcaaa acaagttgaa aatgtgcaaa aaaatcaaca
     5941 aattcaaccg cttgcaaccc aagaagtacc tgttgaaaca gctcctgagg tagaaacaac
     6001 gaacgaagga cgtgaaaata accgccaacg ccgtttacct cgccatttgc gtgtgggcaa
     6061 tcagcgtcgt cgtgaaaatc aacgcaatca ggttatttct cctatgcctc taacttcagc
     6121 tgttgcttcg cctgagcttg cgagcggtaa agtttgggtt caggttacct ctactgtcaa
     6181 agtagaggaa aaagcatcat tcttatctgt tgatcaaatg cttgaacagc aagaaaatca
     6241 gcttgttgag acagagaaaa aatcagaaga gaagaagctt catacagtta gtccgctagg
     6301 tggctttgta gcacaagcaa ctaaccgtga tttggttaag gagaagcaag aacagctttc
     6361 caaaccgaaa gaagaaaaag ttgcttctga agacgtaaaa ccagtagagg taaaagagca
     6421 tattgctcca tttggtagtg ttgttgctca gcattctgaa cgtgatttag ttaaagagaa
     6481 aaatgagcga gtcagtaaat tccaatcgca agcaacggtt gcaccagcga ttgtggaaga
     6541 ggttactaac ccgattgaag tgccaaaatc aacttacgaa agcgattatc gatttaacgg
     6601 cagattaggt acattctctc gtgttgagca tacccaagcc gctatgacca ttgcttctgc
     6661 accaagtgac aagcttgaag tagtcgaaat tagccaatgg caaaactcta aatactattt
     6721 ctatggcaaa gggtttggcg gatataccag cgcagtgagc caagtgcagt cagcgcctcg
     6781 ttcaaccaat ggttaagtac aattaattgg tttaaatgcc tattagttat aactaatagg
     6841 cattttttta tcaaagtatg aactttctat caatttttct gcctaagtaa acgcacttta
     6901 ttacaaagtg cgttattttt agtttattta aggaactcgg tatgaaaaaa tcatttttat
     6961 cattattagt gttaggtaca actatggcac ttgctgcttg tgatcaggca aaagaagccg
     7021 cgcaatcaac tacagacaaa gcggttgaag taaaagaagc tgtagctgaa aaagctgcag
     7081 aagttaaatc ggctactgaa gcgaaggttg aagagttgaa agcggcagca aatgatgcag
     7141 ctcaagcaac agcagataaa acagaagagg caaaaaaggc agtggcagag aaagccactg
     7201 agttaaaaac cacaactgaa gctaaagtgg aagaactaaa agcagcagca aatgatgcag
     7261 ctcaagcaac aacagataaa acagaagagg caaaaaaggc agtagcagag aaagctgctg
     7321 agttaaaagc cacaactgaa gctaaagtgg aagaactaaa agcagcagca agtgatgcga
     7381 ctcaagcaac agcagataaa gcagaagaag ctaaaaaggc agtagctgag aaggcagcag
     7441 aagtgaaagt tgagacagaa acaaaagttg aagaaatgaa aaaagcgatt gaaagcaaat
     7501 agtgcttttt agtttctctg tagctatcga aacacgctaa aaataa
//
LOCUS       DANLXS010000109         7534 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.109, whole genome shotgun sequence.
ACCESSION   DANLXS010000109 DANLXS010000000
VERSION     DANLXS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.109"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(168..479)
                     /locus_tag="PXD71_001771"
     CDS             complement(168..479)
                     /locus_tag="PXD71_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6291869.1"
                     /translation="MSETEKQAINAPNAVMNGYLTMHYPDWFKPDGIYFNDGAFESFE
                     SSHKLTKDGKIRLVPTAGHTLGHLAVVVDMGEHYILIGGDASYSEQDMLAGNIDGVCN
                     A"
     gene            complement(486..878)
                     /locus_tag="PXD71_001772"
     CDS             complement(486..878)
                     /locus_tag="PXD71_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6291870.1"
                     /translation="MIKVHCLTTGWVQIKIHHQVARFFARPARVLDVLTDKKWSPKLP
                     IGCWLIEHDDGLILVDTGESSRANDKGYQPWWHPFMQFCERRGVKKHEEVGEILKANG
                     FDPLRIDTVVMTHMHGDHAGGLPNFPNS"
     gene            1010..1570
                     /locus_tag="PXD71_001773"
     CDS             1010..1570
                     /locus_tag="PXD71_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL6291871.1"
                     /translation="MNDLRVIKTHLAIQNALIELLDEKLFEKVQVQEIIEKALINRTT
                     FYRYYSGESDLVGKLIKEVKAEYQDLLKKRFESGNLLRFMQTIGNRLLEKRRLILALW
                     QIKTPRHHLYDDMHMLLKNAFIAHAKRITSDDKNWAEKNWDYQAHIFATIALESHKYY
                     FEQGKLLPIPQVFEEWIEMVQVLKSV"
     gene            complement(1636..2625)
                     /locus_tag="PXD71_001774"
     CDS             complement(1636..2625)
                     /locus_tag="PXD71_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL6291872.1"
                     /translation="MSKQIQFTKTGSPDVLQIVDVAIPAPKAHEVQIQIQAIGLNRAE
                     MMYREGAYVIDPVFPATLGYEGAGVIVAIGEGVNEVAISDKVSIIPSFMFTEYGTYGE
                     IVNMPKHAVVKHPDNLSMEQASASWMAFVTAYGGLIEFGKVQKGDFVVLGGATSSVGL
                     ASIQIAKMQGATVIALSRTHAKGDVLLEKGADFVIATSEDDVTAKLFEITNGKGVNVV
                     FDPVGRKEAAKIINAMAQDGRYIIYGALSHDDIAVPVFPILGKHLTVRGYELFEITTV
                     PEKLAQAKTFVYDGLASGQLRPEIDKIFAFDEMVKAHEYMASNQQIGKIVVKV"
     gene            complement(2839..3756)
                     /locus_tag="PXD71_001775"
     CDS             complement(2839..3756)
                     /locus_tag="PXD71_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6291873.1"
                     /translation="MTFIIGILAGVLIAFQSAINARLRMHVGAAFLTSFVSFSVGLLF
                     LLGLSLIMQQTPAFSLDNLGNVPLWAWTGGLLGMVGLTANVLIFPKLGGVQTAVMPIL
                     GQVLMGVLIDTFAWLNSPYIAFTASRLFGIGLVLFGVWIAVVLPHLSGLKRKENANLW
                     LWRAVGILGGTALATQAAVNGEFGRQPGSSLGAATVSFGVGAIGLLLVVLFYEKSLPK
                     LCQPTEGKPFWIWSGGILGSLFILAGVWLVPQIGTGSVVMLVLSGLICGSLLVDKFGL
                     FGITQKPILPLQIIGVTLLLAGVAFIRLL"
     gene            complement(3892..4551)
                     /locus_tag="PXD71_001776"
     CDS             complement(3892..4551)
                     /locus_tag="PXD71_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6291874.1"
                     /translation="MQFFNKEDVINAFHYRASTRSYDGSRKIPAEDFNYILELGRLSP
                     SSVGSEPWQFLVLQNPELRQKIKPYCWGIPTMETSSHIVVILAKKNARYDSQYFSEIM
                     DRRGLQGEAREKAMAVYKKFQEKDIGILDSERSLFDWASKQTYIALGNMMTGAALIGV
                     DSCPIEGFAYQEVNDILAAEGLFDPSEWGVSVMCTFGYRDKEIRQKARKDFETVVKFI
                     E"
     gene            complement(4736..5800)
                     /locus_tag="PXD71_001777"
     CDS             complement(4736..5800)
                     /locus_tag="PXD71_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HDL6291875.1"
                     /translation="MAKFRKLFTPFNIKNLALKNRVVMPPMCQYSATNGVPNDWHFVH
                     YTSRAVGGVGLIIVEMTNVAPNGRITPNCLGLWNDEQQQAFKKLVDSVHAQGAKIGVQ
                     IAHAGRKAQDEPDAVAPSAIHYGELDYAGQNLITPRELTTDEVKALVQAFKDSVKRAV
                     EAGFDTIEIHGAHGYLIHQFYSPKSNQRSDEYGQDKMLFGEQVIQAAREVMPSEMPLI
                     VRISAQEYGENGFGLDYGLQVAKRFADAGADVLDISGGGDGVLHAGNHPEFYAGYQVD
                     FARQVKLATGKPVIAVGMLDNPAVGDHILGMGDADLVAVGRALLRDPYWVLNAQYDQN
                     AADSSEMQFVPKQYERGLFM"
     gene            5891..6799
                     /locus_tag="PXD71_001778"
     CDS             5891..6799
                     /locus_tag="PXD71_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6291876.1"
                     /translation="MSDIRTLDFNLLKAFVVLLDECNVSRAAQRLSITQPAMSGILNR
                     LRESFNDPLFVRVQHGMQPTDRALQLGQTARNILQDISSMLQPPILEPEHLNMTLRIA
                     AMDYVQQIIALPLILRLRRLAPNVKVALLPVQGQNIKTLFEQKKIDLALVSRQHLSTD
                     TLKTVLYEERYVCAMSKTHPMAQTPLSLDQFCNLPFAMLSYNGGEFSGATDLALQKIG
                     LQRKVMVSVNHISLLPQLLQGSDLVAVLPERLAKTLPNVYLQPPPIEVEGFTMMMAWH
                     ERTEQDTAHRWLREVLQGVVKPKQAL"
     gene            complement(6869..7420)
                     /locus_tag="PXD71_001779"
     CDS             complement(6869..7420)
                     /locus_tag="PXD71_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6291877.1"
                     /translation="MAKQIAVLIGSGSKTSFSHLTVRHLQKMAPASIQLNIVDIADLP
                     LYDRDLDENSPAQYTRVREAVAVADGVLLVTPEHNASFSAMLKNAIDVVSRPMGQSKW
                     IGKPAGIVTVGASPAGGVRAADQVRTITSGSFVNMPTLPFALNVGNLFTGVFDEQGEI
                     ASETLKSSMQNFINAYAQFVEKY"
BASE COUNT         2073 a         1748 c         1608 g         2105 t
ORIGIN      
        1 atgctcactt aagtttgaaa tatttttaat aaaaatgacc gcttgccttt caacaagcgg
       61 tcattcccct ttaatttttt accaccgtaa acaccttatt tttcagccga tattcacttt
      121 tttcatcgtg agccagttgc acaatggttg gcttgcgttt acacaattca cgcattgcac
      181 acgccgtcaa tgttgccagc aagcatatct tgttcagagt aagaggcatc gccgccaatc
      241 aaaatataat gctcgcccat atccaccacc accgccaaat gccccagcgt atgtcctgcg
      301 gttggcacaa ggcggatttt gccgtctttg gtgagtttat gactgctctc aaaactttca
      361 aacgccccat cattaaaata gatgccatca ggcttaaacc aatcaggata gtgcattgtt
      421 aaataaccgt tcatcaccgc attgggggcg ttgattgcct gtttttcagt ctcactcatc
      481 acaaatcagc tgtttgggaa attgggcaat ccgcctgcgt ggtcgccgtg catatgggtc
      541 attaccaccg tgtcaatgcg taaagggtca aagccgtttg cctttaaaat ttcgcccact
      601 tcttcgtgtt ttttgacccc acgccgttcg caaaactgca taaacgggtg ccaccacggc
      661 tgatagccct tgtcgttggc acgactgctt tcgcccgtat ccaccaaaat caaaccgtca
      721 tcgtgttcaa tcagccagca accaatcggc agtttaggcg accatttttt atcggtcagc
      781 acatcaagca cacgggcagg acgggcaaaa aagcgtgcca cttgatgatg aattttgatt
      841 tgtacccaac ctgttgtcag acaatgcact ttaatcattt ttgtgttctc attaaaaaat
      901 cgtgggcata ttgtagaaag tgcggtaact tttcacaaac attaaaaagt tcgtatttgg
      961 ctggatatgc agataaaatt acaaaaatgt atgcttattt tgaaaaaaga tgaacgattt
     1021 aagagtaatc aaaacccatc tagcaatcca aaacgctttg attgaattat tagatgaaaa
     1081 gctgtttgaa aaagtgcaag tccaagaaat cattgaaaaa gccttgatta accgcaccac
     1141 tttttaccgt tattattcgg gcgaaagtga tttggtcggc aaattgataa aagaggtgaa
     1201 ggcggaatat caagacctgc tcaaaaagcg ttttgaaagc ggtaatttgc tgcgatttat
     1261 gcaaaccatt ggcaaccgct tattggaaaa aagacggctg attttggcac tttggcaaat
     1321 caaaacccct cgccatcatt tgtatgacga tatgcacatg ctgttaaaaa acgcctttat
     1381 cgcccacgcc aaacggataa catcagatga taaaaactgg gctgagaaaa attgggatta
     1441 ccaagcccat atttttgcca ccattgccct tgaaagccat aaatattatt ttgaacaagg
     1501 caaacttttg cccattccac aggtgtttga ggagtggata gaaatggtgc aggtgttgaa
     1561 aagtgtttag aaataactgg taaaattttc cataaaaaac accgctttca tcacaaaagc
     1621 ggtgctttca aatcatcaaa ccttaaccac aatcttacca atctgctgat tgcttgccat
     1681 atattcgtga gctttaacca tttcatcaaa ggcaaagatt ttgtcaattt cagggcgtaa
     1741 ctgaccgctt gccaaaccgt catagacaaa ggttttggct tgtgccaatt tttcaggtac
     1801 ggtggtaatt tcaaagagtt catacccccg cacggtcaaa tgcttaccca aaatcgggaa
     1861 cactggcaca gcaatatcat catggcttaa cgctccataa atgatgtaac gaccatcttg
     1921 tgccattgca ttgatgattt ttgccgcctc ttttctgccc acagggtcaa ataccacatt
     1981 gacgcctttg ccattggtga tttcaaaaag tttggcggtt acatcgtctt cgcttgtggc
     2041 aatcacaaaa tccgctcctt tttccaaaag tacatcgcct ttggcgtggg tgcgagacaa
     2101 ggcaatcacg gttgcccctt gcattttggc gatttggata ctggctaaac caaccgaact
     2161 ggttgcaccg cccagcacca caaaatcgcc tttttgtact ttgccaaact caatcagtcc
     2221 gccataagcg gtaacaaacg ccatccaact ggcggacgct tgttccattg acaaattatc
     2281 agggtgcttg accacagcgt gttttggcat attgacgatt tcgccataag tgccgtattc
     2341 ggtaaacata aaggacggaa taatgctcac cttatcgcta atggcaactt cgttcacacc
     2401 ctcgccaatc gccacaatca caccagcccc ttcataaccc aaagtcgcag ggaatacagg
     2461 gtcaatcaca tacgcccctt cacggtacat catttcggca cggtttaagc caatggcttg
     2521 gatttggatt tgtacttcgt gtgcttttgg tgcaggaatt gctacatcaa cgatttgcaa
     2581 gacatcaggc gaacctgttt ttgtaaattg gatttgtttg ctcattgttt tgaccttttg
     2641 tgtaaataat gggcgtattt taatgcaatg aatttatttt aaacacaaca tcaattgcaa
     2701 aacacttttg ctaaatttac ataaatacat tttcacaatg tctgaaaagt tgttttgcaa
     2761 attttaaaaa gaaaaccacc gcttgccgat cacaagcggt ggtttttgtg ggttattttg
     2821 caagaggtgt cagcttggct acagtaagcg gataaatgcg acgccggcta agagtaaggt
     2881 aacgccgatg atctgcagcg gtagaatggg tttttgtgta atgccgaaca gaccgaattt
     2941 atctaccagc aggctgccgc aaatcaggcc agagaggacg agcatcacca ccgagcctgt
     3001 gccgatttgg gggacgagcc aaacgccagc gaggataaac aggctgccca gtatgccgcc
     3061 gctccatatc caaaacggtt tgccttcggt tggctggcag agtttcggca ggcttttttc
     3121 ataaaacagc actaccagta ataagccgat ggcaccgaca ccgaaggaga ctgttgctgc
     3181 gccgagtgat gagccgggct gtctgccgaa ttcgccatta acggcggctt gggttgctaa
     3241 tgcggttccg cctaatatgc cgactgcgcg ccacagccat aaatttgcgt tttctttgcg
     3301 ttttagaccg gacagatggg gcagtaccac ggcaatccat acgccgaata agactaaacc
     3361 aatgccaaac aggcggctgg ccgtgaaggc tatgtaaggc gagttaagcc aagcgaaagt
     3421 gtcgatcagc acgcccatca gcacttggcc taaaatcggc atgaccgcag tttgtacgcc
     3481 gccgagcttg gggaagatca gtacgtttgc cgttagcccc accatgccga gtaatccgcc
     3541 cgtccatgcc cataagggga cgttgcctaa attgtcgagg ctaaatgctg gtgtttgttg
     3601 catgatgagc gagagtccga gtagaaacag gaggcctacg ctgaaagaga caaacgaggt
     3661 taaaaatgcc gcacccacgt gcatacgcaa acgggcgttg atggctgatt gaaatgcaat
     3721 cagcacgccg gctaaaatac cgataataaa tgtcatgact aactgggttg tgctgccgcc
     3781 tcaaaatgag tggtggttga aaagaaatgc cgtctgaatg cggattcaga cggccttaag
     3841 gcttggtatc ggcgtttacc tgatgttgaa tagccgtttt gcctagccgg tttattcgat
     3901 gaatttcacc acggtttcga aatctttacg tgctttttgg cggatttctt tatcacggta
     3961 accgaaggta cacatcaccg atacgcccca ttcgcttggg tcaaataatc cttccgctgc
     4021 caaaatatcg ttgacttctt ggtaagcgaa accttctatc ggacaagaat ctacgccaat
     4081 taaagctgca ccggtcatca tattgcccaa tgcgatgtag gtttgtttgc ttgcccagtc
     4141 aaacaggctg cgttcgctgt ctaaaatgcc gatatctttc tcttggaatt ttttatacac
     4201 ggccatggct ttttcgcgcg cttcgccttg taaacctcgt ctatccataa tttcagaaaa
     4261 atattggctg tcgtaacggg catttttctt cgctaaaatc accacgatat ggcttgaggt
     4321 ttccatagtt ggaatgcccc agcagtaagg tttgattttt tgccgtaatt cagggttttg
     4381 caacactaaa aactgccaag gctctgagcc taccgaactt ggacttaaac gaccgagttc
     4441 cagaatatag ttgaaatctt cagccggaat ttttctgctg ccgtcgtaag aacgggtaga
     4501 tgcacggtaa tgaaaggcat tgattacgtc ttctttgttg aaaaattgca tggaacactc
     4561 ctaaaaatgc aaaacagtct tttcagacgg tctgaaaagt ctagatgaaa tacggataag
     4621 tataaaagat ttaagttatt tttaataatt gataattttg atatttaggt ataagttgac
     4681 gtgatgagaa aaaacagacc ctctgaatgt tcaggcggtc tttaacgttg gttgtttaca
     4741 taaataatcc gcgttcatat tgcttcggta caaactgcat ttcactgctg tctgctgcat
     4801 tttggtcata ttgcgcattc aatacccagt acggatcgcg caataaggct cttcccacag
     4861 ccactaaatc cgcatcgccc atacctaaga tatgatcgcc taccgccgga ttatccaaca
     4921 tacctactgc aattaccggt ttgcccgtag cgagcttcac ttgacgggca aagtctactt
     4981 ggtaacctgc gtaaaattcg gggtgattac ctgcatgcaa tacaccatca ccaccaccgc
     5041 tgatgtctaa gacatctgcg cctgcatcgg caaaacgttt cgctacctgc aaaccataat
     5101 ccaagccaaa gccgttttca ccgtattctt gtgcagaaat gcggacaatt aacggcattt
     5161 cagacggcat cacttcacgt gctgcctgaa tcacttgctc gccaaacaac attttgtctt
     5221 ggccgtactc atcactgcgc tgattggatt tcggcgaata gaattggtga attagataac
     5281 cgtgtgcgcc gtggatttca attgtatcaa agcctgcctc aacggcacgt ttgaccgaat
     5341 ccttaaaggc ttgaaccaat gctttgactt cgtctgtcgt cagctcgcga ggtgtaatca
     5401 gattttgtcc ggcataatcc aactcaccgt agtgaattgc acttggagcc actgcatccg
     5461 gctcgtcttg ggctttgcgt ccggcatggg cgatttgcac accgattttt gccccttgag
     5521 catgaacgct gtctaccagt tttttgaatg cctgttgttg ttcatcgttc cataagccta
     5581 agcagtttgg cgtaattctg ccgtttggcg ccacattggt catctccaca ataatcaagc
     5641 cgacaccacc tactgcacgg ctggtgtaat gcacgaaatg ccaatcgttc ggtacgccgt
     5701 tggttgccga atattgacac attggtggca taaccacacg gtttttcaat gctaagtttt
     5761 taatgttaaa cggggtgaat aattttctga acttcgccat gttgccttcc ttaatgaaat
     5821 agattaggtg gaatgataat tcagtgctat tatctattca aattgattat tgtgatagtt
     5881 aggtataaaa atgagtgata taagaacact ggatttcaat ttgctcaaag cttttgtggt
     5941 gctgctggac gaatgcaacg ttagccgggc agcacaacga ttatccatca cccaaccggc
     6001 aatgagcggt attttaaacc gcttgcgcga gagttttaac gatccgttat ttgtgcgagt
     6061 gcaacacggt atgcagccaa ccgaccgcgc gttgcaactg gggcaaaccg cacgcaacat
     6121 tctgcaagat atcagctcca tgctgcaacc acccatattg gaaccggaac atctgaatat
     6181 gaccttgcgt attgccgcaa tggattatgt acaacaaatt attgccttgc cgctgattct
     6241 gcgtttaaga cggcttgctc cgaatgtaaa agtcgcctta ctgccggtgc aggggcaaaa
     6301 tatcaaaact ttgttcgagc aaaaaaaaat cgatcttgcc ttggtcagcc gccaacattt
     6361 aagcacggat acgctgaaaa cggtattata tgaagaacgc tacgtatgcg cgatgagcaa
     6421 aacacacccg atggcgcaaa caccgctatc gctcgatcaa ttctgcaatc ttcccttcgc
     6481 aatgctttct tacaacggcg gagaattcag cggagctacg gatctagcgt tacaaaagat
     6541 aggactacag cgcaaagtaa tggtctcggt aaaccatatt tccctgctgc cccagctgct
     6601 acaaggttcc gacttagtcg cagtattgcc cgaacgctta gcaaaaacct tacccaatgt
     6661 ttatttacaa cctccgccta tagaggtaga aggcttcacc atgatgatgg cttggcacga
     6721 aagaaccgaa caagatacgg cgcatcgatg gttaagagag gtgctgcagg gtgtggtaaa
     6781 acctaaacag gctctttaat tattctttct tttgctatga aaaaagccgt ttatattttt
     6841 agataaacgg cttttttgca ttctaatatt agtatttttc tacaaactga gcataggcat
     6901 taatgaagtt ttgcattgag ctttttaatg tctcagaggc aatttcaccc tgttcatcaa
     6961 atacgcctgt aaataagttt cctacgttta atgcgaaagg taaggttggc atatttacaa
     7021 atgagcctga agtaatagta cgtacttgat cagctgcacg tacaccgcct gccgggcttg
     7081 cgccaacggt tacgatacct gccggtttgc caatccattt gctttgcccc attgggcgag
     7141 aaaccacgtc aatcgcattt ttaagcattg cagaaaagct tgcattatgc tcaggtgtga
     7201 ctaataacac gccatctgcc acagcaaccg cttcacgcac tctggtgtat tgtgccgggc
     7261 tgttttcatc taaatcacgg tcgtaaagtg gtaagtctgc aatatccaca atgtttaatt
     7321 gaatgcttgc aggtgccatt ttttgtaagt gacgaacagt caagtgacta aatgaagttt
     7381 tgctgccgct accaattaat actgcaattt gttttgccat tttataattc cttagtacat
     7441 agagaatata ggcattttat ccctttcaat ctgtttcata aagcgcttat gatgaaaagc
     7501 actatttact tatattaaat aattttgcaa attt
//
LOCUS       DANLXS010000110         7531 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.110, whole genome shotgun sequence.
ACCESSION   DANLXS010000110 DANLXS010000000
VERSION     DANLXS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.110"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..696
                     /locus_tag="PXD71_001780"
     CDS             <1..696
                     /locus_tag="PXD71_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL6291878.1"
                     /translation="NIILLWLLALILFSLGYKYAAYFFALLGILILYIFSIPYTSQKL
                     GDSLVEAEPFTIEKYKNAQAIVVLGGGLRDSQELFGKLAITGIPLERMRYAAYLHKET
                     GLPILATGSSPNGTSEAKVMADEFKMFFNIETKWLENKAKTTKENAIFSREILEKENI
                     HKIILVTNQWHMKRAEMLFSQQGFEVMSASVGSGDTPDHYKLNFMHFIPQAGAMNSNM
                     LALKEWIGYWKEK"
     gene            complement(773..2971)
                     /gene="priA"
                     /locus_tag="PXD71_001781"
     CDS             complement(773..2971)
                     /gene="priA"
                     /locus_tag="PXD71_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL6291879.1"
                     /translation="MALNVPLHRYFDYLLPPAFNACQGGRVVVPFGRQTKIGIVVDFP
                     QTSDVPTEKLKPIKAALDSSPIFDSEMWQLLNWAARYYHAPIGEVLTGALPIKLRNGD
                     STERVQPNYFVVTEQGRKSLIIGENKKAKKQVERLTELAECAKYFEKPTACSPSAWKG
                     LLEKGYIEKIDVPFMAQAWQDSLGEFPLVNTNNRLILNKQQTLVVSRLNAQTTFASFL
                     LNGVTGSGKTEVYLQLIETVLKREEQVLLLVPEIGLTPQTVQRFKARFNVEIDVLHSN
                     MNETERLNAWLRAKNGESAIVIGTRSALFTQFQKLGVIILDEEHDSSFKQQDGWRYHA
                     RDLAVVRAKNANIPIVLGSATPSLESLHNVQNGKFIELTLTARAGNAQLANQQIIDLK
                     TQRVTAGLSERLLSMMKTQLEQGNQVMLFLNRRGFAPVLLCHECGWVCECEACNKPFT
                     YHQKQKVLRCHHCATQRVIPRQCGHCGSTHLITTGVGTEQLEQVLNQQFPHYQVTRID
                     RDSTARKGALENHLTAIREGKSQILIGTQMLAKGHHFPNVTLVAIVNVDSALFSTDFR
                     AEEKLAQLYIQVAGRAGRAEKKGEVVLQTHYPDHPLLKTLLEQGYRAFADEALKMRQL
                     MSLPPFSSQVLFKATGKDNQQTLQLLEQLADYFSQKIQQQNGQGFQLPPPFSAPMAKK
                     AGHYRWLLLIQHPLRGALQQLLDAFDQERESLNIPNNIRLNIDIDPQEIS"
     gene            complement(2983..3595)
                     /locus_tag="PXD71_001782"
                     /pseudo
     CDS             complement(2983..3595)
                     /locus_tag="PXD71_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(3588..4487)
                     /gene="rarD"
                     /locus_tag="PXD71_001783"
     CDS             complement(3588..4487)
                     /gene="rarD"
                     /locus_tag="PXD71_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL6291880.1"
                     /translation="MLKGILLSLSASVLFGCMYYLAIFLKPLAGVSIFGIRMLVTIPF
                     LFLALYLFKKQNEFWVFLQRLKQEPYLLLVILITASIVGGQMWLFLWAPNSGKAIEVS
                     MGYLLMPIIMVAFGKVVYKETLSLNKWLAIVVAFIGVASNILFTGKISIASLFVCTGY
                     PIYFYLRRKFGLSHLHSFVVEILYLLPVACYFISQTDMQYVETQNLNIYYFIALLGLI
                     SGTALISYTLASTILPFNLLGLLGYVEPLLMLVVSFLIGERLANESYLLMICLLISIS
                     FLVLDGMFALRQQRKRKLSVNNE"
     gene            complement(4714..5931)
                     /locus_tag="PXD71_001784"
     CDS             complement(4714..5931)
                     /locus_tag="PXD71_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL6291881.1"
                     /translation="MDRFPKSDKLAQVRYDIRGPIHKEALRLEEEGHKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIVQYYQSKGMRGMDVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPMPDYPLWTAASTLAGGKAVHYLCDEENEWFPDLED
                     IKSKITPRTKGILVINPNNPTGAVYSRQILLEIAELARQHNLIIFADEIYEKIVYDGA
                     VHHHIAALAPDVLTVTYNGLSKAYRVAGFRQGWMVLSGPKHQAKGFIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFVLPGGRLLEQRNKMHELLVQIPGISCVKAKGALYM
                     FPKIDTEMYGIKDDQKFIYDLLRQEKVLLVQGSGFNWKKPDHFRVVTLPYAHQIEEAI
                     GRLANFLKTYRQD"
     gene            6119..7423
                     /locus_tag="PXD71_001785"
     CDS             6119..7423
                     /locus_tag="PXD71_001785"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HDL6291882.1"
                     /translation="MLIFHQLKQELTKSYSKVGREQGLVAIQASMPLLEANIPLLGWL
                     KTQKTSYPHFFLQYRDTDQTIATIGTLRQFYTLEAAQSFIQQHHFPLIGGLQFEGNTQ
                     FILPQFSLVKNQQNLTACFYFDSDNFEQQAVIFEQFLANFSQITELGLTDNPLVSMSA
                     TSHFEQWKANIEKAIVSIEQGDFQKVVLANAMRLHFEQPICAYSLLAQSQKTNLGCYH
                     FLWAESADVAFLGSTPERLYQRQGLHFSTEALAGTAAVTTSEVQTELNAQWLLSDPKN
                     IYENQLVVDDIENQLKDCVTEFAVSEAKIKRLHNVQHLRRQIQATLKPDTSDSDCLSK
                     IHPTAAVAGLPRQKAKGFITENEPFTRHWYAGTLGVMGNEEAEFCVTLRSALISQNSI
                     TLYAGAGIVSGSDPQSEWQEIKRKAQGMAKLLDVDLVTKGEL"
BASE COUNT         2278 a         1648 c         1483 g         2122 t
ORIGIN      
        1 aatattattt tgctgtggtt gttagcatta atcctctttt ctttaggcta taaatacgct
       61 gcttacttct ttgccttgtt aggtattctg attttatata tcttcagcat tccctatact
      121 tcacagaaat taggggattc attagtcgaa gcagagccat ttaccataga gaaatataaa
      181 aatgctcaag cgattgtggt gctaggtggc gggctacgag acagccaaga gttattcggc
      241 aaactggcaa ttaccggtat tccgctggaa agaatgcgtt atgccgctta tctgcataaa
      301 gaaaccggct taccgatttt agcgaccgga agcagcccaa atgggacttc tgaggcaaag
      361 gttatggcag atgaatttaa aatgttcttc aatattgaga ccaaatggct ggaaaataag
      421 gcgaaaacca ccaaagaaaa tgcgattttc agccgtgaga ttttagaaaa agagaatatt
      481 cataaaatca ttcttgttac caaccaatgg catatgaaac gggctgaaat gctgtttagc
      541 caacaaggat ttgaggtgat gtctgccagc gtcggttctg gcgatacgcc cgatcattac
      601 aaactcaact ttatgcactt tattccgcaa gccggtgcaa tgaatagcaa tatgctggca
      661 ttaaaagagt ggattggtta ttggaaagag aaataaccga agataaatag cagcaaggca
      721 ggttttcacc tgccttcttt ttgcaaaaaa tcagtcaaaa acaaccgctt gtctaactaa
      781 tctcttgtgg gtcaatgtcg atattcagcc tgatattatt cggaatgttt aaactttccc
      841 tttcttgatc aaaggcatcg agtagctgtt gtaatgctcc tcggagaggg tgttgaatca
      901 gcaataacca gcggtaatgt cctgctttct tcgccattgg ggctgaaaat ggtggaggga
      961 gctggaagcc ttgcccgttt tgttgctgga ttttttggct aaaataatct gctaattgct
     1021 caagcagctg taaggtttgt tgattatctt tgccggtcgc tttgaacaaa acttgcgagg
     1081 aaaatggtgg caaactcatt aattgcctca tttttaacgc ctcatcagca aaagctcggt
     1141 agccttgttc cagcaaggtt ttaagcagtg ggtgatcagg gtaatgggtt tgtagcacca
     1201 cttcgccttt tttctccgct cgccctgctc tgcctgccac ctgtatgtaa agctgtgcta
     1261 atttttcttc cgcacgaaaa tcagttgaaa ataacgctga atccacatta acaatcgcca
     1321 ccaacgtcac attcgggaaa tgatgccctt ttgccaacat ttgcgtacca atcaaaattt
     1381 ggcttttgcc ctctcgaata gcggtcaaat ggttttccaa tgcacctttg cgagcggtag
     1441 aatctctgtc tatacgagtc acttggtaat gagggaattg ctgatttagc acctgctcaa
     1501 gttgttctgt gccgacaccg gtagttatta aatgggttga accacaatgt ccgcattggc
     1561 ggggaattac cctttgggtg gcacaatgat ggcaacgcaa cactttttgc ttttggtggt
     1621 aagtaaaagg cttattacac gcctcgcact cacaaaccca accgcattcg tgacaaagta
     1681 ataccggggc aaagccacgg cgatttaaaa acagcatcac ttgattgcct tgctctaact
     1741 gggttttcat cattgaaagc aagcgctccg acaaaccggc agttacccgt tgcgttttta
     1801 aatcaataat ctgctgattg gcaagttgtg cattgccggc ccgagcggtt agtgtcagtt
     1861 cgataaattt cccgttttgc acattgtgca aactttcaag acttggcgtt gccgagccca
     1921 gcacaatcgg aatatttgca tttttcgccc taaccaccgc caaatcccgt gcgtggtaac
     1981 gccaaccgtc ttgttgctta aacgagctat cgtgttcttc atccaagata atcacgccca
     2041 atttttgaaa ctgggtaaat aaagcagagc gagtgccgat tacaatcgca ctttctccat
     2101 ttttcgcccg caaccacgca tttaaccgct cagtttcgtt catattcgag tgcaatacgt
     2161 caatttcaac gttaaagcga gctttaaagc gttgtaccgt ttgcggtgtt agcccgattt
     2221 caggcacaag cagcagcact tgttcctctc gcttaagcac cgtttcaatt aactgcaaat
     2281 acacttcggt tttgcccgag cctgttacgc cattgagtaa aaagctcgca aaagtggttt
     2341 gtgcatttaa acggctgacc actaaggttt gttgtttatt taagatcagc cgattgttag
     2401 tatttactaa cggaaattca cctagtgagt cttgccacgc ttgtgccata aacggcacat
     2461 caattttctc gatataccct ttttcgagta agcctttcca agcagatggg ctacaagcgg
     2521 tcggtttttc aaaatatttt gcacattcgg caagctcggt taaccgttcc acctgctttt
     2581 tggctttttt attttcaccg ataatcagag atttccgccc ttgctccgtc accacaaaat
     2641 aattcggttg aactcgctca gtggaatcgc cattgcgtaa ttttatcggt aaggctccgg
     2701 ttagcacttc accaatcggg gcgtggtaat aacgagccgc ccagttcagc agttgccaca
     2761 tttctgagtc aaaaatcggg ctagaatcca acgctgcttt aatcggtttg agtttttcgg
     2821 ttggcacatc actagtttgc gggaaatcga ccacaatgcc gattttggtc tggcgaccaa
     2881 aaggcacaac cacccgcccg ccttggcagg cattgaatgc cggcggtagc aaataatcaa
     2941 aataacgatg taacggcaca ttcagtgcca ctctgattaa tctcacttct ctaaaccaat
     3001 tttgattgca agcccgataa atatcgagcc gcagatttta tttaaccaat ttccaaaggt
     3061 ttgattatgc cgtaaaaaac gcccaacagg tgcagcggct aagactaaaa tacttaccca
     3121 aataatgctg gtggataaaa acgttagccc caacagcata aaaggtattt tccaaattac
     3181 tggatacaaa ctgcggcaat aacgccaaga aaaagagcaa tactttcggg tttaatagat
     3241 tggtaatcaa cccttgacgg aatatcttcc atagaggaag tttgtctaga ttgattttct
     3301 ctaacaccaa cggctctcgc cacattttaa tgccgagata gcagagataa attgcccctg
     3361 cgtatttcag aaaattaaat aaagtgggcg aatgtgccac aatgcctgca agcccgagtg
     3421 aaaccgcaag aatatggcat aaaatgcctg ctagaatgcc gaatgaagac attaagcccg
     3481 actttttccc ctcagcaata gtgcgagtta aaatatagat agtatccgaa cccggtgtaa
     3541 gatttagcag aatacaagcg gtcaaaaatc cccaataatt tacaatatca ctcattattc
     3601 acactcaatt tacgtttacg ctgctgacga agagcaaaca taccatctaa tactaaaaat
     3661 gaaatggaga tcaacaagca aatcatcaac aagtaagatt catttgctaa cctttcgcca
     3721 attaagaaag acaccaccaa catcaatagt ggctcaacat agcctaataa accaagcaaa
     3781 ttaaagggta aaatcgtgct ggctagcgta tagctgataa gtgcagtgcc actaattaaa
     3841 cccagtaatg caataaaata gtaaatattg aggttttgtg tttcgacata ttgcatatcg
     3901 gtttgagaga taaaataaca agctacaggg agcaaatata aaatttctac cacaaagcta
     3961 tgtaaatggc ttaagccaaa ctttcggcga aggtaaaaat agattgggta acctgtgcaa
     4021 acaaataaac ttgctatcga aatttttccc gtaaacaaaa tattactcgc tacaccaatg
     4081 aaagcaacca caatcgccaa ccatttattc aaggaaagcg tttctttata aaccactttg
     4141 ccaaatgcca ccataataat cggcattaat agatagccca ttgaaacctc aatcgctttt
     4201 ccgctattgg gagcccataa aaacagccac atctgcccgc ccacaataga tgccgtgatt
     4261 aaaataacca gcaataaata aggctcttgc tttaggcgtt gaagaaatac ccaaaactca
     4321 ttttgttttt taaataagta taaagcaagg aaaagaaaag gaatcgtaac cagcatacga
     4381 atgccgaaaa tgctcacacc tgccagcggt tttagaaaga tcgccaaata gtacatacag
     4441 ccgaataaca ctgaggctga aagagataaa agaatgcctt ttaacatagt gataagaaag
     4501 taattatttt ttaatttttc aaatctcccc tacccctctt ttctaaagag gattgtcgag
     4561 ttctaaataa atgaataatg ttcggaggta ggggcgggtc cagtgcccgc ccgcaaatta
     4621 atatcgttag cggacaggca caggacctgt ccctactgat attatagaac ttacaaccct
     4681 gcttttataa agtggggtaa agggagattc gacttagtct tgacgatagg ttttcaagaa
     4741 attcgcaagg cgaccaatcg cttcttcaat ttgatgtgca taaggcaaag ttaccacacg
     4801 gaagtgatcc ggttttttcc agttaaaacc ggaaccttgc actaatagca ctttttcttg
     4861 tcgcaacaaa tcgtagatga atttttggtc atctttaatg ccgtacattt cggtatcaat
     4921 tttcgggaac atatataacg ctcctttcgc ttttacacag ctaatacccg gaatttgtac
     4981 caataattcg tgcattttat ttcgttgttc cagcaaacga ccgcccggca acacaaattc
     5041 attgatactt tgataaccac ctaatgcggt ttgaatggcg tgttgcatcg gcgtattggc
     5101 acataaacgc atagaagaga gcatatccaa cccttcaata aaacctttgg cttgatgctt
     5161 cggaccgctc agcaccatcc agccctgacg gaaaccggca acacgatacg ccttggataa
     5221 accgttataa gttacggtta atacgtccgg tgcaagagca gcaatatggt gatgtaccgc
     5281 accatcatag acaatttttt cataaatttc gtcagcaaaa ataattaagt tatgctgacg
     5341 ggcaagttct gcaatttcca gcaaaatttg acggctatac actgctccgg tcgggttatt
     5401 cgggttgata accaaaatcc ctttagtgcg aggcgtgatt ttcgatttaa tatcttcaag
     5461 atcagggaac cactcatttt cttcatcgca caggtaatgc actgctttac caccggctag
     5521 ggttgaagct gctgtccata acggataatc aggcattgga attagaattt catcgccatc
     5581 atttaataac gcctgcatcg acattgtaat cagttcagat acgccgttgc cgatgtaaac
     5641 atcgtttaca tccataccac gcataccttt tgattgataa tattgcacaa tcgctttgcg
     5701 ggcagaatac aaaccttttg aatcacaata gccttgtgcg gttggaagat tacgaatcac
     5761 atccactaaa atttcatcgg gtgcttcaaa gccaaaaggg gctgggttac cgatatttaa
     5821 ttttaaaatt ttatgccctt cttcttctaa gcgaagtgcc tctttatgaa ttggcccacg
     5881 gatatcgtaa cgaacctgag ctaacttatc tgatttggga aaacgatcca ttgtaaactt
     5941 cctataatgt attatctaat tgtagtctca tcccgctgtt caaaaaatcg gtaattcgga
     6001 atgaatttgt aaacattact ctaatttggg aggaaaaaaa agaggaaata cgttaaaatg
     6061 gagacctttt tttcaatcag ctaaaaacaa gcggtttctt tttctgattt ttttgcaaat
     6121 gcttattttt catcaattaa aacaagaact tacaaaaagt tactcaaaag tgggaagaga
     6181 gcaaggattg gtcgccattc aagcctctat gccgctattg gaggcaaata ttccgctact
     6241 cggttggttg aaaacacaaa aaacaagcta ccctcacttt tttctgcaat atcgtgatac
     6301 agatcagacc attgccacca ttggcacact tcgccaattt tatacgctag aagctgcaca
     6361 atcttttatt caacaacatc atttcccgtt aatcggcggg cttcaatttg aaggcaatac
     6421 gcaatttatt ttgcctcaat tttcattggt aaaaaatcag caaaatttaa ccgcttgctt
     6481 ctattttgat agtgataact ttgaacaaca agcggttatt tttgaacaat ttcttgcaaa
     6541 tttcagccaa attaccgaat taggactaac ggataatccg ctcgtttcaa tgagtgccac
     6601 cagccatttt gagcaatgga aagccaatat tgaaaaagcg attgtttcta ttgagcaggg
     6661 cgattttcaa aaagtggtat tggcaaatgc gatgagactt cattttgagc aacccatttg
     6721 tgcttacagc ttgctggcac aaagccaaaa aacaaattta ggttgttacc actttttatg
     6781 ggcggaaagt gctgatgttg cctttcttgg ctcaacccct gagcggctat atcaacgcca
     6841 agggctacac ttttctaccg aagccctcgc cggcaccgct gcggttacca ccagtgaagt
     6901 gcaaaccgaa ctcaatgcac aatggctgtt aagtgatccc aaaaatatct atgaaaatca
     6961 attagtggtt gatgacattg aaaaccaact caaagattgc gtgaccgaat ttgcagtatc
     7021 cgaggcaaaa attaagcgtt tgcataatgt gcaacaccta cgccgccaaa ttcaagcaac
     7081 gctcaaaccg gacacctcag attcagattg cttaagcaaa atccacccaa ctgcggcagt
     7141 tgcagggctt ccccgccaaa aagcaaaggg ttttattacc gaaaatgagc cttttacccg
     7201 ccattggtat gcaggtacat taggcgtaat gggcaacgag gaagccgaat tttgcgtaac
     7261 actccgttct gcattaattt cacaaaattc aattacgctt tatgccggtg ccggcattgt
     7321 gtcaggctca gatccacaat cggaatggca agaaattaaa cgtaaggcac aaggaatggc
     7381 aaaactgttg gatgttgatt tagtaacaaa aggggaactg taagctaggc tttcagaact
     7441 taatctctta attatttctt tctatatttt catttccaac ttcccaacaa aatatattat
     7501 tgccaccaaa ttagtagtga atattccata a
//
LOCUS       DANLXS010000111         7518 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.111, whole genome shotgun sequence.
ACCESSION   DANLXS010000111 DANLXS010000000
VERSION     DANLXS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.111"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            122..811
                     /locus_tag="PXD71_001786"
     CDS             122..811
                     /locus_tag="PXD71_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL6291883.1"
                     /translation="MSTLSRDSILSQIQNGLIASCQPVDDGPMDKPEIVAAMAAASVI
                     GGAKGIRIEGVDNLKAARAAVNVPIIGIVKRDLPDSPVRITPFLEDIDALAEAGADII
                     AVDGTFRTRPVSIEEAVKRIHEKGCLAMADCSTLEEGLHCQQLGFDIVGSTMSGYTGG
                     EMPDEPDYQLVKDLKAAGCNVMAEGRYNTPELAKTAIEIGADYVTVGSALTRLEHIVS
                     WFADSVKSAQK"
     gene            821..1723
                     /locus_tag="PXD71_001787"
     CDS             821..1723
                     /locus_tag="PXD71_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDL6291884.1"
                     /translation="MRCLAIDIGGTKIATAIVQHNQVSQRQQIQTPTDKPQADQAGSL
                     HQAIGEIMQHYQGQFDFVAVASTGIINKGVLTALNPKNLGGLAEFPLKQSIEKHTDKP
                     IALLNDVQAAVCAEYLNEDQQAVKNFVFITVSTGVGGGIILNGELQIGSNGIAGHIGH
                     TLADPNGPLCGCGRVGCVEAVAAGRAIEAVSSQWEKPCSPKEVFERFRQGNPQAVELV
                     QKSAKAIANLIADLKISLDIQKVVLGGSVGLAEGYLPQVSNYLAEMPSVYHCVLENAL
                     SGQDAGLIGAAWWAENQLNQGFTL"
     gene            1748..2626
                     /gene="nanA"
                     /locus_tag="PXD71_001788"
     CDS             1748..2626
                     /gene="nanA"
                     /locus_tag="PXD71_001788"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDL6291885.1"
                     /translation="MKNLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYV
                     GGSTGENFMLSTEEKKEIFRIAKDEAKDEIALIAQVGSVNLKEAVELGKYATELGYDS
                     LSAVTPFYYKFSFPEIKNFYETIIRETGNKMIVYSIPFLTGVNIGVSQFAELFENEKI
                     IGVKFTAGDFYLLERLRKAFPNHLIYAGFDEMMLPATVLGVDGAIGSTFNVNGIRARQ
                     IFELAQQGKIKEAFEIQNVTNDLIEGILGNGLYQTIKGLLELEGVQAGYCREPMTKEQ
                     TPAQKEVTKQLKAKFL"
     gene            complement(2687..2893)
                     /locus_tag="PXD71_001789"
     CDS             complement(2687..2893)
                     /locus_tag="PXD71_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291886.1"
                     /translation="MDNKQTNGFNDIIVKADITQNIWSTGFYDIGRELKSENTRQEKV
                     RLKYNGDTLHRGRKSRMASLIASR"
     gene            complement(2851..3489)
                     /locus_tag="PXD71_001790"
     CDS             complement(2851..3489)
                     /locus_tag="PXD71_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291887.1"
                     /translation="MKKILLLVLLLTLATQADEGNNTQVCAEQQHQIIAHYFKISQEK
                     LSARTLASACKTAPNNSTETLVSYAVIEPKYSKENYQNDENTPKWTLFVARVNDNAIL
                     QSWEQDMEIDASIDVNADSLKIDTARYILNDKVRAFGVRFNNAARGPSAPEYSANDHL
                     MLFVPEKEKLKLVFDYPMTFWSILDDKETKTETQTWYCKWITNRPTASMILL"
     gene            complement(3731..4450)
                     /locus_tag="PXD71_001791"
     CDS             complement(3731..4450)
                     /locus_tag="PXD71_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6291888.1"
                     /translation="MSKPNIYDESFSERMKWIAKNSFKDNYSEFARAVGVAQASLSRW
                     VKGEADPSRSNLVKIAEVSNVSLEWLATGKESKPQSENTSIVAREFERLEKMADDIVS
                     MINSFRTINVSAGFGSFNEGVTKPDGQEPYADSLLQSLGVKAHNCGVFWANGTSMHPT
                     IADSDQMLVDFSKREPRGDDKIYLVQNGESVWVKRIRKEWDYIELISDNESYRPIQIT
                     AEEAQNLQIIGQVVHIGHSLV"
     gene            4627..4905
                     /locus_tag="PXD71_001792"
     CDS             4627..4905
                     /locus_tag="PXD71_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6291889.1"
                     /translation="MSVLNDVKKTAQRDWHKADILAELRKKGWSLRSLAKAGNVSHNT
                     LKSVLDKPYPKMERLVANAIGVAPEVIWAGRTAERIERNRRPTLTNKF"
     gene            4950..5753
                     /locus_tag="PXD71_001793"
     CDS             4950..5753
                     /locus_tag="PXD71_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6291890.1"
                     /translation="MSELSLKTHYSVAELLSFKLSSLPSAHKNVLEKAIRENWQSQKR
                     KGRGGGVEYELISLPTEVQQEIRTKLLKLLPAEISKGELSVVRQNIDLEQITDKQLST
                     ADARIMVVRWFLMQEVQLGLSRTKTLDQVIAAVASSEIPAEICKAIMAGNSKAGGKLK
                     LSKRTLHSWVLAYEAGENSAERLKQMIPLKTQKRAVPERCGWLQAFLPFYQTFSNVAL
                     TQAYAQFAMQYEGEDLPTESQVRYALKQLPDYVVQQGRKLPVKKIRLAR"
     gene            5720..6232
                     /locus_tag="PXD71_001794"
                     /pseudo
     CDS             5720..6232
                     /locus_tag="PXD71_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            6243..6617
                     /locus_tag="PXD71_001795"
     CDS             6243..6617
                     /locus_tag="PXD71_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6291891.1"
                     /translation="MSIVYEGSCVLEVDGVEIEITKLDVKTVTGRKLVKTMNSSGRAR
                     GYSQGIAEYTLSVTAVEPQDGSVIDWDNIKNAKLTKYPLDNSEKRTSYLGCFTTDVSE
                     SYTVDGESVRDIQLNALRKVNE"
     gene            6617..6985
                     /locus_tag="PXD71_001796"
     CDS             6617..6985
                     /locus_tag="PXD71_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291892.1"
                     /translation="MKITLNLGIEYNGKIRRDCTVRLLTMGGQCTAQEMIADLGFEEN
                     SEALSKREQTLVDMAYLSQQVTFDGIYQGNVTAQFLFDNLATEDYWQLIEATLTLRKK
                     RLENGVSQSESESPANPDLA"
     gene            7015..7203
                     /locus_tag="PXD71_001797"
     CDS             7015..7203
                     /locus_tag="PXD71_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291893.1"
                     /translation="MILLSKFAITAEQVWQMPLLEVFAWIESYLESEGVKTPHSPMGA
                     NSEVKRESYVFTRCGKIE"
     gene            7256..>7518
                     /locus_tag="PXD71_001798"
     CDS             7256..>7518
                     /locus_tag="PXD71_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291894.1"
                     /translation="MAKNMKVQLELTAKDTASPVLNQAAKTAEKAFKNTERAAQKSGN
                     AQTQAAQKAANATESSNRKIEQSYRNARRVAADVSRARETLGVR"
BASE COUNT         2254 a         1508 c         1697 g         2059 t
ORIGIN      
        1 tcacattttt ttatttttct gtttgtagta aaaatttatt aaattataat tatttggagt
       61 attttttcac aaacaaaatc tagtccattg actacctttt gaacataaaa aaggaactgt
      121 tatgtcaaca ctatctcgtg attctatttt aagtcaaatt caaaacgggc tgattgcctc
      181 ctgccaaccg gttgatgacg gccccatgga taaacctgaa attgtcgccg caatggcagc
      241 agcctctgtg attggtggtg ctaaaggcat tcgcattgaa ggtgtcgata atttaaaagc
      301 cgctcgtgcg gcagtgaatg tgccgattat cggcattgta aaacgtgatt tgccagattc
      361 ccctgtgcgt attacgccat ttttagaaga tattgatgcc ttagccgaag ccggtgccga
      421 tattattgcg gtggacggca cattccgcac ccgcccggtc agcattgaag aggcggtaaa
      481 acgcattcac gaaaaaggtt gcttggcaat ggcggattgc tccacccttg aagaaggttt
      541 acattgccaa caattaggct ttgatattgt gggtagtaca atgtctggct acaccggtgg
      601 cgaaatgcct gatgagccgg actatcaatt agtgaaagat ttaaaagctg ccggctgtaa
      661 tgttatggcg gaaggtcgct acaacacgcc tgaactggca aaaacggcga ttgaaatcgg
      721 ggcggattac gttaccgttg gatcggcatt gacccgctta gagcatattg tgagctggtt
      781 tgccgactct gtaaaatcgg ctcaaaaata ggagctgctt atgcgttgtt tagcaataga
      841 catcggtggc acgaaaattg ccacggcaat tgtgcaacac aatcaagtgt cacaacgcca
      901 acaaattcag accccgactg ataaaccaca agctgatcag gcaggcagtt tgcatcaagc
      961 gattggtgag ataatgcaac attatcaagg gcaatttgat tttgtggcag ttgcctcaac
     1021 aggcattatc aataaagggg tgcttaccgc attaaatcct aaaaatttag gcggattggc
     1081 agaatttccg ttgaaacaaa gcattgaaaa acataccgat aagccgattg cattattaaa
     1141 tgacgtgcaa gcggcggtgt gtgccgaata tttaaatgaa gaccaacaag cggtcaaaaa
     1201 ttttgtattt attaccgttt ccaccggcgt aggcggcggc attattttaa acggcgaact
     1261 gcaaatcggc agtaacggca ttgcaggaca tatcgggcat actctagccg atccgaacgg
     1321 gcctctctgt ggttgtggac gagtcggctg tgtcgaagca gtcgcagcag ggcgtgcaat
     1381 agaggcagtt tccagccaat gggaaaagcc ttgttcaccg aaagaggtgt tcgagcgatt
     1441 ccgccaaggc aacccacaag cggtcgaatt agtacaaaaa tctgcaaaag cgattgctaa
     1501 tttaattgca gatttaaaaa tcagtttaga tatacaaaaa gtggtgttag gtggcagtgt
     1561 gggcttagcg gaagggtatt taccgcaggt gtccaactat ttagctgaaa tgccgagcgt
     1621 atatcattgc gtattggaaa atgctctatc cgggcaagat gccggcttaa tcggtgcggc
     1681 ttggtgggcg gaaaaccaac ttaatcaagg ttttactctt taacaaattc taactccgga
     1741 gatccaaatg aaaaacttaa aaggtatttt tagtgcgtta ctcgtctctt tcaacgaaga
     1801 cggcaccatc aacgaaaaag gcttacgcca aattatccgc cacaacatcg acaaaatgaa
     1861 agtggacggt ttatatgtcg gcggttcaac cggtgaaaac tttatgcttt ctaccgaaga
     1921 gaaaaaagaa attttccgca ttgccaaaga cgaagcgaaa gatgaaatcg cccttatcgc
     1981 ccaagtcggt agcgtaaact taaaagaggc ggtagaactg ggtaaatatg ccacagaatt
     2041 aggctacgac agcttatctg cggtcacacc gttctactac aaattcagct tcccggaaat
     2101 caaaaatttc tacgaaacca ttattcgtga aaccggcaac aaaatgattg tttactccat
     2161 tccgttctta accggcgtaa acatcggtgt gagccaattc gctgaattat ttgaaaacga
     2221 aaaaatcatc ggtgtaaaat tcactgccgg cgatttctac ctgttagagc gtttacgcaa
     2281 agcgttcccg aaccacttaa tctacgcagg tttcgatgaa atgatgctac cggcaacggt
     2341 attaggtgtg gacggtgcaa tcggctcaac cttcaacgta aacggtattc gtgcaagaca
     2401 aatctttgaa ttagcccaac aaggcaaaat caaagaagca tttgaaatcc aaaatgtaac
     2461 caacgattta atcgaaggca ttttaggcaa cggtttatac caaaccatca aaggcttatt
     2521 ggagttagaa ggtgtgcaag caggttactg ccgtgagccg atgacgaaag aacaaactcc
     2581 ggcacaaaaa gaagtgacca aacagttaaa agctaaattc ttgtaattta agttgcaaaa
     2641 ggctaaacga aaatcaccgc ttgtagtaac aagcggtgat tttttctcaa cgacttgcaa
     2701 ttaatgaagc cattctgctt tttctaccac ggtgtaacgt gtcaccatta tattttaatc
     2761 gcactttttc ttggcgagta ttttctgatt tcaactctct acctatatca taaaaccctg
     2821 tactccagat attttgagta atatccgctt ttacaataat atcattgaag ccgttggtct
     2881 gtttgttatc catttgcaat accaagtttg tgtttccgtt ttggtttctt tatcatctaa
     2941 aatagaccaa aaagtcatcg ggtaatcaaa aacaagtttt aacttttctt tttccggcac
     3001 aaataacatc aaatgatcat ttgcagaata ctcaggagca cttggaccac gagcagcatt
     3061 attaaacctc acgccgaaag ctcttacttt gtcgtttaga atataacggg cggtatcaat
     3121 tttaagacta tctgcattca catcgataga ggcatcgatc tccatatcct gctcccacga
     3181 ttgcaatatc gcattatcat tcacacgagc aacaaaaagc gtccacttag gtgtgttttc
     3241 gtcgttttga taattttctt tgctgtattt aggctctatt accgcataac tcactaaggt
     3301 ttccgttgaa ttgttaggag ctgttttaca ggcagaagct aaagtacgag cagaaagttt
     3361 ctcttgagaa atcttaaagt aatgagcaat aatttgatgt tgttgctcag cacaaacctg
     3421 tgtgttatta ccttcatctg cttgagttgc aagcgtaagt aaaagaacta agaggagtat
     3481 ctttttcata tagatttatc cgtatagaaa gataactcat tctatggggt actgactctt
     3541 tttgcataga gagaaagaaa aaaatttggt cggaaaaata acggcgagcc tagcgaaaac
     3601 aagggttcgc cgtaatttta tgggggaagc tatgtaaaaa acaaaatgag caggattaga
     3661 aaaaaatgag cagtttttgc gatatttttt gcataggttt gatcggtttt aaaactattt
     3721 taaatctgtt ttaaactaaa gagtgtccga tgtggactac ctgcccgatg atttggaggt
     3781 tttgagcttc ttcggcggta atctgtattg ggcggtagga ctcgttatcg ctaattaatt
     3841 caatatagtc ccattctttt ctaatccgtt taacccatac gctttcccca ttctgcacaa
     3901 gataaatctt atcatcacca cgaggctctc gtttgctgaa atctaccagc atttggtcac
     3961 tgtctgcaat ggtagggtgc attgatgttc catttgccca aaacacgccg cagttgtggg
     4021 cttttacgcc gaggctttgc agcaggctgt cggcgtaagg ttcttgcccg tctggcttcg
     4081 ttacgccctc gttaaagctg ccaaagcctg ctgaaacgtt gattgttctg aagctgttaa
     4141 tcattgaaac aatatcatca gccatttttt ctagtcgctc aaactctctt gccacaatac
     4201 tggtattctc tgattgtggt ttgctttcct tacctgttgc cagccattca aggctcacat
     4261 ttgacacctc tgctattttt acaagatttg aacgagatgg atctgcctct ccttttaccc
     4321 acctagatag tgatgcttgg gctaccccca ctgctcttgc aaactcactg taattgtcct
     4381 taaagctatt ttttgcaatc catttcattc tttctgagaa gctctcatcg taaatgtttg
     4441 gtttactcat cttttacgtt tccattaata aagttttact cgtaaaacat actcataaac
     4501 cattgatttt taagttattt atgaataaag caaaaatatt ttagatgttt ttatgtttta
     4561 cggttgattt gtttaaagtt ttacggttaa atacacgcat caaatagata aaaagaggtg
     4621 tatcgaatga gtgtattaaa cgatgttaaa aaaaccgcac agagagattg gcataaagcc
     4681 gatattcttg cggaacttcg taaaaaaggt tggtcacttc gttctttggc taaagcaggc
     4741 aatgttagcc ataacacgct gaagtctgtt ttagacaagc cttaccccaa aatggaacgc
     4801 ttagttgcaa atgccatagg ggttgcccct gaagtgattt gggctgggcg aacagcagaa
     4861 cgcattgaac gtaatcggcg accaacttta actaataagt tttaatcata aatgatcttt
     4921 aacgtaaaag atataggaga agaacattta tgagcgaatt aagtttaaaa acgcactatt
     4981 cagtggctga attgttaagt tttaagttat ctagcctacc aagtgctcat aaaaatgtgc
     5041 ttgagaaagc aataagggaa aactggcaat ctcaaaaacg caaaggcaga ggcggcgggg
     5101 ttgaatatga attaatcagt ttacccaccg aagtccaaca ggaaatccgc accaagttac
     5161 tcaaacttct tcctgctgaa atcagcaaag gcgaattgag tgtagtgcga caaaacattg
     5221 atttggagca gataaccgat aaacaacttt ctacggcaga tgctcgcatt atggtggtgc
     5281 ggtggttctt gatgcaggaa gtgcagttgg ggctaagccg cacaaaaacg ctagaccaag
     5341 tcattgcagc ggtggcaagc agtgaaattc ctgctgaaat ttgcaaagcg attatggcgg
     5401 gcaatagtaa agccggtgga aagttaaagc tatctaaacg caccttgcat agttgggtgc
     5461 tggcgtatga ggcaggcgaa aattcagcgg aacggttaaa acagatgata ccgctgaaaa
     5521 cacaaaaacg tgccgtgccc gagcggtgtg gttggttgca agcctttctg ccgttttatc
     5581 aaacctttag caatgtggca ctcacacagg cttatgctca atttgcaatg cagtatgagg
     5641 gcgaagattt acctacagag agccaagttc ggtatgcctt gaagcagttg cccgattatg
     5701 tggtgcaaca agggcggaaa ttgccggtga agaagatccg gctcgcccgt taaataccct
     5761 tgaagttaaa ggcttaacgg tcgtagatga ctcgcagaaa ccgttattta gcgaagtaaa
     5821 ccaagcgtta tatcacggtt taagcccgat tgaggtggtg gcaagccgtg tgcagattac
     5881 ccgtgcgatt actacataca ccaaaaacgt gacgaatact gatgacccaa gctatttgga
     5941 tttaaccacc attcgtacgc ttgactatgt gcgtaaggca atccaaaccc gtcagcgttt
     6001 gcgtttccct cgtgcgaaaa actcccaccg tattgtggca aaagtacgct cggagatttt
     6061 agatgtgctt tatcagcttg agggcgaaga ggtaatcgaa aatgtggaag cgtggaaaaa
     6121 tcgcttatta gtggtgcgta atcaagaccc gacttacttg gatttagaaa tccctgcgga
     6181 tgtggttaat ggcttgcacg ttattcgtaa caaaattacg ttaattttat agggggtgct
     6241 agatgtcaat tgtttatgaa ggctcttgcg tgcttgaggt agatggcgtt gagattgaaa
     6301 ttaccaaact tgatgtaaaa accgtaaccg gtcgcaagct ggttaaaacg atgaacagtt
     6361 ccggacgtgc aagaggttat tcgcaaggca ttgcggaata tactctctcg gtgactgcag
     6421 ttgagcctca agacggttcg gtaattgatt gggataacat taaaaatgcg aagctcacta
     6481 aatacccgtt agacaatagc gaaaaacgca cctcttattt aggctgtttt actaccgatg
     6541 tgagcgagtc ttacaccgtt gatggtgagt cggtgcgtga tattcaactc aacgcattgc
     6601 gtaaggtgaa tgaataatga aaatcacttt aaatcttggc attgaatata acgggaaaat
     6661 tcgccgtgat tgtacggtgc gattgctgac gatgggcggt caatgcacgg ctcaagaaat
     6721 gattgcggat ttagggtttg aggaaaactc ggaagcccta tcaaagcgtg aacaaacctt
     6781 ggtcgatatg gcttaccttt cgcagcaagt tacctttgat ggtatttatc aaggtaatgt
     6841 gacggctcaa tttttatttg acaatttagc taccgaagac tactggcagt tgattgaggc
     6901 aacgctcaca ctccgaaaaa agcggttaga aaatggggtg agccaaagcg agagcgaaag
     6961 cccggcaaat cccgatttgg cgtagcagaa gcctttaagc agtaccgaca agcggtgatt
     7021 ttactctcaa aatttgcaat taccgccgag caggtatggc aaatgccgtt gcttgaggta
     7081 tttgcgtgga ttgagagcta cttggagagt gagggcgtaa aaacaccgca tagcccaatg
     7141 ggtgcgaata gcgaagtgaa acgcgaaagc tatgtcttta ctcggtgtgg caaaattgaa
     7201 taggggcgaa agcccctttt ttgtacttta aaatgagttt aaagagactt taaaaatggc
     7261 aaaaaatatg aaagtgcaac tggaattgac ggcaaaagat actgcctccc cagttttaaa
     7321 ccaagccgct aaaaccgccg aaaaagcctt taaaaataca gagcgagcgg cacaaaaaag
     7381 tggcaatgct caaacgcaag cagcacagaa agcagcgaat gctacagaaa gctcaaatcg
     7441 gaaaatagaa cagagttacc gtaatgcccg ccgagtggct gccgatgtga gccgagcccg
     7501 tgaaacatta ggtgttcg
//
LOCUS       DANLXS010000112         7498 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.112, whole genome shotgun sequence.
ACCESSION   DANLXS010000112 DANLXS010000000
VERSION     DANLXS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.112"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            1..170
                     /locus_tag="PXD71_001799"
                     /pseudo
     CDS             1..170
                     /locus_tag="PXD71_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            422..1612
                     /locus_tag="PXD71_001800"
     CDS             422..1612
                     /locus_tag="PXD71_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL6291895.1"
                     /translation="MSTSQQLIQQTEHFGANNYLPLPIVISKAKGIWVEDPEGNRYLD
                     MLSAYSAVNQGHCHPKIVQALKDQADKVTLTSRAFYNDQLGIWYEKICKLANKEMALP
                     MNTGAEAVETAIKTARRWGYDVKGIAENQAEIIACVGNFHGRTMAAVSLSSDPDYKRG
                     FGPMLGGIKLVPYGDLEALKAAITPNTAAFLVEPIQGESGILIPPEGFLEQAYELCKE
                     NNVLFIADEIQAGLGRTGKLFATEWEGFEPDIYILGKALGGGVFPISCVLANRDILGV
                     FNPGSHGSTFGGNPLACAVSIASIDVLLEEKLAERSRELGEYFLAELKKIQNPIIKEV
                     RGRGLFIGLELHQPARPFCEKLKEKGLLCKETHDTVIRFAPPLVIEKFNLDWALEKIR
                     EVLA"
     gene            1687..3198
                     /gene="nhaC"
                     /locus_tag="PXD71_001801"
     CDS             1687..3198
                     /gene="nhaC"
                     /locus_tag="PXD71_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL6291896.1"
                     /translation="MLILQTFSKNQPLVKGGFMSNRTEITDQNIRIPFRYAILPVIIM
                     VSIMAVAIIKFEASPHVPLLIGAISAAVIAMAHGYRWETIEKGIYRGIRMALPAIVII
                     IMIGLTIGAWLGGGIVATMIYYGLKLISPSLFLVTISIICAIVTLAIGSSWSTMGTIG
                     VAAMGIGFSLGIPAPMVAGAVISGAYFGDKMSPLSDTTNLAAGITETNLFEHIRHMFI
                     TTVPAFLIALSVYFYFGMEFRAENLDLAKIEDIMQAMQEHFLISPWLLLVPLLVVVLV
                     AKKVPALPALAAGIILGWLCHVFVQGGDVSVAVITLHDGYKIESGHEMIDKLFNRGGI
                     ESMMYTISLTIVAMTFGGIAEQAGMLHSVVEKILTLAKSAGSLISATIVSAFITNVTT
                     SEQYISILLPGRMYIKAYRDKGLSSRNLSRALEDGGTVTSVLVPWNTCGVYAFSMLQV
                     SAFEYAPYAIMNWLIPLIAIGLATLNIKIERLPPEELAKEPEEITLPKRGCCP"
     gene            3315..6914
                     /gene="putA"
                     /locus_tag="PXD71_001802"
     CDS             3315..6914
                     /gene="putA"
                     /locus_tag="PXD71_001802"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HDL6291897.1"
                     /translation="MMSYQLLPNTTIRPELSRHYRFDEQTLVQNLIEQAHCDEQADNI
                     KKLTTQLVGKVRTARQKASGVDALMHEFSLSSQEGVALMCLAEALLRIPDTATADKLI
                     RDKIAKGDWRSHTGNSPSLFVNAAAWGLVITGKLVSTHSESSLSSALSRLIAKGGEPL
                     IRKGVDVAMRMLGKKFVTGETIQQAIKNGEKRFAMGYRYSYDMLGEAALTAEDAKRYY
                     NDYVASIHAVGATSKGLGVYKSSGVSVKLSAIHPRYSLSQHQRVMDELYPRLKELFLL
                     AKKYDIGLNIDAEEADRLELSLDLMDRLLADPDLAGFNGIGFVVQAYQKRCPYVIEHL
                     IETARKHNRQLMIRLVKGAYWDTEIKRAQMDGAEGYPVFSRKVHTDLNYIVCAKKLLA
                     AQDVIYPQFATHNAQTLATIYHLAQGKRFEFQCLHGMGESLYDQVVGSDNLNVQCRIY
                     APVGTYQTLLAYLVRRLLENGANSSFVSQIVDENLSVDDLAQAPVQKAELTNGTMHPK
                     IPLPVDLFDDNRVNSKGYDLTDAVSLQNLENALNELTKQHYRAEPQLANDNTSGQESR
                     KIYNPANQAELVGEVIDATENDMEQAFESAAAFQSEWANTSPAVRAECLEKMADLMEA
                     NMPQLFDLAVREAGKTLNNAIAEVREAVDFCRYYAKEVRNNIEAYKNPRGIVVAISPW
                     NFPLAIFVGEVSSALVAGNVVLAKPAEQTSLMAHYAVSLFYQAGVPKAALQCLPGRGK
                     TVGVALVADHRVNGVIFTGSTETAKNINRNLHKNEKIIPLIAETGGQNAMIVDSSALG
                     EQVVIDVLNSAFDSAGQRCSALRVLYLQRDVADHILTMLKGAMAELKVGLPQHLTTDV
                     GPVIDKVAQQRLLDHIENLKKSAKSYYQVAISSEIAENGIFVPPTLLEIDHISQLKHE
                     VFGPVLHVIRFDADKLDQVIADINSTGFGLTSGLHSRIDETVDLWLKSVHAGNLYVNR
                     NTVGAVVGVQPFGGMGLSGTGPKAGGPLYLQRLVNRQEWTLNGLQGNAQAVDFDLNFA
                     IRKVLSGAEQQTALDLAKSLARRSPLGKTYPMQGITGEKNEMIFVARPQIGISKGNLA
                     SQVQALIAIVTVGSQAVAEKSSELAKHASAFGKKLQVVDDFNSLDHLSTLIVLDLLSS
                     EEKLHHAERKGAILTYIEGLDLALSLLPLVHEKAISINTAAAGGNASLMSEMD"
     gene            7023..>7498
                     /locus_tag="PXD71_001803"
     CDS             7023..>7498
                     /locus_tag="PXD71_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha3 family transposase"
                     /protein_id="HDL6291898.1"
                     /translation="MRKSRLSQYKQHKLIELFVAGVIARTAAELVNVNKTTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEIDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQSATLLPIIREKVKPDSLVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNH"
BASE COUNT         2146 a         1553 c         1764 g         2035 t
ORIGIN      
        1 gaattgagaa tttttggagc caagcaaaac gccatttgcg taaatttaac ggcataccta
       61 aagctcattt tgagctgtat ttgaaggaat gcgaatagcg ttttaatcac agtgacttaa
      121 aatctcaaat ttccatttta aaacaattgg ttaggggttg tttaagttag ttatctagga
      181 cagccctaaa ttttgtagtt ggaatttcaa cggagaaagt tatctataaa cttacaaaag
      241 ttatagaaaa tggaggcatt tttgcttaaa gtatgctatc tattcatatt aattgcatag
      301 ttaatcaaca actgaagaaa aattagaagt agatcataaa atcaacaaaa agtccgtgct
      361 ttttagctaa agattgttaa actcttttgt gttacaagtt tttaacataa aggagttttt
      421 tatgtctact tctcaacaac tcatccaaca aaccgaacat ttcggtgcca ataactacct
      481 acctctacct attgttatta gtaaagctaa aggcatttgg gttgaagatc ctgagggtaa
      541 tcgctatttg gatatgctta gtgcctactc agcggttaat caagggcatt gtcatccaaa
      601 aattgtacag gcactaaaag atcaagcaga caaagtaacg ctgacctctc gagcatttta
      661 taacgatcaa cttggtattt ggtatgaaaa aatctgcaaa ctcgctaata aagagatggc
      721 attaccgatg aatacgggtg cagaagcggt agaaacagcg attaaaactg ctcgccgttg
      781 gggttatgat gtgaaaggaa ttgcagaaaa tcaagcagaa atcatcgctt gtgttggcaa
      841 ttttcacgga cgaacaatgg ctgcagtatc tctctcgtct gatccggatt ataaacgtgg
      901 tttcgggccg atgttaggtg gcatcaaact tgttccatac ggtgatttag aagcattaaa
      961 agctgcgatt acaccaaaca cagcagcgtt tttggttgag ccaatccaag gtgaatctgg
     1021 cattcttatt ccacctgaag gtttcttgga acaggcttat gaactatgca aagagaataa
     1081 tgtgctgttt attgctgatg aaattcaggc aggtcttggg cgtactggca agttatttgc
     1141 aacagaatgg gaaggctttg agccagatat ttatattcta ggtaaagcac ttggtggcgg
     1201 ggtgttccca atctcttgtg tgttagcaaa tcgtgatatt ttaggcgtat tcaatcctgg
     1261 ctcgcacggt tcgacatttg gtggtaatcc attagcctgt gcggtatcta ttgcctcaat
     1321 tgatgtactg ttggaagaaa aacttgcaga gcgttcccgt gagttaggtg aatacttctt
     1381 ggcagagctg aaaaaaatcc aaaatccaat tattaaagaa gttcgtggac gtggactgtt
     1441 tattggcttg gagttacatc aaccagcacg cccattctgc gaaaaactca aagaaaaagg
     1501 cttactctgc aaagagaccc acgatacagt tatccgcttt gctccccctt tggtgattga
     1561 aaaattcaat ttggattggg cattggagaa aattcgggag gtgttggctt aattgtcatt
     1621 ttctctatat tttatcctgc caaatcgcct ttaacccttt ttggcaggaa gcaaaacagt
     1681 tgtaaaatgc tgattttgca aactttttcc aaaaatcaac cgcttgtaaa aggtggcttt
     1741 atgtcaaaca gaacagaaat aacagaccaa aatatccgaa tcccgtttcg ctatgctata
     1801 ttgcctgtga ttattatggt atcgattatg gcggtggcta tcatcaaatt tgaagcctca
     1861 cctcacgttc cgttgcttat tggggcaatt agtgccgctg tgattgcaat ggcacatggc
     1921 taccgttggg agacaattga aaaaggaatt tatagaggca ttcgtatggc tttaccagcg
     1981 attgttatca ttattatgat tgggctaacc atcggagctt ggctaggtgg tggaattgta
     2041 gctacgatga tttactacgg cttgaaattg atctctccgt cgctgttttt agtaactatc
     2101 tcaataattt gtgctattgt gacccttgcg attggtagct cgtggtctac aatgggaaca
     2161 atcggtgtag cggcgatggg gatcgggttt agtctcggca ttccggcacc gatggttgcc
     2221 ggtgcggtga tctcaggagc atatttcggc gataaaatgt cgccactgtc agacactacc
     2281 aatctggcag cagggattac cgaaaccaat ctgtttgaac atatccgcca tatgtttatc
     2341 actaccgtgc cggcgttcct gattgcgtta tctgtctatt tctattttgg tatggaattt
     2401 agagccgaaa atctcgatct tgctaaaatt gaagacatta tgcaggcaat gcaggaacat
     2461 ttcttgattt cgccttggtt gctattagtg cctttattag tcgtggtgtt ggtggcaaaa
     2521 aaagtgcctg ccttaccggc attagctgca gggataattc ttggttggct ctgccacgtt
     2581 tttgtgcaag gcggtgatgt cagcgtagca gtgattactc tgcacgacgg ctataaaatt
     2641 gagagcggcc acgagatgat tgataagctg ttcaaccgtg gtgggatcga gtcgatgatg
     2701 tacaccattt cccttacgat tgttgcgatg acttttggtg gcattgcaga gcaagctgga
     2761 atgttacatt cagtagtgga aaaaatcctt acccttgcaa aatcagcagg tagcttaatt
     2821 agtgctacga ttgtatcggc atttattacc aacgtaacga catctgagca atatatttct
     2881 attttattac caggcagaat gtatataaaa gcctataggg ataagggact aagttcgaga
     2941 aacctttctc gtgcattgga agacggagga acagttactt cggttcttgt accttggaat
     3001 acctgtggtg tttatgcgtt ctcaatgttg caagtgagtg cttttgaata tgcaccgtat
     3061 gcaataatga attggctcat tccactgatt gcgattggct tggcaacctt gaatatcaaa
     3121 attgagcgat tgccaccgga agaattagcc aaagaacctg aggaaattac tctgccaaaa
     3181 cgtggttgtt gtccataatt tatctaaata gtatttaata acaataggtt aagttaaata
     3241 acaagcggtc ggttttatct taaaatttgc aagtttatac ataaatttga tcgcttgctg
     3301 tatagaaagg aaaaatgatg tcataccaac ttttacctaa caccactatc cgtcctgaat
     3361 tgagccgtca ttaccgcttt gatgagcaaa ccctggtgca aaatctgata gaacaagcac
     3421 attgtgatga acaggctgat aacattaaaa agctcactac ccaattagtc ggtaaagtcc
     3481 gaactgcacg ccagaaagca agcggggttg atgcgttaat gcacgaattt tcactctcca
     3541 gccaagaggg ggtggcgttg atgtgccttg ccgaagcatt actacgcatt cctgacaccg
     3601 ccactgcgga taaattaatt cgagacaaaa ttgccaaagg ggattggcgt tcacacaccg
     3661 gcaacagccc ctctctgttt gtgaatgcag cggcgtgggg cttggtgatt accggcaaat
     3721 tggtttcaac ccacagcgaa agctcactct catcagcact ttcacgcctg atcgccaaag
     3781 gcggagaacc gctgatccgc aaaggtgtgg acgtagcaat gcgtatgctc ggcaagaaat
     3841 ttgtaacggg cgaaaccatt caacaggcga ttaaaaacgg cgaaaaacgc ttcgcaatgg
     3901 gttatcgtta cagctacgat atgctgggcg aagcggcact caccgccgaa gatgccaaac
     3961 gctattacaa cgactatgtt gcctctattc acgctgtggg tgccacctcg aaaggcttgg
     4021 gcgtgtataa atcctcgggt gtgtcggtca aactctccgc cattcaccct cgttattccc
     4081 tttcccaaca ccaacgtgtg atggacgaac tctatccacg tctgaaagaa ctgttcttgc
     4141 tggcgaaaaa gtatgacatc ggcttgaaca ttgatgccga agaagccgac cgcctcgaat
     4201 tgtcgcttga tttgatggac agactgcttg ccgaccctga tttggcaggg tttaacggca
     4261 ttggttttgt ggtgcaggcg taccaaaaac gttgcccgta tgtgatcgag catttaattg
     4321 agaccgcccg taaacataat cgccagctaa tgattcgctt ggtaaaaggc gcatattggg
     4381 acactgaaat caagcgggcg caaatggacg gtgcagaggg ctatccggtc ttctcccgta
     4441 aggtgcatac cgatttgaac tacatcgttt gtgccaaaaa attgctggcg gcgcaagatg
     4501 tgatctatcc gcaatttgcc acccacaacg ctcaaactct cgccaccatt taccacttgg
     4561 cacagggcaa acgttttgag tttcaatgct tgcacggtat gggagagagc ttgtacgacc
     4621 aagtggttgg cagcgataat cttaacgtgc aatgtcgcat ttatgcccct gtcggtactt
     4681 atcaaaccct gcttgcctat ttagtacgtc gcttgttgga aaacggagcg aacagctcat
     4741 ttgttagcca aattgtggat gaaaatctca gtgtagatga tctggctcaa gcccctgttc
     4801 aaaaagcaga actgaccaat ggcactatgc acccgaaaat tccattgcca gtcgatttat
     4861 ttgacgacaa tcgtgttaat tccaaaggct atgatttaac tgatgcagta agcctacaaa
     4921 atttagaaaa tgccctcaat gaacttacca aacagcatta ccgtgctgaa ccacagctgg
     4981 caaatgataa tacaagcggt caagaaagcc gaaaaattta caatccagcg aaccaagctg
     5041 aattagtcgg cgaagtgatt gatgccaccg aaaacgatat ggaacaggcg tttgaaagtg
     5101 cggcggcgtt ccaaagtgaa tgggcgaata cttcccctgc ggtgcgtgcc gagtgcttag
     5161 aaaaaatggc ggacttgatg gaagccaata tgccacaact gttcgatttg gcggtgcgtg
     5221 aggcaggcaa aacgctcaac aatgcaattg ctgaagtgcg tgaagccgtc gatttctgtc
     5281 gttattatgc caaagaggta cgcaacaaca ttgaagcata caaaaatccg cgtggcattg
     5341 tagtagcaat tagcccgtgg aactttccgt tagcgatttt tgtgggtgaa gtttcatctg
     5401 cgttggtggc aggcaatgtg gtactagcaa aaccagctga acaaacttcg ttaatggcac
     5461 attatgctgt cagccttttc taccaagcag gtgtgccgaa agcggcgttg caatgcttac
     5521 cgggtagagg aaaaacggtt ggggtggcgt tggtggcaga tcatcgtgtg aacggcgtga
     5581 tttttactgg ttcaacagag acagctaaaa acattaaccg caatttgcac aaaaacgaga
     5641 aaattatacc gcttattgcc gaaactggcg gacaaaatgc aatgattgtt gatagctcgg
     5701 cactgggcga gcaggttgtg atagatgttt taaactccgc atttgactca gcagggcaac
     5761 gttgttcggc attgcgtgtg ctgtatctgc aacgtgatgt tgccgatcac attctcacaa
     5821 tgctcaaagg agcaatggcg gagttgaaag ttggtcttcc acaacactta actaccgatg
     5881 tcggaccagt aatcgataaa gtcgcacaac aacgcttgct cgatcatatt gaaaatctca
     5941 aaaaatcggc gaaaagttac tatcaggtgg ctatctcctc tgaaattgcc gaaaatggta
     6001 ttttcgtacc gccaacgctg ttagaaatag atcatatcag ccagcttaaa cacgaagtat
     6061 tcggaccggt gctacacgtt atccgttttg atgcggataa actcgaccaa gtgattgccg
     6121 acataaactc cacaggtttc ggtctaacca gcggtttgca tagccgaatt gatgaaacgg
     6181 tggatttatg gttgaaatct gtccacgctg gaaatctgta tgtaaaccgt aacacggtag
     6241 gggctgtggt cggcgtacag ccattcggtg gaatggggtt atcaggaact gggccgaaag
     6301 ctggcgggcc gctctatcta caacgtttag taaaccgcca agaatggaca ttgaacggtt
     6361 tacagggcaa tgcacaagcg gtcgattttg acttaaattt tgcaatccgc aaggttctaa
     6421 gtggagctga gcagcaaacg gcgttagatc tggccaaaag ccttgcacgc cgctcgccac
     6481 tcggaaaaac ctacccaatg cagggtatta ccggcgagaa aaacgagatg attttcgtgg
     6541 ctcgcccaca gattgggatt agcaaaggta atttggcttc acaggtgcaa gccttgattg
     6601 ccattgtaac ggtcggcagc caagcggtgg cagaaaaatc aagcgagctg gcaaaacacg
     6661 cctctgcctt tggtaagaaa ttacaggtgg tggatgattt caacagtctc gatcacctca
     6721 gcacactcat tgtgcttgac ctgctttcta gtgaggaaaa attgcatcat gccgaacgca
     6781 aaggggcgat tttgacctac atcgaagggc tggatttagc gctctcactg ctgccgttgg
     6841 tacacgagaa agcaatcagc atcaacaccg ctgccgcagg gggaaatgcc agcttaatga
     6901 gtgaaatgga ttaagcaaca aacaaaagag tataagaggg cagatcacaa tgatctgcct
     6961 ttttatttta aggggctgtc ctagataacg actaaaactt ccatttaggt taagataacc
     7021 ctatgagaaa aagtcgtcta agtcagtaca aacaacataa actcatagag ctgtttgtag
     7081 caggcgtaat cgctcgaaca gccgcagagt tagtgaatgt aaataaaacc accgcagcct
     7141 attattttca ccgtttgcga ttgctcatct atcaacatag ccctcatttg gaaatgtttg
     7201 agggagaaat tgagattgat gagagctatt tcggcggtca tcgcaagggt aaacgaggtc
     7261 gtggtgcagc aggcaaaacc gccgtattcg ggcttttaaa gcgtgacggc aaggtttata
     7321 ccgtagtagt gccgaacaca caatctgcta cacttttgcc gattattcgg gaaaaggtga
     7381 agcccgatag ccttgtttac accgattttt atcgaagcta tgatgtgctt gatgtgagtg
     7441 aatttaacca ttttcgtatc aatcacagca cacacttcgc agaaaatcaa aatcacat
//
LOCUS       DANLXS010000113         7449 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.113, whole genome shotgun sequence.
ACCESSION   DANLXS010000113 DANLXS010000000
VERSION     DANLXS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.113"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..491
                     /locus_tag="PXD71_001804"
     CDS             <1..491
                     /locus_tag="PXD71_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL6291899.1"
                     /translation="NEIKEEIYPIFEPIIGTIRLKTYFSYYAVFKDGLMIALYQNGAT
                     YLRIAKKDIEYIRQYPKTCILCDEKIGIQSRKFHYIPQVLIDDTPKFSSLLFGIIEEL
                     RTEKYKISQKRATQIRNLPNMNLKLERMLKKIGIHSIHDFTQEGYMSTFIKLILHRRL
                     TV"
     gene            779..2173
                     /gene="fumC"
                     /locus_tag="PXD71_001805"
     CDS             779..2173
                     /gene="fumC"
                     /locus_tag="PXD71_001805"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDL6291900.1"
                     /translation="MEFRIEKDTMGEVQVPADKYWAAQTERSRNNFKIGPEASMPAEI
                     IEAFGYLKKAAAYANHDLGVLAVEKRDLIAKACDEILANKLDDQFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTYPTAMHIAAYKKVVEHT
                     LPCVKRLQKTLAEKAEAFKDVVKIGRTHLMDATPLTLGQEFSAYAAQLAFGIKALENT
                     LPHLRELALGGTAVGTGLNTPQGYDVKVAEYIAKFTGLPFVTAQNKFEALATHDAIVE
                     THGALKQLAVSLYKIANDVRLLASGPRSGIGEILIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDVTISFAGTQGHFQLNVYKPVMAYNFLQSAQLLGDACVSFDEHCAVGIE
                     PNYPRIKQQLDQSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQF
                     DEWVRPEDMVGSLK"
     gene            2300..2410
                     /locus_tag="PXD71_001806"
     CDS             2300..2410
                     /locus_tag="PXD71_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL6291901.1"
                     /translation="MKKLILIALVTTLGLSACGVKGPLYFPEQAPKHEQK"
     gene            2515..3765
                     /gene="lysA"
                     /locus_tag="PXD71_001807"
     CDS             2515..3765
                     /gene="lysA"
                     /locus_tag="PXD71_001807"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL6291902.1"
                     /translation="MNHFEYKNNQLFAEEVAIADIIEQYGTPAYVYSRSTLERHWHAF
                     NNAFGSHPHLVCFAVKSNSNIALLNVMARLGSGFDIVSQGELERVLAAGGEASKVVFS
                     GVAKSHSEIKRALEVGIRCFNIESVSELHRINQVAGELGKIAPISLRVNPDVDAHTHP
                     YISTGLKENKFGVSVTQAREVYRLAKTLPNVKITGMDCHIGSQLTELQPFLDATDRLI
                     ILMEQLREDGIELHHLDLGGGLGVPYNGEEPPHPTEYAKALLEKLKGYENLEIIIEPG
                     RAITANAGILVTKVEYLKQNEDRNFAIVDTGMNDMIRPALYEAYMQITEVDKTLNRDK
                     AVYDVVGPICETSDFLGKQRELAISEGDLIAIRSAGAYGAAMSSTYNSRPQAAEIMVD
                     GNQAHLIKARATFADLWRLEKLLP"
     gene            3779..4408
                     /locus_tag="PXD71_001808"
     CDS             3779..4408
                     /locus_tag="PXD71_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HDL6291903.1"
                     /translation="MKLYGLTGACSFVPHVALEWVKLRANQDYAFQAVSREFIKSAEY
                     LALNPRGNVPLLVDGDLALTQNQAIVHYLDELYPEAKLFGSKTARDKAKAARWLAFFN
                     SDVHKSFVPLFRLPSYAEGNETLTKTIRQQSAEQILEQLAFANAHLENHIFFGEEISV
                     ADAYLYIMLNWCRLLGLDFSHLSQLSAFMQRVEADQGVDNVREQEGLKG"
     gene            4479..5924
                     /gene="tldD"
                     /locus_tag="PXD71_001809"
     CDS             4479..5924
                     /gene="tldD"
                     /locus_tag="PXD71_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDL6291904.1"
                     /translation="MLNQVSNSLLAPSGLELSHLTQVLDHFSGRHIDYADLYFQLSQD
                     ESWSLEDSIIKEGGFYIDRGFGVRAISGEKTGFAYADQITLKQLEQCAAAARSISPTS
                     GQLSVKNFKKTTACSRYPAINPLETISREQKVELLHLVDKTARMADPRVIQVNANLSA
                     VYEEMLVAATDGTLAADIRPLVRLSVSVLVEQNGKRERGSAGAGGRFGLDWFLAPSET
                     GFSRAESLTKEAVRQALVNLTAIPAPAGTMPIVLGAGWPGILLHEAVGHGLEGDFNRK
                     ESSLFSGKIGQMVTSELCTIVDDGTVPNMRGSITVDDEGVPSQRNVLIENGILKGYMQ
                     DKLNARLMGVAPTGNGRRESYAHLPMPRMTNTYLTEGNHEFEEMIESVEYGLYAPHFS
                     GGQVDITSGKFVFSTAEAYLIEKGKITKPVTGATLIGSGIEAMQQVSMVGKKMELDPG
                     IGTCGKEGQSVPVGVGQPTVKLDKITVGGRG"
     gene            5988..6869
                     /gene="prmA"
                     /locus_tag="PXD71_001810"
     CDS             5988..6869
                     /gene="prmA"
                     /locus_tag="PXD71_001810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL6291905.1"
                     /translation="MAWVQIRLNSTDKQAEEISDFLTEIGAVSVTFMDSQDTPIFEPL
                     PGETRLWGNTDVVGLFDAETDMNLIVEALKTSKLVDENFAHKIEQIEDKDWEREWMDN
                     FHPMQFGKRLWICPSWREVPDPNAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLEGK
                     TVIDFGCGSGILAIAALKLGAKQAIGIDIDPQAILASQNNAEANGVAEKLQLFLAKDQ
                     PRELQADVVVANILAGPLKELAPNIITLVKPNGNLGLSGILATQAESVCEAYSEAFTL
                     DPVVEKEEWCRITGRKI"
     gene            complement(7044..7119)
                     /locus_tag="PXD71_001811"
     tRNA            complement(7044..7119)
                     /locus_tag="PXD71_001811"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7084..7086),aa:Trp,seq:cca)
     gene            complement(7185..7261)
                     /locus_tag="PXD71_001812"
     tRNA            complement(7185..7261)
                     /locus_tag="PXD71_001812"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7225..7227),aa:Asp,seq:gtc)
     gene            complement(7272..7387)
                     /gene="rrf"
                     /locus_tag="PXD71_001813"
     rRNA            complement(7272..7387)
                     /gene="rrf"
                     /locus_tag="PXD71_001813"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2218 a         1661 c         1645 g         1925 t
ORIGIN      
        1 tcaatgaaat taaagaagaa atctacccta tatttgagcc tatcattgga acaattcggc
       61 taaaaaccta cttttcctac tacgcagttt ttaaggatgg attgatgatt gcattgtatc
      121 aaaatggagc aacttattta cgtattgcga aaaaagatat tgaatacatt cgtcagtatc
      181 ctaaaacctg tatcttatgc gatgaaaaaa ttggtataca aagtagaaaa tttcattata
      241 ttccgcaagt tctcatagat gatacaccaa aatttagctc gctactcttt ggaattatag
      301 aggaactccg aacggagaag tacaaaataa gccaaaagcg agctacacaa atccgaaacc
      361 taccaaatat gaatttaaag ttagaaagaa tgttgaaaaa aatagggatt cattccattc
      421 atgattttac tcaagaaggg tatatgtcaa cttttatcaa actgattctc caccgaagac
      481 ttactgttta agctaaacgg agctttaaat caccaatata tttacacctt cacagatcaa
      541 caaaaaaggg aactaatgca agaagcaaat caagcgctat ataattcagg cctaagaaaa
      601 agatttaacg ccctgtagtt aaataatgtg atccaaatca taaaatataa aaaaaccaat
      661 aaggaattgc aattcgcagt tcctttcttt ttcttttata tgctagtatc accttatacc
      721 tctaaattat tagtgcctac tgattttcaa ttaatccaac aatttaggag agcgcatcat
      781 ggaattccgt atcgaaaaag acacaatggg cgaagtgcaa gttcctgccg ataaatactg
      841 ggcggcgcaa accgagcgtt cacgcaataa cttcaaaatc gggccggagg catcaatgcc
      901 ggcggaaatt atcgaggcgt ttggctatct gaaaaaagcg gctgcctatg ctaatcacga
      961 tttaggcgta ctagcggtcg aaaaacggga tttaattgca aaagcctgtg atgaaatttt
     1021 ggcgaacaaa ctcgatgatc aattcccgct ggtgatttgg caaaccggct cgggtacgca
     1081 gtcaaatatg aacgtgaatg aagtcgtggc aaaccgtgca cacgtgttaa acggcggcaa
     1141 gctgggtgaa aaatcgatta ttcacccgaa tgatgatgtg aataaatccc aatcttctaa
     1201 cgacacctac ccaactgcaa tgcacatcgc tgcttacaaa aaagtggtgg aacacaccct
     1261 accttgtgtg aaacgtctgc aaaaaacctt agcggaaaaa gccgaagcct ttaaagatgt
     1321 ggtcaaaata gggcgtactc acttaatgga tgctaccccg ctcacgctcg gtcaagagtt
     1381 ctctgcctat gcagcacagt tagcattcgg catcaaagca ttggaaaaca ccctgccaca
     1441 cctgcgtgag ctggcactcg gcggcacagc agtcggcacc ggcttgaata ccccgcaagg
     1501 ttacgatgtg aaagtggcgg aatacattgc gaaattcacc ggtttaccgt tcgttaccgc
     1561 ccaaaacaaa tttgaggcat tagcgactca cgatgcgatt gtcgaaaccc acggtgcatt
     1621 aaaacagctt gcggtgtcgc tctacaaaat tgccaacgat gtccgtttat tagcctccgg
     1681 tcctcgttca ggcattggcg aaattctgat tccggaaaac gagccgggtt catcaattat
     1741 gccgggtaaa gtaaacccaa cccaatgcga agcaatgaca atggtatgtg cccaagtatt
     1801 aggcaacgat gtaaccatct cttttgccgg cacgcaaggg catttccaac tgaatgtcta
     1861 taaaccggtg atggcgtaca acttcctgca atcggctcaa ctgctaggcg atgcgtgcgt
     1921 gtcgtttgac gaacactgtg cagtcggcat tgaaccgaac taccctcgca ttaaacaaca
     1981 gctcgaccaa tcgctgatgt tagtgaccgc attaaatact cacattggtt atgaaaatgc
     2041 agcgaaaatc gccaaaactg cccacaaaaa cggcacaaca ctgaaagaag aggcgattaa
     2101 cttaggctta gtcacggcag aacagttcga tgaatgggtt cgtccggaag atatggttgg
     2161 cagcttgaaa taagcccaac ccttctcttg taatctgatt tacacctcaa tctaccggta
     2221 agattgaggt gtaatttttt atagcgaggg tactgatttt gttttagaat gaagcatcat
     2281 tttttaagcg gaaaaaccta tgaaaaaact cattttaatc gcattagtta ctactttagg
     2341 cttaagtgct tgtggggtca aaggcccact ctacttccct gagcaagccc ctaaacatga
     2401 acaaaaataa gcattgcaag cggtctgttt tggctaaaat tttgcaaatt tttgtgaaaa
     2461 aatgaccgct tgttgcctct tttctttcaa aatcccaaac acaacggact ttcaatgaac
     2521 cattttgaat ataaaaacaa ccaacttttt gctgaagaag tcgctattgc cgatattatc
     2581 gagcaatacg gcacgccagc ttatgtttac tccagatcta cccttgaacg ccactggcac
     2641 gcattcaata atgcgtttgg ctcgcaccca catttagttt gctttgcagt caaatccaac
     2701 tctaacattg ccttgttaaa tgtaatggct cgtctcggtt caggctttga tattgtctct
     2761 caaggcgagt tggagcgtgt cttagctgcc ggcggcgaag cctctaaagt tgtcttttcc
     2821 ggtgtggcaa aatcgcacag cgaaatcaaa cgagcattgg aagtcgggat tcgttgcttt
     2881 aacattgaat cggtttccga acttcaccgc attaaccaag ttgccggtga actaggcaaa
     2941 atcgcaccga tttcactgcg ggtaaacccg gatgtggacg cccacactca cccatacatt
     3001 tccaccggtt taaaagaaaa caaattcggc gtgagcgtta ctcaagcccg tgaagtgtat
     3061 cgcttagcca aaaccttacc gaatgtgaaa attaccggta tggattgtca catcggttcg
     3121 caattaaccg aattgcaacc ttttttagat gcaacggatc gcttaatcat cttaatggag
     3181 caactgcgtg aagacggcat tgagctacac cacttagact taggtggcgg tttaggcgtg
     3241 ccttacaacg gcgaagagcc tccgcatccg accgaatatg ccaaagcctt gctggaaaag
     3301 ctcaaaggct atgaaaactt agaaatcatt attgaaccgg ggcgtgccat taccgcaaat
     3361 gccggtattt tagtgacgaa ggtggaatac ttgaaacaaa atgaagatcg taattttgcg
     3421 attgtcgata ccggtatgaa cgatatgatt cgcccggctc tgtatgaagc ctatatgcaa
     3481 attacggaag tggataaaac cttaaaccgt gacaaagctg tttacgatgt ggtcggtccg
     3541 atttgcgaaa cctcggattt cttaggcaaa caaagagaat tggcaatttc tgaaggggat
     3601 ttaatcgcta ttcgttctgc cggtgcttat ggggcggcga tgtcttcaac ctacaactct
     3661 cgcccacaag cggcagaaat tatggttgat ggcaaccaag cccatttaat taaagcccga
     3721 gccacttttg ctgatttatg gcgtttagaa aaattattac cttaatgagg aagaaaagat
     3781 gaaattatat ggattaaccg gtgcttgttc atttgtacca cacgttgccc tagagtgggt
     3841 gaaattgcgt gccaatcaag attatgcgtt tcaagctgtt agccgtgaat ttattaaatc
     3901 ggcggaatat ctggctttaa accctcgtgg taacgtacca ttgttggttg atggcgactt
     3961 ggccctcacg caaaaccaag ctatcgtcca ctatttagac gagctttatc cggaagctaa
     4021 attattcggc agcaaaaccg ctcgtgataa agccaaagcg gctcgttggt tagctttttt
     4081 caattcagat gtgcataaat cattcgtgcc actgttccgc ttgccaagtt atgcggaagg
     4141 caatgaaacg ctcaccaaaa ctattcgcca acaatcggct gagcaaattt tagaacaact
     4201 ggcttttgcg aatgctcatt tagaaaatca tattttcttt ggcgaagaaa tttcggttgc
     4261 cgatgcgtat ctctacatta tgctcaactg gtgtcgccta ctagggttgg atttctctca
     4321 tctttcccaa ctttctgcct ttatgcaacg agtggaggca gaccaaggcg tagataatgt
     4381 gcgtgagcaa gaaggcttaa aaggttaatt caccattgtt catttaagac ggtagatttt
     4441 atgttaaaat ctaccgtctt ttatttttag gaatcaccat gctaaatcag gtttcaaaca
     4501 gcttgcttgc tccaagtggg cttgaattat cgcacttaac tcaagtgtta gatcacttct
     4561 ccggccgtca tattgattat gccgatctct acttccaact cagccaagac gaaagctgga
     4621 gcttggaaga cagcatcatc aaagaaggcg gtttttatat cgaccgaggg ttcggtgtgc
     4681 gagccatatc aggcgaaaaa accggctttg cctacgccga tcaaatcacg ttaaaacagc
     4741 ttgagcaatg tgccgctgcc gcacgttcaa tcagcccaac aagcggtcaa ttatcggtaa
     4801 aaaatttcaa aaaaacgacc gcttgttctc gctacccggc aatcaacccg ctagagacca
     4861 tcagccgtga acaaaaggtc gaattgctcc atttggtgga taaaaccgct cgaatggcag
     4921 atccgagagt gattcaagtg aatgcgaatc tctcagcggt atatgaagaa atgttagtgg
     4981 cagcgactga cggcacactg gctgcggata ttcgcccgtt agtgcgttta tctgtgtcgg
     5041 tactggtgga acaaaatggc aagcgtgaac gaggttcagc aggtgccggt ggtcgctttg
     5101 gtttagattg gttcttagca ccaagcgaaa ccggctttag ccgtgcggaa tcgcttacca
     5161 aagaagccgt tcgccaagcc ctcgttaatc tcacagccat tcctgcaccg gcaggcacga
     5221 tgccgattgt tctcggtgct ggctggccgg gcattttgct ccacgaagca gtcggacacg
     5281 gcttggaggg tgattttaac cgtaaggaat cttcgctctt cagtggtaaa atcgggcaaa
     5341 tggtcacctc tgagctttgc accattgttg atgacggtac ggtgccgaat atgcgaggct
     5401 caatcacggt agatgacgaa ggcgtgcctt cgcaacgtaa tgtgttaatt gaaaacggca
     5461 ttctaaaagg ctatatgcaa gacaaactca atgcccgcct gatgggagtt gccccaaccg
     5521 gcaacggcag acgggaatct tacgctcatt tgccgatgcc acgaatgacc aacacctacc
     5581 tcaccgaagg caatcacgaa tttgaagaga tgattgaatc ggtcgaatac ggtttatatg
     5641 ctcctcactt cagtggtgga caagtggata ttacctccgg caaatttgtg ttctccaccg
     5701 ccgaagccta cttaatcgaa aaaggcaaaa tcaccaaacc tgttaccggt gcaaccctga
     5761 tcggcagcgg catagaagca atgcaacaag tttcaatggt cggcaaaaaa atggaactcg
     5821 accccggcat cggcacttgt ggcaaagaag ggcaatccgt accagtcggc gtagggcagc
     5881 ctacagtgaa gttggataaa attacggtgg ggggaagagg gtaatctctc cccgtttgca
     5941 aaatataaag aaaatatcac cgcttgtagc gataaaagga aaacaaaatg gcttgggtac
     6001 aaattcgttt aaacagtaca gataaacagg ctgaggaaat cagcgatttt ttaaccgaga
     6061 ttggagcggt gtcagtgact tttatggata gtcaagatac cccaattttt gaacctcttc
     6121 cgggggaaac ccgtctttgg ggaaataccg atgtggttgg gcttttcgat gcggaaactg
     6181 atatgaatct tatcgtagaa gcactaaaaa ccagcaaatt ggtggacgaa aactttgctc
     6241 acaaaatcga gcaaatcgaa gacaaagact gggaacggga gtggatggat aatttccacc
     6301 caatgcagtt tggcaaacgg ttatggatct gcccaagctg gcgtgaagta ccagacccga
     6361 atgcagtgaa tgttatgtta gaccctggtt tagcatttgg caccggcaca cacccaacga
     6421 cctcgctttg cttacaatgg ttagatagct tagatttaga aggcaaaacg gttatcgact
     6481 tcggctgtgg ctccggtatt ttagctatcg ctgcacttaa actcggtgca aaacaagcca
     6541 tcgggattga tatcgaccca caggcgattt tagctagtca aaacaatgct gaggcgaacg
     6601 gcgtagcaga aaaactacaa ctattcttag ccaaagatca gccaagagag ttacaagccg
     6661 atgtcgttgt tgcgaatatt ctagccggcc cactcaaaga gttagcaccg aatatcatca
     6721 cgctggttaa accaaacggc aatttaggct tatccggcat tcttgctact caggctgaaa
     6781 gtgtgtgtga ggcttatagc gaagccttta cgctagaccc tgttgtagaa aaagaagaat
     6841 ggtgtcgtat tacaggccgc aaaatctaat ataagaagcc ctgttttaaa cagggcttac
     6901 tttttgataa attctactac tctttacctg tcttgggaat cgttctccct aaattaatta
     6961 acacaagcgg tagaaataac aggaaaattt acaaattaca taaatgaaaa aagccgactg
     7021 aaatcagtcg gctttctaaa taatggcagg ggcggagagg ctcgaactcc caacacccgg
     7081 ttttggagac cggtgctcta ccaattgaac tacgccccta ttgataggta ttcattttgc
     7141 acttataggg gcgaattact attcgcccct gcattaaaga ttattggcgg aacggacggg
     7201 actcgaaccc gcgaccccct gcgtgacagg caggtattct aaccagctga actaccgctc
     7261 ctaaattaat agattgataa tgccctactc tcacatggcg aatcaccaca ctaccatcgg
     7321 cgttactgcg ttttacttct gagttcggta tggagtcagg tagagccaca gcactctggt
     7381 tatcaatcaa attctttcat ttagtcagtt tactaaaata tagtttatac tttctaataa
     7441 actcactaa
//
LOCUS       DANLXS010000114         7406 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.114, whole genome shotgun sequence.
ACCESSION   DANLXS010000114 DANLXS010000000
VERSION     DANLXS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.114"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..507
                     /locus_tag="PXD71_001814"
     CDS             <1..507
                     /locus_tag="PXD71_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein"
                     /protein_id="HDL6291906.1"
                     /translation="LFIAQIKQNLSELNVFSTDNIFLNSPYFSQQTGLVSVFIAEIEK
                     TVELLLNQTEVLYSEFYAEKLVKQVDALKNAVEKIQSKPESAQFHSSYQFSPNIHRLA
                     PNKRLQEYRKALRALNEKISWLVEQNLNTQNEATKQTLQNQITETEYRKMKCLKAIED
                     LEQELLFK"
     gene            complement(511..584)
                     /locus_tag="PXD71_001815"
     tRNA            complement(511..584)
                     /locus_tag="PXD71_001815"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(550..552),aa:Cys,seq:gca)
     gene            complement(600..675)
                     /locus_tag="PXD71_001816"
     tRNA            complement(600..675)
                     /locus_tag="PXD71_001816"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(640..642),aa:Gly,seq:gcc)
     gene            complement(885..2309)
                     /gene="lpdA"
                     /locus_tag="PXD71_001817"
     CDS             complement(885..2309)
                     /gene="lpdA"
                     /locus_tag="PXD71_001817"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL6291907.1"
                     /translation="MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGI
                     CLNVGCIPSKALLHVAKVIEEAKNAVHNGVTFGEPTIDLDQVRAGKEAVVSKLTGGLA
                     GMAKARKVTVVEGLAAFTGPNTLVARDRDGNPTTITFDNAIIAAGSRPIQLPFIPHED
                     PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKD
                     VVAIYTKQIEKKFKLMLETKVTAVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPN
                     GKLIDAGKAGVEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASEC
                     SEGMTKLIFDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNAKAKKR"
     gene            complement(2509..4419)
                     /gene="aceF"
                     /locus_tag="PXD71_001818"
     CDS             complement(2509..4419)
                     /gene="aceF"
                     /locus_tag="PXD71_001818"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL6291908.1"
                     /translation="MSKQINVPDIGSDEVSVTEVMVKVGDTISVDQSIINVEGDKASM
                     EVPAPEAGVVKEVLVKVGDKVSTGSPMLILESADAAPAQEAPKAEAAPAAPVATTSAV
                     VEVNVPDIGGDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPVAGVVKEILIN
                     VGDKVSTGKLIMKFETASAATAQSTPVAEAAPAQASASAIKDVNVPDIGGDEVNVTEI
                     MVKVGDSVSEEQSLITVEGDKASMEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAG
                     AAPAPAAAQAASAPAPAPQAAAPAKADAAPAGQNQSGLSQEQVVASAGYAHATPVIRR
                     LAREFGVNLDKVKGTGRKGRIVKEDIQAYVKTAVQVFEKQGGTAAAATGAANGAGLGL
                     LPWPKVDFSKFGEVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITELENFRKEQ
                     NKEAEKRKLDVKITPVVFIMKAVASALEAFPRFNSSISEDAQRLTLKKYINIGVAVDT
                     PNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIGTTH
                     FTPIVNAPEVAILGVSKSEMQPIWNGKEFEPRLMLPLSLSFDHRVIDGADGARFLSYI
                     NSVLADIRRLVM"
     gene            complement(4623..7280)
                     /gene="aceE"
                     /locus_tag="PXD71_001819"
     CDS             complement(4623..7280)
                     /gene="aceE"
                     /locus_tag="PXD71_001819"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDL6291909.1"
                     /translation="MSENLRNDVDPIETQDWLASLDSLIREEGLERAQFIIEQVISQA
                     RAGGVALPTGVTTDYVNTIPVSEQPAYPGDHKIERRIRSAVRWNAIAAVLRSQKKDLD
                     LGGHISTFQSAATMYEVCFNHFFKAPTEKDGGDLVFFQGHAAPGMYGRAFLEGRITAE
                     QMDNFRQEAFTDGLSSYPHPKLMPEFWQFSTVSMGLGPVNAIYQARFLKYLENRGLKD
                     TSAQKVYAFLGDGEMDEIESKGALSFAGREKLNNLIFTISCNLQRLDGPVNGNGKIVQ
                     ELEALFTGCGWEVIKVLWGSGWDKLLEKDTSGKLKQLMMEVLDGDYLTFKSKNGAYVR
                     EHFFGRYPETAALVADMTDDEIWALRRGAHDSEKLYAAYAKAQKSDKPVVILAHQVKG
                     YRIPEAESKNTAHQSKKMSLDSLKGFRDYFELPLTDEQVESLEYVRFPEGSEEYNYLH
                     GQRKALNGYVPVRKPKFTVDFKVPELSEFQALLDEQPRGISTTMAFSRVLNTLLKDKN
                     IGQQIVPIIADEARTFGMEGLFRQIGIYNPHGQNYVPSDRDLVAYYREAKDGQVLQEG
                     INELGATASWVAAATSYSVSNLPMIPFFIYYSMFGFQRVGDMMWLAGDQLARGFMIGG
                     TSGRTTLNGEGLQHEDGHSHIQAGVIPNCVTYDPAFAFEVAVIVQDGIRRMYGENQED
                     IFYYITTLNEITEQPAMPAGAEEGIRKGLYKFETVEGKGKGHVQLLSSGAIMRHVRAA
                     AQILANDYGITADVFSAPSFNELGRDGADVARWNLLHPTETPRVPYVAQVLQDLPTVA
                     STDYMKMYAEQIRAYVPSKHYHVLGTDGFGRSDSRANLREHFEVDERYVVVAALAQLA
                     KEGTVENKVVAEAITKFGLNVDRINPLYA"
BASE COUNT         2100 a         1676 c         1387 g         2243 t
ORIGIN      
        1 ctttttatcg cccaaataaa acaaaacctc tcggaattaa atgtattttc aacggataat
       61 atttttctga attcacccta tttctcacag caaaccggtt tggtgagtgt gttcattgct
      121 gaaattgaaa aaacagttga gctattattg aaccaaactg aggtgttata tagtgaattt
      181 tacgctgaaa aattggttaa acaagttgat gcattaaaga atgccgtaga aaaaatccaa
      241 agtaagccag aatcagctca attccattct tcctatcaat tttctccaaa tattcatcgt
      301 ttagcgccca acaaacgctt acaagaatac cgtaaagcgt tacgtgcatt gaacgagaaa
      361 attagctggt tagttgagca aaatttaaat acgcaaaatg aagcaactaa acaaaccctg
      421 caaaatcaaa ttacagagac agaatatcgc aaaatgaaat gtttaaaagc aattgaagat
      481 ttagaacaag agttgctatt taaatagtga tggaggcgtg tcccggagtc gaaccgagct
      541 acatggattt gcaatccagt gcataaccgc tttgctaaca cgccataaga tttggaaatt
      601 ggagcgggaa acgaggctcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa
      661 ctgagctatt cccgcatctg aattcccaca acattgcgct gtggaacgag gtgcatttta
      721 cggatttatc attttatgtc aatgctaaat ttaacattct agattcaaat gctgaaaaaa
      781 aactcactat tcaatagttt agttttaaaa ataatcaagc ggttgtattt tgcaaaaaat
      841 ttacaaaata caaccgctta ttttttagag attaaataat ctgattaacg ctttttggct
      901 ttcgcatttg gtaagtctgt aattgaacct tcaaatactt ctgccgctaa tcctaccgat
      961 tcgtgtaacg ttgggtgagc atgaatggtt aatgcgatat cttcagcatc acagcccatt
     1021 tcgattgcaa gaccgatttc gcctaataat tcgccaccat tgctaccaac aatcgcacca
     1081 cctaatacac ggtgagtatc tttatcaaaa attaacttgg tcataccttc tgagcattca
     1141 gaagcaatcg cacgacctga agcagcccaa gggaatttcg ccacttcgta gttcaagcct
     1201 tcttgtttac actctttctc agttttacca acccaagcca cttctggttc agtataagcg
     1261 atggacggaa tgactttcgg atcgaagtaa tgtttcattc ctgcaatcac ttctgctgcc
     1321 acgtgtccct cgtgtacacc tttgtgagct aacatcggct ggccgacaat atcaccaata
     1381 gcaaagatgt gtggcacatt ggtacgcatt tgtttatcta cacgaataaa gccacgctca
     1441 tctacttcca ctccagcttt accggcatca attaatttac cgtttggcac acgtccgata
     1501 gcgactaata ccgcatcata acgtttagta tcgttgcaag ccttaccttc cattgataca
     1561 tagataccgt catctttagc ttctactgcg gttactttgg tttcaagcat tagcttgaat
     1621 tttttctcga tttgtttggt gtaaatcgct acaacatctt tatctgcagc aggaattact
     1681 tggtcgaaca tttcaaccac ttccacttct gaaccaagcg cgttatatac cgttcccatt
     1741 tctaaaccga taataccacc acccataata aggagttttt tcggtacttc ttttaattta
     1801 agagcatcgg ttgaatccca aactcgtggg tcttcgtgtg gaataaatgg tagttgaatc
     1861 ggacgagaac ctgctgcgat aatcgcatta tcgaacgtga tagttgtagg attgccgtca
     1921 cgatcacgag cgactaacgt attcggacca gtaaatgcag ccaagccttc tactactgtc
     1981 actttacgag ccttagccat tcctgctaag ccaccggtta attttgaaac aacggcttct
     2041 ttaccagcac gtacctgatc taaatcaatc gttggctcac caaaggtcac accgttgtgt
     2101 actgcatttt tagcttcttc aattactttt gcaacgtgta ataatgcttt agaagggata
     2161 caacctacgt ttaagcaaat accacctaag gttgaataac gctcaacaat tactgtttct
     2221 aagcctaagt cggcacaacg gaatgccgct gagtaacctg ctggacctgc accaagtacg
     2281 acaacttggg ttttaatttc tttgctcata gtttttcctc tttttattcc ccccctttag
     2341 caaagagggg taagggagat ttggcagaat ataaagcgaa gtgagagagc aatcaatcaa
     2401 acacaaattt ctaccaaata atttattctc ttcgtaaatt acgtctatca aatctccccc
     2461 tacccctctt ttctaaagag ggactttttt aggaggagct ttaccgaatt acatcactaa
     2521 acgacgaata tctgccaaca cgctattaat atagcttaag aatctcgcac catcagcacc
     2581 atcgatcaca cggtggtcga atgataatga aagcggaagc attaagcgtg gttcaaactc
     2641 tttgccattc caaatcggtt gcatttcaga tttagacaca cctaaaatag ccacttccgg
     2701 tgcattcacg attggcgtaa agtgcgttgt accaataccg cctaaacttg aaattgtgaa
     2761 acagccacct tgcatatcag atgctgtcaa tttaccttca cgggcttttt tcgagacttc
     2821 cattaattcg cgagaaagtt cgataatgcc ttttttgttc acatttttga acaccggcac
     2881 cactaaaccg tttggcgtat caaccgcaac cccgatattg atgtattttt tcaaggttaa
     2941 acgttgtgca tcttcagaaa ttgagctgtt gaaacgaggg aaggcttcta atgcacttgc
     3001 cactgctttc ataatgaaga ccacaggggt aattttcacg tctaatttac gtttttccgc
     3061 ttctttattt tgctctttac ggaagttttc taactcggta atgtctgtgc gatcgaaatg
     3121 tgtaacgtga ggaatcatta cccagttacg gtgtaagttc gcaccggaaa tcttattgat
     3181 acggcttaat tcaacttctt caacttcacc aaacttgctg aaatctactt tcggccacgg
     3241 taataaacct aagcctgcac cgtttgccgc acctgttgct gccgctgctg ttccgccttg
     3301 tttttcaaat acttgaacag ccgttttaac gtatgcttga atatcttctt taacgatacg
     3361 gcctttacga cctgtacctt ttactttgtc taggtttaca ccaaactcac gagctaaacg
     3421 gcgaattacc ggtgttgcgt gtgcatagcc agctgaagcc accacttgtt cttggcttaa
     3481 acctgattgg ttttgacctg ccggagctgc gtcagctttc gccggagctg ctgcttgtgg
     3541 agcaggagca ggagctgaag ccgcttgagc agccgccgga gccggtgccg cacccgctac
     3601 ttcaaatttc ataattaatg aaccggttga aactttatca cctgatttca ctaaaatttc
     3661 tttcaccaca ccggcaaatg gagccggaac ttccattgaa gctttatcac cttcaaccgt
     3721 gattaaagat tgttcttctg aaacagaatc acccacttta accataattt cagttacgtt
     3781 tacttcatca ccaccgatgt ccggtacatt tacatcttta atcgctgatg ccgaagcttg
     3841 agctggagca gcttccgcaa ccggtgtaga ttgtgctgta gctgcggaag ccgtttcaaa
     3901 tttcataatc aatttaccag ttgaaacttt gtcacccaca ttgattaaga tttcttttac
     3961 tacgccggca accggtgcag gcacttccat tgaggcttta tcaccttcaa cgttgatgat
     4021 agattgatcc acttcaacgc tatcacctac tttaaccata atttcggtta cgttaacttc
     4081 atcgccaccg atatcaggta catttacttc aacaacggct gaagttgttg ctactggagc
     4141 agcaggagct gcttctgctt ttggtgcttc ttgagcagga gcagcatctg ctgactctaa
     4201 aattaacatt ggtgaaccgg ttgaaacttt atcacccact ttcactaaaa cttcttttac
     4261 cacacctgct tcaggagccg gcacttccat agaggcttta tcgccttcta cgttaataat
     4321 tgattgatct actgaaatag tatcaccaac ttttaccatc acttcagtca cactgacttc
     4381 atccgaaccg atgtccggca cattaatttg ttttgacatt ttgaaatcct tcttgagtta
     4441 ccccctctcc cattgtggga gggacagatt tttctttgta agaagaaaaa tcagggagaa
     4501 gggaatgcaa gcggttgatt ttctcaacat ttttgcaaat aaaactaata ccctctctgc
     4561 taaaagtttc taaacgaaac ctttagctgt ctctctccca tcaagggaga gagtattaga
     4621 aattacgcat ataatgggtt aatacgatct acatttaagc cgaatttagt aatagcttcc
     4681 gcaaccactt tgttttcaac tgtaccttct ttcgctaatt gagcgagggc tgcaactact
     4741 acataacgct catcaacttc gaagtgttca cgtaagtttg cacggctgtc tgaacgaccg
     4801 aaaccgtctg tacctaacac gtgatagtgt ttacttggta cataagcacg gatttgctct
     4861 gcgtacattt tcatatagtc ggttgaagca acagtcggta aatcttgtaa cacttgagca
     4921 acgtacggta cacgtggtgt ttcagttggg tgtaataagt tccaacgagc cacatctgct
     4981 ccatcacgac ctaattcatt gaatgatggt gctgagaata catctgctgt aatgccgtag
     5041 tcattcgcga gaatttgtgc agctgcacgc acgtgacgca taattgcacc tgagcttaat
     5101 aattgtacgt gacctttacc tttaccctca acggtttcaa atttatataa acctttacgg
     5161 ataccttctt ccgcacctgc cggcattgcc ggctgttcgg taatttcatt taaggtagta
     5221 atgtagtaga agatatcttc ttggttttca ccatacatac gacgaatacc gtcttgaaca
     5281 ataaccgcaa cctcaaaggc aaatgcaggg tcataagtga cgcagttcgg gattacacct
     5341 gcttgaatat ggctatgacc atcttcgtgt tgtaaacctt caccgtttaa tgttgtacga
     5401 cctgaagtac caccaatcat aaagccacga gctaattggt caccggctaa ccacatcata
     5461 tcgcctacac gttggaaacc aaacattgag tagtagatga agaatgggat catcggtaag
     5521 ttgctaacag aataagaagt cgctgctgcc acccaagagg ctgttgcacc taactcattg
     5581 ataccttctt gtaacacttg gccatctttt gcttcacggt agtaagctac taaatcacgg
     5641 tctgacggta catagttttg accatgtggg ttataaatac cgatttgacg gaataaacct
     5701 tccataccga aagtacgggc ttcgtccgca ataataggta cgatttgctg accgatattt
     5761 ttatctttca ataaagtatt caatacacga gagaacgcca ttgtggttga aataccacgt
     5821 ggttgctcat ccaataatgc ttggaattcg cttaattcag gcactttgaa atcaaccgta
     5881 aatttcggtt tacgtacagg cacataaccg tttaaggctt tacgttgtcc gtgtaagtag
     5941 ttgtactctt cagaaccttc agggaatctt acatactcta ggctttctac ttgctcatcg
     6001 gttaatggta gctcgaagta gtcacggaag ccttttaagc tgtctaatga catttttttc
     6061 gattggtgag cagtattttt actttccgct tcaggaatac ggtagccttt cacttggtga
     6121 gctaaaatta caactggttt gtccgatttt tgtgcttttg cataggctgc ataaagtttt
     6181 tcagaatcgt gtgcaccacg gcgtaatgcc caaatttcat cgtctgtcat atcagcaact
     6241 aatgctgcag tttcagggta acgaccgaag aagtgttcac gaacgtaagc accatttttg
     6301 gatttgaagg ttaaatagtc accatcaagc acttccatca ttaactgttt aagtttgcct
     6361 gaagtatctt tttcaagcag tttatcccaa ccactacccc ataacacttt aatcacttcc
     6421 cagccacaac cggtaaagag tgcttctaat tcttgcacga ttttgccgtt accgttaacc
     6481 ggaccgtcta aacgttgtaa gttacagcta atagtgaaga ttaagttatt taatttttca
     6541 cgaccggcga atgataatgc acctttagat tcgatttcat ccatttcgcc atcgcctaag
     6601 aatgcatata ctttttgagc cgaagtatct tttagaccac ggttttctaa atattttaag
     6661 aaacgagctt ggtaaatagc atttaccggt cctaaaccca tagatacggt agagaattgc
     6721 caaaattcag gcattaattt agggtgtggg taagaagata aaccgtctgt aaatgcttct
     6781 tgacggaagt tatccatttg ttctgcggta atacgacctt ctaagaacgc acgaccatac
     6841 atacccggtg ctgcgtggcc ttggaagaac actaaatcac caccatcttt ttcggttggt
     6901 gctttgaaaa agtggttaaa acacacttca tacatagttg ccgcagattg gaaagttgaa
     6961 atatgaccac ctaagtcaag atctttcttc tggctacgaa gtactgccgc aatcgcattc
     7021 caacgtaccg cagaacgaat acgacgttcg attttatgat cacccggata agctggctgc
     7081 tcggaaacag ggatagtatt gacataatct gttgttacgc ctgttggtaa tgccacgcca
     7141 cctgcacgag cttggctaat aacttgttca atgataaatt gagcgcgctc taaaccttct
     7201 tcacgaatta atgaatcgag tgatgctaac caatcctgag tttcgattgg atctacgtca
     7261 tttcttaagt tctctgacat agtattcttc cttataaatt aacgattgag gtgataaata
     7321 acaaaatttt aacaaatttt gtttgtttgg tgatttttag accaattctt atcgaaaaaa
     7381 tttcagtaaa acaacaattt ccgaat
//
LOCUS       DANLXS010000115         7385 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.115, whole genome shotgun sequence.
ACCESSION   DANLXS010000115 DANLXS010000000
VERSION     DANLXS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.115"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            64..2520
                     /gene="thrA"
                     /locus_tag="PXD71_001820"
     CDS             64..2520
                     /gene="thrA"
                     /locus_tag="PXD71_001820"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDL6291910.1"
                     /translation="MRVLKFGGTSLANPERFMQAAEIIEKAHLADQAAGVLSAPAKIT
                     NHLVAIVDKAIAGESFQSNLSEATEIFHNIINGLYATNNHFAKEALLALVEAELNQIQ
                     QVAAEAAQTKSLSDKIAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
                     LESSVDIAESTKRVDAGSIPKKNIVLMAGFTAGNEQGELVLLGRNGSDYSAACLAACL
                     KADVCEIWTDVDGVYTCDPRLVPDAVCLETMSYQEAMELSYFGAKVIHPRTIGPLVPL
                     NIPCLIKNTGNPDGKGTLIDGNVANDNLKVKGITNLDNVAMFNVSGSGMQGMVGMAAR
                     VFSTMSKAGISVILITQSSSEYSISFCVPAKSAEKALAALNTEFAQELSENLLDPIDI
                     IKDLSIVSVVGDGMRTAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPLNKAIE
                     AVKATHKSLFNSKRVVDMFLVGVGGVGSELIEQVKQQKEFLAKKDIEIRVCALANSDK
                     MLLNENGLNLDNWKEDLEKAIQPSDFDVLLSFIKFHHVVNPVFVDCTSAQSVANLYAR
                     ALKEGFHVVTPNKKANTSGYDYYLEMRENARQSQHKFLYETNVGAGLPVIENLQNLLA
                     AGDEVEKFEGILSGSLSFIFGKLEEGLTLSQATLIAKEKGFTEPDPRDDLSGADVARK
                     LLILVRETGLPLEFEDIEIEGVLPKGFSEGMSRDEFLQALPSLDEEFSKRVEAAKAEG
                     KVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYNPIPLLLRGYGAGN
                     SVTAAGIFADILRTLQGGVN"
     gene            2520..3467
                     /gene="thrB"
                     /locus_tag="PXD71_001821"
     CDS             2520..3467
                     /gene="thrB"
                     /locus_tag="PXD71_001821"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDL6291911.1"
                     /translation="MALRIYAPASSANISVGFDSLGAAISPIDGSLLGDVVQIEESEQ
                     QFELESAGYFVRKLPKEPQKNIVYQAYVLFSERLKLRNGSVKNLRLTLEKNMPIGSGL
                     GSSACSIVAALVALNKFHDEPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGLQLM
                     TQSLGNICQPLPFFDEWYWVLAYPGVEVSTAEARAILPKSYTRQDMIQQARYLGSFVH
                     ACHTHQDILAATMMKDLIAEPYREDLLPNFPAVRQGCKDLGALAVGISGSGPTMFAIA
                     PDLEHAQRLVSYLEKEYLQNNEGFIHICKVDNQGARELK"
     gene            complement(3582..4958)
                     /gene="cysS"
                     /locus_tag="PXD71_001822"
     CDS             complement(3582..4958)
                     /gene="cysS"
                     /locus_tag="PXD71_001822"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL6291912.1"
                     /translation="MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTF
                     VSFDVIARYLRYSGYNLRYVRNITDVDDKIIKRALENNETCDELVDRMIAEMHKDFDA
                     LNILRPDVEPRATKHIPEIIAMVEKLIHNGHAYVAADGDVMFDVESFQKYGALSRQNL
                     EQLQAGARVEIKSIKKNPMDFVLWKMSKAGEPSWESPWGNGRPGWHIECSAMNSKELG
                     EHFDIHGGGSDLMFPHHENEIAQSCCAHGGDYVNYWLHTGMLTIDKEKMSKSLGNFFS
                     IRHMLGLYDAESLRYFFLTAHYRSLLDYSIDNLDLARSALERLYTALRGCDVSGEAIG
                     EHYVEAFKTAMDDDFNTPGALAVLFEIARELNKLKTEDMAKANGLAVRLKELAGVLGL
                     LEQDPDAFLKGDANDDETAEIEALIKQRNEAKAAKNWAVADEVRDKLKAINIVLEDTP
                     NGTIWRKA"
     gene            5114..5623
                     /locus_tag="PXD71_001823"
     CDS             5114..5623
                     /locus_tag="PXD71_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6291913.1"
                     /translation="MITLHTNFGDIKIELNFEKAPITAKNFEDYAKEGFYNGTIFHRV
                     IDGFMIQGGGMTAGMAEKPTKDPIQNEANNGLSNKRGTIAMARTSDPHSASSQFFINV
                     ADNTFLDYRSKEMFGRSVVQDWGYAVFGEVVEGMDVVDKIKGVKTGNKGFHQDVPVED
                     VVIESVSVA"
     gene            complement(5715..6644)
                     /locus_tag="PXD71_001824"
     CDS             complement(5715..6644)
                     /locus_tag="PXD71_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL6291914.1"
                     /translation="MEIALLLADKILQLTLMVLLGYLIVKTKLLKSEHSYPISVIGLY
                     IISPAMLITAFQVDYTTEILNGLYLAFLISVLLNGLLILLGWLLKRALKLDNIEQATA
                     IYSNSGNLIIPIVASLFGNEWVIYSTAFIVVQNVLFWSHLRLLICGKGNVPFRKMIFN
                     INIIAIVIGMMLFLLHIKLPATLANTLSTLGHMLGPLAMLVAGMLIAAIPFKEIITDK
                     RIYLVAFLRLIFIPLILLAVIKLGGFASWIENGKTVVMITFLATISPSAATATQMAVA
                     YGQDAKKASAIYGVTTLLCVFTMPLIIWLYQFL"
     gene            complement(6647..7366)
                     /gene="fdhD"
                     /locus_tag="PXD71_001825"
     CDS             complement(6647..7366)
                     /gene="fdhD"
                     /locus_tag="PXD71_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDL6291915.1"
                     /translation="MPVALVYNGISHTVMMATPANLEDFALGFSLAEGILNAPSELYG
                     LDVIEQENGVEIQLEISSRQFVALKDKRRSMAGRTGCGICGVEQLEQVNQSCKIVKKN
                     NRLQNVNPLILEDCLLQLENAQFLSKETGASHAAAFFTPDGKLLAIREDVGRHVALDK
                     LIGWYAKADFPIGFVFVTSRASFEMVQKCLAVGIEMLCAISATTEMAVNMARENQFTL
                     LAFARKGKATIYAGEERLALN"
BASE COUNT         2165 a         1587 c         1572 g         2061 t
ORIGIN      
        1 gaaaacccgc tacaagcggg tttttttgta taaaaattta caaataattt atcaggagta
       61 actatgcgag ttcttaaatt tggaggcact tctcttgcca accctgaacg ctttatgcaa
      121 gcggcagaga ttatcgagaa agctcatttg gcagatcaag ctgccggcgt attatccgcc
      181 ccagccaaaa ttaccaatca tcttgtcgct attgtagata aagcgattgc cggtgaatca
      241 tttcaatcta acctttctga agctaccgag attttccaca acatcattaa cggcttatat
      301 gctacaaata atcactttgc taaagaagca ctgttagcct tagtggaagc ggaattaaac
      361 caaattcagc aagtggctgc agaagcagct caaactaaat cgttgtcaga taaaattgct
      421 gccactattc actgccgtgg tgaaaaactc tctattgcga tgatgcaagc gtggttcgag
      481 gcaaaaggct atgaagtcac tcgtatcgac cctgttgaaa aattattggc tcacggcagc
      541 tatttggaat cttcggtcga tattgccgaa tccaccaagc gagttgatgc aggttcaatt
      601 ccaaagaaaa atatcgtgct aatggctggt tttaccgcag gtaatgagca aggcgaactg
      661 gtgttattag ggcgtaacgg ttcagattat tctgccgctt gtttggcagc gtgtttaaaa
      721 gccgatgttt gtgaaatctg gacggacgta gatggcgttt acacctgcga ccctcgtttg
      781 gtgcctgatg cagtatgctt agaaacaatg agctatcaag aggcaatgga gctttcctac
      841 tttggggcga aagtgatcca ccctcgcacc atcgggccat tagtgccgct caatattcct
      901 tgtttaatca aaaataccgg caatccggac ggaaaaggca cattaattga tggcaatgtg
      961 gcaaacgata atctcaaagt aaaagggatt accaaccttg ataacgtggc gatgttcaac
     1021 gtttcaggtt caggaatgca gggaatggtg ggcatggctg cccgtgtgtt ctcgacgatg
     1081 tcaaaagccg gtatttcggt gattttaatt acccaatcat catcagaata tagcatcagc
     1141 ttctgcgtgc cggcaaaatc ggcagaaaaa gcgttagcgg ctctaaatac tgaatttgca
     1201 caagagttat ctgaaaactt acttgacccg attgatatta ttaaagattt atccattgtt
     1261 tcggtagtgg gtgatggtat gcgtaccgcc aaaggaattg cggctcgctt cttctctgcc
     1321 ctagcccaag ccaacatcag tattgtggca attgcacagg gttcatctga gcgttccatt
     1381 tctgctgtcg tgccgttaaa caaagcgatt gaagcagtaa aagcaactca caaatcgcta
     1441 tttaacagta aacgtgtggt ggatatgttt ttagtgggtg taggcggtgt gggtagcgaa
     1501 ctcatcgagc aagtcaaaca acaaaaggag tttttggcaa agaaggatat tgagatccgt
     1561 gtgtgtgctt tagctaattc agataaaatg ttactcaacg aaaacggctt aaatttagac
     1621 aactggaaag aagatttaga aaaagcaatt cagccgtcag actttgatgt attgctttct
     1681 ttcatcaaat tccaccacgt ggtaaaccca gtatttgtag attgcacttc ggcacaatcg
     1741 gtggcgaacc tgtacgcacg agccttaaaa gagggcttcc acgtggtaac accaaacaaa
     1801 aaagccaata catcaggtta cgattactac ttagaaatgc gtgaaaacgc ccgccaaagc
     1861 cagcacaaat tcctctacga aactaatgta ggggcaggtt taccggtaat tgaaaactta
     1921 caaaacctat tagctgccgg tgacgaagta gagaaatttg aagggatttt atccggctcg
     1981 ctctctttca ttttcggcaa attagaagaa ggtttaaccc tttcgcaagc aactttaatc
     2041 gccaaagaaa aaggctttac cgagccagat ccacgagatg atttatccgg tgcagatgtt
     2101 gctcgtaaac tcttaattct tgttcgtgaa accggcttac cgcttgaatt tgaagatatt
     2161 gaaattgaag gcgtattgcc aaaaggcttc tcggaaggaa tgagtcgtga tgagttttta
     2221 caagctctac cgtctttaga cgaagaattc agcaagcgtg tagaagctgc caaggctgaa
     2281 ggcaaagtgt tgcgttatgt gggctcgatc acaggtgata aatgccgagt tgccattgaa
     2341 gccgtggacg aaaaccaccc actttacaaa gttaaagacg gcgaaaatgc ccttgctttt
     2401 ctcacacgtt actacaaccc tattccatta ttattgcgtg ggtatggtgc gggcaatagc
     2461 gtaaccgctg ccggtatctt tgcagatatt ctgcgtactt tacaaggagg agtgaactaa
     2521 tggcgttacg aatttatgcc ccagcttcaa gtgcaaatat tagtgtgggt tttgactcat
     2581 taggcgctgc aatctccccg attgacggct ctttgctcgg cgatgtagtt caaattgaag
     2641 agagtgaaca acaatttgag ctagaaagtg caggttattt tgtacgtaaa ttgcccaaag
     2701 aaccacaaaa aaacattgtc tatcaagcct atgtgctatt tagcgaacgc ttgaaattac
     2761 gtaacggcag tgtaaaaaat ttacgcttaa cacttgaaaa aaatatgccg attggctcag
     2821 ggctaggctc aagtgcctgc tcgattgttg cagcattagt ggcactcaat aaattccacg
     2881 atgaaccgtt ctccaaaatg gaattactgg aaatgatggg cgaattggaa ggcagaattt
     2941 caggctcaat ccactacgac aacgttgccc cttgctattt aggtggcctg caattaatga
     3001 cacaatcgct cggcaatatt tgccaacctc tgccattctt tgatgaatgg tattgggtgc
     3061 ttgcctaccc gggcgtggaa gtatcaaccg ccgaagcccg tgctattttg ccgaaaagct
     3121 atactcgtca agatatgatt cagcaagccc gctacttagg atctttcgtc catgcttgcc
     3181 atacacatca agatatcttg gctgctacaa tgatgaaaga cctaattgct gagccttacc
     3241 gtgaagattt actgccgaac tttccggctg ttcgtcaagg ctgtaaagac ttaggtgcgt
     3301 tagcggttgg tatttcaggt tccggtccga caatgtttgc gatagcccct gatctcgaac
     3361 acgctcaacg tttagtgagc tatttagaga aagaatatct gcaaaacaac gaaggcttta
     3421 ttcatatctg taaagtcgat aatcaaggag caagagagct gaaatagccc tctcgaacaa
     3481 ccatatcact aaataactta aaggactgac tccaatacca cttggagcag ccctttttga
     3541 ttaatttttt gaaaagtcta tcacttataa acaatcgaag gctatgcctt acgccaaatt
     3601 gtgccgtttg gggtgtcttc gaggacaata tttatcgctt tgagcttatc tcgcacttca
     3661 tcggcaaccg cccagttttt agccgctttg gcttcgttgc gttgtttgat taaggcttcg
     3721 atttctgccg tttcatcgtc attcgcatca ccttttaaaa acgcatcggg gtcttgttcc
     3781 agtaagccta gcacgccggc taactctttt aaacgaacgg ctaaaccgtt ggcttttgcc
     3841 atatcttcgg ttttgagttt gttcaactca cgggcaattt caaataacac cgctaatgcc
     3901 cccggggtgt tgaaatcatc gtccattgcg gttttaaagg cttctacata gtgttctccg
     3961 atggcttcgc ccgacacatc acaaccacgc agagcggtat ataaacgctc aagagccgaa
     4021 cgggctaaat caaggttgtc aatgctgtaa tctaataacg aacggtaatg agcggttaag
     4081 aagaaataac gtaacgattc ggcatcgtat aagcctaaca tatggcgaat gctgaagaaa
     4141 ttgcccaaag atttcgacat tttttcttta tcaatggtaa gcattccagt gtgtaaccaa
     4201 taattgacat aatcgccacc gtgagcacag caagattgtg caatttcgtt ttcgtgatgc
     4261 gggaacatta aatccgagcc accgccgtga atatcaaaat gttcgcccaa ctctttgcta
     4321 ttcattgccg aacattcaat atgccagccc ggtctgccat tgccccacgg gctttcccag
     4381 cttggctcac cggctttcga cattttccac aagacaaaat ccatcgggtt tttcttgatg
     4441 gatttaattt caacccttgc accggcttgt agttgctcta aattttggcg ggataatgca
     4501 ccatatttct ggaacgattc cacatcaaac atcacatcgc catcggctgc gacataagcg
     4561 tgtccgttgt gaatcagttt ttctaccatc gcaataattt caggaatatg cttggtggct
     4621 cgtggctcaa cgtccgggcg aagaatgttt aacgcatcaa aatctttgtg catttcagca
     4681 atcattctat ccactaattc atcgcaagtt tcgttatttt ctaacgcacg tttgatgatt
     4741 ttgtcgtcca catcggtaat gttacgcaca tagcgtaaat tgtagcctga ataacgtaaa
     4801 taacgggcaa tcacatcgaa agagacaaaa gtgcgaccgt gcccaaaatg acaaagatcg
     4861 taaaccgtga cgccacagac gtacattccc acctgatttg ggttaatcgg tttaaattct
     4921 tctttttcac gttttaaggt gttataaatt ttgagcataa agtcctcttg tgtaacattc
     4981 agtaacaaat ggggctaagt atagcgaata agcggtctga ttttgagatt tttttgcaat
     5041 ttggcgtaaa ctttttaata atagcgagcg aaatttattt tccattccat ttttatttat
     5101 aaaggaacaa agaatgatta cattacatac caatttcggt gacattaaaa tcgaattaaa
     5161 ctttgaaaaa gccccgatta ccgctaaaaa ctttgaagat tacgctaaag aagggtttta
     5221 taacggcacg attttccacc gtgtgattga cggctttatg attcaaggtg gcggaatgac
     5281 ggcaggtatg gctgaaaaac cgacaaaaga tccgattcaa aacgaagcga acaacggttt
     5341 aagcaacaag cgtggcacaa tcgcaatggc tcgcacttct gatccgcatt ctgcttcgtc
     5401 acagtttttc attaatgtgg cggataatac tttcttagat tatcgctcaa aagagatgtt
     5461 cggcagaagt gtagtgcaag attggggcta tgcagtattt ggcgaagtgg ttgaaggtat
     5521 ggacgtggtc gataaaatca aaggcgtaaa aaccggcaat aaaggcttcc accaagacgt
     5581 gccggttgaa gatgtggtga ttgagtctgt aagcgttgcc taattccgca aattagccta
     5641 caagcggttg atttttcaca attttttgca tttctcagcg ttgcaaaaaa gtagcagaaa
     5701 tcgactgctt gtttttatag aaattgatac agccaaataa tcagcggcat tgtaaacaca
     5761 caaagtaaag tggttacgcc ataaatcgca ctggcttttt tggcatcttg cccgtaagcc
     5821 accgccattt gggtcgccgt tgcagcagaa gggctgatgg tggctaagaa tgtaatcatt
     5881 accaccgttt ttccattctc tatccagctc gcaaaaccgc cgagtttaat caccgctaac
     5941 aaaatcagcg gaataaaaat caagcggaga aaagccacca agtaaatgcg tttatcggta
     6001 ataatctctt taaatggaat tgctgcaatt aacatccctg ccacaagcat tgctaaaggc
     6061 cctaacatat gccctaaagt tgagagcgtg tttgcaagcg tagcgggcag tttgatatgt
     6121 agcaaaaaca acatcatacc aatcacaatc gcaataatgt tgatattaaa gatcatcttt
     6181 ctaaacggca cattgccttt gccacaaatc agcagccgta agtgagacca aaacaacaca
     6241 ttttgcacca caataaaagc cgttgaataa atcacccatt cattgccgaa cagggaggca
     6301 acaatcggaa taattaagtt gccggagttg gaataaatag cggtggcttg ttctatgttg
     6361 tctaatttca acgctctttt gagcagccaa ccaagcagaa ttaacaagcc gtttaataag
     6421 acggagatta aaaatgccag atacaagccg tttagtattt cggtggtgta atctacttga
     6481 aaagcagtaa tcagcattgc cgggctgata atgtaaagtc cgataaccga aatcggataa
     6541 ctgtgctccg atttgagtaa tttagtttta acgatgaggt agccgagcag caccatcagg
     6601 gttaattgta gaattttatc ggctaataaa agggctattt ccattatcaa ttaagagcga
     6661 gtctctcttc tcctgcataa atggtcgctt tgcctttgcg ggcaaaagca agtaaggtaa
     6721 attgattttc acgagccata ttcactgcca tttcagtggt cgctgagatg gcacacaaca
     6781 tttcaatgcc aacggctaag catttctgca ccatttcaaa gctggcacga ctggttacaa
     6841 acacaaatcc aatcggaaaa tcagctttag cataccaacc gattaattta tccaacgcca
     6901 cgtgtctgcc cacatcttca cgaatggcaa gcagttttcc atccggggta aaaaatgcag
     6961 cggcgtggct tgcaccggtt tctttcgata agaattgagc attttctagc tgtaataaac
     7021 aatcctctaa aatcagtgga ttgacgttct gcaagcggtt atttttctta acaattttgc
     7081 aactttgatt aacctgctct aattgctcta caccgcaaat accgcaacct gttctgcctg
     7141 ccattgagcg gcgtttatct tttaatgcaa caaattggcg gctcgatatt tcaagctgaa
     7201 tttctactcc attttcttgt tcaattacat ctaaaccata taattccgat ggggcattta
     7261 aaatcccttc cgccaatgag aatcctaaag cgaagtcctc caaattagcc ggtgttgcca
     7321 tcattacagt atgagaaata ccgttatata ccaaagcaac cggcacttct gaaattaaat
     7381 tttct
//
LOCUS       DANLXS010000116         7360 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.116, whole genome shotgun sequence.
ACCESSION   DANLXS010000116 DANLXS010000000
VERSION     DANLXS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.116"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(30..1085)
                     /locus_tag="PXD71_001826"
     CDS             complement(30..1085)
                     /locus_tag="PXD71_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6291916.1"
                     /translation="MGRPRKQQNRYLPENLRPKTVTRKSGKTVIYWLYRTHGKTEISL
                     GTNRNEAFIKAAQLNIERESQTARITFGLVAKRYRDEVIPQKKASTAQTDLCKIKNLL
                     LFFNDAPLEEITPQHIREYMTWRKDTPGSANNEFSMFSHIWQYAREWGYTNLPSPNIG
                     MKRYPIRKREIYVENNIFQMVYEVADQDMRDLMDLAYITGQRPVDLVNIRKEHIFDGY
                     LHIVQQKTQAKLRIEIVGKLADIIQDRLEKATGTHLFSTKKGEKLTARKLGKRFTYIR
                     EQAVKNNPEYANEIMAFQFRDLRAKSGTDKALSLGEEAARQQLGHTSVQMTKTYIRKA
                     PIVTPIKESVPSSKHQS"
     gene            complement(1045..1320)
                     /locus_tag="PXD71_001827"
     CDS             complement(1045..1320)
                     /locus_tag="PXD71_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL6291917.1"
                     /translation="MEQPTEFLTKDELADLTGYRQKKRQIEWLRERHYIIQDENKYKP
                     LVLYKDVYGHPRQQYPEAQSTGKWKSPAYLMGVKNGKTTQTTESLLT"
     gene            complement(1410..1916)
                     /locus_tag="PXD71_001828"
     CDS             complement(1410..1916)
                     /locus_tag="PXD71_001828"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL6291918.1"
                     /translation="MRILPRNENYAATENGEIFSFLSNKILKPRPLKNGYLRVQLVDE
                     NGVKDYLIHRLVCETFHGYPDLHVNHKDRDKTNNRPENLEWCTRSENMLHCSRLGGFS
                     KQSERMKKMNIESCSVPVAAITLDGKLFKKYTSMTSAGKDGFSHSKISLCIQGKRKTH
                     KGYIWQKL"
     gene            complement(1894..2490)
                     /locus_tag="PXD71_001829"
     CDS             complement(1894..2490)
                     /locus_tag="PXD71_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL6291919.1"
                     /translation="MPLSITANLTKAVQLKHQGEALSGGSETLVVHGTQDPIISQNKA
                     HCLGRNNGQENVLFEVKGAEAVRISEIQDKTPTLKARMGTGGNNIPCIALAGNTIGRQ
                     PQNDGNGNVFDESGVSYMLTRTDVHAVSAGTTIRKLTPTECERLQGFPDGWTKITYRG
                     KSAEECPDSPRYKAIGNSMCTNVMKWIGERLSAYLTSQ"
     gene            complement(2411..3730)
                     /locus_tag="PXD71_001830"
     CDS             complement(2411..3730)
                     /locus_tag="PXD71_001830"
                     /inference="COORDINATES: protein motif:HMM:NF024795.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HDL6291920.1"
                     /translation="MNNIIKFTYGSICSGIEAASVAWHDIGTPLWFSEIEPFPCAVLA
                     HRFPDVPNLGDMTALPEKILNRKIPAPDVLVGGTPCFTAGHMVLTDKGYMPIETLSVG
                     DLVVTHKGQLKPILRVGSEIKPVGKLTAVGLPESIVCTPEHPFYAQKWQTINTKRNGK
                     YHRKTIISKPEWIEAHKLEGYQWVSLTDFSVMPYGGFHSKLTDDEVLLIAGYYLGDGW
                     IRRWKGKNKKAVVISVNKEKYRKFSLSFAKLPHHITTENNSVIKITICDTELADFLYS
                     QFGEKAGGKTIPAWCLSHSFRSKIFEGYMITDGSFRNGVYTANSISKSLAYGIAALSQ
                     TLGYISSVSKVTVAPTKEIQGRIVNQNDYYQMRAFLQSTSRKSRLDENRLLRSVQSFE
                     ITGNEVVYNIEVADDNSYILNNAVVHNCQSDKGGTVKASGGGFEWRE"
     gene            complement(3727..4632)
                     /locus_tag="PXD71_001831"
     CDS             complement(3727..4632)
                     /locus_tag="PXD71_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDL6291921.1"
                     /translation="MQFQEHNNRKKADKFAEYITGEALRRYVAEKVKQYARNNVSVFD
                     GAAGSGQLEQFVRPTSFTAVEVQSESCDVLRHNFPTAQIYNQSFFLYPNGEPCDCVIM
                     NPPFSLKFKDLSQAEQAQIQSEFPWKKSGVVDDIFVLKGLANSHQWGFFILFPGVGYR
                     QTEKRFREEVGNQLVELNRIQNAFEDTPIEVLFLVVDKTKTTDDCSRELIDCAGGQIQ
                     QICADKWKIRPDHWESIQPPPPPQEEIKPLELEAFAREQAIERIVGEVRLSKLIAEFF
                     EPELRRTFNAFIDDICVAVQAEKIV"
     gene            complement(4866..5543)
                     /locus_tag="PXD71_001832"
     CDS             complement(4866..5543)
                     /locus_tag="PXD71_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL6291922.1"
                     /translation="MSNQVQFPVFNFNSSAVRVIIDPNQEPWFCGADVCRILGYVNES
                     LTLQKHCKENGVSKRYLTDKMQRQQKAIFINEPNLYRLIIKSRKPEAEKFEAWVFEEV
                     LPQIRKTGKYALQNNQQNLPLAPPPKKYSFDFTEDELQSLIWAWFAFVRGIHTFRYIY
                     PMFQKLGSNIAPEIYGQGFEYSHTAQSAHKILERITKEFDYDPMTNWRVLKHVRGFDP
                     AFKKPTF"
     gene            complement(5791..6630)
                     /locus_tag="PXD71_001833"
     CDS             complement(5791..6630)
                     /locus_tag="PXD71_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDL6291923.1"
                     /translation="MANSVMIVQGVEVKVTARDGEDYISLTDMCKAFGDSDQLIKNWL
                     QNKNTIEFLQVWEELNNPNFNLVELHQIKNNIGLNRFVMSVKKWTATGAIGLVAKAGR
                     YGSGTYAHKDIALEFGSWLSPEFKLYLIKEFQRLKQQEANESKLEWSVKRILSKANYR
                     IHTDAIKAHIIPALLNTKQHTFVYSSEADILNQALFGQTAKQWKDRNPNLKGNMRDHA
                     TVEQLTVLAALESQNALLIEQGYSQEERLAMLNRLAIQQMSSLLQTRAIEELKEKPLL
                     IEE"
     gene            6860..7237
                     /locus_tag="PXD71_001834"
     CDS             6860..7237
                     /locus_tag="PXD71_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291924.1"
                     /translation="MFSLLRDALTFAAMFTGILFPAIIHKYDLSLGTQIQLLSFVPAF
                     FVLCHFLIKRKYQRDFDRLSYLSAVVCVAMCAGVGAFSQSEMIELGFKVERVLDYVTI
                     KFLLYFWSLLLLPVVLRKLFGKD"
BASE COUNT         1892 a         1491 c         1515 g         2462 t
ORIGIN      
        1 aaaaatgcaa aatgttccga aactgtcttt taagactggt gttttgagga gggaacactt
       61 tctttgatag gggttactat tggggctttt cgaatgtatg ttttggtcat ttgaacgcta
      121 gtatgaccta gttgttggcg agcggcttct tcacctagag aaagggcttt atctgttcct
      181 gatttagctc ttagatcccg aaattggaat gccattattt catttgcgta ttccgggtta
      241 ttcttgaccg cttgttcccg aatataagtg aaccttttac ctagttttct agcggttagc
      301 ttttcccctt tcttagtgct gaataagtgt gttccagtag ctttctccag cctatcttgg
      361 ataatatcag ccagttttcc cactatctca atacgtagtt tggcttgtgt tttttgctga
      421 acaatatgga gataaccatc aaaaatgtgt tctttccgga tattgaccaa atcgacagga
      481 cgttgtccgg tgatataggc gagatccatt aagtctctca tatcctgatc tgccacttca
      541 tataccatct gaaatatatt gttctcaacg taaatttcac gctttctgat agggtagcgt
      601 ttcattccga tattagggct tggtaagttt gtataacccc actctcgagc gtattgccaa
      661 atatgactga acattgaaaa ttcgttatta gcagatcccg gtgtgtcttt tcgccaagtc
      721 atatactctc ggatatgttg aggggtgatt tcttcgagag gagcgtcatt aaagaatagc
      781 agtagatttt ttatcttgca caaatctgtt tgtgctgtgc tcgctttttt ctgaggtata
      841 acttcatctc gataacgttt ggctacaagc ccgaaggtaa ttctggcagt ttggctttct
      901 ctttctatat tgagttgagc agccttaata aaagcctcat ttctatttgt gcctagagag
      961 atttccgttt ttccgtgcgt gcgatatagc caataaatga cagttttgcc tgattttcgt
     1021 gtaactgttt ttggacgtaa attctcaggt aagtagcgat tctgttgttt gcgtggtctt
     1081 cccatttttc actcccatta aataagcagg tgatttccat ttcccagtgg attgagcttc
     1141 tggatattgt tggcgggggt gcccatagac atctttgtat agcactaatg gcttatactt
     1201 gttttcatct tgaattatat agtgacgctc cctcaaccat tcgatttgtc ttttcttttg
     1261 acgatagcct gttaaatcag caagttcgtc tttcgttaaa aattcagtag gttgttccat
     1321 attctctcca aataaccccc tcatttagag ggattgagtt aaataatcgt taagcctttg
     1381 tcctatccac cgcatacaag ggacggccat tataactttt gccatatgta acccttgtgc
     1441 gttttgcgtt taccctgaat gcaaaggctg atttttgaat gtgagaaacc atctttacct
     1501 gcacttgtca ttgaggtgta ttttttgaat aatttcccgt ccagagttat tgcggcaaca
     1561 ggaactgagc aactctcaat attcattttt ttcatcctct cggactgctt gctaaaacct
     1621 ccaagtcttg agcaatgcaa catattttct gagcgtgtac accactctaa attttcaggt
     1681 cggttatttg ttttgtctct gtccttatga tttacatgta gatctggata accgtgaaaa
     1741 gtttcgcata ccagtctatg gattaaatag tcttttactc cattctcatc tacaagctga
     1801 actcttaaat aaccattctt taaaggtcta ggttttaaga ttttgttgct caagaaacta
     1861 aaaatttctc cattctcggt agctgcatag ttttcattgc gaggtaagat acgcacttaa
     1921 tctctctcct atccatttca ttacatttgt acacatcgaa tttcctatcg ccttatatcg
     1981 tgggctatca gggcattctt cggcagattt gccacggtat gtgattttcg tccagccgtc
     2041 tggaaagcct tgtagtctct cgcattcggt gggagtaagt tttcgaatag ttgttccagc
     2101 actgacagcg tgaacatcag ttcgggtaag catgtaactt acaccactct catcaaatac
     2161 gttgccgttt ccgtcgtttt gtggctgtct gccgatagta ttaccggcaa gggcaataca
     2221 agggatatta tttccacccg tccccattct tgctttcagt gttggcgttt tgtcctgaat
     2281 ttcgctaatt cgtacagctt cagcaccttt tacttcaaat aagacgttct cttgcccgtt
     2341 gtttcgacct aaacaatgag ctttgttttg gctgataatt ggatcttgcg taccgtgaac
     2401 aactagcgtc tcactcccgc cactcaaagc ctccccctga tgctttaact gtaccgcctt
     2461 tgtcagattg gcagttatgg acaacggcat tatttaagat gtagctgttg tcatctgcca
     2521 cctcaatgtt ataaacaact tcatttccag ttatctcaaa ggattgcacg cttctcaata
     2581 atctattttc atcaaggcga ctttttcttg atgtcgattg gaggaacgct cgcatttgat
     2641 aatagtcatt ctgattgaca attcttcctt ggatttcttt tgtcggagca acagttactt
     2701 tggaaacaga tgagatatat cctaaggttt gggataatgc agcaatgccg taagcaaggg
     2761 atttcgaaat gctatttgcg gtataaactc cgtttctaaa agagccatcg gtaatcatat
     2821 acccttcaaa aattttactt ctgaaagaat gagataaaca ccaagccggt atagtttttc
     2881 cgccggcttt ctcaccaaat tgcgaataaa gaaaatcagc aagttcagtg tcacaaatag
     2941 tgattttaat taccgaatta ttttcggtag taatatgatg aggaagtttg gcaaaagaaa
     3001 gagaaaactt ccgatatttc tctttattta cagaaataac aacagctttt ttatttttgc
     3061 ctttccatct tcttatccag ccatcaccaa gataataacc ggcaattaga aggacttcgt
     3121 catcagtcag tttgctatga aaaccaccat agggcataac tgaaaaatca gttaatgata
     3181 cccattgata gccctctagc ttatgagcct caatccactc aggtttggaa atgatagttt
     3241 tcctatggta ttttccattc cttttggtgt ttatcgtttg ccatttttga gcataaaaag
     3301 gatgttcagg ggtgcaaacg attgattcag gtaagccaac agccgttaat tttccaactg
     3361 gttttatttc actgccaaca cgcaaaatag gttttagttg tcctttatga gtaacaacta
     3421 aatctccgac agaaagtgtt tcgattggca tatatccttt atccgttaaa accatatgcc
     3481 cagcggtaaa gcaaggcgta cccccaacca acacatcagg agcaggaatt ttacgattga
     3541 ggattttttc aggtagggcg gtcatatcgc ccaagttcgg cacatcgggg aaacggtgag
     3601 caagcactgc acacggaaac ggctcaatct cgctgaacca taacggcgta ccgatgtcgt
     3661 gccacgctac acttgctgcc tcaatgccgg agcagataga tccataggtg aattttatta
     3721 tgttgttcat acaatcttct ccgcctgcac cgcaacgcaa atatcatcaa taaatgcgtt
     3781 aaaagtgcgg cgtaattctg gttcaaaaaa ttctgcgatc agcttggata agcgaacctc
     3841 gccgacaatt cgttcaatcg cttgctctcg ggcaaacgcc tctaattcaa gcggttttat
     3901 ctcttcttgt ggcggtggtg gcggttggat actttcccaa tgatcaggtc taattttcca
     3961 tttatcagca caaatttgtt gaatttgccc accagcacaa tcgattaact cccgagagca
     4021 atcatcggtc gttttggttt tgtctaccac caagaatagg acttcaatcg gggtatcctc
     4081 aaaggcgttt tggatgcggt tgagttctac aagttgattg ccaacttctt ctcgaaagcg
     4141 tttttctgtt tggcggtagc caacgccggg aaagagaatg aaaaatcccc attggtgact
     4201 atttgccagt ccttttagga cgaaaatatc atcaaccacc ccagattttt tccacggaaa
     4261 ttcggattga atttgtgctt gttcggcttg gcttaaatcc ttaaatttaa gggaaaatgg
     4321 cggattcata atcacgcaat cgcacggttc gccgttagga tagagaaaga agctctgatt
     4381 atagatttga gccgtaggga aattgtgccg taacacatcg cagctttcgg attggacctc
     4441 taccgcagta aaagaggttg ggcggacaaa ttgctccaac tgcccagaac ctgccgcccc
     4501 gtcaaaaaca gaaacattat ttctggcata ctgcttgacc ttttcagcaa cataacggcg
     4561 tagagcttca cctgtgatgt attcagcgaa tttgtcggct tttttgcggt tattgtgttc
     4621 ttggaattgc atttttcctc caaaaaataa ccgcttgcgg aacaagcggt tgggttaatt
     4681 agcgttgagc tgtacaaatt ttgccatcgc agtcgttgcg taactctaga tggtcagcag
     4741 caactgcaaa ggcggttaaa aagagtatga tttttgcgta tttcatcgtc taatcctcaa
     4801 aatttgggtg caggaaaccg ccgcttgatg tttcgcaagc ggtcgggttt tataattttt
     4861 tgtgattaga acgttggctt tttaaatgcc gggtcgaaac ctcgaacgtg tttgagtact
     4921 cgccagtttg tcatcgggtc gtaatcaaat tctttggtaa tccgttcaag aattttatga
     4981 gccgattgtg cggtgtggct atattcaaaa ccctgtccgt agatttcagg tgcgatattt
     5041 gagccgagtt tttgaaacat cgggtagata tagcggaaag tgtgaatgcc acgcacgaaa
     5101 gcgaaccaag cccatatgag gctttggagt tcatcttcgg taaagtcgaa agagtatttc
     5161 tttggcggtg gtgcaagcgg taggttttgc tgattatttt gcaacgcata tttaccagtc
     5221 ttgcgaattt gtggcagaac ttcttcaaat acccacgctt caaacttttc cgcttctggt
     5281 ttgcgtgatt tgataatcag tcggtatagg tttggttcgt tgatgaagat cgctttttgt
     5341 tgtctctgca ttttatcggt gaggtatcgt ttcgataccc cattttcttt acagtgtttt
     5401 tgcagagtaa ggctttcatt tacataacct aaaattcggc aaacatctgc accgcaaaac
     5461 cacggttctt gatttggatc gataatgaca cgaacagcag atgaattgaa attgaaaaca
     5521 gggaattgaa cttgatttga cattttttgt accttttgga tttaagtatt taatccgatc
     5581 attaagtggg tgatcggggt tcaacttacc ctccaaaaga tgggcggagc ttattcagat
     5641 gtattgtata tcgctctctc gccccgatca tttcggatta aacctaaaat ttaggtacaa
     5701 aaaaaccgct ttatatcgga ggcggaaacc gcttttggaa agtaagtgtg gttatcttag
     5761 ccgaaagggc ggttttttgt caattagatt ttattcttca attagcaatg gcttttcttt
     5821 cagttcttca atcgctctgg tttgtaacag cgagctcatt tgctgaatag ccaaacggtt
     5881 gagcattgcc agtcgctctt cttggctgta accttgctca attagtaggg cgttttggct
     5941 ttccaatgca gcaaggacgg taagttgctc aacggtagcg tggtctcgca tattgccttt
     6001 caggttcgga ttgcggtctt tccactgttt tgccgtttgt ccgaagagag cttgattgag
     6061 aatgtccgct tcgctggaat aaacgaaagt atgttgtttt gtattcagca atgccggtat
     6121 gatgtgagct ttgatggcat cagtatgaat gcggtagttc gccttgctta aaatgcgttt
     6181 tacgctccat tcgagcttac tttcgttcgc ttcttgctgt ttgagtcgtt ggaactcttt
     6241 gatgagataa agcttaaatt ctggactgag ccaagaacca aattcgagag caatatcttt
     6301 gtgggcgtaa gtgccgctac catatcgtcc tgcttttgct actagcccaa tagctcccgt
     6361 tgcagtccat tttttgacag acatcacaaa gcggtttaag ccgatgttgt ttttaatttg
     6421 gtgtaattcc accaaattaa aatttggatt attcagctct tcccaaactt gaagaaattc
     6481 aatggtattt ttattttgaa gccagttttt aatgagctgg tcactgtctc caaaagcttt
     6541 gcacatatca gttaggctga tgtaatcctc accatcacga gcggttactt tgacctccac
     6601 gccttgtaca atcattacgc tatttgccat ttcgtttctc cgtttttagg tacaaaaaaa
     6661 gccgttggtg gacggcttga aagtgcggtt atcttaatcc gaattggggg cggtgtcaat
     6721 tagattaatc cgtataagta tggaaccaag ttaaagagta gaactacaac acataaccaa
     6781 aaaatgaaat tcatatcttg ctcagttttg attttgtgta aaatacgctt aagtttgttt
     6841 ttcatttagg aaacctttaa tgtttagttt attgcgtgat gctttaacct ttgctgcaat
     6901 gtttacaggg attttatttc ctgcaattat tcataaatac gatctatcac ttggtacaca
     6961 aatccaatta cttagctttg ttccagcttt ctttgtgttg tgccattttc ttattaagcg
     7021 aaaatatcaa cgtgattttg acagactttc gtatttatct gctgtggttt gcgtagcaat
     7081 gtgtgcaggc gttggagcat ttagccaatc agaaatgatt gagctaggtt ttaaagttga
     7141 gcgtgttctt gattatgtga ctatcaaatt cttactctac ttttggagct tgctgttatt
     7201 gccagttgtg ctaagaaaat tgtttgggaa agattagttg gttatttaaa gagagctatt
     7261 tgtcaaacct caatatctat ttttgcgatc tgcatcgaaa aaataaattt tcgttctgac
     7321 agtaaaacgc tttcgtagta atctcataaa aaacataagg
//
LOCUS       DANLXS010000117         7359 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.117, whole genome shotgun sequence.
ACCESSION   DANLXS010000117 DANLXS010000000
VERSION     DANLXS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.117"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            124..792
                     /locus_tag="PXD71_001835"
     CDS             124..792
                     /locus_tag="PXD71_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6291925.1"
                     /translation="MAHNGSGKSTLMRLLSGDITPTSGSILVDGLPLHQLSTKEAAKH
                     IAYLPQRLPEAADFLVQELVALGRFPYQKWLQKPTEFDVQIVEQAMESTSVTQFAKQP
                     VNSLSGGEKQRVWLAMCLAQQTPYLLLDEPLAALDIVYQVEVMQLIRKLVNEQGLTVI
                     IIIHDINLASQFCDEIIALKQGELCIQGSVDEIMQEQKLLEIFGLKLHLFNHPAGLHK
                     VAVL"
     gene            789..1670
                     /locus_tag="PXD71_001836"
     CDS             789..1670
                     /locus_tag="PXD71_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6291926.1"
                     /translation="MMFRQYKRFIFLFILQISTAFAQPNIFTPDWSIASALTEMGNPP
                     IAMGDKRIYPLWSHRPIQPNSVLDIGARYQPNRELLAQLPVELVLSNFFYAHLASVYP
                     KEIPQIDVLFDGGNTEPQQHWHTYQKATRMIGTEIKNPNAAEAYLTKTEQTLAQWGKE
                     ILNSNPDIDYYSVVQFGSPRELRIYASNSMFRVAFDLMGLKQADLGVGDRWGNKLIPV
                     SDLAKLPERTCLIVISPFSKMTQADLENSYIWQRLGFGKNRCMKVLPPVWLFGGADSI
                     SHFAEFLHNAMTQGEKE"
     gene            1670..2485
                     /locus_tag="PXD71_001837"
     CDS             1670..2485
                     /locus_tag="PXD71_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL6291927.1"
                     /translation="MKSSFLLIGCILLSLVTAVIRLNMVWEWEWQLLLINVDLLPINQ
                     AAVKAALLPEMTTSFLAGGLLAIASSGLQHIVRNNLASDSTLAVSSGAQLALMIAVIF
                     FPAAELFSSFWIALLGSLAAIVLVLFIAKASKLNPLTLILGGLIVNILFGAIASLLTL
                     FYFDFLFGVMVWGSGSLLQDGWATSITLAITVVVAFFIFVLLSRPLTILSLDDEQARR
                     LGAPVNLLRYLVIFVCAAITALVVSKIGVIGFIGFAVQVWLILPKYDIYYFAS"
     gene            2482..3618
                     /locus_tag="PXD71_001838"
     CDS             2482..3618
                     /locus_tag="PXD71_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6291928.1"
                     /translation="MIAFLAGGFLLFFTNNLIGIVSRIYDTLLPVGAFTATLGAPLLI
                     FLVLRQRKAQFEASNTAYFPVKRLNSWKLPILLLCAGVILFCLLQIIAPTMNGWAISI
                     DENLMSSYRLPRSLSAIATGAMLAVSGSLLQILTRNPMASPEVLGVSSGATIAAVLSF
                     LLFPTLSSISFLLFGVLGSLTVLALIMWLSRKLQPSALMITGVAIGALVSGLMAIIQL
                     SGSPRLTAVISWLSGSTYYANPNTVWILLSVAILLISATLLFIKPLRLISLGETVSYH
                     LGLEVKKVQMIVLLLIALMSAASTLAVGPMSFIGLMTPHLAKNLGAITPEKQIPISAL
                     LGAMLMLIADWLGRYLIFPYEIGAGIIASILGGIYFLLLLKQHK"
     gene            3646..4044
                     /locus_tag="PXD71_001839"
     CDS             3646..4044
                     /locus_tag="PXD71_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL6291929.1"
                     /translation="MNTLSKAIKLALYSSGIGFISTAYAETVTHLDTVEVSAETPNAS
                     SEETGRYAQKKAKGSNGMALSAKDTPQSVTAVTHQQIRDQNLNTIAKALEATHGVSVS
                     LLDRGRYSFSARGFGIDKVKVDGMDLKVSK"
     gene            4079..5782
                     /locus_tag="PXD71_001840"
     CDS             4079..5782
                     /locus_tag="PXD71_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL6291930.1"
                     /translation="MLYDRVEVVRGATGLTTGAGDPSASVNLVRKRANSISRYTILEA
                     GIGRYADFNTMIDHSQALNNSGSVRGRFIAEHKNGNTFIKNEKERYTTLYGNIEADLT
                     DTTKLGLGVSYQKEKRDAALWGGLPAFYSDGGETNWSRSKNASPEWAYWDNESVNYFA
                     DITQKLGEKWELALKGNYRVGKHNSELFYFSGSSLNRQDGLGWSPWPGKFKNDNTQSN
                     VQLDLTGSFSAWGLEHDAAIGIQYNRAHRNSYAADNSTFDGAFDFNNWNGIYARPNWG
                     SMSEKYYQNLKEFGVYVSTRLHLTDRLSMLLGSRISSYRTEGTFYGSTQNFHANDVWT
                     PYAGLTFDINPQNTVYVSYTDIFKPQSQVDINNKLLSPVTGETYEAGWKGSYFNDNLQ
                     TQISVFETRQDNLAQSAGINPNTLLAYYTASKGAKVRGFEVEASGNVTEKLKLSAGYT
                     QWKGDDASGNPINTTQPRKQFKLFATYDMNRLVQGLTVGAGVNWQSRIYTKHATYGEY
                     GQKAYTLVNLMARYQFNDNFSAQLNIDNLFDKKYRNALSFSQYSYGEPFYTRLNLRYE
                     F"
     gene            5894..6538
                     /gene="adk"
                     /locus_tag="PXD71_001841"
     CDS             5894..6538
                     /gene="adk"
                     /locus_tag="PXD71_001841"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL6291931.1"
                     /translation="MKVILLGAPGAGKGTQAQFIMNKFGIPQISTGDMFRAAIKESTE
                     LGKQAKALMDEGKLVPDELTVALVKDRIAQPDCANGFLLDGFPRTIPQADALKESGVK
                     IDLVLEFDVADEVIVERMSGRRVHQPSGRTYHVVYNPPKVEGKDDVTGEDLIIRQDDK
                     PETVLERLAIYHKQTKPLIAYYTAEAEAGNTRYERLDGTKPVEEVSAELAKILS"
     gene            complement(6600..>7359)
                     /locus_tag="PXD71_001842"
     CDS             complement(6600..>7359)
                     /locus_tag="PXD71_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL6291932.1"
                     /translation="ANWTKINDVNYTWGPFNIYHITLFTESGTYVEGTRPLMLTLQYK
                     KPWEGRDFAVSLARTWNNLGIKLPEKELDEVITHLRKTFPDIKPEDTLHYIALEDRGY
                     FILNDKVVSDVFNKAFNDAIVAIWLDPKMDISHQLLDPSYKPRAEAESIKPQVEERVA
                     ENLQEPIVNTEVSKELKADSQAVEQAPNKIDIPVADKTQASVVNEATQPVENKDKKEP
                     EKLEEKPKEMKIENPEVFISPIYDDILMLTKYCV"
BASE COUNT         2151 a         1457 c         1461 g         2290 t
ORIGIN      
        1 ccaccttttt tatgctctta aaacacaaca ctatgattca aataaattcc attagctact
       61 ctgttgaaca aaaccgtaaa ctgctaagca atatttccct caatttaagc aaggggcgtt
      121 tatatggcgc ataacggctc gggtaaatca acgttaatgc gtttactaag cggagatata
      181 acgcctacca gcggttcaat tttagtcgat ggattaccac ttcatcagct ttctaccaaa
      241 gaagcggcaa aacatattgc ctatttacca caacgtttac ctgaagcggc ggatttctta
      301 gtgcaggaac tggttgcttt agggcgattt ccatatcaaa aatggctaca aaaaccaaca
      361 gaatttgatg ttcaaattgt tgagcaagca atggaaagca cctccgttac tcagtttgct
      421 aaacagcctg taaatagtct atcaggcgga gaaaaacaac gagtttggct tgctatgtgc
      481 ttagcccaac aaacacccta tttactgcta gatgagccat tggctgccct tgatattgtt
      541 tatcaagttg aagtaatgca gcttattcgc aagctagtga atgaacaagg tctaactgtc
      601 attatcatta ttcacgatat taatcttgcc tcccaatttt gtgatgaaat tattgcgtta
      661 aagcaaggcg aactctgtat tcaaggttct gttgatgaaa ttatgcaaga acagaaatta
      721 cttgagatct tcgggctaaa actgcacttg tttaaccacc ctgcaggctt acataaagtg
      781 gcggtattat gatgtttagg caatacaagc ggttcatttt tctctttatt ttgcaaattt
      841 ctaccgcatt tgctcaacct aatattttta caccagattg gtctattgcc tctgccctta
      901 ctgaaatggg aaatccgccc attgcgatgg gggataagcg aatttatccg ctatggtcgc
      961 accgccctat tcagcctaat agcgtgttag atattggagc gcgttatcag cctaatcgcg
     1021 aattattagc acagcttcct gttgaattgg tgctatcaaa ctttttctac gctcacttgg
     1081 cttctgttta cccgaaagaa attccacaaa tagacgtgct atttgatggt ggcaatactg
     1141 aacctcagca acattggcat acttaccaaa aagccactcg tatgattgga acagagatta
     1201 aaaaccctaa tgcggcagaa gcatatttaa cgaaaactga acaaacgttg gctcaatggg
     1261 ggaaagaaat ccttaacagt aatccagata ttgattacta ctctgttgtt caatttggct
     1321 ctccgagaga gttacgcata tatgcgtcta attcaatgtt ccgtgtagcc tttgatttga
     1381 tgggcttaaa acaagctgat ttaggtgtgg gagatcgctg gggaaataaa cttattcctg
     1441 tgagtgattt agctaaatta cccgaacgca cctgtttaat tgtcatctca cctttttcta
     1501 aaatgacgca agcagattta gaaaatagct acatttggca acgtttagga tttggtaaaa
     1561 accgttgtat gaaagtatta ccgcctgttt ggctctttgg tggggctgat tccatctcgc
     1621 actttgctga gtttttacac aatgcaatga cacaaggaga aaaagagtaa tgaaatcatc
     1681 attcttattg attggctgta ttcttttatc cttagtaaca gctgttatcc gtttaaatat
     1741 ggtctgggag tgggaatggc agttactact cataaatgtt gatttattgc caattaatca
     1801 agctgcggta aaagcagctc ttttacctga aatgacaacg tcatttttag caggcggttt
     1861 attggctata gccagcagtg gcttacaaca tattgtcaga aataatttag cgtcagatag
     1921 cacacttgcc gtcagtagcg gggctcaact tgcattgatg attgcggtta tctttttccc
     1981 agccgccgag cttttttcta gtttctggat tgcgttactt ggctctttag ctgctatcgt
     2041 actggtttta tttattgcta aagcctctaa gctcaatccc cttaccctta ttttaggggg
     2101 attgattgtt aatattttgt ttggagctat tgcctcttta ctgacccttt tctactttga
     2161 ttttttattt ggcgtgatgg tttggggaag tggctcactt ttacaagatg gttgggcaac
     2221 ttctattacc ttagcgataa cggtagtagt tgcatttttt atctttgtct tactctctcg
     2281 tccactcaca attttaagtt tagatgatga acaagctcgc cgcttaggcg cgcctgttaa
     2341 tttactacgc tacttagtta tttttgtttg tgccgccatt acagcattgg ttgtaagcaa
     2401 aataggtgtg attggcttta ttggatttgc agtgcaagtt tggctcattt tgccaaaata
     2461 cgacatttat tacttcgcct cttgattgct tttctagcgg gcggattttt actctttttt
     2521 accaataatc tcatcggcat tgttagcaga atttacgaca ctctactacc tgttggtgca
     2581 tttaccgcaa cactaggcgc accattatta attttcttgg tattacgcca acgtaaagca
     2641 caatttgaag caagcaatac agcatacttc cccgttaaac gcctcaattc gtggaaactg
     2701 ccaatattgt tgttatgcgc tggagttatc ctattttgtc tattacaaat cattgctcca
     2761 acaatgaatg gctgggctat tagcattgat gaaaacctga tgagttccta ccgcttgcct
     2821 cgtagtctaa gtgcgatagc aacaggggca atgcttgcgg taagtggtag cctattacaa
     2881 atcttaacga gaaacccaat ggcaagccct gaagtattag gtgtgagttc aggggcaact
     2941 attgctgcag tactgtcatt cttacttttc ccaacattat ctagcattag cttcttgtta
     3001 tttggtgtat tagggagttt gactgttctc gctcttatta tgtggctctc tcgtaaatta
     3061 caaccatcag cgttaatgat tacaggtgtt gctattggag ctttagtcag tggcttaatg
     3121 gctattattc aactaagcgg cagccctcgt ttaacggcag ttattagttg gctgtctggc
     3181 tcaacctatt acgctaatcc gaatacagtt tggattttat tatctgttgc aatcctactt
     3241 atcagtgcca ctttactgtt tatcaaacct ttacgtttga tctcacttgg cgagaccgtt
     3301 tcttaccatt tagggttaga ggttaaaaaa gtacaaatga ttgtattact tttaattgcc
     3361 ctaatgagtg ccgcctctac acttgcggta gggccaatga gcttcattgg gctaatgacc
     3421 cctcatttag ctaaaaattt aggtgcaatt acaccggaga aacagatccc tatatcggca
     3481 ttattagggg caatgctaat gctcattgcc gattggctcg ggcgttatct aattttccct
     3541 tatgagattg gggcaggcat tattgcctct attctcgggg gtatctattt cttactgtta
     3601 ctcaaacaac ataaataatt ttttattcat tacttaggag aaattatgaa cacgctatct
     3661 aaagcgataa aacttgcctt atacagttct ggtatagggt ttatctcaac agcttacgct
     3721 gaaacagtta ctcatcttga taccgtagaa gtaagtgccg aaacgccaaa tgcctcatcg
     3781 gaagaaactg gacgttatgc acagaaaaaa gccaaaggct ctaatggaat ggcattgagt
     3841 gcgaaagaca cgccacaaag cgtaacggct gtaacgcatc aacaaatcag agatcaaaat
     3901 cttaatacga ttgccaaagc acttgaagca actcacggtg tttctgtttc tttacttgac
     3961 agaggacgct attctttcag tgctcgtggt tttgggattg ataaagtcaa ggtagatggt
     4021 atggatctca aagtaagtaa ataaccaatg gacaacgggc gaaaatacag gcaacagcgt
     4081 gttatacgac cgagtagaag ttgtgcgtgg tgctacaggc ttaactactg gggcaggcga
     4141 tccttctgca agtgttaatc ttgttcgtaa acgagccaat agcatttccc gttatactat
     4201 tttagaagct ggaatcgggc gttatgctga tttcaataca atgattgacc atagccaagc
     4261 attgaataat tcaggatcag taagaggccg ctttattgca gagcataaaa acggtaatac
     4321 gttcattaaa aatgaaaaag aacgttacac cacgctttat ggcaatattg aagcagactt
     4381 aactgacact accaaacttg ggttaggtgt aagctatcaa aaagaaaaac gtgatgcagc
     4441 actttggggt ggtttacccg cattctatag cgacggtggt gaaactaact ggagcagaag
     4501 caaaaatgcc agtccggaat gggcttactg ggataatgaa tccgtaaact attttgccga
     4561 tattacacaa aaactaggcg aaaaatggga acttgcatta aaaggcaatt accgtgtagg
     4621 caaacataat tcagaacttt tctatttcag cggctcatca cttaatcgcc aagatggatt
     4681 aggttggtcg ccttggccgg gtaaatttaa aaatgataat acacaatcta atgtgcagct
     4741 tgatcttacc ggttcattct ctgcttgggg attagaacac gatgcggcaa tcggtattca
     4801 atacaatcga gcacatcgta attcctacgc tgccgataat agcacctttg acggagcttt
     4861 cgactttaat aattggaatg gcatttatgc tagaccaaac tggggaagta tgtctgaaaa
     4921 atactaccaa aacttaaaag agttcggggt gtatgtttct acccgtttac atcttacaga
     4981 tagactttct atgcttctag gttctcgtat ttcatcgtac cgcacagaag gcactttcta
     5041 tggttcaaca caaaacttcc acgctaacga tgtttggaca ccttatgcag gattaacctt
     5101 tgatattaat ccacaaaata ccgtttatgt aagttatacc gatattttca aaccacaaag
     5161 tcaagtggac atcaataaca aactactctc tcctgttacg ggcgaaacct acgaagcagg
     5221 ttggaaaggg agttatttca atgacaactt gcaaactcaa attagcgtat ttgaaacaag
     5281 acaagataat ctagctcaat ctgccggtat taaccctaat acattattag cctactacac
     5341 agcctctaaa ggtgcgaaag ttcgaggctt tgaagtagaa gcatctggaa atgtaactga
     5401 aaaattaaaa ctcagtgcag gatatactca gtggaaaggc gatgatgctt caggtaaccc
     5461 aattaacaca actcaaccac gcaaacaatt taaactcttt gcaacttacg atatgaaccg
     5521 tttagtgcaa ggtttaacgg taggagcagg tgtaaactgg caaagcagaa tttataccaa
     5581 acacgctact tatggcgaat atggtcaaaa agcttacact ttagtgaatt tgatggctcg
     5641 ttatcaattt aatgacaact tctcagcaca gcttaacatt gataacttat tcgataaaaa
     5701 atatcgtaac gccctcagct ttagccaata tagctacggt gagccattct atacacgctt
     5761 aaatttacgc tatgaattct aattccgtat aacaagcggt caaaaaatta aaaaaatttg
     5821 caattttagg cagtaatcgg taaggttact gcctaagtga ttttattatt tttagtttaa
     5881 ttgaggtttt caaatgaaag tgattttatt aggtgcgccg ggtgcgggca aaggtacgca
     5941 agctcagttt attatgaaca aattcggtat tccgcaaatt tcaaccggcg atatgttccg
     6001 tgcagcgatt aaagaaagca ctgagcttgg taaacaagca aaagcattga tggacgaagg
     6061 caagttagtg ccggatgaat taacggtcgc cttagtaaaa gatcgtattg ctcaaccgga
     6121 ttgtgctaat ggcttcttat tagacggttt cccacgcacc attccgcaag ctgatgcgtt
     6181 aaaagagtca ggtgtaaaaa ttgatttagt gttagagttt gatgtagctg atgaagtcat
     6241 tgttgagcgt atgagtggtc gtcgtgttca ccaaccttca gggcgtactt accacgtggt
     6301 gtacaatcca ccgaaagtgg aaggcaaaga tgatgtgact ggtgaggatt taattatccg
     6361 ccaagatgac aaacctgaaa cggtattaga gcgtttagcg atttaccaca aacaaaccaa
     6421 accgttaatc gcctactaca ccgcagaagc agaagccggc aacactcgtt atgagcgttt
     6481 agatggaaca aaaccagtgg aagaagtaag tgctgagtta gcgaaaattt tatcttaaat
     6541 ggattattaa aatgaacaag cggtaatatt tttcgagaat attaccgctt gttctttgct
     6601 taaacacaat atttggtgag cattaagata tcatcataaa tcggtgaaat aaacacttcc
     6661 ggattttcaa tcttcatctc ctttggtttt tcttctagct tttctggctc tttcttatct
     6721 ttattctcta ccggttgagt cgcttcattt accacactag cttgagtttt atctgctaca
     6781 ggtatatcta ttttgtttgg tgcttgctct actgcttgag aatccgcttt cagctcttta
     6841 gaaacttcag tatttacgat aggttcttgt aagttttccg ctactctttc ctctacttgt
     6901 ggcttaatac tttctgcctc tgctctcggt ttataagatg gatcaagtaa ttgatggcta
     6961 atatccattt tcggatcaag ccaaattgct acaatagcat cattgaaagc tttattaaac
     7021 acatcagaga ctactttatc atttaaaata aaataacctc tatcttctaa tgcaatatag
     7081 tgcaatgtat cttcaggctt aatatctggg aaagtttttc tcaggtgggt aataacctca
     7141 tctaactctt tttcaggtag cttaatgcct aaattattcc aagttcttgc taaagaaacc
     7201 gcaaaatcac gcccctccca cggtttttta tattgtagag taagcattaa aggacgtgta
     7261 ccttctacat aagtaccgct ctcagtaaaa agcgtaatat ggtaaatatt aaatggcccc
     7321 cacgtataat tcacatcatt aatttttgtc caatttgca
//
LOCUS       DANLXS010000118         7337 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.118, whole genome shotgun sequence.
ACCESSION   DANLXS010000118 DANLXS010000000
VERSION     DANLXS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.118"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            37..456
                     /locus_tag="PXD71_001843"
     CDS             37..456
                     /locus_tag="PXD71_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor domain-containing protein"
                     /protein_id="HDL6291933.1"
                     /translation="MVPSNEAVLKAVHDTLVISTTDLQGVITYANDLFCKLTGFTREE
                     LIGQPHNIVRHPAVPKEVYKDMWDTIQAGKIWTGIVPNCGKGGVVYVVDTTVQPMFDE
                     TGKITGYISVRRVINDLMTNFEAVESAKEIFDDFYGE"
     gene            527..2083
                     /locus_tag="PXD71_001844"
     CDS             527..2083
                     /locus_tag="PXD71_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HDL6291934.1"
                     /translation="MSLIPHIHIAYGSESGNAEKLAQQLAIQPFLLPYSPTLSTLNET
                     DLSQLSAESLLLIVTSSFGDGEPPENADEFAEKLENLTACNLQYAIFGLGDITYDKFC
                     GYSKQLDSLLQQKQAKAVIARVDADLNYQEIFKQWLPLVEQALHSTNKETIQHSLSVQ
                     AYGEEMAYQAEVLEIKHLANSSPAVYHIRLSLKESGIFYQAGDLIYVKVQQPDRLLNE
                     YAEWFNDQNAVALLRHKELRLLSKNVLRDIQKICGSNELKDLTKISNKKALEQYVYGR
                     DLLDVLRDFDPNKSVTLADLEPILSNLAARAYSISSCGKTHPDYVDLCIRHVSYHLNG
                     RDYQGTASGYLSELQAGDFVSIFANANPNFRLPETLTAPVVMIGSGTGIAPHIAFLQA
                     LEQQYQGVESYLFFGERYRDKDFLYQTELENYLAKGTLTQLFTAFSRDQAEKFYVQDA
                     LSQQAELVWHLIQQGAYFYICGSKAMSKAIDAELIKIADQIGGDPYIDDFNNIIAKLV
                     AEGRLMRDVY"
     gene            complement(2141..2398)
                     /gene="rpsQ"
                     /locus_tag="PXD71_001845"
     CDS             complement(2141..2398)
                     /gene="rpsQ"
                     /locus_tag="PXD71_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL6291935.1"
                     /translation="MTDKIRTVQGKVVSDKMDKSFVVAIERTVKHPLYGKFIRRTTKL
                     HVHDENNEAKLGDVVEIKECRPISKTKSWTLVRVVEKAVVA"
     gene            complement(2398..2589)
                     /gene="rpmC"
                     /locus_tag="PXD71_001846"
     CDS             complement(2398..2589)
                     /gene="rpmC"
                     /locus_tag="PXD71_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL6291936.1"
                     /translation="MKAQELRNKSVEELNGELVNLLGEQFKLRMQAATGQLQQTHQLK
                     QVRRSIAQVKTVLTEKAGE"
     gene            complement(2589..2999)
                     /gene="rplP"
                     /locus_tag="PXD71_001847"
     CDS             complement(2589..2999)
                     /gene="rplP"
                     /locus_tag="PXD71_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL6291937.1"
                     /translation="MLQPKRTKFRKVHKGRNRGIAGGTEVSFGSFGLKAIGRGRLTAR
                     QIEAARRAMSRAVKRQGKIWIRVFPDKPITEKPLEVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVSEEVARNAFALAAAKLPFKTTFVTKTVM"
     gene            complement(3013..3720)
                     /gene="rpsC"
                     /locus_tag="PXD71_001848"
     CDS             complement(3013..3720)
                     /gene="rpsC"
                     /locus_tag="PXD71_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL6291938.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTQDFADNLDGDFKVRKFLN
                     KELANASVSRITIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRNAVAKIAGVPAQIN
                     IAEVKKPELDAKLVADSIASQLERRVMFRRAMKKAVQNAMKLGAKGIKVEVSGRLGGA
                     EIARSEWYREGRVPLHTLRADIDYSTAEAHTTYGVIGVKVWIFKGEILGGMAAVIESE
                     KEPAAQPKKAPRGKGRK"
     gene            complement(3740..4072)
                     /gene="rplV"
                     /locus_tag="PXD71_001849"
     CDS             complement(3740..4072)
                     /gene="rplV"
                     /locus_tag="PXD71_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL6291939.1"
                     /translation="METIAKHRYARTSAQKARLVADLIRGKKVSVALEILTFTNKKAA
                     TLVKKVLESAIANAEHNDGADVDDLKVAKIFVDEGPSMKRVMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(4083..4358)
                     /gene="rpsS"
                     /locus_tag="PXD71_001850"
     CDS             complement(4083..4358)
                     /gene="rpsS"
                     /locus_tag="PXD71_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL6291940.1"
                     /translation="MPRSLKKGPFLDLHLLKKVEKAVESGDKKPIKTWSRRSMIIPSM
                     IGLTIAVHNGRQHVPVYVSDEMIGHKLGEFAPTRTYRGHAADKKAKK"
     gene            complement(4389..5210)
                     /gene="rplB"
                     /locus_tag="PXD71_001851"
     CDS             complement(4389..5210)
                     /gene="rplB"
                     /locus_tag="PXD71_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL6291941.1"
                     /translation="MAIVKCKPTSAGRRHVVKVVNAELHKGKPFAALLDTKSKTGGRN
                     NLGRITTRHIGGGHKQHYRLIDFKRNKLDIPGVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLTAGDQIQAGASAPIKVGNALPMRNIPVGTTVHNVELKPGKGGQIARSAGA
                     YVQIIAREGNYVTLRLRSGEMRKVLAECTATIGEVGNSEHMLRVLGKAGANRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRHNKRTDKFIVRRRGK"
     gene            complement(5229..5534)
                     /gene="rplW"
                     /locus_tag="PXD71_001852"
     CDS             complement(5229..5534)
                     /gene="rplW"
                     /locus_tag="PXD71_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL6291942.1"
                     /translation="MIQQERLLNVLKAPHISEKATNNAEKGNTIVFKVALDANKVEIA
                     NAVEELFEVKVDSVRTVVVKGKTKRRGAKVGRRSDWKKAYVTLQEGQSLDFVEGAAE"
     gene            complement(5531..6133)
                     /gene="rplD"
                     /locus_tag="PXD71_001853"
     CDS             complement(5531..6133)
                     /gene="rplD"
                     /locus_tag="PXD71_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL6291943.1"
                     /translation="MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGSRA
                     QKTRAEVSGSGKKPWRQKGTGRARSGDIKSPIWRSGSVTFAAKPQDHSQKVNKRMYRG
                     AIKSILSELVRQDRLVVVEKFEIDAPKTKVLAQKLKDMALNDALIITANVDENLFLAA
                     RNLYKVDVRDVQGIDPVSLIAFDKVVVTVDAVKQIEEMLA"
     gene            complement(6149..6778)
                     /gene="rplC"
                     /locus_tag="PXD71_001854"
     CDS             complement(6149..6778)
                     /gene="rplC"
                     /locus_tag="PXD71_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL6291944.1"
                     /translation="MIGLIGRKVGMTRVFTEDGVSIPVTVIEIEANRVTQVKTLENDG
                     YSAIQVTTGSKKASRVTKPEAGHFVKAGVEAGRGLWEFRTTEGEEFTLGQEINVDIFT
                     DVKKVDVTGTSKGKGFQGGVKRWNFRTQDATHGNSLSHRVLGSIGQNQTPGRVFKGKK
                     MAGHLGAERVTVQSLEVVRVDVERKLLLVKGAVPGATNGDVIVKPAVKA"
     gene            complement(6804..7115)
                     /gene="rpsJ"
                     /locus_tag="PXD71_001855"
     CDS             complement(6804..7115)
                     /gene="rpsJ"
                     /locus_tag="PXD71_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL6291945.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQSTAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHKRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
BASE COUNT         2132 a         1563 c         1362 g         2280 t
ORIGIN      
        1 catttatatt aatggagatt caacacggct gaacaaattg ttccttcaaa tgaagctgtt
       61 ttaaaagcag tccacgatac tttagttatt tcaacaactg atttacaagg tgttattact
      121 tacgctaacg accttttctg caaattaacc ggttttaccc gtgaagaatt aattggacaa
      181 ccgcataata tcgtacgcca cccagctgta ccaaaagaag tttataaaga tatgtgggat
      241 acaatccaag ccggtaaaat ctggacaggt atcgtaccaa actgcggtaa aggcggtgta
      301 gtttatgtgg ttgataccac cgttcaacca atgtttgatg aaacaggcaa aattacaggc
      361 tatatcagcg ttcgccgtgt aattaatgat ttaatgacca actttgaagc agtagaatca
      421 gcaaaagaaa tctttgatga cttctacggt gaataattaa aaaacaatga ttgcgtgtaa
      481 aagatgatct cttttacacg caatttttta ttttcgagga gtttgtatgt ctctcattcc
      541 ccatatccac attgcttatg gttcggagtc tggtaacgca gaaaagcttg cccaacaatt
      601 agctatccaa ccctttttac ttccctactc acccacactt tctacattaa acgaaaccga
      661 tttaagccaa ttatctgccg agagtttatt gcttattgta accagctcct ttggtgatgg
      721 cgaaccacct gaaaacgccg atgaatttgc agaaaaattg gaaaatttga ccgcttgtaa
      781 tctgcaatat gcgatattcg ggctaggtga tattacttat gataaattct gtggctatag
      841 caaacaacta gattcattat tacaacaaaa acaagctaaa gctgtgattg caagggttga
      901 tgccgattta aactatcaag aaatttttaa acaatggcta cccctagtgg agcaggcatt
      961 acattcaaca aacaaagaaa cgatccaaca ttccctttct gttcaagctt acggcgagga
     1021 aatggcatac caagcagaag tgttggaaat taagcatttg gcaaactcaa gccctgcggt
     1081 gtatcacatt cgtttatcat taaaagagag cggtattttc tatcaagcag gggatttaat
     1141 ttatgtcaaa gtgcagcaac cagacagact attaaatgaa tatgcagaat ggtttaacga
     1201 tcaaaatgca gttgcattac tacgccataa agaactccga ctactaagta aaaatgtact
     1261 gcgtgatatt caaaaaattt gcggcagtaa tgaactcaaa gatttaacta aaatttccaa
     1321 taaaaaagca ttggaacaat atgtctatgg gcgggattta ttagacgtat tacgcgattt
     1381 tgacccaaac aaaagcgtga cattagccga tttagagcca attttatcta acttagccgc
     1441 acgagcctat tcaattagct cttgcggtaa aactcaccct gattatgttg atttatgtat
     1501 ccgccacgtt tcgtatcatt taaacggtag agattatcaa gggactgcaa gtgggtattt
     1561 atccgaatta caagcgggcg attttgtctc gatttttgca aatgccaatc cgaatttccg
     1621 tttacccgaa acattaaccg ctccggtggt gatgattggc tcaggtactg gcattgctcc
     1681 gcatatcgca tttttacaag cgttagagca gcaatatcag ggggttgaaa gctatttatt
     1741 cttcggcgag cgttatcgtg ataaagattt tctgtatcaa acggaactgg aaaattactt
     1801 agcaaaaggc accttaactc agctgtttac tgctttttca cgtgatcagg cagagaagtt
     1861 ttatgtgcaa gatgccctat cgcaacaagc agagttagtt tggcatttaa ttcagcaagg
     1921 ggcttatttc tatatttgtg gtagcaaagc aatgagtaaa gcaatagatg cggaactcat
     1981 caaaattgca gatcaaattg gaggcgatcc ttatattgat gatttcaata atattattgc
     2041 aaaattggtc gcagaaggcc gattaatgcg agatgtatat tagcaaatga aaaaaactaa
     2101 aaacccagca aaagctgggt ttttatttaa tcatagttca ttaagcaaca actgctttct
     2161 caactacacg aactaaagtc caagatttag tttttgaaat aggacgacat tccttaattt
     2221 caacaacatc acctaattta gcttcattgt tttcatcgtg tacatgaagt ttagtagtac
     2281 gacggataaa cttaccgtat aacggatgtt taactgtacg ttcgatagca acaacaaatg
     2341 atttatccat tttgtcgcta accactttac cttgtactgt acgaatttta tcagtcatta
     2401 ttcacccgcc ttttcggtta atacagtttt aacttgtgca atactacgac gcacttgttt
     2461 taactggtgg gtttgttgaa gctgaccggt ggctgcttgc atacgcaatt tgaattgttc
     2521 acctaaaagg ttcactaact caccattcag ctcttcaaca cttttattac gtagttcttg
     2581 agctttcatt acatcaccgt cttagttaca aatgtggttt tgaacggaag tttagctgcc
     2641 gctaatgcaa acgcattgcg tgcaacttct tcagaaacac catccatttc atagagaact
     2701 ttacccggtt ggattaaggc tacccagtac tcaacgttac ctttaccttt acccatacgg
     2761 acttctaatg gtttttcagt aattggtttg tctgggaaca cacggatcca gattttacct
     2821 tgacgtttaa ccgcacggct catcgcacga cgagcagctt caatttgacg agctgttaaa
     2881 cgaccacggc caatagcttt taaaccaaat gaaccgaagc tcacttctgt gccacccgcg
     2941 ataccacggt tacggccttt gtgtacttta cggaattttg tgcgttttgg ttgtaacatt
     3001 cagtgattct ccttatttac gacctttgcc acgtggcgcc tttttaggct gtgccgctgg
     3061 ttctttttca gattcaatca ctgcagccat tccaccaaga atttcacctt tgaagatcca
     3121 cacttttacg ccgattacgc cgtaagtagt gtgagcttca gcagtactat aatcgatgtc
     3181 tgcacgaagc gtatgtagag gtacacgacc ttcacgatac cattctgagc gagcaatttc
     3241 tgcaccacct aaacgaccgc taacttcaac tttgatacct ttagcaccta atttcatcgc
     3301 gttttgaact gcttttttca ttgcacgacg gaacatcaca cgacgttcaa gttgagaagc
     3361 gatgctatct gcgactaatt tcgcatctag ctctggtttt ttcacttcag cgatgttgat
     3421 ttgagctgga acgccagcga ttttcgcaac tgcgttgcgt aatttttcaa catcttcacc
     3481 ttttttaccg ataacgatac ccgggcgagc tgtgtgaata gttacacgaa tgctttttgc
     3541 aggacgctca atagtgatgc gtgatacaga agcgttcgct aactctttgt ttaaaaattt
     3601 acgtactttg aagtcgccgt ctaagttatc agcgaaatct tgtgtattcg cgaaccaagt
     3661 agagttccaa ggtttaacaa tacctaggcg aataccatta ggatgtactt tctgacccat
     3721 ttgttattct cctacttaat taacgatctg acacaaccac agtgatgtgg cttgtacgtt
     3781 ttaaaatacg atctgcacga ccttttgcac gtggcattac acgtttcatg cttggacctt
     3841 catcaacgaa gattttagca actttaagat catcgacatc tgcaccgtca ttatgctctg
     3901 cgtttgcaat agctgactct aatactttct taacaagcgt cgctgctttt ttgttagtga
     3961 aagttaagat ttctaatgct acagaaactt ttttaccgcg aattaaatca gcaactaagc
     4021 gagctttttg ggcagaagta cgagcgtaac gatgtttagc aatagtttcc atcttttacc
     4081 tcttatttct tagctttctt atctgcggcg tgaccgcggt atgtacgagt cggtgcaaat
     4141 tcacctaatt tatgaccgat catttcgtcg gaaacataaa caggaacgtg ctgacgacca
     4201 ttatggactg cgatggtcaa accaatcatt gatggaatga tcattgaacg acgggaccaa
     4261 gttttaattg gttttttatc cccgctttcc accgccttct ctaccttctt caacaagtgt
     4321 aggtcaagga aaggaccttt cttgagggaa cgtggcattg gtgtttacct tttagttaat
     4381 gaattaaatt atttgccacg acgacgtacg atgaatttat cagtacgttt gttgtgacga
     4441 gttttcttac ctttggtttg aacgccccaa ggtgtaacag gatgtttacc gaagttacga
     4501 ccttcaccac caccgtgtgg gtgatctacc gggttcattg cagtaccacg aacagttgga
     4561 cgaatacctc tccaacggtt tgcacctgct ttaccaagaa cgcgaagcat atgctctgag
     4621 ttacctactt caccaatggt tgcagtacac tcagctaata ctttacgcat ttcgcctgaa
     4681 cgaagacgta aagttacata gttaccttca cgggcaatga tttgaacgta agcaccagca
     4741 gaacgtgcaa tttgaccacc tttaccaggt tttaattcaa cgttgtgtac agttgtacct
     4801 actgggatgt tacgcattgg taatgcgtta cctactttaa tcggagctga agcacccgct
     4861 tgaatttgat caccagcagt taaacctttt ggtgctaaaa tatagcgacg ttcaccatct
     4921 ttataaagta ctaatgcgat attcgcagaa cgatttggat cgtattctaa acgctcaaca
     4981 acacccggaa tgtctaattt attacgttta aaatcaatta aacggtagtg ttgtttgtga
     5041 ccgccaccaa tgtgacgagt tgtgatacga cctaagttgt tacgaccacc agttttagat
     5101 ttagtatcta aaagtgctgc aaaaggttta cctttgtgaa gctcagcgtt tacaacttta
     5161 acaacgtgac gacgaccagc ggaggtcggc ttacatttaa cgatagccat ttattttatc
     5221 tcctctgatt actctgccgc accttcaacg aagtcaagcg attgaccttc ttgaagagtt
     5281 acataagctt ttttccagtc actacgacga ccaactttag caccacggcg tttagtttta
     5341 cctttaacta ctacggtacg aacagaatca acttttactt caaaaagttc ttcaactgca
     5401 tttgcaatct caactttgtt tgcatctaat gcaactttga atacgattgt attgcctttt
     5461 tctgcgttgt ttgtcgcttt ttcagagata tgtggtgctt taagcacgtt tagcaaacgt
     5521 tcttgttgaa tcatgctaac atctcctcaa tttgttttac tgcatctacg gttacaacca
     5581 ctttatcgaa agcaattaag ctaactgggt caataccttg cacatcacgc acatcaactt
     5641 tatataagtt gcgtgctgct aagaataaat tctcatctac gtttgcagta atgataagag
     5701 cgtcatttaa cgccatatct tttaattttt gtgctaatac tttagttttt ggtgcatcga
     5761 tttcgaattt ctcaacaacc actaaacggt cttgacgaac taattcagaa agaatgcttt
     5821 tgattgcacc acggtacatt cttttgttca ctttttggct gtgatcttgt ggtttcgcag
     5881 caaaagttac actacccgaa cgccaaattg gtgatttaat atcaccagaa cgtgcacggc
     5941 ctgtaccttt ttgacgccaa ggttttttac ctgaaccaga cacttcagca cgagtttttt
     6001 gagcacgaga tccttgacga gcacctgctg cataagcaac aactacttgg tggattaatg
     6061 cttcattgaa ctcacgtccg aaggtagttt cagaaacagt tagtgcgttt gcacctacaa
     6121 cttgtaattc catctctatc tcctagactt atgctttaac tgccggtttt acgataacat
     6181 caccattagt tgcaccagga acggcacctt taactaataa taatttacgc tcaacatcta
     6241 cacgaacaac ttcaagtgat tgaacggtta cacgctctgc acctaagtgt cctgccattt
     6301 ttttaccttt aaacacacga cctggagttt ggttttgacc aatagaacca agtacacgat
     6361 gtgataaaga gttaccgtga gttgcatctt gagtacggaa gttccaacgt ttaacaccac
     6421 cttggaagcc tttacctttt gatgtaccag taacatcgac ttttttaaca tcagtaaaga
     6481 tgtcaacatt gatttcttga cctaaagtga attcttcacc ttcagtagta cgaaattccc
     6541 ataaaccgcg tccagcttca acacctgctt tcacgaaatg acctgcttct ggtttagtta
     6601 cacggctagc ttttttagag ccagtagtaa cttgaattgc agagtagcca tcgttttcaa
     6661 gggttttaac ttgagtaaca cggttggctt cgatttcgat aacggtaact ggaatagaca
     6721 cgccgtcttc agtgaaaacg cgggtcatac caactttacg accgattaaa ccaatcattg
     6781 taataacctc ttaaaattcc caattaacct agactaatct gaacgtcaac gccagcagct
     6841 agatctaaac gcattaatgc atcaacagtt ttttctgttg gttcaacaat atcaactaaa
     6901 cgtttgtgag tacggatttc atattggtca cgtgcatctt tattcacgtg tggagaaatc
     6961 aatacagtga aacgttcttt acgagttggt aaaggaattg gaccacgaac ttgtgcacca
     7021 gtacgtttag ctgtttctac gatctccgca gtagattgat caattaaacg atgatcaaat
     7081 gcttttaagc ggatacggat tctttggttc tgcattagac cagagctcca attaaaaaat
     7141 ttagctaata aaaaaaccaa actaccactt aacaaattag ttaagggagc tcagttaaac
     7201 ctatatagtt ccaaaattag gaacattgta tacactacaa tatctgtagt gccatttgat
     7261 aaatgattac atcaaatgtc tttagatacc taaagctttg ctatcatact cgtttcattt
     7321 cccttatgca agtaatt
//
LOCUS       DANLXS010000119         7253 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.119, whole genome shotgun sequence.
ACCESSION   DANLXS010000119 DANLXS010000000
VERSION     DANLXS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.119"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            99..695
                     /gene="nfuA"
                     /locus_tag="PXD71_001856"
     CDS             99..695
                     /gene="nfuA"
                     /locus_tag="PXD71_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDL6291946.1"
                     /translation="MEEQTIHISISDAAQAHFARLLEQQEEGTNIRIFVVNPGTPGAE
                     CGVSYCPPNAVEETDTEFKFNRFSAFVDDISLPFLDEAEIDYVTDPMGSQLTLKAPNA
                     KMRKVADDAPFIERLDYVIQTQVNPQLASHGGKVTLIEITEDKYAVLQFGGGCNGCSM
                     VDVTLKEGIEKQLLAMFPDELVGVKDITEHQHGEHSYY"
     gene            complement(863..2500)
                     /gene="pyrG"
                     /locus_tag="PXD71_001857"
     CDS             complement(863..2500)
                     /gene="pyrG"
                     /locus_tag="PXD71_001857"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDL6291947.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPY
                     INVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRK
                     ERRGDYLGATIQVIPHITNEIKARVLEGGKGRDVAIVEVGGTVGDIESLPFLEALRQL
                     AVDIGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPE
                     NEKKKIALFCNVPERAVISLKDVDSIYRIPELLKSQGLDSFVCDRFRLDCKEADLSEW
                     EQVLYCQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTHRLTVNIKYIDSQDIE
                     TKGTELLAGLDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARN
                     VAGLTEANSSEFDKDCSQPVIGLITEWQDAEGNVETRSDESDLGGTMRLGSQQCHLIE
                     GSKARELYGAETIVERHRHRYEVNNKLLPQIEAAGLKVSGLSADRKLVEIIEVPNHPW
                     FIAAQFHPEFTSTPRDGHPLFAGFVKAAKDHQESRKA"
     gene            2764..3813
                     /gene="ansB"
                     /locus_tag="PXD71_001858"
     CDS             2764..3813
                     /gene="ansB"
                     /locus_tag="PXD71_001858"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDL6291948.1"
                     /translation="MKIKKLLLPTLLSIATMPIQAADLPNITILATGGTIAGSQKNTA
                     TSAYQAGQLNIETLIEAVPDIKKLANIKGEQIVKIGSQDMSDEVWLKLAKAINQQCPN
                     TDGFVITHGTDTMEETVYFLDMTVKCEKPVVLVGAMRPATEKSADGVLNLYNSVVVAK
                     DSKSSGRGVLVAMNDIVLGARDVTKTSTTSVQTFNSPNFGALGYIHNSKVDYERSPES
                     KHTVNTPFKVDELTELPKVGIIYAYSNMPTEPLQALLDASYQGIVVAGVGNGNMNAAN
                     LALLEEAAKKGITVVRSSRVPTGYTTRDAEIDDSKYGFIASGTLNPQKARVLLQLALT
                     QTNDIKTIQQYFEDF"
     gene            complement(3887..4531)
                     /locus_tag="PXD71_001859"
     CDS             complement(3887..4531)
                     /locus_tag="PXD71_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291949.1"
                     /translation="MRIWQKLASVIVVTLGLISNASAENFKLKYSSNYLMPAYINFNN
                     GGGQYNIQAKINVPLYNIVFSAKGTEKNEQFHMLSYRDTRNGKTYAMAEIDSKTVKYG
                     KVKEPLKEEALTMPTYDLFTTAFQLSYYDKLPSSFQTTNGKKLYPTPNVQLRKAQKEV
                     KVGGKTYQEITYKFRTSDKKDITVKKFEGEKFPRYIAYSRDGDNYELTFDSFVK"
     gene            complement(4532..5515)
                     /gene="cysB"
                     /locus_tag="PXD71_001860"
     CDS             complement(4532..5515)
                     /gene="cysB"
                     /locus_tag="PXD71_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDL6291950.1"
                     /translation="MKLQQLRYIVEIVNQNLNITEAAEVLYTSQPGISKQVRLLESEL
                     GINIFERNGKHVKGLTPAGEKIVAIARELLVKAQSIKSVAEEQTRPNKGILRIATTNT
                     QARYMLPSVIEKFKARYPEVSLHLHQGSPNQIYDALVAGEVDLAITTEAQYLFDEAVL
                     LPCYWWNRSVIVKPEHPLAAFVNQSKNLTVEQLGRYDLITYTFGFTGQSDLDHAFNKE
                     GILPNIVFTATDADVIKTYVRLGLGVGIMASMAHTETDKDLVAINASHLFQPSMTQIA
                     FKRGTFLRNYMYDFINYFSPHLTRVQVEKAEQLRDNNAISRLFESISLEEK"
     gene            complement(5512..6195)
                     /gene="yjjG"
                     /locus_tag="PXD71_001861"
     CDS             complement(5512..6195)
                     /gene="yjjG"
                     /locus_tag="PXD71_001861"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDL6291951.1"
                     /translation="MKYQWVLFDADETLFSFNSYLGLKAMLARYQIDFSEQDYQDFQA
                     VNKPLWVAYQNNEITAQDIQTRRFVKLSAQTGVDPLQLNQELMAEMAFVSQPLEGVMD
                     MLNALYGKVKMGIITNGFTDLQQKRLDNTQTSHFFDIVVISEQIGAAKPDRQVFDYAF
                     ALMDEFDRTKVLMVGDTLASDILGGNNAGIDTCWLNLLSKENDTDIKPTYEVHTMQQI
                     IAIVEGKSL"
     gene            complement(6271..7164)
                     /locus_tag="PXD71_001862"
     CDS             complement(6271..7164)
                     /locus_tag="PXD71_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HDL6291952.1"
                     /translation="MNKKLPPLNALKAFESAARHLNFTKAAEDLFVTQAAVSHQIKLL
                     EDFLGASLFHRRNRLLELTELGAQYFYEVQPLLEQIALATDKIRQQSSRQILTISVPQ
                     TFGMHWLVPRLADFHEKYPEVEVRIKGVDQDEGLLGKETDVAIYYGKGDWQGLESIRL
                     TESRIVILASPEYLSQNTIHLPADLAGKHLLHSSTHNKWKRMVEHLHIEDIVDAETGS
                     IFSHTFMVLQAAMHQQGIAIANQLLAQHEIEKGTLIEPFPTTLFDEKSFYVVYPSQSA
                     QVQKVQFFVEWIQQKMKECCA"
BASE COUNT         2215 a         1531 c         1316 g         2191 t
ORIGIN      
        1 taacaatttg caaaaatttt tttagctagg caattttgaa attcaggcgt aaaatagttg
       61 ataaattttg taaaatatta aaaaacagga taatttaaat ggaagaacaa accattcata
      121 tttcaatatc agatgcagca caagcccatt ttgccagact attagaacag caagaagagg
      181 gaactaatat ccgtattttt gtggtcaatc ccggcacacc gggggcagaa tgcggtgtct
      241 cttactgccc acccaatgca gttgaagaga cggataccga atttaaattt aaccgtttct
      301 ctgcatttgt cgatgatatt agcttaccgt ttttagacga agcggaaatt gattatgtaa
      361 ccgacccaat gggttcacaa cttaccctca aagcccctaa cgccaaaatg cgtaaagtgg
      421 ctgatgatgc accgtttatc gagcgtttag attatgtgat tcaaacccaa gtcaatccac
      481 aacttgccag ccacggcggt aaagtgacct taattgaaat caccgaagat aaatatgccg
      541 tgctacaatt cggcggtggt tgtaacggtt gctcaatggt ggatgtgacc ttaaaagagg
      601 gaattgagaa acagcttctg gcaatgttcc ctgatgaatt agttggtgtg aaagatatta
      661 ccgaacacca acacggtgaa cattcttact attaaaaata ctaaaaggga taaatcattg
      721 atttatccct ttcttataat taatgagcaa gcggtcaaat ttactcgctt tcgctcgcac
      781 ttcgtgccat cagcaaagcc gatgttcaaa cgcaagcgtt tgtacaaaaa aatgagtaaa
      841 ttcgaccgct tgttaagttt gattaagcct tacgactctc ttggtgatct ttcgcagctt
      901 tcacaaaacc tgcaaataat ggatgaccat cacgcggagt tgaggtaaat tccggatgga
      961 attgtgctgc tataaaccaa gggtgatttg gcacttcaat aatttccact aatttacgat
     1021 ctgccgataa accgctcact tttaagcctg ccgcttcaat ttgtggtaac agcttattgt
     1081 ttacttcgta acggtggcga tgacgttcaa caatcgtttc cgcaccatat aactcacgag
     1141 cttttgagcc ttcaattaaa tggcattgtt gtgagcctaa acgcattgta ccacctaaat
     1201 ctgactcatc gctgcgggtt tccacattcc cttccgcatc ttgccattcg gtaattaagc
     1261 caatcaccgg ctgtgagcaa tctttatcaa attcagaaga atttgcctcg gttaaacctg
     1321 ccacattacg ggcatattca attagggcga tctgcatacc taaacagata cctaagtaag
     1381 gaatcttgtt ttcacgggca tattgtgcgg ttcgaatttt gccttccacg ccacgatagc
     1441 caaagccgcc cggcacaaga atgccgtcta agcctgccaa taactctgtg cctttggttt
     1501 caatatcttg tgaatcaatg tatttaatat tcactgttaa acgatgcgtt aaacctgcgt
     1561 gtttcaaagc ctcattcacc gatttatacg catcaggtaa ttccacatat tttccgacca
     1621 taccgatagt cacttcgccg gttgggtttg cttggcagta tagcacttgt tcccactctg
     1681 aaagatcggc ttctttgcaa tctaaacgga aacgatcgca cacaaaggaa tctaaccctt
     1741 gcgatttcaa taattccgga atgcgataaa tcgaatctac atctttaagc gaaataaccg
     1801 cacgctccgg cacattacag aaaagggcga tttttttctt ctcattttcc ggaatagcac
     1861 gatctgaacg gcaaattaac acatccggct gaataccgat tgaaagcaac tcttttactg
     1921 aatgctgagt cggcttagtt ttcacttccc ctgcggttgg aatataaggc actaaggtta
     1981 aatgcataaa caaggttttt tcacgaccga tatccaccgc taattggcgg agtgcttcta
     2041 agaatggcag tgattcaata tcccccaccg tgccacctac ttctacaatg gcgacatcac
     2101 gccctttacc gccttctaac acacgggctt taatctcgtt ggtaatatgc ggaataacct
     2161 gaatggttgc acctaaatag tcgccacggc gttctttacg caacacttca gagtaaattt
     2221 taccgctggt aaagttgttg gctttgctca tttttgaacg gataaaacgc tcgtagtgac
     2281 ctaaatccaa atcggtttcc gcaccgtctt cggtcacaaa tacctccccg tgctgagttg
     2341 gactcattgt acccggatcg acattgatat aaggatcaag cttcataatg gttacattta
     2401 aaccacgagc ttccagaata gatgctaaag aggctgccgc aatacccttc cctaaagaag
     2461 atacaacacc gcctgtgacg aaaatataat tcgttgtcat tgtgattgcc tttgctgatt
     2521 gattaggttg gaaattcaga atatcagttt acttcgtaaa aaagaaacta tcaggacggg
     2581 aaagtagttt acatcaaatt acattttttt gctaatggaa aatcggcaag cagtcagatt
     2641 aattcttttt tcaataatgc cgactaatct taattaatac actattattt gcgatagatc
     2701 agaattaacc tacatgttta tagatatcat aatacctgtt tttctaatac tggagacgat
     2761 acaatgaaaa tcaaaaaatt actactgcca actctgttaa gcattgctac tatgccaatt
     2821 caagcagccg acctacctaa tattacaatt cttgcaactg gagggacaat tgccggtagc
     2881 caaaaaaata cggcaacctc agcttatcaa gctgggcagc taaatattga aacactcata
     2941 gaagccgtcc cggatattaa aaaattagca aatatcaaag gagaacaaat cgttaaaatc
     3001 ggatctcagg atatgtctga tgaagtatgg ctaaaactgg caaaagcaat taatcaacaa
     3061 tgcccaaaca cagatggctt cgttattacg cacggcacag acactatgga ggaaaccgta
     3121 tacttccttg atatgactgt aaaatgcgaa aaacccgtag tattagtggg ggccatgcga
     3181 ccagcaactg aaaaaagtgc cgacggcgta ttaaatttat ataactcagt tgttgtcgcc
     3241 aaagattcta aatctagtgg cagaggggtg ttggttgcaa tgaatgatat tgtattgggc
     3301 gctcgcgatg ttacaaaaac cagtactaca tcagtgcaaa catttaactc accaaacttt
     3361 ggcgcactag gctatatcca taatagcaaa gttgattatg agcgctctcc tgaaagtaaa
     3421 cacaccgtaa acaccccttt caaagtagat gaattaacgg aattgccaaa agtcggcatt
     3481 atttatgctt attcaaatat gccaaccgaa ccgcttcaag cattattaga tgcgagttat
     3541 caaggcatcg tggttgcagg agttggcaat ggcaatatga atgcggctaa tttggcttta
     3601 cttgaagaag cagctaaaaa aggaattacc gtggttcgct cgtctcgagt gccaaccgga
     3661 tatactaccc gagatgcaga aattgatgat agcaagtatg gttttattgc ttccggcaca
     3721 ttaaatcctc aaaaagctcg agtattactg cagctcgcct taacacaaac aaatgatata
     3781 aaaactattc aacaatattt tgaagatttc taaaaagtaa ataacaagcg gtctattttg
     3841 caaaacaaat cacaaaatag accgcttgtc tcattactaa tctttattat tttacaaagc
     3901 tatcaaaagt tagctcataa ttgtcgccat cacgactata ggcgatatag cgagggaatt
     3961 tttcaccttc aaattttttc accgtaatgt cttttttatc actggttcta aacttgtagg
     4021 taatttcttg gtaggttttg ccgcctactt ttacctcttt ttgggctttg cgtagctgca
     4081 cattcggtgt tgggtaaagt tttttaccgt tagtagtttg aaaactgctc ggtaatttat
     4141 cgtaatagct caactgaaag gctgtggtga acaaatcata agttggcata gtcagcgctt
     4201 cttcttttag tggctctttc actttgccat attttaccgt tttagaatca atttctgcca
     4261 tcgcataagt tttgccgtta cgggtatctc ggtagctcaa catatgaaat tgttcgtttt
     4321 tttctgttcc cttcgcagaa aagacaatat tatataaagg cacattaatt tttgcctgaa
     4381 tattatattg gccaccaccg ttattaaagt taatgtaggc tggcattaaa taatttgagc
     4441 tgtatttcaa cttaaaattc tccgcactgg cgtttgaaat caagcccaaa gtgaccacga
     4501 ttacgctcgc taacttctgc caaattctca tctatttctc ctctaaagat atactttcga
     4561 acaaccttga aattgcattg ttatctcgta attgttccgc tttctccact tgtacccgtg
     4621 ttaaatgtgg cgaaaaataa ttaataaaat catacatata gttacgaaga aaagttcctc
     4681 gtttaaaggc gatttgcgtc atactcggtt gaaacaggtg acttgcattg atagcgacta
     4741 agtctttatc cgtttccgta tgtgccattg atgccataat accaacccct aagcctaagc
     4801 gtacataagt tttgattaca tcagcatctg tcgccgtaaa tacgatatta ggcaaaatac
     4861 cttccttatt gaaggcatga tctaaatccg attgccctgt aaaaccaaaa gtataggtta
     4921 tcaaatcata tctacctaat tgttcgacag tcaaattttt actttggttc acaaaagccg
     4981 caagaggatg ctctggtttg acaataactg aacgattcca ccaataacac ggcaataaaa
     5041 ccgcttcatc aaataaatat tgagcttctg tggtaatggc taaatccact tctccagcca
     5101 ccaaagcatc ataaatttga ttcggggagc cttggtgcaa atgcaaactt acttcaggat
     5161 agcgagcctt aaacttctca attacactcg gtagcatata acgggcttgg gtattggtgg
     5221 tcgcaatgcg taagatgcct ttgttgggtc gagtttgttc ttcagcaacc gatttaatgc
     5281 tctgggcttt taccaataat tcacgagcaa tcgccacaat tttttcacca gcaggcgtta
     5341 gccccttaac gtgcttaccg ttacgctcaa aaatattaat tcccaactca ctttccaaca
     5401 aacgaacttg tttgctaatc cccggttgag aagtgtaaag cacctctgcg gcctcggtaa
     5461 tattcaagtt ctggtttact atttcaacga tataacgtaa ctgttgtagc ttcataatga
     5521 tttcccctct acaatcgcaa taatctgttg catcgtatgc acttcgtaag ttggcttaat
     5581 atccgtatca ttctctttac taagcaaatt taaccaacaa gtatcaattc cagcattatt
     5641 acctcctaaa atatcagaag cgagggtatc gcctaccata agcactttag tacgatcaaa
     5701 ttcgtccatt aatgcaaaag cgtaatcaaa cacttggcga tcaggctttg cggcaccaat
     5761 ttgttctgat atcactacaa tatcaaaaaa atgtgaagtt tgagtattat ctaaacgttt
     5821 ttgctgcaaa tcggtaaaac cattggtgat aatgcccatt tttactttac catataaagc
     5881 atttaacata tccattacac cttctaaagg ctgactgacg aatgccatct ccgccattaa
     5941 ctcttgatta agttgtagcg gatctacgcc tgtttgagca gataatttta caaaacgtct
     6001 ggtttgaata tcttgagcag tgatctcatt attttggtaa gccacccaaa gtggtttatt
     6061 taccgcttga aagtcttgat aatcttgttc actaaaatca atttggtaac gagccagcat
     6121 cgcttttaaa ccaagataag aattaaatga aaacaaggtt tcatcagcat caaataatac
     6181 ccattgatat ttcataatat tcctttttta acttagataa caagcggtca aatttttcta
     6241 atttttcgca taccgctgat aaagccacgc ttaagcacaa cactctttca ttttttgctg
     6301 aatccactca acaaaaaatt gtactttctg aacttgggct gattgcgaag gatagacaac
     6361 atagaacgat ttttcatcaa acaatgtggt tgggaaaggc tctattaaag tacctttttc
     6421 aatttcgtgc tgtgccaaca actggttggc aatcgcaatg ccttgttggt gcattgcagc
     6481 ttgaagcacc ataaaagtat ggctaaaaat tgagccagtt tccgcatcta ctatatcttc
     6541 tatatgcaaa tgttcaacca ttcttttcca cttattatgg gtggaggaat gtaataaatg
     6601 cttacctgct aaatccgccg gtaaatgaat ggtattttgc gataaatact caggcgaagc
     6661 taaaataaca atacgggatt ccgtcaaacg gatagattcc agaccttgcc aatccccctt
     6721 accataataa atagcaacat ctgtttcttt acctaataat ccctcatctt gatcaacacc
     6781 cttaatacgt acttccactt ctggatattt ttcgtggaaa tccgccaaac gtgggactaa
     6841 ccaatgcata ccaaatgttt gtggtacgct aatagtcaga atttgacgac tactttgctg
     6901 cctaatttta tctgttgcga gagcaatttg ttctaataat ggctgaactt catagaaata
     6961 ttgtgcgcct aactcagtta attccagcaa acggttacgg cgatggaaca gggaagcacc
     7021 taaaaaatct tctaatagtt taatttggtg gctcactgca gcttgtgtga cgaagagatc
     7081 ttctgccgct ttagtaaaat ttaaatggcg tgcagcagat tcaaatgctt tcaatgcatt
     7141 gagtggtgga agttttttgt tcataatttt tcaatctaat agattactaa acaagcaact
     7201 ctattatttt ttctgatagt ttaaataaaa aaaattcgtt tgatgcttgc aaa
//
LOCUS       DANLXS010000120         7212 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.120, whole genome shotgun sequence.
ACCESSION   DANLXS010000120 DANLXS010000000
VERSION     DANLXS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.120"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(90..653)
                     /locus_tag="PXD71_001863"
     CDS             complement(90..653)
                     /locus_tag="PXD71_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5358 domain-containing protein"
                     /protein_id="HDL6291953.1"
                     /translation="MLRKIVLLSTGLLLAACSSNEPQQPEAFPGEFANADYVLSEENA
                     QKWVIASEQAKQCIYPNLTRIQQEHFSKEDSYIFSQYVFFYPLEEIIGEQYVKIIQND
                     EKSMGYAQYLFKKLKNNEAVEALPKKQCATLRTNAKNDLAVVKGQYQSGMIEETNSQE
                     GKSGDKVATNDNKFFFDIIKWGSMLLL"
     gene            803..3400
                     /gene="mutS"
                     /locus_tag="PXD71_001864"
     CDS             803..3400
                     /gene="mutS"
                     /locus_tag="PXD71_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL6291954.1"
                     /translation="MTQDFSTHTPMMQQYLQLKAQNPDILLFYRMGDFYELFYDDAKR
                     AAALLDISLTKRGQSAGEPIPMAGVPYHAVEGYLAKLVALGESVAICEQIGDPATSKG
                     PVERKVVRIVTPGTVSDEALLPERQDNLVAAVYEEKGTFAIATLDMTSGRFLISELAS
                     KEALSAELQRVQPAEILYAEDFSAASILTNYKGLRRRPVWEFELVTAIQLLNRQFGTQ
                     TLAGFGVEKAVVALCAAGCVLHYAQETQRTALPHINAIHLAQHSDTILLDAATRRNLE
                     LTQNLAGGTENTLAAVLDKCVTPMGSRLLKRWIHQPIRDCGKLQARQKAITALIQYER
                     MSDLQPLLQQVGDMERILARVALRTARPRDLTRLRTALAQLPEIAKNSQNLTASIDQL
                     LAQLADFSPLHDLLERAIIENPPQLIRDGGVIAEGYNAELDEWRQLSDGATQYLENLE
                     IRERESTGIDSLKIGFNAVHGYYIQISQGQAHKAPIHYVRRQTLKNAERYIIPELKTY
                     EDKVLKAKGASLALEKQLYDELFDVLMPRLGELQLAAMMLSELDVLTNLAERADSLGY
                     VCPSFSPMRMVNIKGGRHPVVEQVLTEPFIANPVFLNSQRHLLIVTGPNMGGKSTYMR
                     QIALITLMAYIGSFVPAESAEIGPIDRIFTRIGASDDLASGRSTFMVEMTEMANILHQ
                     ATENSLVLIDEIGRGTSTYDGLSLAWACAEWLAKKTQSLTLFATHYFELTGLPNQLKG
                     VANVHLDAREHQDSIVFMHSVQEGAASKSYGLAVAALAGVPKQVIQLAKQRLAHLEEI
                     SHQTKQANEHPQADLLFSDGFDLQKNEAIQPLVVENEVEKALKALQPDELTPRQALDE
                     LYRLKKLLG"
     gene            complement(3448..4902)
                     /locus_tag="PXD71_001865"
     CDS             complement(3448..4902)
                     /locus_tag="PXD71_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6291955.1"
                     /translation="MKKIVSTLLILTSNLAFAETTPAQLQEKMDNQRMQKNLHTLEKP
                     EPQAKPQAVNAQAHRQSISISSQQLLQQPELLNSAMLTALVTNHTENVQFLLPIYQQL
                     PASKQNSEVVQWAEAVNAKAKQNYSEAIKGYRQLLAKYPENQPIRMQLAVALFENREN
                     EAAEAQFYKLQADKLPQEIANAVNSYLEAIGKQDQWSFQGGLTYLNDPNINNAPNAGT
                     TYGNWTAPKKESAQGVGFHFEADKKWSWGNGFFHEFRLNGNGKYYWNNKKYNEYSLRE
                     SLGLGYQNAKQKITLLPFFEQMWYAGGSSQTESTKRYSNAGGVNARWQYWITPQWQSA
                     ISYEYAEQRYTRRQHLDGNYHFIAPSLYFYPNSRQYWFVGANFNRTSPRDLDDSFIRR
                     GVSIGWGQEWKNGLSTQLSASYARKQYRAPMPIFQITQRNSEYGVQASIWHRALHFKG
                     ITPRLTWQYNKVKSNHSFYSYDKNRVYIEFSHTF"
     gene            complement(5126..5764)
                     /locus_tag="PXD71_001866"
     CDS             complement(5126..5764)
                     /locus_tag="PXD71_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL6291956.1"
                     /translation="MRYTTEQIIEKLRQLKVVPVIALDKAEDILPLAKTLSENGLPVV
                     EITFRSEAAEEAIKLVQANYPEVLIAAGTVLTKEQVLTAKNAGADCIVTPGFNPTIVK
                     YCQVLGLPVTPGVNNPMAIEAALELGIQAVKFFPAEASGGVKMIKALLGPYGNLQIMP
                     TGGISPQNINDYLAIPNVVACGGSWFVEKSLIKEQKWEEIGKLTKEVVELVK"
     gene            complement(5852..6799)
                     /locus_tag="PXD71_001867"
     CDS             complement(5852..6799)
                     /locus_tag="PXD71_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL6291957.1"
                     /translation="MKKLAILGECMIELNGEPFGEMHQTYGGDSLNTATYLARVSSPE
                     QIEVRYISALGTDKLSQGMLAAWQVDNINTDFVLRDEHRQPGLYLIQLDKQGERTFLY
                     WRNQSAARYLLQHPGYPKALSALNEVDMVYLSGISLAILPENDRTLLIAQLRELKTQG
                     VKIAFDSNYRPKLWESVAAAQAHYAELLQLVDLALVTFDDEQLLWGDVNEQATFDRLT
                     QYGIQTIVVKQGSLGATVLQNGERTFVPTVAVEKVVDTTSAGDSFNAGFLNGYLLGKD
                     VVTCCQQGNAVAGIVIQHKGAIIAKSATTHLKSQFEQSI"
     gene            complement(6816..>7212)
                     /locus_tag="PXD71_001868"
     CDS             complement(6816..>7212)
                     /locus_tag="PXD71_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6291958.1"
                     /translation="MLGKEGANIINISSMNAFTPLTKIPAYSGAKSAISNFTQWLAVY
                     FSKVGIRCNAIAPGFLVSNQNKALLFNADGTPTARANKILTNTPMGRFGQADELVGAI
                     LFLMDHHYSSFINGVVLPVDGGFSAYSGV"
BASE COUNT         1896 a         1580 c         1689 g         2047 t
ORIGIN      
        1 gtattttaag actgaggctt tagataaaag ttcaatcggt aggtataaat ggctcggttg
       61 tggttaaaac aaccgagtat tgaggctcac tagagaagta acatactgcc ccatttgata
      121 atatcgaaga agaatttatt atcattggtg gcaactttat cgcctgattt cccttcttgg
      181 ctattggttt cttcgatcat accgctttga tactgccctt ttaccactgc caaatcattt
      241 tttgcattgg tgcgtaaggt cgcacattgt tttttcggta acgcttcaac cgcttcgtta
      301 tttttcagtt ttttgaataa atattgagcg taacccattg atttttcatc attttgaatg
      361 atttttacat actgctcccc gataatctct tcaagcggat agaaaaatac atattgtgag
      421 aaaatataag aatcttcttt ggaaaaatgc tcttgctgaa tacgggttaa attggggtag
      481 atacactgtt ttgcctgttc gctggcaatt acccattttt gagcattttc ttccgaaagc
      541 acataatcgg cattggcaaa ttcgcccggg aatgcttccg gttgttgcgg ttcattgctg
      601 ctacaagccg ccaataataa gcctgtagaa agtagcacta tttttcttaa cattctgacc
      661 tctgttttaa aaatttggtg cattgtaaca aaaaaacagc ggctgaccat tgcaaatttt
      721 cagcgaaaaa tgaccgcttg tttttatccc ttggctgaat ctgttaaact atcagcaatg
      781 atttctcaca ataaatagac tgatgacaca agatttctcc acccatacgc caatgatgca
      841 acaatatttg caactcaaag ctcaaaatcc ggacatacta ttgttttacc gaatggggga
      901 tttttatgag ctgttttatg atgatgcgaa acgagcggca gcattgttgg atatttcgct
      961 caccaagcga gggcaatctg ccggtgagcc gattccgatg gcgggtgtgc cttatcacgc
     1021 agtggagggg tatttggcga aattggtggc gttgggggaa tcggtagcaa tttgtgaaca
     1081 gattggcgac ccagcaacct caaaagggcc ggtagaacgc aaagtggtac ggattgttac
     1141 gccgggaaca gttagcgatg aggcattgtt acccgaacgc caagataact tggtggcggc
     1201 agtttacgaa gaaaaaggca catttgcgat tgctacgcta gatatgacct ccgggcggtt
     1261 tttaatcagt gaacttgcta gcaaagaggc tctatccgcc gagttacaac gtgtacaacc
     1321 ggcagaaatt ctgtatgcgg aagatttttc cgctgcctca attttaacga attacaaagg
     1381 gttacgccgc cgtccggtgt gggaatttga gctggtaacg gcgattcagc tgctaaatcg
     1441 ccaattcggc acgcaaacac ttgccggatt tggggtggaa aaagcggtgg tggcactttg
     1501 tgctgccggc tgtgtcttgc attatgccca agaaacgcaa cgcaccgctt taccgcacat
     1561 taacgcaatt catttggcac aacatagcga tactattttg ctcgatgcag ctactcgtcg
     1621 caatttagag ctgacccaaa acttagcggg tggcacggaa aacacgctgg cggcggtgtt
     1681 agataaatgt gtgaccccaa tggggagccg tctgctcaag cgttggattc atcagccgat
     1741 tcgagattgt ggtaaattac aagcccgaca aaaggcgata acagcattga tacaatatga
     1801 gcgaatgtcg gatttacagc cgttattaca acaagtcggc gatatggagc ggattctcgc
     1861 ccgagtggcg ttgcgtacgg ctcgccctcg tgacctcacc cgactacgca ctgcattggc
     1921 tcagttaccg gaaattgcaa aaaattcaca aaatctgacc gctagtatcg atcagctttt
     1981 ggctcaactg gcggatttca gcccgttaca cgatttgctg gagcgggcga ttatcgaaaa
     2041 cccaccacaa ctcattcgtg atggtggggt gattgcagaa ggttataatg ccgagcttga
     2101 cgaatggcgg caactttcgg acggggcaac gcaatatctg gaaaatttgg agatcagaga
     2161 acgtgaaagc accggcattg attcgctgaa aatcggcttt aatgcggttc acggctatta
     2221 cattcagatc agccaagggc aagcacacaa agccccgatt cactatgttc gccgccaaac
     2281 gctcaaaaat gcggagcggt atattatccc tgagctgaaa acttacgaag acaaagtgct
     2341 gaaagcaaaa ggggcatcgc ttgcccttga aaaacagctt tatgatgaac tgtttgatgt
     2401 gttaatgcct cgcttaggcg aattgcaatt agcggcgatg atgctgtctg aacttgatgt
     2461 cttaaccaac cttgccgagc gagctgatag tttaggctat gtttgcccga gctttagccc
     2521 gatgcgaatg gtgaatatta aaggcggcag acacccggtg gtggagcagg ttttaaccga
     2581 gccgtttatt gccaatccgg tgtttttaaa ttcccaacgg catttattga ttgtgaccgg
     2641 cccgaatatg ggcggtaaaa gcacctatat gcggcaaatt gccctcatta cattgatggc
     2701 gtatatcggc agttttgtgc cggcagaaag tgcggaaatc ggcccgattg accgtatttt
     2761 cacccgaatt ggggcaagcg atgatttagc ttcagggcgt tcaaccttta tggtggaaat
     2821 gaccgaaatg gcgaatattc tgcatcaagc caccgaaaac agccttgtgc tgattgatga
     2881 aatcgggcga ggcacttcta cctatgacgg cttatcactg gcgtgggcgt gtgccgaatg
     2941 gctggcgaag aaaacccagt cactcacttt gtttgcaacc cactattttg aacttaccgg
     3001 cttgccgaat cagctcaaag gggtggcaaa tgtgcattta gacgcgcgtg aacatcaaga
     3061 cagtattgtg tttatgcact cggtgcaaga gggggccgcc agcaaaagtt acggcttggc
     3121 ggtggcagcc ttggcgggtg tgccaaagca agtgattcaa ctggcaaagc agcgtttggc
     3181 acatttggag gaaatttccc accaaaccaa acaagcaaac gaacatccac aggcggattt
     3241 gttgttttcc gatggttttg atttgcaaaa aaatgaagca attcaaccgc ttgtagtaga
     3301 aaatgaagtt gaaaaagccc tgaaagccct tcagcctgat gaacttaccc caagacaggc
     3361 tttggacgag ttgtatcgct tgaaaaaatt gttggggtaa attgaaaaaa taactaagaa
     3421 atcgccccgt ttaaggggcg tttttgttta gaacgtatgt gaaaactcaa tatagacacg
     3481 gtttttgtca taactataga agctatgatt gctttttacc ttgttatatt gccacgttaa
     3541 gcgaggggta atacccttaa aatgcaaggc tctgtgccag atgctggctt gcacaccata
     3601 ttcactgtta cgttgtgtaa tttggaaaat cggcattggg gctcggtatt gtttgcgggc
     3661 gtagctggca cttaattggg ttgaaagccc gtttttccac tcttgtcccc aaccaataga
     3721 aacacctcgg cgaataaagc tatcatctaa atcacggggg ctggtgcggt taaaattcgc
     3781 ccccacaaac caatattgcc ggctgttagg gtaaaaatag aggctcgggg caataaaatg
     3841 ataattgccg tctaaatgtt ggcggcgagt gtaacgctgt tccgcatatt cgtagctgat
     3901 tgcagattgc cactgtggag taatccaata ttgccagcgg gcattcacac cgcctgcgtt
     3961 ggagtagcgt ttggtgcttt cggtttggct tgagccaccg gcataccaca tctgctcaaa
     4021 aaacggcaac aaggtgattt tttgttttgc attttgataa cccaagccga ggctttctct
     4081 gagcgaatat tcgttatatt ttttgttatt ccagtaatat ttgccgtttc cgtttaaacg
     4141 aaactcgtgg aagaaaccat tgccccaaga ccacttttta tccgcctcaa agtgaaagcc
     4201 gaccccttgg gcggattctt ttttcggtgc agtccagttg ccataggtcg tgcctgcatt
     4261 tggagcattg ttgatattcg ggtcattcaa ataggttaag ccgccttgaa aactccattg
     4321 atcttgcttg ccaatcgcct ccaaatagct attgaccgca tttgcaattt cttgcggtaa
     4381 tttgtccgct tgtaacttat aaaattgagc ctctgcggct tcattttccc gattttcaaa
     4441 caatgcaacc gctaattgca tacggattgg ctgattttca gggtattttg ccagtagctg
     4501 acggtagcct tttattgcct ctgaataatt ttgttttgct ttggcattaa ctgcctccgc
     4561 ccattgtaca acttcgctgt tttgtttact ggcgggtaat tgttgataaa taggcagtaa
     4621 gaattgaaca ttctcagtgt ggttagtgac caacgcagtt agcattgccg agttgagcaa
     4681 ctcaggctgt tgaagcagct gttggcttga aatagagatc gattgccggt gagcctgtgc
     4741 attaaccgct tgtggtttgg cttgaggttc aggcttttct aatgtatgta ggtttttctg
     4801 cattcgctgg ttatccattt tttcttgaag ttgagccggt gtggtttccg caaatgccag
     4861 gtttgaggta aggattaaaa gcgttgagac tatttttttc ataaagctat cttttatttg
     4921 aatagaaaag gctatttttc aatagccttt agtgaagagt tttgaggttg ttttgctata
     4981 tttttcatta agttaaatat atagctttaa atatacctac tatatgctac taaataaagt
     5041 atgcaatatt tgttatgctt tgtaaataag aggaaagaga tccattttgg gtagaaaata
     5101 gctcattgct tggaacttta aatggttatt tcaccaattc aaccacttcc ttcgtcaatt
     5161 tcccaatctc ctcccacttc tgttccttaa tcagggattt ctccacaaac caagagccgc
     5221 cgcaggcgac tacgttggga atggcgaggt agtcgttgat gttttgtggc gagatgccgc
     5281 cggtcggcat aatctgtaag ttgccgtaag ggccgagcag ggctttaatc attttcacgc
     5341 cgccactggc ttcggcaggg aaaaatttaa ctgcttgtat gccaagttcg agggcggctt
     5401 caatcgccat cgggttgttc acacccggtg taacaggcaa acctaacact tggcaatact
     5461 taacgatagt cggattaaaa cccggagtca caatgcaatc cgccccagcg tttttggcag
     5521 ttaagacttg ctctttggtc agcactgtgc ctgctgcaat caatacttcc ggatagttgg
     5581 cttgcactaa tttaattgcc tcttcagctg cttccgagcg gaaagtgatt tccaccaccg
     5641 gcaaaccgtt ttcggaaagg gttttcgcca gcggtaaaat atcttctgct ttatccaaag
     5701 caatcacggg aacaactttc agttgtctta atttttcaat gatttgttcg gtggtgtaac
     5761 gcatttctag cctcattatg ttgtattgca agcggtcgct tttgtaaaaa attttgcaaa
     5821 tttaaccgct tgtcgttgtg ctattgttgg tctaaatgga ttgttcaaac tgcgatttca
     5881 aatgagtagt ggcggatttg gcaataatcg cccctttgtg ctgaatgacg ataccggcaa
     5941 cggcatttcc ttgttggcaa caggtaacga catctttacc caataagtaa ccgttaagga
     6001 agccggcatt aaaggaatcg ccggcggaag tggtatcgac caccttctct accgcaacag
     6061 ttggcacaaa agtacgctcc ccgttttgca acaccgttgc ccccagagag ccttgtttca
     6121 ccacaatggt ctgaatgcca tattgagtga gacggtcaaa ggtggcttgc tcattcacat
     6181 cgccccaaag cagttgctcg tcatcaaagg tcacgagagc taaatcgact aattgcaaca
     6241 gctcggcata gtgagcttgg gcagcagcca cactttccca cagtttcggc cggtaattgc
     6301 tgtcaaaggc gattttcacc ccttgtgttt tgagctcacg caactgtgca atcagcaaag
     6361 tgcggtcgtt ttccggcaag atggcaaggc tgataccgct caagtaaacc atatctacct
     6421 cgtttagtgc cgagagtgct ttgggatagc cggggtgttg caacaaataa cgggcggcag
     6481 attgatttcg ccaatagagg aacgttcgct cgccctgttt atccaactga atcagataca
     6541 gccccggctg gcggtgctcg tcacgcaaca caaaatccgt gttgatatta tccacctgcc
     6601 acgccgctaa cattccctga ctgagcttat ccgtgccgag agcggaaata taacgcacct
     6661 caatctgttc aggcgaactc acccgagcca aataggtagc ggtgttgagc gaatccccac
     6721 cataggtttg gtgcatctcc ccaaacggct ccccgtttaa ctcaatcata cattcaccga
     6781 gaatggcgag ttttttcata ggaatacctt ctaagttaca cgccgctata agcagagaat
     6841 ccgccatcaa caggtaatac aacgccattg ataaagctgg aataatggtg atccattaaa
     6901 aataggattg cacctaccaa ttcatcggct tgtccgaaac gtcccatagg ggtattcgtg
     6961 aggattttat tagctctagc tgttggcgta ccatcggcgt taaatagtag agctttattt
     7021 tgattactca ctaaaaatcc cggtgcaata gcattacagc ggatacctac ttttgagaaa
     7081 taaactgcta accattgtgt aaagttactg attgcagatt ttgctccaga ataggcaggg
     7141 attttagtta atggagtaaa ggcattcata ctggatatat taatgatatt agcgccctct
     7201 ttacccaaca ta
//
LOCUS       DANLXS010000121         7200 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.121, whole genome shotgun sequence.
ACCESSION   DANLXS010000121 DANLXS010000000
VERSION     DANLXS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.121"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            89..1180
                     /gene="ulaG"
                     /locus_tag="PXD71_001869"
     CDS             89..1180
                     /gene="ulaG"
                     /locus_tag="PXD71_001869"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL6291959.1"
                     /translation="MSKIQEITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     CVGVWIKTPAGANICMDLWCGRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVL
                     DPFAINEVDFILASHYHSDHIDVNVAAAICNNPKLDHVKFVGPWHCAELWKKWGVPEE
                     RIIVVKPGDVVKLKDVEIHALDSFDRTCLVTLPVEGAEERGGELAGLCPSDEEMGRKA
                     VNYVFKTPGGTIYHGADSHYSIQFAKHGKQFDIDVALNNYGENPVGIADKMTSVDLLR
                     MAECLRCKVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYQFHPFIWEVGGKYVYPR
                     DKDLIEYHHPRGFDDCFEQEPNIQFKSML"
     gene            1264..2010
                     /gene="ulaR"
                     /locus_tag="PXD71_001870"
     CDS             1264..2010
                     /gene="ulaR"
                     /locus_tag="PXD71_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDL6291960.1"
                     /translation="MNESYRHKELLALLEERKALSTADIMQLLNISPATARRDISKLD
                     GQGKLKKVRNGAEAILAPTFLQHTKITNNAQEKRRIAEAAGRLCKDGESLILTCGSTM
                     EMLGEVLCGRKLQIITNYLPLANQLIAGCHDDVVIMGGQYNKHKKVMLSINTQNEMTY
                     AATTMFTSGKGLTSEGLFKTDMIIANAEQQMAKHAERYVVLLDSTKLGKKVGMLFKEF
                     DKIDLLITGKEADPTIIQALRNKGLDIILA"
     gene            2065..2925
                     /locus_tag="PXD71_001871"
     CDS             2065..2925
                     /locus_tag="PXD71_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL6291961.1"
                     /translation="MRKHKLGIYEKALPKNISWQDRLSIAKVCGFDFVEMSIDETDER
                     LARLDWSKKERAKLVKTIINTGVTIPSMCLSGHRRFPFGSCDEATRQKAYEIMEKAIQ
                     LAVDLGIRTIQLAGYDVYYEEQDEGTIQRFQQGLEWAVELAASNQVTIAVEIMDTQFM
                     SSISRWKKWDEIIKSPWFTVYPDLGNLSAWNDNVAEELKLGIDKISAIHLKDTYKVTE
                     TCKGQFRDVPFGDGCVDFAACFKTLAELNYRGAFLIEMWTEKAEEPITEIINARRWIE
                     QKMQEGGFTL"
     gene            2922..3641
                     /locus_tag="PXD71_001872"
     CDS             2922..3641
                     /locus_tag="PXD71_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL6291962.1"
                     /translation="MTQLTELTPALQALRERVLKANLELPKYGLVTFTWGNVSEIDRE
                     TGLVAIKPSGVEYDAMTVEDIVLVDLQGNRVYGDKKPSSDTETHLELYRQFTDIGGVV
                     HTHSRNAVGWAQAGRDLLALGTTQGDYFYGAIPCTRKMTAEEIAGSYELETGKVIVET
                     FRTRGIEPKAVPGVLVHSHGPFTWGKDGHDAVHNSVVLEEVAYMNFISHQIRPQIGPM
                     QQELLDKHYLRKHGANAYYGQ"
     gene            3862..6339
                     /locus_tag="PXD71_001873"
     CDS             3862..6339
                     /locus_tag="PXD71_001873"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012311.1,HMM:TIGR02601.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL6291963.1"
                     /translation="MYLLEGNQKVKNRNYFVAIATILALSPEVNATELSDQLASRTRY
                     YPSATDSYYSYYKYAPIYSGYYTSNSRNGYSSWYYPGSNAGVTTRTVSTTVNGITVTK
                     VYTTHNGRTTVRTTYAPAVNKPIVNKPTPIANKPVVDTPTQPVIDANPIMDPPVQPIV
                     DNKPITDTQPNPITDNKPVIESPIDDTRPVIIRPTTPVNLHPEPVAVGVPNVGIRWND
                     RSRGYNIDDPNNKQNVKLTGEGVKVGILDSGFKNYLMQDDILKKFGNRAVIIETGRTA
                     PASATHGIEVAEMVGGGNSRNGVASKSTLLLADITKTTNAGTGLSATAPIYYDLWNRG
                     ARIFNQSYGIPKPLTYFNNNRNSSYYYLHQFDGNVLQFYKDKVKEGGLFVWAAGNNRG
                     DLDPSLQAGLPHYEADLEKGWISVVAVATRTNQGLGNFEWSNLLPYSQAGVAKNWTVT
                     AMGDYIFNLGGRNIIASGSSFAAPAVTGTAALVKQKYPWMDGNLIKQSILSTATDIGA
                     PGVDDVYGWGLLNIEKATNGPARFGKRLALADNVTVNIPNGSYEFSNNINGDAGLVKN
                     GQGELILSGASTFEGDTTLNEGAVTINGKKYVSKVNVSPSGTLVVNNTTLENGVNNEG
                     TVVNQGYSTIKRAYVASAQSTLVSDLGSNLNVKGEVDLNGSTLSLAAVKEGKAQYVTQ
                     KGLSMSAITSDSAIKGNFAAIETSGLLTATAEKTNENEVKATVSRKNVATALESSFSA
                     LDNQIEGNNGSTNATNDEFAQQAATLQNSIANINTQSVVLDSLSGQIYASAQALTFEN
                     SETVNKDLSNRLVMLGTLDNVGETAGV"
     gene            6349..7164
                     /locus_tag="PXD71_001874"
     CDS             6349..7164
                     /locus_tag="PXD71_001874"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6291964.1"
                     /translation="MYGNGTLKEIGFGEGKTNTYAGQIGFDKPVTDHVILGAALNYSK
                     AKVNFDRYGGSSKADGIGASLYARIGNKHKTPWYLQGRAGYGSVDSDVERHIILADNN
                     ASTAKINHRDRVFSAYVESGYDFKQGRFALTPFVGFSHDVVRRGAFSEQNSQFGLTAD
                     KATYKQTSGLIGLRTSLEVNLAGMKTTFQGYVNHQKAFNREDLSFKASYTGLQDAKFD
                     VKGIGLAKHKTWVGIGALAEVNKNTSLYVNYDLKLAKNSGHNNVVTAGIRINF"
BASE COUNT         2250 a         1342 c         1627 g         1981 t
ORIGIN      
        1 gaataaaaaa gatgtacaaa tttttgtgat tgtttatgat tggttttgat tgcattgttc
       61 ttattttagc gtaaatcagg agactattat gagcaaaatt caagaaatca ctcgtgaaag
      121 ctggatcctt tcaactttcc ctgaatgggg tacttggttg aatgaagaaa ttgaagaaga
      181 agttgtaccg gaaggcaatt ttgcaatgtg gtggctaggc tgtgtcggtg tgtggattaa
      241 aaccccagct ggtgcgaata tctgtatgga tttatggtgc ggtcgtggta aatccaccaa
      301 gaaagtaaaa gatatggtgc gtggtcatca aatggcaaat atggcagggg tgcgtaagct
      361 ccagcctaat ttgcgggctc aaccgatggt gttagatccg tttgcgatta acgaagtgga
      421 cttcattctc gcttctcact atcactcaga ccatattgat gtaaacgtgg cagcggctat
      481 ttgcaataac ccgaaattag accacgttaa atttgtcggc ccttggcact gtgcagaatt
      541 atggaaaaaa tggggcgtgc cggaagaacg tattattgtg gtaaaaccgg gcgatgtcgt
      601 gaaattaaaa gatgtagaaa ttcacgccct agattcattt gaccgtacct gtttagtgac
      661 cttaccggta gaaggtgcag aagaacgtgg cggagaactt gcaggcttat gcccatcaga
      721 tgaagaaatg ggacgcaaag cggtgaacta tgtgttcaaa accccgggcg gtacgattta
      781 tcacggtgca gattctcact actcaatcca attcgctaaa cacggtaagc aatttgatat
      841 tgacgtagcg ctgaataact atggcgaaaa cccggttggt attgcggata aaatgacttc
      901 agttgattta ttacgtatgg cggagtgttt acgctgtaaa gtgattattc cggttcacca
      961 tgatatttgg acaaacttta tggcaagtac gcaagaaatt attgatttat ggcgtatgcg
     1021 taaagatcgc ttacaatacc aattccaccc atttatttgg gaagtgggcg gcaaatatgt
     1081 ttacccacgt gataaagact taattgaata tcatcaccct cgcggctttg atgactgctt
     1141 cgagcaagag ccaaatattc aatttaaatc catgctatag gtgagcagtc gccactctca
     1201 ctggagagtg gcgttttagc aagttcatta tggagcagta gtgattagta atgggataaa
     1261 gtagtgaacg aaagttatag acacaaagag ttattagccc tgttggaaga gcggaaagca
     1321 ctgtcaacag cagatattat gcagctgtta aatatttctc cggcaacagc aagacgtgat
     1381 atcagtaaat tagatggtca aggtaaatta aaaaaggtta gaaacggagc agaagcaatt
     1441 ctggctccta catttttaca gcacacaaaa ataactaaca atgcgcagga gaaacgtaga
     1501 atagcggaag ctgccggtcg tttatgtaaa gatggggaaa gtcttattct gacgtgtggt
     1561 tccacaatgg aaatgctcgg tgaagtatta tgtggtagaa aattgcaaat tattaccaat
     1621 tatctaccgc ttgcaaacca gttgattgca ggttgccacg atgatgtagt gattatgggt
     1681 gggcaatata ataaacataa aaaagtgatg ctctcaatta acacccaaaa tgagatgact
     1741 tatgccgcta ccacaatgtt taccagtggc aaaggcttaa ccagcgaagg attatttaaa
     1801 accgatatga ttattgccaa tgccgagcaa caaatggcaa agcacgcaga acgttatgtg
     1861 gtgttattgg atagcaccaa gctcggcaaa aaagtcggta tgctgtttaa agagttcgat
     1921 aaaattgatt tattgattac cggcaaagag gcggatccga ctattatcca agcattaaga
     1981 aacaaaggcc tggatattat cttggcttag cctgtagcgg tcaaatttaa tcaaaatttt
     2041 acttatcatc ttcaagggaa aactatgaga aaacataaac tgggtattta cgaaaaggcc
     2101 ttgccgaaaa acatttcgtg gcaagaccgc ctttctatcg ccaaagtctg cggttttgac
     2161 tttgtggaaa tgtcgattga cgaaacggac gaacgcttag cccgccttga ttggagcaaa
     2221 aaagaacgtg ccaagctggt gaaaaccatc atcaacaccg gcgtgaccat tccgtcaatg
     2281 tgcttgtccg gtcatcgccg tttcccgttt ggttcttgtg atgaagcgac ccgccaaaaa
     2341 gcctatgaaa ttatggaaaa agccattcaa ctggcggtcg atctcggtat tcgcaccatt
     2401 cagcttgccg gctatgacgt ctattacgaa gagcaggacg aaggcacaat ccaacgcttc
     2461 caacaaggct tagaatgggc ggtggagctg gcggcaagca accaagttac cattgcggtg
     2521 gaaattatgg acacccaatt tatgagttcg atttcccgct ggaaaaaatg ggacgagatc
     2581 atcaaatcac cgtggttcac cgtgtatcca gatttaggca acctctcagc gtggaacgac
     2641 aacgtagcgg aagagctgaa actcggcatc gacaaaatct ctgccattca cttaaaagac
     2701 acttacaaag tgactgaaac ctgcaaaggg cagttccgtg atgtgccatt tggtgacggt
     2761 tgcgtagatt tcgccgcttg ttttaaaacc cttgccgagc tgaattaccg tggcgcattc
     2821 ttaattgaaa tgtggacgga aaaagccgaa gagccgatta ctgaaatcat caacgcccgc
     2881 cgttggatcg aacaaaaaat gcaagaagga ggatttaccc tatgacgcaa ttaactgaat
     2941 taacccctgc attacaagcc ttaagagagc gtgtgttaaa agccaattta gaattaccga
     3001 agtatggctt ggtaactttc acttggggta acgtaagtga aatcgaccgt gagaccggtt
     3061 tagtcgccat caaaccttcc ggcgtagaat acgatgcgat gacggtggaa gacattgtat
     3121 tggttgattt acaaggtaat cgagtttacg gagataaaaa accgtcttct gatacagaaa
     3181 cgcacttaga actttatcgc caattcactg atattggcgg tgtggtacac actcactctc
     3241 gcaatgctgt aggttgggca caagcagggc gtgatttatt ggcacttggc accactcaag
     3301 gcgattattt ctacggtgcg attccttgta ctcgcaaaat gaccgcagag gaaattgccg
     3361 gcagttatga gctggaaacc ggaaaggtga ttgtcgaaac tttccgaact cgaggtattg
     3421 aaccgaaagc agttccaggt gtgttggtac attcacacgg tccatttacc tggggaaaag
     3481 atggacacga tgctgtgcat aattctgtgg tattagaaga ggtggcttac atgaatttta
     3541 ttagccatca aattcgccca caaattggcc caatgcaaca ggaattatta gataagcatt
     3601 atctgcgtaa gcatggtgca aatgcttatt atgggcaata gctaataacc taaaaattat
     3661 tttccaaatt atcaacagct ttcgaggggc tttctataat cggaatagct cctcagtctg
     3721 tttgagttta tggatagatc cacaaataat taccattttc cttttcttga ttttaatttt
     3781 gatttttgtt ctaatctctg cctcttgttt ttacaagtct attaaggatc cgtagtagat
     3841 ttgaaataaa caagagtatc tttgtattta ttagaaggaa accaaaaggt gaaaaataga
     3901 aattattttg ttgcaatagc aacaatatta gccttatcgc cagaggttaa cgcaactgaa
     3961 ctttcagatc agcttgcaag tagaacaaga tattatcctt ccgccacgga tagttattat
     4021 agttactata aatatgcacc gatttattcg ggctactata catcgaatag ccgtaatggt
     4081 tattcgtcgt ggtattatcc aggtagcaat gcaggtgtaa caaccagaac tgtttcgaca
     4141 acagttaatg gcattacagt tacaaaagtt tatacaacgc ataatgggag aacaaccgtt
     4201 cgtaccactt atgctcctgc tgtgaacaaa ccaattgtaa ataaaccgac accgattgcc
     4261 aataaacccg ttgtagatac gccaactcaa ccggttattg atgccaaccc aattatggat
     4321 ccgccagtcc aaccaatagt tgataataaa cccatcactg atacacagcc taatccaatt
     4381 acagataaca aacctgtcat tgaatcgcca attgatgaca cccgtccagt tatcattagg
     4441 cctacaacac cggtaaatct tcatcctgaa cctgtagctg ttggtgtacc caatgtaggt
     4501 attcgatgga atgaccgctc tcgtggttac aacattgatg atcctaataa caaacaaaat
     4561 gttaagttaa ctggtgaagg tgttaaagtc ggtatcttag atagtggttt taaaaactat
     4621 ttaatgcagg acgatatact caaaaaattt ggtaatcgcg ctgttattat tgaaacaggg
     4681 cgtactgccc cggcttctgc aacacatggt attgaggtcg cagaaatggt tgggggaggg
     4741 aactctcgca atggagtagc gtctaagtca acgttattac ttgcggatat taccaaaaca
     4801 acaaatgcgg gtacaggatt atccgcaaca gctccaattt attatgactt atggaatagg
     4861 ggagcccgta tttttaacca atcatacggt attccaaagc ctcttactta ttttaataac
     4921 aatagaaatt ctagctatta ctatttacac caatttgatg gtaatgtgtt gcaattctat
     4981 aaggataaag taaaagaggg gggcttattt gtctgggctg cggggaataa ccgtggtgat
     5041 cttgatccat cactccaagc tgggttacct cattatgaag ctgacttgga aaaaggttgg
     5101 atttctgttg ttgcggtagc aaccagaact aatcagggat tgggtaactt tgaatggagc
     5161 aatttattgc catattctca agccggtgtg gcaaaaaatt ggaccgttac cgcaatgggt
     5221 gactacatct ttaatttagg aggtagaaac attattgcta gtggttcatc ctttgcagct
     5281 ccagccgtta cgggtacagc agctctcgtg aaacaaaaat atccttggat ggatggtaac
     5341 cttatcaaac aatctattct ctcgacggca accgatatcg gcgctcctgg agttgatgat
     5401 gtatatggat ggggtttact taatattgaa aaagccacta atggccctgc gcgttttggt
     5461 aaacgactcg ctttagccga taacgtgact gtaaatattc caaatggaag ctatgagttt
     5521 tccaataata ttaacggtga tgcaggcttg gtgaaaaatg gccaaggcga attaatttta
     5581 tcgggtgcta gtacttttga aggggataca acactcaatg aaggtgctgt cactattaac
     5641 ggtaaaaaat atgtgtctaa agtaaacgta tctccaagtg gtacattggt tgtcaataat
     5701 accacgttag aaaatggcgt aaataacgag ggtacagttg tcaaccaggg ttattctacg
     5761 attaagagag cttacgttgc ttcagcacaa agtacgctag tgtcagattt aggttcaaac
     5821 ttaaatgtaa aaggagaagt tgacttaaac ggctctactt tatcactagc tgcggtaaaa
     5881 gagggtaaag ctcagtatgt gacccaaaaa ggtttatcaa tgagtgctat tacttctgat
     5941 tccgcaatta agggtaactt tgctgcaata gaaacctcag ggttattaac tgcgacggcg
     6001 gaaaaaacca atgagaatga agtaaaagca acagttagcc ggaaaaatgt cgcgactgct
     6061 ttagagagct cattttcggc attggataac caaattgaag gaaataatgg ttctacaaat
     6121 gctacaaatg atgaattcgc acaacaagcg gctactttac aaaacagtat tgcaaacatc
     6181 aatacgcaaa gtgtggtttt agatagctta tctggtcaaa tttatgcgtc tgcgcaagcg
     6241 cttacttttg aaaactcaga aacagtgaat aaagatctct ctaaccgtct tgtaatgtta
     6301 ggaacgctgg ataatgtagg tgagactgct ggtgtgtgag taacgggtat ttatgggaat
     6361 ggtactctaa aagaaatagg gtttggtgaa ggtaaaacca atacctatgc tggccaaata
     6421 ggttttgata agccagttac agaccatgtg attttaggtg cggcacttaa ttattctaaa
     6481 gcaaaagtga acttcgatcg ttatggcggt agttcaaaag cagatggaat aggtgcatca
     6541 ttatatgccc gtataggcaa taagcataaa acaccttggt atttacaagg tcgagccggt
     6601 tatggcagtg ttgattcaga tgtagagcgt catattattt tggcggataa taacgcaagt
     6661 acagcaaaaa tcaaccaccg agacagagta ttttctgctt atgttgaatc aggctatgac
     6721 ttcaagcaag gtcgctttgc gttaactcca tttgtcggtt ttagtcatga tgttgtcaga
     6781 cgtggcgcat tcagtgagca aaacagtcaa tttggtctca ccgcggacaa agcaacctat
     6841 aaacaaacaa gcggattgat tggtttacga acatctttag aggtgaattt agctgggatg
     6901 aaaacgactt tccaaggcta tgtaaatcat caaaaagcgt ttaatcgaga agatcttagc
     6961 tttaaagcaa gttatacagg tttacaagat gcaaaattcg acgtaaaagg tattgggctt
     7021 gcaaaacata aaacgtgggt agggattggt gcattggcgg aagtaaataa aaatacttcc
     7081 ttatatgtga attacgacct taaactcgca aaaaatagtg gtcacaacaa cgttgtgact
     7141 gctgggatta gaattaattt ctaactgaat ctttttaaac aaaacgggcg tttatacgcc
//
LOCUS       DANLXS010000122         7140 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.122, whole genome shotgun sequence.
ACCESSION   DANLXS010000122 DANLXS010000000
VERSION     DANLXS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.122"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            39..944
                     /locus_tag="PXD71_001875"
     CDS             39..944
                     /locus_tag="PXD71_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL6291965.1"
                     /translation="MSQKYSVAVIGLGAMGMGAAKSCIAAGLDTYGVDLNPNYLETLK
                     AAGAKKVGTSAVDFAAELDAVLLLVVNANQVNAVLFGENGLAQHLKQGTVVMVSSTIS
                     AQDAKNISQKLTDLGLVMLDAPVSGGAAKAAQGEMTVMASGSSEAFEKLKPVLDAVAG
                     KVYNIGEEVGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTNAAGNS
                     WMFENRMKHVVEGDYTPLSMVDIFVKDLGLVNDTAKSLRFPLHLASTAYSMFTEASNA
                     GYGKEDDSAVIKIFSGIELPKKEGK"
     gene            946..2187
                     /locus_tag="PXD71_001876"
     CDS             946..2187
                     /locus_tag="PXD71_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL6291966.1"
                     /translation="MLGIIADDFTGASDIASFLTENGLKTVQMNGVPKTTLNSQVDAI
                     VISLKSRSNPVEEAIQQSLQALDWLKQNGCSQFYFKYCSTFDSTEKGNIGPVTDALMA
                     ALDTDFTVVTPALPVNGRTIFNGYLFVGDVLLNESGMRNHPITPMKDANLVRLMDAQS
                     QGKTGLVAYADVIQGAERVKARFAELKQQGYKYAVVDAADNSQLAVLAEAVADFQLVT
                     GGSGLGAYMAAYRSGGKKGTNAFVPSKGKTVVLSGSCSVMTNTQVAAYQQKAPSYFLE
                     AEKAIQDPNYVETLYQWVIANLDGDFAPMVYATVPPEKLKEIQTQFGGDKASHAIENT
                     FAKLAEKLQAAGVTNFITAGGETSSIVVQQLGFSGFHIGKQIAPGVPWLQAVEKPIFL
                     ALKSGNFGKEDFFSFAQEMFV"
     gene            2184..2816
                     /locus_tag="PXD71_001877"
     CDS             2184..2816
                     /locus_tag="PXD71_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDL6291967.1"
                     /translation="MTEQEQKIEMVNLARSLFERGYTVGGAGNLSVRLDENRILVTPT
                     GSSLGRLEADRLSVLDMQGNVLSGDKPSKESVFHLAMYQKNPACQAIVHLHSTYLTAL
                     SCLENLDTDNAMRAFTPYYVMRVGKLPVIPYYRPGDINIARELGERALDGKAFLLANH
                     GVVVTGSDLRDAVDNTEELEETSKLYFILQGQKVRYLTDDEVNDLKNRGK"
     gene            2818..3594
                     /locus_tag="PXD71_001878"
     CDS             2818..3594
                     /locus_tag="PXD71_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HDL6291968.1"
                     /translation="MPKFAANLTMMFNEVPFLDRFEAAANAGFKYVEYLWPYDYPAAE
                     LKAKLEQYGLKQVLFNTAAGDVAQGEWGVSAIPGREADSHRDIDLALEYALALGCENV
                     HVMAAVVPEGEDREAYKQTFIKNVQYAADKFKPHGINVLLEALSPEVKPNYLLKSQFD
                     TLEIVEAVNRDNVFVQLDYFHAQNVDGNLSRLTDKLKGKFAHIQIASVPDRHEPDEGE
                     INYQYIFNKLDEIGYEGYVGCEYKPRAETTSGLGWFEQYK"
     gene            3647..5005
                     /locus_tag="PXD71_001879"
     CDS             3647..5005
                     /locus_tag="PXD71_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntT protein"
                     /protein_id="HDL6291969.1"
                     /translation="MSNEMLILIGLASVVALLVIMIKGKVHPFLALGLVSIAVALTAG
                     IPMSKVIPTLISGMGSTLGSVALIVGLGAMLGKIIEKSHGADVLANWLLQRFGEKNAP
                     FALGMTGFIFGIPVFVDVGFIVLLPIIFSVARRLGGNILVYSLPIGLAMLLVHVLVPP
                     HPGIVAGAQNLNADIGFVLGIGLVAALPAYLIGQKFIPFFTKNNFVKVVTSTDLLQHQ
                     KDLIKEKGIELPSFGKVLAMISFPLILIMLGTVTATTLPKEHIVREVFSMVGSSPFAL
                     FVAVCLSAYVLGFRYGWTKHKLEEVLESALAPIAGIILITGAGGMFGKVLDASGVGKA
                     LADVLANTGLPLLVLSFILSALLRAAQGSATVATITTTTILAPAAVAAGYNDLQLALL
                     TAAIGAGSMTLSHVNDSLFWVWTKFFNVPVSVGLRTWTFLTTIFGVIAFAVVGIIWML
                     FV"
     gene            5067..6407
                     /locus_tag="PXD71_001880"
     CDS             5067..6407
                     /locus_tag="PXD71_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HDL6291970.1"
                     /translation="MLKKTLLFTLLLSHSLYAEDMIQENKSANITLLAEITAQGQKIN
                     GVALEYPDNVLSGSDLRQLYQVKTALDSAEKENRSVLKAYVNNEPKVTALPQAGKFVI
                     IELDTADKNAATYSVKDANKAPMKFKSKDENGNIVETEKVQAVKIPEFFANRLIYSVE
                     QKGLLKLTSGTTLGKTEWTQTAEPDKIKTPYLDDFVAKQVSLDQSGNKLNYRLYSPLQ
                     KNGEKYPLVIFLHGSGQVGSDNLAHLLSSKGAIATLPYAPSFVLAPQYDQVFDPFDNA
                     AKGQKGGIHWQTENRINLVLKMIDETLKANPQIDTTRIYINGLSRGAEGGLNLLLARP
                     NFFAGALLMSGREAYTNEWVDGNATKTNLAAIKHIPIWFFHSKEDKVSPVKGSRINYR
                     ILKDELNAPNVKYTEFTFEQAGDNGIVNNNPHNTWDAVYNSPEVIGWLLQQKRH"
     gene            6419..7108
                     /locus_tag="PXD71_001881"
     CDS             6419..7108
                     /locus_tag="PXD71_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005577085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDL6291971.1"
                     /translation="MFMYLSHPLDPKDLAWPGEPVVEVTRCTDVGEETPFSSFITKLP
                     NHCGTHMDAPRHFVKDGLSINELPIEYFCHKEVALIEIPKGEAEGITKEDLLPYVDIL
                     SKVSFAFLRTGFEKYRTENPAIYQNECPYISTGAGQYLSDTFPNLKGVGIDFLALGSP
                     SSRVPDAENPKNCHRAILGYYTGRFTTVIEDMHLAELPKNANIKQFFNAPWRIKGLDS
                     SQVTCIVELQQ"
BASE COUNT         2054 a         1457 c         1654 g         1975 t
ORIGIN      
        1 cgccatcaag ttcattaatt tatttgaggg aataaacaat gagtcaaaaa tatagcgtag
       61 ccgtcatcgg cttaggagca atgggtatgg gagcagcaaa atcttgtatc gctgccggat
      121 tagacacata cggtgtggat cttaacccga attatctgga aaccttaaaa gcagcaggtg
      181 ctaagaaagt cggcacaagt gcggtcgatt ttgccgctga attagatgcg gtgttgttgt
      241 tggtggttaa tgccaaccaa gtgaatgcgg tgttattcgg tgaaaacggc ttagcccaac
      301 atttgaaaca aggtacggtg gtgatggtat cttccaccat ttctgcacaa gatgcaaaaa
      361 atatttcaca gaagttaaca gatttaggct tggtgatgtt agatgctccg gtttcgggtg
      421 gggcagcaaa agcagcacaa ggtgaaatga ctgtgatggc atcaggttcc agcgaggcat
      481 tcgagaaatt aaaaccggta ttagatgcgg ttgcaggcaa agtgtataac attggcgaag
      541 aagtcggctt gggtgcaacg gtaaaaatta tccatcaatt acttgccggt gtgcatattg
      601 ccgcaggtgc agaagcaatg gcattggcgg ctcgtgccgg tattccgctt gatttaatgt
      661 atgacgttgt aaccaatgcg gcaggcaatt cttggatgtt tgaaaaccga atgaaacacg
      721 ttgttgaggg cgattacacc ccactctcta tggtggatat ttttgtgaaa gatttgggat
      781 tggtgaacga caccgcaaaa tctttgcgtt tcccgcttca cttagccagc accgcttatt
      841 ctatgtttac cgaagccagc aacgcaggtt acggcaagga agatgatagt gcggtcatta
      901 aaattttcag tggtatcgaa ttacctaaaa aagaggggaa ataagatgtt aggcattatt
      961 gcagacgatt tcaccggtgc gagtgacatc gccagtttct taaccgaaaa cgggctaaaa
     1021 acggtgcaaa tgaatggtgt gccgaaaact acacttaaca gtcaagttga tgcgattgtg
     1081 attagcctga aatctcgctc taaccctgtg gaagaagcaa tccaacaatc attacaagcc
     1141 ttagattggc tgaaacaaaa tggttgctcg caattctact ttaaatattg ttccactttt
     1201 gacagcacgg agaaaggcaa tatcgggccg gtaaccgatg ccctaatggc ggcattagat
     1261 accgatttta ccgtagtcac accggctctg cctgtcaatg gcagaaccat tttcaacggc
     1321 tatttgtttg tgggcgatgt gctactgaat gaatctggta tgcgtaacca cccgattacc
     1381 ccgatgaaag atgccaactt ggtgcgttta atggatgcac aatctcaagg caaaaccggc
     1441 ttggttgcct atgctgatgt gattcaaggg gcagagcgtg tgaaagcccg ttttgctgaa
     1501 ttgaaacagc aaggctacaa atatgcggta gtagatgctg ccgataattc acaacttgcg
     1561 gtgcttgccg aagcggttgc cgattttcaa ttagtcacag gcggttcagg cttaggggct
     1621 tatatggcgg cttaccgttc aggcggcaaa aaaggcacga atgcgtttgt accaagcaaa
     1681 ggcaaaaccg ttgtgctttc gggttcttgc tcggtaatga ccaataccca agtggcggct
     1741 taccagcaaa aagccccaag ttatttctta gaagcggaaa aagccattca ggatccgaac
     1801 tatgtagaaa cgctttatca atgggtgatt gcaaacctag acggtgactt tgccccaatg
     1861 gtttatgcca ccgttccccc tgaaaaattg aaagagattc aaacccaatt cggcggcgat
     1921 aaagccagcc acgccattga aaataccttt gccaaattag ccgaaaaatt acaagccgca
     1981 ggcgtaacta actttattac cgcaggcggt gaaacttcga gcattgtggt tcagcaactt
     2041 ggattcagcg gtttccacat cggcaaacaa attgcaccgg gcgtgccgtg gttacaagcg
     2101 gtcgaaaaac cgatttttct tgcattgaaa tcgggtaact tcggtaaaga agatttcttc
     2161 agttttgctc aggagatgtt tgtatgacag aacaagaaca aaagattgaa atggtcaatc
     2221 ttgcccgctc gctgtttgag cggggctata ccgttggcgg tgcgggcaac ctctccgtgc
     2281 gtttagatga aaatcgcatt ttagttaccc caaccggctc ttcgctcggg cgtttagagg
     2341 cagaccgcct ttccgtattg gatatgcagg gtaatgttct ttcaggagat aaaccttcaa
     2401 aagaatcggt attccacttg gcgatgtatc agaaaaatcc ggcttgccaa gcaatagtgc
     2461 atctgcattc cacttattta accgcacttt cttgcttgga aaatttggat accgacaacg
     2521 ctatgcgtgc tttcacccct tactatgtga tgcgggtagg aaaattgccg gtcattcctt
     2581 actaccgtcc gggcgacatc aacattgccc gagagctggg ggaacgagcc ttagacggca
     2641 aagccttttt actggcaaat cacggggtgg tagtcacagg ttcagacttg cgtgatgcgg
     2701 tggataacac agaagagttg gaagaaacct ccaagctcta ttttattcta caaggtcaaa
     2761 aagtccgtta tctcaccgat gacgaagtca atgatctgaa aaacagagga aaataaaatg
     2821 cctaaatttg cagcaaacct tacgatgatg tttaacgaag tgccgttttt agatcgtttt
     2881 gaagcggctg ccaacgctgg atttaaatat gtcgagtatt tatggcctta tgattatccg
     2941 gcagcagaat tgaaagccaa acttgagcaa tatggcttaa aacaggtgtt gttcaatacc
     3001 gcagccggag atgttgctca aggtgaatgg ggtgtttctg ccattccggg gcgtgaagct
     3061 gatagccatc gtgatattga tttagcgttg gaatatgccc tcgccttagg ttgtgagaat
     3121 gttcacgtta tggcagcggt cgtgccagaa ggcgaagatc gtgaagccta taaacaaacc
     3181 tttatcaaaa acgtgcaata tgccgccgat aaattcaaac cgcacggcat taacgtcttg
     3241 cttgaagccc taagccctga agtgaaaccg aattatctgc ttaaaagcca gtttgatacg
     3301 ctagaaattg tggaagcggt aaaccgagac aatgtgtttg tacagcttga ctacttccac
     3361 gcacagaatg tggacggcaa cttatctcgc ctaaccgaca aactcaaggg taaatttgcc
     3421 cacattcaaa ttgcctctgt gccagatcgc cacgaaccgg acgaagggga aattaactac
     3481 caatatatct tcaacaaact cgatgaaatc gggtatgaag gctatgtagg ttgcgagtat
     3541 aaaccacgag cggaaaccac ctcaggacta ggttggtttg agcagtataa ataaccacga
     3601 tgattagcgg ataaatacgt gaatacaaca tttagaagga aactttatgt caaatgaaat
     3661 gttaattttg attggattag caagcgtagt tgctcttctg gtcattatga ttaaaggtaa
     3721 agtacaccca ttcttagcat tggggttagt gagtatcgca gttgccttaa ctgcaggtat
     3781 tccaatgagt aaagtgatcc caaccttgat ttcaggtatg ggaagtactt taggtagcgt
     3841 tgcactcata gtaggtttgg gagcaatgtt agggaaaatc attgaaaaat ctcacggtgc
     3901 ggatgtatta gcaaattggt tgttacagcg ttttggtgag aaaaacgctc cctttgcatt
     3961 aggtatgaca ggttttatat tcggtattcc tgttttcgtt gatgttggtt ttatcgtatt
     4021 gctcccgatt atttttagtg tagcgagacg tttaggggga aatatccttg tttattcttt
     4081 gccaatcggg cttgcgatgt tacttgttca cgttttagtt ccacctcatc caggtattgt
     4141 agcgggtgct caaaacttaa acgcagatat tggctttgta ttgggaattg gcttagtagc
     4201 agcgctacct gcatatttaa tcggtcaaaa atttattccg ttctttacaa aaaataattt
     4261 cgtaaaagta gtaacttcaa ccgatctttt acagcaccaa aaagatttga ttaaagagaa
     4321 aggcattgaa ctaccttctt ttggtaaagt gttagcaatg atttctttcc cgctaatcct
     4381 gattatgtta ggtacagtaa cggcaacaac cttgccgaaa gaacatatcg tgcgtgaagt
     4441 attcagtatg gtaggaagct ctccgtttgc cttatttgtg gctgtttgtc tttctgctta
     4501 tgtattaggt tttagatacg gttggactaa gcataaatta gaagaagtac tggagtctgc
     4561 gttagcaccg attgcgggga ttattttaat caccggtgca ggcggtatgt tcggtaaagt
     4621 gttagatgcc agtggcgtag gtaaagcatt agcagatgta ttagccaaca cgggcttacc
     4681 gttattggtt ctttctttca tactttctgc attattgcgt gcagcacaag gttctgcaac
     4741 ggtggcaact attactacta caactattct agctcctgca gctgtagcag caggttataa
     4801 tgatctgcaa ttagcacttc ttactgcagc tattggtgca ggttctatga cattatccca
     4861 cgtgaatgat tcactcttct gggtatggac gaaattcttc aatgttcctg tatccgtagg
     4921 cttaagaact tggacgtttt taacgactat tttcggtgta atagcctttg ccgttgttgg
     4981 cattatttgg atgttattcg tttaatatca ctctgttact gcttaaaatc cacctcttat
     5041 gaggtggatt tttttaggag aaacctatgc tgaaaaaaac acttttattt acgctattac
     5101 tttctcactc cctctatgca gaggatatga ttcaggaaaa taaatcagcg aatattacgt
     5161 tgcttgccga aatcaccgct caaggtcaaa aaattaacgg cgtggcattg gaatatcctg
     5221 ataatgtctt atcaggcagc gatcttcgcc aactgtatca ggtcaaaacg gcattagata
     5281 gtgcggagaa agaaaaccgt tcggtgctca aagcctatgt caataatgag ccaaaagtga
     5341 ccgcacttcc gcaagccggt aaatttgtga ttattgaatt ggatacggca gataaaaatg
     5401 cggcaactta tagcgtaaaa gatgctaata aagcgccgat gaaatttaaa tccaaagatg
     5461 aaaacggcaa cattgtagaa acagagaaag tacaagcggt caaaattcca gagttttttg
     5521 caaatcgcct aatctattcg gtggaacaaa aaggcttgct taaattaacc agcggcacaa
     5581 ctctaggcaa gactgaatgg acacaaaccg ctgaacccga caaaatcaaa accccttatc
     5641 tcgatgattt tgtcgcaaaa caagtgagct tagaccagtc tggaaataaa ctgaactacc
     5701 gtctctattc gcctttgcaa aaaaatggtg aaaaataccc gcttgtgatt ttcctacacg
     5761 gttcagggca agtcggttcg gataaccttg ctcacctgct ttccagtaaa ggggcgattg
     5821 ccaccctgcc ttatgcccca agttttgtac ttgctccgca atacgaccaa gtattcgacc
     5881 catttgataa tgccgccaaa gggcaaaaag gcggtattca ctggcaaacg gaaaatcgta
     5941 tcaatctcgt gttaaaaatg attgatgaaa ccctaaaagc caatcctcaa attgatacca
     6001 cacgcattta tatcaatggc ttatctcgag gtgctgaagg gggcttaaat ctgttgttgg
     6061 ctcgcccgaa tttctttgcc ggagcattat taatgagcgg tcgtgaagcc tacaccaacg
     6121 aatgggtgga cggcaatgcc accaaaacga atttggcggc aattaaacat ataccgattt
     6181 ggtttttcca tagtaaagag gacaaagtct cgccggtaaa aggttctcgc attaactacc
     6241 gcattctcaa agatgagtta aatgccccca atgtgaaata taccgaattc acttttgaac
     6301 aagccggcga caacggcatt gtcaataata accctcataa tacttgggac gctgtgtata
     6361 acagccctga ggtgattggg tggttacttc agcaaaaacg ccactaagga ggctcactgt
     6421 gtttatgtat ttgtctcacc cgttagatcc taaagattta gcttggccgg gagaacctgt
     6481 tgttgaggtg actcgctgca cagacgtagg tgaagaaacg ccatttagca gttttatcac
     6541 gaaactaccg aatcattgcg gcacacatat ggacgctcct cgccactttg tgaaagacgg
     6601 tttaagtatc aatgaattgc cgatagagta tttctgccat aaagaggtcg ctttaattga
     6661 aattcccaaa ggagaggcag aagggatcac caaagaagat ttattgcctt atgtcgatat
     6721 tctctccaag gtgtcttttg cctttttacg aacggggttt gagaaatata gaaccgagaa
     6781 ccctgctatt tatcaaaatg aatgccccta tatttcaacc ggtgccgggc agtatttatc
     6841 tgatactttc ccgaatttga aaggagtagg cattgatttc ttggcactag ggtctccttc
     6901 ttcccgagtg cctgatgcgg aaaaccctaa aaattgccat cgagcgattt taggctacta
     6961 taccggcagg tttaccactg tgattgaaga tatgcactta gcggaactcc ctaaaaatgc
     7021 caacattaag cagtttttta atgcaccgtg gcgaattaaa gggttagatt ccagtcaagt
     7081 gacttgtatt gtggaattac agcaataaac cacagatata ttcattttag gagaggctat
//
LOCUS       DANLXS010000123         7096 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.123, whole genome shotgun sequence.
ACCESSION   DANLXS010000123 DANLXS010000000
VERSION     DANLXS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.123"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1299)
                     /locus_tag="PXD71_001882"
     CDS             complement(<1..1299)
                     /locus_tag="PXD71_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL6291972.1"
                     /translation="MQEAYDIQHLCTRKEDKSFDRKSARKEPKEILRHLIGFANADGG
                     LLVIGIEDNGEITGFNYQKAHQPEEFIHALHNLQKMPIPVTYQQIEVVNSRGLADFVL
                     LFEVTASSGRVITSHDDRVFLRSKDQTLELNFEQRLQLEYEKGQRAFEEQPIARADFT
                     DLDMDLLEQYRQKLHTDRSIEEILKARDLINKEGQITNACILLFGNNPTRYFPNARLR
                     FLRYEGKAPLTGRTFNVVKEFMLEEPIPKLLESAKAVLKSQLREFQRLDENGQFTKID
                     EYPEFAWLEGIVNAIAHRSYSNQGDCIRISMFDDRLEIFSPGGLPRPVTLENMYYTRF
                     SRNPKIARVLYEFGWVKELNEGVNRMAEEMNAYQLNTPSYFEPNENAVRLILENNIEH
                     RTLRALERIEEIFTQDIMATLNKNEVTILQYLYENEMITVK"
     gene            complement(1399..1803)
                     /locus_tag="PXD71_001883"
     CDS             complement(1399..1803)
                     /locus_tag="PXD71_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL6291973.1"
                     /translation="MHKFTFPIEQIAPLVPHSGNMILLDRITAFGEDFLTAETNIRPD
                     NPLIKHGKLATYASIEIMAQAGEPVRLGYLLGTRKLHLHRQEIPIGSQLQIQIKMSIQ
                     DATGFGVFDCQLIDLADNQVLLEGALNVFSPK"
     gene            complement(1796..3018)
                     /locus_tag="PXD71_001884"
                     /pseudo
     CDS             complement(1796..3018)
                     /locus_tag="PXD71_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            complement(3105..3524)
                     /locus_tag="PXD71_001885"
     CDS             complement(3105..3524)
                     /locus_tag="PXD71_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291974.1"
                     /translation="MLRLLSFCFIFLLSACTVLPQIKQLPPQVESVKSFKVENNGQQS
                     LLLIQFYENKWRWIQTDPLGAPIARVWLTKKGWEPDGFVMPNKQAQLLFTALVNALNP
                     HSPPFYLDKGWKIEREKSQFTIWLPDSSKWKVEILGE"
     gene            complement(3586..4275)
                     /gene="rplA"
                     /locus_tag="PXD71_001886"
     CDS             complement(3586..4275)
                     /gene="rplA"
                     /locus_tag="PXD71_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL6291975.1"
                     /translation="MAKLTKKMKAIKAGVDSTKAYDINEAVEVLKKFATAKFVESVDV
                     AVNLGIDSRKSDQNVRGATVLPHGTGRTARVAVFTQGANAEAAKAAGADLVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKSGQ
                     IRYRNDKNGIIHTTIGKADFSAEQLKDNLNALLAALTKAKPTTAKGIFIKKVSISTTM
                     GAGVAVDQASL"
     gene            complement(4280..4708)
                     /gene="rplK"
                     /locus_tag="PXD71_001887"
     CDS             complement(4280..4708)
                     /gene="rplK"
                     /locus_tag="PXD71_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL6291976.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     RTESLEKGLPIPVVITVYADKSFTFVTKTPPAAVLLKKAVGIKSGSGKPNKDKVGTVT
                     QEQIRQIAETKAADMTGATIETKMKSIAGTARSMGLIVEE"
     gene            complement(4941..5504)
                     /gene="nusG"
                     /locus_tag="PXD71_001888"
     CDS             complement(4941..5504)
                     /gene="nusG"
                     /locus_tag="PXD71_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL6291977.1"
                     /translation="MSEVEVKENGSKRWYVLQAFSGFENRVAVTLREYIKLHKMEEQF
                     GEVLVPTEEVVENVGGKRRKTERKFFPGYVLVEMEMNDDTWHLVKSVPRVMGFIGGTA
                     DRPAPITKREADRILNRVQETAEKPRHRKEFQPGESILVTEGPFADFSGTVEEVDYEK
                     GRLKVSVSIFGRATPVELEFSQVEKQS"
     gene            complement(5506..5919)
                     /gene="secE"
                     /locus_tag="PXD71_001889"
     CDS             complement(5506..5919)
                     /gene="secE"
                     /locus_tag="PXD71_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL6291978.1"
                     /translation="MATEIKTKTPEQVEQKGGKSKGANTALWLVAIVFLAVAAVGNAY
                     FSSHFSLIVRVLLLVVLLVGAVALAAITNQGQKAIHFIKESRTELRKIIWPTRPEATQ
                     TTLIVLAMCVVVSLVLWGIDSIIVALITFVTSLRF"
     gene            complement(6032..7054)
                     /locus_tag="PXD71_001890"
     CDS             complement(6032..7054)
                     /locus_tag="PXD71_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6291979.1"
                     /translation="MANGTSRNRRSSGRVTLADVAKVVGVGQMTVSRALRTPEMVSEP
                     LRKRIEEAIIELGYIPHFSARQLASAESHNLVIVTTSISSTENTLILSALRKSVADSA
                     LQMVILIADQESWFQELINHAPQAIVFLNLDCPPNVREWIKNSNIPTVEIGAIKKEPI
                     HLSVGIDSHEAMEKMIHFLVKKGYREIGLLSAKQDLSIFKQYLESWHTTLFAHHISPL
                     KIHHVVEDISFSAGATLLTEALLNWEAIDALVFLSDELACGALYEALRRHMSIPKDLA
                     IAGLGGLEVGSISYPKLTTIAIPYEQMGKIAGKQLLTLLSGKTLSAEEMIVKLPNKLV
                     IRDSAA"
BASE COUNT         1955 a         1582 c         1380 g         2179 t
ORIGIN      
        1 ctttacagta atcatttcat tttcatataa atattgcagg atagtaactt catttttatt
       61 taatgtcgcc ataatatcct gcgtaaaaat ttcttctatt cgttctaatg cccttaatgt
      121 ccgatgttca atattatttt ctaaaatcag tctgaccgca ttttcattgg gttcaaagta
      181 agaaggtgta ttaagttggt aagcgttcat ttcctccgcc atacgattaa caccttcatt
      241 cagctctttg acccaaccaa attcatacag cactcgtgca atcttcggat tgcgtgaaaa
      301 acgggtatag tacatatttt caagcgtcac agggcgaggt aatccacccg gactaaaaat
      361 ttccaaacgg tcatcaaaca tcgaaatgcg aatgcagtcc ccttgattac tgtaactacg
      421 gtgtgcaatc gcattgacaa taccttctaa ccaagcaaat tctggatact catcaatttt
      481 tgtgaattgc ccgttttcat ctaaacgttg gaactctcgt aattggcttt ttaatactgc
      541 cttagcactc tccaataact taggaatagg ctcttcaagc ataaattctt tgacgacatt
      601 aaaagttcta ccggtcagag gtgctttacc ttcataccgc aaaaaacgca aacgggcatt
      661 aggaaaataa cgtgtcgggt tattcccaaa tagcaaaata caggcattcg taatttgccc
      721 ttctttgtta attaaatctc gggctttcag aatctcttct attgagcgat ctgtgtgtaa
      781 tttctgtcga tactgttcta ataaatccat atccagatca gtaaaatcag cgcgtgctat
      841 cggttgctct tcaaaagcac gctgcccttt ttcgtactct aattgcaaac gttgttcaaa
      901 attgagttcc aaagtttgat cttttgagcg tagaaaaacc cgatcatcgt gagaggtaat
      961 caccctccca ctcgaagctg taacttcaaa taataagaca aaatccgcta aacctcgact
     1021 atttaccacc tcaatttgct gataggtcac cggtatcggc attttttgta aattgtgcag
     1081 agcgtggata aattcttccg gctgatgggc tttttggtag ttaaaaccgg taatttcgcc
     1141 attatcttca ataccaataa ctaataaacc gccgtctgca ttcgcaaaac caatcaaatg
     1201 ccgcaaaatc tctttcggtt cttttctcgc acttttgcga tcaaaggatt tatcttcttt
     1261 gcgagtacaa agatgttgaa tgtcataggc ctcttgcata acgaatttcc taccaactac
     1321 taccaactac taccaactac taccaactac tacttactac tacttactac tacttactac
     1381 tacttactac taccaactct atttcggact aaacacattc agcgcccctt ctaaaagcac
     1441 ttggttgtcg gctaaatcga tcagttggca gtcgaatacg ccgaagccgg tggcatcttg
     1501 aatagacatt ttgatctgaa tttgtagctg tgagccaatc gggatttctt ggcggtggag
     1561 gtgcagtttg cgagtgccga gcaggtagcc taagcgaact ggttcgccgg cttgtgccat
     1621 aatttcaatg ctggcgtagg tggcaagttt tccgtgtttg atcagcggat tgtcagggcg
     1681 aatattggtt tcagcggtca aaaaatcctc gccgaatgcg gtaatgcggt cgagtaggat
     1741 catattgcca ctgtgaggaa ccaatggtgc aatttgttcg atagggaagg tgaatttatg
     1801 catcgctttc tcccaaaatc agtacgctgt tattgccgcc aaaggcaaaa gagctgcttg
     1861 cgccaatccg tctgcctgct gcccaacggc tctgttcatc ggtgatcgca atgttcggca
     1921 ggttttcgtc cgcttgttta tcccataatt gcgcagggag cttgccggtc gggttgtgtt
     1981 ggcgacagag cattccccac aaaattgccg cttccaccgc accggcagcc caagggtatg
     2041 cccggtgtag ggtttggtgg tggtgcaagc ggtctgatta ccgaagattt ttgccacagc
     2101 gaggctttcc atttgatcgt tatgaagcgt ccctgtgccg tgcaggttga tccagccgat
     2161 gtcggctgcg tctaaatcag cattgtgcag ggcattttgg aaagcggaaa ttgccccttc
     2221 cccttcgggg tgaggtgagg acatatggta agcgtccgag ctggaaccat aacccagtaa
     2281 ctgaatgcga tgttcgtcca gtggttcacg gctcatcaca aacaccgctg cgccttcgcc
     2341 gatgttaatg ccgttacggt tagcggaaaa tgggttggtt tgttggtttg ataacacttc
     2401 cagcgagcta aatccgctga tagttaaagg cgaaagggta tccacaccac cgcaaatcac
     2461 cgcatcgcac aagccggctt tcagtaaacg ggcagcagaa attaacgctc ttgcctctga
     2521 ggtgcaggcg gtggaaatgc cgtaactcag ccccgttaag ccatacactt cggcaacgaa
     2581 atcagacggc gcagagaaga gaatttgctg ttgattaaac tgctcggtgg cataatcgcc
     2641 ctgcacaccg gctttgaaga cgggaatatt ttcatcggcg ccggtagtgg acgtgccgat
     2701 gatcaccgca atccgctcac gcccgaattg ctcgatacaa gcggtaattt tcggctcaat
     2761 ttctgcaagg gcgtgccata gcagttggtt attgcggctg cggtgttcaa tagatagttc
     2821 aacaggaaaa tcacgcaatc tagcagagac ttgccctaga aatttcggtg aactatttga
     2881 aatattatgt cgtaaataag gctgtgaagt cgtatctaaa ggagatactt tcgcattaat
     2941 taaaacattc aaatgctcca cgatgccata tccaatcgga ctggtgatgg cgggctctga
     3001 taaatatagt gtcattatta tttattccag tattattgtg taatggcact aaaattataa
     3061 aattcatatt caagtgattc aagcatagag gttcaagttt tcgcttattc ccccaatatt
     3121 tccactttcc atttactgct atctggtagc caaatcgtaa attgggattt ttccctctca
     3181 attttccaac ctttatctaa ataaaaaggt ggagaatgag ggtttaatgc attgactaaa
     3241 gctgtaaata atagctgtgc ctgcttattt ggcattacaa agccatctgg ctcccaacct
     3301 tttttagtaa gccatactct ggcaatagga gcacctaaag gatcagtttg aatccaacgc
     3361 catttatttt cataaaattg aattagaagc aaactttgct ggccattatt ttcaacttta
     3421 aaggatttta cagattcaac ctgtggcggt aattgcttaa tttgaggtaa tacagtacaa
     3481 gcactaagca aaaaaataaa gcaaaaagat aataatctta acattcgtac ctaaagaaaa
     3541 agaccgtaga gtattaacac tacggtcttt tgattctatt aaaaattaaa gtgatgcttg
     3601 atcaaccgca acaccagcac ccattgtagt agagatgctt actttcttga tgaagatacc
     3661 ttttgctgtt gttggttttg cttttgttaa agcagctaac aatgcgttta agttatcttt
     3721 taattgttca gctgagaagt ctgctttacc gatagtagtg tggataatac cgtttttgtc
     3781 gttacggtag cggatctgac cagatttcgc atttttaacc gcttctgcaa cgtttggtgt
     3841 tactgtacct actttcgggt ttggcattaa gccgcgtgga cctaatactt gacctaattg
     3901 acctacaaca cgcattgcat ctggagaagc gataacaaca tcaaagttca tttcgccttt
     3961 cttgatttgc tctgcaagat cttccatacc cactaagtca gcacccgctg cttttgctgc
     4021 ttctgcgttt gcaccttgtg taaacacagc aacacgagcg gtacggcctg taccgtgtgg
     4081 taatacggtt gcaccacgca cgttttggtc tgatttacga gaatcgatac ctaagtttac
     4141 agcaacgtcc acgctctcaa cgaatttagc ggttgcgaat ttttttaaca cttcaactgc
     4201 ttcgttgatg tcataagctt tagtagaatc tacgccagct ttaattgctt tcattttttt
     4261 agtcaattta gccattgtat tattcctcta cgattaagcc cattgagcga gctgtaccag
     4321 cgattgattt cattttggtt tcgatagtcg caccagtcat atctgctgct ttagtttcag
     4381 cgatttgacg gatttgctct tgagttactg tacccacttt atctttgttc ggtttaccag
     4441 aaccagattt gatacctacc gcttttttca gtagcactgc cgccggtgga gttttggtaa
     4501 cgaatgtgaa agatttatca gcgtaaactg tgataacaac cggaatcggt aaacctttct
     4561 ctaagctctc agtacgagcg ttgaatgctt tacagaattc cataatgtta acaccttgtt
     4621 gacctaatgc aggaccaacc ggtggtgatg ggttagccat acccgctgca acttgcagtt
     4681 taacgtaggc ttggactttt tttgccattt taattttcct ctaaatgggt gataacgctg
     4741 aaaatcagct cccctgttac ataaaaaata cgagtcagca ggctggctcg taataggtcg
     4801 caaattttaa agaatttgcg actatttttc aagcaaaact ttcgctttgt tgaataaatc
     4861 ttgttacaag cggtcttttt tccgctgaat tttgcaaaaa attgagaaaa ataaccgctt
     4921 gtgtgtgaaa ttaattgaaa ttagctctgc ttttccactt gactgaattc taattctacc
     4981 ggtgtcgcac gaccaaaaat cgacaccgat acttttaagc gacctttttc gtaatccact
     5041 tcttcgaccg taccggagaa atctgcaaac ggaccttctg taaccaaaat gctttcgccc
     5101 ggttggaatt cttttctgtg gcgtggtttt tccgctgttt cttgtacacg gtttagaatg
     5161 cggtctgctt cacgtttggt aattggtgcg gggcgatctg ccgtaccacc aataaagccc
     5221 attacacgag gcacactttt cactaaatgc caagtatcgt cattcatttc catttcaacc
     5281 aatacataac ctgggaagaa tttacgctcg gttttacggc gtttaccgcc cacattttca
     5341 acgacttctt cggttggcac taatacttca ccaaactgct cttccatttt gtgaagctta
     5401 atgtattcac gcaatgttac tgccacacgg ttttcaaagc ctgaaaacgc ttgtaataca
     5461 taccagcgtt ttgagccatt ttctttcact tctacttcac tcattttaga accttaaact
     5521 tgttacaaat gtaattaatg cgacgatgat tgagtcaata ccccataaaa ccaaggaaac
     5581 tactacgcac attgccaata caatcaaggt ggtttgtgtt gcttcagggc gtgtcggcca
     5641 gataattttg cgtagttcgg ttcgagactc tttaataaaa tgaattgcct tttgcccctg
     5701 atttgtaatt gccgctagtg caacagcacc aactagcaac accaccaata aaagcactcg
     5761 aacaattaat gaaaaatgag atgaaaaata ggcattacct accgcagcaa ctgctaaaaa
     5821 tacaatcgca accagccata atgctgtatt tgcacctttg cttttccccc ctttttgttc
     5881 gacttgctcg ggggtttttg ttttaatctc agtagccata tataacctaa aaaatgaata
     5941 aaaaaatcag aaattatttt aaagcgttaa attctatcat tttctacaag acttgccacg
     6001 atcttttata gatttttatg caaaatcaat attatgcggc gctatcacga atcactagct
     6061 tattcggtaa tttaacaatc atctcttctg ctgataaggt tttaccactc aaaagagtaa
     6121 gtagttgttt acctgctatt tttcccattt gctcgtaagg gatcgcaatg gttgtcaatt
     6181 tagggtagga aatactcccc acttctaaac cacctaaacc ggcaatcgca agatcttttg
     6241 gaatgctcat atgtcggcgt aatgcttcat ataatgctcc gcaagccaat tcatcggata
     6301 aaaaaaccaa tgcatctatt gcttcccaat ttagaagggc ttcagtcaac aaagttgctc
     6361 ctgccgaaaa cgaaatatcc tctactacgt ggtggatttt taaaggagaa atatgatgtg
     6421 caaataaagt cgtatgccaa ctttctaaat attgtttgaa gatggataaa tcttgcttcg
     6481 ccgataacaa tccaatctct cgatagcctt ttttcaccaa aaaatggatc attttctcca
     6541 ttgcttcgtg gctatcaatt ccaacactga gatgaatcgg ttcttttttt atcgcaccta
     6601 tttctacggt agggatattg ctgtttttaa tccactcacg gacattagga gggcaatcta
     6661 aattcaaaaa cactatcgct tgaggagcat gattaatcag ttcttgaaac caagattctt
     6721 gatcagcgat caaaatcacc atttgcaatg cggaatccgc cacgcttttg cgtaatgccg
     6781 ataaaattaa ggtattttcc gttgagctaa tcgacgtcgt cacaatgacc aaattatgtg
     6841 attctgcaga agctaattgc cgagcagaaa aatgcggaat atatcctagt tcaatgattg
     6901 cctcttcaat ccgtttacgt agtggctccg acaccatttc tggtgtacgc aaagcacgag
     6961 ataccgtcat ctgcccaaca ccaacaactt tggctacatc agctaaagtg actctgcccg
     7021 aagatcttcg atttctactt gttccatttg ccatactatt ttcctaattg ttccatatta
     7081 tagggctaaa tcctta
//
LOCUS       DANLXS010000124         7096 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.124, whole genome shotgun sequence.
ACCESSION   DANLXS010000124 DANLXS010000000
VERSION     DANLXS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.124"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..288)
                     /locus_tag="PXD71_001891"
     CDS             complement(<1..288)
                     /locus_tag="PXD71_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6291980.1"
                     /translation="MTLETFNQQKAKFHEQEKAVLAIQTELDKAKNILEALQNEKAEF
                     VARQKTNLANIGTLSADEYVEIKNKNSGLQARIEYYQALIVDLENKCYAEQE"
     gene            complement(298..504)
                     /locus_tag="PXD71_001892"
     CDS             complement(298..504)
                     /locus_tag="PXD71_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDL6291981.1"
                     /translation="MENTPAKQRFMKIDEVSAVTTLSETQIYRKMKAGDFPQSISIGA
                     NSKVWLESEIQQWIQRKIEQHRNQ"
     gene            complement(974..2188)
                     /locus_tag="PXD71_001893"
     CDS             complement(974..2188)
                     /locus_tag="PXD71_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6291982.1"
                     /translation="MTATKKPLSDTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIY
                     SYISPKTGKRSTKNRIGSYPQMSLAEARLKRDELNTLVLQGLDPFEHLAKMQREAKEK
                     QESIQSFAYKWADWKLSKGKCKPKTMEKCLQRLENHLFPRFEGYTLEDFTLTDTINRL
                     TDLDRIMPDTLHRIAGNLIEILDYAVLCGRINFNPISVIKKAFSSVSTTHQPAIMPEE
                     LAEFMQDLQKSNRTPQIKLLVEWQLLNILRPFEAVAVQWSDIDWETKTLNIPADRMKG
                     GKNPHSVPLTKQAIAILEEMKKYNGHHKHIFTHRTDRNKPANSQTVNNAIKRLSGGKY
                     KGLLVAHGLRSIASTYLHERFTTETLVIEACLSHTNKDTVMSAYHRKNWLDRRRGIMQ
                     EWADFVEQCKSI"
     gene            complement(2391..2466)
                     /locus_tag="PXD71_001894"
     tRNA            complement(2391..2466)
                     /locus_tag="PXD71_001894"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2431..2433),aa:Ile,seq:cat)
     gene            complement(2560..3297)
                     /gene="pgeF"
                     /locus_tag="PXD71_001895"
     CDS             complement(2560..3297)
                     /gene="pgeF"
                     /locus_tag="PXD71_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDL6291983.1"
                     /translation="MDKIIPNWNVPKHIHALTTLRTGGVSKPPFDSFNLGDHVNDEPQ
                     DIAQNRALLVEKFQLPQAPLFLTQTHSTRVIELPYSGDNIKADAIYTNQPNQVCLVMT
                     ADCLPVLFASQDGSEIAAAHAGWRGLCDGILEATVEKFNCPPHEISAWLGPAIGPNAF
                     QVGSEVADQFCAFDPRAKEALIEDSTTSGKFLGNLYQIATQRLNKLGITAISGGEYCT
                     YSQPELFFSYRRDKQTGRMATLIWRTE"
     gene            complement(3329..3715)
                     /locus_tag="PXD71_001896"
     CDS             complement(3329..3715)
                     /locus_tag="PXD71_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL6291984.1"
                     /translation="MTVIHTENAPAAIGPYVQAVDLGNMVLTSGQIPVNPETGEIPSD
                     IVQQTRQSLNNVKAIIEQAGLTVADIVKTTVFVKDLNDFAKVNAEYQAFFQENEHPNF
                     PARSCVEVARLPKDVGIEIEAIAVRR"
     gene            3796..4797
                     /gene="yejK"
                     /locus_tag="PXD71_001897"
     CDS             3796..4797
                     /gene="yejK"
                     /locus_tag="PXD71_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDL6291985.1"
                     /translation="MSINVSEIVLHQLQKTEGENPELNTFLRENLLAISPEVDQMMLQ
                     LHQAYQSKAKAYGIFKPESVFAQQLNRLLEQETDFLPFSHSCAKMLAAELIKYPFADG
                     GTFILCRYTFLATEYLFIALIDSRNSMLVDEQLEIKRTQYLEIAQYDIACRINLTELK
                     LDATSNRYLTFVKGRVGRKIADFFMDFLSAEEGFNPQLQNQTLLQAVSDYCEQGELSA
                     PQTREVKKQVFEYCKGQINSGEEIALNELSNALPTLNEIDFAQFTQEQEYGLEENIPP
                     MRNALKTLTKFSGSGKGVTISFNAELLGERLLWDELNDTLTIKGLPANLRDQLERNK"
     gene            4864..5205
                     /gene="bamE"
                     /locus_tag="PXD71_001898"
     CDS             4864..5205
                     /gene="bamE"
                     /locus_tag="PXD71_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDL6291986.1"
                     /translation="MKMKSLLAAMLLALGVTACSTVEKVVYRIDVPQGNYLEQDKIAQ
                     LKVGMTAEQVEYLLGTPMLKNIFETHRWDYVFIKREGYKDPEQRNLFIYFDAKGLVSK
                     IDDSNANAEQK"
     gene            5398..6738
                     /gene="gdhA"
                     /locus_tag="PXD71_001899"
     CDS             5398..6738
                     /gene="gdhA"
                     /locus_tag="PXD71_001899"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL6291987.1"
                     /translation="MSDLNALFQKIKQRDPNQAPFHQAVEEVFGSLAPFLAKNPKYTQ
                     QGLLGRIVEPERVISFRVTWVDDKGQVQVNRGYRVQMNSAIGPYKGGIRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADTD
                     VPAGDIGVGGREIGYMFGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFAES
                     MLATKGQQIEGKSVVISGSGNVAQYAAEKVIQKGGKVLTVSDSNGYVLFPESGMTEAQ
                     LQALLELKNERRERLSVYATEQGLKYFDGQKPWGVKCDIALPCATQNELDTEDAKELV
                     KNGCICVAEGANMPTTLGGVEVFIGAKILYAPGKAANAGGVATSGLEMSQNAIRLSWS
                     REEVDQRLFNIMKAIHENCVENGTEADGFVNYVNGANIAGFKKVANAMLEQGIV"
     gene            6900..>7096
                     /locus_tag="PXD71_001900"
     CDS             6900..>7096
                     /locus_tag="PXD71_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6291988.1"
                     /translation="MIITMKDIAKEANVSLGTVSNVLNGKSNVSLAKIEKVQAAVEKL
                     GYRKNIQASTLKSGTSNKIAI"
BASE COUNT         2006 a         1287 c         1535 g         2268 t
ORIGIN      
        1 ttcttgctct gcgtagcatt tattttctaa atcaacaatc aacgcttgat aatattctat
       61 gcgtgcttgt aaaccactat ttttattttt gatttcaaca tattcatctg ctgaaagcgt
      121 gccgatattg gctaaattcg ttttttgccg tgcgacaaat tccgcttttt cgttttgcaa
      181 tgcctcaaga atatttttcg ctttgtctaa ttcggtctga atagctaaaa cggctttttc
      241 ttgttcgtgg aatttagctt tttgttggtt aaatgtttca agtgtcataa tttgccctta
      301 ttggttgcgg tgttgttcaa ttttgcgttg aatccattgc tggatttcac tttctagcca
      361 tactttgcta tttgccccaa tactgatact ttggggaaaa tcgccggctt tcattttgcg
      421 ataaatctgc gtttctgaaa gcgtagttac ggctgaaact tcatctattt tcataaatcg
      481 ctgttttgct ggtgtgttct ccattttgtt ttactccgta aaaatttgct ttggcgtgct
      541 attgcctgct aacgtgtgaa agaataaatc atcttggatt ttttgctatt tgttagggct
      601 gtgaaaggag attttacaga ttagaggggc ttaattctga tttggaaaac ttgcaagcgg
      661 ttatttttct ctgatttttt gcaagttttc agcggttctt tcatcgttct gtcatcgagt
      721 ttttaaaagt ggtgtttctg cctgcgtaaa tttgcgtttt cttgcgtaaa attgcgtaat
      781 aaaaaacggc tatatgaaag tttttcacgc tatacggggc aatgtttggg cttaattttg
      841 catttgcgta gaaatcgaga ctttaagtgt gcgggcgagg cgtgggatag acggcacggg
      901 ctgggagctg ataggaaaaa atgaaaattt ttcatcaagc aaaaagaaag gcggattgct
      961 ccgcctttgt catttaaatg ctcttgcatt gttctacaaa atccgcccat tcctgcatta
     1021 ttccacgtct gcggtctagc cagtttttac ggtggtatgc actcattacg gtgtctttgt
     1081 tcgtgtggga taaacaagcc tcaattacta gcgtttcagt agtaaaacgt tcgtgtaaat
     1141 aggtgctggc aatgcttctt aaaccgtgag ccactaaaag ccctttatat tttccaccgc
     1201 ttaagcgttt tattgcatta ttcacggttt ggctattagc cggtttgttt cggtctgttc
     1261 ggtgtgtaaa aatatgttta tggtgtccgt tatatttctt catttcctct aggattgcga
     1321 tagcttgttt tgttagcggt acagaatgcg gattttttcc gcctttcatt ctatctgccg
     1381 gaatgttgag cgtcttagtt tcccaatcta tatcgctcca ttgcaccgct acggcttcaa
     1441 acggtctaag aatattcaag agttgccatt caactaataa tttaatttga ggcgttctat
     1501 ttgatttctg caagtcttgc ataaattcgg ctaactcttc gggcattatt gctggctggt
     1561 gtgtcgtgct tacgctggaa aacgcctttt taatgacgct gattgggtta aaattaattc
     1621 tcccacacaa taccgcataa tcaagaattt ctattaaatt ccctgctatt ctgtgcaaag
     1681 tgtcgggcat tatccgatct aagtccgtta agcggttgat tgtatcggtt aaggtaaaat
     1741 cttccagcgt atagccttca aaacgtggga ataaatgatt ttctaaccgc tgtaagcatt
     1801 tttccattgt tttaggcttg cattttccct ttgatagctt ccaatcagcc catttatagg
     1861 cgaaagattg aatagattct tgcttttcct tagcttctct ttgcattttg gctaaatgct
     1921 cgaaagggtc taagccttgt aaaacaaggg tatttagttc gtctctcttt aagcgtgctt
     1981 cggctaagct catttgcgga tagctaccga ttctattttt tgtgcttcgc ttgcctgttt
     2041 tggggctgat atagctataa atccatactt tcgctttttt ctgtgcaaga ataaatagcc
     2101 cgttgctgtc tgcgataggc ttcgcatttg gggaatattt gagatttttt agtaatgtgt
     2161 ctgaaagtgg ttttttagtt gcagtcattt cttctaatcc ttgatatata agggtttatc
     2221 gaagtttatg atccctaaat tttcccgttt tcaaaatagg gatcatttta gggatcataa
     2281 acactaagat acaatgcaac taaagaagac aataagagac tataaaagaa tctaagttct
     2341 tgattttata gtaaatttga gacaatgaaa gacaatatgc aacgcttaga tggtcccccc
     2401 agccggactt gaaccagcga ccaagcgatt atgagtcgcc tgctctaacc actgagctat
     2461 ggggggattt gaaaagtggt tggaattata gagaaaaacc gagtagtcgt ctagaggcag
     2521 aagattactc ggttaattat tgaacgagag aaaatagggt tattctgttc gccaaattaa
     2581 ggttgccatt cgcccggttt gtttgtctcg gcggtaggaa aaaaacagtt ctggttggct
     2641 gtaggtgcaa tattcaccgc ctgaaatggc ggtaatgcct aatttattca gacgttgggt
     2701 ggcaatttgg taaagatttc ctaaaaattt accgcttgta gtgctgtctt caataagggc
     2761 ttctttcgct cttgggtcaa aagcacagaa ttggtctgcc acttcactcc cgacttgaaa
     2821 agcattcggt ccgatagccg gccctagcca agctgatatt tcgtgtggcg ggcagttgaa
     2881 tttctctacc gtcgcctcta atatgccatc gcataaccca cgccagccgg cgtgtgccgc
     2941 cgcaatttcc gacccgtctt ggctggcaaa taataccggc aaacaatctg cagtcatcac
     3001 taagcagact tggtttggtt gattggtgta aatcgcatcg gctttaatat tatcacctga
     3061 atagggaagt tcaatcactc gggtgctgtg agtttgggtt aaaaacagcg gagcttgtgg
     3121 taattgaaat ttctcaacca ataaagcacg attttgagcg atatcttgtg gctcgtcatt
     3181 aacgtgatca cctaagttga aactgtcaaa aggcggtttg cttactccgc ctgttcttaa
     3241 ggtcgtcagt gcgtgaatat gtttaggcac attccaattt ggaataattt tatccatttg
     3301 caaaaaatct caagaaaatg accgcttgtt atcggcgtac tgcaatcgct tcgatctcaa
     3361 tgccaacatc ttttggtaaa cgagccactt ctacgcaaga acgagccgga aaattagggt
     3421 gttcgttttc ttggaagaag gcttggtatt ccgcatttac ctttgcgaag tcgttaagat
     3481 ctttgacaaa tacggtggtc tttacaatat cggcaacggt taagccggct tgttcgataa
     3541 tggctttcac gttgttcaga gattggcggg tttgttgcac aatatcactc gggatttcgc
     3601 cggtttcagg attcacggga atttgcccag aagttaaaac catattgcct aaatcaactg
     3661 cttgcacata aggcccaatc gctgccggtg cattttctgt gtggataact gtcatcttgt
     3721 gttcctctta atgaaaaata aacggattaa tgataatatc ttttcttatt atttggaata
     3781 aagagaagtg aattaatgag cattaatgtt tctgaaatcg tgttacatca attacaaaaa
     3841 acagagggcg agaacccgga gcttaatacg tttttgcgag aaaatttatt ggcaatttcc
     3901 cctgaagtcg atcaaatgat gttgcaatta catcaagcct atcaaagtaa agcaaaggct
     3961 tacggtatat ttaaaccgga gtcggttttc gctcaacagc tcaaccgctt gttggagcaa
     4021 gaaaccgatt ttttaccttt tagccatagc tgtgccaaaa tgcttgcagc agagctgatt
     4081 aaatatccgt ttgccgatgg tggcactttt attttgtgtc gttacacttt tttagctacc
     4141 gaatatttgt ttattgcgtt gattgatagc cgtaattcaa tgctggttga tgagcagtta
     4201 gagattaaac gcacgcaata tctcgaaatt gcacaatatg atattgcctg ccgcattaat
     4261 ttaactgaac tgaaacttga cgcaacgtca aatcgctatt taacttttgt gaaaggcaga
     4321 gtagggcgta aaattgccga tttctttatg gattttttaa gtgcggaaga agggtttaat
     4381 ccacaattgc aaaatcaaac cttgttgcaa gcggtgagcg attattgtga gcaaggggag
     4441 ttatctgccc ctcaaactcg agaagtgaaa aagcaggtgt ttgaatattg taaagggcag
     4501 ataaatagtg gagaggaaat tgcgttaaat gagctttcca acgcattgcc aacactgaat
     4561 gaaatcgatt ttgcccaatt tacgcaagag caagaatacg gcttggaaga gaatattccg
     4621 cctatgcgaa atgcccttaa aaccctgacg aaattttccg gttcgggcaa aggagtaacg
     4681 attagcttta acgcagaatt attaggagag cgtttacttt gggatgaact aaacgacaca
     4741 ctgacaatca aaggattacc ggcaaatctg cgtgaccagc tggaacgtaa caaataatgg
     4801 aaaattttta tcagtttagg tattatctcg ttatctgaat tttggattat caaaaggtca
     4861 attatgaaaa tgaaatctct tttagctgca atgttgcttg cactgggtgt aacggcttgt
     4921 tcaaccgttg agaaagtggt gtaccgtatt gatgtaccgc aaggcaatta cttagaacaa
     4981 gataaaattg ctcaacttaa agtcggtatg acagcggaac aagttgaata tttgcttggt
     5041 acaccaatgt taaagaacat ttttgaaacc caccgttggg attatgtgtt tattaaacgt
     5101 gaaggttata aagatcctga acaacgcaat ctgtttattt attttgatgc taaagggtta
     5161 gtatcaaaaa ttgatgacag caatgcgaat gcagaacaaa aataggacga tttgttggtt
     5221 cgaaaagccc acttttaaaa gtgggcttat ttttttgaat aaaaaataag catttaaact
     5281 taatttttct tattttaatg ataaattttt ctaatctatc ttttcaaagc taaaattgct
     5341 tgctagactt cgctcatctt aaatacacaa catcatcaat ttattcagga gtataatatg
     5401 agcgatttaa acgccctttt ccaaaaaatc aaacaacgtg atccgaacca agcccctttc
     5461 catcaagcgg ttgaagaagt gttcggtagc cttgcccctt tcttagctaa aaatccgaaa
     5521 tatacccagc aaggtttatt agggcgtatt gttgagcctg agcgtgtaat tagcttccgt
     5581 gtcacttggg ttgatgataa aggtcaagtg caagtaaacc gtggttaccg tgtacaaatg
     5641 aactcagcaa tcggtccata caaaggcggt attcgtttcc acccaaccgt tgatttaggc
     5701 gtattaaaat tcttagcctt tgagcaagta ttcaaaaatg cgttaacgac attgccaatg
     5761 ggcggtggta aaggtggttc agactttgac ccgaaaggca aatctgatgc cgaagtaatg
     5821 cgtttctgcc aagcgtttat gagcgaatta tatcgccaca ttggtgctga tactgacgtt
     5881 cctgcaggcg atatcggcgt gggaggtcgt gaaatcggct atatgttcgg tcaatacaaa
     5941 aaattacgca atgaatttac ttcagtatta accggtaaat cgctgacttg gggtggtagc
     6001 ttaattcgtc cggaagcaac tggttatggt gcggtgtatt ttgcagagtc tatgttagcg
     6061 actaaaggtc agcagatcga aggaaaatca gtggtaattt ccggttcggg taacgtggca
     6121 caatatgcag ctgaaaaagt gattcaaaaa ggcggtaaag tattaacggt ttcagattct
     6181 aacggttatg tcttattccc tgaatccggt atgacagagg cacaattaca agcattatta
     6241 gagttgaaaa acgaacgccg tgaacgctta tctgtgtatg caacagaaca aggcttaaaa
     6301 tactttgacg gtcaaaaacc gtggggtgtg aaatgtgaca tcgctttacc ttgtgcaacc
     6361 caaaacgaat tagataccga agatgctaaa gaattagtga aaaacggctg tatttgtgtg
     6421 gcagaaggtg caaatatgcc aaccacatta ggtggtgttg aagtctttat cggtgcgaaa
     6481 atcctttatg ctccgggtaa agcggctaac gcaggtggtg tggcaacctc aggtttagaa
     6541 atgagccaaa atgcaattcg tctatcttgg agtcgtgaag aagtagatca gcgtttattc
     6601 aacattatga aagcaattca cgaaaactgc gtagaaaatg gtactgaagc agatggtttc
     6661 gtaaactatg taaacggtgc gaatattgct ggtttcaaaa aagtggcaaa tgcgatgtta
     6721 gaacagggta ttgtttaatt tattatccct attattcaag aactgcctcg atatatcgag
     6781 gcagtttttt tattttaggt tgtagctaga gacaagctaa attgaaatcg tatttcattt
     6841 gccaattatg gtaaaatcta tgccaatctc agttttagca gtatgtgaaa gggcagtata
     6901 tgatcattac gatgaaagat atcgcaaagg aagcaaatgt ttcgttagga acagtatcga
     6961 atgtattaaa cggaaaaagt aatgttagct tagctaaaat agaaaaagta caagctgccg
     7021 ttgaaaagtt gggttatcgt aaaaatattc aagcctctac gttaaaaagt ggcacaagta
     7081 ataaaattgc tattat
//
LOCUS       DANLXS010000125         7095 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.125, whole genome shotgun sequence.
ACCESSION   DANLXS010000125 DANLXS010000000
VERSION     DANLXS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.125"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..747)
                     /locus_tag="PXD71_001901"
     CDS             complement(<1..747)
                     /locus_tag="PXD71_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL6291989.1"
                     /translation="MITVKNLHHKIGNATILNNINLTIPNGGITALIGANGAGKSTLL
                     SLIARLQPIQQGEIHLNELNIANTASQTIARQLAILTQDNVIHSRITVKDLLLFGRYP
                     HHQGRITSHDNAIVEEALVRFDLSTFSHRFLNELSGGQRQRALIAMTFCQQTDHILLD
                     EPLNNLDMFHARELMKLLRKLTDELALTTIMVVHDINIAAAYADTIIAMKDGGIKMIG
                     TPKEIITTENLKTVFNLDAEVLECQGKPLVI"
     gene            complement(767..1643)
                     /locus_tag="PXD71_001902"
                     /pseudo
     CDS             complement(767..1643)
                     /locus_tag="PXD71_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
     gene            complement(1696..2664)
                     /locus_tag="PXD71_001903"
     CDS             complement(1696..2664)
                     /locus_tag="PXD71_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL6291990.1"
                     /translation="MFSFFRLNLFLLILLIPISASVGVADFHWSEIFHSSEQSQIFFI
                     SRLPRTLAVILVGATLAVAGMVLQIVLKNRFIEPSMIGTSQSAAIGILLASLLFPTSA
                     LLLKMSIATLSALMGMGLFMLLIRQLPPHQQLMLPLIGIVFGNVIEAISTFIAYETDS
                     LQVLSIWFSGDFSGILAGRYELLWITAILAVVVYVMADQLTIAGLGENISTNLGINYK
                     QMTWLALIVVAMITAIVVVTVGQIPFIGLVVPNIVSRIAGDRLRKNLPAVVVLGANLV
                     LICDILGRAINAPYEVPISTIFGIIGTVIFLYLLLKEKRAEEKARG"
     gene            complement(2758..3657)
                     /locus_tag="PXD71_001904"
     CDS             complement(2758..3657)
                     /locus_tag="PXD71_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6291991.1"
                     /translation="MIKKALFALTATAVFAASAFAKDVTIPTARGEVTFKETPSKIAV
                     FDTGSLDTLQALGVKIDGMPEFKVLSYLKPSADKAVNVGTVFEPDLEALNSLNPDLII
                     VGTRTAKKFDEVSQIAKTIDMTDNGDKLIESGLQRLDSFGKLFNKEAETEKLKAEINT
                     LLDQTKAAVKGKGNGLIILVNGGKISAFGSGYRLSWIHDNLGIPMADSSIKNTGHGQP
                     VSFEFIQKLNPDWLFVLDRIAAIGQEGQSAQQVLDNELVRQTKAWKNGNIVYLSAASY
                     LAPGGYEQMKTDLTNIKTAFEKK"
     gene            3831..5294
                     /locus_tag="PXD71_001905"
     CDS             3831..5294
                     /locus_tag="PXD71_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL6291992.1"
                     /translation="MAEYSGTTQEKMKDKQVEQINISPHSIEAEQAVLGGIMLSNDHW
                     DNVAERVQPHDFYNYAHRIIFEQMAELVRHNKPIDIITLDQALKDKGVLQDVGGFAYL
                     AELSKNTPSAANILAYADIVRDRSDLRSVISAGNQIAEMGYKTKGRNSKEVLDEAERI
                     VFEIAEKRNNANEGPQRIDDILLKTLARMDTLSKNRHNGGVTGVSTGFVDLNKKTAGL
                     QPSDLIIVAARPSMGKTTFAMNLCENASLVDIEDKDDLDEQGNPKKKPAKPVLIFSLE
                     MPADQIMMRMLASLSRVDQTKIRTGQISDDEDTAKISSTMAILQERNNIYIDDSSGLT
                     PTELRSRARRIYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEV
                     PVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAE
                     IIIGKQRNGPIGRVRLTFQGQYSRFDNYTGGHYDDEY"
     gene            5284..5589
                     /locus_tag="PXD71_001906"
     CDS             5284..5589
                     /locus_tag="PXD71_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDL6291993.1"
                     /translation="MNTKPTQPITLCAIYRSKAKEGMYLYVPKRDQFEQVPETLRQLF
                     GKPEFVMMFNLTGEKPLVRAKNEEVLQLLIEQGYYLQMPPPPENLHKAFIAQQKGEK"
     gene            5589..6038
                     /gene="nrdR"
                     /locus_tag="PXD71_001907"
     CDS             5589..6038
                     /gene="nrdR"
                     /locus_tag="PXD71_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDL6291994.1"
                     /translation="MRCPFCSTEETKVIDSRLAASGYQIRRRRECISCKERFTTFESA
                     ELVVPYVIKNNGSREPFDAEKLRTGLRRALNKRPVSADDVEKAISQIIIQLQAMGERE
                     VPSRHIGTLAMNALKQLDKVAYIRFASIYLSFSDIEEFIKEIEKLKE"
     gene            complement(6112..6294)
                     /locus_tag="PXD71_001908"
     CDS             complement(6112..6294)
                     /locus_tag="PXD71_001908"
                     /inference="COORDINATES: protein motif:HMM:NF015431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6291995.1"
                     /translation="MALLADSTVDRLVAENGLVEVLGETENPKFDLTMIYAAHRYPSS
                     IVRAFVGFVLEQVKAN"
     gene            complement(6263..6973)
                     /locus_tag="PXD71_001909"
     CDS             complement(6263..6973)
                     /locus_tag="PXD71_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6291996.1"
                     /translation="MNLNTLRLFVAVIQHGSLSKASERLNVPIATISRQIADLEKELN
                     IQLFDRQKSGVKPTMAGQRLYEQVHLSIEQLATAKEVLFEEQGNLKGKLRLSASPACE
                     PVLAWIADFQAAYPQVQVHCTLTDRLLDLSADGIDVAFRIGQLHGEQFIAKKVGQIGS
                     KWVAHPKFLARFGTPSTVKDLKNFPIAAWAKNEASVITLGTGRQAVEIPYLFSSNDSY
                     AIEYMAKSSGSACRQHGG"
BASE COUNT         2243 a         1427 c         1457 g         1968 t
ORIGIN      
        1 aataactaaa ggtttgcctt ggcattctaa cacttcggca tcaagattaa acactgtttt
       61 taaattttca gtggtgataa tctcttttgg ggtgccgatc atcttaattc caccatcttt
      121 cattgcaata atagtatctg cgtaagcagc agcaatgttg atgtcgtgta ccaccattat
      181 agtagttaag gcgagttcat cggttaattt tcgtaataat ttcattaatt cccgagcatg
      241 gaacatatct aaattattca gtggttcatc taataagata tgatcggttt gctgacagaa
      301 ggtcattgca attaatgccc gctggcgttg ccctccggat aattcattta agaatcggtg
      361 ggagaaagtc gataaatcaa atcgtaccag agcctcttct acaatggcat tatcgtgcga
      421 agtgattctg ccttgatgat gagggtaacg cccaaataac agcagatctt tgacggtaat
      481 acggctatga atcacattat cttgggtcaa gatagccagt tgtcgagcga tagtttgaga
      541 agctgtgttg gctatattca gctcatttaa gtggatttca ccctgttgga taggttgcaa
      601 gcgtgcaatt aaagagagca gggtggattt gcccgcacca tttgctccga ttaaagcggt
      661 aataccacca tttggaatgg ttaaattgat attattcaga atcgtggcat tcccgatttt
      721 gtggtgaaga tttttaaccg ttatcatttt cttccttaat ttatggttat tttttccgtt
      781 ttaaaatgag ataaataaag acaataccgc caataaattc aataacgaca ctcagaaccc
      841 cttgcatttt cagcacttgc tcgaagattg cctgccctaa aattaaggtg atggcagaga
      901 ttaaaaaagt cattggtatt cgcacgctat ggcaaacagt cgggctaatt gcattaacaa
      961 tggcacaaac aagtagccct agaaatagaa tcgggccaac aagtgcggtt gaaacagaca
     1021 ccaacaatcc acaacagatt agtaattgac gtgaaaattg atgatattct aacccaagcc
     1081 caattgcctt atctttgcca agtaacaaaa tatctaattt atgccgttgt aaccagaccc
     1141 aaatggcaga gagaatagcg atacagcctc caatccaaag taatgtgggg gtagtcaggt
     1201 taaatgaggc aaagaagagc cttgagcaac ggcaaactct gttgggtcga cattcgttgc
     1261 aacaaattat ttaggcttct aaataagaca ccaaagatga caccgattaa aagcattcgg
     1321 gttaaatcgg atcgatttcg attcagtgaa ctaaatagta atattgatgc ccctagcatt
     1381 aacaaggttt caaagaaaaa cttatagctt gggtctaact gagtaaagcc taaaccgcca
     1441 aataaaaata ctaacgaggt ttgcaatagc aagtagagcg aatcgaaccc caaaatactt
     1501 ggagttaaaa taggattatg tgtgagtgtt tggaatagca gggtcgaaat accaatcgta
     1561 taagcaatag tacagagcaa aataagcttt ttgccacgca gttgcaatat aaagtcccaa
     1621 ttacctttcg cattataggt catataaaac agaatacttg ctaataagat taagcttaaa
     1681 acgataaagc gtgatctaac cacgagcctt ctcctcagcc cgtttctctt tgagcaataa
     1741 atagaggaaa atcactgtcc caataatccc aaaaatagta gaaatcggca cttcataagg
     1801 ggcattgatg gctcgcccta aaatgtcaca aatcaggact aaattcgcac ctaacaccac
     1861 gaccgcaggc agatttttac gcaatcggtc acctgctatg cgagagacaa tattagggac
     1921 gaccaagccg ataaacggaa tctgtccaac cgtcaccacg acaatagcgg taatcatcgc
     1981 caccacaatt aaggcaagcc aagtcatttg tttgtagtta atgcctaagt tggtactaat
     2041 attttcacca aggcctgcaa tggttaattg atctgccatc acataaacca caacagccag
     2101 aatagcggta atccagagca gttcataccg acctgccaaa ataccggaga agtcgccgga
     2161 aaaccaaatc gaaagcactt gtaggctgtc agtttcataa gcaataaaag tgctgatagc
     2221 ttcaataaca ttgccaaaaa cgatcccgat taatgggagc attaattgtt gatgaggcgg
     2281 taattgacga atgagcaaca taaaaagccc cattcccatt aatgcactta atgtggcaat
     2341 agacattttt aacagtaatg ctgatgttgg gaaaagtaaa ctagcaagta aaataccaat
     2401 tgccgcactt tggctagtgc caatcatact aggctcaata aagcggttct tcagcacaat
     2461 ttgtaatacc atacctgcaa cggctaaggt tgcaccgact aatatcactg cgagcgttct
     2521 tggtaaacgg ctgataaaaa agatctgact ttgttcggaa gaatgaaaaa tttcactcca
     2581 atgaaaatct gccacaccca cactggcact gattggaatg agcaatatta aaagaaatag
     2641 atttaatcta aaaaaagaga acatagtgat aaatagcaag cggtcatatt ctctctcttt
     2701 tttacaaatg caggcacttg caaaaatagg aggaaatctg accgcttgta aggttaatta
     2761 ttttttctca aatgctgttt taatgttagt taggtcggtt ttcatctgct cataaccacc
     2821 cggcgctaaa tatgaggcag cacttagata aacgatatta ccgtttttcc aagctttagt
     2881 ttgacgaact aattcattat ccaacacttg ttgggctgat tgaccttctt gtccgatagc
     2941 cgcaatacgg tctaacacaa acaaccaatc cggattgagt ttttggataa attcaaacga
     3001 aacaggctga ccgtgaccgg tatttttaat gctagaatct gccattggaa tccctaaatt
     3061 atcgtgaatc caacttaaac gataacctga accaaatgca gaaattttgc caccattgac
     3121 taaaataatc agtccgttac ctttaccttt tactgctgct ttggtttggt ctaataaagt
     3181 attaatttct gcttttagtt tttcggtttc cgcttcttta ttaaataatt tgccgaaaga
     3241 atcaagacgc tgtaatccgc tttcgattaa tttatcgcca ttatccgtca tgtcgatggt
     3301 tttcgcaatt tggctgactt catcaaattt ttttgctgtg cgtgtgccaa caatgatcaa
     3361 atccggattt aaagaattta acgcttctaa atccggctca aataccgtac cgacattcac
     3421 cgctttgtct gcactcggtt taagataaga aagcacctta aattcaggca taccgtcaat
     3481 ttttacacct aaggcttgta aagtatccaa acttccggtg tcaaacacgg caattttaga
     3541 cggggtttct ttgaaagtaa cctctccacg tgcagttgga atagtaacat ctttggcaaa
     3601 tgcagaagca gcaaacacag cagttgcggt caatgcaaac aaggcttttt taatcattgg
     3661 ataactccat ttatattgaa aataattctc aaagatttta acgaaaattg attttaagta
     3721 aagcggaaaa taacaagcgg gcgaattttg cctaaaattt gcaaatttca atttaattaa
     3781 aattccgata cactatgtac atcaatacgt cattatagca agagaaaaat atggcagaat
     3841 atagcggaac aacccaagag aagatgaaag ataagcaggt tgagcaaatt aatatctcac
     3901 cacattcgat tgaggcagag caagcggtgc ttggtggcat tatgctcagt aatgatcact
     3961 gggataatgt tgctgagcgt gtacaaccgc acgattttta caactatgcg catcgaatta
     4021 tttttgaaca aatggcggag ttggtgcgcc acaataaacc gattgatatt attacgctgg
     4081 atcaagcctt aaaagacaaa ggggtgctgc aagatgtagg aggttttgcc tatttagccg
     4141 agctttctaa aaataccccg agtgcagcaa atattttagc ctatgcggat attgtgcgag
     4201 atcgttccga tttaagaagt gtaatcagtg ccggcaatca aattgcggaa atgggctaca
     4261 aaacgaaagg gcgtaactcc aaagaagtgt tagacgaggc agagcgaatt gtgtttgaaa
     4321 ttgcagaaaa acgcaataac gccaatgaag gtccacaacg tattgacgat attttgctta
     4381 aaacactcgc tcgaatggat acgctatcta aaaatcgcca taacggcgga gttactggtg
     4441 tttctaccgg gtttgttgat ctcaataaaa aaaccgcagg gttacagcct tcggatttaa
     4501 tcattgtggc agcccgtcct tcaatgggga aaactacttt tgcaatgaac ttatgtgaaa
     4561 atgcctcatt agtggatatt gaagataaag atgatttaga cgaacagggt aatccgaaga
     4621 aaaaaccggc aaagccagtg ctaattttca gcctggagat gccggcagac caaattatga
     4681 tgcgtatgct tgcctcactt tctcgggtcg atcaaaccaa gatccgaacc ggtcagattt
     4741 cagatgatga agacactgct aaaatctcca gcacaatggc aattttacaa gaacgtaata
     4801 atatctatat tgatgatagc tccggtttaa caccgaccga attacgttct cgagcgagac
     4861 ggatttatcg tgaaaatggc ggattaagct tgattatggt ggactatctc caattaatgc
     4921 gagcgccggg atttgcagat aatcgtactt tagaaatcgc agaaatttcc cgttctttaa
     4981 aggcactagc gaaagaatta gaagtgccag tagtcgctct ttctcagcta aaccggagtt
     5041 tggaacaacg tgccgataaa cgtccggtaa actcggattt gcgtgagtca ggttctattg
     5101 agcaagatgc agacttaatt atgtttattt atcgtgatga ggtctataac gacaattccg
     5161 atctgaaagg cattgcggaa attattatcg gaaaacaacg taacggcccg attggtcggg
     5221 tgcgtttaac gttccaaggg cagtattccc gttttgataa ttacacagga gggcattatg
     5281 atgatgaata ctaaaccaac ccagccaatc acactttgtg cgatttatcg cagtaaggca
     5341 aaagaaggaa tgtatttata tgtgcctaag cgtgaccaat ttgagcaagt gccggaaaca
     5401 ttacgccagt tatttggtaa gcctgaattt gtgatgatgt ttaatttaac cggtgaaaag
     5461 ccattggtgc gtgctaaaaa cgaagaagtg ttacaattac ttatagagca aggctactat
     5521 ttgcaaatgc cacctccgcc ggagaatttg cataaagcct ttatcgcaca acagaaaggg
     5581 gaaaaataat gcgctgccca ttttgttcca ctgaagaaac taaagtgatc gattcacggc
     5641 ttgcagcaag cggttatcaa attcgccgcc gccgtgaatg tataagctgt aaagaacgtt
     5701 ttactacctt tgaatcggcc gaattagttg tgccttatgt gatcaaaaat aatggtagcc
     5761 gagagccgtt tgatgccgaa aaattaagaa cagggttaag acgtgcatta aataaacgcc
     5821 ctgtcagtgc tgatgatgtc gaaaaagcaa ttagccaaat cattattcag ctgcaagcga
     5881 tgggcgaacg agaagtgccg agtcgccata tcggtacatt ggcaatgaac gcacttaaac
     5941 aattagacaa agtagcatat atccgctttg cctctattta tttaagcttt agtgatattg
     6001 aagagttcat taaagaaatt gaaaaattaa aagagtagtt gcaaaaaaac ataaaaaaat
     6061 gaccgcttgt gagtttaaaa tccacaagcg gtcatttttc tttaaaaata ctcaattagc
     6121 ttttacttgt tccaacacaa accctacaaa cgcccgcaca atgctagacg gataacgatg
     6181 ggcggcataa atcatcgtca aatcaaattt ggggttttct gtctcaccca agacttccac
     6241 caagccattt tctgccacca atctatccac cgtgctgtcg gcaagcagag ccactgcttt
     6301 ttgccatata ttcaatggca tagctgtcat tgctggaaaa aagataggga atttctaccg
     6361 cttgtctgcc agtgcctagc gtgatgacgc ttgcttcatt ttttgcccaa gcggcaatgg
     6421 ggaaattttt caaatctttg acggtgctgg gcgtgccaaa gcgtgccaaa aatttagggt
     6481 gtgccaccca tttactgccg atttgcccca cttttttggc gataaactgt tcgccgtgta
     6541 gttgcccaat ccgaaacgcc acatcaatgc catctgccga caaatccaaa agacggtcgg
     6601 tcagcgtgca atgcacttgt acttgtggat aagctgcctg aaaatcagct atccacgcca
     6661 aaacaggctc gcaggcaggg cttgccgaca ggcgtaattt accctttaaa ttgccttgct
     6721 cttcaaacaa cacttctttg gcagtggcaa gctgttcaat ggacaaatgc acctgttcat
     6781 acaggcgttg ccctgccatt gtgggtttaa cgcctgattt ttggcggtca aagagttgaa
     6841 tgttaagctc tttttccaaa tcggcaattt gacggctaat ggttgcaatc ggcacattga
     6901 gtcgctcgga ggctttggag agactgccgt gctggataac ggcgacaaag aggcggaggg
     6961 tgttgaggtt cattgttttt ctccccaatg ttcaatttct tgaataaaat gtttaatact
     7021 gccttggact tgttgatttt taccatatag ataataaacg cctgaaagtt cttgtaaaaa
     7081 tttactttct ttata
//
LOCUS       DANLXS010000126         7066 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.126, whole genome shotgun sequence.
ACCESSION   DANLXS010000126 DANLXS010000000
VERSION     DANLXS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.126"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(29..814)
                     /gene="znuB"
                     /locus_tag="PXD71_001910"
     CDS             complement(29..814)
                     /gene="znuB"
                     /locus_tag="PXD71_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDL6291997.1"
                     /translation="MFDILFPAWLAGVLLAFITAPLGAFVVWRKMAYFGDTLSHSALL
                     GVALGIFLELDPYLAVIGMTILLALLLVWLEQKTNYSVDTVLGIIAHTSLSLGVITIS
                     LLDNVRVDLMSYLFGDLLAINFSDVVFIGIGATLICLILMLFWKRLLSITISPELAQI
                     EGLNVARLRLLLMILTALTIALSMKFVGALIITSLLIIPSATARRFAKTPEKMVLIAI
                     FFSIIAVSGGLLLSSWKDTPAGPSVVICAGLIFLLSLFKKEYH"
     gene            complement(901..2238)
                     /locus_tag="PXD71_001911"
     CDS             complement(901..2238)
                     /locus_tag="PXD71_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL6291998.1"
                     /translation="MSSLSFDFSEDFRPLAAKMRPRNLAEYVGQSHLIGEGKPLRRAI
                     EAGHSHSMIFWGPPGTGKTTLAEIIAHHLDAEVERISAVTSGVKEIREVIEQAKLNRQ
                     AGRRTLLFVDEVHRFNKSQQDAFLPHIEDGTIIFIGATTENPSFELNNALLSRARIYI
                     LKPLQAVEILKILQMAIADTERGLGNETLVLEDDVLALLADYVNGDARFALNCLELMV
                     DMAQDSAKGKVLNKSLLTEVLGERQARFDKGGDRYYDLISALHKSIRGSSADGALYWY
                     ARILTAGGDPLYVARRLLAIASEDIGNADPRAMQIAIVAWDCFTRVGAYEGERAIAQA
                     IIYLAVAPKSNAVYQAFNEAKRLAKEAKDYDVPAHLRNAPTQLMKSLGYGEEYRYAHN
                     EPNAYAAGENYFPPELKDTEFYFPSERGMEKQIKEKMAWLKAQDQASPIQRYK"
     gene            complement(2248..2736)
                     /gene="mreD"
                     /locus_tag="PXD71_001912"
     CDS             complement(2248..2736)
                     /gene="mreD"
                     /locus_tag="PXD71_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL6291999.1"
                     /translation="MRTNPIFQILVIIAIFVVAFVLEIMPWPVGFQGLRPTWVVLVLI
                     YWALALPDKMSVGTAFIAGIVWDLILGSILGIHALVLSVAIYFVAKYHLILRNLSLWL
                     QSLLVMAYIVLIRFFIFVIEYILHSAEFNSQELLGAVISGVLWSWIFLLMRHIRRQLR
                     LR"
     gene            complement(2736..3791)
                     /gene="mreC"
                     /locus_tag="PXD71_001913"
     CDS             complement(2736..3791)
                     /gene="mreC"
                     /locus_tag="PXD71_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL6292000.1"
                     /translation="MKPIFAKAPSLGMRLIIAVIFSIMLIISDGRSSLMIQARNLLET
                     AVSGLYYFANTPRSILDGVSGNFIDNNKLQLENNLLKEQLREKSADLLLLDQLKVENQ
                     RLRLLLSSPLRQDEYKKIAEVLAAEMDAYRQQVVINQGRLDGAFVGQPIIDERGVVGQ
                     VISVGEKSSRVLLLSDITHAIPVQVLRNDVRGIASGTGVNNELVIDNLPRSIDVVKGD
                     VLVTSGLGGRFPEGYPVAIVETVENDTKSQFARIVARPLASFDRLRYLLLLWPTAEEK
                     NYSQSLSPAQVREVVEERRNSVNPFERLKQMSNKKTEVQLEAKEEQIENDEINENPAA
                     QPEAENEQNYHTEEGAE"
     gene            complement(3893..4945)
                     /locus_tag="PXD71_001914"
     CDS             complement(3893..4945)
                     /locus_tag="PXD71_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDL6292001.1"
                     /translation="MFKKFLGLFSNDLSIDLGTANTLVYVKGQGIVLDEPSVVAVRQD
                     RMGSLKSIAAVGSHAKLMLGRTPKSIMAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPAGATQVERRAIKESAIGAGAREVYLIEEPMAAAIGAGLPVHEAMG
                     SMVIDIGGGTTEVAVLSLNGIVYSTSVRIGGDKLDEAIIAYVRRHFGSAIGEATAERI
                     KQEIASAKIESDDDVKTMEVHGHNLAEGAPRTFELNSRHVLEAIEQPLNGIVEAVKSV
                     LEQCPPELAADIFERGMVLTGGGALLHNLDRLLSDSTGVQVVVAEDPLTCVARGGGKA
                     LEMIDMHGGDVFSIDD"
     gene            complement(5060..5689)
                     /locus_tag="PXD71_001915"
     CDS             complement(5060..5689)
                     /locus_tag="PXD71_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDL6292002.1"
                     /translation="MDIFIQGFIVCFGLIVSIGAQNAFLLKQGILKQHIFWIALLCFL
                     GDVFLMTIGVLGLGTLIAELPVVSFIISLLGAIFLLSYGSRSFISVFKSGEYLVASGE
                     NATSLKKALLITFAITFLNPHVYIDTVVIVGSIGGKLDFNGKIYFLAGALLCSFIWFF
                     GIGYGASLLSPYFAKRRTWQILDAVTGLIMYFIAFSLLIYTYQLALQIF"
     gene            5793..6440
                     /gene="udk"
                     /locus_tag="PXD71_001916"
     CDS             5793..6440
                     /gene="udk"
                     /locus_tag="PXD71_001916"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL6292003.1"
                     /translation="MTDTSQTSSCVVIAIAGASASGKSLIASTIYKELKNELGTDNIG
                     IISEDAYYRDQSHLTMEERIKTNYDHPNSMDHHLLVEHLRQLKQGNAIEIPEYDYTEH
                     NRKTTTKHFEPKKIIILEGILLLTDENIRNEINVSIFVDAPLDICFIRRLQRDMVERG
                     RSMESVISQYRKTVRPMFLQFIEPSKQYADIIVPKGGKNRIAIDILKAQIKQLLK"
     gene            6455..7036
                     /gene="dcd"
                     /locus_tag="PXD71_001917"
     CDS             6455..7036
                     /gene="dcd"
                     /locus_tag="PXD71_001917"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDL6292004.1"
                     /translation="MRLCDTDIKRYLDEGIIAITPRPSDDKISGATADVRLGNSFRVF
                     REHKTPYIDLSGPREEVAAQLNKVMSDEIIIGDDEAFFLHPGDLALATTLESVSLPAN
                     IVGWLDGRSSLARLGLMVHVTAHRIDPGWEGKIVLEFFNAGKLPLALRPNMAIGALSF
                     EILSGDAAKPYNARKDAKYKNQQSAISSRINQD"
BASE COUNT         2275 a         1579 c         1234 g         1978 t
ORIGIN      
        1 tatgctttat tttcatacat tatgctgttt aatggtattc ttttttgaat agcgataata
       61 gaaaaatcaa gccggcacaa atgactaccg aaggccctgc tggtgtatct ttccaactgg
      121 ataacaataa accgccactg actgcaataa tactgaagaa aatcgcaatc aacaccattt
      181 tttcaggcgt tttagcaaag cgtcttgccg tagcagacgg aataatcagc agtgaggtaa
      241 taattaaagc accgacaaat ttcatactta atgcgatagt taatgcggtt aaaatcatca
      301 gcaataaacg taagcgagcc acatttaatc cttcaatttg agcaagttcc gggctgatgg
      361 taatcgacaa caaccgcttc caaaacagca ttaaaatcaa acaaattagg gttgctccga
      421 ttccgataaa caccacatca gaaaaattaa ttgccagtaa atcgccgaat aagtaagaca
      481 tcaaatccac tcgtacatta tccaacaaac taatggtaat taccccgaga gaaaggctag
      541 tatgggcgat aatccccaac acggtatcca ccgaataatt ggttttttgc tccaaccaaa
      601 ccagcaataa tgcaagaaga atagtcattc caattacagc aagatacgga tcgagttcta
      661 aaaaaatccc taatgccacg ccgagcaatg cagagtgcga aagtgtgtcg ccgaaatacg
      721 ccatttttcg ccacaccaca aaagccccta gaggtgcggt aataaaggct aacaaaacac
      781 cggcaagcca agccggaaat aaaatatcaa acatattggt tctcaaaggc aagtaaaaca
      841 agcggttatt ttttcggaaa aatttgcaaa ttttttgctg atttagaccg cttgtcatat
      901 ttatttataa cgttgaatcg ggctggcttg atcctgtgct ttcagccacg ccattttttc
      961 tttaatctgt ttttccattc cccgctcact tgggaagtag aattcggtat ctttcagctc
     1021 aggcgggaaa taattctccc cggcggcata agcgtttggc tcattgtggg catagcgata
     1081 ctcttcgcca tagcctaaag atttcattaa ttgagtggga gcattacgca aatgcgccgg
     1141 cacatcgtag tcttttgcct ctttagcgag gcgctttgct tcattaaaag cttgatagac
     1201 cgcattgctc tttggggcaa ctgccaaata aatgatcgct tgggcaatcg ctctttcacc
     1261 ttcataagcc cccactcggg taaagcaatc ccaagccaca atcgcaattt gcattgctcg
     1321 agggtcggca ttaccaatat cttcagaagc aatcgcaagc agccttcttg ccacataaag
     1381 cggatcacca ccggcggtta aaattcgggc ataccagtat aatgctccat cagccgatga
     1441 gcctcgaatg gatttatgta acgcagaaat gagatcatag taacgatcac cgcctttatc
     1501 aaaacgagct tgtcgttcgc ctaacacttc ggtcagcaaa cttttattca gcactttccc
     1561 tttagctgaa tcttgtgcca tatctaccat taattccaaa cagttcaagg caaaacgggc
     1621 atcaccatta acataatcag caagcaatgc cagtacatca tcttcaagta ccaaagtttc
     1681 attgcctaaa ccacgttcgg tatcggctat cgccatttgc aaaattttta gaatttcgac
     1741 cgcttgtaac ggctttaaaa tataaattct ggctcgggaa agcagtgcgt tatttagctc
     1801 aaaagaggga ttttcggtag tcgcaccaat aaaaataata gtgccgtcct caatatgcgg
     1861 taaaaacgca tcttgctggc ttttgttaaa gcggtgaact tcatccacaa aaagcaacgt
     1921 tcgtctgccg gcttggcggt ttaatttggc ttgttcaatc acctctcgga tctcttttac
     1981 accgctggtc acagcggaaa tacgttcaac ttccgcatca agatgatggg cgataatttc
     2041 agctagggtc gtttttccgg tgccgggcgg tccccaaaaa atcatagaat gactatgtcc
     2101 ggcttcaatc gctctgcgta acggtttgcc ttcgccaatt aaatgagatt gcccgacata
     2161 ttcagccaag tttcgaggtc gcattttggc tgcaagcggt cgaaaatctt cactaaaatc
     2221 aaaagacaaa ctactcattt taacctctta acgtaaccgc agttggcgac gaatatgacg
     2281 cattaataag aaaatccaag accaaagcac accactgatt acagcaccta acaattcctg
     2341 tgaattaaat tcggcactgt gtaaaatgta ttcaatcacg aaaataaaga aacggatcag
     2401 cacaatatat gccattacta ataggctttg tagccatagc gataagtttc gcagaatcag
     2461 atggtatttc gcaacaaaat aaatcgccac cgataacact aaggcgtgga tgcctaaaat
     2521 cgagccaaga atcagatccc agacgatgcc ggcaataaaa gccgtcccca cactcatttt
     2581 atcaggtaat gccaatgccc aataaattaa caccaaaacg acccaagtag ggcgtagccc
     2641 ttggaatcct accggccaag gcataatctc aagtacaaaa gcaaccacaa aaatcgcaat
     2701 tatcacaagg atctgaaaaa tcgggtttgt tctcattatt ccgctccttc ttccgtatga
     2761 tagttttgtt cattttccgc ttctggttgt gctgccggat tctcatttat ctcatcattc
     2821 tctatttgtt cttcttttgc ctctagctgc acttcagttt tcttgttaga catctgtttt
     2881 aaacgttcaa atggattcac gctattacgt ctttcttcaa caacttcacg tacttgcgct
     2941 ggcgacaagc tttgggagta gtttttctct tccgccgtcg gccaaagcag taataagtaa
     3001 cgtaagcgat caaatgaggc taacggacga gcaacaattc tggcaaattg acttttggta
     3061 tcattttcaa ccgtttctac aatcgccacc ggatatccct cagggaaacg tccacctaag
     3121 cctgaagtca ccagcacatc accttttacc acatcaatag aacgaggcaa gttatcaatg
     3181 accaattcat tattcacccc tgtaccactg gcaatgccac gtacatcatt gcgtagcact
     3241 tgaacaggaa tagcatgagt aatatcagag agcaataaaa cacggctaga tttttcccct
     3301 acagaaataa cttgtccaac aacacctctt tcatcaataa taggttgccc gacaaaagca
     3361 ccgtctaaac gcccttgatt aatcactact tgctgacgat aagcatccat ctcagcagct
     3421 aatacttcag caattttttt atactcatct tgccgaagcg gagaactcaa taacaatctt
     3481 aaacgctgat tttcaacctt tagctgatca agcaataata aatcggcact tttttctctt
     3541 agctgctctt ttaataaatt attctcaagc tgtaatttat tattatcaat aaaattgcca
     3601 cttaccccat ctaaaattga acggggggta ttagcaaaat aatataatcc acttaccgcg
     3661 gtttctaata gattacgcgc ttgaatcatt aaagagctac gcccatcgga aataatcagc
     3721 ataatagaaa atatcactgc gataatcagg cgcatcccta aagagggagc tttagcaaaa
     3781 ataggtttca ttagagaaat atatgtgaat ttattgggaa tctataacga aaattgacaa
     3841 tagaatcaat ccattgccaa cctccattta aacagctgct aagagaaatg tattaatcat
     3901 caatactaaa tacatcacca ccgtgcatat cgatcatttc taatgcttta ccaccaccac
     3961 gagctacaca agttaatgga tcttctgcta ctacgacttg cacgccagtt gaatcagaaa
     4021 gtaaacgatc taaattatgt aataatgcac cacctcctgt taacaccata ccgcgctcaa
     4081 aaatgtctgc tgccaattca ggtgggcatt gctctaagac tgatttaact gcctctacaa
     4141 taccatttaa tggttgttca attgcttcta atacatgtct tgagttaagc tcgaaagtac
     4201 gaggagcgcc ttcagctaaa ttgtgtccgt gtacttccat tgttttaaca tcatcatcgg
     4261 attcaatctt agcactagca atttcttgtt taattcgctc tgccgttgct tcaccaatag
     4321 cagaaccaaa atgacgacgt acataagcaa taatagcttc atctaactta tcaccaccaa
     4381 tacgcacaga agttgaatac acaatgccgt ttaaagaaag cactgccact tcggttgtac
     4441 cgccaccaat gtcaattacc attgacccca ttgcttcgtg aaccggcaaa cctgcaccga
     4501 ttgcagccgc cattggctct tcaattaaat agacttcacg cgctcctgct ccaattgcag
     4561 attctttaat tgcacggcgt tccacttgtg ttgcacctgc tggcacgcac actaatacac
     4621 gagggcttgg gcgcataaag ttattactat gtacttgttt aataaagtgt tgcaacattt
     4681 tttctgttac aaaaaaatcc gcaatgacac catctttcat tggacgaatc gccattatgc
     4741 tttttggtgt acggcctagc attaattttg catgtgagcc taccgccgca atacttttta
     4801 gcgatcccat acgatcttga cgtacagcaa ccactgaggg ttcatctagc acaatacctt
     4861 gacctttaac ataaaccagt gtatttgctg tcccgagatc gatagaaaga tcgtttgaaa
     4921 ataatcctaa aaatttttta aacataaaag aatccgttaa aataaaatta ttcaaaaata
     4981 agcacatttt atagtgcgaa cattgctact aatatagcaa aaaagcccgt cattcatcaa
     5041 tgaaaaaatt taaactccac tagaaaattt gcagagcaag ttgataagta taaatcagta
     5101 aactaaaagc gataaagtac ataattagcc cggtaacagc atctaaaatt tgccaagttc
     5161 ttcgctttgc aaaataaggg gaaagtaagc ttgctccata acctatgcca aaaaaccaaa
     5221 taaatgagca aagtaatgca cctgctaaaa agtatatttt accgttaaaa tctaatttcc
     5281 caccgatact gcctacaatc acaaccgtat caatataaac gtgaggattt aagaatgtaa
     5341 tcgcaaaggt aatcagtaaa gcctttttta agctcgttgc attctctcca cttgccacaa
     5401 ggtattcacc acttttaaat acgctgataa atgaacggct accgtagctt aatagaaaaa
     5461 tggcccctaa aagcgaaata atgaaactta caacaggcaa ttccgcaatt aacgtgccta
     5521 agcctagcac acctatcgtc attaaaaata catcacctaa aaaacagagc aatgcaatcc
     5581 aaaatatgtg ttgttttaaa atcccttgct ttaataaaaa ggcattttgt gcgccaatag
     5641 agacaattaa gccaaaacac actataaatc cttgaatgaa aatatccatt ttttctccaa
     5701 aatcgcataa caagcggtca attatacaaa aaattttgca aaacttcggt ataatttatc
     5761 gaatactctt ttgactgaat aggaaatttt ttatgactga tactagccag acttcatctt
     5821 gcgttgtcat tgcgattgcc ggcgcatctg cctcaggtaa gagcctgatt gcctcaacca
     5881 tctataaaga actcaaaaat gaattgggaa cagataatat cggtattatt tccgaagatg
     5941 cttattatag agatcaatct catctaacaa tggaagaacg gattaaaacc aattacgatc
     6001 accctaattc aatggatcat catttattgg ttgaacattt acgccaatta aaacaaggta
     6061 atgcgattga aattccagaa tatgactata ccgaacataa tcgtaaaacg accacgaagc
     6121 actttgaacc aaagaaaatc attattttag aaggtatttt attgctgaca gatgagaata
     6181 ttcgcaatga aattaatgtt tctattttcg tagatgctcc tctggacatc tgctttatcc
     6241 gccgcttaca acgtgatatg gtggaacgtg gacgttcgat ggaatcagtg attagtcaat
     6301 atcgcaaaac cgttcgccca atgttcttac aatttatcga accctctaaa caatatgctg
     6361 atattattgt gccaaaaggt ggcaaaaacc gtattgccat tgatattctt aaagcacaaa
     6421 ttaagcaatt actaaaataa aataggagct aaatatgcgt ttatgtgata cagatattaa
     6481 acgttactta gatgaaggta ttattgccat aacgcctcga ccttcagacg ataaaatttc
     6541 tggcgccact gcagatgtgc gtttaggtaa ttcgtttcgt gtatttcgtg aacataaaac
     6601 gccctatatt gatttaagtg gccctagaga agaagttgcc gctcagttaa ataaagtgat
     6661 gagtgatgaa attatcatcg gtgatgatga agcatttttc cttcatcccg gtgaccttgc
     6721 actcgcaaca acccttgaat cggtttcctt accagccaat atcgttggat ggttagatgg
     6781 tcgttcgtca cttgctagat taggcttaat ggtacacgtt accgcccaca gaatcgatcc
     6841 gggttgggaa ggcaaaattg tattggaatt ttttaatgct ggaaaattac ccctagcttt
     6901 acgccctaat atggcaattg gcgcattaag ctttgagatt ttaagtggtg atgctgcaaa
     6961 accctataat gcgcgaaaag atgcaaaata taagaaccag caaagtgcca tttcaagtcg
     7021 gattaatcaa gattaatata gtacatgcct tgcaatcata cattaa
//
LOCUS       DANLXS010000127         7049 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.127, whole genome shotgun sequence.
ACCESSION   DANLXS010000127 DANLXS010000000
VERSION     DANLXS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.127"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..598)
                     /locus_tag="PXD71_001918"
     CDS             complement(<1..598)
                     /locus_tag="PXD71_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL6292005.1"
                     /translation="MTELTPKQEAFCFAYIETGNASEAYRQAYETEDMKSETVHRKAK
                     ELMDNGKITARIEELKAEHAERHKLTVDDLLKELEEARILAREKENPNAMTQATMGKA
                     KLLGLDKQVTDPCFDGETGKGDMILSVNCSSIKDGKALDETCILKAGTHEFITRDSFI
                     FYRHIRIDSLDEIKANIEAGYFIQKALINDEAYQQILKG"
     gene            complement(595..1020)
                     /locus_tag="PXD71_001919"
     CDS             complement(595..1020)
                     /locus_tag="PXD71_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292006.1"
                     /translation="MKHELNLAGITALMNEPIKATLSFLDFPHIRIERLHHDNGRPFF
                     IGVLSLDLEDHINIEMAGATGFLEALAIIAYHQQLTATPLLVNDDLLSEINKLPHPQR
                     TNPFIDRHGNEITREQMSKEYREKIDYAFAEKRMAEGKE"
     gene            complement(1027..1179)
                     /locus_tag="PXD71_001920"
     CDS             complement(1027..1179)
                     /locus_tag="PXD71_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292007.1"
                     /translation="MPELLIVLGNRIAKLEQQHNAMPSETDSITLGILRKQLSQLLKV
                     AERIKE"
     gene            complement(1340..2026)
                     /locus_tag="PXD71_001921"
     CDS             complement(1340..2026)
                     /locus_tag="PXD71_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292008.1"
                     /translation="MADHSQFISRLENRIEPKISFQELIQELSNYTNKPFSHIADKLK
                     SLIFSFQQNGIYTNAFVNCTLFEDGESYAIEPKTLHFKDAFDQPKGVLSDVIAQNSID
                     VEHLNAYYVTAREITQLIKTQSPTLLDKLTTKQTQSEISQIQNDYISVYDFIEWASKH
                     YSSLSETANDLNRLLKDKNIQLYRQYGGISPSIDKDNTNLKAAFDFVAINNGYEKQSS
                     SFNFDDDIPF"
     gene            complement(2421..2744)
                     /locus_tag="PXD71_001922"
     CDS             complement(2421..2744)
                     /locus_tag="PXD71_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL6292009.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNDVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
     gene            complement(2857..3075)
                     /locus_tag="PXD71_001923"
     CDS             complement(2857..3075)
                     /locus_tag="PXD71_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module killer protein"
                     /protein_id="HDL6292010.1"
                     /translation="MMREQTTNQIIITDNFSKWLENLKNPIAKATVARRIKNAINGNF
                     GDHKSLAGTGGLYEMRIATGAGYRIYYA"
     gene            complement(3350..4576)
                     /locus_tag="PXD71_001924"
     CDS             complement(3350..4576)
                     /locus_tag="PXD71_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6292011.1"
                     /translation="MARKITPLNDTQIRKAKPEDSPLRDGNGLLLVITSNSKLWRFRY
                     ERPFTKKRNDLSIGAYPDISLAQAREIREEYRALLAQNIDPQTHRQQQEQAKANEISN
                     TYENVAWAWLEHRKTKKNFSDNYQKDVIALIKNNLLPHFGRMPITQITAPMAIKAFKQ
                     YQDEGKLEKLKRAIQKHNEIMMFAVHREMIPFNPTANIAKEFDSPTVEHFKTIKPEDL
                     GEFLYTLQNAQIHLQTRYLILWQLLTMTRPNEAAKAKWADIDEKAKLWTIPAENMKRG
                     IEHKITLSRQAIALLREIKKLSGGKTYLFPSVKNPQTHVNTQTANGAIKRMGYKGKLV
                     AHGLRAIASTYLNEKGYNSDLIEVALSHLNSDRIKAAYDRGERLEQRFKLLQVWADFV
                     EECSQGSLPQYHLKIA"
     gene            complement(4851..5217)
                     /gene="ssrA"
                     /locus_tag="PXD71_001925"
     tmRNA           complement(4851..5217)
                     /gene="ssrA"
                     /locus_tag="PXD71_001925"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5383..6342
                     /gene="rpoH"
                     /locus_tag="PXD71_001926"
     CDS             5383..6342
                     /gene="rpoH"
                     /locus_tag="PXD71_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDL6292012.1"
                     /translation="MKKFNENEIEDAEIIDAEPIDVDEIDEEETSVEAMHPALVSGSM
                     PVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDVEAAKQLILSHMRFVIHIARG
                     YLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVK
                     VATTKAQRKLFFNLRKNKNRLAWFNEEEIKKVADDLGVSVEEVREMESRMTGQDLGFD
                     LPAGDDDEDSYAPSMYIEDSSSNFADDLEDEQHNGQATAQLAYALATLDERSQDIIKT
                     RWLDDNKATLQDLADKYNISAERVRQLENAALKKIKEAISLDG"
     gene            6508..6801
                     /locus_tag="PXD71_001927"
     CDS             6508..6801
                     /locus_tag="PXD71_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HDL6292013.1"
                     /translation="MTQLSTSVYQRADGFINVANAHLKNIAPNQVSNAMLFGCARFNA
                     YVAASKAEYKQQLADSREEVIQYFVEQYKEMLTANLDEYIQNFERYIEGKKAD"
     gene            complement(6819..>7049)
                     /locus_tag="PXD71_001928"
     CDS             complement(6819..>7049)
                     /locus_tag="PXD71_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL6292014.1"
                     /translation="IPASQDFIWDGKDDDERPVTPSEFSIAMKKRGRPAGSKKELVSL
                     RLDKSVLEAFRASGKGWQSRINQVLSDFVKQV"
BASE COUNT         1886 a         1391 c         1471 g         2301 t
ORIGIN      
        1 cgccttttag tatttgttga taggcttcat cattaattag ggctttttga atgaaataac
       61 cggcttctat atttgcctta atctcatcta acgaatcgat tcttatatgg cggtagaaaa
      121 taaagctgtc ccgtgtgata aattcgtgtg ttcctgcctt taaaatacaa gtttcatcaa
      181 gtgctttccc gtctttaatg cttgagcagt ttacacttaa aatcatatct cctttaccgg
      241 tttcaccatc aaagcacgga tcggtaacct gcttatccag ccctagcaac ttcgctttgc
      301 ccattgtggc ttgagtcatt gcgttagggt tttctttctc tctcgccagt atacgagcct
      361 cttccagctc ctttaaaagg tcatcaacgg ttaatttatg gcgttctgcg tgttcggctt
      421 tcagttcctc aatccttgcc gtaatcttgc cgttatccat tagctcttta gcttttcggt
      481 ggacggtttc ggacttcata tcttcggttt cataggcttg gcggtaggct tcgctagcat
      541 tgccggtttc aatataagca aagcaaaaag cctcttgttt tggtgtgagt tcggtcattc
      601 tttcccctcc gccattcgct tttcagcaaa ggcataatcg attttttctc ggtattcttt
      661 gctcatctgc tcccgtgtaa tttcgttgcc gtgtcggtca ataaatgggt ttgttcgttg
      721 tgggtggggg agcttgttga tttcactcaa taagtcatca ttcactaata gcggcgtggc
      781 ggttagctgt tgatgatagg caataatcgc tagagcctct aaaaagcccg tagcccctgc
      841 catttcaata ttgatatgat cttcaaggtc aagagaaagc acaccgataa aaaacggtct
      901 gccgttatcg tggtgcagtc gttcaattcg gatatgcgga aaatctaaaa agctcaaggt
      961 tgctttgata ggttcattca ttaaagcagt aatacccgct aaattaagct cgtgtttcat
     1021 tttggtttat tcctttattc tttccgccac ttttaacagt tggctaagtt gttttcttaa
     1081 aatgccgagt gtgattgaat cggtttcgct tggcattgcg ttatgttgtt gttccagctt
     1141 cgctattcgg ttgcctagca caattaaaag ctcgggcaat gggtaagctc ggtttcagaa
     1201 tagaggctta aaaacgtgaa tagtctgcta aataatcagg tgttatctca gaaaaatcga
     1261 gctttttccc tagtgctgct tctaattctt gtttatttaa aagtaaattt tggaaatcat
     1321 ttaaattcat tgctgccacc tagaacggaa tatcatcatc aaaattaaag ctgcttgatt
     1381 gtttttcata tccgttatta attgctacaa aatcaaaggc tgcttttaaa ttagtattat
     1441 ctttatcaat gcttggactt attccgccat attgtcgata aagctgtata tttttatctt
     1501 tgagtaagcg gtttaaatcg ttagctgttt cactaagact tgagtaatgt ttacttgccc
     1561 attctatgaa gtcataaaca gaaatataat cattttgaat ttggctaatc tctgattgtg
     1621 tttgcttggt ggttaattta tctaacaagg ttggcgattg tgttttaatc agctgtgtta
     1681 tttctcttgc tgttacgtaa taagcattta aatgttcaac atcaatagaa ttttgagcaa
     1741 taacatcact taaaacgcct ttaggctgat cgaaagcgtc tttgaaatgt agtgttttgg
     1801 gctcaatagc atagctttca ccatcttcaa agagagtgca atttacaaaa gcattcgtat
     1861 atattccatt ttgctgaaag gaaaaaatga ggcttttcaa tttatccgct atatgtgaaa
     1921 aaggtttatt tgtatagttt gataattctt gtatcagttc ttgaaagctg attttgggct
     1981 caatacgatt ttcaagccta gaaataaatt gactgtgatc tgccataagc gcccctttaa
     2041 cgtatccctt tactgtgaga gcgtggaaag agaaataaag ggctttctcc tttcgtgccg
     2101 gttaattact ccgccactat ccacgctaat ttgaataaat ccgtatagtt acggattgaa
     2161 cttgataggt tgtattttgc taattattca gataaattac aagtaataag ctgtgtttta
     2221 ttacagtaaa tcaaaatagg ctaaagatgg gtttccggac gctataaaaa agagcattca
     2281 agcggtcata tttcattaag gttttgcgaa tagtgcggaa ttttcggagc agtacgcacg
     2341 caaggagcaa ataaaaagcc gtcaatatcc atcaacggct atttaaaagt gaggttttga
     2401 caggtagcaa gtgataagtt ttagccattt ttgacttgtg gaataagcac taaattgaat
     2461 gcccctagaa ttttttgaat agtgtcaaag cgtggtttgc tgcctttggc tagggtttta
     2521 tataggctct ctctgcctag ccctgtttgc ttggccagct ccgccattcc tcttgctctt
     2581 gctacatcgt tcaaggcttc taaaaattcg tcttggtcgc cctcttttaa cacttccgct
     2641 aaataggctt gaatagttgc ctcatcggtt aaaaactccg ccacgtcaaa agggcgtaaa
     2701 ccgtgctttt ctgctgcctg ttcattgatt tgtattgttt ccattgcttt ctcctgtagt
     2761 ttttaattca gcccataatg ctttcgcttt ctctatatcg gcttgctgtg ttgatttatc
     2821 gccgccgtgt aacaagatat aaattacatt tccttgttat gcgtaataaa ttctatatcc
     2881 ggctcctgtt gctatccgca tttcataaag tccgcctgta ccggctaaag atttatgatc
     2941 gccaaagttg ccattaatcg cattttttat ccgtcttgct accgttgctt ttgcgatagg
     3001 gttctttaaa ttctcaagcc attttgaaaa attatcggta ataataattt gattagtggt
     3061 ttgctctctc atcattgccg gcttcttatt ctatttattg cttattgtat ccattaagat
     3121 acagtgagca agggtttttg ccatttaccc acttttaacc cactttaccc actgtcattt
     3181 tttctttatt tttcagttag ttataaaaaa ataatagtga aaagtgggta aagtgggtaa
     3241 aaatcgaaaa gtttacagaa ttttcatcaa ggggaattac gggcataaaa aagccgtcag
     3301 gcggtttacc taacggcatt acaagcggtc atttttggcg gaaaatttac taggctattt
     3361 tcaaatgata ttgcggtaag ctaccttgcg aacattcctc tacaaaatcc gcccacactt
     3421 ggagcagttt aaaacgttgc tctaggcgtt cgcctctatc ataggcggct ttgattcggt
     3481 cgctgtttaa atgcgataag gcaacctcga ttaaatcgct attgtagcct ttctcattaa
     3541 gataagtgga ggcgatggcc cgcaagccgt gagccactaa tttgcccttg tagcccattc
     3601 gtttgatcgc accgttggcg gtttgagtat tgacgtgcgt ttgtgggttc ttcacgctag
     3661 ggaataaata cgttttaccg ccacttagct ttttaatctc tcgcaaaagt gcgattgctt
     3721 ggcgtgagag tgtgatttta tgctcaatgc ctcgcttcat attttccgcc ggtatcgtcc
     3781 acagtttggc tttttcgtct atatccgccc attttgcctt agccgcttca ttcgggcggg
     3841 tcattgtgag taactgccac agaattaaat agcgtgtctg taaatgaatt tgggcgtttt
     3901 gtaaggtgta taaaaactcg cctaaatctt ccggcttaat ggttttgaaa tgttcaaccg
     3961 ttgggctatc aaactctttc gcaatattgg cggtagggtt aaacggtatc atttcacggt
     4021 gtacggcaaa catcataatt tcattatgtt tttgaattgc ccgttttaac ttctctagtt
     4081 tgccctcgtc ttggtattgc ttgaatgcct tgattgccat tggtgcggtt atttgtgtga
     4141 taggcattct tccaaagtgc ggcaagaggt tatttttaat taaggcgatt acatcttttt
     4201 ggtagttgtc tgaaaaattc ttcttggtct tgcggtgttc cagccacgcc cacgctacat
     4261 tttcataagt gttgctgatt tcgttggcct ttgcctgttc ctgttgttgg cggtgggttt
     4321 gagggtcgat attttgagcc aataatgccc tatattcttc tctaatttct ctcgcttgtg
     4381 ctaaagaaat atcagggtaa gccccaatgc ttaaatcatt ccgctttttt gtgaacggtc
     4441 tttcatacct aaaccgccac aatttagaat ttgaggtaat aacaagtagt aagccgttac
     4501 cgtctcttaa tgggctgtct tccggtttcg cttttcttat ttgggtatcg tttaacggtg
     4561 tgatttttct agccatagta aacctttagg agtataaagc ctaaatctta ggagtatgat
     4621 tttttgtttg ggagtacaaa actaaaagcg ataaagcctt atttgaaaag gctctaaagt
     4681 ggttttagga gtacgaattt ttgggggtat tccggcgtac tcctaaatat actcctaaaa
     4741 tcttcggatt tgattgtatc gtataggtac gcaacggaca ataaaaaagc cttgaaacat
     4801 tgattttcaa ggctttttag gtatcggttg aaccgtatag gattataatt tggtggagct
     4861 ggggggattt gaacccccgt ccgaaatttc tctaccttca gtactacatg tttagtctag
     4921 tctttaattt cgcttattca cagcggacag acacgcaatg aataaactag ctttatttaa
     4981 tttagtgttt cgatcctaaa gcggcggcgt ccacacgatc tcgttttggg tgacttatct
     5041 ttgccctcgt cttacgagcg gaagctgagg agataaggct ttaaagcagg ttattaagct
     5101 gctaaagcgt attgttcgtc gtttgcgact attattttgc ggtttgttta cgaggcctac
     5161 cgcacctcga catgcacctt gggcttcgcc aatcccgtcg aatccaaaat cagccccaaa
     5221 agtagcggta ttctaaagga aagcggtcga atttgcaaaa agttttgcaa gtggcggaac
     5281 ttagcggtat tgaatagctc gaaataaaat catacaaatc ccacaccaga tagatttaac
     5341 tctgttataa tgtaaacctt gctgagagtg aagagaaata gaatgaaaaa atttaacgag
     5401 aacgagattg aagatgctga aattatagat gccgagccga ttgatgttga tgagatagac
     5461 gaagaagaaa catcggtaga agcaatgcat ccggctttgg tttccggctc aatgcctgtg
     5521 ccgcagggca atttggatag ctacatccga atggcaaatc agtatccgat tctcaccgcc
     5581 gagcaagaaa aagaactggc ggagcgttat tattatgatg aagacgttga ggcggcaaaa
     5641 cagctgatcc tttcccatat gcggtttgtg atccatattg ctcgtggcta tttgggctat
     5701 ggtttaccgc tggcagatct gatccaagag ggtaatatcg gcttgatgaa agcggtaaaa
     5761 cggtttgacc cgaatgtcgg cgtgcggttg gtttcttttg cggttcattg ggtgaaagcg
     5821 gaaattcacg aatatgtgtt gaaaaactgg cgaattgtga aagtggcaac caccaaagct
     5881 caacgtaaat tattctttaa tttacgcaaa aataaaaacc gcttggcgtg gtttaatgaa
     5941 gaagagatca aaaaagttgc cgatgattta ggggtttcag tggaagaagt acgagaaatg
     6001 gaatctcgaa tgaccggaca agatttaggt tttgacctgc ctgccggtga tgacgatgaa
     6061 gacagctacg ctccttctat gtatattgaa gatagcagct ccaatttcgc cgatgacttg
     6121 gaagacgaac aacataatgg gcaggcaacc gctcaacttg cctatgcgtt agccaccctt
     6181 gatgaacgta gccaagacat catcaaaacc cgctggttag atgacaataa agccacactg
     6241 caagatttag ccgataaata taacatctcg gcagagcggg taagacagct tgaaaatgcc
     6301 gcattgaaga aaatcaagga agcgatttca ttagacggct agaaatgcct acaagcggtg
     6361 atttttttac tcgctacgct cgcacttcgt gccgtcggca gagccaacgt tcaaacgcaa
     6421 gcgtttgtgc aaaattttac gcaaaaagca ccgcttgtat tttgactaaa aatgaggata
     6481 ctatgattaa cattaccgat aaacaaattg acccaacttt ctaccagcgt ctaccagcga
     6541 gcagacggct ttattaacgt tgccaacgct catttaaaaa acattgcccc aaaccaagtc
     6601 agcaatgcga tgttgtttgg ttgtgccaga tttaacgcct atgttgcggc aagcaaggcg
     6661 gaatataaac agcagttggc cgattccaga gaagaagtta ttcaatattt tgtggagcaa
     6721 tacaaagaaa tgctcactgc aaatttagat gaatatatcc aaaacttcga gcgatatatt
     6781 gaagggaaaa aggcggatta accgcctttg ttttgtggtc aaacttgttt tacaaaatca
     6841 cttaaaactt gattaattct actttgccaa cctttacctg aagctctaaa ggcttcaaga
     6901 acgcttttat caagacgtaa cgaaaccaat tctttctttg aaccagctgg tctgccacgt
     6961 tttttcattg caattgaaaa ctctgatggc gtaaccgggc gttcatcgtc atcttttccg
     7021 tcccaaataa aatcttggct tgccggaat
//
LOCUS       DANLXS010000128         7031 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.128, whole genome shotgun sequence.
ACCESSION   DANLXS010000128 DANLXS010000000
VERSION     DANLXS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.128"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            390..2249
                     /locus_tag="PXD71_001929"
     CDS             390..2249
                     /locus_tag="PXD71_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL6292015.1"
                     /translation="MSNLKVKVQGFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPN
                     ETFAKLVGPMITYLLPLLIGYSGGKLVGGERGAVVGAITTMGIIVGSEIPMFLGAMIV
                     GPLGGWAIKTFDKAIDGKVKSGFEMLVNNFSVGIIGMILAMLSFAFIGGIVTQISAAL
                     GAGVGALVEAGLLPVTSIIVEPAKILFLNNAINHGVFTPLGTAQAQEVGKSILFLIEA
                     NPGPGFGILLAYMFFGRGTAKQTAGSASIIHFLGGIHEIYFPYVLMSPRLILAVIAGG
                     ATGVLTNSLLGGGLIAPASPGSIFAILAMTAPGAHFAVVASVILSCAVTFAIASILLK
                     TQKGEASLEDAQAQTQAMKAESKGVVNGNINKIYVACDAGMGSSAMGASMLRKKVQAA
                     GLNIEVQNLAINDLPQDAQLVITHKDLTARATQRVATAQHLSLTNFLDSAFYDSLVAK
                     LANHQPQQAVQNTQNFANPVEEPQAEGLKLQAAQVFLGLQANSKEEAIRFAGEQLVKA
                     GFVQPSYVDAMFAREQMVSTYLGEGLAVPHGTSDAKDSVLKTGIVVCQYPNGVRFTDE
                     EDGVAKLVVGIAAKGNDHIQILSAITNALDDEQAMKTLTSTNDVEKVLALLSK"
     gene            2353..3492
                     /locus_tag="PXD71_001930"
     CDS             2353..3492
                     /locus_tag="PXD71_001930"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL6292016.1"
                     /translation="MNALHFGAGNIGRGFIGKLLADARIFVTFADINQTQIDQINQNK
                     QYGVKIVGDDSRVEIVKNIAAINSKDENAVIEQVKNTDLITTAVGPNVLGFIAPLFAK
                     ALVARVESGNTQPLNIIACENMVRGTTFFKGKIFEHLTAEQQAEIEKVVGFVDSAVDR
                     IVPPAEPNPADPLEVTVEEFSEWIVDQTQFKGDIPNIKGMELTDNLMAFVERKLFTLN
                     TGHLICAYLGKQAGVKWIKEAIAIEEIKTQVKATMEESGAVLIKRYGFDPQAHSAYIE
                     KILKRFANPYLNDDVNRVGREPIRKLSENDRLIKPLRGTLEYGLPYQNLVKGVVMALQ
                     YRNEEDPQAVELAEFIANHGVAAAVEKYTGLTDQAVIAQVVELYQ"
     gene            3504..4019
                     /locus_tag="PXD71_001931"
     CDS             3504..4019
                     /locus_tag="PXD71_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HDL6292017.1"
                     /translation="MMDYIDKLSEETTLRGFLSIANQRFAKAVEQLIERVFRKTDFAL
                     KSVVDSLFEHQGPLADIEVRLKVLLGLGVISPKVFEDISLFLEVKQHLNEEEDEFAFS
                     HPAVVKFAKDLHHIDFLPINELLNMKASAANKDSMLFQMQQMRLEKMVRSSLILAVTE
                     IDEQLNVESPL"
     gene            4159..6024
                     /locus_tag="PXD71_001932"
     CDS             4159..6024
                     /locus_tag="PXD71_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDL6292018.1"
                     /translation="MQSLLSFVPDFLKQELFWVMALLVFAIWQFIQNKLRMDVVALIV
                     MLFFSLTNILSVKEVLAGLSDPNVVLIALLFIVGEGLVRTGVANQVSDWLMRVSGASE
                     TKVLVLLMLCIAGLGSFMSSTGIVAIFIPVVLAICSGMGISPRRLMMPLSVAGLISGM
                     MTLIATPPNLVAHSELVKSGFEGFNFFSFTPIGLIILVMGIGYMLIARRWLDNGEQSN
                     THSQAQKFSMSQLIEEYQLKERAKMVLVEADSDCVGKSIGQLNLRIEQGLNIIAIQRT
                     KRFRKITVSASVNEIFQPKDILLLDTALDDETYQQRCEQLKLRPIELKGEYFSSQYRS
                     VGMAEISVMPEAECIGKTIRELKFRSRYHLSIVGLKRGDEIIQEELLDTPLKFGDILL
                     VMGVWQQIHRMDDDHRDFFLLRDPAESKNAPPALSQAPHALFSVLTMVLLMITGIVPN
                     VMAALIACLMLGKFRCVDMKSAYDSIHMPSIVLIVGMMPFSIALQKTGGVDLTVRLLL
                     DAMQGLDVHFVLIVLFVVTAIISAFISNTATAILVMPIAIAIANQLGYSPAPFVMTVA
                     VSASAAFMTPVSSPVNTMVLAPAGYKFIDFVKVGVPFTVLVMLVSVFVIPMLFPL"
     gene            complement(6314..6565)
                     /locus_tag="PXD71_001933"
     CDS             complement(6314..6565)
                     /locus_tag="PXD71_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292019.1"
                     /translation="MDKQAILKLFRDNSTAGNSQPSQEPIQVFNNSNVIIQNGDNATV
                     IAGCTVDNNTNLTCADKQRVHDLIHSLEQRNRASRDEGA"
     gene            complement(6577..>7031)
                     /locus_tag="PXD71_001934"
     CDS             complement(6577..>7031)
                     /locus_tag="PXD71_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6292020.1"
                     /translation="FGNRIEQSRKNAKLSRSEVCDALGGIAISTLQAWEADTREPPIS
                     KLMALSQILNVEPCYLLTGERHSQSLNVTREPQPVYATKPQTEEGEVVVIKAKLQEAI
                     ETLEEVLEITQREMKPAGKAQMVWAFYELLTQEKSEKEKMVGLLKLVA"
BASE COUNT         1869 a         1455 c         1660 g         2047 t
ORIGIN      
        1 ctcgaataaa tagatcaatc caataaactg ttgcaaaact aacaaaatag gctccgctct
       61 tctcataaaa cttatatttt ctactcatta tgaacctcta aagtgcagtt tatgctgata
      121 gtaatatagg tgaacagtta gggttagcaa ttcattttat tttttcgatg tagatcgcaa
      181 aaatagaatt atgcttggca caagcgagga cgcttacgcc atctttactc caaaaatgtg
      241 agatagatca cattttcaaa tctgcccaaa aaaatttaat gtgatctact tcacatttct
      301 gagagaaaca tttttgtaac ttttgaaagt ggtgctaatt taagcacgat tttttcaacc
      361 ctaattttct ctcctttttt gaggtttcta tgtctaattt aaaagtgaaa gtccaaggtt
      421 ttgggcgttt cttatcgaat atggtgatgc cgaatatcgg tgcctttatt gcgtggggct
      481 ttatcaccgc cctgtttatt ccaaccggtt ggttgccgaa cgagactttt gctaaactgg
      541 tcggtccgat gattacttac ttgctgccac tgttaatcgg ttacagcggt ggtaaattag
      601 tcggtggtga gcgtggtgcg gttgtaggtg cgattactac aatgggtatt atcgtgggtt
      661 ctgaaatccc gatgttctta ggtgcgatga ttgtaggtcc attaggcggc tgggcgatca
      721 aaaccttcga taaagcgatt gacggcaaag taaaaagcgg ttttgagatg ttggtaaaca
      781 acttctcggt cgggatcatc ggtatgattt tggcaatgct ttcatttgct ttcattggag
      841 gtatcgtcac ccaaatctct gcggcattag gtgcaggtgt gggggcgttg gttgaagctg
      901 gtcttctgcc agtaacctct atcatcgttg aaccggcgaa aatcctattc ttaaacaatg
      961 cgatcaatca cggtgtgttc acgccattgg gtacagcaca agcacaagaa gtgggcaaat
     1021 ctattctgtt cttgattgaa gcgaacccgg gtccgggctt tggtatcttg cttgcctata
     1081 tgttctttgg tcgtggcaca gcaaaacaaa ccgctggtag tgcgtcaatc attcacttct
     1141 taggcggtat tcacgaaatt tatttcccgt atgtgttaat gagcccacgc ttaattcttg
     1201 cagtgattgc aggcggtgca accggcgttt taaccaactc attattaggt ggcggtttaa
     1261 tcgcaccggc ttccccgggt tctatttttg cgattttagc aatgaccgca ccgggtgctc
     1321 actttgcagt tgttgcctcc gtgattcttt cttgtgccgt gacttttgcg attgcgtcaa
     1381 tcttactcaa aacccaaaaa ggtgaagcct cattggaaga tgcacaagca caaactcaag
     1441 cgatgaaagc agaaagcaaa ggcgtagtaa atggtaacat caacaaaatc tatgtagcgt
     1501 gtgatgccgg tatgggttca agtgcgatgg gtgcgagtat gttacgcaaa aaagtgcaag
     1561 cggcaggttt aaacattgaa gtacaaaact tagcgatcaa cgacttaccg caagacgcac
     1621 aactggtgat tacccacaaa gacttaaccg ctcgtgcaac acaacgtgtg gcaacggctc
     1681 aacacttatc gctcactaac ttcttagaca gtgcgttcta tgacagctta gtggcaaaac
     1741 tggctaacca ccagccacaa caagcggtgc aaaatacaca aaattttgca aacccggtgg
     1801 aagaacctca agccgaaggg ttaaaattgc aagcagccca agtcttttta ggcttacaag
     1861 ctaacagtaa agaagaagcg atccgctttg ccggtgagca gttagttaaa gcgggctttg
     1921 ttcaaccaag ctatgtagat gcaatgttcg cccgtgagca aatggtttca acttacttag
     1981 gcgaaggctt agctgtgcca cacggtacat ctgatgccaa agacagcgta ctgaaaaccg
     2041 gtatcgtggt ctgccaatac ccgaacggcg ttcgcttcac cgatgaagaa gacggcgtgg
     2101 cgaaactggt ggtcggtatt gcagcaaaag gcaacgacca tatccaaatt ttaagtgcaa
     2161 ttaccaacgc actggacgat gaacaagcaa tgaaaacact gacctcaaca aatgacgtgg
     2221 aaaaagtgtt agcattatta tctaaataat caccctgttg tgtaggggcg ggtcctgtgc
     2281 ccgcccatta tcctacaaaa tttagggcgg gcacaggacc cgcccctacg ttttatagag
     2341 gaacaatcat caatgaacgc actccatttt ggtgccggta atatcgggcg tggctttatc
     2401 ggcaaattgc ttgctgatgc tcgcattttc gtgacttttg ccgatattaa ccaaactcaa
     2461 atcgaccaaa tcaaccaaaa caagcaatac ggcgtgaaaa tcgtgggcga tgacagccgt
     2521 gtggaaattg tcaaaaacat tgcggcaatt aactcgaaag acgaaaatgc cgtgattgag
     2581 caagtgaaaa acaccgattt aattaccacc gcagtcggcc cgaatgtgct tggctttatt
     2641 gccccactgt tcgccaaagc gttagtggct cgtgtggaaa gtggcaacac ccaaccgctg
     2701 aacattatcg cctgtgaaaa tatggtgcgt ggcacaactt tctttaaagg taaaattttt
     2761 gagcatttaa ctgccgagca gcaagcagaa attgaaaaag tggttggttt tgttgatagt
     2821 gcggtggaca gaattgtacc acctgccgag ccgaaccctg ctgatccgtt ggaagtcacg
     2881 gtggaagagt tcagcgagtg gattgtggat caaacccagt tcaaaggtga cattccaaac
     2941 atcaaaggaa tggaattaac cgataacctg atggcgtttg tggaacgcaa actgttcacc
     3001 ctgaacaccg ggcatttaat ctgtgcctac ttgggcaaac aagcgggcgt gaaatggatt
     3061 aaagaggcga ttgcgattga ggaaatcaaa actcaggtga aagccacaat ggaagagagc
     3121 ggtgctgtgc tgattaaacg ttacggcttc gacccacaag cccattcagc gtatatcgaa
     3181 aaaatcctca aacgttttgc caacccgtac ttaaacgatg acgtaaaccg tgtagggcgt
     3241 gagccaatcc gcaaactgag cgaaaacgac cgcttgatca aaccgttgcg tggcacgttg
     3301 gaatatggct taccgtatca aaacttggtc aaaggcgtgg tgatggcatt gcaatatcgc
     3361 aatgaggaag acccacaagc ggtcgaactt gccgaattta ttgcaaatca cggtgtggcg
     3421 gcagcggtgg aaaaatacac cggtttaacc gaccaagcgg tcattgctca agtggtggaa
     3481 ttatatcaat aattcttgac actatgatgg attacatcga caaactcagt gaggaaacca
     3541 ccctgcgtgg tttccttagc attgccaatc aacgctttgc caaagcggtt gaacagttga
     3601 ttgaacgagt gtttcgcaaa acggactttg cgttaaaatc cgtggtggat tccttgtttg
     3661 aacatcaagg accgctggct gacattgaag tacgtttaaa agtgttgctt gggttggggg
     3721 tgatctcacc taaagtgttt gaagatatct cgcttttcct tgaagtcaaa cagcatttga
     3781 atgaggaaga agacgagttc gcattttctc acccggcggt agtgaaattt gccaaagatt
     3841 tgcaccatat tgattttttg cctatcaatg aattgctgaa tatgaaagcg agtgcagcga
     3901 ataaagattc gatgctcttt caaatgcagc aaatgcggtt ggaaaaaatg gtacgttcaa
     3961 gcctgatttt ggcggtaact gaaattgacg aacaactgaa tgtggaaagc ccattgtaaa
     4021 aatttcgtca aaactaaccg cttgtactga gcattcacgc cgtacgtgat tgctttttcc
     4081 tttctttttg atcttatcgg cttttttctt tgcttaaagc ggtgtaatct atccctaact
     4141 attttggagg atatggttat gcaatcgttg ctctcttttg tgccggattt tcttaagcaa
     4201 gaactgtttt gggtaatggc gttattggta tttgcgattt ggcaatttat ccaaaacaag
     4261 ttgcgtatgg acgtggttgc cttaattgta atgctgtttt ttagcctgac taatatttta
     4321 tcagtcaaag aagtgttggc aggcttaagt gacccaaacg tggtgctgat tgcgttgctc
     4381 tttattgtcg gagaaggttt ggtgcgaacc ggcgtggcga accaagtcag cgattggttg
     4441 atgcgggtgt cgggagcgag cgaaaccaaa gtgctggtgc tgctaatgct ctgtattgcc
     4501 gggcttggat cttttatgag ttccaccggc attgtggcga ttttcattcc ggtggtgctt
     4561 gccatttgct cgggaatggg aatttctcct cgccggttaa tgatgccgtt aagtgttgcc
     4621 ggacttatca gcggtatgat gaccttaatt gccacaccac ccaaccttgt tgctcattcc
     4681 gaattagtaa aatcaggttt tgaaggcttc aatttcttca gttttactcc gattggatta
     4741 attattttag tgatgggcat cggctatatg ctgattgccc gccgttggtt ggataatggt
     4801 gagcaaagca atactcacag tcaggctcaa aaattttcaa tgtcgcagtt aattgaagag
     4861 tatcaactta aagagcgagc caaaatggtg ctggtggaag cagattcaga ctgtgtcggc
     4921 aaatctatcg ggcaactgaa tttacggatt gagcaagggc taaacatcat tgcaattcaa
     4981 cgcactaagc ggtttagaaa aattaccgtc agtgcctcgg taaacgaaat tttccagccg
     5041 aaagatattt tactgcttga tactgccctc gatgatgaaa cttaccaaca acgctgtgaa
     5101 cagctcaaac ttcgcccgat tgagctaaaa ggcgaatact tttcctcaca atatcgctcg
     5161 gtgggtatgg cggaaatttc ggttatgcct gaagcagaat gtatcggtaa aactattcgg
     5221 gaactcaaat tccgctcaag ataccattta agcattgtcg gcttaaaacg tggtgatgaa
     5281 atcatacaag aggaactgct cgatacgcca ctcaaattcg gcgatatttt attggtgatg
     5341 ggcgtatggc aacaaattca ccgtatggac gacgatcatc gagatttctt cttgcttaga
     5401 gatccggcag aaagtaaaaa cgcccctccg gcgttaagcc aagcccctca cgctctgttc
     5461 tcagtgctga ctatggtgtt gctgatgatt accggcattg tgccaaacgt gatggcagcg
     5521 ttgattgcct gcttaatgtt aggcaagttc cgctgtgtgg atatgaaaag tgcttatgat
     5581 tcgattcata tgccaagcat tgtgttgatt gtggggatga tgccgttttc tattgcctta
     5641 caaaaaaccg gtggtgtgga tttaaccgtt aggcttctac ttgatgcaat gcaaggctta
     5701 gatgtacatt ttgttctaat agtattattt gtagtaacag cgattatcag tgcttttatt
     5761 tccaacacag cgactgcaat tttggttatg ccgatagcca ttgcgattgc taaccaactt
     5821 ggctactcac cggcaccttt tgtgatgacg gtcgctgttt cagcctccgc cgcttttatg
     5881 acgccggttt cttcaccggt aaatacgatg gtattagcac cggcaggcta taaatttatt
     5941 gatttcgtta aagttggtgt gccgtttact gtactggtga tgctggtgtc ggtgtttgtg
     6001 attccgatgt tatttccgct ctaaacactc ctctaaatta caagcggtca tttttggtaa
     6061 tctgacgttg acggtaaaat tgttgagtat gtctggcaac atagtgtggg gtactgatct
     6121 tttttgcata gagagaaaga aaaaaatttg ggcggaaaaa taacggcgag gctaggaaat
     6181 acaaggcttc gccgtttttg tttggggtga gttatgcaaa aaacgaaaat gagcaaaaat
     6241 agaaaaaaat gggctatttt tacgttattt tttgcatagg tctgagcagg tttaaacgga
     6301 ttttaaaaca attttaagcg ccctcatctc ggctggctcg gttgcgttgc tctaagctgt
     6361 gaatgaggtc gtgtacacgt tgtttatcgg cacaggttag gttggtgtta ttatccaccg
     6421 tgcagccggc aatcaccgtg gcgttatcgc cgttttgaat gatgacgttg ctgttattaa
     6481 acacctgtat aggctcttgg cttggctggc tgttgccggc agtgctattg tctctgaata
     6541 atttgagaat ggcttgtttg tccatattgt cccctgttat gccaccagtt tgagtaatcc
     6601 caccattttt tctttttcgc ttttttcttg cgtgagaagc tcgtagaaag cccacaccat
     6661 ttgggctttt ccggcaggct tcatttcccg ttgggtaatt tccagcacct cttccaacgt
     6721 ttctattgcc tcttgtaact tagctttgat aacaactact tccccttctt ctgtctgcgg
     6781 ttttgtggca tacacaggct gtggctctct tgtgacatta aggctttgcg agtgtcgctc
     6841 acctgttaat aggtagcaag gctcaacgtt aagtatttgt gacaaagcca taagttttga
     6901 tataggaggt tctcttgtat cagcctccca cgcttgtaat gtagatattg caatacctcc
     6961 tagggcatca caaacctcag atctacttaa ttttgcattt tttctgctct gttctattcg
     7021 gttaccgaaa c
//
LOCUS       DANLXS010000129         6938 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.130, whole genome shotgun sequence.
ACCESSION   DANLXS010000129 DANLXS010000000
VERSION     DANLXS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.130"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(77..382)
                     /locus_tag="PXD71_001935"
     CDS             complement(77..382)
                     /locus_tag="PXD71_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDL6292021.1"
                     /translation="MRKPAHPGQILLDGFIEPNNIKIKELADHLGFSRETLSRVLHGK
                     TPMTANLALSLEEAGISTAKLWLNLQTKYDLWELKQQRLNPVMPFHFGGNYGETAHA"
     gene            complement(391..666)
                     /locus_tag="PXD71_001936"
     CDS             complement(391..666)
                     /locus_tag="PXD71_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6292022.1"
                     /translation="MIKSFKHKGLKQYFEKGITKGIQLNHQRKIDGILDLIDSAESID
                     EFMPFYQCHELKGNRKGIYSMTVNGNWRITFEFVNGDAYILNYEDYH"
     gene            958..1188
                     /locus_tag="PXD71_001937"
     CDS             958..1188
                     /locus_tag="PXD71_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292023.1"
                     /translation="MNAQATLNNHKDYILCGRKEKRTSDFINVFEVFENEATQEFVIE
                     RAMFRNGKLIDWNQSDKMNAEQAQQLWQAYIH"
     gene            1570..1806
                     /locus_tag="PXD71_001938"
     CDS             1570..1806
                     /locus_tag="PXD71_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292024.1"
                     /translation="MSNQEFVKLGSVEPSTIDELQKEDIRQAILKSVSITPYYVGLEI
                     AVCQAISAINKFSDGCITEELLDTKNGDKYRKNN"
     gene            2113..2188
                     /locus_tag="PXD71_001939"
     tRNA            2113..2188
                     /locus_tag="PXD71_001939"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2146..2148,aa:Arg,seq:tct)
     gene            2375..2881
                     /locus_tag="PXD71_001940"
     CDS             2375..2881
                     /locus_tag="PXD71_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292025.1"
                     /translation="MKTKYKIIIERAKQQGLTHIELTQKHCLYLATSVGKLCYGVANN
                     AEQHIKLAAGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELNGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            2885..3244
                     /locus_tag="PXD71_001941"
     CDS             2885..3244
                     /locus_tag="PXD71_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292026.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKQKQDG
                     ILEVIEYRISQIKELLK"
     gene            3241..3720
                     /locus_tag="PXD71_001942"
     CDS             3241..3720
                     /locus_tag="PXD71_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292027.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKAVNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYNFEQIEEDPEQVKPKPSKPKRK
                     PFIPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNVLAEMVKRTQEANNAKHA"
     gene            3854..4066
                     /locus_tag="PXD71_001943"
     CDS             3854..4066
                     /locus_tag="PXD71_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292028.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFILAEENIIELLENNGFETIAT
                     ILAGSDGWRNAINAYLAEQNKHRNEF"
     gene            4079..5029
                     /locus_tag="PXD71_001944"
     CDS             4079..5029
                     /locus_tag="PXD71_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292029.1"
                     /translation="MTTANQQFELILKTESRVVSTNLQTFEEQANQYLATLTTTFETD
                     DDFAKAKEEVKELESIEKKIRDAIKQTQTGEIAELVLTAEQIAERFRQERLTRDKLVK
                     SKETEIKKQIADKAIEEITDTRNKLVKVSDISLALEITMPKHGIANRIAEAQKNKRTI
                     DSLTKAVNAEKALIISEMSVEIGRLTERLEQLAAKSAYLFPDAVKLIATQDDLAPIIQ
                     QRLAEEAEREAAIKAKAEADKVQAEAKAITDKMERKQAVENPQNVAKMNIEEPAQTDG
                     PLGDFIITIRLNQTIQTNAVSIARELKAKFGDSVSLNKAK"
     gene            5070..5864
                     /gene="bet"
                     /locus_tag="PXD71_001945"
     CDS             5070..5864
                     /gene="bet"
                     /locus_tag="PXD71_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDL6292030.1"
                     /translation="MTTALQTLTNKLAERFEMGDGSGLVETLKSSAFAGATVSDAQMI
                     ALLVIANQYQLNPWTKEIYAFPGGNGGLTPIVGVDGWVRIINREPQYDGMEFHFTDDY
                     SACTCTIYRKDRSKPIVVTEFMGECKKSSPAWNSHPKRMLRHKAMIQCARLAFGFTGI
                     YDQDEAERIAENEKPPKNITPQNNVVETTAVELISEEQLSQIRQLMQVTGTEEAKILA
                     YIGVQALNQIPKSQAEAVIKKLNLTLDKQNAEKADNGESVGEEIPL"
     gene            5861..6496
                     /locus_tag="PXD71_001946"
     CDS             5861..6496
                     /locus_tag="PXD71_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL6292031.1"
                     /translation="MTGDLIILDCEQGSEEWLNARLGIPTATGIENIVTPSGKKSSAW
                     NSYLAELVAESIEGAKDGFKSSDMARGNELEPLARMAYEFETDTEVVQVGGVYLNAEK
                     QLMVSPDGLIPNLKKGLEIKCPKMKTHIKYLLEGGVPNEYLIQVQSALWVTGYETWDF
                     VSYCPEYQKQPLYIYTAERDPVLMKAFDQYIPQFIKSLYALKGVTAPKGAF"
     gene            6509..6685
                     /locus_tag="PXD71_001947"
     CDS             6509..6685
                     /locus_tag="PXD71_001947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292032.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAICHFRRTAEYSARATHA
                     TAAECYGGIACTST"
     gene            6706..>6938
                     /locus_tag="PXD71_001948"
     CDS             6706..>6938
                     /locus_tag="PXD71_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL6292033.1"
                     /translation="MKPFDLNKALAGEPVKLRNNDKAFVKYLISDDYIRDNKDHQVQG
                     YTVDEENVFLSEVSWAVSGSHFNDGTIAQYDIVG"
BASE COUNT         2422 a         1409 c         1379 g         1728 t
ORIGIN      
        1 aatattcctt gaataaaccg ccctcgtggc ggttttcttt tcctaagaac aataaaatcc
       61 tccgaagagg gcattattaa gcgtgagcag tttctccata atttccacca aaatgaaaag
      121 gcataacagg atttaaccgc tgctgcttta actcccataa atcatattta gtttgtaagt
      181 ttagccataa cttagccgta ctaattcctg cctcttctaa acttaacgct aaatttgcag
      241 tcattggtgt tttgccgtgt aatactcttg ataaagtttc acgagaaaac ccaagatgat
      301 ctgctaattc tttaattttg atgttatttg gttcaataaa tccatctaat aaaatttgcc
      361 ccggatgtgc tggtttacgc ataactcccc ctaatgataa tcttcataat tcaaaatata
      421 agcatcgcca tttacaaatt caaatgtaat ccgccagttg ccattaaccg tcatagaata
      481 aatgcctttt cgatttcctt ttaactcgtg gcattggtaa aaaggcataa attcatcaat
      541 actttctgct gaatcaatca aatctaaaat accatcgatt ttacgttgat gattaagctg
      601 aatacctttt gttattcctt tttcaaaata ttgttttaag cctttatgct tgaagctctt
      661 aatcataata acacctagca aacacaatgt gatacaaata tatcacgcac aataaaaaag
      721 acaatctttt ttaagtgctt tttctcctaa ttgtctttct cttcttatgt taagtgttta
      781 aaaaacaagc aatcaaacaa cctttctaaa aataatttct ctttaaaatc aattatttaa
      841 tcacgaattg aaatttgttt ggctaaaata attacaaatt gaattgactt aatgatttca
      901 atttgtgata ataaacccat caaaacaaac aacgccacag acaaagagga aaccaaaatg
      961 aacgcacaag caacgctaaa taaccacaaa gactacattt tatgcggacg caaagaaaaa
     1021 cgcaccagtg atttcatcaa tgtgtttgag gtctttgaaa atgaagccac gcaagagttt
     1081 gtgattgaaa gagcaatgtt cagaaatggc aagttaatcg actggaacca aagcgacaaa
     1141 atgaacgccg agcaagctca acaactttgg caagcctaca ttcactaaga atttttatca
     1201 aagcccttca cggagggctt gaataaaggt tctaaacctc gccgaaaggc attgttcttt
     1261 aaaaattaaa ccaaaaacac atcacgacaa gcggattact tagcaatgta atgacattgg
     1321 agagacagaa ccaatctttg cataaagcgg taaaacgtat gcggtaaatg aacttatagg
     1381 caagttgtga aactctctaa gctactcgga ctgaaagaat agtacggtag ttgtggcgaa
     1441 tttactggtg atgtgcgaac tagcggaaag gttaagcact ggcagtgaat cgggaagcac
     1501 aaacgctggt tcaattccaa agcatatttg agaaagtgtg ctttggaata ttaataacag
     1561 gagaacaaaa tgagtaatca ggaatttgtt aaattaggca gcgttgaacc ttcaacgatt
     1621 gatgaattac agaaagaaga tattaggcag gcaatattaa aatccgtctc tataacgcca
     1681 tattatgtgg ggctggaaat agcggtttgc caagcaatat cagccatcaa taaatttagt
     1741 gatggctgta ttacagaaga gcttttagat acgaaaaatg gtgataaata ccgaaaaaat
     1801 aactaatcaa taacaccaaa gtgatttcat tttttcggca ttagccctag atgttgccca
     1861 agtattatat tcctcgaagt tgtggcaaac aagaggactt ggcccggtgg ctcacaagta
     1921 taaaaaagag ccaccatctc taacagctct caacagagag tgagacaaat aaccccgttt
     1981 tagttttaat gttttctaaa gtcggcgtta ggcagcgaaa cgaatgtact agagagttgt
     2041 tagagatggt tagtccatca cattccttag attgaagtaa gccctgccgt tggggttaag
     2101 caacggtact ttgaccgcat agttcagtgg atagagcagc tgccttctaa gcagtgggtc
     2161 gagagttcga atctctctgc ggtcgccatc caaaaccgct ttacgcacat catcgatgac
     2221 gagcctacga agtggaaacc taccgtgtag agcggttttg aatggctaag ttgtgtaact
     2281 gctacaactt aaaaagagcg gttgataata aacataatga ctccgtttcc ccgcctcgtg
     2341 cggggctttt tttatccaca taaaaggatt caaaatgaaa actaaataca aaattattat
     2401 tgagcgagca aaacaacagg gtttgaccca tattgagctt actcaaaaac attgtctgta
     2461 tctcgctaca tcagtaggga aactgtgcta cggggtagct aataatgcag aacaacatat
     2521 taaacttgct gctggagaga tttttacact actaacgatc attaaccatc aaatccagtt
     2581 cgatgaagac ttagagcttc acacgtggaa agctagtgga acaaccccac ccgagcaaag
     2641 agcgatacgt atgttacatc atacctcgca atttgcttgg tttgctcctt ggttagtgga
     2701 cagcaatgaa aaagaacaaa accaaaatac aatcaggatt aatttattta acaaatgctc
     2761 tgggattctg attgaactaa atggcattct taaagaatac gagctaactc ttgatgagtg
     2821 cttagatgaa ttcttgaaaa ataagcattg tttgaatgaa tttagtattc acaaggagta
     2881 aaaaatgacg acgtttagta tttataccag tgtatggcac gacagctcca cgaaaggctt
     2941 tcgccacaac attaagcata aacgccacga ttgctggaga gcggatatcc gcataaagca
     3001 aaaaatcaat gatgtgtggg tgcagatttc aaggatacgc aaacgcttca agtgccctga
     3061 acaggcaaaa tcctttgtag aacaatggaa acaaattgat tcacctgcat ttatgcaaca
     3121 ccaaatcaac cgccaagcat taagagaaga attatcaaac cttgagcaac gcaaacagaa
     3181 gcaagacgga atacttgagg tgattgagta tcgtattagc caaatcaagg agttgctgaa
     3241 atgaaacgca ataaagtaga aagctatact atccgcttat taaaaaataa tcatacaaaa
     3301 cgctacttcg gagaagttat attaaacggc ataaaagaaa tcagcacggc agagatgatg
     3361 tctgaaatta acgctattaa ggcagtaaat aagcgcattg aagctctgaa taaaaccaaa
     3421 gccgttaatt tgcctaatta tcctgaaatc cggggtcatc agcctattat gtacaattac
     3481 aattttgaac aaattgagga agatcctgaa caggttaagc ctaaaccatc aaaaccaaaa
     3541 cgcaagccat tcatacctta tgggctaaac ggctactttg tggatagcaa cggtaatatt
     3601 cgcttacatc tagacagacg agcaagtgcc gaaacaatta cccttgaaac tgatttcttc
     3661 aacgttcttg ccgaaatggt aaaacgtaca caagaggcaa acaatgctaa acatgcttag
     3721 acaatggatt aaatacggct tatacgccct gctttttatg gttgccatca ccctcctaat
     3781 gcaatgaaga gcttatccct gaaagcacct gcaagcctga aacttgcgaa tttaaagtaa
     3841 cggagaaagt gtgatgatag gatacgaaga ctacacagat gcttcacttc aaccagaaga
     3901 agaccctagc gaagaggctt tcatcttggc tgaagaaaac atcatcgagc tattagaaaa
     3961 caacggcttt gaaaccattg ccaccatcct agccggttct gatggctggc gtaacgcaat
     4021 caacgcctac ctagccgaac agaacaaaca cagaaatgaa ttttagagga ataagaaaat
     4081 gacaactgca aatcaacaat ttgaacttat tttaaaaacc gaaagccgag tggtttctac
     4141 caacttgcaa acttttgaag agcaagcaaa tcaatacctt gccacgctca ccaccacctt
     4201 tgaaaccgat gatgattttg caaaagcaaa agaagaagtg aaggagcttg aaagcattga
     4261 gaaaaaaatt cgtgacgcaa ttaaacaaac gcaaactggc gagattgcgg aattggtatt
     4321 aacagcagaa caaatcgctg aacgtttccg ccaagagcga ttaacccgag acaagctcgt
     4381 taagagcaaa gaaacggaaa tcaaaaaaca gattgccgat aaagccattg aagaaatcac
     4441 tgataccaga aacaagctgg ttaaagtaag tgatatttcc cttgccttag aaatcacaat
     4501 gccaaaacac ggcattgcta atcgtattgc cgaggcacag aaaaataaac gcacgattga
     4561 cagcttaacc aaagccgtga acgccgaaaa agcgctgatt atcagcgaaa tgtcggtaga
     4621 aatcgggcgc ttgactgagc gattagaaca gcttgctgca aaatcagctt atctttttcc
     4681 tgatgcagtg aaattgatcg caacccaaga cgacctcgcc ccaattattc aacaacgcct
     4741 tgccgaagaa gccgagcgtg aggcggcgat taaagccaaa gcagaagccg acaaagtaca
     4801 agccgaagcc aaagcgatta ctgataaaat ggagcgcaaa caagcggtcg aaaatccgca
     4861 aaatgtggca aaaatgaaca tcgaagaacc ggcacaaacc gatgggccat taggcgattt
     4921 tatcattact attcgcttaa accaaacaat ccaaaccaac gcagtcagta ttgctcgtga
     4981 gctgaaagcg aaatttgggg atagcgtttc gctcaataag gctaaatgat ttgataccgc
     5041 acaaattcaa cctaaataag gatcatatta tgacaactgc attacaaaca ctaaccaaca
     5101 aacttgccga gcgttttgaa atgggagatg gctcaggatt agttgagaca cttaaatcgt
     5161 ctgctttcgc aggggcaacc gtaagcgatg ctcaaatgat tgcactattg gttattgcta
     5221 accaatacca attaaaccct tggacaaaag aaatttatgc attccccggt gggaacggtg
     5281 gattaacgcc tattgtcggc gttgatggtt gggttcgcat tattaaccgt gagcctcaat
     5341 atgatggaat ggaatttcat tttaccgatg actattccgc ctgtacttgt actatctacc
     5401 gtaaagatag aagtaaaccg attgtggtga cggaatttat gggggaatgt aaaaagagct
     5461 ctccagcttg gaactctcac ccaaaaagaa tgttacgcca taaagcaatg atccaatgtg
     5521 caagattagc ctttggcttt acgggtattt acgaccaaga cgaagcagag cgtattgcgg
     5581 aaaatgaaaa accacctaaa aacatcacac cacaaaataa cgtggtagaa accactgccg
     5641 ttgaactcat ttcggaagaa caactgtcgc aaatccggca gttaatgcaa gtaaccggta
     5701 cggaggaggc gaaaattctc gcctatattg gcgttcaagc actgaaccaa atccctaaat
     5761 cgcaggcaga ggcagtgatt aaaaagctca atcttacgct agacaagcaa aacgctgaaa
     5821 aggcggataa tggcgaaagt gtcggtgagg aaatcccact atgacaggcg atttaatcat
     5881 tttagattgc gaacaaggat cagaagaatg gctcaatgcc agattgggca ttcccaccgc
     5941 cacaggcatt gaaaatatcg ttacgccaag cggtaaaaaa tcgtccgctt ggaatagcta
     6001 tcttgccgaa ctggttgccg aaagcattga gggagcaaaa gacggtttta agtcgtctga
     6061 tatggcacga ggcaacgagc ttgaaccgct tgcacgaatg gcttacgagt ttgaaaccga
     6121 caccgaagtc gtgcaagtcg gcggtgtgta ccttaacgcc gaaaaacagt taatggtaag
     6181 tcctgacggc ttaattccca atctaaaaaa agggttggaa atcaaatgcc cgaaaatgaa
     6241 aacccacatc aaatacctgc tagaaggcgg tgtgccgaat gaatatctta ttcaagtgca
     6301 atctgccctt tgggtaacag gctacgaaac gtgggacttt gtcagctact gccccgaata
     6361 ccaaaaacaa ccgctttaca tctacacagc cgaacgtgat ccggtattga tgaaagcctt
     6421 tgatcaatat atcccgcaat ttatcaaatc cctttatgca ttgaaggggg taaccgcccc
     6481 gaaaggggct ttttaatagg tagccaatat gacaacccaa aagaaatacg aactactcca
     6541 aaacgacaca atcaaccaca acggcagaac actctaccgc atcaaagcca tatgccactt
     6601 taggcggacg gctgaatatt ccgcaagagc cactcacgcc acagccgctg aatgctatgg
     6661 tggaattgcc tgtacatcaa cataaccaca aacacggaga ataaaatgaa accatttgat
     6721 ttaaataaag cattggcagg tgagccagta aaactaagaa ataatgataa ggcatttgta
     6781 aaatacctaa ttagtgatga ctatattaga gataataaag accaccaagt acaagggtat
     6841 acagttgatg aagaaaatgt atttttgtca gaagtatcgt gggctgttag cggatctcat
     6901 ttcaatgatg gaacaatagc tcaatatgat attgttgg
//
LOCUS       DANLXS010000130         6767 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.131, whole genome shotgun sequence.
ACCESSION   DANLXS010000130 DANLXS010000000
VERSION     DANLXS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.131"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(23..1156)
                     /locus_tag="PXD71_001949"
     CDS             complement(23..1156)
                     /locus_tag="PXD71_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL6292034.1"
                     /translation="MKTIKIGLVGTGYIGRCHAIAYAQAPTLFPLKGKIQLEYLAEIN
                     QALADQKAREFGFQRATDDWRKLVTDPNVDVVDICTPNFLHKEIALEAIKHGKHVYSE
                     KPLALTAQDAKEMVIAAQQAGVKTLVGFNYIKNPTTQLARQIIQNGEIGEVVHFYGTH
                     NEDYLANPNTPIDWHCYKAKAGLGTLGDLAAHIVSIAHYLVGSDIERVIGDMQTVIEE
                     RPSPHNPAEKIKVENEDQASSLIRFANGVMGTLESSRISCGRKMGLTYVVTGTKGTLS
                     YTQERMAELKLYLHDDDKSRQGFKTILVGPEHPDYAAFCVSAGHGIGFNDQKAVEIRD
                     LINGIAADDPMYPDFEEGYKISRVLEAIAKSAEEKRWVEVSEI"
     gene            complement(1287..2315)
                     /locus_tag="PXD71_001950"
     CDS             complement(1287..2315)
                     /locus_tag="PXD71_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6292035.1"
                     /translation="MTTQFLKNMGMVDDKGKIDFIAFFERFGVLIFLILLIIFFTSQN
                     GAFLSQRNIYNVLTEVSIFGIMAVGMTFVILTAGIDLSVGSILAVTAMFAAYIIKGDN
                     TVTLEAGAWGGMSWLIGLSICLGLGTFIGWLHGLGVTKLKLPPFIITLGGMTIWRGLT
                     LVLNNGSPIAGFDEGYRWWGRGDILGVPVPVVIFAVVAIIGYFALHKTRWGRYVYSVG
                     GNPEAARLAGVNINRTLVSVYVVIGALAGLAGFILSARLGSAESVAGVSFELRVIASV
                     VIGGTSLMGGYGRVTGTIIGSIIMGVLINGLVLMDVSAYYQQIIMGLIIILAVAFDTY
                     AKSRRGAL"
     gene            complement(2327..3871)
                     /locus_tag="PXD71_001951"
     CDS             complement(2327..3871)
                     /locus_tag="PXD71_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6292036.1"
                     /translation="MSTPLLEVKDLTKSFSGVMALNGVKLTVGKGEVHALLGENGAGK
                     STLLKALSGAQPQTSGEIIFNGETLSLSDSPFDRQLKGIVTIYQEFNLLPNMTVAENF
                     FLGREPVKGLFVNEKAVNEEAQLVLDNLGLNIKPDTQVSRLSVAQQQMVEIARAMTLN
                     AKLIIMDEPSAALSDKEVETLHQIVRDLKSRGISVIYVTHRLNEVFALCDRFTVFQDG
                     RYSGEGEVKNVNVDQIIKMMVGREVAFTRRPPEETHHTHRPIRLAVKNLHREKPAFDP
                     HGIALHGVSFNIHEGEVLGIAGLVGAGRTEIARCLFGVENYTADEISLDGKPYAATSP
                     LDALEKGVALVPEDRKKEGLVLGLPIKTNMTLPILSKLLKGNLFVDNQKEDDLIETYR
                     QALRIKMASSELEARKLSGGNQQKVILARCMALNPKVLIVDEPTRGIDVGAKSEVHQV
                     LFDMAKQGVAVLVISSDLPEIMAISDRIITLSEGRVTGEIHGDDATEEKLMSMMAVGV
                     QRKAAA"
     gene            complement(3922..4860)
                     /locus_tag="PXD71_001952"
     CDS             complement(3922..4860)
                     /locus_tag="PXD71_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL6292037.1"
                     /translation="MKKRIKFVLSALTLGLAMNSFAKDELVVFSLPNLSSPFEVQLQK
                     VAVETSKKLEIKLQVLDGQSSSTKQASDLENAITRGAKGIIISPNDVNAISGAVEEII
                     KEKIPAATLDRKVESSKPVPHFGANNYTGGQEVAKAVKAKYPNGAKIILLTGQPGSTS
                     NIERTKGIRDELAAGGDKYKIVVDQTGNWLRSEGLRIIESVLPTLKEKPEVIISANDD
                     MALGAIEALRSQGLKAGDILVTGFDSTPEALARVKDGWLYLTADQRPSFAVSTALEQV
                     VGNIRESKEVSGADYPPKIILKDNLQEAERFAEISE"
     gene            complement(5192..6703)
                     /locus_tag="PXD71_001953"
     CDS             complement(5192..6703)
                     /locus_tag="PXD71_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL6292038.1"
                     /translation="MSQFVQNFINGAVVESQSQRVTSVFNPATGEETKKVKLSLPNEV
                     DAAIAAADQAFKSWSQQSPLRRARILFKFKELLEANFDELARLISSEHGKIYSDAIGE
                     LTRGLEVVEFATGIPHLQKGEFSANAGRGIDIHSIQQPLGVVAGITPFNFPAMVPMWM
                     FPVALACGNTFVLKPSEKDPSVSIRLAELLKEAGLPDGVFNVVQGDKEAVDILLTDSR
                     IQAVSFVGSTAIAQYIYEKGSANGKRVQALGGAKNHALIMPDADVQGTINALLGAAFG
                     AAGERCMALSVAVVVGDELADKIIEELIPKVKALKIGSGLLPQGVLENDMGPVISKEH
                     KAKIESYIDQGVAQGAKLCVDGRGYKVEGFENGYFVGGTLFDNVTPEMTIWQDEIFGP
                     VLSVVRVKDYAEGIALINSHQYGNGSAIFTADGDSARQFTQDVQAGMVGVNIPIPVPM
                     AFHCFGGWKASIFGPLNVYGTDGVRFYTRMKTITTRWPDSSVRSQAAFNFPTL"
BASE COUNT         1805 a         1623 c         1422 g         1917 t
ORIGIN      
        1 cgtttctatt ttaatgtaat tattaaatct cactcacctc cacccacctt ttctcctccg
       61 ccgattttgc tatcgcttcc aatactctgg agattttata gccttcttca aaatcgggat
      121 acatcgggtc atcagcggcg atgccgttga tgaggtcacg gatttccacc gctttttggt
      181 cgttaaaacc gatgccgtga ccggcactca cacaaaaagc agcgtagtcg ggatgttccg
      241 ggccgactaa aatggttttg aatccttggc gggatttatc gtcatcgtgc aaatagagtt
      301 tgagttctgc cattcgttct tgggtatagc tgagcgtgcc tttagtacct gtaacgacat
      361 aggtcagccc cattttacgc ccgcaggaaa tgcgggagct ttcgagcgtg cccattaccc
      421 cattagcaaa tcgtatgaga ctacttgcct gatcttcgtt ttctactttg attttttctg
      481 ctggattgtg cggactaggg cgttcttcaa tcactgtttg catatcgccg ataacacgct
      541 caatgtctga accaaccaaa tagtgtgcga tactgacaat atgtgcggct aaatctccca
      601 acgtaccaag cccggctttg gctttgtagc agtgccaatc aatcggggta ttagggttgg
      661 cgagataatc ttcgttatgc gtgccgtaga aatgcaccac ctcaccaatt tcgccgtttt
      721 gaataatttg gcgagccagt tgggtggttg gatttttgat atagttgaaa ccgaccaagg
      781 ttttaactcc cgcttgttga gcggcaatca ccatttcttt ggcatcttgg gcggtgagtg
      841 caagcggttt ttctgaatag acgtgtttgc cgtgtttaat cgcttctaag gcaatttctt
      901 tgtgcagaaa atttggtgta caaatgtcca ccacatcgac attcggatcg gtaaccaatt
      961 ttcgccaatc gtcagtagca cgctgaaagc caaattctcg ggctttttga tcggctaatg
     1021 cttggttgat ttcggcaagg tattccagtt ggattttgcc tttaagtgga aacagggttg
     1081 gggcttgagc gtaggcaatg gcgtggcagc gaccgatata gccggtgcca actaagccga
     1141 ttttgatggt tttcataata ctatcccagt agggtgcgtt tgctaacgca ccattagaat
     1201 tttcagtgca ttggcaaacg caccctataa gaaagcggtc tggttttgca aaatttttac
     1261 aaaatcagac cgcttgtgtt agctatctat aatgctcctc gtctgctctt cgcataggtg
     1321 tcgaaggcaa cggcaaggat gatgattaag cccataatga tttgttggta gtaagcggaa
     1381 acgtccatta gtactaaacc gttaatcaat acacccataa taatggagcc gatgatggtg
     1441 cctgtgactc gtccgtatcc ccccattagg gaagttccgc cgataaccac tgaggcaatt
     1501 acacgtagct caaaagaaac gccggcaacg gattcggcac tgcctaaacg ggcggagaga
     1561 atgaaaccgg caagtcctgc caatgctccg atcaccacat aaacactgac taaggtacgg
     1621 ttaatattca caccggctaa acgggctgct tccggattgc cacctaccga gtacacatag
     1681 cgtccccagc gggttttatg caatgcgaag taaccgataa tggcgactac ggcaaaaatc
     1741 actaccggca ccggtacgcc taaaatatcg ccacgtcccc accagcggta accttcgtca
     1801 aatccggcaa ttggcgagcc gttatttaac acaagtgtta aaccacgcca aattgtcata
     1861 ccgccaagcg tgataataaa cggtggcagt ttgagtttag tgacgcctaa gccgtgtaac
     1921 caaccaatga aagtgcctaa acctaaacag atactcaaac ctatcagcca gctcattccg
     1981 ccccaagcac cggcttctag cgttaccgta ttatcgcctt tgataatata ggccgcgaac
     2041 attgcggtaa cagcaaggat tgagccgaca gagagatcga tccctgcagt taagatgacg
     2101 aaagtcattc ctaccgccat aatgccgaaa atcgacactt cggttaaaac gttatagatg
     2161 ttgcgttgcg ataaaaatgc cccgttttgt gaagtgaaga aaataatcag taagatcaag
     2221 aaaatcagta cgccgaaacg ttcaaagaag gcgataaaat cgatttttcc tttgtcatcg
     2281 accataccca tattttttag aaattgcgtt gtcatacaaa cctctcttat gctgccgctt
     2341 tacgctgaac gccaacagcc atcattgaca ttaatttttc ttcggtggca tcatcgccgt
     2401 gaatttcgcc agttacccga ccttcggaaa gagtgataat gcggtcggaa atcgccataa
     2461 tttccggtaa atcggaggaa atcactaata ccgcaacacc ttgttttgcc atatcaaaca
     2521 gcacttggtg aacctcagat tttgccccaa catcaatacc tcgtgtcggc tcatcaacaa
     2581 ttagtacttt cgggttgagt gccatacaac gggctaaaat cactttttgc tgattaccac
     2641 ccgataattt gcgggcttca agttcgctac ttgccatttt gatgcgtaac gcttggcggt
     2701 aggtttcgat taaatcatct tctttttgat tatcgacaaa caagttaccc ttcaataatt
     2761 tgcttaaaat cggcagggtc atattggttt taatcggtaa accgagaact aagccctctt
     2821 ttttgcgatc ttccggcaca agggcaaccc ctttttccaa agcatctaac ggcgaggtgg
     2881 cagcataagg cttaccgtct aggctgattt catcagccgt ataattttct accccaaaca
     2941 agcaacgggc aatttcagtt cgccctgcgc caactaagcc ggcgatccct aatacttcac
     3001 cctcgtgaat gttaaaagag acaccgtgga gagcaatgcc gtgtggatcg aatgccggtt
     3061 tttcccgatg cagatttttc acagctaaac gtattggtcg gtgggtatgg tgagtctctt
     3121 ctggtggacg gcgagtaaag gcaacttcac gtcctaccat cattttaata atttgatcga
     3181 cattcacatt tttgacttca ccctcaccag aatagcgccc gtcttggaaa acggtgaaac
     3241 ggtcgcatag ggcaaacact tcgtttaagc ggtgggtcac ataaatcacg ctaatgccac
     3301 ggctttttaa atcacgcacg atttggtgta gtgtttccac ttctttatca cttaaggcag
     3361 cggacggttc gtccataata atcaacttag cgtttaaggt cattgcacgg gcaatctcga
     3421 ccatctgctg ttgtgccaca cttaaacggg aaacttgggt gtcaggtttg atatttaagc
     3481 cgagattatc cagcactaat tgtgcttcct cattcaccgc tttttcattg acgaataaac
     3541 ctttcaccgg ctctctgccc aaaaagaaat tttcagcaac cgtcatattc ggtaacaagt
     3601 taaattcttg ataaatcgtt acgatgcctt tgagttggcg atcaaacggg gaatcgctca
     3661 aacttaaggt ttcaccgtta aagataattt cgccacttgt ttgtggctga gcgcctgaaa
     3721 gtgcttttaa taaggtcgat tttcctgcac cgttttcacc caacaatgcg tgaacttcgc
     3781 cttttcccac agtcagtttc acgccattga gtgccataac accagagaaa ctttttgtca
     3841 gatctttcac ttcaagtaag ggagtagaca tacaacctcc gtaaaaaaac gccctccggc
     3901 ctcagccgga aggcagataa attattcgct gatttctgca aaacgttctg cttcttgcag
     3961 attatctttc aggataattt ttggcggata gtccgcaccg ctcacttctt tgctctcacg
     4021 gatattgcca accacttgct ctaatgcggt gcttaccgca aaacttgggc gttggtcagc
     4081 ggtgagatac aaccagccat ctttcacacg tgcaagagcc tcaggggttg agtcaaagcc
     4141 ggttactaaa atatcgccgg ctttcaaacc ttggctacgc aatgcttcaa tcgcaccaag
     4201 tgccatatcg tcattcgctg aaataatcac ttctggtttc tcttttaagg ttggtaacac
     4261 actttcaata atgcgtaatc cctcagaacg gagccagtta ccggtttgat caaccacgat
     4321 tttatatttg tcaccaccgg cagctaattc atcacgaata cctttggtac gttcaatgtt
     4381 agaggttgaa cccggctggc cggttaaaag aataattttt gccccattcg ggtatttggc
     4441 tttcactgct tttgcgactt cttgaccgcc tgtgtagtta tttgccccaa agtgtggaac
     4501 cggcttactg ctttcaactt ttcggtctaa tgtcgcagca ggaatttttt ctttgataat
     4561 ttcttccact gcacctgaaa tcgcattcac atcattcggg gaaatgataa tgcctttagc
     4621 accacgggta attgcatttt ctaagtcaga agcctgctta gtagaagaac tttgcccgtc
     4681 taatacttgc aatttgatct ccaacttttt actggtttct accgctactt tttgtagttg
     4741 aacttcaaac gggctggata ggttcggtaa gctaaatacc acaagctcat ctttcgcaaa
     4801 gctgttcatt gctaagccta acgttaaagc actcaataca aatttaatac gctttttcat
     4861 tacaaaatcc tctttttaag atgggaattg acggcgaata ttgccaatgt tacttcaaac
     4921 tgcgaaggaa acttaagtct tatttcaata ataaacctca atgaaataaa tttttcaatt
     4981 tagaatatat cattcaaaat tttccaaacc aaatgctttg tttcaaattt gttacgtaga
     5041 tcacattttt aggaagagat aaatgcaagc ggtcaaaaca ggtcgctttt ttgcaaaaat
     5101 ttgaataaaa atgaccgctt gtgagatgaa aaccaataaa aaagcggtga tttctcaccg
     5161 ctaaacactc aaaaaagaaa tgtactcgtt attataaggt tgggaaatta aatgccgctt
     5221 ggctacgcac cgagctgtcc ggccaacggg ttgtgatggt tttcatacgg gtgtagaaac
     5281 gcacgccgtc tgtgccgtac acattgagag gcccgaaaat agaggctttc caaccgccaa
     5341 agcagtggaa cgccattggc acaggaattg gaatattcac gcccaccata ccggcttgta
     5401 catcttgggt gaattgacgt gctgaatcgc catctgcggt gaaaattgca ctaccattgc
     5461 cgtattggtg actgttaatg agtgcgatcc cttcggcata atctttcaca cgcacaacag
     5521 agagaaccgg tccgaagatt tcatcttgcc agatggtcat ttcaggtgtc acgttatcaa
     5581 acagcgtacc tccgacaaag taaccgtttt caaacccttc gactttatag cctcgaccat
     5641 cgacacagag tttcgccccc tgtgccaccc cttgatcgat atagctctca attttggctt
     5701 tatgctcttt ggaaatcacc ggccccatat cattttcaag cacgccttgt ggtagtaagc
     5761 ctgaaccgat tttcaatgct ttgaccttag gaattagctc ttcaatgatt ttatcggcta
     5821 attcatcacc aacaactacc gctaccgata gtgccataca acgttcaccg gctgcaccaa
     5881 atgctgcgcc cagtaaggca ttaattgtgc cttgtacatc agcatccggc ataatcaaag
     5941 cgtggttttt cgcaccgccc aaggcttgaa cccgtttgcc gtttgctgag cctttctcat
     6001 aaatatattg tgcaattgca gttgagccga caaaactcac cgcctgaatg cgagagtcgg
     6061 ttaaaagaat atctaccgct tctttatcac cttgcaccac attgaataca ccgtccggca
     6121 agccggcttc ttttaataat tctgccaagc gaatagacac acttgggtct ttttcagacg
     6181 gttttaaaac gaaagtgtta ccgcaagcaa gtgccactgg gaacatccac atcggtacca
     6241 ttgccgggaa attgaacggg gtaatgcctg ccactacgcc caacggttgc tggattgagt
     6301 gaatatcaat gccacgccct gcattggcgg agaactcacc tttttgtaag tgtggaatac
     6361 cagtggcaaa ttccaccact tctaaaccac gggttaattc gccaatcgca tcggaataga
     6421 ttttgccgtg ttctgagctg attaaacgag ccaactcatc aaagtttgct tccaataact
     6481 ctttgaattt aaataaaatt ctggcacggc gtagtggtga ttgttgtgac cagcttttga
     6541 aagcctgatc ggccgctgca attgccgcat cgacttcgtt tggcaggctt aatttgactt
     6601 ttttggtctc ttcaccggtt gcgggattaa ataccgaggt aacacgctgg ctttggcttt
     6661 caacgactgc accgttaata aaattttgga caaattggct cattggaggc tccttatcaa
     6721 ctgattgaga ggaaaaacgg ataaaataca gccgataaac taccgct
//
LOCUS       DANLXS010000131         6712 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.132, whole genome shotgun sequence.
ACCESSION   DANLXS010000131 DANLXS010000000
VERSION     DANLXS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.132"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..200)
                     /locus_tag="PXD71_001954"
     CDS             complement(<1..200)
                     /locus_tag="PXD71_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292039.1"
                     /translation="MITSNLERSFHGLNIAQHNYKIFLDESGGNIGKLKHTPNDKKGS
                     NFNRTISSHIGVPPVFVLGGIA"
     gene            complement(227..901)
                     /gene="cas4"
                     /locus_tag="PXD71_001955"
     CDS             complement(227..901)
                     /gene="cas4"
                     /locus_tag="PXD71_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDL6292040.1"
                     /translation="MGDLQNSAEILPLVQPKFGDDKRSISLSALQHYAFCPRQCALIH
                     NEQVWAENYLTAQGQALHERVDSGEPEVRKGIRFERTVHVAAEKLGISGILDLVERDL
                     KTGELKPVEYKRGKPKLDPIDEIQLCAQALCLEEMTEQTINEGALWYMQTRHRLPIIF
                     STELRTKTLATIAEVRSLLDSGTTPPPEYSKRCKACSLIDLCQPKLLERDRSKGYVVG
                     VFGSLL"
     gene            complement(901..1764)
                     /gene="cas7c"
                     /locus_tag="PXD71_001956"
     CDS             complement(901..1764)
                     /gene="cas7c"
                     /locus_tag="PXD71_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HDL6292041.1"
                     /translation="MSIQNRYEFVFFFDVTNGNPNGDPDAGNMPRLDPETSKGLVTDV
                     CLKRKIRNFIEMSYENEAGYEIYVKEKSVLNLQNKRAYEALGIESEAKKLPKEEAKAR
                     EITAWMCKNFFDIRTFGAVMTTEVNSGQVRGPVQLAFAQSIDPIIPLEVSITRMAVTN
                     EKDLEKERTMGRKYIVPYALYRVHGFISAKLAEKTGFSDEDVQKLWQALQLMFEHDRS
                     AARGEMAARKLIVFKHDSELGNQPAHKLFDSVKVERINGEKDTPAKDYDDYCISVQTE
                     GLTGVTVEELL"
     gene            complement(1774..3555)
                     /gene="cas8c"
                     /locus_tag="PXD71_001957"
     CDS             complement(1774..3555)
                     /gene="cas8c"
                     /locus_tag="PXD71_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HDL6292042.1"
                     /translation="MITALTRYYYRLAAQEDRLTGEAKVPSYGFSEEKIGWVLELDSK
                     GNLVNVISNLTEDKKPKPKLMSVPRPEKRTSGIKPNFLWDKTAYVLGIESNPDKATAK
                     DQPINFAEKTFEAFKQYHLDLLADSNDEGLMAIRNFLQQWQPTHFAESICPREILDAN
                     VVFKLVGTSGYIHQREAAQTLWTSLLINQSDNAEKGLCLISGKLSPIQRTHPPIKGVF
                     GGQSSGGSIISFNKESFTSFGKEQGSNAPVSEQAAFAYTTALNYLLRTEKHRLTIGDA
                     STVFWAEADNTTQAEAAEGFFAALMTPPDDEQENQKIFEELQKISKGRPLAEISPELS
                     ENTRFYVLGLAPNAARISVRFWLDTTFGQLAKNLSEHWQDLALEPCPWKTPPSIWRLL
                     LQTASLGKSENISPVLAGEMARAVISGHLYPMSLLSQLVTRIRADGDINGLRVALMKA
                     VLQRKFRKGFIKEGVPMSLNKESDNQAYLLGRLFAVLERIQSQALGELNAGIADRYYG
                     SASAVPFSVFPRLLSGAKHHLSRLRKDKGGMAVNLDKDLGEIIASLPENFPRHLSIDE
                     QGRFAIGYYHQKQSYFAKKETTESIEN"
     gene            complement(3577..4254)
                     /gene="cas5c"
                     /locus_tag="PXD71_001958"
     CDS             complement(3577..4254)
                     /gene="cas5c"
                     /locus_tag="PXD71_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HDL6292043.1"
                     /translation="MPNKIRLHIWGDYACFTRPEMKVERVSYDVITPSAARGILAAVH
                     WKPAIRWVIDKIYVLQPIQFESVRRNELGSKISASKISGAMKRKTVADLYTVIEDDRQ
                     QRAATVLKNVGYVIEAHAVLTKMAGEAETVTKHIEMFKRRAKKGQCFQQPCMGVREFA
                     ANFALIDDDEPLPECFLAENERDRDLGWMLHDIDFEHGNQPRFFRAEMKNGVIDVPPF
                     YAEEVKS"
     gene            complement(4496..6247)
                     /locus_tag="PXD71_001959"
                     /pseudo
     CDS             complement(4496..6247)
                     /locus_tag="PXD71_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250669.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(6237..6665)
                     /locus_tag="PXD71_001960"
     CDS             complement(6237..6665)
                     /locus_tag="PXD71_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292044.1"
                     /translation="MAGDVPMTSCIGKVEEPLIITPGVGKYKKGYGQPFDAHREKANE
                     AIDSASSILCIGYGFNDDHLQTHLTGKIKSGVKTLLLTRGLSENARNVVKEAPNCKAL
                     IFDGDKAGTRVVSKQEETFIPNVHWWDVEFFVKEILENGK"
BASE COUNT         1863 a         1541 c         1271 g         2037 t
ORIGIN      
        1 tgagcaatcc ctcctaatac aaatacagga ggaaccccaa tgtgggaaga aatggttcta
       61 ttaaaattac tacctttttt atcattaggc gtatgtttta acttcccaat atttccacca
      121 ctctcatcaa gaaatatttt gtagttatgc tgtgctatat ttaatccgtg aaatgatctt
      181 tctaagtttg aagttatcac aacaagtccc tccccaaagt aggtaattat aataaagatc
      241 caaacactcc caccacatac cccttcgatc tatccctttc caacaacttt ggttggcaga
      301 ggtcgataag cgagcaggct ttgcagcgtt tgctgtattc tggaggaggt gtggttccgc
      361 tatctaagag cgaccgcact tcggcaatgg tggcgagggt tttggtgcgt agttcggtgg
      421 aaaaaatgat cggcaaacgg tggcgagttt gcatatacca caacgcccct tcgttaatcg
      481 tctgctctgt catttcttcc aaacataagg cttgggcaca gagttggatt tcatcaatcg
      541 gatcaagttt gggcttgcca cgtttgtatt ccactggttt tagctcgcct gtttttaggt
      601 ctcgctcaac caaatccaaa atgccgctta tgcctaattt ttcggctgca acgtgaaccg
      661 ttcgctcaaa gcgaatccct ttgcgaactt ctggctcacc actatctacc cgctcgtgca
      721 acgcttgacc ttgtgcggtg agatagtttt ctgcccacac ctgttcgttg tggatcagcg
      781 cacattgacg tgggcagaag gcatagtgtt gcaaagcgga aagcgaaatt gaccgcttgt
      841 catcgccaaa ttttggctga acaagcggta aaatttccgc agaattttgc aaatcgccca
      901 ttacagtaat tcttcaacgg ttacacccgt taagccttcg gtttgtacgc tgatacagta
      961 atcgtcatag tctttcgctg gcgtgtcttt ttcaccgttg attcgctcaa cttttaccga
     1021 gtcaaacagt ttgtgtgcag gttggttgcc taattcgctg tcgtgtttaa atacgatcag
     1081 tttacgagct gccatttcgc ctcgtgcggc agaacggtcg tgttcaaaca taagttgcaa
     1141 ggcttgccaa agtttttgta catcttcatc agaaaagccc gttttttcag cgagtttagc
     1201 tgaaataaag ccgtgaacac gataaagtgc gtaaggcacg atatatttac gccccatagt
     1261 ccgttctttt tcaagatctt tttcgttggt cactgccatt cgtgtgatcg atacttctag
     1321 cggaataatc ggatcgatag attgagcgaa cgccaattgc actgggcctc gcacctgtcc
     1381 gctgtttact tcggtagtca ttaccgcccc aaaggtgcga atatcgaaaa agtttttgca
     1441 catccaagcg gtaatttctc gggctttagc ttcctctttt ggtagttttt tcgcttccga
     1501 ttcaatccct aatgcttcat aagcacgttt gttttgtaaa ttcagaacac ttttttcttt
     1561 aacataaatt tcgtaacccg cctcgttttc atagctcatt tcaataaaat tgcggatttt
     1621 gcgttttagg caaacatcgg tgactaaacc tttactggtt tcaggatcaa ggcgtggcat
     1681 atttcctgca tctggatcac catttgggtt accgttggtt acatcaaaga aaaaaacaaa
     1741 ttcatagcgg ttttgaatag acatagtgtt tccttagttt tcaatagact cagtggtttc
     1801 ttttttagcg aaatagcttt gtttttggtg gtaataacca atcgcaaaac gcccttgttc
     1861 atcgatactt aaatggcgag ggaaattttc cggcaagctg gcaataatct cacctaaatc
     1921 cttatccaga ttaaccgcca ttccgccctt atcttttctc aagcgagata aatggtgttt
     1981 tgcaccgctt aataagcgag ggaataccga aaacggcacg gcagaagccg aaccataata
     2041 gcggtcggca atgccggcat ttaattcgcc taatgcttgg ctttggattc tttctaatac
     2101 tgcaaataac ctgccgagca agtaagcttg gttatcgctt tctttattta agctcatagg
     2161 aactccttct ttaataaaac cttttctaaa ttttcgttgt aagactgctt tcatcaacgc
     2221 tactcgcaaa ccgttaatat cgccgtctgc tcggattcga gtcactaact gtgaaagtaa
     2281 actcatcgga tataaatgac cgctaataac tgcgcgagcc atttcacccg ccaagactgg
     2341 agaaatattc tcacttttac ctaatgaagc ggtttgtaat aatagtcgcc aaatggaagg
     2401 tggtgttttc catgggcaag gctcaagagc gagatcttgc caatgctcgc tgaggttttt
     2461 tgccagttgc ccaaaggtgg tatccagcca aaaacgtacc gaaatgcgag ctgcatttgg
     2521 agcaagcccc aacacataaa aacgggtgtt ttcagataat tctggggaaa tttctgcaag
     2581 cggtcgtcct ttgctgattt tttgcaattc ttcaaagatt ttttggtttt cttgctcatc
     2641 atctggaggc gtcattaaag cagcaaaaaa gccttcagca gcttcagctt gggtggtatt
     2701 gtctgcttct gcccaaaaga cagtgctggc atcaccaata gttaaacgat gtttctcggt
     2761 gcgtaacaaa taatttaaag cagtagtgta ggcaaaggcg gcttgttcag aaacaggggc
     2821 attactacct tgttccttgc caaatgaagt gaaggattct ttattgaaag agatgattga
     2881 accaccggaa ctttgcccac caaatacccc tttaatagga ggatgagttc gttgaattgg
     2941 cgataatttg cctgaaatta agcataaacc tttttcagca ttatcacttt gatttatcaa
     3001 caaacttgtc caaagggttt gtgcagcttc tcgctgatga atataaccgc ttgtgccgac
     3061 taatttaaac acgacattcg catcaagaat ttctcttggg cagattgatt cggcaaaatg
     3121 tgtgggttgc cattgttgca ggaaatttct tatcgccatt aaaccttcat cattggaatc
     3181 tgcaagcaaa tccaaatggt actgtttaaa tgcctcaaag gttttctcgg cgaaattgat
     3241 gggttgatct ttggcggtgg ctttatcagg attactttca atgcctagca cataagcggt
     3301 tttatcccac agaaaattgg gcttaatgcc agacgtgcgt ttttcagggc gaggtacgct
     3361 catcaatttc ggttttggtt tcttatcttc tgttaaattt gagatcacat tgaccaaatt
     3421 tccctttgaa tctaattcta gtacccaacc gattttttct tcactgaaac cataagaagg
     3481 cactttagct tcgcctgtta agcgatcttc ttgtgctgca aggcggtaat aatagcgagt
     3541 taaggcggta ataatagcga gttaaggcgg agagaatcat gatttgacct cctctgcata
     3601 aaatggtggc acatcaatca cgccattttt catttccgca cggaagaaac gaggttgatt
     3661 accgtgctca aaatcaatgt cgtgcaacat ccagcctaaa tcccgatctc gctcattttc
     3721 tgccaaaaaa cactcaggta acggttcatc gtcatcaatc aaggcaaaat ttgcggcaaa
     3781 ttcacgcacg cccatacaag gctgctggaa acattgcccc tttttcgcac ggcgtttaaa
     3841 catttcaata tgcttggtga cggtttccgc ctcgcctgcc attttggtca gcactgcgtg
     3901 agcttcgatc acataaccga catttttcag cacagtagca gcacgttgtt ggcggtcgtc
     3961 ttcaatcaca gtatataaat ccgccactgt tttacgcttc attgcaccac tgattttact
     4021 ggcagaaatt ttactgccta actcattacg gcgaacagac tcaaattgaa tcggttgtaa
     4081 tacataaatt ttatcaatca cccagcgaat tgccggcttc caatgcaccg cagccaaaat
     4141 cccccgtgcc gcagacggcg taatcacatc ataagacacc cgttctacct tcatttcagg
     4201 acgggtaaaa caagcataat caccccaaat atggagcctg attttgtttg gcataataga
     4261 atgctccttt taattatgta acacacacct ccccgaaggg aggtgaaatt tctttagctt
     4321 atattataac tgctttggtt tcaacccacg cactcagtaa aatgcgaaaa attttatggc
     4381 ttattatatt aagcggtcgg atttacttag aaagttgcaa agttatttga gtatttaacc
     4441 gcttgtattt caaaaatagt cagaaaaata acagcattct aggtacaata tttttctaag
     4501 aacgaacttg tctccgcaat gcctctactg cgtttgacac agcatttggt ttgggctcct
     4561 ggtttgccca atcagaccag aattggcgtg tagcactttc tggggctata tgcggtttag
     4621 caagtttgat tcgtgtaggt aatagaatcg aatcgcctag caataacgct tcgcctaaat
     4681 caagtaacgg caatatatca gtaattccaa ccatagattc cggcaataaa cgctttacaa
     4741 cactttgatc ttgaccattt gttaatcgta aaaccaagaa attattacat tgactcaaaa
     4801 ttgttcggct tacatctgaa ggacgttggc taacgaccaa caaagacacg ccatattttc
     4861 taccttcttt cgcaatccgc tcaaaggctt ccaatgcgcg tttttccgct gcttccgctg
     4921 aatctcgaac agggagatat aaatgtgctt catcgcaaat aatcgttagt ggatggcggg
     4981 aatctttatc catccaaaat tgaatgttgt ataaaaggcg ggcaagaact cccgtaacaa
     5041 tcggtaaaac atccgacggg acttccgaaa aatcaataat ttttatccct ttttcgctct
     5101 cagaagcaga aaacagtttt gacaacatat tttccagcca cccatagctg ttgcactcta
     5161 ctggcggttg gaacataaaa ccgcaacgtc tgtcatctat tttggctttc atccttccca
     5221 taaacctaga caacttacta taccaaggac cttgctttgt tcctgaacct ccctctttag
     5281 gtaccatctc agaattatct ttatctaaac gagctaacaa taattcaata gaatacggaa
     5341 ttggcgaatc aacagtaaac gtttccaaaa catcatttct ttcttcctta ttcaatgttt
     5401 gctcttttaa tgcgcgaaca tgctcagtaa aacgcgccgc ctgattcgga gcattatcat
     5461 cactgcgatc taaaattaaa gccagcattt attcccgatt gagcagccaa tagggaataa
     5521 ataaactgcg ttcatctgaa ccatccaaat ctcccggtcc agcaatacgg tattgctgag
     5581 cgatagcgtt tttaccttcg catagagtgc tatattcacc gtgcatatcg aatacaataa
     5641 gattagcagc ttttaaagct gctgcacgtt ccaatattaa tgcaactgac cagcttttac
     5701 cggaaccagt actacccaaa atagcagcgt gccgttgaaa gaatttatct ccgtttgcta
     5761 tagctacagc cgtttcatca gccataaaat gacctaaagt cagccgttca ttttctttta
     5821 cgttagcgga caaaagattc ataaataatt gcagattgct gccactaata tgaaagcaat
     5881 ctctatcaat ttgcggaaaa caatccgctc cccgcttaaa agtgttgctc aaactaccat
     5941 caacagcttt ataagtaccg attaatactg ccttgataaa gtcatcctga ctttcaccca
     6001 gaggaatttc accctcatta gatatttctt ctagcagcaa tttttcactg atagaacgcg
     6061 ttacccgttc gataatgccg ataaggtact cactagccgt tctgccttga atcgcaatca
     6121 gattacccac agcaatacta gtcatttgtt gatgctcttc aacagaaatc aatacacggc
     6181 tggtatctac tgttacaacc ctgcccaaac gggcattgtc tttaaaattc aatgtttcat
     6241 ttgccattct ctaaaatctc ttttacaaaa aactcaacgt cccaccaatg aacattagga
     6301 ataaaagttt cttcttgctt gctaaccacc cttgttccag ccttatctcc atcaaaaatc
     6361 agagccttac aattcggagc ttccttcacc acgtttctgg cattttctga aagtcctctt
     6421 gtcagaagta gtgttttcac accactttta attttgcctg tcaaatgtgt ttgaaggtgg
     6481 tcatcattaa aaccatagcc gatacataaa attgaagaag cagaatcaat agcttcattt
     6541 gctttttctc gatgagcatc aaatggttgt ccatagcctt ttttatactt cccaacacca
     6601 ggcgtaataa ttaaaggctc ttccacttta cctatacaac ttgtcattgg aacgtctcca
     6661 gccataaacc aatctaaact accatgcggt ttaaagattt tgatatgatc tc
//
LOCUS       DANLXS010000132         6711 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.133, whole genome shotgun sequence.
ACCESSION   DANLXS010000132 DANLXS010000000
VERSION     DANLXS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.133"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..4452)
                     /gene="mukB"
                     /locus_tag="PXD71_001961"
     CDS             complement(<1..4452)
                     /gene="mukB"
                     /locus_tag="PXD71_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDL6292045.1"
                     /translation="MIDTTELQNNPQIAPLARPNDVQRGKFLSLTLINWNGFFARTFD
                     LDDLVTTLSGGNGAGKSTTMAGFVTALIPDLTLLNFRNTTEAGSTAGSRDKGLYGKLK
                     AGVCYAVLESFNSRGQRIITGVRLQQIVGRDKKVDIRSFSLQNIPQEQSIISLLTEQV
                     GNKARVLALNELKDQFENSEVQFKQYHSITDYHSFMFDLGVIPKRLRSAADRSKFYKL
                     IEASLYGGISSVITKSLRDYLLPENTGVRQAFQDMESALRENRMTLEAIKVTQSDRDM
                     FKRLITESTNYVSADYMRNANERRGNVQQALEQRKEWYAAKSKILLEQQRFVEFSRES
                     ADIAEAELALEADYNSANDHLNLVMNALRHQEKIERYQDEVEELNIKLEEQQEALEEI
                     AEIAENAQARADEADDHVEELRSQMADYQQALDAQQTRALQYQQAVNALEKAKQLTGL
                     VNLDLNNIEDYHAEFVAQAEDLTDQVFELEQRLSVSDMAKTQFEKAFELVCKISGEID
                     RLQAWEEARALLSAFPEQKMQAQQAVSLRQKLNDLEQRLQQQQNAQRLVAEFNQKSQQ
                     QLNSAEELDAYFEEQQARLEDLEAELSDFVEQRSTQRQQREQLNQQYQQLAKNAPAWH
                     TAQSALTRLEEQCGEKFEASQSVMQFMQNMLSKEREATLARDELARKEQMLDEQINRL
                     SQPDGSEDARLNQLAERFGGVLLSELYDDVSIEDAPYFSALYGEARHAIVVRDLEAVK
                     TQLEKLDDCPSDLYLIEGDPSAFDDAVFSAEELGEGVVVKVSDRQWRYSKFPEVPLFG
                     RAAREKHLATLKAERDETAEKHAERAFDVQKCQRLHQHLSQFIGTHLNLAFQENPEML
                     MQEIAAERAEIERELAQSAGNEQQLRHQLESVKAQLQMLNKILPLANLLADETLEARA
                     EECREQLELAEEDEMFIRQFGATLSQLEPIAAALKSDPALFEQLEADYRRSVAEQKVL
                     QQKVFSLADVMNRRLHFSYQETVGTEGSALTEQLRQRLENAQREREQARDQLRQAQAQ
                     LSQYNQVLTGLRSSYEAKNQMLQELIREIDDLGVRGDSGAEERARFRRDELQQRLSQQ
                     RSRKGYLDKQIAVIEAEMDNLNRTLRKAERDYHTQRELVVQAKASWCIVQRLSRNSDV
                     EKRLNRRELAYQSAEELRSISDKALGALRTAVADNEYLRDSLRASEDSRKPENKVAFF
                     IAVYQHLRERIRQDIIKTDDPIDAIEQMEIELSRLTNELTGREKKLAISAESVANILR
                     KTIQREQQRILQLNQGLQNIAFGQVKGVRLVVNIRDTHAILLNALSNNREEHKDLFDS
                     QKLSFSEALAMLYKRVNPHIEMGQRTPQTIGEELLDYRNYLDLEVETFRGADGWMRAE
                     SSALSTGEAIGTGMSILLMVVQSWEEEARRMRAKDILPARLLFLDEAARLDATSINTL
                     FELCERLDMQLLIAAPENISPERGTTYKLVRKITNNQEYVHVVGLKGF"
     gene            complement(4628..5344)
                     /gene="mukE"
                     /locus_tag="PXD71_001962"
     CDS             complement(4628..5344)
                     /gene="mukE"
                     /locus_tag="PXD71_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDL6292046.1"
                     /translation="MTENIQDFISPKLALAIANPIFPELDSQLRAGRHINTEQLDQHV
                     FLMDFQAELESFYRRYHVELIRAPEGFFYLRPKASTLIARSAMSEMEMLVGKVLCYLY
                     LSPERLAQQGIFSQDDVYEELLSLADESKLLKAVNPRSTGSDLDRAKLAEKAGGALRR
                     LARIGIITRVGEQNSKKFVISESVFRFGADVRTGDDPREAQLRLIRDGEATTPALLNE
                     QAVEIDENIADEAPFDGENE"
     gene            complement(5393..>6711)
                     /gene="mukF"
                     /locus_tag="PXD71_001963"
     CDS             complement(5393..>6711)
                     /gene="mukF"
                     /locus_tag="PXD71_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDL6292047.1"
                     /translation="PELISWTKDREFSLSLSSDRLAFLLAISIYNQEQTDGELLESDL
                     IDLFRYVSNSFEQSDATFTQRANNAINDLVRQRLLNRFSSEFTEGLAIYRVTPLGVGI
                     SNYYVRQREFSTLRLSIQLSIVADEIQRASVAAEEGIEQAQDERFWRNNVFAPLKYSV
                     AEIFDSIDLSQRIMDENQHQIRERIAELLSQNWHEAIMSCEQLLDETSGNLRELQDTL
                     NAAGDKLQAQLLRIQSCLMARNDLDFIDQLIVNLQNKLDRIISWGQQAIDLWIGYDRH
                     VHKFIRTAIDMDKNRVFGQRLRQSIQDYYNSPWLLYTAKADSLLDLRDDEAMLNEAES
                     VGELPSELEYESLSDVQEQIVSAMQAHLAPFRAEGKPIDLGVILREQLARFPESRHFD
                     VARIIVDQAVKLGMASQDSQAVYPQWQPINEQGAEVQANVIDQYNK"
BASE COUNT         1718 a         1625 c         1378 g         1990 t
ORIGIN      
        1 aaagcctttt aaaccaacaa cgtgaacata ctcttggtta ttggtgattt tacgcaccaa
       61 tttataagtt gtgcctcgtt cagggctaat attttcaggg gctgcaatta ataactgcat
      121 atccaaacgt tcgcagagtt caaatagcgt attaattgag gtagcatcta atcgagccgc
      181 ttcatctaag aacaataagc gagccggtag aatatctttg gcacgcatac ggcgagcttc
      241 ttcttcccag ctttgaacta ccattaagag aatcgacata cctgtaccga tcgcctcacc
      301 ggtcgataat gcactacttt ctgcacgcat ccagccgtct gcaccacgga aggtttccac
      361 ttctaaatct aagtagttac ggtaatccaa taactcttca ccaatggtct gtggtgtacg
      421 ttgccccatc tcaatatgag ggttcacccg tttatacaac atcgctaagg cttcggagaa
      481 gctcagtttt tggctgtcga ataaatcttt gtgttcctca cgattattgg ataacgcatt
      541 gagtaaaata gcgtgcgtat ctcggatatt caccaccaaa cgcacccctt ttacctgacc
      601 gaatgcaata ttttgtaaac cttggttcag ttgcaaaatg cgttgttgtt cacgctgaat
      661 ggttttacgt aaaatatttg ccacactttc cgcactgatc gccagttttt tctcacgacc
      721 ggttaattca ttggttaagc gtgaaagctc gatttccatc tgctcaatcg catcaatcgg
      781 atcatcggtt ttgataatgt cttggcgaat ccgctcacgc aaatgctgat aaaccgcaat
      841 aaagaacgcc actttgtttt ccggcttgcg actatcttca gaagcccgca agctatcacg
      901 caggtattca ttatccgcca cggcggtgcg taatgccccg agagctttat ccgaaatcga
      961 acgtaattct tcagccgatt gataagccaa ttcacggcga tttaaacgtt tttccacatc
     1021 gctgttacga gataaacgtt gcacaataca ccagctggct tttgcctgca ccaccaattc
     1081 acgttgtgta tgataatcac gttcggcttt acgtaaggtg cggttaaggt tatccatttc
     1141 cgcctcgatc accgcaattt gtttatctaa ataacctttg cgggaacgtt gctggcttaa
     1201 acgttgttgc aattcatcac ggcggaatct ggctcgctct tccgctccgc tatcaccacg
     1261 cacgcccaaa tcgtcaattt cacgaattaa ctcttgtagc atttggtttt tcgcctcata
     1321 agaactgcgt aagccggtca atacttggtt atattgtgaa agttgagctt gagcttgacg
     1381 cagctgatca cgagcctgtt cacgctcacg ttgtgcattt tccaaacgtt ggcgtagctg
     1441 ttcggttaat gcagagcctt ctgtacccac cgtttcttga tagctaaaat ggaggcggcg
     1501 attcattaca tcagccaagc tgaacacttt ttgctgtaac actttttgct cggcaactga
     1561 acggcggtaa tcggcttcca actgttcaaa taaagccgga tcacttttta atgcggctgc
     1621 aatcggctca agttggctta atgttgctcc aaattggcgg ataaacattt catcttcttc
     1681 tgccaattct aattgctcac gacattcctc cgctcgtgct tccagcgttt catcggctaa
     1741 taaattcgca agcggtagaa ttttattcaa catttgcaac tgggctttta cgctttctaa
     1801 ttggtgacgt aattgctgtt cgttaccggc tgattgtgcc aattcacgtt caatttctgc
     1861 ccgttctgcc gcaatctctt gcattaacat ttcagggttt tcttggaacg ctaaatttaa
     1921 gtgcgtgccg ataaattggc ttaagtgttg atgtaaacgc tggcatttct gcacatcaaa
     1981 ggcacgttca gcgtgttttt ctgcggtttc atcacgctct gcttttaaag tcgctaaatg
     2041 tttttcacga gcagcacgtc cgaataacgg cacttcaggg aatttagaat aacgccattg
     2101 gcgatcggag actttcacca ccacgccttc gcctaattcc tcggcactaa ataccgcatc
     2161 gtcaaaggcg gacggatcac cttcaattaa ataaagatca cttgggcaat cgtctaattt
     2221 ctctagctgt gttttaaccg cctctaaatc acgcactaca atagcgtggc gagcctcgcc
     2281 gtataaggcg gagaaataag gcgcatcttc aattgataca tcatcatata actcagagag
     2341 taacactcca ccaaaacgtt ctgccaactg gtttaagcgg gcgtcttccg agccgtccgg
     2401 ctggcttaag cgattaattt gctcatcaag catttgttct ttacgagcca attcatcacg
     2461 agcaagggtg gcttcacgct ctttgctcag catattttgc ataaactgca tcactgattg
     2521 gctggcttca aacttctcgc cacattgctc ttctaaacga gtcagagccg attgtgccgt
     2581 atgccaagcc ggtgcattct tcgctaattg ctgatattgt tgattaagct gttcacgttg
     2641 ttggcgttgg gttgaacgtt gttctacaaa gtccgaaagc tctgcctcta aatcttctag
     2701 gcgagcttgc tgctcttcaa aataggcgtc taattcttcc gctgaattga gttgttgttg
     2761 cgatttttgg ttaaattccg ccactaaacg ctgggcattt tgctgttgtt gcaaacgctg
     2821 ttctaaatcg ttgagttttt gacgtaaact caccgcttgc tgagcttgca ttttttgctc
     2881 agggaaggca gaaagtaacg cacgagcctc ttcccacgct tgcaagcggt caatttcacc
     2941 ggaaattttg caaaccaatt caaacgcttt ttcaaattgg gttttcgcca tatccgacac
     3001 gcttaaacgt tgttccagct caaatacttg atcggtaaga tcttcggctt gagccacaaa
     3061 ttctgcgtgg taatcttcaa tattatttaa atctaaattc actaagccgg ttagctgttt
     3121 ggctttttct aacgcattca ccgcttgttg gtattgcaat gcacgagttt gctgtgcatc
     3181 taaggcttgt tggtaatccg ccatttgaga acgcaattct tcaacgtgat catctgcctc
     3241 atccgcacga gcctgagcat tttcggcaat ttccgcaatt tcttccaatg cctcttgctg
     3301 ttcttccagc ttaatattca gctcttcaac ttcgtcttgg tagcgttcga ttttctcttg
     3361 gtggcgtaac gcattcatca ctaaatttag atgatcgttc gcactattgt aatccgcctc
     3421 taacgccagc tctgcttcgg caatatccgc actttcacgg ctaaattcca caaagcgttg
     3481 ctgttcgagc aaaattttgg attttgccgc ataccactct ttgcgttgct ctaaggcttg
     3541 ttgtacattg ccacggcgtt cattcgcatt acgcatataa tccgccgaca cataattggt
     3601 cgattcggta attaagcgtt tgaacatatc ccgatccgat tgcgtgacct taatcgcctc
     3661 taaggtcata cggttttcac gcagagcgga ttccatatct tggaacgctt ggcgaacgcc
     3721 cgtattttcc ggcaacaagt aatcacgcaa ggatttggta atcacacttg aaataccacc
     3781 ataaagcgag gcttcgatta atttgtagaa tttgctacgg tcggctgccg aacgaaggcg
     3841 ttttgggatt acgcccaaat cgaacataaa actgtggtaa tcagtaatcg aatggtactg
     3901 tttgaattgg acttcgctgt tctcaaattg atctttgagt tcatttaacg ccagcactcg
     3961 agccttattc cccacttgtt cggtcagtag gctgataatg ctttgctctt gaggaatatt
     4021 ctggagcgag aacgaacgaa tatcgacttt tttatcccgt ccgacaattt gttgcaaacg
     4081 cacgccggta ataatccgtt gcccacggga attgaatgac tctaacaccg catagcaaac
     4141 acccgctttt aacttaccgt ataagccttt atcacgagaa ccggcggttg agcctgcttc
     4201 agtcgtatta cggaaattaa gcaaggttaa atctgggatc aaggcggtca caaaccctgc
     4261 cattgtggtg gacttacctg ccccgttacc gcctgaaagc gtggtcacta agtcatctaa
     4321 atcaaaggtt cgagcaaaaa aaccgttcca gttaatcagc gttaaagaga gaaatttacc
     4381 gcgttgtaca tcattcgggc gtgcaagcgg tgcaatttga ggattatttt gcaattctgt
     4441 ggtatcaatc attatgtttt ctcactaatt gtcctagcac gctaaagcgt actaggttag
     4501 gtataggtga gtagctccgc tactctaatc gcaacctcag ccttggaggg ctgctttatg
     4561 cttaacctag ttcggcttta ccgtgtctag ggcagactca ttaaatgctg aatctattcg
     4621 aatgaaatca ctcattttct ccgtcaaagg gggcttcatc tgcaatattt tcatctattt
     4681 caaccgcttg ttcgtttaac aacgcagggg ttgtcgcttc accatcacga attaagcgaa
     4741 gctgtgcctc tcttgggtca tcaccggtac gcacatcagc cccaaaacgg aagaccgatt
     4801 ctgaaatcac aaattttttg ctgttttgct cgcctactcg ggtaataatg ccgatacggg
     4861 ctaatctgcg taacgctccg ccggcttttt ctgccagctt ggcacggtct aaatcggaac
     4921 cggtcgaacg tgggtttacc gcttttaata atttgctttc atcggcaaga ctgagtaatt
     4981 cttcatacac atcatcttgg ctgaaaatcc cctgctgagc caaacgttcc ggacttaaat
     5041 agaggtagca caacacctta cccactaaca tttccatttc tgacattgcc gaacgggcaa
     5101 ttagtgtcga ggctttcggg cgtaggtaga aaaagccttc cggtgctcga atcaactcaa
     5161 cgtgataacg gcggtaaaaa ctctctagtt ccgcttgaaa atccatcaaa aagacgtgtt
     5221 ggtcaagctg ctcggtattg atatggcgac cggctcgtaa ttggctgtct agctccggaa
     5281 aaatcgggtt ggcaatcgca agtgccagtt tcggggaaat gaaatcttgg atattctctg
     5341 tcattgtcat tctcttcaat tttgcaaaat tctgggtaaa aacgaccgct tgttatttat
     5401 tgtattggtc gataacattc gcctgcactt ccgccccttg ctcgttaatc ggctgccatt
     5461 gtgggtaaac ggcttggcta tcttggcttg ccatacctaa cttaacagct tgatcgacaa
     5521 taatacgagc cacgtcaaaa tggcgggatt ccgggaatcg ggcaagctgt tcacgcaaga
     5581 taacgcctaa atctatcggt ttaccttcag cacggaacgg cgctaaatga gcttgcattg
     5641 ccgaaacaat ttgctcttgc acatctgaaa gcgactcata ctctaactca cttggcaact
     5701 cgcccaccga ttctgcctca tttaacattg cttcatcatc acgcaagtcc agtaacgaat
     5761 cggcttttgc agtataaagc aaccaagggc tgttgtaata atcttgaatc gattggcgta
     5821 accgttgccc gaatacacgg tttttgtcca tatcaatcgc tgtacgaata aatttatgca
     5881 catggcggtc atagccgatc caaagatcga tagcttgttg tccccagctg ataatacggt
     5941 ctaacttatt ttgtagattc acaatcagct gatcgataaa atccaaatca ttacgagcca
     6001 tcaggcaact ttgaatccgc aataattgag cttgcagttt atcaccagcg gcatttaagg
     6061 tatcttgcaa ctcacgcaaa ttccccgagg tttcgtctaa caattgctca caactcataa
     6121 ttgcttcgtg ccagttttgg ctaagcagtt cggctattcg ttcacggatt tggtgctgat
     6181 tttcgtccat aatccgttgt gaaagatcga tgctgtcaaa gatttccgcc accgaatatt
     6241 tcaatggggc aaacacatta tttcgccaaa aacgttcatc ttgtgcctgc tctattccct
     6301 cttccgctgc tactgaagct cgctgaattt catctgccac aatcgaaagt tgaatcgata
     6361 aacgcagggt agaaaattcc cgctggcgaa cgtaataatt tgaaattccc acccctaacg
     6421 gcgtaacacg ataaatcgcc aaaccttccg taaattcgct actaaaacgg tttaacaacc
     6481 gttgtctgac taaatcatta atcgcattat tggcacgctg ggtaaaagtg gcatcagatt
     6541 gctcaaacga atttgaaaca taacggaaaa gatcaataag atcactttct aacaattcac
     6601 catctgtctg ttcttggtta taaatagaga tagcaagtag aaacgctaag cggtcggaag
     6661 aaagcgaaag tgaaaattca cgatctttcg tccaagagat gagttctgga a
//
LOCUS       DANLXS010000133         6691 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.134, whole genome shotgun sequence.
ACCESSION   DANLXS010000133 DANLXS010000000
VERSION     DANLXS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.134"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(56..3085)
                     /locus_tag="PXD71_001964"
     CDS             complement(56..3085)
                     /locus_tag="PXD71_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HDL6292048.1"
                     /translation="MSLPNYYQDPSVLHLNTTLHHAYFIPFAKGQTPNQLEREQSELF
                     TLLNGDWKFNFYPSPHDLPEDFLNTKFTHQIPVPSNWQTQGFDAHQYTNINYPIPFDP
                     PYVPHQNPCGVYQREITFNPKPNKRYLLNFEGVDSCLFVYVNGEFVGYGQISHSTNEF
                     DITKQLKQGKNSLVVVVLKWCDGSYLEDQDKFRMSGIFRDVYILEREQNYLQDFFIRY
                     QLNADLSKAELKVETQFSTYTEAVHFQLFDPENRLVCEQTSGDFCQEICNLCLWNAED
                     PTLYTLCLSYQNEIIEQKIGFRQIRVENGVMLFNNAPIKFKGVNRHDSDPKTGYSISR
                     EQALVDLRLMKAHNINAIRTAHYPNAPWFSELCDQLGFYLIAESDLESHGSSAVYIES
                     PENSIFLNVPNPNKQEEIRQQAVDNFCYFARDPLFKQAILDRNIANVERDKNRTSVII
                     WSLGNESGFGENFEVAARWVKQRDPSRLTHYEGSIYQHSAHQNDLSNLDLYSEMYPST
                     ERLEEYFSDSANHKPFILCEYSHAMGNSSGDMEDYWQTFQRYSGACGGFVWEWCDHAP
                     LLPNSELSAEQKTEKYGYGGDFGETLHDGNFCMDGLVSQQRVPHSNLLEVKNVNRPVR
                     AELKAGKIWLKNQLDFSDLADYLTVHYCFSENGETTLQGQCLVNCQPNQTACLPLTLP
                     EPNGKLQLLTLDYSLNESTALLEKGHHLGFDQITLHQAMVTPALNPRPQAVIFVQDFA
                     NEWRIQQGDYRYTFDKLKGIFSRIEKNGEAVIEQPLDFNIWRAPTDNDRLIREFWKKA
                     GYDNCYTRAYHTELMQDKNGVNITAECGIVATAQSRILTLKVNYHLAPNGEMTIQVQA
                     TRPAHLPFLPRFGLRFFLNKRNNQVSYFGYGEQESYIDKYHLAKLGAYQTSARENHTD
                     YLKPQENGSHFGTHYVQANGITVTSPQPFSFNLSPYTQEELTTKSHSYDLVESPSTVL
                     CVDYKMSGIGSNSCGPNLKPQYRLNETNFEWLLKLSF"
     gene            3263..4120
                     /gene="menB"
                     /locus_tag="PXD71_001965"
     CDS             3263..4120
                     /gene="menB"
                     /locus_tag="PXD71_001965"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDL6292049.1"
                     /translation="MLYPSEEFLYAPVEWVDHSEGYTDIRYHKSKDGIAKITINRPEV
                     RNAFRPQTVKEMIEAFSDARFDEKIGVIVLTGEGEYAFCAGGDQKVRGDYGGYKDESG
                     VHHLNVLDFQRDIRTCPKPVVAMVAGYAIGGGHVLHMLCDLTIAADNAKFGQTGPKVG
                     SFDGGWGASYMARLVGQKKAREIWFLCRQYNAQEALDMGLINTVVPYADLEKETVRWC
                     REMLQNSPIALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNEKREP
                     DFSKFRRNP"
     gene            4203..6605
                     /gene="mrcB"
                     /locus_tag="PXD71_001966"
     CDS             4203..6605
                     /gene="mrcB"
                     /locus_tag="PXD71_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HDL6292050.1"
                     /translation="MSDVELNEQSEQEPEQKPKKRNYFLSTLFKIGVIGACSMAFYGI
                     YLDGKIRSKMDGQIWELPAEVYARIESIKIEDNLTLEQTKQALLDNGYRQVSQVATPG
                     DFKIEENQLVLLRRAFPFPETPESQRVLRLTFKQDKLTYIEDLVQGKLIEEFRLDPKL
                     LALLHSDSDEERQALRLQQYPRLLIESLILTEDRRFYQHDGISPIGIARALITNYQAG
                     RTVQGGSTLTQQLVKNLFLSNERSLVRKVNEALMSVILDFRYDKNRILETYLNEIYLG
                     QNGSYQIHGFALASQFYFGRPIQEISLDQIALLVGMVKGPSLYNPWRNPQGAVERRNV
                     VLRLLLDHQVINQELYALLSSRPLNIREKGTIYREQPAFMQALTIDLKNELGEIKSAQ
                     LSGTKIFTTLDRKQQRSAEQAVITGLEDLESKNKKVSDLQSAIVVAEYRTGKVRAIVG
                     DRLTQFAGFNRAIQSKRQIGSLVKPSVYAIALAQPELFRLNTPIQNKQISIKVGSTYW
                     TPRNYDRSFSGSVMLMDALVRSLNIPTVNIGMKVGLKKVIAKQKEMGWDNAEIPPYPS
                     TLLGSYSISPYDVTRSYQVIANEGLKTPLTTIEAIISHNGEMFYQRDSGKVSEQVLPP
                     EAAVQTMYAMQQVVERGTARSLQNDFAKLKLAGKTGTTNNARDTWFVGIDGEHVTTVW
                     LGKDNNGDTNLTGSSGALYVYKQYLERALPQAYRLPKLNTMQWVGINSYGSWNCDSGR
                     QIPVWRDKGQRYCTSAGNVVATEKPSVWDALSLKKENIEAKAEEAEGKQPIPTEEVAP
                     IE"
BASE COUNT         1919 a         1384 c         1551 g         1837 t
ORIGIN      
        1 tatgctgaaa taaaaggaga tgctcgttca attaaatttg aacgagcatt tttcgttaaa
       61 aactcaattt aagcagccac tcaaaattag tctcatttaa ccgatattgc ggtttcaaat
      121 tcgggccgca agagtttgag ccaatgccgc tcattttgta atcgacacac agcacggtgg
      181 aagggctttc taccaaatca taactgtggc ttttagtggt tagctcttcc tgtgtgtaag
      241 gcgaaagatt aaaactaaac ggctgtggag aagtaacagt tatcccattt gcttgcacat
      301 aatgcgtacc gaaatggctg ccgttttcct gcggtttgag ataatcggta tgattttctc
      361 gggcgctggt ttgataagct ccgagtttcg ccaaatggta tttgtcgata taactctcct
      421 gctcgccata gccaaaatag ctcacttggt tattccgttt attcaagaaa aaccgcaagc
      481 caaagcgagg taaaaacggc aaatgagcgg ggcgggttgc ctgaacctga atcgtcattt
      541 cgccattcgg ggcaagatga taattcactt tcagggttaa aatccgactt tgagcggttg
      601 ccacaatgcc acattcggca gtaatgttta cgccgttttt atcttgcatc agttcggtat
      661 gatacgccct ggtgtaacag ttatcgtagc cggctttttt ccaaaactca cgaattaagc
      721 ggtcgttatc ggttggggct cgccaaatat tgaaatccaa cggttgctca attactgcct
      781 cgccgttttt ctcaatccgg ctgaaaatgc ctttgagttt atcgaaagtg tagcgatagt
      841 cgccctgttg aatccgccat tcatttgcaa aatcttgcac aaaaatgacc gcttgcgggc
      901 gtggattaag tgctggagtg accatcgctt gatgcagcgt gatttggtca aagcctaagt
      961 gatgcccttt ttccaacaga gccgtgcttt catttaaaga ataatcaagg gttaaaagtt
     1021 gcaatttgcc gtttggttcg ggcagcgtta gcggcaaaca agcggtctga tttggctgac
     1081 aatttaccaa acattgtccc tgcagggtgg tttcgccgtt ttcgctgaaa cagtaatgca
     1141 cggtcagata atcagctaag tcgctgaaat ccaactgatt tttcagccaa attttgcccg
     1201 cttttaactc agctcgcacc gggcgattca catttttcac ttctaacaaa ttagagtgtg
     1261 gcacacgctg ttgggaaacc aagccgtcca tacagaaatt gccatcgtgc aaggtttcgc
     1321 cgaaatcacc gccatagccg tatttttcgg tcttctgttc ggcacttagc tcagagttgg
     1381 ggagcagagg agcatggtcg caccattccc acacaaagcc accgcacgca ccgctgtagc
     1441 gttggaaggt ttgccaataa tcttccatat caccactcga attgcccatc gcgtgagaat
     1501 attcacacaa aataaaaggt ttgtggttgg cagagtcgga aaaatattcc tctaaccgct
     1561 cggtgcttgg gtacatctcg ctgtataaat cgaggtttga aagatcgttt tgatgtgccg
     1621 aatgttggta aatcgaacct tcgtagtgag ttaaacggct tggatcccgt tgttttaccc
     1681 aacgggctgc gacttcaaaa ttttcaccaa agccggattc gttaccaagt gaccaaataa
     1741 tgaccgaagt gcggtttttg tctcgctcca cattggcaat gttgcgatct aaaatcgcct
     1801 gtttgaacag cggatctctt gcaaaataac agaaattatc gaccgcttgt tggcgaattt
     1861 cttcctgttt gttcgggttt ggcacattca ggaaaatgct gttttccgga ctttcaatat
     1921 acactgcact ggagccgtga gattcaagat cactttcggc aatcagataa aaaccgagct
     1981 gatcgcacaa ttcactaaac cacggagcgt tcgggtaatg ggcggtgcga atagcgttga
     2041 tgttatgggc tttcattaag cgtaaatcca ccaacgcttg ctcacggcta atgctgtagc
     2101 cggttttcgg gtcggaatcg tggcggttta cccctttaaa tttaatcggg gcgttgttga
     2161 atagcatcac gccattttcc acacgaatct gacgaaagcc gattttttgc tcaataattt
     2221 cgttttggta gctcaagcat agcgtgtaga gtgtcggatc ttccgcattc cacaaacaca
     2281 aattgcaaat ttcttggcaa aaatcaccgc ttgtttgctc gcagactaag cggttttcag
     2341 ggtcgaaaag ttgaaaatgc acagcttcgg tgtaggtgga aaattgtgtt tccaccttga
     2401 gttctgcctt tgaaagatca gcgtttaact ggtagcggat aaagaaatct tgcaggtagt
     2461 tttgctcacg ttccaaaata taaacatcac ggaaaatgcc tgacattcgg aatttgtctt
     2521 gatcttccaa ataactgccg tcacaccatt ttagcaccac cacgacgagg ctgtttttgc
     2581 cctgtttaag ctgtttggtg atgtcaaatt cattggtgct gtggctgatt tgcccgtagc
     2641 cgacaaattc gccgtttaca taaacaaaca ggcacgaatc tacgccctca aaatttagca
     2701 aatagcgttt gttaggcttg gggttaaacg tgatttcacg ctgatacacc ccgcaagggt
     2761 tttggtgtgg cacataaggc ggatcaaacg gaatcgggta gttgatgtta gtgtattgat
     2821 gggcatcaaa gccttgagtt tgccagttag acggcacggg gatttggtgg gtaaatttgg
     2881 tgttgaggaa atcctccggc agatcgtgcg gacttggata gaaattaaac ttccaatcgc
     2941 catttagcaa agtaaataac tcagattgtt cccgttccaa ctgattcggc gtttgccctt
     3001 tagcaaaagg aataaaataa gcgtggtgta gcgtggtatt aaggtgcaac acactcgggt
     3061 cttggtagta attcggtaac gacataaaag ccctatgatc aaaaagatat aaatacgaat
     3121 acactttaat tctcttataa aatagggcaa tcaactatgt gaaaaattgt gaggcggatc
     3181 acaaaataat ttcctcacct ttttccttcg attccgttat aatcacaaga attttttatg
     3241 tcatttttca aggaatttac caatgttata tccaagcgaa gaatttttat atgcaccggt
     3301 tgagtgggtt gatcacagcg aaggttacac cgatattcgt taccataaat caaaagacgg
     3361 tatcgctaaa atcaccatta atcgtccgga agtgcgtaat gctttccgtc cgcaaacggt
     3421 aaaagagatg attgaagcct tttctgatgc ccgttttgat gaaaaaatcg gcgtcatcgt
     3481 attaaccggt gaaggcgaat atgcgttctg tgccggcggc gaccaaaaag tgcgtggcga
     3541 ttacggcggc tacaaagatg aaagcggtgt gcatcactta aatgtgttgg atttccaacg
     3601 tgatattcgt acctgcccga aaccggtggt ggcaatggtt gccggctatg cgattggtgg
     3661 tggacacgtt ttacatatgt tatgtgattt aaccattgcc gctgacaatg caaaattcgg
     3721 tcaaaccggc ccgaaagtgg ggtcatttga cggcggttgg ggtgcaagct atatggcacg
     3781 tttagtcggt cagaaaaaag cgcgtgaaat ttggttctta tgccgtcaat ataatgcaca
     3841 agaagcgtta gatatgggct taatcaatac cgttgtgcct tatgctgatt tagaaaaaga
     3901 gactgtgcgt tggtgtcgcg aaatgctaca aaacagcccg attgccttac gttgcttaaa
     3961 agcagcattg aatgcagatt gtgacggtca ggctggcctg caagagcttg ctggtaatgc
     4021 aacaatgttg ttctatatga cagaagaagg gcaagaagga cgcaatgcgt tcaacgagaa
     4081 acgtgagccg gacttcagca aattccgccg caatccataa ttgacggcaa atacaagcgg
     4141 ttcgattttg caaaaaattt gcaaaaatcg accgcttacc ttttctaatt taagttaatt
     4201 ttatgtcaga tgttgagtta aacgagcaga gcgagcaaga gcctgagcaa aaaccgaaaa
     4261 agcgaaacta tttcctttca accttattca aaatcggggt gataggagct tgcagtatgg
     4321 ctttttacgg catttattta gacggtaaaa tccgtagcaa aatggatggg cagatatggg
     4381 aattaccagc tgaggtgtat gctcgtattg aaagcattaa aattgaagac aacttaacgc
     4441 ttgagcaaac caaacaagcg ttgctggata acggctatcg tcaagtttct caagttgcga
     4501 caccaggcga tttcaaaatt gaagaaaatc agctggtgtt gctacgccga gctttcccgt
     4561 ttccagaaac accggaatcg caacgagtgc ttcgtttaac tttcaaacaa gataaattaa
     4621 cttatattga agatttggtg cagggtaagt tgatcgagga gttccgcttg gatccgaaat
     4681 tactggcgtt actgcattcc gatagcgatg aagaacgtca agcattacgc ttacaacaat
     4741 accctcgctt gttaattgag tcactgattt taaccgaaga tcgccgcttt tatcagcacg
     4801 atgggattag cccaatcggt attgcccgtg ctttaatcac taactaccaa gctggcagaa
     4861 cagtgcaggg tggcagtacc ttaacgcaac agttagtgaa aaacttgttc ttaagtaatg
     4921 aaagatcgtt ggtgcgtaag gtaaatgaag cgttaatgtc ggtgatttta gacttccgtt
     4981 acgataaaaa ccgcatttta gaaacctatc tcaacgaaat ttatttaggg cagaacggta
     5041 gctaccaaat tcacggtttt gcattggcaa gccaattcta ttttggtcgt ccgatccaag
     5101 agattagctt agaccaaatt gcgttattgg tgggaatggt aaaaggtcca tcactgtata
     5161 acccgtggcg aaatccgcaa ggtgcggtag agcgacgtaa tgtggtgttg cgattattgc
     5221 tggatcatca agtgatcaat caagaacttt acgctctgtt aagctcccgc ccgcttaata
     5281 ttcgtgaaaa aggcacgatt taccgtgagc aacctgcctt tatgcaggca ttaaccattg
     5341 atttgaaaaa tgagttaggc gaaattaagt cagctcaact ttccggtact aaaattttta
     5401 cgacccttga ccgtaaacag caacgttcag ccgaacaagc agtgattacc ggtttggaag
     5461 atctggaaag taaaaataaa aaagtcagcg atttgcaatc ggctattgtc gtggcggaat
     5521 accgcacagg taaagtgagg gcgatagtag gagaccgctt aacccaattt gccggcttta
     5581 accgtgcgat tcagtcaaaa cgccaaatcg gttctttagt caaaccatcg gtttatgcga
     5641 ttgccttggc acagcctgaa ttattccgtt taaatacacc gatccaaaat aaacaaattt
     5701 caattaaggt tggttcaacc tactggacac cccgtaacta cgaccgcagt tttagtggct
     5761 cggtgatgtt aatggacgcc ttagtccgct ctctgaatat tccaacggtg aatattggta
     5821 tgaaagttgg cttaaaaaaa gtcattgcca aacaaaaaga gatgggctgg gataatgcgg
     5881 aaattccgcc ttacccatca accttgctag gttcatattc gatctcgcct tacgatgtaa
     5941 cccgctctta tcaagtaatt gccaacgaag ggctgaaaac accattaacc accattgagg
     6001 cgattatttc gcacaatggc gaaatgttct atcagcgaga tagcggcaaa gtgagcgagc
     6061 aggtgcttcc accggaagct gcggttcaaa caatgtatgc gatgcaacag gttgtggaac
     6121 gaggtacggc tcgtagctta caaaacgatt ttgccaaact gaaattagcc ggcaaaaccg
     6181 gtacaaccaa taacgctcga gatacttggt ttgtgggaat tgatggcgaa cacgtcacaa
     6241 ccgtctggct gggtaaagat aacaatgggg ataccaacct caccggttca agtggtgcat
     6301 tgtatgtgta taagcaatat cttgagagag ccttgcctca ggcgtataga ttaccgaaac
     6361 ttaatacgat gcaatgggtg gggattaata gctatggtag ctggaattgc gatagtggca
     6421 gacaaattcc agtttggcga gataaagggc aacgttattg taccagtgca ggtaatgtag
     6481 tggctacaga aaaaccaagt gtttgggatg cactttcgct gaaaaaagaa aatatcgagg
     6541 ctaaagcaga agaagctgaa gggaagcaac caatccctac tgaagaagtt gcaccaattg
     6601 aataaaaata aatttgatag ccattatcat tcaagataca caagctgata gattctacct
     6661 ataaaaagta tatattataa ctatttgcta g
//
LOCUS       DANLXS010000134         6489 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.135, whole genome shotgun sequence.
ACCESSION   DANLXS010000134 DANLXS010000000
VERSION     DANLXS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.135"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            60..221
                     /locus_tag="PXD71_001967"
     CDS             60..221
                     /locus_tag="PXD71_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292051.1"
                     /translation="MEMSIEKIAELLSDKEKDDYPFSIEERQFAAQASADTWEAILNN
                     TIQPFDFKK"
     gene            229..591
                     /locus_tag="PXD71_001968"
     CDS             229..591
                     /locus_tag="PXD71_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292052.1"
                     /translation="MYQVFIAEPKDPNLEKGLLKHFIKSYLTQEELDFLHKILTNLPN
                     IRPFWGDTGYKYIVIDNVYFLYEVSESETKVILLGTKFNNKRKTFNFANTKTQQKSMK
                     KWINDLAIYKPHKINQSQ"
     gene            604..1521
                     /gene="nagK"
                     /locus_tag="PXD71_001969"
     CDS             604..1521
                     /gene="nagK"
                     /locus_tag="PXD71_001969"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDL6292053.1"
                     /translation="MAIYYGLDVGGTKIELAAFNEKLEKLHSERVPTPQTNYQDWLTA
                     IKTLVETADTKFDEKGTVGLGIPGFVNRETGIAEITNIRVADGKTILKDLAECLGREV
                     RAENDANCFALSEAWDESNKPYSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
                     QLNYHALKLLDWDKAPIYECGCGNQACLDTYISGRGFELLFNDLVGEKISAKAIIERF
                     YQQETKVVAFVEKYIELMAISISNLITILDPDMIVFGGGLSNFDYIYQALPKALPKHL
                     MRSAKVPVIKKAIHGDSGGTRGAAALFLK"
     gene            complement(1592..3541)
                     /locus_tag="PXD71_001970"
     CDS             complement(1592..3541)
                     /locus_tag="PXD71_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL6292054.1"
                     /translation="MYQRSQSRTVLFPILLFVGINLVILTLSRLVLGVWQSDRVSAVD
                     GWIPLILQGIRIDISSLCWLFGLPALFSVLCFHKNVLGSIWQTVLRIWLTVGSVFILF
                     MEVATPNFIETFDLRPNRLFIEYLVHPREVFTMLINGHLWAVICGIGIAMISLIIYWK
                     LSGWATKNLHFPSWKMRPLLALLVAAIVFVGGRSSFAHRGINPAMVAFSSDPLVNSLV
                     LNSGYSVLYAVQQLKDEDKSSEQYGKMSVEEMFNIVKQVRNRPASDYISDEIPTLTQN
                     KATYQGKPKNIVIILEESLGAQFVGSLGGKNLTPNLDKLSEQGWYFTNLYATGTRSVR
                     GIEAVTAGFTPTPARSVVKLTKSQTNFFSIAELLKRQGYHTSFIYGGEKHFDNMASFF
                     YGNGFQQIIDEKDYKNPKFTATWGVSDEDLFDKANETFNQLHKSGKPFFSLVFSSSNH
                     DPFEFPDGKIELYEQPKQTRHNAAKYADYAIGHFFELAKKSEYWQDTVFLVIADHDSR
                     AVGEHLVPIQHFHIPALLLGEHIQPRTDNRLVSQLDMPTTLLSVAGISGQYPMIGYDL
                     TQDIDPNRAFMQYDATQAMMRGNDVVVMRPNLPIEGFSYDKANYKLTPKEQPESFKKE
                     ALAHALLGSYLYKNQLYKLPEETKQ"
     gene            complement(3574..4362)
                     /locus_tag="PXD71_001971"
     CDS             complement(3574..4362)
                     /locus_tag="PXD71_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDL6292055.1"
                     /translation="MQVTELNIYPIKSTQAIQVSQAFVEPKGLNFDREFMLAEPNGKF
                     ITARKDPELYRLAAFPIATGVMITHTSGQKCVALYQDFVEEQSSEVWGTHFNAKVAAE
                     AVNQWLSEIFGRAVQLRWLGSQSQRIVANFADHPMSFADSNPVSLMSLKSLEQVQQWS
                     PIPLEMARFRPNIVIDGNVAFEEEQWQQVQIGEVLFTKAALCTRCILITRDLNTLELD
                     PKSEPFRTLKEKHCDEKGKPVFGIHLVPQNSGVIKVGDPVIIQL"
     gene            4487..4717
                     /gene="xseB"
                     /locus_tag="PXD71_001972"
     CDS             4487..4717
                     /gene="xseB"
                     /locus_tag="PXD71_001972"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL6292056.1"
                     /translation="MAKKPTQDFESTLKELEAIVTHLESGDLPLEEALNEFETAIKLV
                     QQGQERLQKAEQRIQILLNKNDTAPLSDYERE"
     gene            4723..5616
                     /gene="ispA"
                     /locus_tag="PXD71_001973"
     CDS             4723..5616
                     /gene="ispA"
                     /locus_tag="PXD71_001973"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDL6292057.1"
                     /translation="MSYLLATDLTHLQQRINHFLAEKLKTTDSVGSPLVEAMAYAVLL
                     GGKRVRPFLIYATGRMLGVPLEKLDHSAAAMEAIHAYSLVHDDLPAMDDDRLRRGKPT
                     CHIAFDEATAILAGDALQSFAFELLATDPHLTDREKVVQVTELATASGAKGMCLGQSL
                     DLIAENKAVSLEELELIHRNKTGALILSSVMMGLNLSEYSQNVAIKQPLVNYAKAIGL
                     AFQVQDDILDVIGETDKIGKIVGSDEHLNKSTYPKLLGLDGAKQKAEMLYQTALHSLE
                     QLPFDTTALKELANFIVKREN"
     gene            complement(5651..6451)
                     /locus_tag="PXD71_001974"
     CDS             complement(5651..6451)
                     /locus_tag="PXD71_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDL6292058.1"
                     /translation="MCQLLGMNCNTPTDITFSFDGFRRRGGLTDHHTDGFGIAFFEGK
                     GVRVFRDNRPGASSPMAEAISQYQIKSLNVIAHIRKATRGDVNLENTHPFIRELWGEN
                     WVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCRIVSELKEMYPAKPSEKEIFDAVVQ
                     ITSRIAEHGVFNFVLSNGHWMIARCTTNLHYVCRKAPFGKALRDDDVSIDFSQYTKIT
                     DKVTIIATQPLTKNENWTKMKNGGYVFFKDGDVVYEIEGTLPEAQPHV"
BASE COUNT         2028 a         1365 c         1285 g         1811 t
ORIGIN      
        1 tagccaaatc aatatacaat acaaattatt gaataagtac ctaaaataag gagcttagaa
       61 tggaaatgtc gatagaaaaa atagctgaac tattaagcga taaagaaaaa gatgactatc
      121 cgtttagtat tgaggaaaga caatttgccg cccaggctag tgctgatact tgggaggcaa
      181 tcttaaataa cactattcaa ccttttgact tcaaaaaata ggcttgctat gtatcaagtt
      241 tttattgcag aaccaaaaga cccaaactta gagaaaggct tattgaaaca cttcattaag
      301 tcttatctta ctcaagaaga actcgatttc ctgcataaaa ttctcactaa tttacccaac
      361 atcagacctt tttggggcga taccggttac aaatatatcg tcattgataa tgtttacttc
      421 ttgtatgaag tttccgaaag tgaaacgaaa gtcattctac ttggcaccaa atttaataat
      481 aaacggaaaa cttttaattt tgcgaatacg aaaacccagc aaaaatcaat gaagaaatgg
      541 attaatgatt tagcaattta taaaccgcat aaaatcaatc aatcgcaata agagagcgta
      601 attatggcaa tttattatgg cttagatgtg ggcggcacaa aaattgaatt agccgctttt
      661 aatgaaaaac tagaaaaact acacagcgaa cgtgtgccga caccgcagac aaattatcaa
      721 gattggctga cggcaattaa aacgctggtt gagactgccg atacaaaatt tgacgaaaaa
      781 ggcactgtcg ggctgggcat tccgggcttt gtaaaccgtg aaaccggcat tgcggaaatt
      841 accaatattc gagtggctga cggcaaaacg attctgaaag atttagccga atgtttaggg
      901 cgtgaagtgc gtgccgaaaa cgatgctaac tgctttgccc tgtctgaagc gtgggacgaa
      961 agtaataagc cttattccac cgtattaggg ttgattatcg gcaccggttt tggcggtggt
     1021 tttgtgtttg acggtaaaat tcattcaggc agaatcggaa tggcaggcga agttgggcat
     1081 atgcaactta actaccacgc acttaaattg ctcgactggg ataaagcccc aatttatgaa
     1141 tgtggttgtg gcaaccaagc ctgtttagac acctatattt cagggcgggg ttttgagtta
     1201 ctgtttaatg atttagtagg agaaaaaatc tcggcgaaag cgattatcga acgcttctac
     1261 caacaagaaa ccaaagtggt ggcatttgtc gaaaaatata ttgagctaat ggcaatcagt
     1321 atcagcaatc tgattacgat tttagatccg gatatgattg tattcggtgg cggtttatcc
     1381 aactttgatt atatctacca agctctacca aaagcattac caaaacactt aatgcgttca
     1441 gcgaaagtgc cggtcatcaa aaaagcaatt cacggcgact caggtggcac tcgtggtgca
     1501 gcagcattat tcttaaagta atgaacaaat ttgagagagg gcaagcggtc atttttaata
     1561 aaaatattgc agccctctct tttaatttgc cttactgctt agtttcttcc ggtaatttat
     1621 ataactgatt tttatataaa taactgccaa gcaatgcgtg tgctaacgcc tcttttttaa
     1681 acgattccgg ctgttcttta ggggtgagtt tatagttagc tttatcgtag ctaaatcctt
     1741 ctatcggtaa atttggtcgc attaccacca catcatttcc ccgcatcatc gcctgtgtcg
     1801 catcatattg cataaaggca cggtttgggt caatgtcttg cgttaaatca taccctatca
     1861 tcggatattg accacttatt cccgccacag aaagtaatgt tgtcggcata tcgagctgac
     1921 tcactaaacg gttatccgtt cttggctgaa tgtgttcgcc taataaaagt gccggaatat
     1981 ggaagtgttg aattggcact aaatgctcgc caacggctct tgaatcgtga tctgcaatca
     2041 ctaaaaacac cgtatcttgc cagtactctg attttttcgc taactcaaag aaatgcccga
     2101 tagcataatc ggcatatttt gccgcattat ggcgagtctg cttcggttgt tcatagagtt
     2161 caattttgcc gtccggaaat tcaaacggat cgtggttact ggaactgaat accaaactaa
     2221 aaaacggttt acctgatttg tgcagttgat taaaagtttc atttgcctta tcaaacagat
     2281 cttcatcact cacgccccaa gttgcggtaa atttagggtt tttataatct ttttcatcga
     2341 taatttgctg gaaaccatta ccgtagaaaa agctcgccat attatcaaaa tgcttctccc
     2401 caccataaat aaaggaagta tggtatcctt gacgttttaa taattcggca atactaaaaa
     2461 agttagtttg gcttttcgtc agttttacca cagatcttgc cggtgttggt gtaaaaccag
     2521 ccgttaccgc ttcaattcct ctcaccgaac gagtacctgt tgcataaaga ttggtaaaat
     2581 accagccttg ttctgaaagt ttatctaaat ttggagtgag atttttccct cctagcgaac
     2641 cgacaaattg agcccctaaa ctttcttcta agataatcac aatgttttta ggctttcctt
     2701 gataagtcgc tttattttgc gtgagggtgg gaatttcatc tgaaatataa tctgaagctg
     2761 gtcgattacg aacctgctta acaatgttga acatttcctc cacagacatt tttccatact
     2821 gttcggaaga tttatcttca tctttcaatt gctgaaccgc ataaagcacg gaataacctg
     2881 aatttagcac cagtgaattg acaagcggat cgcttgaaaa tgccaccatt gccggattaa
     2941 tccctcgatg ggcaaaactg gaacgcccac caacaaaaac tattgcagca accaataaag
     3001 ccagtagcgg acgcattttc cagctcggga aatgcaaatt tttggttgcc caacccgata
     3061 atttccaata aatgatgaga gaaatcatcg caatgccaat cccacaaata acagcccata
     3121 aatgtccgtt aatcagcatt gtgaacactt ctctcggatg gaccaaatac tcaatgaaaa
     3181 gacgatttgg gcgaaggtca aaggtttcaa taaaattcgg tgtggcgact tccataaaaa
     3241 gaataaatac actaccgaca gttagccaaa ttcttaaaac agtctgccaa atcgaaccca
     3301 gtacattttt atgaaaacac aacacgctaa ataatgccgg taagccaaat agccaacata
     3361 gcgaagatat atcaatgcga ataccttgta gtattaatgg tatccatcca tcaacagcgg
     3421 atactcgatc agattgccaa acgcctaaca ctaaacggga taaggtgagg ataactaaat
     3481 taatcccaac aaatagtagg attggaaata gcacagttct tgattgactg cgttggtaca
     3541 ttttgaatct ccttttaaaa aaatatcggc atattataat tgaataatga caggatcgcc
     3601 tacttttatc acaccactgt tttgtggcac aaggtgaata ccgaacaccg gcttaccttt
     3661 ttcatcgcaa tgcttttctt ttaaggttct aaacggctcc gatttggggt ctaactcaag
     3721 cgtgtttaaa tcccgtgtaa tcaaaatgca acgagtacat aatgccgctt tggtaaatag
     3781 cacttcgcca atttgaactt gttgccactg ctcttcttca aatgccacat tgccatcaat
     3841 cacaatattc gggcgaaaac gagccatttc gagtggaatc ggcgaccatt gttgcacttg
     3901 ctcaagcgat tttagagaca tcaaactcac cgggttgcta tctgcaaaac tcattgggtg
     3961 atcggcaaaa ttcgcaacaa ttcgttggct ttgcgagcca agccaacgta gttgcacagc
     4021 cctaccaaaa atttcactca gccattgatt gaccgcttct gccgccactt tggcattaaa
     4081 atgtgttccc cacacttcac tactctgctc ttccacaaaa tcttggtaaa gtgcaacgca
     4141 tttttgaccg cttgtatggg taatcattac ccctgtcgca atcgggaaag ccgctagacg
     4201 gtataattcc ggatctttac gggcagtaat aaatttgccg ttaggctcgg ctagcataaa
     4261 ttcacggtca aaatttaagc ctttcggttc aacaaatgcc tgtgaaactt gaatagcttg
     4321 agttgatttt atcgggtaaa tattgagctc ggtaacttgc atagaaaatc ctccatttgg
     4381 aaataggata acaatacctg caagcggtcg aaaagtaaaa ggaatttgca aatcccttgt
     4441 gagaacggta aacttgagca acttttacag caataggtca gcaacaatgg caaagaaacc
     4501 cactcaagat tttgaaagca cacttaaaga attagaagca atcgttaccc atttggaatc
     4561 cggtgattta ccgcttgaag aagccctcaa cgaatttgaa actgcaatca aattagtgca
     4621 acaagggcaa gaacggttac aaaaagccga gcaacgtatt caaatcttat taaataaaaa
     4681 cgatactgcc ccacttagcg attacgaaag agaataatca caatgagcta tctattagca
     4741 accgatctga ctcacctgca acaacggatt aaccattttc tcgctgaaaa attaaaaaca
     4801 acggatagcg ttggttcgcc tttagtagaa gcaatggctt atgcggtatt gctaggcggt
     4861 aaacgggtgc gaccgttttt aatctatgcc accggcagaa tgcttggtgt gccgttggaa
     4921 aaattagatc atagtgcggc ggcaatggag gctattcacg cttactcgct ggttcacgat
     4981 gatttaccgg caatggacga tgatagatta cgccgtggca agccaacctg ccatattgcc
     5041 tttgatgaag ctaccgctat tttagccggt gatgcgttac aaagttttgc gtttgaactg
     5101 ctggcaaccg atccgcattt gaccgaccga gaaaaagttg ttcaagttac agaattggca
     5161 accgcttccg gtgcaaaagg aatgtgctta gggcaaagtt tggatttgat tgcggaaaat
     5221 aaagcggtga gcttggaaga gcttgaatta attcatcgca acaaaaccgg ggcattgatt
     5281 ctatcatccg tgatgatggg attaaacctt tccgaatatt cgcaaaatgt agccataaaa
     5341 caaccgcttg taaactatgc caaagcgatt ggcttagcct ttcaggtgca agatgatatt
     5401 ttagatgtca ttggcgaaac agataaaatc ggcaaaatag tgggttcaga tgaacactta
     5461 aataaaagca catacccgaa attgttgggc ttagacgggg caaaacaaaa agccgaaatg
     5521 ctttatcaaa ccgctctcca ttcgctggaa cagttgcctt tcgataccac cgcattaaaa
     5581 gagttagcta attttattgt taagcgtgaa aactaagaaa aaacctagcc gaagctaggt
     5641 tttttagtga ttaaacgtga ggctgagctt ccggcaatgt gccttcaatt tcatatacca
     5701 catcgccgtc tttaaagaaa acatagccac cgtttttcat tttggtccag ttttcatttt
     5761 tggtaagtgg ctgagtggca ataatcgtca ctttatcggt aattttggta tattgactaa
     5821 aatcaatcga cacatcatca tcacgcaagg ctttgccaaa cggtgcttta cggcaaacgt
     5881 aatgcagatt agttgtacaa cgggcaatca tccaatgacc gttggataaa acaaaattaa
     5941 ataccccgtg ttccgcaatt cttgaggtga tttgcaccac ggcatcaaaa atctcttttt
     6001 cgcttggttt agccggatac atctctttta attccgacac aatacggcaa aatgccgctt
     6061 cggaatcggt tgtaccaatc ggctggtagt ggcactcctc gcatacccca acaccttcca
     6121 ctgtgccgtt atgggcaaac acccagttct cgccccataa ttcacgaatg aaggggtggg
     6181 tattttccaa attcacatca ccacgcgtag ctttacgaat atgtgcaata acattgagcg
     6241 atttaatttg atattggctg attgcttctg ccatcggcga tgatgctccc gggcggttat
     6301 cacgaaaaac ccgaacccct ttgccttcaa aaaaggcaat tccaaaccca tcagtatggt
     6361 gatccgtcag cccaccccga cggcgaaatc catcaaaaga aaaagtaata tcggttggcg
     6421 tattacaatt cattccaagt aattgacaca taatttgctt cttaaaacca aataatttac
     6481 aacaaaaaa
//
LOCUS       DANLXS010000135         6487 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.136, whole genome shotgun sequence.
ACCESSION   DANLXS010000135 DANLXS010000000
VERSION     DANLXS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.136"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            292..2823
                     /locus_tag="PXD71_001975"
     CDS             292..2823
                     /locus_tag="PXD71_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292059.1"
                     /translation="MQREYEKLGASISSVSRKRTEENRALKDSRHQQAKQLLYFRSLR
                     RELRASGINIKAFAQSETNLTDKIAKANQLIDQQKAKLEKLNAVKARNEKYWATVEKV
                     KNTSERLHNLGQRSMISGAAISAPVVGLGKGVVGMAQTAGKFEQFNAILEVTEGSAEK
                     AKQSFDWVKQFAVDTPSNLDEAMEAFVKLRAYGLDPTNGLLQTLGDTGAAMGKPVMQA
                     VEAIADALTGENERLKEFGIKGSVVKGTNIIEYAYTDKLGKQQVAKVNKNNRKEIEQT
                     LTRIFNEKYAGAMEKQAKTLVGIWAKIEDYWTNFQMQVMESGAFDWIKNKLQWVLDSL
                     DKMAENGELQKWAEDVGAVIQETVQGLWAFGEKIIEVVKWLASLARENKGLIATFVKW
                     SAILGSSLTVLGAFAVVASFALYPVARLALGIGKFTGASTLLNKALFDSNDKFRLFNK
                     SLYSSGTTAAFVKSKFNQLATLPNVLGSSFNKLGGGIKMLIPQLKTKVFWLNMLKGIL
                     GKLWSAIRFIFSPLKLLLGIFSPIGLAITAISVAGVALYRNWEKVRAFFGGFLQGLSK
                     GLAPVLERFKPLGKLFETISNWVKSTFKWLSDLLTPADKTSESLDKASSAGEKFGLAL
                     AGAIDLVTSPVQWLVDQFKWISENMPSWDTIGEGVKKAATAIVPTEHAKQITKTAEMS
                     NNMYDPNYTPPQLEQKWIGGLVGNGKGRGFATGGYTGNGGKYEPAGIVHKGEYVMTKE
                     ATARLGVANLNRLNYGKVAGLTALASSVAFAQPMPAVKIDSRPPLTASQPSPTVAPVS
                     QNIHITINATGGQDPQAIARLVAMELEKQQRQAQARARSSLRDRG"
     gene            2885..3064
                     /locus_tag="PXD71_001976"
     CDS             2885..3064
                     /locus_tag="PXD71_001976"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292060.1"
                     /translation="MMFKEKGYDEFVNECVRKGREEHKAGLGFTLEQSRLHIKKTIEQ
                     KSQELNEFQREVVYG"
     gene            3057..3344
                     /locus_tag="PXD71_001977"
     CDS             3057..3344
                     /locus_tag="PXD71_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6292061.1"
                     /translation="MAKITIKPQALSQFDEIVKSVIEFTGFETSGIRLQSDIFDKIEQ
                     IAFMPLSGRAREDGTRETFVRRYRIVYDYDRQADEVIILAIIHSSRLYPRP"
     gene            3473..4078
                     /locus_tag="PXD71_001978"
     CDS             3473..4078
                     /locus_tag="PXD71_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL6292062.1"
                     /translation="MSEPNHAEQIRRLDNIIRLGTIAEVDLTTATARVNSGGITTDFL
                     PWLTFRAGTSQTWSAPTVGEQCVVLSVSGEFTTGVILFGIYTQNAPSQSADEHLFRFS
                     DGAEISYNTASGQLTVKNCKVVIVQATESITLDTPTVKATKNVVIGGNLSVKGTTSSQ
                     GAISTQGAVTAKGDVSGAGISLGSHHHIEQGDGRPTSGAKP"
     gene            4078..4413
                     /locus_tag="PXD71_001979"
     CDS             4078..4413
                     /locus_tag="PXD71_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL6292063.1"
                     /translation="MNRETGEQLEDEVEHIKQSIKDILLTAKGSRVMRRTYGSNLYKL
                     TDKPIAASLIMQLSAACVMALQQWEPRIAITRFKVEFAEDNYNKLICTLDFTLKHRKI
                     SIKGMRFTL"
     gene            4410..5327
                     /locus_tag="PXD71_001980"
     CDS             4410..5327
                     /locus_tag="PXD71_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDL6292064.1"
                     /translation="MSEIVDLSKLPAPKVLEELDYETLLAERKAKFLSLYPESERAVM
                     AARLALESEPITKLLQENCYLQLLERQRINSAAQATMLAYATGTDLDVIAANFNVERL
                     VVQAEDLTASPPIVEELEPDDDFRQRVQLKIESVSVAGPRKAYTFHALSANGKIADVS
                     VISPQPAYVTVTLLSRDGNGAVASEIINAAKIALNDEEVRPIADRVTVQGASIVEYEI
                     NAVLHLYRGPEKEPILKAAKANLEAYINQTKRIGRDVTHSGIHAALHVAGVQNVEILQ
                     PRTDLVLTPYQAGYCVNYTLNVEVSDEYS"
     gene            5314..5946
                     /locus_tag="PXD71_001981"
     CDS             5314..5946
                     /locus_tag="PXD71_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDL6292065.1"
                     /translation="MNTVKALLPPGSSLLEKRAAECLQQAVQNPIKFANLINPHKCPV
                     KFLPYLAWALSVDYWEEHWSEELKRETIIRSFEEHRKKGTASAVRRIVESLGYAFEIR
                     EWFAEKRQRQPGTFRVFVELKDKGISEKIYQELERLIEDAKPVSRHITELAVSATSKG
                     KANIFSGAHSGEIITIYPPKKLPVIVIKSKMDLFSATNSGEIITIYPNKR"
     gene            5949..>6487
                     /locus_tag="PXD71_001982"
     CDS             5949..>6487
                     /locus_tag="PXD71_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292066.1"
                     /translation="MEQKYKTILTHHGERVIVEALANKIPVPLKEMAIGDGNGSSITP
                     SASQTTLVREVYRAEITDLLEDPQNRHQMIAELLIPENVGGFIVREIGLFDEQGGLVA
                     VANCPENYKPVLEQGSGKVQYYRMILQVSSSDAVTLSINNNIVYATRTEFNEFVNNLS
                     SPDGYKHIGRCKSVAELRT"
BASE COUNT         2150 a         1220 c         1475 g         1642 t
ORIGIN      
        1 ttatctcaat ctttaaaagt acagagagat gaattaaaag cattggaaaa aacacaaagt
       61 aaaatgcaat catttaaaaa aatggacgat agcattaaga aatccaatga aactatactc
      121 aaacagacaa agcatttaga taatctcaaa aataaaatca gccaaatgaa aaatgaacga
      181 attgagctga aagtgaaaat ggatgcgaaa aaaagggaat ttcaacaaat tacgaaaggt
      241 ggttcagctt ggagtgcaag agccgcattt ttagataaag attttgaaaa aatgcaaaga
      301 gaatatgaaa aactcggtgc atctatttca agtgtcagcc gaaaaagaac agaagaaaat
      361 cgggcattaa aggattctcg ccatcaacaa gctaagcaac ttttatattt tcgtagttta
      421 agacgtgaat tgagagcaag cggcattaat attaaagctt ttgctcaaag cgaaaccaac
      481 ttaacagaca agattgcaaa agcaaatcaa ttaatcgacc aacaaaaagc aaaattagaa
      541 aaactaaatg cggttaaagc tcgaaatgaa aaatattggg caactgtcga aaaggtaaaa
      601 aataccagcg aacgtttgca taatttagga caacgctcaa tgattagtgg tgcggcaatt
      661 tctgcccctg ttgttgggct agggaaaggg gttgtcggaa tggctcaaac agcgggtaaa
      721 tttgaacaat ttaatgctat tttagaggta accgagggaa gtgctgaaaa agccaagcaa
      781 agttttgatt gggttaaaca atttgcagta gataccccct caaaccttga tgaagcaatg
      841 gaagcctttg taaagttaag agcatacggt cttgatccta ccaatggctt actacaaaca
      901 ttaggtgata caggtgctgc aatgggtaag cctgtaatgc aagcagttga ggcaatagca
      961 gatgctttaa cgggtgagaa tgaacgctta aaagaatttg gtattaaagg aagtgtagtt
     1021 aaaggtacaa atattattga gtatgcctac actgataaat taggcaaaca acaagtcgca
     1081 aaagtaaaca aaaataaccg aaaagaaatt gagcaaaccc taacccgtat ttttaacgaa
     1141 aaatatgctg gagcaatgga aaaacaagct aaaacgctag ttggtatttg ggcaaaaatt
     1201 gaagattatt ggacaaattt ccaaatgcaa gtgatggaat cgggagcatt tgactggatt
     1261 aaaaataaat tgcaatgggt gttagacagt ttagataaaa tggctgaaaa tggcgagttg
     1321 caaaaatggg ctgaagatgt cggagccgtt attcaagaaa cagtacaagg cttatgggct
     1381 tttggtgaaa aaattattga agtggttaaa tggttagcga gcttggcacg ggagaataaa
     1441 gggctaattg caacattcgt taaatggtct gctattcttg gctctagttt aacggtgcta
     1501 ggagcttttg cagtggtggc aagttttgca ttatatcctg tagcaagatt ggctcttggt
     1561 atcggcaaat ttacaggtgc aagcacctta ttaaataagg cattatttga tagtaatgat
     1621 aaattccgat tattcaataa atcactttat tcatcaggca ctacggctgc ttttgttaaa
     1681 tcaaaattta atcagttagc aactttacca aatgtgctgg gaagtagttt taataaatta
     1741 ggcggtggca tcaaaatgct aattccacag cttaaaacaa aagtattttg gctcaatatg
     1801 ctaaaaggta ttttaggcaa gttgtggagt gcaatccgtt ttattttctc accgttgaaa
     1861 ttattacttg gtattttctc gccgattggc ttagcgatta cagctatttc ggtagcaggg
     1921 gtagcgttat atagaaattg ggagaaggtc agagcctttt ttggaggctt tttgcaagga
     1981 ttatctaaag gtcttgcacc agtattagag agatttaaac ctttaggaaa actatttgaa
     2041 actatatcaa actgggttaa atctacgttt aagtggttat ccgatttatt aaccccagcg
     2101 gataaaacat cggaaagttt agataaagcg agttctgcag gagaaaaatt cgggcttgca
     2161 ttggctggtg caatagactt ggtaacatca cctgttcagt ggctagtcga tcagtttaag
     2221 tggatttctg aaaatatgcc gagttgggac actattggtg agggtgtaaa aaaggcagct
     2281 actgcaattg ttcccaccga gcacgccaag cagattacta aaacggcaga aatgtcaaac
     2341 aatatgtatg accccaatta tactccacca caacttgagc aaaaatggat tggtggcttg
     2401 gttggtaacg gtaaaggaag aggttttgcc acaggcggct acaccggcaa tggtggcaaa
     2461 tatgagcctg ccggtattgt gcataagggc gagtatgtaa tgaccaaaga ggcaactgca
     2521 cgcttgggtg tggcaaatct taaccggtta aattatggca aagttgctgg gctaactgca
     2581 cttgcgtcaa gtgttgcgtt tgctcaacca atgcctgcgg tcaaaatcga cagcagacca
     2641 ccgctgacgg caagccaacc aagcccaaca gttgcaccgg tgtcgcaaaa tatccatatc
     2701 accatcaacg ccaccggtgg gcaagaccca caagcaatcg cccgactggt tgcaatggag
     2761 ttggaaaaac aacaacgcca agcacaagca agagcaagat cgtctttgag agacaggggc
     2821 taatttgaca aagtagaatg aaaagcggac aatgctctaa attgtccgct ttttatagga
     2881 gcaaatgatg tttaaagaaa aaggatatga tgaatttgtg aatgaatgtg tgcgaaaagg
     2941 tagagaagaa cacaaagctg gtttaggatt tacgctagag caatctcgct tacatatcaa
     3001 aaaaaccatt gagcaaaaat cacaagagct aaacgaattt cagcgtgaag tggtttatgg
     3061 ctaaaattac cattaaacca caagcattga gtcaatttga cgagattgta aaaagtgtta
     3121 tcgaatttac cggttttgaa acgagtggta ttcgattaca aagtgatatt tttgacaaaa
     3181 tagagcaaat tgcatttatg ccactctctg gaagagcaag agaagatggg acaagagaaa
     3241 ctttcgtcag acgttatcgt attgtgtacg attacgatag gcaggcagat gaagtaatta
     3301 tcttagctat cattcacagt agccgacttt acccacgccc ataatcagta agcgacctaa
     3361 tcggtcgctt tttctttgcc tgttaaaacc catttcacac ctcgcattta tacccaaagc
     3421 ctttcgcctt ttgcaaaata tcctcatttt ttaaccgctt gtaattgtaa aaatgagtga
     3481 gccaaaccac gccgaacaaa tccgccgttt agacaatatt atccgacttg gcactattgc
     3541 cgaggtggat ttaaccacag cgactgcacg ggtcaattcg ggcggtatta cgaccgattt
     3601 cttaccgtgg ctgacttttc gtgccggtac atcgcaaacg tggtcagctc caacagttgg
     3661 cgagcaatgc gtggtgttaa gcgtgagtgg tgaatttacc acaggcgtga tcttatttgg
     3721 gatttacacc caaaacgccc cgagccaatc cgccgatgag catctattcc gcttttcgga
     3781 cggtgcagaa atcagctaca acaccgcaag cggtcaatta accgtaaaaa attgcaaagt
     3841 ggtgattgtg caggcgacag aatctatcac gctcgacacg cccacggtta aggccacaaa
     3901 aaatgtcgtg attgggggca acctatccgt gaaaggcaca acctcatcgc aaggtgcaat
     3961 cagcacacaa ggggcggtaa cagcaaaagg cgatgtatca ggggcgggaa tcagtttagg
     4021 cagccaccat cacattgagc aaggtgacgg cagaccaact agcggagcaa aaccataatg
     4081 aaccgagaaa caggcgaaca gcttgaagac gaagtagagc atatcaagca gtctatcaaa
     4141 gatattttgc tgacggcaaa aggcagtcgg gtaatgcggc gaacctatgg cagtaatctc
     4201 tacaagctaa ctgataaacc gattgccgca agtctgatta tgcaactctc cgctgcgtgc
     4261 gtgatggcgt tgcagcaatg ggagccacgt atagcgatca cccgatttaa agtggaattt
     4321 gccgaagata actacaataa gctgatttgc accttagatt ttacccttaa gcaccgaaaa
     4381 atcagcatca aaggaatgcg ttttacacta tgagcgaaat tgtcgattta tccaaactcc
     4441 ccgccccgaa agtactggaa gaattggact atgaaacctt actggcagag cggaaagcca
     4501 agtttttatc cctctaccca gaaagcgaac gagcggtaat ggctgcacgg ttagcgttgg
     4561 agtctgagcc tatcaccaag ttactacaag aaaattgcta tttgcagcta ctcgaacgcc
     4621 aacgcattaa tagtgcggct caagccacaa tgcttgctta tgctaccggt acggatttag
     4681 atgtcatcgc agccaacttt aatgttgagc ggcttgtagt gcaagctgaa gacttaactg
     4741 caagcccgcc tatcgtcgaa gagttagagc ctgatgatga tttccgtcag cgtgtgcagt
     4801 taaaaattga aagtgtttcg gttgccggtc cacggaaggc ttacaccttt cacgccctgt
     4861 ctgctaacgg aaaaattgcc gatgtgtccg tcatatcacc acagccagct tatgtaacgg
     4921 taacgctgct ttctcgtgat ggcaacggag ccgtagcctc agaaatcatc aatgcggcca
     4981 aaattgctct caatgatgaa gaggttagac cgattgccga tagagtaacc gtgcaagggg
     5041 caagcattgt ggaatatgaa attaatgcgg tgttgcatct ctaccgtgga cctgagaaag
     5101 agcctatttt aaaagcggca aaagctaact tagaggctta catcaaccaa acaaaacgca
     5161 ttggacgaga tgtgactcac tcaggtatcc acgccgcctt gcacgttgcc ggtgtgcaaa
     5221 atgtggaaat tttgcagccg agaactgatt tggtgctgac accttaccaa gccggttatt
     5281 gtgtcaatta cacgctaaat gttgaggtga gcgatgaata cagttaaagc attattgcca
     5341 cccggttcat cacttttgga aaaacgagct gccgaatgtt tgcaacaagc ggtacaaaat
     5401 ccgattaaat ttgcaaattt gattaatcca cacaaatgcc ccgtgaaatt tttgccctat
     5461 cttgcgtggg ccttatcggt ggattattgg gaagagcatt ggagtgaaga attaaaaaga
     5521 gaaaccatta ttcgatcttt tgaagaacac cgaaagaaag gcacagcaag tgcagtgcga
     5581 agaattgtgg aatctctcgg ctatgctttt gaaatccgtg agtggtttgc ggaaaaacgg
     5641 caacggcagc ccgggacgtt tagggttttt gttgagttaa aagataaagg gattagcgaa
     5701 aaaatctacc aagaattaga aaggttgatt gaagatgcaa aaccggtatc aaggcacatc
     5761 acagaattag cagtatcagc cacctcaaaa ggcaaagcaa atatttttag tggagcacac
     5821 agtggcgaaa ttattacgat ttatccacct aaaaagctcc ctgttattgt cattaaaagc
     5881 aaaatggatt tatttagtgc aacaaacagt ggcgaaatta ttacgattta tcctaataag
     5941 aggtaaaaat ggaacaaaaa tataaaacaa tattaaccca tcacggtgag cgagtgatcg
     6001 ttgaggctct tgcaaataag ataccagtgc cacttaagga aatggctata ggagatggta
     6061 acgggtcatc aataactcca agtgcatctc aaaccacact tgtaagagaa gtttatcgtg
     6121 cggaaattac agatttgcta gaagacccgc aaaatcgcca tcagatgatt gctgaattac
     6181 taatcccaga aaatgtaggt ggatttattg tccgagaaat agggttattt gatgaacaag
     6241 gtggtttggt tgctgtagca aattgccctg aaaattataa gcctgtatta gaacaaggca
     6301 gtggtaaagt ccaatattac cgaatgattt tacaagttag tagtagtgat gcggtcacct
     6361 tatcgattaa caataatatt gtttacgcca caagaactga gtttaacgaa tttgtaaata
     6421 acttgagtag tcctgatggc tataaacaca tcggacgctg caaatctgta gcagaactcc
     6481 gcactat
//
LOCUS       DANLXS010000136         6457 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.137, whole genome shotgun sequence.
ACCESSION   DANLXS010000136 DANLXS010000000
VERSION     DANLXS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.137"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..707)
                     /locus_tag="PXD71_001983"
     CDS             complement(<1..707)
                     /locus_tag="PXD71_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6292067.1"
                     /translation="MSIYRRKANGPWWVDITTPNGKRIKRSTRTLVKREAQQYHDKLK
                     QEMWAEDKLEEKRKYIFEDALLHYVRAAEELKDKATKKRHAEYWLSKFAGRELSSLTA
                     QEIILNIPKKNANTKKPLSHSTQNKYVKSLSRVLNIAHKLGMLDAVPHIPKKKEPPIR
                     VRWITKEQAKQLIDKLSSDWMKSICKFALMTGARRTEILTMTWDKIDLVRKVAIVTND
                     VAKSGKARSLLLNQEAI"
     gene            complement(611..934)
                     /locus_tag="PXD71_001984"
     CDS             complement(611..934)
                     /locus_tag="PXD71_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6292068.1"
                     /translation="MEHLTINQVAKELNCHPNTVRQHLADWGFFKMVGSEVWRINRED
                     LERAKKRAGIDPIRKRSDNDRGLSLSVEENEKCRSTDEKQTVRGGLISQPQTARELRE
                     ALARL"
     gene            complement(1015..1182)
                     /locus_tag="PXD71_001985"
     CDS             complement(1015..1182)
                     /locus_tag="PXD71_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL6292069.1"
                     /translation="MQFTYGSICSGIEATSIAWHGLGKPLWFSEIEPFPCAVLAHRFP
                     NVPNGNGMAVP"
     gene            complement(1190..1564)
                     /locus_tag="PXD71_001986"
     CDS             complement(1190..1564)
                     /locus_tag="PXD71_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292070.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEECQIRCPKTYAKL
                     REILEANEENVVRIGNLSISIG"
     gene            1714..1941
                     /locus_tag="PXD71_001987"
                     /pseudo
     CDS             1714..1941
                     /locus_tag="PXD71_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1966..2361
                     /locus_tag="PXD71_001988"
     CDS             1966..2361
                     /locus_tag="PXD71_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292071.1"
                     /translation="MKIHTQIRKEILSLLEAKLTDIEHFYNGQPNFIDIDEQQLAISV
                     YLDEINRQELTLCDEQWTAQLNITIYLKSVDEAEDELDEWAEKIREVVETYSAFEHLE
                     GISLSQYQYEQDQNQRTWHSATLIFDVEY"
     gene            2393..3034
                     /locus_tag="PXD71_001989"
     CDS             2393..3034
                     /locus_tag="PXD71_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292072.1"
                     /translation="MAKTTKVQGTKFRIGIGREAQKAITAITLATATLTIATSGYKKG
                     DAIEITGCGQLDGIYPVLSVTGDQVKLCEEVNWTGKDLPANYTKAKAALVQYSDQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPSTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            3117..3518
                     /locus_tag="PXD71_001990"
     CDS             3117..3518
                     /locus_tag="PXD71_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292073.1"
                     /translation="MTLREKLLANKPKLQSIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGALLFNPLNIDDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            3563..3793
                     /locus_tag="PXD71_001991"
     CDS             3563..3793
                     /locus_tag="PXD71_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL6292074.1"
                     /translation="MPERHFAEYQLFYQEQPFGLWREDYRAAQLAHLTAMINRDPKGK
                     APELSEFMPFFNRADEVEANEDDGVADYLAKR"
     gene            3861..4127
                     /locus_tag="PXD71_001992"
     CDS             3861..4127
                     /locus_tag="PXD71_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL6292075.1"
                     /translation="MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAK
                     TKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEAFHKAMLAE"
     gene            4131..4400
                     /locus_tag="PXD71_001993"
     CDS             4131..4400
                     /locus_tag="PXD71_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL6292076.1"
                     /translation="MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKY
                     RDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVRLGSHAELFG"
     gene            4475..4738
                     /locus_tag="PXD71_001994"
     CDS             4475..4738
                     /locus_tag="PXD71_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292077.1"
                     /translation="MKNVLLFIVHFFLIAMLSIIPILIILGAIERDPYYTAWIFLSVF
                     LGIGALLYFATIVSKLINKGKTQTLEEKRYAEYWNNVKVNIPK"
     gene            4798..5508
                     /locus_tag="PXD71_001995"
     CDS             4798..5508
                     /locus_tag="PXD71_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL6292078.1"
                     /translation="MSGLGKLTVTLELENAKFQSAMTKSDYEAQKFAKNFIQNMDRAR
                     NHAKEFADRSTQYLKNIEQAAKNLNKNSEFSFLSTIGGHYQSMSSGILSAAKAYTDIQ
                     NKMKLVSGSSAEAAKRLTDVFDIATKTSQSTEAVSGVYQTFAQNAQSLGLAQKDVAEL
                     TKTVSQAVAASGASSSAASNALTQFGQSLLMGKMKAQEFNSLITQTPTIIQAMAKGLG
                     MTMAEFKAAVYRAKSLLG"
     gene            5486..5776
                     /locus_tag="PXD71_001996"
                     /pseudo
     CDS             5486..5776
                     /locus_tag="PXD71_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            5766..>6457
                     /locus_tag="PXD71_001997"
     CDS             5766..>6457
                     /locus_tag="PXD71_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL6292079.1"
                     /translation="MKFSKYQLLVNKICFICGKKDCPQMKKSKDYKDFLDALEKGDVD
                     KADKIYNTKFSQFSKSFGHEMEKNLEKNRIPPIYQGVPFDSFDLYQHYSYGNGEALNL
                     SSVGLSNKIRPLVNKDKAFGKSNGSIESRFISQYKNEGRTSFSNAYDFTKEASGIADP
                     LWALGTATISGELKDIQIFPGGIARGNIHYSIRDRFTDPFDTFNFTKGEWNPNGTPYI
                     IQDSWIKSVRFS"
BASE COUNT         2049 a         1275 c         1247 g         1886 t
ORIGIN      
        1 ttaattgcct cttgattcag taatagcgat cgtgccttac ctgatttagc gacatcatta
       61 gtcacgattg ctacctttct tacaagatct atcttatccc aagtcattgt caaaatttct
      121 gtccgtctgg ctccagtcat caaagcaaac ttacagatag atttcatcca gtctgaactt
      181 aatttatcta ttagctgttt tgcttgttct ttggtgatcc agcgtacacg aattggcggc
      241 tctttctttt tcggtatgtg tgggactgca tccaacatac caagtttatg agcgatattt
      301 aatactctcg ataaagattt cacatactta ttttgcgtac tatgagatag aggctttttc
      361 gtatttgcat tctttttcgg gatatttagg ataatctcct gagctgttaa agagctgagt
      421 tcacgaccgg caaatttgga taaccagtat tcagcatgtc gcttttttgt ggctttatct
      481 tttaattctt cggccgcacg aacgtaatgg agcaaagcat cttcaaagat atattttctc
      541 ttttcctcga gtttatcctc tgcccacatt tcttgcttga gtttatcgtg atactgctga
      601 gcttcacgtt ttacaagcgt gcgagtgctt ctcttaattc tcttgccgtt tggggttgtg
      661 atatcaaccc accacggacc gtttgctttt cgtctgtaga tcgacatttt tcattctcct
      721 cgaccgacag agataaccct cggtcattat cagatcgttt tcttattggg tcaataccgg
      781 ctctcttttt tgctctttcc aaatcttctc ggttgatccg ccaaacttcc gagcctacca
      841 ttttaaaaaa tccccaatcc gccaaatgtt ggcggactgt atttggatgg caatttaatt
      901 cttttgccac ttgattaata gtaagatgtt ccattcatta ctttctccaa aataaatccc
      961 tctaaacgag ggattgtact aaataatcgt taagtctttg ccttatccac tgcatcaagg
     1021 cacagccata ccgtttccat ttggcacatt ggggaaacgg tgagctaata ccgcacaggg
     1081 gaatggctca atttcagaga accacaaagg cttacctaaa ccgtgccacg ctatacttgt
     1141 agcttcgata ccgctacaaa ttgagccgta ggtgaattgc attttttacc tatcctatcg
     1201 aaatactcaa attcccaata cggacaacat tttcttcgtt ggcttctagg atttctctca
     1261 acttagcata ggttttgggg catcttattt gacattcctc ttcgttgtag agagcctcta
     1321 atgtgtgcca ttcttttgca agtgcagccc aaactttccc aagttcagcc attttttcaa
     1381 tcctatttcg caaaaaaggg actgtctcca acaacagaaa gcaacgcctg aaatccgata
     1441 catcgtgtgg gtagccttct atttttggtc ttattccgta accaaggtaa aaagccattg
     1501 ttttactgct tacccctgtc tctccattgg caagccacca agcgattttg tcttctatgt
     1561 gcatttttcc tccaaaaatt aaccgcttgc ggaacaagtt ctacaagcgg ttgtcaagtg
     1621 ttcatttgtt caatgaacag ttcattgtga ataacaaaac cgcttgaatt acaagcggtc
     1681 tattttgttg cgtaaattta ctgttagcgt tgagccataa ccgactacga ccgctggccc
     1741 gaagatattg acggcgaggg ggacggcttc tcgcttgcct ccaaacggac gaccactttt
     1801 atgtcagccg gaatgacttt agtggagagt tcgcctggta aagatattgt cgatctcaaa
     1861 catatcccga aaagtagcca cgaagcccca ccgactaccg gtattttaag cctctacaat
     1921 cgaggcgacc gcagacgctt tagcgaaaca gttgataggt aagctatgaa aatccatacc
     1981 caaatccgaa aagaaatcct ttcattatta gaggcaaaat taacggacat cgagcatttt
     2041 tataacgggc aaccgaattt cattgatatt gacgaacagc aacttgccat ctctgtttat
     2101 cttgatgaga ttaaccgtca ggagcttacc ttatgcgatg aacagtggac agctcaactt
     2161 aacatcacga tttatctaaa atcagttgat gaggcagaag atgaattaga tgagtgggcc
     2221 gaaaaaatcc gtgaagtggt tgaaacatat tctgcgtttg aacatttaga gggcatcagc
     2281 ctttctcaat atcaatacga acaagatcaa aatcaaagaa cttggcactc agccacattg
     2341 atttttgatg ttgaatacta acaagcaaat aatccttata ggagaatcta caatggcaaa
     2401 aacaacaaaa gtccaaggca caaaatttag aattggcatt ggacgtgaag cccaaaaggc
     2461 aattacggct atcactttag ctacagcaac gcttactatt gccacatcag gctataaaaa
     2521 aggcgatgca atcgaaatta ccggttgtgg tcagttagac ggcatttatc ctgtattatc
     2581 tgtaacaggc gatcaagtca aattgtgcga agaagtaaac tggacgggta aagatttacc
     2641 ggctaattac accaaagcca aagcagcttt agtacaatac tcagaccaat tctgtgcggt
     2701 aaaaaacatt gaaaaatctg atgatacgtt aagtaccgaa gatgttacaa cggtctgctc
     2761 ggaaggtaca gaaaccgaac ccggagaaat tgaatttggc tcaattaaac tgagcttttt
     2821 tcacaagcca agtacggaaa tgcaaactcg cttacgcaaa ttgttttatg acaaatccac
     2881 ctttgcgtac aaattagaat tacctgataa ccacggcaca acctatggcg aaggtttcat
     2941 tgaggcgggt aacggcttta gtggcgaagt taaaggcaaa tatgaaggct cggtatcaat
     3001 taaaccaagc aaacgtgatt atttgctagt gtaattacaa aatattgctc caaatcgacc
     3061 gcttgtaata ttgcaagcgg tcttttttat cctaaatttt acaaaaggaa tcgacaatga
     3121 cactccgtga aaaactcctc gccaacaaac caaaattaca atctatcgaa atcaacggtg
     3181 agacctacta cctgcgtgaa gccactgttg gcgatatgaa taagcagatt tttgaaaccc
     3241 gaagctggct cattcaacaa gctgaacaag aaaatgttga attaccggca gaagatgatg
     3301 aaacctttga cgaagctctc aaccgttttg gcgaaaaata ccgccttgct caatcggttg
     3361 cctaccgttt atgtgacgaa aacggtgcat tactgtttaa cccacttaac attgacgatc
     3421 tcaatgcgat tgccgaatta gacagtaaag tgattatcga ctttaaccaa gccgtgtccg
     3481 ccccaaaaga ctcagcgagc gaagaaagtt ccaaataacc ctttcgctcg cactgggtaa
     3541 aacccttgaa gaaatcgaac aaatgcctga acgccacttt gccgaatatc agctttttta
     3601 tcaagagcaa ccttttgggc tttggcgtga ggactatcgt gctgctcaac ttgcccactt
     3661 aaccgcaatg atcaaccgtg acccaaaagg caaagcccct gaactctcgg aatttatgcc
     3721 gttttttaat cgggctgatg aagtagaagc aaatgaagat gatggcgttg cggattattt
     3781 ggcgaaaagg taaatctctt gtatttcatt gtaattctgt ctataattgg tgtaatacat
     3841 agaaatacag gagagataaa atggcgacta tcaatgatgc tttcagtttt agaacaaata
     3901 ccgaaataaa aaataccgca tttgatgtaa ttaaaaacta tggaatgaca ccctctcagg
     3961 tgtttaatat gtttttaacc gagattgcaa aaacgaaaac tattccgtta agtttgaatt
     4021 atcaacccaa tcttgaaaca aaattggcaa tgcaagaagc aaaatcaggt aaaaatgaag
     4081 tttatgcctc acttgaagca tttcataaag caatgttagc ggagtaaata atgctacaaa
     4141 tttcgccgac aaacgcatat aaaagagact ttaaaaagat tgcagccgaa ttagtcggta
     4201 gttcggaata tgtggaagta atgtattgcc taataaacca attaccattg gcggaaaaat
     4261 atagagatca cccactacaa ggtgaatggc aaggctttag agattgccat attaagcctg
     4321 atttagtgtt gatttacgct gttgaagata atctgctccg ccttgttcgt ttaggatcgc
     4381 acgctgaatt atttggataa tttctgtaaa aactaaccgc ttgcgatttt gtgctgtggg
     4441 cggtatagtt ggaatattaa caaaggaggg aaaaatgaaa aatgtacttc tatttatcgt
     4501 acactttttc ttaatcgcaa tgctctcaat tatcccaata ttaattattc ttggagcaat
     4561 cgaaagagat ccttactata ctgcgtggat tttccttagc gtatttttag gcattggagc
     4621 attgttatat tttgcaacca ttgtttctaa gttaattaac aagggaaaaa cacaaacatt
     4681 agaagaaaaa cgatatgcag aatattggaa taatgtaaaa gtcaatattc ccaaatagtc
     4741 taactgataa atgaccaaaa gctcgcttta tgcgggcttt tttattggag aaaaacaatg
     4801 agtggcttag gaaaattaac cgttacctta gagcttgaaa atgcaaaatt tcagtccgca
     4861 atgacgaaat ctgattatga ggcacaaaaa tttgccaaga actttatcca aaatatggat
     4921 agagctagaa atcacgccaa agaatttgcc gatcgctcaa cccaatattt aaaaaacatt
     4981 gagcaagcgg caaagaacct caataaaaat tctgaatttt catttttatc gactattggt
     5041 ggacattatc aatcaatgtc tagtggcata ttgagtgcgg caaaagccta tactgatatt
     5101 caaaataaaa tgaaattagt aagcggtagt tcagcagagg cagctaaacg attaactgat
     5161 gtgtttgata ttgcgactaa aacaagccaa agtacagagg ctgtttcagg tgtctatcaa
     5221 acatttgctc aaaatgccca atctctcgga ctggctcaaa aagatgtagc agaattaact
     5281 aaaacggtct ctcaagccgt tgctgcaagc ggcgcaagca gttctgcagc aagtaatgcc
     5341 ctaacccaat ttggtcaatc acttttaatg ggcaaaatga aagcccaaga atttaactcg
     5401 ctcattactc aaaccccaac cattattcaa gcaatggcaa aaggtttggg aatgacaatg
     5461 gcagaattta aagcagcggt ataccgggca aaatctttac ttggataaca ttcaagggca
     5521 aggttttgag agagtggata caccccaaat cggcgatgtg attttaatac aggtcggttc
     5581 tgatgtgccg aatcacgcag cgatttatat tggtgaacaa atggtaatcc accatagccc
     5641 gaaccgatta tctaagcgtg atttatatga cggttattgg ttacgccata cccacagtat
     5701 ttggcggcat aaactggcgg atagattgga ttttgacggc atatttaatg atataggaga
     5761 acaatatgaa atttagtaaa tatcaattat tagttaataa gatctgcttt atttgtggta
     5821 aaaaagattg tccacaaatg aaaaagagta aagactacaa agactttttg gatgctttag
     5881 aaaaagggga tgttgataaa gcagataaaa tatacaatac gaagttttca cagttttcta
     5941 aatcttttgg tcatgaaatg gaaaagaatt tagagaaaaa tcgtatacct cctatttatc
     6001 agggagttcc atttgattct tttgacttat atcagcatta ttcttatggt aatggagagg
     6061 cattaaatct ttcatccgtt ggattaagta ataaaattcg tcctctagtg aataaagata
     6121 aagcatttgg taaatccaat ggaagtatag aaagccgatt tatttcgcaa tataaaaatg
     6181 aaggacgcac atctttctcc aatgcttatg attttacaaa agaagcatcg ggtattgcag
     6241 atccgttatg ggcgttagga acagctacca ttagtggaga attgaaagat atacaaattt
     6301 ttcccggtgg tattgctagg ggaaatatcc attatagtat cagggacaga ttcactgacc
     6361 catttgatac attcaatttt actaaaggag aatggaaccc aaatggaaca ccttatatta
     6421 ttcaagatag ctggataaaa tcggtacgat tttcgat
//
LOCUS       DANLXS010000137         6448 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.138, whole genome shotgun sequence.
ACCESSION   DANLXS010000137 DANLXS010000000
VERSION     DANLXS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.138"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..911)
                     /locus_tag="PXD71_001998"
     CDS             complement(<1..911)
                     /locus_tag="PXD71_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HDL6292080.1"
                     /translation="MKWLMLCREPRLYSCCRIKAACEAKGIELDILDQNRMLLGLENG
                     EFKIYYQAGESYDNNRPEPVLLPNYDGILPRFGTGSTEMGCSVLRHFEAKGIPVLNHS
                     AAFALARDKWRTLQKLAKHNLPVPNTNFAGHLVSVKSQLNQFAFPLISKVLNGSQGNG
                     VMLFEGKNNAEAVLATFRQVNEPYLCQQFVGDAKGQDIRAFVIGNEVVAAMSRTSVTG
                     DFRANIHQGGTAQPIELTQMERALAIKATKTIGLDIAGVDFLRTEKGVVILEVNASPG
                     FEGIERVNDVDIASEMVSYFINKMKNA"
     gene            complement(908..1627)
                     /gene="nfsA"
                     /locus_tag="PXD71_001999"
     CDS             complement(908..1627)
                     /gene="nfsA"
                     /locus_tag="PXD71_001999"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HDL6292081.1"
                     /translation="MQSKPTLETILSHRSIRKFTEQPISEEIFQILIEAGKAASSSNH
                     LQCVTVIRVTDRQIRETLKAVSGMQYVVDCAEFLVFCIDFNKHKQLVPESQLEWAEVS
                     LIGAVDTGILAQNVFLAAESLGLGAVYIGALRNDIERVSKALNLPEYCVPLVGMCLGY
                     PAQEPAIKPRLPNSLVCYNNQYQDFDWTEFAKYNENLTAYYQARIGEPQQWQAAIHKT
                     LDKPVRPHILPFFNQKGLLKK"
     gene            complement(1639..2424)
                     /gene="mtgA"
                     /locus_tag="PXD71_002000"
     CDS             complement(1639..2424)
                     /gene="mtgA"
                     /locus_tag="PXD71_002000"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDL6292082.1"
                     /translation="MEQSMAKRNKRFLSSLLKKIGLFFIPRKIKSKLGWLWFGSSRLL
                     TFGGTFFLIPILIFSVLPVPYSAYMVQKKVENLVQGKSYPIKKEWVSLNEISWQMQMA
                     VIASEDQKFESHFGIDLQAIETALKLNAKSKKVRGGSTISQQTVKNMFLWHGQSWLRK
                     GIEVPLTLLVENVWSKDRILEVYLNIAEFGNGIFGVEAAAKHFFKKSAKQLTLQESAL
                     LAASLPNPLVFRVDKPGPSMRKRQAWIMRQVSLLDGKNYLDKL"
     gene            complement(2399..2719)
                     /gene="trpR"
                     /locus_tag="PXD71_002001"
     CDS             complement(2399..2719)
                     /gene="trpR"
                     /locus_tag="PXD71_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDL6292083.1"
                     /translation="MKTLYNQRDPNEWTQFLALLEQAVAEDKLEPFFSMFLTADERGS
                     LGLRLQIVQALLKGEDSQREIQQNLNTSAATITRGSNMLKTLDQDFLQWLNGKVNGTV
                     NGKA"
     gene            complement(2799..3440)
                     /gene="lolA"
                     /locus_tag="PXD71_002002"
     CDS             complement(2799..3440)
                     /gene="lolA"
                     /locus_tag="PXD71_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDL6292084.1"
                     /translation="MKTLFKKSVFGLALFGLANVALADAQSVAELQRRLEQVSQYSAD
                     FDQTVRSSKGKQIQQGKGKFQVKRPNLFRMDTKSPQENLIVSDGKDLWFYDPFVSQVT
                     VNTVQDAVNNTPFVLLTSGDKSHWDQYDVSQSADTFVLKPKSKKSNLKQFDVRIDQSG
                     LLKGFSTIERDGQSNLYVLRNITTGGVSSDLFKFSVPKGAELDDQRGGKKSKK"
     gene            complement(3551..6409)
                     /gene="polA"
                     /locus_tag="PXD71_002003"
     CDS             complement(3551..6409)
                     /gene="polA"
                     /locus_tag="PXD71_002003"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL6292085.1"
                     /translation="MATIAKNPLVLVDGSSYLYRAFHAFPPLTNRLGEPTGAMYGVLN
                     MLKSLISQVNPSHIAVVFDAKGKTFRDELFKQYKSHRPPMPDDLRPQIEPLHRIIKAL
                     GIPLISIEGVEADDVIGTLAVQAAKDGKDVLISTGDKDMAQLVNDHIMLINTMNNTLL
                     DREGVIEKYGIPPELIIDFLALMGDSADNIPGVKGVGEKTALALLQGIGSLEQIYANL
                     DQIATLSFRGAKNFAPKLEAEKANADLSYLLATIKTDVELDVTHDQLLTQPQDRAELA
                     QLFEHYEFKRWLNEVNQNANPVTQTSAEKVPNNYQVTQAVRNEENVANVVEIDRSKYE
                     TVDTEQKLSAWIEKLTAARLIAVDTETDSLDAMSANLVGISFALENGEACYIPLGHKQ
                     KAQPQQADMFGDEERSESPSSDELVKNQLNLTACLSKLKPILLNPEIKKIGQNIKYDL
                     TIFANYGIELQGVAFDTMIESYTQNSTGRHNMDDLAARYLGHKTIPFEELAGKGKNQL
                     TFDKIEIAKAAEYAAEDADVTMKLHQVLWEKLQQEPELVKLFDEIEMPLVGVLSRIER
                     NGVLIDPKKLLTQSAEIEKRLEEVETLVHAEAGQTFNLASTKQLQEILFEKLGLPVLK
                     KTPKGAPSTNEEVLDELAQQGHLVPKLLMEHRGLSKLKSTYTDKLPQLINKKTGRVHT
                     SYNQAVTATGRLSSSDPNLQNIPIRNEEGRRIRQAFVAREGYKIVAADYSQIELRIMA
                     HLSNDEGMINAFAEGKDIHRATAAEIFGVELGEVTSEQRRSAKAINFGLIYGMSSFGL
                     SNQLGIGRAEAQKYMDLYFQRYPAVQQFMTDIREVAVEKGYVETLFGRRLYLPDIKSG
                     NAILRKAAERVAINAPMQGTAADIIKVAMIGIDNAIRDNDEIKMIMQVHDELVFEVKA
                     DKIDHYSQLIKTEMEKAISLKVPLIAEVGVGENWDEAH"
BASE COUNT         1754 a         1502 c         1219 g         1973 t
ORIGIN      
        1 caagcattct tcatcttatt gataaaataa gaaaccattt cagaagctat atcaacatca
       61 tttactctct ctatgccttc aaatcctggg ctggcattga cttctaaaat aactacgcct
      121 ttctctgtcc gcagaaaatc aacgcctgca atatcaagcc ctatcgtttt agttgcttta
      181 attgctaacg cacgttccat ctgagttaat tcaatcggtt gagccgttcc accttgatga
      241 atattggctc taaaatcgcc tgttacgctg gttcggctca tggcagcaac cacttcattg
      301 ccaatcacaa acgcccgaat atcttgccct ttagcgtcgc ccacaaattg ttggcataga
      361 taaggctcat ttacttgcct aaaggtcgct aacacggcct cggcattatt tttgccttca
      421 aatagcatta cgccattccc ctgtgaacca tttagcactt ttgaaataag tggaaaagca
      481 aattgattga gttgtgattt cacagaaacc aaatgccctg caaaattcgt attaggaacc
      541 ggcaaattgt gtttggctaa tttttgtaat gttcgccatt tgtctcgggc taacgcaaat
      601 gccgctgaat gatttagaac aggaatacct ttcgcctcaa aatggcgtaa caccgaacag
      661 cccatttcgg tactgcctgt gccaaaacgg ggtaaaatgc cgtcataatt cggcaacaat
      721 accggctccg gacggttgtt atcatagctc tcgccagctt gatagtagat cttaaattcg
      781 ccattttcta acccgagtag cattctattc tggtctaaaa tatccagctc aatccccttt
      841 gcctcacaag cggcttttat tcgacagcag ctatataaac gaggctctcg acatagcatt
      901 agccacttca ttttttcagc aatcctttct gattgaaaaa cggcaaaata tgcggacgaa
      961 caggcttatc caatgtttta tgaatagctg cttgccattg ttgtggctcg ccgattctgg
     1021 cttggtagta agcggtcaaa ttttcattat attttgcaaa ttcagtccaa tcaaaatcct
     1081 gatattgatt gttataacaa accaaactat tcggcaaacg aggttttatt gccggctctt
     1141 gtgccggata ccccaaacac atacccacca gtggaacaca atattccggc aagttaaggg
     1201 ctttagaaac ccgctcaata tcattacgca atgctccaat atacaccgct cccagcccta
     1261 gtgattcggc ggctaaaaat acattctggg ccaaaatacc ggtatctacc gccccaatca
     1321 acgacacctc cgcccattcc aactgcgatt cagggacaag ttgtttgtgt ttattaaaat
     1381 caatgcagaa caccaaaaat tcagcacaat ctactacata ttgcatacct gaaacggctt
     1441 ttaatgtttc tcgaatctgc cgatcggtta cacgaatcac cgtcacacat tgcagatgat
     1501 ttgaacttga agccgcttta cctgcctcaa ttaagatttg gaaaatctct tcagaaatag
     1561 gctgttcggt aaatttacga atagaacggt gagataaaat cgtctcaaga gtcggtttac
     1621 tttgcatatt tttctcgtct aaagtttatc taaataattt ttgccatcaa gcaaacttac
     1681 ttgtcgcata atccacgctt ggcgttttcg catactcggg ccgggtttat ctaccctaaa
     1741 caccaacggg ttaggcaaag aggctgccaa tagtgcggat tcttgcaagg ttagctgttt
     1801 ggctgatttt ttgaaaaaat gctttgcggc tgcttccacc ccgaaaatgc cgttaccaaa
     1861 ttccgcaata ttgaggtaca cctctaaaat ccgatcctta ctccacacat tttcgactaa
     1921 cagggttaaa ggcacttcaa tccctttgcg taaccagctt tgcccgtgcc acaaaaacat
     1981 attttttacc gtttgttgcg aaatggttga acctccacgc acttttttgg atttagcatt
     2041 cagtttcaag gcagtttcta tcgcttgtaa gtcaatgcca aaatggcttt caaacttttg
     2101 atcttctgag gcaatcacag ccatttgcat ttgccacgaa atctcattca ggctcaccca
     2161 ttcttttttg atcgggtaag atttcccctg aaccagattc tccacctttt tttgcaccat
     2221 atacgcagaa taaggcaccg gtaacaccga aaaaatcagt atcggaatca ggaaaaatgt
     2281 gccaccaaag gttaataagc gactagaacc aaaccaaagc cagcccagtt tactttttat
     2341 tttgcgagga ataaaaaaaa gccctatttt cttcaaaaga gagcttaaaa atcgcttgtt
     2401 acgctttgcc attgactgtt ccattaacct tgccgttcag ccattgcaga aaatcttggt
     2461 cgagtgtttt aagcatattt gaaccacgag tgatggttgc cgcactggta tttaaatttt
     2521 gttggatttc ccgttgcgaa tcttcacctt ttagcaaagc ttgcacaatt tgtaaacgca
     2581 agcctaatga acctcgctca tcggcggtga gaaacattga gaaaaacggc tcaagtttat
     2641 cttccgcaac cgcttgttca agcagagcca aaaactgcgt ccactcgttt ggatctcgct
     2701 ggttatagag tgttttcata ctgtcccttg aagtaaaaac ggtgacaagc ggtcagattt
     2761 ttacaaaatt ttgcagaaaa acgaccgctt gctctccatt attttttcga ttttttaccg
     2821 ccacgttggt cgtctaactc tgcccctttc ggtacgctga atttaaacaa atcgcttgag
     2881 acaccgccgg tagtaatatt gcgtaacaca tataagttac tttgcccgtc acgctcaatg
     2941 gtactgaaac cttttaataa accgctttgg tcaatccgca catcaaactg ttttaaattg
     3001 ctttttttcg attttggctt aagcacgaaa gtatcagcac tctggctgac atcatattga
     3061 tcccagtggc ttttatcacc gctggttaat aagacgaatg gcgtattatt aactgcatct
     3121 tgcacggtat ttaccgtaac ttgtgaaacg aaaggatcgt aaaaccataa atctttgccg
     3181 tcagatacaa tcaaattttc ttgtggtgat ttggtatcca ttctaaataa gttcgggcgt
     3241 tttacttgga atttgccttt gccttgctga atttgtttac ctttgctaga acgcacggtt
     3301 tggtcgaaat ccgcactata ttggcttact tgttccaaac ggcgttgtaa ttctgcaaca
     3361 gattgagcat cagccaatgc cacatttgcc aagccaaata acgctaaacc gaataccgat
     3421 tttttaaata atgttttcat ttgttttcct ttttaccaac aagaattttc ttgtttgaca
     3481 ataaaatcat caaaagtttc atattaatga actgttacaa caatacaata aaagtcgtga
     3541 attgtatagt ttaatgagct tcgtcccagt tttcgcctac gccaacttcg gcaatcagtg
     3601 gcactttgag gctaatcgct ttttccattt cggttttgat taattggctg taatggtcga
     3661 ttttatcggc tttgacttca aacaccaatt catcgtgaac ctgcataatc attttgattt
     3721 catcattatc acgaatcgca ttatcaatac caatcatcgc caccttaata atatcggcag
     3781 ccgtgccttg cattggggcg ttgattgcca cccgttctgc tgctttacgc aaaatagcat
     3841 tgccggattt aatatcaggc aagtacaaac ggcgaccaaa tagggtttca acatagcctt
     3901 tttcaactgc aacttcacga atatcggtca taaattgttg aaccgccggg taacgttgga
     3961 agtagagatc catatatttt tgagcctctg cacgcccaat ccctaattgg tttgaaagcc
     4021 caaatgacga cattccgtaa atcagcccga agttaattgc tttcgcactg cggcgttgtt
     4081 cggaggtgac ttcacctaac tccaccccga aaatttcggc agcggtagca cggtgaatat
     4141 ctttgccctc cgcaaaggcg ttaatcattc cctcatcatt cgataaatga gccataatcc
     4201 gtagctcaat ttgtgaatag tcggcagcga caattttata accttctctc gcgacaaaag
     4261 cttggcgaat ccgtctgccc tcttcattgc ggatcggaat gttctgcaaa ttcggatcgc
     4321 tggaagaaag gcgtccggtt gcggtgactg cttgattgta agaggtatgc acccgccctg
     4381 tttttttgtt aatcagttgt ggtaatttat cggtgtaggt agatttcaac ttacttaacc
     4441 cacgatgctc cattaataac ttcggcacta aatgcccttg ttgagccaac tcgtctaaca
     4501 cttcttcgtt ggttgagggt gcaccttttg gagttttctt caacaccggc aagcctaatt
     4561 tttcaaacaa aatctcttgt aattgtttag ttgaggcaag attgaaggtt tgccctgctt
     4621 ctgcgtgtac taatgtttca acttcctcca aacgtttttc aatttcagcc gattgggtca
     4681 ataatttttt cgggtcaatc aacacgccat tgcgttcaat tcgagaaagt acgcccacaa
     4741 gtggcatttc aatttcatcg aataacttca ccaattcagg ctcttgctgt agtttttccc
     4801 acaatacttg gtggagtttc atcgtcacat cggcatcttc tgccgcatat tcagccgctt
     4861 ttgctatctc gattttatcg aaggtcagtt gatttttccc cttaccagca agctcttcaa
     4921 acggaatcgt tttatgcccc aaataacggg cggctaaatc gtccatatta tgacgaccgg
     4981 tgctgttttg ggtgtaagat tcaatcatcg tatcaaaagc aacaccttgt agctcaatgc
     5041 cgtagttcgc aaaaatggtt aaatcatatt taatattctg cccgatcttt ttaatctccg
     5101 ggttaagcaa aatcggcttg agtttactta aacaagcggt caaatttagc tgatttttta
     5161 ccaattcatc ggaagacggg ctttctgaac gctcctcatc accgaacata tcggcttgtt
     5221 gcggttgggc tttttgctta tgcccaagcg gaatatagca cgcctcgcca ttttccagtg
     5281 caaaggaaat accgaccaaa ttagccgaca ttgcgtcaag gctgtcggtt tcggtatcca
     5341 ccgcaatcaa acgagctgcg gtgagttttt cgatccaggc tgataatttc tgttcagtat
     5401 caacggtctc atatttgcta cgatcgatct cgaccacatt tgcaacattt tcctcatttc
     5461 tgaccgcttg tgttacttgg taattattcg gtactttttc agccgaagtt tgagtgaccg
     5521 gattggcatt ttgattcact tcgttcagcc agcgtttaaa ttcataatgc tcaaataatt
     5581 gagcaagctc agcacggtct tgcggctgtg ttaaaagttg atcgtgagta acatcaagtt
     5641 ccacatcggt tttgatggta gcaagcaaat aggataaatc ggcattcgct ttttccgctt
     5701 ccaattttgg ggcgaaattt tttgccccac ggaaagaaag ggtggcaatt tgatccaaat
     5761 tagcataaat ctgttcaagt gaaccaatgc cttgcaataa agctaatgcg gtcttttccc
     5821 ccacaccttt tacgcccgga atgttatcgg cggaatcgcc cattaatgcc agaaagtcga
     5881 taatcaactc aggcggaatg ccatattttt caatcacgcc ttcacgatct aacaaagtat
     5941 tgttcatcgt gttaatcaac ataatgtgat cgttcaccaa ttgtgccata tctttatcgc
     6001 cggtgctgat caacacatct ttgccgtctt tagcggcttg cacagcaagc gtgccgatca
     6061 catcatcagc ttccacacct tcaatcgaaa tcagtggaat gcctaaggct ttgataatgc
     6121 gatgcaaagg ctcaatttga gggcgtaaat catcgggcat tggtgggcgg tgcgatttat
     6181 attgcttaaa caactcatca cggaaggttt tccctttggc atcaaacacc acagcaatat
     6241 ggcttggatt cacttgtgaa atcaggcttt ttaacatatt cagcacgcca tacattgccc
     6301 ctgtcggttc gcctaagcga ttagtgagcg gagggaaagc gtgaaatgct cgatataaat
     6361 aggaagaacc atccaccaat accagtggat tttttgcgat agttgccata aaaatctctt
     6421 tataatcaaa taaatattgg cgttatta
//
LOCUS       DANLXS010000138         6413 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.139, whole genome shotgun sequence.
ACCESSION   DANLXS010000138 DANLXS010000000
VERSION     DANLXS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.139"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..270)
                     /locus_tag="PXD71_002004"
     CDS             complement(<1..270)
                     /locus_tag="PXD71_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6292086.1"
                     /translation="MSINISPLASKNLTEWKLLWQQYLDFYQVNLADDIIQSTWQKVS
                     ESENVKGFGAFVDNRMVGFVHIVIHPNTWNLTECCYLEDLFVLAEY"
     gene            complement(337..714)
                     /locus_tag="PXD71_002005"
     CDS             complement(337..714)
                     /locus_tag="PXD71_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HDL6292087.1"
                     /translation="MKNPIEIYQSQDGKTQVSVQFDKETVWLSLQQMADIFGRDKSVI
                     SRHLRNIYQEGELEREATVAKNATVQIEGAREVVRLKDYLVKGYALNERRLQERGIEF
                     EQVIGLLSQYTGCLSLVGGRKFA"
     misc_feature    complement(736..858)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(988..1977)
                     /gene="rsmC"
                     /locus_tag="PXD71_002006"
     CDS             complement(988..1977)
                     /gene="rsmC"
                     /locus_tag="PXD71_002006"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDL6292088.1"
                     /translation="MLSLESEVLERHLALFENKSVLLFGDVRDDFARQLQSRAEKVAV
                     FSSYFDYARHHSEVEFGLECGLSAELAVFYWTKNKQECQFQLLQWLSQCQAGQEMLII
                     GENRAGVRSVEKLLEPFGNIAKIDSARRCGLYHFELQTVPTFDCKKFWKSYRLQDLDV
                     FDLPAVFSSAELDNGTKLLLSTFDKADRLKGKVLDLGCGAGVIGATLKQQFPKIKLTM
                     SDIHAMALESGRRTLAENQLEGEVVASDVFSHINERFDLIVSNPPFHDGVDTAYRAVE
                     ELIFQAKNHLTKGGELRIVANAFLPYPDLLDKAFGKHDVLAKSTKFKVYSART"
     gene            2107..2529
                     /locus_tag="PXD71_002007"
     CDS             2107..2529
                     /locus_tag="PXD71_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDL6292089.1"
                     /translation="MNRRDLLLQEMGISQWVLTNPQVLKGDAQIRLNNAVKLVVVCEE
                     DHQHSRLFADILRALKLKNSEYQWLTGEQSQRLVFEHSPIFWLIQAEEQAVKMAKSFT
                     NQTAWKNESWQDLAKSTTKRQLWQQIDTFSAQFGEQRD"
     gene            2522..2959
                     /gene="rimI"
                     /locus_tag="PXD71_002008"
     CDS             2522..2959
                     /gene="rimI"
                     /locus_tag="PXD71_002008"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL6292090.1"
                     /translation="MIEQIQAEDFEQLFEIEQQAHLVPWSKGTLLNNQGEKYLNLKVV
                     EQNKIVAFAISHIVLDEATLFNIAVAPDFQGKGIGKRLLSELILQLQQRKLTTLWLEV
                     RESNHSARKLYDSLGFNEVTTRKNYYPTPNGSKENAVVMALYL"
     gene            complement(3008..4855)
                     /gene="typA"
                     /locus_tag="PXD71_002009"
     CDS             complement(3008..4855)
                     /gene="typA"
                     /locus_tag="PXD71_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL6292091.1"
                     /translation="MQNVDISKLRNIAIIAHVDHGKTTLVDKLLQQSGTFGETRGDVD
                     ERVMDSNDLEKERGITILAKNTAINWNGYHINIVDTPGHADFGGEVERVLSMVDSVLL
                     VVDAFDGPMPQTRFVTQKAFAHGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLGASD
                     EQLDFPIIYASALNGVAGLEHEDLAADMTPLYEAIVKHVQPPQVELDAPFQMQISQLD
                     YNNYVGVIGIGRIKRGSVKPNQAVTIVDSEGKTRQGRIGQVLGHLGLQRFEATEAFAG
                     DIVAITGLGELNISDTICDINAVEALPALSVDEPTVTMFFCVNTSPFCGQEGKFVTSR
                     QILERLNKELVHNVALRVEETSNPDEFRVSGRGELHLSVLIENMRREGYELAVSRPKV
                     IYREEDGKKQEPFEQVTIDIEEQHQGSVMEALGIRKGEVRDMIPDGKGRTRLEYVIPS
                     RGLIGFRNEFMTMTSGTGLLYSTFSHYDDVKPGEIGQRKNGVLISNATGKALAYALFG
                     LQERGKLMIDHGVDVYEGQIIGIHSRSNDLTVNCLQGKKLTNMRASGKDDAIVLTTPV
                     RFTLEQALEFIDDDELVEVTPQSVRIRKRLLTENDRKRASRTTTSTSTH"
     gene            complement(5040..5717)
                     /locus_tag="PXD71_002010"
     CDS             complement(5040..5717)
                     /locus_tag="PXD71_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL6292092.1"
                     /translation="MSIAENLSQIHQQIQQISEQYQRDNVRLLAVSKTKPVEAIQAAI
                     EAGQRAFGENYVQEAVEKIEFFANRPELEWHFIGPLQSNKTKLVAAYFDWIQTVDRLK
                     IAERLNEQRPADKAPLNVLIQINISDEASKSGIEPDEMLPLAQAISQLPNLKLRGLMA
                     IPKPESEPEQQKVAFRKMQLLFNRLQTEFDDIDTLSMGMSDDMQAAIECGSTMVRIGT
                     AIFGARR"
     gene            5788..6327
                     /locus_tag="PXD71_002011"
     CDS             5788..6327
                     /locus_tag="PXD71_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDL6292093.1"
                     /translation="MLDLTKIKLVITDVDGVLTDGGMYYTDQGEVMKRFHVHDGLGVK
                     MLQSCGIKVAVLSGGDTALLRKRLEVLKIDLALLGKMEKRSACFELMQQAGVTPEQTA
                     YIGDDTLDLPAFEVCGIAIATRNALDYIKEKADWVLQKAGGEGAFREVSDQILEAQGF
                     GEIFKTADGFLTVAGKMAQ"
BASE COUNT         1802 a         1413 c         1311 g         1887 t
ORIGIN      
        1 atattcagct aaaacaaata aatcttccaa ataacagcat tcagttaaat tccaagtatt
       61 tgggtgaatc acaatatgaa caaaaccaac cattctgtta tcaacaaaag ccccaaatcc
      121 ctttacattt tcactctctg aaactttctg ccaagttgat tgaataatat catctgctaa
      181 attcacttga taaaaatcta aatattgttg ccacaatagt ttccattcag tgagattttt
      241 ggaggctaac ggcgaaatat taatactcat attttttcct gttatgcttt taataaaata
      301 aagtgttcaa tcagtttaat catcagttct ttttgttcag gcaaactttc tgccaccaac
      361 aaggctaaga caaccagtgt attgactgag caaaccaatc acctgctcaa actcgatacc
      421 tcgttcctgc aaacggcgtt cgttaagggc atagcctttc accagatagt cttttaggcg
      481 aacgacttct ctagcacctt caatttgaac tgttgcattt tttgcaacag ttgcctcacg
      541 ctccagttca ccttcttggt agatatttcg taagtgccga gaaataacag acttatctcg
      601 tccaaaaata tctgccattt gctgtaaact tagccacacc gtttccttat caaactgcac
      661 ggaaacttgg gttttgccgt cttggctttg gtaaatttca atcgggtttt tcatattatt
      721 cccaaatttt atctataaaa aaatctctcg aaagttgcct tccgagagat ctttattgat
      781 ttgctacact tgctcggaag agttatcttc cgcacacacc aaatgcccga aagatatcag
      841 gttaaagtgt ggtggtgatg taaagtgtag tttaaattca ttgcattact ccaaaacagt
      901 atgaacagaa tttacgtcaa gccggcagaa atatcaagca cttttttaag caaataccgg
      961 cttttttata accgtttgtt ctgcctatta tgttcttgcg gaatacacct taaacttggt
     1021 ggattttgcc agtacgtcgt gtttgccgaa ggctttatcg agcaaatctg ggtaaggcaa
     1081 gaatgcgttt gcgacaattc gcagctcacc gcctttggtt aggtgatttt tagcttggaa
     1141 aatcaactcc tccaccgctc ggtaggcggt atcgaccccg tcgtggaatg gtgggttgga
     1201 gacaattaaa tcaaaccgtt cattaatgtg agagaacaca tcacttgcta ccacttcccc
     1261 ctctaactga ttttccgcca aagtacgtct gccggattcc aacgccattg cgtgaatatc
     1321 gctcatcgtc agtttgattt tcggaaattg ttgtttgagc gttgccccaa tcacgccagc
     1381 cccacagcct aaatctaaca ctttgccttt tagcctgtct gctttgtcga aggttgaaag
     1441 caataatttg gtgccgttat ccagctccgc cgagctaaaa accgctggta gatcgaacac
     1501 atctaagtct tgcaagcggt aggatttcca gaattttttg caatcgaatg tcggcacggt
     1561 ctgtagctca aaatggtata aaccacaacg gcgtgcagag tcgattttgg caatattgcc
     1621 gaacggttcg agcagttttt ctaccgaacg aacaccagct cggttttcgc cgataatcaa
     1681 catttcttgc cctgcttggc attgcgacag ccattgtagc agttggaatt ggcattcctg
     1741 cttgttcttc gtccaataaa acaccgctaa ctcggcactc aaaccacatt ctaagccaaa
     1801 ttcaacctca ctatggtggc gtgcgtaatc aaaatagcta ctgaataccg caaccttttc
     1861 cgcacgagat tgcagctggc gggcaaaatc atctcgcaca tcgccaaata ataacaccga
     1921 cttgttttca aataacgcca aatggcgttc aagtacttcg ctttctaaag agagcatttt
     1981 cttaccttat ctaaaaaatt tagcgtgaat ataccgaatt taagtacaga aatcttgaat
     2041 tttttgaaaa aagtttctgt tcaagcggag ttttgatact atttgcaaaa tttgatgagg
     2101 ttttaaatga atcgtcgtga tttactgtta caggaaatgg gtatttcgca atgggtgcta
     2161 accaatcccc aagtgctaaa aggcgatgct caaatccgcc taaacaatgc ggtaaaattg
     2221 gtggtagtgt gtgaggaaga tcaccaacac agccggctct ttgccgatat tctgcgtgct
     2281 ttaaagttaa aaaacagcga atatcaatgg ctgactggcg aacaatcaca acgtttggtg
     2341 tttgagcatt cgccgatctt ttggctgatt caagcggaag aacaagcggt caaaatggca
     2401 aaaagtttta ctaatcagac cgcttggaaa aacgagtctt ggcaagattt agccaaatct
     2461 acgaccaaac gccaactttg gcaacaaatt gatacttttt cggcacaatt tggagaacag
     2521 cgtgattgag caaattcaag cggaagattt cgagcagtta tttgaaattg agcaacaagc
     2581 ccatttagtg ccgtggtcaa aggggacgtt attaaataat cagggtgaaa aatacctgaa
     2641 tttaaaagtg gtagagcaaa acaaaattgt ggcttttgcc attagtcata tcgtgctaga
     2701 tgaagcaacc ttatttaaca ttgcagttgc tcctgatttt caaggcaaag gcattggcaa
     2761 gcggttactt tccgaattaa ttttgcaact gcaacaacgg aaactcacca ccctgtggtt
     2821 ggaagtgcga gaatccaacc attctgccag aaaactctac gattcactcg gttttaatga
     2881 agttaccacc cgcaaaaact attaccccac gccaaacggt agcaaagaaa atgcggtggt
     2941 aatggcgtta tatttgtagg gagaatttaa aaccaaaaga gcgacctcag tcgctctttt
     3001 ttatcagtta gtgcgtgctg gtactggttg tagtacggct tgcacgtttc cggtcgtttt
     3061 cagttaataa acgtttacga atacgcaccg attgtggtgt tacttccact aattcgtcat
     3121 cgtcaataaa ctctaacgct tgttctagag tgaaacgcac cggtgtggtt aaaacgatgg
     3181 catcgtcttt acctgatgca cgcatattgg ttaatttctt accctgtaag cagtttacgg
     3241 ttaaatcgtt tgaacggctg tgaatcccga taatttgccc ttcatataca tcaacaccgt
     3301 ggtcgatcat taattttcca cgctcttgta agccaaataa tgcgtatgct aatgccttac
     3361 ccgttgcgtt agaaattaac acaccgtttt tacgctgacc gatttcgccc ggttttacgt
     3421 catcatagtg gctgaatgta gagtaaagta aaccagtacc tgaggtcatc gtcataaatt
     3481 cgttacggaa accgattaag ccacggcttg ggatcacata ctctaaacgg gtacgacctt
     3541 tgccgtccgg aatcatatca cgcacttcac ctttacggat accaagtgct tccatcactg
     3601 aaccttggtg ttgctcttcg atatcaatcg tcacttgctc aaacggctct tgttttttgc
     3661 cgtcttcttc gcggtagatt actttcgggc gagacactgc taactcgtaa ccttcacgac
     3721 gcatattttc gattaatacc gataagtgta attcgccacg acctgacaca cggaattcgt
     3781 ctgggttcga agtttcttcc acacgcaatg ccacgttgtg gactaattct ttgtttaaac
     3841 gctcaagaat ttggcgtgaa gtcacaaatt taccttcttg accgcagaat ggcgaggtgt
     3901 ttacgcagaa gaacatcgtt accgttggtt catcaacgct taatgctggt aaagcctcta
     3961 ctgcgttaat atcacaaata gtatctgaaa tatttaattc gcctaaacca gtaatcgcaa
     4021 caatatcgcc agcaaaggct tctgttgctt caaaacgttg taaacctaaa tgccccaaca
     4081 cctgaccgat acgaccttgg cgagttttgc cttcgctatc tacaatcgtt accgcttggt
     4141 taggtttcac cgaaccacgt ttgatacgtc caataccgat aacgcccaca tagttgttgt
     4201 agtctaattg cgaaatctgc atttggaatg gtgcatctaa ttccacttgt ggtggttgaa
     4261 cgtgtttgac aatcgcttca taaagtgggg tcatatctgc cgctaggtct tcgtgttcta
     4321 agcctgctac accatttaag gctgaagcgt agataattgg gaaatctaac tgctcatcac
     4381 ttgcacctaa atttacaaac aagtcgaaca cttgatccac cacccaatca gggcgagcac
     4441 ctgggcggtc aactttattg atcactacga ttggttttaa accgtgagcg aacgcttttt
     4501 gcgtgacgaa acgggtttgc ggcatcgggc catcaaaggc atcaaccact aacagaacag
     4561 aatctaccat tgaaagtaca cgctcaacct caccaccgaa gtcagcgtgc cctggggtat
     4621 ctacgatatt gatgtgataa ccattccagt taattgccgt attttttgca agaatggtaa
     4681 tgccacgctc tttttcaaga tcgttggaat ccattacacg ctcatcaacg tcaccacgag
     4741 tttcaccaaa ggtgcctgat tgttgtaaaa gtttatcaac caaggtggtt ttaccgtggt
     4801 caacgtgggc aataattgca atgttacgca attttgaaat atctacattt tgcattggaa
     4861 tatctttaag ttaagtcaaa ataaaaactc tcgcccatca aaactggcga gagtcaagaa
     4921 attaggaggc gtaattatac agactttttc cgctgttggc tacaaaacaa gcggtggaat
     4981 ttgcaaagtt tttggcgaat ttgaccgctt gtatcagctt tatgtaaaaa ctcagctggc
     5041 taacgtctcg ccccaaaaat cgcagtccca atccgcacca ttgtcgagcc acattcaatc
     5101 gccgcttgca tatcgtccga catccccatt gaaagggtat caatgtcgtc aaattctgtt
     5161 tgcaagcggt taaaaagtag ctgcattttg cgaaaggcaa ctttttgttg ctctggctcg
     5221 ctttctggtt tgggaatggc cattaagccc cgcagtttca aattcggtaa ttgagaaatc
     5281 gcttgggcaa gtggtagcat ttcatctggt tcaatgcccg atttcgaggc ttcatcgctg
     5341 atattaattt gaatcagcac gttgagcggg gctttatccg ctgggcgttg ttcgtttaaa
     5401 cgctcggcaa tttttaatct atccaccgtt tgaatccaat caaaataggc agcgaccaat
     5461 ttggttttat ttgattgaag tggcccaata aaatgccatt ctaattcggg gcgatttgca
     5521 aaaaattcga ttttttcgac cgcttcctgc acataatttt cgccaaatgc acgctgccct
     5581 gcctcaatcg ccgcttgaat cgcctcaact ggtttagttt tggagacggc taacaaacgc
     5641 acgttatctc gttgatattg ttcggaaatt tgctgaattt gttgatggat ttgcgataaa
     5701 ttctcggcaa tcgacatttc ggttcctttg gtaaacggag gtaaaaaagc tataatggta
     5761 aaatcatacc atatttgagg caaaaaaatg ttagatctca ccaagattaa attagtgatt
     5821 accgatgtgg acggcgtgct aaccgatggc ggaatgtact acaccgacca aggcgaagta
     5881 atgaaacgtt tccacgttca cgatggttta ggcgttaaga tgttacaaag ttgtggcatt
     5941 aaagtggcag tactctcggg cggcgatacg gctttgctcc gcaaacggtt agaggtgctg
     6001 aaaattgatc tcgctttgtt aggcaaaatg gaaaaacgct ccgcctgctt tgagctaatg
     6061 cagcaagccg gtgttacccc tgagcaaacg gcttacattg gtgatgacac cttagattta
     6121 ccggcttttg aagtgtgtgg cattgcgatt gcaacccgta acgccctcga ttacatcaaa
     6181 gaaaaagcag attgggtatt gcaaaaagcc ggtggtgaag gggcgtttcg tgaagtctcc
     6241 gaccaaattt tggaggctca aggattcggt gaaattttta aaaccgcaga tggtttttta
     6301 acggtagcgg ggaaaatggc tcagtagact cttcaagcag ttaaatttca ttttattttt
     6361 gcgatctgca tcgaaaaaat gtatttctaa tctgattttc aaattactgt ttg
//
LOCUS       DANLXS010000139         6390 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.140, whole genome shotgun sequence.
ACCESSION   DANLXS010000139 DANLXS010000000
VERSION     DANLXS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.140"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            33..587
                     /gene="srlA"
                     /locus_tag="PXD71_002012"
     CDS             33..587
                     /gene="srlA"
                     /locus_tag="PXD71_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725059.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL6292094.1"
                     /translation="MIEAITHGAEWFIGLFQKGGETLVGMINGILPLLISLLVVMNAL
                     IKFVGQDRIERLAQRSAGNPISRYLILPVIGTFVFCNPMTLSLGRFLPERYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAISYLLVGMFTNFFRGWI
                     TDFTTAFFEKRMGITLDREVHLSH"
     gene            618..1619
                     /locus_tag="PXD71_002013"
     CDS             618..1619
                     /locus_tag="PXD71_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL6292095.1"
                     /translation="MSNKAIYIEKGNGGWGGPLTLPVVEGKKVLYMTGGTRPAIVNKL
                     VELTGWEAVDGFKEGEPPAEEIGIAIIDCGGVLRCGLYPKRRIPTINIHSTGKSGPLA
                     EFIVEDIYVSAVKENNIRLIDADGSVPVATEQAKPTSSTTATAKEYDSSKKITEQSDG
                     LLAKIGMGMGSVAALFFQAGRDTIDTVLKTILPFMAFVSALIGIIIASGLGDVIAHGL
                     TPLANSPIGLVTLALICSFPLLSPFLGPGAVIAQVIGVLVGTQIGLGNIPPHLALPAL
                     FAINAQAACDFIPVGLSMAEAKQDTVRVGVPSVLVSRFLTGAPTVLIAWAVSAFIYQ"
     gene            1664..2062
                     /gene="srlB"
                     /locus_tag="PXD71_002014"
                     /gene_synonym="gutB"
     CDS             1664..2062
                     /gene="srlB"
                     /locus_tag="PXD71_002014"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL6292096.1"
                     /translation="MHRLSKRENIYMTIIYQTTFTKIGNFAQESLTDDMLITFKQGAP
                     ADLQDYCFIHNPSELSSPLEVGDIAEFDGVAYPITAVGSVASENLSALGHITFRFDGA
                     NDAEFPGSVHVIGTPPQGLTENSTLIIKRD"
     gene            2110..2898
                     /gene="srlD"
                     /locus_tag="PXD71_002015"
     CDS             2110..2898
                     /gene="srlD"
                     /locus_tag="PXD71_002015"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDL6292097.1"
                     /translation="MNNEKKVAIVVGGGQTLGAFLSEGLADAGYRVVVADLNGENAQK
                     VAADINAKQGEGNAIGVQVDAADEPSVEALAKATDTVFGRADVLVYSAGTAKASPIWD
                     FNLNDFDLSVKVNLTGYFLTAKHFSRLMIRDGIKGRIIQINSKSGKVGSKYNSGYSAA
                     KFGGVGLTQSLALDLADKGITVHSLMLGNLLKSPMFQSLIPQYAAKLGIPESEVEQVY
                     IDKVPLKRGCDYQDVLNVLKFYAGEEAAYCTGQSINITGGQVMF"
     gene            3067..3447
                     /gene="gutM"
                     /locus_tag="PXD71_002016"
     CDS             3067..3447
                     /gene="gutM"
                     /locus_tag="PXD71_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDL6292098.1"
                     /translation="MDSITLLIILAMIMWLLQAVLGWLQIKAFNQAFMEISKKGKVTV
                     GRNSGRFNPKSVIVLAFDDEKKVVDSLCMKGFSVFARPQKLASVIGLSLDQIVPEKIF
                     PSDKRSQFALKVAISSGYSCSDQK"
     gene            3510..4157
                     /gene="hxpB"
                     /locus_tag="PXD71_002017"
     CDS             3510..4157
                     /gene="hxpB"
                     /locus_tag="PXD71_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDL6292099.1"
                     /translation="MQINSVIFDMDGVMIDSEPQWAEAQIAVLADLGINITEQDCEQL
                     TRGKRIDELSDTWIKKFQLSVSNQALSEAILAHGCAAIEQHGKALTGLYELLDFLKSN
                     QIKLAVATSSPPVVIKAVFDRLKLWDYFPIRCSAMDEEYGKPHPAVYLSAVKKLGIEK
                     QECIVIEDSVTGLIAAKAANLTTFLVNTDYQNEKFSIADKRMASLLDVIEKLKQH"
     gene            4200..4997
                     /locus_tag="PXD71_002018"
     CDS             4200..4997
                     /locus_tag="PXD71_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor"
                     /protein_id="HDL6292100.1"
                     /translation="MKPFERRQQILAYLAQHGRTEVDVLADYFKLTGATIRKDLTSLE
                     EDKKVLRTYGSVVLIDEEILEREEKINKEDKDDKPLAQKTGINLQQKQAIGQKAAELI
                     NEGNSIIFDAGSTVLQMIPHLKAFDNLSLMTNSLTIFNEILKLNKSYNLLISGGTFRE
                     KSASFHGYFAESAFIGSNFDILFIGTDGLDLDVGLTTFNEVYKVSSLMCDAANKIVLL
                     ADSSKFGRKSPNIVCGLEKIHTIITDEGLSQEMREQIKAKGIELLVV"
     gene            5008..5943
                     /locus_tag="PXD71_002019"
     CDS             5008..5943
                     /locus_tag="PXD71_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDL6292101.1"
                     /translation="MNHLDSAKETLNLYAQQIITLNHRLSEEFNEVVNMILACQGRVV
                     VGGIGKSGLVGKKMVATFASTGTPSFFLHPTEAFHGDLGMLKPIDMVILISNSGETDD
                     VNKLIPSLKNFGNKIIAMTGNPYSTLGRNADVILNIGVEREACLNNLAPTSSTLVTMA
                     LGDALAIALMKARDFRPEDFARYHPGGSLGRKLLNRVRDVMVRKVPTASLDTTFTECL
                     SVMNEGRMGVAVIMQDDKLEGIITDGDIRRTLAKFGAESLNKTADEIMTRNPKTINDT
                     EFLAKAEDLMKELKIHSLIAVNDEGKVTGLMEFSS"
     gene            complement(5987..6369)
                     /locus_tag="PXD71_002020"
                     /pseudo
     CDS             complement(5987..6369)
                     /locus_tag="PXD71_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1922 a         1169 c         1426 g         1873 t
ORIGIN      
        1 aaaatatttt attttgaaaa acggagatcg gtatgattga agcaattaca cacggagcag
       61 aatggtttat cggcctgttc caaaaaggcg gtgaaacctt agtcggtatg ataaacggta
      121 ttttgccact cttaatttca ttattggttg taatgaatgc cctgattaaa tttgtagggc
      181 aagatcggat tgaacgactt gctcaacgta gtgccggaaa ccctatttca cgttatttga
      241 tcttaccggt gatcggcact ttcgttttct gtaacccgat gacattaagc ttagggcgtt
      301 tcttaccgga gcgttacaaa ccaagctact acgccgcagc ctcttatagt tgccactcta
      361 tgaacggctt attccctcac atcaacccgg gtgaattatt cgtttattta ggtattgcca
      421 gcggtttaac caccttaggc ttaccacttg ggcctttagc catcagttat ttattagtcg
      481 gtatgtttac caatttcttc cgtggctgga taacagactt caccaccgca ttctttgaaa
      541 aacgaatggg gatcacctta gatagagaag tacatttatc ccactaacca agtaactgaa
      601 atacagagga atagattatg agcaacaaag cgatttacat tgaaaaaggc aacggtggtt
      661 ggggtggtcc attaacatta cctgttgtgg aaggcaaaaa agtgctttat atgaccggcg
      721 gcactcgccc tgcgattgtt aataaattgg tggaattaac cggctgggaa gcggttgatg
      781 gttttaaaga aggcgaaccg ccggcagaag aaatcgggat tgcgattatt gattgcggtg
      841 gggtattacg ctgtgggctt tacccaaaac gccgtattcc aaccattaac attcactcca
      901 ccggtaaatc cggcccttta gcagaattta ttgtggaaga tatttatgtt tcagcggtga
      961 aagaaaacaa tattcgctta attgatgccg atggaagtgt gcctgtggca acagagcaag
     1021 caaaaccaac ttcatcaacg acagcaacgg caaaagagta tgactccagc aaaaaaatca
     1081 ccgaacaaag tgatggttta ttagcaaaaa tcggtatggg aatgggatcg gtggcggcgt
     1141 tattcttcca agccggacga gacactattg atacggtgct taaaaccatt ttaccgttta
     1201 tggcatttgt ttctgcgtta atcgggatca tcattgcgtc aggtttaggt gatgtgattg
     1261 cccacggctt gacccctctg gcaaacagcc caatcggttt agttacactg gcgttgattt
     1321 gttccttccc tctcctttcc ccattcttag ggccgggagc cgtgattgca caagttatcg
     1381 gcgtgttagt cggtacgcaa atcggtttag gcaatattcc gccacattta gcactgccgg
     1441 cactctttgc tattaatgct caagccgctt gtgattttat tcctgtgggt ctttcaatgg
     1501 cggaagcaaa acaagatacc gtacgggtgg gtgtaccgtc agtattagtc agccgttttt
     1561 taaccggtgc accgaccgtg ctgattgcgt gggcagtttc cgcattcatc taccaataat
     1621 taaccacgat acaagcggtg gaatttgaaa aattttttgc aaaatgcacc gcttgtcaaa
     1681 acgagagaac atttatatga cgattattta tcaaacgacc tttaccaaaa tcgggaattt
     1741 tgctcaggaa tcattaacag acgatatgtt aatcactttt aaacaaggcg caccggcaga
     1801 tttacaagat tactgcttca ttcataatcc atctgaatta agtagccctt tagaagtagg
     1861 cgatatcgct gaatttgatg gcgtagctta tccgattacg gcagttggta gcgttgcatc
     1921 tgaaaactta tccgccctcg gacacattac cttccgtttt gacggggcaa atgatgcgga
     1981 atttccggga agcgttcacg ttatcggaac accgccgcaa ggcttaacag agaactcaac
     2041 cttgattatc aagcgtgatt aatgacggat taaaaataat ttcaaattga aaatttagag
     2101 gaatacatta tgaacaatga aaaaaaagtt gctatcgttg ttggcggcgg tcaaacactt
     2161 ggagcttttt tatctgaagg cttggcagat gccggttatc gtgtggtggt tgctgactta
     2221 aacggcgaaa atgcacaaaa agtggcagcc gacattaatg ccaaacaagg cgaaggaaat
     2281 gcgattgggg tgcaagtcga tgcggctgat gaaccaagcg ttgaagcact agctaaagca
     2341 acagataccg tattcgggcg tgccgatgta ttggtttaca gtgcaggcac agcgaaagca
     2401 tcgcctattt gggatttcaa cttaaacgat tttgatttgt cggtaaaagt gaatttaacc
     2461 ggttatttct taaccgcaaa acacttctcc cgcttaatga ttcgtgacgg tattaaaggg
     2521 cgtatcattc aaattaattc taaatcgggt aaagtaggga gtaagtataa ttccggttat
     2581 agtgcagcga aatttggtgg tgttggttta acacaatcgt tagcattaga cttggcagat
     2641 aaaggcatta ccgtgcattc attaatgtta ggcaatttat tgaaatcgcc gatgttccaa
     2701 tcgttaattc cgcaatatgc ggcaaaactc ggcattccgg aaagcgaagt agagcaagtt
     2761 tatattgata aagtgccttt aaaacgtggc tgtgattatc aagatgtgtt gaatgtctta
     2821 aaattctatg ccggcgaaga ggcagcttat tgcacaggac agtctattaa tattaccggt
     2881 ggtcaggtaa tgttctaagc aaacaagcgg tcaaatttgt cgattttttt acaacaattt
     2941 gcaaaaaatg actaaaataa accgcttgtt aattcgccac accggttaat atgccacagg
     3001 ggaaagcgta ttgttttgaa atcgcaggta cgtttaggct ctgtggcttt ttattaagga
     3061 gcaatgatgg attcaattac acttctgatt atattggcaa tgataatgtg gctgttacaa
     3121 gccgttttgg gttggcttca aattaaagcc tttaaccaag cctttatgga aatcagtaag
     3181 aaaggcaaag taacggttgg gcgtaatagt ggtcgtttta atcctaaatc ggttattgtg
     3241 ttggcgtttg atgatgagaa aaaagtggta gatagccttt gtatgaaagg attttccgtc
     3301 tttgccagac cgcagaaatt agcgtctgtg attggtttgt ctcttgatca aattgtgccg
     3361 gaaaaaatct ttccaagcga taaacgttca caatttgcct taaaagtggc gatatcttcc
     3421 ggatatagtt gctcagatca aaaataatgc gattttttat ttcattttga atattttttt
     3481 agttaaaaat gagataatgg agaataaata tgcagatcaa tagtgtcatc tttgatatgg
     3541 acggggtaat gattgattca gaaccccaat gggcagaggc tcaaattgca gtattagccg
     3601 acttaggtat taatatcacc gagcaagatt gcgaacaact caccagaggt aagcggattg
     3661 atgagttgag cgacacttgg attaaaaaat ttcagttatc tgtttccaac caagcattaa
     3721 gtgaagccat tttggcacac ggttgtgctg cgattgaaca acacggcaaa gcattaacgg
     3781 gtttatatga attattggat ttcttaaaat ctaatcagat caagttagcc gttgcaacct
     3841 catcgccgcc ggtggtgatt aaagcggtgt ttgatcgctt aaaactgtgg gattatttcc
     3901 cgattcgatg cagtgcaatg gacgaagaat atggcaagcc acaccctgcg gtctatttga
     3961 gtgcagtgaa aaaattaggc attgaaaaac aagagtgtat tgttattgag gacagtgtaa
     4021 ccggcttaat tgccgccaaa gccgctaatt taactacatt tttggtgaat accgattatc
     4081 aaaatgaaaa attttcaatt gccgataaaa gaatggcatc gctactagat gtcattgaaa
     4141 aattaaaaca gcattagcac agattagctc gctccaatct ttgctaaatt ggaggaacaa
     4201 tgaaaccttt tgaaagaaga cagcaaattt tagcctattt agctcagcac ggtagaaccg
     4261 aagtggacgt gctggcagac tattttaagc taactggtgc aaccattcgt aaagatttaa
     4321 cttcacttga agaagataag aaagtcttgc gtacttatgg cagtgtagtc ttaattgatg
     4381 aagaaattct tgaacgggaa gagaaaatca ataaagagga caaagacgat aaaccgcttg
     4441 cacagaaaac cggcattaat cttcaacaaa agcaagctat cgggcaaaaa gctgcagagt
     4501 taattaatga gggaaactct attatttttg atgcaggcag cacagtgtta caaatgattc
     4561 cccatttaaa agcatttgat aacctctctt taatgacaaa tagtttaact attttcaatg
     4621 agattttgaa actcaataaa tcctataact tactgatttc cggtggtaca tttcgtgaga
     4681 aatcagcttc atttcacggt tattttgctg aatccgcttt tattggttca aattttgaca
     4741 tactctttat tggcacagac ggactagatt tagacgttgg attaacgact ttcaatgaag
     4801 tatataaagt cagttcgcta atgtgtgatg cggcaaataa aatcgtctta cttgccgact
     4861 ccagtaagtt tggacgtaaa agtccgaata ttgtgtgtgg gttagaaaaa attcatacta
     4921 tcattaccga tgaaggatta tctcaagaaa tgagagaaca gattaaagcg aaagggattg
     4981 agctgttagt tgtataaggg cgtaaaaatg aatcatttag acagtgcaaa agaaacgtta
     5041 aacctctatg ctcagcagat tatcacgcta aaccatcgac tttctgaaga atttaatgag
     5101 gtggtaaata tgatactcgc ttgtcaaggg cgagtggttg ttggcggtat tggaaaatcg
     5161 gggctggttg gcaaaaaaat ggtggcaact tttgcctcca ccggtacacc gagctttttc
     5221 ttgcacccga cagaggcttt tcacggcgat ttgggaatgc tgaaaccgat tgatatggta
     5281 attctgattt ctaacagtgg tgaaactgat gatgtgaata aactgattcc aagcctgaaa
     5341 aatttcggca ataaaattat tgcgatgaca ggtaatcctt actccacact tgggcgaaat
     5401 gccgatgtga ttttaaatat tggtgtagag cgtgaagcct gcctgaataa tctagctcca
     5461 accagctcta ctttggtaac gatggcttta ggcgatgcgt tagccattgc gctaatgaaa
     5521 gcccgtgatt tccgccctga agattttgcc cgctaccacc cgggtggcag tcttggtcgt
     5581 aaattattga acagagtgcg agatgtgatg gtgcgtaaag tgccaaccgc cagcttagac
     5641 accaccttta ccgaatgttt atctgtgatg aacgaagggc gtatgggggt ggcggtaatt
     5701 atgcaagacg ataaattgga aggcattatt accgatggtg atatccgccg cacattagcg
     5761 aaattcgggg cggagagcct aaataaaacc gctgacgaaa ttatgacccg taatccaaaa
     5821 accattaacg ataccgaatt tttagccaaa gccgaagatt taatgaaaga gctgaaaatt
     5881 cactcattaa ttgcagtgaa tgatgagggt aaagtaacgg gattgatgga gttctctagt
     5941 taatttatag atgtaacgat ggcgcaagcg tttacgcttg tgccgttcac tgacatatat
     6001 caatatctaa taaaacaggt aagttgttag cataatttct tgcactgcta tatttccatt
     6061 caaatgcctc gcatacaaaa ccgcttacga tagggttgtt gtgaatatgg tttagctttt
     6121 catcaaaaaa tttctgagaa tagattgtta tcggttggtt atgatgttgc caaagttggt
     6181 attgcttgac attatttcgt tgtttagctg cccgagtaaa catccataat aaccattctt
     6241 ttctactttc ttggggattt gttttaattt gctgaatcaa catttagatg taaatgtttt
     6301 aaaatctctt attaaatctg acgggttttg gtcttttgct tgaaataata aatgaatatg
     6361 gcttggcata atgcagtagc catataaaat
//
LOCUS       DANLXS010000140         6386 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.141, whole genome shotgun sequence.
ACCESSION   DANLXS010000140 DANLXS010000000
VERSION     DANLXS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.141"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            29..280
                     /locus_tag="PXD71_002021"
     CDS             29..280
                     /locus_tag="PXD71_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6292102.1"
                     /translation="MKIVVFQRKALKQLRKIPTGAEIRRKCDDLAYFPYCANIKALTN
                     HQYDYRFRVGNYRIFFNIEGETMNIVSIEEVKKRDERTY"
     gene            264..611
                     /locus_tag="PXD71_002022"
     CDS             264..611
                     /locus_tag="PXD71_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6292103.1"
                     /translation="MKELTNIQYLNNEEGKPAFAVLPIATLEWLKQKANIKTAIETGI
                     PSAVAGIALDNNYSALRAWREYLGLTQEEVAQRLGISQSAYSQHENAKNLRKTTRIKI
                     AYALGLNEEQLDF"
     gene            complement(652..1812)
                     /gene="trmG/rlmN"
                     /locus_tag="PXD71_002023"
     CDS             complement(652..1812)
                     /gene="trmG/rlmN"
                     /locus_tag="PXD71_002023"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDL6292104.1"
                     /translation="MSELITNAGCGSAEQPKEKINLLNLNRQEMRELFAEMGEKPFRA
                     DQLMKWIYHFGEENFDNMSNINKVLREKLKRIAEIKAPEVAVEQRSADGTIKWAMQVG
                     DQQIETVYIPEDDRATLCVSSQVGCALACTFCSTAQQGFNRNLSVAEIIGQVWRASKI
                     IGNFGVTGVRPITNVVMMGMGEPLLNMNNVIPAMEIMLDDFAYGLSKRRVTLSTSGVV
                     PALDKMREQIDVALAISLHAPNDELRDELVPINKKYNIKMLMDSVNKYREVSNANHGK
                     VTIEYVMLDHVNDSTNHAHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQK
                     TLMEYGFTVTVRKTRGDDIDAACGQLAGDVIDRTKRTLEKRKFGQGIAVKTH"
     gene            2072..2968
                     /locus_tag="PXD71_002024"
     CDS             2072..2968
                     /locus_tag="PXD71_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL6292105.1"
                     /translation="MKLSTIKKALVLTFGLSAAMAMAEPFKVVTTFTVIQDIAQNVAG
                     DKATVESITKPGAEIHDYQPTPKDIVKAQKADLVLWNGMNLERWFERFFENVKGKPAV
                     VVTEGITPMPITEGEYKNLPNPHAWMSTDNALIYIENIRAALVKYDPQNADTYNANAK
                     AYAEKVKGIAEPLRQRLAFIPEQQRWLVTSEGAFSYLAKDYNLKELYLWAINAEEQGS
                     PKQVKKVIDGVKANKIPVVFSESTVSDKPAKQVAKETGALYGGVIYVDSLSEKDGPVP
                     TYLDLLKVTIGTIVEGFERSKK"
     gene            2981..3724
                     /locus_tag="PXD71_002025"
     CDS             2981..3724
                     /locus_tag="PXD71_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL6292106.1"
                     /translation="MTTVSASISVENLSVRYNNGHIALQDVSFHLENGTICALIGVNG
                     GGKSTLFKSLMGLVKPLTGSVKLSQLPIAKALKQNLVSYVPQSEEVDWQFPVSVYDVV
                     MMGRYGYMNFLRRPSQTDKLKVEQAMQRLDVLHLKDRQIGELSGGQKKRVFLARALAQ
                     ESKIILLDEPFTGVDVKTENAIVELLKQLKNEGHLILVSTHNLASVPSFCDQVLMVNR
                     TLLAKGKTEEIFNNQNLERVFGGLLHYQK"
     gene            complement(3781..4590)
                     /locus_tag="PXD71_002026"
     CDS             complement(3781..4590)
                     /locus_tag="PXD71_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL6292107.1"
                     /translation="MLNWILEPLQFEFMQIALITAILVAGVSAVLSCYLVLKGWSLMG
                     DAISHAVLPGVVISSLAGLPLSIGAFFSGLGCALSVGYLKENSRLKEDTVMGIVFSGM
                     FAFGILLFTAFNTGEHLTHILFGNLLGVTKSDLLQTLLISLFTFVVMVAKRKDFLLYC
                     FDVNQAKVAGLPVRLLHYGLLSLLALTIISAMQVVGVILVVAMLITPGITAYLLTKRF
                     DSMLLIALGVAIFSSVFGTLLSYHIDAATGPTIILVQAAIFFLSFAYSKIH"
     gene            complement(4583..5443)
                     /locus_tag="PXD71_002027"
     CDS             complement(4583..5443)
                     /locus_tag="PXD71_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL6292108.1"
                     /translation="MLDLLLEPFTYNYMQKAIFVSMGVGLVCAFLSAFLMLKGWSLIG
                     DALSHAVVPGVAIAYALKLPYAFGAFFSGILAALSILWVKSLTKIKEDAVIGFIFTTF
                     FALGMFIVSLNPTSVDVNSIVMGNILGIADDDLWQVVLIIAISLLGLILFWKDLLLVF
                     FDEHHALSVGLSPLRYKVIFFTLLSACVVVALQTVGAILVIAMVVTPGATAYLLTDRF
                     PRLVLIAMLIGTLTSGIGAYLSYFLDGATGAVIVCLQTLIFLFAFCFAPKYGLITQKR
                     KLHRVEVQNA"
     gene            complement(5517..6302)
                     /locus_tag="PXD71_002028"
     CDS             complement(5517..6302)
                     /locus_tag="PXD71_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDL6292109.1"
                     /translation="MMKKITILGLGWLGLPLAEALQNKGWRVAGTKRTVGELAIECYP
                     LDLNALAVTSEIEQLLAADALVIALPLSQIRPENYGCGIQTLVSLAIEKGLKHLVFTS
                     STSVLPMKSGVFDENAVVDSANLLVKMENWLLSQPIHCDILRLAGLVGKKRHPVFYLA
                     GKQNLSGAGQPVNLVHLEDCIAAISLLLEQPNGQRIFHLCAEQHPSRKDYYSEMAKRF
                     GLADLHFSEENQPLVRLIKADKICQELGFRYYYPDPYQFKVDI"
BASE COUNT         2070 a         1375 c         1247 g         1694 t
ORIGIN      
        1 ttataaaact tataaaatag aggcaaaaat gaaaatagtg gtattccagc gtaaagcctt
       61 aaagcaatta cgaaaaatcc caactggtgc agaaattcgc cgaaaatgtg atgatttagc
      121 ttactttccc tactgtgcca atattaaggc attaacaaat catcaatatg attatcgctt
      181 tcgggtcgga aattaccgta tttttttcaa tattgaaggc gaaacgatga atattgtgtc
      241 tatcgaagag gttaaaaaac gagatgaaag aacttactaa tattcaatat ttaaataatg
      301 aagaaggaaa acctgccttt gccgtacttc cgatagcaac acttgaatgg cttaagcaga
      361 aagcaaatat taaaaccgca atagagaccg gtattccatc tgctgttgct ggaattgcat
      421 tagataataa ctactcagct ctgcgtgctt ggcgggaata tttaggttta actcaagaag
      481 aggtggcaca acgcttgggg atttcgcaat cagcttatag ccaacacgaa aatgcaaaaa
      541 atctacgaaa aacgacccgc ataaaaattg cctatgcgtt gggtttaaat gaagagcaat
      601 tagatttcta ataacaaaaa ggcaggaacc tctgcctttt cactattcag attaatgggt
      661 tttaaccgca ataccttgcc cgaatttacg tttttcaagg gtgcgtttgg tgcggtcaat
      721 cacatcgcca gcaagctgtc cgcaagcggc atcaatatca tcacctcgtg ttttacgcac
      781 tgttaccgtg aaaccgtact ccatcaaggt tttttggaaa cgatctaccc gagagttaga
      841 gcttttacca taaggagctt ctgggaacgg gttccacgga atcaagttaa ttttacacgg
      901 cgtgtttttt aatacttcag caagttggtg ggcgtggttt gtgctgtcat tgacatgatc
      961 taacatcaca tattcaatcg tgaccttgcc gtggttggcg ttagaaactt cacggtattt
     1021 attcaccgaa tccatcaaca ttttgatgtt gtacttttta ttaatcggca ctaattcatc
     1081 acgcagttca tcgttcggag cgtgaagtga aattgccaac gccacatcaa tttgctcacg
     1141 cattttgtct aatgccggca ccacacccga agtggaaagc gtgacacgac gtttagataa
     1201 gccgtaagca aaatcatcta acatgatttc cattgccgga atcacgttat tcatattcag
     1261 caatggttca cccattccca tcatgaccac attggtaatc gggcgaacac cggtcacgcc
     1321 aaaattgccg ataattttag aagcacgcca cacttgcccg ataatttccg ccacgcttaa
     1381 gttacggtta aagccttgtt gagcggttga gcagaaggta caagccaagg cacagcctac
     1441 ttgtgaagaa acacacaggg ttgcacggtc atcttcagga atatataccg tctcgatttg
     1501 ctgatcgccc acttgcattg cccatttaat cgtaccgtct gcagaacgtt gttctaccgc
     1561 cacttcaggg gctttaattt cggcaatacg ctttaatttt tcacgcaata ctttattgat
     1621 attgctcata ttatcgaaat tttcttcgcc aaaatggtag atccatttca ttagctggtc
     1681 ggcacggaaa ggtttttccc ccatttctgc aaacagttct cgcatttctt gacgattaag
     1741 atttaataaa ttgatttttt cttttggttg ttcagcagac ccacagcctg cattggtaat
     1801 cagttctgac ataaagttga aagcctcgtt attacacgga ttacggcaaa aggttagggc
     1861 aaatgccctt attttgcgat tggattttaa agcgtgcaat tttactggct cttgagggat
     1921 taggctagta atttttaatc aaaagagttg aattgcctga gtaaacttat caagaataac
     1981 cttataaaaa caagactatt tacatcattc ccaatgttct atagaatagc tttcgataat
     2041 tctcattctc aataacaaaa ggaggctaaa aatgaaacta tcaacaatta aaaaagcatt
     2101 ggtattaacc tttggtttaa gtgcagcgat ggcaatggca gagccgttta aagtagttac
     2161 cacttttacc gtgattcaag atattgcaca aaatgtcgcc ggcgataaag ccactgtgga
     2221 atccattact aaaccgggtg ctgaaattca cgattatcaa ccaacaccaa aagacattgt
     2281 aaaagctcaa aaggcagact tagtgttatg gaacggtatg aaccttgagc gttggtttga
     2341 acgtttcttt gaaaatgtaa aaggtaaacc ggcggttgtc gtcaccgaag gcatcacccc
     2401 aatgccgatt accgaaggtg aatataaaaa tctaccaaat ccgcacgctt ggatgtccac
     2461 cgataacgca ttaatttaca ttgaaaatat tcgtgcggct ttagtaaaat atgacccaca
     2521 aaacgcagac acctataatg ccaatgcaaa agcctatgct gaaaaagtaa aaggtattgc
     2581 tgagccactc cgccaacgtt tagcttttat tcctgagcag caacgttggt tagtaaccag
     2641 tgaaggggca tttagctatt tagcgaaaga ctacaactta aaagaacttt atttatgggc
     2701 aatcaatgcg gaagagcaag gttcgccaaa acaagtgaaa aaagtgattg atggtgtaaa
     2761 agccaacaaa atccctgttg tgtttagcga aagtaccgtt tccgacaaac cggcaaaaca
     2821 agtggcgaaa gaaaccggtg cattatacgg tggcgtaatt tatgtggatt cactttcaga
     2881 gaaagatggt ccggttccaa cctatttaga tttgcttaaa gtgactatcg gcaccattgt
     2941 cgaagggttc gaacgaagca aaaaataata agatttagca atgacaactg tttcagcctc
     3001 catttccgtc gaaaatttat cggtccgtta taataacgga catatcgctt tgcaagatgt
     3061 ctcgtttcat ctggaaaacg gcacaatttg tgcattaatc ggcgtgaatg gaggtggaaa
     3121 atccactctg tttaaaagtt taatggggct ggttaagcca ttaaccggct ctgttaagct
     3181 gagtcagctc ccgattgcta aagccctcaa acaaaatttg gtttcctatg tgccacaaag
     3241 tgaagaagtg gactggcaat ttccagtttc ggtttacgat gtggtaatga tggggcgata
     3301 cggctatatg aattttctac gccgcccaag ccaaaccgat aagctcaaag tagagcaagc
     3361 aatgcaacgc cttgatgttt tacatctcaa agacaggcaa atcggcgaac tttccggcgg
     3421 gcaaaaaaaa cgggtatttt tagcccgtgc cttagctcaa gaaagtaaaa tcatcttact
     3481 tgatgagcct ttcaccggtg tggatgttaa aaccgaaaat gcgattgttg agctacttaa
     3541 acagcttaaa aatgaggggc atttgatttt agtttccacc cacaaccttg ccagcgtacc
     3601 aagcttttgc gatcaggttt tgatggtcaa tcgcacgcta ctggcaaaag gtaaaacgga
     3661 agaaatcttt aataatcaaa atttagaacg ggtgttcggc ggtttattgc attatcaaaa
     3721 gtagtgtttc agcaacaaat cacaaacaaa tagggaggca aatgcctccc tatttttgta
     3781 tcaatgaatc ttagaatatg caaaactgag gaaaaaaatg gccgcttgca cgagaataat
     3841 cgtcggccct gttgcggcat caatatggta gctaagcaac gtgccgaata ccgatgaaaa
     3901 tatcgcaacg cctaaggcaa tcagtaacat actatcaaac cgcttagtca gcaggtaagc
     3961 agtgatcccc ggggtaatca acattgccac cactaaaatc acgcccacca cttgcattgc
     4021 actgataata gttaaagcaa gcaaggaaag taaaccataa tgcaataatc taaccggtaa
     4081 gcctgctact ttggcttgat taacatcaaa gcaatagagc aagaagtctt tgcgtttcgc
     4141 caccatcacc acaaaggtaa agaggcttat gagtaaagtc tgaagcaaat cgcttttggt
     4201 tacgcctaat agattaccaa ataaaatatg ggttaaatgc tcaccggtat taaaggcggt
     4261 aaacagcaag atcccaaagg caaacatacc tgaaaacaca atccccatta ccgtgtcttc
     4321 tttcaagcga ctattttctt ttaaatagcc caccgataag gcacagccta atcctgaaaa
     4381 aaacgcccct attgagagtg gtaacccggc aagtgaggaa atcaccacac cgggcagaac
     4441 agcgtgcgaa attgcatcgc ccattaaaga ccagcctttc agcaccagat agcaagataa
     4501 aaccgcactc acgccagcaa ctaaaattgc ggtaattaac gcaatttgca taaattcaaa
     4561 ttgcaacggc tctaaaatcc aattaagcat tttgaacctc cacacggtgc aatttccgct
     4621 tttgagtaat caagccatat ttaggagcaa agcagaatgc gaataagaaa attaaggttt
     4681 gtaagcacac aattaccgca ccggttgcac cgtctaaaaa gtagcttaaa tatgccccaa
     4741 tccctgatgt cagcgtgccg ataagcattg cgatcagcac taatctaggg aaacggtcgg
     4801 taagtaaata agcagttgct cccggcgtga cgaccatcgc aataaccaaa attgccccaa
     4861 ccgtttgcag agccactact acacaggcac ttaataaggt aaagaaaatc actttatagc
     4921 gtaatggcga aagcccgact gacaatgcgt ggtgttcgtc aaaaaagacg agtagcaaat
     4981 ccttccaaaa taggattaaa cccagtaacg aaatcgcaat aatcagtacc acctgccaca
     5041 agtcatcatc agcaatgcct aaaatatttc ccattacaat cgaattaaca tcaaccgatg
     5101 ttggattgag ggaaacaata aacataccaa gcgcaaaaaa agtcgtgaaa ataaagccga
     5161 taaccgcatc ttctttaatt ttcgtcaggc tttttaccca aagaatcgat aatgccgcca
     5221 aaatcccgga aaaaaacgca ccaaaggcgt agggcaattt tagtgcataa gcgatggcaa
     5281 cacctggcac tacagcgtgt gataaggcat caccaatcaa agaccagcct tttaacatca
     5341 aaaaggcgga aagaaaagca caaactaacc ccacgcccat actgacaaaa atcgcttttt
     5401 gcatatagtt gtaggtaaaa ggctctaata aaagatcaag cataaaatcc taacaagttg
     5461 ttgataatag ttcgcatata ctatcatata aactcctttt cttgtatgga ttaaacttaa
     5521 atatctactt taaattgata aggatcagga tagtaataac gaaaaccaag ctcttggcaa
     5581 attttgtctg cttttatcag ccttacaagc ggttgatttt cttcagaaaa atgcaaatcc
     5641 gctaacccaa aacgttttgc catttcacta taataatctt tacggcttgg gtgctgttcg
     5701 gcacataaat gaaaaatacg ctgcccgttc ggctgctcca acagcaatga aatggctgca
     5761 atacagtcct ctaaatgcac taaattgacc ggctgccctg ctccactcaa gttctgtttt
     5821 cccgccaaat aaaaaaccgg gtgccgtttt ttgcccacca agccagctaa acgcaaaata
     5881 tcacaatgaa ttggctggct cagcaaccaa ttttccattt ttactaatag gttagcagaa
     5941 tcaaccactg cattttcatc aaaaacgccc gatttcattg gcaaaaccga agtcgagctg
     6001 gtgaaaacca gatgtttcaa gcccttttct attgcaagcg aaactaatgt ttgaataccg
     6061 caaccgtaat tttcagggcg aatttggcta aggggcaagg caatcactaa cgcatcagca
     6121 gcaagtaatt gctcgatttc gcttgtcact gctaacgcat ttaaatctag aggataacat
     6181 tctatcgcta attctccgac cgttcgctta gtacctgcaa cacgccagcc cttattttgt
     6241 aatgcttcag ctaaaggtag tccgagccag ccaagcccta aaatggtaat ttttttcatc
     6301 atttacgttt ctcaagatta aaattggaat gaggggaaat tcaggtacaa aaaaagcacc
     6361 tcgtggtgct atctatttta tttcta
//
LOCUS       DANLXS010000141         6275 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.142, whole genome shotgun sequence.
ACCESSION   DANLXS010000141 DANLXS010000000
VERSION     DANLXS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.142"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..272)
                     /locus_tag="PXD71_002029"
     CDS             complement(<1..272)
                     /locus_tag="PXD71_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011497995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6292110.1"
                     /translation="MFYSNNPLIKHKTGLLNLAEELGNISQACKVMGMSRDTSYRYQQ
                     AVEQSGVDALLNQNRRVPNLKNRVDEATEQAVVKFAFDNPAFGQVRV"
     gene            complement(371..601)
                     /locus_tag="PXD71_002030"
     CDS             complement(371..601)
                     /locus_tag="PXD71_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292111.1"
                     /translation="MSLLKKAQTFISIFNDVRTKNEAQRQSSNNLNVALKNAAEMRAE
                     MNGFSLKPQKRSTYFADHYGCGIHAVETYDNV"
     gene            complement(671..1222)
                     /locus_tag="PXD71_002031"
     CDS             complement(671..1222)
                     /locus_tag="PXD71_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292112.1"
                     /translation="MCNQKTLSLYGLVKELGIEKTQLFLEQAFSCSRNKDVESFLLNK
                     AIRFETALAAATHLIINEQGDILAYFTLSFKEIEVEVSKSQLKKLTAGLSNDSHLRVL
                     LIGQIGKNEKVIDNPAKLPDILNHIYAQLYKAQRVLGGRVVILECENTPKLLELYKQN
                     KFQILNTIGSENGLKTLFFIPDF"
     gene            complement(1183..1359)
                     /locus_tag="PXD71_002032"
     CDS             complement(1183..1359)
                     /locus_tag="PXD71_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292113.1"
                     /translation="MATSSFDKRFVVKDPKAVKVLIEALQSPIKVVLPKKDLEKEEKE
                     ALCAIKKRLASMGL"
     gene            complement(1559..1813)
                     /locus_tag="PXD71_002033"
     CDS             complement(1559..1813)
                     /locus_tag="PXD71_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292114.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFCNYQQFMPEVEAKESLR
                     KQEPELDDRYNEEEYSEEEYPDENDASDENDNYDETYNVF"
     gene            2052..2306
                     /locus_tag="PXD71_002034"
     CDS             2052..2306
                     /locus_tag="PXD71_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292115.1"
                     /translation="MTNQQLLAIAQEYRERYQDNHPCMLSSGYVRIYQGEAYGWCAEK
                     GEANTECPEALLVSLTGDIFEAVGGDKLKGAKKWQPYKKE"
     gene            2310..2456
                     /locus_tag="PXD71_002035"
     CDS             2310..2456
                     /locus_tag="PXD71_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292116.1"
                     /translation="MDTTQKRVALFIIGLSVVMWLRKHNCCTVIFVLLLAALAFSYTM
                     EHLY"
     gene            2472..2678
                     /locus_tag="PXD71_002036"
     CDS             2472..2678
                     /locus_tag="PXD71_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292117.1"
                     /translation="MVKNVVHEEILHNRAELQQAVEKEEFCAKCDNSVVFHLRDNYHE
                     FTLGLDTILQCLHFAEEKGAIRIP"
     gene            2675..3451
                     /locus_tag="PXD71_002037"
     CDS             2675..3451
                     /locus_tag="PXD71_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292118.1"
                     /translation="MNIEYSLNHCQGDGVAFYGRLGTDDLIQLFNHLNPQKRKQKMFA
                     NLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMELYAKTADQLVFFNDSDARRNWYF
                     PTSKVYKYKGLWDEFIQDLEQYIKGTSREMESVGYQIIEASPYQTETVFKFNTENYLV
                     ELKRKPSEFYSELVDWAFGDMCDFEATIEQICQGQLCYADFEATVTDKKSEIQLGLAR
                     LSGISYSPNDRSFSGYRQELIGEAIASARHNAARFAYLHA"
     gene            3566..3978
                     /locus_tag="PXD71_002038"
                     /pseudo
     CDS             3566..3978
                     /locus_tag="PXD71_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
     gene            3992..4147
                     /locus_tag="PXD71_002039"
     CDS             3992..4147
                     /locus_tag="PXD71_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292119.1"
                     /translation="METRIVKVIYDDGDYFFTRINADEQAIRNYYAIGSSIGFDDIRK
                     IIAVEIL"
     gene            4227..5201
                     /locus_tag="PXD71_002040"
     CDS             4227..5201
                     /locus_tag="PXD71_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL6292120.1"
                     /translation="MNTQPKFDLYQHITDRIVVALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRPDE
                     REKLDEEGNIVYDEEGNPEMERHFFINKHNLFNLDQCENLPVELYDPVSEPKQPKSEP
                     NQYDIFAEIRQIIKGMDLQVEMKPSNRAFYQPGEDKVVMPEMKQFANAQAFHSVLLHE
                     MTHATGANNRLNREGITSGKAKFGNKVYAFEELIAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVLKEDKRAIVRASGKAREACQYMLDALNVAQLADKYDVFEEAA"
     gene            5288..5485
                     /locus_tag="PXD71_002041"
     CDS             5288..5485
                     /locus_tag="PXD71_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292121.1"
                     /translation="MTKSTEIPRGHDNWVITGRDNDGKWHKERFLYYSRREAIQQFRR
                     KHNCVGKHSVIESITPQFGWY"
     gene            5562..5804
                     /locus_tag="PXD71_002042"
     CDS             5562..5804
                     /locus_tag="PXD71_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292122.1"
                     /translation="MFNLNLFKRPIELSTLESWSKFCDDVGKVAFIAIPPMIYSNYDL
                     CIKIGNISALLAITYGFLLSGRTIRQFIEKEKSRWD"
     gene            complement(5964..6221)
                     /locus_tag="PXD71_002043"
     CDS             complement(5964..6221)
                     /locus_tag="PXD71_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292123.1"
                     /translation="MNNKQILSIAQKFRQNHKDSHPFMLERGYVRIYQGKAYGWCAEQ
                     EDANTECPTALIVSLSNEIFEATGGDDISGAEKWEKYNSNF"
BASE COUNT         1893 a         1107 c         1259 g         2016 t
ORIGIN      
        1 actcgtacct gcccaaatgc cggattatcg aatgcaaact tcacaacagc ttgctctgtt
       61 gcctcatcca cacgattttt tagattagga acacgccgat tttgattaag taaggcatcc
      121 accccacttt gctcaactgc ttgttgatag cgataggatg tatctcgact catccccatc
      181 accttacagg cttgagagat attgcccagt tcttctgcta aatttaataa accggtcttg
      241 tgtttaatga gagggttgtt agaataaaac atgagagttt ccttttttgt ttagattgaa
      301 tttggacact catattctaa acgggaaact ctcacttttt agagtgaatt gtcagatcaa
      361 gtctgatctt ctacacatta tcataggttt ctactgcatg aataccacaa ccataatggt
      421 cagcaaagta agtagaacgc ttttgaggct taagggagaa cccattcatc tcagctctca
      481 tttcagcagc atttttcaag gcaacattta aattgtttga agactggcgt tgagcttcat
      541 ttttagttcg cacgtcatta aaaatagaaa taaacgtttg tgctttttta agtagagaca
      601 ttgttctttc tcccatatat gacttcctga caaagctggc gactttcttc taatgaaata
      661 ttagctgtag ttaaaaatca ggaataaaga aaagtgtttt tagaccattc tcacttccaa
      721 tggtattgag aatttgaaat ttattttgtt tatatagttc taataattta ggcgtatttt
      781 cacattctaa tataacgact cgcccaccta aaactcgttg tgctttataa agttgtgcat
      841 agatatgatt tagaatatct ggcaatttgg ctggattgtc aattactttt tcatttttgc
      901 cgatttgacc gataagcaat actcttaaat gtgaatcatt acttaaacca gcagttagct
      961 tttttaattg gcttttgctt acttccacct caatttcttt aaaagagagg gtaaagtaag
     1021 ccaaaatatc gccctgttca ttaatgatta aatgtgttgc tgctgctaat gcggtttcaa
     1081 atctgattgc tttattaagt agaaaacttt caacatcttt atttctactg caagaaaatg
     1141 cttgctccaa aaagagttgt gttttttcaa tacctagttc ttttacaagc ccatagaggc
     1201 taagcgtttt ttgattgcac atagtgcttc tttctcttct ttttctaagt cttttttagg
     1261 taaaactact ttgattgggc tttgtaatgc ctcaattaat accttgacag cttttggatc
     1321 tttgactaca aagcgtttat caaatgatga tgttgccatt atagaactcc ttaaatattt
     1381 aaattaccat ggttactcat ggctgtaata atactgtatt taggccttgt gggtggtttt
     1441 cgattacata gccttatgct tgttagcata gaaatacttt caattaccac aatatataga
     1501 ctatatttgg aaatgcaagc cttttcaatc acggattcct ccggtttcca tatacatact
     1561 agaaaacatt ataagtttca tcgtagttat cgttttcatc gctggcatca ttttcatcag
     1621 gatattcttc ttcgctatat tcctcttcgt tatatctatc atcaagttct ggttcttgtt
     1681 tccttagtga ttctttagct tcaacttcag gcataaattg ctggtaattg cagaaaaaag
     1741 tgccatcgct gcctttttcc caaagtccgg cgtagttgta ttttacatat tcaggatcag
     1801 cgtggcatat catccctaat tgaggggtgg cataagtgag cgacatcgaa atgctactaa
     1861 ggataagcgt gaggagtttt ggtttcacag caaaataggg ttaggtaaat ttacttcagt
     1921 gtatggaaaa acataggaat gttgagcaga aactaaaaat gatttgaata tagtttttgg
     1981 tttctattta taaaaatgtt gccagaatac taagaacgtt ttcaggttta ttatgttatt
     2041 ggagacttta tatgactaat cagcaattac tagctattgc tcaagagtat cgtgagcgtt
     2101 atcaagataa ccatccctgt atgttgagta gcggctatgt tcgtatttac caaggtgagg
     2161 cttatggttg gtgtgctgaa aaaggagagg caaatacaga atgccctgag gccttattgg
     2221 tgagtttaac cggcgatatt tttgaagcag tcggcggaga taagctgaaa ggtgccaaaa
     2281 aatggcagcc ttacaagaaa gaatgacaga tggatacgac acaaaaacgt gtggctttat
     2341 tcatcattgg attatctgtt gtgatgtggc taagaaagca taactgctgt acagtgatat
     2401 ttgtattgtt gttggcagcg ttagcattta gctacacgat ggaacattta tattagctgg
     2461 ggaactgaat tatggtaaag aatgtggttc atgaagagat attacataac cgtgcagaat
     2521 tacagcaagc ggttgaaaaa gaagaatttt gtgcaaaatg tgataactca gtagtattcc
     2581 atttacgtga taattatcac gaatttacgc ttgggttaga tactatattg cagtgtctgc
     2641 attttgctga agagaaggga gctatccgga taccttgaat attgagtatt cactcaatca
     2701 ttgccaagga gatggagtcg cattttatgg tcgacttggt acagatgatc taatccagct
     2761 tttcaatcat ctcaatccac agaaacgtaa acagaaaatg tttgctaatt tgctaacgca
     2821 cattgagtct tgggaaaccc acgatgatat tggttttgaa attatacgaa atcgctttgg
     2881 gtatcgttac tcgcattgga atacgatgga gctttatgct aaaactgcag atcagttagt
     2941 gtttttcaat gatagtgatg ctcgaagaaa ctggtatttt ccgacttcaa aagtgtataa
     3001 atacaaaggc ttgtgggatg agtttattca ggatttagaa cagtatatta agggtacttc
     3061 ccgtgaaatg gaatcggtcg gttatcaaat tattgaagcc tcgccgtatc aaacggaaac
     3121 cgttttcaaa tttaatacgg aaaactatct agtagaactg aaacgtaaac cttctgagtt
     3181 ttattcagag cttgttgatt gggcatttgg tgatatgtgt gattttgaag caactataga
     3241 gcagatttgt caagggcaac tctgttatgc tgattttgaa gcaactgtta ccgataagaa
     3301 aagtgaaatt cagttaggtc ttgctcgctt gagtggaata agttactcgc cgaatgaccg
     3361 ttcgtttagt ggttatcgac aagagcttat tggtgaggcg attgcctctg ctcgccataa
     3421 tgcagcacgt tttgcctact tgcacgccta atttgcagat ttgtacttca atttgaccgc
     3481 ttgtagtggt taattttcac cccgtgggga aatattttcc ctattgggca agatgtttct
     3541 ccacttttta atttggagaa atgtgatgaa aaatgaacac aatttagagc aacttaaata
     3601 tatttgggat aaccgcaacc ttgaaaataa agcctatcaa tacctgtttc acatttgcat
     3661 tgagccgatg aaaaaaggag attggaatcg ttatacgatt tggcttaatt ttcacggcaa
     3721 cttaatcaac gtgtcagacg ttgtatatcg agttgtggaa tctcaaaagc tcaaggatga
     3781 catcaaaatg tggtatgaca ataataccat tgtcgtccga taaaagttga aacaacaggc
     3841 ttcatctcat atttactgca ccagttctat caggggatag cctatatttt actggaacag
     3901 ggaatgatta ctgaaactga agcagccgag aaaagttata acttcagagc tgcaaattgt
     3961 tttcaacgtt tgctttaatg gaggtgagat aatggaaact cgcattgtta aagttattta
     4021 cgatgatggt gattatttct ttacccgaat caatgctgat gaacaggcta tccgtaatta
     4081 ctacgccata ggcagttcaa taggttttga tgatataaga aaaattatcg ctgttgaaat
     4141 tttataaacc acccataggg gaaactattt ccccataggg tgatagcgtt tccccatttt
     4201 tctacccgaa ctttggagaa acacttatga acactcaacc taaatttgat ttataccaac
     4261 acatcactga ccgaattgtt gtagcactgg aacaaggtac agccccgtgg ttaaaaccgt
     4321 ggagccatcc ggaatgcaat atggcattac ctcgcaatgc ggtatctaac cgcttgtatt
     4381 ccggtatcaa tacactctta ctctggattg ctactgctga acatggttac aaacagtgca
     4441 agtggattac agcaaaaaaa gccaatgaac tcggcggata tatccgtaaa ggggaaaaag
     4501 ctactgtggt gatgaactac cgtcctgatg agagagagaa actcgatgag gaagggaata
     4561 ttgtgtatga cgaggaagga aatccggaaa tggaacgaca ctttttcatt aataagcaca
     4621 atctgttcaa tctggaccag tgtgaaaatt taccggtgga actgtacgat cctgtatcag
     4681 aaccgaaaca acctaaatct gagccaaacc aatacgatat ttttgctgag attcggcaga
     4741 ttatcaaagg aatggatttg caggtggaaa tgaagcctag caatcgggca ttttatcaac
     4801 caggtgaaga taaggtcgta atgccggaga tgaagcagtt tgcaaatgct caagcgtttc
     4861 atagtgtatt gttacacgaa atgactcacg caacgggggc aaataatcgg cttaatcggg
     4921 aaggtattac ttccggaaag gctaaatttg gaaacaaagt ctatgctttt gaggagttga
     4981 ttgctgaaat gggcggtgca tttttatgtg cctaccttgg ttttgatact gtgcctcaaa
     5041 atgcggcgta tattgaaagt tggattcgag tattgaaaga agataaacgg gcgattgtcc
     5101 gtgcttccgg aaaagctcgt gaagcctgcc aatacatgtt agacgcatta aacgtcgcac
     5161 agttggctga caaatacgat gtatttgaag aagctgctta acaaatttgc cgaaaggcat
     5221 atctcaaggg gagacctatc cctttgggca tagtgtttcc ctatttttta ttaaatggag
     5281 aaaagctatg acaaaatcaa ctgaaatacc tcgtggacat gataactggg ttattacagg
     5341 cagagacaat gatgggaaat ggcataaaga acgttttctt tattattctc gccgtgaagc
     5401 catccaacag tttcgtcgaa aacacaactg cgtaggtaaa catagtgtta ttgaatcaat
     5461 aacacctcaa tttggctggt attaaccggc tttcccgtgg ggagatactc tcccttcgga
     5521 gcattgtgtc tcccttttta ttcacttaac tagaggacac aatgttcaat ctaaacttat
     5581 ttaaacgccc tattgagctt tcaacgcttg aatcttggtc taaattctgt gatgatgtcg
     5641 gtaaagttgc atttattgcg atacctccga tgatttatag taattatgat ctatgtatca
     5701 aaataggaaa cataagtgct ttattggcaa ttacctatgg attcttatta tcaggcagga
     5761 ctatccgtca atttattgag aaggagaaat caagatggga ttaacaatcg gcttatggat
     5821 cattgctttt ataggctttg cactggtttt ttgggcaaat agcttactca aagagcaaga
     5881 tgaacgcctt aaacagctaa aaaaatagct caactttaca ctcaatacgg cacatttggt
     5941 gccgtatttc attcttatcc taattaaaaa ttagaattat atttttccca tttttcagcc
     6001 ccacttatat catcacctcc agttgcctca aaaatttcat tagataagct gacaattaag
     6061 gcggtagggc attccgtgtt tgcatcttct tgctctgcac accagccata ggcttttccc
     6121 tgataaatac gaacataacc acgctctagc ataaaagggt gagaatcctt gtgattttga
     6181 cgaaattttt gagcaataga caaaatttgt ttattgttca taattatttt tcttattgct
     6241 tatatgctga aaattgatga ttgaatttat tatat
//
LOCUS       DANLXS010000142         6216 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.143, whole genome shotgun sequence.
ACCESSION   DANLXS010000142 DANLXS010000000
VERSION     DANLXS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.143"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(29..427)
                     /gene="mutT"
                     /locus_tag="PXD71_002044"
     CDS             complement(29..427)
                     /gene="mutT"
                     /locus_tag="PXD71_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDL6292124.1"
                     /translation="MSKPIIQVSAGIIRNEFGQIYLTQRLEGQDFAQSLEFPGGKVDP
                     NETPEEALKRELEEEIGIQVLSAFPYENFCFEYPTKIIEFFFYLVEEWVNEPYGREGQ
                     EGFWIAQSDLDEGAFPPANAQLIKRLKEHA"
     gene            complement(514..3240)
                     /gene="secA"
                     /locus_tag="PXD71_002045"
     CDS             complement(514..3240)
                     /gene="secA"
                     /locus_tag="PXD71_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL6292125.1"
                     /translation="MISKLLTSIFGSSNDRTLKRLRKRVAQINKLEPTFEQLSDEALK
                     DKTAEFKQRLADGVSLDSLLPEAFATVREASKRVLGMRPFDVQLIGGMVLTGRNIAEM
                     RTGEGKTLTATLSCYLNALTGKGVHVVTVNDYLARRDAETNRPLFEFLGLTVGVNIPG
                     LPSDAKRAAYQADITYSTNSELGFDYLRDNLAHDKNERFQRELHYALVDEVDSILIDE
                     ARTPLIISGPAEDATQIYQAVDQIIPHLIAQDKEDSEEYTGEGDFTLDLKNKQAHLTE
                     RGMVKVEDFLTQMGLMEEGESLYHPARISLLHHVSAALRAHKLFERNVDYIVKDGEIV
                     IIDEHTGRTMAGRRWSDGLHQAIEAKERVAIQGENQTVASITYQNYFRLYEKLAGMTG
                     TADTEAFEFQQIYGLNTVVIPTNRPVIRDDRTDLMFKSEAEKFAAIITEIRDCIERKQ
                     PVLVGTASVEKSELLSAELTKAGIAHNVLNAKFHAQEAEIVAEAGAPGAVTIATNMAG
                     RGTDIVLGGNWKMEVAKLENPTQEQIDAIKAAWQERYDIVMQAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLDDALMRIYLNEGKLNMMRKAFTEEGEAMESKLLT
                     KVIASAQAKVEAHNFDGRKHLLQYDDVANEQRKVIYEQRNHLLETDNIASMIESIRQD
                     VFESTINQYIPPQSIEEMWNVPGLEERLRREFGLDLPISHWLETEKDLHEETLRERIL
                     NIAIAEYNAKEEIVGAEVMRNFEKGVMLQNLDELWKEHLASMDYLRKGIHLRGYAQKD
                     PKQEYKKESFAMFTNMLDTLKLNVISILSRIQVRSKEEVEEAQRQQAEMDAASTPVTQ
                     ATDSAAGSREENSAESLSEEELANLNIGRNDPCPCGSGKKYKHCHGNKARYS"
     gene            complement(3299..3619)
                     /locus_tag="PXD71_002046"
     CDS             complement(3299..3619)
                     /locus_tag="PXD71_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2547 family protein"
                     /protein_id="HDL6292126.1"
                     /translation="MSLFLKHFHRTPFWSQLFLGMVAILALPEIQVAYANESEQETVL
                     NQSFLQYAQSANDIEQQSLFIAELIHKIVEIKPQAVHFCEFFANSYRLDRQNAYPIRA
                     GPQT"
     gene            3740..4042
                     /locus_tag="PXD71_002047"
     CDS             3740..4042
                     /locus_tag="PXD71_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HDL6292127.1"
                     /translation="MKNSAPKKINDILQNSSLSRIVEKANFINDLNQKIQKRWPESYR
                     GLYRVVNLVDNQLIVSVKNATVRQGLILQQNMLLKQIQTDLPEITQLEFRVNPSVT"
     gene            4067..4441
                     /locus_tag="PXD71_002048"
     CDS             4067..4441
                     /locus_tag="PXD71_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HDL6292128.1"
                     /translation="MKKIALIGFTTAMLGACSLLPQKSVSGTYAGELPCADCEKIQAQ
                     LTLNADKTYQYDTVYFKDKKEYTYRDKGTYSWEPNKPNVIRLEKASGNLAFQVSEQHV
                     ELCDPNGNTVKNSNNYKLGKVR"
     gene            complement(4617..4706)
                     /locus_tag="PXD71_002049"
     tRNA            complement(4617..4706)
                     /locus_tag="PXD71_002049"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4670..4672),aa:Ser,seq:gga)
     gene            complement(4798..5109)
                     /locus_tag="PXD71_002050"
     CDS             complement(4798..5109)
                     /locus_tag="PXD71_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL6292129.1"
                     /translation="MSVEQTIIQKIKTEFQPIFFNVENESYMHSSGRGAESHFKLTLV
                     SDKFEGQRTVARHRAVYACLAEELANGVHALALHLYTQGEWEATGGLVPKSLNCLGVG
                     Q"
     gene            5201..5788
                     /locus_tag="PXD71_002051"
     CDS             5201..5788
                     /locus_tag="PXD71_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292130.1"
                     /translation="MKRSKKTLVLATTFVAALLAGCQSQSNTLTFSTPTPTAVFNTNN
                     QTAAVNVMTQDLRTSREIASYTKNGEVIRLTAVPEVAAMFQQAVQQDLNAKGFQIVQG
                     AANANVVVNIRKFFATVEQGNLRYKADAQVSVKVAVQGSKGNFSKNFNATRGYEGAFG
                     ASNDEIKKVLDQAYSDVVKAIYNDNEIANAIHQFK"
     gene            5960..6133
                     /locus_tag="PXD71_002052"
     CDS             5960..6133
                     /locus_tag="PXD71_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292131.1"
                     /translation="MNNPYHNVSQEYLDYLAKKVKQGKEDIKNGRIYSPEEVRKRVIA
                     AAFRGAENAKKVA"
BASE COUNT         1914 a         1321 c         1137 g         1844 t
ORIGIN      
        1 ttactttaga tacagttctc aatacaaatt atgcgtgttc ttttaagcgt ttaattaatt
       61 gggcatttgc cggtgggaaa gctccctcat ctaaatccga ttgtgcgatc caaaaacctt
      121 cttgcccttc tcttccgtaa ggttcattta cccactcttc caccaaataa aagaaaaatt
      181 caatgatttt tgttggatat tcaaagcaaa aattttcata cggaaaagcg cttaacactt
      241 gaataccaat ttcttcttct aattctcgtt ttagtgcttc ttcaggggtt tcattgggat
      301 cgactttccc cccgggaaat tcaagagact gagcaaaatc ttgcccttct aaacgttggg
      361 ttaagtaaat ttgcccaaat tcgttacgaa taatccctgc tgagacttga ataatcggtt
      421 ttgacatagt ttccttcact ctttctataa attcaagcgg tcatattaag cagaaaattt
      481 gcaaattcct gactgaatat gaccgcttgt taattacgaa tatctcgctt tgttaccatg
      541 gcaatgctta tattttttgc ccgaaccaca agggcaaggg tcatttcgac caatatttaa
      601 gtttgccaac tcttcttcac ttaagctctc tgcgctattt tcttctctac taccagcagc
      661 agaatcggta gcttgtgtaa ctggcgtact tgcagcgtcc atttcagctt gctggcgttg
      721 cgcttcttcc acctcttctt tgctacgcac ttgaatacgg cttaaaatgc taattacatt
      781 caattttaag gtatcaagca tattggtaaa catcgcaaaa gattcctttt tatactcttg
      841 cttcggatct ttttgtgcat aaccacgcaa gtgaatacct ttgcgtaaat aatccattga
      901 ggctagatgc tctttccaaa gctcatctaa gttttgtagc attacacctt tttcaaaatt
      961 acgcattacc tctgcgccta ctatttcttc cttcgcattg tactctgcaa tcgcaatatt
     1021 taagatacgt tcacgcaaag tctcttcgtg caagtctttt tcagtttcaa gccagtgaga
     1081 aatcggtaaa tccaacccaa attcacggcg taaacgctct tctaagcccg gcacattcca
     1141 catttcctcg atagattgag gcggaatata ttggttaatc gtgctttcaa atacatcttg
     1201 gcgaatgctt tcgatcattg aggcaatatt atctgtttct aatagatgat tgcgttgttc
     1261 gtaaattact ttacgctgtt cattggcaac atcatcatat tgtaataaat gcttacgccc
     1321 atcaaagtta tgcgcttcta ctttcgcctg tgcagaggca atcactttgg ttaacagttt
     1381 agactccatc gcttcacctt cttcggtaaa ggctttacgc atcatattta atttaccttc
     1441 attgaggtaa attctcatta gggcatcatc taaagagaga tagaaacgtg atgaccccgg
     1501 gtcaccttga cgaccagaac gacctcgcaa ctggttgtcg atacggcggg attcgtggcg
     1561 ttctgtacca ataatatgta aaccaccggc ttgcattacg atgtcataac gctcttgcca
     1621 cgcagcttta atcgcatcaa tttgttcttg tgtcgggttc tcaagcttgg ctacttccat
     1681 tttccagtta ccgccaagca caatatccgt accacgacca gccatattgg ttgcgatagt
     1741 aaccgccccc ggagctcccg cttccgctac aatttcagcc tcttgagcgt ggaatttcgc
     1801 atttaatacg ttatgagcaa taccggcttt ggttaattct gccgataata actctgattt
     1861 ttccaccgat gctgtgccaa ccaaaacagg ctgtttacgc tcgatacagt ctctaatttc
     1921 agtaataatc gccgcaaatt tctccgcttc gcttttaaac attaagtctg tacggtcatc
     1981 acggatcacc ggtctgttgg ttggaatcac caccgtattt aagccataaa tttgctggaa
     2041 ttcaaaggct tcggtgtctg ccgtaccagt cataccggct aatttttcgt acaaacggaa
     2101 atagttttgg taagtaattg aggcaacagt ttggttttcg ccctgaatag ccacacgctc
     2161 tttagcttca atcgcttggt gtaagccgtc agaccaacga cgtccagcca ttgtacgacc
     2221 ggtgtgctca tcaataatca caatttcacc gtcttttaca atgtaatcta cgttgcgttc
     2281 aaataattta tgggcacgca atgctgcact cacatggtgt aacagcgaaa tacgagcagg
     2341 gtgatataaa ctttcgccct cttccattaa ccccatttga gttaagaaat cctctacttt
     2401 caccatacca cgctcggtta aatgagcctg tttgtttttc agatctaagg taaaatcgcc
     2461 ctcaccggtg tactcttcac tgtcttcttt atcttgagca atcaaatgcg gaatgatttg
     2521 atctaccgct tgataaattt gggtcgcatc ttccgcaggc cctgaaataa ttaacggcgt
     2581 acgagcttca tcaattaaaa tagagtctac ttcatcaact aatgcgtagt gtaactcacg
     2641 ttggaaacgt tcgtttttat cgtgagctaa gttatcacgc aagtaatcaa aacctaattc
     2701 gctgttagtc gaataggtaa tatctgcttg gtatgcagca cgttttgcat cggaaggtaa
     2761 ccccggaata ttcactccaa cggttaagcc taaaaactca aataacggac ggttggtttc
     2821 cgcatcacgg cgggcgaggt aatcattcac cgtaacaaca tgaacacctt taccggtcaa
     2881 agcattcaaa taacaagata aagttgcagt tagggtttta ccttccccag tacgcatttc
     2941 tgcaatatta cgccccgtta gcaccatacc gccgatcagc tgtacatcaa atggtcgcat
     3001 acctaatact cgtttgcttg cttctctcac tgtcgcaaaa gcttcaggta ataggctatc
     3061 tagcgataca ccatctgcaa ggcgttgctt aaactccgct gttttatcct ttaacgcctc
     3121 atcagataat tgctcgaagg ttggttcaag cttattaatt tgtgccactc gctttcttaa
     3181 acgttttaaa gtgcgatcgt tgcttgagcc aaaaatagaa gtcagtaatt ttgaaatcat
     3241 tgtgttattc ctaaatataa aaatgggtta atcaattaat ggtaaaatta aattatattt
     3301 aggtttgtgg cccggctcga atggggtaag cattttgtct atccaagcgg taggagtttg
     3361 caaaaaattc gcaaaaatgc accgcttgtg gtttaatttc cacaatttta tggattaact
     3421 ctgcaataaa aagcgattgt tgctcaatat cattagcgga ttgagcatat tgaagaaaag
     3481 attgattaag aacggtttcc tgttcacttt cattggcgta cgcaacctga atttcaggta
     3541 aagcaagtat cgcaaccatt cccaaaaaga gttgcgacca aaaaggcgtt cgatgaaagt
     3601 gttttaaaaa taagctcatt aaattaagat aaatgaaaaa attgggctaa ttatacgcta
     3661 aagtaaagcc gaatgagtaa attattgggt caagtatcgt aaaatcaagc ctaaaataag
     3721 acgaaatgta aagaaacaga tgaaaaattc agcacctaaa aagattaatg acattttaca
     3781 gaactcaagc ttaagccgta ttgtcgaaaa agcaaacttc attaacgatt taaaccaaaa
     3841 aattcagaaa agatggccag aatcttatcg tgggctttac cgagtagtga atttagttga
     3901 taatcagctt attgtgagcg ttaagaatgc aaccgtacgg caaggtttaa tcttgcaaca
     3961 aaatatgtta ctaaaacaga tacaaaccga tttacctgaa ataacccaac ttgaatttag
     4021 agtaaatccg agtgttacat aatttaattt tgaagaggaa aatagtatga aaaaaatcgc
     4081 actcattggt tttaccaccg caatgctagg ggcttgctca ttgctaccac aaaaaagcgt
     4141 tagcggtact tatgcaggtg aattaccgtg tgctgactgt gagaaaatcc aagcccagct
     4201 aaccttaaat gcagataaaa cctaccaata tgatacggta tattttaaag ataaaaaaga
     4261 atacacttac cgagacaaag gcacatacag ttgggaaccc aataagccaa atgtgattcg
     4321 tctggaaaaa gcatcaggca atcttgcctt ccaagtgtct gaacaacacg ttgaactttg
     4381 cgacccaaat ggcaacacgg taaaaaacag caataactat aaactaggta aagtacgtta
     4441 aaagcagata aaaaggattc ttaggaatcc tttttctttg taatctatcg ccgatcgcat
     4501 aaaaaaaccc ccaagcaatg tttgctcggg gataacctaa aaatgaaaaa gtattatcta
     4561 ctttaactta accaaggaaa tatctatgaa aacagtaagg aatctttcag tatgaatggc
     4621 ggtgagaggg ggattcgaac ccccgataca gtttcccata tacacgcttt ccaggcgtgc
     4681 tccttcaacc actcggacat ctcaccgaat caacaaaaag atggcgtaaa tatagcaatt
     4741 ttacacggct ctttcaagta ttttcttcac aaaataaaat aaatcatttt tatccactta
     4801 ttgcccaaca cctaagcaat ttagcgattt aggtacaagt ccgccagtcg cttcccattc
     4861 accttgggta tataggtgca aagcaagtgc gtgaacgcca ttagctaatt cctcagctaa
     4921 gcaagcatag acagcacgat gacgtgcaac tgttctctgc ccttcaaatt tatctgacac
     4981 caaagttaat ttaaaatgcg attctgctcc tctacctgag ctgtgcatat agctttcgtt
     5041 ttcgacatta aagaaaatcg gctgaaattc tgtcttaatt ttttgaataa ttgtctgttc
     5101 taccgacatt ttttgctctc gttcattgaa aatcaaaacg aaataatact atactaagca
     5161 gaaattttct cgaaatttat tcaatataga aggatttact atgaaacgct ctaaaaaaac
     5221 gttagtgtta gccactactt ttgttgccgc attactagcc ggctgccaaa gccaatccaa
     5281 cacattaaca ttctctaccc caacgccaac tgctgtattt aacacaaaca atcaaacggc
     5341 tgcggtgaat gtaatgacac aagatttacg cacctcgcgt gaaatcgcaa gctacactaa
     5401 aaacggagag gtcattcgtt taaccgcagt gcctgaagtt gctgcaatgt tccaacaagc
     5461 cgtccaacaa gacctgaatg caaaaggttt ccaaattgta caaggtgcag cgaatgccaa
     5521 tgtggtagtg aatattcgta aattctttgc cacagtagaa caaggaaact tacgctacaa
     5581 agccgatgca caagtgagtg taaaagtggc agtgcaaggt tctaaaggaa actttagcaa
     5641 aaacttcaac gctacccgtg gttacgaagg tgcatttggt gcaagcaatg atgaaatcaa
     5701 gaaagtgtta gaccaagcct attctgatgt ggtgaaagct atttacaacg ataatgaaat
     5761 cgccaatgca attcaccagt tcaaataatt aattttttct ggcaagcggt catctttctt
     5821 cgattttttg caaaaaacgt ggaaaaatga ccgcttgttg ttttctcgtt ttgtgatgtg
     5881 gctcgaaaaa taagataaat taacttgatt aaacgatcag tagcccccac aatagagact
     5941 catcgcaggg aggaaaaata tgaataatcc ttatcataat gttagccaag aatatcttga
     6001 ttatcttgct aaaaaagtaa agcaaggaaa ggaagatatt aaaaacggtc ggatttattc
     6061 acccgaagag gtcagaaaaa gagttattgc tgccgctttt agaggtgctg aaaatgccaa
     6121 aaaagttgca taatatttat tatactgaag aggccttaat agatttagag aatattgcca
     6181 tctcagttag tgaatttacc ggttatgcaa gtagtg
//
LOCUS       DANLXS010000143         6138 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.144, whole genome shotgun sequence.
ACCESSION   DANLXS010000143 DANLXS010000000
VERSION     DANLXS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.144"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            286..1644
                     /locus_tag="PXD71_002053"
     CDS             286..1644
                     /locus_tag="PXD71_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HDL6292132.1"
                     /translation="MITIKKGLDLPIAGKPEQVIHNGNTVTEVAMLGEEYVGMRPSMK
                     VREGDVVKKGQVLFEDKKNPGVVFTAPASGTVTAINRGEKRVLQSVVIKVEGDEQITF
                     ARYEASQLASLTSEQVKQNLVESGLWTAFRTRPFSKVPALDAEPSSIFVNAMDTNPLA
                     ANPEVIIAEHQQAFFDGLTVLSRLFESKKVIHLCGSADSNILNAAQSRQINNVETNRF
                     KGPHPAGLSGTHIHFIDPVGATKQVWYLNYQDVIAIGKLFTTGELFTDRVVALAGPQV
                     KNPRLVRTRLGANLSQLTANELNAGENRVISGSVLSGAIATGVHDYLGRYALQVSALL
                     EGREKAFFGMIAPYSDKYSITRTTLGHFAKKLFNFTTAVHGSERAMVPTGAYERVMPL
                     DIIPTLLLRDLAAGDTDSAQALGCLELDEEDLALCTFVCSGKNNWGPLLRQALDKIEK
                     DG"
     gene            1647..2882
                     /locus_tag="PXD71_002054"
     CDS             1647..2882
                     /locus_tag="PXD71_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HDL6292133.1"
                     /translation="MGLKNLFEKMEPAFHKGGKYEKWYTLFEAAYTIFYTPGTVTRKD
                     AHVRDAIDSKRMMLIVWLALFPAMFYGMYNVGAQGILAALKMGTLTDLVANNWHYSLA
                     DSLGSVADAGWGTKMLLGATYFLPIYLTVFAVGGFWEVVFAMVRKHEINEGFFVTSIL
                     LALIVPPTLPLWQAALATTFGVVVAKEVFGGVGKNFMNPALAGRAFLFFAYPGQISGD
                     AVWVAADGFSGATALSQWAVGGQGALQHVATGETITWMDAFLGNIPGSIGETSTLMLI
                     IGAAIIVFARIASWRIIAGVMIGMAATATLFNVIGSDTNPLFSMPWHWHLVLGGFAIG
                     MFFMATDPVSAAFTNKGKWAYGILIGFMCVLIRVVNPAYPEGMMLAILFANLFAPIFD
                     YLVVQGNIKRRLARVANNG"
     gene            2875..3648
                     /locus_tag="PXD71_002055"
     CDS             2875..3648
                     /locus_tag="PXD71_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDL6292134.1"
                     /translation="MAKFNKDSVGGTLTVVVLLSLICSLIVAGAAVMLKPTQDIQKQL
                     DKQKNILQAAGLLQKDTNVQETYTKFIEPKIVDLATGDYVEGVTGFDARAAAKDPAQN
                     VVIAPEDDKANIKTRAKYAEVYLVKDESGKTTQVVLPMHGNGLWSIMYGFVAVQPDAN
                     TVNGITYYEQGETAGLGGEIANPNWQKNFVGKKLYNEQNQVALRVGKGASADKDHGVD
                     GLSGATLTSNGVDGSFKYWFGQNGFGPYLAKFKAAAGAN"
     gene            3648..4274
                     /locus_tag="PXD71_002056"
     CDS             3648..4274
                     /locus_tag="PXD71_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HDL6292135.1"
                     /translation="MADNNNLKKLLLSPIVDNNPIALQILGICSALAVTTQLQTAVVM
                     AIAVSLVTAFSSMFISMIRNYIPNSIRIIVQMAIIASLVILVDQILRAYAYDLSKQLS
                     VFVGLIITNCIVMGRAEAFAMKSGPVESFVDGIGNGLGYGAMLIIVAFLRELIGSGKI
                     FGITILETVQNGGWYQANGLFLLAPSAFFIIGFVIWAIRTWKPEQVEK"
     gene            4276..4872
                     /gene="nqrE"
                     /locus_tag="PXD71_002057"
     CDS             4276..4872
                     /gene="nqrE"
                     /locus_tag="PXD71_002057"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005529392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HDL6292136.1"
                     /translation="MEHLLSIFVKSIFIENMALSFFLGMCTFLAVSKKVSTAFGLGIA
                     VIVVLGIAVPANQLVYTHVLKEGALVEGVDLSFLNFITFIGVIAALVQILEMILDKFF
                     PALYSALGIFLPLITVNCAIFGGVSFMVQREYNFVESVVYGVGAGTGWMLAIVALAGL
                     TEKMKYSDVPAGLRGLGITFITVGLMALGFMSFSGIQL"
     gene            4883..6112
                     /gene="nqrF"
                     /locus_tag="PXD71_002058"
     CDS             4883..6112
                     /gene="nqrF"
                     /locus_tag="PXD71_002058"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HDL6292137.1"
                     /translation="MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDP
                     EKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKK
                     EAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
                     VPFRAGGYIQIEAEPHTVHYKDFDIPKEYHEDWDKFDLWRYTSKVDEHIIRAYSMASY
                     PEEKGIIMLNVRIATPPPRNPDVPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAE
                     MVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSEREMFYVEDFDGLQAENDNF
                     KWYVALSDPLPGDREDYFRGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGML
                     KSLGVEDENILLDDFGG"
BASE COUNT         1751 a         1101 c         1417 g         1869 t
ORIGIN      
        1 gaattgaaat cttgatttat ttcaaatttt accgcgggaa tatgcaaaaa atagggggtt
       61 ttcctagcca gaaggggagc tttttaggta aaataagaag attattttta gttaaccttt
      121 tgttaagaaa agataaaggt tgatcacaga tttgagaact taatgtggct tttaaaaata
      181 ggtgggggac cattgtttat ttttaagtaa agctgcggct caatagaagc gattgcgtta
      241 gcgcaatcat attttttaac ccaaaagaaa agtagtgcaa acaatatgat tacaatcaaa
      301 aaaggcttag atcttccgat tgcgggaaaa cctgagcaag taatccataa cggtaatacc
      361 gttactgaag ttgctatgct tggtgaagaa tatgtgggta tgcgtccttc aatgaaagtg
      421 cgtgaaggcg atgtagtaaa gaaaggtcag gttctttttg aagataaaaa gaatccgggc
      481 gtggtattta ctgcccctgc aagtggtacc gtaacggcaa ttaaccgtgg cgagaagcgt
      541 gttcttcaat cggtagtaat taaagttgag ggtgatgagc aaattacctt tgctcgttat
      601 gaagccagcc aattagcttc attaacttca gagcaagtaa aacaaaacct tgtagagtca
      661 ggcttatgga cggctttccg tacacgtccg tttagcaaag tgcctgcatt agatgcagaa
      721 ccttcctcta tttttgtcaa tgcgatggat accaatccac ttgcagcaaa tccggaagtg
      781 attattgcag aacaccaaca agctttcttt gatggcttaa ctgtattaag tcgtttattt
      841 gaaagtaaaa aagtgattca tctctgtggc agtgcagact caaatatttt aaatgcggca
      901 caaagccgtc agatcaataa tgtggaaact aaccgcttta aaggtccgca ccctgcaggt
      961 ctttctggta cgcatattca ctttattgac ccggtaggtg caactaaaca agtttggtat
     1021 ttaaattacc aagatgtaat tgctatcggt aaattattta cgaccggtga gctatttact
     1081 gaccgtgtag tcgcattagc cgggccacaa gtgaaaaatc cacgtttggt gcgtactcgt
     1141 ttaggggcga atctttcaca attaacagcc aacgaattaa atgcgggtga aaaccgtgtc
     1201 atttcaggtt ctgtgttaag tggtgctatc gcaacgggag tacacgacta tttaggtcgt
     1261 tacgcattac aagtgtctgc tttattagaa gggcgtgaga aagcgttctt cggtatgatt
     1321 gctccttact ctgataagta ttcaattacg cgtactactt tagggcattt tgcgaagaaa
     1381 ttatttaact tcacaacagc agttcacggt agtgagcgtg caatggtacc aacaggggct
     1441 tatgagcgtg taatgccgtt ggatatcatt ccaacactct tattgcgtga tttagcagct
     1501 ggagatacag actccgctca agctttaggc tgcttagagt tagacgaaga agatttagcg
     1561 ttatgtactt ttgtgtgttc cggtaaaaac aactggggac cattattacg tcaagcgtta
     1621 gataagatcg agaaggacgg ttaaaaatgg gtttgaaaaa tctttttgaa aaaatggaac
     1681 ccgctttcca taaaggtgga aagtatgaga aatggtacac gctatttgaa gctgcctata
     1741 cgattttcta tacaccgggt acggtcactc gtaaagatgc tcacgtgcgt gatgcgattg
     1801 actctaagcg tatgatgtta attgtatggt tagcgttatt ccctgcgatg ttctacggaa
     1861 tgtacaatgt tggtgcacag ggtattctag cggcactcaa aatgggaaca ttaacggatt
     1921 tagttgctaa caactggcac tacagcttag ccgatagttt aggctctgta gctgatgcag
     1981 gctggggaac taaaatgttg ctgggggcaa cctatttcct accaatctac ttaacagtat
     2041 ttgcagtggg cggtttctgg gaagtggtgt ttgcaatggt gcgtaagcac gagatcaacg
     2101 aaggtttctt tgtaacctct atcttactag cattaattgt tcctccaaca ttaccgttat
     2161 ggcaagcagc ccttgcaact acctttggtg ttgtggtcgc aaaagaagtg ttcggtggcg
     2221 tgggtaaaaa ctttatgaac cctgcgttag ccggtcgtgc tttcttgttc tttgcttacc
     2281 cgggtcaaat ttcaggtgat gcagtatggg ttgccgcaga cggcttctca ggtgcaactg
     2341 cactttctca atgggcagtt ggtggtcaag gtgcattaca gcacgttgca accggtgaaa
     2401 ccattacttg gatggatgcg ttcttaggta atattccggg ttctatcggc gagacctcta
     2461 ccttaatgtt gattattggt gctgcaatca ttgtatttgc ccgaattgct tcttggcgaa
     2521 ttattgccgg tgtaatgatt ggtatggcgg caactgcgac attatttaat gtaatcggtt
     2581 ctgataccaa cccattattc tctatgcctt ggcattggca tttagtatta ggcggttttg
     2641 cgatcggtat gttctttatg gcaaccgacc cggtatcagc tgcatttacc aacaaaggta
     2701 aatgggcata cggtatctta atcggcttta tgtgtgtatt aatccgtgtg gtaaacccag
     2761 cataccctga aggtatgatg ttagcgatct tatttgctaa cttatttgct ccaatctttg
     2821 actatttagt agttcaaggg aatatcaaac gcagattagc gagggtagca aacaatggct
     2881 aaatttaata aagatagcgt tggcggaacg ctaaccgttg tagttttatt aagtttaatt
     2941 tgttctttga ttgtggcagg tgcggcagta atgcttaaac caacacaaga tattcagaaa
     3001 caacttgata aacagaaaaa catcttacaa gcggccggtt tattgcaaaa agataccaat
     3061 gtgcaagaaa cctataccaa atttattgag ccgaaaattg tcgatttagc aacaggtgat
     3121 tacgttgagg gtgtaaccgg ttttgatgct cgtgcagcag ctaaagatcc tgctcaaaat
     3181 gtagttatcg cacctgaaga tgataaggcg aatatcaaaa cgcgtgctaa atatgctgaa
     3241 gtttatttag taaaagatga atctggcaaa acgactcaag tagtattacc aatgcacggt
     3301 aacggcttat ggtcaattat gtacggtttc gttgcggtac aacctgatgc aaataccgtt
     3361 aacggtatta cttactatga acaaggtgaa acggcaggct taggtggcga aattgcaaac
     3421 ccgaactggc agaaaaactt tgtaggtaaa aaactgtata acgagcaaaa ccaagttgcg
     3481 ttaagagtgg gtaaaggggc atctgcagat aaagatcacg gtgttgatgg tttatctggt
     3541 gcaacattaa cctcgaacgg tgttgatggt tcattcaaat actggtttgg tcaaaatggt
     3601 tttggtccat atttagcaaa atttaaagca gcggcaggag ctaactaatg gcagacaata
     3661 acaatcttaa aaaattattg ttatcgccaa ttgtcgataa caacccgatc gcattacaga
     3721 tcttaggtat ttgttctgcg ttagcggtaa cgactcaatt acaaacagca gtagtaatgg
     3781 cgattgcggt aagtttagtt actgcattct caagtatgtt catttcaatg attcgtaact
     3841 atattccaaa tagtatccgt atcattgtgc aaatggcgat tattgcatca ttagtaattt
     3901 tggttgacca aatcttacgc gcttatgctt acgatctgtc aaaacaatta tcggtattcg
     3961 taggccttat tattaccaac tgtatcgtaa tggggcgtgc agaagcattc gcgatgaaat
     4021 caggcccggt tgaaagtttt gttgatggta tcggtaacgg tttaggctac ggtgcaatgt
     4081 taattatcgt tgccttttta cgtgaactta tcggttcagg taaaattttc ggtatcacca
     4141 ttcttgaaac tgttcaaaat ggcggttggt atcaagcaaa cggtttattc ttattagcac
     4201 caagtgcgtt ctttattatc ggatttgtga tctgggcgat tagaacgtgg aaaccggaac
     4261 aagtggagaa ataagatgga acatcttctg agtatctttg ttaagtccat atttattgaa
     4321 aatatggcac tttctttctt ccttggtatg tgtacattcc ttgcggtgtc taagaaagtt
     4381 tcaactgctt ttggtttagg tattgcggta attgtagtac ttggcattgc agttccagct
     4441 aaccaattag tttataccca cgtattaaaa gagggtgcat tagtagaagg tgtggattta
     4501 agcttcttaa actttatcac tttcatcggg gttattgcgg cacttgttca aattctggaa
     4561 atgattttag acaagttttt cccggcactt tatagtgcat taggtatctt cttaccactt
     4621 atcaccgtaa actgtgcgat ttttggtggt gtatcgttta tggtacaacg tgagtataac
     4681 tttgtagaat ctgtggttta tggcgtaggt gcaggtacag gctggatgtt agccatcgtt
     4741 gcccttgcag gtttaaccga gaaaatgaaa tattccgacg tgccggcagg cttacgtggt
     4801 ttaggtatta cctttattac ggtaggctta atggcgttag gctttatgtc attctcaggc
     4861 attcaattat aaggagcata tcgtggatag taattttatt ttcggtatta tcgcatttac
     4921 ggctctggta ttagtgcttg cggtgatcat tcttttcgct aaatcaaaat tagttgattc
     4981 aggtgatatt accatctcaa tcaacaacga tccggaaaaa ggcattacgc taccggctgg
     5041 tggaaaatta ctcggtgctt tagcaagtaa agggattttc gtttcgtcag cttgtggggg
     5101 cggtggctct tgcggtcaat gtaaagtaca agtgaaatca ggcggtggtg aaattcttcc
     5161 aactgagtta tcgcacattt ctaagaaaga agcgaaagaa ggctggcgtt tagcttgtca
     5221 ggtaaacgtg aaatcttcaa tggacgttga acttccggaa gagatctttg gtgtgaaaaa
     5281 atgggaatgt accgttatct caaacgataa caaagcaacc ttcatcaaag agcttaaact
     5341 tcaaattcct gaaggcgaag aagtaccgtt ccgtgcgggt ggttatatcc aaattgaagc
     5401 tgaacctcat accgttcatt ataaagactt cgatattcca aaagagtacc acgaagactg
     5461 ggataaattt gacttatggc gttatacttc aaaagtggac gagcatatta ttcgtgcata
     5521 ctcaatggct tcatatccgg aagagaaagg cattattatg cttaacgtgc gtattgctac
     5581 acctccacca cgcaatccgg acgtaccgcc gggtcaaatg tcttcctaca tttggtcatt
     5641 aaaaccgggt gataaagtaa caatttcagg tccattcggt gagttcttcg ctaaagatac
     5701 cgatgcggaa atggtcttta tcggtggtgg tgcaggtatg gcaccaatgc gttcacatat
     5761 cttcgaccaa ttaaaacgtt taaaatctaa acgtaaaatg tcattctggt atggtgctcg
     5821 ttctgagcgt gaaatgttct atgtggaaga ttttgatggt cttcaagcag aaaacgataa
     5881 cttcaaatgg tatgtggcac tttctgatcc attaccgggc gacagagaag attacttccg
     5941 tggctttatt cataacgtgc tttatgagaa ctacctcaaa aaccacgaag cacctgaaga
     6001 ctgcgaatac tatatgtgtg gtccaccggt gatgaatgct gcggtaatcg gtatgctgaa
     6061 gagtttaggt gtagaagacg aaaacatctt attagatgac tttggtggtt gataaacctt
     6121 aacttctgac tacaagcg
//
LOCUS       DANLXS010000144         6110 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.145, whole genome shotgun sequence.
ACCESSION   DANLXS010000144 DANLXS010000000
VERSION     DANLXS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.145"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            93..1082
                     /gene="dusB"
                     /locus_tag="PXD71_002059"
     CDS             93..1082
                     /gene="dusB"
                     /locus_tag="PXD71_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL6292138.1"
                     /translation="MQIGQYAIKNRIFLAPMAGITDQPFRRLCSQLGAGLTFSEMMST
                     NPDVWHTEKSRLRLAHHDEIGLNAVQIAGSDPVEMAKAAKINVEYGADIIDINMGCPA
                     KKVNKNMAGSALLRNPKLVAQILDSVVNAVDVPVTLKIRTGWDQENRNCLQIAKIAEQ
                     AGIKALTIHGRTRSCLFNGEAEYESIKAVKEAISIPVIANGDIDSAEKAKFVLDYTKA
                     DAIMIGRASFGNPWIFKEINDFFETGLSSPLSIDKKYQLMFKHIEDLHQFYGEEKGYR
                     IARKHVGWYTEKLNPESNFKRTFNTLSSTKEQLYALEDFVKSIHLESQLRKVG"
     gene            1087..1383
                     /gene="fis"
                     /locus_tag="PXD71_002060"
     CDS             1087..1383
                     /gene="fis"
                     /locus_tag="PXD71_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDL6292139.1"
                     /translation="MLEQQPTQNPLTVTMLNAQAQQVNKPLRDNVKAALKNYLSQLNG
                     EDPTELYELVLSEIEHPMLDMVMQYTRGNQTRAATMLGINRGTLRKKLKKYGMG"
     gene            complement(1488..4274)
                     /locus_tag="PXD71_002061"
     CDS             complement(1488..4274)
                     /locus_tag="PXD71_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HDL6292140.1"
                     /translation="MKYHHFRYSPVALTVLFALSHSYGAATENKKIEENNDLAVLDEV
                     IVTESHYAHERQNEVTGLGKVVKNYHEMSKNQILGIRDLTRYDPGISVVEQGRGASSG
                     YAIRGVDKNRVSLLVDGLPQAQHYHTLGSDANGGAINEIEYENIRSIELSKGASSAEY
                     GSGALGGAIGFRTKDAQDIIKEGQHWGLDSKTSYASKNSHFLQSIAAAGEAGGFEALV
                     IATHRHGKETKIHSEANKLKHNIRRITGFENRYDLYPNSAQNAPGGSFFIVEDTCPTL
                     DCTPRARVKLNRDNFPVRTFPEYTPEERKQLEQIPYRTEQLSAQEYTGKDRIAPNPLD
                     YKSNSVFMKFGYHFNSSHYLGAILEDTKQRYDIRDMQTPAYYTKDDINLSLRNYVYEG
                     DNILDGLVFKPRIPYGLRYSHVKFFDERHHKRRLGFTYKYKPENNRWLDSIKLSADKQ
                     DIELYSRLHRLHCSDYPVVDKNCRPTLDKSWSMYRTERNNYQEKHRVIHLEFDKALNA
                     GQGVFKQTHKLNLGLGFDRFNSLMDHGDMTAQYTKGGYTSYRGRGRLDNPYIYRRDPR
                     SIETVSLCNNTRGDILNCEPRKIKGDSHFVSFRDLVISEYVDLGLGVRFDQHRFKSDD
                     PWTLSRTYRNWSWNGGITLKPTEFVSLSYRISNGFRVPAFYELYGKRDHIGLKDNEYV
                     QRAQRSHQLEPEKSTNHEIGVSFKGQFGYLDVSYFRNNYKNMIATACKRIIQKSHCFY
                     NYHNIQDVALNGINLVAKFDLHGILSMLPDGFYSSVAYNRVKVKERKLTDSRLDSVND
                     PILDAIQPARYVLGFGYDHPEEKWGIGITTTYSKAKNADEVAGTRHHGIHRVDLGGKL
                     TGSWYTHDITGYINYKNYTLRGGIYNVTNRKYSTWESVRQSGVNAVNQDRGSNYTRFA
                     APGRNFSLAFEMKF"
     gene            complement(4368..>6110)
                     /locus_tag="PXD71_002062"
     CDS             complement(4368..>6110)
                     /locus_tag="PXD71_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL6292141.1"
                     /translation="SSFVLLNAALLAACSSNGGSFDVQSAKVESQTQTTPKKPSLQDD
                     NSNARRTVSASETEALLQPGFGFSAKIPRRNLFPQGKEDVAPIGDIKEITGDLTKIPY
                     EEEVKAYGSSADGFSHTHDRNHKLYTRDFNFVRSGYVLHSGAKPEIKPKEILRTGAHG
                     YVYYLGIEPAKAIPTQKATYKGYWDFTTDARKGRDSKYFSNSAGINIGATPENSYDIN
                     VDDSEKPMGHTGEFTADFANKTLTGTLVRNGYVSRSKEQKITTIYDIDAKIKGNRFSG
                     KANAKNTDDPYFGKSSTTLEGGFFGGKAQELAGKFLADDKSVFVVFAGKRDAKKDDSE
                     SAFDAFKIKLKDLNKSEMDTFGNATHLIINNKQIPLIAEGKKSFAEMKFYDLVTRTID
                     DKTYRVSVCCNNLDYVKFGTYSEGNNSDTAHQYLVGERTAVADLPTGTVKYRGTWDGV
                     MYSKSGSAGAESPSNSESGTRSLFDVDFVNKKINGKLIANDGVEERPMLTLEGNLKGN
                     GFAGTAKTGNSGFNLDPKSTNGGTVVHINTQFEGGFYGPKATELGGIVHSAETDKDRV
                     SITFGGKRQIQIEK"
BASE COUNT         1855 a         1273 c         1084 g         1898 t
ORIGIN      
        1 gctattataa gaaagtacgg tatttaccgt actttttatt tggatatccc tctggttaaa
       61 attacttcaa atttgttaat aaaaggttaa aaatgcaaat aggacaatat gcaatcaaaa
      121 atcgtatttt ccttgcacct atggctggaa tcacagatca gccttttcgc cgcttatgta
      181 gccaacttgg agcaggttta acattttcag agatgatgtc taccaatcct gatgtatggc
      241 atactgagaa atcacgattg cggttagcac accacgatga aataggctta aatgccgtac
      301 aaattgcagg ttctgatcct gttgaaatgg caaaggctgc aaagataaat gttgagtatg
      361 gtgccgatat tattgatatt aatatgggtt gcccggcaaa aaaggtgaat aaaaatatgg
      421 caggctctgc tcttttacgt aaccccaaat tagtcgctca aatattagat tctgtagtca
      481 atgccgttga tgtccctgtg acactaaaaa ttagaacagg ttgggatcag gaaaatagaa
      541 attgtctgca aattgcgaaa attgcagaac aagccggcat aaaagctctc actattcacg
      601 ggcgtactcg aagctgttta tttaatggcg aagctgaata tgaaagtatt aaggcagtaa
      661 aagaagcaat ttctatccca gtgatagcaa acggagacat tgactctgcg gaaaaagcaa
      721 agtttgttct cgactatacc aaagccgatg caatcatgat tgggcgcgct agctttggaa
      781 atccttggat atttaaggaa attaatgatt tttttgaaac tggattatct tcaccattat
      841 caattgataa aaaataccaa ttaatgttta aacatattga ggatttgcac caattttatg
      901 gtgaagaaaa aggttatcgt attgcacgaa aacatgttgg ttggtatacc gaaaaattaa
      961 atccggaatc aaattttaaa cgcactttta atacactaag ttccacaaaa gagcaacttt
     1021 atgcattaga agattttgtt aaatcgattc atttagaatc tcaattaaga aaagttggat
     1081 aaaaaaatgt tagagcaaca acctacacaa aacccattaa cagtaacaat gttaaatgca
     1141 caagcgcagc aggttaataa accacttcgc gataatgtga aagcagcatt aaaaaactat
     1201 ttatctcaat taaatggtga agatccaaca gaattatacg aattagtatt atctgaaatc
     1261 gaacacccaa tgttagatat ggtgatgcaa tatactcgcg gcaatcaaac tcgtgcagca
     1321 acaatgctag gcattaaccg tggcacatta cgcaaaaaat taaaaaaata cggtatgggt
     1381 taatctaacc gaccgttaaa acacgatttt aattcgtatg taattagtta gagccaacaa
     1441 aaaacgctga atatactatt cagcgttttt ctattttatt ttgccgtcta aaacttcatt
     1501 tcaaatgcta aactgaaatt tctccccgga gcggcaaatc gagtgtaatt gctaccccgg
     1561 tcttggttta ctgcattcac accggattgg cgcactgatt cccaagtgga atatttacga
     1621 ttagtcacat tataaattcc tccacgtaag gtgtagtttt tataattgat gtaaccggta
     1681 atatcatggg tgtaccaaga accggtcagt ttgccaccta aatcaacgcg atgtatgccg
     1741 tgatgacgtg tgcctgccac ctcatcggcg tttttggctt tagaataggt ggtagtaatg
     1801 ccaattcccc atttttcttc tgggtgatcg tagccgaatc caagcacata gcgtgctggc
     1861 tgaatcgcat ctagaatagg atcgtttacg ctatcgagtc ttgagtcggt tagtttccgc
     1921 tcttttactt ttacacggtt ataagcaact gatgaataaa aaccatctgg cagcatagat
     1981 aaaataccgt gtaagtcaaa tttagcgact aaatttatcc cgtttagtgc tacatcttga
     2041 atattatggt agttatagaa acagtgtgat ttttgtatta ttcttttaca tgctgtcgca
     2101 atcatatttt tatagttatt acggaaatag ctcacatcaa ggtaaccaaa ttgaccttta
     2161 aagctaactc caatctcatg attagtcgat ttttctggct ctaactggtg gctacgttgc
     2221 gcgcgttgca catattcgtt atctttaagc ccaatatgat cacgtttacc ataaagttca
     2281 tagaatgcag gcactctaaa accgtttgaa atgcgataag aaagcgatac aaactctgtt
     2341 ggtttaagcg taatcccacc attccaagac caatttcgat aagttcggct aagtgtccac
     2401 ggatcatcag atttaaatcg atgttgatca aaacgcaccc ctaatcccaa atccacatac
     2461 tcgcttatca ctagatcgcg gaagctaaca aaatggctat cgcctttaat tttacgcggt
     2521 tcacagttta agatgtcgcc gcgtgtatta ttacacaaag ataccgtttc aatactgcgt
     2581 ggatcgcggc gataaatata tggattatct aaacgccctc taccgcggta gctggtataa
     2641 ccgcctttgg tatattgggc agtcatatcc ccatgatcca taagcgaatt aaatcgatca
     2701 aagcccaacc ctaaattcag tttgtgggtt tgcttaaata cgccttgacc agcatttagc
     2761 gctttatcaa attctaaatg aatgacacga tgcttttctt ggtaattatt acgctcagtt
     2821 cgatacatag accaagattt atccaaagtc gggcggcaat ttttatctac cacaggataa
     2881 tcgctacaat gcaagcgatg tagccggcta tatagttcaa tatcttgttt atccgcactg
     2941 agtttaatgc tatccaacca gcgattattc tctggtttat atttataggt gaatcctaaa
     3001 cgacgtttgt ggtgacgttc atcaaaaaac ttcacatggc tatagcgcaa cccataaggg
     3061 atccttggct tgaacactaa gccatctaaa atattatccc cttcataaac atagttccta
     3121 agtgataagt taatatcgtc ttttgtatag taagctggcg tttgcatatc acggatatcg
     3181 tagcgttgtt ttgtatcttc taagattgcg ccaagataat gagacgagtt aaagtgatag
     3241 ccaaacttca taaaaacaga attactcttg taatctaaag ggtttggtgc aatgcgatct
     3301 ttaccggtat attcttgggc tgagagctgc tcagtgcgat aaggaatctg ctcaagctgt
     3361 ttgcgctctt caggcgtata ttccggaaat gttctcactg ggaaattatc gcggttcaac
     3421 ttaacccttg cacgaggagt acaatctaat gttgggcaag tatcttccac aataaaaaaa
     3481 gatcctccag gagcattctg tgcggaattt gggtaaagat cgtagcgatt ttcaaagccg
     3541 gttatacgcc gaatattatg ttttaattta tttgcctcgg aatgaatttt ggtctcttta
     3601 ccgtgtcggt gagttgcaat aacaagtgct tcaaaaccac ccgcctcacc agccgctgcg
     3661 atagactgta aaaaatggct atttttgctg gcataagagg tcttactatc taagccccaa
     3721 tgctgcccct ctttaataat atcctgcgca tctttagtac gaaaaccaat agcaccacct
     3781 agcgcaccag agccatattc cgcagaactt gctcctttgc ttaactcaat tgaacgaatg
     3841 ttttcatact caatctcatt aattgcacca ccattagcat ctgaacctag cgtatgataa
     3901 tgctgcgctt gtggtagccc atcaacaagt aagctgacac ggtttttatc tacacctcga
     3961 atggcatagc cactacttgc accgcgacct tgttccacca ccgaaatacc agggtcatag
     4021 cgagttaaat cacgaatacc aagaatttga tttttactca tttcgtgata atttttcact
     4081 actttcccca agccagttac ttcgttttga cgttcgtgag cataatggct ctctgtcaca
     4141 ataacttcat ccagaacagc tagatcgtta ttttcttcta tttttttatt ttcagtcgca
     4201 gcaccgtatg aatgagaaag agcaaataac actgttaagg caacaggtga atagcgaaaa
     4261 tgatgatatt tcattatcat tagatagggt ctcctttatt taaattttgc aaatttttat
     4321 aaaaaattgc ccgcttgcta tctacaacca gcaaagggga attatgatta tttttctatt
     4381 tgtatttgac gttttccgcc gaatgtaata ctgactctat ctttatccgt ttctgcacta
     4441 tgtacaatac cacctaattc cgtcgcctta gggccataaa agcccccttc aaattgagta
     4501 tttatatgca ctaccgtgcc accattcgta cttttcggat caagattaaa accagaattg
     4561 cccgttttgg ctgtgcctgc aaaaccattc cctttcagat tgccttcaag tgtcagcatt
     4621 gggcgttctt caacaccatc attagcaatc agcttgccat taattttttt attgacaaaa
     4681 tctacatcga atagtgaacg agtaccactt tcgctgttac ttggcgattc agcccctgcc
     4741 gagccagatt tactgtacat taccccgtcc caagtacctc gatattttac tgtccctgtt
     4801 ggcaaatctg ccacagctgt acgttctcct actaaatatt ggtgagcagt atcactatta
     4861 tttccctcgc tataagtccc aaatttgaca taatctaaat tattacagca gactgaaact
     4921 cgatacgttt tatcatcaat agtacgggta accaaatcat aaaatttcat ctcggcaaag
     4981 ctttttttgc cttccgcaat aagtggaatc tgcttattgt taataatcaa atgtgtcgca
     5041 ttcccgaaag tatccatctc agatttattt aaatctttaa gtttaatttt gaaagcatca
     5101 aaggcagatt cactatcatc ttttttagca tctcgtttgc cagcaaaaac aacaaatacc
     5161 gacttatcat cagctaagaa tttaccggca agttcttgag ccttcccacc aaaaaatcca
     5221 ccttcaagtg tcgtagagct tttaccaaaa taaggatcat cggtattttt tgcgtttgct
     5281 ttaccagaaa agcgattacc tttaattttt gcatcaatat cgtaaattgt tgtaattttt
     5341 tgctctttgc tacgactaac atacccatta cgaaccaatg ttccagttaa agttttatta
     5401 gcaaaatcag ccgtaaattc tcctgtatgc cccattggtt tttcagaatc atcaacatta
     5461 atatcgtaac tattttccgg tgtggcgcca atattgatgc ctgcggaatt agaaaaatat
     5521 ttactatctc tcccttttct cgcatcggta gtaaaatccc aatatccttt ataagtagct
     5581 ttttgagtag gtattgcttt tgccggctct atacctaaat agtaaacata cccatgtgca
     5641 cctgttctca aaatttcttt aggctttatt tcaggttttg caccagaatg cagaacatag
     5701 ccagaacgaa caaaattaaa atctctagta tacaacttat gatttctatc atgagtatgg
     5761 ctaaatccat cagcactact accgtacgct ttaacctctt cttcatacgg aatttttgtc
     5821 agatctccag taatctcttt tatatcacca ataggggcta catcttcctt cccctgcgga
     5881 aagagattac gacgcggaat tttggctgaa aaaccaaacc ccggctgcaa taaagcttca
     5941 gtttcagaag cgcttactgt acgtcttgcg ttactattat catcttgtaa acttggcttt
     6001 ttgggggtag tttgcgtttg agattcaact ttggcagatt gaacatcaaa gcttccacca
     6061 tttgaggaac aagcagcaag tagcgccgca ttaagcagta caaaactact
//
LOCUS       DANLXS010000145         6069 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.146, whole genome shotgun sequence.
ACCESSION   DANLXS010000145 DANLXS010000000
VERSION     DANLXS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.146"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            82..1899
                     /locus_tag="PXD71_002063"
     CDS             82..1899
                     /locus_tag="PXD71_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDL6292142.1"
                     /translation="MIIKDTHWTSQQASPNAFPERQALMPIWGGKNIERNQWQSLWLE
                     QAPNALLQDGLAYFHIPFCASHCIFCGFYRNAWKENYSQIYTDRVISELRYESSLWRG
                     QGKIHAVYFGGGTPTALHTEDLVRLIRACYRYLPLAEDCEFTIEGRISHFDIDKAKAC
                     IEAGVNRISIGIQTFNSEIRKRLGRKHSGEEAQTYLKALSELNAVIVADLIFGLPNQT
                     DDIWQNDIEIATSLPISGLDIYAFNNYPFLPINRLIEHGKLPAPAGFDTQSLHYAYAV
                     EKLKAQGWQQVSNNHFAFPNRGERNRYNTLVKSNMPCLAFGSGAGGNFGGLSYQVQSD
                     LKTYLDSPLDQKSLSFLSKHGEHKALLGVIQHSMELGYLDTTPFEQNAKAEKLLQQWE
                     ALDLLKREKNQVYLNTSGRYWSPTLIRKLMLTLPQSSKDNNMITPLTKELENKLRTNL
                     KENPGQILEMLAGMNQCSLESVINCLPKEMVMQTGGERFVEIMQAIAGWNEAVTFIIH
                     TPDAIAEVTAKLPQGKIGRGFYNFEHGVDGGIHGHLRYENCAAIYLLDRPFMGKRTVA
                     LTFINQSGEAMFKIFVGRDEQGQLRENQIQAMRKLIGGC"
     gene            1902..2519
                     /locus_tag="PXD71_002064"
     CDS             1902..2519
                     /locus_tag="PXD71_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6292143.1"
                     /translation="MILVCGANGLLGRAICDKLPREKTIAVLRKDAMDTFFSERNIAV
                     EIADLLDLESCNALLQKTQPEILITVVGGKNEQGIRSDKTANINLIKAVEKYSPTTKV
                     VLVTSVGCAEQWDLISPLAQQALGEALKAKTEAEAYLQQTALNWLIIRPGGLNNDEVN
                     DEYQIVRDLPNERKMYVSRKSVATGIVRLLDSTENHQIFSIINRG"
     gene            complement(2603..3214)
                     /locus_tag="PXD71_002065"
     CDS             complement(2603..3214)
                     /locus_tag="PXD71_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentahemic C cytochrome"
                     /protein_id="HDL6292144.1"
                     /translation="MKSLSKIAFCFLAFIMPTFCFAGSNSYTTETIPLFADENLTQKI
                     GQLEAGAEIKLVQTTAKAAEIELNLWRKSKGFGRIWYHSFSKNITDAVFEKSFISNNP
                     LLEIVESKEDPNTGLVWQKVKMNVWVENAEFTQDLTNFWANTKNTYKTECSVCHKQRD
                     PKMHDANEWIAVFNGMVGFTDMDEATQKKVLRYLQMNAASSSQ"
     gene            3409..4317
                     /gene="glyQ"
                     /locus_tag="PXD71_002066"
     CDS             3409..4317
                     /gene="glyQ"
                     /locus_tag="PXD71_002066"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL6292145.1"
                     /translation="MSTKFNVKTFQGMILALQDYWASKGCTIVQPFDLEVGAGTSHPM
                     TCLRALGPEPMAFAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLDS
                     LKMLGFDPTQNDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPV
                     TGEITYGLERLAMYIQGVDSVYDLVWSDGELGKTTYGDVFHQNEVEQSTYNFEYADVD
                     FLFQCFEQYEKEAQYLLGLEKPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILR
                     IRALTKGVAEAYYASREALGFPGCKK"
     gene            4397..4657
                     /locus_tag="PXD71_002067"
     CDS             4397..4657
                     /locus_tag="PXD71_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292146.1"
                     /translation="MKPILYFADWCPDTAPFVAELDRLGIEYEPCDITKGGSTLKPFL
                     KLRDTHSAFDHAKANGYIGIPALLIEGEKVVLDLAELEGIFG"
     gene            4700..5734
                     /locus_tag="PXD71_002068"
     CDS             4700..5734
                     /locus_tag="PXD71_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL6292147.1"
                     /translation="MKTVIADNSAEFVNEISQLIQSSKQRMTVAVNAELTLLYWHIGK
                     RINDYILQGERAEYGQEVVKNLAQSLTEQFGKGWSKRHLNYTMQFATTFPNLEIVHAL
                     RAQLSWTHFKQIIHIDDPTKRGFYATMAAQERWSTRTLEDRIGSLLFERTAISKKPDE
                     TIIAELSLLREKGEYNQSMVLKDPYILDFLDLNDRYMERDLEDAILREIEQFLLELGA
                     GFTFVARQKRIQIDNDDFYIDLLFYNRKLKRLIAIELKTESFKHSHKSQMELYLAWLA
                     KYEQEADENPPLGMILCTGKKQEQVELLDLKNSDIHIAEYMTVLPSKAVLEQRLHLAV
                     ERAKERFLLK"
BASE COUNT         1865 a         1134 c         1338 g         1732 t
ORIGIN      
        1 ttatttgatt ttaatcaagt cattcataac ttaattttgt tataaatatg accttgttta
       61 tagcactatt ttgtttcgat aatgattata aaagatacac actggacatc tcaacaagcc
      121 tctccaaatg ctttcccaga acgacaggca cttatgccta tttggggtgg gaaaaatatt
      181 gaacgcaatc aatggcaatc tttatggctg gaacaagcac caaatgcgtt attacaggat
      241 ggtttagctt atttccatat tccgttttgc gctagtcatt gtattttttg tggtttttat
      301 cgcaatgctt ggaaggaaaa ttatagccag atttatactg atcgtgtgat tagtgaactt
      361 cgatatgaat cttcactatg gcgaggacaa ggtaagattc atgccgttta ttttggtgga
      421 ggaacgccaa cagcgttaca tacagaagat ttagtacgat tgattcgagc ttgttatcgt
      481 tatttgccac tcgctgaaga ctgtgaattt actattgagg gtagaattag ccattttgat
      541 atagataagg ctaaggcttg tattgaagca ggcgtgaacc gtatttctat tggtattcag
      601 acttttaact ccgaaattcg caaacgttta gggcgcaaac atagtggcga agaggctcaa
      661 acttatttaa aagccttatc ggaattaaat gctgtgattg ttgctgattt aatttttggc
      721 ttgccaaacc aaacagatga tatctggcaa aacgatattg aaattgcgac tagtttaccg
      781 atctcaggct tagatattta cgcctttaat aattatcctt tcctgcctat taatcgctta
      841 attgaacatg gaaaattacc tgcgcccgca gggtttgaca cccaaagctt acactatgct
      901 tatgcagtag agaagttaaa agcacaaggt tggcagcaag tgagcaataa ccactttgct
      961 tttccaaatc gaggtgaacg caatcgctat aatactttgg ttaaatcgaa tatgccttgt
     1021 ttggcatttg gctccggtgc ggggggaaat tttggtgggc tgagttacca agtacaatct
     1081 gatttaaaga cctacttaga ttcgcctctt gatcagaaaa gcctttcttt tttgagtaaa
     1141 catggtgaac ataaagcact gcttggtgtg atacaacata gcatggaatt gggttactta
     1201 gatacaacgc cttttgaaca aaatgcgaaa gctgaaaaat tattgcaaca atgggaagcg
     1261 cttgatttac tgaagcgtga aaaaaatcaa gtatatctca ataccagtgg tcgttactgg
     1321 tcaccaacat taattcgtaa actaatgtta acacttcctc aatcaagtaa ggataacaac
     1381 atgataacgc cattaactaa agaattagaa aataaattgc ggacaaattt aaaagaaaac
     1441 ccagggcaaa ttttagaaat gctggcagga atgaatcaat gtagcttgga atcggtaatt
     1501 aactgtctgc ccaaagaaat ggtaatgcaa acaggtggtg agcgttttgt ggaaattatg
     1561 caggcaattg ctggttggaa tgaagctgtg acttttatta ttcatacgcc tgatgcgatt
     1621 gcagaggtaa ctgctaaatt gccacaaggt aaaattgggc gaggttttta taattttgaa
     1681 catggtgtag atggaggaat tcacggtcat ttacgctatg aaaattgtgc tgctatttac
     1741 ttacttgatc gcccatttat gggaaaaaga acggtggcgt taacttttat caatcaaagc
     1801 ggtgaggcga tgtttaagat ttttgttggg cgtgatgaac aaggtcaatt acgagaaaat
     1861 cagattcaag ccatgcggaa attaattgga ggatgctagt catgattttg gtttgtgggg
     1921 caaatgggtt gttaggacga gcaatctgtg acaaattacc tagagagaaa acgatagccg
     1981 tcttacgaaa agatgctatg gatacgtttt tcagcgaacg aaatatagca gtagaaatag
     2041 cagatttatt agatttggaa tcctgcaacg ccttgttaca aaaaacacaa ccggaaatct
     2101 tgattacggt agtaggagga aaaaatgaac aaggtattcg tagcgacaaa acagcaaata
     2161 ttaatttgat aaaagcagtt gaaaaatatt caccaactac gaaagtagtt ttagttacta
     2221 gtgttgggtg tgcagaacaa tgggatttaa ttagcccatt agcacagcaa gctttaggtg
     2281 aagcactaaa agccaagacg gaagctgaag cttatttaca gcaaacagca ttaaattggc
     2341 tgattattcg acccggtggg ttaaataatg atgaagtaaa tgatgagtat cagattgtgc
     2401 gtgatttacc taatgaacgg aaaatgtatg taagccgtaa atctgtcgca acaggcatag
     2461 tgcgtttact ggatagtacg gaaaatcatc aaatcttctc tattattaat aggggatagt
     2521 atatttcaac gaaggcaagc gggcatattt tatggttatt ttgcaactat aaaacagacc
     2581 gcttgtgaga tctttttcta ttttattgag aactggatgc cgcattcatc tgcaagtagc
     2641 gaagcacctt tttctgggta gcttcgtcca tatcggtaaa accgaccatt ccgttaaata
     2701 ctgcaatcca ttcgtttgca tcatgcattt taggatcacg ttgtttatgg cacacgctgc
     2761 attctgtttt ataggtattt ttggtgttcg cccaaaagtt agtgagatct tgggtaaatt
     2821 cggcattttc cacccacacg ttcattttca ctttttgcca taccaagccg gtgttcggat
     2881 cttccttgct ctctacaatc tctaacagtg ggttgttgct gataaaagat ttctcaaata
     2941 ccgcatcggt aatattttta ctgaaagaat gataccaaat ccgcccgaaa cctttgcttt
     3001 ttcgccataa attcagctca atttcagcgg cttttgcggt tgtttgcacc aatttaattt
     3061 ctgctccggc ttctagctgc ccgattttct gtgtcaaatt ttcatcggca aataaaggga
     3121 ttgtttccgt ggtgtaactg tttgaaccgg caaaacaaaa tgttggcatg atgaatgcca
     3181 agaaacagaa agcgattttc gataatgatt tcatcataaa ccccacaaat tagatgagaa
     3241 attaggttat atgttacaca aattgagcaa aaaagcgttg atttacatca taaaactgat
     3301 tttatgtcga tttcttaatt ttttgcaaaa aaatcccaaa aaatgactgc ttacaagcta
     3361 taataaccag tatttttcga ccatttttca aattcaagag aaaataaaat gagtacaaaa
     3421 ttcaacgtaa aaacctttca aggaatgatt ttagccctgc aagactactg ggcaagcaaa
     3481 ggttgcacca ttgtgcagcc gttcgattta gaagtggggg caggcacctc gcacccaatg
     3541 acctgcctgc gtgcgcttgg ccctgagcca atggcgtttg cctatgtgca accatctcgc
     3601 cgcccgacag acggtcgcta cggcgaaaat ccgaaccgtt tacagcacta ctaccagttc
     3661 caagtggtga ttaaaccttc gccggataac attcaagaac tttacctcga cagcctgaaa
     3721 atgctcggtt tcgacccaac ccaaaacgac attcgttttg tggaagacaa ctgggaaaac
     3781 ccaacgcttg gggcgtgggg cttaggttgg gaagtgtggc tgaacggaat ggaagtgacc
     3841 caattcacct acttccagca agtcggcggc ttggaatgta aaccggtgac aggcgaaatc
     3901 acctacggct tagagcgttt ggcgatgtac attcaaggcg tggacagcgt gtacgacctc
     3961 gtgtggtctg acggcgaact cggtaaaacc acctacggcg acgtattcca ccaaaacgaa
     4021 gtggagcaat ccacctacaa ctttgaatac gctgatgtgg acttcctgtt ccaatgcttc
     4081 gagcagtacg aaaaagaagc ccaatacctg ttaggcctag aaaaaccatt gccattgccg
     4141 gcatacgaac gcattttaaa agccgctcac agctttaact tattagatgc tcgtaaagcg
     4201 atctcggtaa ctgaacgcca acgctacatt ctacgcattc gtgccttaac caaaggcgtt
     4261 gctgaagcgt actatgccag ccgtgaagcg ttggggttcc cggggtgtaa aaaataatcg
     4321 tagggacgcc acgcttggcg tccgtcaata aattacaaaa cggacacgaa gcatcgtgtc
     4381 cctacaagga tttacgatga aaccgatttt atacttcgcc gattggtgtc cggataccgc
     4441 cccatttgtg gcagaactag accgcttggg cattgaatat gagccgtgcg acatcaccaa
     4501 aggaggctca acacttaaac catttttgaa actgcgagac acgcattctg cctttgacca
     4561 cgccaaagcc aacggctata ttggcattcc ggcattgctg atcgaaggcg aaaaagtcgt
     4621 gctggatttg gctgaattag agggtatttt cggctaacaa gcggttgaat ttggcggaaa
     4681 tgttgcaaag gagccaataa tgaagacagt aatagcagat aattcggctg aatttgtgaa
     4741 tgaaatttca caacttattc aatcgagtaa gcaacgaatg accgttgcgg ttaatgcaga
     4801 attaaccttg ctttattggc atatcggaaa acggatcaat gactatattt tgcagggtga
     4861 acgtgcggaa tatgggcaag aagtggtgaa aaatcttgct caaagtctga cggagcaatt
     4921 tggtaaaggt tggagtaaac gccatttaaa ttatacaatg caatttgcta caactttccc
     4981 taatttggaa attgtgcacg cactgcgtgc acaattaagc tggacacatt ttaagcagat
     5041 tattcatatt gatgatccaa caaagcgagg gttctatgca acaatggctg cacaggagcg
     5101 ttggagtaca aggacattag aagatcgcat tggctcatta ttgtttgaac gcacggcaat
     5161 ttccaaaaag cccgatgaaa ccattattgc tgaattgagt ttgctacgtg aaaaaggcga
     5221 atataatcag tcaatggtgt tgaaagatcc ctatattttg gattttctcg accttaacga
     5281 ccgttatatg gaacgtgatc tcgaagatgc gattctacga gagatcgaac agtttttact
     5341 ggaacttggg gcgggtttta cctttgtcgc ccgccaaaaa aggattcaga ttgataatga
     5401 tgatttctat atcgatctgc tgttttataa ccgtaaactc aaacgcctga ttgcgattga
     5461 actaaaaacc gaaagcttca aacattccca caaaagtcaa atggaactct atttggcgtg
     5521 gctggcgaaa tacgagcaag aagcagacga aaacccaccg cttggaatga ttctctgcac
     5581 cggtaaaaag caggagcaag tggaattgtt ggatttgaaa aattccgata ttcatattgc
     5641 ggaatatatg accgtgttgc cgagtaaagc ggtattggaa caacggttgc atttggcggt
     5701 ggaaagggcg aaagagcggt ttttactaaa atagcaagcg gttaaaatca gcggaaatgt
     5761 tgcaaaatga aagtatgatg gaaacgattt ggttgcatat tccgttggtg ggtggttaga
     5821 gattttcaaa tctcccccct ccccctcttt ggctaaagag ggggcgatca tttagtgagt
     5881 aattttaatt catctgacta tttacaatct taatgaagaa agatgaatta taaatttacg
     5941 aagatcaaat tccccttttt agcaaagagg ggctagggga gatttggcag aagtgaaaac
     6001 gtagtgagag attgattagt ctagtacttt tttgcaagtt aatcataaag ttgctctcat
     6061 ttcttgata
//
LOCUS       DANLXS010000146         5988 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.147, whole genome shotgun sequence.
ACCESSION   DANLXS010000146 DANLXS010000000
VERSION     DANLXS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.147"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(62..370)
                     /locus_tag="PXD71_002069"
     CDS             complement(62..370)
                     /locus_tag="PXD71_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL6292148.1"
                     /translation="MKKYIATMLLAFGLVHSSYAQEKTAQIETKQAEKTVVLHIPEMY
                     CQLCVYLVNKELRAVEGVISTKASMKERKATVVVKESVDNQALIQAVGNLKYTAKVIE
                     "
     gene            complement(372..740)
                     /locus_tag="PXD71_002070"
     CDS             complement(372..740)
                     /locus_tag="PXD71_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292149.1"
                     /translation="MNSSLKSYNHKFITMCVTAIVSAVTSTFCCIGPLVYLVFGISSP
                     WLMELNQLEFLQLPMLLISLAAFGYGFWLLNFSNKIICTKYFSRRTLVILYWVVSVVV
                     MFFLTYPYVLPYILEMWSGE"
     gene            complement(715..1821)
                     /locus_tag="PXD71_002071"
     CDS             complement(715..1821)
                     /locus_tag="PXD71_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HDL6292150.1"
                     /translation="MNKLIASLVASSCATAFAAPVQQNNFHTTTHTYEFTQSYDLVVP
                     QGSKGTTNLWIPLPSDTDYQTVKSIEFEGSYKNAYITENNAYGAKTLFATWDTDAPKR
                     DLKVKMVIQTQDREPMAQGALANYQMPEKITYSVDVLEYLKPTAHIKTDGIVKEYADK
                     IVGNEQNPLKKAELIHKWIVDNMERDNSVLGCGDGDVEKILTTKVLKGKCTDINSVFV
                     ALARASNIPAREVFGIRLGQAVKMGQYSKTAFGSSKDGVANESGGQHCRAEFYLAGFG
                     WVPVDSADVAKMRLAEKKDVDDADVQAVSKYLFGNWEMNWVGFNHGRDFDLYPQPELT
                     PLNNFGYPYAEVGGDPLNSFEAKEFGYEFISKEL"
     gene            complement(2061..3104)
                     /locus_tag="PXD71_002072"
     CDS             complement(2061..3104)
                     /locus_tag="PXD71_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL6292151.1"
                     /translation="MQPINIAIAGAGFSSRVFHIPFLKQDARFNIVKVYERTSNNAAE
                     WLPNAETVRDFNALLTPEVDLVLITTPNQTHYAMVKESLLAGKHVLVEKPLVATVAEA
                     EELAELAKRQGKVLYVYQNRRWDSHIATAKSLMEQNLLGELVDCEIRFDRYAKEKNVK
                     VWKESGELGTGLVYDLGVHLIDQALYLFGKPEAVYADVRYQHEGALVDDNFDIQLYYA
                     NGFKVVLKASRYAREPSPTFVLHGKLGSYLKQTPDNQEDLLKNGVQPIGKDWNIEEKE
                     NWGILHTEVEGNVVRQPYRNAEVSYQNLFDDLYQAIAHNAEPIIKLEDVIFVLKVIES
                     VFESAKLGQKIYL"
     gene            complement(3208..3492)
                     /locus_tag="PXD71_002073"
     CDS             complement(3208..3492)
                     /locus_tag="PXD71_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292152.1"
                     /translation="MRYWLISLSILLSGCVVPVTPTGQAGIPMPIIIGVSDPLDSKEV
                     HICTLKPFTQEYRSEHSSRGRAKLNVQKQCRANHSEMFCEEKDIVCKTYD"
     gene            complement(3522..3707)
                     /locus_tag="PXD71_002074"
     CDS             complement(3522..3707)
                     /locus_tag="PXD71_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL6292153.1"
                     /translation="MKCRQATELISQSCDEKLRLQQHMRLQLHLLVCPKCRNFNKNTK
                     KLGKILRAYCNDKDAPF"
     gene            complement(3704..4270)
                     /locus_tag="PXD71_002075"
     CDS             complement(3704..4270)
                     /locus_tag="PXD71_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL6292154.1"
                     /translation="MKSLSSTQIEEIRKQMVKFATLQLADKDLAEDVVQDALTNAYKH
                     SASFRREAALKTWIFAILKNKILDLLRTRKRHIPVSSLCEEEESPSAFFDRHDHWLEH
                     QDVTEWRGVAQSTYQKEFWAIFDICLNKLPAQQARVFMMREHLEMSSAEICQECEISL
                     SNLNVLLHRARLRLQECLSRNWFREESK"
     gene            4379..5332
                     /gene="msrP"
                     /locus_tag="PXD71_002076"
     CDS             4379..5332
                     /gene="msrP"
                     /locus_tag="PXD71_002076"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDL6292155.1"
                     /translation="MKKLTIQDVTPETIFYQRRKIITALGLGIAASALPKVSMAETQR
                     HLQSLQFKPDPNKGLELTPENKVIGYNNFYEFGVDKSAPAKNAANFKTDPWTLEIGGE
                     VENPFSLNHQQLLNTFPLEERIYRFRCVEAWSMVVPWIGFELARLIEMAKPTSKAKYV
                     VFHTLYDPAQMPGQKNRLLGGGIDYPYVEALRLDEALNSLTLLSVGLYGKTLPPQNGA
                     PIRLVVPWKYGFKSIKSIVKITFAEKRPLTTWESLAPNEYGFYANVNPNVDHPRWSQA
                     SERVIGAGGLLSVKRQPTLMFNGYENEVANLYRGLDLKVNY"
     gene            5332..5916
                     /locus_tag="PXD71_002077"
     CDS             5332..5916
                     /locus_tag="PXD71_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HDL6292156.1"
                     /translation="MLTWLRILIHLGCLLPLVWVAQLLYTGNETILGADPIKELEHFL
                     GYGAIVIFCIMFLLGIALQYLKKNQYQILRRPLGLWAFVWAALHISSYLLLELSLDVK
                     LFFSELAGRPYLILGAAAFFILSIMAITSLPMLKRKLGTRWGTIHQWAYPALVLAMVH
                     YYWSVKSVTFDPIIIGVLVLFIVAHKIQAKFAKK"
BASE COUNT         1782 a         1264 c         1141 g         1801 t
ORIGIN      
        1 atgggggaaa aaaggaaggc taaattaaaa aagctcaaac aataatgttt gagctttggt
       61 tttattcaat gacttttgca gtatatttga ggtttccgac cgcttgtatc agggcttggt
      121 tatccacact ttcttttacc accacagtcg cttttcgctc tttcattgag gctttggtgg
      181 aaatcacacc ttctactgct cgtagttctt tattgactaa ataaacgcag agctggcagt
      241 acatttccgg aatatggagg acgacggttt tctctgcttg tttcgtttca atttgagctg
      301 ttttttcttg tgcgtagctt gaatgaacca aaccaaaagc caacagcatc gttgcgatat
      361 attttttcat tttattctcc gctccacatt tccaaaatgt aaggcaatac ataagggtaa
      421 gttaaaaaga acatcaccac cacagaaact acccaataca aaatgaccag ggttctgcgg
      481 gagaagtatt tggtgcagat gattttgttg gaaaaattca gcaaccagaa gccatagcca
      541 aacgcagcga gcgaaatcag caacatcggc agttgtagaa actccagttg gtttagctcc
      601 atcaaccaag gggaagagat gccaaacact aaataaacca gtggcccaat acaacagaat
      661 gtggaggtca ccgccgacac tatggcggtc acgcacattg tgataaattt atggttataa
      721 ctctttagag atgaattcat agccaaattc tttcgcctca aacgagttta acgggtcacc
      781 acctacctca gcataagggt agccaaagtt attcagtggt gtaagttccg gttgtggata
      841 taaatcgaaa tcacgtccgt gattgaagcc tacccagttc atttcccagt tgccgaataa
      901 gtatttcgag accgcttgaa catcagcatc atccacgtct tttttctcgg ctaaacgcat
      961 tttagcgaca tcggcagaat ctacaggcac ccagccgaag cctgctaaat agaactctgc
     1021 acggcagtgc tgcccgccgc tttcattcgc cacgccatct tttgagctac caaatgcggt
     1081 tttggaatat tgccccattt ttaccgcttg ccctaaacga ataccaaaca cttcacgagc
     1141 aggaatgtta gaggcacgag caagtgctac aaacaccgag ttgatgtcgg tacatttacc
     1201 ttttagcact ttggtggtga gaattttctc cacatcgcca tcgccacagc ctagcaccga
     1261 gttatcacgt tccatattat caacaatcca cttgtggata agttccgctt ttttcaacgg
     1321 attttgctcg ttacccacaa ttttatcggc atattctttc acaataccgt ctgttttaat
     1381 gtgagccgtt ggttttaaat actccaatac atcaacagaa taagtgattt tctccggcat
     1441 ttggtaattt gccaacgccc cttgagccat aggctcacga tcttgggttt ggatcaccat
     1501 tttcactttt agatcacgct ttggtgcatc agtatcccaa gtggcaaaca aggtttttgc
     1561 accataagcg ttattttctg tgatataggc gtttttgtaa cttccctcaa actctatcga
     1621 ttttaccgtt tgataatcgg tatcagacgg caacggaatc cacaaattag ttgttccttt
     1681 cgagccttgt ggcacaacta aatcataaga ttgcgtaaat tcgtaagtgt gggtagtggt
     1741 gtggaaattg ttctgttgaa caggagcagc aaaagccgtc gcacagcttg aagccaccaa
     1801 agaggcaata agtttattca ttgaaatgat ccttattaga gtgaaaaaac agttaaaact
     1861 taacatatta agtattctaa ctacggattg aggttgcata ttagccaatc gagatgggaa
     1921 aagcaatggg ggagatgatt ttttagatag tgcaagcgtc cacgcttgta ctagaaacaa
     1981 agctaaaata atcaataggc acaagcgtgg acgcttgcgc catcagagag aataacaagc
     2041 ggtcaaaaat cctcaaaatt ttacaaatag attttttgcc ctaattttgc actttcaaat
     2101 acgctttcga tcacttttag cacaaaaatg acgtcttcga gtttgataat tggctcagca
     2161 ttgtgagcga tggcttggta caaatcatca aacaagtttt gataactgac ttcggcattt
     2221 cgatagggct ggcgaaccac attaccttcc acttcagtgt gtaaaattcc ccagttctcc
     2281 ttttcttcga tattccaatc tttgccaata ggttgtacgc catttttcag caaatcttct
     2341 tgattatcgg gagtttgttt cagataagag cctaattttc cgtgtaatac aaatgtcggg
     2401 cttggttcac gagcatatcg gctggctttc agcaccactt taaaaccgtt agcataatag
     2461 agctgaatat caaagttatc atcaaccaat gccccttcgt gctgataacg cacatcagca
     2521 tacaccgcct ccggcttccc gaaaagatac aaagcttggt caattaaatg tacgcctaaa
     2581 tcatacacta aacctgtacc aagctcgcct gattctttcc atactttaac atttttctct
     2641 ttcgcataac ggtcaaaacg aatttcgcaa tctaccaact ctcccagtaa attttgctcc
     2701 attagcgatt ttgccgtcgc aatatggctg tcccaacggc ggttttggta cacataaagc
     2761 acctttcctt gccgctttgc aagttccgcc aactcttccg cctcagccac tgttgccact
     2821 aacggttttt caactaaaac gtgtttgcct gcaagcaatg actcttttac cattgcataa
     2881 tgggtttgat tcggtgtggt aatcagcact aaatccactt ctggcgttaa aagagcgttg
     2941 aaatcccgca ctgtttctgc attcggcaac cattctgccg cattgttaga ggttcgttca
     3001 tataccttta caatattaaa acgagcatct tgtttcaaaa acgggatatg aaaaactcgg
     3061 ctggaaaagc cggcacctgc gatggcaata ttgatgggtt gcatagacaa tcctcataaa
     3121 attttgatgt tatctgctta aataaacaag cggtcaaatt ttaggaaaat tttgcaaaaa
     3181 acagataaaa tacgaccgct tgtttaacta atcataggtt ttgcaaacaa tatctttttc
     3241 ttcacaaaac atttcactgt ggtttgcacg gcattgcttt tgtacattta atttggctct
     3301 gcctcggcta ctatgttcac tacgatattc ctgagtaaat ggctttagag tacaaatgtg
     3361 aacctctttg gaatcaagtg gatctgagac accaattata atcggcatag ggataccggc
     3421 ttgccccgta ggagtaaccg gcacgacaca gccacttaat agtatgctta aacttattaa
     3481 ccaataacgc atatttttct ccttttaaaa tgtataataa atcagaacgg agcatcttta
     3541 tcgttacagt aagctcttaa tatcttgcct aatttcttgg tatttttatt aaaatttcgg
     3601 catttagggc ataccaaaag atgcaactgc aaacgcatat gttgttgcaa acgaagtttc
     3661 tcatcacaag actgtgaaat aagttctgtt gcttgcctgc atttcatttt gattcctctc
     3721 taaaccaatt gcgagataaa cactcttgta aacgtaaacg agcacgatga agtaatacat
     3781 ttaagttaga gagagaaatt tcacattctt ggcaaatttc cgcactactc atctccaaat
     3841 gttcacgcat cataaatact cgtgcttgct gggcgggtaa tttatttaaa caaatatcaa
     3901 aaattgccca aaattctttt tggtaagttg attgagctac ccccctccat tcagttacat
     3961 cttgatgttc caaccaatga tcgtggcgat cgaaaaaggc acttggtgat tcttcttcct
     4021 cacatagaga actgactgga atatgtcgtt ttcgtgttct gagtaaatct aaaattttgt
     4081 tctttaaaat agcaaaaatc caagttttaa gtgcagcttc tcgccgaaaa gaggctgaat
     4141 gcttataggc attggttaag gcatcctgta caacatcctc agctaaatct ttatcggcta
     4201 gttgtagggt ggcaaattta accatttgct ttctaatctc ttctatctgt gtagaagata
     4261 gactcttcat atatctcctt tagggaatac ccaataaaaa attacactta ttttgtaaga
     4321 attctagatc agctgagtcc aaaataataa caacaattca taaaaaaagg atagttcgat
     4381 gaaaaaatta acaattcaag atgttacacc cgaaactatt ttctaccaac gccgtaaaat
     4441 tattaccgcg ttaggtttag gtattgcagc ttctgcattg cctaaagtaa gtatggcaga
     4501 aacacaaaga cacttgcaat cactacagtt taaaccggat ccaaataaag ggcttgaact
     4561 cactccggaa aacaaagtaa taggctataa caatttctat gagtttggcg tggataaaag
     4621 tgcaccagca aaaaatgcgg caaacttcaa aaccgatcct tggacattgg aaattggcgg
     4681 agaagttgaa aatccgttta gcttaaatca tcaacaattg cttaatacgt ttccactaga
     4741 agaacgcatt taccgttttc gctgtgttga agcgtggtca atggttgtgc cgtggatcgg
     4801 ctttgagtta gctcgcttaa ttgagatggc aaaaccaacc agtaaagcca aatatgtggt
     4861 ctttcacact ttatatgatc ctgcacaaat gccggggcaa aaaaatcgct tgctaggcgg
     4921 tgggattgat tatccttatg tagaagcttt acgcttagat gaagccttaa attcattaac
     4981 actactatcc gtcggtttat acggtaaaac cctgccaccg caaaacggtg ctcctattcg
     5041 cttagtcgtg ccttggaaat atggctttaa aagtattaag tcgattgtga aaatcacctt
     5101 tgctgaaaaa cgcccactca ctacttggga aagtcttgct ccaaatgaat atggctttta
     5161 tgctaatgtg aatccaaatg ttgatcatcc acgttggtcg caagcatctg aacgagtaat
     5221 tggtgcaggc gggttattat ccgtcaaacg ccaaccaaca ctaatgttta atggctacga
     5281 aaatgaagtg gcaaatttat accgtggctt agatttaaag gtgaactact aatgctaacc
     5341 tggttacgta tactcattca tcttggctgt ttattaccgc tagtttgggt agcacagcta
     5401 ttatatacgg gtaatgaaac aatattaggt gccgatccga ttaaagagct ggagcatttt
     5461 ctcggttatg gtgcaatcgt cattttttgt ataatgtttc tacttggtat tgccctacaa
     5521 tacctaaaga aaaaccagta tcaaatttta cgccgtccat tgggcttatg ggcgtttgta
     5581 tgggcagctc tacatatttc aagctatcta ttgttagaat taagccttga tgtaaaatta
     5641 ttttttagcg agcttgccgg tcgcccatat ttgattttag gagcagctgc ttttttcatt
     5701 ctaagcataa tggcaataac ttcgctgcct atgctaaaac gaaaattagg aacacgctgg
     5761 gggacgattc accagtgggc ttatcctgca ttagtattgg cgatggtaca ttattattgg
     5821 tcggtaaaga gcgtaacatt cgatccaatt atcatcggag tcttagtgct gtttattgtt
     5881 gctcataaaa tacaggctaa atttgcaaaa aaatagcaaa atcacaccgc ttgcagcagt
     5941 caaactacat acaaaaaata aggggctgtc ctagataacg actaaaac
//
LOCUS       DANLXS010000147         5980 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.148, whole genome shotgun sequence.
ACCESSION   DANLXS010000147 DANLXS010000000
VERSION     DANLXS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.148"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            109..540
                     /locus_tag="PXD71_002078"
     CDS             109..540
                     /locus_tag="PXD71_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL6292157.1"
                     /translation="MSNQIALLLMSHGSFARAALASAELIVGQQENVAAIGVVAVDNV
                     ADIKRQMEQTIAELNTDKGLIILTDIVGGTPMNLASSQLTHPNVFACSGLNLPLLLEV
                     LMSRDGNLQEIRENLPLAYQNGFSLRTQQDFVSQEDDDDLL"
     gene            527..994
                     /locus_tag="PXD71_002079"
     CDS             527..994
                     /locus_tag="PXD71_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL6292158.1"
                     /translation="MICFSRIDDRLIHGQVVTTWVNLHRIEQIIVLNDAVAADPTQKQ
                     ILTMAAPQGIKVVAFGIEKFGEVLQKTQITRRTMLLFTNSIDVLRTVELGVSIDKLNV
                     GGMRYQARREQLSKAVSVTPEERQAFKTLLEKGVEVTVQMVPNDEKLNLQEII"
     gene            997..1764
                     /locus_tag="PXD71_002080"
     CDS             997..1764
                     /locus_tag="PXD71_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL6292159.1"
                     /translation="MLTALLVACWAGICALDDIGTQMIRRPLLIAPVVGLIMGDLETA
                     LIIGATLEVMWMGIGNVGAYSAPDMISGTAIGTALGIAAGEASTAVALAVPTSLLAQQ
                     LLILYRSGVCALNPIAERWAASGDFDKIFRIHYIPMLIAFSIRAIPTFLAVYYGADII
                     AQAVSALPAKVITGLNVAGKIIPAIGIGLLMLMMIKKAELWTFLIAGFTLAVYLKLSV
                     LPITLIALPLALIYDLAVSKKAEAAPTKTADEEDYDL"
     gene            1761..2567
                     /locus_tag="PXD71_002081"
     CDS             1761..2567
                     /locus_tag="PXD71_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL6292160.1"
                     /translation="MSNKITKQDLNKVFWRMQVLNVTNNFQSMQAIGFLSSFIPVLKR
                     LYKDRPQEERAEAMKRHLKFFNSHVNSDALILGIAAAVEETTKEDEKETVTAIKTGLM
                     GPLAGLGDSLLKFTWLPICGSIGAALALSGNVLGPILMFLLYNSVNIATKYYGIHLGY
                     SKGVDLIHGAGNNILQRLGNMANVVGLMVIGALIASVVKVKVVSQIAAGDNVIKFQEM
                     LDKVMPGLLSLLLTVVIYWILKKTNGKYAATMILATMGICIGLVYAGILG"
     gene            2567..3604
                     /locus_tag="PXD71_002082"
     CDS             2567..3604
                     /locus_tag="PXD71_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDL6292161.1"
                     /translation="MDSVYDIIRLEQGRYLFRQQPAQSVQIFFLDNPDSKDSKWLAET
                     DLAEFELKLSACEARPFFLIQSANGEQIVAERTLPVAGMNNFRDMGGYVAHQGKRVKW
                     GKLYRSDHLHNLRDEGVAYLDKLGIQTVIDYRSPNEVAKYPNPPINGREQTFRLDPNA
                     HTAELAAQFSADKHDEDRNLVNKIIAQKAEGNLINRYDIVMAQYRNFVEKAECQTAFA
                     EMLRLATDPENAPLVQHCRGGKDRTGFGAMLLLGILGVSKADIIADYMLTHYNRLARN
                     EEKMAIYRTFTQDQDVLDYLLSLIDTQPEFIEQSLNTIETQYGTIEQYAQRVLGITAK
                     EIEALRANYLA"
     gene            3694..4914
                     /gene="agp"
                     /locus_tag="PXD71_002083"
                     /pseudo
     CDS             3694..4914
                     /gene="agp"
                     /locus_tag="PXD71_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628146.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
     gene            5222..>5980
                     /locus_tag="PXD71_002084"
     CDS             5222..>5980
                     /locus_tag="PXD71_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6292162.1"
                     /translation="MSQDIQADLKKILALLSRNRRFQSLFLLLLRSEQFINSDALSTE
                     LQLSVRTVKKEIKLLKEKLEPYGIQIFSRPFYGYKLLIQFEELHTQLKQYFQISQPMT
                     VNNKFESRVNAILRRLLVSQRALKVEDFQSELYLSFTSALTKEFSQVKALLGKYGLIL
                     EAKPYHGMNIVGESYRKIMLTVRLYKYFTQLEHPFGIKEFEQCFACPMTEKIRERLAK
                     TIIHFNLVFSDIYAERFIIYLRYFYNQQPELLLPK"
BASE COUNT         1831 a         1100 c         1289 g         1760 t
ORIGIN      
        1 tcactttata tttcttctta tgtgtacgat atatcgtgca ataacttccc ttgtttatct
       61 gtataacatt ttatttaata gaatgactta taaatcaagg agtgacttat gtctaatcaa
      121 attgcgttat tgttgatgag ccacggcagt tttgcgaggg cggcgttggc gagtgccgag
      181 ctgattgtcg ggcagcagga aaatgtggca gctatcggcg tagtagcagt ggataatgta
      241 gcggacatta aacggcagat ggagcaaacc attgctgagt taaacaccga caaaggcttg
      301 attattctca ccgatattgt tggtggtacg ccgatgaatc ttgcctcttc tcaattaacc
      361 caccctaatg tttttgcctg ttcgggtttg aatttacctt tattgctaga agtgctgatg
      421 agccgtgacg gcaatttgca agaaattcgg gaaaatctac cgcttgcata ccaaaacgga
      481 tttagcctac gcacacaaca agattttgtt tcacaggagg acgatgatga tctgctttag
      541 ccgtattgat gaccgtttaa ttcacggtca agtagtcacc acttgggtta atttacaccg
      601 tattgaacaa attattgtgt taaatgatgc ggtggcagcg gatccaaccc aaaaacagat
      661 tttaacgatg gcagcaccgc agggaattaa agtggtcgcg tttggcattg aaaaatttgg
      721 tgaagtgttg cagaagacac aaattacccg ccgtactatg ttgttgttta ccaatagcat
      781 tgatgtgtta cgcactgtag aactaggtgt atcgattgac aaattgaatg tcggcggaat
      841 gcgttatcaa gctaggcgtg agcagttaag taaagcggtt tcagttacgc cagaagagcg
      901 tcaagcattt aaaaccttat tggaaaaggg tgttgaagta acggtacaga tggtaccgaa
      961 tgatgaaaaa cttaacttac aggagattat ctagctatgc tgactgcact acttgttgcc
     1021 tgctgggcag gtatttgtgc cttagatgat attggcacac agatgatccg ccgtccgttg
     1081 ttaatcgcac cggtggtcgg attgattatg ggggatttgg aaacggcgtt gattattggt
     1141 gctacgcttg aagtgatgtg gatggggatc ggcaatgttg gtgcatattc tgcaccggat
     1201 atgatttccg gaaccgctat tggcaccgca cttggtattg ctgctggtga agcttctact
     1261 gcggttgcac tcgcagtacc gacctcactc ttggcacagc agttactcat tctttaccgc
     1321 tccggcgtat gtgcattgaa cccaattgct gaacgctggg cggcaagcgg tgattttgac
     1381 aagattttcc gcattcacta tatcccaatg ttaattgcct tttctattcg tgctattcca
     1441 acgttcttag ccgtctatta cggggcggac attattgccc aagcggtctc tgctctgccg
     1501 gcaaaagtga ttaccggatt aaatgttgca ggcaaaatta ttcctgccat cggtatcggt
     1561 ttgctgatgt taatgatgat taagaaagcc gaactttgga cgttcttaat tgctggcttt
     1621 acgttggcgg tttatctcaa attatcagta ttgccgatta ccttaattgc actaccgctt
     1681 gcgttgattt acgacttagc agtaagcaaa aaagctgaag ctgcaccaac caaaaccgca
     1741 gatgaagagg attatgacct atgagcaata aaattaccaa acaagattta aacaaagtgt
     1801 tttggcggat gcaggtgctg aacgtcacaa acaacttcca atcgatgcag gcgattggat
     1861 ttttaagtag ctttattccg gtattaaaac gactttataa agatcgtcca caggaagaac
     1921 gagcggaagc aatgaaacgc cacttaaaat tttttaacag ccacgttaat tctgatgcat
     1981 tgattttagg aattgccgct gcggtagaag agactaccaa agaggatgaa aaagagacag
     2041 taaccgcgat taaaaccggt ttaatggggc cgttagcagg gctgggagac agcttgttga
     2101 aattcacctg gttaccgatt tgcggtagta tcggggcggc attggcacta agtggcaatg
     2161 tgttaggccc gattttaatg ttcttgttgt ataacagtgt aaacattgcg acgaagtatt
     2221 acggtatcca tctaggttac agcaaagggg ttgatttaat tcacggtgcg ggtaataaca
     2281 ttttgcaacg actcggcaat atggcaaatg tggttggttt aatggtgatt ggcgcattaa
     2341 ttgcctccgt tgtgaaagtc aaagtagttt cacaaattgc cgctggtgat aatgtgatta
     2401 aattccaaga gatgttagat aaagtgatgc cgggactact cagcttactt ttaactgtcg
     2461 tgatctactg gattttgaag aaaactaacg gcaaatatgc tgcaacgatg atccttgcta
     2521 cgatggggat ctgtatcggc ttagtctatg caggaatact cggctaatgg acagtgtgta
     2581 tgacattatt cggttggaac agggcagata cctgttccgc caacagccgg ctcaatcggt
     2641 acagattttc tttctggaca accctgacag taaggatagc aaatggctgg cggagactga
     2701 tttggcagag tttgagctga aattgagcgc ttgtgaagcc cgtccgttct tcctcattca
     2761 atcagcaaac ggagaacaga ttgttgctga acgcacctta cctgttgccg gaatgaataa
     2821 ttttcgggat atggggggct atgtcgctca tcagggcaag cgagtgaaat ggggcaagtt
     2881 ataccgctcc gatcatctgc ataatttaag agacgaaggc gtggcttatc ttgataaatt
     2941 gggaattcag actgttattg actatcgcag cccgaatgaa gtcgccaaat acccaaatcc
     3001 cccaattaat ggcagagagc aaacttttcg gcttgatccg aatgcacata ctgccgagct
     3061 tgctgcccaa tttagtgcag acaagcacga tgaagatcgt aatttggtca ataaaatcat
     3121 tgctcaaaaa gctgaaggta atcttatcaa tcgctacgac attgtaatgg cacaatatcg
     3181 taattttgtc gaaaaagcag aatgccaaac cgcctttgct gaaatgttaa ggttggcgac
     3241 agaccctgaa aatgcaccat tggttcaaca ctgccgtgga gggaaagatc gcactggttt
     3301 cggtgcaatg ttactgcttg gtattttagg cgtgagtaaa gctgatatta ttgctgatta
     3361 tatgcttact cattataacc gccttgctcg taacgaagag aaaatggcga tttaccgtac
     3421 cttcactcaa gatcaagatg tgttggacta cctattgtct ttaatcgata ctcaacccga
     3481 atttatcgaa caatcgctta atactattga aacccaatat ggtactattg agcaatatgc
     3541 ccaaagggta ttaggtatta ctgcgaagga aattgaagca ttacgggcaa attatttggc
     3601 gtagaacggt agggtgtaat attgaacaca ctacaaatgg agaaatttca tatgcgttat
     3661 tttgcaaaac attggtataa aactaccgct tgtctgacaa caattttcac agcatttcct
     3721 ctttttgctc aaccaattga aaatatacca acttcccact acgaattaga aaaaatcatt
     3781 atatttagcc gacacgggtt acgttcccct cttacagaaa aaggcagtga tttagaaaaa
     3841 tcaacccctt ataaatgggc aaattggagt gtaccgaccg gtcatttaac ccataaaggg
     3901 acgctgttgg aaacctattt tgggcagtat cttggtcaat ggctcacaca taatgggtta
     3961 ttggacaagg caagctgtga aagtgggaag cagattgagg tttatgctaa tagcctgcaa
     4021 cgtacaattg gcactgcaca agctttggtt gccggtgggt tttcaggttg taatgtacaa
     4081 gttaaacacc accagcagat tggaaagatg gatccggtgt ttaatccaat tattcgctcc
     4141 gatgaccaga catttgtgca aacagcatta agcaaaattg acctcaataa aacccatcag
     4201 tatttagcgc catcctatca tttattaagc gaaataatcg attatgctca ttcagaaaaa
     4261 tgtttgcagg aaaaacagtg tgaatttttt aatgatgttg gtaaattaag gattaaaaag
     4321 gataaataac caggtgtttc cggtacgatg cgattaagta aaaatattgt cgatgcctta
     4381 ttgctgcaat attatgaagg ttttccttta aaagagattg caaataacca gcttgatagt
     4441 gatcagaaat ggcaaacact caataaaatt aaaaacgatt accaatttat gctacgagga
     4501 gatccattta ttgctaaaca tatttcatta ccgctattaa aatatattca gcaagattta
     4561 aatagtgaaa ataaaataac acttttagtc ggacacgatt ccaatattat agccttactt
     4621 tctgcattaa atgttaagcc ttataaatta gcacatcaat atgaacaaac tccaatagga
     4681 gggaaagtct ttattgaaat ctggaaagaa aaaagtagcc aagcacaaaa agttaaactg
     4741 gaatatgtat atcaatctac tgaccaaatt cgtaaagcga ttccactaag ccttgataaa
     4801 ccaccgcaac atcacatttt gagtttggat tattgccaag ctgaccaata tggattatgc
     4861 gattatatgg aatttaaaca gttactggaa aaagtcattg ctgatacatt gtgagatttt
     4921 gacaaagtga gtgaaaaagg tagcataaaa aagtcgggac agttgccagc tgctccctgt
     4981 aagatgttgt ttacagcact agtaatgcta ctatccccaa aaagctgctg aaaggcagct
     5041 tttttgttgt atataaaagt acacctttct acgcttcatt ctttttctcc aattaaacct
     5101 aatttaggac ttctagtgat aaattcggta tcatatctta attcactaca tatcgtgcaa
     5161 gccttaaaat tgaaagggct gacgaaatcc tatttaattc tgacacacat aagggaatat
     5221 tatgtcgcaa gatattcaag ccgatttaaa aaagatattg gcactattaa gccgtaaccg
     5281 tcgatttcaa tcattatttt tattactgtt aagaagtgaa caatttatta atagtgatgc
     5341 actttctacg gagttacagc tttcagtacg aacggtaaaa aaagagatta agttattaaa
     5401 agaaaaactt gaaccttacg gcattcagat tttttctcga ccgttttatg gctataaatt
     5461 attgattcag tttgaagaat tacacacgca actaaaacag tatttccaaa tatcacagcc
     5521 gatgacggta aataataaat ttgagtctcg ggtaaatgcg attttacgcc gattactggt
     5581 gagccagcgt gcattgaaag ttgaagattt tcagagtgag ctttatttaa gttttaccag
     5641 tgccttaaca aaagaattta gtcaagttaa agccttgtta ggaaaatatg gattgatact
     5701 cgaagcgaaa ccctatcacg gaatgaacat tgtaggtgaa agttatcgta aaataatgct
     5761 cacagtacga ttatataaat attttaccca gcttgaacat ccatttggaa ttaaagaatt
     5821 tgaacagtgt tttgcttgtc ctatgactga aaaaatccgt gaaaggttag ctaagacaat
     5881 tattcatttc aatttagtat tttctgatat ttatgctgag cggtttatta tttacttacg
     5941 ctatttttat aatcaacagc ctgagttatt gttaccgaag
//
LOCUS       DANLXS010000148         5969 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.149, whole genome shotgun sequence.
ACCESSION   DANLXS010000148 DANLXS010000000
VERSION     DANLXS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.149"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..318
                     /locus_tag="PXD71_002085"
     CDS             <1..318
                     /locus_tag="PXD71_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL6292163.1"
                     /translation="LYSAMVNRDFSALQTLLSDDFELYSSTGEVIDKQEWIKLIQKRV
                     LEFDKVDLLSTDFQGNQSSGLLRVNGEFFGIERDNVVVEMNIRTIVRNTERQIKCIVI
                     KEV"
     gene            331..1224
                     /gene="murQ"
                     /locus_tag="PXD71_002086"
     CDS             331..1224
                     /gene="murQ"
                     /locus_tag="PXD71_002086"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL6292164.1"
                     /translation="MNALGQMITEQRNPNSMNIDRLSALDIVQVINQEDKQVAIAVEQ
                     CLPQIAQAVEKIVQAFEKGGRLVYVGAGTSGRLGVLDASECPPTYGVKPEMVVGLIAG
                     GERALRFPIEGAEDNREQGKVDLQAVNFSEKDVLVGIAASGRTPYVLGAMEYAKQLGA
                     TTVSIASNPNSAMAQIADIAIETVVGPEVLTGSSRMKSGTAQKLVLNMLTTASMILIG
                     KCYQNLMVDVQATNEKLKARAIRIVMQATECEREVAEDTLKLAENNAKLAIMMILGNL
                     DKQKAEDLLSQNQGRLQKALK"
     gene            complement(1308..2252)
                     /gene="prmB"
                     /locus_tag="PXD71_002087"
     CDS             complement(1308..2252)
                     /gene="prmB"
                     /locus_tag="PXD71_002087"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL6292165.1"
                     /translation="MFKYNTELLDEISALPIVNDLVTIQDMMRWGYSYFNASDLYYGH
                     GYDNAWDEAQQLVLAGLYLPFDVPETLYSSRLTRVEKERIINLICERLGSRKPVAYLT
                     NSAWFCGGEYYVDERVIVPRSPIGELIKKGFKGIIKNEPHRILDMCTGSGCIAIACAE
                     RFPEAEVDAVDLSLDALDVAQINIERHQVSHRVFPICSDLFNNLPQDKYDLIVTNPPY
                     VDEEDLGDMPEEFHHEPALALGSGKDGLDLTKRILAQAADYLNDDGVLVCEVGNSMVH
                     LIEQFPSVPFNWLEFEHGGLGVFSLTKEELVKHRHLFA"
     gene            2345..2890
                     /gene="smrB"
                     /locus_tag="PXD71_002088"
     CDS             2345..2890
                     /gene="smrB"
                     /locus_tag="PXD71_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDL6292166.1"
                     /translation="MNDTLLDDEFALFREAAKGTKKLTQNTFVPKAEPRKKLQELREL
                     KEKADTEFYFSDEYEPLLKEENEKVKYLREDVDPYILKQLRRGDFQPELFLDLHGLTR
                     EKAKKELAALILACEREKVYCASVMTGFGTRTLKDQIPRWLVQHPKVMALHQAPKEWG
                     GDASILILVEQPENPEKMFGR"
     gene            2954..3439
                     /locus_tag="PXD71_002089"
     CDS             2954..3439
                     /locus_tag="PXD71_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL6292167.1"
                     /translation="MSEQTFHQLTALLDSHRARYRVVGHPSAGKSEEVAKIRGTEIGQ
                     GAKALVCKVKGNGVKQAVLAILPADYQADLTKIAEHLGGLRASLASPAEVAELTDCVF
                     GAIPPFSFHPDLLLVAEPSLFERYAELAFNAGTLERSIILNTEDYQKIVNPVLVQFAK
                     S"
     gene            complement(3441..4190)
                     /locus_tag="PXD71_002090"
     CDS             complement(3441..4190)
                     /locus_tag="PXD71_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL6292168.1"
                     /translation="MEKRFEINEQGLSIRCKLFYEKELQQIENVVIVLHGFGSSKDLK
                     SNTKFGERLSSKYKGYAALAFDFPCHGDDARKKLSVEESITYLQLVIDYAKTSLNAKN
                     LYAYGTSFGGYLTLKYLAEKGNPFRKIALRAPAVQIYHSLMNRLSEEERQKLAKGREI
                     LWGFDRQMKISQAFFDELTDITQNDYLDYAEQILILHGTEDEMVALSTSQQFADNNVI
                     ELLPIEGADHAFSNPKLMDIAIGKIVEFFAP"
     gene            4362..5225
                     /locus_tag="PXD71_002091"
     CDS             4362..5225
                     /locus_tag="PXD71_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HDL6292169.1"
                     /translation="MLQLILAILCSVTVGVLIKFARQKGIRIDQSIAVNYIVTTTMTF
                     LLLKPDYQGQSITDIIANNPTSYIFVILSITLPTIFLVQAKALEFAGIIRTDAAQRLS
                     LFLPILAAFTIFGEQVTQNKLIALLLAFVALGCLLWKGNQGMEKGGKIAIISLALVWV
                     GYGVNDILFKQIAKSGSAFPVTLSIAFIGAGCFMFIYLLLKRTQWHVPSVLTGLLLGV
                     LNFGNILFYIQAHQAMKDDPTLVFTGMNLGVICLGTFVGAFFFKEKIHKVNYLGVAIA
                     IVAIICLFYWR"
     gene            5349..5942
                     /locus_tag="PXD71_002092"
     CDS             5349..5942
                     /locus_tag="PXD71_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292170.1"
                     /translation="MKLKALSTLIASSMLYSQSFAVPPEKTAEEPQPFFNKTESGYDL
                     VFNDQNYRLIETELNGEKIQFRAFERIVYVNNPIEPDYQTINLYVPEAYYNNGEINGY
                     QADTAPIFLPNSVGGYMPAKAASYDKKRRDGSPGTILVALSKGYVVASVGARGRTLQK
                     EGKYTGKAPSAIIDSKSAAHKACRFESDHWHHSLCKI"
BASE COUNT         1790 a         1163 c         1346 g         1670 t
ORIGIN      
        1 ctttatagtg caatggtgaa ccgtgatttt tcggctttgc aaacattact aagcgatgat
       61 tttgagcttt attcatcaac aggtgaagtg attgataagc aagaatggat caaattaatc
      121 caaaagagag tattggaatt tgataaggtt gatttgctat ctaccgattt tcaggggaat
      181 caatcatctg gattattaag agtaaatggt gagttttttg ggatagagcg ggataatgtg
      241 gtggtagaaa tgaatattcg aaccattgtg agaaatacag aacgccaaat taaatgtatt
      301 gtgattaaag aggtttaagt gtcgcaacta ttgaatgcct tagggcagat gattaccgaa
      361 caacgtaatc cgaattcgat gaatattgac cgcctttcag cgttagatat agtccaagtc
      421 atcaaccaag aagacaaaca agtagcgatt gcggtggagc agtgcctgcc tcaaattgca
      481 caagcggtgg aaaaaattgt gcaagccttt gagaaaggtg gacgattggt ttatgtcggt
      541 gccggcacga gcggaagatt aggggtgctt gatgcttcgg aatgtccgcc cacttatggc
      601 gtaaaacctg aaatggtggt tggattaatt gccggtggtg agcgggcatt acgtttcccg
      661 attgaagggg cggaagataa tcgtgagcaa ggcaaagtag atttacaagc ggtcaatttt
      721 agcgaaaaag atgtattggt cgggattgca gccagtgggc gaacgcctta tgtgttaggg
      781 gcgatggaat atgctaagca acttggtgcg accacggtgt cgattgccag taacccaaat
      841 tcggcaatgg cacaaattgc ggatattgcg attgaaaccg ttgtcgggcc ggaagtgcta
      901 accggttcca gccgtatgaa atccggcaca gctcaaaaat tggtattgaa tatgctcact
      961 actgcgtcga tgattttaat cggtaaatgc taccaaaatt taatggtaga tgtgcaagca
     1021 actaatgaaa aactcaaagc tagagcaatt cggattgtga tgcaagcgac agaatgtgaa
     1081 cgagaagtgg ctgaagatac actaaaatta gccgaaaata acgctaaatt ggcaattatg
     1141 atgattttag gtaacttaga taagcagaaa gccgaagatt tattaagcca aaatcaaggg
     1201 cgattacaaa aagcgttaaa atagaagtat gtctgctctc cccttgtggg agagagacag
     1261 atcaatcatt cattcagaat gattgatcag agagagggga ggcagattta agcaaataaa
     1321 tggcgatgtt tcactagttc ttctttggtt aaactgaaca cccctaagcc accgtgttca
     1381 aattccagcc agttaaacgg cacactgggg aattgttcga ttaaatgcac catactgttg
     1441 ccgacttcgc agactagcac gccatcatca tttaagtaat cagcggcttg agcaagaatc
     1501 cgcttggtta aatctaaccc atctttgccc gagccaagag ctaatgccgg ctcgtgatgg
     1561 aactcttcgg gcatatcgcc taagtcttct tcatcaacat aaggcggatt agtcacaatt
     1621 aagtcgtatt tatcttgcgg taaattgtta aacagatcgg aacagatcgg gaaaactcgg
     1681 tgagacacct gatgacgctc aatattaatt tgagcaacgt ctaaggcatc aagggaaaga
     1741 tcaaccgcat cgacttctgc ttccggaaaa cgctcggcac aggcaatcgc aatacaaccg
     1801 ctgccggtac acatatctaa aattcgatgt ggctcgtttt tgataatgcc tttaaaccct
     1861 tttttgatca gctcaccaat cggtgaacgt ggcacgatca cacgctcatc gacataatat
     1921 tcaccgccac aaaaccaagc cgagttggta aggtatgcta caggtttgcg agagcctaaa
     1981 cgctcacaga ttaaattgat gatacgttct ttctcaactc gagttaagcg agaggaataa
     2041 agtgtttccg gtacatcaaa cggcaaataa agccctgcca acactaattg ttgtgcctcg
     2101 tcccacgcat tatcgtagcc gtgcccgtaa taaagatcag aggcattaaa atagctatac
     2161 ccccaacgca tcatatcttg aatagtgact aaatcattca caatgggcag ggcggaaatt
     2221 tcatcaagaa gttcggtatt atatttaaac attggcgtgc cttttttcgt attgttaaaa
     2281 tggctgtgtt ataccacatt ttgctttgtg ttagaatgta aaaatgaata agaaggaaaa
     2341 gataatgaac gatacgttat tagacgatga atttgcatta ttcagagagg cagcaaaagg
     2401 cacgaaaaag ctgacacaaa atacctttgt gccgaaagcg gaacctcgca agaaattgca
     2461 agaattgcga gaattaaaag aaaaagctga tacagaattt tatttctcgg atgaatatga
     2521 gcctttgctg aaagaagaaa atgaaaaagt gaaatatctg cgagaagatg ttgatcctta
     2581 tattctcaaa cagttacggc gaggcgattt ccaacccgag ctgtttttag atctgcatgg
     2641 tttaactcga gaaaaagcga agaaagagtt ggcagcgttg attttagcct gtgagcggga
     2701 aaaggtatat tgtgccagtg tgatgaccgg ctttggcact cgcacgctca aagatcaaat
     2761 tccacgctgg ttggttcagc acccaaaagt aatggcgttg caccaggccc cgaaagagtg
     2821 gggcggagat gcctctattc taattttggt tgagcagcct gaaaatcctg aaaaaatgtt
     2881 tgggcgttaa aattcggctg aattgccaat tttttattaa aaacgaccgc ttgtgtatca
     2941 ttaaggagaa atgatgtcag aacaaacttt ccaccaactt acagcattat tagattcgca
     3001 tcgagctcgc tatcgagtgg taggacatcc ttctgccggg aaatcggaag aagtggcaaa
     3061 aattcgagga acagaaatcg ggcaaggggc gaaagcctta gtttgcaagg taaaaggcaa
     3121 tggggtaaag caagctgtgc ttgctatatt acctgctgat taccaagcgg atttaacaaa
     3181 aattgcagag catcttggtg ggttaagagc ctcacttgcc agcccggctg aagtggcaga
     3241 attgaccgat tgtgtatttg gggcaattcc gccgtttagc tttcaccccg atttattatt
     3301 ggtggctgag ccaagtctgt ttgagcgtta tgctgaatta gcctttaatg ccggtacgtt
     3361 ggaacgttcg attattctga ataccgagga ttaccaaaag atagttaatc ctgtgttggt
     3421 tcaattcgcc aaaagctagg ttatggggcg aaaaactcca cgattttgcc aatagcgata
     3481 tccattaatt tagggttaga aaaagcgtga tctgcccctt caatcgggag cagttcaatc
     3541 acgttattat cggcaaactg ctgtgaagtg cttaacgcca ccatttcatc ttccgtgccg
     3601 tgcagaatta aaatttgctc ggcataatcc aaataatcgt tttgggtaat atcggttagc
     3661 tcatcaaaaa aggcttggct gattttcatc tggcgatcaa acccccacaa aatttctcgc
     3721 cctttggcaa gtttttgccg ttcctcttct gacaagcggt tcattaacga atggtagatt
     3781 tgtactgccg gtgcccgcaa tgcaattttc cgaaacggat tgcctttttc cgccagatat
     3841 ttcaaggtta aatagccgcc aaaactagtg ccgtaggcat acagattttt agcattcagg
     3901 ctggttttgg cataatcaat caccagttgc agataagtaa tggattcctc aaccgagagt
     3961 tttttacggg catcatcacc gtggcaaggg aaatcaaagg caagggcagc gtagccttta
     4021 tattttgagc taaggcgttc cccaaatttg gtattcgatt tcagatcttt agacgaacca
     4081 aagccgtgca acacaatcac tacattctcg atctgctgca attctttttc ataaaacagt
     4141 ttacagcgaa tgctgagacc ctgttcatta atctcaaagc gtttttccat tttatgttaa
     4201 gtattgaaat tgaagatgac aaaattttag cactatttga gtgcaagcgg tcggatctct
     4261 cgcaaaattt gcaaattttc cggtaaattt aaccgcttgt actagccaaa atttaagcat
     4321 atttatagaa tacagccttc gtttttaaaa ggagaaatga aatgttacaa cttattttag
     4381 cgattttatg tagcgtgact gtaggcgtgt tgattaaatt cgcccgccaa aaagggattc
     4441 gtatcgacca aagtattgcg gtgaactata tcgtgactac cacgatgacg tttttattac
     4501 taaaaccgga ttaccaaggg cagtcgatta ccgatattat tgctaataac ccgacttcct
     4561 atatttttgt gattttaagt atcacattgc ctactatttt cttggtgcaa gcaaaagccc
     4621 tagagtttgc cggtattatc cgaaccgatg cggcacaacg tttatcgctg ttcctgccga
     4681 ttttggcggc attcaccatt tttggtgagc aggttacgca aaataaatta attgcacttc
     4741 tgttagcttt cgtggcactc ggttgcttat tatggaaagg caatcaggga atggaaaaag
     4801 gcggaaaaat tgcgattatc agccttgctc tggtttgggt aggctatggc gtgaacgata
     4861 ttttatttaa acagattgcc aaatcaggct ctgctttccc tgtcaccctt tcgattgcct
     4921 ttatcggtgc aggctgtttt atgtttatct atttgctact taaacgcact caatggcacg
     4981 ttccaagtgt cttaaccggc ttattgctag gcgtgttgaa cttcggcaat attctgttct
     5041 atattcaagc tcaccaagcc atgaaagatg acccaacttt agtcttcact ggaatgaact
     5101 taggggtaat ttgcttaggc actttcgttg gagcattctt ctttaaagag aaaatccaca
     5161 aagtgaacta cttaggcgtg gcgattgcga ttgtggcgat tatttgcttg ttttattggc
     5221 ggtaaatata ctttccaaaa ttaagggcga acataatgtt cgcccttaat ttttgagtaa
     5281 tatagtgtaa aatattcaat aaattcgacc gcttgttgta ccgattcata tttcataaag
     5341 gacaaaaaat gaagctaaaa gccttatcta cactcattgc ctcttcaatg ctatatagtc
     5401 agagttttgc tgtaccgccg gaaaaaacag ctgaagagcc acagcccttt ttcaataaaa
     5461 ccgaatctgg gtatgattta gtctttaatg atcaaaacta tcgtttaatt gaaacggaat
     5521 taaatggcga gaaaatccaa ttccgtgcct ttgagcggat tgtgtatgtg aacaatccga
     5581 ttgagccaga ttaccaaaca attaatctgt atgtgccgga agcttactac aataatggcg
     5641 aaatcaatgg ctaccaagcg gataccgccc cgatcttctt gccaaattca gtggggggct
     5701 atatgccggc aaaagcggca agttatgata aaaaaagacg tgacggttca ccaggtacga
     5761 ttttggtggc gttatctaaa ggctatgtgg tggcaagtgt gggagcaaga ggacgcacgc
     5821 tacaaaaaga gggaaaatac accggcaaag caccttctgc cattattgat tcaaaatccg
     5881 ccgcccataa agcgtgtcgg ttcgagtccg accactggca ccactcacta tgtaaaattt
     5941 agttttacat agtttgctgt aagcatttg
//
LOCUS       DANLXS010000149         5962 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.150, whole genome shotgun sequence.
ACCESSION   DANLXS010000149 DANLXS010000000
VERSION     DANLXS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.150"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            6..3077
                     /gene="fdnG"
                     /locus_tag="PXD71_002093"
     CDS             6..3077
                     /gene="fdnG"
                     /locus_tag="PXD71_002093"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:591..593,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDL6292171.1"
                     /translation="MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAK
                     ETRQTCTYCAVGCGMIMYSLGGEAMNSKGKLIHIEGDSDHPVSRGALCPKGAGALDYV
                     NSDRRVHYPEVREAGSSEWKRISWHEAIERIARHMKNDRDENFVTHNEAGLEVNRWMT
                     AGFLAGSACSNETGIVTQKFIRSLGMIFTDNQASIUHGPTVASLAPSFGRGAMTNHWV
                     DIKNSDLVIVMGGNAAEAHPVGFRWAVEAKKQNGAKLMVVDPRFNRSAAVADIYIPLR
                     PGTDIAFLLGAVNYLLKNDKIQHEYVKHYTNATFLLNEGFKFEDGLFTGYDEATRTYK
                     DKSTWAYQLDEQGQPKRDMTMQDPRCVINMLKDHVSRYTPEMVERITGTPQKDFLIFC
                     EEIAKTSAPDKAATFLYALGWTQHTVGAQNIRSMAIIQLLCGNIGVSGGGVNALRGHS
                     NVQGITDLGLFPHMLPGYIRLPTEADATLESYLNAIAPKTLVAGQVNYGQNNPKFAVS
                     MLKTFYGDKATKENEFGYHYLPKYPKGGTDHFRFIEQMYQGKVNGYFCQGMNPIASYP
                     NSQKLIKALSNLKYLVIFDPLITDTSEFWKNHGEFNNVNPSEIQTEVFRLPTTCFAEE
                     DGSIANSGRWLQWHWKAADAPHEAKPDVDILSEIRAELIHLYHKEGGKAPLEPLEAMT
                     WNYMNPLEPKPEEIAKENNGYALEDLKDADGNIIVKKGELLSSFTQMRDDGTTSGGCW
                     IYCGQWTPKGNQMANRDNSDPSGLGNTLGWAFSWPVNRRILYNRASADLSGKPWNPKR
                     QLVKWNGKNWNYVDVADYGTAPPNSPVTPFIMQPEGVSGLFVRERMQDGPFPEHYEPF
                     ETPIGTNPLHPNVISSPVARILPSDKEDLGTSDEFPYVGTTYRLTEHFHYWTKNALIN
                     VIAQPEQFVEIGEKLAEEKGIKNGDKVRVWSKRGEIKAVAVVTKRIRSLVSDGKPIHT
                     IGVPIHWGFAATTGAKKGYFANNLTVRTGEPNSNTPESKCMLLNIEKIVGA"
     gene            3080..3994
                     /gene="fdxH"
                     /locus_tag="PXD71_002094"
     CDS             3080..3994
                     /gene="fdxH"
                     /locus_tag="PXD71_002094"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HDL6292172.1"
                     /translation="MSTVQTQNIIKISATSALTPAPQARSHQVEVAKLIDVTTCIGCK
                     ACQVGCSEWNDIRSDVNANCVGVYDNPADLNAKAWTVMRFNEVEENDRLEWLIRKDGC
                     MHCAEPGCLKSCPSPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPDDNRVY
                     KCTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMKFYAEQRIADLKSRGYENAGLYDP
                     EGVGGTHVMYVLHHADKPELYSGLPKDPKIDTTVTLWKDVLKPVAAVALGGLALAEVA
                     HYITVGPNVEEDLEEHHHSENAEGGKHE"
     gene            3987..4664
                     /locus_tag="PXD71_002095"
     CDS             3987..4664
                     /locus_tag="PXD71_002095"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDL6292173.1"
                     /translation="MSKKFEITNDTKIVRHKPLARVSHWFLVISFFMTMFTGVAFFFP
                     DFAWLTEILGTPQIARAVHPFTGIIMFIAFIIMALIYWHHNIPEKNDIRWAKGIVEVL
                     KGNEHKVSDNGKYNLGQKLLFWTLILAMFTLLATGIIMWRQFFSHYFSIPMLRIAILL
                     HSASAFMLFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELERQ
                     VAEQNNK"
     gene            4720..5637
                     /gene="fdhE"
                     /locus_tag="PXD71_002096"
     CDS             4720..5637
                     /gene="fdhE"
                     /locus_tag="PXD71_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDL6292174.1"
                     /translation="MSIKILPQDDIKQAASSFQQPELLFANPKNLYLRRAKRLRELAK
                     QNPFGEYMEFSANLVDIQLALLESQPIADYSQKITACVERTQGETPLNAQNFQRTDEW
                     RTLLLAIIEKFKPYANDTVLATIEVLEKAAKSELDTLADNLLNERYELVGADKAVFIW
                     AALSLYWVQLAQQLPRNSRAEIGHKHLCPVCNSAPVSSVIHFGEAQGLRYLHCSLCES
                     EWHVVRTKCTNCDETGKLDYWSLDTVEAAVKAESCGDCHSYLKVLYQDKDVNVEPVAD
                     DLATLFLDSEMEQKGLSRSGINPFLFQVE"
     gene            complement(5707..>5962)
                     /locus_tag="PXD71_002097"
     CDS             complement(5707..>5962)
                     /locus_tag="PXD71_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292175.1"
                     /translation="NKEKLNGVKKQQDKPKFRISERKVLKNKEKDEEVSNFSMEELRV
                     GAQRPLDDSVIQYFKSQCRYAFMSDREIIANRCEVKKIAD"
BASE COUNT         1742 a         1298 c         1361 g         1561 t
ORIGIN      
        1 tgattatgca gatctcaaga cgtaagttct tcaaggtctg tgcaggtggt atggcgggaa
       61 cttctgctgc aatgttgggt tttgctccag caacagcatt agctgcaccg cgtgagtata
      121 agctcttaag agcaaaagaa actcgacaaa cctgtactta ctgcgcggta ggctgtggca
      181 tgattatgta cagcttaggc ggcgaagcaa tgaacagcaa aggaaagtta attcatattg
      241 agggtgattc tgaccatcca gtaagccgtg gggcgttatg tccgaaaggt gcaggggcgt
      301 tggactatgt taatagtgac cgccgtgtgc attatccgga agtgcgtgaa gccggttcaa
      361 gtgagtggaa acgcatttct tggcacgagg caattgaacg tattgcccgc catatgaaaa
      421 atgaccgtga tgagaacttc gtcacccaca atgaagcagg gctggaagta aaccgttgga
      481 tgacggcagg tttcttggca ggctcggcgt gtagtaacga aaccggtatc gtcacccaga
      541 agtttatccg ctctctcggt atgattttta ccgacaacca agcgagtatc tgacacggac
      601 caacggtagc aagtcttgct ccatcatttg gtcgcggtgc gatgaccaac cactgggttg
      661 atattaaaaa ctccgattta gtgatcgtta tgggcggtaa cgcagccgaa gctcaccctg
      721 tgggtttccg ttgggcagtt gaagcgaaaa aacagaacgg tgcgaagtta atggtggtag
      781 atccacgctt taaccgtagt gctgcggtgg cggatattta tataccattg cgtccgggta
      841 cggacattgc gttcttgtta ggtgcggtaa actacctgct taaaaacgac aaaattcaac
      901 acgagtatgt gaagcattac accaatgcca cattcttact caacgaaggg tttaaattcg
      961 aagacgggct tttcaccggc tacgatgaag ccactcgtac ctataaagac aaatccactt
     1021 gggcttatca gttagatgaa cagggtcagc cgaaacggga tatgacaatg caagatccac
     1081 gttgtgtgat caatatgctc aaagaccacg tttcccgcta tacgcctgaa atggtagaac
     1141 gcattaccgg tacgccacaa aaagatttcc tgattttctg tgaagagatt gcgaaaacct
     1201 cagcgccgga taaagccgcc accttcctct atgccttagg ttggacacaa cacaccgtgg
     1261 gcgcacaaaa cattcgctca atggcgatca ttcagttgct gtgcggtaac attggtgtct
     1321 ccggcggtgg ggtaaacgca ctgcgtggtc actctaatgt acaagggatt accgacctag
     1381 gcttgttccc acatatgttg ccgggctata tccgcctgcc aaccgaagcg gacgcaacct
     1441 tagaaagcta cttaaatgcg attgcaccga aaaccttagt ggcagggcaa gtaaactacg
     1501 ggcaaaacaa cccgaaattc gctgtgagta tgctcaaaac cttctacggc gataaggcaa
     1561 ccaaagagaa cgagttcggc tatcactatt taccgaaata cccgaaaggc ggtacagatc
     1621 acttccgctt tatcgaacag atgtatcaag gcaaagtgaa cggctatttc tgccaaggta
     1681 tgaacccgat tgcctcttac cctaactcgc agaagttaat taaagcgttg agcaatttga
     1741 aatatttagt catttttgac ccgcttatta ccgatacttc cgagttctgg aaaaatcacg
     1801 gtgagttcaa caatgtgaac ccgtctgaaa ttcaaaccga agtgttccgc ttgccgacca
     1861 cttgctttgc ggaagaagat ggttcaattg cgaactccgg tcgctggtta caatggcact
     1921 ggaaagcagc agacgcacca cacgaggcaa aaccggacgt ggatattctg tctgaaatcc
     1981 gtgccgaact gatccacctt tatcataaag aaggcggaaa agcaccgctt gaaccgttag
     2041 aagcgatgac gtggaactat atgaacccgt tagaaccgaa gccggaagag atcgccaaag
     2101 agaacaacgg ttatgcattg gaagatctga aagatgcaga cggcaacatc atcgtgaaaa
     2161 aaggcgaact attatccagc tttacacaaa tgcgtgatga cggtacaacc tccggtggtt
     2221 gttggatcta ctgcggtcaa tggactccga aaggtaacca aatggcgaac cgtgataact
     2281 ccgacccatc aggcttaggt aacaccttag gctgggcatt ctcgtggccg gtaaaccgcc
     2341 gtattctata caaccgtgca tcggcagatt taagcggtaa accgtggaac ccgaaacgcc
     2401 agctagttaa atggaacggt aaaaactgga actatgtgga cgttgccgat tacggtacag
     2461 caccaccaaa tagccctgtg acaccattta ttatgcaacc ggaaggggta tccggcttgt
     2521 tcgtgcgtga acgtatgcaa gacggtccat tccctgagca ttatgaaccg tttgaaacgc
     2581 caatcggcac gaacccgtta cacccgaatg taatcagcag tccggtggca cgcattctgc
     2641 cgagcgataa agaggattta ggcaccagcg atgagttccc atatgtcggt acaacttacc
     2701 gcttaaccga acacttccac tactggacga aaaatgccct gattaacgtc attgctcaac
     2761 cggagcaatt cgtggaaatc ggcgagaaac ttgccgaaga gaaaggcatt aaaaatggcg
     2821 acaaagtacg tgtttggtct aaacgtggcg aaattaaagc cgttgccgtg gtgactaaac
     2881 gtatccgttc attggtctct gacggcaaac caatccacac tatcggtgtg ccgatccact
     2941 ggggctttgc ggctacaacc ggtgcgaaaa aaggctattt tgccaataac ttaaccgtgc
     3001 gaacaggtga accaaactca aatacacctg aatcgaaatg tatgttatta aacattgaga
     3061 aaattgtggg ggcttaacaa tgagtacagt tcaaactcaa aacattatta aaatctccgc
     3121 aacctcggcg ctgacaccgg cgccgcaggc tcgttcgcac caagtggaag tagcgaaact
     3181 gatcgatgtc accacttgta tcggctgtaa agcctgtcaa gtgggctgtt cagaatggaa
     3241 tgacattcgc tccgatgtaa atgcaaactg tgtcggcgtg tacgacaacc ctgcggatct
     3301 aaatgcgaaa gcgtggacag taatgcgctt taacgaagta gaagaaaacg accgtttaga
     3361 atggttgatc cgtaaagacg gctgtatgca ctgtgccgag ccgggttgct tgaaatcttg
     3421 cccgtcaccg ggtgcgatta tccaatacgc caacggtatt gttgatttcc aatccgacaa
     3481 atgtatcggt tgtggctact gtatcgcagg ttgtccgttc aacattcctc gtatgaatcc
     3541 ggacgataac cgtgtttaca aatgcaccct gtgtgtggat cgtgtcaatg ttggtcaaga
     3601 accggcttgc gtgaaaacct gcccgaccgg tgcaatccgc ttcggtagca aagaagagat
     3661 gaagttctat gcagaacaac gcattgctga tctgaaatct cgtggctatg aaaatgccgg
     3721 cttgtacgac cctgaaggcg ttggcggtac gcacgtaatg tatgtattac atcatgccga
     3781 caaaccggag ctttattcag gcttaccgaa agatccgaag attgatacca cagtaacctt
     3841 atggaaagac gttttaaaac ctgtggcggc agtagcgttg ggtggtttag ctttagcaga
     3901 agttgcacac tatattaccg ttgggccaaa cgttgaggaa gatcttgaag aacaccacca
     3961 tagcgaaaat gcagaaggag gcaaacatga gtaagaaatt tgaaatcacg aacgatacca
     4021 aaatcgttcg tcataagccc ttagctcgtg ttagccactg gttcttagta atctcttttt
     4081 ttatgacgat gtttaccggt gttgccttct tcttccctga ctttgcttgg cttaccgaga
     4141 ttttaggtac tccacagatt gcccgtgcag tccacccatt taccgggatt ataatgttta
     4201 ttgcatttat tattatggcg ttaatatatt ggcaccataa tatccctgag aaaaatgata
     4261 ttcgttgggc aaaaggcatt gttgaagtac taaaaggtaa tgagcataag gtttccgata
     4321 atggaaaata caatttgggt caaaaattac tcttttggac tcttattctt gcgatgttca
     4381 ctcttttagc cacagggatt attatgtgga gacaattttt ctctcactac ttctcgattc
     4441 ctatgctaag aattgcaatt ttacttcatt cagcaagtgc atttatgcta tttaccggaa
     4501 ttttagtaca tatgtatatg gcattctggg taaaaggctc aatcagaggt atggttgagg
     4561 gttgggttac cgttcgctgg gcaaaaaaac accacccaag atggtaccgt gaagaagtgc
     4621 tacctgaatt agaaagacaa gtagctgagc aaaataataa ataacctcaa gtgcggtatt
     4681 attaccgcac ttttatttta ttggtttaaa aagaatatta tgagtatcaa aatactacct
     4741 caagatgata tcaaacaagc agctagttca tttcagcaac ccgagctctt atttgctaat
     4801 cccaaaaatc tgtatttacg ccgcgctaag cggctacgtg aattagccaa gcaaaaccca
     4861 ttcggtgaat atatggagtt ttctgctaac ctcgtggaca ttcaattagc attacttgaa
     4921 agccagccaa ttgcagatta ttctcaaaaa ataaccgctt gtgtagagcg cacgcaagga
     4981 gaaacaccgc taaatgcaca gaattttcag cgtactgatg aatggcggac actactctta
     5041 gccattattg agaaattcaa gccttatgca aatgatactg ttcttgcaac aattgaagta
     5101 ttggaaaagg cagctaagtc agagctagac acacttgctg ataatttgct aaatgaacga
     5161 tacgaacttg tgggcgcaga taaagctgtc tttatttggg ctgctctctc actttactgg
     5221 gtacaattag ctcaacaatt accacgtaat tctcgtgcag aaataggcca taaacattta
     5281 tgtccggtat gtaactcagc acctgtaagc agtgtgattc atttcggtga agctcaaggg
     5341 ttacgttatc tacactgttc tttatgtgaa agtgaatggc atgtagtacg tacaaagtgt
     5401 acaaactgtg atgaaaccgg caaactggat tattggagct tagatacagt agaagcagca
     5461 gttaaagctg aaagttgtgg cgactgccat tcctacttaa aagtactcta tcaagataaa
     5521 gacgtgaatg tggaacctgt tgctgatgat ttagcaactc tattcttaga ttccgagatg
     5581 gaacaaaaag gtctttcaag aagtgggata aatccatttt tatttcaagt agagtaactc
     5641 atttacaaca aaaacggagc ataacgctcc gttttttatt acatttaact catacactat
     5701 acttttttaa tcagctattt ttttaacttc acagcggtta gcaataatct cccgatctga
     5761 cataaatgcg tatcggcatt gagatttaaa atattgaatc acagaatcat caagaggtcg
     5821 ttgtgctcca acacgtaatt cctccataga aaaattacta acctcttcat ctttctcttt
     5881 atttttcaat accttacgtt cagatattct aaacttaggc ttgtcttgct gtttttttac
     5941 tccgtttaat ttttctttat ta
//
LOCUS       DANLXS010000150         5955 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.151, whole genome shotgun sequence.
ACCESSION   DANLXS010000150 DANLXS010000000
VERSION     DANLXS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.151"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(117..1481)
                     /gene="mpl"
                     /locus_tag="PXD71_002098"
     CDS             complement(117..1481)
                     /gene="mpl"
                     /locus_tag="PXD71_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDL6292176.1"
                     /translation="MKKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFL
                     AAHGIEIIPNYDVAQLQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLL
                     KDKWVLAVSGTHGKTTTTGMLAWILDQNGIDTGFLIGGVAGNFGISARAGNSPFFVIE
                     ADEYDSAFFDKRSKFVHYTPKTLIINNMEFDHADIFDDLKAIQRQFHHLVRTMPQSGC
                     ILSAIADQNVQDTLKMGSYSQLQFIGEQNEWFAKPLNADCSQFEVFHLGVKQGEVHWS
                     IIGKHNMHNALMAIAAAHHAGVEVQGACEALGKFVNANRRLEVKGEVNGITVYDDFAH
                     HPTAIEATIDALRGKVGKNQRILAVLEPRSNTMKMGVHKDEIAPSLHDAEAVFVYQPD
                     IIAWSVSEITNVLSQPAYWSANIDDLVDLVVKEAKPTDHILVMSNGAFGGIHQKLLAK
                     LADK"
     gene            complement(1777..2532)
                     /locus_tag="PXD71_002099"
     CDS             complement(1777..2532)
                     /locus_tag="PXD71_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HDL6292177.1"
                     /translation="MKLKSFKPMFMLLSVAFLTACSSSINPETGVRDDPLEGFNRAMW
                     KVNYDYLDPYVLKPVAKGWKEYVPSPVKTGLINVANNLDEPVSFVNRLIEGEGKKAMV
                     HFNRFWINTVFGLGGLIDWASLDEGLRLNNGQRGLGESLGAHGVPSGSYIMMPAYGAT
                     TPRQGIGSAVETGYSVLTYVGSPWSIAKFVVQGVDTRSKLLEQDALLEQAQDPYVTFR
                     EAYFQNLEFKVKDGNVEQSQQEQLSEEELKNID"
     gene            2687..3004
                     /gene="metJ"
                     /locus_tag="PXD71_002100"
     CDS             2687..3004
                     /gene="metJ"
                     /locus_tag="PXD71_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDL6292178.1"
                     /translation="MADWNGEYISPYAEHGKKSEQVKKVTVSIPIKVLEILTNERTRR
                     QIKNLRHATNSELLCEAFLHAFTGQPLPNDEDLMKDRADEIPEAAKQKMCELGIDPDE
                     WQY"
     gene            3052..3282
                     /locus_tag="PXD71_002101"
     CDS             3052..3282
                     /locus_tag="PXD71_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HDL6292179.1"
                     /translation="MYQQLTTEARWAVLLTLIYLLGWVGFAYFSPQGRGIFGFPIWFE
                     LSCIFLPVLFSLVATIVVKKVYKNIDLEVNNE"
     gene            3275..4699
                     /gene="panF"
                     /locus_tag="PXD71_002102"
     CDS             3275..4699
                     /gene="panF"
                     /locus_tag="PXD71_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDL6292180.1"
                     /translation="MNSEMILPLIAYLIFVFGVAFYAYRKRQGGSFISEYYIGGRSMS
                     GFVLAMTTAATYVGASSFIGGPGAAYKYGLGWVLLAMIQVPAVLLSLGALGKKFALLA
                     RKHNSVTINDMLLARYQNKFVVWIAGLALLLSFFAMMTVQFIGAGRLLETTLGIPYQT
                     AVMIFAITVGLYTFIGGFRAVVLTDTIQGLVMIVGTSILLAGVIYATGGVENAMHTLE
                     AINPQLTTPYGIDERPLDFTFMTSFWVLVCFGLIGLPHIAVRSMACKNSQALHRAMII
                     GTVVIAILMFGMHLSGVLGRAVVPDLKVPDQVIPTLMVQVLPPVVAGIFLAAPMAAIM
                     SSIDSMLIQSSSTLIKDLYLAIKPNAINNEKKIKLFSTITTLTFTVLLVFAALTPPEM
                     LIWLNLLSLGGLEATFLWVIVLGLYWEKANATGAIYSMLAGLSCYVLMTTFKIAIFSF
                     HAIVPSLLIGLIAFLIGNRFAPKV"
     gene            complement(4776..>5955)
                     /gene="nhaA"
                     /locus_tag="PXD71_002103"
     CDS             complement(4776..>5955)
                     /gene="nhaA"
                     /locus_tag="PXD71_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDL6292181.1"
                     /translation="KEFLKSEASGGVLLLVFAFAAIVCANTSLSDFYFEFLQTIVSVQ
                     FGEFALNKPLLLWVNDGLMAVFFTLVGLEVKKELFEGSLSSYKKAIFPAIAAVGGMIV
                     PAIIYWAINQNHPEYHNGWAIPMATDIAFAVGIVALLGTRVPLALKAFLLALAIIDDL
                     GAIVVIAIFYSQEISVQALTIAAIAIIGLVLLNRFKVTSLCAYLVVGLVLWTSVLKSG
                     VHATLAGVIIGFCVPLKGKHGERPAEELEHILAPWCAYMILPIFAFANAGVYLLEMGM
                     EQLSSPLPLGIALGLIIGKTLGVFGFSYLAVLFRLASLPQGVNFKQIFAIAVLCGIGF
                     TMSMFLASLAFDVSEAGESITALARLGILLGSAVSAILGYTLLRMATKQNNPENNATY
                     "
BASE COUNT         1712 a         1349 c         1215 g         1679 t
ORIGIN      
        1 ataaaacatg tataaatatc cagaattgat tttgaatttt tcgatgcaga tcacaaaaat
       61 agagagttat acaaacaagc ggtaaaattt tgtaaaaaat ttaccgcttg taaaggttac
      121 ttatcagcta atttggcaag tagtttttga tgaataccgc caaaggctcc gttgctcatc
      181 actaagatgt gatcggtggg tttggcttct ttgacgacta gatcgactaa atcatcaata
      241 tttgccgacc aatacgccgg ttgcgataac acattggtaa tctcgctcac actccacgca
      301 ataatatcag gctggtaaac aaacactgct tcggcatcgt gtaggcttgg ggctatttca
      361 tctttatgga cacccatttt cattgtattt gaacgaggct ctaacactgc caaaatgcgt
      421 tgatttttgc cgactttacc acgcagagca tcaatagtcg cttctatcgc agttgggtgg
      481 tgagcgaaat catcataaac ggtaatgccg tttacctcgc ctttcacttc caaacggcgg
      541 ttagcattca caaacttgcc taatgcctcg caagcccctt gcacttcaac ccctgcgtga
      601 tgagcggcgg caatcgccat cagagcatta tgcatattgt gcttgccgat aatcgaccaa
      661 tgcacctcgc cttgtttaac gcctaagtga aacacctcaa actgtgagca atcggcattt
      721 aacggtttag caaaccattc gttctgttcg ccaataaatt gaagctggct gtaactgccc
      781 atttttagtg tatcttgcac gttttgatca gcgattgccg acaaaataca gccactttgt
      841 ggcatcgtgc gaacaaggtg gtggaactgg cgttgaatcg cttttaaatc atcaaaaata
      901 tcggcgtggt caaattccat attattgata atcagcgttt tcggggtgta atgcacaaat
      961 ttagaacgct tatcaaagaa cgcagaatcg tactcgtccg cctcaatcac gaagaatgga
     1021 ctgttaccgg cacgagccga aatgccgaaa ttaccggcaa caccgccaat taaaaagccg
     1081 gtatcaatcc cattttggtc taaaatccac gctaacattc ctgttgtggt agttttgccg
     1141 tgcgtgccgg aaaccgccag cacccatttg tctttaagca aatgatcgtg caaccattgc
     1201 gggccggagg tgtaattgag ttggttgtcc aacacatatt ccacgcacgg attaccacgg
     1261 ctcatcgcat taccaatcac cactaaatca ggagccggtt gcagttgagc aacatcgtaa
     1321 ttcgggataa tttcaatacc gtgagcagct aaaaaggtac tcatcggtgg gtaaacatta
     1381 gtgtctgagc cggtcacttt atagccaagc tcacgggcaa tcattgccac accgcccata
     1441 aaggtgccgc aaatgcctaa aatatggata tgttttttca tctttttata tttaaaatgt
     1501 tagaatcatc ccctctcccc ttgtgggaga gggatagatt ttttcttcgt gcagaagaaa
     1561 aaatcaggga gaggggtaag cggtcatttt taggcttaat attgtaagct ccctctctct
     1621 gaaaaatatt tctgaacgaa atatttttcg gtctcaatac tcgctacgct cgctcctgtg
     1681 gagccgttgg caaagccaac gttcaaatgt aaacatttgt cccacaaggg gagaggtgcg
     1741 ctttagatga ggcgtaccat ccctcgttca catcaattaa tcaatattct tcaactcttc
     1801 ttccgaaagc tgctcttgtt ggctttgctc aacattgcca tctttcactt taaattcaag
     1861 gttttggaaa taagcttcac ggaaggtaac ataagggtct tgagcttgct ctaacaacgc
     1921 atcttgttct aataatttag aacgggtatc taccccttgt accacaaatt tagcgataga
     1981 ccaaggtgac ccaacataag tcagcaccga ataaccggtt tccaccgcac taccaatacc
     2041 ttgacgaggt gtggttgctc cataagccgg catcataata taggagccgg aaggcacgcc
     2101 gtgagcgcct aaactctcac ctaaaccacg ttgtccgtta tttaaacgta acccctcatc
     2161 taaactcgcc cagtcaatta acccacctaa accaaacaca gtgttgatcc agaagcggtt
     2221 gaagtgaacc atcgcttttt taccttctcc ttcaattaag cggtttacaa agctcaccgg
     2281 ctcatcaagg ttattagcaa cattaattaa gcctgtttta accgggcttg gtacatactc
     2341 tttccaacct ttagctaccg gttttaacac ataagggtct aaatagtcgt aattgacttt
     2401 ccacatcgca cggttaaacc cctcaagtgg gtcgtctcgc acgccggttt ctgggttaat
     2461 gcttgatgaa caagcggtta aaaatgccac actgagtagc ataaacatcg gtttgaacga
     2521 cttgagtttc ataaattgaa ccttttgcag agaaatttgt ccattgtagc gaagttgata
     2581 aaaaatacaa aacaaaaagc cgttttgcct ctagaaatct agacgtctag attgagatat
     2641 agttcgcttt tgattacaat acaggcaaca tttaaggagc aaacaaatgg cagactggaa
     2701 cggcgaatat attagccctt atgccgaaca cggcaaaaaa agtgaacaag tcaaaaaagt
     2761 gacggtatcc attccaatta aagtattgga aattttaact aatgagcgaa cccgtcgcca
     2821 aatcaaaaat cttcgtcacg caaccaacag cgaattattg tgcgaggctt ttttgcacgc
     2881 ctttaccggt cagcctttgc cgaatgatga agacttgatg aaagacaggg cagatgaaat
     2941 ccctgaagcg gcaaaacaaa aaatgtgtga gctgggaata gacccggacg aatggcaata
     3001 ttagacacac cttgcaactg cgaggttctt tttatgtttt aaggagactt tatgtatcaa
     3061 caattaacaa cagaagcacg ttgggctgtg ttgcttacgc tgatttatct gctcggctgg
     3121 gtaggttttg cttacttttc accgcaaggc agaggcattt tcggctttcc gatttggttt
     3181 gaactttctt gcattttctt acccgtacta tttagcttag ttgccaccat tgtcgtcaaa
     3241 aaagtgtata aaaatatcga tcttgaggtg aataatgaat agtgaaatga ttttacccct
     3301 gattgcttat ttaatttttg tgtttggcgt agcgttttat gcttaccgta aacgccaagg
     3361 cggaagcttt atttcggaat actatatcgg tgggcgttca atgtcgggct ttgtgttagc
     3421 aatgacaacc gccgccactt atgtgggggc aagttccttt atcggtgggc cgggtgctgc
     3481 ctataaatac ggtttaggct gggtgttact ggcaatgatt caagtgcccg cagtgctact
     3541 ctctcttgga gcattaggca aaaaatttgc cttacttgcc cgcaagcata atagtgtcac
     3601 cattaacgat atgttattag cccgttacca aaacaaattt gtggtttgga ttgcaggact
     3661 agccttatta ctttcgtttt ttgcgatgat gacggtgcaa tttatcggtg ccggtcgctt
     3721 acttgaaacc acactcggaa tcccttacca aacagcggta atgatttttg ccatcacagt
     3781 cgggctttat acctttatcg gcggttttag agcggtggtg ctaaccgata ctattcaagg
     3841 tttagtaatg atagtcggca caagcatttt actcgctggt gtgatttatg ccacaggcgg
     3901 agtggaaaat gcaatgcaca cccttgaagc cattaaccca caacttacca ccccttacgg
     3961 cattgacgaa cgcccgcttg atttcacctt tatgacttca ttttgggtat tggtttgctt
     4021 tggtttaatc ggcttaccgc atattgcggt cagaagtatg gcatgcaaaa acagccaagc
     4081 cttgcaccga gcaatgatta tcggcacggt agtcattgca attctaatgt ttggaatgca
     4141 cctttcaggc gtattaggca gagcggttgt gcctgatctc aaagtgccgg atcaagtcat
     4201 tccaacacta atggtacaag tgctaccacc ggtggttgcc ggtattttcc ttgccgcccc
     4261 aatggcagcg attatgtctt cgattgactc aatgttgatt cagtcgtctt caaccttaat
     4321 taaagatctc tatctggcaa ttaagccgaa tgccattaat aacgagaaaa aaatcaaatt
     4381 gttctccact attactactc ttacttttac ggtattactc gtgtttgccg ccctaacccc
     4441 gcctgagatg ttaatttggc tgaacctact ctctttaggt ggtttagaag cgactttcct
     4501 atgggtaatt gtactagggt tgtattggga aaaggcaaat gccaccggtg ctatttactc
     4561 aatgctggca ggcttaagtt gttatgtatt aatgactacc tttaagattg ctatttttag
     4621 cttccacgcc attgtgcctt cactgctaat tggtttgatt gctttcttaa tcggcaaccg
     4681 atttgctcct aaggtgtaaa ataaaatagg catagtggta tggaagttca ctatgcctat
     4741 tttgcaaaat ttccgctcaa ttcaaccgct tgtagttaat aagtggcatt attttctgga
     4801 ttattctgct tagtcgccat tctaagtaac gtataaccta aaatcgctga aaccgcagag
     4861 ccgagtaaaa tacctaatcg agctagagcg gtaatacttt ccccagcctc acttacatca
     4921 aaggcaagac ttgctaagaa catcgacatt gtaaaaccga taccgcatag caccgcaatg
     4981 gcaaaaattt gcttaaaatt aaccccttgc ggcaggctgg caaggcggaa taatactgct
     5041 aaataactaa aaccgaatac acctaaggtt ttgccgataa ttaagccgag cgcaatacct
     5101 aacggcaatg gtgatgagag ttgctccatt cccatttcta ataagtaaac gccggcatta
     5161 gcgaaggcga agatcggtaa aatcatataa gcacaccaag gggcaagaat atgttctagc
     5221 tcctctgccg gtcgttcccc atgtttacct ttcagcggta cgcagaagcc gatgatcacg
     5281 ccggcaaggg tagcgtgaac ccctgatttt aaaacggaag tccatagcac taagcctact
     5341 accaaataag cacagagtga ggtcacttta aagcggttaa gtagcactaa gccgataatc
     5401 gcaatcgcgg caatagttaa ggcttgcaca ctgatttctt gcgaatagaa aatggcgatt
     5461 accacaatcg ctcctagatc atcaataatc gctaatgcca acaaaaatgc ctttaaagcc
     5521 aatggcacac gagtgcctaa taacgccaca atgcccaccg caaaggcaat atcagttgcc
     5581 attggaattg cccaaccatt atgatattca gggtggtttt gattaatcgc ccaataaata
     5641 atcgctggaa caatcatacc acctactgct gctatcgcag gaaaaatagc tttcttataa
     5701 cttgaaagcg aaccttcaaa caactctttt tttacttcca gccccaccaa cgtaaaaaac
     5761 actgccatta agccatcgtt aacccagagt aatagcggtt tattcaaggc aaattcacca
     5821 aactgtacac taacaatcgt ttgtaaaaac tcaaaataga aatcacttaa ggaggtgttg
     5881 gcgcacacaa ttgctgcaaa agcaaaaacc agaagcaata cgcctcctga ggcttcagat
     5941 tttaaaaatt ctttt
//
LOCUS       DANLXS010000151         5946 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.152, whole genome shotgun sequence.
ACCESSION   DANLXS010000151 DANLXS010000000
VERSION     DANLXS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.152"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(128..1414)
                     /gene="purD"
                     /locus_tag="PXD71_002104"
     CDS             complement(128..1414)
                     /gene="purD"
                     /locus_tag="PXD71_002104"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL6292182.1"
                     /translation="MNILIIGNGGREHALAWKVRQSPLADKVFVAPGNAGTAMENGIE
                     NVAISATDVPALVTFAKQNSVGLTIVGPEAPLVVGVVDAFRAHGLTIFGPTQAAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
                     LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRV
                     GEGDSGLNTGGMGAYSPAPVVTKAIHQRVMDEIIYPTVQGMASENTPYTGFLYAGLMI
                     MPNGQPKVIEFNCRFGDPETQPIMMRLESDLVELCLKACEGKLDSVQSKWCEQAALGI
                     VLAAAGYPGDYRKGDEIIGIPTSTSNQKVFLAGVEQKEGKLLTNGGRVLCATALGNSV
                     FEAQQQALQLAEQIQWQGKFYRRDIGYRAVARKQAK"
     gene            1648..2022
                     /gene="rpsF"
                     /locus_tag="PXD71_002105"
     CDS             1648..2022
                     /gene="rpsF"
                     /locus_tag="PXD71_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL6292183.1"
                     /translation="MRHYEIVFMVHPDQSEQVPAMIERYTASVKEAGGQVHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQSVIDELETNFRYNDAVLRNLIVHTKAAVTEASPM
                     VKAKESKVAEAVAEVESEEAGE"
     gene            1997..2371
                     /gene="priB"
                     /locus_tag="PXD71_002106"
     CDS             1997..2371
                     /gene="priB"
                     /locus_tag="PXD71_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDL6292184.1"
                     /translation="MNQRKQASNLPIDNCLILSGTVSSLVKQSLNPLGVPHYSFWLEH
                     RSIQTEVNLERQAWCKIQVVLAGNQFSYLTQQIKLGESVRIKGFIHTHKDFNGLNQLV
                     LHTEQIEFIDQEKPNGTLFPSS"
     gene            2346..2573
                     /gene="rpsR"
                     /locus_tag="PXD71_002107"
     CDS             2346..2573
                     /gene="rpsR"
                     /locus_tag="PXD71_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL6292185.1"
                     /translation="MARYFRRRKFCRFTAENVVEIDYKDIATLKNYISESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLALLPYTDNHQ"
     gene            2589..3038
                     /gene="rplI"
                     /locus_tag="PXD71_002108"
     CDS             2589..3038
                     /gene="rplI"
                     /locus_tag="PXD71_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL6292186.1"
                     /translation="MQVILLDKVAHLGSVGDQVTVKSGFARNFLIPQGKAVMATAANI
                     AHFEARRAELEAKAAAALATAQERAAKIAALAAVSVSATAGDDGRLFGSVSAKDIAEA
                     LTAAGVGVAKSEVRLGEGPLRTTGEHQVKIHLHPEVNATVAVNVVAE"
     gene            complement(3102..3878)
                     /gene="dnaQ"
                     /locus_tag="PXD71_002109"
     CDS             complement(3102..3878)
                     /gene="dnaQ"
                     /locus_tag="PXD71_002109"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDL6292187.1"
                     /translation="MTEEKIIRQVVLDTETTGMNMAGGPPQIGHNIIEIGAVEVINRR
                     LTGRTYHVYIKPPREVDEEAIAVHGITNEFLQDKPVFAEIAEEFLDFIKGAELIIHNA
                     PFDVAFMDQEFSYLPNPPAKTAEMCTVTDSLQMARRMYPGKRNNLDALCDRLGIDNSK
                     RVLHGALLDAEILADVFLMMTGGQISLLGEEETEVVSEVIDIEEEFSAIVLNADEAII
                     LKANDEEEESHLALLKLIEKKSKGNCLWTKALTDTAEISH"
     gene            complement(3966..5636)
                     /gene="ettA"
                     /locus_tag="PXD71_002110"
     CDS             complement(3966..5636)
                     /gene="ettA"
                     /locus_tag="PXD71_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDL6292188.1"
                     /translation="MSTQFVYTMHRVGKVVPPKRHILKDISLSFFPGAKIGVLGLNGA
                     GKSTLLRIMAGVDKEFEGEARPQPGIKIGYLPQEPKLAPQQTVREAVEEAVAEVKNAL
                     TQLDEVYAMYADENADFDKLAAKQAELEAIIQAHDGHNLQNQLERAADALRLPDWDAK
                     IEHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITH
                     DRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKEKRLAQEQAAESARQKSIEKELEW
                     VRQNPKGRQAKSKARMARFEELNSGEYQKRNETNELFIPPGPRLGDKVIEVQNLTKSY
                     GDRTLIDDLSFAIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIVLGETVVMASV
                     DQFRDAMDDKKTVWEEVSNGQDILTIGNFEIPSRAYVGRFNFKGVDQQKRVGELSGGE
                     RGRLHLAKLLQRGGNVLLLDEPTNDLDVETLRALENAILEFPGCAMVISHDRWFLDRI
                     ATHILDYGDEGKVTFYEGNFSDYEEWKKKTFGEAATQPHRVKYKRIAK"
BASE COUNT         1563 a         1379 c         1264 g         1740 t
ORIGIN      
        1 gaaaagagtt tactgcatcg tttaatgata gtgatttaat ttcggggcct gcatcgagtt
       61 cagtgatatt aatttcatta agtgttgtac tcataataaa tttttccttg ttttaagagt
      121 ttataaatta tttcgcttgt tttcttgcca ctgctcggta gccaatatct cggcgataga
      181 atttgccttg ccattgaatt tgttcggcaa gttgtaaggc ttgttgttga gcttcaaata
      241 cactgttgcc aagtgcggtg gcacaaagta cacgtccacc gttggtgagc agttttcctt
      301 ctttttgctc aacccctgct aagaaaactt tttgattgga ggtagaggtc ggaatgccta
      361 tgatttcatc gcctttacgg taatcgcccg ggtagcctgc ggcagctaaa acaatgccta
      421 atgcggcttg ttcacaccat tttgattgca cgctatccaa tttgccttcg cacgctttta
      481 agcacaattc aacgaggtct gattccaaac gcatcataat cggctgagtt tcagggtctc
      541 caaaacggca gttaaattca atcactttcg gctgaccgtt tggcataatc attaagccgg
      601 catagagaaa accggtgtaa ggggtatttt cgcttgccat tccttgcacg gtcgggtaaa
      661 taatttcgtc cattacacgt tggtggattg cctttgttac caccggagca ggggagtaag
      721 cccccattcc gccggtattt aagccgctat cgccctcgcc aacacgtttg tggtcttgtg
      781 aggttgccat tggttcgaca tttttgccgt ccaccatcac aataaaactg gcctcttctc
      841 cgtctaaaaa ttcctcgatc accacacggc tgccggcttc accaaaggcg ttgcctgaca
      901 gcatatcacg cactgcatct tccgcctctt gtaaagtcat tgcaacaatt acgcctttgc
      961 ctgcggctaa accgtctgct ttgattacaa tcggtgcacc tttttgtttt aagtaggcaa
     1021 gtgccggttc aatttcggtg aaattttggt attctgcggt tggaatctga tggcgagcca
     1081 agaaatcttt ggtaaatgct ttagaacctt ctaattgagc agcggcttgg gtcgggccga
     1141 aaatggttaa accgtgggct cgaaaagcat caaccacgcc aactactaac ggtgcttcag
     1201 ggccgacaat cgtcaaacct acgctatttt gtttagcgaa tgtaactaag gcaggcacgt
     1261 cagtggcgga aatagctaca ttttcaatgc cgttttccat cgctgtgccg gcattgccgg
     1321 gggcgacaaa gactttgtct gcgagcggcg attgacgaac tttccaagca agagcgtgtt
     1381 cacgcccacc gttgccgata attaaaatat tcattgggat tccttgcaaa aatgataaag
     1441 caaacgtttg cgtattgtac ttgatttgtt tatctgctga caagcagtcg tttttctcgg
     1501 attttttgca aaatacgaaa ttgttgcttg caaaataggc ttttttcagt ataatcctgc
     1561 cgttcagtca cattcgtttt ttaatccttg cttccacaag ttggtgactg gctaaagttg
     1621 gaggctgcta acccgtaagg atagtcaatg cgtcactacg aaatcgtttt tatggttcac
     1681 ccggaccaaa gcgaacaagt accagcaatg attgagcgct acacagcgtc tgtaaaagaa
     1741 gctggcggtc aagttcatcg cttagaagat tggggtcgtc gtcaattagc atacccaatc
     1801 aacaaacttc acaaagcaca ctatgtgtta atgaatgtag aagcacctca aagtgtaatc
     1861 gacgagttag aaactaactt ccgttataac gatgcagttc ttcgtaactt aatcgttcac
     1921 actaaagcgg cagtaactga agcgtcacca atggtgaaag cgaaagaaag caaagttgct
     1981 gaagcggtag ctgaagttga atcagaggaa gcaggcgagt aatttgccga ttgataattg
     2041 tttaatctta agcggaacgg tttccagctt ggttaaacaa agcctaaacc cccttggtgt
     2101 gcctcattac agtttttggt tagaacatcg ttcaatccaa accgaagtga atttagaacg
     2161 ccaagcgtgg tgcaaaatcc aagtggtttt ggcaggcaat caatttagtt atttaaccca
     2221 acaaattaag ctcggtgaga gcgtgagaat taaaggtttt atccatactc acaaagactt
     2281 taacggttta aatcagttag tcttacacac cgaacaaatt gaatttatag atcaggagaa
     2341 gccaaatggc acgttatttc cgtcgtcgta agttctgccg tttcacagcg gaaaatgttg
     2401 ttgaaatcga ttacaaagat atcgctacat taaagaacta catttctgaa agcggcaaga
     2461 ttgttcctag ccgtattact ggtactcgtg cgaagtatca acgtcaatta gctcgtgcaa
     2521 ttaaacgcgc tcgctacctt gcgttacttc catatactga taaccaccaa taatcaggag
     2581 agagtacaat gcaagttatt ttattagaca aagttgctca cttaggttct gtgggcgacc
     2641 aagtaaccgt taaatctggt tttgctcgta acttcttaat cccacaaggt aaagcggtta
     2701 tggcaactgc ggcaaacatt gctcactttg aagcacgtcg tgcagaatta gaagcaaaag
     2761 ctgcagcagc attagcaact gcacaagaac gtgcagcgaa aattgcagca ttagcagcag
     2821 tatcagtatc tgcaactgca ggtgatgatg gtcgtttatt cggttctgta tcagctaaag
     2881 acatcgctga agcattaact gcagcaggtg ttggagttgc taaatctgaa gttcgtttag
     2941 gtgaaggtcc acttcgtaca accggcgaac accaagttaa aattcactta caccctgaag
     3001 tgaatgcaac tgtagcagta aacgttgttg ctgaataatc agtagaaata gaaaacccag
     3061 cgaaagctgg gttttttgtt tgctttattt attggttaga atcagtgaga tatttctgct
     3121 gtgtcggtta atgcttttgt ccataggcaa tttcctttgg attttttctc aatcagtttc
     3181 agaagtgcta aatgcgactc ttcctcttca tcgtttgctt taagaataat tgcctcatcg
     3241 gcatttaaca caattgcaga aaattcttct tcaatatcaa taacttcact taccacttca
     3301 gtttcttctt cgcctaataa tgaaatctga ccgccggtca tcattaggaa tacatcagct
     3361 aagatctccg catccagtaa cgccccgtgt agtacacgct tgctattatc aatgccgagc
     3421 ctgtcgcaaa gtgcatctaa attattccgt ttgccggggt acattcttcg tgccatttgc
     3481 aggctatcgg ttacggtaca catttctgcg gtttttgccg gtggattagg cagataagaa
     3541 aattcttgat ccataaacgc cacatcgaag ggggcattgt gaataattaa ttccgcacct
     3601 ttgatgaaat ccaaaaactc ctcggcaatt tcagcaaaaa caggtttatc ttgcaaaaat
     3661 tcattggtga tgccgtgaac tgcaatcgcc tcttcatcta cctcacgcgg tggcttgata
     3721 taaacgtgat aagttctgcc ggttaaacgg cggttaatga cttcaaccgc accgatctca
     3781 ataatattat gcccaatctg tggcgggcca cctgccatat tcattcctgt ggtttcagta
     3841 tccagcacta cttggcggat aattttttct tctgtcatat tcaatttaaa attgtagggg
     3901 tgaaatattt ttatccctta attctgcatt tgattagggg cgaaacatat ttcgccccta
     3961 cattgttact tcgcaatccg tttatatttc actcggtgcg gttgggtggc ggcttcaccg
     4021 aaggttttct tcttccactc ttcatagtcg gagaagttac cctcgtagaa cgtcactttg
     4081 ccttcatcgc cgtagtctaa aatatgggtg gcgatacggt ctaagaacca gcggtcgtgc
     4141 gaaattacca ttgcacagcc cgggaactct aagatcgcat tttccaacgc acgcagggtt
     4201 tctacgtcta agtcgttggt cggctcgtcc agtaacaaca cgttaccgcc acgttgtagt
     4261 agcttcgcca agtgtaaacg accacgttca ccgcctgaaa gctcgcccac acgtttttgc
     4321 tgatctacgc ctttgaagtt gaaacgcccc acataagcac ggcttgggat ttcaaagtta
     4381 ccaatcgtga gaatatcctg cccgttcgac acctcttccc acacggtttt cttatcgtcc
     4441 atcgcatcac ggaactgatc gaccgatgcc attaccaccg tttcaccaag cacgattgaa
     4501 ccagaatccg gctgttcttt accagaaagc atacggaaca aggttgattt acccgcacca
     4561 tttgccccga taatcccgac aatcgccccc ttcggaatag cgaacgacaa gtcgtcaatc
     4621 aaagtgcgat cgccgtaaga tttagtcagg ttttgtacct cgatcacttt atcacctaag
     4681 cgtggacccg gtggaataaa gagttcgtta gtctcgttac gtttttgata ttcgcctgag
     4741 ttaagctctt caaagcgtgc catacgagcc ttacttttcg cttggcgacc ttttggattt
     4801 tggcgtaccc attccaactc tttctcaatc gatttttgac gagccgattc cgccgcttgc
     4861 tcttgtgcca agcgtttttc tttctgctcc aaccaagaag agtagttacc ttcccaagga
     4921 atgccttcac cacggtcaag ctctaagatc cagcccgcaa cgttgtctaa gaagtaacgg
     4981 tcgtgggtaa ttgccactac ggtgccttcg tagtcgtgga ggaaacgctc caaccacgcc
     5041 accgattccg catctaagtg gttggtcggc tcatctaaca ataacatatc cggtttttcc
     5101 agcaacagac ggcaaagtgc cacacggcgg cgttcacccc ctgataaatg ctcgattttt
     5161 gcgtcccaat ccggcagacg taacgcatcg gcagcacgct ctaactggtt ttgaaggtta
     5221 tggccatcgt gggcttgaat aatcgcctct aattccgcct gttttgccgc taatttgtcg
     5281 aaatcggcat tctcatcagc atacatcgca tagacttcat caagctgggt taaggcgttt
     5341 ttcacttccg caaccgcttc ttccaccgct tcacgcacgg tttgctgtgg ggcaagtttc
     5401 ggttcttgtg gaagatagcc gattttaatt cccggttgtg gacgagcctc gccctcgaac
     5461 tctttatcta cgcccgccat aatgcgtaaa agcgttgatt tacccgcccc gtttaagccg
     5521 agcaccccga ttttcgcccc cgggaaaaag ctcagggaaa tatctttaag aatgtgacgc
     5581 ttcggcggca cgactttgcc gacacggtgc atcgtatata caaattgagt tgacattggt
     5641 tgtattcctc acaaaaaaat tcgccttatt ttacttgaat ttacagccaa aatatagggt
     5701 ggttttggct tttggtaaag taaccttttc caatagagat gttatttgag gctgcatagt
     5761 actatgcatt tctcttacct tgacaggtaa gagaaacaaa tcctgaagga aaaaaatttt
     5821 aaggaagtta ttctatgcaa tatgtatgtc caaaaacact caaattattc aacagcattg
     5881 ttggttgtta taagggagaa gattaattct ctacctcatc aaggcttgat tttagattta
     5941 atatcc
//
LOCUS       DANLXS010000152         5894 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.153, whole genome shotgun sequence.
ACCESSION   DANLXS010000152 DANLXS010000000
VERSION     DANLXS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.153"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..810
                     /gene="djlA"
                     /locus_tag="PXD71_002111"
     CDS             <1..810
                     /gene="djlA"
                     /locus_tag="PXD71_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDL6292189.1"
                     /translation="FHGLFGGILGAFIGHLADKKLYELGSVRSSLFGKNLTRQSLFTQ
                     TTFAVLGHIAKAKGRVTEADIELARALMARLKLDSAAQQLAQNAFTLGKEPDFPLRQV
                     IQEFREACGARTDLLRFFVEVQMQAALQDGQLDVNEQQILFTIAETMGMSRFQFEQMI
                     AMIMAAQQFRNGGYYQQNGSYHQQSQGGYQGSYQQPAQPSIEAAYTVLGVTAQDDQNT
                     VKRAYRKLMNEHHPDKLAAKGLPDEMMELAKEKAQQIQAAYDLICKVKEWK"
     gene            810..1436
                     /locus_tag="PXD71_002112"
     CDS             810..1436
                     /locus_tag="PXD71_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDL6292190.1"
                     /translation="MDSRSHIIPLFLAIVITVIAVWSGLNPTDRAVWYAEVIPIFIVF
                     ALLIITYPKFQFSGLAYFLMSLWLIMHLIGAKYTFANVPFEWANQFLTPILGEDRNHF
                     DRVAHYIIGFYSFPMAEWLLRKRKCGMGVAFFFSLFFIMSVAAAYEIIEWQYAVIEGG
                     NAGVEFLGSQGDIWDAQKDMLADTLGALTALMIYLIARPDLRIHDRHF"
     gene            1514..2467
                     /gene="dusC"
                     /locus_tag="PXD71_002113"
     CDS             1514..2467
                     /gene="dusC"
                     /locus_tag="PXD71_002113"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDL6292191.1"
                     /translation="MNPIPNVILAPMQGVLDPFVRNLLTSVNDYDLCISEFVRVVDQR
                     LPKKTFYRLAPELYQGGLTDSGTPVRVQLLGQHPQWLAENAALAIELGSHGVDLNCGC
                     PSKTVNGSNGGASLLKDPELIYRATKAMREAVPSDKIVSVKVRLGWDSVSQCFEIADA
                     VEQGGANEITVHGRTKVDGYRAERINWQAIGEIQQRLSIPVIANGEIWDFESAKNCQK
                     ITACKALMMGRGALNTPNLSKVVKYNVPKMAWNEVLQLLFKYVQMENQFDTGFYHVAR
                     IKQWLRYLDKEYPQAVELFDILKTEHGYDGLKAHIEKAVEE"
     gene            2467..3513
                     /locus_tag="PXD71_002114"
     CDS             2467..3513
                     /locus_tag="PXD71_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDL6292192.1"
                     /translation="MSKQIFNEEELKTTEHFEPKQEFDLENAIIEEEEKAVEAELIIE
                     ESLKPSRFWIRVLLATLALFGVAVIAQSIQWLIDTFQQREWIYFAFSIVFFIVSLLGV
                     GAIISEWRKLVYLRNHQKNQQMSNQLLLEQLPSNDGEQAVKFCENIANQLNHLPSIAQ
                     AEQLWKSELNEAYNAKEVLYLFSENVLKPIDKQVKQMISRNAAENAVIVAVSPLAIVD
                     VLLMAARNIALVNKITKAYGMELGYISRLKLFKMVLKNMVFAGATEIATDVGMDFFSQ
                     NLTAKLSLRAAQGIGVGLLTARLGIKAMEFCRPVAFQANEKPRISAIRQELLTSVKNT
                     VFAKTETKEKVFSD"
     gene            complement(3550..4209)
                     /locus_tag="PXD71_002115"
     CDS             complement(3550..4209)
                     /locus_tag="PXD71_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL6292193.1"
                     /translation="MAVTIYLVRHGRTVWNLEGRLQGSGDSALVEEGIIGAKKTGIAL
                     KHIPFTAAYSSMQKRAQDTANYILAENERSNIPHFHHKGLNEFDFGSWEGMKSVDLQE
                     NDEYWVMKRTPAEYLAKANGGERFEQLYQRVTQAFNQIAELHQNDGKVLIVSHGMTLT
                     LLTAVLKGIAWQDFRNEEKHSFVFNTAITKVEVENGKVTVLEFNNTDHLDNVPTSQYK
                     R"
     gene            4258..4911
                     /locus_tag="PXD71_002116"
     CDS             4258..4911
                     /locus_tag="PXD71_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HDL6292194.1"
                     /translation="MTPTLPICYQHRDFIIINKPEGLSVHKDEAEIGLTELVARQLNV
                     KQVWLVHRLDKITSGLLILALNKEAAARFYHLFEQHKIYKTYWALSDKKPKKKQGRIV
                     GDMQKSRNGAWKLCHSKENPAVTQFISHSVEDSLRHFILQPKTGKTHQLRVAMKSLGS
                     PILGDKLYSGSQADRVYLHAYQLDFEYENEKISVQALPTSGHFWQKICKSNHIKHGE"
     gene            4913..5533
                     /locus_tag="PXD71_002117"
     CDS             4913..5533
                     /locus_tag="PXD71_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDL6292195.1"
                     /translation="MKLWYSTTSPFARKALAVIKHHQLDDKVEMLRVTKAMDPNSPHN
                     QDNPLGRIPALQRNCGRWLFGSLLISEYLDQKGNNTPLLPKEGKPRWAVLALHNLADG
                     IMENTMPTLLQERMARPENEWWVSRHEQLMERNIRSFRQLEEALQEFGTELNLGTLTA
                     VALIDWWAFRLDKIGYDLAKNYPNLTAWAEEMNNKYAILAETKPSM"
     gene            complement(5580..5837)
                     /locus_tag="PXD71_002118"
     CDS             complement(5580..5837)
                     /locus_tag="PXD71_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6292196.1"
                     /translation="MKNLRLSIHSSEHIWLRELFLKRRLELGLTQRTLGEKMGVLYSF
                     IGKVETGDRRLDIFEFIAYCKGLDLDPLSVLQEIEQQFPHK"
BASE COUNT         1732 a         1157 c         1316 g         1689 t
ORIGIN      
        1 tttcacggtt tatttggtgg gatacttggc gcttttatcg ggcatttagc cgataaaaaa
       61 ctctatgaat taggttcagt tcgttcaagt cttttcggta aaaatttaac tcgccaatcg
      121 cttttcaccc aaaccacttt tgcggtgttg gggcatattg cgaaagcaaa agggcgtgtt
      181 actgaagcgg atattgaatt agcccgagcg ttaatggctc gcttaaaatt agatagtgca
      241 gcccagcaat tagcgcaaaa tgcctttacc ttaggtaaag agcctgactt cccgttacgc
      301 caagtcattc aagagttcag agaagcctgt ggggcaagaa ctgacttatt acgtttcttt
      361 gtcgaagtgc aaatgcaggc agccttgcaa gatgggcaac ttgatgtgaa cgagcaacaa
      421 attctgttta cgattgccga aacaatggga atgtctcgct tccaatttga gcagatgatc
      481 gcaatgatta tggcagctca acaattccgc aatggtggct attatcagca aaatgggagt
      541 tatcatcagc aatctcaagg tggctatcag ggcagttatc agcagcctgc acaaccaagc
      601 attgaggcag cctataccgt attaggcgta accgcacaag acgatcaaaa tacagtgaaa
      661 cgggcttatc gtaagttaat gaacgaacat cacccggata aattagccgc taaaggtttg
      721 cctgatgaga tgatggagtt ggcaaaagag aaagctcaac aaattcaagc ggcgtacgat
      781 ttaatttgta aagtgaaaga gtggaaataa tggattcacg ctcgcatatc atccctctat
      841 ttttagcgat tgttattacc gtgattgcgg tttggtcagg gttaaatccg acagaccgcg
      901 cagtttggta tgcggaagtg ataccgattt ttatcgtttt tgccctttta attatcactt
      961 accctaaatt tcagtttagt gggttagctt attttttgat gtcgctttgg ctgattatgc
     1021 acttgatcgg ggcaaaatat acctttgcca atgtgccgtt tgagtgggca aatcaatttc
     1081 taaccccgat tttaggtgaa gatcgtaacc attttgatcg agtagcacat tacattatcg
     1141 gtttttacag cttcccgatg gcagaatggt tactgcgtaa acgcaagtgc ggaatgggcg
     1201 tagcattttt cttctcgctg tttttcatta tgtcggtggc ggcagcttac gaaattattg
     1261 aatggcaata tgccgtgatt gaaggtggca atgccggcgt ggaatttcta ggctcgcaag
     1321 gggatatttg ggacgctcaa aaagatatgc ttgcggatac tcttggggca ttaaccgcac
     1381 tgatgattta cctgatcgct cgccccgatt tgcgcattca cgatagacat ttttaacctt
     1441 caagcggttg tttcttgcca attttttgca aaatgttgag aaaaaataac cgcttgtatt
     1501 ttttatttta ccaatgaatc caattcctaa cgttattctt gccccgatgc aaggggtttt
     1561 agaccctttt gtccgcaacc ttttaacttc agtgaatgac tacgatctct gtatttccga
     1621 atttgtgcga gtggtcgatc aacgtttacc gaagaaaacc ttttatcgcc tagcccctga
     1681 gctatatcaa ggcggattaa ccgattccgg cacaccagtg cgggtacagc ttttagggca
     1741 acacccacag tggctggcag aaaatgcagc tcttgccatt gagcttggtt cacacggggt
     1801 agatctcaac tgtggctgcc cctctaaaac ggtaaatggc agcaatggtg gggcttcgtt
     1861 attaaaagat ccggaactga tttatcgtgc aaccaaagcg atgcgtgaag ctgtgccaag
     1921 cgataaaatc gtttccgtaa aagtgcgatt aggttgggat tccgttagcc aatgttttga
     1981 aattgccgat gcggtagaac agggcggagc gaatgaaatt accgttcacg ggcgaaccaa
     2041 agttgatggt tatcgagcag aaagaattaa ttggcaggcg attggcgaaa ttcaacaacg
     2101 gctgtctatt cctgttatcg ccaacggcga aatttgggat tttgaatcgg cgaaaaattg
     2161 ccaaaaaata accgcttgca aggcattaat gatggggcgt ggggcgttaa acacaccgaa
     2221 tttaagcaaa gtggtgaaat ataatgttcc gaaaatggcg tggaatgaag tgctacagct
     2281 attgtttaag tacgttcaga tggaaaatca gtttgatacc ggtttttacc acgttgctcg
     2341 catcaaacaa tggctacgtt atttggataa agaatatcct caagcggtag aattatttga
     2401 tatcttgaaa accgaacacg gctatgatgg tctaaaagca catattgaaa aggcagtgga
     2461 ggaataatga gtaagcaaat ttttaatgaa gaagagctaa aaacaaccga gcattttgaa
     2521 cctaaacaag agtttgatct cgaaaatgcg attattgaag aagaggaaaa agcagttgag
     2581 gcggagctga ttattgaaga aagccttaag ccttcccgct tttggattcg agtactactt
     2641 gccacacttg ccttgtttgg cgtggcggta attgctcaaa gtattcagtg gttgattgat
     2701 acgttccagc aaagagagtg gatctatttt gcgttctcga tagtcttttt tattgtcagc
     2761 ttgttgggcg ttggggcgat tatttccgaa tggcgaaagt tggtgtattt gcgaaatcat
     2821 cagaaaaatc aacaaatgag taaccaatta ttgctggaac agctgccttc caatgatggc
     2881 gaacaagcgg tcaaattttg cgaaaatatt gcaaatcagc ttaatcactt gccgagcatt
     2941 gctcaagccg agcaactttg gaaaagcgaa ctgaacgagg cttataatgc caaagaggta
     3001 ctatatctgt ttagcgaaaa tgtgctaaag ccgatagata aacaggttaa gcagatgatt
     3061 tcccgcaatg cggcagaaaa tgcggtgatt gtggcggtta gcccgctggc aatagttgat
     3121 gtgttgttaa tggcggcaag aaatatcgct ttagtgaata aaattaccaa agcctatgga
     3181 atggaattgg gttatatcag ccgcttaaaa ctgttcaaaa tggtgctgaa aaatatggtg
     3241 ttcgccggtg caaccgaaat cgctactgat gttggaatgg atttcttctc gcaaaacctt
     3301 acggcgaaat tatcgctccg tgccgcacaa ggtattggtg tgggtttact cactgctcgc
     3361 cttggcatta aagcaatgga attttgtcgt cctgttgctt ttcaagcgaa tgagaagcca
     3421 agaatttcag ctattcgtca ggagttgctt acttcggtca aaaatacggt atttgctaaa
     3481 acagaaacga aagaaaaagt gttctctgat tagaaaatgc tatgggcgga caatgttccg
     3541 cccttaatat tatcgtttgt attggcttgt tggcacatta tccaaatggt cggtattatt
     3601 aaattccagc accgttactt tgccattctc tacttctact ttcgtaatgg cagtattgaa
     3661 cacaaagcta tgtttttctt cattgcggaa atcttgccac gcaatgcctt ttaacacagc
     3721 cgtgagtaag gttaaggtca taccatgtga aacaatcagc actttgccgt cattttggtg
     3781 tagctcggcg atttgattga acgcctgagt cacccgttga taaagttgct caaatcgctc
     3841 gccaccgttt gcttttgcca gatattctgc cggtgtacgt ttcatcaccc aatattcatc
     3901 attttcttgc aaatcgacag atttcattcc ttcccagctg ccaaaatcaa attcattcaa
     3961 gcctttatga tgaaaatgcg gaatattact gcgttcattt tctgctaaaa tgtagtttgc
     4021 ggtatcttga gcccgtttct gcatactgga ataggcggcg gtaaacggaa tatgttttaa
     4081 cgcaatgccg gttttcttcg ctccgataat cccttcttct actaaagcgg aatcgcccga
     4141 gccttgtaat ctgccttcca aattccacac ggttctgccg tgtcgtacta aataaatggt
     4201 aacagccatt gtaatttcct tgctaaaata gtcaaaaatt ggaaggatta tagcaaaatg
     4261 accccaacat tgcctatttg ttatcaacat cgtgatttta ttattatcaa taagccggaa
     4321 ggcttgagtg tgcataaaga tgaggctgaa atcggcttaa cggaattagt ggctcgccaa
     4381 ctgaatgtaa agcaggtttg gctggttcac cgtctggata aaatcacctc aggtttactg
     4441 attttagcgt tgaataaaga ggcggcagca aggttttacc atttatttga acagcataaa
     4501 atctacaaaa cctattgggc attaagcgat aaaaagccga agaaaaagca gggtagaatt
     4561 gttggcgata tgcaaaaaag cagaaatgga gcgtggaaat tatgccacag taaggaaaac
     4621 ccggcagtaa cgcaatttat ttctcattct gttgaagact cgctcaggca ttttattttg
     4681 caacccaaaa caggcaaaac tcaccaatta agagtggcaa tgaaaagttt gggcagcccg
     4741 attttaggtg ataaactcta ttcaggtagc caagccgacc gtgtttattt gcacgcttat
     4801 cagcttgatt ttgaatacga aaatgagaaa atctcagtgc aagccttgcc gacaagcggt
     4861 catttttggc aaaaaatttg caaatccaat cacatcaaac acggagaata gtatgaaact
     4921 ttggtactca accaccagcc ctttcgcccg caaagccctt gcggtgatta aacaccacca
     4981 attagacgat aaagtcgaaa tgttgcgggt gacaaaagca atggatccaa attcgccaca
     5041 taatcaagat aacccgttag gacgtattcc tgccctacaa cgcaactgcg gacgttggct
     5101 tttcggtagt ttgttaatta gcgaatatct tgaccaaaaa ggcaataaca ctccactgtt
     5161 acctaaagag ggtaaacctc gctgggcagt gttggcatta cataatttgg cggacggcat
     5221 tatggaaaat acgatgccaa ctttattaca agaacgtatg gctcgccccg agaatgaatg
     5281 gtgggtcagc cgccacgagc aattaatgga acgcaatatc cgttcgttca gacaattaga
     5341 agaggcattg caagagtttg gcacagagct gaatttaggc acattaaccg cagtagcatt
     5401 aattgactgg tgggcattcc gcttagataa gataggctac gatcttgcaa aaaattaccc
     5461 aaatttaacc gcttgggcag aggaaatgaa taataaatac gccattctgg cagagactaa
     5521 gccgagcatg tagagatttg acatagaaaa taaaggcatt gagttataac tcaatgcctt
     5581 tatttatgag gaaattgttg ttcaatttct tgcaatacag ataaaggatc taaatctaac
     5641 cctttgcaat aagcgataaa ttcaaaaata tctaatctgc gatctcctgt ttctactttt
     5701 ccgataaatg agtaaagaac gcccattttt tctcctaatg tgcgttgtgt tagccctagt
     5761 tccaaacggc gttttaagaa tagttctctt aaccaaatat gctcgctgga atgtattgat
     5821 aatctaagat ttttcattgc acctatatta ggtacaaata atgttgaacc ttttttaggt
     5881 tcattatata atta
//
LOCUS       DANLXS010000153         5671 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.154, whole genome shotgun sequence.
ACCESSION   DANLXS010000153 DANLXS010000000
VERSION     DANLXS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.154"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            100..1218
                     /gene="malK"
                     /locus_tag="PXD71_002119"
     CDS             100..1218
                     /gene="malK"
                     /locus_tag="PXD71_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDL6292197.1"
                     /translation="MANVSLRNVGKSYGDVHISKDINLEINEGEFVVFVGPSGCGKST
                     LLRMIAGLEDITTGELYIGGKLMNEVEPSKRGIGMVFQSYALYPHLDVAENMSFGLKL
                     AGVSKAERDQRVNQVAEILQLAHLLDRKPKALSGGQRQRVAIGRTLVSQPEVFLLDEP
                     LSNLDAALRVQMRVEISKLHKKLNRTMIYVTHDQVEAMTLADKIVVLNAGGIAQVGKP
                     LELYHYPANRFVAGFIGSPKMNFLPVKVIAVEPERVKIELPDANHHNFWIPVSGEGVN
                     VGDNLSLGIRPEHLVPSDQTQVTLHGIVQVVELLGNETQIHLEIPEIKQPTLIYRQND
                     VVLVNEGDDLNIGILPERCHLFKEDGTACKRLFVEKGV"
     gene            1316..2605
                     /locus_tag="PXD71_002120"
     CDS             1316..2605
                     /locus_tag="PXD71_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin"
                     /protein_id="HDL6292198.1"
                     /translation="MKKTVLAVAISGAMFAASASAVDFHGYARSGIGWTSGGGEQSAF
                     TVNGGGSKYRLGNESDTYAELKLGQELFKSGEKSIYFDTNLAYGGTLHNNDWTPTSPA
                     LRELNVQFKNFADSLPGATLWAGKRFYQRHDVHMNDFYYWDISGPGAGVENIDLGFGK
                     LSLAVTRDTERGGANTFGYTTVYKLDPSTGKIKLDKQRNKADVYNDIFDVRLAGIELW
                     KDGSLELGFDYGNAHAKKGAALEKGATKNGYMVTAEYTQGNFFGGFNKFTAQYATDSM
                     TSWNNGHAQGSKADNKGNMLRLINQGVVQASDKVEVMYALIYEKTKLDNKQGKTWYSA
                     GIRPMYKWNDTMSTLLEVGYDRIKDQATGKKNDLVKYTVAQQWQAGNSIWARPAIRVF
                     GTYARWNDKFNTKARTDAGYKAKDGEFITGVQFEAWW"
     gene            2686..3582
                     /gene="malM"
                     /locus_tag="PXD71_002121"
     CDS             2686..3582
                     /gene="malM"
                     /locus_tag="PXD71_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDL6292199.1"
                     /translation="MKKTLLSTAIFFAHLLVVSPSIANGSAGKSIHINSAELAQIQWQ
                     DVPFSQTVKTDLSEQHKQAFTTAFAGIASPVAAYRIPANQGTLEIEIESPVMDKNVFV
                     PTAVVLDSQFNVASTYPSSEFKLLEERGLKGSRLGAELNLTPAMNQDYIYLLIYTTQQ
                     DLAKTTMMPHPAKTYAKATGKQPPAINDIEVAHSLKGQIHINVTGSNGTKFIGLPTEI
                     FSSNKAATPAAKTAIPLPIKENPVAVKTAVDKDTEAYFNQAVMKAIKAKDINKAMNLV
                     NEAEKLGLTSPRQIFLKNVSSN"
     gene            complement(3742..5610)
                     /locus_tag="PXD71_002122"
     CDS             complement(3742..5610)
                     /locus_tag="PXD71_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL6292200.1"
                     /translation="MSGLVSPELIKTVALLATSVTIVPLFKRLGLGSVLGYLVAGCLI
                     GPSGIGLFQDPAAVVHMAELGVVMFLFIIGLEMHPELLWAMRKAIFGRGFLQVAICGT
                     LLTLAGIYLLGFSKEVAFIAGMGFTLSSTAIVMQVLEDKGISSTPKGQRIVATLLFED
                     LAIVPLLASVAFLAPNQVDAESSTNWSAIGLSLVAVLILVASGKWLMNPIFRVISKAK
                     VREMMTAAALLVVLGSALLMEISGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGLL
                     LGLFFMGVGMSLDLSLVLNNWLWLLVIVAVYVIGKGLGIFIAASITRLTLNESIVRTT
                     IMAHGGEFAFVLFSAAATAGILTPDTHATLTAAVIVSMLLSPLLILIKQGVRKKKAKN
                     ESTNMTGVELPEDLEANVLVIGFGRFNQIVCQALLAKGLSVSVIDSNTDHIRAAARFG
                     FKLYYGDGARLDVLRASGIANANCVIVGVGNPERTEKIVELIKSEYPLVPVFARTYDR
                     QSAVDLVKKHHVDYHIRETFESALALSQAAIERLGSSEEEAKQIISYVRRLDAERFNE
                     ELLHGFSAEVIRKYWVPTPWVNPQHEGSALNEETADILEELGETVEVIKVDITDKN"
BASE COUNT         1810 a         1117 c         1185 g         1559 t
ORIGIN      
        1 ctaaaaaaaa gtattttagg atgatttctt ctttagataa aaaggcgact attcagccta
       61 aataaaacca acaagcaaaa gcagaaaaga ggtttagtta tggcgaatgt atcattacgt
      121 aacgtgggaa aatcgtatgg tgatgttcat atctctaagg atattaattt agaaattaac
      181 gaaggagaat tcgttgtttt cgttggaccg tcaggttgtg gcaaatctac gctattacga
      241 atgatagcag gtttggaaga tattacgaca ggagaactct atatcggtgg aaaactgatg
      301 aatgaagtag agccttcaaa acgtggtatc ggtatggtgt tccaatccta tgctctctat
      361 ccacatttag acgttgccga aaatatgtct tttggcttaa agctggcagg tgtaagtaaa
      421 gcagagcggg atcaacgagt taaccaagtt gccgaaatct tacagcttgc ccatttatta
      481 gatcgaaaac ctaaagcact ttccggcgga caacgccaac gggtggctat tggtagaaca
      541 ctggtttccc aaccggaagt atttttactc gatgaaccac tttccaacct tgatgccgca
      601 ttgcgtgtac aaatgcgggt agaaatttcc aaattacata aaaaactaaa ccgcacaatg
      661 atttatgtta cgcacgatca agttgaagca atgacattag cggacaaaat cgtggtatta
      721 aatgccggcg gtattgctca agtaggtaaa ccgttagaac tttatcatta cccggcaaat
      781 cgctttgtag cagggtttat cggctcgcct aaaatgaact ttttaccggt aaaagtgatt
      841 gcggtagagc cggaacgggt aaaaattgag ttacctgatg cgaatcatca taatttttgg
      901 attccggttt ccggcgaagg ggtgaatgtg ggagataact tatctttagg tatccgcccg
      961 gaacatttag ttccttcgga tcaaacccaa gtgaccttac acggtattgt gcaagttgtg
     1021 gaattgctcg gaaatgaaac acaaatccac cttgaaatcc ctgaaatcaa acaaccaacc
     1081 ttaatttatc gtcaaaacga tgtggtatta gttaatgaag gcgacgatct gaatatcggt
     1141 atattgccgg aacgctgcca tttatttaag gaagacggta cagcttgcaa acgtttgttt
     1201 gttgaaaaag gggtataaca atttcagttt attactgatt ttagagggta ttttcaatct
     1261 tctaatcaat taataattaa cctaagtaaa atcaaatctt aagagaggtt ctattatgaa
     1321 aaaaacagtc ctagcagttg caatcagtgg tgcaatgttc gcagccagtg catcagcggt
     1381 tgatttccac ggatatgcac gttcaggtat cggttggaca tcgggggggg gggagcaatc
     1441 cgcattcacg gtaaatggtg gtggttcaaa ataccgttta ggtaacgaat ctgataccta
     1501 tgcagaattg aaattaggtc aggaattatt caaaagtggt gagaaatcta tctactttga
     1561 tacaaacctt gcttacggcg gtacgcttca caataatgat tggacaccaa caagtccagc
     1621 actacgtgaa ttaaatgtac agttcaaaaa ctttgcagat agtttaccag gtgccacctt
     1681 atgggctggt aaacgttttt accaacgcca tgatgtacat atgaatgact tctattactg
     1741 ggatatttca ggtccgggtg caggggttga aaatattgat ttaggcttcg gaaaactctc
     1801 tttagcggtt actcgtgata cagagcgtgg aggggcgaat acttttggat atactacagt
     1861 ttataaatta gatcctagca caggtaaaat taaattagat aagcagcgta ataaagccga
     1921 tgtttacaac gatatttttg atgttcggtt agccggtatt gagctttgga aagatggttc
     1981 gttagagtta ggctttgatt acggtaatgc acatgctaag aaaggtgcag cccttgaaaa
     2041 aggagcaacc aaaaacggtt atatggtaac agcggaatac actcaaggta acttctttgg
     2101 tggtttcaat aaatttactg cacaatatgc aaccgactct atgacttctt ggaataatgg
     2161 gcacgcacag ggttcgaaag cagataataa aggtaatatg cttcgtttaa tcaaccaagg
     2221 ggtagtgcaa gcgagtgata aagttgaagt aatgtatgcc ttaatctacg aaaaaacaaa
     2281 actagataat aaacaaggta aaacttggta ctcagcaggt attcgcccaa tgtacaaatg
     2341 gaatgataca atgagtacat tattagaagt tggttatgat cgcattaaag atcaagcaac
     2401 aggcaagaaa aatgacttgg taaaatacac cgttgcacaa caatggcagg ctggtaatag
     2461 catttgggct cgccctgcaa tccgtgtgtt tggtacttat gcacgttgga atgataaatt
     2521 caacacgaaa gctcgtaccg atgccggcta taaagccaaa gacggcgagt ttatcaccgg
     2581 tgttcaattt gaagcttggt ggtaatcatc aaacctttgt gtgggtagga ttacctaccc
     2641 actatttcag cactttatcc tatttcacga tttttagggg ttaatatgaa aaaaacgctt
     2701 ctttcgacag caatattctt cgcccatctt ttggtggtgt ctccgagtat agcaaatggc
     2761 tcggcaggta agtctattca tattaactcg gctgaattag ctcaaattca atggcaagat
     2821 gtgccatttt cacaaaccgt aaaaacagat ctaagcgagc aacacaaaca agcatttacc
     2881 acagcgtttg ccggtattgc cagcccggtt gcagcttatc gtattccggc aaatcaaggc
     2941 acattggaaa ttgaaatcga aagcccggtg atggacaaaa atgtttttgt acccacggca
     3001 gtcgtgttgg atagtcaatt taatgtggca tcaacttacc cctcttctga gtttaaatta
     3061 ttggaagagc gaggactcaa aggtagccgt ttaggggcag aacttaattt aacgccggca
     3121 atgaaccaag attatattta cttgctcatt tataccacgc agcaagattt agccaaaacc
     3181 acgatgatgc cgcatccggc aaaaacctat gcaaaagcaa ccggtaaaca accaccagca
     3241 attaacgata ttgaagtagc acacagttta aaagggcaaa ttcacatcaa cgttaccggc
     3301 tcgaacggca ctaaatttat tggattaccg actgaaattt tcagctcaaa caaagcagca
     3361 acgccggcag ctaaaacggc tataccgttg ccgataaaag aaaatccagt tgcagtaaaa
     3421 acagcagtag ataaagatac ggaagcctat tttaatcagg cggtaatgaa agcgattaaa
     3481 gcaaaagata ttaataaagc gatgaatttg gttaacgagg cagaaaaatt agggctgact
     3541 tctccacgcc aaattttctt aaaaaatgta agttcaaatt gatttattgt ttttttcata
     3601 gatattctcc ttgtcatcgt gcgatggttt attaccatta acaccctatc agattatgcc
     3661 gatagggtgc ttttttacga gtgattatgc gatatgtata ggaaggctta tacttgaata
     3721 aggattcaaa gtataaattc tttaattttt atcagtgata tccactttta taacttctac
     3781 tgtctcacca agctcttcta aaatatcagc cgtttcttca ttaagtgcac taccttcgtg
     3841 ttgtggattt acccaaggtg ttggtaccca atatttgcgg ataacttctg cggagaaacc
     3901 gtgtaataat tcctcattaa agcgttctgc atctaatcgg cgaacatagc tgataatttg
     3961 ttttgcctct tcctcgctag aacctaagcg ttcaatggcg gcttggctta atgcgagggc
     4021 tgattcaaag gtttctcgaa tgtgataatc aacgtggtgc tttttgacta aatcaaccgc
     4081 actttgtcta tcataggttc tcgcaaatac aggtactaag gggtattcag atttaatcag
     4141 ctctacgatt ttctccgttc tttccggatt gcctacgcca acaattacgc agtttgcatt
     4201 ggcgatgcca gatgccctaa gtacatctag tctcgctcca tcaccatagt aaagtttaaa
     4261 accaaaacga gcagcagcac gaatatgatc agtatttgaa tcaataaccg atacacttag
     4321 tcctttggct aataaggctt ggcagactat ttggttaaag cgtccaaaac cgatgactaa
     4381 tacattggct tctaaatctt ccggtaattc aacccctgtc atattagtgg attcattttt
     4441 cgcttttttc tttctcactc cttgcttgat taaaatgaga agtggggaaa gcaacattga
     4501 gacaattacc gcagcggtta aggtagcgtg agtatctggg gttaaaatcc cagcggttgc
     4561 ggcggctgag aatagcacaa aggcaaattc accaccgtga gccataatgg tagttcgcac
     4621 aatagattca ttcagtgtta atcgggtgat agaggcagca atgaagattc caaggccttt
     4681 accgataaca taaactgcta caatcaccaa taaccatagc caattattga ggacgaggct
     4741 gaggtctaat gacattccta cgcccataaa gaataagcct aaaagtaaac cacggaaggg
     4801 ttcaatatca gcttctagtt gatggcggaa agaagactcc gaaagcatta ctccggcaag
     4861 gaatgccccc attgccattg aaagcccact gatttccatt aaaagagcgg agcctaatac
     4921 cacaagtaag gcagcggcag tcatcatttc tcgtactttg gctttagaaa taacacggaa
     4981 aatagggttc atcagccatt ttccactcgc aaccaaaata agaacggcaa ctaggctaag
     5041 accaatagca gaccaattag tactggattc agcatctact tggttaggag caagaaaggc
     5101 aaccgaggct aataatggca caatcgctaa atcttcaaat agtaaagttg caacaatacg
     5161 ttgccctttt ggcgtggaac ttatcccttt atcttccagc acttgcatta caatggcagt
     5221 agaagatagc gtaaaaccca taccggcaat aaatgccact tctttggaaa aacctaataa
     5281 gtaaatgccg gctaaggtta ataaagtacc gcaaatcgca acttgtaaga aacctcgccc
     5341 gaagatggct ttacgcatcg cccaaagcag ttccgggtgc atttccaaac caataataaa
     5401 caaaaacata accacgccaa gttctgccat atgcacaaca gcggcagggt cttggaacaa
     5461 tccaatgccc gatggcccga ttaaacatcc tgcgactaaa taacccaata cgcttcctaa
     5521 accaaggcgt ttaaatagcg gtacaatagt gacgctggtt gcaagtaatg cgactgtttt
     5581 aattaattcc ggactaacta atcctgacat agaattccca cctgtataat ataaataaga
     5641 ttaatgatgt ttttgtgcta aataatctaa a
//
LOCUS       DANLXS010000154         5665 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.155, whole genome shotgun sequence.
ACCESSION   DANLXS010000154 DANLXS010000000
VERSION     DANLXS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.155"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            429..1433
                     /locus_tag="PXD71_002123"
     CDS             429..1433
                     /locus_tag="PXD71_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL6292201.1"
                     /translation="MPGDANKIISNGTSAGGALSTLLGASADHYDYEPYLKEAGALNA
                     SDKIFAVSAYCPITNLENADMAYEWQFNGVNEYSRIDMSRLNAAEFNDRSKPKPKPKI
                     EGSLNEAEIKVSNELAERFPTYLNSLHLVDEKGNPLTLDPKGNGSFKDYLSEVVKTAA
                     NKAYRGLVQDSEEQKAFQQISWLSFEKGKVSSVDWFGYVFSDKRMKSPPAFDALNGSS
                     GENNLFGTDTENNRHFTLYSAERSANKDLNLADPQIVKRMNPMHYLDNPNAAEHWRIR
                     VGTADRDTSLAISAILAIKLQMAGKNVNYETPWNVPHSGDYDVNELFLWADELVKGKK
                     "
     gene            1488..3338
                     /locus_tag="PXD71_002124"
     CDS             1488..3338
                     /locus_tag="PXD71_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL6292202.1"
                     /translation="MQVKKFSQKYINWVLKLGRLKASLLGFFMVALSAILIQSSLTYL
                     FIGRVVVVDILRSIAFGLISAPFVLYFFTLLVEKLEISRLKLEKAVRDLEELREQDAY
                     LNAKLEKNSRDKSILMATISHELRTPLNGIIGLSQILLDDKTTEKQQEYLKTINMSAT
                     ALAHIFGDIIDLEKIDSSRIELVRKEVEFEQLIHDIANFGHFMAGNKQINFEVKYPPD
                     LPKYIYIDHARLSQILWNLISNAVKFTPASGRILLEIYQLDKEQFSFAVTDSGIGIPE
                     KDQRKIFDMFYQAENSKEKKAQGSGIGLSVSKRIAKLMGGDLVVASQENQGSTFTLTV
                     QAESTETKPIFAIQQHSLKVLLVEDIEMNVVVARAMLEKIGCVVDVAMSGEETFQHFA
                     QNSYDLILLDIQLPDTTGFEIAQQLRLQYENEEIDYLPLLVALTANVIQSRQEYQAQG
                     MDDVLHKPLSLDELMRCLNQHFGDDFLQKNEEKQPLVERGSENLPYSITILNELIGVM
                     GKENVLANMDLFADLMPTYLANLQRYLSDWQTTNSVESRKLTADEAHKIKGALASVGL
                     HQLQQTAQLAQSDNGEQWEQNIAHWVEMICSKWQMDLQLAKEYVRSKGDI"
     gene            complement(3434..3988)
                     /locus_tag="PXD71_002125"
     CDS             complement(3434..3988)
                     /locus_tag="PXD71_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL6292203.1"
                     /translation="MKSQIYYDYYPSPIGQLLLLATEEGLIYIELGQEQQTTSLDNFL
                     SAQAASGQILEIFCKTKEILDRYFAGEPLDFQQLDFLAPQGTPFQQSVWKILREIPYG
                     QTTTYGEIATQLGKPSAMRAVGGAVGRNPISILVPCHRVLGKNQALTGFGGGLPNKRF
                     LLQLEDITYKDKGIEFVKPKTKKW"
     gene            complement(4055..5536)
                     /locus_tag="PXD71_002126"
     CDS             complement(4055..5536)
                     /locus_tag="PXD71_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HDL6292204.1"
                     /translation="MKMKNFSKTLIASLVAFSSAGASAAAFQLAEISTSGLGRAYAGN
                     AAVADNASVVATNPALMTLFKQTEISAGGIFVDADVNIDGKLNATGADASHKNVIPTA
                     IVPNAYIVSPINERLSIGGGVNVNYGLISEFDPNYSAGFLGGRTSLSAVNVNFSAAYD
                     LGYGFVLGAGLNAVHAKAELGRFLGAGAARFAPAKQFLEDKKVQVQSAIQGLKALEAG
                     IAAGNTALEAQKTQVLAQVNGLLKSQNLNQTITNSAQLERLVNGIQNTNASSTIHKLK
                     GDKWALGWNVGLVYNINERNRLGFTYHSEIDLKFKGKYSNTVPVVIPKMVDTITGGAE
                     IDGTLRLRLPAFWEVSGYHKLTDKLAAQYSYKRTDWSKFQSLEAYDMQGNRLLHKTEN
                     FNDSQRIALGLSYDVNEMLTLRTGVAFDESASVTHPSISIPDTDRAWYSVGATVRFTP
                     NLSADVGYSHLRGSNLTFSEDGLATFKSKARANLYGLNVNYKF"
BASE COUNT         1791 a         1018 c         1197 g         1659 t
ORIGIN      
        1 tatattgaca gttataaata tcatcttgtc cataaatgat caatgatcat gcaaaaatga
       61 accaggcaga cgcgatgaga tgatcgactt catcttttga taaactcttt gcgatttaaa
      121 cagaaaatat atttagaatg taaacaaaca tgagtcgatt ttatgttcaa agagctgtaa
      181 taagacggaa taatgtcaga ccaagataga tactttcaag taaaatcaag cataaaaatg
      241 attttcagac tttacaaatc tgaaagaatg tgtaagatca ggattgtccg aaattcggat
      301 ggaaaatccc agtggatgta ttggctggat acatcataat aactatgtat ttgtgaatgt
      361 tatttgaatg attctacgta gttgattcaa aatcagcagt gcggtatctg catgctaatg
      421 atgaaaaaat gccgggcgat gcaaataaaa ttatctctaa cggcaccagt gccggaggag
      481 cattatctac tttgttagga gcttcagctg atcattacga ctatgagcct tatttgaaag
      541 aggcgggggc attgaatgcc agtgacaaaa tttttgcagt ttctgcctat tgcccaatta
      601 ccaaccttga aaatgcggat atggcgtatg aatggcaatt taatggtgtc aatgaatata
      661 gccgaatcga tatgagtcga ttaaatgcag cagagtttaa cgaccgctca aaaccaaaac
      721 caaagccgaa aattgaaggc tcgttaaatg aggcggaaat taaagtttct aatgaattag
      781 cagaacgctt tccgacttat ttgaacagtt tgcatttggt tgatgagaaa ggcaatcctt
      841 taacacttga tccaaaaggc aacggctcgt ttaaagatta tttatctgaa gtggtaaaaa
      901 cggctgcaaa taaagcctat agaggtttag ttcaagatag cgaagagcaa aaagcctttc
      961 aacaaattag ttggctttca tttgagaaag gcaaagtcag cagtgtggac tggtttggtt
     1021 atgtgttttc agataaacgg atgaaatcac cacctgcatt tgatgcgtta aatggaagtt
     1081 cgggcgagaa taatttattt ggcacagaca cggaaaataa ccgccatttt acgctttata
     1141 gtgcggaacg ttctgccaat aaagatctca atttagccga tccgcaaatc gtgaaacgga
     1201 tgaacccgat gcactatttg gataacccaa atgctgcgga acattggcgg attagggtgg
     1261 gaacggcaga tagagatacc tcgctggcaa tttctgcaat cttagcaata aaactgcaaa
     1321 tggcaggcaa aaatgttaat tatgaaaccc cgtggaatgt accccattca ggtgattatg
     1381 atgtaaatga attattccta tgggctgatg agctagtcaa aggcaaaaag taaaaaatag
     1441 agtaaaataa agcagatctg atggtctgct ttttgttttg aggtcgtatg caagttaaaa
     1501 aatttagcca aaaatatatt aattgggtat taaagctagg gcggttaaaa gcttctcttt
     1561 taggcttttt tatggtcgct ctttcggcaa ttttaattca aagtagctta acctatcttt
     1621 ttattgggcg agtagtcgtg gtggatattt tacgctccat cgcttttggt ttaatttctg
     1681 cgccttttgt gctttatttc ttcacgttat tggtggaaaa acttgaaata tctcgcttga
     1741 aattagaaaa agcagtaaga gatttggaag aactccggga acaagatgcc tatcttaacg
     1801 ctaaattaga aaaaaacagc cgtgataaaa gcattttaat ggcgaccatt agtcacgaac
     1861 ttcgtactcc ccttaatgga attatcggct tgagccaaat tttgttagat gataaaacca
     1921 ccgaaaaaca gcaagaatat ctaaaaacta tcaatatgag tgccactgca ttggctcata
     1981 tttttggcga tattattgat ttagaaaaaa ttgatagtag ccgaattgaa ttggtaagaa
     2041 aagaggtgga atttgaacaa ctcattcacg atattgctaa ttttgggcat tttatggcgg
     2101 gcaataagca gattaatttt gaagtgaagt atccgcctga tttgccgaag tatatctata
     2161 tcgaccacgc acgcttgagc caaattttat ggaatttgat cagcaatgcg gtgaaattta
     2221 cccctgcaag cggtcgaatt ttgctggaaa tttaccaatt agataaagaa cagtttagct
     2281 ttgcggtgac ggattcaggc atcggtattc cggaaaaaga tcaacgcaaa atttttgata
     2341 tgttctacca agcggaaaac tctaaagaga aaaaagcgca aggtagcggg attggcttat
     2401 cggtctcgaa acgcattgcg aagctaatgg gcggtgattt ggtggtggca agccaagaaa
     2461 atcaaggctc aacttttacc ctcactgtgc aggcagaaag cacggaaacc aagccgatat
     2521 ttgctattca gcaacattcc cttaaggtgc tattggtaga agacattgaa atgaatgtgg
     2581 tggttgcccg agcaatgttg gagaaaatcg gttgcgtggt tgatgtagca atgagtggtg
     2641 aagaaacgtt ccaacatttt gcacagaaca gctacgatct gattttactt gatattcaac
     2701 tgccggatac caccggtttt gaaattgcac agcaattaag attgcaatat gaaaacgagg
     2761 aaattgacta tttaccgctg ttggttgctt tgacggcaaa tgtgatccaa agccggcagg
     2821 agtaccaagc acaaggtatg gacgatgtgc tacacaaacc gctctcttta gatgaattaa
     2881 tgcgttgtct caaccaacat tttggagatg attttttgca aaaaaatgaa gaaaaacagc
     2941 cgcttgtaga gagaggttct gagaatttac cttatagcat tacaattctt aatgaattga
     3001 ttggggtaat ggggaaagag aatgtgcttg ccaatatgga tttatttgct gatttaatgc
     3061 ccacttattt ggcaaattta cagcgttatt taagtgattg gcaaaccaca aattctgtgg
     3121 aaagccgtaa attaacggcg gacgaggctc ataagattaa gggagcatta gcatcagtcg
     3181 gattacatca gctacaacaa actgctcagc ttgctcaatc cgataacggc gagcagtggg
     3241 agcaaaatat tgcacactgg gtggaaatga tttgttccaa atggcagatg gatttgcaat
     3301 tagctaagga atatgttaga agcaaggggg acatttagta aatttgaaat gttacgatgg
     3361 tgcaagcgtc cacgcttgta ccaattttaa tatctgaagc acaagcgtgg acgcttgtgc
     3421 tatcaaaacg tatttaccat ttcttggttt tcggttttac aaattcaatg cctttatctt
     3481 tataggtaat atcttccagc tgtaatagaa aacgcttatt cggcaagcca ccaccgaatc
     3541 cggttaaggc ttgatttttc cctaataccc tatggcaagg cactaaaatc gaaatcgggt
     3601 ttcgcccaac tgcaccgccg acggctcgca tcgcacttgg cttgcctagt tgggtggcaa
     3661 tttcgccata agtagtagtt tgcccgtaag ggatttcacg caaaattttc caaactgatt
     3721 gctggaatgg agtgccttgc ggggcaagaa aatccaactg ctgaaaatcc aatggctcgc
     3781 cggcaaaata gcggtctaaa atttctttgg ttttgcaaaa aatttctaaa atctgaccac
     3841 ttgcagcttg agcggaaagg aaattatcaa gcgaggtggt ttgttgctct tgccctagct
     3901 caatgtaaat caagccttct tctgtggcta aaagcaataa ttgcccgata gggcttgggt
     3961 agtaatcgta gtaaatttga gatttcatgt ttttatgcca ataaaaaaag aggtgtaatt
     4021 gattttacac ctctttaaac cttaattcat caaattagaa tttgtagttt acgtttaaac
     4081 cgtaaagatt tgcacgagct ttgctcttga atgtggctaa accgtcttca gagaaggtca
     4141 ggttgctacc acgaagatgt gagtaaccta catcagcaga taggtttggc gtgaaacgca
     4201 cggttgcacc tacagaatac caagcacgat cagtgtccgg aattgagatt gatggatgag
     4261 ttacacttgc actttcatca aatgctacac ctgtacgcaa tgttagcatt tcattcacat
     4321 cataagacaa gcctaatgca atacgttgag agtcattaaa gttctcggtt ttgtgcaata
     4381 ggcggttacc ttgcatatca taagcttcta ggctttggaa tttgctccag tctgtgcgtt
     4441 tatagctgta ttgtgcagca agtttatcgg ttagtttgtg gtaacctgac acttcccaga
     4501 atgccggtag gcgtaatctt agtgtaccat caatctctgc accgccggta atggtatcta
     4561 ccatcttagg aataactaca ggaacggtat tagaatattt gcctttaaat ttaagatcaa
     4621 tttcagagtg gtaagtaaaa cctaaacggt ttctttcatt gatattataa accaagccaa
     4681 cattccaacc caatgcccat ttatcgcctt taagtttatg aattgttgaa cttgcattgg
     4741 tattttgaat gccattaacc aaacgttcta attgagcaga attcgtgatg gtttggttta
     4801 aattttgaga ttttaataaa ccgtttactt gagctaatac ttgtgttttt tgagcttcaa
     4861 gagcggtatt acctgctgcg atacctgcct caagagcttt taaaccttga attgcacttt
     4921 gtacttgtac tttcttgtct tccaaaaatt gtttagctgg agcaaaacgt gccgcaccgg
     4981 cacctaagaa acgtcctaat tctgcttttg cgtgtactgc attcaaacct gcccctaata
     5041 caaaaccata acctaaatca taagccgcac taaaattaac attcacagca cttaatgatg
     5101 tacgaccacc taagaaacca gcagaatagt tagggtcaaa ttcagatatc aaaccataat
     5161 ttacatttac gccaccacca atagaaaggc gctcattgat aggtgaaacg atataagcat
     5221 ttggaacaat agcggtagga atgacatttt tatgtgaggc atctgcacct gtagcattga
     5281 gctttccatc aatatttaca tcagcatcta cgaaaatacc accggcagaa atttcggttt
     5341 gtttaaataa cgtcattaac gcagggttgg ttgcaacaac agaagcgtta tcggctacag
     5401 cggcattacc tgcgtaagct ctacctaaac cggatgttga aatttcagct aattggaatg
     5461 cggcagcaga tgcaccggct gagctgaatg ctactaatga agcgattaaa gttttgctaa
     5521 agtttttcat tttcatagga aatctcttaa aaaatatata ttttagaatg aataattcat
     5581 aaaagttata agaagaaagt gcagatttta tgtgtcgaag tactctctat caattaaatt
     5641 tcaactgttc cgatcagtta ggagc
//
LOCUS       DANLXS010000155         5600 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.156, whole genome shotgun sequence.
ACCESSION   DANLXS010000155 DANLXS010000000
VERSION     DANLXS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.156"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            63..494
                     /locus_tag="PXD71_002127"
     CDS             63..494
                     /locus_tag="PXD71_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL6292205.1"
                     /translation="MFLSGFLSATLLPGNSEIIFTTLASQHLITYGSLSSFSLFNLLL
                     VATLGNSLGSITTYAIGLLLPRPQNTTNRYSQWALAKSEKYGVLVLLFSWLPIVGDIF
                     CGIAGWLRFSLWQSILFIVIGKLLRYLFLLATLYSVLPHIL"
     gene            complement(548..1417)
                     /gene="htpX"
                     /locus_tag="PXD71_002128"
     CDS             complement(548..1417)
                     /gene="htpX"
                     /locus_tag="PXD71_002128"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDL6292206.1"
                     /translation="MAKRVILFLLTNLAITFVLGIVLNLIFKVTGIQGQSTAGILVLS
                     LVFGFSGSLISLFMSKSMALRSVGAEVITQPRNEAEQWLFNTVQRQAQQANIPMPDIA
                     IYHSADVNAFATGATKSNSLVAVSTGLLNSMTKDEAEAVVAHEVAHIANGDMVTMTLL
                     QGVLNTFVIFLARIISTMAASSRDENGNSSTNTLVFWVVDIVLQMVFGVLATMIAMWF
                     SRQREFRADAGSAQLVGKEKMIAALQRLQRVHEPQEMEGSLAAFMINGSRGKELFMSH
                     PPLEKRIEALRNL"
     gene            complement(1505..2587)
                     /gene="dinB"
                     /locus_tag="PXD71_002129"
     CDS             complement(1505..2587)
                     /gene="dinB"
                     /locus_tag="PXD71_002129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL6292207.1"
                     /translation="MSIQAKHKRKIIHIDMDCFYAAVEMRENPALVGKPVAVGGNANQ
                     RGVLTTCNYEARKFGLHSAMPTATALKKCPNLILLPVNMALYKSVSAQIHEIFHRYTD
                     VIEALSLDEAYLDVTECERYFGSATWIAQAIRNDIFNELHLTASAGVAPLKFLAKITS
                     DQNKPNGQCVITPDDVQNFVKNLPLAKIPGVGKVTNEKLKQLGFETCGDIQQAEKAVI
                     YRAFGKFGQRLWDFSHGIDERAVEPYRERKSLAVENTLLHDISTLEQAEKIVADLFDK
                     LVFRLQRNWGDTPLTEFKKLGIKLKFDDFSQTTLERTTDGFAYERFGELLQQIFTRRK
                     ERKVRLIGLSVHFPEKKVNKQLNLWE"
     gene            2710..3621
                     /locus_tag="PXD71_002130"
     CDS             2710..3621
                     /locus_tag="PXD71_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HDL6292208.1"
                     /translation="MLYGLAIVLIPLFLGYLITLKNHRHMAIVNRVVSLCLYLILLVM
                     GISLGQIDNILTELPQIGGTAFSLVVILLLSNIIGLVIYDKLQPMKRDKVNQDQLPSR
                     LHLLIDSFKLIGITLLGGIIGYFTKGVVDFPLHASTYVLEVMIFGVGIQLRNSGIPLR
                     EVFFNKRGIYTSIVMVASSLIGGVISAFTLDLSLAKGLTFASAFGWYSLSSVLVNDAW
                     GPIYGSIAFFNDISREILCLFLVPLFMRQFPSTAVGIGGATSLDCMLPIIQKSGGIQI
                     VPLAISFGFIVNLAAPLLLAVFIGLEG"
     gene            3727..5493
                     /gene="aspS"
                     /locus_tag="PXD71_002131"
     CDS             3727..5493
                     /gene="aspS"
                     /locus_tag="PXD71_002131"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL6292209.1"
                     /translation="MMRSHYCGALNRSHLGQTVTLSGWVHRVRNLGRFIFMQIRDREG
                     IVQVFFDEKDEALFKQASALRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNLVV
                     YNNAEVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRFMDDNGFLD
                     IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFMTAEEVRALMEAMIHGLWKETLGVDLGKFPMMTWNEA
                     MTRFGSDKPDLRNPLEMVDVADILKSVDFKVFSGPANDPKGRVVALRVPNGAALTRKN
                     IDDYTQFVGIYGAKGLAWLKVNDMNTGLEGIQSPVAKFLNEEVLKALVKRLNLQKDDI
                     VFFGADNWHVATNAIGALRLKVGNDLGLTDTTAWKPLWVIDFPMFERDDEGNLSAMHH
                     PFTSPKELSPEELVQDPENAVANAYDMVINGYEVGGGSVRIFSQQMQQTVFSILGINE
                     EEQKEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTDNIRDVIAFPKTTAAACLMT
                     EAPSYANPKALEELAIRTIPQE"
BASE COUNT         1574 a         1219 c         1140 g         1667 t
ORIGIN      
        1 tgacagaata tctcacttcc tttttcagtt tattcttttc cgaacaaaat caactcttta
       61 ctatgtttct gagtggattt ctaagcgcta ccctcttacc cggaaattct gaaattatct
      121 tcactacctt agcaagccaa catctcatca cttatggctc tttatcctca ttttcactgt
      181 tcaatttact cttggttgca accttaggta atagtttagg tagcataacc acttatgcga
      241 tagggctatt actccctcgc ccacagaata ctaccaatcg ttattcacaa tgggcattag
      301 ccaaaagtga aaaatatggc gtgttagtct tgctttttag ttggttaccg attgtaggcg
      361 atattttctg tggcattgcc ggctggctac ggtttagcct ttggcaaagc attctcttta
      421 ttgtgattgg gaaattgtta cgttatctgt ttttgctggc aactctctat tcggtgttac
      481 cgcatatttt atagaaaaca aaagcggctg ttttgcaaaa aattttacaa aaacaaccgc
      541 ttgtgcttta taagtttctt aacgcttcaa tacgtttttc taacggaggg tggctcataa
      601 acaactcttt accacggcta ccgttaatca taaaggcagc aagagagcct tccatttctt
      661 gcggttcgtg aacacgttgc aagcgttgta atgccgcaat cattttctct ttaccgacta
      721 attgagcaga gcctgcatct gcacgaaatt cacgttggcg agagaaccac attgcaatca
      781 ttgttgctaa cacaccaaac accatctgta acacaatatc tactacccag aacaccagcg
      841 tattggtgct tgaattgcca ttttcgtcac gtgaacttgc cgccattgtc gaaataatac
      901 gagctaagaa aatgacgaaa gtatttaaca cgccttgtaa taaagtcatt gtaaccatat
      961 cgccattcgc aatgtgcgcc acttcgtgtg ctactaccgc ttcagcttcg tctttagtca
     1021 ttgaattcag caagccggtg ctgactgcaa ctaaagaatt acttttcgtt gctcctgtag
     1081 cgaatgcatt tacatcagcc gaatggtaaa tcgcaatgtc cggcattggg atattcgctt
     1141 gctgagcctg acgttgtaca gtgttgaata accattgctc cgcttcgtta cgaggctgtg
     1201 taatcacctc tgccccaacc gaacgcaatg ccattgattt cgacataaat agcgaaatta
     1261 aagacccact gaaaccgaag actaacgaaa gcaccaaaat accggcagtg ctttgccctt
     1321 gaatgccggt cactttaaaa ataaggttta atacgatccc taaaacaaag gtaatcgcta
     1381 agttagttaa taaaaataga atgacacgtt ttgccataat gttttcaatc tccaaaaata
     1441 tagatgcaat gattgcacga ataagataat gactttagcc ttaatttcaa gtcattattt
     1501 acaattattc ccacaaattt aactgtttat tcactttttt ctcgggaaag tgaacactta
     1561 gaccgattaa tcgcaccttt cgttcctttc ttcgggtaaa aatttgctgt agcaactcgc
     1621 caaatcgctc atacgcaaag ccatcggtgg ttcgttcaag cgtggtttgg ctaaaatcat
     1681 caaacttgag tttaatgcct aactttttaa actcagttaa tggcgtatcg ccccagtttc
     1741 gttgcaaccg aaaaaccagt ttgtcgaata aatctgccac aatcttttcc gcttgctcaa
     1801 gggttgaaat atcgtgcaac agcgtatttt ccaccgccag cgattttctt tcacgataag
     1861 gctctaccgc tcgttcatca ataccgtggc taaaatccca aagccgttgc ccgaatttgc
     1921 caaacgctcg gtaaataacc gctttttctg cttgctgaat atcaccacag gtttcaaagc
     1981 caagctgttt taatttttca ttggttactt tccccacacc cggaattttt gcaagcggta
     2041 gatttttcac aaaattttgc acatcatcag gcgtaatcac acattgtccg ttaggtttgt
     2101 tttgatcgga ggtaattttt gctaaaaatt taagcggagc aactcctgcc gaggcagtta
     2161 aatgcaattc attgaaaata tcattgcgga ttgcttgagc aatccaagtg gcggagccaa
     2221 aatagcgttc gcattcggtt acatctaaat aggcttcatc aagcgacaag gcttcaatca
     2281 catccgtata gcgatgaaaa atttcgtgaa tttgagcgga aaccgattta tacaacgcca
     2341 tattcaccgg caataaaatt aaattcgggc atttcttcaa cgcagttgcc gttggcattg
     2401 cactgtgtaa gccgaatttt cgagcctcgt aattacaggt tgtcagcaca cctcgttgat
     2461 tagcattgcc gcccaccgcc accggcttac cgaccaacgc cgggttttcc cgcatttcga
     2521 ccgctgcata aaaacaatcc atatcaatat ggataatttt tcgcttatgt ttagcttgaa
     2581 tcgacatttg caatattttc cccaaaaacg accgcttgca acacaaataa ctgtatataa
     2641 aaacagtttt attttatgcc cttattgtta gaatttcaag tttaaaaata gcgataaaaa
     2701 ggataaaaaa tgctctacgg tttagccatt gtgctgattc cactttttct cggttactta
     2761 ataaccctca aaaaccaccg ccatatggca atagtaaaca gagtggtcag cctctgcctt
     2821 tacttgattt tattagtgat ggggatttct ctcggacaaa ttgataacat tctcaccgaa
     2881 ctgccacaga ttggcggtac ggcgtttagt ttagtggtga ttttactcct ttcaaacatt
     2941 atcggcttgg tgatttacga caaattacaa ccgatgaaaa gagacaaagt caatcaagat
     3001 caacttccct cccgcctgca tttgttgatt gattccttta aattaatcgg cattacctta
     3061 ttaggcggga tcatcggcta tttcaccaaa ggcgtggtgg atttccctct gcacgccagc
     3121 acctatgtgt tagaagtgat gattttcggg gtggggattc aactgcgaaa cagcggtatt
     3181 cccctgcgtg aagtgttctt taacaagcgg gggatttata cctcgattgt aatggtggca
     3241 agttccctga ttggtggcgt gatttcggca ttcaccttag atttatccct tgctaaaggc
     3301 ttaacctttg cctctgcatt cggttggtat tccctttcaa gcgtattggt aaacgatgct
     3361 tggggcccga tttacggcag tattgccttt tttaacgaca tctcgcgtga aattttatgc
     3421 ctgtttttag taccactgtt tatgcgtcaa tttccgtcca ccgcagtcgg cattggcgga
     3481 gcgacttcat tagattgtat gctccctatt attcaaaaat cgggcggtat tcaaattgtg
     3541 ccgctggcaa tcagcttcgg ttttattgtt aatttagccg ctccattgtt attagccgtt
     3601 tttattgggc tggaaggata aatcaccgct tgtttgcttt tatttatcca tcttcgctga
     3661 attttccgct ataatcctta agttttcccc gaaacttggg atatttattt tcaaaaggat
     3721 aattttatga tgcgttctca ctactgcggt gcattaaacc gctcacacct tggacaaaca
     3781 gttaccttaa gcggttgggt tcatcgtgtt cgtaaccttg gtcgttttat ttttatgcaa
     3841 attcgtgatc gtgaaggcat tgtgcaggtg tttttcgatg agaaagatga agcacttttc
     3901 aagcaagcat cggccttacg ttctgaggcg tgcgtgcaaa ttcaaggtga agtgattgcc
     3961 cgtgatgaat cgcaaatcaa taaagagatg gcaaccggtg aaattgaagt gttagtaaaa
     4021 aatttagtgg tgtataacaa cgctgaagta ttgccgttag acttcaatca aaacaatacc
     4081 gaagaacagc gtttaaaata ccgctatttg gatttacgcc gccctgaaat ggcagaaaag
     4141 ctcaaaaccc gtgctaaaat caccagtttt gtccgccgtt ttatggacga taacggtttc
     4201 ttagacattg aaaccccgat gctgaccaaa gctactcctg aaggtgcgag agattacctc
     4261 gtgccaagcc gtgtgcataa aggcaaattc tatgctctgc cacaatcacc tcagctattt
     4321 aaacagttgc taatgatgtc gggcttcgac cgttattacc aaatcgtgaa atgtttccgt
     4381 gatgaagatt tgcgtgccga ccgccagccg gaatttaccc aaatagacgt ggaaaccagc
     4441 tttatgactg ccgaagaagt gcgtgcatta atggaagcga tgatccacgg cttatggaaa
     4501 gagaccttag gcgttgatct cggtaaattc ccaatgatga cgtggaatga agcaatgacc
     4561 cgtttcggtt cagacaaacc ggatttgcgt aacccactgg aaatggttga tgtggcagat
     4621 attttaaaat cggttgattt caaagtattt agcggtccgg cgaatgatcc gaaagggcgt
     4681 gtagtggcac ttcgtgtgcc aaacggtgca gctctaaccc gtaaaaatat tgatgactac
     4741 acccaatttg tcggcattta tggggcaaaa ggcttggctt ggttaaaagt gaatgatatg
     4801 aacacaggtt tagaaggcat tcaaagcccg gttgcaaaat tcttaaatga agaagtgcta
     4861 aaagccttag ttaaacgctt aaacttgcaa aaagacgaca ttgtattctt cggtgcggat
     4921 aactggcacg ttgcgactaa cgccatcggt gcgttacgct taaaagtggg gaacgattta
     4981 ggcttaaccg ataccacagc gtggaaaccg ttatgggtga ttgatttccc aatgtttgaa
     5041 cgtgatgatg aaggcaactt atcggcaatg caccacccgt tcacttcacc gaaagaatta
     5101 tcgccggaag aattagtgca agatccggaa aatgcggtgg caaacgctta cgatatggta
     5161 attaacggct acgaagtggg cggtggctcg gtacgtattt tcagccagca gatgcaacaa
     5221 accgtgttca gcattttagg cattaacgaa gaagagcaaa aagagaaatt cggtttctta
     5281 ttagatgcgt taaaattcgg tactccgcca cacgcaggct tagccttcgg tttagaccgc
     5341 ttaacgatgt tgattacagg aactgataac attcgtgatg tcatcgcttt cccgaaaacc
     5401 accgctgcag cgtgtttaat gacagaagca ccaagctatg cgaatccgaa agcgttggaa
     5461 gagttagcaa tccgtacaat tccacaagag taatcggtag gggcgaaaca tttttcgccc
     5521 tttttattta acaagcggtc gaaaaagtga aaaactttgc aaaagtaata atcgcaaaat
     5581 tattctctaa ataacataat
//
LOCUS       DANLXS010000156         5577 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.157, whole genome shotgun sequence.
ACCESSION   DANLXS010000156 DANLXS010000000
VERSION     DANLXS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.157"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            350..619
                     /locus_tag="PXD71_002132"
     CDS             350..619
                     /locus_tag="PXD71_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292210.1"
                     /translation="MKNKNFEIAKVVILNEQLGSPKDLELKVVENSLSDEDRYDIKQA
                     VLKAVGNGCSDPEMLASRCCSAIEIINRNGLNTGNGSISTSSNSV"
     gene            complement(597..869)
                     /locus_tag="PXD71_002133"
     CDS             complement(597..869)
                     /locus_tag="PXD71_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas2"
                     /protein_id="HDL6292211.1"
                     /translation="MRNYLISYDLYKAGQNYDGLISYIKSHQVWAAIHKSVWYIKSNK
                     SAEQIRNELLIYLDKNDSVFVTDMNSAAWNNLTEGNGEYIRQNWMR"
     gene            1150..1225
                     /locus_tag="PXD71_002134"
     tRNA            1150..1225
                     /locus_tag="PXD71_002134"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1183..1185,aa:Arg,seq:tct)
     gene            1537..2043
                     /locus_tag="PXD71_002135"
     CDS             1537..2043
                     /locus_tag="PXD71_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292212.1"
                     /translation="MEAKYNMIIELAQQQGLTHIQLTQKHCLHLAASVGKLCSGVVNN
                     AKQKIKTATGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELYGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            2047..2406
                     /locus_tag="PXD71_002136"
     CDS             2047..2406
                     /locus_tag="PXD71_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292213.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKQKQDG
                     ILEVIEYRISQIKELLK"
     gene            2403..2882
                     /locus_tag="PXD71_002137"
     CDS             2403..2882
                     /locus_tag="PXD71_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292214.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKALNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYNFEQIEEDPEQVKPKPSKPKRK
                     PFTPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNALAEMVKRTQEANNPKHA"
     gene            3016..3228
                     /locus_tag="PXD71_002138"
     CDS             3016..3228
                     /locus_tag="PXD71_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292215.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFLLVEEKIIDVLENNGFETVAT
                     ILAGSDGWCNAINAYLAEQNKHRNEF"
     gene            3241..4164
                     /locus_tag="PXD71_002139"
     CDS             3241..4164
                     /locus_tag="PXD71_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL6292216.1"
                     /translation="MTNQNQQVAQKQDKFPIKTLFESPNVKQKLQELLNKNAATFTTS
                     VLQIVNSNSMLRNAEPMSVFNAACMAATLNLALQNGLGFAYIVPYQNRKEKKTEAQFQ
                     LGYKGLIQLAQRSGQFKRLVAVPVYEKQLLAEDPINGYEFDWKQKPAKDEKPIGYYAY
                     FKLINEFTAEIYMTTQEVHDHANRYSQTYRKGYGVWHDNFEAMALKTVMKLLLSKQAP
                     LSVEMQQAVLADQAVVKDVENAEFAYPDNEIQEAEFTELKVSDEVFEKCKQTIINKES
                     TLQDMCDNGYEFNKEQYAELEKLENEQQSNV"
     gene            4157..4819
                     /locus_tag="PXD71_002140"
     CDS             4157..4819
                     /locus_tag="PXD71_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation protein TolB precursor"
                     /protein_id="HDL6292217.1"
                     /translation="MYKFKPRCSMLHQLMSEPRTKADREAGLISDTAKAAVREVAKFD
                     LFGYQSFDGNKYTEKGNQLEEQAIKLSGFTRGLVLKKNTERRENDWISGECDIYVPSR
                     KLIIDTKCSWDIGTHPFFADEAIAKAEKAGYDIQMQGYMWLWDCEEAQIDFVLFPTPI
                     DLIGTYESTEKLIDLVEQIPQQKRITTVTVKRDEAIIAKIKDRVKAASKYYDKLIQEA
                     MN"
     gene            4860..5561
                     /locus_tag="PXD71_002141"
     CDS             4860..5561
                     /locus_tag="PXD71_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDL6292218.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAITTFGTVFAGTLGGYIE
                     KESNLKHLGNAWVYDNAKVYDSAEVYGNAKVYDSAEVYGNAEVWGSAEVYGNAKVWGN
                     AEVWGSAEVYGNAEVWGSAKVWGNAEVWGSAEVYGNAKVYDSAEVWGSAKVRSFAVLS
                     ERKMIFWASNVGTENGTLTVYNGKDGLLVTRGCFTGTVEEFLAKSDEVHDEKTKREYQ
                     LLIEVAKSRILGEAT"
BASE COUNT         1924 a         1152 c         1185 g         1316 t
ORIGIN      
        1 atgcacccat caaaacgagc tacggctcaa tgctctttaa aaatcaaatt caaaaccgct
       61 ttaagcctaa tggtgaaaag taggaagacg aaagtcggag gcagccctta aaacgcccac
      121 tacttcaaag cgttatgccc tttggtgcaa gcaaaggaat acaagcatat acccttatag
      181 cggtttaggt gaaagtctgg gcgtgtacat tgagacacaa gggagtaacc gaaattcaaa
      241 ttgtgcaaac gcagctctaa aattgcgtga cagctcggag agacggcatt tcaaattcca
      301 aagcatattt gagaaagtgt gttttggaat atttaactag aggtaaatta tgaaaaacaa
      361 aaattttgaa attgcgaaag ttgttatttt aaatgaacaa ctaggaagcc ctaaagattt
      421 ggaattaaaa gtagtagaga atagcctatc tgatgaagat agatacgaca tcaaacaggc
      481 ggtacttaag gctgtaggga atggttgttc agatccagaa atgcttgcat cacgatgttg
      541 ttccgcaatc gaaataatta atagaaatgg gctaaacact ggtaatggga gtatatctac
      601 ctcatccaat tctgtctaat gtattcacca tttccttcag tcagattatt ccaagcagca
      661 ctattcatat ccgtaacaaa gacactatcg ttcttatcta aataaatgag cagttcatta
      721 cgaatttgtt ctgcagattt attagatttg atataccaaa ctgatttatg aatagcagcc
      781 caaacttgat ggcttttaat ataactaatc aatccatcgt aattttgccc agctttgtat
      841 aaatcgtaag aaattaaata gtttctcata gaagaatcct tatttgtgct gtgagagatt
      901 taattatatt ccttagtgtt gtgagagaca ataaggaaac ttgacggctc acaagtataa
      961 aagagccacc atctcaaacc gctctcaaca aagagttagc ccgataacct cacttggttt
     1021 tttgcgtatg ttttcccaaa gtgccgttat ccagcgaaac gaatggacta gagagcggtt
     1081 tgagatggtg aaagccatcc tccttagatt gaagtaaacc ctgccgttgg ggttaagcaa
     1141 cggtactttg accgcatagt tcagtggata gagcagctgc cttctaagca gtgggtcgag
     1201 agttcgaatc tctctgcggt cgccatccaa aaccgctttg cgcacatcat cgatgacgag
     1261 cctacgaagt ggaaacctac cgtgtagagc ggttttgaat ggctaataaa acaatgtgag
     1321 gtatcaaatg cttattaagt taaaaagccc tgaacaagca gggcaagaag aaatctggga
     1381 agttagcgat gattggattg ctcctcaaga attaaatccg ccatcagaaa catctcctta
     1441 atataacgcc tgtagagttg ctcaaggtta agtaattact gcggaggggc tttttacacc
     1501 caaaattcag atttttgact aaaaaaggaa cgaaaaatgg aagctaaata caacatgatt
     1561 attgagctgg cacaacaaca aggcttaacc catattcaac ttactcaaaa acactgtctt
     1621 catcttgctg catcagtggg gaaactatgt tctggagtcg ttaataatgc gaaacagaag
     1681 attaaaacgg ctacgggaga aatttttaca ttactcacca tcattaacca tcaaatccag
     1741 ttcgatgaag acttagagct tcacacgtgg aaagctagtg gaacaacccc acccgagcaa
     1801 agagcgatac gtatgttaca tcatacctcg caatttgctt ggtttgctcc ttggttagtg
     1861 gacagcaatg aaaaagaaca aaaccaaaat acaatcagga ttaatttatt taacaaatgc
     1921 tctgggattc tgattgaact atatggcatt cttaaagaat acgagctaac tcttgatgag
     1981 tgcttagatg aattcttgaa aaataagcat tgtttgaatg aatttagtat tcacaaggag
     2041 taaaaaatga cgacgtttag tatttatacc agtgtatggc acgacagctc cacgaaaggc
     2101 tttcgccaca acattaagca taaacgccac gattgctgga gagcggatat ccgcataaag
     2161 caaaaaatca atgatgtgtg ggtgcagatt tcaagaatac gcaaacgctt caagtgccct
     2221 gaacaggcaa aatcctttgt agaacaatgg aaacaaattg attcccctgc atttatgcaa
     2281 caccaaatca accgccaagc attaagagaa gaattatcaa accttgagca acgcaaacag
     2341 aagcaagacg gaatacttga ggtgattgag tatcgtatta gccaaatcaa ggagttactg
     2401 aaatgaaacg caataaagta gaaagctata ctatccgctt attaaaaaat aatcatacaa
     2461 aacgctactt cggagaagtt atattaaacg gcataaaaga aatcagcacg gcagagatga
     2521 tgtctgaaat taacgctatt aaggcattaa ataagcgtat tgaagctctg aataaaacca
     2581 aagccgttaa tttgcctaat tatcctgaaa tcagaggtca tcagcctatt atgtacaatt
     2641 acaattttga acaaattgag gaagatcctg aacaggttaa gcctaaacca tcaaaaccaa
     2701 aacgcaaacc tttcacaccc tacgggctaa acggctactt tgtggatagc aacggcaata
     2761 ttcgcctgca cctagacaga cgagcaagtg ccgaaacaat tacccttgaa actgatttct
     2821 tcaacgctct tgccgaaatg gtaaaacgta cacaagaggc aaacaatcct aaacatgctt
     2881 agacaatgga ttacatacgg cttatacgcc ctgcttttta tggttgccat caccctccta
     2941 atgcaatgaa gagcttatcc ctgaaagcac ctgcaagcct gaaacttgcg aatttaaagt
     3001 aacggagaaa gtgtgatgat aggatacgaa gactacacag atgcttcact tcaaccagaa
     3061 gaagacccta gcgaagaggc tttcctctta gtggaggaga aaattataga tgtgctagaa
     3121 aacaacggct ttgaaaccgt tgctactatc ctagccggtt ctgatggctg gtgtaacgca
     3181 attaatgcct acctggccga acagaacaaa cacagaaatg aattttagag gaataagata
     3241 atgacaaacc aaaatcaaca agtagcacaa aagcaagata aattcccgat taaaacccta
     3301 tttgaaagcc cgaatgtaaa gcaaaaacta caagagcttt taaacaaaaa cgcagccacg
     3361 tttacaacaa gcgtgttgca gattgtgaat agtaactcaa tgttacgcaa tgccgaaccg
     3421 atgagcgtat tcaacgccgc ttgtatggca gcaacgctta atttggcatt acaaaacggc
     3481 ttaggttttg cctacattgt gccgtaccaa aaccgcaaag agaaaaaaac cgaagctcaa
     3541 ttccaacttg gctacaaagg tttaattcaa ctggctcaac gtagcgggca atttaaacgc
     3601 cttgttgccg tgccggtgta tgaaaaacaa ctgcttgccg aagaccccat caacggttac
     3661 gagttcgatt ggaaacaaaa accggctaaa gatgaaaaac cgattggcta ttacgcttat
     3721 ttcaaactga taaatgaatt taccgccgaa atctatatga ccacgcaaga agtccacgac
     3781 cacgccaacc gctacagcca aacctaccgc aaaggctacg gcgtatggca cgacaacttt
     3841 gaggcaatgg ctctcaaaac tgtgatgaag ctcttgcttt ccaaacaagc tccattatca
     3901 gtggaaatgc aacaagcggt cttggcagat caagcggtcg taaaagatgt tgaaaatgca
     3961 gaatttgcct accctgacaa cgaaatccaa gaggcggaat ttaccgaact caaggtcagc
     4021 gatgaggtgt ttgagaaatg caaacaaacc atcatcaaca aagaaagcac cttgcaagat
     4081 atgtgcgata acggctacga gttcaacaaa gagcaatatg cggagttgga gaagttagaa
     4141 aatgaacagc aatcaaatgt ataaattcaa accccgttgc tcaatgctcc accaactaat
     4201 gagcgaacct cgcaccaaag ccgaccgtga agccggactg atttccgaca ccgcaaaagc
     4261 agcggtgcgt gaagtggcta aatttgacct attcggttat caatcctttg acggcaacaa
     4321 atacactgaa aaaggcaatc agctagaaga acaagccatt aagttaagcg gttttactcg
     4381 tggcctggtt ctaaaaaaga acaccgaacg ccgagaaaac gattggattt caggcgagtg
     4441 tgatatttat gtaccaagcc gaaagctgat tatcgataca aaatgcagtt gggacatcgg
     4501 cacgcacccg ttttttgcag acgaagccat cgccaaagcc gaaaaagccg gatatgacat
     4561 tcaaatgcaa ggctatatgt ggctgtggga ttgcgaagag gctcaaatcg attttgtgtt
     4621 attccctact ccaatcgacc ttatcggtac ttacgaaagc acagaaaagc tgattgacct
     4681 tgtggaacaa atcccccaac aaaaacgcat taccaccgtg acggttaagc gtgatgaagc
     4741 catcattgcc aaaatcaaag acagagtgaa agcagccagt aagtattacg acaagcttat
     4801 acaagaagct atgaattaat tcaatcgccc cgaaaggggc tttttaatag gtgacaaata
     4861 tgacaaccca aaagaaatac gaactcctcc aaaacgacac aatcaaccac aacggcagaa
     4921 cactctaccg catcaaagcc ataaccacat ttggaacagt ctttgcaggt acacttggcg
     4981 gctatattga aaaagagagc aacctaaaac atttaggaaa tgcttgggtg tatgacaacg
     5041 ccaaagtgta tgacagcgcc gaagtgtatg gcaacgccaa agtgtatgac agcgccgaag
     5101 tgtatggcaa cgccgaagtg tggggcagcg ccgaagtgta tggcaacgcc aaagtgtggg
     5161 gcaacgccga agtgtggggc agcgccgaag tgtatggcaa cgccgaagtg tggggcagcg
     5221 ccaaagtgtg gggcaacgcc gaagtgtggg gcagcgccga agtgtatggc aacgccaaag
     5281 tgtatgacag cgccgaagtg tggggcagcg ccaaagtaag atcatttgca gttctttctg
     5341 aaagaaaaat gattttctgg gcgagcaatg tcggcacaga gaacggtaca ttaaccgttt
     5401 acaacggaaa agacggactg cttgtaacaa gaggctgttt caccggcaca gtcgaagagt
     5461 ttttagcaaa atccgacgaa gttcacgatg agaaaacgaa gcgggaatat cagctactga
     5521 ttgaggtagc aaaaagcaga attttaggag aagcgactta atcagtctct tttttat
//
LOCUS       DANLXS010000157         5532 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.158, whole genome shotgun sequence.
ACCESSION   DANLXS010000157 DANLXS010000000
VERSION     DANLXS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.158"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            116..1249
                     /gene="carA"
                     /locus_tag="PXD71_002142"
     CDS             116..1249
                     /gene="carA"
                     /locus_tag="PXD71_002142"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL6292219.1"
                     /translation="MFKPAILVLTDGSVFSGKSIGADGHTIGEVVFNTAMTGYQEILT
                     DPSYTKQIVTLTYPHIGNTGINLEDKESPNVCASGLIIRDLPLLHSNFRASSSLSDYL
                     IQQNIVAIADIDTRRLTRILREKGAQAGCILVGDATEENIANALAFANSFGSMAGKDL
                     AQSVTCNELYKWTDGEWQLGKGYVPQQQTEFHVVAYDYGVKHNILRMLAERGCRLTVV
                     PAKTSAEAVLALNPDGIFLSNGPGDPEPCDYAITAIRKLLETQKPIFGICLGHQLLGL
                     AAGGKTKKMVFGHHGANHPVQDLDSQKVLITSQNHGFEVDESSLPSNVRVTHRSLFDG
                     TVQGIELTDRPAFSFQGHPEASPGPNDVAYLFDRFVSEMKKVK"
     gene            1352..4558
                     /gene="carB"
                     /locus_tag="PXD71_002143"
     CDS             1352..4558
                     /gene="carB"
                     /locus_tag="PXD71_002143"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL6292220.1"
                     /translation="MPKRTDINTILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPNMADVTYIEPIEWKTVEKIIEKERPDAILPTMGGQTALNCALDL
                     SKNGVLKKYNVELIGATEDAIDKAEDRGRFKEAMTKIGLNTPKSFVCHSFEEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNRDEFIAICERGFEASPTHELLIEQSVLGWKEYEM
                     EVVRDRADNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAINPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLNE
                     LRNDITGGLIPASFEPTLDYVVTKIPRFAFEKFANADDRLTTQMKSVGEVMAMGRTFQ
                     ESLQKALRGLETGICGFNLLSEEPEKIRRELANPGPNRILYVADAFGAGFSLEEVHHY
                     SKIDPWFLVQIQDLVLEELALEKKQFSDLDANEIRRLKRKGFSDKRIAQLTKVSEKMV
                     RDYRHTYAILPVYKRVDTCAAEFASDTAYLYSTYEEECEAKPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHASLALREAGYETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RVENPWGVIVHYGGQTPLKLANALSDAGVNIIGTSADSIDAAEDRERFQQILNTLGLK
                     QPANRTARNALEAVALANEVGYPLVVRPSYVLGGRAMQIVYNDEELNRYMREAVSVSN
                     DSPILLDHFLNNAIEVDVDCICDGENVLIGGIMQHVEQAGIHSGDSACSLPAYSLSGH
                     VLDEIRKQTNAMAFALGVKGLMNVQFAVQNEVVYVLEVNPRASRTVPFVSKATGRPLA
                     KIAARVMAGETLADLNALEEIIPSKFFVKEAVFPFIKFPGVDTILGPEMRSTGEVMGI
                     GNTFAEAFFKAQLGAGERIPKVGKVFMSVIEEHKPALIYIAKRFQEQGYGLCATFGTA
                     NFLRQHGIAVQTVNKVREGRPHIVDAIKNGEIALVINTVSGEPEVIADSHSIRRTALQ
                     QRVPIYTTLAGADAISYGVKHINEFDVYSVQELEQKAN"
     gene            complement(4633..5424)
                     /locus_tag="PXD71_002144"
                     /pseudo
     CDS             complement(4633..5424)
                     /locus_tag="PXD71_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
BASE COUNT         1586 a         1260 c         1232 g         1454 t
ORIGIN      
        1 gaaagaaata ggcaaaataa ctaattttta cttgaatgca aacgtttgct tgattaaact
       61 tagtcgccta cttctaaggc tacacagata aaatttgatg attaggagta aaacaatgtt
      121 caaaccagca attctggttc tcactgacgg ttctgtcttt tccggcaaat ccattggtgc
      181 tgacggtcac actatcggtg aagtggtatt taacactgcg atgaccggtt atcaagaaat
      241 ccttaccgat ccttcttata ctaaacaaat agtcaccctg acttaccccc acattggcaa
      301 taccggcatt aatttagaag ataaagaatc ccccaatgtt tgtgcctccg gcttaattat
      361 tcgtgatctt cctcttctcc atagcaactt ccgtgcgtcc tcctcacttt cagattatct
      421 cattcaacaa aacattgtcg ctattgccga tattgatacc cgccgtttaa cccgaatttt
      481 acgcgaaaaa ggggcacaag ccggttgtat tttagtaggc gatgccacag aagagaatat
      541 tgctaacgct ttggcatttg ccaacagttt cggttctatg gctgggaaag atttggctca
      601 aagtgtcacc tgcaatgaat tatataaatg gacagatggt gaatggcaat taggtaaagg
      661 ttatgtaccg caacaacaaa ccgagttcca cgtagttgcc tatgattacg gtgtaaaaca
      721 caacattttg cgaatgcttg ccgaacgagg ctgtcgctta accgttgtgc cggcaaaaac
      781 ctctgctgaa gctgtgcttg cgttaaatcc ggacggtatt ttcctttcta acggcccggg
      841 ggacccggag ccgtgcgatt atgccattac cgcaatccga aaattattgg aaacgcaaaa
      901 acccattttc ggcatttgct tagggcatca acttttaggt ttagcggcag gcggtaaaac
      961 caaaaaaatg gtgtttggtc atcacggagc taatcacccg gtacaagatt tagacagcca
     1021 aaaagtgctc atcaccagcc aaaaccacgg ttttgaagta gatgaatcca gcttaccatc
     1081 taatgtgcga gtgactcacc gctcactgtt tgacggcacg gtgcagggga ttgaactcac
     1141 tgatagaccg gcgttttcgt tccaaggtca cccggaagcc agcccggggc cgaatgatgt
     1201 tgcctattta tttgaccgat ttgtcagtga gatgaaaaaa gtgaagtaag gacgtaccta
     1261 tgtgtgtcca tcaagcggtc gaaatttgca aaaaacttac caaaaatgac cgcttgtagc
     1321 ctgaaataaa cgagataaaa gagagcccaa aatgccaaaa agaacagaca taaacactat
     1381 cctaattatc ggtgccggtc cgattgtgat cggtcaagcg tgtgaatttg actactccgg
     1441 agcccaagcg tgcaaagcgt tgcgtgaaga agggtataaa gtggtgttgg tgaactccaa
     1501 ccctgcaacc attatgaccg acccgaatat ggcagatgtg acttacattg agccgattga
     1561 atggaaaacg gttgaaaaaa tcattgaaaa agaacgccct gatgccattt taccgactat
     1621 gggcgggcag acagcgttga actgtgcctt agatctttcg aaaaacggtg tgctgaaaaa
     1681 atataatgta gagctgatcg gagctaccga agacgcgatt gataaagccg aagatcgtgg
     1741 tcgttttaaa gaagcaatga ctaaaatcgg cttgaatacg ccaaaatcat tcgtttgcca
     1801 cagttttgaa gaggccttag cagcacaaga gcaggttggc tttccaaccc tgattcgccc
     1861 ctctttcacg atgggaggct ccggcggtgg tattgcttat aatcgagatg aatttatcgc
     1921 catctgcgaa cgtggttttg aagcctcgcc tactcacgaa ttactgattg aacaatccgt
     1981 actaggctgg aaagagtatg aaatggaagt tgtgcgtgac cgtgccgata actgcattat
     2041 cgtctgctcg attgaaaact tcgacccgat gggtgtacac accggcgact ccattaccgt
     2101 tgctccggca caaacgctaa ccgataaaga gtatcaaatt atgcgtaacg cttcgctggc
     2161 ggtattgcgt gagatcggcg tggacaccgg cggttcaaat gtgcaatttg cgattaaccc
     2221 tgcaaacggc gagatgatcg tcattgaaat gaaccctcgt gtgagccgtt cttctgcact
     2281 cgcatctaaa gccaccggct tcccgattgc caaagttgcg gcgaaattgg ctgtaggcta
     2341 cacactcaac gaattgcgta acgacatcac cggagggctg attccggctt cattcgagcc
     2401 gactttagat tatgttgtga ccaaaattcc acgcttcgcc tttgaaaaat ttgccaatgc
     2461 agacgaccga ttaaccactc aaatgaaatc ggtaggcgaa gtaatggcaa tgggaagaac
     2521 cttccaagag agtttacaaa aagccttgcg aggcttggaa accggcattt gcggctttaa
     2581 cttgctttcc gaagagcctg aaaaaatccg ccgtgaactt gccaaccccg gaccaaaccg
     2641 tattttgtat gttgccgatg cctttggagc aggcttttca ttggaagaag ttcaccacta
     2701 ttctaaaatc gacccgtggt tcttggtgca aattcaagac ttagtacttg aagaattagc
     2761 gttagagaaa aaacagttta gcgatctaga tgctaacgaa atccgccgct taaaacgcaa
     2821 aggcttctca gacaaacgta tcgcacagct taccaaagtc agcgaaaaaa tggtgcggga
     2881 ttaccgccat acttacgcta ttctaccggt gtataaacgg gtagatacct gtgcagccga
     2941 gtttgcctca gatacggctt acctctattc tacttacgag gaagagtgtg aagccaaacc
     3001 gtctgatcgc aaaaaagtga tgattttagg cggcggacca aaccgtatcg ggcaggggat
     3061 tgaatttgac tactgctgcg ttcacgcctc gcttgctctg cgtgaggcag gttatgaaac
     3121 cattatggtg aactgcaacc ctgaaaccgt ttccaccgat ttcgacacct ccgaccgcct
     3181 ctatttcgag ccgctaacat tagaagatgt actggaaatt gtgcgtgtag aaaacccgtg
     3241 gggcgtgatt gtgcattacg gcggtcaaac accactgaaa ttagcgaatg ccttatctga
     3301 tgccggtgta aacattatcg ggacttccgc cgacagcatt gatgccgccg aagaccgtga
     3361 acgtttccaa caaattctaa acactctcgg tttaaaacag ccggcaaatc gcaccgcccg
     3421 taatgcgttg gaagcggtgg cattggcaaa tgaagtaggc tatccgctgg tggttcgccc
     3481 atcttatgtg ctaggtggtc gggcaatgca aattgtgtat aacgatgaag agctaaaccg
     3541 ctatatgcgt gaagcggtat cggtttccaa cgacagcccg attttactcg accacttcct
     3601 caataatgcg attgaagttg atgtggattg catttgtgac ggcgaaaatg tactcattgg
     3661 cggcattatg caacacgttg agcaagccgg tattcacagt ggcgatagtg cctgctcact
     3721 acctgcctat tcgttaagcg gtcatgtttt ggacgaaatt cgcaaacaaa ccaacgcaat
     3781 ggcgtttgct ctcggtgtga aaggcttgat gaacgtacaa tttgcagtac aaaatgaggt
     3841 cgtttatgtg ttggaagtta accctcgagc cagccgtact gtaccgtttg tcagcaaagc
     3901 taccggtcgc ccactggcaa aaatcgctgc tcgcgtgatg gctggtgaaa cccttgccga
     3961 cttaaatgca ttagaggaaa ttatcccaag caaattcttc gttaaagaag cggtattccc
     4021 attcatcaaa ttcccgggtg tggataccat tctcgggcct gaaatgcgtt ctaccggcga
     4081 agtgatgggc ataggtaata cctttgctga agcgttcttc aaagcacaac tgggtgccgg
     4141 tgaacgaatt ccaaaagtcg gcaaagtgtt tatgtcggtt attgaagagc ataaacctgc
     4201 tttaatttac attgccaaac gtttccaaga acaaggctac ggtttatgtg ccaccttcgg
     4261 cactgcaaac ttcctacggc aacacggaat tgcagtccaa acggtaaaca aagttcgtga
     4321 agggcgaccg catattgtcg atgcgatcaa aaacggcgaa attgcgttgg taatcaacac
     4381 cgtcagcggc gagcctgaag tgattgccga cagccactct atccgccgta ccgccttgca
     4441 acaacgtgtg cctatttaca ccacccttgc aggtgctgat gctatctcct acggcgtaaa
     4501 acatatcaat gagtttgatg tgtattcagt gcaggaatta gaacaaaagg ctaactaaaa
     4561 taaagctaaa atgacaagcg gtcagatttt gcaaattttt tacaaattct gaccgcttgt
     4621 gtttgtagag gattatttag taaatttata tgctaaatca ttccagcgta attcattttt
     4681 gagttgtggt agctcggtat tttggttgat atggataaac tcaataccaa aatattcagc
     4741 gaacattcgt agcatatcgg catcaatatc taaactaaat acactatgat gagcaccgcc
     4801 ggctagaatc catgcttgag tacctacata aaaatttggt tgaagcttcc aaaaagcatg
     4861 cccaactggt aaatttggca ttggatttgg tgaggcgata gcgtctaaat ccgcaacaac
     4921 cattctaaaa cggtctccca tatctacaac ggttgcattc actgcttttc ccgccttact
     4981 actgaaaatt aagcgaacag gatctgcctt accgccaata cctagcggtc gaatggctaa
     5041 tgttgatttc tctttggcaa tagacggaca aacttcaagc atatgagccc ccaataccac
     5101 ttggttttgt tcatctaaat cataggtgta gtcttccata aaggaagaac cgtttggtaa
     5161 gccataaccc atcactttca cttcacgcac aagagctgcg gttttccaac ctccttcacc
     5221 accaaatccg tagccttgtt ccattaaacg ctgaacagcc aagcccggta attgtttaat
     5281 cccatttaga ttttggaaat tatcagtaaa agctttaaat ccaccattat ctaagaatgt
     5341 ttttaagccg atttcaatac gagcagaata tgcgagagat tctcttgctg aaccattttc
     5401 ttgcaagcta tcctcaacat tatacaagcg gtcagatttt gcaaaaaaat gaccgcttgt
     5461 tgccatttct aaagtgtgat ctacctctca aatttacaaa attttttata acatattgat
     5521 ttaaaatgtt tt
//
LOCUS       DANLXS010000158         5511 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.159, whole genome shotgun sequence.
ACCESSION   DANLXS010000158 DANLXS010000000
VERSION     DANLXS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.159"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            28..1680
                     /locus_tag="PXD71_002145"
     CDS             28..1680
                     /locus_tag="PXD71_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL6292221.1"
                     /translation="MTIFQVAQNWLAQDPDAETRAELEQLIQAAESDEKAKAELTARF
                     DGRLQFGTAGLRGRLQAGSMGMNRVLVAQAASGLAGFIKGYDKEPSIVIGYDGRKNSD
                     VFARDTAEIMAAAGIKTYLLPRKLPTPVLAYAIQYFDTTAGVMVTASHNPPEDNGYKV
                     YLGKANGGGQIVSPADKEIAALIDKVAAGDIRDLPRSQDFTVLDDEVVNAYIEKTASL
                     AKRPKAEINYVYTAMHGVGYEVLSKTLEKAGLPQPYLVSEQIQPDGSFPTVNFPNPEE
                     KGALDLAIKLAKEKNAEFIIANDPDADRLAVAVPDAQGNWKPLHGNVIGCFLGWYLAK
                     QFHAQGKQGVLACSLVSSPALAEIAKKYGLSSEETLTGFKYIGKVENLLFGFEEALGY
                     LVDPDKVRDKDGISAAIMFLDLVCSLKQEGKTLADYTTEFVQEFGAYVSGQISIRVCD
                     LAEIGKLMTALRNNPPSEIGGFKVAEFIDHTKTTRQNDILVFVLENGSRLIARPSGTE
                     PKIKFYLDARGTDAENAEQVLAQFDESVRSLLRQEEYGKQDC"
     gene            1733..2667
                     /locus_tag="PXD71_002146"
                     /pseudo
     CDS             1733..2667
                     /locus_tag="PXD71_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
     gene            complement(2689..4770)
                     /gene="recG"
                     /locus_tag="PXD71_002147"
     CDS             complement(2689..4770)
                     /gene="recG"
                     /locus_tag="PXD71_002147"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL6292222.1"
                     /translation="MSEQLLDGVPLTALSGVGAAISEKLSRIGINNVQDLLFHLPMRY
                     EDRTRITPIADVRPESFATIEGYVQLTEVQFGKRPILSVTLSDSTSKIMLKFFNFNAG
                     MKNSLATGVRVKAFGEIKRGRFMAEIHHPEYQIIRDNQPLVLAETLTPIYSTTEGLKQ
                     ASLRKLTEQALALLEKVKVAELLPDEYNPHKYSLKEALQLLHRPPPTISAEVLEKGDH
                     PAQKRLIFEELLAHNLAMQQLRLGVKQQYAEALTYQTDIKQRFLDSLPFKPTHAQSRV
                     TADIEQDLAKPHPMMRLVQGDVGSGKTLVAALAALLAIDNGKQVALMAPTEILAEQHA
                     NNFANWLNPFGIEVGWLAGKVKGKARTAQLEAIKNGDVQMIIGTHALFQDNVEFHHLA
                     LVIIDEQHRFGVHQRLTLREKGAKENNYPHQLIMTATPIPRTLAMTVYADLDTSIIDE
                     LPPGRTPITTVVISEDRRAEIVQRVYQVCKQEHRQAYWVCTLIDESEVLEAQAAAAIA
                     EDLQRALPDLRIGLVHGRLKPQEKQAIMAEFKAANLDLLVATTVIEVGVDVPNASLMI
                     IENSERLGLAQLHQLRGRVGRGATASHCVLMYKPPLGKISSKRLQVMRDSQDGFYIAE
                     KDLEIRGTGEILGTKQTGMAEFKVADLMRDRKMIPLVQRYAKQIISENQPLAEALIKR
                     WLDNKAEYTNA"
     gene            complement(4774..5202)
                     /locus_tag="PXD71_002148"
     CDS             complement(4774..5202)
                     /locus_tag="PXD71_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL6292223.1"
                     /translation="MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCIT
                     LSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQTKAFVVNNDIALLASEYHIPNKM
                     DLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA"
     gene            complement(5202..5489)
                     /locus_tag="PXD71_002149"
     CDS             complement(5202..5489)
                     /locus_tag="PXD71_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL6292224.1"
                     /translation="MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDY
                     QQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVEIRKSNKPRPVDFGDE"
BASE COUNT         1439 a         1208 c         1309 g         1555 t
ORIGIN      
        1 agtttttaaa ttacatagga gtaaaaaatg actattttcc aagttgcaca aaattggtta
       61 gcccaagacc cggatgcaga gacccgtgcg gaattagagc agttaattca agcggcagaa
      121 tcggacgaga aagcaaaagc agaattaaca gcccgttttg acggacgttt acagtttggt
      181 acggctggtt tgcgtggtcg cttacaagcg ggttcaatgg gtatgaaccg tgttttggtg
      241 gcacaagctg ccagcggttt agctgggttt attaaaggtt atgataaaga gccgtcaatt
      301 gttattggtt atgacggtcg taaaaattcc gatgtgtttg cccgtgacac cgctgaaatt
      361 atggcagcgg caggcattaa aacgtacctg cttcctcgca aattgccaac accagtgctt
      421 gcctatgcga ttcaatattt tgataccaca gccggtgtga tggtaactgc cagccacaac
      481 ccaccggaag ataacggcta taaagtttat ttaggtaaag ccaacggtgg cggacaaatt
      541 gtttcgccgg cagataaaga aattgctgct ctgattgata aagtagcagc aggcgatatt
      601 cgtgatttac ctcgtagcca agatttcacg gtgttagacg atgaagtggt aaatgcttat
      661 attgagaaaa ccgcctcact agccaaacgg ccaaaagccg aaataaacta cgtttacact
      721 gcaatgcacg gtgtgggcta tgaagtgtta agcaaaacat tagaaaaagc cggcttaccg
      781 caaccgtatt tagttagtga gcagattcag ccggacggtt cgttcccaac ggttaatttc
      841 ccgaacccgg aagagaaagg ggcgttagat ttagcgatta aattggcgaa agagaaaaat
      901 gccgaattta ttatcgccaa cgacccggat gctgaccgtt tagcggtagc cgtgcctgat
      961 gctcaaggca actggaaacc actgcatggc aatgtgatcg gctgtttctt aggctggtat
     1021 ttagccaaac aattccacgc acaaggtaaa caaggcgtgt tggcttgctc gttagtatcc
     1081 tctccggcgt tagctgaaat tgcgaaaaaa tatggtttaa gctcggaaga aaccttaacc
     1141 ggctttaaat atatcggaaa agttgaaaac ctattatttg gctttgaaga agccttaggt
     1201 tatttggttg acccagacaa agtgcgtgat aaagacggca tttccgctgc cattatgttc
     1261 ttagatttag tgtgcagcct caaacaagaa ggtaaaaccc ttgcggatta caccaccgag
     1321 tttgtacaag agttcggggc ttatgtaagc ggtcaaattt cgatcagagt ttgtgattta
     1381 gccgaaatcg gcaaattaat gacggcgtta cgcaacaatc ctccaagcga aattggtggc
     1441 tttaaagtgg ctgagtttat tgaccatacc aaaaccacac gccaaaatga cattttagtg
     1501 tttgtgttgg aaaatggcag ccgtttgatt gctcgccctt caggtactga gccgaaaatt
     1561 aaattctatt tagatgctcg tggcacagac gctgaaaacg cagagcaagt gctggctcag
     1621 tttgacgaaa gtgtgcgttc gctgcttcgc caagaagaat acggcaaaca agattgctaa
     1681 tggcattaat tgagggggag cttaggctct ccctttttta aaggagacaa taatggctca
     1741 aaaaattatt ttagattgtg atccggggca tgacgatgcg attgcgatga tgctcgcttg
     1801 ggggaatccg aatattgatt tgttggcagt gactacggtg gtaggtaatc aaaccttaga
     1861 aaaagtatcg cgtaatgcgt tggcggttgc ggaaattgct catatttgca acgttccttt
     1921 tgccaaaggt tgtgccagac cgctggtgcg tgaagtggaa aatgccccgg atattcacgg
     1981 tgattcggga atggatgggc ctgtactccc tgaatctacc cgacaatttg agaaaaaaca
     2041 cgccgctcaa ttaattattg atttgataat gtcgcacccg gagaaaagca ttaccttagt
     2101 gccgaccggt gggcaataac caatattgct ctagctgccc gccttgagcc ccgtattatt
     2161 gaaagagtga aagaagtggt gctgatgggc ggaggttatc acaccggcaa ctggagtgcg
     2221 gtggcagaat ttaatattaa aattgacccg gaagctgctc atattgtgtt taatgccggt
     2281 tggaaggtga cgatggtcgg cttagactta actcaccaag cacttgcgac ccccgatgtg
     2341 gttgaacgct ttaaggcatt aaacagcaaa tcggggcagt ttgtggtgga attgctggcg
     2401 ttcttcgcca aaatgtataa acaggctcaa ggctttgatg ctccaccggt tcacgatgcc
     2461 tgtgcggtgg cgtatgtgat tgacccaagc ctgattgaag ttcagcaagt gccggtcaat
     2521 attgagctaa caggcacgca tacactgggt atgacggtgg cggatttccg ctacccgcca
     2581 aaacagtgta atacttgggt agcaaaaaaa ttagacaaat cccgcttttg ggatttggta
     2641 attgaatcgg tgaaaaacct gccgtaatct aaataacccg cttgccggtt aagcgttggt
     2701 atattccgcc ttgttgtcta accaacgctt aattaacgcc tctgcaagcg gttgattttc
     2761 gctaataatt tgcttggcgt aacgttgcac cagcggaatc attttgcggt ctcgcattaa
     2821 atccgccacc ttaaattctg ccattccggt ctgttttgtg ccgagaattt cgcctgtgcc
     2881 tcgaatctcc aaatcttttt cggcaatgta gaaaccgtct tggctatcac gcatcacttg
     2941 taaccgcttg ctggaaattt tcccgagcgg cggtttgtac attaagacgc aatgcgaggc
     3001 ggttgcccct cgcccaacac gtccacgcaa ctggtggagt tgagccagcc cgagtcgttc
     3061 ggaattttcg ataatcatta ggctagcatt cggcacgtcc actcccactt caattacggt
     3121 ggtcgcaacc agcaaatcta aattcgctgc cttaaattcc gccataatcg cttgtttttc
     3181 ctgtggtttc agccgtccgt gtactaaccc gatgcgtaaa tccggcaagg ctcgctgtaa
     3241 atcttcggca atcgctgccg cggcttgagc ctccaacact tcgctttcat caatcagggt
     3301 gcagacccaa taggcttggc ggtgttcttg cttacagact tggtacaccc gttggacgat
     3361 ttctgctcgc cgatcttcag aaatcaccac agtcgtgatt ggcgttctac ccggtggcag
     3421 ttcgtcaatg attgaggtgt cgaggtcagc atagaccgtc attgccagcg ttctcggaat
     3481 gggggtcgcc gtcataatga gctgatgcgg atagttattt tctttcgctc ctttttcccg
     3541 caaggtcagc cgttggtgta cgccaaaacg gtgttgttca tcaataatca ccaacgctaa
     3601 atggtgaaat tccacattgt cttggaacag ggcgtgagtg ccgataatca tctgcacatc
     3661 accgtttttg attgcctcaa gttgtgcggt tctcgccttg cctttcactt taccggcaag
     3721 ccagccaacc tcaatcccaa agggattaag ccagttggca aaattattgg cgtgctgttc
     3781 ggcaagaatt tcggtcggtg ccattaatgc cacctgcttg ccgttatcaa tcgctaatag
     3841 tgctgccaag gcggctacta aggttttacc tgaaccaaca tcgccttgta ccagtcgcat
     3901 catcgggtgt ggtttggcaa gatcttgctc aatatctgcg gttactcggc tttgtgcgtg
     3961 agtaggctta aaaggcaaac tgtcgagaaa acgctgtttt atatcggttt ggtaagtaag
     4021 tgcctctgca tattgctgtt ttacccctaa acggagttgc tgcatcgcta aattgtgtgc
     4081 cagcagttct tcaaaaatca gccgtttttg agcagggtgg tcaccttttt ccaagacttc
     4141 ggcagaaatc gtcggcggtg ggcggtgcaa taactgcaac gcttctttta ggctgtattt
     4201 gtgcggattg tattcatcag gcaacagctc ggcgactttc actttctcta ataaagcgag
     4261 ggcttgctcg gttaatttcc gcagcgaggc ttgttttaac ccctcagtgg tggagtaaat
     4321 cggggtcagg gtttcagcca gtacaagcgg ttgattatcc cgaataattt gatattcagg
     4381 gtggtgaatt tccgccataa atcgcccacg cttaatttcc ccaaaggctt ttacccgaac
     4441 gccggtggca aggctatttt tcattccagc attgaaattg aagaatttaa gcataatttt
     4501 gctggtgctg tcggagagtg taaccgataa aatcgggcgt ttaccaaact ggacttcggt
     4561 aagttgtaca tagccttcaa tggtggcaaa gctttccggg cgaacatcag caatcggggt
     4621 aatacgggtg cgatcttcat accgcattgg cagatgaaac agtaaatcct gcacattatt
     4681 aatgccgatt cgacttaact tttcggaaat tgccgctcct acgccggaaa gggcggttaa
     4741 tggcacgccg tctaataatt gttcactcat tatttatgcg gtttcaaatg ggttaaataa
     4801 acgcacatca caacggataa aatctttggt gttgcgggtg actaaaatca gattgtggta
     4861 tttggcggtt gccgcaatat aagcatcatt taaatccatt ttattgggaa tatggtactc
     4921 tgatgccaat agagcaatgt cgttattcac cacaaacgct tttgtttgat aagcctttaa
     4981 tacattattt tcaaaccaat catttaatcg gtctgattgt tctttgtctt tgcgggcttt
     5041 cagcaaaata cctgttttga tttcagataa cgtaatacag ctgatattgg tctggctagg
     5101 attaacccct ctcagccact ggcgaacatt gacatccatc ttgtcatttc ttaacttata
     5161 aagctcggaa actatattgg tatcaaaaag gtagcgatac attactcatc tccaaaatca
     5221 acaggtcttg gtttattgct tttgcggatt tcaaccaatg tttgttcaaa ctcttgccaa
     5281 aattcatcgc tcacatcctc attttcggaa gaggcgaata gctcttcaaa gcttggctcg
     5341 ctaggctttt tctgctgata atccatataa ctagacacca cattgacgat tttgccccat
     5401 ttggtaataa aaaccggctc tttttctgcc atttttaaca ctttgctggc attttgatta
     5461 aattctctgg aagtaatggt tattgccata tcgctctcca aaatgtagta a
//
LOCUS       DANLXS010000159         5508 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.160, whole genome shotgun sequence.
ACCESSION   DANLXS010000159 DANLXS010000000
VERSION     DANLXS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.160"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            88..1233
                     /gene="lldD"
                     /locus_tag="PXD71_002150"
     CDS             88..1233
                     /gene="lldD"
                     /locus_tag="PXD71_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HDL6292225.1"
                     /translation="MIISSANDYRRAAKRRVPPFMFHYADGGSYAEQTLRRNVSDLED
                     IALRQRVLKDMSQLDTGIELFGEKLSMPVTLAPVGALGMYARRGEVQAAKAADNKGIP
                     FTLSTVSICPIEEVAPAIKRPMWFQLYVLKDRGFMKNALERAKAAGCSTLVFTVDMPT
                     PGARYRDMHSGMSGPYKDIRRVLQAMVHPFWAWDVGIHGKPHTLGNVSNYMGKPIDLN
                     NYIGWLTDNFDPSISWKDLEWIREFWDGPMVIKGILDPEDAKDAVRFGADGIIVSNHG
                     GRQLDGVLSSAKALPSIADAVKGEIKILADSGIRNGLDVVRMLALGADCTMIGRSFVY
                     ALSAAGQAGVENLLDIFLKEMKVAMTLTSNAKISDIGRDALVDLSNL"
     gene            complement(1287..1556)
                     /locus_tag="PXD71_002151"
     CDS             complement(1287..1556)
                     /locus_tag="PXD71_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292226.1"
                     /translation="MLSEERFGFFYIFIFIYFSLLSLSIGYIRPELRTTYDYLEIENK
                     RYYVLSIHRNNIYVLGEKTKDNDEFLFFNQDTLKYYRINITKMPN"
     gene            complement(1631..2017)
                     /locus_tag="PXD71_002152"
     CDS             complement(1631..2017)
                     /locus_tag="PXD71_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292227.1"
                     /translation="MTNQKFINSSLTLAFFTLISFSTAYCYAWGIAAFHGYPWWHVEV
                     GNAGIARALAYVFGTFLTIFLFYLIGYALVNKVFKLHYFKYLGWLRVSVLVTIFSLPV
                     MISFYLFIGKVPMYLHLLYLGTTTSI"
     gene            complement(2270..3100)
                     /gene="fghA"
                     /locus_tag="PXD71_002153"
     CDS             complement(2270..3100)
                     /gene="fghA"
                     /locus_tag="PXD71_002153"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDL6292228.1"
                     /translation="MQQFENYRCFSGEQQVWQHFSASLNCEMKFAIYLPQSAKTQKLP
                     VLYWLSGLTCTEQNFITKSGFQRYADEHHLIVVAPDTSPRGVNVANDEAYDLGQGAGF
                     YLNATQGEWTTHYQMYDYIVSELPTLINAHFPTNGKQSIFGHSMGGHGALTIAFKNPG
                     QYQSVSAFAPIVAPTQVPWGQKAFLAYLGEDQTAWKAYDSVALLEKYRPSLPILIDQG
                     SQDSFLSDQLKPEIFCETADRLGIPYQFNLRDGYDHSYYFISSFIGEHIAFHAKHLKD
                     "
     gene            complement(3180..4238)
                     /locus_tag="PXD71_002154"
     CDS             complement(3180..4238)
                     /locus_tag="PXD71_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDL6292229.1"
                     /translation="MAYPIKSYATFSATTPLAPHSFERRELLEDDVLIDILYCGVCHS
                     DLHMARNDWGWTQSYPLVPGHEIIGRIIKVGKNAKKFQIGDLVGVGCMVDSCRECSPC
                     QHGLEQYCEVGNIGTYGGIDRFDGTPTQGGYSTCVVVSEDFVLKVSENLDTKAVAPLL
                     CAGITTYSPLRHWNVKAGSKVAVVGLGGLGHMAVKLAKAMGAEVTLFTRSLGKADDAY
                     RLGASRVVLSTDEKQMADVANTFDLIIDTVPYTHDLKPYIPTLALDGTLVLVGLVGEL
                     EQTINTVPMILGRRSISASVIGGIQETQEMLDFCAEHNIVPDVEMIDMQNINDAYERM
                     LKSDVKYRFVIDMGSLKG"
     gene            complement(4425..5360)
                     /locus_tag="PXD71_002155"
     CDS             complement(4425..5360)
                     /locus_tag="PXD71_002155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03571 family LLM class oxidoreductase"
                     /protein_id="HDL6292230.1"
                     /translation="MNTLSPIFSQVFGQNQLTLGLIAPFMGYQNHIPDNLTTIADNAR
                     LAENLGFSALWVRDVPFYDPHFGDVGQGLDPMVTLGFLSAHTQNIALGTAGLIAPLRS
                     PIHIAKMATSVDSLSHGRFLLGLSSGDRPVEYPAFGADFASRSERFRESIDLINTLTE
                     QDFPHFKGKHYADLTDNLDLLPKPKHRLPIVAIGRARQEMDWLANVPDAWIWHGVNPA
                     DTANIINTLADLRQKDTPTAFGYAQFVEVLDDKNAKAQLFNNIYLRGGAKGLADFWAK
                     EQEKGVKHLALNLKPTQRPADEVLTELAEYALTQF"
BASE COUNT         1590 a         1171 c         1222 g         1525 t
ORIGIN      
        1 ctcatatttt gcaaatagta gtaattctta tttttatttt aaagtagaat agccgcactt
       61 ttttcttaat gatttatgag gttttaaatg atcatttcat ctgcaaacga ttaccgccgt
      121 gcggcaaaac gtcgtgttcc cccttttatg ttccattatg cggatggcgg ttcttacgcc
      181 gagcagacct tacgtcgtaa tgtgagcgat ttggaagaca tcgccctgcg tcaacgtgtg
      241 ttaaaagata tgtcgcaact agacaccggc atcgagctgt tcggcgaaaa actgtctatg
      301 ccggtgaccc tcgcccctgt cggtgcatta ggaatgtatg cccgccgtgg cgaagtgcaa
      361 gcggccaaag ccgcagataa caaaggtatt ccgtttaccc tttccactgt ctctatttgc
      421 ccgattgaag aggttgcacc ggcaatcaaa cgcccgatgt ggtttcagct ctatgtgtta
      481 aaagatcggg gctttatgaa aaatgcattg gaacgagcca aagccgccgg ttgttccacg
      541 ctagtgttta ccgtagatat gccgaccccc ggcgcacgtt atcgtgatat gcactccgga
      601 atgagcggcc catacaaaga cattcgccgt gtgttgcagg caatggttca cccgttttgg
      661 gcgtgggacg tgggcattca cggtaaaccc cacacgttag gcaatgtgtc aaactatatg
      721 ggtaaaccga ttgatctgaa caactacatc ggctggctga ccgataattt cgacccatca
      781 atttcgtgga aagatttaga gtggattcgt gaattttggg acggcccgat ggtgatcaaa
      841 ggcattctcg acccagaaga tgctaaagat gccgtaagat ttggtgcgga cggtatcatc
      901 gtctccaacc acggtggtcg tcagttagat ggggtacttt cttctgccaa agccctgcca
      961 agcattgccg atgcagtaaa aggggagatc aaaatccttg ccgactctgg tatccgcaac
     1021 ggcttagatg tggtgagaat gctggctctc ggcgcagatt gtacgatgat cgggcgttcc
     1081 tttgtttacg ctcttagcgc cgccggtcaa gccggtgtgg aaaacctgct cgacatcttc
     1141 cttaaagaaa tgaaagtggc gatgacactt accagcaatg ctaaaattag cgacatcggc
     1201 agagatgctt tggtagattt aagtaatctt tagctcaaat agccacaatc cgcacgtttt
     1261 aggtgcggat tgttatttta gataggctaa tttggcattt tggtaatatt aatgcggtaa
     1321 tatttcaatg tatcttggtt gaaaaagaga aattcatcgt tatctttagt tttttcgccc
     1381 agcacataaa tgttattgcg atgaatagat aagacataat atcgcttatt ttcaatttca
     1441 agataatcat acgttgttct cagctcaggg cgaatgtagc caatactcaa agaaagtaag
     1501 ctaaaataga tgaaaataaa aatatagaaa aaaccgaagc gttcttcgct taacattttt
     1561 tgaatatcta agtggaatac gcggtggccc caatgtttgt gaaataataa tactgatagg
     1621 gtagttgtcc ctagatagag gtagttgtcc ctagatagag taggtgtaaa tacattggca
     1681 ctttgccaat aaacagatag aaactgatca ttactggcaa gctaaaaata gtaacaagaa
     1741 cactcacccg tagccagcca aggtatttaa agtaatgcag cttaaaaact ttattaacta
     1801 aagcgtatcc tatcaaataa aaaagaaaga tagttaaaaa tgtgccaaaa acgtaggcta
     1861 gtgcgcgagc tataccggca ttaccaacct caacatgcca ccacgggtag ccatgaaaag
     1921 cagcaatacc ccaagcataa cagtaagcgg tagaaaaaga gattaaagta aagaaagcga
     1981 gagtaagaga agagttgatg aatttctgat tagtcataac agtaaataga ttgcacctac
     2041 ttagttaaag atgaaacaca agaaagcaga ctgagtttag cttccaccca actcctttgt
     2101 ttagaggaga actttattaa agcacgactt ttgttgtatt tcaaaccttt gtttttatac
     2161 aaaacaaccg tgttgtaaaa aatgatcgta aactgaccgc ttgtaatgca ctctgaatat
     2221 atcgaaacat gataaaaatg ataaagcatg tttcgataat ttactgtctt tagtctttta
     2281 aatgttttgc atgaaaggct atatgttcac cgataaagct actaatgaaa tagtagctgt
     2341 gatcgtaacc atcacgtaaa ttgaattggt agggtattcc aagtctatct gcggtttcac
     2401 agaaaatttc cggctttaac tgatcgctca aaaagctgtc ttggctgcct tgatcgatta
     2461 gaattggcaa gcttgggcga tatttttcca gcaatgccac gctatcatac gccttccaag
     2521 cggtctgatc ttcgcctaaa tatgctaaaa atgctttttg cccccaagga acttgggttg
     2581 gggcgacaat cggtgcaaat gccgatacac tttgatattg ccccggattt ttaaatgcaa
     2641 tggttaaggc accatgtccg cccattgaat gcccaaaaat tgattgttta ccgttagtcg
     2701 ggaaatgagc attaatcaat gttggcagtt cgctgacaat gtaatcatac atttggtaat
     2761 gtgttgtcca ttcgccttgc gtagcattta ggtaaaatcc ggcaccctga cctaaatcat
     2821 aagcctcatc attagcgaca ttcacacccc tagggctggt atccggtgcg accacaatca
     2881 gatgatgttc gtcggcataa cgttgaaagc cagatttagt gatgaagttc tgctcggtac
     2941 aggttaaacc agaaagccaa tatagtacgg gtagcttttg tgttttagca gattgcggca
     3001 gataaatagc aaatttcatt tcacagttta agctggctga gaaatgttgc caaacctgtt
     3061 gttcaccgct aaaacagcgg tagttttcaa attgttgcat aatattcctt acaagcggtt
     3121 taattccgct gattttttgc aaaaataaag gcggaactca ccgcctttat attgtaatat
     3181 caacctttca aacttcccat atcaatcaca aaccgatatt tcacgtctga tttcagcatt
     3241 cgttcataag catcattgat gttttgcata tcaatcattt ccacatccgg cacaatgttg
     3301 tgttcggcgc agaagtcgag catttcttga gtttcctgaa tgccgccgat gacagaggcg
     3361 gaaatagagc ggcggcctaa aatcatcggt acggtgttga tggtttgctc caactcaccg
     3421 accaagccca ccagcaccaa tgtgccgtcc aacgctaagg ttgggatata aggtttcaag
     3481 tcgtgggtat aaggcacggt atcgatgatt aaatcaaagg tatttgcaac gtctgccatt
     3541 tgcttttcat cggtagaaag caccacacgg cttgcgccca aacggtaagc atcatcggct
     3601 ttacccagtg agcgagtaaa aagcgtaact tccgctccca ttgctttggc aagtttaacc
     3661 gccatatgcc ccagtccgcc aagccccaca acagcgactt ttgagccagc tttgacattc
     3721 caatgacgca ggggcgaata ggtggtaatg cctgcacaca aaagaggggc aaccgctttg
     3781 gtgtccaagt tttcagagac ttttaataca aaatcttcac tcaccaccac gcatgtgctg
     3841 taaccgcctt gtgttggtgt gccgtcaaag cggtcaatac cgccataagt gccgatgttg
     3901 cccacttcgc agtactgctc caaaccgtgc tggcaaggac tgcattcacg gcaggaatcc
     3961 accatacaac cgacaccgac caaatcgccg atttggaatt ttttggcgtt tttaccgact
     4021 ttgataatac gaccgatgat ttcgtgccct ggtacaagcg gatagctttg cgtccagccc
     4081 caatcattgc gagccatatg caagtctgaa tggcacacgc cacagtacaa aatatcaatc
     4141 aaaacatcgt cttcaagcaa ttcacggcgt tcaaaactgt gtggggcaag tggtgtggtt
     4201 gccgaaaaag tggcgtagga tttgataggg taagccattt ttggggttta aatggctgaa
     4261 atggcaatta ctatagtgcc gatgatttat tattgaaaat caagattttt gataaatcgg
     4321 tatcagttaa aatgataatt gggggttttc agactgactg aaaaattatt ttgcccattt
     4381 taaaaacaaa aacaccgccc tcatcacaaa agcggtgttt ttcatcaaaa ttgtgtcaaa
     4441 gcatactctg ccaattctgt aagcacttca tcagcaggac gctgagtggg ttttaaattg
     4501 agtgccaaat gtttcacgcc tttttcttgt tcttttgccc aaaagtctgc caagcctttt
     4561 gcaccaccac gcagataaat gttgttaaat aactgtgctt tggcgttttt gtcgtccaaa
     4621 acttccacaa attgagcata gccaaatgct gtgggggtgt ctttttggcg taaatcggca
     4681 agggtgttga tgatgtttgc ggtgtcggca ggattcacac cgtgccaaat ccacgcatca
     4741 ggtacattag caagccagtc catttcttgc cttgctcgtc caatcgccac aatgggcaag
     4801 cggtgtttgg gtttggggag caaatccaaa ttgtctgtta aatcggcata atgtttgcct
     4861 ttgaaatggg gaaaatcctg ctcggttaag gtgttaatca aatcaatgct ttcacgaaaa
     4921 cgctcgctac gagaggcaaa atccgcccca aatgcagggt attccacagg tctatcgcca
     4981 gaggataagc ccaataaaaa ccgtccatga ctcaaactat ccacacttgt cgccattttg
     5041 gcaatgtgaa tgggactacg caaaggggca atcagccctg ccgtgccaag ggcgatgttt
     5101 tgggtgtggg cggacaaaaa accaagtgta accatcgggt caagcccttg ccccacatcg
     5161 ccaaaatgag ggtcataaaa cggcacatct cgtacccaca atgctgaaaa gcccaagttt
     5221 tcagccagcc gtgcattgtc ggcaatggtg gtcaaattgt ctggaatgtg attttgatag
     5281 cccataaagg gggcaattaa gcccagtgtt aattgatttt gcccaaaaac ttgggagaaa
     5341 atgggggaaa gcgtgttcat tttggcgtac ctgtcttatt taagataagg tattttaagg
     5401 gcttttgagt ttgcttaaaa tgggataaat tgagaaagtg ttttgctaaa ttggcataaa
     5461 tagacgcttt caagctgact gaaaatttgc aaaattttta ataaatat
//
LOCUS       DANLXS010000160         5502 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.161, whole genome shotgun sequence.
ACCESSION   DANLXS010000160 DANLXS010000000
VERSION     DANLXS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.161"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            14..532
                     /gene="hutZ"
                     /locus_tag="PXD71_002156"
     CDS             14..532
                     /gene="hutZ"
                     /locus_tag="PXD71_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein HutZ"
                     /protein_id="HDL6292231.1"
                     /translation="MTTNRQEVLQNRLGPEIEELKQQIKTIMLATIDKDGVPNVSYAP
                     FVINNGEYQVLITTIARHARNLQEVPKVSLMLIEDESKSRQIFARRRLTFDATVRMIE
                     RTDEEWNNSIAALKARHGDLVDELSNMQDFKLFSFKPTAGLFVKGFGKAFDVGVDDLI
                     NVVHLDQGHQTA"
     gene            682..3294
                     /gene="pepN"
                     /locus_tag="PXD71_002157"
     CDS             682..3294
                     /gene="pepN"
                     /locus_tag="PXD71_002157"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDL6292232.1"
                     /translation="MQPKAKLRKDYRAPDFTITDIYLDFQLDPDRTFVTSKLTVVRKN
                     PEATTLRLDGHSFDFLSLKYNGEPFSAFQKDDESLTLNLADVPAERFELEIETALNPA
                     QNTSLQGLYKSGDGFCTQCEAEGFRQITYMLDRPDVLAKYTTKITASKSKYPYLLSNG
                     NHIAQGDLHDGRHWVEWQDPFFKPSYLFALVAGDFDLLQDKFITKSGREVTLEIYVDR
                     GNLDRAPWAMESLKRSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKF
                     VLAKPETATDTDYLDIEAVIAHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFTSD
                     LWSRSAKRIEDVRLLRTVQFAEDASPMAHPIRPEKVIEMNNFYTVTVYEKGAEIIRMI
                     HTLLGEEKFQQGMQLYVARHDGSAATCEDFVKAMEDASGVNLEQFRRWYSQSGTPELT
                     ISDEYDETRKAYRLHISQMTPATHDQMDKLNLHIPLKVALYGEQDGKRIPLQYELLTV
                     SDVLDVTAEHQTFEFHNVTQRPVPALLCDFSAPVRLDYNYTTEQLLTLLKHAENDFVR
                     WDAAQMLYTNELRENLSRYQQHQPLSFSEGLVNALTFVLDNSASLPELTALTLTLPKE
                     TEFAESFKTIDPQGIAVVREFMQHSIAHALQDKLLAVYHQNQCGEYQVVAEDIAKRAL
                     RNACLSYLAFTDLGNALVHKHYNNADNMTDTLAALQAATKAQLSCRDALLADFEQKWK
                     HDGLVMDKWFMLQATRPDDDVLSIVQNLMEHPSFNFNNPNRLRSLVGAFCGQNPKAFH
                     AIDGSGYRFLVDTLIKLNESNPQVAARLIEPLIKLSRYDNQRQTLMRRGLERLRELDN
                     LARDLFEKIEKALD"
     gene            complement(3354..3626)
                     /locus_tag="PXD71_002158"
     CDS             complement(3354..3626)
                     /locus_tag="PXD71_002158"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL6292233.1"
                     /translation="MVAKLFSVYGRVQGVAFRFFTLQEANRLGIKGYVRNRDDGSVEV
                     VAEADEQTIAQLENWLQQGSPAAKVERVIVSDYLGSERFKRFEVRY"
     gene            complement(3680..5314)
                     /locus_tag="PXD71_002159"
     CDS             complement(3680..5314)
                     /locus_tag="PXD71_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700110.1"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDL6292234.1"
                     /translation="MQSKFSLSLLTTSLVLGLSANVFAAKVPEGTTLAEKQEITINNG
                     FEPSSFDPHKIEGVPESQVAYQLFEGLVTKDSDGNIQPGVAESWENTPDYKTWTFKLR
                     QDAKWSNGDAVTAHDFVFAWQRLVNPLTASPYSSYLTYLNVENAQDIIDGKKKPEELA
                     VKAIDDHTFQVQLSQPVPYAVELATHSSLLPVHKATVEKHGDAWVQKGNLVGNGAYTL
                     ADHVINEKIVFERNKNYWNDKETVINKATFLAIANAATDVSRYRAGDLDMTNYALPPE
                     QFATLKKEIPNEVFTARTLSTYYYEINHKRAPFDNVKVREALNLALDRTIITDKVLGQ
                     GQTPTYVFTPPYIHEGEKIQQPAYSKDSMADRKAKALKLLEEAGYSKANPLKFTILYN
                     TNDNHKKIAIAVSSLWKQGTDGIVDVKLENQEWKTFLDTRRNANYDVSRAGWSADYNQ
                     ATTFVNYFLSNSSNNTAFYKSQAYDDAVAESYKATDAAGRAAAYAKAEEQLAKDYAII
                     PIYNYVNPRLVKPYVKGYEGKDPQDDILLRNLYIVK"
BASE COUNT         1608 a         1179 c         1096 g         1619 t
ORIGIN      
        1 attgaggtat tttatgacaa ccaaccgtca agaggtctta caaaaccgtt taggtccaga
       61 aattgaagaa ttaaaacaac aaatcaaaac tataatgctt gcaaccatag ataaagatgg
      121 tgtaccgaat gtgagctatg ccccttttgt aattaataac ggcgaatatc aagtcttaat
      181 cactactatt gcccgtcacg ctcgtaattt acaagaggtg ccaaaagtct ctttaatgct
      241 aattgaggat gaaagtaaga gccgtcaaat ttttgcccgt cgtcgcttaa cctttgacgc
      301 aacagtgcga atgattgaac gtactgatga ggaatggaat aatagcattg cagcacttaa
      361 agctcgtcac ggcgatttag ttgatgaact gtcaaatatg caagatttca agctctttag
      421 ctttaagcca acggcaggct tatttgtaaa aggatttggt aaagcctttg atgttggtgt
      481 tgatgactta attaatgtcg ttcatttaga ccaaggccac caaactgcct aattgcgaaa
      541 taaactcacg gctataaata atcctattta tagccgtttt tattgcgaca agcggtcaaa
      601 ttttttggaa aatttaccgc tattttagta caataacgac actattctat gagctaaacg
      661 caactaaaaa aaggaaatcc tatgcaacct aaagcgaaac tcagaaaaga ttatcgagct
      721 cccgatttca ctattactga tatttatctt gacttccaac ttgacccaga tcgcactttt
      781 gtcacctcaa aactaactgt cgtgcgtaaa aatcctgagg cgacaacact tcgtttagac
      841 ggtcatagtt tcgatttcct ttcgcttaaa tataacggcg agccgttttc cgcctttcaa
      901 aaagatgatg aatctttaac cttaaattta gccgatgtgc ctgccgagcg gttcgagctt
      961 gaaattgaaa ccgcattaaa tccggcacaa aacacctcgt tacaaggttt gtataaatcg
     1021 ggcgacgggt tttgcaccca gtgtgaggct gaaggtttcc gccagattac ctatatgctc
     1081 gaccgccctg atgtacttgc aaaatatacg acaaaaatca ccgctagtaa gagcaaatac
     1141 ccgtatctgc tttctaacgg caaccacatt gcccaaggcg acttgcacga tggcagacac
     1201 tgggtggaat ggcaagatcc gttctttaaa ccaagctatt tatttgcctt ggttgcgggg
     1261 gattttgact tactccaaga taaattcatc accaaaagcg gacgtgaagt cacgcttgaa
     1321 atttatgtcg atcgtggaaa tttagaccgt gcaccttggg cgatggagag cttaaaacgt
     1381 tcaatgaaat gggatgaaga gcgtttcggt ttggaatacg atttagatat ttatatgatt
     1441 gttgccgttg atttcttcaa tatgggagca atggaaaaca aagggttaaa tattttcaat
     1501 tccaaatttg tattggcaaa accggaaacc gcaaccgata cggattattt agatattgaa
     1561 gcggtgattg cccacgaata cttccataat tggacgggca accgcattac ctgccgtgat
     1621 tggttccaac taagcttaaa agagggctta accgtcttcc gtgatcagga atttacctcc
     1681 gatttatggt ctcgttcggc aaaacggatt gaagatgtgc gtttactgcg taccgtacaa
     1741 tttgccgaag atgccagccc gatggctcac ccgattcgcc cggaaaaagt gattgaaatg
     1801 aataatttct acaccgtgac tgtgtatgaa aaaggggcgg aaattattcg tatgattcac
     1861 accctgttag gcgaagaaaa attccagcaa ggtatgcaac tttatgtggc aagacacgat
     1921 ggttctgcgg caacttgtga agatttcgtt aaagcaatgg aagatgcttc aggcgttaat
     1981 cttgaacaat tccgccgttg gtatagccaa tccggcacgc ctgagctcac catttccgat
     2041 gaatatgatg aaacccgcaa ggcataccgc ttgcatattt cacaaatgac accggcaacc
     2101 cacgatcaga tggataaact caatctgcat attccgctta aagtagcgtt atatggtgaa
     2161 caggacggta aacgcattcc gttacaatat gaattattga ccgtaagcga tgtgttagat
     2221 gtaaccgcag aacaccaaac ctttgagttt cataatgtaa cacaacgccc ggtgcctgca
     2281 ttactgtgtg atttctctgc accggtacgg ttagattaca actataccac cgagcagctt
     2341 ttaaccttac tcaaacacgc tgaaaatgat tttgtacgct gggatgcagc tcaaatgctc
     2401 tataccaatg aactgcgtga aaatttaagc cgttatcagc aacatcaacc actcagcttc
     2461 tcagaagggt tggtgaatgc cctaacgttc gtgttggata actcagcaag cctgccggaa
     2521 ctaactgcat taaccctcac attgccaaaa gaaaccgagt ttgcagaatc gttcaaaacc
     2581 attgacccac aaggtattgc ggttgtgcgt gaatttatgc agcattccat tgctcacgcc
     2641 ttgcaagata agttattagc ggtatatcac caaaaccaat gcggggaata tcaagtcgtg
     2701 gctgaagata ttgctaaacg agccttgcgt aatgcttgct taagctattt agcctttact
     2761 gatttaggca acgcgttggt gcataagcat tacaataatg ccgataatat gactgatacg
     2821 cttgctgcat tacaagccgc aaccaaagcc cagctaagtt gccgtgatgc attattagcc
     2881 gattttgagc aaaaatggaa acatgatggt ttagtgatgg ataaatggtt tatgctacaa
     2941 gccacccgac cggacgacga tgtgttatcc atcgtgcaaa atttaatgga acacccaagt
     3001 tttaacttta ataacccgaa ccgtttacgt tcattagtag gtgcattctg cggtcaaaac
     3061 ccgaaagcct tccacgccat tgacggttca ggctatcgtt tcttggtaga tacgctgatt
     3121 aaacttaacg aaagtaaccc acaagttgct gctcgactta ttgagccgtt aattaaactc
     3181 tcacgctacg ataaccaacg ccaaaccttg atgcgtcgtg gtctagaacg cctgcgtgag
     3241 ctggataatt tagcacgaga tctgtttgag aaaattgaaa aagcgttaga ttaaaccaag
     3301 caatacaagc ggtgtcgttt gcaaattttt ttacaaatga caccgcttgt tagttaatac
     3361 cttacctcaa accttttaaa cctttctgag ccaagataat cgctcacaat tacccgttcc
     3421 acctttgcag caggtgagcc ttgttgtaac caattttcca gttgagcgat tgtctgttca
     3481 tccgcttcag ctaccacttc taccgagccg tcatcacgat ttctgacata gccttttatg
     3541 cctaatcgat tcgcttcctg caaggtaaaa aaccgaaaag cgacaccttg aacccgtccg
     3601 taaaccgaga ataattttgc gaccattctt cccccataaa aataggctgc ttatttttca
     3661 ataaacagcc cataatgtct tatttcacga tgtagaggtt acgcagtaaa atatcatctt
     3721 gcggatcttt accctcatag cctttcacgt aaggtttcac caaacgtggg tttacatagt
     3781 tataaatcgg aataatcgca taatctttgg ctaactgctc ctctgctttt gcataagcag
     3841 ctgcacgacc agctgcgtct gtcgctttgt aagattctgc taccgcatca tcataagctt
     3901 ggctcttata aaacgcagta ttgttgctgg agttcgataa gaaatagttc acaaaggtgg
     3961 ttgcttggtt atagtctgcc gaccaacctg cacgagaaac atcataattt gcattgcggc
     4021 gggtatctaa gaatgttttc cactcttggt tttctaattt cacatcaaca atgccgtctg
     4081 tgccttgttt ccataatgat gaaactgcaa tcgcaatttt cttgtggttg tcgttggtat
     4141 tataaagaat agtaaatttt aacgggttcg ctttactgta accggcttct tccaataatt
     4201 tcaacgcttt ggcttttcta tctgccattg aatctttgga ataagcaggt tgctggattt
     4261 tttcaccctc gtgaatataa ggcggtgtaa atacataagt tggtgtttga ccttgtccta
     4321 gcactttatc ggtaataatg gtgcgatcta aagctaagtt taacgcctca cgcactttca
     4381 cattatcaaa cggtgctcgt ttatggttaa tttcatagta ataagtagaa agggttcttg
     4441 ccgtaaacac ttcgtttgga atctcttttt tcaaggttgc aaactgctcc ggtggaagag
     4501 catagttggt catatccaaa tcaccggcac ggtaacgtga aacatcagtt gcggcattag
     4561 caatggctaa gaaagtcgct ttattaatca cggtttcttt atcattccaa taatttttat
     4621 tacgctcaaa cacgattttt tcattaataa cgtgatcggc taaggtataa gccccgtttc
     4681 caaccagatt gcctttttgc acccacgcat cgccgtgttt ctcaacggtt gctttgtgta
     4741 caggtaataa agaagagtgt gttgccagct caactgcata cgggacaggt tggcttaact
     4801 gcacctggaa agtatgatca tcaatcgctt taactgctag ttcctccggt tttttcttgc
     4861 cgtcaataat atcttgagca ttttctacat tgagataagt taaatagctt gaataaggtg
     4921 aagccgtaag tggattaacc aaacgctgcc aagcaaatac gaaatcgtga gcagttacgg
     4981 catcaccgtt tgaccattta gcgtcttggc gtaatttgaa cgtccacgtt ttgtaatccg
     5041 gtgtattttc ccaactttct gccacacccg gctgaatatt gccgtcagaa tctttcgtta
     5101 ccaatccttc aaataactga taagcaactt gcgattccgg cacgccctca attttatgtg
     5161 gatcaaaact tgatggctca aaaccattat taatcgtaat ttcttgtttt tctgcaagcg
     5221 ttgttccttc aggcactttg gctgcaaaca catttgccga taagcctaac actaatgagg
     5281 ttgttaataa ggaaagcgag aattttgact gcatatttga tctccattga tgaatattgg
     5341 cttgcaacca gccgatttgt tctaattaat ttttttaagt ctaggcatta ttattttgat
     5401 tgcaaggaat ttttttaaaa aatgtgatag atatcacctt tcaatgcaaa aattgcataa
     5461 aaaaatcccg aagaaaatag ctctataaac tctgataatg gc
//
LOCUS       DANLXS010000161         5471 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.162, whole genome shotgun sequence.
ACCESSION   DANLXS010000161 DANLXS010000000
VERSION     DANLXS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.162"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..2580
                     /gene="topA"
                     /locus_tag="PXD71_002160"
     CDS             <1..2580
                     /gene="topA"
                     /locus_tag="PXD71_002160"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL6292235.1"
                     /translation="SPAKAKKINQYLGSNYVVKSSVGHIRDLPKSGSDEKTEKAKPIS
                     TKGLTAAEKAKIKAEKERVALVKRMGIDPYNGWKANYQILPDKEKVVSELKSLAKKAD
                     HIYLATDLDREGEAIAWHLREIIGGDDSRFSRVVFNEITKDAIKKAFDDPQPLNIDRV
                     NSQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQSEEFW
                     TILANTQINRKKLPLELTTYKGKKFSASNEAEANVAVRALEQSDFIVSNIEAKETSSR
                     AAAPFITSSLQRAASTRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSREALAMARG
                     YIQDNFGANYLPEKANVYRSKDNAQEAHEAIRPSDVNVKMADLKGMDKDAERLYDLIW
                     RRFLACQMLPARYDSTAVTVSAGDYELKANGRVLRFDGWTKVLPVQGKNPEDQELPAV
                     ELHQKLTLDEIVPSQHFTKPPARFNEASLVAELEERKIGRPSTYASIISTIQERGYVR
                     TENKRFYVEKIGEVVTDRLNESFKDLMNYDFTANMEEVLDQIAEGSKNWKEELNTFFK
                     HFSEELSQAELDELEGGMKPNSLVPTDIDCPTCGRKMAIRTASTGVFLGCTGYALPPK
                     ERCKTTMNLIPEAEFFNVLDSDSETKALMARKRCPKCDSAMDSYVIDAERKLHVCGNN
                     PNCDGHIIEQGSFKIKGYDGPVVECDKCGSDMHLKLGRFGKYMGCTSCDNTRKILKNG
                     EVAPPKEDPIAFPELKCEKADAYFVLRDGASGVFMSAHNFPKVRESRAPKVVELAQYR
                     ERLPEKLQYLADAPQQDPEGNEAIIRFSRKEKRQYVTSEKNGKATKWIVDYLDGKWVE
                     RKK"
     gene            complement(2630..3568)
                     /gene="yvcK"
                     /locus_tag="PXD71_002161"
     CDS             complement(2630..3568)
                     /gene="yvcK"
                     /locus_tag="PXD71_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDL6292236.1"
                     /translation="MENTDIIPFKPTRHHYLNQLNAVVALGGGHGLGRLLSALSFLKE
                     RLTGIVATTDNGGSTGRIRSQQGVIAWGDLRNCLNQIIVKPTIASRLFEYRFAGTGEL
                     AGHNLGNLILTALENMQIRPLEGINLVRELLHVRSQLIPMSEIPVHLIAQLHSGESVF
                     GEVQIDSLAETPQHLALDTMVVATPEAISAIQSAELILLGPGSFFTSIMPNLLVAGIA
                     NSIAQSKARVVFIDNIGQEHGPAGQLSLNQRIEWMENCIGQARINAVITTDDAGSELP
                     EHIQILRKNLSANDVYYRHDRNKLSKAIDELLAQNI"
     gene            complement(3582..4658)
                     /locus_tag="PXD71_002162"
     CDS             complement(3582..4658)
                     /locus_tag="PXD71_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL6292237.1"
                     /translation="MFQMLQDWYRKKFTDPQIVVLFSILLIGFGIIYFFSDLLMPLLV
                     ALVFAYLLEWPIRFLSSKLKLPRTLSVILVLGGFIALLSFLGVVLLPSLWNQAVTFIQ
                     DLPSMFNLLNAWLQALPEHYPELVDYATLDSIVNTAKSNILSMGESLLTLSINSIISL
                     VGLGIYTFLVPLMVFFLLKDKIVLMRSFSKVLPQNRRLATRVWFEMQQQIANYIRGKF
                     LEILIVGVVTYIIFLFFDLRYPLLLSVAVGISVLVPYIGAVLVSIPVMLIALFQFGLS
                     PDFYYLMLAFIISQLLDGNLLVPFLFSEAVNLHPLTIIIAVLIFGGLWGFWGVFFAIP
                     LATLVKAVLQALPSAQSDAIILEK"
     gene            complement(4689..5444)
                     /locus_tag="PXD71_002163"
     CDS             complement(4689..5444)
                     /locus_tag="PXD71_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6292238.1"
                     /translation="MKKEEVGHHFLARLGKTRLRPGGKLATDWLIANGDFDKNKKVLE
                     VACNMGTTAIQLAQQFECQIVGIDLDEDALEKARKNIKENHVEHLIQVQRANATKLPF
                     DDESFDIVINEAMLTMLPLEAKEKAIREYFRVLKPNGFLLTHDIVIQDKDSDTLLAEL
                     RDAINITVSPLAKADWKSLFQRCGFRNVDTFSGEMSLLSPKGLIHDEGVLGAMKIIGN
                     ALKPENRETFNKMYKLFNDPDKKLGFIAVCSQK"
BASE COUNT         1720 a         1040 c         1194 g         1517 t
ORIGIN      
        1 tctccggcta aggcaaaaaa aattaaccaa tatcttggta gtaattatgt ggtgaaatcg
       61 agtgtggggc atattcgtga tttaccgaaa agtggtagtg atgaaaaaac ggaaaaagcg
      121 aaaccgattt caaccaaagg gctgaccgct gccgaaaaag ctaaaatcaa agcagaaaaa
      181 gagcgtgttg cattggttaa acgtatgggt atcgacccct ataatggctg gaaagctaac
      241 tatcagattt tgccggataa agagaaagtg gtttccgagc taaaatcgtt ggcaaagaaa
      301 gcagatcata tctatctggc aaccgacttg gatagagaag gggaggcgat tgcgtggcat
      361 ttgcgtgaaa tcattggtgg tgatgacagc cgttttagcc gagtggtatt taatgaaatc
      421 accaaagatg cgatcaaaaa ggcttttgac gatccgcaac cgctcaatat tgaccgtgta
      481 aattcccagc aaacccgccg ctttttagac cgtgtagtgg gctttatggt gtcgccatta
      541 ttatggaaaa aagtagctcg tggtttatcg gcagggcgtg tgcaatcggt tgccgtaaaa
      601 ttactggttg agcgtgagcg tgaaatcaag gcgttccaaa gcgaggagtt ttggactatt
      661 cttgccaata cgcaaattaa tcgtaaaaaa ttaccgcttg aattaactac ttataaaggt
      721 aaaaaattct cggcaagcaa tgaggctgaa gcaaatgttg cagtgcgtgc tttggagcag
      781 agcgatttta ttgtttctaa tattgaagcc aaagaaacca gctctcgagc tgctgcacca
      841 tttattacct catcgttaca acgtgccgct agcactcgtt taggttttgg ggttaaaaag
      901 acgatgatgt tagcccaacg gttatatgaa gcgggttaca tcacctatat gcgtactgat
      961 tcaaccaacc ttagccgtga agcattagca atggctcgag gttatattca ggataatttc
     1021 ggggcgaatt atttgccgga aaaagcaaat gtgtatcgca gtaaagataa tgcccaagaa
     1081 gctcacgaag ccattcgccc gtcggatgtg aatgtcaaaa tggcggattt aaaaggaatg
     1141 gataaagatg ccgagcgttt atacgattta atttggcgtc gtttcttggc gtgtcaaatg
     1201 ttgcctgctc gttatgattc taccgcagtc acagtgtctg ccggagacta tgaactcaaa
     1261 gcaaacgggc gtgtactgcg ttttgacggc tggacgaaag tactgccggt gcaaggcaaa
     1321 aatccggaag atcaggaatt accggcggtt gaactgcatc aaaaattaac gcttgatgaa
     1381 attgtgccaa gtcagcattt tactaaaccg cctgcacgtt ttaatgaggc gtctttggtt
     1441 gctgagttag aagagcgaaa aattggccgc ccttcaacct atgcttcaat tatttcgacg
     1501 attcaagaac gtggctatgt tcgcaccgaa aacaagcgtt tttatgtgga aaaaatcggt
     1561 gaagtagtga cggatcgcct caacgaatca ttcaaagatt taatgaatta tgatttcact
     1621 gccaatatgg aagaggtgct cgaccaaatt gcagaggggt ctaaaaattg gaaagaagag
     1681 ttgaatactt tctttaaaca tttctctgaa gagttgagcc aagcagaatt agatgaactt
     1741 gaaggcggaa tgaaaccaaa cagccttgtg ccaaccgata ttgattgccc gacttgtgga
     1801 cgaaaaatgg caattcgcac cgcttccaca ggggtatttt taggttgcac cggctatgct
     1861 ttaccgccaa aagagcgttg caaaacgaca atgaatttaa ttccagaggc ggaatttttc
     1921 aatgtgttag attcggattc tgaaaccaaa gccctaatgg ctcgcaagcg ttgcccgaaa
     1981 tgcgattcgg caatggatag ttatgtgatt gatgctgaac gtaaattaca cgtttgtggt
     2041 aataatccaa attgtgatgg tcatattatt gagcaaggtt ccttcaaaat taaaggctat
     2101 gacggacctg tagtggaatg cgataaatgc ggttcagata tgcacttaaa actgggtcgt
     2161 ttcggcaaat atatgggctg cacaagctgc gataacaccc gtaaaattct gaaaaacggg
     2221 gaagttgctc cgccaaaaga agatcctatt gcttttcctg aattaaaatg tgaaaaagcc
     2281 gatgcctatt ttgtattaag agacggagca agtggggtgt ttatgtcagc tcataacttc
     2341 ccgaaagtgc gtgaaagccg tgcaccaaaa gtggtagagt tagctcaata tcgtgaacgt
     2401 ttgccggaga aattgcagta tcttgccgat gctccgcagc aagatccgga aggaaacgag
     2461 gcaattatcc gttttagtcg taaagagaaa cgccaatatg tgacttctga gaaaaacggc
     2521 aaagccacca aatggattgt tgattatcta gacggtaaat gggttgagcg taagaaataa
     2581 agcttaaaag acaagcggta aaattttcta aaaattttac cgcttgcttt tagatgttct
     2641 gagcgagtag ttcatcaatt gctttactta atttattgcg atcgtgtcgg taataaacgt
     2701 cattcgcaga taagtttttt cgcaagattt gaatatgttc gggaagttca gaaccggcat
     2761 catcagtcgt aattaccgca ttaattctag cctgtccgat acagttctcc atccattcaa
     2821 ttcgctgatt aagtgatagt tgcccagctg ggccatgttc ttgtccgata ttgtcaataa
     2881 atactactcg ggctttgctt tgagcaatag agtttgctat gcctgccacc agcaaattcg
     2941 gcataatact ggtaaaaaag ctacctgggc ctagcagtat taactcagca ctttgaatag
     3001 ctgaaatggc ttctggtgta gcaacaacca tagtatctaa cgctaaatgt tgtggtgttt
     3061 ctgctaaaga atcaatttgc acttcaccaa atactgattc gcctgaatgc aactgtgcaa
     3121 ttaaatgcac cggtatttca gacattggga taagttgcga tcttacgtgc aataattctc
     3181 gcaccaaatt aatcccttct agcggtcgga tttgcatatt ttcaagggcg gtgagaatta
     3241 aattgcctag attatgtcca gcgagttcgc ctgtgccagc aaaacgatac tcaaacaacc
     3301 gagaagctat cgttggttta acgataattt gatttaaaca gttgcgtaaa tctccccaag
     3361 caatcacccc ttgttgtgaa cgtattctac cggttgagcc tccattatcg gtagtggcaa
     3421 caatgccggt aagccgttcc tttaaaaaag agagcgcgga gagcaatcga cctaagccgt
     3481 ggcccccacc tagtgcaaca actgcattga gttggtttaa atagtgatgt cgggtaggtt
     3541 taaaaggaat aatatctgta ttttccatct tgggatttcg tttatttttc taaaataatg
     3601 gcatctgact gggctgaagg caatgcttgt aatacagctt taaccaaagt cgctaaagga
     3661 atcgcaaaaa acactcccca aaagccccat aaaccaccaa agatgagtac ggcaataata
     3721 attgtcagag ggtgtagatt tactgcttca gaaaataaaa agggtacgag taaatttccg
     3781 tctagcaact gactaataat aaatgcgagc attaaatagt agaaatccgg cgataacccg
     3841 aactggaata gagcaattaa cattaccgga atgctgacta aaaccgcacc gatgtaggga
     3901 actaacactg aaatccctac cgctacagaa agtagtaacg gatagcgtaa atcaaaaaag
     3961 aggaaaataa tataagtgac aaccccaaca attagaattt ctaaaaattt acctcgaata
     4021 tagttggcaa tttgttgttg catttcaaac catactcgtg ttgcaagtcg acggttttgc
     4081 ggtaatactt tactaaaaga acgcattaaa acgattttat ctttcagtaa gaaaaagacc
     4141 atgagtggga ctaagaaggt gtaaatgcct aagccaacta agctgatgat agagttaatc
     4201 gagagtgtta gaagtgattc tcccatactc aaaatattac ttttagcggt attcacaatg
     4261 gaatctaggg tcgcataatc tactaattca gggtaatgct ccggtagggc ttgtaaccaa
     4321 gcattgagca gattaaacat cgaaggtaaa tcttgaataa aggtaactgc ttgattccaa
     4381 aggctgggta gtagtactac gcctaaaaaa gataataaag cgatgaaacc tcctagtact
     4441 agaatgacac tcaaagttcg aggtaatttg agtttgctcg acaaaaaacg gattggccat
     4501 tccaataaat aggcaaacac aagtgcaacc agaaggggca taagcagatc gctaaaaaaa
     4561 tagataatac cgaaaccgat caatagaata ctaaacagca caactatttg cgggtcggta
     4621 aatttttttc gataccaatc ttgcaacatt tgaaacatat ggattgcctt taaattatgc
     4681 gatgaaaatt atttctggct acagactgca atgaacccta gctttttgtc cgggtcatta
     4741 aatagtttgt acattttatt aaaggtttcg cgattttctg gttttaatgc attgccgata
     4801 attttcatgg cgcctaaaac accttcatca tgaatcagac ctttagggga aagtagagac
     4861 atttctccgg aaaaggtatc aacattacgg aaaccacaac gttggaataa tgatttccag
     4921 tctgctttag caagtggaga gacagtgata ttgattgcat cacgcaactc ggctaataaa
     4981 gtatcggagt ctttatcttg gataacaata tcgtgcgtta ataaaaagcc atttggtttt
     5041 aatacacgaa aatattcacg tattgccttt tctttcgctt ctagaggaag cattgtcagc
     5101 atggcttcat taattacaat atcaaaactt tcgtcgtcaa atggcagttt tgttgcgttg
     5161 gcgcgctgta cttgaattaa atgctcaaca tggttttctt tgatattttt acgtgctttt
     5221 tcaagcgcat cttcgtctaa atcaatacca acaatttggc attcaaattg ttgagctaat
     5281 tggattgcag tagtccccat gttacaggca acttcgagca cttttttatt tttatcaaaa
     5341 tccccgtttg caattaacca atcagttgct aatttccccc cggggcgtaa acgagttttg
     5401 cctaaacgag ctaaaaaatg atgaccaact tcttcttttt tcataaaaac ctctggattt
     5461 gcaaaatacg t
//
LOCUS       DANLXS010000162         5463 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.163, whole genome shotgun sequence.
ACCESSION   DANLXS010000162 DANLXS010000000
VERSION     DANLXS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.163"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            89..223
                     /locus_tag="PXD71_002164"
     CDS             89..223
                     /locus_tag="PXD71_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/trimethylamine N-oxide reductase
                     NapE/TorE"
                     /protein_id="HDL6292239.1"
                     /translation="MRSELLKTLLLSGLILPLLMAFAVGAYGFIVWMLQLFVIGLPGA
                     "
     gene            227..1390
                     /gene="torC"
                     /locus_tag="PXD71_002165"
     CDS             227..1390
                     /gene="torC"
                     /locus_tag="PXD71_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDL6292240.1"
                     /translation="MLGSIKALFTRPATKIGLGVLVGIGFIAGAISWHQFNKAMDHTS
                     TEEFCVSCHSMQQPLEELKKTAHWSNKSGVTATCSSCHLPHDYTEKYARKVQASREAW
                     AELSGKYKEEGSFEKHRLEMAEREWARFAANGSKECKACHSYERMNFDKMGEAARKAM
                     KPAAEKDQSCMDCHKGIAHHLPKADENALKSKFDQFVNKSPNQGKSYYTKGNAELFLD
                     EGLSQAIGQLETAVPVAFVQAGKNADLVELTMWRKEKGFGRIWYHDFGKNITDAVLTK
                     EFMANQPKFEVLETKEDPITGLNWQKVKLQAWIGKAQLLDDVTPIWANAENQYKTQCS
                     TCHRQPDVAHFDSNSWIGLFNGMVGFTNMDKQTGKEVLRYLQMHASDSEEAKH"
     gene            1490..3955
                     /gene="torA"
                     /locus_tag="PXD71_002166"
     CDS             1490..3955
                     /gene="torA"
                     /locus_tag="PXD71_002166"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL6292241.1"
                     /translation="MQQSRRQFLKNMSVMAAAVTMPNFLVPRNVFASEDLSQWKVSGS
                     HWGAMRAKIENGRVAEIKPFEFDKHPTEMLNGIKGLIYSDSRIRYPMVRLDWLKNRHN
                     SNTAQRGDNRFVRVTWDEALDLFYQELERVQKEYGPWALHTANVGWRSTGQFHSCGNH
                     MIRAIAMHGNSVGTAGDYSTGAGQTILPYVLGSTEVYSQGTSWEIILKESENIIFWAS
                     DPVKNLQVGWNCETHEAYAYLEKLKEKVAAKGVNVICVDPVKSKTQNFLGCEQQYINP
                     QTDVPFMLALAHTLYTENLYDKKFLDMYTVGFEKFLPYLLGETEDKVAKTPEWAAEIC
                     GISADKIREFARMLAGKRTQFIFGWAIQRQQHGEQPYWMGAVLASMLGQIGLEGGGIS
                     YAHHYSSIGIPESGAAMPGAFPLNIDEGQTPKYDNKDYKGYSDTIPVARMTDSLLHAG
                     ETIDYNGKKVTYAPFKMAIFTGCNQWHRHSERNKMKQAFQKLETIVSINYSWTATCRF
                     SDIVLPACTPFERNDIDAYGSYSNRGVIAMHKLVDPLYDSRSDFEIFRDLCRRFGKEK
                     EYSRGMDEMQWVEKLYKDCRRENRGKFEMPEFAEFWQKGYVLFPEGKPWVRHADFRED
                     PELHALGTPSGFIEIYSNKIASFGYDDCKGHPMWFEKAERSHGGKNSDKFPYWLQSVH
                     PDKRLHSQLCESKELRETYSVQGREPLYINPQDAAKHGIQDGDLVRVFNDRGQAIVGA
                     VLSDNFPTGVVRLQEGAWYSPLDESVGAIDTYGDPNTMTLDIGTSKLAQAVSANTCLV
                     NIEKFIGNAPAPNGFNGAIEVTA"
     gene            3955..4545
                     /gene="torD"
                     /locus_tag="PXD71_002167"
     CDS             3955..4545
                     /gene="torD"
                     /locus_tag="PXD71_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDL6292242.1"
                     /translation="MTLAISREERQFIYGWFNAMLARELSDEQLNALQAGEFDDFFAY
                     LSELGFAEQVAKFQAEVTACRQLEFPRLELAADFAQLFLLEGNLSAIPYASAYLSGEA
                     LAKNLAEMDRLLGEFGLQVNRETKEPSDHLCVYLTLFDRLLAEKPLETQQQFLQNQLM
                     SWLPAWFAKAKTVKTNTGVYPALLRLFEQVLLKEQR"
     gene            4685..5374
                     /locus_tag="PXD71_002168"
     CDS             4685..5374
                     /locus_tag="PXD71_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL6292243.1"
                     /translation="MQNNKTTYLNWYDLCVLTLILFGEAIYTSTQQYFITTEMQGNFE
                     ENLTFSNEQNYWALLTQLALLLIAMGYLKWRNFDFSRWVIRPSWLAFFSGIGLFILLS
                     LAMDLYFETLYGVYNLVYPEPFWAVLREVNISTVLYAILNGFYEEIFFLGICLAVKPE
                     YLKWAVIYSLIIRVSFHTYQGMESALGLGIWLGLIIYLLYSRSKQKNLFPFWVAHSIA
                     DILGLSLLAYF"
BASE COUNT         1565 a         1188 c         1320 g         1390 t
ORIGIN      
        1 cttctttata tttttgattg atatcaaggc aaattgtttc tgaagataaa ataattaaca
       61 cttattagtt aataaaaaag gagttgaaat gcgttcagaa ctattaaaaa cgcttttgct
      121 atcagggctg attttaccgc tattgatggc gtttgccgta ggagcttacg gctttattgt
      181 gtggatgtta caactgtttg ttattggttt accgggagcg tagaaaatgt taggttcaat
      241 caaagcatta tttactcggc ctgcaaccaa aatcgggttg ggagtgttag ttggtatcgg
      301 ttttattgcc ggagcaatta gctggcatca atttaataaa gcgatggatc ataccagcac
      361 tgaagagttt tgcgtgagtt gccactcaat gcagcagccg ttagaggagc ttaaaaaaac
      421 cgcccactgg tcgaataaaa gcggggtaac ggcaacctgt tcaagctgcc acttaccgca
      481 cgattatacc gaaaaatatg cccgcaaagt gcaggcaagc cgtgaggcgt gggcagaatt
      541 atccggcaaa tataaagaag aaggctcatt tgaaaaacac cgcttagaaa tggcagagcg
      601 tgagtgggca agatttgccg ccaatggttc aaaagagtgt aaggcttgcc acagctatga
      661 gcgaatgaat tttgataaaa tgggcgaagc agctcgtaag gcaatgaaac cggctgcgga
      721 gaaagatcaa agttgtatgg attgccacaa aggcattgct caccacttac cgaaagccga
      781 tgaaaatgca ctgaaatcga agtttgatca gtttgtgaat aaatccccaa accaaggcaa
      841 atcttactac accaaaggga atgccgaatt atttctagac gaaggtttaa gccaagctat
      901 cgggcaatta gaaaccgccg tgccggtggc atttgtgcaa gcgggcaaaa atgcggattt
      961 ggttgagctg acaatgtggc gtaaagagaa aggtttcggt cgcatttggt atcacgattt
     1021 cgggaaaaac attaccgatg cggttctaac caaagagttt atggcaaatc agccgaaatt
     1081 tgaggtgttg gaaaccaaag aagatccgat taccggcttg aattggcaaa aagtgaaact
     1141 acaagcgtgg attggcaaag ctcaattatt agacgatgtt accccaatct gggcgaatgc
     1201 agaaaaccaa tacaaaaccc aatgtagcac ttgccacaga cagcctgatg tcgcccactt
     1261 cgattccaac agctggatcg gcttattcaa cggaatggtc ggctttacca atatggataa
     1321 acagaccggc aaagaggtgc tacgttattt acaaatgcac gcctccgata gtgaagaagc
     1381 gaagcattaa taatattact cccctcttta ctaaagaggg gaattaatcg agcgttgaat
     1441 aacattctct gtactagctc acgtttttac aaataaagaa ggacaagata tgcaacaatc
     1501 tcgccgtcaa tttttaaaaa atatgtctgt gatggctgcc gctgtcacaa tgccgaattt
     1561 cctcgtgcct cgcaatgtgt ttgcgagtga agacctcagt caatggaaag tttccggctc
     1621 tcactggggg gcaatgcgtg ccaaaattga gaacggacga gtggcagaaa tcaaaccgtt
     1681 cgagtttgat aaacacccga ccgaaatgct caacggcatt aaaggcttaa tttacagcga
     1741 ttcccgtatc cgctacccga tggtgcgttt agattggctc aaaaaccgcc ataacagcaa
     1801 caccgcacag cgtggcgata accgctttgt gcgggtaacg tgggacgaag ccctcgacct
     1861 gttctaccaa gagttagaac gggtacaaaa agagtacggc ccgtgggcgt tacacaccgc
     1921 caatgtgggc tggcgttcaa ccggtcaatt ccacagttgc ggtaaccata tgatccgagc
     1981 gattgcgatg cacggtaaca gtgtcggcac ggcaggcgac tattctaccg gtgcagggca
     2041 gaccattctg ccgtatgtgc tgggttcaac cgaagtctat tcgcagggca cgtcttggga
     2101 aatcatctta aaagagagcg aaaacatcat tttctgggca agcgacccgg tgaagaacct
     2161 gcaagtgggt tggaactgcg aaacccacga agcctatgcc tacttggaaa aactgaaaga
     2221 gaaagtggca gcaaaaggcg tgaatgtgat ctgtgttgat ccggtgaaaa gcaaaaccca
     2281 gaatttctta ggttgcgaac agcagtacat caatccacaa accgatgtgc cgtttatgtt
     2341 ggcacttgcc cacacgctct acaccgagaa cctgtacgac aaaaaattct tggatatgta
     2401 cacggtcggc tttgaaaaat tcctgccgta tctgttaggc gaaaccgaag acaaagtggc
     2461 gaaaaccccg gaatgggcgg ctgaaatttg tggtatttcc gccgacaaaa tccgtgaatt
     2521 tgcccgaatg cttgccggca aacgcaccca atttattttc ggctgggcaa ttcaacgcca
     2581 acaacacggc gaacaaccgt actggatggg agcggtgcta gcctcaatgt tagggcaaat
     2641 cggcttggaa ggcggcggca tcagctatgc tcaccattac agctctatcg gtattcctga
     2701 atccggtgcg gcaatgccgg gggcgttccc gctcaatatt gatgaggggc aaaccccgaa
     2761 atacgacaac aaagattaca aaggctacag cgacaccatt ccggtggctc gtatgaccga
     2821 cagcttgctc cacgccggcg aaaccattga ttacaacggc aaaaaagtga cttacgctcc
     2881 gttcaaaatg gcgattttca ccggctgtaa ccaatggcac cgccattccg agcggaacaa
     2941 aatgaaacag gcgttccaaa aactggaaac cattgtttcg atcaactaca gttggacggc
     3001 aacctgccgc ttctccgaca tcgtgctgcc ggcctgtacc ccatttgaac gcaacgatat
     3061 tgacgcctac ggctcataca gcaaccgtgg cgtgattgca atgcacaagc tggttgatcc
     3121 actctacgac tctcgctccg actttgagat tttccgagat ctctgccgcc gtttcggtaa
     3181 agagaaagag tacagccgtg gaatggacga aatgcagtgg gtagaaaaac tctacaaaga
     3241 ttgtcgccgt gaaaacagag gtaaatttga aatgccggag tttgctgaat tttggcaaaa
     3301 aggctatgtg ctgttcccag agggcaaacc ttgggttcgc cacgccgatt tccgtgaaga
     3361 tccggaattg cacgcacttg gcacaccgtc cggctttatc gaaatttaca gcaacaaaat
     3421 cgcctctttc ggctatgacg attgcaaagg tcacccaatg tggtttgaga aagccgaacg
     3481 ttcacacggc ggtaaaaatt ccgacaaatt cccgtactgg ttgcaatcag ttcacccgga
     3541 caaacgctta cactctcaac tttgtgaatc gaaagaactg cgtgaaactt acagcgtgca
     3601 agggcgtgaa ccgctttaca ttaacccaca agatgccgcc aaacacggca ttcaagacgg
     3661 agatttagtg cgagtgttta acgaccgtgg gcaagcgatt gtgggggcgg tgctttccga
     3721 caacttcccg accggcgtag tgcgtttaca agaaggggct tggtattcac cccttgacga
     3781 aagcgtgggg gcgattgaca cctatggcga cccgaacaca atgaccctcg acatcggcac
     3841 gtccaagttg gcacaagcgg tttccgccaa cacttgcttg gtgaacatcg agaaatttat
     3901 cggcaatgcc ccggctccga atggctttaa cggtgcgatt gaggtaacgg cttaatgacg
     3961 ttggcaatca gtcgtgaaga acggcaattt atctatggct ggtttaacgc aatgctcgcc
     4021 cgagaacttt cggacgagca gttaaatgcc ttacaagcgg gcgaatttga cgattttttt
     4081 gcatacctca gcgaattggg ttttgccgaa caggttgcaa aatttcaagc agaagtgacc
     4141 gcttgtaggc agctggaatt tcctcgtcta gaactggcgg ctgactttgc ccaactgttt
     4201 ttattggaag gcaatctcag tgccattcct tacgcctccg cctatttaag cggcgaagcg
     4261 ttagcgaaaa atttggcgga aatggacaga ctgctgggcg aatttgggct gcaagttaat
     4321 cgagaaacca aagagccgag cgatcacctt tgcgtttatc tcaccctttt tgaccgcttg
     4381 cttgctgaaa aaccgttgga aacgcaacag caatttctgc aaaaccaact gatgagctgg
     4441 ctccctgcgt ggttcgccaa agccaaaacc gtcaaaacta acaccggcgt ttatccggcg
     4501 ttgctaaggt tgtttgaaca ggtgttgctg aaggaacaaa gataaagtta tttatttgag
     4561 aatgatttgt attgagcgaa ttgagaaagg aagttaaaat ttcaacttcc ttttttattg
     4621 gctggattta tccacattat tacaagcggt tatttttata aaaaaattta caaaggagac
     4681 attaatgcaa aataataaaa ccacctacct aaattggtac gatttatgcg tgctaaccct
     4741 gatcctgttt ggtgaggcaa tttatacctc aacccagcaa tatttcatca ccaccgaaat
     4801 gcaaggtaat tttgaagaaa atttaacctt ttcaaatgaa caaaattatt gggcattact
     4861 gacgcaactt gcattgttgc tgattgcaat gggctatcta aaatggcgga attttgattt
     4921 cagccgttgg gtgattcgtc cctcttggct ggcttttttt agcggtatcg ggttgtttat
     4981 cttgctctcg ttagcgatgg atctctattt tgaaacatta tatggtgttt acaacctagt
     5041 gtatccggag ccattttggg cagtattgag agaagtgaat atttctaccg tgctgtatgc
     5101 gattttaaac ggcttttatg aagaaatctt tttcctcggt atttgcttgg cggttaaacc
     5161 tgaatattta aaatgggcgg tgatttattc actgattatt cgggtgagtt ttcataccta
     5221 tcaaggaatg gaaagtgcgt taggattagg aatttggctc ggtttaatta tttatttgct
     5281 ctactcacgc tcaaagcaga aaaacctatt tccattttgg gtggcacaca gtattgcgga
     5341 tattttgggg ctgagtttgt tggcttattt ttagttaaag gtgctacatt taagcggcat
     5401 aagttgatag agctgatcgt accagtagca taaaaacatt attatttgta tggaaaggtg
     5461 ctt
//
LOCUS       DANLXS010000163         5430 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.164, whole genome shotgun sequence.
ACCESSION   DANLXS010000163 DANLXS010000000
VERSION     DANLXS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.164"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..2063
                     /gene="glyS"
                     /locus_tag="PXD71_002169"
     CDS             <1..2063
                     /gene="glyS"
                     /locus_tag="PXD71_002169"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL6292244.1"
                     /translation="TQNFLAEIGTEELPPKALKKLATAFAENVENELNQAGLSFEKVE
                     WFAAPRRLAVKVLSLAESQPSKEVEKRGPAVSAAFDAEGKPTKAAEGWAKGCGITVEQ
                     AERIATDKGEWLVHRAVIEGQPTKNLLVDIIAKSLANLPIPKTMRWGDKTEQFVRPVH
                     TVTLLFGAELIEGEILGVKSGRTIRGHRFLGEREFQIENADQYPQLLKDRGSVVADFN
                     ERKALILAKSQEKATALGGVADIEEDLLDEVTSLVEYPNVLTAKFEERFLAVPAEALV
                     YTMKGDQKYFPIYSKDGKLLPHFIFVSNINPEDPSKIIEGNEKVVRPRLTDAEFFFKT
                     DLKQKLEDQLPRLETVLFQQQLGTLRDKTARIEALAGEIAKQIGADETKAKRAGLLSK
                     CDLMTNMVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDNLPNSLVACSV
                     ALADKFDTLTGIFGIGQAPKGSADPFALRRAALGSLRIIVEKNLPLDLTDLVAKSAQL
                     FGDKLTNKDVVEDVVDFMLGRFRAWYESEGIAVDVIQAVLARRPTKPADFDARVRAVS
                     HFRTLDSAEALAAANKRVSNILAKADIAIGDIDVSACVEPAEKALAEAVIALKAEVQP
                     LIAQGDYTAVLDKLANLRQPVDAFFDGVMVNAEDQKLRQNRLAILSTLQGLFLQVADI
                     SLLQ"
     gene            complement(2168..3469)
                     /locus_tag="PXD71_002170"
     CDS             complement(2168..3469)
                     /locus_tag="PXD71_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDL6292245.1"
                     /translation="MNIHCEKLVQSILHAVNHEVVPALGCTEPISLALASAIARQYLG
                     HLPERIEAKVSPNLMKNGMGVAVPGTATVGLPMAAAIGAIGGEPDGGLEVLKNITPEQ
                     VKQAKAMLNTDKVSVSIFETEHILYSEATLFHQANRVCVRIAEHHTNVIYIEKNGQTI
                     LSKPCAVNNDNTTEIFASLSAKDIFNFSMQIELEKIRFIEQAAQLNSALSQEGLRADY
                     GLHIGRTLQKQIGLGLISDDLLNRIVIETTSASDARMGGASLPAMSNSGSGNQGITAT
                     MPIVVVARHLNVTDEQRIRALFLSHLMAIYIHSKLPKLSALCAVTTAAMGSCAGIAWL
                     LTGKFENISMAISSMIGDISGIICDGASNSCAMKVSTSVTSSYKSILMSMDNSQVTGN
                     EGIVEHDIDRSINNLCSIASRSMQYTDRQVIEIMVNKPKLK"
     gene            complement(3483..4760)
                     /locus_tag="PXD71_002171"
     CDS             complement(3483..4760)
                     /locus_tag="PXD71_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292246.1"
                     /translation="MDTQKLSPAEFKQATKFDSTDFGWIIMSIGMAIGAGIVFLPVQV
                     GLMGMWVFLLSSIIGYPAMYLFQRLFINTLAESPECKDYPTVISGYLGKNWGIFLGVL
                     YFIMLVIWMFVYSTAITNDSASYLLTFGITNTLLSDNPFYGLVLICILVALSSRGEKL
                     LFKISTFMVLTKLFVVAALGLAMIGMWQLHNIGAMPPFGKLIKDAIITLPFTLTSILF
                     LQTLSPMVISYRAKEQSREVARYKALRAMNIAFAILFITVFFYAVSFTLAMGREEALK
                     AYEQNVSALAIAAQFFPGSWATYVGVALNIFAVMTAFFGVYLGFREATQGIVMNLLLR
                     KFPAEKINPNYVQKGIMLFSILLAWSAIVLNAPVLSFTSICSPIFGLVGCLIPAYLVY
                     KVPALFKYKGLALNIIIITGILLCISPFLTFLE"
     gene            complement(4789..>5430)
                     /locus_tag="PXD71_002172"
     CDS             complement(4789..>5430)
                     /locus_tag="PXD71_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL6292247.1"
                     /translation="DLDKEIMLSYFSLSEAIAGLLGEFCEVVIHSLNAYEKSVIKIIN
                     GHHTNRKIGSPITDKGLQLLKCYQKDQSNTELVYFSRLNNGNLVKSTTHIIIGHEGKP
                     IGLFCINLNLSTPLDKTVSSFMNYDPIPPHYKSENFITHPKELIEETFLKAVTHIEQH
                     TTKHSKLYKKSIIFYLFENGIFELKEAVPIVAEKLSLTSHAIYKHLREFKNNT"
BASE COUNT         1601 a         1099 c         1234 g         1496 t
ORIGIN      
        1 caacacaaaa cttccttgcc gagatcggca cagaagagct gccaccgaag gcactcaaaa
       61 aattagcgac cgcatttgcg gagaatgtag aaaacgaatt aaaccaagca ggtctttcgt
      121 ttgagaaagt ggaatggttt gctgctcctc gccgtttggc ggtgaaggtg ttgagccttg
      181 cggaaagcca gccgagtaaa gaggttgaga aacgagggcc ggcggtgtcg gcagcgtttg
      241 atgccgaagg caagccgact aaggcagcgg aaggctgggc gaagggctgc gggattaccg
      301 tagagcaagc tgagcgtatt gccaccgaca aaggcgaatg gttggtgcat cgtgccgtga
      361 ttgaaggtca gccgactaaa aatctgttgg tcgatattat cgccaaatcg ctggcgaact
      421 tgccgattcc aaaaacgatg cgttggggcg ataaaaccga gcagttcgtc cgccctgtgc
      481 ataccgtgac acttttattc ggggcggagt tgatcgaagg tgagatctta ggcgtgaaaa
      541 gcggccgtac tattcgtggt caccgtttct taggtgaacg tgagttccaa attgagaacg
      601 ccgatcaata tccgcaactg ttgaaagatc gtggatctgt ggtagcagat ttcaatgagc
      661 gtaaagcctt aattcttgca aaatcacagg aaaaagcaac cgctttgggt ggcgttgccg
      721 atattgaaga agatttgctg gacgaagtga cctcgttggt ggaatacccg aacgtactca
      781 ccgccaagtt tgaagagcgt ttccttgctg tgccggcgga agccttagtt tacaccatga
      841 aaggcgacca aaaatacttc ccgatttatt caaaagacgg caaattgtta ccgcacttta
      901 tttttgtgtc gaacatcaat ccggaagacc caagcaaaat catcgagggt aacgaaaaag
      961 tggttcgccc acgtttgacc gatgcggagt tcttcttcaa aaccgactta aaacagaagt
     1021 tggaagacca actgccacgc cttgaaaccg tgttgttcca gcaacagctt ggcacattgc
     1081 gtgataaaac cgctcgcatt gaagcgttgg caggtgaaat tgcgaagcaa atcggggcag
     1141 atgaaaccaa agcgaaacgt gcaggtctcc tctcaaaatg tgacctgatg accaatatgg
     1201 tgtttgaatt tactgatacc caaggcgtga tgggtatgca ctatgcccgc cacgacggtg
     1261 aagatgaaga agtggcggtg gcgttaaacg agcagtatat gccacgtttt gctggcgata
     1321 acttgccaaa ttcattggtg gcttgctcgg tggcgttggc ggacaaattt gacacgctaa
     1381 cagggatttt cggcattggt caagccccga aaggcagtgc cgacccgttc gccctacgcc
     1441 gtgcggcact tggctcgttg cggattattg tggagaaaaa tttaccgctt gatttaaccg
     1501 atttagtggc gaaatctgct caattatttg gcgataaact caccaacaaa gacgtggttg
     1561 aggacgtggt ggactttatg ctcggccgct tccgtgcgtg gtatgagagc gaaggcattg
     1621 cggtggacgt gatccaagcc gttctcgccc gccgtccgac caagcctgcc gacttcgatg
     1681 cccgtgtgcg tgccgtgtct cacttccgca cccttgacag tgccgaagcc ttagccgcag
     1741 cgaataaacg ggtgagtaac attcttgcca aagccgatat tgcaattggc gacattgatg
     1801 tttcagcttg cgtagagcca gcagaaaaag cattagccga ggcggttatt gcgttgaaag
     1861 ccgaagttca accgctgatt gcacagggcg actacaccgc tgtgctggat aaattagcga
     1921 acctccgcca accggttgat gccttctttg acggagtaat ggtaaatgca gaagaccaaa
     1981 aactgcgtca aaaccgcctc gccattttaa gcacgctaca aggcttgttc ctgcaagtgg
     2041 cggatatttc gctgttgcaa taacaataag tttgcaataa ttgcaaaatt ttaatgaaaa
     2101 tagaccgctt gcagtaattg tgatctgcaa gcggtctttt ttgcttgaaa ttttaccaaa
     2161 ggcgggatta tttaagtttt ggtttattca ccataatctc gatgacttgg cggtctgtgt
     2221 attgcatact acgagaggca atgctgcata agttattaat tgaacgatca atatcgtgtt
     2281 cgacaattcc ttcgttgcct gttacttggc tattatccat agacatcaga atggatttat
     2341 agcttgatgt tacgctggtg gagactttca tcgcacaact gtttgaagca ccatcacaga
     2401 taataccgct aatatcgccg atcatactac taattgccat actgatattc tcaaatttcc
     2461 ctgtgagcag ccacgctatg ccggcacagc ttcccatagc cgcggttgtt acggcacaga
     2521 gtgcagataa tttcggtaac ttgctgtgaa tataaattgc catgaggtgt gataaaaaga
     2581 gtgctctaat gcgttgctca tcagtgacat ttaaatggcg ggcaacgaca acaatcggca
     2641 tagttgctgt aattccttga ttacctgaac ccgaattgct catagcaggt aaacttgcac
     2701 cgcccattct agcatcagag gcagaggtgg tttctataac aatgcgatta agcaaatcat
     2761 cactgattag acctagacca atctgttttt gcaatgtcct tccgatatgt agcccataat
     2821 cggcacgtaa tccttcttgt gagagtgcgg aatttaattg tgctgcttgc tcaataaagc
     2881 gaattttttc caattcgatt tgcatagaaa aattaaatat atcttttgcc gaaagtgaag
     2941 caaagatctc agtagtatta tcgttattga cggcacaagg tttgcttaaa atagtttgcc
     3001 cgtttttttc aatgtaaatg acattggtat ggtgttcagc aatacgaaca catactcgat
     3061 tcgcttggtg aaatagtgtt gcttcagaat atagaatatg ttctgtttcg aaaatagaga
     3121 cagacacttt atcggtattt aacatagctt tagcttgctt aacctgttct ggcgtaatat
     3181 tttttaatac ttctaatcca ccatcaggct cccctccgat tgctccaata gctgccgcca
     3241 taggcaatcc gacggttgca gttcccggta ctgctactcc catcccattt ttcatcaagt
     3301 taggggaaac ttttgcctca atacgttctg gtaaatgacc taaatattgg cgtgcaatgg
     3361 cggatgcaag ggcgagagaa ataggttctg tacagcctaa tgcagggaca acttcatggt
     3421 tgactgcatg taaaatggat tgtactaatt tttcgcagtg aatattcata gataaaacct
     3481 tattactcta aaaatgtgag gaatggagaa atacataata aaatgccggt aatgataata
     3541 atattgagtg ctagcccttt atatttgaat aatgcaggca ctttatagac taaataggcc
     3601 ggaatcaagc acccaactaa gccaaaaata ggactacaga ttgaggtaaa gcttaaaacc
     3661 ggtgcattta atacaatcgc actccaagcc agcagaatgg aaaataacat aatccctttc
     3721 tgcacataat taggattgat tttttcagcc gggaattttc ttaataatag gttcatcaca
     3781 atgccttgag tggcttctct aaaacctaaa tataccccga aaaaggctgt cataacggca
     3841 aaaatattta atgcaacacc tacataggtt gcccaactac cagggaaaaa ttgggctgca
     3901 attgctaatg cggaaacatt ttgctcgtat gctttgagcg cttcttctct ccccattgca
     3961 agtgtaaaag agacagcgta aaaaaaaacg gtaataaata agatagcaaa tgcaatattc
     4021 atcgctcgta gggctttata gcgcgcaact tcacgagact gttctttagc acggtaagaa
     4081 attaccattg ggctgagagt ttgcaagaag agtatagagg ttagggtgaa aggaagggta
     4141 atgatggcat cttttataag tttgccgaaa ggaggcattg caccgatatt atggagttgc
     4201 cacattccaa tcattgcaag ccctaaggcg gcaacgacaa atagcttagt taataccata
     4261 aatgtagata ttttaaacag tagcttttcg cctcgagaag ataaggcaac taatatacag
     4321 attagcacta agccataaaa tggattatca gagagtagtg tattggtaat gccgaaggta
     4381 agaagatagg aggcactgtc gtttgtaatt gcggtagaat aaacaaacat ccaaatcact
     4441 aacataataa aataaagtac acctaaaaag attccccagt ttttgcccag gtagccacta
     4501 ataacggtgg ggtaatcttt gcattccgga gattctgcta aagtattaat gaacagacgc
     4561 tgaaaaaggt acatcgccgg gtatccgata atagaagaaa gtagaaaaac ccacatgccc
     4621 ataagtccta cttgtaccgg taaaaagacg atgcctgccc cgatagccat accaatactc
     4681 ataatgatcc agccgaagtc ggtagaatca aattttgttg cttgtttaaa ttcggcaggc
     4741 gagagttttt gagtatccat agagtctcct taaatcgtaa cagtataatt aagtgttatt
     4801 tttgaattct cttaaatgtt tataaattgc gtggctggtt agggagagtt tttctgccac
     4861 aataggtact gcctctttta gctcaaagat gccattttca aacaaataga agataatgga
     4921 tttcttatag agttttgaat gtttggtagt gtgttgctca atgtgagtaa ctgcttttag
     4981 aaaggtttct tcaataagtt cttttgggtg agtaataaag ttttcacttt tataatgagg
     5041 cggaatagga tcgtaattca taaaagagct aacggtttta tctaatggtg tcgataaatt
     5101 taaattaata caaaataatc cgataggttt gccctcatgc ccgataataa tatgcgtggt
     5161 ggacttaact aagttaccgt tatttaaccg tgaaaagtaa accagctcag tattagattg
     5221 atctttttgg taacatttta gtaactgtaa tcctttatct gtaataggcg aacctatttt
     5281 tcggttagta tgatgtccgt taatgatttt aataacggat ttttcatagg cattcaatga
     5341 atgaataacc acctcacaga attctcctaa taaccctgct attgcttcac ttaatgagaa
     5401 ataagacagc ataatttctt tgtctaaatc
//
LOCUS       DANLXS010000164         5419 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.165, whole genome shotgun sequence.
ACCESSION   DANLXS010000164 DANLXS010000000
VERSION     DANLXS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.165"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            43..2610
                     /gene="clpB"
                     /locus_tag="PXD71_002173"
     CDS             43..2610
                     /gene="clpB"
                     /locus_tag="PXD71_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL6292248.1"
                     /translation="MNFEKMTTKLQEALAESQSLAVGKDNPYIEPAHLLYALLKQEGG
                     SIASLFTTLNVDVPTLIRELQQILDRLPKVQGGNTQVSQQLVRLLNQSDKLAQQFGDS
                     FISSELFVLAALDDNGDLGKLFKQFGLNKEKLTQAISQIRGGDTVNNQNAEDTRQALK
                     KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPEGLKNKRVLSLDMGALIAGAKYRGEFEERLKAVLNDLAKEEGQVILFID
                     EIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQYIEKDAALERRFQKV
                     LVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYVSDRQLPDKAIDLI
                     DEAASSLRMEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEEMAAKE
                     REYSELEEIWKAEKSALLGTQHIKSELENARIAMDQARRENDFEKMSELQYGVIPNLE
                     KQLQAVENGEKNADAQLLRTKVTEEEIAEVLSKATGIPVAKMMEGEKEKLLRMEEVLH
                     ARVIGQNEAVDAVANAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELSKTLANFLFDD
                     PDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRHPYSVVLLDEVEKAH
                     PDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHLIRENAYKGYDVVKEMVM
                     EVVGQHFRPEFINRIDETVMFHSLGQEHIRSIAKIQLQRLIQRLAERGYEVTVTDAAL
                     DHIGKAGFDPLFGARPLKRAIQQELENPLAQQILSGKLLPNKAVTVDYQNGQIVATQ"
     gene            2824..3966
                     /locus_tag="PXD71_002174"
     CDS             2824..3966
                     /locus_tag="PXD71_002174"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL6292249.1"
                     /translation="MSQLFPEATIRTQAPYRNDIVGSFLRTDALKKAREDFNQQKITR
                     DELREVTRQEVKKLVELQKQHGVKAVSDGEFSRTWWHLDFLAELDGMDWQETEVFNVN
                     FTGHKPKGQTVKIVGDIDFSESHPFVDAYRLLKEAAGDYPTKFTIPAPTMLHIICCVR
                     AEDYQPLPQYQDEQKLYDDIANAYIKAMHKFYDMGLRNLQLDDTSWGQFCAEDKRKEF
                     AARGFNLTDLAEKYVALLNRIVDAKPADMSITMHICRGNFRSSWFSEGGYGPVAEVLF
                     GKCRVDGFFLEYDTERAGTFEPLRHIKDQQVVLGLITSKFGDLEDKNVIIERIKEAAQ
                     IVPINQLCLSPQCGFASTEEGNILTEAAQWAKLNLIKEIAEEVWGS"
     gene            complement(4023..5414)
                     /locus_tag="PXD71_002175"
     CDS             complement(4023..5414)
                     /locus_tag="PXD71_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDL6292250.1"
                     /translation="MENTPLIEPSKNTRNSLIFVADAILFIILLNTLPFTPEANKGLA
                     LLIFIAVLWLTEALHVTVTALLIPILAVALGLVKSKEALVAFADPTIFLFFGGFALAT
                     ALHIQQLDRMIANKIMALAKGKLSIAVIYLFAVTAFLSMWMSNTATAAMMLPLAMGIL
                     SKLDQKQNHNTYLFVLLGIAYSASIGGMGTLVGSPPNAIVASQLNLTFADWLKYGLPI
                     MLILFPLMIATLYIVFKPNFSVSFDRSFEKIELNRSRIITLAIFVFIALGWIFGDKIN
                     PIISAFLGINGKIASFDTILALIAAALICITRVANWQQIQENTEWGVLFLFGGGLTLS
                     AVLGQTGASKIMADGVVSLIEGGHFYLIGLIVAAFIIFLTEVTSNTASAALLVPIFIS
                     IAQALNMPPVGLALIIGLGASCAFMLPVATPPNAIVFGTGQIKQSEMIKVGFWLNIIS
                     IFVIATVGYLFWL"
BASE COUNT         1771 a         1104 c         1167 g         1377 t
ORIGIN      
        1 ataaaaattt acactttcta aactcattgt aaggagaaag aaatgaattt tgaaaaaatg
       61 accacaaaac tgcaagaagc cttggctgaa tcacagtcgc ttgcggtagg aaaagataat
      121 ccgtacatcg aaccggcaca tttgctctat gcgttgttaa aacaagaagg tggctcgatt
      181 gcttctttgt ttaccacatt aaatgttgat gtaccgactt taattcgtga attacaacaa
      241 attttagacc gcttgccgaa agttcaaggc ggtaacaccc aagtttcaca acagttggtc
      301 agactgttaa atcaatcaga caaactggca caacaattcg gcgacagctt tatttcatca
      361 gaactatttg ttctagctgc acttgatgac aatggcgatt tggggaaact atttaaacaa
      421 tttggcttaa acaaggaaaa actaacacaa gccatcagcc aaattcgagg aggagacaca
      481 gtgaataatc aaaatgcgga agatacccgc caagcactca aaaaatatac gattgattta
      541 accgaaagag ccaaagccgg taaattagac ccggttatcg ggcgtgatga agaaattcgc
      601 cgtgcggtgc aagtcttaca acgccgtacc aaaaacaacc ctgtgttaat tggtgaaccg
      661 ggtgtgggga aaacggcgat tgtagaaggc ttggcacagc gtattgtaaa cggcgaagtg
      721 ccggaaggct tgaaaaataa gcgtgtactt tcgctggata tgggagcatt aattgccggt
      781 gcgaaatatc gtggtgaatt tgaagagcgt ttaaaagcgg ttttaaacga cctggcgaaa
      841 gaagaagggc aagttatcct ctttattgat gaaattcaca cgatggttgg agcaggcaaa
      901 acagacggag caatggacgc cggcaactta ttaaaaccaa gccttgcccg tggtgaattg
      961 cactgtgtgg gggctacaac ccttgatgaa tatcgccaat acattgaaaa agacgcagcc
     1021 cttgaacgcc gtttccaaaa agtattggtg gacgaaccaa cggttgaaga tacgattgcg
     1081 attttacgtg gtttgaaaga gcgttatgaa atccatcatc acgttcaaat tacagaccct
     1141 gccatcgtgg cggcggcaac gctttcgcac cgttatgtgt ctgaccgtca attgccggat
     1201 aaagcgattg acttgattga tgaagccgct tcaagcctgc gtatggagat tgattctaaa
     1261 cctgagccgt tggataaatt agaacgccgc attatccaac tcaaattgga acgccaagcg
     1321 ttacaaaaag aggaagatga agcgagccgt cagcgtttag ccaaattaga cgaagaaatg
     1381 gcagcgaaag agcgtgaata ttccgagctg gaagaaatct ggaaagcgga aaaatccgcc
     1441 ttacttggta cacagcatat taaatcggag ttggaaaatg cccgaattgc gatggatcag
     1501 gctcgccgtg aaaacgattt cgagaagatg tcggaattgc aatatggcgt gattccaaac
     1561 ttggaaaaac agttacaagc ggtcgaaaat ggcgaaaaaa atgcagatgc tcagctcctc
     1621 cgtactaaag tcaccgaaga ggaaattgcg gaagtgcttt caaaagcaac gggcattcct
     1681 gttgcaaaaa tgatggaagg cgagaaggaa aaactccttc gtatggaaga agtattacac
     1741 gctcgggtta tcgggcaaaa tgaagccgtt gatgcggtgg caaatgcgat tcgccgtagc
     1801 cgtgccggac tttccgatcc gaataaaccg attggttcat tcctattctt aggaccgacc
     1861 ggcgtgggta aaaccgagtt aagcaaaacc ttagctaatt tcttgtttga cgatcctgat
     1921 gctatggtgc gaattgatat gtctgaattt atggaaaaac acagcgtatc ccgcttagtg
     1981 ggcgcacctc cgggctatgt cggctacgaa gaaggcggct atttgaccga agcggttcgc
     2041 cgccatccat attcggtagt gttacttgat gaagtggaaa aagctcaccc tgatgtgttt
     2101 aacattttat tgcaagtgtt agatgacggg cgtttaaccg acgggcaagg cagaacagtc
     2161 gatttccgca atacggtagt gattatgacc tcaaacttag gctcgcattt aattcgagaa
     2221 aatgcgtata aaggctatga tgtggttaaa gaaatggtga tggaagtcgt cggacagcac
     2281 ttccgccctg aatttatcaa ccgtattgat gaaacggtga tgttccactc acttggtcaa
     2341 gagcatatcc gttcgattgc gaagattcag ctacaacgct taatccaacg cttggcagaa
     2401 cgaggctatg aggtgacagt aaccgatgca gcattagatc atatcggcaa ggctggtttc
     2461 gatccgctgt tcggagcaag accactgaaa cgtgcgattc aacaagagct tgaaaatccg
     2521 cttgctcaac agatcttaag cggtaaatta ttaccgaata aagccgttac ggtggattac
     2581 caaaacgggc agattgttgc aacacaataa tgtctccgat ggtgcgagcg tcctcgctcg
     2641 taccttacaa gcggtggatt tatcgtaaaa atttgcaaaa aattgctcga aaataaccgc
     2701 ttgcacagta aaaattttca tattgccctt cattaatttt cataagctaa tgaagggctt
     2761 tttattactc tttttgctat cattaacggc gtttataacc aatacaatta caaggagtaa
     2821 tttatgagcc aattatttcc ggaagcaaca atccgcactc aagccccgta tcgtaatgat
     2881 attgtaggca gctttctacg taccgatgcg ttaaaaaaag ccagagaaga tttcaatcag
     2941 caaaaaatca cccgtgacga attgcgtgaa gtcacacgcc aagaggtcaa aaaattagtt
     3001 gagttgcaaa agcaacacgg tgtaaaagca gtctctgacg gtgaatttag ccggacttgg
     3061 tggcatttag atttcttagc tgaacttgat ggaatggatt ggcaagaaac cgaagtcttt
     3121 aacgtgaact ttaccggtca taaacccaaa gggcaaacgg taaaaatcgt gggcgatatt
     3181 gatttctccg aaagccaccc ttttgtggac gcttatcgtc ttttaaaaga agctgccggc
     3241 gattacccaa ccaaattcac gattccggca ccgacaatgt tacatattat ctgctgtgtg
     3301 cgtgcggaag attaccaacc gttaccacaa tatcaagacg aacaaaaact gtacgatgat
     3361 attgctaatg cttatatcaa agcaatgcac aaattctacg atatggggct acgtaactta
     3421 caattagacg acaccagttg gggacaattc tgtgccgaag ataaacggaa agagtttgca
     3481 gccagaggat ttaatttaac tgatttagcg gaaaaatatg tcgcattatt aaaccgtatt
     3541 gttgatgcaa aaccggcgga tatgtcaatt acgatgcaca tctgccgtgg taacttccgt
     3601 tcttcttggt tctcggaagg cggttatgga ccggttgccg aagtattatt cggtaaatgc
     3661 cgtgttgacg gcttcttctt agaatacgat accgaacgtg caggcacttt cgagccacta
     3721 cgccacatta aagatcaaca agtggtacta ggcttaatta cctctaaatt tggcgattta
     3781 gaagataaaa atgtgatcat tgagcgtatt aaagaagcgg ctcaaattgt gccaatcaat
     3841 cagctatgct tatcaccaca atgcggcttt gcctccaccg aagaaggcaa tattttaacc
     3901 gaagccgcac aatgggcgaa actaaactta attaaagaaa ttgcggaaga agtttggggt
     3961 agctaatcct ctataataca aatagccgag taaatactcg gctattttag tttgttttaa
     4021 cattacaacc agaagagata cccaacagtc gcaatcacaa agatagagat aatatttagc
     4081 cagaaaccaa ctttaatcat ctcactttgt ttaatttgcc ctgtaccaaa tacaatcgca
     4141 ttcggtgggg tggcaaccgg caacataaag gcgcaagatg ctcctagacc aataattaag
     4201 gctaatccga ccggtggcat atttaatgct tgtgctatag agatgaaaat cggcactaac
     4261 aaagccgcac tggctgtgtt agaggtaacc tcagttagga aaataataaa tgctgccaca
     4321 attaaaccaa ttaggtaaaa atgcccccct tcaattagtg agacgacccc atcagccata
     4381 attttgctcg caccagtttg tcctaataca gcacttaacg tcaaaccacc accaaatagg
     4441 aataataccc cccactcggt gttttcttga atctgttgcc aatttgcgac acgagtaatg
     4501 caaataagtg ctgcggcaat caatgcaaga atggtgtcaa agctcgcaat tttgccatta
     4561 attcccagaa atgcagaaat aatagggtta attttatcac caaaaatcca accaagcgca
     4621 ataaagacga aaatagccaa ggtgataata cgagaacgat ttaattcaat tttctcaaat
     4681 gatcgatcaa aactaactga aaaatttggt ttaaatacga tatatagtgt tgcaatcatt
     4741 aatgggaata aaattagcat aattggcaaa ccatatttta accaatcagc aaaagtcaga
     4801 ttcaattgag aagcaacaat cgcattcggt gggcttccca ctaaagtacc cattccacca
     4861 atactggcac tataagcaat tcctaataac acgaaaagat aagtattatg gttttgtttc
     4921 tgatcaagtt tacttaaaat ccccattgcc aaaggtaaca tcattgcagc tgtagctgta
     4981 ttactcatcc acattgacaa aaacgctgtt accgcaaata aataaataac tgcaatcgat
     5041 aatttacctt tagctaatgc cataatttta ttggcaatca tacgatctag ctgctgaatg
     5101 tgtaatgctg tcgctaaggc aaagcctcca aaaaataaga aaatcgttgg atcagcaaaa
     5161 gcaacaagag cttcttttga tttaaccagc cccagtgcta cagccaaaat cggaattaat
     5221 aatgccgtaa ccgtaacgtg taatgcttct gttagccata agacggcaat gaaaattaac
     5281 aatgccaacc ccttattggc ttcaggggta aaaggcaagg tatttaacaa aataataaat
     5341 aaaatagcat ctgcaacaaa aatcaagcta ttgcgtgtat ttttactcgg ttcaataaga
     5401 ggagtatttt ccataaaaa
//
LOCUS       DANLXS010000165         5397 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.166, whole genome shotgun sequence.
ACCESSION   DANLXS010000165 DANLXS010000000
VERSION     DANLXS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.166"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            1..606
                     /locus_tag="PXD71_002176"
     CDS             1..606
                     /locus_tag="PXD71_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDL6292251.1"
                     /translation="MHFAGLISPGPDFFYVVRQSASHSVKSGVLAATGISIGIIFWAA
                     FAIFGLAWLSNSIGSLFQYAIMIIGGCYLVYIGSQMVRVTENIIFVENKAQLTPLNPW
                     QEIKKGLLINIFNAKAGVYFTSVISAYVDNLSNTLQMFELLLLFVVSTFIYFCVVALL
                     FSRRPVRLFYAKYSRYIDNISGVIFILFGSMLIYEGFSHFI"
     gene            718..1323
                     /gene="ruvA"
                     /locus_tag="PXD71_002177"
     CDS             718..1323
                     /gene="ruvA"
                     /locus_tag="PXD71_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL6292252.1"
                     /translation="MIGRLHGKIIEKQPPEILLDVQGVGYELLLPMTSFYSLPQIGEE
                     ATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFATAI
                     ENEELAKLTKIPGIGRKTAERLLVELKGKFKGMAQSDFFVEVSHEQIVASNTPDPANE
                     ALDALIALGYKPADAEKMIKKVNKSGATSEQLIREALKNSL"
     gene            1460..2464
                     /gene="ruvB"
                     /locus_tag="PXD71_002178"
     CDS             1460..2464
                     /gene="ruvB"
                     /locus_tag="PXD71_002178"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL6292253.1"
                     /translation="MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVHDQMEIF
                     IKAAKLREEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLT
                     NLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTLIVKRSADCLNLNLSADGAYEVARRSRG
                     TPRIANRLLRRVRDYADVRNNGIITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERF
                     DGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGITQID
                     "
     gene            complement(2563..3246)
                     /gene="artM"
                     /locus_tag="PXD71_002179"
     CDS             complement(2563..3246)
                     /gene="artM"
                     /locus_tag="PXD71_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDL6292254.1"
                     /translation="MWWDYLVEISQGIGTSLQLTFVALIIAFILAIILTFILSLENRP
                     LKWLVNGFITLFTGTPLLIQFFLIYSGPGQFEWITESAAWVLLSDAWFCAMLALALNS
                     AAYSTLLFHGAVKAIPKGQWETCAALGLSRLDTLKILVPYALKRALPSYSNEIVLVFK
                     GTSLASTITIMDIMGYARQLYGTEYDALSIYGMAGVIYLVITGIMTIVLRKLESRVLA
                     FERFETEKA"
     gene            complement(3246..3917)
                     /gene="artQ"
                     /locus_tag="PXD71_002180"
     CDS             complement(3246..3917)
                     /gene="artQ"
                     /locus_tag="PXD71_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDL6292255.1"
                     /translation="MSSDILNLLVNATLMTLGIALASLLIGMLLSIFFVVLETSKIAC
                     IRKPTSIIITLLRGLPEILVVFLIYFGLIQVIFLITDEFAELNAFWCGAIALGIIFAA
                     YASQSLRGAIKAIPVGQWESGTALGLSRAQVFVRIILPQVWRHALPGLSNQWLVLLKD
                     TALVSLIGVDDLMRQSELINANTHQPFTWYGIAALIYLAITLISQRVIKVLEGRFTRF
                     ERGVA"
     gene            complement(3917..4642)
                     /locus_tag="PXD71_002181"
     CDS             complement(3917..4642)
                     /locus_tag="PXD71_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL6292256.1"
                     /translation="MKKLLLASAIAFATLSANAQETITFAMEPSYAPFETTNEKGEVI
                     GFDVDIAKALCEELKATCEFKTQSFDSLIAGLKFKNFDAIISGMDITEARQKQVAFTN
                     AYLENSVSYVAMKGKFTPETAKNVGVQNGTTFQQYLSKNAKQYNLKPYASLQSAVLDL
                     KNGRVDMVFGDTPVLAEWLKTEKELDFVGEKVVDKAFFGNGYGIAVNKSNTALLEKLN
                     SALATIKTNGKYEQIYNTWMAGK"
     gene            complement(4668..5396)
                     /gene="artP"
                     /locus_tag="PXD71_002182"
     CDS             complement(4668..5396)
                     /gene="artP"
                     /locus_tag="PXD71_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005697706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDL6292257.1"
                     /translation="MRIQNVNFFYGSNQALFDINLEIEKGDTVVLLGPSGAGKSTLIR
                     TLNLMEVPQSGTLAIANHQFDLTAKTDSKAVSLLRREVGMVFQQYHLWPHLTVMQNLI
                     EAPMKVLGLSKEEAVARAKGHLARLRLSEFAERFPLHLSGGQQQRVAIARALMMQPQV
                     LLFDEPTAALDPEITAQVVDIIKELQSTGITQVIVTHEVGVARKVASKVVYMEKGTII
                     EQGDASCFESPKTEQFAQYLSHSE"
BASE COUNT         1615 a         1192 c         1041 g         1549 t
ORIGIN      
        1 attcattttg ccgggcttat cagccccggc cccgatttct tctatgtcgt cagacaatcc
       61 gccagccact cagttaaaag tggggtactt gccgcaaccg gaatctcaat cggcattatt
      121 ttttgggcag cctttgcgat ttttggttta gcgtggctca gtaatagtat tggctcgctg
      181 tttcaatatg ccattatgat tatcggtggc tgttacctgg tttatattgg ctctcaaatg
      241 gtacgggtaa ccgaaaatat catttttgtt gaaaataaag ctcaactcac gccgctaaac
      301 ccttggcagg aaattaaaaa aggcttatta atcaatatct ttaatgccaa agctggcgtt
      361 tactttacca gcgtcatttc agcctatgta gataatttaa gcaataccct acaaatgttc
      421 gaactacttc tactctttgt ggtaagtacc tttatttatt tctgtgtggt ggcactactc
      481 ttttctcgcc gaccggtcag actgttctat gccaaatata gtcgctatat tgacaatatc
      541 tcaggcgtaa ttttcatctt attcggctcg atgctgattt atgaaggatt cagccacttt
      601 atctaatcac aagcggtctg atttcactaa aaatttgcaa attccgctat caataaatag
      661 tgttatttta tacagtttct gctatgctac tgccaatttt aactctgaga gaacaaaatg
      721 attggacgat tacacggcaa aattattgaa aagcaaccgc ctgaaatttt attagacgtg
      781 caaggggtag gctatgaact actgctcccg atgaccagtt tctacagcct gccacaaatt
      841 ggcgaagagg cgactatttt cacccattta gtggtgcgag aagatgctca cttgctattc
      901 ggctttgccc aaaaacaaga tagaactcta ttccgtgaat taattaaaac caacggtgtc
      961 gggccgaagc tcgcgttggc aattctatca gcaatgtcgg tgtcgcaatt tgccaccgcg
     1021 attgaaaatg aagagctggc aaaattaacc aaaattccgg gaatcggtcg gaaaaccgca
     1081 gaacgcttat tggtggaact caaaggcaaa tttaaaggaa tggcacagag tgatttcttt
     1141 gtggaagtat cgcacgagca aattgtggca agtaatacgc cagacccggc aaatgaagcc
     1201 cttgatgcct taatagccct aggctacaaa cctgccgatg cggaaaaaat gattaagaaa
     1261 gtgaataaat ccggtgcaac gagcgaacag cttattcgtg aggcgttaaa aaattcatta
     1321 tgatattgat agtatagggc gaaagatatt tcgcccctac aatctcattt ttaaaaatat
     1381 atgtgtaggg gcaaatcaat atttgccctc atgagtaata tagggtaata ccattccact
     1441 acaacaataa tctaaatgta tgattgaagc agatagaatc atcagtgcct cgcctaagcg
     1501 tgaggaagaa gttatagacc gagcaatccg cccgaaactg cttgccgact atgtcgggca
     1561 gccttctgtg catgatcaaa tggagatctt catcaaagcc gctaaattgc gggaagaggc
     1621 attagatcac ttgctcattt tcggcccgcc cggtttggga aaaactaccc ttgccaacat
     1681 tgtggcaaac gaaatggggg tgaatattcg cacaacttca gggccggtgc tggaaaaagc
     1741 aggcgatttg gcagcaatgc tgaccaacct tgaaccttac gatgtgctgt ttattgatga
     1801 gattcaccgc ctttctccag cgattgaaga agtgctttat cctgcaatgg aagattacca
     1861 gctcgatatt atgattgggg aaggacctgc tgctcgttcc atcaagctcg atctaccgcc
     1921 ttttaccctt gtaggggcaa ccacccgtgc cggctcactg acctcaccgt tacgagatcg
     1981 ttttgggatt gtacaacgct tagaatttta ctcagtggac gatcttactc tgattgtcaa
     2041 acgcagcgcc gattgcttaa atctcaattt gtcggcagac ggtgcttatg aagtggctcg
     2101 ccgctcccgt ggcacacccc gtattgccaa ccgcttactc cgccgagttc gggattacgc
     2161 cgatgtgcgt aataatggca ttattacctc agacattgcc aaacaagctc tggcaatgct
     2221 cgatgtggat tcggaaggct ttgattttat ggacattaaa ctgttgcaag cgattgtaga
     2281 acgctttgat ggcgggccag ttggattaga caatcttgcg gcagctatcg gcgaagaacg
     2341 agatacgatt gaagatgtgc tagagcctta tctgattcag caaggctttc tacaacgcac
     2401 accaagaggc agaattgcta ccagcagaac ctatgcccat ttaggtataa cccaaataga
     2461 ttagtcatat ttctaaaggc attgatacat aaataaaaag cggatagtta gtactatccg
     2521 ctttcatttg caaaaaatca ataaaatttg accgcttatc cgctaggctt tttcagtctc
     2581 aaaacgctca aaagctaata ctctgctttc taattttctg agaacgattg tcataatgcc
     2641 ggtaattacc aagtaaataa cccctgccat accataaatt gataatgcgt catactctgt
     2701 accataaagc tggcgagcat agcccataat atccataatc gtaatggttg aagcaagcga
     2761 tgtgccttta aaaactagca caatctcatt actataagaa ggtaaagcac gttttaatgc
     2821 gtaaggcact aaaattttca gtgtatctaa acggctcaaa ccaagggctg cgcaagtttc
     2881 ccattgcccc ttcggaatcg cttttaccgc accgtggaac aacaatgtcg aataggcagc
     2941 actgtttaac gctaaagcca acattgcaca gaaccaagcg tcggataata atacccaagc
     3001 cgcactttct gtaatccatt caaattgccc cgggccacta taaatcaaga aaaattgaat
     3061 taataaaggc gttccggtaa aaagcgtaat aaatccgttt accaaccact ttaacggacg
     3121 attttctaac gagagaataa acgtaagaat gatcgctaaa ataaaggcaa tgatgagcgc
     3181 cacaaaagtg agctgtaggc tggtgccaat accctgtgaa atttcaacta aataatccca
     3241 ccacattaag caactcctcg ctcaaaacgg gtaaatctac cttctaatac tttaatcact
     3301 cgctggctaa ttagagtaat cgctaaataa ataagtgctg ctatcccata ccaagtaaaa
     3361 ggttgatgag tattagcatt aattaactcc gattgacgca ttaaatcatc taccccaatt
     3421 aacgatacca atgccgtatc ttttaacagc actaaccact gattgcttaa gcctggtaaa
     3481 gcgtgtcgcc agacttgagg gagaataatg cgaacaaata cttgcgctcg gcttaaccct
     3541 aacgccgtac cagattccca ctgcccaact ggaatagctt taattgcacc acgtaaagat
     3601 tgggaagcat aagcagcaaa aataataccc agcgcaatag ccccacacca gaaagcattt
     3661 aactccgcaa actcatcggt gatcaagaaa ataacttgga taagaccaaa atagatcaaa
     3721 aataccacta aaatctccgg caacccacgc aataatgtaa ttattatact agttggttta
     3781 cggatacatg caatcttact ggtttcaagt accacaaaaa agattgacaa taacattcca
     3841 atcaagaggc ttgccagcgc aatccctaag gtcatcaggg ttgcgttgac aagtaagttt
     3901 aaaatatcgc tagacattat ttcccagcca tccaagtatt ataaatttgc tcatatttac
     3961 catttgtttt gatggtagct aaagccgaat ttaatttttc aagcaatgca gtgttagatt
     4021 tatttaccgc aataccatat ccattaccaa aaaaggcttt atcgactact ttttcaccca
     4081 cgaaatctaa ctctttttcg gtttttagcc actctgccaa tactggtgta tcaccaaata
     4141 ccatatcaac acgtccattt tttaaatcta acactgctga ttgtaggcta gcgtaaggtt
     4201 ttaaattata ttgtttggca tttttgctta agtattgttg aaaagttgta ccattttgca
     4261 cgccgacatt cttagctgtt tccggtgtaa atttcccctt cattgcgaca taacttactg
     4321 agttttctaa ataagcattg gtaaaggcaa cctgtttttg acgcgcttca gtaatatcca
     4381 tgcctgaaat aattgcatca aaatttttaa attttaatcc cgcaattaag ctatcgaatg
     4441 attgcgtttt aaattcgcaa gttgctttta gttcttcaca taatgctttt gcaatatcta
     4501 catcaaagcc aataacttca cctttttcat ttgttgtttc aaaaggggcg taagaaggct
     4561 ccatcgcaaa agtgatagtt tcttgtgcat tggcagaaag cgttgcaaat gcgatagcgg
     4621 aagctaataa taattttttc attgttgtgt ccttttgttg ttatagatta ctctgagtga
     4681 gataaatatt gagcaaattg ctctgttttc ggtgattcaa aacaacttgc atcaccttgt
     4741 tcaataattg tacctttttc catgtacacc actttgcttg ccaccttacg agcaacgcct
     4801 acttcgtgag tcacaatcac ctgagtaatg cctgtagatt gcaattcttt aataatatca
     4861 actacttgtg cagtaatttc cgggtcaagt gctgcggtag gttcatcaaa taataagacc
     4921 tgtggctgca tcatcagtgc tctggcaatc gccacccgtt gttgctgacc acctgaaaga
     4981 tgcagtggga aacgctcggc aaactcgctc aaacgcaaac gtgccaaatg tccttttgct
     5041 ctggcaaccg cttcttcttt gcttaaccca agcactttca tcggggcttc aattaagttt
     5101 tgcattacgg ttaaatgcgg ccataaatga tactgctgga acaccattcc cacctcacgg
     5161 cgtaacaaac tcaccgcttt gctgtcggtt ttagcagtta aatcaaattg atgatttgca
     5221 atcgccaaag tgcctgattg cggtacttcc attaagttta aggttcgaat tagggtactt
     5281 ttacctgccc cactcgggcc aagcaataca acagtatccc ctttttcaat ctctaagtta
     5341 atgtcaaata aggcttggtt tgagccataa aaaaaattga cattttgaat acgaatt
//
LOCUS       DANLXS010000166         5334 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.167, whole genome shotgun sequence.
ACCESSION   DANLXS010000166 DANLXS010000000
VERSION     DANLXS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.167"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(39..1322)
                     /locus_tag="PXD71_002183"
     CDS             complement(39..1322)
                     /locus_tag="PXD71_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6292258.1"
                     /translation="MTACHEPKRSIWQQIFTKNMLLCVYTGFCSGLPLFVLIQLMPAW
                     LTSANLDIKTIAAFTLTSLPYTWKFLWAALLDRYFPPFLGRRKSWIFISQIGLLVILA
                     SFGLFNPVQDISIIVTLSVLLAFLSATQDIVVDAFRREILSDNELGLGNSIHVNAYRI
                     AGLIPGGLSLFLADHYAWDTVFLITAAFLVPCLFVTLIIAAEPNHKPIDRSKPFYMAF
                     VDPFKEFFTRKGVAGAIGLILFIFLYKLGDSLATTLQTKFILDMGFTKSHIAGIVKIT
                     SLWCSIGGGIIGGVAMLKLGVNRALWLFGFVQLITILGFAYLASFEHFPTESIGAAEL
                     WKLGLVMAGEYLGVGLGTAAFVAFMARETNPAYTAMQLAIFTSLAALPSKMLGAYTGH
                     LVEMMGYYQFFWLCFFIGIPGMLMLFKVAPWGQAE"
     gene            1488..5204
                     /gene="metH"
                     /locus_tag="PXD71_002184"
     CDS             1488..5204
                     /gene="metH"
                     /locus_tag="PXD71_002184"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL6292259.1"
                     /translation="MNTQHRTELLQNALTERILILDGAMGTMIQKYKLTESDFRGERF
                     KNSTIDLKGNNDLLTLTQPLLISAIHEKYLAAGADIIETNTFSATTIAQADYELESIA
                     YELNLVGAKLARLAADKFSIPEKPRFVAGILGPTNRTCSISPDVNDPGFRNITFMQLV
                     EAYAEATRGLIEGGADLIMIETIFDTLNAKAAVFAIETVFDELGVKLPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAKPLTFGLNCALGPKELRPYVEMMSKISETFVSVHPNAG
                     LPNAFGGYDLGAEEMATYIKEWAELGWLNIVGGCCGTTPEHIQAFAEATKGVAPRKLP
                     EIPTAMRLSGLEPLTIDDNSLFVNVGERNNVTGSAKFKKLIKEEKFAEAIEIAISQVE
                     NGAQVIDVNMDEALLDSKKCMTRFLNILATEPEAAKVPIMIDSSKWEVIEAGLQTVQG
                     KPIVNSISLKEGEEKFIHQAKLCRRYGAAVVVMAFDEVGQADTEERKVEICTRAYRIL
                     VDQLGFPPEDIIFDPNIFAIATGIEEHNNYGVDFIQACERIKRDLPHAKISGGVSNVS
                     FSFRGNNVVREAIHAVFLYYAIKAGMDMGIVNAGQLAIYDDLDPELREAIEDAVLNHR
                     HDTTDRLLEISEKYRGVKVESADESAAEWRNLPVAERLKHALVKGITTYIIEDTEEAR
                     QQFASPLEVIEGPLMAGMDVVGDLFGDGKMFLPQVVKSARVMKQSVAYLEPYINATKQ
                     KGSSNGKMVIATVKGDVHDIGKNIVSVVLQCNNFEVIDLGVMVPADVIIETAIREKAD
                     LIGLSGLITPSLDEMEYFLGEMNRLKLDIPVLIGGATTSKEHTAIKLYPKYNYPVVYT
                     ANASRCVTVCATLMNPEAKAEFWEKTKAEYEEIQRKFAVRKPLRNGLSIEEARANRFD
                     GFSGEWADYVPPTPKQTGIIEYKNVPIATLRKFIDWSPFFMLWGLMGGYPDAFDYPEG
                     GEEARRVYNDAQRVLDEFEQNGKLNPSGIMGIFPAVREGDDIAILNEDRTACIGKSYH
                     LRQQSERGKNSKSPYNLCLSDFIADRESGQQDWFGMFAVCAGIEEHYLVESYKAAGDD
                     YNAILLQAVGDRLAEAMAEYLHFELRTKVWGYTVEDEDNGRLIREEYIGIRPAPGYPS
                     CPEHTEKQIIWDLLEVEQRIGMKLTESYAMWPAASVCGWYFTHPTSNYFTLGKIDEDQ
                     VLDYAKRKGWDELTAKKWLSVAMKQDVKSQAENI"
BASE COUNT         1567 a         1226 c         1297 g         1244 t
ORIGIN      
        1 gtcggatatt taataaaaaa tcttattttt atgaatagct attcagcttg tccccaagga
       61 gcaaccttaa acaacattag cattcccgga ataccaataa agaaacagag ccagaagaat
      121 tggtaatagc ccatcatttc cactaaatgc cctgtgtatg cccctaacat tttgcttggt
      181 agggcagcaa ggctggtgaa aatggcgagc tgcattgcag tgtaagcggg attagtttca
      241 cgagccataa aggcaacgaa agccgctgtg cctaaaccca cgcccagata ttcacctgcc
      301 atcactaagc ctaatttcca aagctcggca gctccaatgc tttcggtagg gaaatgctca
      361 aaactggcaa ggtaagcgaa gcctaaaata gtaattaatt gtacaaatcc gaacagccat
      421 aaggctcggt taacgcccaa tttcaacatt gccacgccac caataatacc gccgccaatg
      481 ctgcaccaaa gtgaagttat tttcacaatg ccggcaatgt gcgatttagt aaaacccata
      541 tccaaaataa atttggtttg tagcgtagtt gcaagggaat cgcctaattt atataggaaa
      601 ataaatagaa tcagcccaat cgctcctgcc acgcctttac gggtgaaaaa ctctttgaac
      661 ggatcgacaa acgccatata aaacggctta gaacggtcga ttggcttatg attcggctcg
      721 gcagcgatta ttagcgtaac gaataagcaa ggcactaaaa aagcagcggt aatcagaaat
      781 acggtatccc acgcatagtg atcggctaaa aataatgaaa gcccacccgg aatcagccct
      841 gcaatacgat aagcattaac gtggatagag ttacctaacc cgagttcgtt atccgataaa
      901 atttcacgcc gaaaggcatc caccacaatg tcttgcgtgg cggaaagaaa agctaatagc
      961 acagaaagag taacaataat tgaaatatct tgcactgggt tgaacaagcc aaagctggct
     1021 aaaatcacca atagcccgat ttgtgaaatg aaaatccagc ttttgcgtct gcctaaaaaa
     1081 ggtggaaagt agcgatctaa caatgcagcc cacaggaatt tccacgtgta gggcaaagag
     1141 gtcagggtga aggcggcaat agttttaata tctaaattgg cactggttag ccaagccggc
     1201 attagctgaa ttaacacaaa caacggtaaa ccggaacaaa agccggtgta aacacaaagc
     1261 aacatatttt tagtaaaaat ctgttgccag atagagcgtt tgggttcgtg acaagcggtc
     1321 atatttttcc tacattttgc aaaataaaaa ggggaagaac tgagccaata tagcataatt
     1381 ttaaaggcga ctctaaccct ttccatttcg tcacagataa ggtttgacag ccttgcgaca
     1441 tatttctatt attcctttta atcaaactaa aaaactggaa gcagagtatg aacacgcagc
     1501 atagaaccga attacttcaa aatgccctta ccgaacgcat tcttatctta gacggtgcga
     1561 tgggaacgat gatccaaaaa tataaactaa ccgaatctga tttccgaggt gaacgtttta
     1621 aaaacagtac catcgatctt aaaggcaata acgacctact cacactcacc cagccactcc
     1681 tgatttctgc tattcacgag aaatatttag ccgccggtgc ggatattatc gaaactaata
     1741 cctttagtgc gacgaccatt gctcaagcag attatgaact tgaaagtatt gcttatgagc
     1801 tgaatcttgt gggggcaaaa cttgcccgtc ttgccgccga taaattctcc atccctgaaa
     1861 aacctcgttt tgttgccggt attcttgggc cgactaaccg tacttgttcg atttcccctg
     1921 atgttaatga ccccggtttc cgtaacatta cctttatgca actggtggaa gcctatgccg
     1981 aggcaacccg tggtttgatt gaaggtgggg cggatctgat tatgatcgaa accattttcg
     2041 ataccctgaa tgccaaagcg gcggtgtttg caattgaaac ggtgtttgat gaactgggcg
     2101 tgaaattgcc gataatgatc tccggcacca ttaccgatgc ctcagggcga acgctgtcag
     2161 ggcagaccac ggaggcgttt tataactcgc tccgccacgc caaaccgctg acgttcggct
     2221 tgaactgtgc gctcgggccg aaggaactcc gcccttatgt agagatgatg tcgaaaatca
     2281 gtgagacttt tgtttcggtt cacccgaatg ccggcttgcc aaacgctttt ggcgggtacg
     2341 atttaggggc ggaagagatg gcgacttaca tcaaagagtg ggcagaactc ggctggctga
     2401 atattgtcgg cggctgttgt ggcaccacgc cggagcatat ccaagccttt gccgaagcaa
     2461 ccaaaggcgt tgcgcctcgt aaattacctg aaattccaac cgctatgcgt ttgtcgggct
     2521 tagaaccgct gacgattgac gataattcgc tgtttgtgaa tgtgggcgaa cgcaataacg
     2581 tcaccggttc ggcaaaattt aagaagctga ttaaagaaga aaaatttgcg gaagcgattg
     2641 aaattgccat cagccaagtg gaaaacgggg cgcaggtgat tgacgtgaat atggacgagg
     2701 cgttgctcga ttccaaaaaa tgtatgaccc gtttcctcaa cattcttgct accgaacctg
     2761 aggcggcaaa agtgccgatt atgattgact cctcgaaatg ggaggtgatc gaggcgggct
     2821 tgcaaacggt gcagggcaag ccgatagtga actccatttc actcaaagag ggcgaagaga
     2881 aatttattca ccaagccaaa ctctgccgcc gctatggggc tgcggtggtg gtgatggcat
     2941 ttgacgaagt cgggcaagcg gataccgaag aacgcaaggt agaaatttgt acccgagctt
     3001 atcggatttt ggttgatcag ctcggcttcc caccggaaga cattattttc gacccgaaca
     3061 tttttgcgat tgccaccggt attgaagaac acaataacta cggcgtggat tttatccaag
     3121 cctgtgagcg gattaaacga gacttgccac acgccaaaat ttccggcggg gtgtcgaatg
     3181 tctctttctc tttccgaggc aacaatgtgg tgcgtgaggc tattcacgcc gtgttcctct
     3241 actatgcgat caaagccgga atggatatgg ggatcgtgaa cgcagggcag ttggcgattt
     3301 atgacgacct cgaccctgaa ctgcgagagg cgattgaaga tgcggtgctg aaccaccgtc
     3361 atgacactac cgaccgcctg ttggaaattt ccgaaaaata tcggggcgta aaagtagaaa
     3421 gtgccgatga aagtgccgcc gagtggcgaa atttacctgt ggcagagcgt ttaaaacacg
     3481 ccctcgtaaa aggcataacg acctacatta ttgaagatac cgaagaggct cgccagcaat
     3541 ttgcctctcc gctggaagtg attgaagggc cgctcatggc gggtatggac gtggtgggcg
     3601 acctgttcgg agacggcaaa atgttcctgc cgcaagtggt gaaatcggct cgggtaatga
     3661 aacagtccgt tgcctattta gagccgtaca tcaacgccac caagcagaaa ggatcgagca
     3721 acggcaaaat ggtgatcgcc accgtgaaag gtgatgtaca cgacatcggc aaaaacatcg
     3781 ttagcgtggt gttgcagtgc aataactttg aagtgataga cttaggcgtg atggtgcctg
     3841 ccgatgtaat tatcgaaact gctattcggg agaaagccga tctcattggg ttaagcggac
     3901 taattacccc atcgctggac gaaatggagt attttttagg tgaaatgaac cgcttgaagc
     3961 tcgatattcc ggtgctgatt ggcggcgcaa cgacctcaaa agagcatacg gcgatcaaac
     4021 tttatccgaa atataactac cccgtagtct ataccgccaa cgcttcacgt tgcgtaaccg
     4081 tctgtgcgac cttgatgaac cctgaggcaa aagccgaatt ttgggaaaaa accaaagccg
     4141 aatacgaaga aattcagcgt aaatttgccg ttcgcaaacc acttcgcaac ggtttatcta
     4201 ttgaggaggc tcgagccaat cgttttgacg gatttagtgg cgaatgggca gactatgtgc
     4261 cgccaacgcc aaaacagacc ggcattatcg agtacaaaaa tgtaccgatt gccaccctac
     4321 gcaaatttat cgactggtcg ccgttcttta tgctctgggg cttaatgggc ggttatcctg
     4381 atgcctttga ttatccggaa ggcggcgaag aggcacgccg tgtctataac gatgcccaac
     4441 gggtgctgga cgaatttgag caaaacggca agctcaaccc aagtgggatt atgggcattt
     4501 tccctgccgt gcgtgagggc gatgatattg caattttaaa cgaagatcgc accgcttgta
     4561 tcggcaaatc ctaccacctc cgccaacaat ccgaacgagg caaaaacagc aaaagcccgt
     4621 acaacctctg tttaagcgat tttatcgccg accgagaaag cggacaacaa gactggttcg
     4681 gaatgttcgc cgtttgtgcc ggcattgaag aacactattt agttgaaagc tacaaagcgg
     4741 caggcgatga ctacaacgcc attttgctcc aagccgttgg cgaccgcttg gcagaagcaa
     4801 tggcggaata cctacacttt gagctacgta ccaaagtctg gggctacacc gtggaagacg
     4861 aagacaacgg acgcttaatc cgtgaagaat acatcggcat ccgccctgcg cccggctacc
     4921 caagctgccc ggaacacacc gagaaacaga taatttggga cttgttagag gtggaacaac
     4981 gcatcggcat gaaactcacc gaaagctatg ccatgtggcc ggcagcaagt gtctgcggct
     5041 ggtacttcac ccaccctacc agcaactact tcactctcgg caagattgat gaagatcagg
     5101 tgttggatta cgccaaacgc aaaggctggg acgaactcac ggcgaagaaa tggttaagtg
     5161 tggcgatgaa acaagatgtc aagtcgcagg ctgaaaacat ttaatgtagt ggcgggtttt
     5221 aacccgcctg ttacaagcgg ttggttttga gataaaatat gcaaaattta tctctctttt
     5281 atgcaaagaa gttatcatcg agaacaaaac aatgattgaa aaacaaaatt atca
//
LOCUS       DANLXS010000167         5319 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.168, whole genome shotgun sequence.
ACCESSION   DANLXS010000167 DANLXS010000000
VERSION     DANLXS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.168"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..170)
                     /locus_tag="PXD71_002185"
     CDS             complement(<1..170)
                     /locus_tag="PXD71_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL6292260.1"
                     /translation="MNLIICCTPLQVLIAEKIIEMHPNERFYGVMLSTVKNAKFDFYQ
                     ARLAQKCEQFFA"
     gene            complement(167..1372)
                     /locus_tag="PXD71_002186"
     CDS             complement(167..1372)
                     /locus_tag="PXD71_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HDL6292261.1"
                     /translation="MKAVKDSVIYLIGELASKSIPFLLLPYFSRKLGVAGYGELSYYQ
                     TFAALFVIFIGLSQDGAVARYFYFYGKRSLNLVLSTGYLYTLCATLLILLGCWYFQSE
                     IIAYVAIGAMFQSFLNVQLSVRQCQKQALAYTTIQLMSAIFSAVITVAMMEYFKTDLV
                     EKRILAILMSNGLVFLIAYFLYAKRAKTKKFSLARHKTALLYLFGFGIPLILHHSSIF
                     LKGQLDRVFIYHKFSEEDLGLYAMGAQIAMILAIVVQALNKATLPYFYEALKQKKINV
                     SHIHKWALYALFIVPLPAIVMWFIPEELVVWILGSQFVGTKYYIILFLIATMLVVPYL
                     ILVNYLFFYGKNKLISLCSILSTIFYLVALFALTFSKIEYIPFASIIGAISIIPILYI
                     MTAKVGKTV"
     gene            complement(1382..2566)
                     /gene="neuC"
                     /locus_tag="PXD71_002187"
     CDS             complement(1382..2566)
                     /gene="neuC"
                     /locus_tag="PXD71_002187"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL6292262.1"
                     /translation="MKNIAYITGSRAEYGIVKRLLQKLAQDPTVNFSLVVTAMHLDPQ
                     YGNTVTVIEQDGFEIAARIPLTLNSENNQTIIHSMAECLDRFGEHFQQHRYDAVIVLG
                     DRYEMLAVATAAAMHNIPLIHLHGGEQTLGNYDEFIRHCITKMSKLHLTSTEEYRQRV
                     IQMGELPENVVNTGSLGAENSLDLVLPTQAELVERLGIPASPYFLVVFHPETLTDVSV
                     LEQIEALLNAFEQFKDKYQFVFIGSNSDTNSEQIYARFKAYTDENQFKFFTSVKPEEY
                     LALIKYSNGLIGNSSSGLIEAPSCGVGTLNIGNRQQGRVRDESVIDVESNEQAIIDGI
                     EKLISADFQHRLPSMKNPYFQDNAMEKAYNAIKIFLADSHHTQPKGFYDVAFSQAVKK
                     SE"
     gene            complement(2567..3826)
                     /locus_tag="PXD71_002188"
     CDS             complement(2567..3826)
                     /locus_tag="PXD71_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL6292263.1"
                     /translation="MKKIAIITARAGSKGLPNKNVLLANGKPLMAYSIEAAIESGQFE
                     KIIVSTDSQEYIDLFSHYPIEFIKRAEHLASDKASSFVVIEDVLNQYRSVEFDYFMLL
                     QPTSPLRSGEHIKESCEKFEKNFEQFDFLVSVSDAHKPTTLTRMIEEDECLKHFQLDY
                     SNYARQLHHPEYSPNGAIFTAKPEAYLKQKHFYGAKCLAYFMDKDVSIDIDDRQDFEY
                     FYFILQQRNKEKILLETIKRSILMKKENFKEVKEIALIGHSIIEYWDIEHLNGKAVNN
                     LGIAGISTKQYSDLILDEGLISALPKDIILMFGTNDIVYAGWSDEKILADINHLIAQL
                     TAIRGDINLYFLEITPTAFRVDRNNNDIRLLNQYLKDNLAVKWISLDQAFSDKYGKLD
                     LSYSDDGLHLNAKGYEKLTAILEKELN"
     gene            complement(3886..4923)
                     /gene="neuB"
                     /locus_tag="PXD71_002189"
     CDS             complement(3886..4923)
                     /gene="neuB"
                     /locus_tag="PXD71_002189"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL6292264.1"
                     /translation="MVNVFIVAEIGCNHNGDPALAKKMVDVAKECGVDAVKFQTFKAD
                     KLISKYAPKAEYQKVTTGTADSQLEMTRKLELPYEEFVKLEAYARSLGLEVFSTPFDF
                     ESIDFLASQQQKVWKIPSGELTNLPYLEKIARLPIEGKTIVISTGMATIEETKASLKV
                     LEDNGVAKKDITILHCNTEYPTPYEDVNLNAFHQLKQEFGDYSLGFSDHSAGYFAGLA
                     AVPYGITFIEKHFTLDKNFEGPDHKASVTPEELKLLCEGIRAVEKSLGSSEKLVTNSE
                     AKNKIVARKSIIAARPIKKGEIFTTENITTKRPGNGISPMFWYDVLGKEAQQDFEEDQ
                     LIQDSRFENQM"
     gene            complement(4923..>5319)
                     /locus_tag="PXD71_002190"
     CDS             complement(4923..>5319)
                     /locus_tag="PXD71_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL6292265.1"
                     /translation="KLQKYKCRIINVVDKTALVSKNSTLGIGVFVGKMAIVNSGVTVG
                     DNVIINTKSLVEHGCFIGSHCNISTNTTLNGDVIVEDHAFIGSSSVVNGQLRVGESAL
                     VGSGAVVIRNVEPRTVVAGVPAKYIKDIK"
BASE COUNT         1674 a         1114 c          884 g         1647 t
ORIGIN      
        1 atcgcaaaaa attgttcgca cttttgggct aatctagctt ggtaaaaatc aaatttcgcg
       61 ttttttaccg tagaaagcat caccccatag aatcgctcat tagggtgcat ttcaatgatt
      121 ttttcagcaa tcaacacttg caagggcgtg caacaaataa ttaaattcat acggtttttc
      181 ctacttttgc cgtcattata taaagaatcg gaataatgct gattgcacca atgatgctgg
      241 caaaaggaat atattcaatt ttagaaaaag ttaaagcaaa tagtgcaact aaataaaaaa
      301 tagtagataa aatcgagcaa agcgaaatta gcttattttt gccataaaaa aacaaataat
      361 tgactaaaat gaggtaaggc acaaccaaca ttgtcgcaat taaaaagaga ataatgtagt
      421 attttgttcc tacaaattgg ctacccagaa tccataccac taattcttcc ggaataaacc
      481 acatcacaat agcaggtaat ggtacaataa ataaggcata caacgcccat ttgtggatat
      541 ggctgacatt aatttttttc tgctttaagg cttcataaaa ataaggcagt gttgctttat
      601 ttaaggcttg aaccacaatg gctagaatca tcgcaatttg agcccccatt gcatataaac
      661 ctaaatcctc ttcgctaaat ttatggtaaa taaatactct gtctaactgt ccttttaaga
      721 aaatactgga atggtgcaaa atcaacggaa taccaaagcc gaataaatac aataaagcag
      781 ttttatggcg ggcaaggcta aatttcttcg tttttgctct tttagcatac aaaaagtaag
      841 cgattagaaa gactaagcca ttactcatca aaatcgctaa aatccgcttt tccactaaat
      901 cggtcttaaa atactccatc attgcaacgg taataactgc cgaaaaaatc gccgacatta
      961 attgaatcgt tgtataagca agtgcctgtt tttgacactg ccttacacta agctgtacat
     1021 tcaaaaacga ctggaacatc gccccaatcg ctacataagc aataatttca gactggaaat
     1081 accagcaacc caacaggata agcaaagttg cacaaagagt atataaatac cctgtactca
     1141 agactaaatt tagtgaacgc ttaccataaa aatagaaata gcgagcaacc gcaccgtctt
     1201 ggctaagccc gataaaaatt acaaataaag cagcaaaggt ttggtagtag gaaagctccc
     1261 cataacctgc cacgccgagt ttgcgtgaaa agtagggtaa gagcaagaaa ggaattgact
     1321 ttgacgccaa ttccccaatt aaataaatca cactgtcttt aactgctttc atagttgcaa
     1381 attattctga ttttttgacc gcttgcgaga aagcaacatc ataaaatcct ttcggttgag
     1441 tatggtggct gtctgctaag aatattttga ttgcattgta agccttttcc atcgcgttgt
     1501 cttgaaaata tggatttttc atactcggta aacggtgttg gaaatcggct gaaatcaatt
     1561 tttcgatacc atctatgata gcctgttcat tactctctac atcaattacg ctctcatccc
     1621 gtactcgccc ttgctggcga ttaccgatat ttagcgtacc aacaccacaa ctcggtgctt
     1681 caatcaagcc tgatgagcta ttaccaatca acccatttga atatttaatc aacgctaaat
     1741 actcttccgg tttgactgag gtgaaaaact taaattgatt ttcatcggtg taagccttaa
     1801 aacgtgcata aatttgctct gaattggtgt ctgaatttga gccgataaag acgaattggt
     1861 atttgtcttt aaactgctca aaagcattga gtaacgcctc aatttgctcc aacacggaaa
     1921 catcggttaa ggtttccggg tggaagacca ctaaaaaata aggcgatgcc ggaatcccta
     1981 atctctcgac taattctgct tgggtcggca acactaaatc caaactattc tccgccccta
     2041 gagaaccagt attcaccaca ttttcgggca gctcgcccat ttgaatcaca cgctgtctat
     2101 attcttcagt tgaagttaaa tgcaatttcg acattttggt aatacaatgg cgaataaatt
     2161 catcgtaatt acctaatgtt tgctcaccac cgtgcagatg aatcagcgga atattatgca
     2221 tcgccgcagc cgttgccacc gccaacatct cgtaacgatc gcctagcaca atcaccgcat
     2281 cataacgatg ttgctggaaa tgttcgccaa atcgatctaa acactctgcc attgaatgaa
     2341 taatggtctg attattttca ctgtttaagg taagcggaat acgagctgca atctcaaagc
     2401 catcttgctc aatgaccgtc accgtattgc catattgcgg atccaaatgc atcgcagtaa
     2461 caaccagaga gaaattcaca gtcggatctt gtgctaattt ttgtaataaa cgctttacaa
     2521 tcccgtattc ggcacgagat ccggtaatat aggcaatatt tttcatttag ttgagctctt
     2581 tttccaaaat agcggttaat ttttcataac cttttgcatt taaatgtaat ccgtcatcgc
     2641 tatagcttaa atccaatttg ccatatttat cgctaaatgc ctgatctaag ctaatccatt
     2701 tcaccgctaa attatcttta agatattgat ttaacaagcg aatatcgttg ttattacgat
     2761 cgactctaaa tgcagttggg gtaatttcca agaaatagag attaatatct ccacgaattg
     2821 ctgttaattg tgcaattaaa tgattaatat ctgctagaat tttttcatca ctccagccag
     2881 catatacaat atcattagta ccgaacatta aaataatgtc tttaggtaaa gcagaaatta
     2941 aaccttcatc caaaatcaga tcactgtatt gttttgttga aatacctgca attcctaaat
     3001 tattaactgc cttaccgttt aaatgttcaa tatcccaata ttcaatgatc gaatgcccga
     3061 ttaaggctat ctctttcact tctttaaagt tctctttttt cattaaaatc gaacgtttaa
     3121 tggtttcaag taagattttt tctttattgc gttgttgcag aataaagtag aagtactcga
     3181 aatcttggcg atcatcaata tcaatagaaa cgtctttatc cataaaatag gctagacatt
     3241 tcgcaccata gaagtgtttt tgtttaagat aggcttctgg cttagctgta aaaatagctc
     3301 cattcggtga atattcagga tgatgcaatt ggcgtgcata gttagaataa tccaactgaa
     3361 aatgctttag gcactcgtct tcctcaatca tacgagtcaa tgtcgtaggt ttatgggcat
     3421 cagaaacaga tactaagaaa tcaaattgtt cgaaattttt ctcaaatttt tcacacgatt
     3481 ccttaatatg ctctcctgat ctcaacggcg aagttggttg aagtagcata aaatagtcaa
     3541 actctactga tctatattga tttaacacat cttcaatcac gacaaaagaa ctcgccttat
     3601 cactggctaa atgctcggca cgtttaataa attcaattgg ataatgtgag aacaaatcaa
     3661 tgtattcttg ggaatcagta ctaacgataa ttttctcaaa ttgccccgac tcaatcgcag
     3721 cttcaataga ataggccatt aacggcttac cgttggctaa taatacattt ttattcggca
     3781 agccttttga accggctcgg gctgtaataa ttgcaatttt tttcataaat ttgaccgctt
     3841 gttaaacaca aaatgatgga gctttcatcc catcactata ttatattaca tctgattttc
     3901 aaaacgagaa tcttgaatta attgatcttc ttcaaaatcc tgttgtgctt ctttacctag
     3961 cacatcatac caaaacatcg ggctaatgcc attaccgggg cgtttagtcg taatattttc
     4021 ggttgtaaaa atttcgcctt ttttaattgg acgtgccgca ataattgatt ttcttgccac
     4081 aattttattt tttgcttccg aattagtgac taatttctcg gaagaaccca gcgatttttc
     4141 caccgcacga atgccttcac acagtaattt cagctcttca ggtgtaaccg aagctttatg
     4201 atccggacct tcaaagtttt tatctaaggt aaaatgtttt tcaatgaagg taatgccgta
     4261 aggtactgcc gccaaccctg caaaataacc ggcagagtgg tcagaaaaac ctaaactata
     4321 gtcgccaaac tcctgtttta gctgatggaa cgcatttaaa ttcacatctt cataaggtgt
     4381 cgggtactcg gtattacaat gtaaaatcgt aatatctttt ttcgctacac cgttatcttc
     4441 caatactttt aatgaggctt ttgtttcttc aatggttgcc ataccggtag aaataacgat
     4501 cgtcttgcct tcaatcggaa gtcgtgcaat tttttctaaa tacggcaaat tggttaattc
     4561 accagacgga attttccaca ctttttgttg ttggcttgcc aagaaatcaa tcgactcgaa
     4621 atcgaatggc gtagagaata cctctaaccc caatgaacga gcgtaagcct ctaatttcac
     4681 aaactcctca taaggtaact ctaatttgcg ggtcatttct aattgggaat cagcggtgcc
     4741 ggtcgttact ttttgatact cggctttagg ggcatattta gagatcaact tatccgcttt
     4801 aaaggtttgg aatttcaccg catcgacacc acactctttc gctacatcaa ccattttctt
     4861 tgctaacgca ggatcgccgt tatggttaca accgatttct gccacaataa atacatttac
     4921 cattatttta tatccttaat atattttgct ggaacgcctg ctacaactgt gcgaggctcc
     4981 acatttctaa ttaccactgc accggagcca accaatgcac tttcacccac tcttagctgt
     5041 ccgttcacaa cagaggaact accaataaaa gcatgatctt cgacaattac atcgccattt
     5101 agtgtcgtat tggttgaaat attgcaatga ctgccgataa aacaaccatg ctcaaccagc
     5161 gatttagtgt taatgatcac attatcgcct acagttacgc cactgtttac aattgccatt
     5221 tttccaacaa acactccaat gcctagagtg gaatttttgg acaccaatgc ggttttatct
     5281 accacattaa ttattctaca cttatatttt tgtagctta
//
LOCUS       DANLXS010000168         5257 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.169, whole genome shotgun sequence.
ACCESSION   DANLXS010000168 DANLXS010000000
VERSION     DANLXS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.169"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            917..1054
                     /locus_tag="PXD71_002191"
     CDS             917..1054
                     /locus_tag="PXD71_002191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292266.1"
                     /translation="MKPLRIGARVIYPLQDLDKQIAYLEANLLENIKVHFIVESIEIK
                     G"
     gene            1189..2106
                     /locus_tag="PXD71_002192"
     CDS             1189..2106
                     /locus_tag="PXD71_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class A beta-lactamase
                     ROB-1"
                     /protein_id="HDL6292267.1"
                     /translation="MLNKLKIGTLLLLTLTACSPNSVHSVTSNPQPASAPVQQSATQA
                     TFQQTLANLEQQYQARIGVYVWDTETGHSLSYRADERFAYASTFKALLAGAVLQSLPE
                     KDLNRTISYSQKDLVSYSPETQKYVGKGMTIAQLCEAAVRFSDNSATNLLLKELGGVE
                     QYQRILRQLGDNVTHTNRLEPDLNQAKPNDIRDTSTPKQMAMNLNAYLLGNTLTESQK
                     TILWNWLDNNATGNPLIRAATPTSWKVYDKSGAGKYGVRNDIAVVRIPNRKPIVMAIM
                     STQFTEEAKFNNKLVEDAAKQVFHTLQLN"
     gene            2117..3034
                     /locus_tag="PXD71_002193"
     CDS             2117..3034
                     /locus_tag="PXD71_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL6292268.1"
                     /translation="MKKKLLWILILGLIIISCKQRKTEMKEKIIKTNGIELCTESFGN
                     KKNPAILLVAGATVSMLYWDTEFCQQLSEKGFFVIRYDNRDVGKSTNYEPGSTPYDIV
                     DLTNDAISILDGYKIDKAHFVGISLGGLISQIASIKFADRVNSLTLMSSGPWGDSDPT
                     IPEMDTSILDFHSKAGTVNWTNEDSVVNYLIQGAELMSGKKQFDKQRSEKLIRAEFNR
                     ANNYISMFNHAALQGGGEEYWNRLNEIKQPTLIIHGTDDKIWHYKNAGFLLEKIKGSN
                     LITLEGTGHELHVDDWKSIIDGIEKHIND"
     gene            complement(3359..4765)
                     /locus_tag="PXD71_002194"
     CDS             complement(3359..4765)
                     /locus_tag="PXD71_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL6292269.1"
                     /translation="MIVKFFKKHGKGKASSCKACVDYLLNKPDDTAQILQGDPRLSQS
                     IADSLDFNNTYTAGCLSFEESDLPEIQKREIMARFEKAMFAGLEPEQYNIAWVQHTDK
                     GRLELNFVIPNVEMTSGKRLQPYYDRADRPLAENFKQVINHEYSLSDPNNPIKQKNLI
                     DRKDLPTDKKQALQAITDGLTALANAGQINDRQDVINALERAGFEIARITPKNLSIKT
                     DGQNLRLKGAFYEQDFRFSTDLSADITERAREYKRDSAERYQTARAKLDTAVTARREQ
                     FSRKYPNRAGEIDKKYRENVSLADPNRLDDISLDSHNRRRFDVAGEERLSRDDRMAKF
                     SRDSQRAEWGDQAINLQNGERDKALCSSGQGFYQLNVGREPESNRNPRNQETGEKING
                     DRTRTTLRENLENLARTARNRASEIIERIRELASRKRLDHSTVQRNQRAINDITEFNK
                     QLETVISQRKRQSRGMSR"
     gene            complement(4762..5067)
                     /locus_tag="PXD71_002195"
     CDS             complement(4762..5067)
                     /locus_tag="PXD71_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL6292270.1"
                     /translation="MKREKEIKIRLTENEYQALLERKTKARLAEWVREVALEQQPKRQ
                     PKVIDPALLFELNRIGVNLNQIARQCNSQKPSIDLVSVLATLREIEKNLKKLRELSL"
BASE COUNT         1484 a         1070 c         1074 g         1629 t
ORIGIN      
        1 ttttagtgtt ggaaagctgt tggaaagctc gtgggtaact cgcagagtta tccatcagct
       61 tttcaatggc ttttcaatac gacccgcagg ggaaaaaccg ttaaaccgtc cacattaacc
      121 gctgacccaa aaagaacgct ttttactaaa agatttctat tcggttctca aatgttgcgt
      181 gaatggggca acaataaaac gttgtggcgt ttatcagggt gggaattgca gacttaaaca
      241 atcaattccg ctgtatcctt aaatctcgct tatcaggtgt gcttgtattt cttggggctt
      301 tcgcctttct aaccgtagca cgaaacggag acagagctta attttcgtat taagaacgtt
      361 atcacttcta cgcctgttta ttcttccgaa gtccgagctg aagaccgtct aatttaaggt
      421 ttacgtttct tgtttacgtc gttgaatggt tttttggtag tagggacgct tcgcaatctc
      481 ttgcattact agctaccctt tgctatgatt agattgttca cgtctggatc atagcaaaga
      541 acgtactgat acagattata ggaaaaggga tcgaatgtca aaagttcgat ccctttttct
      601 tttctaaagc attggtatta aaggctttaa ggtgtatttt attaaaatct gtacaccttt
      661 gtgaaaatcc atacaccttt cttaccgtgt aattttatga aaattcgtgc acctttctgc
      721 taacgccctg cggttgttag actaaaaaag ccattgaaag caagtttcaa cggctttttc
      781 agtcccgaat aaaacaaaac cgccttgtta ggcggttatg tgttaaaatc actttcgcac
      841 tatggcggcg gctcgcacta ccgttcatag tgtgagccta gtcgcttagc cttatagtgc
      901 taagggggtg atttatgtga aacctctacg aattggggca agggttattt atccgcttca
      961 agacttagac aagcaaattg catacctaga ggcgaactta cttgagaata tcaaagtgca
     1021 tttcattgta gaaagcattg agattaaagg ctaaattgct agacttttgc gcttaaattc
     1081 gccaaaatct gtttttttgt tctacaaaca aattatccga atttccgcgc aatgtacgtt
     1141 tcaggctgcc tgaaaagaaa tttatttagt aaaatcaaag gataatttat gttaaataag
     1201 ttaaaaatcg gcacattatt attgctgaca ttaacggctt gttcgcccaa ttctgttcat
     1261 tcggtaacgt ctaatccgca gcctgctagt gcgcctgtgc aacaatcagc cacacaagcc
     1321 acctttcaac agactttggc gaatttggaa cagcagtatc aagcccgaat tggcgtttat
     1381 gtatgggata cagaaacggg acattctttg tcttatcgtg cagatgaacg ctttgcttat
     1441 gcgtccactt tcaaggcgtt gttggctggg gcggtgttgc aatcgctgcc tgaaaaagat
     1501 ttaaatcgta ccatttcata tagccaaaaa gatttggtta gttattctcc cgaaacccaa
     1561 aaatacgttg gcaaaggcat gacgattgcc caattatgtg aagcagccgt gcggtttagc
     1621 gacaacagcg cgaccaattt gctgctcaaa gaattgggtg gcgtggaaca atatcaacgt
     1681 attttgcgac aattaggcga taacgtaacc cataccaatc ggctagaacc cgatttaaat
     1741 caagccaaac ccaacgatat tcgtgatacg agtacaccca aacaaatggc gatgaattta
     1801 aatgcgtatt tattgggcaa cacattaacc gaatcgcaaa aaacgatttt gtggaattgg
     1861 ttggacaata acgcaacagg caatccattg attcgcgctg ctacgccaac atcgtggaaa
     1921 gtgtacgata aaagcggggc gggtaaatat ggtgtacgca atgatattgc ggtggttcgc
     1981 ataccaaatc gcaaaccgat tgtgatggca atcatgagta cgcaatttac cgaagaagcc
     2041 aaattcaaca ataaattagt agaagatgca gcaaagcaag tatttcatac tttacagctc
     2101 aactaacaaa ttcagcatga aaaaaaaact actttggata ttaattttag gactgataat
     2161 aatcagttgc aaacaaagga aaacagaaat gaaagagaaa ataattaaaa caaacggcat
     2221 tgaactctgt acggaaagtt ttggaaataa gaaaaatcca gcaatccttt tggtagcagg
     2281 tgcaaccgta tcaatgctgt attgggacac tgaattttgc caacaattat ctgaaaaagg
     2341 attttttgtt attcgttacg acaacagaga tgtaggaaaa tccactaatt atgaaccagg
     2401 ttctactcca tacgatattg ttgacttaac taatgacgct atttcaatat tggatggcta
     2461 caagattgac aaagcacatt ttgtggggat ttctttgggc ggactaattt ctcaaatagc
     2521 atcaataaag tttgccgaca gagttaactc cttaactctt atgtcatcag gcccttgggg
     2581 agactcagac ccaactatac ctgaaatgga cacgagtatt ttagatttcc atagtaaagc
     2641 aggtacagtc aattggacaa atgaagacag tgtggtaaac tatttaattc agggtgcaga
     2701 attaatgagt ggcaagaaac aatttgacaa acaaagaagt gaaaaactga taagagctga
     2761 gttcaataga gccaacaatt atataagtat gttcaatcac gctgcattgc aaggtggtgg
     2821 tgaagaatat tggaacagat taaacgaaat caaacaaccc accttaatta tccacggaac
     2881 agacgacaaa atttggcatt ataagaatgc aggtttttta ctagaaaaaa taaaaggttc
     2941 aaatctaatc acccttgaag gtacaggaca cgaattacac gttgatgatt ggaaatcaat
     3001 aattgatgga atagaaaaac acataaatga ctgataaaca aaaacaacga cccgctaaca
     3061 atgtatataa aaaataggcg aaacagtagt aaattcaagg cttttggctc gtattatact
     3121 ttgtgcttaa ccgaaaattt ggcacttcga aatcgcctac ttttcgctga atttacaatc
     3181 caagtgcaac aaaaaaagaa gtactcatca actagaatat aattttgttt ccacacaaaa
     3241 accacttcca aatgatgagt acttcctacc gacatcttac aataagccga gtgtttgaga
     3301 aataccattt attaaactgt ctttttccat aagtgttgcc ataattctcc cccttgtttt
     3361 atctgctcat acccctgctt tgtcttttgc gttgagatat taccgtttca agctgtttat
     3421 taaactctgt aatgtcgtta attgctcgct gatttcgttg tactgttgaa tgatcaaggc
     3481 gttttcggct cgcaagctct ctaattcttt cgataatttc actcgctctg tttcttgctg
     3541 ttctagcaag gttttcaagg ttttctcgta aagtagttct agttcggtcg ccatttattt
     3601 tttcccctgt ttcttggttt cttggatttc gattactttc aggttctcgc cccacgttga
     3661 gttgataaaa tccttgtcca ctgctacaca aggctttatc cctttctccg ttttgcaagt
     3721 tgatagcttg atccccccac tctgctcttt ggctatctcg gctgaatttt gccattcggt
     3781 catctcttga taggcgttct tcgcctgcca cgtcaaaacg cctgcggtta tggctatcaa
     3841 ggctaatatc gtcaaggcga ttagggtcgg caagcgatac attctcacgg tattttttat
     3901 caatttcgcc tgctcgattt ggatattttc ggctaaattg ttccctgcgt gctgtaactg
     3961 ctgtatcaag ttttgctcgt gccgtttgat agcgttctgc actatctcgc ttgtactctc
     4021 tagctctttc tgtgatgtct gctgaaaggt ctgtgctaaa tctaaaatct tgctcataga
     4081 aagccccttt taatcttaaa ttctgtccgt cagtcttgat tgataggttt tttggcgtaa
     4141 tgcgtgcaat ttcaaaacct gcacgttcta gggcatttat aacgtcctgt cggtcgttta
     4201 tctgccctgc gttcgctaaa gctgttaaac cgtccgtaat cgcctgtaaa gcctgttttt
     4261 tatcggttgg taggtctttg cggtcaatca ggtttttttg ctttataggg ttgtttgggt
     4321 cgcttaggct gtattcgtgg ttgattacct gcttgaagtt ttcagcaagt gggcggtctg
     4381 ccctgtcgta gtagggttgt aggcgttttc cgcttgtcat ctctacgttt gggataacga
     4441 aattcagctc aagcctgcct ttgtcggtgt gttgtaccca cgcaatgtta tattgttcag
     4501 gctcaagccc tgcaaacatt gccttttcaa agcgtgccat aatctcacgc ttttgtattt
     4561 cgggtaggtc gctttcttca aaagacaagc aacctgcggt gtaagtgtta ttaaaatcaa
     4621 gactatcagc aatactttgt gatagtcggg gatcgccctg caggatttgg gcggtgtcgt
     4681 caggcttatt tagtaaatag tccacacaag ccttgcaact actagctttt cccttaccgt
     4741 gtttcttaaa aaatttaacg atcataggct caattctcgc aattttttga gatttttttc
     4801 aatttctcgc aaggtcgcca acacgctaac aaggtcaatg ctcggctttt gactgttgca
     4861 ttgtcgggcg atttggttca ggtttacgcc tatgcggttc agctcgaaca gtaacgcagg
     4921 gtcgattact ttcggctgtc gcttaggttg ctgttctagg gcaacttccc gaacccactc
     4981 cgcaagcctt gctttcgttt ttctctctaa caaggcttga tactcgtttt cggtgagcct
     5041 gatttttatc tctttctcac gtttcataat ggtgtccgtt cttctatttt gaccgctgaa
     5101 agcggtcggg ggtcaagggg gcaaagccct tttgcaaggt caaaaaggaa actcgaagag
     5161 tacccttttt gtgtccttgc tattaaaaaa gacaatagta caccaaaagc cccgagagtg
     5221 atatataaaa atcagcggag ccgataggcg acttcgt
//
LOCUS       DANLXS010000169         5214 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.170, whole genome shotgun sequence.
ACCESSION   DANLXS010000169 DANLXS010000000
VERSION     DANLXS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.170"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            62..208
                     /locus_tag="PXD71_002196"
     CDS             62..208
                     /locus_tag="PXD71_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292271.1"
                     /translation="MLAEKPKKRWRFWYAFVNGLFFDKKHCYAAYKAEVYRKQYPPEF
                     TEIT"
     gene            315..1496
                     /locus_tag="PXD71_002197"
     CDS             315..1496
                     /locus_tag="PXD71_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDL6292272.1"
                     /translation="MSILDTYLHGVEVVEVNAGGVTISTAATSVIGVVCTGDQADAET
                     FPLNTPVLITNPLNYLEKAGSTGTLRRTLNSIGSIVKTPTVIVRVAESDDSDTLTANI
                     VGTQENGKFTGIKALLTAQSTVFVKPKLLCVPQHDNQAVATELLSVAKKLNAFAFISD
                     NGATTKEQAYTYRQNFSQREGMMIFGDWKSYNTDKKAYDTDYAVARACALQAYIDKTV
                     GWHKNISNVELDGVTGITKAVEFDINESSTEANYLNEKGITICLNHNGFRYWGSRTLA
                     TDTRWAFQQSVRTAQIIKETIGAGLAWAVDMPLTPLRVKTMLEAINNKLRSWASGDDP
                     RILGARVWVAEEITADIIKSGKFVIKYDYHWIPSLESLGLEQRVNDEYVVDLVNTLKA
                     L"
     gene            1505..2011
                     /locus_tag="PXD71_002198"
     CDS             1505..2011
                     /locus_tag="PXD71_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL6292273.1"
                     /translation="MGLPAKLKNFNFSVDGTSYLGETTEVTQPKLAMQLEDYRAGGMI
                     APVGVNMGLEKLELEFKIGGHEEDLLKLFGGSISGNAFRFNGAYQHDDDDSVDAVELV
                     CRGRIAEIDEGSSKAGDDTEHSYKASLTYYKKTVNGVDIIEIDTLNQIYIVDGKDRLA
                     EIRKAMGL"
     gene            2090..2404
                     /locus_tag="PXD71_002199"
     CDS             2090..2404
                     /locus_tag="PXD71_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL6292274.1"
                     /translation="MKKVTLKQGILRGEKRITEIEVRKPLTKQLRGTNLTSLMQLSVD
                     EWCIVLPRITTPKLDKADFATMSAADLLKLSGKALDLMSEDFDEADEEDSEEQGKGEI
                     LA"
     gene            2428..2544
                     /locus_tag="PXD71_002200"
     CDS             2428..2544
                     /locus_tag="PXD71_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL6292275.1"
                     /translation="MADIATVFHWTPNTFDEMTIVELGQWREKARLRNGVES"
     gene            2608..2955
                     /locus_tag="PXD71_002201"
     CDS             2608..2955
                     /locus_tag="PXD71_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292276.1"
                     /translation="MEIKTVTFSKVITAFIFHIITLFVFTQLNEQAQATFMFFWWFFI
                     SLRFILVVGIMCVGIEELKIQEYRQTKAYACFSFISNFALCLQFVYFEHLIAAILFLI
                     SYIIYHSQTVTIK"
     gene            2957..3394
                     /locus_tag="PXD71_002202"
     CDS             2957..3394
                     /locus_tag="PXD71_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292277.1"
                     /translation="MLQQFAMMSLGYFVFMRLTIPYQDTSREMNWSHVQNDVVGAMPK
                     SQFTGKAGETMEISAELRPEITGGKMSILALEMMAEQGSAYPLISGSTFMVLGWFVID
                     RISEQETTFFADGTPRAISFSMSLKRVDDSLLANIIDEVAGFI"
     gene            3394..4632
                     /locus_tag="PXD71_002203"
     CDS             3394..4632
                     /locus_tag="PXD71_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDL6292278.1"
                     /translation="MDFLTELTNHNHRTPAVSVTVRPKPSKDNEGEKAKDISTLITHR
                     LIQLTLTDNRGFEADQLDLELDDTDGLLALPSRGAILSVGLGWQNSPLTYKGEYTVDE
                     LTHDGPPDKVTIRARSADLRGTLTNRHERSFHRTTIGKIVKQIAEENKLKPMVGKEFE
                     NEEVKHIDQTNESSINLLQRLAEQFDAIATVKNGNLIFIKAGNATTASGKLLPLFRIT
                     RSSGDSHSFSIAEGDNYKAVKAYWHNTQTGKRGEVTWDENSQVKKVTKPTMRKKTKVK
                     RGADGKPIKGKDGKSIKETVFVKGKGRQVNAVVQSKPIESDSEAIKTLPHTYATAQSA
                     INACKRNFEKLQRGVATFSLTLAEGNAELIPELPVQVSGFKAEIDSNEWIISQVTHSL
                     NKGGGFTTALEMELKPKSED"
     gene            complement(4815..>5214)
                     /locus_tag="PXD71_002204"
     CDS             complement(4815..>5214)
                     /locus_tag="PXD71_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292279.1"
                     /translation="LPFKYNKADEAMHIIANNIIRKKTAVANAILALNEVDSRKSSGV
                     QLCDLLLGAILSSYQNDITSERKKELTKWIALHLGWDKLTYDTLQEEKKFNIWYFYDP
                     TKGPRIVRTRETKLKYPLLKKKEQTFQPAR"
BASE COUNT         1547 a         1101 c         1187 g         1379 t
ORIGIN      
        1 gtgtagcgca atttaaacaa gctccgctac cttttccagc agccgttgtt accgtggtgc
       61 aatgcttgcg gaaaagccga aaaagcgttg gcgtttttgg tatgcgtttg tgaatggctt
      121 gttttttgat aaaaagcatt gctatgcagc ctataaagcg gaggtgtatc gaaaacagta
      181 tccaccggag tttaccgaaa tcacttaatc ctgttaaacc ccatttcaca gcccgaaccg
      241 ctcgcaaccc gagcggttat tttttaaagt ttaccccaac attcaaccct aacctttccc
      301 aattaaggaa tactatgtca attttagata cctatctaca cggtgttgag gtggttgagg
      361 tcaatgcagg tggtgtcacc atttccaccg ctgctacttc ggttatcgga gtagtttgca
      421 ccggagacca agccgatgcc gagacttttc cgctgaatac gcccgtttta attactaacc
      481 cgttaaatta ccttgaaaaa gcaggtagca ccggcacgtt acgccgcaca cttaattcga
      541 ttggctcaat cgttaaaacg ccaacggtga tcgtgcgagt tgccgagtcg gacgatagcg
      601 acaccttaac tgcaaatatt gtcggcacgc aggaaaacgg caaatttacc ggtattaagg
      661 cgttattaac cgctcaatca accgtgtttg ttaagccgaa attgctttgt gtaccacagc
      721 acgataatca agccgttgcg accgaactat taagcgtggc taaaaagctg aatgcgtttg
      781 cgtttatttc agacaacggt gcaaccacca aagaacaggc atatacctat cgccagaatt
      841 tctctcagcg tgaaggaatg atgatcttcg gtgactggaa atcttataac actgataaaa
      901 aagcctatga taccgattat gcagtggccc gagcttgtgc gttgcaagcc tatatcgaca
      961 aaactgtcgg ctggcataaa aacatctcaa acgtagagct agacggtgta accggtatca
     1021 ctaaagcggt agagtttgat attaacgaga gctcaaccga ggcaaactac ctgaacgaaa
     1081 aaggcattac catttgctta aaccataacg gtttccgcta ttggggttcc cgcactcttg
     1141 ccaccgatac ccgctgggcg tttcagcagt cggtgcggac ggctcaaatc attaaagaga
     1201 caatcggagc aggcttagct tgggcggtgg atatgccact cacaccgttg cgtgtgaaaa
     1261 caatgctaga ggcgattaac aacaagctac gctcttgggc atcgggtgat gaccctcgca
     1321 ttttaggtgc tcgtgtgtgg gtagctgaag agatcaccgc agatattatc aaatcaggca
     1381 aatttgttat taaatacgat taccattgga ttccgtccct cgaaagccta ggcttagagc
     1441 aacgggtcaa tgatgaatat gtagtggatt tagtcaatac acttaaagcg ttatagaggt
     1501 aaaaatggga ttaccggcaa aacttaagaa ttttaatttt tcggtggacg gcacaagcta
     1561 tttgggcgaa accaccgaag tcacacagcc gaaactggct atgcaacttg aagactaccg
     1621 agcaggcgga atgattgccc ctgtgggtgt caatatgggt ttagagaaac tggagcttga
     1681 gttcaaaatc ggcggacacg aagaggattt gctcaagcta tttggtggct caatcagtgg
     1741 taatgcgttc cgtttcaacg gtgcgtacca gcacgacgat gacgattcag tcgatgcggt
     1801 agagttagtc tgccgtgggc gaatcgctga aattgacgaa ggctcaagca aagccggtga
     1861 tgacaccgag catagctata aagcctcgct aacctattac aaaaaaacgg tgaacggtgt
     1921 cgatattatc gaaatcgaca ccctcaacca aatttacatt gtcgatggca aagaccgctt
     1981 agcggaaatt cgtaaggcaa tgggtttata gattttcccc taaattttcc caaagcccct
     2041 tccccttttt tcaaggggct tttaatgata aataattaag gattaaacaa tgaaaaaagt
     2101 aacattaaaa caaggcattt tgcgtggtga aaaacgtatt accgagattg aggtgcgtaa
     2161 gccattaacc aaacaactgc gtggcacaaa tcttacctca ttaatgcagt taagcgttga
     2221 tgagtggtgt attgtactgc cacgcattac cacgccaaaa ttagacaaag ccgattttgc
     2281 cacaatgtcg gcagcggatt tgctaaaact ctccggcaaa gccttagatt taatgagcga
     2341 agacttcgat gaggcagacg aagaggacag cgaagaacag ggaaagggcg agattttagc
     2401 ctaattccac agtttgttga tgacgctatt gctgatattg ctaccgtgtt tcattggaca
     2461 cccaacacct ttgatgaaat gacgattgtc gagctagggc aatggcgaga aaaggcccgc
     2521 ttacgaaatg gggtggaaag ttaaaacaag cggtcgtttt tcgcaaaaaa tttgcaaaac
     2581 tcgaccgctt gctataagga ttttaaaatg gaaattaaaa cagttacatt cagtaaagtg
     2641 attacagcat tcatttttca tatcattacg ttatttgttt ttactcaact taatgagcaa
     2701 gctcaagcta cctttatgtt cttttggtgg ttcttcattt ctctgcgatt catccttgta
     2761 gttggaataa tgtgtgttgg tattgaagaa cttaaaattc aggaataccg acaaacaaaa
     2821 gcttatgctt gttttagttt catatccaat tttgcgttat gcttgcagtt tgtttacttt
     2881 gagcatctta ttgcggcaat tttatttttg atttcttaca tcatatatca tagccaaact
     2941 gtaacgatta aataatatgc tccaacaatt cgcaatgatg tcgctaggct attttgtgtt
     3001 tatgcgattg acaatccctt atcaagacac cagccgagaa atgaactggt cgcacgttca
     3061 aaatgatgtg gttggggcaa tgccaaaaag ccaatttacc ggcaaggcag gggagacaat
     3121 ggagatttca gccgagctac gccctgaaat taccgggggc aaaatgtcaa tccttgccct
     3181 tgagatgatg gcagagcaag gctcggctta tccgttgatt agtggttcaa cctttatggt
     3241 gctaggctgg tttgtgattg atcgaatcag cgagcaggaa accacctttt ttgcagacgg
     3301 cacgccccgg gcaattagct tttcgatgtc gcttaaacgg gttgatgata gtctgctcgc
     3361 caatattatt gatgaggtcg cagggtttat ttaatggatt ttttaacaga gctaaccaat
     3421 cataaccacc gcacaccggc tgtttcggtg acggttcgcc caaagccaag caaagataat
     3481 gagggcgaaa aagccaaaga tatttccacc ctgatcacgc accgcttaat tcaactcacc
     3541 ttaaccgaca accgaggctt tgaggcagac cagctggatt tagagctgga cgacactgat
     3601 ggcttactcg ctttgccaag tcgaggagcg attttatcgg tcgggttagg ctggcaaaac
     3661 agccccctga cctacaaggg cgaatacacg gtggacgagc taacccacga tggcccacct
     3721 gataaagtca cgattcgggc aagaagtgct gatttgcgag gcacgctgac caatcgccac
     3781 gagcggagct ttcaccgcac caccattggt aaaattgtga aacagatcgc cgaagagaac
     3841 aaactcaagc cgatggtggg caaggagttt gaaaacgaag aggtgaaaca cattgaccaa
     3901 acaaacgaaa gctctattaa tctcttgcaa cgcctcgcag aacaatttga tgccattgct
     3961 accgtgaaaa acggcaattt gattttcatt aaagccggaa acgccacaac cgcaagcggt
     4021 aaactgttac ccctgtttcg cattacccgc agttcggggg attcccatag cttttcgatt
     4081 gccgaagggg ataactacaa agcggttaaa gcctattggc acaacacgca aaccggcaag
     4141 cgaggcgagg tgacgtggga cgaaaacagc caagttaaaa aagtgactaa gcccacaatg
     4201 cggaaaaaga ctaaagtaaa acgtggggca gacggcaagc cgataaaagg caaagacggc
     4261 aagagcatta aagagaccgt ctttgtaaaa ggcaaaggca ggcaagttaa tgcagtggtg
     4321 caaagtaaac cgattgaaag tgatagcgag gcaattaaaa ccctacccca tacttacgct
     4381 accgcacaat cagcgattaa tgcttgtaaa cggaattttg aaaaactaca acgaggtgtg
     4441 gcaactttta gcctaactct tgccgagggc aatgcggagc ttatccccga attaccggtt
     4501 caggtttcag gctttaaggc ggagattgat agcaacgagt ggataatttc gcaggttacg
     4561 cacagtttaa ataaaggtgg tgggtttacg acagcgttag agatggagtt gaagccgaag
     4621 tctgaagact aacgctattt ttataaaatc actataaaaa agccttgcat ctctgatctt
     4681 gaacaagtaa tattaatcaa agttacagat ttatttacat aaaacacaaa gaatcatagg
     4741 ggcggctact ttgtgtgatt ccgcccagtg aggaaattgg gcgttgtttt acacatataa
     4801 gtccttgagg acactcaccg tgcaggctgg aaggtctgct ctttcttttt taatagtggg
     4861 tatttaagtt ttgtttctct cgttcttact attctaggtc cttttgtagg gtcgtaaaaa
     4921 taccaaatat taaatttctt ttcctcctgt aaagtatcat aagttaattt atcccaacct
     4981 aggtgcaaag caatccattt tgttagttct tttttacgtt ccgatgtaat atcattttga
     5041 tagctactta atattgctcc taataataaa tcgcatagtt gaacgccaga tgatttacga
     5101 gaatctactt cgtttaatgc tagaatagca tttgctacgg cagtcttttt acgaattata
     5161 ttattagcga taatgtgcat tgcctcatca gctttattgt atttaaaagg taga
//
LOCUS       DANLXS010000170         5078 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.171, whole genome shotgun sequence.
ACCESSION   DANLXS010000170 DANLXS010000000
VERSION     DANLXS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.171"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..114
                     /locus_tag="PXD71_002205"
     CDS             <1..114
                     /locus_tag="PXD71_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HDL6292280.1"
                     /translation="PKAHFELYLKECEWRFNHSDLKSQIFLLKQLVKGGLG"
     gene            complement(115..1002)
                     /locus_tag="PXD71_002206"
     CDS             complement(115..1002)
                     /locus_tag="PXD71_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide transport protein"
                     /protein_id="HDL6292281.1"
                     /translation="MNKLTQLALISLAFTGSAFAAPKSFTYCLESSPAALNPQIATDG
                     ATLDVGQAIYNRLIDFEKGTTNLTPSLAERWEISEGGKIYTFFLRQNVQFQSNKSFTP
                     SRYFNADDVIFSINRQFDKTNPYHKLSGGNYEFFYGMNMQNIIKNVTKVDDYTVKIEL
                     SEPNAPFLANLAMDFMAIFSAEYANKMQIAGTPEKLDLEPIGTGAFQFYGYQKDAAVR
                     FTAFEHYWEGKAKIDRLVFSITPDASVRLAKLEKNECQAMPYPNPSDLSALKANKDIQ
                     LFEKSGLNIGYITFRGCPR"
     misc_feature    complement(1028..1173)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(1350..2360)
                     /gene="mglC"
                     /locus_tag="PXD71_002207"
     CDS             complement(1350..2360)
                     /gene="mglC"
                     /locus_tag="PXD71_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDL6292282.1"
                     /translation="MSALQQNRTLDFFKQNAIYFVLLILLIVIIVQDPSFLSLRNFSN
                     ILSQSSVRLIIALGVAGLIVTQGTDLSAGRQVGLAAVISATLLQSMENMNKVFPNLSE
                     IPIPVVVLTVCAIGAVIGLFNGFVVAYLNVTPFIATMGTMIIVYGINSLYYDAVGSSP
                     IAGFSDDFSTFAQGFFRFGDFRLSYITIYAVIATIIMWTLWNKTRFGKNVFAIGGNPE
                     AARVSGVNVPLNLMGIYMLSGIFYAFGGMLEAGRIGSATNNLGFMYEMDAIAACVVGG
                     VSFAGGVGTIIGVVTGVLIFTVINYGLTYIGVNPYWQYIIKGGIIILAVAIDSLKYAK
                     KK"
     gene            complement(2374..3873)
                     /gene="mglA"
                     /locus_tag="PXD71_002208"
     CDS             complement(2374..3873)
                     /gene="mglA"
                     /locus_tag="PXD71_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDL6292283.1"
                     /translation="MTGEVLLTMTNVSKSFPGVKALDNANLTVKSHSVHALMGENGAG
                     KSTLLKCLFGIYAKDEGDILFLGKPVNFKTSKEALENGISMVHQELNLVKQVTVMDNL
                     WLGRYPLKGPFVDHDKMYRDTKAIFDELDIDVDPRDKVAKLSVSQMQMIEIAKAFSYN
                     AKIVIMDEPTSSLSEKEVEHLFKIIQKLKDRGCGIIYISHKMDEIFKICDEITILRDG
                     KWVNTVAIKDTSMDQVVSMMVGRELTQRFPEKTNLPKEVILEVEHLTAKNQPSIQDVS
                     FSLRRGEILGIAGLVGAKRTDIVETIFGVREKAGGTIRLNDKEINNRNAFDAINNGFA
                     LVTEERRSTGIYANLSIEFNSLISNMKSYLGKFGLLSNKKMKSDTQWVIDSMNVKTPS
                     HQTHIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDIGAKFEIYQLILELANKDK
                     GIIMISSEMPELLGVTDRILVMSNGKVAGIVETAHTSQEEILQLAAKYL"
     gene            complement(3945..4934)
                     /gene="mglB"
                     /locus_tag="PXD71_002209"
     CDS             complement(3945..4934)
                     /gene="mglB"
                     /locus_tag="PXD71_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL6292284.1"
                     /translation="MKKAVLSAVAMAVALGATISTAQAENRIGVTIYKYDDNFMALMR
                     KEIEKEGKTQKVELLMNDSQNTQSIQNDQVDVLLSKGVKALAINLVDPSAYKTIIQKA
                     KSEDVPVVFFNKDPGAAAIDSYPKAYYVGTEPKESGIIQGELIAKQWKAHPELDLNKD
                     GKIQFALLKGEPGHPDAEARTKYVVESLKAQGIDSEQIFMDAALWDAAQAKDKVDAWL
                     SSGKAKDIEVIISNNDGMALGALEATKAHGRKLPIFGVDALPEALQLIKKGELAGTVL
                     NDGVNQGKAVVQLSANLADGKDAVAGTQWKLENRVVRIPYVGVDKDNLSEFLK"
BASE COUNT         1487 a         1078 c          918 g         1595 t
ORIGIN      
        1 ccgaaagccc attttgagct atatttgaaa gaatgcgaat ggcgttttaa tcacagtgac
       61 ttaaaatctc aaatattcct tttaaaacaa ttggttaaag gggggttagg ttagttatct
      121 aggacagccc ctaaaagtaa tataaccgat atttaggcct gacttttcaa ataattgaat
      181 atctttattc gcttttaaag cagacaaatc tgacgggttc ggataaggca tcgcttggca
      241 ttcgttcttc tccaatttag ctaaacgtac tgaggcatcg ggcgtaatac taaatacaag
      301 gcgatctatc ttcgcttttc cttcccaata gtgttcaaat gctgtaaatc ttaccgctgc
      361 gtccttttgg taaccataga actggaatgc acctgtgcct ataggttcta aatctaattt
      421 ttccggcgta cccgctattt gcattttatt tgcatattcc gcagaaaaga tcgccataaa
      481 atccatagct aaattagcaa gaaagggggc attcggttca gataattcga tttttaccgt
      541 gtaatcatct acttttgtta catttttgat gatgttttgc atattcattc cgtagaaaaa
      601 ctcatagtta ccgccggaca atttgtgata tggattcgtc ttatcaaact gacggttaat
      661 cgaaaaaata acatcatcag cattaaaata acgactaggg gtaaaacttt tattactctg
      721 gaactgcaca ttctgacgca agaaaaaagt ataaattttt ccgccttcac tgatttccca
      781 tcgctctgcc agagaaggag tgaggttagt ggtacctttt tcaaaatcaa tcaggcgatt
      841 atagattgct tgcccgacat caagagtagc tccatctgtt gcaatctgag gattcaatgc
      901 tgccggagat gattccaaac agtaagtaaa agatttaggt gctgcgaaag cagaacctgt
      961 aaatgctaaa gaaataagtg caagttgtgt cagcttgttc attgtcatat ccttaaatgt
     1021 taagcaataa aaaacccttt caaaagttgc cttccgaaag ggttagaatt gggtttgcga
     1081 ttttggattg cgtttgccat ttcaggtttt cggaagatat cttccgaaca cgccaaatgc
     1141 ccgaaagatt aatcggtcaa atggtagtga tgatgtttaa cgcaatagct cataatattc
     1201 tccaaaattg aatgtttagg aatttacgct ttttaaaaat caattgcaaa cgttttcttt
     1261 aaaattttgt aaaaaattca acatttttca ccgcttgtat cgtacctagg agcaaatggc
     1321 gatttgctcc taggtttcca agcaataggc tattttttct tcgcatattt aagtgagtca
     1381 atcgctaccg ccaagataat gatgccacct ttaataatat attgccagta agggtttact
     1441 ccaatatagg ttaagccgta gttgataacg gtgaagatta acacccctgt tactacaccg
     1501 atgatagtac ccacaccacc ggcaaaggat accccaccta ccacacaagc ggcaatggca
     1561 tccatttcat acataaagcc taagttgttg gttgcactac cgatacgacc tgcttctaac
     1621 ataccgccga aggcatagaa gatgcctgaa agcatataaa tacccatcag gttaagcggt
     1681 acgtttacac cggaaactcg tgctgcttca gggttgccgc caatagcaaa tacgttttta
     1741 ccgaagcggg ttttattcca taacgtccac ataataatgg tagcaatcac ggcataaata
     1801 gtgatgtagg atagcctaaa gtcgccaaag cggaagaagc cttgtgcaaa ggttgagaaa
     1861 tcatcgctaa agcctgcaat tggcgatgag cctactgcat cgtagtaaag cgagttgata
     1921 ccatacacaa taatcattgt tcccatcgtg gcaataaacg gtgttacgtt gaggtaagcc
     1981 accacaaagc cgttaaataa accaatgaca gcaccgatgg cacacacggt taatacaacc
     2041 acaggaatcg ggatttccga gaggtttggg aataccttgt tcatattttc cattgattgg
     2101 agcaaggtgg cggaaattac cgctgccaag cccacttggc gaccggcaga taagtctgtc
     2161 ccttgtgtta caatcaagcc tgcaacccct aatgcaataa tcaaacgcac agaagattgc
     2221 gataaaatgt tactgaagtt acgcaagctc aagaaacttg ggtcttgcac aataatcaca
     2281 ataagtaaga ttaataatac gaagtaaatc gcattttgtt taaaaaaatc gagtgttcta
     2341 ttttgttgta aagcagacat acttcactcc ttgttataaa tattttgctg cgagttgcaa
     2401 gatttcttct tgcgaggtgt gagcggtttc gacaatacct gcaactttcc cgttactcat
     2461 cactaaaatt cggtctgtga ccccaagcaa ttccggcatt tccgatgaaa tcataatgat
     2521 gcctttatct ttattcgcca gctctaaaat caactggtaa atttcaaatt ttgccccgat
     2581 atcgatacca cgggtcggct cgtcaagcat caagatttca ggctgggtta acaaccaacg
     2641 cccgataatc actttttgtt ggttaccgcc ggaaagtgag ccaatatgcg tttgatgcga
     2701 tggggttttt acattcatcg aatcaattac ccattgggta tcgcttttca tttttttatt
     2761 gctcagtaag ccgaacttgc ctagataaga cttcatattt gaaatcagag agttaaactc
     2821 aatgcttaag ttggcataaa tccctgttga acggcgttct tctgtaacca atgcaaagcc
     2881 gttattaatc gcatcaaaag cgttgcggtt attgatttct ttatcgttta gtcggatagt
     2941 gccgcctgct ttctcccgta caccgaaaat ggtttcaaca atatcagtcc gttttgctcc
     3001 aactaagccg gcgatgccta aaatttcccc tctgcgtaag ctaaagctga catcttgaat
     3061 agagggttga ttttttgccg ttaaatgctc aacttcaaga atcacttctt tcggcagatt
     3121 ggttttttca gggaaacgct gggtcagctc acgccctacc atcattgaaa ccacttgatc
     3181 cattgaggta tctttaattg caacggtgtt tacccattta ccgtcacgca aaatggtaat
     3241 ttcatcacaa attttaaaaa tttcgtccat tttgtgtgaa atatagataa tgccacagcc
     3301 tctgtctttc agtttttgga taattttaaa taaatgctcg acttcttttt ctgaaagtga
     3361 cgaagttggt tcgtccataa tcacaatttt ggcattgtaa gagaaagctt ttgcaatctc
     3421 gatcatctgc atttgtgaaa ccgataattt ggctacttta tcacgtggat caacatcaat
     3481 atctaattca tcaaaaatcg ccttagtatc acgatacatt ttatcgtgat ctacaaaagg
     3541 cccttttagt gggtaacgac ctagccataa gttatccatt accgtcactt gttttactag
     3601 gttcaactct tggtgaacca ttgagatccc attttctaac gcctcttttg aggtcttaaa
     3661 attcaccggc ttgccaagaa agagaatatc tccctcatct ttagcataaa tcccaaataa
     3721 gcatttcagt aacgttgatt tacctgctcc attctctccc attaaggcgt gaacactatg
     3781 ggatttcacc gttaagttag cattatctaa ggcttttacc ccgggaaatg acttgctaac
     3841 atttgtcatt gtgagtaaaa cttcacctgt catatctcac ccctgattac atcgaaaaat
     3901 cgaaaaaaag cacgggtatc catcacccgt gctaaaagcg tgaattattt taaaaattca
     3961 gataagttgt ctttatcgac accaacgtaa ggaatacgca ccacacggtt ttccaatttc
     4021 cattgtgtac cagcaactgc atctttaccg tctgctaagt tagcagaaag ctgaacaacc
     4081 gctttacctt ggtttacacc atcatttaat accgtaccgg ctaattcacc ttttttgatt
     4141 aattgaagtg cttctggcaa tgcatctaca ccgaagattg gtaattttct accgtgagct
     4201 ttggttgctt ctaatgcacc taacgccata ccatcattat tagaaataat tacttcgatg
     4261 tctttcgctt taccacttga taaccaagca tccactttat ctttagcttg ggctgcatcc
     4321 cataatgctg cgtccataaa gatttgctca ctgtcgatac cttgtgcttt taagctttct
     4381 accacatatt tagtacgtgc ttccgcatca gggtgacccg gctcaccttt taataaggca
     4441 aattggattt taccgtcttt attcaagtct aattcaggat gggctttcca ctgttttgca
     4501 attaactcac cttgaataat gcctgactct ttcggctcag tacctacata ataagctttc
     4561 ggatagctat caatcgcagc tgcacccggg tctttattga agaacaccac aggcacatct
     4621 tcactttttg ctttttgaat aattgtttta taagctgatg gatccactaa gttaattgct
     4681 aatgctttaa cccctttaga aagtaataca tcaacttggt cgttttgaat agattgcgta
     4741 ttttgcgagt cattcattaa taactcgact ttttgtgttt taccctcttt ttcgatttct
     4801 ttacgcatta atgccataaa gttatcgtca tatttataaa ttgttacacc aatacggttt
     4861 tcagcttgag ctgtactgat cgttgcacct aatgcgactg ccattgccac tgcacttaat
     4921 actgctttct tcatagtaga tcacctcttt tatgtgttta tagggaaatg aattgcttac
     4981 cataagcaac ttggtgcaaa gtataactaa ttttaaaaaa acaatccgtg atcattctca
     5041 cagaattaag acaaagtgga gaaattttta attttaag
//
LOCUS       DANLXS010000171         4983 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.172, whole genome shotgun sequence.
ACCESSION   DANLXS010000171 DANLXS010000000
VERSION     DANLXS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.172"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..151
                     /locus_tag="PXD71_002210"
     CDS             <1..151
                     /locus_tag="PXD71_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL6292285.1"
                     /translation="SVFNELLEEPFLLSEYDAYQFTNEVIKFHSEESLKAWGGCLIWE
                     QNNGS"
     gene            283..981
                     /gene="aphA"
                     /locus_tag="PXD71_002211"
     CDS             283..981
                     /gene="aphA"
                     /locus_tag="PXD71_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDL6292286.1"
                     /translation="MTAATMFASLIAFSAQAGGPKVAYTQPGIDMRTHEAQQAPIHWV
                     SIEQIKESIKDLPPMAVSFDIDDTVAATSGCFHYGKQKYSPDDFSYLKNQDFWDEINA
                     GCDKYSLPKQVAINLIKMHQERGDQVYLITGRTAGKNEQVTTIMQKYLGIKNMQPVNF
                     MGGKAHSTKYDKTPAIIEHKVQIHYGDSDDDILAAREAGIRGIRILRAANSNYTPFPQ
                     AGGYGEEVVVNSSY"
     gene            1162..1923
                     /gene="udp"
                     /locus_tag="PXD71_002212"
     CDS             1162..1923
                     /gene="udp"
                     /locus_tag="PXD71_002212"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDL6292287.1"
                     /translation="MSEVFHLGLTKAMLKGAKIAIVPGDPARSERIAKKMDNPEFLAS
                     TREFTSWLGYLDGEPVVVCSTGIGGPSVSICVEELAQLGVRTFLRIGTTGAIQEHINV
                     GDILITTGAVRLDGASQHFAPMEYPAVANFECTNALYAAAVESGVTPYVGITASSDTF
                     YPGQERYDTFTGKIYRHFQGSLKQWQDLNVMNFEMESATLFTMCSALGLRAGMVAGVI
                     VNRTQQEIPNEATIHDTEHKAVSVVVEAARKLIKG"
     gene            1998..2583
                     /locus_tag="PXD71_002213"
                     /pseudo
     CDS             1998..2583
                     /locus_tag="PXD71_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2594..3079
                     /locus_tag="PXD71_002214"
     CDS             2594..3079
                     /locus_tag="PXD71_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDL6292288.1"
                     /translation="MYSLYLKILSECDWCCDPKTLSTYQQEWLLHEGSLTQKLYDVAT
                     NFNVEVVQEKWIAKNSEKTTACNEDVWSREVLLKDGEKNWIFAQTLVPRSTIENIAQN
                     VLELGNQPIGLWLFSQNPTRTKLEWTQTENGLFARRGVYEIRGYSLEIRELFLETFPY
                     L"
     gene            complement(3117..3986)
                     /locus_tag="PXD71_002215"
     CDS             complement(3117..3986)
                     /locus_tag="PXD71_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL6292289.1"
                     /translation="MKNINTQAVGWIGLGQMGEPMAKHLLNSGVNVGVYNRDPAKAEK
                     VVEAGGTLYPSVLELVKAYDVIFLMIADYAAVQQVLSDEVLAELNGKVVVNMSTISPS
                     QNQAVEALLAQHGAEFVEAPVSGSSKVAEAGKLLVLAAGKEETVELLKPLFAAFSTTT
                     FYYGEVGKAGGIKLMINSLLGIFIQAYGEALNFADQYGIPKEKVIEMISGSFMNSPIF
                     QAKVPMYQANEFPPAFMMKHMTKDFNLASEEIAKLGKSYPLIEQATKTYNQANDSGLS
                     EVDMAAIYQFLAK"
     gene            4116..4907
                     /locus_tag="PXD71_002216"
     CDS             4116..4907
                     /locus_tag="PXD71_002216"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL6292290.1"
                     /translation="MKPTILLYDSGMGGLTIYDEIRKVLPHAHYLYCFDNAFFPYSEK
                     SEAVLIERAVKIVQKIAEKQPLDMVVVACNTASTVVLPALREHFNFPIVGTVPAIKPA
                     AAISETKVIGLLATKGTVARPYVDELIRKYAQDCTVEKIGTTDLVEIVEEKQRSGKVD
                     MNRLQKVVEAWQGKPELDTVILGCTHFPLVKAELQQLLPDVKYFVDPGVGIAKRVLSL
                     LEERKFQQKADFENQAFCTKQSSAFNIKQDVMQNWGFNNLSVIDI"
BASE COUNT         1544 a          901 c         1046 g         1492 t
ORIGIN      
        1 atcagtattt aatgaattat tagaggagcc atttttatta agcgaatatg atgcctatca
       61 atttacgaat gaggtgatta aatttcattc agaagaaagc cttaaagctt ggggaggttg
      121 cttgatttgg gaacagaaca atggctcata agcgttaagc gtaatcgttt gcttgtgatc
      181 atattcacaa aataaggttt ttgtattagc taagagtgcc taataagatt aaaataggta
      241 tggttaatca tatggaggta ttttataaaa tttacattaa aaatgacggc agcgacaatg
      301 tttgcatctc ttattgcttt ttcagcacaa gcaggcggac caaaagtagc ttatacccag
      361 ccgggaattg atatgcgaac acatgaagca cagcaagcgc caattcattg ggtatctatt
      421 gagcaaatta aagaaagcat caaagattta ccccctatgg cggtaagttt tgatattgat
      481 gatactgttg ctgcaactag cggttgcttc cactacggaa agcaaaaata ttcgccagac
      541 gatttcagct acttaaaaaa tcaagatttt tgggacgaaa ttaatgctgg ctgtgataaa
      601 tattctttgc caaagcaagt tgctattaat ttaataaaaa tgcaccaaga acgtggtgat
      661 caagtttatt taattacggg gcgtaccgca ggtaaaaatg aacaagttac aacgattatg
      721 caaaagtatc ttggtattaa aaatatgcaa ccggtaaatt ttatgggggg gaaagcacat
      781 tcaacaaaat atgataaaac ccctgcgatt attgaacata aggtgcaaat tcactatggt
      841 gacagtgatg atgatatctt agctgcccgt gaagccggta ttcgaggtat tcgtatactt
      901 cgtgcagcaa attctaatta tacgccattc cctcaggccg gaggatatgg tgaagaagtt
      961 gttgtcaatt caagttatta atttcttagt ctgaaatatg gccaccattg cgtggccatc
     1021 attttttatt tatataatga cgaaaccgtt tacctttgat ctagttcaca aaatcaaact
     1081 ttgcttgcaa aaaattgtcg taaattaacc gcttgctaag atagaatggc tcatatccag
     1141 ttagataaaa aggagataaa aatgtctgaa gtatttcatt taggtttaac caaagccatg
     1201 ctcaaaggtg caaaaattgc aatcgttccg ggcgatccgg ctagaagtga acgtattgcc
     1261 aaaaaaatgg acaacccaga gtttttagcc tcaacccgtg aatttacctc ttggttaggt
     1321 tatttagatg gcgaaccggt ggtggtttgt tcaaccggta ttggcggacc gtcggtgtcg
     1381 atttgtgtgg aagagttagc acagttaggt gttcgcacat tcttacgcat tggcacaaca
     1441 ggtgcaattc aagagcatat taatgtaggc gatattttaa ttaccaccgg agcggtgcgt
     1501 ttagatggtg cgagccaaca ttttgcacca atggaatatc ctgcagtagc gaattttgaa
     1561 tgtactaatg ctctttatgc agcagcagtg gaatcaggcg ttacacctta tgtggggatt
     1621 acagcttcat cagacacttt ctacccgggg caagaacgtt acgacacttt caccggcaag
     1681 atctatcgtc atttccaagg ttcattaaaa cagtggcaag atctcaatgt tatgaatttt
     1741 gaaatggaat ccgccactct ctttacgatg tgttcggcat taggcttgcg agcaggtatg
     1801 gttgctggtg taattgtaaa ccgcactcaa caagagattc cgaatgaagc aaccattcac
     1861 gacaccgaac ataaagccgt ttcggtagta gtggaagcgg caagaaaatt aattaaaggc
     1921 taatctttaa atttaaaagc aaaaaaggta aattgtcttt gttacaattt accttttttc
     1981 tttttaggga gctttctatg aaaaaatggt tgatgttaat tacattaatt ttcggcatta
     2041 ccgcttgttt ttccagttta gaatttgatg aaagtaaaca agaaaaaatc aattcattct
     2101 tgattgttgg taataaatta tatgtggtgg gtgaactgca cgattatgaa tttgagcatc
     2161 agcaagttgc ggaactgaat aattttctca aaagcctaat tgctaataaa atcaaatctg
     2221 ttgaggcaaa cttcgaggtt aaggaatcca aaaaagtgga aggtagttat agaattattt
     2281 tagatgggaa atcacttaca gaaagtgaaa gaagtacggt gatggtttaa ttcagctcaa
     2341 aatccatcta ttacttatca ggcggaaggg aacttggtga aattagccaa tagagaagag
     2401 cttttgcaaa aattccgatt taaacaagcg cttgaggcga atgtgcgtta ttacaaaatg
     2461 aaaagtggtc ttcaaaatac ggctgaagga gcgttaagta ttgtaatgtt gcctgttgct
     2521 gtagtagctg ttttaccatt ctatatgttt tgggttggtg cttgtgccac ttacggatgt
     2581 taaataactt tttatgtatt ctctatacct gaaaatttta tcggaatgcg attggtgttg
     2641 tgatccaaaa acactttcta cctaccagca agaatggctt ctgcacgaag ggtcacttac
     2701 ccaaaaattg tatgatgtcg caaccaattt caacgtggaa gtagtacaag aaaagtggat
     2761 tgcaaaaaac agtgaaaaaa cgaccgcttg taatgaagat gtttggtcgc gagaagtgtt
     2821 actgaaagat ggtgaaaaaa attggatttt tgcccaaact cttgtgccaa gatcaacaat
     2881 cgaaaatatc gctcaaaacg tgttggaatt gggcaatcag ccgattggtt tatggctatt
     2941 ttctcaaaat cctactcgca ccaaattaga atggacacaa acagagaacg gtttattcgc
     3001 tcggcgtggg gtgtatgaaa ttagagggta ttctctggaa attcgagagc tttttttaga
     3061 aacctttcct tatttataaa aaaatcggtt ggtaatgaca ccaaccgatt ttcatcttat
     3121 tttgccaaga actgataaat tgcagccata tccacttcgc tcaagcctga atcattggct
     3181 tgattgtagg ttttagtggc ttgctcaata agcgggtaag atttgcctaa tttcgcaatt
     3241 tcttcgcttg ccaaattaaa atctttggtc atatgtttca tcataaacgc aggcggaaat
     3301 tcattagctt ggtacatcgg cacttttgct tggaagatcg ggctgttcat aaacgagcct
     3361 gaaatcattt cgattacttt ctctttcgga atgccatatt ggtcggcgaa gtttaacgcc
     3421 tcgccatagg cttgaatgaa gatgcccagt agcgagttga tcatcaattt aatcccaccg
     3481 gctttaccca cttcaccata atagaaagtg gtggtactaa aggcagcgaa aagcggtttg
     3541 agcaactcaa cggtttcttc tttaccggca gccagtacca atagtttacc tgcttctgcc
     3601 actttgcttg aaccggaaac cggagcttca acaaattctg caccgtgttg ggctagtaga
     3661 gcttctaccg cctggttttg gcttggggaa atggtgctca tattcaccac tactttgccg
     3721 tttaattcag ccaacacttc atcgcttaac acctgttgca cggcggcata gtctgcaatc
     3781 atcaagaaaa tcacatcgta ggctttcacc aattccaaca cagaaggata taatgtgccg
     3841 cctgcttcaa ctaccttttc tgcttttgct gggtcgcggt tgtaaacgcc cacgttcact
     3901 ccgctattta gcaaatgttt tgccatcggt tcgcccattt gccctaaacc aatccaaccg
     3961 actgcttgtg tattaatgtt tttcattatt tttctcctta ctgaataaag caaattgatt
     4021 attgcgattt tccctcatca agtcaaatag agagaacttg aatatagcaa tggaaaagag
     4081 tagcatttta gcacttactt ttttgaacaa taccaatgaa accaacaatt cttttatacg
     4141 attccggaat gggcggattg acgatttacg atgaaatccg aaaagtgtta ccgcacgctc
     4201 actatttata ttgctttgat aacgcttttt tcccatattc cgagaagtcg gaagcagttt
     4261 taattgagcg agcggtcaaa attgtgcaaa aaattgcaga aaaacaaccg cttgatatgg
     4321 ttgttgttgc ttgtaatacg gcaagcacag tggttctgcc ggcattgcgt gagcatttta
     4381 attttccgat agtaggaact gtaccggcaa ttaaaccggc agcggcaatt tccgaaacta
     4441 aagtcatcgg cttgctcgcc actaaaggca cggttgctcg cccttatgtt gatgaattga
     4501 taagaaaata tgctcaggat tgtacggtag aaaaaattgg cacaaccgat ttggttgaaa
     4561 ttgttgaaga aaaacaacga agcggtaaag tggatatgaa ccgactacaa aaagtggtcg
     4621 aggcttggca aggtaagccg gaattggata cggttatact cggttgtacc cattttccct
     4681 tagtcaaagc tgaattacag caattattgc ccgatgtgaa atattttgtt gatccgggtg
     4741 taggtattgc caaacgggtg ctaagccttt tggaagaacg caaatttcaa caaaaagccg
     4801 attttgaaaa tcaagcattc tgcactaaac aaagttctgc atttaatatc aaacaagacg
     4861 ttatgcagaa ttggggattt aataatcttt cggtgattga tatttaaaca aaacaatctg
     4921 ttttgatgaa aaaaggttca gttgagcaaa aactttaaaa aagggcttgc actcaatttt
     4981 aaa
//
LOCUS       DANLXS010000172         4938 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.173, whole genome shotgun sequence.
ACCESSION   DANLXS010000172 DANLXS010000000
VERSION     DANLXS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.173"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(50..601)
                     /locus_tag="PXD71_002217"
     CDS             complement(50..601)
                     /locus_tag="PXD71_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292291.1"
                     /translation="MKGIMKSILVFSTAVFLAACSSNKSGWTRLNDNDVDQKSYAIAY
                     GATAQTYADRVNESYDINSFMNGVNDWYTKKVTLPIEQIRAITINRMLDHNVYAYYSG
                     VIYAADLQANFNHLDPNCWGLVQPASMTQGIHDAMLDLQKNKVRGDEYIKNGVEQILH
                     LCVKTIAEDENESQSKGKTKKHK"
     gene            694..1545
                     /locus_tag="PXD71_002218"
     CDS             694..1545
                     /locus_tag="PXD71_002218"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL6292292.1"
                     /translation="MKQYLDLCNRIVSEGKWVANERTGKHCLTVINADLTYDVEKGEF
                     PLVTTRRSYWKAAIAELLGYIRGYSNAADFRQLGTKSWDANANENAQWLANPYRKGED
                     DMGLVYGAVGRNFPKPDGGSVDLLRQIVDDLKKGIDNRGEIYTFYHPGAFHMGCLRPC
                     LHSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIV
                     NAHIYEDQLELMRDVQLKREPLNAPKLIINPDIKSLEDLETWVSLDDFKVEGYEYHPA
                     IQYPFSV"
     gene            complement(1599..2042)
                     /gene="lysM"
                     /locus_tag="PXD71_002219"
     CDS             complement(1599..2042)
                     /gene="lysM"
                     /locus_tag="PXD71_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL6292293.1"
                     /translation="MGLFDFVGDIGRKLFNKEEDASKAVTEHLAEDNPGVENVNVTVE
                     NGVAKISGIASTAAAVEKAVLMAGNVAGITEVNIDEVTLPDDVQLASDDELYVIQKGD
                     TLWEIATKAYGNGAKYKAIVEANKEVIKDENKIFPGQKIRIPKGL"
     gene            2281..2718
                     /locus_tag="PXD71_002220"
     CDS             2281..2718
                     /locus_tag="PXD71_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDL6292294.1"
                     /translation="MKQVQFYLLSQADAAELSAAEMQACELAAQAWRLGKRVLIACET
                     EQQALNIDEALWARDPDSFVPHNLSGEITTYPTPIEISWKGKRNAQRRDLLISLQTQL
                     PEFIGSFTQIIDFVPVEETAKAQARDRYKQYRQLGWQLETIQC"
     gene            2712..3155
                     /locus_tag="PXD71_002221"
     CDS             2712..3155
                     /locus_tag="PXD71_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6292295.1"
                     /translation="MLKNLSWRAKRFDELSTQELFEIYHARTAVFVVEQNCPYQEVDN
                     KDLQAVHFFTKNAKNLTAYCRLIPANDGVHIGRVLVVKENRGSGLARELVQKAMDYCG
                     EHFPAQPIHAQAQSYLQGFYESFGFKAVSEVYLEDGIAHLDMVLE"
     gene            3152..3893
                     /locus_tag="PXD71_002222"
                     /pseudo
     CDS             3152..3893
                     /locus_tag="PXD71_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            3895..4065
                     /locus_tag="PXD71_002223"
     CDS             3895..4065
                     /locus_tag="PXD71_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292296.1"
                     /translation="MTSREELLKKQRELDILFTAWFEEKKKHEVLTYRRENGDLIQHY
                     PDGTEKVIEYAQ"
     gene            4055..4648
                     /locus_tag="PXD71_002224"
     CDS             4055..4648
                     /locus_tag="PXD71_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292297.1"
                     /translation="MHNNLAIFYCGTNGAGKSTLRSFNQDKVEIVIDSDNIAMQINPL
                     NPRLADIEAGRKAIELFQFAVKNQVAFSMESTLSGKSILKRMENAKAGYFKTRLNYIG
                     VDSYKINIARVKARVKAGGHFIDEETIRKRYHISRENLIEAIFINDETLIYDNSETKP
                     NLILQIADKKVIKISDKIPNWCNQLCDELVELGFKTT"
BASE COUNT         1581 a          971 c          944 g         1442 t
ORIGIN      
        1 aaaaagcggt aaaatttttt aaaaatttta ccgcttgtca tagcttaact tatttatgct
       61 ttttggtttt acctttagat tgcgattcat tttcatcttc agcaatcgtt tttacacaaa
      121 ggtgcaatat ttgctcaacc ccatttttga tatactcatc tccacgaact ttgttcttct
      181 gtaaatctaa catcgcatcg tgaatgccct gggtcatact cgctggttga acaagccccc
      241 aacaattagg atctaaatga ttgaaatttg cctgcaaatc tgcggcataa atcacaccac
      301 tataataagc atacacatta tggtctaaca ttcgattaat cgttattgct ctaatttgct
      361 caattggcaa tgtaaccttt ttagtgtacc aatcatttac cccattcata aaggaattga
      421 tatcatagct ttcatttact ctatccgcat aagtctgagc cgttgcacca taagcaatag
      481 cataagattt ctgatcaaca tcattatcat ttaaacgtgt ccaaccgctt ttattcgatg
      541 aacaagctgc taaaaaaaca gcggtggaaa aaaccagaat ggatttcatt atacctttca
      601 tatccataac tccttattgt gttgtgtcat aaagttttag ctattttacc gaataaatgg
      661 caaaattacc taaaattttt tatgaggtaa ataatgaaac agtatttaga cttatgtaac
      721 cgcatcgtta gcgaaggcaa atgggttgca aatgaacgta caggcaaaca ctgcctcaca
      781 gtcattaatg ctgacttaac ttatgacgta gaaaaaggag aatttccttt agttaccact
      841 cgccgtagtt attggaaagc tgcgattgcc gaacttttag gctatattcg aggctatagt
      901 aatgctgctg atttccgtca attaggcaca aaatcgtggg acgctaatgc aaatgaaaat
      961 gcacaatggc ttgctaatcc ctatcgaaaa ggggaagatg atatgggctt ggtttacggc
     1021 gcagtagggc gtaatttccc gaaaccagac ggtggctcag tcgatttatt gcgacaaatt
     1081 gtggacgatt tgaaaaaagg aattgataac cgtggtgaaa tttatacctt ctaccacccc
     1141 ggcgctttcc atatgggctg cttacgtcct tgtttacaca gccatcattt ttctctactt
     1201 gatggcacgc tttacctgaa tagtactcag cgttcggcag atgtaccgct tgggttaaat
     1261 tggaatatga ttcaatgcta taccttttta gctttaatgg ctcaaattac agggcataag
     1321 gcaggacaag cgttccataa aattgtcaat gcgcatattt atgaagatca actagaatta
     1381 atgcgtgatg ttcaacttaa gcgagaacca ctcaatgcac ctaaattaat cattaatcct
     1441 gatataaaat ccttagaaga tttagaaact tgggtgagct tagatgattt caaagttgaa
     1501 ggttatgaat accaccctgc cattcaatat ccattttcag tctaacacca cctacaaaaa
     1561 gagctaaact ttcgaccagc ttagctcttt caatttgatt ataaaccttt cgggatacga
     1621 attttttgcc ctgggaagat tttattttcg tctttaataa cttctttatt tgcttctaca
     1681 atcgctttat attttgcacc gttaccataa gcttttgtgg cgatttccca caacgtatcc
     1741 cctttttgga taacataaag ctcatcatca cttgcaagtt gcacgtcatc aggcaaggtc
     1801 acttcatcaa tatttacctc agtgatacca gccacattac ccgccatcaa taccgctttc
     1861 tcaacagctg ctgcggttga agcaatgcct gaaattttcg ctaccccgtt ttcaaccgtt
     1921 acgtttacat tttcaacgcc cggattgtct tccgccaaat gctctgtaac cgcttttgaa
     1981 gcatcttctt ctttattaaa taatttacga ccaatgtcgc caacaaaatc aaataagccc
     2041 atagtaagtt ccttataaaa tagctaaatg aagtgggtat tctacacaat ttccaactat
     2101 tgaaatagtg cattgttctc ttagaacgtc taaaagctaa ttagttcctc aaggcaacgc
     2161 aacacaagtg gtcatttttt ctcaattttt tgcaaaaaca aacgcttaga ttttatattt
     2221 tactcatttt cagctagaat attaagttta aatatctgat tgaatttctg ggaacattct
     2281 gtgaaacaag tacaatttta tctattaagc caagccgatg cagccgagct ttccgctgct
     2341 gaaatgcaag cctgcgaact tgcggctcaa gcgtggcgat taggtaaacg ggtactgatt
     2401 gcatgcgaaa ccgagcaaca agcgttaaat attgacgaag cactttgggc aagagatccg
     2461 gattcgttcg tgccgcacaa tttatcggga gaaatcacca cttacccaac gcctatcgaa
     2521 atttcgtgga aaggtaaacg caatgctcaa cgtcgggatt tgttgatttc actccaaacc
     2581 caactccccg aatttatcgg cagttttacc cagattattg atttcgtgcc ggtggaagaa
     2641 accgccaaag cacaggctcg tgaccgctat aaacaatacc gccaacttgg ttggcaactg
     2701 gaaacgatac aatgctaaaa aacctctctt ggcgcgcaaa acgctttgat gaactctcca
     2761 cccaagagtt gtttgagatt taccacgcac gtacagcggt gtttgtggtt gagcaaaact
     2821 gcccttacca agaagtcgat aataaagacc tgcaagcggt gcatttcttt acaaaaaatg
     2881 caaaaaatct gaccgcttac tgccgtttaa ttccagccaa tgatggtgtg catataggta
     2941 gagtgttggt ggtgaaagaa aatcgtggct caggcttagc aagggaattg gtacaaaaag
     3001 cgatggatta ttgtggcgaa cattttcctg ctcaacccat tcacgcccaa gcccaaagtt
     3061 acctgcaagg gttttatgaa agttttgggt ttaaagcggt ttcagaagtg tatttggaag
     3121 atgggattgc gcatttggat atggtgttag aatgaataaa ttagctaaaa tcgaacaatt
     3181 aaaaacagca ctttcacaac gccgtccatt atctgaaagc gaagttcaac gactgcggga
     3241 agagtttatt attgaaagca gctataattc caatgcaatt gaaggcaata ccattacttt
     3301 gcgtgaaacg gtgctcattt taaaagaggg aatgaccatt gccgaaaaac cgattcggga
     3361 gcatttagat attatcggtt ttaaggatgc attcaactat atttatgaat tagtcgccaa
     3421 taaagaacca ttaagcgaac gagcgattaa agatattcat tctctggtat taatgggcaa
     3481 tgcggaaagc cgaggggttt atcgcaaaat tccagtacga attttagggg cagaaaatgc
     3541 ccccccctta tttaattgcc gaagaaatgc aattattgat tgaaaaatat caacaaaaac
     3601 tgaccgcact tcatcccata gaggcaatca gttggctgca tttagccttt gaaagcattc
     3661 acccttttat tgatggaaac gggctcacag ggcgattact gattaatttt gaattattaa
     3721 aacagggcta tttaccgatt gatattaaat ttaccaatcg agcccaatat taccaatgtt
     3781 ttgatgattt ccataaaaat caacaagata ttagtagttt ggttgaattg attagtcagt
     3841 atgaattagc tgagttggaa aggtatttgg cgattctgaa acagaggaca taaaatgaca
     3901 agtcgtgaag aattactgaa aaaacaacgt gaactggata ttctctttac agcatggttt
     3961 gaagaaaaga aaaagcacga agtgctgacg tatcgccgag aaaatgggga tttaattcag
     4021 cattatccgg acggtacgga aaaagtgatt gaatatgcac aataacctag caatttttta
     4081 ctgtggcaca aacggagccg gcaaatctac tttacgttct ttcaatcaag ataaagtcga
     4141 gattgtgatt gattccgata atattgcgat gcagattaac ccactcaatc caagattggc
     4201 cgatattgag gcgggcagaa aagcaatcga actatttcag tttgcagtaa aaaatcaggt
     4261 tgcattttca atggaatcaa ccctctcggg taaatctatt ctaaaacgaa tggaaaatgc
     4321 caaagcagga tatttcaaaa ctcgattaaa ttatattggt gtcgatagct ataaaattaa
     4381 catcgcacgg gtaaaagcaa gagtgaaagc cggcggacat tttattgatg aagaaaccat
     4441 tagaaaacgc tatcacatta gccgtgaaaa tttaattgaa gcgattttta tcaatgatga
     4501 aacactgatt tatgataatt cagaaaccaa gccgaatttg attttgcaga ttgctgataa
     4561 gaaagtgata aaaatttcag acaaaatacc gaattggtgt aatcagcttt gtgatgaatt
     4621 agtggaatta gggtttaaaa ccacctagca cgagccgtgc taggctcgac aatcccagct
     4681 cctttggagc tgtaacttag agccacaaag tggctcagct atgcgtaacc ccacacggct
     4741 tgtgtgtggg tgacaagctg tcggattaaa gaaaaacttt gcaaaaaact tctcgaaaca
     4801 gaccgcttat gcctatctct gataggttct taaataaata agctctcgtc gaagacgatc
     4861 accatcgggg atattggaac aaaaacacaa agatgaaaca gaataggaaa aagtctaaaa
     4921 aagttattgg tactcata
//
LOCUS       DANLXS010000173         4931 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.174, whole genome shotgun sequence.
ACCESSION   DANLXS010000173 DANLXS010000000
VERSION     DANLXS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.174"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..581
                     /locus_tag="PXD71_002225"
     CDS             <1..581
                     /locus_tag="PXD71_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL6292298.1"
                     /translation="TTIMQRISGAKQIDSLTVKIKDDIESQTAEKSIAELLVAKHGKK
                     DFFIMNSDTIKQTITSTTNTMTLLISSIALISLIVGGIGVMNIMLVSVTERTREIGVR
                     MAIGAKQRNILQQFLIEAVVICLIGGVIGILLASAIIWAFNTLGTNFKMMLSPESVII
                     AVLCSTLIGVVFGYIPARNAAQLNPITALAQE"
     gene            665..1435
                     /locus_tag="PXD71_002226"
     CDS             665..1435
                     /locus_tag="PXD71_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL6292299.1"
                     /translation="MTITNTELEQILNTKLNSSAINDYAPNGLQVEGKREIKKIITGV
                     TATLPLIEKAIEKNADAILVHHGYFWKSENPCIRGMKGKRIKQLLVNDINLFGYHLPL
                     DIHSELGNNAQLAKHLGVKNLQGLEDRPNSIPMYGELENPISANELKQKLEKTLQRTV
                     ILCDEFVHSPQKMIKTIGICSGGGQGYIDLAFEKGCDAFISGEISEQTTHSAREQGIY
                     YFACGHHATERDGVKALGEWLAVEYGLDVEFIDIDNPA"
     gene            complement(1516..2646)
                     /gene="nrdB"
                     /locus_tag="PXD71_002227"
     CDS             complement(1516..2646)
                     /gene="nrdB"
                     /locus_tag="PXD71_002227"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL6292300.1"
                     /translation="MAYTTFSQHKNDQLKEPMFFGQNVNVARYDQQKYEIFEKLIEKQ
                     LSFFWRPEEVDVSQDRIDYAQLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISAYYDD
                     LIRDSQLYSLYGEGSYQVDGKTCEVSLRSLKRQLYLCMMSVNALEAIRFYVSFACSFA
                     FAERQLMEGNAKIIKFIARDEALHLTGTQHILNIMAYGNDDPEMAEIARECRQEAYEL
                     FLSAAEQEKAWAEYLFKDGSMIGLNRDILVQYIEYITNIRMQAVGLPLPFKARSNPIP
                     WINAWLVSDNVQVAPQEVEVSSYLVGQIDAKVDSRDFGDFEL"
     gene            complement(2673..4928)
                     /gene="nrdA"
                     /locus_tag="PXD71_002228"
     CDS             complement(2673..4928)
                     /gene="nrdA"
                     /locus_tag="PXD71_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL6292301.1"
                     /translation="MVTKRNGTREQLNLDKIHRVITWAAEGLENVSVSQVELRSHIQF
                     YEGIRTSDIHETIIKAAADLITKDTPDYQYLAARLAIFHLRKKAYGQFEPPHLYEHVK
                     KLVALGKYDADLLSDYTPEEWETMNGFIDHWRDMTFSYAAVKQLEGKYLVQNRVSGEI
                     YESAQFLYLLVAACLFSKYPTASRLDYIRRFYDATSTFKISLPTPIMAGVRTPTRQFS
                     SCVLIECDDSLDSINATSAAIVKYVSQRAGIGINAGAIRALGSPIRGGEAFHTGCIPF
                     YKHFQTAVKSCSQGGVRGGAATVYFPIWHLEAESLMVLKNNRGVEENRARHMDYGVQI
                     NKMMYQRLIKGGEISLFSPSDVPGLYEAFFADQAKFEQLYLQYEQDPSIRQRKVKAVE
                     LFSLLMQERASTGRIYIQNVDHCNTHSPFNPRVAPIRQSNLCLEIALPTKPLAHFYDE
                     QGEIALCTLSAFNLGTLSNLDELEELADLVVRALDALLDYQDYPVLAARNSSLNRRAL
                     GVGVINYAYYLAKNQVRYSDGSANNLTHRTFEAIQYYLLKASMNLAKEQGACAYFDQT
                     TYAKGLLPIDTYKKEIDELTQEPLHYDWEQLRQDILQFGLRNSTLTALMPSETSSQIS
                     NATNGIEPPRGYISVKASKDGILKQVVPEYERLKAYYELLWDIPNMDGYLHLVGIMQK
                     FVDQAISANTNYDPNRFAEAKVPMKVLLKDLLTAYKYGLKTLYYQNTRDGAEDVQDDL
                     DEGCAGGACKI"
BASE COUNT         1525 a         1038 c          916 g         1452 t
ORIGIN      
        1 ataccactat tatgcaacgc atttcaggtg caaaacagat agattcactc acggttaaaa
       61 ttaaagatga tattgaaagc caaactgccg aaaaaagcat tgccgaattg ttagtagcta
      121 aacacggcaa aaaagatttc tttattatga atagtgatac gatcaaacaa accattacca
      181 gtaccaccaa tacaatgact ttgctgatct cctctatcgc ccttatttcg ctgattgtcg
      241 gtggtatcgg ggtaatgaat attatgctcg tttccgtcac cgaacgcacc cgagaaattg
      301 gtgtccgaat ggcgattggc gcaaaacaac gcaatatttt gcaacaattt ttaattgagg
      361 cggtggttat ttgcctaata ggtggtgtga ttggcatttt actggcaagc gctattattt
      421 gggcatttaa taccttaggt acgaatttta aaatgatgct ttcaccggaa tcggtgatta
      481 ttgccgtgct ctgctctaca cttatcggtg tggtatttgg ctacattccg gcaagaaacg
      541 ccgcccaatt aaacccaatt actgctctgg cacaagagta aatgcgataa ctatctgcta
      601 cactgtaggg gcaaaaaatt ttttgcccct acatccatta ttatgccaag tttaaggaga
      661 taaaatgaca ataaccaata ctgaactaga acaaattctg aacaccaaac tcaacagctc
      721 cgccattaac gactatgccc caaatggctt gcaagttgaa gggaaaagag agatcaaaaa
      781 aattattacc ggtgtgacag ctacacttcc cctgattgaa aaagcgattg agaaaaacgc
      841 cgatgcaatt ttagtccacc acggttattt ttggaaaagt gaaaacccgt gcattcgagg
      901 aatgaaaggt aaacgcatta aacagctttt agtgaatgac atcaatctct ttggttatca
      961 cctaccactg gatattcact ccgaactagg taacaacgcc cagcttgcta agcatttagg
     1021 agtaaaaaat ctacaaggtt tagaagacag acctaattca attccaatgt acggtgaatt
     1081 agaaaatccg atttccgcca atgaactgaa acaaaaactt gaaaaaacgt tacaacgcac
     1141 cgttatttta tgtgatgaat ttgttcatag cccacaaaaa atgattaaaa caatagggat
     1201 ttgctccggc ggaggacaag gctatattga tttagccttt gaaaaaggct gtgatgcctt
     1261 tattagtggc gaaatttccg aacagaccac tcactctgcc cgtgaacaag gaatctatta
     1321 ttttgcttgc ggtcatcacg ccacggaacg agatggcgta aaagccttag gtgaatggtt
     1381 agccgttgaa tatggtttag atgttgaatt tattgatatt gataaccctg cataaaaaat
     1441 acaagcggtt gaatttcctg aaaattttgc aatttgcaaa aaatcactaa aaatcaaccg
     1501 cttgtatatc atagtctaaa gttcaaaatc gccaaaatca cggctatcta ctttagcatc
     1561 aatctggcca accaaataag aactgacttc cacttcttgt ggtgctacct gtacattatc
     1621 ggaaaccaac cacgcattga tccacggaat ggggttggaa cgtgccttaa atggtaatgg
     1681 tagtccaaca gcctgcatcc gaatattagt gatatattca atatattgca ccaaaatatc
     1741 ccgatttagc cctatcatcg aaccgtcttt aaacaggtat tccgcccacg ctttttcttg
     1801 ttcagctgca cttaaaaaaa gctcgtaggc ctcttggcga cattcacgtg caatttctgc
     1861 catttcagga tcatcattgc cataggccat aatattcaat atatgctgag tgccggtaag
     1921 atgtaatgct tcatcacgtg caataaattt gatgattttg gcatttcctt ccataagctg
     1981 ccgttccgca aaggcaaatg agcaagcaaa agagacataa aaacgaattg cttcaagcgc
     2041 attcacactc atcatacaga gataaagttg tctttttaga ctacgcaaac tgacctcaca
     2101 ggtttttcca tcaacctgat aactgccttc accatacaaa ctatagagtt ggctgtcacg
     2161 aatcaaatca tcataatagg cggaaatatc tttggcacgc ttaataattt cttcgtttgt
     2221 gacaatatca tcaaatacta ttgatggatc attgacaata ttacggatga tatgtgtgta
     2281 agaacgactg tgaattgttt cgctaaatgt ccaagtttca atccaggttt caagctcggg
     2341 gatagacact aatggcaaaa gcgccacatt cgggctacgc ccttgaatgg aatccaacaa
     2401 ggtttggtat tttaaattgc tgataaaaat gtgcttttca tgttcaggca gttgggcata
     2461 gtcaatgcgg tcttgtgaca catccacttc ttccggtcgc cagaaaaatg agagttgttt
     2521 ttcaatcagt ttttcaaaaa tttcgtattt ttgttgatca taacgcgcca cattcacatt
     2581 ttgtccaaaa aacatgggtt cttttagttg atcattttta tgttgtgaaa acgtagtata
     2641 tgccatgtta ttctccttta tttctgttct tattaaattt tacaagcacc accagcacag
     2701 ccttcatcta agtcgtcttg cacatcttct gctccatcac gggtattttg atagtaaagg
     2761 gtttttaacc cgtatttata cgccgttaat agatctttta ataatacttt catcggtact
     2821 ttagcttctg caaagcggtt tgggtcgtaa tttgtattcg ccgaaatcgc ttggtcaaca
     2881 aatttctgca taatcccaac caaatgcaaa tatccgtcca tattaggaat atcccaaagc
     2941 agttcatagt atgcttttaa acgttcatac tccggcacaa cctgctttaa aataccgtct
     3001 tttgatgctt taacactaat atagccacgc ggaggttcaa ttccattggt ggcattcgaa
     3061 atttgcgacg aagtctccga tggcattaag gcagttaaag tggaattgcg caagccaaat
     3121 tgcagaatat cctgacgtag ttgttcccaa tcataatgaa gcggttcttg agttaattca
     3181 tcaatttctt ttttataagt atcgataggg agtaaccctt tggcataagt ggtttgatca
     3241 aaataagcac acgccccttg ttctttggct aaattcatcg atgcttttaa taaataatac
     3301 tgaatcgctt caaaggtacg gtgagttaaa ttattagcac tgccatcgga ataacgcact
     3361 tggtttttag ctaaatagta agcatagtta atcacgccta cacctaatgc gcgacgattt
     3421 aatgagctgt tacgtgcagc taataccgga taatcttgat aatctaatag ggcatctagc
     3481 gcacgaacca ctaaatcagc caattcttct aactcatcta aattgctgag tgtgcctaaa
     3541 ttaaatgccg ataatgtaca taatgcgatt tccccttgtt catcataaaa atgtgccagt
     3601 ggcttagttg gcaaagcaat ttctaagcag aggttcgatt ggcgtatcgg tgcgacacga
     3661 ggattaaatg ggctatgagt attacaatga tcgacattct gaatataaat tcgccctgtt
     3721 gaagcccgct cttgcatcag taatgaaaac aattctacag ctttaacttt acgttgccga
     3781 atgctcggat cttgctcata ttgcaaataa agttgctcaa atttagcttg atcagcaaaa
     3841 aacgcttcat ataaccccgg cacatctgat ggactaaata aactaatctc accgccttta
     3901 attaaacgtt gatacatcat tttattaatc tgtaccccgt aatccatatg gcgagcacgg
     3961 ttttcttcca caccacggtt attttttaat accattaagc tttctgcttc caaatgccaa
     4021 atcggaaaat acacggtagc cgctccaccg cgaacaccac cttgagagca agactttact
     4081 gcagtttgaa aatgtttata aaagggaata cagccggtat gaaatgcttc tccaccacga
     4141 atggggctac ctaatgcccg aattgcgccg gcattaatac cgatcccagc acgctgtgaa
     4201 acatatttaa cgattgcagc cgaggttgca ttgatggaat ctaagctatc atcacactca
     4261 attaacacac aggaactaaa ttgacgagta ggtgtacgca ctcctgccat aatcggtgtt
     4321 ggcaaagaaa ttttgaaagt ggaagtcgca tcataaaaac ggcgaatata atctaagcgg
     4381 cttgctgttg gatacttaga aaataaacag gcagctacga gtaaatacaa aaattgagcc
     4441 gattcataaa tctctccgct aacacgattc tgcactaaat attttccttc caactgcttt
     4501 actgctgcat aggaaaacgt catatcccgc cagtgatcaa taaatccatt cattgtctcc
     4561 cattcttcgg gcgtatagtc gctcagcaag tctgcatcat atttacctag cgcaactaat
     4621 tttttaacgt gctcatataa atgcggtggc tcaaattgtc cataagcttt tttgcgtaag
     4681 tggaaaatcg ccaagcgtgc ggctaaatat tgataatctg gcgtatcctt agtgattaga
     4741 tcagctgcag ccttgataat agtttcatga atatctgaag tgcgaatacc ttcataaaat
     4801 tgaatgtgag aacggagttc tacttgtgaa acagaaacat tctctaaccc ttctgccgcc
     4861 caagtaatca cacgatgaat tttatctaga ttgagttgct cgcgcgtgcc attacgcttt
     4921 gttaccatta a
//
LOCUS       DANLXS010000174         4929 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.175, whole genome shotgun sequence.
ACCESSION   DANLXS010000174 DANLXS010000000
VERSION     DANLXS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.175"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            36..1118
                     /gene="serC"
                     /locus_tag="PXD71_002229"
     CDS             36..1118
                     /gene="serC"
                     /locus_tag="PXD71_002229"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDL6292302.1"
                     /translation="MTEIYNFSAGPAMMPKKVLEKAQQELLNWLDQGTSVMEVSHRGK
                     LFMELAAQSEADLRKLYNVPDNYRILFLQGGARGQFAAIPMNLIGKKGKALYLNSGHW
                     SATAAKEARNFAEIDEINILETEPQLKVSRLDFSDIAEQYDYIHYCTNETISGVEIFD
                     IPNVGNTPLVADMSSNILARGIDINKFGVIYAGAQKNLGPAGITIVIIRDDLIGNARK
                     DTPSIWNYATQRDADSMINTPPTFAWYLCSLVFQHLLAEGGLKATEERNLAKATLLYE
                     YLDSSTFYYNTVAHENRSLMNVTFTTGNDELNAKFVAEATACGLQALKGHKVLGGMRA
                     SIYNAMPIDGVKALIEFMKVFEQRNA"
     gene            complement(1175..2152)
                     /locus_tag="PXD71_002230"
     CDS             complement(1175..2152)
                     /locus_tag="PXD71_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDL6292303.1"
                     /translation="MTLAVAAIIGGLIILVWSADRFVEGAASTARHFGMTPLLIGIVV
                     IGFGTSAPEMLVSASSALSGSPGIALGNAYGSNITNIALILGLTAIIKPLSVNSDVLK
                     KELPILLAVTLLSAYLVYDANVTQLDAIILLAVFFVYMAWTIITAMKSKNDALSLDVD
                     AELTNSAKMGLGKSILWLIIGLVSLVISSQFLVWGAVEVAKFFGVSDLVIGLTIVAVG
                     TSLPELASSIAAARKGEVDLALGNIIGSNLFNTLAVVGIAGAIEPMQVSAEVFSRDVL
                     MMSALTFLLFIFGINLYRRTEGGRINRLEGLLLFAAYVGYNFYLFKTAG"
     gene            2264..4831
                     /gene="glnD"
                     /locus_tag="PXD71_002231"
     CDS             2264..4831
                     /gene="glnD"
                     /locus_tag="PXD71_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDL6292304.1"
                     /translation="MMKNSNELKTLLSAFTEQQRQAFQSENVLSLLAQRSQFYDQLLQ
                     ALWQDFGLAQYEHITLLAVGGYGREEMFPLSDLDILVLTEKPLEEAIQQRLNELFALL
                     WDSKLQLGTSVRTLEECIQIGKAEISVATNMLEGRFLLGNKSLWLRLKEALYQPDFWD
                     IKAFFRAKMAEKNERYARYHNTSYNLEPDLKHSPGGLRDLHLISWIMLRHYGVSSFVD
                     LLNKGLLYPEEYKELIAAQAVLFRMRFALHLQLKRYDNRLRFDRQLQLSEILGYQGEA
                     NQAVELMMRDYFQATKSISQLSQLLLNSFEQAYVNELQKNAQKQPLDQYFYRQEQQLF
                     VVERKIFKTQPTTMLDLFFHLTNDPTLIANADTLRQLRLCLQQQHEPMCFLPEMREKF
                     VQLFAQPKVISRAIVPMHQLGFLSAYLPQWKGIEGLMQFDLFHIYTVDEHTVRVMLNL
                     ENFSEDPSRAFFPLCHQLFATLSEKPLLYLAALFHDIGKGREGEHAQVGALDMREFAE
                     LHGFSQAQINDMVWLVAEHLTMSITAQRRDIHDPQIISEFAKKVQNQTALSSLLCLTV
                     ADICATSESLWNDWKASLFTQLYQFTLQQLAQNLNYQAVAQEHRLQALELMKFVLSAD
                     ERIALNAFWQPCPERYFLHHTPTQLVWHALHYVKTRRLPMVLISNEYARGGTEIFVCC
                     QDQPQLFARIAQLLSQKRISIHDAQIITTENGVVLDSFIVSERDGDDDLTDERCMQIQ
                     QAMMKMLSLTETGIKFTKKPVKHLSFKRKTRLRFLPPSSKQQTEFELFTLDREGLLAQ
                     IGYIFNRLRLNLINAKITTIGERVEDFFVVSNDQGKALSEQEQLELKAILITELDSE"
BASE COUNT         1601 a          963 c         1019 g         1346 t
ORIGIN      
        1 aatagaatca atattattac tatacgaggt gaaaaatgac agagatatat aacttcagtg
       61 ccggcccggc aatgatgccg aaaaaagtac tcgaaaaagc gcaacaagaa ttattaaatt
      121 ggctagacca aggcacatca gtgatggaag tcagccaccg tggaaagcta tttatggagc
      181 tggcggcaca atctgaagca gatttacgca agctgtataa tgtgccggac aattatcgta
      241 ttctatttct tcaaggtggt gcaagggggc aatttgcagc cattccaatg aatttaatcg
      301 gtaaaaaagg caaagcgctt taccttaatt cagggcattg gtcggcaaca gcagcaaaag
      361 aagcacgtaa ctttgcggaa attgatgaaa tcaatatttt agagactgaa ccgcaactta
      421 aagtgagtcg tttagatttc agcgatattg cagagcaata tgattatatc cattattgca
      481 ccaatgaaac catcagtggg gtagaaattt tcgatattcc gaatgtcgga aataccccgt
      541 tagtggcaga tatgtcgtct aatatcttag cccgagggat tgatatcaac aaatttggag
      601 taatttatgc cggagcgcaa aaaaatctag ggcctgctgg cattacgatt gtgattattc
      661 gtgatgattt aattggcaat gcacgcaaag ataccccatc tatttggaat tatgcaactc
      721 agcgagatgc tgattcgatg attaataccc cgccaacttt tgcttggtat ttatgttctt
      781 tagtgttcca acatttactg gcagagggtg gtttgaaagc tactgaagaa cgtaatcttg
      841 ctaaagctac attgctttat gagtatttgg atagttcaac attctattac aatacggtag
      901 cacacgaaaa ccgttcctta atgaatgtaa cctttaccac gggtaatgac gagctaaatg
      961 caaaatttgt ggcagaagcg accgcttgtg gtttacaggc attgaaaggc cataaagtat
     1021 taggtggaat gcgtgcctca atttataacg caatgccgat tgatggggta aaagcactca
     1081 ttgaatttat gaaggtattt gaacaacgta atgcttaatt caagcttaag aatataaatt
     1141 aaagagccac gtttgtggct ctttgaattt ggcactagcc cgcagtttta aacaaataga
     1201 aattatagcc tacataagcg gcaaagagta ataagccctc taaccgatta attctgccac
     1261 cttctgtccg acgatagagg ttgatcccga aaatgaataa taagaaggtg agagccgaca
     1321 tcatcaagac atcgcgagaa aacacttccg ctgaaacttg cattggttct atagcaccgg
     1381 caatccccac cacggctaac gtattaaata agttagaacc gataatgtta cctaaagcta
     1441 aatccacttc tcctttacga gcagctgcaa tagaagaggc taattcaggt agtgaggtgc
     1501 ctaccgccac aatagtaagc ccgattacca aatcactcac accaaaaaat ttagccactt
     1561 caactgctcc ccagactaag aattgggaac taattactag cgatactaaa ccaataatta
     1621 gccaaagaat ggattttcct aatcccatct tagcactatt ggttaattct gcatcaacat
     1681 ctaagcttaa tgcatcattt ttacttttca ttgcggtaat aattgtccac gccatatata
     1741 caaaaaacac agcaagtaaa ataatcgcat caagttgagt gacgtttgca tcataaacca
     1801 aataagcaga aagcagggtg acagccagta aaatcggcaa ctcttttttt aacacatcgg
     1861 aattaactga tagtggttta attattgcgg ttaaacctaa aatcagcgca atattggtaa
     1921 tgttagaacc gtaggcatta ccgagagcga tacccggcga tccgcttaat gcagaagatg
     1981 cagatacgag catctccggt gcagatgtac caaaaccaat gaccacaata ccgattaaca
     2041 atggtgtcat accaaaatgg cgagccgtag aggctgcccc ttccacaaaa cgatccgcac
     2101 tccaaactaa tataattaag ccaccgataa tagcggcaac agcaagtgtc ataaactatc
     2161 cttttagatg aaaaacataa aggcattata agcattttag ccaaaataaa tataggcaaa
     2221 cgaaaataaa tgacctatta ttttgccagt ttaggaagag attatgatga aaaacagcaa
     2281 cgaattaaaa acgcttttat cagcatttac ggagcaacag cggcaagcat ttcaatccga
     2341 aaatgtactc agcttacttg cacaacgtag ccaattttat gaccaactac tgcaagcact
     2401 ttggcaagat tttggactcg ctcaatatga acatattacc ctattagcgg taggggggta
     2461 cggacgtgag gagatgtttc cgctttcgga tctggatatc ttagtgttaa ctgaaaaacc
     2521 gctagaagag gctattcagc agcgtttaaa cgaactattt gccttgcttt gggacagcaa
     2581 actgcaactt ggcaccagtg tgcgtacgct cgaagaatgt attcagattg ggaaagcaga
     2641 aatttcggtt gccactaata tgttggaagg gcgattttta ttgggtaata aatcgctttg
     2701 gctaagatta aaagaagcac tttatcagcc ggatttctgg gatattaaag cgttttttag
     2761 agcgaaaatg gcggaaaaaa atgagcgtta tgcacgttat cataatacta gctataacct
     2821 tgagccagat ctcaaacata gcccgggagg attgcgagat ctgcatttaa tctcttggat
     2881 tatgctacgg cattatggtg tatcttcttt tgtcgatctc ctcaataaag ggctacttta
     2941 cccggaagaa tataaggagc tgatcgcagc acaagcggta ctttttcgga tgcgttttgc
     3001 acttcatttg caactgaaac gttacgataa tcgccttcgg tttgacagac aattacaact
     3061 gagtgaaata ttaggctacc aaggcgaagc aaatcaagcg gtggaattaa tgatgcgaga
     3121 ttattttcaa gccacaaaat ctatttcgca actgagccaa ttattgttaa acagttttga
     3181 acaagcctat gtaaacgagt tgcaaaaaaa tgcacaaaaa caaccgcttg atcagtattt
     3241 ttatcggcaa gaacagcaat tgtttgtggt cgaacgcaaa atatttaaaa cccaacccac
     3301 aacaatgttg gatctctttt ttcatcttac taatgaccct acgttaatag cgaatgccga
     3361 caccttacgt cagttgagac tgtgtttaca gcaacaacac gagcctatgt gctttttgcc
     3421 tgaaatgcga gagaaattcg tacagctttt tgctcaaccg aaagtcataa gccgagcaat
     3481 agttccaatg caccaactgg gttttttaag tgcttatcta ccgcaatgga aggggatcga
     3541 agggctaatg caattcgacc tattccatat ttatacagtc gatgagcata cggtcagagt
     3601 catgttaaat ttagaaaatt ttagcgaaga tccaagtcgg gcgtttttcc cactatgtca
     3661 tcagctattt gcaacgcttt ctgagaaacc gttgctctat cttgcggctc ttttccacga
     3721 tattggcaag ggtagagagg gcgaacacgc tcaagttggt gcgctggata tgcgagaatt
     3781 tgcggaacta cacggttttt ctcaagcaca aatcaatgat atggtttggc tggtggcaga
     3841 gcatttgaca atgtcgatca ccgcacaaag acgagatatc cacgatccgc aaatcatcag
     3901 cgaatttgca aaaaaagtgc aaaatcagac cgctttatct tcactacttt gtttaacggt
     3961 tgcagatatt tgtgcaacca gtgagagcct ctggaatgat tggaaagcct cgctgtttac
     4021 ccaattatat caatttacgc tacagcaact ggctcaaaat ttgaattatc aagcagtggc
     4081 acaagagcat cgcttgcaag cattagaact gatgaagttc gtgctttcag ctgatgagcg
     4141 aatagcatta aacgcatttt ggcaaccttg cccggagcgt tattttcttc accatacacc
     4201 gactcagttg gtatggcacg ctctgcatta tgtaaaaact cgccggttgc cgatggtttt
     4261 aatcagtaat gaatatgccc gtggtggtac agaaattttt gtatgttgcc aagatcaacc
     4321 tcaactgttt gctcgaatcg cccaattact cagccaaaaa cgcattagca ttcacgatgc
     4381 acaaattatt actactgaaa atggtgtagt gttagacagc tttattgtaa gtgagcgtga
     4441 tggtgatgat gatttaaccg atgagagatg tatgcaaatt cagcaggcga tgatgaaaat
     4501 gttaagctta actgaaacag gcataaaatt taccaaaaaa ccggtgaaac atttgtcatt
     4561 taagcgtaaa actcggctgc gttttcttcc tccgtcatca aagcaacaaa ccgaatttga
     4621 actttttacc ttggatagag aagggttgtt ggcacaaata ggctatattt ttaaccggct
     4681 ccgattaaat ttaattaatg cgaaaattac gactattggt gaaagggtag aggatttctt
     4741 tgtggtcagt aatgatcaag gaaaagcttt atcggaacaa gaacagttag aactaaaagc
     4801 cattctaatt accgaactag atagtgaatg aaaacaaccg cttggagtga agactgcaag
     4861 cggtaaattt ttcaacgttt taggtaaatt aaatcacgag ttaagagagg ttgagaattc
     4921 aagaaatta
//
LOCUS       DANLXS010000175         4901 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.176, whole genome shotgun sequence.
ACCESSION   DANLXS010000175 DANLXS010000000
VERSION     DANLXS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.176"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            139..579
                     /locus_tag="PXD71_002232"
     CDS             139..579
                     /locus_tag="PXD71_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL6292305.1"
                     /translation="MTACKNGLGFLVLRLFLAYEFFEAGLSKLNGENWFASIQESFPF
                     PFNVLPADLSWAIASGAEILLPILLTLGLFTRFSALGLMILTAVAWYAVHAGNGYNVC
                     NNGYKMALIYLVMLVPLLLQGAGSLSLDFLLQKKSPTKRWLKFL"
     gene            605..892
                     /locus_tag="PXD71_002233"
     CDS             605..892
                     /locus_tag="PXD71_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292306.1"
                     /translation="MKKATLMALAGALTMATATVNAETKSVKADACMEVKDGKCVKTK
                     ATEGKCGEGKCGATSSEPKATEGKCGEGKCGATAPKAAEGKCGEGKCGASK"
     gene            993..1916
                     /locus_tag="PXD71_002234"
     CDS             993..1916
                     /locus_tag="PXD71_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HDL6292307.1"
                     /translation="MLKFTGAGLGYRRDLADGFLSLTDNNIINFMEVAPENWLKMGGQ
                     ARYNFDKVAEKIPVIIHGLSLSLGGQAPLDMTLLKGIKNMQQQYNSFFFSDHLSYCEC
                     EGHLYDLLPMPFTDEAVHHVANRIKQVQDYLGMQISLENTSYYLHSPTSTMNEVEFLN
                     AIAQEADCGIHLDVNNIYVNGVNHGLLDPFVFIDQVDKSRVNYIHIAGHDEDHGAAED
                     VKMTEGESFNMVKGELRHLPTLLVDTHGEAVQKTVWDLLEYTYEHLPHIPPTLLERDF
                     NFPPFQELISEVQHIMSLQKKYAKTRLKNAA"
     gene            1906..2646
                     /locus_tag="PXD71_002235"
     CDS             1906..2646
                     /locus_tag="PXD71_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HDL6292308.1"
                     /translation="MPLKLVEEHKPLLKEIQAEFANAIRHNDVKLMSGNISPQRLGVY
                     SRLVRNNTLGFIDRCYVQLPQHLSPEEWVAVKEKFIREGKAHSPFFQDIAGEFLNFCR
                     ENGCMPPHLLELMDFENAQLLAEVSMATVPSEFEWDNDTIMQFSGAAELRQYAVNFIR
                     SEFKQFDFEPTNVLIWRDTEFGIYYNRLEELDFFLLSSLQEGANSLNGLLAELSEFTE
                     QNLELKALLRQLWEKWVEAEVIYPVYPE"
     gene            2865..3446
                     /locus_tag="PXD71_002236"
                     /pseudo
     CDS             2865..3446
                     /locus_tag="PXD71_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            3447..3617
                     /locus_tag="PXD71_002237"
     CDS             3447..3617
                     /locus_tag="PXD71_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL6292309.1"
                     /translation="MLKCKQITELVSLSQEQKLTLKQRVNVQVHLMLCSRCRAFSQNC
                     KTVDKLMKEFNS"
     gene            complement(3658..4731)
                     /locus_tag="PXD71_002238"
     CDS             complement(3658..4731)
                     /locus_tag="PXD71_002238"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HDL6292310.1"
                     /translation="MAQQITLFEDVPLPLTLGGELSPITVAYQTYGRLNAEKSNAVLL
                     CHALTGDAKPYYDSPTEKGWWQDFIGKGLAFDTDKYFFICSNVLGGCKGTTGPASINP
                     KTNKPYGSLFPIITVQDIVNVQKALLDYLEIPQLHAVVGGSFGGMQATQWGISYPDKV
                     RNIINLCSSLTLSAEAIGFNHVMRQAIINDPHFNGGNYYDGIAPDNGLKIARMLGMLT
                     YRTDIQLAKAFGRETKQQGQIWGDHFQVESYLSYQGTKFLGRFDANTYLRLLRALDLY
                     DPALGYENEETALKRIKANYTFVAVTSDQLFKQVDVYKSKQKLEQAGVNLEFHEFHSD
                     FGHDAFLVDYDFFEPMIKAGLAK"
BASE COUNT         1480 a          953 c         1042 g         1426 t
ORIGIN      
        1 ctataaaaat aggttataat ttttcacaca ttttttgtaa taaacgtata cttattctgt
       61 ctaataatca catttttagt taggagcatt tttatgtcat cattttcgtc atcattttca
      121 actcagtcag cttgctcaat gacagcttgt aaaaacggct tgggctttct tgtattacgt
      181 ctatttcttg cttatgaatt ttttgaagca gggttatcaa aactaaacgg tgaaaattgg
      241 tttgcctcaa ttcaagagag cttcccattc ccctttaacg tactaccagc cgatcttagc
      301 tgggctattg ctagcggagc ggaaatctta ctgcctattt tactcacttt gggtctgttc
      361 acccgcttca gtgcgttagg attaatgatc ttaacagcgg tagcgtggta tgctgttcac
      421 gctggcaatg gctacaatgt ctgcaacaat ggctataaaa tggcactcat ttatttagtg
      481 atgttagtac cacttttatt acaaggtgct ggctcgctct ctctcgattt tctgttacag
      541 aaaaaatccc caaccaaacg ctggttgaaa ttcctttaat cccttaattt atggagaatt
      601 tttaatgaaa aaagcaactt taatggcatt agctggtgca ttaactatgg caaccgcgac
      661 tgtaaacgct gaaactaaat cagtgaaagc agatgcttgt atggaagtaa aagatggcaa
      721 atgtgtaaaa accaaagcta cagaaggtaa atgtggcgaa ggcaaatgtg gagcaacctc
      781 ttctgagcca aaagcaaccg aaggtaaatg cggcgaaggg aaatgtggag caacggcacc
      841 aaaagcggca gaaggtaaat gcggtgaagg caagtgtgga gcttctaagt aagtagcgaa
      901 ggtgcgtttt ctaaacgcac ctaacctctt ataaacacaa tctacaagcg gttaaattcc
      961 gctgattttt tgcaaattaa aggaacaaga cgatgttaaa atttactggt gccggtctag
     1021 gctatcgccg tgacctagcg gacggatttt tatctctcac agataacaat ataattaatt
     1081 ttatggaagt agccccagaa aactggctga aaatgggcgg tcaggctcgg tataacttcg
     1141 ataaagtcgc tgaaaaaatc cctgttatta ttcacggttt atccctctct ctcggcgggc
     1201 aagcgccttt agatatgacg ctactaaaag ggatcaagaa tatgcagcag cagtataact
     1261 cctttttctt ctctgatcat ttaagctatt gcgaatgtga agggcattta tatgacctgc
     1321 tgccaatgcc gtttaccgat gaagccgtac atcacgtggc aaatcgcatt aaacaagtgc
     1381 aagattattt aggtatgcaa atttcccttg aaaatacgtc ctactatctg cattcaccaa
     1441 ccagtacgat gaatgaggta gagtttctca atgcaattgc ccaagaagcg gattgtggca
     1501 ttcaccttga tgtgaataat atttatgtta atggtgtcaa tcacggcttg cttgatccat
     1561 ttgtatttat tgatcaagtg gataaatctc gtgtcaatta tattcatatt gcaggccacg
     1621 atgaagatca cggtgcggcg gaagacgtta aaatgaccga gggtgaatca tttaatatgg
     1681 taaaaggcga actacgtcat ctgccaactt tactggtcga tacacacgga gaagctgtac
     1741 aaaaaactgt gtgggattta ctagagtaca cctatgaaca cctaccgcat attccaccaa
     1801 cactgttaga acgggatttc aacttcccac cgtttcaaga actgattagt gaagtacagc
     1861 acattatgag cttacagaaa aaatatgcaa aaacgaggct aaaaaatgcc gcttaaactc
     1921 gttgaagaac acaaaccgtt actcaaagag attcaagccg agtttgcgaa tgcaattcgc
     1981 cataacgatg taaagctgat gagtggtaat atctctccac agcggcttgg cgtctattct
     2041 cgcttggttc gcaataatac tctcggtttt atcgaccgtt gttatgtaca gctccctcaa
     2101 catctaagtc ctgaagaatg ggttgccgtt aaagaaaaat ttatacgaga aggtaaggct
     2161 cattcgccct ttttccaaga tattgctggc gaatttctca atttttgccg agaaaatggc
     2221 tgtatgccac cacacttgct tgagttgatg gactttgaaa atgctcaatt actggctgag
     2281 gtatcaatgg caaccgtacc atcagaattt gaatgggata acgatactat aatgcagttt
     2341 tctggtgcag ctgagctgcg tcaatatgcg gtaaacttta tccgtagcga gttcaaacag
     2401 tttgactttg agccgaccaa cgtactgatt tggcgagata cggaatttgg catctattac
     2461 aaccgcttgg aagagctgga tttcttcctg cttagctcgt tgcaagaggg ggcaaattcc
     2521 ctgaatggct tactagcaga actcagcgag tttaccgagc aaaatctgga gctaaaagcc
     2581 ttgttacgcc aactttggga aaaatgggtt gaagcagagg tgatttatcc tgtctatcct
     2641 gaatgaggat aaggtatgtg atatgtactg acatacatag taggggcggg tcctgtgccc
     2701 gcccgactaa tattaagata acgggcgggc acaggacccg cccctacgat tagtatgtta
     2761 ggtttaaaaa tacttcatac caatatcccc taagttgggt aaaaccttat gaaataggat
     2821 acaagcggtt agtttctctc aattttttac caaaggatca cgcaatgagc caaccggctt
     2881 tacggcaaat ttccccctcg cagctagaag atattcgtct gcaaatggtg aagtttgcct
     2941 ttttacagtt aaaaaataac gaacttgccg aagatgtggt gcaagaggcg tttgtcagcg
     3001 cttataaata tgccgacagc ttcaaaggtg aaagtgcgtt aaaaacgtag atttttgcaa
     3061 ttttaaaaaa caagatcgta gatttagtga aagccaaagg caaattggtg gcagtatctg
     3121 agctgatgga agaagacgat cacgatcttg ccgataaatt gtttaacgaa aatggcggtt
     3181 gggataaaga tgcttatcaa tctaatgagt ggaagaacac cgaaagccat acctattccg
     3241 ctcaattctg gcaagtattt gaattttgcc taaataatct gccggcaaat caagcaaggg
     3301 tgtttatgat gcgttcccat ttggaaatgg aaaccgaaga aatttgcgca gaatgtgaga
     3361 tttcaccagc aaatgtgcat accttactgt atcgggcaag attgcagtta caggtctgtt
     3421 taagtcaaaa atggtttgga ggttaaatgt taaaatgtaa gcaaattacc gaattagttt
     3481 ctctgtccca agaacaaaaa ctgacattaa aacaacgtgt taatgtgcaa gttcatttaa
     3541 tgctttgttc tcgttgcaga gcctttagcc aaaactgcaa aaccgtagat aagctaatga
     3601 aagaatttaa ctcttagcgt aaaagcaagc ggtaaaattt ttaataaaaa atgcaattta
     3661 ttttgctaat ccggctttaa tcatcggttc aaaaaagtca taatcaacca aaaaggcatc
     3721 gtgtccgaaa tcggagtgaa actcgtggaa ctccaaattt acgcctgcct gttctaattt
     3781 ttgtttgctc ttatacacgt caacttgctt gaatagttgg tcgcttgtta ccgctacaaa
     3841 agtgtagttg gctttaatac gttttagggc ggtttcttca ttttcatagc caagagccgg
     3901 gtcgtataaa tctaatgctc ggagtaaacg gaggtaagtg ttggcatcga aacgacctaa
     3961 aaatttcgtg ccttgatagc ttaaatagga ttccacttgg aaatggtcgc cccaaatttg
     4021 tccttgctgt ttggtttctc gtccaaaggc tttagcaagt tgaatatcag tacggtaagt
     4081 cagcataccg agcatacggg cgatttttaa gccgttatca ggtgcaatgc catcataata
     4141 gttcccaccg ttaaagtgtg gatcgttaat aatagcttgg cgcataacgt ggttaaaacc
     4201 gatagcctcg gcacttaggg taagtgacga acaaagattt ataatgttgc gaactttatc
     4261 agggtaacta ataccccact gtgttgcttg cattccgccg aaagatcccc ctaccactgc
     4321 gtgaagttga gggatttcta aatagtccaa taatgctttt tgtacattaa caatatcttg
     4381 cactgtaata attggaaaca aactaccgta aggtttattg gttttagggt taatcgaagc
     4441 tgggccggtt gtgcctttac aaccgcctaa tacattactg caaataaaga aatatttatc
     4501 ggtatcaaac gcaagccctt tgccgatgaa atcttgccac cagcctttct cggttggtga
     4561 gtcatagtaa ggcttagcat caccggtaag ggcgtggcaa agcaagaccg cattgctttt
     4621 ctctgcattt aatctgccgt aagtctggta agccactgtg ataggggaca gttcgcctcc
     4681 taatgtgagt ggtaaaggta cgtcctcaaa aagggtaatc tgctgagcca taaaaatcct
     4741 gtttgcaaaa aattctgctt ttttgaccgc ttgttgtaat gaccgttaaa ttagcggtgt
     4801 tcatttcaat tgtcaagaat ttttagacgt ctaaacgtct agatttttaa acggctaaaa
     4861 tcaaaatttt attgctgaga agatatttat tacttaacaa a
//
LOCUS       DANLXS010000176         4901 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.177, whole genome shotgun sequence.
ACCESSION   DANLXS010000176 DANLXS010000000
VERSION     DANLXS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.177"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(29..739)
                     /locus_tag="PXD71_002239"
     CDS             complement(29..739)
                     /locus_tag="PXD71_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HDL6292311.1"
                     /translation="MFMVIQLALYNLQTYEVGRNATTIFMTTDKVYTGGSTWTYLFAD
                     ADNKITSVREMNGFGDVEKEFKCGEYKEAVTIASKEDLAKRKTQPRLWSSIYMSPEQN
                     MMWVTDNTSKNLENAKQDAFKQCEKNGGRNCQMILSFSNMCLAFAQGYKDGQGFEDFG
                     YGIVSSLAEENALENCNQKGGSSCLLSAPAVCAIPCDILSNNKCMYDQPQIIIPGKNN
                     NQPINPPANGNGEHWLSN"
     gene            complement(708..854)
                     /locus_tag="PXD71_002240"
     CDS             complement(708..854)
                     /locus_tag="PXD71_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292312.1"
                     /translation="MIKPLDSVYWRGKDVSEFFARKRLPDTAVQCVKDNELSYVYGYS
                     TSFV"
     gene            complement(972..2189)
                     /gene="fabB"
                     /locus_tag="PXD71_002241"
     CDS             complement(972..2189)
                     /gene="fabB"
                     /locus_tag="PXD71_002241"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDL6292313.1"
                     /translation="MKRVVITGLGVISSIGNNKEEVLESLKAGKSGIEFVPEFAEVGM
                     RSQVAGTIKLNPAELIDRKIYRFMGDAAAYAYLSMKEAIEDSGLTEEQVSNDRTGLVI
                     GAGTGSAHSQLVACDAVRGPRGVKAVGPYAVTKTMASSVSACLATPYKIRGVNYSISS
                     ACATSAHCIGHAMELIQLGKQDVVFAGGAEELSWECATEFDAMGAVSTKYNETPTKAS
                     RAYDANRDGFVIAGGGAVVVVEELEHALARGAKIYAEIVGYGATSDGYDMVAPSGEGA
                     ERCMRQAMATVNGEIEYINVHGTSTPVGDVKELGAIRNVFGDKTPAISSTKSMTGHSL
                     GAAGAHEAIYSLLMLDNNFIAPSINIETLDEQAQGMNIVTERQDKALTTVMSNSFGFG
                     GTNACLVFQRYSK"
     gene            2450..>4901
                     /locus_tag="PXD71_002242"
     CDS             2450..>4901
                     /locus_tag="PXD71_002242"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL6292314.1"
                     /translation="MKTLTLTANKITAVEGAAIGANQGSFAQKLNSTKARIDQLNAYR
                     IERAINANNHHFMHVFSLIPLLIHLNHPVLPAYVENAPQGIWHFQLSDYQKQFLVSYR
                     FDEAISADHLGPHPSFDALYSMGSTGTITQTSCSDLDLWLCYPNHFTIEQYKLMELKI
                     NKLQEWAKTFQIDINIYLMNPEQFKSQTYNSSVTDEHSGSAQHFFLLDEFYRSAILLA
                     GKRLLWLHLHKNEYQNVHNNPEIDLTEWIDFGDFSSLSTSEFFGASLWQLYKGINNPY
                     KSAIKILLLECYAHTYPKTKLISKEFKKKLLSDNALEYHFDPYLAMLELVTEHLRSRK
                     EWVKLDRLRVCFYAKAVEGEINNNWRSRDLDELVNQWSWSEKQIQLLQNRQHWKIKQV
                     MNHENMLIDHLLQSYRNLIHFARKFHINPSIMTNDTDILMRKLYSVFEALSGKISLIN
                     HKISSNLAEKAITFIEAKVGSALQPGWYLVNQAPKSRYDSENRYVHYNKSPIKLIAWG
                     YFNGIINAATNLHTVSPNLDIEKLRQFITDLRLSFPIQAPEVTNDDMHHPNEIRNLVI
                     ALNLVQDPTEHIDELPKKVYPSDLFNFGKAQKTLVGSVSIIYRNVWNEIRTQHFDGED
                     AILKALKLVSNRIYQGNSSPQSVNVFCYSKYLRSELRDFVADLVHKCITIQTGCLLQE
                     NEFSTLKVAGKTWQFLFHKKTFSIKQIDEQQAVENLNSFAKTKQNAKSENEKKKEFPQ
                     QISEFASEGFLQFFFEDNVDNSFNVYVVDERNNVESYYNCFGNKEEKIRKINKLQNEK
                     QLKTETPASFGSFNHPQFY"
BASE COUNT         1593 a          991 c          802 g         1515 t
ORIGIN      
        1 gcgtttccgc actttttact aaaatgattt aattactcaa ccaatgctca ccgttgccat
       61 tagctggtgg attaatcggt tggttattat tcttacctgg aataataatt tgaggctgat
      121 cgtacataca tttgttgttc gacaatatat cacagggaat agcacatacg gcaggggcag
      181 aaagaagaca acttgaacca cctttttgat tacaattctc taatgcattt tcttcagcaa
      241 gactactaac gataccatac ccaaaatcct cgaagccttg accatcttta taaccttgtg
      301 caaaagcaag gcacatatta ctaaacgata agatcatttg gcaattacga ccaccgttct
      361 tttcacactg tttaaacgca tcttgcttcg cattttctaa atttttagac gtattatcag
      421 tcacccacat catattttgt tcaggtgaca tataaattga tgaccataaa cgaggctgtg
      481 ttttacgctt tgctaagtcc tctttactgg caatggttac cgcttcttta tattcaccac
      541 atttaaactc tttttcgaca tcaccaaaac cattcatttc acgaacagaa gtgattttat
      601 tatcagcgtc agcaaaaaga tatgtccaag tcgatccacc tgtataaacc ttatctgttg
      661 tcataaatat agtggttgca ttacgaccaa cttcataagt ttgcaaatta tacaaagcta
      721 gttgaataac cataaacata gcttaattca ttatctttta cgcattgaac cgctgtatca
      781 ggtaaacgtt tacgtgcaaa aaattcagat acatctttac cacgccaata aacactatct
      841 agtggcttaa tcattgtaga acaagcggtc aaaaataatg acaaaataac aaatagcaaa
      901 gaacttattt tgaaatttat aataattacc tcaaaagaaa aaagagcaag ctttcacttg
      961 ctctttttat gttacttaga atagcgttgg aacactaaac acgcattagt accaccaaag
     1021 ccgaagctgt ttgacatcac ggttgttaat gctttgtctt ggcgttcagt cacaatattc
     1081 ataccttgtg cttgttcatc aagggtttca atattaatgc ttggtgcaat aaaattatta
     1141 tctaacatta ataatgaata aattgcctcg tgcgcacctg ctgcacctaa tgagtgtccg
     1201 gtcattgatt tggttgatga aattgctggc gttttatcac caaagacatt acggatcgcc
     1261 cctaactctt ttacatcacc cactggagta gaagtgccgt gtacgttaat atactcgatt
     1321 tcaccattta cagttgccat cgcctgtctc atacaacgct cagcaccttc accacttggc
     1381 gcgaccatat cgtaaccgtc tgaggttgcc ccatagccca caatttcagc atagattttg
     1441 gcaccacgag caagagcgtg ttctaactct tccaccacaa ccactgcacc accaccggca
     1501 atcacaaaac cgtcacgatt agcatcataa gcacggctag cttttgttgg tgtttcatta
     1561 tatttagttg aaacagcacc cattgcatca aactcggttg cacattccca agaaagttct
     1621 tctgcaccac ctgcaaacac cacatcttgt ttacctaatt ggattaactc catcgcgtgt
     1681 ccaatacagt gtgctgaagt cgcacaagct gagctgattg aatagtttac accacgaatt
     1741 ttataaggtg ttgccaagca tgctgacaca cttgatgcca ttgttttggt tactgcgtaa
     1801 ggcccaacgg ctttcacacc acgtgggcca cgtactgcat cacaagctac aagttgactg
     1861 tgcgccgagc ctgtaccagc tccgatcacc aaaccggtac gatcgtttga aacttgttct
     1921 tcggttaagc ctgagtcttc gattgcttct ttcattgaaa gataagcgta agccgcagca
     1981 tctcccataa aacgatagat tttacggtca attaactcag ccggatttaa tttaatcgta
     2041 cctgccactt ggctacgcat acccacttca gcaaactcag gcacaaattc aatgcctgat
     2101 ttacctgctt ttagtgattc caacacttct tctttgttgt taccgatact ggaaattaca
     2161 cctaatcctg taataacgac tcttttcatt ttgtttcctt catcattttg atctgaaaaa
     2221 tacttgattc agcttacact tgttcactca aataagctac taaaacttga ttattttaca
     2281 caatttcatt actggtctga actctttcta tcaagatagc ggttgaattt cagacatttt
     2341 ttgcaaaata gggaatgaaa tttaatttcc cgatttttta ccctattaca taataatcac
     2401 ttttccgcta aaatgctaaa cagttcacac tttagtaagg aaagctgacg tgaaaacatt
     2461 aaccctgaca gccaacaaaa taacagccgt agaaggcgct gccattgggg cgaaccaggg
     2521 gagcttcgcc caaaaattaa actctactaa agcacgaatt gatcaactta atgcataccg
     2581 aatagaacga gctatcaatg ctaataatca tcattttatg catgttttct ctctcattcc
     2641 attgcttatt catcttaatc accctgtgtt accggcttat gttgaaaatg caccacaagg
     2701 gatttggcat tttcaactct ctgattatca aaaacaattt ctggtttctt accgttttga
     2761 tgaagcaatc tctgccgatc atttgggccc ccatcccagc tttgatgctc tttattcgat
     2821 gggtagcacc ggtactatta cccaaacaag ctgttctgat ttagatttat ggctatgcta
     2881 tccgaaccat tttacaattg aacaatataa gttaatggag ctcaaaatta ataaattgca
     2941 agaatgggca aagacattcc aaatcgacat caatatttat ttaatgaacc cagaacaatt
     3001 taagagccaa acttataaca gtagcgtaac cgatgaacac agtggttcag cacagcattt
     3061 ttttctatta gatgaatttt atcgttcagc tattttactt gccggtaagc gtttactctg
     3121 gttgcattta cataaaaatg aataccaaaa tgttcacaat aaccctgaaa ttgacttaac
     3181 ggaatggatt gatttcggcg atttttccag cctttcaacc agtgaatttt ttggtgcaag
     3241 cttatggcag ctctacaaag gaattaataa cccttataaa tcggcaatta aaattttatt
     3301 gctcgaatgc tacgctcaca cttatcctaa aacaaagctc atctccaaag aatttaagaa
     3361 aaaactacta agtgacaatg ctttggaata tcactttgat ccctacttag ccatgcttga
     3421 attggtaaca gaacacttaa gatcacgcaa agagtgggtg aaattagacc gcttgcgagt
     3481 ctgtttttat gcaaaagcgg ttgagggaga aataaataac aactggagaa gtcgtgattt
     3541 agacgagctg gttaaccaat ggtcttggtc agaaaaacaa attcaattac ttcaaaaccg
     3601 tcaacattgg aaaattaagc aagtgatgaa tcatgagaat atgctgattg atcatttact
     3661 acaaagttat cgaaatttaa ttcattttgc tcgaaaattt catattaatc cgagcattat
     3721 gacaaatgat actgatattt taatgcgaaa actttattct gtatttgaag cactttcagg
     3781 aaaaatatct ttaattaatc ataaaatatc gtccaattta gcagaaaaag caattacatt
     3841 tattgaagct aaagttggat ctgcgttaca acccggttgg tatttagtta atcaggcacc
     3901 taaaagtcgt tatgactctg aaaatcgtta tgtccattat aataaaagcc cgattaagct
     3961 tattgcttgg gggtatttta acggcattat taacgcagca actaatttac atactgttag
     4021 ccctaattta gatatcgaga aattgcgaca atttatcacc gacttgcgct tatctttccc
     4081 aattcaagcg ccggaagtga ccaatgatga catgcatcac cctaatgaaa ttcgcaattt
     4141 ggttatagcg cttaatttag tacaagatcc aaccgaacat atcgatgaac taccaaaaaa
     4201 agtttatcca agcgatctgt ttaattttgg taaggcacaa aaaacgttag ttggtagtgt
     4261 gagcattatt tatcgaaatg tatggaatga aattcgaact caacattttg acggtgaaga
     4321 tgctatttta aaagcactaa aattggtatc caatcgaatt tatcaaggaa attcttcacc
     4381 tcaatcagtt aatgtttttt gctatagtaa atatctacgt agtgagttac gtgattttgt
     4441 ggcagattta gttcataaat gtattacaat tcagactggt tgtttactgc aagagaatga
     4501 atttagcact ttaaaagtag caggaaaaac ctggcagttt ctttttcata aaaaaacatt
     4561 tagcattaaa caaattgatg aacaacaagc ggttgaaaat ttaaattctt ttgcaaaaac
     4621 caagcaaaat gctaaaagcg aaaatgaaaa gaaaaaagaa tttccgcagc aaattagtga
     4681 atttgccagt gaaggctttt tacagttttt ctttgaagac aatgtcgata atagttttaa
     4741 tgtatatgtt gtagatgaac gtaataatgt cgaaagttac tataactgtt tcggcaataa
     4801 agaagagaaa atcaggaaaa tcaataaatt acaaaatgaa aaacaattaa aaacagaaac
     4861 accagcatca tttggaagct ttaatcatcc acaattttat c
//
LOCUS       DANLXS010000177         4889 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.178, whole genome shotgun sequence.
ACCESSION   DANLXS010000177 DANLXS010000000
VERSION     DANLXS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.178"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(361..2031)
                     /gene="glnS"
                     /locus_tag="PXD71_002243"
     CDS             complement(361..2031)
                     /gene="glnS"
                     /locus_tag="PXD71_002243"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDL6292315.1"
                     /translation="MSEEILTAETGTRANFITHIIDEDLASGKHNNVYTRFPPEPNGY
                     LHIGHAKSICLNFGIAQDYNGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEP
                     RYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRSVEENL
                     ALFEKMKNGEFAEGKACLRAKIDMASPFIVMRDPVLYRVKFAHHHQTGDKWCIYPMYD
                     FTHCISDAIERITHSLCTLEFQDNRRLYDWVLDNISIERPLPHQYEFSRLNLEGTLTS
                     KRKLLKLVTDGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSAL
                     EACIREDLNENAPRAMAVINPVRVVIENFGEKEILKAPNHPNRPELGERDLPFTREIY
                     IDEADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGRISTIFCTYDPDTLGK
                     NPADGRKVKGVIHWVSAEDNKPAEFRIYDRLFTVPNPGAEEDINAVLNPESLVVKHGF
                     VESSLAHAKPEQDYQFEREGYYCLDSKDGSADNLVFNLTVSLKESVAF"
     gene            complement(2192..2635)
                     /locus_tag="PXD71_002244"
     CDS             complement(2192..2635)
                     /locus_tag="PXD71_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL6292316.1"
                     /translation="MNLQQIQDRLALKDLVDTFSNLADEKKVAEQMPLFTQDAVVNTY
                     IGGKLVFEMHGVAEIEQVFTTFLTPFHSVYHLNGQHTVPFIDENNATAINYCAVKLVE
                     TKEGKEILHDHSVRYADTYVKQEGKWRIAKRIANFMISESREIRQ"
     gene            complement(2686..3261)
                     /locus_tag="PXD71_002245"
     CDS             complement(2686..3261)
                     /locus_tag="PXD71_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL6292317.1"
                     /translation="MKQILILGASGSLANVVIPELLKDNNVQLTLFARRPQSVAKFET
                     ERVKIVQGDVLDFHALTQAMQGVDAVYAGLAGDLKAMAGNVVNAMNEAQVKRLVWISS
                     MGIYGETGEDHGAILEPYRQSAAVIEASELDYTVVRPGWFTNGSEVQYQLTHKGEPFK
                     GHSVSRKSIADFVARILRENSEIRESVGIAA"
     gene            3368..4258
                     /locus_tag="PXD71_002246"
     CDS             3368..4258
                     /locus_tag="PXD71_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6292318.1"
                     /translation="MKENLNDLRAFLLVAQTSSFTKAAAQMGVSQSALSHSIRGIEER
                     LQIKLFHRTTRSISTTEAGERLYQRLLPLFDEIDQEVNGLSEFRNALKGGLRINGNEH
                     VFRDVLADKFAEFVKRYPEVALELVAEDRFVDIVAERFDAGIRLGADVAKDMIALRIS
                     PDVKMTAAASPNYLAQHGTPKTPFDLTEHHCLKHRLATLGGILAWEFRDPITKKIVKF
                     QPQGNLTANNGFLLQHYAKQGLGILWSPQDSIQTELDNGSLVPILTDWAMQYEGYHLY
                     YPNRRQNSPLLKALIDVLRG"
     gene            complement(4325..4846)
                     /gene="efp"
                     /locus_tag="PXD71_002247"
     CDS             complement(4325..4846)
                     /gene="efp"
                     /locus_tag="PXD71_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL6292319.1"
                     /translation="MQDGEPCVIVENEFVKPGKGQAFTRTKIRKLISGKVLEINFKSG
                     TSVEAADVVDSNYNYSYSDGDFWYFMHPETFEQISVDEKALGDNVKWLVDNAECIVTL
                     WNGSAIAVTPPNFVELEIVETDPGLKGDTAGTGGKPATLSTGAVVNVPLFVQIGEVIR
                     VDTRSGEYVSRVK"
BASE COUNT         1338 a         1073 c         1032 g         1446 t
ORIGIN      
        1 tcgagaagac aatgctatca taaaagagca tcacttttgg tttccaaaga ttatagaaag
       61 ctcaaataat acactacctc ataatttaaa atgaaacgtt tcaattggaa aaagtggatt
      121 ttgtgactga gatcacaaat ttaaacattg gtctgtattt ttgagagatt ttgtttgaaa
      181 ctgccggcaa attcgcttaa ccttaaggta acaagaataa gtgttgtacc tagcaaccta
      241 acacataaat ttgctttccg agtggcccca agaattgggg ctttttttat gcttgatgag
      301 tcgttttgag agtaaaaaaa taccagcata tttgctggta tttgaatgaa gagagttaga
      361 ttagaacgct acgctttctt ttaagctgac agttaagtta aatacaaggt tgtctgccga
      421 accatctttg ctgtctaagc agtagtaacc ttcacgctcg aattggtagt cttgttccgg
      481 tttggcgtga gctaaacttg actcaacaaa accatgctta accactaatg attcaggatt
      541 tagcaccgca ttgatgtctt cttccgcccc cgggtttggt actgtaaata ggcggtcgta
      601 aatgcggaac tctgccggtt tgttatcttc agcagatacc caatgaatca cgccttttac
      661 tttgcgacca tcagccggat ttttgcccaa tgtatccggg tcgtatgtgc aaaaaatagt
      721 tgaaattcga ccgcttgcat ctttttccac acgctcagct ttaatcacat aggcattgcg
      781 taaacgaact tctttgccta agactaaacg tttatattgc ttattggctt cttcacggaa
      841 gtcggcttca tcaatataaa tctcacgggt gaacggtaaa tcacgctcgc caagctctgg
      901 acgatttggg tgattagggg cttttaatat ttccttttca ccgaagtttt caatcaccac
      961 tcgaaccggg ttaatcaccg ccatcgcgcg tggtgcgttt tcatttaagt cttcacgaat
     1021 gcacgcttca agggcagagt attccaccac gttatcttgc ttggttaccc caatacggcg
     1081 acaaaattca cgtaaagacg ctggggtata accacggcgg cgtaagccgg aaatggttgg
     1141 catacgaggg tcattccaac catcaacaat gccgtctgtg actaatttta ataatttacg
     1201 cttagaggtt aaagtacctt ctaaatttaa acgtgaaaat tcgtattggt gcggtaacgg
     1261 tctttcaatc gaaatattgt ccagcaccca atcatataag cggcggttat cttggaactc
     1321 tagcgtacat agcgagtgag tgattcgctc aatcgcatcg gaaatgcagt gggtaaaatc
     1381 gtacatcggg tagatgcacc atttatcacc ggtttgatga tggtgagcaa atttcacacg
     1441 gtaaagcacc ggatctcgca tcacaataaa cggggacgcc atatcaattt ttgctctcaa
     1501 gcaggctttc ccttccgcaa actctccgtt tttcattttt tcgaataacg ctaagttttc
     1561 ttccacgcta cgatcacggt aagggctgtt tttgcccggc tcggttaaag taccacggta
     1621 ttcacgcatt tcatcaggcg ataattcatc aacataggct aatcctttgt tgattaactc
     1681 aatcgcatag ccataaagct ggtcaaaata gtctgaggca taacgaggct caccctccca
     1741 tttaaaacct agccactcca catcttgctt aattgaatct acatattcca cgtcttcttt
     1801 aaccgggttg gtatcatcaa aacgcaagtt acataaaccg ttataatctt gagcgatacc
     1861 aaaatttaaa cagatagatt tagcgtgtcc gatatgcaaa taaccgtttg gttcaggtgg
     1921 aaaacgagtg taaacattgt tgtgctttcc gcttgctaaa tcttcatcaa taatatgggt
     1981 aataaaattg gctcgagtgc cagtttctgc cgttaaaatt tcttcgctca tagttctctc
     2041 taaatgtatc aataaaaata acggattatt ctacacttaa ttgaaacaga cttaaagact
     2101 aaagcaaaat tcctcggttt cctccaaaga attttgctgt tttagtaaga tgcaatggaa
     2161 ttgcaaattt ttagctaaat ttaaccgctt gttattgcct gatttcacgg ctttcgctga
     2221 tcataaaatt cgcaatccgt ttggcaatcc gccatttacc ctcttgttta acataggtat
     2281 cggcataacg cacgctgtga tcgtgcaaga tctctttgcc ctctttggtt tctaccagtt
     2341 tcaccgcaca atagttgatc gctgtggcgt tattttcatc aataaaaggc acggtatgct
     2401 gaccgtttaa atgataaacg ctgtgaaacg gggttaaaaa ggtggtaaac acctgttcga
     2461 tttctgccac gccgtgcatt tcaaacacca attttccgcc gatgtaggtg tttacgacgg
     2521 catcttgcgt gaaaagcggc atctgctcgg caaccttttt ttcatcggca aggttagaga
     2581 atgtatctac taaatctttt agtgctaaac gatcttggat ttgttgtaag ttcattgtgt
     2641 tttccttctg atttttgcaa attttctttg gtttttcacc gcttgttagg ctgcaatacc
     2701 aacactttca cggatttcgc tgttttcacg caaaatgcga gccacaaaat cggcaatgga
     2761 tttgcgggaa acgctgtgtc ctttgaacgg ttcgcctttg tgagtcagct gatattgcac
     2821 ttccgaaccg ttggtaaacc agcccgggcg gacaacggta taatccaact cgctggcttc
     2881 aatcacggct gccgattggc ggtaaggctc taaaattgcc ccgtgatctt cgccggtttc
     2941 gccataaatc cccattgagc taatccaaac caagcgtttt acctgagctt cattcattgc
     3001 gttcaccaca tttcccgcca tcgcttttaa gtcgccggca agtccagcgt aaacggcatc
     3061 aacgccttgc attgcttggg ttaatgcgtg gaaatccaac acatcgcctt gcacaatttt
     3121 gacgcgttca gtttcaaatt tcgctactga ttgcggacga cgggcaaaaa gggttaattg
     3181 cacattattg tcttttagca gttcaggaat cacgacattc gctaggctac cgcttgctcc
     3241 taaaattaag atctgtttca ttgctgttct ccattgaatg aagtcagtat agcggtgtag
     3301 attgcatttg attagtaggg taaaatcgct tgaagtaatg agaaaaattc attaataagg
     3361 tggttaaatg aaagaaaacc tcaacgattt aagagctttt ttgttggtag cacaaacgag
     3421 cagttttacc aaagcagcag cacagatggg cgtgtcgcag tcggcgttgt cgcacagcat
     3481 tcgggggatt gaagaacggt tgcaaatcaa gctgtttcat cgcaccactc gtagcatttc
     3541 aaccaccgag gcaggggaga ggctgtatca acggctgtta ccgctgtttg atgaaatcga
     3601 ccaagaagta aatgggctga gtgagtttcg caatgcgtta aaaggcgggc tgagaattaa
     3661 cggtaatgaa cacgttttcc gtgatgtgct ggcagataaa ttcgctgagt ttgtcaaacg
     3721 ttaccctgaa gtagcattgg agctagtggc ggaagatcgc tttgtagata ttgtcgccga
     3781 gcgttttgat gccggtattc ggcttggggc agatgtggcg aaagatatga ttgcactacg
     3841 catttcgccc gatgtgaaaa tgaccgcagc tgccagcccc aactatttgg cacagcacgg
     3901 cacgccgaaa acgccctttg atttaaccga acatcattgt ctcaaacatc gtcttgcgac
     3961 attgggcggc attctggcgt gggaatttcg ggatcccatc accaagaaaa tcgttaaatt
     4021 tcaaccgcag ggcaatctta ctgccaataa cggcttcttg ctgcaacact atgccaaaca
     4081 aggtttaggc attctttgga gtccgcaaga cagcattcaa accgagctgg ataacggcag
     4141 cctcgtgccg attttaaccg actgggcaat gcagtatgag ggctatcatc tctattaccc
     4201 caaccgtcgc caaaattcgc ctttgctgaa agcgttaatt gatgtgctga gaggataggt
     4261 aaaaatgagc ggtcgttttt agcaaaaaat tgaccaaaaa acgaccactt gtagagaagc
     4321 taaattattt cacacgagaa acatattcgc ctgaacgggt atccacacgg ataacttcac
     4381 cgatttgaac gaataaaggc acgtttacca cagcaccggt gcttaatgtt gccggtttac
     4441 cgcctgtacc tgcagtatcg ccttttaagc ccgggtcggt ttcgacgatt tctaactcaa
     4501 cgaagtttgg tggtgtgact gcaattgctg aaccgttcca taaagtcacg atacattctg
     4561 cattatcaac taaccatttt acgttgtcgc ctaacgcttt ttcatctacc gagatttgtt
     4621 caaaggtttc agggtgcata aagtaccaga aatcaccatc gctgtaagag tagttgtagt
     4681 tagaatctac tacatctgct gcttcaaccg aagtgcctga tttgaagttg atctctaata
     4741 ctttgccaga gattaattta cggattttag tacgggtaaa tgcttgacct ttacccggtt
     4801 ttacgaattc gttttcaaca attacacaag gttcgccatc ttgaataaat tttaaacctt
     4861 ttttaaagtc gttagtgctg tagctagcc
//
LOCUS       DANLXS010000178         4839 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.179, whole genome shotgun sequence.
ACCESSION   DANLXS010000178 DANLXS010000000
VERSION     DANLXS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.179"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            9..494
                     /locus_tag="PXD71_002248"
     CDS             9..494
                     /locus_tag="PXD71_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HDL6292320.1"
                     /translation="MKIKTLSLIMLLSVTVNAAPVELHQFDSPKQESDYRALIQELRC
                     PQCQNNNIADSNATIATDMRAKTLELLKQGKSKDEVVDYMIERYGNFVTYDPPLTPAT
                     IMLWLLPLILIALGLGVVLRRKKNKPQAVNFEQNTAKPTTHLAQDEQERLNQILNKEE
                     K"
     gene            491..1402
                     /gene="ccmI"
                     /locus_tag="PXD71_002249"
     CDS             491..1402
                     /gene="ccmI"
                     /locus_tag="PXD71_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HDL6292321.1"
                     /translation="MNFWLAIIFVTLIVCGVAFYPLLKKNKQIDNTKRDSLNKAFYFD
                     RLKEVENEANEGVIDDPEQTKLELQKNLLDDIPHSTQSATSEKLTLGKSWFVALILFV
                     GGISFAIYSNVGSWFSNSMLDTTHQKLDSFYERLKTEETNPLSQEEMNQFAMALRVDL
                     QSNPNDDKKWYMLGQIGMALDDGQLAHDAFSKAAQLKPDNLDYKLSHAQLLLFSEAQE
                     DKKQGETLLKEIIRQDHTNFNALSLLAFHSFEKEDFKMAAMTWGMMLKLLPDEDPSKV
                     TVERSFNMALSNLSEAEKQELQKGEKK"
     gene            1399..2004
                     /gene="mog"
                     /locus_tag="PXD71_002250"
     CDS             1399..2004
                     /gene="mog"
                     /locus_tag="PXD71_002250"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDL6292322.1"
                     /translation="MTACKPLSKEKLKIGLVSVSDRASQGVYSDQGIPELRAWLESAL
                     TEPFELETRLIPDEQAIIEQTLIELVDAHACHLVLTTGGTGPAKRDVTPDATLAVAHR
                     EMPGFGEQMRQVSLHFVPTAILSRQVGVIRHDSLILNLPGQPKAIKETLEGVKDEQGK
                     VLVKGIFSAVPYCLQLLSEIYVETHAAICESFRPKSARREN"
     gene            2014..2352
                     /gene="glnB"
                     /locus_tag="PXD71_002251"
     CDS             2014..2352
                     /gene="glnB"
                     /locus_tag="PXD71_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDL6292323.1"
                     /translation="MKKIEAIIKPFKLDDVREALTDVGVSGMSITEIKGFGRQKGHTE
                     LYRGAEYAIDFLPKVKVEIVIPDELEEQCIEAIMETAQTGKIGDGKIFVYDVGRVIRI
                     RTGEENEEAI"
     gene            2433..2912
                     /gene="lspA"
                     /locus_tag="PXD71_002252"
     CDS             2433..2912
                     /gene="lspA"
                     /locus_tag="PXD71_002252"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL6292324.1"
                     /translation="MKTGLKFLWVSLIVIIIDLWSKYIVVQHFEFGESINILPIFNFT
                     YARNYGAAFSFLADHSGWQKYFFLGLAVVISSALVVALWKNQAVKKLENIAYALIIGG
                     AIGNAIDRAYNGYVVDFLHFYWDIYHYPVFNIADIAICVGAGLLILEAFITKKEPKK"
     gene            2923..3867
                     /gene="ispH"
                     /locus_tag="PXD71_002253"
                     /gene_synonym="lytB"
     CDS             2923..3867
                     /gene="ispH"
                     /locus_tag="PXD71_002253"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDL6292325.1"
                     /translation="MKILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNKF
                     VVDGLKAKGAIFVEELHEVPDDAIVIFSAHGVSQVVRQEAKNRGLKVFDATCPLVTKV
                     HMQVARASKKGTKAILIGHEGHPEVIGTMGQYNNQEGGIYLVESVEDIANLPVSNNDE
                     LTFMTQTTLSIDDTIGVIEALKEKYPAIQGPRKNDICYATTNRQHAVRELAEQSQLVL
                     VVGSKNSSNSNRLAELASRMGVMSKLIDGPQDIDPTWLEGIETIGITAGASAPEVLVQ
                     SVISHLKTLGVTEVETLVGNEEDVVFEVPKELRIKEVK"
     gene            3867..4115
                     /locus_tag="PXD71_002254"
     CDS             3867..4115
                     /locus_tag="PXD71_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HDL6292326.1"
                     /translation="MTEINRFKLEWACRRGMRELDKMIMPFYQNHFNELNETQQQAFV
                     EMLNYSDPELFRWVMLQAPAPTEEIAELIRLMRDKIET"
     gene            complement(4322..>4839)
                     /locus_tag="PXD71_002255"
     CDS             complement(4322..>4839)
                     /locus_tag="PXD71_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6292327.1"
                     /translation="SANQYIFNSDWFTHNVPSLASIFEYVKPRRILEIGSFEGRSSVF
                     FIEEALKYQSQVEIHCIDSWEGGAEHQGVWDMGEIEQRFIHNVTLAQQKFQNVVLYKH
                     RGYSHQKMIELLANGYINYFDYIYIDGSHEAPDVLFDALLAHRLVRKGGVISFDDYLW
                     SPDETGKQRHY"
BASE COUNT         1527 a          978 c         1043 g         1291 t
ORIGIN      
        1 tcaaaattat gaaaataaaa actctcagcc taataatgct tctaagtgta acagttaatg
       61 ctgctccggt agaactgcac caattcgaca gcccgaaaca agagtcggac tatcgtgcct
      121 taattcaaga actgcgttgc ccgcagtgtc aaaataataa tatcgccgat tccaatgcga
      181 ctattgccac cgatatgcgg gcaaaaacct tagaattgct caaacaaggc aaaagcaaag
      241 acgaagtggt ggattatatg attgaacgct acggcaattt cgtcacttac gacccgcctc
      301 tcacaccggc aaccattatg ttatggctgt tgccgttaat tttaattgcc ttagggctgg
      361 gggtggtgtt gcgtagaaag aaaaacaaac cacaagcggt caattttgag caaaatactg
      421 caaaaccgac cactcatctc gctcaagatg agcaagaacg tcttaaccaa attctcaata
      481 aggaagaaaa atgaatttct ggctggcaat tattttcgtt acactgattg tatgtggcgt
      541 ggcgttttat ccgttactta aaaagaacaa gcaaatcgat aacaccaagc gagacagctt
      601 aaacaaagcc ttctattttg accgcttaaa agaagtggaa aacgaagcga atgaaggggt
      661 gattgatgac cctgaacaaa ccaaattaga gctacaaaaa aacttgcttg atgatattcc
      721 acacagcaca caaagtgcca caagtgaaaa attaacgctc ggcaagtcgt ggtttgtggc
      781 attgatttta tttgtcggcg gtatcagctt tgcgatttac agcaatgtcg gttcttggtt
      841 ctccaactca atgttagaca ccactcacca aaaattagac agcttctatg agcgtttaaa
      901 aaccgaagag accaatccgc tctcacaaga ggaaatgaac caatttgcga tggcattacg
      961 ggtagattta caaagtaatc cgaatgacga caaaaaatgg tatatgctcg gtcaaatcgg
     1021 gatggcgtta gatgatggtc agcttgcaca cgatgccttt agcaaagcgg ctcaactcaa
     1081 gccggataac ttagattaca aactcagcca cgcccaactg ctgctctttt ccgaagcaca
     1141 ggaagacaaa aaacagggtg aaaccttgtt aaaagagatt atccgccaag accacacaaa
     1201 ctttaatgcg ttaagtttat tggcgttcca ctcctttgaa aaagaagatt tcaaaatggc
     1261 ggcaatgacg tggggaatga tgttaaaact actgcctgat gaagatccta gcaaagtaac
     1321 ggtagaacgc agttttaata tggcgttatc caatttaagt gaggcggaaa aacaagaatt
     1381 gcaaaaaggt gagaaaaaat gaccgcttgt aaaccactta gcaaagagaa gttgaaaatc
     1441 ggcttggtgt cggtttcaga ccgagcctcg cagggggttt attcagacca aggcattccc
     1501 gaattaaggg cgtggcttga atctgcctta accgagccgt ttgagctgga aacccgctta
     1561 attcccgatg agcaagccat tattgagcaa accttaatcg agttagtcga tgctcacgct
     1621 tgccatttgg tattaaccac cggcggcacc ggcccggcaa aacgtgatgt caccccagat
     1681 gccacccttg ctgtcgccca ccgagaaatg cccggctttg gcgaacagat gcgacaagtg
     1741 agccttcact tcgtgccaac cgccattctc tctcgccaag tgggagtaat tcgccacgac
     1801 agtctgattt taaatctgcc ggggcaaccg aaagcaatta aagaaacgct ggaaggtgtg
     1861 aaagatgaac agggcaaagt attggttaaa ggtattttta gtgccgtgcc atactgttta
     1921 caacttttaa gcgaaattta tgtcgaaacc cacgcagcta tttgcgaaag tttcagaccc
     1981 aaatcggcaa gacgagaaaa ttaaggctaa aaaatgaaaa aaatagaagc aattattaaa
     2041 ccttttaaat tagacgatgt gcgtgaagcc ttaaccgatg tgggtgtcag cggaatgagt
     2101 attaccgaaa tcaaagggtt tggcagacaa aaaggacata ccgagcttta tcgtggggcg
     2161 gaatatgcga ttgattttct accgaaagtg aaagtcgaaa tcgtgattcc cgacgagttg
     2221 gaagaacagt gcattgaagc aattatggaa accgcccaaa ccggcaaaat cggcgatggc
     2281 aagatttttg tgtatgatgt cgggcgggtg atccgcattc gtaccggcga agaaaatgaa
     2341 gaagcgattt agttttgcaa aaaatccggc aaaaacgacc gcttgcaggg ggatttccat
     2401 ctactctttt tacattaaat tttggatatt aaatgaaaac aggcttaaaa tttctttggg
     2461 tgagtttaat tgtaatcatc attgaccttt ggtcaaaata cattgtagtg caacattttg
     2521 aatttggtga aagcattaat attttgccga tttttaactt cacttacgcc cgaaactacg
     2581 gggctgcctt tagtttcctt gccgaccatt ccggctggca aaaatatttc tttttagggc
     2641 tggcggtagt gatttccagt gctttagtgg tggctctttg gaagaatcaa gcggtcaaaa
     2701 aattagaaaa tattgcgtat gccttaatta ttggtggagc gattgggaat gccattgacc
     2761 gtgcttacaa cggctatgta gttgatttcc ttcattttta ttgggatatt tatcactacc
     2821 ctgtgtttaa tattgccgat attgcgattt gtgtgggtgc aggcttactg attttagaag
     2881 cctttataac caaaaaagaa ccgaaaaaat aagagtgatt aaatgaaaat tttattagca
     2941 aacccacgcg gtttttgtgc cggcgtggat cgagccattt cgattgttga attagcctta
     3001 gaaattcacg gtgcacctat ctatgttcgc cacgaagtgg tacataacaa atttgtggtg
     3061 gatggcttga aagccaaagg ggctattttt gttgaggagc tacacgaagt gccggacgat
     3121 gctatcgtga tcttctcagc ccacggggtg tcgcaagtag ttcgccaaga agccaaaaat
     3181 cgtggcttaa aagtgtttga tgccacttgc ccactcgtga ccaaagtgca tatgcaggtt
     3241 gcccgagcca gtaaaaaagg gacaaaagca attttaatcg gtcacgaagg tcaccctgaa
     3301 gttatcggca caatgggaca atacaataac caagaaggtg gcatttattt ggtagaatcg
     3361 gtggaagata ttgccaactt gccggtttcc aacaacgatg aattaacttt tatgacgcaa
     3421 accacacttt ccattgacga taccattggc gtgattgagg cgttaaaaga gaaatatccg
     3481 gcaattcaag gccctcgcaa aaatgatatt tgttatgcta ccaccaatcg ccaacacgca
     3541 gtgcgggaac tggcagaaca atcacaatta gtgttagtgg taggttcaaa aaactcctcg
     3601 aactcaaatc gtttggcgga actcgcctct cgaatgggtg tgatgtcaaa actgattgat
     3661 ggtccgcaag atattgatcc aacttggttg gagggtattg aaactatcgg tattaccgcc
     3721 ggtgcttccg ctcctgaagt gttggtgcaa tcagtaattt cgcatttaaa aacccttggc
     3781 gtaaccgaag ttgaaacctt agtcggcaac gaagaagacg tggtgtttga agtcccgaaa
     3841 gagttacgca tcaaagaggt aaaataatga cagaaatcaa ccgctttaaa ttagaatggg
     3901 cgtgccgacg tggtatgcgt gagttagaca aaatgattat gccgttttac caaaatcatt
     3961 ttaatgaact caatgaaacc caacagcaag ctttcgttga aatgctcaat tacagcgacc
     4021 cggaactgtt tcgctgggta atgctgcaag cacctgcccc aaccgaagaa atcgctgaat
     4081 tgattaggtt aatgcgagat aaaattgaga cctagagcta atagattaac ctcttctttg
     4141 gggtaaatat atagctactt accccaaaga agactaaatt cattcaactt ttacaaaata
     4201 accctaaact agaaaagtta atcttccaat ttaataatat gttgctgata agctattaaa
     4261 ccttgtatta catgcacttt ctgctgatag gtattgacat agtgatcaac agcgggtttt
     4321 actaataatg acgctgttta cctgtctcat ctggtgacca aaggtaatca tcaaaactaa
     4381 taacgcctcc ttttctcact aaacgatgtg caagtaaagc atcgaacaac acatctggcg
     4441 cttcgtgaga tccatctatg taaatatagt caaaataatt tatatatcca tttgcgagaa
     4501 gttcaatcat tttttggtga gaatagccac ggtgcttata taacacaaca ttttggaatt
     4561 tctgttgcgc aagagtgaca ttatggataa atcgttgttc tatttctccc atgtcccaaa
     4621 ctccttgatg ctctgctccc ccttcccaac tatcaataca atgaatttca acttgtgatt
     4681 gatatttcaa tgcttcttca atgaaaaaga cagatgatct accttcaaat gatccaattt
     4741 ctaaaatcct ccgaggtttt acatattcaa aaatagaagc taatgaagga acattatgag
     4801 taaaccaatc agagttaaaa atatattggt tggctgact
//
LOCUS       DANLXS010000179         4751 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.180, whole genome shotgun sequence.
ACCESSION   DANLXS010000179 DANLXS010000000
VERSION     DANLXS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.180"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            136..831
                     /locus_tag="PXD71_002256"
     CDS             136..831
                     /locus_tag="PXD71_002256"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDL6292328.1"
                     /translation="MARNIIGILGGMGPAATADMFQKFIRLTPADCDQAHIPLLISSI
                     PDIPDRTRYILANGENPAPVMEKYIEGLESAGATCIIIACNTAHYWFEHLKAKSQVEM
                     LSMIDCVVDEVLKCGKWRVGLLATDATLAVDLYKKQLEAQGLTFICPTENGQKAVMDS
                     IYLLKSGELEKATELMLQQRDELIRLGAEVIILGCTEVPIILAQEIEREPMRYVDSTL
                     VLVKKAIDWYQTH"
     gene            847..2157
                     /locus_tag="PXD71_002257"
     CDS             847..2157
                     /locus_tag="PXD71_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL6292329.1"
                     /translation="MIYAEIFVVLAAIYLGLRSGGLGIGLYGGLGLAILTLGFGLPMG
                     SIPVDVMLIIMTVVIGAAVLQAVGGMDLLVRYAEVLMRKNPKYINFIAPMITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRALAGSVVSSQLAIAGSPLSAAMAAMIAVMEN
                     NSVTFFQVIAVCLPVSFIASMVAAFVASKQGCELQNDVVYLERLKAGIVSPSRKLASE
                     STYSKGAKLSIGIFLLATVLVVLLAAVPGLKPVYENGKSMSTRDIIIVMMLASSCLMM
                     LVGKIKPDTIVASPIFRSGMTSIAVIIGIVTLGMTFVDAHLIEIKASIGNILTEYPML
                     LAVVLFFTCALLYSQGSTSALIIPLAVSLGIPNWAILASFIAITGIFILPTYPTSLAA
                     MELDTTGSTRAGKYIIDHPFMLPALVGVAVGLAFGFVWAPMIVS"
     gene            2335..3786
                     /locus_tag="PXD71_002258"
     CDS             2335..3786
                     /locus_tag="PXD71_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL6292330.1"
                     /translation="MPNHKFLLLRFQAYINGKFVSSQNGKTFTVQNPTNGEKIGEVAD
                     LGTEETAQAIEAAAVAQKKWQALLPKERSVILRKWFELIMQNQEELAQILSLEQGKPL
                     AESRGEIAYGASFIEWFAEESKRIYGDVLPQDKAGRRLFVTKQPIGVVAAITPWNFPN
                     AMITRKAAPALAAGCAIVIKPAAETPLSALALAQLADQAGIPKGVFNIITSSDVEGVG
                     RELSTNPTVRKLTFTGSTRVGKILMQQSAGTVKKLALELGGNAPAIVFDDADLDTAVE
                     GVFASKFRNGGQTCVCANRIYVQAGIYEQFLAKLSEKVAQIKLGSPFEEEVTIGALIS
                     ERAVEKVESHINDALSLGAELVLGGERHALGGTFFQPTILKNVTQAMKVAREETFAPL
                     APIFKFETEEEVIQWANDTEFGLAAYLFSQNIGRIWRVSEQLEYGIVGINEGIISNEI
                     APFGGWKESGLGREGSKYGIEEFLEIKYLCMKV"
     gene            complement(3862..4686)
                     /gene="iolB"
                     /locus_tag="PXD71_002259"
     CDS             complement(3862..4686)
                     /gene="iolB"
                     /locus_tag="PXD71_002259"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL6292331.1"
                     /translation="MSSPLLSKPNFNQHEGLIQHITPENAQWEYVGFDVYQLSKQQIT
                     LESGENELCLVFVTGFGSVYVDDYAFENIGNRTTPFEKIPPYAVYIPPHKSVLVKTEL
                     NLELAVCKAPSQGNYPVRLISPNHIGVEKRGFGNNQRLVHNILPETEAADSLLVVEVF
                     TDEGNTSSYPSHKHDDKHSTEETYLEETYYHRFDPPQGFAMQRVYTDDRTLDECMAVY
                     DKNVVKVPRGYHPVATIAGYNNYYLNVMAGPVRKWKFTWEQDHLWVNSETYKAKFE"
BASE COUNT         1329 a          867 c         1096 g         1459 t
ORIGIN      
        1 tttaaagatt ttttttctat ctataaagag ctttgttgca ttttttgcat actgattcga
       61 tttattgcat ttcttttttg cataacttgt ttgtataagt catcgcagta aattaaatct
      121 tataaggaga agtgtatggc aagaaatatt attggcattt taggtggaat gggacctgca
      181 gcaacagcag atatgttcca aaaatttatc cgcttaacac cagctgattg tgatcaagct
      241 catattccat tattgatttc ctctattccc gacataccag atagaactcg ctatatttta
      301 gcaaatggag agaatccggc tccggtaatg gaaaaatata ttgaagggct tgagagtgca
      361 ggggcaacct gcatcattat tgcatgtaat actgcacatt attggtttga acacttgaag
      421 gccaaaagtc aggtagagat gctaagtatg attgattgtg tggttgatga ggttttgaag
      481 tgtggaaaat ggcgggttgg attgttggca actgatgcaa ctttggcggt agacctatac
      541 aaaaaacagc ttgaagcaca aggactgact tttatttgcc cgacagaaaa tggtcaaaag
      601 gcagtaatgg atagtatcta tttattaaaa tcaggtgagt tagagaaagc cacagagcta
      661 atgttacaac aacgtgatga actcattcgt ttaggtgcag aagtaattat tttaggttgt
      721 acagaagtac ctattatttt agctcaagaa atagagcgtg agccaatgcg ttatgtagat
      781 tcaacgttgg tattggttaa aaaagcaatt gattggtatc aaactcatta atttaaggag
      841 atggatatga tttatgcaga aatctttgtg gtacttgctg caatctattt aggactgcgt
      901 tctgggggct taggaatagg actatatggt ggtttagggc ttgcgattct aactctaggt
      961 tttggtttac ctatgggttc tattccagtt gatgtaatgc tgattattat gactgttgta
     1021 ataggtgctg ctgtgttaca agcagtagga ggtatggatt tacttgttcg ttatgcggaa
     1081 gtattgatgc gtaaaaatcc taaatatatt aactttattg caccgatgat tacttggtta
     1141 atgacgatta tggctggtac aggttttatc gtcttttcca ccttacctgt gattgcagaa
     1201 gtggcaaaag agagtggtat tcgcccttca agagcattag ccggctcggt tgtgtcttct
     1261 caattagcta ttgcaggttc tccattgagt gctgcaatgg cggctatgat agcagtaatg
     1321 gaaaacaaca gtgttacatt ttttcaagta atagcagtat gcttacctgt ttcttttatt
     1381 gcctctatgg ttgctgcatt tgttgcttct aagcagggct gtgaattgca aaatgatgtt
     1441 gtttatttag aacgtttaaa agcaggaatc gtcagcccat ctcgtaagtt agcgagtgaa
     1501 tctacctatt caaaaggagc aaaactatcg attggcattt tcttactagc aactgtattg
     1561 gttgttctac ttgcagccgt tccagggtta aagccagtat atgaaaatgg gaagtcaatg
     1621 agtacccgag atataattat tgtgatgatg ttagcctctt cgtgcttaat gatgttggta
     1681 ggaaaaatta aacctgatac gattgtggct agtccaattt tccgctcagg aatgacttct
     1741 attgcggtaa ttatcgggat tgttacatta ggtatgactt ttgtcgatgc acatttaata
     1801 gaaattaaag cctctattgg aaatatttta actgaatatc caatgttgtt agctgtagta
     1861 ctctttttta cttgtgcatt actttattca caagggtcaa cttctgctct tattattcca
     1921 ttagcagtaa gcttggggat ccctaactgg gcaattttgg cttctttcat tgcaattaca
     1981 ggcattttta tcctgccaac ctacccaact tcattagcag cgatggaatt agatactacg
     2041 ggttcaactc gtgctggcaa atatattata gatcatccgt ttatgcttcc agctttagtt
     2101 ggggttgctg ttgggcttgc ttttggattc gtatgggctc caatgattgt atcttaatta
     2161 ttaaatatgt agagaattta agttaattct caataataaa tggctgcacc ttagaagaac
     2221 gcttgtctaa ggtgctttat tattgagtaa aaagttattt ttcagctctt ttctctttac
     2281 tgaaattgga ttatagtcaa tattgttctc tttttaacca ttcaaggagt gattatgcca
     2341 aatcacaaat ttttgctact ccgtttccaa gcctatatta acggtaaatt tgtttcaagc
     2401 caaaatggga aaacctttac ggtacagaat ccgactaatg gtgagaaaat cggtgaggtg
     2461 gctgatttag gcacggaaga aaccgctcaa gcgattgaag cagccgcagt tgcccagaaa
     2521 aaatggcagg cattattgcc caaagagcgt tccgtgattt tgcgtaaatg gtttgagctg
     2581 attatgcaaa accaagaaga actcgcccag attttaagtc tagaacaggg taaaccattg
     2641 gcagaaagtc gaggcgagat tgcctatgga gccagtttta tcgagtggtt tgccgaagaa
     2701 agcaagcgga tttatggcga tgtcttgcca caagataaag ccggtcgccg cttgtttgtg
     2761 actaaacaac ccattggcgt ggtggcagca attacaccgt ggaatttccc aaatgcaatg
     2821 attacccgta aagcggcacc ggcgttagcg gcaggttgtg cgattgtgat taaaccggcg
     2881 gcagaaaccc cgctttctgc cttagcatta gcccaattag cggatcaagc aggtattccc
     2941 aaaggggtat ttaacattat taccagtagc gatgtcgaag gtgtagggcg ggagttgagc
     3001 actaatccga ccgtacgcaa actcaccttt accggctcca cccgtgtcgg caaaatttta
     3061 atgcaacaat cggcagggac ggtaaaaaaa ttggcattgg agctaggcgg taatgcaccg
     3121 gcgattgtgt ttgatgatgc ggatctagat acagcggttg agggcgtgtt tgcctctaaa
     3181 ttccgtaacg gtgggcaaac ctgcgtctgt gctaaccgaa tttatgtgca agcgggcatt
     3241 tatgagcaat ttcttgcaaa attgagtgag aaagtggctc aaatcaagct tggctcgcca
     3301 tttgaggaag aggtcacgat aggtgcacta attagtgaac gagcggtgga aaaagtcgaa
     3361 agccacatta acgatgccct ttcacttggg gcagaactgg tgttaggcgg tgaacgccac
     3421 gcactgggtg gcacattctt ccagccaacc attctgaaaa atgtcactca agcgatgaaa
     3481 gtggctcgtg aggaaacctt tgctcccctt gccccgattt tcaaatttga aaccgaagaa
     3541 gaggttatcc agtgggcgaa tgatactgag tttggtttgg cggcttactt attcagccaa
     3601 aatatcgggc gaatttggcg agtatcggag cagttggaat atggcattgt cgggattaat
     3661 gaggggatca tcagtaacga aatcgctccg ttcggcggct ggaaagagtc tggacttggg
     3721 cgggaaggtt cgaagtacgg tattgaagag tttttagaaa taaaatatct ttgtatgaaa
     3781 gtataaccga atgggacgcc aaaagcgtcc catttttaat cccttatttt gtaaaaaatt
     3841 ggtggaaaaa gaccgcttgc attactcaaa tttggcttta taggtttcac tgttcaccca
     3901 aagatgatct tgttcccaag taaatttcca tttgcgtacc ggccctgcca taacatttag
     3961 gtagtagttg ttatagccgg caatggtagc aacagggtgg tagccacgag gcactttaac
     4021 cacattttta tcatacaccg ccatacattc atcaagtgtg cgatcatcgg tataaacccg
     4081 ttgcattgca aaaccttgtg gtggatcgaa gcggtggtag taggtctctt ccaaataggt
     4141 ttcttcggta gagtgtttgt catcgtgctt gtggcttggg taggaactgg tattgccttc
     4201 atcggtaaag acttccacca ccagcaaact gtcggctgct tcggtttccg gcagaatatt
     4261 atgcaccaag cgttgattgt tgccaaagcc ccgtttttcc acaccgatat ggttcgggct
     4321 aatcaaacgc accggataat taccctgact tggtgctttg cacactgcca attctaaatt
     4381 gagttcggtt ttaaccaaca cgcttttgtg tggcgggata tagaccgcat aaggcggaat
     4441 tttttcaaat ggcgtggtac ggttgccgat attttcaaaa gcataatcat caacatagac
     4501 agagccaaaa ccggtcacaa agactagaca aagttcattt tcaccacttt ccagcgtaat
     4561 ttgttgctta ctgagctgat aaacatcaaa tccaacatat tcccactgtg cattttcagg
     4621 ggtaatgtgc tgaattaacc cttcatgttg gttgaaattg ggtttagaca ataatggact
     4681 agacatagga aacctcgctt aacgaaatgg aatacgcttt caaactatac gaaattttgt
     4741 ctcattttaa t
//
LOCUS       DANLXS010000180         4743 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.181, whole genome shotgun sequence.
ACCESSION   DANLXS010000180 DANLXS010000000
VERSION     DANLXS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.181"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..564)
                     /locus_tag="PXD71_002260"
     CDS             complement(<1..564)
                     /locus_tag="PXD71_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292332.1"
                     /translation="MEGLIMGLQLIPHFPELAISRRTYLHDMPMYCFVSKNWIFSAKD
                     RAKREALGNLLACGGEMMKSFIFKRSLFSEIWFEDILLEDEEKELIRILYNKPEPEYQ
                     VEEKIDWEAREARRKQADYDKWQSYDERNPFHYQIFPTDTIAYAQQQVEKFIEDVDNF
                     LEWFYDDEVKRYILERNEIYQKKFDLVN"
     gene            complement(1097..1423)
                     /locus_tag="PXD71_002261"
     CDS             complement(1097..1423)
                     /locus_tag="PXD71_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6292333.1"
                     /translation="MLHSKGKILPKITLNEIETKERFAIALRKEMHAQYWTGKMVANA
                     LDVSEKTVKDWVAGKRLPSSLDLLALMRISSCVRQLVWDISKSEECYQDAEMLKQICF
                     VLLSNL"
     gene            1471..3102
                     /locus_tag="PXD71_002262"
     CDS             1471..3102
                     /locus_tag="PXD71_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL6292334.1"
                     /translation="MVTRTKNQPKNQAVSYIRMSTEHQEFSPDLQRCFIENYAKENGL
                     EIIREYIDEGKSGLTAEHRPQFLSMIQLVQSGQADFDHILVYDVSRWGRFMNIDESGH
                     YELICTYQGIKVHYCAEPFKGNDIASQIYKTMKRLSVGDYCRELGVKVFNGQKNLIQH
                     GFRQGGVAGFGLRRRLIDCQGNPKFDLQRGDRKSLQTDRVILVAGPKEEQEIVRQIYH
                     DFVYQHKTEQQIADSLNAQGIVTDRNTAWTKGVVHQILINEKYVGHNVWNKRSASKTR
                     EYKGKNPVEEWVRAENVFEAIVPQALFNAAQTIIHQRSARLSDDEMLEQLKLLLKEKG
                     KLSGLIIDEAENCPSSSAYSSRFGSLLNTYTLINYEPERDYHYVEINRLLRQQHKNFV
                     QQTLDTIIELGGVVLSDDVTDLVHINNEFTASIVLSRCRLTPSGSKRWLIRFDTSLNP
                     DITIAVRINESATEILDYYLLPTTEKVNEKLRLAESNPAELEIYRHDNLDRFFIMVER
                     ILVKEFIYAKRHHSNSSYRQNSGTQSQNAQSESLC"
     gene            3017..3910
                     /locus_tag="PXD71_002263"
     CDS             3017..3910
                     /locus_tag="PXD71_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL6292335.1"
                     /translation="MQNDTIQILPIDKIRVLNPRTRNQKVFADLVENIATIGLKRPIT
                     VTPSVDSAEYYDLLCGQGRLEACQVLGELFIPCRVVNANSEESYLISLVENIARRQHS
                     YMELLSGIKILNDRGYKPVDIARKIGFSKSHVNSILHLINAGEERLINAVEQGILPIT
                     IAISISRSDDQEVQKQLAELYTNGTLKSTDIAKIRNLIHRRNLAGKKANTGISGSIRS
                     SIYNPKTVVNIYKEETERQKMMIKQADYDEKQLSIILSCLNKLFEDKYFQLVLKSEHL
                     DDMPKDLSQRMLKQREGNIYA"
     gene            3903..>4743
                     /locus_tag="PXD71_002264"
     CDS             3903..>4743
                     /locus_tag="PXD71_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL6292336.1"
                     /translation="MPKHITYAFHNQLISLPIDEIIPLKKMDKNFEKSTKFQSILASI
                     REIGIVEPVVVYPEENNQYILLDGHSRVHALKKLGVDKVLCMISTDDEGFTYNKQINR
                     LTAVQENKMLMKTIERGVSDELIARTLNINLKALRQKMNMLNGITREVVDILANKQVS
                     KEIFRILRKMKPERQIEVAQMMIASNKFTLTYANMMLLSSRKEELVESHKSKTTNDDL
                     TDLSIMQKELERLKENYKISEEKLADLKMGLVVAKGYVKKLLNNAAVVDLLENDQSEI
                     YLTL"
BASE COUNT         1551 a          916 c          926 g         1350 t
ORIGIN      
        1 gttcactaaa tcgaatttct tttggtaaat ctcattcctt tctaatatat agcgttttac
       61 ctcatcatcg taaaaccatt caagaaagtt atcaacatct tcaatgaact tttctacctg
      121 ttgctgtgca tatgctatcg tatcggtagg gaagatttga taatgaaacg gattgcgttc
      181 atcataactc tgccatttgt cataatcggc ttgtttacgc cgcgcttctc ttgcctccca
      241 gtcgattttt tcttccactt gatattctgg ctctggctta ttatacaaaa tacggatgag
      301 ttccttttct tcatcctcaa gcagaatatc ttcaaaccag atttcagaaa ataggctgcg
      361 cttgaaaatg aaagacttca tcatttcccc tccacacgcc aataagtttc ccaatgcttc
      421 ccgtttcgct ctatcttttg ccgaaaatat ccagtttttc gatacaaaac agtacattgg
      481 catgtcatgc aaataggttc ttcgtgaaat ggctaattca ggaaaatgag gaataagctg
      541 taaccccatt attaagcctt ccatactgca cacgatctta cttaattgtc cgtaatcgcg
      601 taaaaaagca atgtcagttt ttatcaacca caacgatagg ctgactaata tgaacataaa
      661 gatgagataa tagatcggtt ttaaggctaa ggcgcgccga caaagccaca ttaagagtgg
      721 aatcgtaacg agatcaaaga aaaatgatga gtctatgatg atttccttat gtgagttgat
      781 gaataatcag gcatgaagga aaccttaaaa aggaaaagtc ccttcattac cactttttaa
      841 aaccttttaa ggcttaaaaa tcaaaagtcg taatgaaggg actattgtcc gtgggcaaaa
      901 aggtattagt tatagggggc tttgtcaagc gaaaatttac tgtgcatttt ctcgccaaaa
      961 ctgatttaag ccttgataaa atctcgaaag taccttgtca agaaaaatct ttttagtggt
     1021 ttgattgcta gttttttgag caaaactatg ctgtaatgca gtaaaaatac agtttctatc
     1081 tgtgtttgga atccagttac aaattggaga gtaatacaaa gcatatttgc ttgagcatct
     1141 cagcatcttg gtagcattct tctgattttg aaatatccca aacaagctgc cgtacacatg
     1201 atgaaattct cattaacgcc aataaatcta ggctacttgg caatcttttc cctgcaaccc
     1261 aatctttaac tgttttctca ctcacatcta atgcattggc gaccattttc cccgtccaat
     1321 attgagcatg catttccttt cgtaacgcaa ttgcaaaccg ctctttcgtt tcaatctcat
     1381 tgagtgtaat tttgggtaag attttaccct ttgagtgcaa catatttcct cccgattcct
     1441 ttatattggt gtagataagg agtcattaga atggttacac gtacaaagaa tcaacctaaa
     1501 aatcaggcgg tttcctacat tcgaatgtca acggaacacc aagaattttc accagacctt
     1561 caacgttgct ttatcgaaaa ttatgctaaa gaaaatgggc tagaaatcat tagagaatac
     1621 attgatgaag gaaaaagtgg gctaaccgca gaacaccgcc cacaattcct ttcaatgata
     1681 caactcgtac aaagtggaca agccgacttc gatcatattt tggtgtatga cgtgagtcgt
     1741 tggggtcggt ttatgaatat tgatgaatcg gggcattatg aactgatttg tacctaccaa
     1801 ggcattaaag tccactattg cgccgagcca ttcaaaggga atgatattgc ctctcaaata
     1861 tataaaacca tgaaacgttt gtcggtagga gactactgtc gtgaactcgg agtgaaagta
     1921 tttaacggtc aaaaaaatct gatccaacat ggatttagac aaggtggggt agctggattt
     1981 ggattgcgta ggagattgat tgactgtcaa ggaaacccta aatttgatct ccaacgtgga
     2041 gatagaaaga gcctacaaac cgacagagtg attttggttg caggcccaaa ggaagagcag
     2101 gaaattgtcc gacaaattta ccatgatttt gtttatcaac ataaaacgga acaacaaatt
     2161 gctgatagct taaatgcaca aggcattgtt actgatagaa atacagcatg gacgaaaggc
     2221 gtagtacatc aaatccttat taacgaaaag tatgtcggcc acaatgtttg gaataaacgt
     2281 agtgcatcaa aaacacgcga atataaaggt aaaaaccccg tagaagagtg ggtgcgagct
     2341 gagaatgtat ttgaagctat cgtgccacaa gccttattta atgcagccca aaccattatt
     2401 catcaacgct ccgcccgatt atctgacgat gaaatgttag agcaactcaa gctacttttg
     2461 aaagagaaag ggaagctgtc tggcttgatc attgatgaag ccgaaaattg tccatcaagt
     2521 agcgcgtaca gtagccgatt tggaagccta cttaacacat atactttaat taactatgaa
     2581 cccgaacggg attaccacta tgtagagatc aaccgattac tccgtcagca gcataaaaat
     2641 ttcgtacaac aaacacttga taccattatt gaattggggg gagtggtttt gagcgatgat
     2701 gtaaccgatt tagtccacat caataatgaa tttaccgcct caatcgtatt aagtcgttgt
     2761 cgcctaacgc catcaggaag caaaagatgg ttgatccgat ttgataccag tttaaatccc
     2821 gacataacaa ttgcagtgcg catcaatgag agtgccacag aaattcttga ttactacctg
     2881 cttcccacaa ctgaaaaggt aaatgaaaaa ctacgattgg ctgagagtaa tcctgccgaa
     2941 cttgaaatct accgccatga taatttagat cgctttttta ttatggttga acgtatttta
     3001 gtcaaggagt ttatctatgc aaaacgacac cattcaaatt cttcctatcg acaaaattcg
     3061 ggtactcaat cccagaacgc gcaatcagaa agtctttgct gatctggtcg aaaacattgc
     3121 aacgattggt ctcaagagac ctattaccgt aacgccatct gtcgatagtg ctgaatatta
     3181 tgatttgtta tgcggtcaag gtcggcttga ggcttgtcag gtgcttggtg agctattcat
     3241 accttgtcgc gttgtgaacg caaatagtga agaaagctac ttgatcagcc ttgtggaaaa
     3301 tattgccaga cgtcagcatt cctatatgga gctgctttca ggcatcaaaa tactcaatga
     3361 tagaggttat aagcccgttg atattgctcg caaaatcggt ttctcaaaaa gccatgtcaa
     3421 cagtatctta cacctcatca atgctgggga agagcgcctt atcaatgcgg tagagcaagg
     3481 aattttaccc attaccatcg ctatttcgat ttctcgttca gacgatcagg aagttcaaaa
     3541 acaactcgcg gaactttaca ccaatggtac attgaaaagc accgatattg ccaaaatacg
     3601 taacctgatc caccgtagaa atcttgctgg taagaaagcc aatacaggta tttctggaag
     3661 tattagatca tctatttata atcccaaaac tgtggtgaat atctacaaag aggaaacgga
     3721 aagacagaaa atgatgatca agcaagcgga ctatgatgaa aaacaactct ctattatctt
     3781 gtcttgctta aataaactct ttgaggacaa atactttcaa ctcgtcctta aatcagaaca
     3841 tttagacgat atgccaaaag atttaagcca acgaatgtta aaacaacgag agggaaatat
     3901 ttatgcctaa acatattact tatgcctttc ataatcagtt aatttcgctt ccaattgatg
     3961 agattattcc attaaaaaag atggataaaa attttgagaa aagcactaag ttccaaagca
     4021 ttctggcttc aattcgtgaa atcgggattg ttgaacctgt ggtcgtctat ccagaagaaa
     4081 acaaccaata tattttgtta gatggccatt ctcgtgtaca tgcactcaaa aagctaggtg
     4141 tggataaggt gctttgtatg atttctaccg atgatgaagg ctttacttac aacaagcaaa
     4201 ttaaccgctt aaccgccgtt caggaaaata aaatgctgat gaaaaccatt gagcgcgggg
     4261 tttcggatga actgatcgca agaacactca atatcaatct aaaagcacta cggcagaaaa
     4321 tgaatatgct taatgggatc acaagagagg ttgttgatat attggccaat aagcaggtca
     4381 gtaaagagat atttcggatt ttgcgcaaga tgaagcctga acgccaaatt gaagtggcac
     4441 aaatgatgat cgccagtaat aagtttacat tgacatacgc caatatgatg ttgctcagtt
     4501 cacgcaagga ggaattagtc gaaagccaca agtctaaaac cacgaatgat gatttaaccg
     4561 atctttccat tatgcaaaaa gagctagagc gacttaaaga gaactataaa atttccgaag
     4621 agaaattggc ggacttaaaa atgggattgg ttgtggcgaa agggtatgtc aaaaaactat
     4681 tgaataatgc tgccgtggta gatttgcttg aaaatgatca gagtgaaatc tatcttactc
     4741 tta
//
LOCUS       DANLXS010000181         4655 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.182, whole genome shotgun sequence.
ACCESSION   DANLXS010000181 DANLXS010000000
VERSION     DANLXS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.182"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            12..2159
                     /locus_tag="PXD71_002265"
     CDS             12..2159
                     /locus_tag="PXD71_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6292337.1"
                     /translation="MKRLRYSYLALILGSALPLSAYADGYLSNDNIRQFDARFNFFLA
                     NPLPAPIAKADGRGEAIENLVVKYASDYLNDIYLKEDVIRFRRWQWQDLYTSLDLKTK
                     QKPDYLNTYRLQAEAYFINKQYQEALSQLDQILRRNPNDVHAMAMSVVASRVLENPNT
                     EAERLLALYQISPSIAQKVQQYLHFTDTMLAASYGSEPQTTMIPDTIAVFGQSPNPDG
                     TPSKGLLQRLEKTKEMAEKFPNAKIILSGGPVRYIYAEADVMKKWLIENGVDESRLLL
                     DDVARDTPGNAVGIIDFMKEYGGRKVLGIGTILHLPRAMSVLKSYADSIGYELEIDSA
                     GGGSPPNEKNKKGEALYTFVNVARAMGLFTLGDFENLLAKEQAEKEQREALEKAQQNY
                     LEKLVAERDQAVKNAENSANELEVARQALETAKQAHQQAEEELNKELSKTQEQLKQST
                     EALSRLIIKQNNPFSEHYVVQLNLMEQATYDLYRRLAEIEHTQDWNLWLSDSLSKYRL
                     KNRSTEDYHLLHIGLDHQVSHNWSIGGVFGISQGQIENRTHKVKGTSAGIYAGYRPTS
                     GLFWTNFFNYSHFTNQSGVTKRDNNAYSLSSEIGRNIVMSHQWQITPKLQLGWAKAGS
                     ARMFWAGTGLRLGKVIELNAIKLKPYADISYRFRNRHIDTLDSLSDRHRNVISIGIQA
                     VGNRHYFAIEGSMLHGDALKRSYGLHANYHYQW"
     gene            2172..3290
                     /locus_tag="PXD71_002266"
     CDS             2172..3290
                     /locus_tag="PXD71_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL6292338.1"
                     /translation="MKKIKTTLSALALSLSLISVNSWAIPADYLSQDNISQYDKAFNF
                     VLAKVLPAPKGKEDGRGAPIEHLVIQYAPDYVDDPYLKEDVLNFRRWYWQAMYASLDQ
                     KVKEKPTYLNTYRLQAEAYFVNKEYREALSQLDQILRQNPRDIHALAMTVLASRVLED
                     TEQETARLSALKQLAPEVAQRMTDFFAFTEQTLAASYGNEPQTAMIPDTIAVFGSTPN
                     KDGTPSPLLLDRLQKTKEMAQKFPQAKIIVSGGAVKTEFAEADVMAKWLSENGIEKDR
                     LLLDPVARDTPGNAVGIIKLMQTYNGKKVLGIGTLQHIPRATAVLKGYAQHIGYPIEM
                     DSVGGGKPATPGKAKIEALYTYVNVARSMGLFELGDFK"
     gene            complement(3351..4613)
                     /gene="rho"
                     /locus_tag="PXD71_002267"
     CDS             complement(3351..4613)
                     /gene="rho"
                     /locus_tag="PXD71_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL6292339.1"
                     /translation="MHLTQLKNTPVSKLVEMGENQMGLENLARLRKQDIVFAILKQHA
                     KSGEDIFGQGVLEILPDGFGFLRSSDSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKI
                     RPPKEGERYFALLKVDSVNDDKPEASRSKILFENLTPLHANSRLKMERGNGSKEDLTA
                     RILDLAAPIGKGQRALIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVT
                     EMQRTVRGEVIASTFDEPATRHVQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYN
                     TVTPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVDTGSKMDEVIFE
                     EFKGTGNMELHLSRRIAERRIFPAIDFNRSGTRKDDLLMAADELQKTWMLRKVLNPMD
                     EVDAIEWLIDKLMMAKTNEEFFEIMKRS"
BASE COUNT         1392 a          871 c         1056 g         1336 t
ORIGIN      
        1 gagaaatcat aatgaaacgc ttgcgttatt cttatcttgc tttgatatta ggttcagcct
       61 tacctttatc ggcttatgct gacggttatt taagtaacga taatattcgg caatttgatg
      121 cccgttttaa ttttttcctc gcaaatccac tacctgcgcc tattgcaaaa gcagatgggc
      181 gtggtgaagc gattgagaat ttggtggtga aatatgcatc agattatctt aatgatatct
      241 atcttaaaga ggatgttatt cgttttcgcc gttggcagtg gcaagatctc tatacgtcat
      301 tggatttaaa aacgaaacaa aagcccgatt atttaaatac ttaccgatta caggcggaag
      361 cgtactttat caataagcaa taccaagaag cattatctca actggatcaa attctgcggc
      421 gtaaccctaa tgatgttcat gcaatggcaa tgagtgtggt tgcaagtcgg gtattggaaa
      481 atcctaatac tgaagccgag cgtttattag cgctttatca aatctctcca agtattgcgc
      541 aaaaggtgca gcaatatcta cattttacag acactatgct tgctgccagc tatggtagtg
      601 agcctcaaac aacaatgatc cccgacacca tagcggtatt tgggcaatcg cctaacccag
      661 acggtactcc aagcaaggga ttattgcaac gtcttgagaa aaccaaagaa atggcagaga
      721 aatttccgaa tgcaaaaatt attttaagtg gtggacctgt tcgttatatc tatgcagaag
      781 cggatgtaat gaaaaaatgg ttgatagaga atggcgttga tgaaagcaga ctgttgcttg
      841 atgatgtggc acgagatact ccgggaaatg ctgtcggtat cattgatttt atgaaggaat
      901 atggaggtag aaaagtattg gggatcggta cgatcttaca tttaccgaga gcaatgtctg
      961 tattgaaaag ctatgcggat tccattggat atgaattgga aattgattct gccggaggcg
     1021 gttctcctcc taatgaaaaa aataaaaaag gtgaagctct ttatacattt gttaatgttg
     1081 ctcgtgcaat ggggttattt accttgggcg attttgagaa tttgcttgcc aaagaacaag
     1141 ctgaaaaaga acaaagagaa gcgttggaaa aagcacagca gaattattta gaaaaattgg
     1201 tagctgagcg agatcaagca gtaaaaaatg cagaaaattc tgcaaatgag ctggaagtag
     1261 ccagacaggc gttagaaaca gcaaaacagg ctcatcagca agctgaagag gaattgaata
     1321 aagaacttag taaaacgcaa gagcagttaa aacagagtac ggaagccctt agtcgtttaa
     1381 taataaagca gaacaatcct ttttccgagc attatgttgt acagcttaat ttgatggaac
     1441 aagcaactta tgatctgtac cgtaggttag cagaaataga acatacacaa gattggaatt
     1501 tatggctttc ggatagtcta tccaaatatc gtttaaaaaa tcgttcaacc gaagactatc
     1561 acttattaca tatcgggcta gatcatcagg tttcccataa ttggtctatt ggtggagtat
     1621 ttggtatcag tcaggggcaa attgagaata ggacgcataa agtgaaaggg acatctgctg
     1681 gtatttatgc tggttatcgt cctacaagtg gtctgttttg gacaaatttt tttaattaca
     1741 gtcattttac taatcaatct ggtgtaacaa aacgagataa taatgcatac agtctgagta
     1801 gtgaaattgg aagaaatatc gtgatgagtc atcaatggca aattacgcca aagctgcaat
     1861 taggctgggc aaaagctgga tcagcacgaa tgttttgggc tgggacaggg ctacgtttag
     1921 gaaaagtgat tgaactcaat gcaatcaaac ttaaacctta tgctgatatt agctatcgtt
     1981 ttcgaaatcg tcatattgat accttagata gtttatctga tcgtcatcgg aatgtaatca
     2041 gtattgggat acaagcagtt ggaaatcgcc attattttgc aatcgaagga tcaatgttac
     2101 acggggatgc attgaagcgt tcatacggat tacacgcaaa ttatcactat caatggtaaa
     2161 taggagcctc tatgaaaaaa atcaaaacaa ccctttctgc ccttgcttta agcctttctt
     2221 tgataagtgt aaatagctgg gcgataccag ctgattattt gagccaagac aatatcagtc
     2281 aatatgataa ggcgtttaac tttgtattag caaaagtact gcctgcacca aaaggtaaag
     2341 aagatggtcg aggagcgccg attgagcatt tagttattca atatgcgcca gactatgttg
     2401 atgaccctta tctaaaagag gatgtgctga atttccgccg ttggtattgg caagcgatgt
     2461 atgcttcgct tgatcagaaa gttaaagaga agccgaccta ccttaacact taccgtttac
     2521 aagcagaggc gtatttcgtc aataaagagt atcgggaagc actttctcag ctcgaccaaa
     2581 ttttgcgcca aaacccccgt gatattcacg ctcttgcgat gacagtatta gcttcaaggg
     2641 tgctagaaga tactgaacaa gaaacggctc gtctttctgc actgaaacag cttgctcccg
     2701 aagtcgcaca acgaatgaca gacttttttg cctttaccga acaaacgctg gcggcaagct
     2761 acggtaatga accacagaca gcaatgatcc ctgatacgat tgccgttttt ggttctacgc
     2821 caaacaaaga cggcacacct tcgccgctgt tgttagatcg attacagaaa accaaagaga
     2881 tggcacaaaa atttccgcaa gcaaaaatta tcgtcagtgg cggtgctgtg aaaacggaat
     2941 ttgctgaggc tgatgtgatg gcaaaatggc ttagcgaaaa tggtattgaa aaagaccgtt
     3001 tgctacttga tcctgttgcc agagataccc cgggaaatgc ggttgggatt atcaagctga
     3061 tgcaaactta taatggcaag aaagtattag gtatcggtac gttacaacat atccctcgtg
     3121 caacagcggt tctcaaaggt tatgcacagc acattggtta tccgattgag atggattccg
     3181 ttggtggtgg caagccggca acaccgggaa aagccaaaat cgaggcactt tatacttatg
     3241 taaatgtggc gagatcaatg gggttgtttg agttggggga ttttaagtag ttactttatt
     3301 gacagctgct attgataaaa atcccgcgtt aagcgggatt tttttatgtt ctacgaacgt
     3361 ttcataatct cgaaaaactc ttcgttagtt tttgccatca ttaatttgtc gattagccat
     3421 tcgatagcat ctacttcgtc cattgggttg aggactttgc gtaacatcca agttttttgg
     3481 agttcgtctg ctgccattaa gaggtcatct ttacgagtac ctgagcggtt gaagtcaata
     3541 gccgggaaga tacggcgttc agcaatacgg cgtgagaggt gtaattccat attacccgta
     3601 cctttgaact cctcgaaaat cacttcgtcc atttttgatc cggtatccac aagtgcggtt
     3661 gcgataatgg ttaagctgcc accttcttcc acattacggg ctgcaccgaa gaaacgtttt
     3721 ggacgatgta aggcgttagc gtccacacca ccggaaagga ttttgccgga aaccggggta
     3781 acggtgttgt aggcacgggc taaacgagtg atggagtcta ataaaatcac cacatctttt
     3841 ttatgctcta ccgagcgttt tgctttctcg attaccatct ccgcaacttg aacgtggcgg
     3901 gttgccggtt catcaaaggt tgaggcaatt acttcaccac gtacggtgcg ttgcatctct
     3961 gttacttctt ccggacgctc atcaattaat aatacgataa gctcacattc aggatgatta
     4021 tgggtgatgc tttgtgcaat attttgcagt aacacggttt taccagcttt tggcggggca
     4081 acaattaacg ctcgctgacc tttaccgatt ggggcggcta aatctaaaat acgagcggtt
     4141 aaatcttctt ttgatccatt gccacgctcc atttttaagc gagaattggc gtgcagaggg
     4201 gttaagtttt caaagagaat tttactgcga gaggcttccg gtttgtcatc attaactgaa
     4261 tcaactttca gtagggcaaa atagcgttct ccctcttttg gtgggcgaat tttaccttct
     4321 attttatcgc cagtttgtaa gttaaaacgg cggatttggc ttggagaaac ataaatatca
     4381 tcagggccgg ctaaatatga gctatctgat gagcggagga aaccgaagcc gtccggtaga
     4441 atttcaagca cgccttgccc gaaaatgtct tcaccgcttt ttgcgtgttg tttaagaata
     4501 gcaaatacga tgtcttgttt acgtaaacga gccagatttt ctaagcccat ttgattttca
     4561 cccatttcca caagctttga aacaggggtg tttttaagtt gagtaagatg cataatttga
     4621 atgtaaaatt gtgattaatt aagattggat ttgtt
//
LOCUS       DANLXS010000182         4565 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.183, whole genome shotgun sequence.
ACCESSION   DANLXS010000182 DANLXS010000000
VERSION     DANLXS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.183"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(376..1143)
                     /locus_tag="PXD71_002268"
     CDS             complement(376..1143)
                     /locus_tag="PXD71_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL6292340.1"
                     /translation="MSKIIKIGKVANNSDAIDAISTQPRHSVIVGTIKRLQTDRIDLL
                     YQHRVDPNVPIEEVAGAIKDLMKQGKVLNWGLSEAGEKTIRKAHAIQPLTAVQSEYSL
                     FFRGREKDVILVCEELGIGFVPWSPLAMGALAGYVDENSRFEADPSKDLRGIIPRFTP
                     EAMQQNVKLIQLVQKWAKRKLCTPAQLSLAWLMAQKAFIAPIPGTSKARHLLDNLGAD
                     DVKFTEEELAQFRQELEAIEIVGLRLPEAILNFSEAK"
     gene            1142..1462
                     /locus_tag="PXD71_002269"
     CDS             1142..1462
                     /locus_tag="PXD71_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292341.1"
                     /translation="MELKVIHKNLVDSAKIKLHAVYLVIFLFVSHFAMIFGMADPISA
                     GYQPTTPAMTHSMQMDAKQITEMHNQMMTGEITPEEMMKMHQEMNPNMNMPNTQGGHS
                     EHHK"
     gene            1644..2099
                     /locus_tag="PXD71_002270"
     CDS             1644..2099
                     /locus_tag="PXD71_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HDL6292342.1"
                     /translation="MKLHKFTRSLLLLSLGITAFAQAQTLSMEVWKSPTCGCCNEWIS
                     YMQKNGFEVKVNETGNYDAHKRFNIKYEHASCHTAVIDGYVIEGHVPAEDIKRLLAEK
                     PDAIGLSAPGMPIGSPGMDGEAYGGRKDPYDVVLIKKNGESEVFKSYNQ"
     gene            2113..3660
                     /locus_tag="PXD71_002271"
     CDS             2113..3660
                     /locus_tag="PXD71_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HDL6292343.1"
                     /translation="MKRRDFLRYGIGISLASSSLYSLANMMNHAQHGNMAMMHSVPTG
                     LMPTEAMPSGLSLNGILPKLANQSTQAGLFKATLKAEPIKIRLADNKETEFWAYNGQL
                     PGPQIEVFEGDTVEIEFINHLPQPSTVHWHGLDVPNEADGNPMDMVEPQGKKVYRFTL
                     PQGSAGTYWYHPHPHDHVSEQVYKGLAGTFVVKAKNDPLAHLPEQHWVISDLRLNADG
                     TIPANTMLDWINGREGEFVLINGQYQPQIQVKTNERIRLWNATSARYFRLNIPGVKWI
                     VVGTEGGLLEKPRSPVDELLLTPAERVEVIMVGETQGTVNLQSGYYDRRKMMVQEQPK
                     DLTLATIQVKSEPVQLPESLRSLPNWDEPKQVRQIRFSEKMMNHTNMPMMNHGTHHAT
                     TTLTDNPIPPMMNGMFLINGQTFDMNRIDFVTKLNEVEQWEIFNESHMDHPFHLHGTQ
                     FEVIQHTLNGKTFKPEGRALKDVVNLRPYEKVIIRFKQGHTGLKMYHCHILEHENLGM
                     MGMFKVE"
     gene            complement(3750..3905)
                     /locus_tag="PXD71_002272"
     CDS             complement(3750..3905)
                     /locus_tag="PXD71_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL6292344.1"
                     /translation="MGLNRAEMMYREGAYVIDPVFPATLGYAGAGVVVAVGDDAGEFQ
                     IGDKVSV"
     gene            3994..4173
                     /locus_tag="PXD71_002273"
     CDS             3994..4173
                     /locus_tag="PXD71_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292345.1"
                     /translation="MFLVNQLLAITIATSAVLTNAQLAILAVAFWIAILPCARLATAV
                     GVWVFDDVVHNNSPN"
     gene            4222..>4565
                     /locus_tag="PXD71_002274"
     CDS             4222..>4565
                     /locus_tag="PXD71_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292346.1"
                     /translation="MPFKTVTFAQATTNDGQSLLAVNDLFIGPKSHTSAQYILQWNGA
                     EEVQSSSGIIVSTGLGSTGWFQSILAGAMAITGEASHPLLQGFSWSDRKLQFSVREPF
                     PSRTTGVALTFGT"
BASE COUNT         1420 a          932 c          903 g         1310 t
ORIGIN      
        1 tcttttaaat caattaatta aatattaagt ttcccagatt gaagtatgag tacgtaaaaa
       61 taaagtataa aaagactaaa tttcagattt tatttttaca caatgtactt actataaaat
      121 accaaacaga taatatttgt ccataaattc attgagttga ttatgccatt tttacactta
      181 ttgactatag cgttttgcct aatcatctgt ctgattccag ttttaccctt ttcccaatat
      241 ctttcaattt cgataaatct tccactaatc ttgctacata ttcaagaaaa agtgcgatcg
      301 tttttcaaag gattttatga aaacgaccgc tgtaagaaag aatttttatt tacgactttt
      361 tcaggtatct gaaaactatt tcgcttctga aaaattcaaa attgcttcag gcaaacgcaa
      421 gccgacaatt tcaatggctt ctaattcttg gcggaattgc gccaattctt cttcggtaaa
      481 tttcacatcg tctgcgccta aattatccaa caaatgacgg gctttgctcg tcccaggaat
      541 tggcgcgata aacgctttct gtgccattag ccaggccaaa gaaagctgtg caggcgtaca
      601 aagtttgcgt tttgcccatt tttgcacaag ctggattaac ttcacatttt gttgcatcgc
      661 ttcgggtgta aagcgtggga taataccacg caaatctttg cttggatcag cctcaaaacg
      721 gctgttctcg tccacataac cagccaatgc tcccattgcc agcggcgacc aaggcacaaa
      781 cccaatccct aattcttcac aaacgagaat gacatctttt tcacgaccac ggaaaaacaa
      841 cgagtattcg ctttgtacgg cggttaaagg ctgaatagcg tgtgccttgc gaatggtttt
      901 ctcgcctgct tccgacaagc cccaattcaa aactttgcct tgtttcatta aatctttaat
      961 agcgccagcc acctcttcaa tcggcacatt gggatcaaca cggtgttgat agagtaagtc
     1021 aatacggtcg gtttgtaagc gttttatcgt accgactatt actgaatggc gtggttgggt
     1081 agatatcgca tcaattgcat cactattatt agctaccttt cctattttta ttattttact
     1141 catggaatta aaagttattc ataaaaatct cgtggatagt gcaaaaatta aattacatgc
     1201 ggtttatctt gtgattttct tatttgtgag tcactttgct atgatctttg gcatggcaga
     1261 tcctatttca gcaggctatc aacccactac accagcaatg actcattcaa tgcaaatgga
     1321 tgctaaacaa ataacagaaa tgcacaacca aatgatgacg ggcgaaataa cgcctgaaga
     1381 aatgatgaag atgcatcaag aaatgaaccc taatatgaat atgccgaata ctcaaggtgg
     1441 tcattcagaa catcataaat aataattctg atcacatttt aaaaaataat agtggatggt
     1501 acgttggcat atctctacta caagcagtag gattttgaga aaaattttca aatgatagaa
     1561 ttttttcaga ttacttgacc ttaaccctag gttaagcttt aaaatgatgt ccattcaatc
     1621 tcaataaaaa ataaggacat cttatgaaat tacataaatt cacccgctca ttgttgcttt
     1681 taagtttagg cataaccgct tttgctcaag cacaaacact ttctatggaa gtgtggaaaa
     1741 gcccgacttg tggctgttgt aatgaatgga tcagctatat gcagaaaaac ggttttgagg
     1801 ttaaagtcaa cgaaacaggc aattatgatg ctcataagcg ctttaacatt aaatatgaac
     1861 atgcttcttg ccacacagcc gtgattgatg gctatgtgat tgaaggacat gtacctgctg
     1921 aggatatcaa acgtttactc gcagaaaaac ccgatgcaat aggcttgtct gcaccgggaa
     1981 tgccaattgg ttcaccaggt atggacggtg aagcatacgg cggacgcaaa gatccttatg
     2041 atgttgtttt gattaagaaa aacggtgaaa gtgaagtgtt taaatcctat aaccaatagg
     2101 gggggggaaa gaatgaaacg tcgagatttt ttacgttatg gaattggaat tagcttagcc
     2161 agtagctcgc tttatagcct cgcgaatatg atgaaccacg cacaacatgg caatatggcg
     2221 atgatgcatt cagtacctac tggcctaatg ccaactgagg caatgccttc aggtttatcg
     2281 cttaatggga tattaccaaa acttgctaat caatcgactc aagctggact gttcaaagcc
     2341 actttaaaag cagagccaat taaaattcgc ctcgcagaca ataaagaaac ggaattttgg
     2401 gcttataacg gacaactacc agggccacaa attgaagtat ttgaaggaga taccgtagaa
     2461 attgaattta tcaatcacct accacaaccc tcaacagtac attggcacgg cttagatgta
     2521 ccaaatgagg cggatggtaa cccgatggat atggttgaac cgcaagggaa aaaagtctat
     2581 cgctttacgc tacctcaagg cagtgctggg acatattggt accaccctca tccacatgac
     2641 catgtttccg aacaagtcta taaaggctta gcaggcactt tcgttgttaa agcgaaaaat
     2701 gatccgcttg cacaccttcc tgaacaacat tgggtgattt ccgatctgcg tttaaatgcc
     2761 gatggcacca ttccagcaaa cacgatgtta gattggataa acggtcgtga gggtgaattt
     2821 gtgttaatca acggtcaata tcagccccag attcaagtga aaacgaatga acgaatccgt
     2881 ctttggaatg ccactagcgc tcgttatttt cgtttaaaca ttccaggggt gaaatggatt
     2941 gtggtgggta ccgaaggtgg tttacttgaa aaacctcgct cgcctgttga tgaactgcta
     3001 cttacaccag ccgaacgcgt tgaagtgatt atggtgggtg aaacgcaagg aacagtcaat
     3061 ttacaaagcg gttattatga tcgccgtaag atgatggttc aagaacagcc taaagatctc
     3121 actttagcca caattcaggt gaaatcagaa cctgttcagt tacctgaaag cttaaggtcc
     3181 ttgcctaact gggatgagcc aaagcaggtt cgccaaattc gctttagtga aaaaatgatg
     3241 aaccatacga atatgccaat gatgaatcac ggtacacatc acgctactac aacactaacg
     3301 gacaacccta ttccaccaat gatgaatggt atgttcttaa tcaacggtca aacctttgat
     3361 atgaaccgta ttgattttgt taccaaattg aatgaagtgg aacagtggga aatcttcaat
     3421 gaaagccata tggatcatcc attccattta cacggcactc aatttgaagt aattcaacat
     3481 acgttaaacg gtaaaacctt taagccagaa ggcagagcgt taaaagatgt ggttaattta
     3541 cgcccttatg aaaaagtgat tattcgcttt aagcaaggac atactgggtt gaaaatgtac
     3601 cattgccata ttttagaaca tgaaaatctc ggtatgatgg ggatgtttaa agtggaatag
     3661 tggtatctaa ctataaaaat agagggattt cgtcataaaa aatctgcacc ttaatcagtt
     3721 ggtttttagt ccaacttttg gggtgcagat cagacgctca ctttgtcgcc gatttgaaat
     3781 tcgccagcgt catcgcccac tgccacgacc acgcctgccc ctgcataacc caaagtcgca
     3841 gggaacacag ggtcaatcac atacgcccct tcccgataca tcatctcggc acggtttagt
     3901 cccaatgcac ggcacggttt ttaaacattt tcattatagt tttccttatc aaaatgggat
     3961 ttggcaaaca ttgggcaaac ctaagcccgc ccaatgtttt tagttaatca attacttgca
     4021 atcacaatcg ccacaagtgc agttttgaca aatgcacagc tcgccatttt ggcagttgca
     4081 ttttggattg caattttgcc ctgtgcaaga ttggcaaccg cagtgggcgt ttgggttttt
     4141 gatgatgtcg ttcataacaa ttctccaaat taaaggttga ttatcaaaac aaaaaccgtt
     4201 tttaaaccca ttaataaaaa aatgccattt aaaacggtca cttttgcaca agcaacaacc
     4261 aatgatggtc aatccttatt agcggttaat gacttattta ttggccctaa aagccacact
     4321 tccgcacagt atattttgca atggaatggc gctgaagaag tgcaatcttc atcaggcatt
     4381 attgtatcaa caggattggg atcaacgggg tggtttcaat ctattcttgc tggtgcgatg
     4441 gcaattacag gagaagcttc gcaccctcta ttacaaggct ttagctggag tgatcgaaag
     4501 ctacaattta gtgtaagaga gccatttcca agtagaacaa caggtgttgc actgactttt
     4561 ggcac
//
LOCUS       DANLXS010000183         4458 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.184, whole genome shotgun sequence.
ACCESSION   DANLXS010000183 DANLXS010000000
VERSION     DANLXS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.184"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            121..4017
                     /gene="purL"
                     /locus_tag="PXD71_002275"
                     /gene_synonym="purI"
     CDS             121..4017
                     /gene="purL"
                     /locus_tag="PXD71_002275"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDL6292347.1"
                     /translation="MTIQTFRGSPALSAFRIQQLLQKFQQNQLPVKSVYAEFLHFVAL
                     NRPLVADEENKIKELLHYGPTLEEDEPTGFCLIVTPRIGTISSWSSKATDIAHNCGLA
                     AVERIERGTAYYLEFSETPNESQLTTLKGLLHDRMMETVLDNESQAEQLFAQQEPKPF
                     TSVDILGGGRKALEEANINLGLALADDEIDYLVENFTQLGRNPNDIELYMFAQANSEH
                     CRHKIFNADWTIDGVKQEKSLFKMIKNTFEKTPDYVLSAYKDNAAVMEGSTVGRFFPD
                     QDGQYRYHQEDAHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLT
                     GFSVSNLVIPKFEQPWENPLSKPNRIASALDIMIDGPLGGAAFNNEFGRPALLGYFRT
                     YEEKVNSFAGEEIRGYHKPIMLAGGIGNIRAEHVQKGEIPVGAKLIVLGGPAMNIGLG
                     GGAASSMASGKSKEDLDFASVQRENPEMERRCQEVIDRCWQMGAENPILFIHDVGAGG
                     LSNAMPELVHDGECGGKFDLRNILCDEKGMSPLEIWCNESQERYVLAVSPEKLDIFTA
                     LCERERAPFAVIGEATKEKHLTLADPHFDNKPIDLPMNVLLGKTPKMQRDVLSKTVKN
                     PPLATADIDLKEAFHRVLRLPAVAEKTFLITIGDRSVTGMVAQDQMVGPWQIPVADCA
                     VTTASLDSYHGEAMSIGERSPVALLDFAASARLAVAESITNIAATNIGDIKRIKLSAN
                     WMSAAGHEGEDAGLYEAVKAVGEELCPELGITIPVGKDSMSMKTTWEENGEQKSVTAP
                     LSLVISAFARVEDVRKTVTPQLRTDKGDTRLLLIDLGEGNNRLGATALAQVYKQLGDK
                     PADVVNVARLKGFFNAMQTLVAQGKLLAYHDRSDGGLITTLAEMAFAGNCGISVEIDR
                     LVDDLAVLFNEELGAVIQVRTEELSEVQAVLNAENLANLTFDIGTAVESDSFEITRNG
                     TTVFSQKRSELRGIWAELTHQMQRLRDNPECADQEFAAKKDPNNKGLSAFLTYDVNED
                     IAAPYINKGVKPSVAILREQGVNSHYEMAAVFDRAGFNAIDVHMSDLISGRRNLNDFN
                     AMVACGGFSYGDVLGAGGGWAKSILFNPMLRDQFSKFFANPNTLALGVCNGCQMVSNL
                     AEIIPGTENWPRFVRNKSERFEARAAMVKINDTNSLWFNGMAGSHMPIAVSHGEGQVE
                     FKNPQQLANLQQQNLVIAQYVDSHLNPTEQYPANPNGSALGITAIGNTDGRVAIMMPH
                     PERVFRAVSNSWCPEDWTEGGAWMRLFRNARVALKY"
     gene            4372..4458
                     /locus_tag="PXD71_002276"
                     /pseudo
     CDS             4372..4458
                     /locus_tag="PXD71_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1241 a         1021 c         1107 g         1089 t
ORIGIN      
        1 atttatttga tactaatgaa taaatgtatc taacagcaaa cgtctgacaa atatagcaat
       61 cgtttgcgca atcgtttata atcggtttag tttttcatct taaataggaa aatccaaaga
      121 atgacaatcc aaaccttccg tggctcgcct gccttatctg cttttcgcat tcaacaactt
      181 ctgcaaaaat tccaacaaaa tcaattacct gtaaaatcag tttatgcgga gtttttgcat
      241 tttgtggcat taaatcgacc gcttgtagcc gatgaagaaa acaaaatcaa agagttattg
      301 cactacggtc caacgctgga agaagacgag ccgacagggt tttgcctgat tgttacccct
      361 cgtatcggca cgatttcctc gtggtcgtct aaggcaaccg acatcgccca taactgcggt
      421 ttggcagcag tagagcgaat tgagcgtggc acggcatatt atttagaatt ttctgaaacg
      481 ccgaacgaaa gtcaacttac gacgttgaaa ggcttattac acgaccgtat gatggaaacg
      541 gtgctggata acgaaagcca agccgaacag cttttcgctc agcaagagcc gaagccgttt
      601 acttcggtgg atattttagg cggtggtcgc aaggcactgg aagaagcgaa tatcaactta
      661 ggtttagcgt tggcggacga tgaaattgat tacttggtgg aaaactttac ccaacttggg
      721 cgtaacccca atgatattga actctatatg ttcgcccaag ccaactcgga gcattgccgc
      781 cacaaaattt ttaatgcgga ttggacgatt gatggggtaa aacaggaaaa atccctgttt
      841 aagatgatta aaaacacctt tgagaaaacc ccggactatg tgctttccgc ctacaaagac
      901 aatgcggcgg tgatggaggg ctcaacggtt gggcgtttct tccccgacca agatggacaa
      961 tatcgctatc atcaagaaga tgctcatatt ttaatgaaag tagaaactca caaccaccca
     1021 accgccatct cgccattccc gggagcggcg accggttcgg gcggtgaaat tcgtgatgaa
     1081 ggggcgacag ggcgtggggc gaaaccgaaa gcgggcttga cgggtttttc ggtctctaac
     1141 ttagtgatcc caaaatttga acagccgtgg gaaaacccgc tttccaagcc gaatcgcatt
     1201 gcctccgcac tggatattat gattgatggc ccactaggcg gagcggcatt taacaatgaa
     1261 tttggtcgtc ctgcgttatt gggctacttc cgtacttacg aagaaaaagt gaacagcttc
     1321 gcaggcgaag aaattcgtgg ctatcacaag ccgattatgt tagcaggcgg tatcggcaac
     1381 attcgtgccg aacacgttca aaaaggcgaa atcccggtgg gagcgaagtt gatcgtgctt
     1441 ggtggcccgg caatgaacat cggcttaggc ggtggggcgg catcgtcaat ggcatcgggg
     1501 aaatcgaaag aagatttgga ttttgcctcg gttcagcggg aaaaccctga aatggaacgc
     1561 cgttgccaag aggtgatcga ccgttgctgg cagatggggg cggagaaccc aattttattt
     1621 attcacgacg tgggggctgg cggtttatca aatgcgatgc ctgaattggt tcacgacggc
     1681 gaatgtggcg gtaagtttga tttgcgtaac atcttgtgcg atgaaaaagg aatgtcgcca
     1741 cttgaaattt ggtgtaacga atcgcaagag cgttatgtgc ttgccgtttc ccctgaaaaa
     1801 ctcgacattt tcacagcatt gtgcgaaagg gaaagagccc catttgcggt gattggcgag
     1861 gcaacgaaag agaagcattt aacccttgct gaccctcatt ttgacaataa accgattgat
     1921 ttgccgatga atgtgttgct cggcaaaacg ccaaaaatgc agcgagatgt tttgtcaaaa
     1981 actgtgaaaa atccaccgct tgcaaccgct gacatcgact tgaaagaggc gttccaccgt
     2041 gtgctacgct tgcctgcggt ggctgaaaaa accttcctga ttaccatcgg cgatcgctca
     2101 gtaacgggaa tggtggcaca agatcagatg gtcggcccgt ggcaaattcc tgtggcagat
     2161 tgtgccgtaa ccaccgcttc attagacagc tatcacggtg aagcaatgtc aattggcgag
     2221 cgatcacctg ttgctttgct tgactttgct gcctcagctc gcctcgccgt ggcagaaagc
     2281 atcaccaata ttgccgccac taacattggc gacatcaaac gcattaaact ctcggcgaat
     2341 tggatgtcgg cggcagggca cgagggcgaa gacgcagggc tttacgaggc agtaaaagcg
     2401 gtgggcgaag agctttgccc tgaattaggc ataaccattc cggtaggcaa agactcaatg
     2461 tcgatgaaaa ccacttggga ggaaaacggc gaacagaaat ctgttactgc tccgctttcg
     2521 ctcgtgattt ccgctttcgc tcgtgtggaa gatgtgcgta aaaccgttac cccacaactt
     2581 cgcaccgaca aaggcgatac ccgcttgttg ctgatcgacc tgggcgaagg caacaaccgc
     2641 ttgggtgcaa ccgcacttgc tcaagtttac aaacaacttg gcgacaaacc tgccgatgtg
     2701 gtgaatgtgg ctcgcttaaa aggcttcttc aacgcaatgc aaacccttgt cgcacaagga
     2761 aaattgcttg cctaccacga ccgctcggac ggtggtttaa tcaccacact ggcggaaatg
     2821 gcgtttgcag ggaattgcgg aatttcggtg gaaatcgacc gcttggtgga cgatttagcc
     2881 gtgttattca acgaagaact gggggcagta atccaagttc gcaccgaaga attgagcgag
     2941 gtgcaagcgg tgttaaatgc cgaaaatctt gcaaatctca cctttgatat tggtacagcg
     3001 gttgaaagcg acagctttga aattacccgc aacggcacaa cagtatttag ccaaaaacgt
     3061 tccgaactgc gtggcatttg ggcggaactc actcatcaaa tgcagcgttt gcgagacaac
     3121 cctgaatgtg cagaccaaga atttgcagcg aaaaaagacc cgaataacaa aggcttatcc
     3181 gcatttttaa cttacgatgt gaacgaagac atcgccgccc catacatcaa caaaggcgta
     3241 aaaccgagtg tggcgatttt gcgtgagcaa ggtgtaaaca gccactacga aatggcagcc
     3301 gtctttgacc gtgccggttt caacgccatt gacgtgcata tgtcagactt gatttcaggc
     3361 agacgcaacc tcaacgactt caacgcaatg gtggcgtgcg gaggcttctc ttacggcgat
     3421 gtcttaggtg caggcggcgg ctgggcgaaa tcgattctgt tcaacccaat gctgcgtgac
     3481 caattcagca aattcttcgc caacccgaac acgctggcat taggggtatg taatggttgc
     3541 caaatggtct ccaatctagc ggaaattatt ccaggcacag aaaactggcc acgcttcgtg
     3601 cgtaataaat ccgaacgttt tgaagcccgt gctgcaatgg tgaaaatcaa cgacaccaac
     3661 tcactttggt tcaacggaat ggcaggcagc catatgccga ttgcggtttc acacggtgaa
     3721 ggacaagttg agttcaaaaa tccacagcaa ttagcgaact tgcaacagca aaatctcgtg
     3781 atcgcacaat atgtggatag ccacctcaac ccaaccgaac aatacccagc caacccgaac
     3841 ggatcggcgt tgggtatcac cgctatcggt aatacagacg gacgagtcgc tattatgatg
     3901 ccacaccctg aacgtgtgtt ccgtgcggtc agtaactctt ggtgcccaga agattggacg
     3961 gaaggtggag cgtggatgcg gttgtttaga aatgcgagag tggcgttgaa gtattaaaaa
     4021 tctaccttcg tagtgatctg cccccatagg gggcgattaa aattcgaatt catagaaccc
     4081 gaatttttat atatctgata aacgaagttg tttatccttc gtgcttttcc tgatatttca
     4141 agtactttct tatcatttct gcattgacat ctacggtgtc tacgcagtag cctcttgcca
     4201 caaaagtggg ttgccccaca atttatgctt acgtaaatag ggaaacttat tgaaaaatct
     4261 tattggaccc tgttcaccct tttaaatgcc ccattacgtc ttatacagaa aatgaacgtg
     4321 atttatttga acggtcaatt ctaccttaat agaaagtggt ttttagttag caatcaaaat
     4381 cacattaacg gaattgagaa tttggagagt caggcaaaat gctatttgcg taaatttaac
     4441 ggcgttccta aagtacat
//
LOCUS       DANLXS010000184         4453 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.185, whole genome shotgun sequence.
ACCESSION   DANLXS010000184 DANLXS010000000
VERSION     DANLXS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.185"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..155)
                     /locus_tag="PXD71_002277"
     CDS             complement(<1..155)
                     /locus_tag="PXD71_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011497995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6292348.1"
                     /translation="MFYSNNPLIKHKSGLLNLAEELGNISQACKVMGMSRDTFYRYQQ
                     AVEQGGV"
     gene            complement(239..778)
                     /locus_tag="PXD71_002278"
     CDS             complement(239..778)
                     /locus_tag="PXD71_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6292349.1"
                     /translation="MLDSLKEVNQKLDKKADKVGGGTKEDALVTLDTLIASGTVKAET
                     AMSLLPTLQKGAVATGASSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGSFEL
                     AYMASWLPQQMAAAKQAGLSSIEGFERLLIANQQARVTAGTSDEAGNNLVNLLGKITA
                     KETNERFKNIEYKSVEDQT"
     gene            877..1128
                     /locus_tag="PXD71_002279"
     CDS             877..1128
                     /locus_tag="PXD71_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6292350.1"
                     /translation="MAPTISDGDQMLVDFSKKEARGDDKIYLVQNGESVWVKRVRKVW
                     DYVELISDNESYRPIRITADEAQNLQIIGQVVHIGHSLV"
     gene            complement(1324..1524)
                     /locus_tag="PXD71_002280"
     CDS             complement(1324..1524)
                     /locus_tag="PXD71_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL6292351.1"
                     /translation="MQYVLDLTGYACPLPLLMAKKAMNDLKKGDSLEILLNQQSSLTD
                     FELLAKEQGWLCELQAVENVGY"
     gene            complement(1583..2095)
                     /locus_tag="PXD71_002281"
     CDS             complement(1583..2095)
                     /locus_tag="PXD71_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HDL6292352.1"
                     /translation="MQQLEPQTFASWAEPIDMLYACHSKVKSFCKQLQILPDYLEKNG
                     VNQAVKNDVQQILNYFNISAPLHHEDEEDDFFPELIKVQPQAQAEVDELERQHVHLHK
                     NWDYLSAQLEDLLAGKRENVDRDLIERFVAGYDVHIAIEEPLFELGREHLAQDKLSAM
                     GKVMADRRKL"
     gene            complement(2108..3262)
                     /gene="tgt"
                     /locus_tag="PXD71_002282"
     CDS             complement(2108..3262)
                     /gene="tgt"
                     /locus_tag="PXD71_002282"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL6292353.1"
                     /translation="MKYELKTTSGNARRGRLTFNRPKGEFHVETPAFMPVGTYGTVKG
                     MTPEEVKATGAQILLGNTFHLWLRPGQEVMKMHGDLHDFMQWHGPILTDSGGFQVFSL
                     GKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
                     KSMEMSLRWAKRSRDRFDELENPNALFGIIQGGIYEELRKVSVEKLVEIGFDGYAVGG
                     LAVGEPKEDMHRILEYVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNA
                     RNGHLFVTNGIVKIRNAKYRADTTPLDPECDCYTCKNYTKAYLYHLDKCGEILGARLN
                     TIHNLRYYQRLMAEIRQAIEEDRFDDFVVEFYARIGKEVPPLQSEVKEQK"
     gene            complement(3321..3461)
                     /locus_tag="PXD71_002283"
     CDS             complement(3321..3461)
                     /locus_tag="PXD71_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292354.1"
                     /translation="MKKLLLCVGILVLAACSSTGVGGSIGAGGGSNGVGIGLGVGTDF
                     RF"
     gene            3555..4127
                     /locus_tag="PXD71_002284"
     CDS             3555..4127
                     /locus_tag="PXD71_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HDL6292355.1"
                     /translation="MKILRKFAAVCVVSMAMLSANANLLTISEQEINDYLATKLAEKI
                     PLKDKVGIPKLFELDYRLHSLTSQIGRTEEKKVAISGVVDGLLNAKGKKYAASIHLNM
                     DTTPYYDPQKGALFLKDVRLLSWSATPAKYQAELQMFLPVLADGLSSFLNSTPVYTLD
                     ENKTKEAMVKKFGKAIVVENGNIKLETTIF"
     gene            4229..4447
                     /gene="cspD"
                     /locus_tag="PXD71_002285"
     CDS             4229..4447
                     /gene="cspD"
                     /locus_tag="PXD71_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HDL6292356.1"
                     /translation="MDIGIVKWFNNVKGFGFITSDVCEGDIFAHFSEIQAEGYRSLKV
                     GQKVQFELVQGERGASASNITPISEKTL"
BASE COUNT         1291 a          969 c          831 g         1362 t
ORIGIN      
        1 tcaacaccac cttgctcaac cgcttgttga taacgataga atgtatctcg gctcattccc
       61 attactttgc aggcttgaga aatattaccc agttcttctg ctaaatttaa taaaccgctc
      121 ttgtgtttaa tgagagggtt gttagaataa aacatgagag tttccttttt gtttagattg
      181 atttttgaca ctcatattct aaacgggaaa ctctcacttt ttagagtgaa ttgtcagatc
      241 aagtctgatc ttctacactt ttatattcaa tatttttaaa acgctcattt gtttctttag
      301 ctgtaatctt accaagcaga tttaccagat tattacctgc ctcatcgctt gtaccggcag
      361 tgacacgagc ctgttggttt gcaattaaaa gtcgctcaaa gccctcaata ctactcaaac
      421 cggcttgttt tgcagctgcc atttgttgag gcaaccaact tgccatatat gcaagctcaa
      481 aactacctgc ttgcccggct gcaacagcca tatccaatgc ccgcccaata tcctcttctt
      541 tgatgccaaa ttgttgcata gaggagatcg caatttttgc catatcctcc gagcttgccc
      601 ctgttgccac cgcccctttc tgcaaagtag gtaatagact cattgcggtt tcggctttga
      661 ctgtaccgga ggcaatgagc gtatcaagcg taactaacgc atcctctttt gtgccacccc
      721 caaccttatc cgccttttta tcaagttttt ggtttacttc tttgagcgag tctaaaatat
      781 cgtccagctt ttccttaaga gccttattca gacagcctat taagcctagg cgtaaaagcc
      841 cataattgtg gcgtattttg ggctaatggc acatcaatgg ccccgactat cagcgatggt
      901 gatcaaatgt tagtggattt cagcaaaaaa gaagcccgtg gagacgataa gatttatctt
      961 gtgcaaaatg gtgaaagcgt ttgggtaaaa cgtgtaagaa aagtgtggga ctacgttgaa
     1021 ttgattagcg ataatgagtc ctaccgccca atccgaatta cagcagacga agctcaaaac
     1081 ttacaaatca tcgggcaggt agtccacatc ggacactctt tagtttaaaa cagatttaaa
     1141 atagttttaa aaccgatcaa acctatgcaa aaaatatcgc aaaaactgcc catttttttc
     1201 taatcctgct cattttgttt tttgcatagc cccaaccaaa caataacggc gaacccttgt
     1261 attcacgggg ctcgccgtta tttttccgcc caaatttttt tctttctctc tatgcaaaaa
     1321 agatcagtac cccacatttt caaccgcttg tagctcacaa agccaacctt gctctttggc
     1381 aagcagttca aaatcggtaa ggctactctg ctgattgagt aaaatttcca agctatcgcc
     1441 ctttttaaga tcgttcatcg ctttttttgc cattaataac ggcaaggggc aggcatagcc
     1501 ggttaaatct aaaacatatt gcatagcgaa atagggaata aagaaaaagg gcgaatattg
     1561 aaattcgccc aatcacaaaa gattagagtt tacggcggtc tgccatcact ttgcccatag
     1621 ccgataattt gtcttgagct aaatgctctc gcccaagctc aaagagtggc tcttcaatcg
     1681 caatatgcac atcataacct gccacaaaac gctcaattaa gtctctatcc acattttcac
     1741 gtttacctgc gagcagatct tcaagctgtg ccgacaaata gtcccaattt ttatgtaaat
     1801 gaacgtgctg gcgttccagt tcgtctacct ctgcttgagc ttgtggttgc accttaataa
     1861 gctcagggaa gaaatcatct tcttcatctt cgtgatgtaa aggggcagaa atgttgaagt
     1921 agttcaaaat ctgctgcacg tcattcttca ctgcttggtt taccccgttt ttctcaaggt
     1981 aatccggcaa aatttgcaac tgtttacaga aactttttac tttgctgtgg caagcataaa
     2041 gcatatcaat cggctctgcc cagcttgcaa aggtttgtgg ttcaagttgt tgcatataca
     2101 ctccttgtta tttctgttct tttacttcag attgtaaagg tggcacttct ttgccaattc
     2161 gggcataaaa ttccactaca aaatcatcaa aacgatcttc ttcgatggcc tggcgaattt
     2221 ctgccattag gcgttgatag tagcgtaaat tatgaatggt gtttagtctt gcccctaaaa
     2281 tctcgccaca tttgtctaag tgatataaat aggctttggt gtagtttttg caagtgtaac
     2341 aatcacattc aggatcaagc ggtgtggtgt ctgcacgata tttcgcatta cgaattttta
     2401 caatgccgtt ggtcacaaat aaatgtccgt tacgggcatt gcgggttggc attacgcagt
     2461 caaacatatc aataccacgg cgaacccctt cgactaaatc ttctggcttg cctacaccca
     2521 ttaaatagcg tggtttatct gccggaattt gcgggcaaac gtattctaaa atgcggtgca
     2581 tatcttcttt aggttcacct accgccaagc caccgactgc atagccatca aagccgattt
     2641 caactaattt ttccaccgag actttgcgaa gttcttcata aataccacct tgaataatgc
     2701 caaataacgc attcgggttt tcaagttcat caaagcgatc acgagaacgt ttcgcccaac
     2761 gtagtgacat ttccatcgat ttttttgcat aatcaaaggt tgccggataa ggagtacatt
     2821 catcaaaaat cattacaatg tctgatccaa gatcgtattg gatctccatt gatttttccg
     2881 gcgaaaggaa aattttctca ccgctaatcg ggttttggaa agttacgcct tcttctttaa
     2941 ttttacgcag tttacctaag ctaaaaactt ggaaaccgcc ggagtcggtt aaaatagggc
     3001 cgtgccattg cataaaatcg tgcaagtcac cgtgcatctt catcacttct tgccccggtc
     3061 ttaaccataa atggaaagtg ttgcctaata aaatttgcgc ccctgtcgct ttaacttctt
     3121 caggagtcat tccttttacc gtgccataag tgccaacagg cataaatgcc ggtgtttcaa
     3181 cgtgaaattc gcctttcggg cggttaaaag ttaaacgccc acgacgagcg ttaccacttg
     3241 ttgtttttaa ttcgtacttc attttaatcc acaaataaac agttcgtgaa agcggtcaaa
     3301 aaccaccaaa aatttaccaa ttagaatcga aagtcggtgc cgacccctaa gccgattcca
     3361 acgccgttag agccaccacc ggctccaata ctgccaccca caccggttga tgagcaagca
     3421 gctaacacta aaatgccaac gcataataag agttttttca ttttttgctg tcctaaacga
     3481 aaaagagagt ctatgataga cccaatgtat tctaacctgt ttaatggatt aaacctaatt
     3541 ttaaagggta aattatgaaa attttacgta aattcgctgc tgtatgcgtt gtctcaatgg
     3601 ctatgctctc tgctaacgct aatttgctca ctattagcga gcaggaaatc aacgactatt
     3661 tagccaccaa attagctgaa aaaattcctt taaaagataa agtcggtatt cctaaattat
     3721 ttgaattgga ttatcgcctg cattcactta ccagccaaat tggcagaacc gaggaaaaaa
     3781 aagtagctat ttctggcgtt gttgatggac ttttaaacgc aaaaggcaag aaatatgctg
     3841 cctctatcca tttaaatatg gataccacgc cttattatga tccacaaaaa ggggcattat
     3901 ttcttaaaga tgtcagattg ttaagttggt ctgcaacacc ggctaaatat caagcagagc
     3961 tgcaaatgtt tttgccggtg ttggctgatg gattaagtag ttttttaaac agtacgcctg
     4021 tttatacttt agatgaaaat aaaacaaaag aagcaatggt gaaaaagttc ggcaaagcca
     4081 ttgtagtaga aaatggtaat atcaagctag aaaccactat tttttaaata attttgtgat
     4141 cttcgtctaa tttttacact ttgatagttg agcttttacg cgttttattt acaatccaaa
     4201 tatcgccatt cattatttgg agagttctat ggatatcggt attgttaaat ggtttaacaa
     4261 tgtaaaagga tttggtttta ttacatcgga tgtatgtgaa ggagacattt ttgctcattt
     4321 ttctgaaatt caagcagaag gttatcgctc tttaaaagtc ggccaaaaag tacagtttga
     4381 attagttcaa ggagagcgag gtgcctcagc ctcaaatatc acgcctataa gcgaaaaaac
     4441 gttataatcc gtc
//
LOCUS       DANLXS010000185         4401 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.186, whole genome shotgun sequence.
ACCESSION   DANLXS010000185 DANLXS010000000
VERSION     DANLXS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.186"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..2027
                     /locus_tag="PXD71_002286"
     CDS             <1..2027
                     /locus_tag="PXD71_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDL6292357.1"
                     /translation="HCEIRRRPLVGKTFIAQKRPNQIIGWGLRPSAVGSRAYAQKHQL
                     PYIALEDGFLRSLGLGVKGYPPFSLVYDDIGIYYDTTCPSRLEKLLLEKPLQDQEQIK
                     LAERALSQILTYGLSKYNHALDFSEIPSAKEKIVLVIDQTFGDMAVKYGQASEVDFNK
                     MLETAISENPEAQIWVKTHPDVLSGKKKGYLTDLPQYTDRVRIITEDVNPASLIKLCE
                     KVYCVTSQMGFEALLLGKKVVTFGVPWFAGWGVTDDRNEAVQLLVQQNRRKDRSLLQL
                     FYAAYFDYSRYINPNTGEKGSIFDVIDYLYKAKRLNQRLSGNLYCIGMSLWKQAVIKP
                     FFNLPQCRLHFVRSLNSLKKRPLADNAKILVWSNGKADVLQFAQQNNIPLLRMEDGFI
                     RSVGLGANLVAPLSLVVDDLGIYFNAENTSRLEQILLSAEHFSETDLSYAETLRQKLV
                     ASHIGKYNVGGGDVKFNAQGKKIILVPGQVEDDASIKSGSPQIKRNLDLLKKVRELNP
                     SAYIIYKPHPDVVSGNRVGQIPTAETRRFADQMVDNANILDCILIADEVHTMTSLAGF
                     EALLRGKKVHCYGLPFYAGWGLTEDRLKSARRGKSLTLSQLIAATLIYYPLYVDPISR
                     QFIDANQAIHILSQQKRNLTNQRIKRSWVSKQYGKLQHLYRAFKYASHTK"
     gene            2050..3282
                     /locus_tag="PXD71_002287"
     CDS             2050..3282
                     /locus_tag="PXD71_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL6292358.1"
                     /translation="MEHYLDELIINSQRILLLQGPIGSFFTEFARWLESEQKEVFKLN
                     FNGGDEYFYPNNSPNTFPYYDSLDNFEAYLIQFCQTHQIDNILCFGDNRYYHKVAKNV
                     CLKLKLVFWAFEEGYFRPEYITLEKWGVNAFSPIPKNHTFFLPYAELPVVSEPEKVSK
                     GFRCMAGKAIPYYWNAYKKRKAYAKYQHHRFLNLKYYLNLWTRSGIKRICYWLHDKGF
                     AKRVEKGEFGEFFIVPLQVYDDTQVRIHSEQKSVERFLREVLNSFAKFAPAHLNLIIK
                     HHPMDRGFIDYKMVIYEFLEQYPQLNGRVFYIHDVPLPVFLRYGKGMVTLNSTSGLSA
                     LLHNMPVKTLGRANYDFEGLTDKQPLDTFWANPQPPNSEVFKGYRQFHLHKTHINGNF
                     YHKSILRYPYNWENEAHR"
     gene            3363..>4401
                     /locus_tag="PXD71_002288"
     CDS             3363..>4401
                     /locus_tag="PXD71_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6292359.1"
                     /translation="MAIYAIWNNKGGVGKSYLTFQIACEYAKQYPAKKVLVVDLCPQA
                     NSSSMLLGGIEKGEQELAKIHSQVNKNTISGYISERLVSPYRSINSATKYVTQVSTIN
                     EYVPKNVYLVVGDEELEIQSSGILHASQAVHVPDSWRLVHLWVRDLVSDIQNAWDNDD
                     NVVFIDCNPSFTIYTELALAASDRLIIPFSADGSSKRAVKSVLSLVYGVKRHSGDVSS
                     HFYTESQKSKLALPQIYMYVGNRLTINAKASATAFKTVVEEIGDEIYNIWKTNANLFC
                     IHPAGVGTPTNKSSFRKMFQYEVRDANTASVVSGALGIPISRLSSGKRDVLGKNVMVN
                     QSQLDAQVPNIQ"
BASE COUNT         1416 a          698 c          898 g         1389 t
ORIGIN      
        1 ctcattgtga aattaggcga agacctcttg tgggaaaaac ctttattgct caaaaaagac
       61 cgaaccaaat catcggctgg gggcttcgtc cttcagcggt gggatcaaga gcttatgcac
      121 aaaaacatca acttccctat attgcattag aagacggctt tttacgctca ctagggctgg
      181 gtgtaaaagg ctatccgcca ttttctttag tttatgatga tataggtatt tattacgata
      241 ccacctgtcc ttctcgttta gaaaagttat tgcttgaaaa acctttacag gatcaagagc
      301 aaatcaagct tgcagagaga gcattatctc aaatcttaac ttacggactt tctaaatata
      361 atcacgcact agattttagt gaaatcccct ctgccaaaga aaaaatcgtg ttagttattg
      421 accaaacctt tggtgatatg gcggtgaaat atggtcaagc gagtgaagtg gatttcaata
      481 aaatgttgga aacggcaatc agtgagaatc cagaggctca aatttgggta aaaactcacc
      541 ccgatgtatt aagtggcaag aagaaaggct atttgaccga tttacctcaa tatactgata
      601 gggtcagaat tattactgaa gatgtgaatc cggcaagttt gattaaattg tgtgagaaag
      661 tctattgcgt aacttcgcaa atggggtttg aggcattatt attaggtaaa aaagtggtta
      721 cctttggtgt accgtggttt gccggttggg gggtaacaga tgatcgtaac gaagcggttc
      781 aactattagt tcaacaaaat agacggaaag atcgttcatt actgcaactt ttttatgctg
      841 cgtattttga ttacagccgt tatattaatc cgaataccgg tgagaaaggc tctatttttg
      901 atgtcatcga ttatctatat aaagccaaaa gattaaatca gcgcttaagt gggaatctat
      961 actgtattgg tatgtcgcta tggaaacagg cggtaattaa gccgtttttt aatttgccac
     1021 aatgtcgatt acattttgtt cgttcgctaa attctttaaa aaaacgaccg cttgcagata
     1081 atgcaaaaat tttagtttgg agcaatggca aagcggatgt cttgcaattt gcacagcaga
     1141 ataatattcc attgttaaga atggaagatg gattcattcg ctctgtcgga ctaggcgcta
     1201 atttagttgc accgctttct ttagtagtcg atgatttagg catttatttt aatgcagaga
     1261 acacttcacg attagaacaa attttattga gtgcagagca tttttctgaa actgatttgt
     1321 cttatgctga aacattaagg cagaaattgg tggctagcca tattggaaaa tataatgtgg
     1381 gcggtggcga tgtaaaattt aatgctcaag gtaaaaaaat aattcttgta ccggggcagg
     1441 tagaagatga cgcttcgatc aaatcaggtt ctccacaaat taaaagaaat ctggatttac
     1501 tgaaaaaagt gagggaatta aaccctagtg cctatattat ttataagcct caccctgatg
     1561 tggtaagtgg taatcgagtc ggtcagattc ctacggcaga aacacgcagg tttgcggatc
     1621 agatggtgga taatgccaat attttagatt gtattttaat tgcagatgaa gtgcatacaa
     1681 tgacatcttt agccggtttc gaagcattat taagaggtaa aaaagtgcat tgctacggat
     1741 taccttttta tgctggttgg ggattgacgg aagatagatt gaagtcggca cgcagaggta
     1801 aatctttgac attatctcag ttaattgccg caacattgat atattaccct ctgtatgttg
     1861 atcctataag caggcagttt attgatgcca atcaagcgat tcatatattg tcacaacaga
     1921 aacgaaacct aaccaatcaa aggattaaac gttcttgggt gagtaaacaa tatggaaaat
     1981 tacagcatct ttatcgtgcg tttaaatatg cttcccacac taaataagcg tagagggtag
     2041 gaaattaaga tggaacatta tttagatgag ctaatcatca atagccagag aattttattg
     2101 ttacaaggcc cgataggctc ttttttcact gagtttgccc gttggttaga aagtgagcaa
     2161 aaagaggtct tcaagttaaa ttttaatggc ggagatgaat atttctatcc aaataatagc
     2221 ccgaatactt ttccttacta tgatagctta gataattttg aagcctattt aattcagttt
     2281 tgccaaactc atcaaataga taatatctta tgttttggcg ataatcgtta ttatcataaa
     2341 gtggctaaaa acgtttgctt gaagttaaag ttggtctttt gggcgtttga agaagggtac
     2401 tttaggccag aatatattac gttggaaaaa tggggtgtaa atgctttttc tccaatccct
     2461 aaaaatcaca ctttcttttt gccttatgcc gagttaccag tagtcagtga accagaaaaa
     2521 gtatctaaag gctttagatg tatggcggga aaagcaatcc cgtactattg gaatgcctat
     2581 aaaaaacgta aagcatacgc aaaatatcag catcatcgtt ttctgaatct caaatactac
     2641 cttaatttat ggacaaggtc gggaattaag cgaatatgtt attggttaca tgataaaggc
     2701 tttgccaagc gggtagaaaa aggagagttt ggtgaatttt tcattgtgcc tctacaagtt
     2761 tatgatgaca ctcaagtgag gattcatagt gaacaaaaaa gtgtagagcg ttttttaagg
     2821 gaagttctga attcctttgc caaatttgca ccggcacatc tcaatttaat tattaagcat
     2881 cacccgatgg atagaggatt tatagattat aaaatggtga tttatgaatt cttagagcaa
     2941 tatccccaat taaacggtag agttttttat attcacgatg tgccgttacc tgtcttttta
     3001 cgttatggga aaggaatggt gactttaaat tcaaccagtg ggctttctgc attgctccat
     3061 aatatgccgg tcaaaacctt aggtagagcc aattatgatt ttgagggatt aaccgataaa
     3121 cagccattag atactttttg ggctaatcct cagccaccta attcggaagt atttaaaggc
     3181 tacagacaat ttcatttaca taaaacgcat attaacggta atttttacca taaatcgatt
     3241 ttgcgttatc cttataattg ggaaaatgaa gcacacaggt gatggaaaat attaaaataa
     3301 gtaatatctt atagctaaat tattgtgtta ttattacacc tattgttaaa agagggggca
     3361 ttatggctat ttatgcaata tggaataata aaggtggggt tggtaaaagt tatttaactt
     3421 ttcaaatcgc ttgtgagtat gcaaaacaat acccagcaaa aaaagttctc gttgttgatt
     3481 tatgtcctca agcaaattca tcatctatgc tattaggcgg cattgaaaaa ggtgagcaag
     3541 agttagcaaa aattcattct caagtaaaca agaatactat ttcaggatat atttcagaaa
     3601 gattagtaag tccttaccgt tctattaata gtgcaacgaa atatgtaact caagttagca
     3661 ctataaatga atatgtacca aaaaatgttt atcttgttgt tggtgatgag gaattagaaa
     3721 tccaatcatc tggtatttta catgcttccc aagctgttca tgttcctgat tcttggcggc
     3781 tagtgcatct ttgggtgcgt gatttagttt ctgatataca gaacgcttgg gataatgatg
     3841 ataacgttgt atttattgat tgcaatccaa gtttcactat ttatactgaa ttagcgttgg
     3901 ctgcttcaga tagattgatc atcccttttt ccgctgatgg ttcatcaaaa cgagctgtta
     3961 agtctgtatt atctctagta tatggggtta aaaggcactc tggggatgtc agctctcatt
     4021 tttatacaga atctcaaaag agtaagttag cattacctca aatttatatg tatgtaggta
     4081 atagattaac gataaatgcc aaagcatcag caaccgcatt taaaacagtt gtagaggaaa
     4141 taggtgatga aatctataat atatggaaaa ctaatgctaa tttgttctgt attcacccag
     4201 caggtgttgg tactcctaca aataaatcaa gcttcagaaa aatgtttcag tatgaggtga
     4261 gagatgcaaa tacagcatct gttgtttcag gtgctttagg tattccaatt agtcgcttat
     4321 catcaggaaa aagagatgtt ttaggtaaaa atgtaatggt gaatcagtca caattagatg
     4381 ctcaagttcc taacattcaa a
//
LOCUS       DANLXS010000186         4397 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.187, whole genome shotgun sequence.
ACCESSION   DANLXS010000186 DANLXS010000000
VERSION     DANLXS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.187"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1147
                     /locus_tag="PXD71_002289"
     CDS             <1..1147
                     /locus_tag="PXD71_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDL6292360.1"
                     /translation="YKIALLFNANKIYDRGVMQGIGQFIQASQCTWDIFVEDDFVYHK
                     NSIESLSIDGIIADFDDLETVELLKDIDVPIVAVGGSYQNPDYYPDLPYVATDNYALV
                     EAAFLHLKQKGINQFAFYGYPAETEKHWAIERKNAFIQLMEKYEHHPNCYIGDQFHSD
                     NWVELQAKLGEWLQTLPAHTGIIAVTDARARHLLQTCEHLKIAVPEQLCVIGIDNEEL
                     IQYLSRVSLSSVTQGTSQIGYHAVKLLYRKLNNLPVSTKPILIPPVKVEERRSTDYRS
                     LQDPMVIQAMHFIRHRACQGIKVEQVLDHLKISRSNLEQRFKQEMNKTIHQVIHEEKL
                     ARATYMLKFTEISTQEIANVCGYPSLQYFYAVFKKEFGKTPKEFRQE"
     gene            1240..2239
                     /gene="gntR"
                     /locus_tag="PXD71_002290"
                     /pseudo
     CDS             1240..2239
                     /gene="gntR"
                     /locus_tag="PXD71_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674159.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
     gene            complement(2293..2808)
                     /locus_tag="PXD71_002291"
     CDS             complement(2293..2808)
                     /locus_tag="PXD71_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL6292361.1"
                     /translation="MANGKAFILMGVSSTGKTSVGTAVAQRLGIKLIDGDDLHPRANI
                     LKMASGTPLNDADRTPWLERINDAVFSFEQKSEKGIIVCSALKKKYRDQIRQGNADVT
                     FLFLHGSFELVLARMQQRKGHFMKPEMLQSQFNTLEIPQADEPDVIFIDIDGSFDEVV
                     ERCVKAVEPLL"
     gene            2995..4344
                     /locus_tag="PXD71_002292"
     CDS             2995..4344
                     /locus_tag="PXD71_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL6292362.1"
                     /translation="MLLFIMFAAIILLLVLIMKFKVHAFVALIIVSLLTALAAGIPVD
                     KILPTLLNGFGSTLASVALLVGLGAMIGRLLEITGGAKVLADTLINKFGKERAPFALG
                     VASLLFGFPIFFDAGLVVMLPIIFSVAKQFGGSVLRYAFPSAGAFAVMHAFLPPHPGP
                     VASGDLLGVNMGLLVLVGLVCAIPTWYIGTYLFSQFISKRIHVDLPKAFLNGLSANEM
                     AVQNPPSFGKVLTVLVLPIILILFDTGLNTLSVAKVIDGSEMWVQSLRLIGKTPVALL
                     ITLIVAIMLLRDNRSFDQIEKICNNALGPICSIVLVTGAGGMFGGVLRASGIGDVLSE
                     MMKDTGMPIVVAAFIIATVFRVAQGSATVALTTTAALIAPTVAAATDLSQFDLCFIVI
                     AIASGATVLSHVNDSGFWLMSRFLEMDEKTTLKTWTMLETAIGLTGFACALIGSWLL"
BASE COUNT         1153 a         1015 c          986 g         1243 t
ORIGIN      
        1 ttataaaatt gccttacttt ttaatgctaa caaaatttat gacagaggag taatgcaggg
       61 cattgggcag tttattcaag catcacaatg tacttgggat atttttgtgg aagatgattt
      121 tgtctatcat aaaaatagta tcgaaagcct gtcgattgac ggcattattg ccgattttga
      181 cgatttggaa acagttgagc tacttaaaga tattgacgtc cccattgtag cagtaggcgg
      241 ttcttaccaa aaccctgact attaccctga ccttccttat gttgctaccg acaattacgc
      301 cttagttgag gcagcatttt tacacctaaa acaaaaagga atcaaccaat ttgcgtttta
      361 cggctatcct gcagaaacgg aaaaacattg ggcgatagag cgaaaaaatg cctttattca
      421 gttgatggaa aaatatgaac atcacccaaa ttgttatatt ggtgatcaat ttcattcaga
      481 taattgggtt gaattacaag ccaaattagg cgagtggtta caaaccttac cagcccacac
      541 gggcattatt gccgttactg acgcaagggc aagacacctg cttcaaacct gtgagcattt
      601 aaaaatcgct gtacctgaac aactttgcgt gatcggcatt gataacgaag agttgattca
      661 atacctctct cgggtttctc tttcttcagt cacacaagga acatcacaaa tcggctatca
      721 cgctgtgaag ttattatatc gaaaattaaa taatctaccg gtttcaacca agccgatttt
      781 aatcccgccg gtaaaagtgg aagaacgccg ttcgaccgat tatcgttctt tgcaagaccc
      841 aatggtaatc caagctatgc actttattcg tcaccgagct tgccaaggca ttaaagtaga
      901 gcaagtactg gatcatctta aaatttcccg ctctaacctt gaacagcgtt ttaagcagga
      961 aatgaataag acaattcacc aggtgattca cgaggaaaaa ttagcaagag caacatatat
     1021 gctcaaattt accgagattt cgacccaaga aattgccaat gtgtgcggtt atccctcttt
     1081 acaatatttc tatgccgtgt ttaaaaaaga gtttggtaaa accccgaaag agttcaggca
     1141 agagtagtaa caagcggtca atttcaccga atattttgca attattgcct tatcttcagt
     1201 tataatcagc gaaattaaat tttttcgtta tctattccaa tgagaaaacc gaaacgccca
     1261 actttgcaag acatcgctac ccatctcggc attaccaaaa tgacggtcag tcgctttttg
     1321 cgggatccaa acaccgttgc caaggagacc ggcgagcgga ttgcacaagc gattgagctg
     1381 tttggctata tcccaaaccg tgcaccggat attctctcga atgccaaaag ccgtgcaatt
     1441 ggcgtgctgg ttccttcgct taccaatcaa gtgtttgccg atgtgattaa aggcattgag
     1501 cagattaccg accaagccag ctatcaaacc atgctcgccc actatggtta cagcgaacaa
     1561 aaggaagagc agcggattga gtcgctgctt tcgtacaata ttgacggcat tatcttatcg
     1621 gaaaatcacc actcgccccg caccttgaaa atgctggaag tggcgaatat tccggtgatt
     1681 gagataatgg aaagcggcga atgtggcatt caacaagcgg tcggatattg acaatatttc
     1741 tgcggctcaa gcaatggtcg agacaatgat tcatcgaggc tgccgacagg tggtgtattt
     1801 cggggcgaga atggataaac gctctcagct gaaaatgcag ggctacgaac aggcaatgct
     1861 caaacacggc ttaagcccac acagcattac tactgcagag cattcttctt tcacgcttgg
     1921 cgcaagccag ctaaaacaag ccctgcaaca cattccgcag ctagacggca ttttttgcac
     1981 caatgacgat ttggcaatcg gggcattatt tgagtgccaa cggttaggca ttcatgtgcc
     2041 gcagcagatt aaaatcgccg gctttcacgg acacgatgtc gggcaatccc tcacgccaca
     2101 gcttgccagc gtcatcacac ctcgccttga aataggcaga gttgccgccc aagagctgct
     2161 caaccgtatt cacggcgtac cacaacaaag ctccataatt aacttgggct atcagatcca
     2221 tttgggggaa agtgtgtagc ccggacaagc ggttaaattt gcaaaaaaat ccgcaaaatc
     2281 caccacttgt gattaaagca acggctcaac tgctttcacg caacgctcca ccacctcgtc
     2341 aaaactgccg tcaatatcaa taaatatcac atctggttcg tctgcctgcg gaatttccag
     2401 cgtattaaat tggctttgaa gcatttccgg tttcataaaa tgccctttgc gttgttgcat
     2461 tcgagccagc accagttcaa acgagccgtg caagaataga aaagtcacat cggcattgcc
     2521 ttgtcggatc tggtctcggt actttttctt caatgctgag cagacgataa tccctttttc
     2581 gcttttttgt tcaaaactga acactgcatc gttaatccgc tccaaccacg gtgtgcgatc
     2641 tgcatcattg agtggcgtgc cacttgccat tttcagtata ttggcacgag ggtgtaagtc
     2701 gtccccatca atcaatttta ttcccaaccg ctgtgcgact gccgttccca cgctggtttt
     2761 gccggtgctg gacacgccca tcaaaataaa tgctttgccg tttgccattg aatttccttg
     2821 tgttactggt aacgttaact tagccgttaa tgtaacaggt tatgctgaaa aatcatctgc
     2881 tatttccaga aaacttgaag tagatcacat ttttaaaata aaagccctgc aaccgtttac
     2941 tctaaactgc tttttggtta tgttaccgat aacaattcaa ccttaggaga tcctatgtta
     3001 ctgttcatta tgtttgctgc catcatttta ttgctggtgc tgattatgaa atttaaagtc
     3061 cacgcttttg tggcattgat tattgtgagc ctgctcactg cattagctgc cggtattccg
     3121 gtagataaaa tcctgccaac attgttgaat ggttttggta gtacgctggc atcagtggcg
     3181 ttactggtgg ggcttggggc gatgattggt cgcttgcttg aaatcaccgg cggggcgaaa
     3241 gtgcttgcgg atacgcttat caataaattc ggcaaagaac gtgcgccttt tgcgttgggc
     3301 gttgcctcgc tgttattcgg cttcccgatt ttctttgatg ccggcttggt ggtgatgtta
     3361 ccgattattt ttagcgtggc aaaacagttc ggcggttcgg tgttgcgcta tgcgtttccg
     3421 tctgccggtg cgtttgcggt aatgcacgcc tttctacctc cgcaccccgg cccggttgcc
     3481 tccggcgatc tgcttggtgt gaatatgggg ctgttagtct tggtgggctt ggtttgtgca
     3541 atcccaacgt ggtatatcgg cacttacctg ttcagccaat ttatcagcaa acgcattcac
     3601 gttgatttgc cgaaagcgtt tttaaacggc ttatcggcaa acgaaatggc agtacaaaac
     3661 ccgccaagtt ttggcaaggt actcacggtg ttggtgctgc cgattatcct gattttgttt
     3721 gataccggct tgaataccct ctccgttgct aaagtgattg atggcagtga aatgtgggtg
     3781 cagagccttc gcttaatcgg caaaacccct gttgctttac tgattacctt aattgtggcg
     3841 attatgctgt tgcgggataa tcgcagtttc gaccaaatcg aaaaaatctg taacaatgcc
     3901 cttggcccaa tctgctcgat tgtcttagta accggtgcag gcggaatgtt tggtggcgtg
     3961 ttgcgtgcca gcgggattgg cgatgtatta tcggaaatga tgaaagacac cggaatgccg
     4021 attgtggttg ccgcctttat tattgctacg gtattccgtg tcgcacaagg ctcggcaacc
     4081 gttgctttaa ccaccacagc agcgttgatt gcgccaactg tggcggcagc gactgattta
     4141 agccagttcg acctctgttt tatcgtgatt gctatcgcct ccggtgcgac ggtgctttcc
     4201 cacgtcaatg actccggttt ctggctgatg agccgtttcc ttgaaatgga cgaaaaaact
     4261 accctcaaaa cgtggacaat gcttgaaact gccatcggtt taaccggttt tgcctgtgca
     4321 ttgattggca gctggttact ctaattccaa ccctgacaag cggtctgttt tcttctaaaa
     4381 tttgcaaaat ttggcag
//
LOCUS       DANLXS010000187         4390 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.188, whole genome shotgun sequence.
ACCESSION   DANLXS010000187 DANLXS010000000
VERSION     DANLXS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.188"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1081)
                     /locus_tag="PXD71_002293"
     CDS             complement(<1..1081)
                     /locus_tag="PXD71_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6292363.1"
                     /translation="MGIMSKSISIFNHKGGIAKTTTAFNVGWSLANQGYQVLLVDLDS
                     QCNLTGLVLGYDQCKEDSDLALFYNNRHHLTMEGIVEALINGQSPDNYLSGNVGQITQ
                     TLNPNLFLLPGHLNVSELDSQISVSLKIAAGVPATRNIPGNLPTLIKKVAEQNNIDYI
                     LYDLSPNVGGLNEVILMSSDYFIVPTSPDFFCWQAVGSLAKNIRKWHNELKAFKELNG
                     FNQSSYAIKNKPQFLGTIQQRYRPRKGQPAKSFEKWIERIRNEVITNFVPVLKTIDCA
                     IDDTAIYAALKSTNSDLEPFDLAHIADFNSLIAISQELRVPVFSLTKEQIKNSGQFGH
                     ALNTMDESKENFDQEFQSLAERIIQL"
     gene            complement(1146..1577)
                     /locus_tag="PXD71_002294"
     CDS             complement(1146..1577)
                     /locus_tag="PXD71_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292364.1"
                     /translation="MKKMAIVALSAFFSMNAFANNNVVYSCTTTDNQTLKVTKEGGNY
                     VYSHGNTTFKNPVKEALKNPASEIAGGSQFTTISLELRNAGKSYIVGHIEADPKSPFE
                     ASVQIQDIKTGNDITSFECRSDKPIRHNFDRKLMRKSGFAA"
     gene            complement(1581..3683)
                     /locus_tag="PXD71_002295"
     CDS             complement(1581..3683)
                     /locus_tag="PXD71_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL6292365.1"
                     /translation="MKKAKSLKFLTACIMLTTMSGYANSNTVNVGNSISNFIENTTGY
                     RTGNLGVSGNNALTEYYPVLSDYMHYSIGGGSAISAPASRGRPNQYGIGIGWKSNLTC
                     GNFDIKLSIKNQLNGITEGFKDLYGNIIESATGAVASLPAMIIQRANPQLYDILSNGL
                     YQGKLDFNNFKTSCEAMSNQLADLAMDSKLAKMAGLHRAAEIAETEPDAMAAKKKMDK
                     KDAEKGIPWVGGEQRGGKNQKPMAIVADIVGAGYNLALGRGPLEKGDIPIASCDGMLC
                     TEWKNPSEAAAYASEVIGETFLDACETCEQSSSGAKAGKGIAHFIEQTTIEKATKLEA
                     ALNHLDSLTPEQLNSLSTSTVSVTKGVLEAIAASPDAALLGARLSQELAISKEMEKLL
                     LIRRAMLAGMREPNVIKSSLVEEAERNLKLLDREIEQVKLEMELQRSINGNTALAAIT
                     QQMNEQALAIDDNAMGDGNRRFANLSNQAGANNLNTSGIANSAQNAVGDRYIAIPKSD
                     GSGMSDFYGAYKASPTNIVSNVNVGSYTQIAQMNGNANEQATALIKQFEGYLSHGKWD
                     VNANRAGYGSDTYTTADGMVHRVQKGTYVSREDAERDLARRMPQFMNVARGNVGADVW
                     AKLTPQAQAALTSVSYNYGSLSKLESVQKAAAVSAQSGDMTALANSIRNLQGHNNGVN
                     ARRRNQEADYIINAPKGN"
BASE COUNT         1223 a          872 c          834 g         1461 t
ORIGIN      
        1 tcagttgtat aattcgttct gctaatgatt gaaattcttg gtcaaaattt tctttacttt
       61 catccattgt attcaaagca tgtccaaatt gacctgagtt tttaatttgc tcttttgtta
      121 aggaaaatac gggaactcgt agctcttggc taatagcaat caacgaatta aagtcggcta
      181 tgtgagccaa atcaaatggc tctaagtcag aattcgttga tttaagagca gcataaatag
      241 ctgtatcatc aatagcacaa tcaatagttt ttaataccgg cacaaaattc gtaatgactt
      301 catttctaat tcgctcaatc catttttcaa atgatttggc cggttgccct tttcttggtc
      361 gataacgttg ctgaatagta cctaaaaatt gtggcttatt tttaattgca taagatgatt
      421 ggttaaaacc attaagttct ttaaatgctt ttaactcatt atgccatttg cgaatatttt
      481 tagcgagcga accaactgct tgccagcaga aaaaatcagg tgatgtaggt acaataaagt
      541 aatcactaga cattaggata acttcattta atccacccac atttggacta agatcataga
      601 gtatgtaatc aatattgttt tgttcagcta ctttcttaat taaagtcgga agattgccgg
      661 gtatatttct tgttgctgga acaccggcag caatttttaa tgatacacta atttgcgaat
      721 caagctcgga aacattaagg tgaccaggta acaagaacag atttggattc agggtttgag
      781 tgatttggcc aacatttcca ctaaggtagt tatcagggct ttgtccgttt atcagtgctt
      841 caacaatacc ttccatagtc aaatgatgtc tattattgta aaaaagcgct aaatcactat
      901 cttctttgca ttgatcgtag ccaagtacca gaccggttag gttacattgt gaatccaaat
      961 cgactaacaa tacttgatag ccttgatttg ctaaactcca accaacatta aatgcagttg
     1021 tagttttagc aatacctcct ttatgattaa aaatactgat agacttactc ataataccca
     1081 taattctatg aaataattaa cccaccgaac tcatttgaga atggtgggtt aatgctttgc
     1141 tttagctagg ctgcaaatcc agattttctc atcagtttgc gatcaaagtt gtggcgaata
     1201 ggcttatcgg aacgacattc aaatgatgta atatcatttc ctgttttaat atcctgaatc
     1261 tgaacacttg cttcaaaagg tgatttaggg tctgcttcta tatgacctac aatataagat
     1321 tttcctgcat ttcttaattc gagcgagatt gttgtgaatt gtgaaccacc tgcaatctcc
     1381 gatgccggat ttttgagagc ttcttttaca ggatttttga atgtggtatt accgtgagag
     1441 tacacatagt ttcccccctc tttggtaact tttaacgttt ggttatctgt agttgtgcaa
     1501 gaatacacta cattgttatt tgcaaaagca ttcattgaga aaaatgctga taaagctaca
     1561 attgccattt ttttcatagg ttagtttcct tttggtgcgt tgataatgta atcggcttcc
     1621 tggttacgtc ttcgagcatt aacaccatta ttatgccctt gtaaatttcg gattgagtta
     1681 gctaaagccg tcatatcacc tgattgtgcg gagacggcag ccgctttttg aacactttct
     1741 agtttagata atgacccata gttgtatgat accgacgtta atgctgcttg tgcttgagga
     1801 gtcaatttag cccaaacgtc ggcaccaaca ttaccccttg caacattcat aaattgaggc
     1861 attcttcgtg ctaaatcacg ttcagcatct tcccggctta cataagtgcc tttttgaacc
     1921 cgatgaacca taccatctgc agtagtatag gtgtcagagc catatccggc acggttagca
     1981 tttacatccc atttcccgtg gcttaaatat ccttcaaact gtttgataag agccgttgct
     2041 tgttcattcg cattgccgtt catttgggca atttgggtat agctccctac attcacgtta
     2101 ctgacaatgt ttgttgggga ggctttatat gctccataaa aatcactcat gcctgagccg
     2161 tctgatttgg ggatagcaat atatctatcg ccaacggcat tttgtgcaga attagcaatg
     2221 ccactggtgt tgagattatt tgctccggct tgattgctta aattggcaaa gcgacggttt
     2281 ccatcaccca tcgcattatc atcaatagca agagcttgtt cgttcatctg ttgagtaatt
     2341 gcagctaatg ctgtattacc gttgattgaa cgttgcagtt ccatttcgag tttaacttgc
     2401 tcaatttctc tgtcaagtaa tttgaggttt ctttctgctt cttctactaa ggaagatttt
     2461 ataacatttg gctcacgcat accggctaac attgctcggc gaatcagtag caatttttcc
     2521 atttctttag aaatagctaa ttcttgcgat aaccttgcac ccagcaaggc tgcatccggc
     2581 gaagcagcaa tggcttctaa tacgcctttt gttacactga ccgttgatgt actcagtgaa
     2641 ttgagttgtt ccggcgttaa actatctaag tgattcagtg ccgcttctag ctttgtggct
     2701 ttttcaatag ttgtttgttc aataaaatgg gcgattcctt tcccggcttt agctccactt
     2761 gaactttgtt cacaagtttc acaggcatca aggaatgttt caccaatcac ttcacttgca
     2821 taggcagcag cctcgcttgg atttttccat tccgtacaaa gcataccatc acagcttgca
     2881 ataggtatat ctcctttttc taatggtcct cgtcctaaag cgaggttata tccggctcca
     2941 acaatgtcag caacgatagc cattggtttt tgatttttac caccacgttg ttcgccacct
     3001 acccaaggga tgcctttttc agcatctttt ttatccattt tcttcttggc tgccattgca
     3061 tcaggctctg tttcagcaat ttcagcggct cggtgtaatc cggccatctt tgctaattta
     3121 ctgtccattg ctaaatcagc aagttggtta gacatggctt cgcaactggt tttgaagttg
     3181 ttgaagtcaa gttttccttg atatagacca ttagaaagaa tgtcatacag ttgaggattt
     3241 gctcgttgga taatcatcgc cgggaggctg gcaactgcac cggttgctga ctcaataata
     3301 ttgccataaa gatctttaaa accttcagta ataccattaa gctggttttt aatggaaagt
     3361 ttaatatcaa agttgccaca agttaaattc gatttccagc caatgccaat cccatattga
     3421 tttggtctgc cacgggaagc tggtgcggaa atggcactac ctcctccaat ggagtagtgc
     3481 atataatcag aaaggacagg gtagtattcc gtgagagcgt tattaccaga aacgcctaga
     3541 ttaccagtcc tatagcctgt tgtattttca ataaaattac taatgctatt gccaacatta
     3601 acggtattac tatttgcgta tccgctcatg gttgtgagca ttatacaagc ggttagaaat
     3661 ttgagacttt ttgctttttt catttattta tcctaatagt gctgataatt taatgagtat
     3721 gtgtactttt ccagtccaca tgacctaaaa aaacttgccc ttgtttttta cagcaagaat
     3781 agggcctcca taatgcccag ctgtaattct cagtattgga gaaactatct gaatgggtag
     3841 cccaatcacc actgtgcgga aagactttag ggtaggattc ggaagtaaat accttaactg
     3901 ttacatctga gggtttatta gcaggactag cacttgccat agctgaaagc agtagtagtg
     3961 gtaaatagta aaatgtctta gcttgcataa atgctccaca aatagagtaa ttcacgttta
     4021 ccttaaaaga tgatttggtt agaagaaatc ggaaattgtt ttttggtttt ggagagtttt
     4081 tgttagtata taagttaatg taacaaggga gagtggatgt atgagcaata gaatacctaa
     4141 agcctgttat gcttacccaa aatcgccgaa caacccgttt aatagattgt cggttatcaa
     4201 tctataccag ccgaaatgtc tcccagcatt ctgaaaggga actctgtaaa attacaggaa
     4261 tgccacctaa acaataaatg gataatgcca gccactttat ccgttgtatt ttgtcacatg
     4321 cgtgattgca atgtcataat attacccgac ttggggatat tatgacattg caatcattgc
     4381 tcacttttct
//
LOCUS       DANLXS010000188         4354 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.191, whole genome shotgun sequence.
ACCESSION   DANLXS010000188 DANLXS010000000
VERSION     DANLXS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.191"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            86..1504
                     /gene="leuC"
                     /locus_tag="PXD71_002296"
     CDS             86..1504
                     /gene="leuC"
                     /locus_tag="PXD71_002296"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDL6292366.1"
                     /translation="MQQAKTLYQKLFDSHVVYEAEGETPIIYINRHLIHEVTSPQAFD
                     GLRVAGRQVRQVSKTFGTMDHSISTQVRDVQQLEGQAKIQVLELEKNCEETGIPLFDI
                     TKKQQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     KQARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGYVVEFCGEAIRDLSMEGR
                     MTVCNMAIEFGAKAGIVAPDETTFAYLKDRPNAPKGQDWEDAIEYWKTLKADEGAVYD
                     AEVILEAKDIAPQVTWGTNPGQVIGIDDVIPNPEDMADPVTRASAEKALAYIGLEAHT
                     DMKDVPVDQVFIGSCTNARIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKE
                     GLDKIFIEAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSP
                     AMAAAAAVFGKFMDIRKVSLNA"
     gene            1517..2119
                     /gene="leuD"
                     /locus_tag="PXD71_002297"
     CDS             1517..2119
                     /gene="leuD"
                     /locus_tag="PXD71_002297"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDL6292367.1"
                     /translation="MAGIKQHSGLVVPLDAANVDTDAIIPKQFLQAITRVGFGKHLFH
                     EWRYLDAEETQINPDFVLNFPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIA
                     PSFADIFYNNSLNNHMLPIKLTEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGEKVYT
                     FELDEFRRHCLLNGLDNIGLTLQHEAAIAAYEKNIPAFLR"
     gene            complement(2191..2655)
                     /locus_tag="PXD71_002298"
     CDS             complement(2191..2655)
                     /locus_tag="PXD71_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292368.1"
                     /translation="MNKFKIALMATVAIGLFACTPSPQSNSTKQQAQVGYLKQNISQA
                     ELNNPKDFKRYSYYCKNFATGSSSYLATYFPLWSESRKQENFGIYFQLDGGKVYPFDH
                     LANISLNARATKFEVRYRSYQPIEGSFVELVAREFTSTYYKNNVPWLECREG"
     gene            complement(2679..3185)
                     /locus_tag="PXD71_002299"
     CDS             complement(2679..3185)
                     /locus_tag="PXD71_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292369.1"
                     /translation="MADPHIQSPMDFWDYLTVIIYRSGFVLATLMLLLLPYYPQTAQY
                     GLLVSGTMLASSLHLYAKSFRLIFQFSVWIGLLGAVFNFPLLAFGAMLLVIGGLSYKE
                     YFCFRVFGLNFQPLLVAVLWIAFALEWSLLVQILSLVCGLLLLVLSIQKWRMPLHFDI
                     GDKSKYQI"
     gene            complement(3208..4185)
                     /gene="dusA"
                     /locus_tag="PXD71_002300"
     CDS             complement(3208..4185)
                     /gene="dusA"
                     /locus_tag="PXD71_002300"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDL6292370.1"
                     /translation="MTNEIQPFYRGRFSVAPMLDWTTRHCRYFHRQFSRQALLYTEMI
                     TAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAKLVAERGYAEINLNVGCPSD
                     RVQNGMFGACLMAKADLVADCIKAMQDAVEIPVTVKHRIGIDDLDSYEFLCDFIEKVQ
                     PYSNDFIVHARKAWLTGLSPKENREIPPLDYDRVYQLKRDFPHLKISINGGIKTIEEI
                     KQHLQFVDGVMVGREAYQNPSMLGEIDSQIFNENRPLVTAKEAVEKMFPYIERELAKG
                     VYLNHIVRHMLGAFQHCKGARQWRRHLSENATKSGAGVEVVEQALAFIG"
BASE COUNT         1351 a          958 c          903 g         1142 t
ORIGIN      
        1 atatcagcaa gcggtcattt ttgcaaaatt ttttgcaaat ttaaccgctt gtattaaaag
       61 ttaccctttt gagttggaac attttatgca acaagccaaa accctatatc aaaaactatt
      121 cgactcacac gttgtgtatg aagccgaagg cgaaacgccg attatttata ttaaccgcca
      181 cttaattcac gaagttacca gcccgcaagc gtttgacggc ttgcgtgttg ccggtcgcca
      241 agttcgccaa gtcagtaaaa ccttcggcac aatggaccac agtatttcaa cccaagtgcg
      301 tgatgtacag caacttgaag ggcaagcgaa aattcaagta ttagaattag aaaaaaactg
      361 cgaagaaacc ggtatcccac tgtttgacat cactaaaaaa caacaaggta tcgttcacgt
      421 aatggggccg gagcaaggct taactttacc cggtatgacc attgtatgtg gtgactcgca
      481 caccgccact cacggggcct ttggtgcgtt agccttcggt atcggtactt ccgaagtgga
      541 acacgtttta gccacccaaa ccttaaaaca ggcccgtgca aaaagtatga aagtggaagt
      601 ccgtggcaaa gtaaacccgg gcattaccgc aaaagatatt gtgcttgcga tcatcggtaa
      661 aacaactatg gctggcggta caggctatgt agtggaattt tgtggcgaag caattcgtga
      721 tctttcaatg gaagggcgaa tgacagtgtg taatatggcg attgagtttg gtgcgaaagc
      781 cggtattgtt gccccggatg aaaccacgtt cgcttacttg aaagaccgcc caaatgcacc
      841 aaaaggtcaa gattgggaag atgccattga atattggaaa accctcaaag ccgatgaggg
      901 tgcagtttac gatgcggaag tgatcttaga agcaaaagac attgctccac aagtgacttg
      961 gggaaccaac ccggggcagg tgatcggtat tgatgatgta attccaaatc cggaagatat
     1021 ggccgatcct gttactcgtg cctctgcgga aaaagcctta gcttacatcg gcttagaagc
     1081 ccatacagat atgaaagatg taccggttga tcaagtcttt attggttctt gtactaatgc
     1141 tcgtattgaa gatttgcgtg ctgcggcagc agtaatgaaa gggcgtaaaa aagccgacaa
     1201 tgtaaaacgt attctcgttg tgccaggttc aggtttggta aaagagcaag cagaaaaaga
     1261 gggcttggat aaaatcttca ttgaagccgg tgcagaatgg cgtaacccgg gttgctcaat
     1321 gtgtttggga atgaacgatg accgcttggg cgaatgggaa cgttgtgcct ctacgtctaa
     1381 ccgtaacttt gagggcagac aaggacgtaa cggtcgtacc cacttagtca gcccggcaat
     1441 ggcggctgcg gcggcggtgt tcggtaaatt tatggatatt cgcaaagtgt cattaaacgc
     1501 ataataggag gaaaaaatgg caggtattaa acaacattca ggcttggtcg ttcctcttga
     1561 tgcagcgaac gtagacaccg atgcaatcat tccaaaacaa tttttgcaag ccattactcg
     1621 agtcggcttt ggtaagcacc ttttccacga atggcgttat ttagatgcgg aagagactca
     1681 aatcaacccc gatttcgtac tgaacttccc acaatatcaa ggtgcaacca ttttactcgc
     1741 ccgtaaaaat ttaggctgtg gctcttctcg tgagcacgcc ccttgggcat tggcagatta
     1801 cggttttaaa gtaatgatcg ccccaagttt tgcggatatt ttctacaata acagcttaaa
     1861 taaccatatg ttgccgatta aattaaccga agaggaagtc gaagagatct tccaatgggt
     1921 gtgggcaaat gaaggaaaac agatccacgt tgatttagaa gcgatgactg tgactgttgg
     1981 cgagaaagtt tatacctttg agttagacga attccgccgc cactgcttat taaacggttt
     2041 ggataatatc ggcttaaccc tacaacacga agctgcgatt gctgcttatg agaaaaacat
     2101 tccggcattt ttacgctaat taaaatacaa aaaaacggct aacatcttta aggcgttagt
     2161 cgttttcttt ttagccattt tctttggcaa ttagccttct cggcactcta accaaggcac
     2221 attatttttg taataagttg aagtaaactc tcgtgccact aactctacaa aactgccttc
     2281 aatcggctga taagaacgat aacgcacttc aaattttgtt gctcttgcat tcaaactgat
     2341 gttggcaaga tggtcgaacg gataaacctt tccgccatca agttgaaaat agataccaaa
     2401 attctcttgc ttacggcttt cagaccaaag tggaaaatag gttgccaaat atgaactgct
     2461 gcctgttgca aaattcttac aataatagct ataacgctta aaatctttcg gattattcaa
     2521 ttccgcttgg ctgatattct gtttcaaata tccaacctgt gcttgttgct tagttgagtt
     2581 tgattgtggt gagggcgtac aagcaaataa gccaattgcc actgttgcca ttaaagcaat
     2641 tttgaattta ttcataaaaa tcccgataaa atattaaatt aaatctgata tttgctttta
     2701 tcgccaatat caaaatgcag cggcattcgc catttttgaa tactcagcac aagaagcaac
     2761 aagccacaga caaggcttaa aatttgcacc aataaagacc attctagtgc aaaagcaatc
     2821 cacaataccg ccactaaaag cggttggaaa tttaagccga atactctaaa acagaaatat
     2881 tctttatagc ttaatccgcc gatcaccagt agcattgccc cgaacgcaag caaagggaaa
     2941 ttaaacacag caccaagtaa gccaatccaa acagaaaatt ggaaaatcag gcgaaacgat
     3001 ttggcataaa gatgcaaaga agatgccaac attgtgccag atactaataa accatattgt
     3061 gccgtttgcg gataataagg caataaaagc aacatcaatg tagctaacac aaaaccgctg
     3121 cggtaaatga tgacggttaa ataatcccaa aaatccattg gcgattgaat atgtggatct
     3181 gccataactg cctccttaaa caataaatta gccaataaac gcaagggctt gttccactac
     3241 ttctacaccg gctcccgact tagtggcatt ttcacttaaa tgacgacgcc actgcctcgc
     3301 tcctttgcaa tgttgaaacg caccaagcat atggcgaacg atatgattta aatagacacc
     3361 tttggcaagc tcacgttcaa tataagggaa catcttttcc accgcttctt ttgccgttac
     3421 aagcggtcga ttttcattaa aaatttgcga atcgatctcg cctagcattg acgggttttg
     3481 ataggcctca cgccctacca ttaccccatc aacaaactgc aaatgctgtt taatttcctc
     3541 aatggtttta atgccgccat tgatgctaat ctttaaatgc ggaaaatctc gcttgagctg
     3601 ataaacccga tcataatcta aaggcggaat ttcacggttt tccttcgggc ttaaccctgt
     3661 cagccacgcc ttgcgggcgt gtacgataaa atcattgcta taaggttgca ctttttcgat
     3721 aaaatcacac aaaaattcat agctgtctaa atcatcaatc ccaatacgat gtttaaccgt
     3781 gaccggaatt tccaccgcat cttgcattgc tttaatacaa tccgccacta aatctgcttt
     3841 tgccatcaaa caagccccaa acataccgtt ttgcactcgg tctgacgggc aaccgacatt
     3901 tagattaatc tcggcatagc ctcgctctgc caccaatttg gcacaatgag ccaactgtgc
     3961 cgggtcgctt ccacccagtt gcaaagccac cggattctcg ctcggatcat attccaacaa
     4021 atcatatttg gcgtgaataa tcgccggggc ggtgatcatt tcggtataaa gcaaggcttg
     4081 acgactaaat tggcgatgaa aatagcggca gtgacgggtt gtccaatcta acattggagc
     4141 aaccgaaaaa cgtccacggt aaaaaggttg aatttcgtta gtcataaaaa tatcaaaata
     4201 aagcggtaaa aaatcagtaa aaaataaccg cttgtaagcg attcaaaaca agcagccatc
     4261 atactcgaaa aagccagata aacatagttt cctcaaggct tccccatcta tctgcgcact
     4321 tttaaaacaa ccttaaaaca caatatatag tgtt
//
LOCUS       DANLXS010000189         4346 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.192, whole genome shotgun sequence.
ACCESSION   DANLXS010000189 DANLXS010000000
VERSION     DANLXS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.192"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            60..1094
                     /locus_tag="PXD71_002301"
     CDS             60..1094
                     /locus_tag="PXD71_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6292371.1"
                     /translation="MKLKKLSLALSTLLLGSAFANSALAEGRLTVYCSATNELCEQEV
                     QAFGKKYNVKVAFVRNGSGSTLAKIEAEKNNPQADVWYGGTLDPHSQAAEMGLLEPYK
                     SPNLEQIIEKFRDPAKLKGNYSSAVYMGILGYGVNLDRIKKLGIEKVPSTWEDLLDPR
                     LAGEIQIADPQSSGTAYTAIATFVQLLGEEKAFDYFRKLHKNISQYTKSGITPARNTA
                     RGETAIGIGFLHDYAIEKKNGANIEMTAPTDGTGYELGGVSILKGARNLDNAKLFVDW
                     SLSKEAQELSWQKGNAFQVLTNTTAESSPYALDPKNLNLINYDFEKYGSSEERKRLID
                     KWVNEVKLAK"
     gene            1162..3092
                     /locus_tag="PXD71_002302"
                     /pseudo
     CDS             1162..3092
                     /locus_tag="PXD71_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534092.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            3190..4239
                     /gene="fbpC"
                     /locus_tag="PXD71_002303"
     CDS             3190..4239
                     /gene="fbpC"
                     /locus_tag="PXD71_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDL6292372.1"
                     /translation="MNNDFLVLKNVTKSFGKVTVIDNLNLSIPQGKMVTLLGPSGCGK
                     TTVLRLVAGLENPTSGQIFIDGEDVTQSSIQHRDICIVFQSYALFPHMSIGDNVGYGL
                     KMQGVPKEERLQRVKEALELVDLGGFEDRFVDQISGGQQQRVALARALILKPKVLLFD
                     EPLSNLDANLRRSMREKIRELQQRLNITSLYVTHDQSEAFAVSDEVIVMNKGKIMQKA
                     PAKELYLRPNSLFLANFMGESSIFEGTIAGNQININGYQFTLANAEQFELPDGEYLVG
                     IRPEAIHLAEQGNISQLCKIKNAVYMGNHWEIIAEWEGKDLLVNTKPENFYPHIKQAY
                     VHFIEQGVFLLKKEL"
BASE COUNT         1163 a          951 c          969 g         1263 t
ORIGIN      
        1 atggtatttc ttgctatatt gtatagcaat ctatgcgata actcattaag gaaataaaaa
       61 tgaaattaaa aaaattatct ttagcactct cgactttatt actcggttca gcctttgcca
      121 actctgcttt agcggaaggt cgtttaaccg tttattgtag tgccactaat gaactttgtg
      181 agcaagaagt gcaagcattc ggcaaaaaat ataatgttaa agtagcattt gtgcgtaatg
      241 gttcaggcag tacattggcg aaaattgaag cggagaaaaa caatcctcaa gctgatgtat
      301 ggtatggcgg tactttagat ccacattcac aagctgcaga aatgggttta ctcgagccgt
      361 ataaatcacc gaatctcgaa caaattatcg aaaaattccg tgatccggca aaattaaaag
      421 gcaactactc ttcggctgtg tatatgggga ttttaggtta tggtgtgaat ttagaccgaa
      481 tcaaaaaact cggcattgaa aaagtgccta gcacttggga agacttgctc gacccacgcc
      541 tagccggtga aatccaaatt gccgacccac aaagctccgg cacggcttat actgcgattg
      601 cgaccttcgt acaactttta ggagaagaaa aagccttcga ctatttccgc aaattacaca
      661 aaaacatttc acaatatacc aaatccggca tcacaccggc acgcaatacc gcccgtggtg
      721 aaaccgctat cggtatcggt ttcttacacg actatgcgat tgagaagaaa aacggggcta
      781 acattgaaat gaccgcacca acagacggca caggctacga attaggtggt gtgagtatcc
      841 tcaaaggtgc aagaaatctt gataatgcaa aactgttcgt ggattggtcg ctttctaaag
      901 aagctcaaga actctcttgg caaaaaggca atgcgttcca agtattaacc aacaccaccg
      961 cagaatcttc cccttatgct ctagatccga aaaacttaaa cttaatcaac tatgatttcg
     1021 agaaatacgg ctcgagcgaa gagcgtaaac gcttgattga taagtgggta aatgaagtaa
     1081 aattagcgaa atagtgttaa tctttccact gaggggataa gcaattatcc cctctctaat
     1141 tataagaatc tgtgaggtgt tatgcagaat ttttcctttt cccgaccgct tgtttgggtg
     1201 cttatcgggt tactcggttt tctcttgtta ccgtctaaag ccttagatta tgggctgttt
     1261 gactcaacct ctgatgaact gctcgccgca atgggctggg cgtgctgttc gcctttattt
     1321 tcctgtcagc gattgtgctg aaaatcagcc tcggttatgc ggtgttgccg ttgattattg
     1381 ccattatggg gcttgcgacc ccaaatttag caacgctcaa agtaatgcag ggtgaccgct
     1441 ttattatcgg ctcgttactg agcattattc tgctgatttt cttttttatt gtgtatccga
     1501 ccatcgccat ttttgtgtcg atgttctaca acggctcgga atttgtgcca agccaagtgc
     1561 tgacaatttt gcaacaacct tatgtcgttc gagtcatcgg taactcgctc tcggttgccg
     1621 gcacggtggg cgttctctcc accctattcg gcttagcctt tgctctctac accactcgga
     1681 ttgctaaacg ctcggcgttt atcgggcgga ttttctcgat tttacctatc gtcaccccgc
     1741 cgtttgtggt cggtttaggc gtaaccctga tgcttgggcg ttccggctat gttactgaat
     1801 ttttagtgga ttactttggc tttgccaaca attggctgta cggtttcacc ggtattgtga
     1861 tagcccatac cctcgcgctc acgccaatgt cgtttatgat tttggaaggg gcattgaaat
     1921 ccattcaccc atcggtggaa gaagcagctt acaccctgcg ttccaaccgc tatcaagcct
     1981 tttttaacat tattttccca ttgttaaaac cggcattggc aaactccttt ttagtggtgg
     2041 caattcaatc cctcgccgat ttcagtacgc cgttagtatt gggcggtagc tttgatgtga
     2101 ttgcaaccca aatctacttc tacattgccg gttctcaact ggattatgcc tctgctagca
     2161 cgcttggggc aattttgttg gtgttctcct tagccatttt cgtgatccaa tacctctgga
     2221 tcggcaatcg ctcttatgtg acggtgtccg gcaaatccta tcggggtgat gtccaagact
     2281 taccaagcgg tatgaaatgg ctgattattt gcaccttatt gttctgggga accttcaact
     2341 tagtgctgta cggcagtatt ttctacggca gtttcaccgt gaactggggt gtggattata
     2401 ccttaaccct tgataactac atcaagctat tcgggcaagg gtttagcgat ggcggtttcc
     2461 cttcgttaat tcaaaccgtg attttcgctg ccgctgccgc cccgattact gcactattcg
     2521 gcttgctgat cgcctatgtg acagttcggc gtgatttcaa aggcaagaaa accctggaat
     2581 ttttaaccct gctctgcttt gccgtaccgg gaacggtggc aggtgtttct tacattctcg
     2641 cctttaacga tgccccgatt tacctaaccg gcaccggtat gatcatcatt ctctcaatgg
     2701 taatgcggaa tatgcctgtg ggtatgcgag cggcaattgc cggcttaggg cagttggata
     2761 aatcgctgga cgaagcctcg ctctcgctga aagccggttc gttcaaaacg atgctgtttg
     2821 tggtgttacc gctactcaaa ccggcactgc tctccgccct cgtcaccagc tttgtgcggg
     2881 caatgacgac tgtcagtgcg attgtattct tagtcaccgc cgacactcgt gtggcaacct
     2941 cctacattct caaccgtgtg gaagacggtg aatacggcat tgcgattgcc tacggctcaa
     3001 ttttaattgt ggtgatgatg gcgattatcc tgatttttga ctggattgtc ggcgacaccc
     3061 gtatcgcccg ttctaaggcg aaaaaaatgt aaaaagacat ctactctctc cctttatggg
     3121 agagagacag aaaaatactt cgttcagaag tatttttcag agagagggat ataattaaaa
     3181 ggacaatata tgaacaacga tttcttagta ttaaaaaacg tcaccaaatc tttcggcaag
     3241 gtgacggtca ttgataatct caacctcagt attccgcaag gtaaaatggt gacattactc
     3301 gggccgtccg gctgtggtaa aaccaccgta ctgcgtttag tggcagggct ggaaaacccg
     3361 acttcggggc aaatttttat tgatggcgaa gatgttaccc aaagttcgat ccaacaccgt
     3421 gatatttgca ttgtgttcca atcttatgcg ttatttccgc atatgtcgat tggcgataat
     3481 gtcggctacg gcttgaaaat gcagggcgta ccgaaagaag aacggctgca acgagtgaaa
     3541 gaggcgttgg aattggtgga tttaggcggc tttgaagatc gctttgtcga tcaaatctct
     3601 ggcggacaac aacaacgtgt cgccctcgcc cgtgccttaa ttctgaaacc gaaagtgcta
     3661 ctgttcgatg aaccgctgag taaccttgac gctaacttac gccgttcaat gcgggagaaa
     3721 atccgtgaat tacagcaacg tttaaacatt acttcccttt atgtgactca cgatcagtcc
     3781 gaagcctttg cggtttcaga tgaagtaatt gtgatgaaca agggaaaaat tatgcaaaaa
     3841 gctccagcca aagagttata ccttcgccct aattctttat tcttagcaaa ttttatgggt
     3901 gaaagcagca tttttgaagg cacgatagct ggcaaccaaa ttaatataaa tggatatcaa
     3961 tttacgttag caaatgctga acaatttgag ttacccgatg gggaatactt agttggcatt
     4021 cgtccagaag ctattcattt agctgaacaa ggaaatataa gtcaactctg taaaattaaa
     4081 aatgcagttt atatgggaaa tcattgggaa attatagctg aatgggaagg gaaagattta
     4141 ttagtcaata ctaaacctga aaatttttac cctcatatca aacaggccta tgtacatttt
     4201 atcgaacaag gtgtttttct tttaaagaaa gagctataac gaatttatac taaattaaga
     4261 ggaaggtagt tataactatt tagcaatttt gagaaaaata ggccgcatgc aaaatctctt
     4321 gatgacaagc ggtcttttct cgccga
//
LOCUS       DANLXS010000190         4337 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.193, whole genome shotgun sequence.
ACCESSION   DANLXS010000190 DANLXS010000000
VERSION     DANLXS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.193"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            52..381
                     /gene="trxA"
                     /locus_tag="PXD71_002304"
     CDS             52..381
                     /gene="trxA"
                     /locus_tag="PXD71_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL6292373.1"
                     /translation="MSNVIHTTDATFEQDVLKSDVPVLLDFWAPWCGPCRMIGPVLDD
                     LSVDAQFDGKVKIVKMNVDENPSVPAQFGVRSIPFLLLFKNGEVIAQQVGAIPHAQMA
                     EFIKQAL"
     gene            552..1775
                     /gene="hflK"
                     /locus_tag="PXD71_002305"
     CDS             552..1775
                     /gene="hflK"
                     /locus_tag="PXD71_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDL6292374.1"
                     /translation="MSWNESGNQQDPWGKPGQKKAEPKPEQQEQGSQQEPTNNQRNEQ
                     QPPDLEEVFSSLLKKMGGGKGPNNSSNQPSASLGKFLPVVLGLAAVVWVGSGFYTVQE
                     AERGVVTRLGKLNDIVLPGLNWKPTFIDSVTRVNVERVSELNTSGSMLTQDENMVQVE
                     MTVQYRVEDPAKYLFSVSNPDDSLKQATDSALRYVIGHMTMDEILTTGRATVRERTWS
                     TLRDIIKTYDMGLLVTDVNFQYARPPEEVKAAFDDAIKAQEDEQRLIREAEAYARGEE
                     PIARGQAQRTIEQAQAYKEAVVLNAKGEVERLSQLLPEYKASPELTRERLYIQTMEKV
                     MKNTPKVVMDSSGNNLNVLPFDKLMNSSSVIKAEQPAAAQVVTPQVVQPQVQPRTQIQ
                     PQQAEQQEPTRKGRF"
     gene            1778..2665
                     /gene="hflC"
                     /locus_tag="PXD71_002306"
     CDS             1778..2665
                     /gene="hflC"
                     /locus_tag="PXD71_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDL6292375.1"
                     /translation="MRRLLVPILAVVAFVVLQAITIVNEGERGIMLRFNKVHRDSDQK
                     VVVYEPGIHFKVPFIDSLKVLDARIQTLDGQEDRFVTVEKKDLLVDSYVKWRISDFGQ
                     FYTSTGGDYQKAADLLRRKVGDRLRSEIGSRTIKDIVSGSRGELMAGAQKALNAGEDG
                     AERLGIEVVDVRVKQINLPNEVSSSIYQRMRAERDAVAREHRSQGNEKAEVIRAEVDK
                     KVVLILANANKTAQALRGEGDAQAAKLYSEKFGNEPEFYSFIRSLKAYEDSFAEGQNN
                     MMLLKPNSEFLRFMQAPTK"
     gene            2839..4137
                     /gene="purA"
                     /locus_tag="PXD71_002307"
     CDS             2839..4137
                     /gene="purA"
                     /locus_tag="PXD71_002307"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL6292376.1"
                     /translation="MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTL
                     IINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEALMKEMGELEERGINVRERLKISE
                     ACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEHFAA
                     KLKDILDYYNFQLVNYYKVEAVDYQKTLDDVFAIADVITGMVADVTTLLHEARENGDN
                     ILFEGAQGTMLDIDHGTYPFVTSSNTTAGGVATGSGYGPRHLDYVLGIIKAYCTRVGS
                     GPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLD
                     VLDGFKELKICVAYKMPNGKIVEYAPLAAKDWEGVEPIYETMPGWSENTFRVTKREDL
                     PQAALDYIKRIEELVGVPVDILSTGPDRVETMILRDPFAA"
BASE COUNT         1334 a          771 c         1023 g         1209 t
ORIGIN      
        1 acttgccagt tagggcgttt ctttttttat ttaatattta aggataaact tatgagcaat
       61 gtcattcata caacagatgc tacatttgaa caagatgttt taaaatcaga tgtaccggta
      121 ttattagact tctgggcgcc ttggtgtggt ccttgccgta tgatcggccc cgtacttgac
      181 gatttatctg ttgatgcaca atttgatggt aaagtaaaaa ttgtcaaaat gaacgtagat
      241 gaaaatccat ctgtaccagc acaatttggc gtacgcagca ttccattttt actattattc
      301 aaaaatggtg aagtgattgc ccagcaagtt ggtgcaattc cgcacgccca aatggctgaa
      361 tttatcaaac aagcattata attttaagag caatactagg gcgaatttat cgccctattt
      421 ttttgcaaat aaatagtgaa aaaagaccgc ttgtttcaga taaacctagt caaaagctta
      481 ctaaataggt acaatcacta acttgcattc taagctttaa agtgcgattc gattttattt
      541 tggagatatt tatgtcgtgg aatgaatcag gcaatcagca agatccttgg ggtaaaccgg
      601 ggcagaaaaa agctgagcca aagcctgagc aacaagaaca gggttcacaa caagagccga
      661 ccaataatca aagaaatgaa caacagcccc ctgatttaga agaagtattt agcagtttat
      721 taaaaaaaat ggggggtgga aaagggccta ataatagttc aaatcaacct agtgcaagct
      781 tagggaaatt tctacctgtg gttttaggtc ttgcagcagt agtttgggtt ggctcaggtt
      841 tttacactgt gcaagaggct gaacgtggtg tggttactcg tttaggtaag ctgaatgata
      901 tcgtgttgcc agggttgaat tggaaaccga cttttattga ttcggttaca agagtaaacg
      961 tagaacgtgt ttcagagctg aacaccagtg gctcaatgct gactcaagat gaaaatatgg
     1021 tgcaggtaga aatgacggtt cagtatcgtg tggaagaccc ggctaaatat ctgtttagtg
     1081 tgagcaaccc tgatgacagc ttaaaacaag caacagacag tgccttgcgt tatgtgattg
     1141 gtcatatgac gatggatgaa attttaacca cagggcgtgc aaccgtgcgt gaaagaacgt
     1201 ggtcgacatt gcgtgatatt atcaaaacct atgatatggg tttattggta acagacgtaa
     1261 acttccaata tgcacgaccg cctgaagagg tgaaagccgc ctttgatgat gcaatcaaag
     1321 cacaagaaga tgagcaacgc ttaattcgtg aagcggaagc ctatgcaaga ggtgaagagc
     1381 cgattgcacg tggccaggca cagcgtacga ttgagcaagc tcaagcttac aaagaagcag
     1441 ttgtgttaaa tgcgaaaggg gaagtggagc gtttgagtca attattacct gaatataaag
     1501 cctctccaga gctgactcgt gaacgtttat atatccagac aatggaaaaa gtcatgaaaa
     1561 atacgccgaa agtggtgatg gattcaagtg gtaataattt gaatgtattg cctttcgata
     1621 agctaatgaa ttcttcatcg gtaataaaag cggagcagcc tgcggcggca caagtagtta
     1681 caccacaagt tgttcagccg caagtacagc caagaacaca aattcagccg caacaagcag
     1741 aacaacaaga accaactcgt aaggggagat tttaatcatg cgtagattat tagtacctat
     1801 acttgctgta gttgcttttg ttgtgctaca ggcaatcacc attgtaaacg agggcgagcg
     1861 tggcattatg ttacgtttta acaaagttca tcgtgatagc gatcaaaaag tggtggtata
     1921 tgaaccgggg attcacttta aagtaccatt tatcgatagt ttaaaggtat tagatgctcg
     1981 tattcaaact ctagatgggc aagaagaccg ttttgtaaca gtcgagaaaa aagatttatt
     2041 agtggactcc tatgtaaaat ggcgaatcag tgattttggc caattttata cctctactgg
     2101 gggggattac caaaaagcgg cggatttatt acgccgtaag gtaggggatc gtctgcgttc
     2161 cgagattggt tctcgaacca ttaaagatat tgtatccggc tctcgtggtg agttaatggc
     2221 aggagcccaa aaagcgctga atgccggtga agatggagca gagcgtttag gaattgaagt
     2281 ggttgatgtg cgagtgaagc aaatcaactt accaaatgaa gtttcttctt caatttacca
     2341 acgtatgcgt gccgagcgtg atgcggttgc tcgtgaacac cgctcacaag gtaatgagaa
     2401 agcagaggta attcgtgccg aagtggataa aaaagtggtg ctgattttgg caaatgcgaa
     2461 caaaactgcc caagccttgc gaggtgaagg tgacgctcaa gctgcaaaac tctattcaga
     2521 gaaatttggt aacgagccgg aattttacag ttttattcgt agtttaaaag cctatgaaga
     2581 tagctttgcc gaaggtcaaa ataatatgat gttattaaag ccaaacagcg aattcttacg
     2641 ctttatgcaa gcaccgacaa aataattgta tctataaaac ccgatcttgt atcgggtttt
     2701 taattttcat ccctattctg atcataacaa gcggtaaatt ttggctgatt ttttgcaaaa
     2761 aatttgaaaa aattgaccgc ttgcgatatg aagttataat gggtttcgtt attcatccta
     2821 actaaaagag gcatttctat gggtaaaagt gttgctattc ttggcgctca gtggggcgat
     2881 gagggaaaag gcaaaattgt tgatttatta acagatcgtg tgaaatatgt agtacgttat
     2941 caaggtggac ataatgcagg gcatacctta atcattaatg gtgaaaaaac cgttctgcgt
     3001 ttaattccat cgggtatttt gcgtgataac gtgacttgtt taatcggtaa tggtgtagtg
     3061 ctttctccag aggcattaat gaaagagatg ggggagctag aagaacgagg cattaatgtg
     3121 cgtgagcgtt taaaaatttc tgaagcttgc ccgttaattc tgccttatca cgttgcgatg
     3181 gatcacgctc gagaagcggc acttggtaaa aacaaaattg gtacaacagg acgtggtatt
     3241 ggcccagctt atgaagataa agtcgctcgc cgtggtttac gtgtcagcga tttattcgac
     3301 aaagaacatt ttgccgcaaa attaaaagat attttagatt actacaactt ccaattagtc
     3361 aattattaca aagttgaagc ggtagattat caaaaaacct tagatgatgt gtttgcgatt
     3421 gcggatgtga ttaccggaat ggttgccgat gtgacaactt tacttcacga agcacgtgaa
     3481 aacggcgaca atattttatt tgaaggggct caaggcacga tgttagatat cgaccacggt
     3541 acttacccgt tcgtaaccag ctcgaatacg acagcaggcg gtgtggcaac cggatcaggc
     3601 tacggtcctc gtcatttaga ttatgtatta gggattatca aagcctactg tacccgtgta
     3661 ggtagtggac cgttcaccac cgaactattt gacgatgtag gtgctgaaat tgctcgcaaa
     3721 ggtaatgagt tcggagcggt aaccggtcgc cctcgccgtt gtggctggtt tgatgccgta
     3781 gccgttcgcc gagcagtgca aatcaactcc atttcaggct tctgtatgac taaactcgat
     3841 gttttagacg gattcaaaga gctaaaaatc tgcgttgcct acaaaatgcc aaacggaaaa
     3901 attgtcgaat atgcccctct tgcagcaaaa gactgggaag gtgttgaacc aatttatgaa
     3961 acaatgccgg gttggtctga aaatactttc cgtgtgacaa aacgtgaaga tttaccacaa
     4021 gccgcattag attacattaa acgtatcgaa gagctggtcg gtgtgccggt agatattctt
     4081 tctacaggtc cggatcgtgt cgaaacgatg attttgcgtg atccttttgc agcttaataa
     4141 aagataaaat aaagccattt agtgagatag agctagatgg cttttttgtt tgaaccagta
     4201 aactttactt tggcacaatt ttgtcacaat acagaaaata gttctttagc gtgagttttt
     4261 gaggctgtgt aagtatttga atttaaaaga atttaatggc acgccctaaa ggattcgaac
     4321 ctttgaccca cgcctta
//
LOCUS       DANLXS010000191         4282 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.194, whole genome shotgun sequence.
ACCESSION   DANLXS010000191 DANLXS010000000
VERSION     DANLXS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.194"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(116..826)
                     /locus_tag="PXD71_002308"
     CDS             complement(116..826)
                     /locus_tag="PXD71_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292377.1"
                     /translation="MWKKIRIDFTDTVKCQFCSRMITSGKAIVVQNELGNISFSGPSC
                     AQNKNGQNVVNPKEKIVDITKGCLLHEPTTNDKTDKQVDVEQDNFTSIHDDEVHNYLD
                     NNAVNAYLTLRFEKLSHLTSIIKSQKLNEIYHNYELTGQISQKDESYIRVIMYGEAYP
                     LYTYQNLQAVYAAEYWLQSFIQHNPENDLNFIQDTLNQLRRKLTLTEKQIMGINNWFK
                     NSDGKMVKLKTNAFVRGK"
     gene            1303..1821
                     /locus_tag="PXD71_002309"
     CDS             1303..1821
                     /locus_tag="PXD71_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6292378.1"
                     /translation="MRYIKYSLFLVSTMLISGCAELSAINDKVGDWAGQMNQTLYGSK
                     DGSVEQHNGRQVYTKTRNGKYVYQDSGTLQLKRNIDDVFLKVRREFGFRPTNADSDSR
                     KVYHTVQGTFYHVSGYFESDVDTDGVHLGENYLEVIIEKGSKNQVNVEWKATGSARWL
                     DSAKQRLLNTLK"
     gene            1981..4032
                     /locus_tag="PXD71_002310"
     CDS             1981..4032
                     /locus_tag="PXD71_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL6292379.1"
                     /translation="MKLFICEKPSQARDLAGVLGATSKGEGLLATADKSIIVTWGFGH
                     LVEQYQPEDYDEAWKRWAFETLPIIPAQWKMTPKKESKKQYSVVIGLIKKATSVVIAT
                     DADREGEMIARELLDLAGYRGQIQRCWLSALDDASIRKALQTLKSGKETEALYYAGMG
                     RSRSDWLIGMNFSRLFTLMAQAKGYSGKPLSVGRVQSPTLTLVVNRDREIANFVPKSH
                     YALAVQLTTTSGETFVAGLAVPEQYLDESGLCLDSRVIQQAEAQIKQTGLAKVVTVET
                     KREKKSPPLLYALSDLQGECNRLFGFGAQQVLDIAQSLYEEHKITTYPRTDCGYLPES
                     QFSDVPNVIRSLVASDSGLQTLLPKLNLRQKSRAWDDKKITAHHGIIPTIKKADLSKL
                     SEEEMKVYDLIRRRYLAQFLPHLEMDKTVATLQSSGHTLVARGNVIVAQGWRILFGKN
                     VDEETGNDDEKQGLPVLTKNQQCNVTHSEVRTLQTKPPAHYTEGTLLDAMKNAARFVT
                     DPRLKQRLRETEGLGTEATRAGLIQGLIDKGFLLKKKKSLVASAEAVALIDSLPDLLK
                     NPGLTALWEQALNQIAEGSMTLDAFMQKQEAFVKQLIGSCMQQGMSLGNIEIRKCSEC
                     GKPMRKINHAKGTFWGCTGYPDCQHKEADKKVKSASNKSAKKSSNLNVLDQLNKLRSQ
                     L"
     gene            complement(4093..4230)
                     /locus_tag="PXD71_002311"
     CDS             complement(4093..4230)
                     /locus_tag="PXD71_002311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292380.1"
                     /translation="MTLQSRFRQLQQTNVAGIIHLMFSWHSHKVEFPLRMLGDILAGV
                     D"
BASE COUNT         1345 a          815 c          845 g         1277 t
ORIGIN      
        1 tacatataac ttgaatttgt caaaataagc aagcaaataa taggaaagag tcttttaaaa
       61 ccagtcttac gtcgggccta cgtaacaaaa taagtggcca ccacaattat gttaactatt
      121 tccctcttac aaaagcattt gtctttaatt tcaccatttt tccatcagaa ttcttaaacc
      181 aattatttat gcccataatc tgtttttcag ttaatgttag ctttcttctg agctgattta
      241 atgtgtcttg tataaaattt aagtcattct ctggattatg ctgaataaat gattgaagcc
      301 aatattcagc agcataaaca gcttgtaagt tttggtatgt atacaaagga tatgcctcac
      361 catacataat cactcttatg taactttcat ctttttgaga tatttgccct gtaagttcat
      421 agttatgata gatttcattt aacttttgag attttataat cgaggttaaa tgcgatagtt
      481 tttcaaatct taaggtcagg tatgcattaa cagcattatt gtccaaataa ttatgtactt
      541 cgtcatcgtg tatggatgtg aaattatctt gctcaacatc tacttgctta tccgttttat
      601 catttgttgt tggctcatga agtaaacatc cctttgtgat atcaactatt ttctctttag
      661 gattgactac attctgaccg ttcttatttt gagcacaaga cggtcctgaa aaagagatat
      721 tgccaagttc attttgaaca acaatagctt tgcctgatgt aatcattcta ctgcaaaatt
      781 gacatttcac tgtatcagta aaatcaatcc taattttttt ccacataaca tatctctcca
      841 tatagtgagt aataaaaata cttcttaact ctcttcattg atgaaataat tgcaaaaata
      901 actttgatag aggataaata tatgaatttc cctccaactc gatggaaaat attacaccag
      961 tatcaaactg agctagagct ctgggttgaa caagccacag aagagcatat caagcaatac
     1021 ttgactgaaa ttcacggatg tgtataccca gatgactggg aaaatgtgcg tatatcccat
     1081 atgtcatctc aaactcatat tttccatagc tgtgaacgtt gcctagaact tatctctgcg
     1141 atattaacaa tctcacaact aagcagaccc gatctaaatg gtctactgtg tgacagaaag
     1201 ctataatatt ttttctcttt gtaagtaaaa gagttttaag tagattttaa ccgcactttt
     1261 gcaaaaaatg aacgcagata acccgcttgt aaggagaatt ctatgcgtta catcaaatat
     1321 agtttatttc ttgtttctac aatgctaatt tcaggctgtg ctgaactctc agcaattaac
     1381 gataaggttg gggattgggc cgggcaaatg aatcagacgc tctatggctc taaagacggc
     1441 tctgttgaac agcataatgg gcgacaagtt tatactaaaa cccgaaatgg taagtacgtt
     1501 tatcaagatt ccggcacatt gcagttgaag cggaatattg atgatgtatt cctgaaagtt
     1561 cgtcgggaat ttggcttccg cccaacgaat gctgatagtg actcccgtaa agtttatcat
     1621 acggtacaag gtacttttta ccatgtttcc ggctattttg aatctgatgt agatactgat
     1681 ggggtacact taggtgaaaa ctacttggaa gttatcattg aaaaaggcag taaaaatcag
     1741 gtgaatgttg aatggaaagc aactggtagt gctagatggc tcgatagtgc aaaacagcgt
     1801 ttacttaata cattaaaata ataactcata ttgctcctag aacctcttcc cagtggaaac
     1861 actgggattt tttatgtcct tatttctcaa attattacat cctacgagac aataagtcgt
     1921 tgaatgctat ttaatcagca cattactatt tgcctatctg tattcatttt aggaaataat
     1981 atgaaattat ttatttgtga aaagccatct caagctcgag atcttgccgg cgtattgggt
     2041 gctacttcaa aaggtgaagg cttgctggcg actgcggata aatctattat tgtcacttgg
     2101 gggttcgggc atctcgttga gcaatatcag ccggaagatt atgatgaagc gtggaaacgc
     2161 tgggcatttg aaacattacc cattattcct gctcagtgga aaatgacacc gaaaaaagag
     2221 tctaagaagc aatattctgt tgtaataggg cttatcaaaa aagcaacctc cgtggtcatt
     2281 gcgaccgatg ctgatcgaga aggtgagatg attgctcgtg agctgttaga tttagctgga
     2341 taccgaggtc aaatccaacg ttgctggcta tcagctctgg atgatgccag tattcgaaag
     2401 gctcttcaaa cactcaaatc aggtaaagaa accgaggctc tttactacgc cggtatggga
     2461 cgtagccgct cggactggtt gattggaatg aacttctccc gtttatttac cttaatggct
     2521 caagcaaaag gctattccgg taagccattg agcgttggtc gagtacaaag tccaacatta
     2581 actcttgtgg ttaatcgaga tagagaaatt gctaatttcg tgcctaaatc tcattatgcg
     2641 ttagcggttc agctgacaac aacctctggt gaaactttcg ttgccgggct tgcagtacct
     2701 gaacagtatt tggatgagtc cggactttgc cttgatagtc gtgtcataca acaagccgaa
     2761 gctcaaatta aacaaacagg cttagccaaa gtggtgacgg ttgaaactaa acgagagaaa
     2821 aaatctccac cgttactcta cgcattaagt gatttacaag gcgaatgtaa ccgcttgttt
     2881 ggctttgggg ctcagcaggt gttggatatc gcacaatccc tttacgaaga gcataaaatc
     2941 acgacttatc cccgtacaga ctgtggttat ctgccagaat cacaattttc tgatgttcct
     3001 aacgtaatcc gctcattagt tgcctctgat tcagggctac aaactctctt gcctaaatta
     3061 aatttgcgac aaaaaagtcg ggcgtgggac gataaaaaaa ttaccgctca ccacggtatt
     3121 atcccaacca ttaaaaaagc tgatttaagt aaattgagtg aggaggaaat gaaggtctat
     3181 gatctcattc gccgccgata tcttgctcaa ttcttaccgc acttggaaat ggataaaacc
     3241 gttgccactc tgcaatctag tgggcacact ctggttgctc ggggaaatgt catcgttgct
     3301 caaggctggc gtatcttgtt tggcaaaaat gtggatgagg aaactggtaa tgatgatgag
     3361 aaacaaggat tacctgtatt gactaaaaat cagcaatgca atgtcactca ttctgaagtc
     3421 cgaacattgc aaactaaacc tcccgcacac tatacagagg ggacattact cgatgcaatg
     3481 aaaaatgccg ctcgctttgt gactgaccca agactcaaac aacgcttacg tgaaacggaa
     3541 ggtttaggca cagaagcaac ccgtgccgga ttaattcaag gcctaattga caaaggattt
     3601 ctccttaaga agaaaaaatc actggttgcc tcagcagaag ccgttgcctt aattgatagt
     3661 ttgccggact tactgaaaaa tcccggttta acggcattat gggaacaagc cctcaatcag
     3721 attgccgaag gtagtatgac acttgatgca tttatgcaaa agcaagaagc atttgtaaaa
     3781 caacttatcg gcagttgtat gcaacaggga atgtcacttg ggaatattga aatccgaaaa
     3841 tgttcagaat gtggtaagcc aatgagaaaa atcaatcacg ctaagggaac attctgggga
     3901 tgtactggtt atccagattg ccaacataaa gaggctgata aaaaggtaaa atcagcttca
     3961 aataaatctg ctaagaaaag tagtaattta aatgtactcg atcaactgaa taaattaaga
     4021 agccagttat aaattcaagt agattatggt attatcaagg aacaaagaac ccgttttgta
     4081 gattgtcggt tatcaatcta caccagccaa aatgtctccc agcattctga gagggaactc
     4141 cactttgtgg gaatgccaac taaacattaa atggataatg ccagccacat tagtctgttg
     4201 taattgtcta aatcgtgatt gcaacgtcat aatattaccc gattgggggg attatgacgt
     4261 tgcaattact gctaactatt ct
//
LOCUS       DANLXS010000192         4277 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.195, whole genome shotgun sequence.
ACCESSION   DANLXS010000192 DANLXS010000000
VERSION     DANLXS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.195"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            138..2072
                     /gene="iolD"
                     /locus_tag="PXD71_002312"
     CDS             138..2072
                     /gene="iolD"
                     /locus_tag="PXD71_002312"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL6292381.1"
                     /translation="MQTTRLTVAQALVQFLDNQYLDVDGKEIKFVHGVAAIFGHGNVL
                     GIGQALAQNAGGLTLYQGRNEQGMAHLAMGFAKQNLRQRIIACTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDVFATRQPDPVLQQIEQPYDLSISTNDAFRAVSKYWDRVQRPE
                     QLMSACINAFRVLTDPAETGAVTIALPQDVQAESFDFPNSFLQKRVHRLERTPPTTAM
                     IKQAVTLIQTKKKPLIVCGGGVRYSQAGQILQKFAKQFGIPFAETQAGKSAVVSDHAL
                     NVGGVGETGCLAANRLAKEADLVIGIGTRYTDFTTSSKWIFQNPNVQFLNINVARFDA
                     YKLDGVQLVADAKTALEALSQAIPTDYQAQWENEISQAKADFADEIQRLRAVQYHPDF
                     TPEINDNLDHKAVHNEFIQLTGSQLTQTQVLGIVNETIDNNAIIVAAAGSLPGDLQRT
                     WLSKGEETYHLEYGYSCMGYEVSAAVGVKIARPEQEVYALLGDGSYLMAHTELVTSIQ
                     EGKKINVVLFDNMANGCINNLQIGNGMDSFGTEFRFRNGETNQLNGGLVPIDFAKNAE
                     AYGCKTYRVTNEAELRAALDDAKKQTVSTLIDIKVLPKTMAHGYGCWWHVGVAEVSES
                     ERIQQARQRSEEQLAKARKY"
     gene            2199..3095
                     /gene="iolE"
                     /locus_tag="PXD71_002313"
     CDS             2199..3095
                     /gene="iolE"
                     /locus_tag="PXD71_002313"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011817366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL6292382.1"
                     /translation="MKAENIQLGIAPIGWTNDDLPELGAENTFEQCVSEMALAGYTGC
                     EVGNKYPRDVAVLKHKLDVRGIQICNAWFSTFFVDGKREETIAEFIKHRDFLHAMGAK
                     VIGCSEQSRSIQGTTKSVFKEKPIFNDEDWKRMAEGYNELAKLAAEKGMKVCLHHHMG
                     TGIQTPEEVDRYMAEVNDDVYLLFDSGHLYYSEGCQKAMLGVLEKYIDRIIHVHLKDV
                     RDEVMAEVKAKDLSFLEGVKKGTFTVPGDGVIDFRPIFDILEKHDYKGWMVVEAEQDP
                     ALANPFEYALKGRKYIRETAGV"
     gene            3097..4107
                     /gene="iolG"
                     /locus_tag="PXD71_002314"
     CDS             3097..4107
                     /gene="iolG"
                     /locus_tag="PXD71_002314"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL6292383.1"
                     /translation="MLKVGIIGAGRIGRVHSESISKYVKGAEIKAISDVKITEELTAW
                     AKSMGIPEVYDDYKKILQDPEIDAVLVCSSTNTHAPISIEAAQAGKHVFCEKPVDADP
                     AKIREVLSAVEKAGVKFQVGFNRRFDHNFKAIKDRVAAGDIGEPHVIRVTSRDPEAPP
                     IEYVKVSGGMFFDMTIHDFDMIRYLSGSEVEEVFAVGTVLVNPEIGKAGDIDTAVITL
                     KLKNGAIGVIDNSRKAAYGYDQRAEVFGSKGAIHITNDTGSTAVFSGENGVVAEKPKY
                     FFLERYMQSFADEISCFVDSVVNDKPTLVNGNDGLQPVLIALAAKKSLEENRPVKLSE
                     VA"
BASE COUNT         1252 a          924 c         1038 g         1063 t
ORIGIN      
        1 taacttcgca gaaatgagaa ctggatcaca tctttttttc gatttccttg ctattataaa
       61 tgaaataaat ttttcatttg taaaaatcag tagaaaaccg accgcttgca aaagcgatgt
      121 aagccagagg aggctttatg cagactactc gtttaacggt tgcacaggcg ttagtgcaat
      181 ttttggataa tcaatattta gacgtggacg gcaaagagat taaattcgtt cacggtgttg
      241 ccgctatttt cggccacggc aatgtgctgg gaataggtca ggcgcttgct caaaatgccg
      301 gtggtttaac gctctaccaa ggcagaaatg agcaaggtat ggcacatttg gcgatgggct
      361 ttgctaagca aaatctgcgt caaagaatta ttgcctgtac ttcctcggtc gggccggggg
      421 cggcaaatat ggttacggct gcggcaacgg caacagcgaa ccgtattcct cttcttttat
      481 taccgggcga tgtgtttgcg acccgccagc cggatccggt actgcaacaa atcgaacaac
      541 cttacgattt aagcattagc actaacgatg cgttccgtgc cgtcagtaaa tattgggata
      601 gagtgcaacg ccctgagcag ttaatgagtg cttgtatcaa tgcgtttcgt gtgctgaccg
      661 accctgctga aaccggtgct gtcaccattg ctctgccgca agatgtacaa gcggagagtt
      721 ttgatttccc gaacagcttc ctgcaaaaac gagtgcatcg cttagagcgt acaccgccga
      781 caacagcgat gattaagcaa gcggtaacat taattcaaac caagaaaaaa ccgctgattg
      841 tctgcggtgg cggtgttcgc tactcgcaag cgggtcaaat tctgcaaaaa tttgctaaac
      901 agttcggcat tccatttgct gaaacccaag cgggtaaaag tgcggtggtg tccgatcacg
      961 cattgaatgt aggcggagtg ggagaaaccg gttgtttagc ggcaaaccgt ctggcaaaag
     1021 aagcggattt agtgattggc attggtacac gctacaccga cttcacgact tcttccaaat
     1081 ggattttcca aaacccaaac gtgcagtttt tgaacattaa tgtggcacgt tttgatgcgt
     1141 acaaattaga cggcgtgcaa ctggttgccg atgcaaaaac tgcattagag gcgttaagcc
     1201 aagccattcc tactgattat caagctcaat gggagaatga aatttcacag gcgaaagcgg
     1261 attttgctga tgaaattcaa cgcctgcgcg ctgtgcaata tcaccctgat ttcacccctg
     1321 aaattaacga taatcttgat cataaagcgg ttcacaatga atttattcag ctcaccggtt
     1381 cccagctcac ccaaacccaa gtgctgggta ttgtgaatga aaccattgat aacaatgcga
     1441 ttattgttgc cgccgccgga agtttgccgg gtgatttaca acgcacttgg ttaagtaaag
     1501 gtgaagagac ttaccacctt gaatatggct actcctgtat gggctatgaa gtgagtgcgg
     1561 cagtcggcgt gaaaattgcc cgccccgaac aagaggtcta tgccttgctg ggggacggct
     1621 cttacttaat ggcacacacc gaattagtga cctcaattca agagggcaaa aaaatcaacg
     1681 tggtgctgtt cgacaatatg gcaaacggct gtatcaacaa cctgcaaatc ggcaacggta
     1741 tggacagttt cggtacggaa ttccgcttcc gcaatggcga aactaaccaa ctcaacggcg
     1801 gtttagtgcc gattgatttt gccaaaaacg ccgaagcata cggctgcaaa acctatcggg
     1861 taactaacga agccgaattg cgtgccgccc ttgatgatgc gaaaaaacag accgtttcta
     1921 ccttaatcga tattaaagtg ttgccgaaaa caatggctca cggctatggc tgctggtggc
     1981 acgttggtgt cgcggaagtg tcagagagcg agcgtattca acaagcccgc caacgctccg
     2041 aagagcagtt agctaaggct cggaagtatt agttaaaaaa acaactcccc actttgagct
     2101 acgaggggag aatatcaagc ggtcaaattt gcaaaatttt tgataaaaat aaccgcttgc
     2161 atccaaattt atctctataa atcaaacgag gtttctctat gaaagctgaa aacattcaat
     2221 taggcattgc cccaatcggt tggacgaacg atgacctgcc ggaactggga gcggaaaaca
     2281 ctttcgagca gtgtgtgagt gagatggcac ttgccggcta cacgggctgt gaagtgggta
     2341 ataaatatcc gcgtgatgtt gccgtgttaa aacacaaact ggacgtgcgt ggcattcaaa
     2401 tctgtaatgc gtggttcagc accttctttg tggacggtaa acgggaagag accattgccg
     2461 agttcatcaa acatcgtgat ttcttgcatg caatgggggc aaaagtgatc ggctgttccg
     2521 aacagagccg tagtattcaa ggcacaacca aatctgtgtt caaagaaaaa ccgattttca
     2581 acgatgaaga ttggaaacgt atggcagaag gctataacga attggcgaaa cttgccgccg
     2641 aaaaaggaat gaaagtctgc ctgcatcacc atatgggaac cggtattcaa acacccgaag
     2701 aggtcgatcg ctatatggca gaagtgaatg acgatgtcta tctgttgttc gattcagggc
     2761 atttgtacta ttccgaaggc tgccaaaaag cgatgctcgg cgtactggaa aaatatatcg
     2821 accgtattat ccacgttcac ttaaaagatg tgcgtgatga agtcatggcg gaagtaaaag
     2881 caaaagattt aagcttttta gaaggcgtga aaaaaggcac ctttactgta ccgggcgatg
     2941 gtgtcattga tttccgtccg attttcgata ttttagaaaa acacgactac aaaggctgga
     3001 tggttgtaga agccgaacaa gatccggcac ttgccaaccc gtttgaatac gccctcaaag
     3061 gcagaaaata tatccgtgaa accgcaggag tttaatatgt taaaagtagg tattattggt
     3121 gcaggccgta ttggacgtgt gcattctgaa agtattagca aatatgtcaa aggtgctgaa
     3181 atcaaagcaa tctctgatgt aaaaattacc gaagaattga ccgcttgggc aaaatcgatg
     3241 ggtattccgg aagtttatga tgactataaa aaaatcctgc aagatccgga aattgatgct
     3301 gtattggtct gctcttccac caacactcac gctccgattt cgattgaggc agcacaggct
     3361 ggcaaacacg ttttctgtga aaaaccggta gatgcagacc cagctaaaat ccgtgaagta
     3421 ttgagtgcag tcgaaaaagc cggtgtaaaa ttccaagtgg gctttaaccg ccgcttcgac
     3481 cacaacttca aggcgattaa agaccgtgtg gcggcaggcg acatcggcga accgcacgtt
     3541 atccgagtaa cctctcgtga tccggaagcc ccgccaatcg aatatgtgaa agtctccggc
     3601 ggaatgttct ttgatatgac cattcacgac ttcgatatga ttcgttattt atccggcagc
     3661 gaagtggaag aagtgtttgc agtcggcacc gtattagtaa accctgaaat cggcaaagcg
     3721 ggcgatattg atactgccgt tatcacgttg aaactgaaaa acggagcaat cggcgtaatc
     3781 gacaacagtc gcaaagctgc ctacggctac gaccaacgtg cggaagtgtt cggctcgaaa
     3841 ggggcaatcc atatcactaa tgataccggc tcaactgccg tattctcagg tgaaaacggc
     3901 gtggtggcgg aaaaaccaaa atacttcttc ttagaacgtt atatgcaatc tttcgccgat
     3961 gaaatcagct gtttcgtaga ttctgtggta aacgacaaac caacgttagt aaacggcaat
     4021 gacggcttac aaccggtact tattgcctta gctgcgaaaa aatcactgga agaaaaccga
     4081 ccagtgaaat tgagtgaagt ggcgtaattg atcataaata caagcggtcg gttttactcg
     4141 ctacactcgc acttcgtgcc atcggcatag ccgatgttca aacgcaagca ttttgcagaa
     4201 aaatttgtaa aactgaccgt ttgagttttt atttattatc ttttgggaaa taaatataat
     4261 cgtgctcaat ttttatt
//
LOCUS       DANLXS010000193         4270 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.196, whole genome shotgun sequence.
ACCESSION   DANLXS010000193 DANLXS010000000
VERSION     DANLXS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.196"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            37..1245
                     /locus_tag="PXD71_002315"
     CDS             37..1245
                     /locus_tag="PXD71_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL6292384.1"
                     /translation="MSKNLILILNCGSSSLKFAILDPKTGDEKLSGLAEAFHLEDARI
                     KWKLNGEKGNADLGAGAAHSEALTFIAEKLLSDELKAGIGAIGHRIVHGGEKFTSSVV
                     ITDEVIKGIEDATQFAPLHNPAHLIGIKEAFRIFPELKDKNVAVFDTAFHQTMPEHAF
                     LYALPYTFYKENGIRRYGFHGTSHFYITSQVAELAGKSVEQTNAIICHLGNGGSVSVV
                     RNGKCIDTSMGLTPLEGLVMGTRSGDLDPAIISFLYNNLGMSMDEIDTTLVKKSGLLG
                     LTGVTSDCRYAEDNYNDVSKPEAKRALDVYSYRLAKYIGSYMAILGDDHLDAIAFTGG
                     IGENSGLVRELTLNHLKLFGIKLDQERNLAARFGKDGVITADESTFKAVVIPTNEELV
                     IAQDTAKLAL"
     gene            1298..3433
                     /gene="pta"
                     /locus_tag="PXD71_002316"
     CDS             1298..3433
                     /gene="pta"
                     /locus_tag="PXD71_002316"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL6292385.1"
                     /translation="MSRTIILIPISTGVGLTSVSLGLVHALEQKGSKVGFLKPISQPI
                     SGEDTLDRSTTIIRAEQTTEVGEPFMLSEAETLIGQNQTDVLLEKVVERHQKLSKNNE
                     IVIVEGLIPTRKNSYANSINYEMAQALDAEIVLVAAPASDTPAQLKERVEAAASQFGG
                     RNNPNLLGVIINKFNAPVDDSGRTRPDLTEIFDSFQHSNNNVSEVEHLFAKSPIKLLG
                     CISWKAELIATRAIDVAKHLGASIINEGEIKARRIRGVTFCARNLPNMVDHFKAGSLL
                     VTSADRPEVLVAASLAVMNGIEIGAILLTGGYKIDSSIAKLCQQAFETGVPVFRIEGN
                     TWQTALSLQSFSLEVPVDDKERIAAIKEYMASKFNVGFIDEVSKAATRARRLSPAAFR
                     YQLTEYARQAKKRIVLPEGDEPRTVKAAALCAERGIAECVLLANPTDVQRVAEAQGVV
                     LGKGVTIIDPASIRENYVDRLVELRKAKGMTEVVAREQLLDTVVLGTMMLEANEVDGL
                     VSGAVHTTANTIRPPMQIIKTAPGSSIVSSIFFMLLPDQVLVYGDCAVNPDPTAEQLA
                     EIAIQSAESAKAFGIDPRVAMISYSTGTSGSGADVEKVKEATRIAQEKRPDLIIDGPL
                     QYDAAIMEDVARSKAPNSPVAGKATVFVFPDLNTGNTTYKAVQRSADLVSIGPMLQGM
                     RKPVNDLSRGALVDDIVYTIALTAIQATQ"
     gene            3547..4227
                     /gene="can"
                     /locus_tag="PXD71_002317"
     CDS             3547..4227
                     /gene="can"
                     /locus_tag="PXD71_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDL6292386.1"
                     /translation="MEKIERLFANNHAWATKMKDEQSDYFKELAEHQKPTYLWIGCSD
                     SRVPAEKLTGLGPGELFVHRNVANMVIHTDLNCLSVVQYAVDVLEIEHIIICGHTNCG
                     GINAAIAQKELGLINNWLLHIRDIWFKHSHLLGKLPYEERSNVLTRLNVAEQVYNLGQ
                     SSIIKTAWEQGKSLSLHGWVYDVTDGFLIDQGVMATSRETLEITYRNAIAKITTEANE
                     KIVLNDEN"
BASE COUNT         1333 a          793 c          870 g         1274 t
ORIGIN      
        1 cttcaaactc atttcataaa ataaggtaat ttgaccatgt ctaaaaactt aatcttaatc
       61 cttaactgcg gtagttcatc tcttaaattt gctattttag atcctaaaac aggtgatgaa
      121 aaattatctg gacttgctga agcttttcat ttagaagatg cacgtatcaa gtggaagtta
      181 aacggggaga aaggtaatgc tgatttaggt gcaggtgccg cacatagtga agcacttacc
      241 tttattgctg aaaaattgtt atctgacgaa ttaaaagcag gtatcggcgc gattggtcat
      301 cgtattgttc atggcggtga aaaattcacc tcatcagttg taattaccga tgaagtgatt
      361 aaaggtattg aagatgcaac gcaatttgca ccattacaca accctgcgca tttaattggt
      421 attaaagaag cattccgcat tttccctgaa ttaaaagaca aaaacgtagc tgtatttgac
      481 actgcattcc atcagacaat gccggaacac gcattcttat atgcacttcc atatacattc
      541 tataaagaaa atggtattcg tcgctacggt ttccatggca caagccactt ctatatcacc
      601 tcacaagttg cagaattagc aggtaagtct gttgagcaaa ccaatgccat tatttgccac
      661 ttaggtaacg gtggttctgt ttctgttgta cgcaatggta aatgtattga tacctcaatg
      721 ggtttaactc cattagaagg tttagtaatg ggtactcgct ctggtgactt agaccctgcg
      781 attatctctt tcttatacaa taacttaggt atgtcaatgg acgaaattga tactactcta
      841 gtaaaaaaat ctggtctttt aggtttaact ggcgtaacta gtgactgtcg ttatgcggaa
      901 gataattaca atgacgtatc aaaaccggaa gcaaaacgtg ctttagatgt atatagctat
      961 cgtttagcta aatatattgg ttcatatatg gcaattttag gcgatgacca cttagatgct
     1021 atcgcattta caggtggtat cggtgaaaat tcaggcttag tacgtgaatt aaccttaaat
     1081 cacttaaaat tatttggcat taagttagat caagagcgta accttgctgc ccgttttggt
     1141 aaagatggag tcattacagc ggacgagtca acatttaaag ctgttgttat cccaactaat
     1201 gaagaactcg tgattgcaca agataccgct aaattagcat tataatcata gggggcagat
     1261 attctgccct ttcactttct tttaatttgg aaatattatg tctagaacga tcattcttat
     1321 ccctatttca actggtgttg gtttaaccag tgttagctta ggtttagtac atgctttgga
     1381 gcaaaaaggt tctaaagtag gatttttaaa accgatttct caacctatca gcggtgaaga
     1441 tacgttagac cgctcaacaa cgattatcag agcagaacaa acaacagagg ttggtgagcc
     1501 gtttatgtta agcgaagctg aaacattaat cggtcaaaac caaacggatg tattgttaga
     1561 aaaagtggtg gaacgccacc aaaaattatc aaaaaataat gaaattgtca ttgttgaagg
     1621 tttaattcca acgcgtaaaa actcatacgc taacagcatt aactatgaaa tggctcaagc
     1681 ccttgatgct gaaattgtat tagttgcagc accggcctca gatacaccag ctcaattaaa
     1741 agagcgagtt gaagctgccg cttcacaatt tggtggacgt aacaacccaa atttactggg
     1801 cgttattatc aataaattca atgcaccagt agatgattca ggtagaaccc gtccagattt
     1861 gactgaaatt tttgattcat ttcaacactc aaataataat gtttcagaag tcgagcatct
     1921 ttttgctaaa agtccaatta agctgttagg ctgtattagc tggaaagcag aacttattgc
     1981 tacacgagca attgatgttg caaaacattt aggcgcttca attattaatg aaggcgaaat
     2041 aaaggctcgt cggattcgcg gtgtgacatt ctgtgcacgt aacttaccaa atatggtcga
     2101 tcattttaaa gccggtagtt tattagttac ctctgcggat cgtcctgaag tgttagttgc
     2161 tgcatcgttg gcggtaatga atggcatcga aattggtgct attttactaa ccggtggtta
     2221 taaaattgat agctcaattg caaaactttg ccaacaagca tttgaaaccg gtgtgcctgt
     2281 attccgtatt gaaggtaaca cttggcaaac cgcattaagc ctacaaagct tcagcttaga
     2341 agtgcctgta gatgataaag agcgtattgc tgcaattaaa gaatatatgg caagcaaatt
     2401 taatgtaggt ttcattgatg aggtttctaa agccgctact cgtgcaagac gtttatcacc
     2461 tgcagcattc cgttaccaat taaccgaata tgcccgtcaa gcgaaaaaac gcattgtttt
     2521 acctgaagga gatgagcctc gtactgtgaa agcggctgca ttatgtgctg agcgtggcat
     2581 tgcagaatgt gtgttattag ctaacccaac agacgtacaa cgtgttgctg aagcacaagg
     2641 cgtggtatta ggtaaaggcg taacgattat tgaccctgcg agcattcgtg aaaattatgt
     2701 tgatcgctta gttgaattgc gtaaagcaaa aggaatgact gaagttgttg ctcgtgagca
     2761 acttttagat acagttgtat tgggtacaat gatgcttgaa gctaatgaag tggacggttt
     2821 agtctcaggc gcagttcaca caaccgctaa cactattcgc ccaccaatgc agatcattaa
     2881 aactgcaccg ggaagctcta ttgtatcttc tatcttcttt atgctactac ctgatcaagt
     2941 attggtatat ggtgactgtg cggtaaaccc tgacccaact gcagaacagc tagcggaaat
     3001 tgcgattcaa tcagcagaat ctgccaaagc ctttggtatt gaccctcgtg ttgcaatgat
     3061 ctcctactct accggtacat caggttcagg tgcggatgta gaaaaagtga aagaagcaac
     3121 tcgtattgcc caagaaaaac gtccagattt aatcattgat ggcccattac aatatgatgc
     3181 ggcgattatg gaagatgttg cccgctcaaa agcaccaaat tcacctgtag caggtaaagc
     3241 aactgtattt gtcttcccgg atttaaatac tggtaatacc acctataaag cggtacaacg
     3301 ttcggcagat ttagtctcta tcggcccaat gttacaaggt atgcgtaaac ctgtaaatga
     3361 cttgtcccgt ggggctttag ttgacgacat cgtttatact attgcgttaa ctgcgattca
     3421 agcaacgcaa taattccctc ccaaatgaaa ccaaatgccc ttaaaactaa gggcattttt
     3481 attttttacc tacccgactt ttcaatttaa tcatataatc aagattagtg ttaaaaagga
     3541 gaatttatgg aaaaaattga acgcttattt gccaataacc acgcctgggc aactaagatg
     3601 aaagatgaac aatcggatta ttttaaagaa ttagcggaac atcaaaagcc aacctattta
     3661 tggattggct gttccgatag tcgtgtgcct gctgaaaaac tcacagggtt aggccccgga
     3721 gaattatttg ttcatcgaaa tgtagcaaat atggtgattc acactgatct taattgtctg
     3781 tcggttgttc aatatgcggt agatgtatta gaaattgagc atattattat ctgtggtcac
     3841 actaattgtg gtggtatcaa tgcggcaatt gcacaaaaag aacttggttt aattaataac
     3901 tggttgttac atattcgtga tatctggttt aaacacagcc atttactagg caaacttccc
     3961 tatgaagagc gatcaaatgt cttgacccga ttaaatgttg cagaacaagt ttataattta
     4021 ggtcagtcct ctattattaa aaccgcttgg gaacagggta aatccctctc gctacacggt
     4081 tgggtatacg atgttacaga tggtttcttg attgaccaag gtgttatggc tactagccga
     4141 gaaacattag aaattaccta tcgtaatgca atagctaaaa taacaaccga agcaaacgaa
     4201 aaaatagttt taaacgatga aaattagcca ttgtaagcgg ttaaatttat cgattttttt
     4261 acaatctttt
//
LOCUS       DANLXS010000194         4259 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.197, whole genome shotgun sequence.
ACCESSION   DANLXS010000194 DANLXS010000000
VERSION     DANLXS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.197"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(47..122)
                     /locus_tag="PXD71_002318"
     tRNA            complement(47..122)
                     /locus_tag="PXD71_002318"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Asn,seq:gtt)
     gene            complement(238..987)
                     /gene="moeB"
                     /locus_tag="PXD71_002319"
     CDS             complement(238..987)
                     /gene="moeB"
                     /locus_tag="PXD71_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDL6292387.1"
                     /translation="MELTDHEMLRYNRQIILKSVDFDGQEKLKASRVLIVGLGGLGCA
                     ASQYLASAGVGHLTLVDFDLVSLSNLQRQILHTDATIGQPKVESAKARLAAINPNIQI
                     ETINQRCSDEQLAKLIANVDLVLDCTDNVTVRNQLNLQCFKQKRPLVSGSAIRFEGQV
                     SVFCYQEQEPCYECLSRLFGDATLSCVEAGVIAPIVGIIGSIQALEAIKVLLNIGRTL
                     SGKLLMIDGLHFSVREMALPKQNNCPVCTSY"
     gene            complement(1086..2315)
                     /gene="moeA"
                     /locus_tag="PXD71_002320"
     CDS             complement(1086..2315)
                     /gene="moeA"
                     /locus_tag="PXD71_002320"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDL6292388.1"
                     /translation="MALLALSDALDNMLARLSTPKLVEQLPLNECANRVLAEDIFSPI
                     NVPNFDNSAMDGYAVRISDLAQNLTLNVAGKAFAGNPFNGEIKAGECVRIMTGALVPK
                     GVDAVVMQEETTLNSDGSVTFNHIPKLGSNIRRIGEDVKQGALVLKKGSLLNVASLPL
                     LASLGIASVPVFVKLKVAILSTGDELTSVGEPLSEGKIYDTNRFAVRLMLEKLNCEIL
                     DFGILPDDPILFEQTFRQAQQQADVLITSGGVSVGEADFTKDVLEKLGEIGFWKIAMK
                     PGKPFAFGKLEQAWFFGLPGNPVSALVTFYQLAQPALAKLSGLSTDKIAKLTQNLTAC
                     SAGKLKKAVGRQDFQRGYYYANEKGELEVRAVGTQGSHIFSAFNESNCFIVLEAERGN
                     VDAGEKVTIQPFNSLMY"
     gene            complement(2337..2954)
                     /locus_tag="PXD71_002321"
     CDS             complement(2337..2954)
                     /locus_tag="PXD71_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HDL6292389.1"
                     /translation="MFDSLAVQFVVLWAVIDPIGSVPVYLAKTIGLPIEARRKIALNA
                     VLIAAGILIFFLIGGQVLLESMQIPLPAFQAAGGLVLLLFALTMIFGESKPEQEMRIS
                     SNLNELAVYPLAVPSIASPGAMMAIVLLTDNHRFHIGDQIITAGIMLLVLFITYLLLL
                     AANRIQHLIGNAGAAIISRVMGLILAAIAVNNLLIGVRDFFVQIS"
     gene            complement(3022..4233)
                     /locus_tag="PXD71_002322"
     CDS             complement(3022..4233)
                     /locus_tag="PXD71_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL6292390.1"
                     /translation="MISRQMQSLLKRLVAQFPAVLVTGARQVGKSTLLQHIGEGYQYI
                     TFDDPMLLELAKQESRLFFLNHQGKLILDEVQYVPEIFALLKLEIDKKQQNGLFLLSG
                     SQAFELMQNVSETLAGRIAILKLQGLSLREILGVKFDQPFLPTTDYLLAREQELKSPE
                     NIWQLIHKGDMPRLYAQEVDWEIYYRSYVATYIERDVRQLTNIASTTDFTRFMVSIAS
                     RSGELLNYSNIAQEVGVSNETIKRWVAILQTSGIIYLLQPYHNNHLKRAIKTPKVYFL
                     DTGLMAYLTKWLTPETIAQGAKSWQFFETFVVSQIIKSFHNQGIEPPIYFYRDTNQKE
                     IDLIIEYDRTLYPIEIKVTASPTKKMAKSFDLLNSVSSEWKLGQGAIINQYPTKLYLS
                     ENLVALPVSYL"
BASE COUNT         1302 a          992 c          784 g         1181 t
ORIGIN      
        1 tataaaagga gacaaataaa acaagaacaa aagaaaaata aaatcttggc tcctcctgcg
       61 ggactcgaac ctgcgacata tggattaaca gtccaccgtt ctaccgactg aactaaggag
      121 gaattaacgg tgtaaataat aggctaaaac gttttagccg tcaaataaaa aaaataaact
      181 gtcgttcatt ttttacattt cttgacattt tttcgccaat aagtatgaga aaaatactta
      241 ataagaggta caaaccggac agttattttg cttaggtaat gccatttctc tgacagaaaa
      301 atgcaagccg tcaatcatta acaattttcc ggaaagagtt ctgccgatat taagtagcac
      361 tttaattgct tccaacgctt gaatactgcc tatgatgcca acaatcggag caatgacccc
      421 cgcttcaaca caacttaaag tcgcatcacc aaacaacctg cttaaacatt catagcacgg
      481 ttcttgttct tgataacaaa atacactcac ctgcccttca aatcgaattg ccgaaccgga
      541 aacaagcggt cgtttttgct taaaacattg caaatttaac tgattacgca cagtaacgtt
      601 atcggtacaa tccagcacta aatctacatt tgcaatcagc ttggctaact gctcatcgga
      661 acatcgctga ttgatagttt caatctgaat gttcggatta atcgccgcta atcgagcttt
      721 ggcagattcc acctttggct gcccgatagt cgcatcggta tgtaaaattt ggcgttgcag
      781 attagaaagt gagaccagat caaaatccac caaagtcaaa tgcccaacgc cggcagaagc
      841 taagtattgc gatgccgcac agcccaaacc acctaaaccg acaatcaaca ctcggctggc
      901 ttttaacttt tcctgcccgt caaaatccac gctttttagg ataatctggc gattataacg
      961 tagcatttcg tgatcggtaa gttccatttt tacacctaaa gagtaaacga tggcgcaagc
     1021 gtccacgctt gtgcctatat ttttattgct agcacaagcg tggacgcttg cgctatctta
     1081 attcattaat acatcagcga attaaacggc tgaatagtga ctttttcccc agcatcaaca
     1141 ttgcctcgtt ctgcttctag cacaataaaa cagttacttt cattaaaagc gctgaaaata
     1201 tgtgagcctt gcgtgccaac tgcacgaact tctaactcac ctttttcatt ggcatagtaa
     1261 tagccccgtt ggaaatcttg gcgaccgacc gcttttttca attttccggc actgcaagcg
     1321 gttagatttt gggttaattt tgcaatttta tctgtcgata aacccgaaag tttggctaac
     1381 gccggttgcg ctaattggta gaaagtcact aatgcagaga cagggttgcc cggcagccca
     1441 aaaaaccaag cctgttctaa tttaccgaat gcaaaaggct tgcccggctt catcgcaatt
     1501 ttccagaaac cgatttcgcc taatttttcc agtacatctt tggtaaaatc tgcctctccc
     1561 acagatacgc cgccactggt aatcaacaca tcggcttgct gttgggcttg cctgaaagtt
     1621 tgctcaaaca gaattgggtc atcaggcaaa atcccaaaat ctaaaatctc gcagttgagt
     1681 ttttccagca ttaaacgcac cgcaaaacgg ttggtgtcgt aaatttttcc ctcactcaaa
     1741 ggctcgccaa ctgaggttaa ttcatcgccg gttgaaagaa ttgccacttt taattttaca
     1801 aaaacaggca cagaagcaat accgagtgaa gctagcaacg gtaaactggc tacattcaat
     1861 aatgagcctt ttttcaagac caatgccccc tgtttaacat cttccccaat gcggcgaatg
     1921 ttactgccta attttggaat atggttaaag gttacagagc catcggaatt taacgtagtt
     1981 tcctcttgca ttacaacggc atcaacgccc tttggcacta atgctcccgt cataatccgc
     2041 acgcattcgc ctgcctttat ttcgccgtta aacgggttac cggcaaaggc tttaccagcc
     2101 acattcaagg ttaaattttg tgctaaatct gaaatacgaa ccgcgtagcc gtccatcgcc
     2161 gaattatcaa aattcggtac attaatcggg gaaaaaatat cctctgccaa cactcggtta
     2221 gcacactcat ttaacggaag ttgttccacc aatttaggtg tcgataaacg agcaagcata
     2281 ttatctaacg catcactcaa agccaaaagt gccataattt cctacctatt caacgtttag
     2341 ctaatttgta caaagaaatc ccgtacgcca atcaacagat tattcaccgc aatcgcagcc
     2401 agaattaacc ccatcactcg gctgataatc gcagcccctg cattaccaat aaggtgctga
     2461 atacggtttg ccgctaatag caacaaatag gtaataaaga gtaccaaaag cataatgccg
     2521 gcggtgataa tttgatcgcc gatatgaaaa cggtgattgt cggttaataa tacgattgcc
     2581 atcatcgccc cgggagaagc aatcgacggt acagctagcg gataaaccgc caattcattc
     2641 agattactgc tgatccgcat ttcttgctcg ggcttgcttt cgccaaaaat catcgttaaa
     2701 gcaaataaca acagcactaa gccgcctgcc gcttggaatg caggtaacgg aatctgcatt
     2761 gattctaaca gcacttgccc accgataagg aagaaaatca aaataccggc ggcaatcaag
     2821 accgcattta gtgcaatttt acgccgtgct tcaatcggca aaccaatggt ttttgccaaa
     2881 taaacaggaa cagaaccaat cgggtcgatc accgcccata aaaccacaaa ttgtaccgct
     2941 aatgaatcaa acatattctc tcctgttatc ttgataattt gcaaaatttt agcataaaac
     3001 aaccgcttgc acggcttttt cttacagata gctcaccggt aacgctacca aattttcact
     3061 taaataaagt ttggtcggat attggttaat aatcgctccc tgccctaatt tccattcact
     3121 agaaactgag tttagcaagt caaaactttt tgccattttt ttggttgggc ttgccgtgac
     3181 cttaatttct atcggataaa gcgtgcgatc atattcaata attaagtcga tctctttctg
     3241 gttagtatca cggtaaaaat aaatcggcgg ctcaattcct tgattatgaa aggattttat
     3301 aatttggctt accacaaagg tttcaaagaa ctgccagctt tttgcccctt gagcaatagt
     3361 ttcgggggta agccatttgg ttaaatacgc cattaacccg gtgtctagaa aatacacttt
     3421 tggggtctta attgcccgtt tcaaatgatt gttgtggtat ggctggagca aatagataat
     3481 gccggaagtc tgcaaaatgg cgacccaacg tttaatcgtt tcattcgata ctccaacttc
     3541 ttgagcaata ttactgtaat tgagtagctc accactacga gaggcaatag agaccataaa
     3601 acgggtaaaa tccgttgtgc tggcaatatt ggttaattgg cgaacatctc gctcaatgta
     3661 ggtcgctaca taagagcgat aataaatctc ccaatccacc tcttgtgcat ataaacgagg
     3721 catatcgcct ttatggataa gctgccagat gttctccggt gattttaatt cttgctcccg
     3781 agctaacaaa tagtcggttg tcggtaaaaa aggttgatcg aacttcaccc ctaaaatttc
     3841 acgcaacgaa agcccttgta attttaaaat agcaatacga ccggcaagtg tttcactcac
     3901 attctgcatt aattcaaagg cttgcgaccc cgaaagtaaa aacaaaccgt tttgctgttt
     3961 tttatcaatt tctaacttta ataacgcaaa aatttcaggc acatactgta cttcgtctaa
     4021 aataagcttg ccttgatggt ttaaaaaaaa caggcgagat tcttgtttag ccagctccag
     4081 caacattggg tcatcaaaag taatatattg atagccctca ccaatatgtt gtaacaatgt
     4141 cgatttacca acctgtcttg cccctgtcac taaaacggca ggaaattgcg ccacgagtcg
     4201 ttttaacagc gattgcattt gccgagaaat catttttcct ccaaaatagc ctaaatcaa
//
LOCUS       DANLXS010000195         4226 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.198, whole genome shotgun sequence.
ACCESSION   DANLXS010000195 DANLXS010000000
VERSION     DANLXS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.198"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(3..1409)
                     /locus_tag="PXD71_002323"
     CDS             complement(3..1409)
                     /locus_tag="PXD71_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDL6292391.1"
                     /translation="MKLTKIVLSVVAVIGAVAVGGSWYTGKQVEEKYQELVVQANNQL
                     KQLSGEFGANIELKDVQIDRHFFISNAKYRLEVDLGNGKKLDFVGNDKLHHGPLPLNR
                     LAKLNLAPVMMSAESKLQAPEALKKLMGEHIGTGFSNISYSGDVEGHFDISPIKHSDE
                     TGHIETTPIKVQYSYDQKLKGVDSTVELESLKIKAEEFDFQIQGIVYDVQTENNESYP
                     NLTLGKGSGKINAIEFKSKDGKLSQIKDVVVKGDNLLKGDRLISTGDLEAATFNIEGV
                     EMGKLKMDMAYDLDAKLTNDITPLLSNPQTLENEKTGELLLQLLAKSFKFHINNFSLE
                     NSKGKVDLALLLNMAQFDPQNLGNMQAVLQALSTSKFTSNINRQYAEDIIRQVSIVTE
                     KLGEEEAKAFAKQQVDAVFLNAGVEQYGLRKVDDNNVKIELTIDNGKVNLNGRELTED
                     ELQMALFMIVMGAGSLGQ"
     gene            complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXD71_002324"
     CDS             complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXD71_002324"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDL6292392.1"
                     /translation="MSRFDTLFTALKAKNEGAFVPFVTLCDPGFERSFEIIDTMIKNG
                     ADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDSCFELIAKIRAKYPDIPIGLLLC
                     ANLIFVPTAEVFFQRCQQSGVDAVLIADLPVFAAQEFVPTAEKYGIQPVFICPPNADQ
                     NTIERIAQLTQGYTYLVSRAGVTSAENQAHASNLDNLVESLKQAGAAPILQGFGIAKP
                     EQVKEALRLGADGAISGSAIVKIIERNLADQANLLKELAEFVQVMKAATK"
     gene            complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXD71_002325"
     CDS             complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXD71_002325"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDL6292393.1"
                     /translation="MSDTLLNPYFGEFGGMYVPEILVPVLKELEQAFVDAQQDPTFQA
                     EFADLLKNYAGRPTALTLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRM
                     GKTRIIAETGAGQHGVATALACAMLGMPCVVYMGAKDVERQSPNVFRMRLMGAEVIPV
                     QKGSCSLKDACCEAMRDWAANYDTTHYLIGTAAGPHPFPTMVREFQKMIGEEIKRQIL
                     AKEDRLPDAVIAAVGGGSNAIGMFADFIEEQSVKLIGVEPAGKGIETGEHGAPLKHGT
                     TGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNATGRAEYVSITDAEAL
                     EAFQALAKNEGIIPALESSHALAYALKMIKQNPEKEQLLVVNLSGRGDKDIFTVDKIL
                     NGGE"
     gene            complement(3469..4221)
                     /locus_tag="PXD71_002326"
     CDS             complement(3469..4221)
                     /locus_tag="PXD71_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6292394.1"
                     /translation="MKIALVTGVTAGFGLAICKLLLQSGYKVIGTGRRAERLEELHLQ
                     FGDKFLPLAFDIQQPDEVEKALNSRPKAWQQIDLLVNNAGLALGLEPAQKADLNDWYQ
                     MIDTNIKGLVTMTRLVLPEMVERKSGHIINIGSIAGTYPYPGGNVYGGTKAFVKQFSL
                     NLRADLAGTKVRVSNVEPGLCGGTEFSNVRFKGDDEKAAKVYENVQFVRPEDVANIVL
                     WLNQQPEHVNINRVEVMPVAQTSSALNVARNI"
BASE COUNT         1161 a          985 c          754 g         1326 t
ORIGIN      
        1 gattattgcc cgagtgagcc tgctcccatc acaatcataa acagagccat ttgaagttca
       61 tcttcggtca gttcacgtcc gtttaaattc acttttccgt tatctatagt taattcaatc
      121 ttcacattgt tgtcatctac ttttcttaat ccgtactgtt caactcccgc attgagaaaa
      181 acagcatcaa cttgttgctt ggcaaatgct ttcgcctctt cttcgcctaa tttttctgta
      241 actattgaga cttggcgaat aatatcttcc gcatattgac ggttgatatt tgaagtgaat
      301 ttacttgttg aaagggcttg caataccgct tgcatattac caagattttg tgggtcaaat
      361 tgagccatat tcagtaataa agccaaatca actttgcctt tgctgttttc aagtgagaaa
      421 ttattaatat ggaatttaaa tgattttgct aatagttgta ataataactc gcctgttttt
      481 tcattttcta atgtttgagg attagataat aaaggcgtaa tatcattggt taatttagca
      541 tctaaatcgt atgccatatc catttttaat ttacccattt caacaccctc aatgttgaaa
      601 gtggctgcct ctaaatctcc ggtagaaatt aaacgatcgc cttttaatag attatcgccc
      661 ttcaccacaa catctttaat ttgagataat ttgccatctt tagacttaaa ctcaatagcg
      721 ttaatttttc cactaccttt gcctaaagtt aaattaggat aactttcatt attttctgtt
      781 tgaacatcat aaactatacc ttgaatttga aaatcaaatt cttctgcttt tatcttaaga
      841 ctttctagct caacagtgct atcaacccct tttaattttt ggtcgtagct atattgcact
      901 ttgattggcg tggtttcaat atgacctgtt tcatcactat gtttaatcgg ggaaatatca
      961 aaatgccctt ccacatcgcc cgaatagctg atattagaaa aaccggtgcc aatatgttca
     1021 cccatcaatt ttttaagggc ttcaggagct tgtaatttgc tttccgcact catcattaca
     1081 ggagctaaat taagtttcgc taaacgatta agcggtagcg gaccgtgatg aagtttatca
     1141 ttaccaacaa agtccaattt cttaccatta cccaaatcca cttctaaacg atatttcgca
     1201 ttagagataa agaaatggcg atcgatctgc acatctttaa gctcaatatt cgcaccaaat
     1261 tcgccactca gttgtttaag ttggttatta gcttgaacaa ccaattcttg atatttttct
     1321 tcaacctgct tacctgtgta ccaacttccg ccaactgcaa cagcaccaat taccgctaca
     1381 actgaaagaa caatttttgt taatttcatt ctgtttcctt ttattttcaa aaaaatttaa
     1441 aaaatgaccg cttgtagttt acttcgtagc cgctttcatc acttgtacaa attcggcaag
     1501 ttctttgagc aaattagctt ggtcagctaa attacgttca ataattttca caatcgctga
     1561 gcctgaaatc gccccgtctg ccccaagcct taacgcctct tttacctgtt caggtttggc
     1621 tatgccaaaa ccttgtaaga taggggcagc tccggcttgt tttaaacttt ccactaaatt
     1681 atccaaatta cttgcgtgag cttggttttc agcactagtt acaccggcac gggaaactaa
     1741 ataagtgtag ccttgggtta attgagcaat ccgttcaatg gtgttttgat cagcattcgg
     1801 cgggcaaata aacaccggct gaattccata tttttcagcg gttggcacaa actcttgagc
     1861 agcgaaaaca ggcaaatcag caatcaacac cgcatcaact ccactttgtt ggcaacgttg
     1921 gaaaaatact tctgcggttg gcacaaaaat taaattggca cacagtagta aaccgatagg
     1981 aatgtctgga tatttagctc gaattttggc aatcagctca aaacagctat cagtgctata
     2041 gccaccatct aaagctcgtt tattcgctgc ttgaatcacc ggcccgtcta agagggggtc
     2101 agaaaacgga aagcctagct ctaaagcatc agccccgttt ttaatcatcg tgtcgataat
     2161 ctcaaaactg cgttcaaaac caggatcgca taatgtcaca aacggcacaa aagccccttc
     2221 gttttttgct tttaatgcag taaatagcgt atcaaagcgg ctcattttac tctcctccat
     2281 ttaaaatttt atccacggtg aaaatgtctt tatcgcctcg acccgataaa ttcaccacta
     2341 ataactgctc tttttcaggg ttctgcttaa tcatttttaa cgcataggct aaggcgtggg
     2401 aagattctaa cgccggaata atcccctcat ttttggctaa tgcttggaat gcctctaaag
     2461 cctctgcatc ggtaatcgat acatattcag ctcgccctgt cgcatttaaa taagcgtgtt
     2521 gcggcccgac agaagggaaa tctaagccgg ctgaaatgga gtaagactct tcaatttgcc
     2581 cgtctttatc ctgcataatc ggcgatttca taccgaaata gatccccgtt gtgccgtgtt
     2641 tgagcggtgc accgtgttca ccggtttcaa ttccttttcc tgccggctca acaccgatca
     2701 atttgacgga ttgctcttcg ataaaatctg caaacatacc gatagcattt gagccaccgc
     2761 ctacggcagc aatcaccgca tcaggcaagc ggtcttcttt tgctaaaatt tgacgtttga
     2821 tctcttcgcc aatcattttc tgaaattcac gcaccattgt cgggaacggg tgcgggccgg
     2881 ctgcagtgcc gattaggtaa tgggtagtat cataatttgc cgcccaatcc cgcattgcct
     2941 cgcaacaggc atctttgagc gagcaagagc ctttttgtac cggaatcacc tccgctccca
     3001 ttaaacgcat acggaataca tttggcgatt ggcgttctac atcttttgcg cccatataca
     3061 ccacgcaagg cattcctaac atcgcacacg ctaatgcggt tgccacaccg tgctgtcctg
     3121 ctcctgtttc ggcaataatg cgggttttac ccattcgttt agcgagtaag atttgcccta
     3181 gcacttggtt ggttttatgt gccccaccgt gtaaaagatc ttcacgtttt aaatagagtt
     3241 tggttttact gcctttggtt aaattacgac aaagggttaa agcagtcggg cgaccggcat
     3301 agtttttgag taaatcggca aattcagctt ggaatgttgg gtcttgctga gcatctacaa
     3361 aggcttgttc taattctttt agcaccggca ctaaaatttc cggcacatac ataccaccaa
     3421 attcaccaaa ataaggatta agaagtgtgt ctgacataat atttcctttt aaatatttct
     3481 cgctacattt aaggcagaag aagtttgagc caccggcatc acttcaaccc gattaatatt
     3541 aacgtgttca ggttgctgat tcagccaaag cacaatattt gccacatctt ccgggcgcac
     3601 aaactgcacg ttttcataaa ctttagccgc tttttcatca tcgcctttaa agcgtacatt
     3661 tgaaaattcc gtaccgccac atagccccgg ctcaacattg gaaacacgca ctttagtgcc
     3721 ggctaaatct gcccgcaaat ttaggctaaa ctgtttcacg aaggctttag taccaccata
     3781 tacattaccg cccgggtaag ggtaagtgcc ggcaatcgag ccgatattaa taatatgccc
     3841 acttttccgc tccaccattt caggcaaaac taagcgtgtc atcgttacca atcctttaat
     3901 attggtatcg atcatttgat accaatcgtt caaatctgct ttctgtgccg gttctaagcc
     3961 gagtgcaagg cctgcattgt ttactaataa atcaatttgt tgccacgctt tagggcggct
     4021 attcagtgct ttttccacct catcgggctg ttgaatatca aatgcaagcg gtagaaattt
     4081 atcgccaaat tgtaaatgca gttcttctaa acgctctgcc cgtctgcccg tgccaattac
     4141 tttataaccc gattgaagca ataatttaca aattgccaac ccaaatccag cagttactcc
     4201 tgtgactaat gctattttca tctttt
//
LOCUS       DANLXS010000196         4183 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.199, whole genome shotgun sequence.
ACCESSION   DANLXS010000196 DANLXS010000000
VERSION     DANLXS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.199"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            93..1268
                     /locus_tag="PXD71_002327"
     CDS             93..1268
                     /locus_tag="PXD71_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL6292395.1"
                     /translation="MSVIKMTDLDLAGKRLFIRADLNVPVKDGKVTSDARIVATIPTL
                     KLALQKGAKVMVTSHLGRPTEGVFEEENSLQPVVDYLNASDLGVPVRLVRDYLDGVEV
                     NENEIVVLENVRINKGEKKNDPELAKKYASLCDVFVMDAFGTAHRAEGSTYGVAEYAP
                     VACAGPLLATELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGI
                     ANTFIAAEGHAVGKSLYEEDLIPEAKRLAQVTNIPVPVDVRVGTEFSETAPATEKAVS
                     EVRADESIFDIGEKSSEQLAEIIRNSKTILWNGPVGVFEFPNFRKGTEVISNAIAEAT
                     ANGAFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG"
     gene            1365..2441
                     /gene="fbaA"
                     /locus_tag="PXD71_002328"
     CDS             1365..2441
                     /gene="fbaA"
                     /locus_tag="PXD71_002328"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDL6292396.1"
                     /translation="MSLLNIVKPGVVTGDDVQKVFAYAKEHNFAIPAVNCVGSDSVNA
                     VLETAARVKSPVIVQFSNGGAQFYAGKGIKPASGARADVLGAIAGAKHVHQLAEEYGV
                     PVILHTDHAAKNLLPWIDGLLEAGEKHFAETGRPLFSSHMIDLSEEPIEENMEICREY
                     LARMDKIGMTLEIEIGVTGGEEDGVDNSDVDESKLYTQPSEVLYVYDQLSPISPRFTI
                     AAAFGNVHGVYKPGNVKLKPSILGASQEYVSKERGLPAKSLDFVFHGGSGSSREEIRE
                     AISYGAIKMNIDTDTQWAAWDGILQFYKANEAYLQGQLGNPTGPDAPNKKYYDPRVWL
                     RKMEESMSKRLEQSFEDLNCVNVL"
     gene            2542..3336
                     /locus_tag="PXD71_002329"
     CDS             2542..3336
                     /locus_tag="PXD71_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6292397.1"
                     /translation="MALLEVNHLNKRFVERSSLFHKQDFYAVKEVSFTLNRKETLAII
                     GENGAGKSTLAKMVAGMVGPTSGELIFRGTPLSFGDYKYRAKHIRMMFQDPNDAFDPN
                     YNIGQILDAPLRLATDLNEEERNERIFNTLKLVGMYPEQALVPISEASSGQKQRIAFA
                     RALILEPEIIIADDTINSLDFSVKTQLINLMLSVQQRQGIAYIYVGQNLGLIKHIADK
                     IMVMQSGEVVEYGLTKEVLLNPQHQITKRLIESHFGKHLSSYAWAS"
     gene            3478..4116
                     /locus_tag="PXD71_002330"
     CDS             3478..4116
                     /locus_tag="PXD71_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL6292398.1"
                     /translation="MELKHTIEQRKTIKAFNSTIKISRTELEEMVALSQLAPSKANLQ
                     PWRFVIIDVEILKAALSKNVAFNGPPCETASAIVVILADLQYEKLLGAIVDQSVETGC
                     LHKEFRERKFDFLLNLHNQATQAEIRDQVLIDTSLAAMQLMLIAKDKGYDTHAIGVFE
                     RPAVLKLLNIDAERYLPVMLLAIGKAANPALPSCRLPLEYTVSWNDGQGFKI"
BASE COUNT         1308 a          737 c          892 g         1246 t
ORIGIN      
        1 atttatttga tctaggttag cccaaaatga ttagaaactg atataatccg accgttatca
       61 cttttaaatt ttaatctaaa gaggaacaaa ctatgtctgt tattaaaatg actgaccttg
      121 atttagcggg taaacgttta tttattcgtg ctgaccttaa cgtaccggta aaagatggca
      181 aagtaacatc agatgcccgt attgttgcta ccattccaac cttaaaatta gcgttacaaa
      241 aaggtgctaa agtaatggta acttcgcatt taggtcgtcc gacagaaggc gtatttgaag
      301 aggaaaactc tttacaacct gtagttgatt acttaaatgc atcagattta ggtgttcctg
      361 ttcgtttagt gcgtgattac ttagacggtg ttgaagtaaa cgagaatgaa atcgtggtgc
      421 ttgaaaacgt gcgtattaac aaaggtgaga agaaaaacga ccctgagtta gcaaaaaaat
      481 atgcatcact ttgtgatgtg tttgtaatgg acgcattcgg tacagctcac cgtgcggaag
      541 gttcaactta cggtgtggca gaatatgccc ctgttgcgtg tgcaggtccg ttattagcca
      601 ctgaattaga tgcattaggc aaagcattaa aagagccaca acgtccaatg ttagcgattg
      661 tgggtggttc taaagtatct actaaattaa ctgtgcttga ttcgctttca aaaattgccg
      721 accaattaat cgtgggcggt ggtattgcca atacctttat tgccgcagaa ggtcacgctg
      781 tgggtaaatc attatacgaa gaagatttaa tccctgaagc aaaacgttta gcacaagtaa
      841 ccaatattcc tgttccggtt gatgtgcgtg taggtactga attctctgaa actgcaccgg
      901 caacggaaaa agcggtgagt gaagtacgag ctgatgaatc aattttcgat attggtgaaa
      961 aatcgtcaga gcaattagct gaaattatcc gtaattcaaa aaccatcctt tggaatggtc
     1021 cggtcggcgt gtttgagttc cctaacttcc gtaaaggaac agaagtgatt tcaaatgcga
     1081 ttgcagaagc aacggcaaac ggtgcattct caattgccgg tggtggcgat accttagctg
     1141 cgattgattt attcggtatc aaagacaaaa tttcttacat ttcaaccggt ggcggtgcgt
     1201 tcttagaatt cgtggaaggt aaagtattac cggcagtgga aatcttagaa aaacgagcta
     1261 acggctaaca gttacaagcg gtcaaatttt gtgaaaattt tgcaagattt ggccgtatat
     1321 tttaacaatg tgcttttgca catttcatac aaaggaagaa aaaaatgtca ttattaaaca
     1381 tcgtaaaacc gggtgtagta acaggcgacg acgtacaaaa agttttcgct tatgctaaag
     1441 aacacaactt cgctattccg gcagtaaact gcgtgggttc agattcagtt aatgccgtat
     1501 tagaaacagc cgctcgtgta aaatcaccgg taatcgtgca attctcaaat ggtggtgcgc
     1561 agttctatgc gggtaaaggc attaagccgg cttcaggcgc tcgtgcagat gtattagggg
     1621 cgattgcggg tgcaaaacac gttcatcaat tagcagaaga atatggtgtg cctgtgattc
     1681 ttcacaccga ccacgctgcg aaaaacttat taccttggat cgatggctta cttgaagcag
     1741 gcgaaaaaca cttcgcagaa accggtcgtc cgttattctc ttcacatatg atcgatttat
     1801 ctgaagagcc aatcgaagaa aatatggaaa tctgccgtga atatttagcc cgtatggaca
     1861 aaatcggtat gacccttgaa atcgaaatcg gcgtaaccgg tggtgaagaa gatggcgtag
     1921 ataattctga tgtagatgaa tctaaacttt acacccaacc ttctgaagtt ttatatgttt
     1981 acgaccaatt aagcccaatc agcccacgtt tcacgattgc ggcagcattt ggtaacgtac
     2041 acggtgttta caagccgggt aatgtaaaat taaaaccatc aatcttaggt gcttcacaag
     2101 agtatgtatc taaagagcgt ggtttaccgg caaagtcatt agacttcgtt ttccacggcg
     2161 gttctggttc ttcacgtgaa gagattcgtg aagcaatcag ctacggtgca atcaaaatga
     2221 acattgacac tgacacccaa tgggctgcgt gggacggtat tcttcaattc tacaaagcaa
     2281 acgaagcata tttacaaggt caattaggta acccgacagg ccctgatgca ccaaacaaaa
     2341 aatactacga tccacgtgtt tggttacgta aaatggaaga gtctatgtct aaacgcttag
     2401 agcaatcttt cgaagactta aactgtgtga acgttttata atttgcaaaa aattccataa
     2461 atcaggccgc ttgtataagc ggtctttttt tatttatttt ttgcggtaaa ctatcacttt
     2521 atttccaagc accaatttat tatggcatta cttgaagtta atcatctgaa taagcgtttt
     2581 gttgaacgta gtagcctatt tcataaacaa gatttttatg cggtaaaaga agtttctttt
     2641 accctaaatc gcaaggaaac tttagcgatt attggtgaaa acggagctgg gaaatctacc
     2701 ttggctaaaa tggttgctgg tatggttggg ccaacatctg gagaactgat ttttagagga
     2761 acaccgcttt catttggcga ttataaatac cgagctaagc atattcgtat gatgtttcag
     2821 gaccctaacg atgcctttga tcctaattat aatataggac aaattttaga tgctccgctc
     2881 cgcttggcga cagatttaaa tgaagaggaa agaaatgagc gtatttttaa tacgctaaaa
     2941 ttggtaggaa tgtatcctga gcaagcgctc gttcctattt cagaggcttc cagtgggcaa
     3001 aagcagcgta ttgcgtttgc tcgtgcttta attttagaac cggaaattat tattgcggat
     3061 gatacgatta attcactgga tttttcagtc aaaacacaat taattaattt aatgttaagt
     3121 gtccagcagc gacaagggat tgcttatatt tatgtggggc aaaatttagg tctgatcaaa
     3181 catattgccg ataaaataat ggtgatgcaa agtggcgaag tagtagagta tggcttgacg
     3241 aaagaggttt tactgaatcc gcaacatcaa attacgaaac gtttaattga aagtcatttt
     3301 ggaaagcacc ttagttccta tgcttgggca agctaaatct ttactcctgc tctaatatga
     3361 ttacttgata attttgacct atttcaataa atatgcagaa taactctttt caattattac
     3421 attcctaatc tttaaattat agactatctc aattgtttga gagataggag aaaagagatg
     3481 gaattgaaac atacgattga acagcgtaaa actatcaaag cttttaattc aacaattaag
     3541 attagtagaa ctgaattaga agaaatggtc gctttatccc agcttgctcc ctcaaaagca
     3601 aatctgcaac cttggcgttt tgtcattatt gatgttgaaa ttcttaaagc agcattatca
     3661 aaaaatgttg cctttaatgg cccaccttgt gaaacagcgt ctgctattgt ggtgatattg
     3721 gcagatttgc aatatgaaaa attattaggc gcaattgttg atcaatcggt agaaactggg
     3781 tgtttgcata aagagtttcg tgaacgtaaa tttgattttt tactaaatct tcataaccaa
     3841 gcaactcaag cagaaattag agatcaggta ttaattgata ctagtttggc ggcaatgcaa
     3901 ttaatgctaa ttgccaaaga taagggctat gatactcatg cgataggcgt gtttgagcgc
     3961 cctgctgtat taaaattatt gaatatagat gcagaacgtt atttgcctgt tatgctacta
     4021 gctatcggta aagcagcaaa cccagcgttg ccaagttgcc gtttacctct agaatatacg
     4081 gtttcttgga acgatggaca aggttttaaa atataaggtg caagcggtta aatatcttaa
     4141 ttttttggca aaaaattgtt aaaatttgag cgttgtaatt tat
//
LOCUS       DANLXS010000197         4175 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.200, whole genome shotgun sequence.
ACCESSION   DANLXS010000197 DANLXS010000000
VERSION     DANLXS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.200"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            166..354
                     /locus_tag="PXD71_002331"
     CDS             166..354
                     /locus_tag="PXD71_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292399.1"
                     /translation="MSYDANDALNEIEEALSELERVAEDLINNNPNKESELRGQGVHQ
                     ATKHLRFRIRNIRRGEAI"
     gene            395..697
                     /locus_tag="PXD71_002332"
     CDS             395..697
                     /locus_tag="PXD71_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292400.1"
                     /translation="MKIKLYFPDESIATIKRMGLRQMSPETLRWTADHPSSSYGMGAL
                     LRGKSGEILDGKSFAAMVHAFGAWIETDSEDTSRRVHNALVTAATGTEESVKVAKE"
     gene            697..2007
                     /locus_tag="PXD71_002333"
     CDS             697..2007
                     /locus_tag="PXD71_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL6292401.1"
                     /translation="MSNILNWPDYKVLQVSELEHDYQVHAEVSEPPTQCPHCNHPEIV
                     GFGRRDEVIMDTPVHGRRTGIMLNRRRYRCQSCRKTFLEPVPHKDEKRQMTNRLIQYI
                     ERESLRRTFSSVAEDVGVDEKTVRNIFNDYCERLEKTLNFEMPQWLGIDEIHIIKPRC
                     VITNIQQQTIVDMLDNRNKTTVTRYLSKRTDRDLVRYVAMDMWRPYRQAVETMIPDAT
                     VIIDKFHVVRMANESLERARKAIRSALTPQQRRGLMRDRFVLLKRRHELTDAEYMRFS
                     GWTLNYPEIGQAYELKEAFFEIWDCQTRHQAQEAYYSWLRQITPEMKAHYDPLIKAMG
                     NWHDDIFAYFDHPITNAYTESLNNLIRVVNRVGRGYSFEALRAKILFTEGFQKIKKPR
                     YQRQRIPEGAMGRMPFYGVAEAGPSTNYGADISTLVREIEAGRL"
     gene            complement(2103..3305)
                     /gene="tet(H)"
                     /locus_tag="PXD71_002334"
     CDS             complement(2103..3305)
                     /gene="tet(H)"
                     /locus_tag="PXD71_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL6292402.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
     gene            3397..4020
                     /gene="tetR(H)"
                     /locus_tag="PXD71_002335"
     CDS             3397..4020
                     /gene="tetR(H)"
                     /locus_tag="PXD71_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(H)"
                     /protein_id="HDL6292403.1"
                     /translation="MAKLDKEQVIDNALILLNEVGIEGLTTRKLAQKIGVEQPTLYWH
                     VKNKRALLDALAETILQKHHHHVLPLPNETWQDFLRNNAKSFRQALLMYRDGGKIHAG
                     TRPSESQFETSEQQLQFLCDAGFSLSQAVYALSSIAHFTLGSVLETQEHQESQKEREK
                     VETDTVAYPPLLTQAVAIMDSDNGDAAFLFVLDVMISGLETVLKSAK"
     gene            complement(4023..4160)
                     /locus_tag="PXD71_002336"
     CDS             complement(4023..4160)
                     /locus_tag="PXD71_002336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292404.1"
                     /translation="MPENGVIFSDGIEDDYLQFNAGCIAHIGIADIQGQLISQKGRQR
                     I"
BASE COUNT         1231 a          952 c          980 g         1012 t
ORIGIN      
        1 cggatttagc ggttgatctg cgctagacgt aattttgtaa ataaaccgga ttttagggtt
       61 gacggaaaag gccagatgcc ctaatattag attcaagagc tgagaacaac ggctcgggca
      121 taagcccaac gggcgcaccg ccgaacttgg tgcaggatag aagccatgtc atacgatgca
      181 aatgatgcgc tgaatgaaat cgaagaggcg cttagcgagc ttgaaagggt tgctgaagac
      241 ctaatcaaca acaacccgaa taaagaatca gaattacgcg gccaaggggt tcaccaagcg
      301 acaaagcacc tccgttttcg tattcgcaac atccgccgcg gcgaagcaat ttgactaaca
      361 tgccgcccta ggggcggctt tcagcgaggt cagcatgaaa atcaaactct attttccgga
      421 cgagtctatc gccacgataa agcgaatggg gcttcgccaa atgtctcccg agactctcag
      481 gtggactgcc gatcatccgt caagcagcta tggtatgggc gctttgctca gaggcaagtc
      541 tggtgaaatc cttgatggga agtcttttgc tgcaatggtg catgcctttg gcgcgtggat
      601 tgaaacagac agtgaagata ccagtcgtcg agttcataac gcgctagtca ctgctgccac
      661 tggcaccgag gaatcggtta aggtggcaaa ggagtaatgt cgaatatcct caactggcct
      721 gattacaaag tgctccaggt atccgaactg gagcacgatt accaggtaca cgccgaagtt
      781 tccgaaccgc ctacgcagtg cccccactgc aatcatcctg aaatcgtcgg ctttggtcgc
      841 cgtgatgagg tgatcatgga tacacccgtc cacggaaggc gtaccggcat catgcttaac
      901 cgccgtcgct atcgctgcca gtcttgccga aagacgtttc tggagcctgt accgcacaaa
      961 gacgaaaagc ggcagatgac caaccgcctg atccagtaca tcgagcgcga gagcctgcgc
     1021 cgcacgttct ccagtgttgc cgaggatgtg ggcgtcgatg aaaagaccgt ccgtaacatt
     1081 ttcaacgact attgtgaacg cctcgaaaag acgctcaatt ttgaaatgcc gcagtggttg
     1141 ggtatcgacg aaatccacat catcaaaccc cgctgcgtga tcaccaacat tcagcagcag
     1201 accattgtgg acatgctgga taaccgcaat aaaaccaccg tcacccgcta cctgagcaaa
     1261 cgcactgacc gcgacctggt gcgctacgtg gcaatggata tgtggaggcc gtaccggcaa
     1321 gcagtagaaa ccatgattcc tgacgccacg gtgatcatcg acaagttcca cgttgtcagg
     1381 atggcgaacg aatcactgga acgcgccaga aaagccatca gaagcgccct gacgccacag
     1441 cagcgccgtg ggttgatgcg agataggttt gtcctactga aacgccgtca cgagctgaca
     1501 gatgccgaat acatgcgttt ttcaggctgg acattgaatt accctgagat tggccaagcc
     1561 tacgaactga aagaagcctt tttcgagatt tgggactgtc agacccggca ccaggcacag
     1621 gaggcatatt actcttggct gcgacagatt acgccggaaa tgaaagccca ctatgacccg
     1681 cttatcaaag cgatgggcaa ctggcacgat gacattttcg cttacttcga ccaccccatt
     1741 accaacgcct acaccgagtc actgaacaac ctgattcgtg ttgttaatcg tgttggccgt
     1801 ggctacagct ttgaagcgct gcgagccaag atcctgttta ctgagggctt ccagaagatc
     1861 aagaagccac gctatcagcg tcaacggata cccgagggcg ctatgggcag gatgccgttc
     1921 tatggcgttg ccgaagcggg gccatccact aactacggcg ctgacatatc aacactggtg
     1981 cgggagattg aggcggggcg tttatagccc tgtttcaacc ctgtaatccg gatacccaaa
     2041 taaagtccta tcaacgttat taagagcgga caggactttt atgatcttta taatttctag
     2101 tattaagggg ttgaaataac agctttaggt gtggtttttc tttggtgaaa ataagcggta
     2161 ataagcaaca tagcataaag tattgccccc atcagccata acagaccatc ccaataagcg
     2221 acgctataac tataaataaa ggcaaataaa aggggaccaa tgatcccggt aatattggtt
     2281 aggctcacca gagtaccttg taatttccct tgcgcattat catcgacaga ttttgataaa
     2341 taaccttgta atgcgggttg ccccatacct cctgccgcta agcaaattaa tgctggtaag
     2401 atgacccaaa cgtggcctat ccacgctaat aataaacagc ccatcatatc aatagacata
     2461 ctgatcataa tggtggtttt ttcgccccat ttttgtgcca atttcccagc gacaatcgcc
     2521 tgaaagaaaa tatttaatac acccagaacc gccaaagaca taccgataga agttgtgttc
     2581 caatcaaaac gatattgtgt aaacagcacc cagatggtgg caggaatttg cccgataagc
     2641 tggataataa aataggttgc taaccaaaag tagaggcttt tcttaaaaaa aacagtgact
     2701 gtatttgagg cagtttggtt ttcaggtgtc ctattggcaa caagcgcttc tcttttttgt
     2761 gtttctcgga aaaagagcaa agagagtatt aataatatcg agtgtgaaat agcggcaaaa
     2821 ataaatggca tatgagcact gatatcacct aataatcccc ctagcattgg gccgataata
     2881 aggccaacac caaaagcacc acctaagaaa ccaaaatagc gagttcgatt tttagcggga
     2941 gtcacatcac tcatcgctga tgcacatacg gcacctgttg cgcctgtgat ccccgcaatg
     3001 atgcgcccaa tatagagcat ccaaagtgtg gttgagaatg ccattaaaag atagtcgagt
     3061 gccgcgccta aaagggaaaa cagcaagatg ggttttctgc cgtatttatc agacagtcgt
     3121 cctagaatag gagcaaaaat aacctgcatg gtagcataga gcgctaatag cacaccgtaa
     3181 tgggttgcca gtgaattttc actgacaaat tcatttaata gagtagggag tactggcatg
     3241 ataagcccga taccaatggc atctaatacg gtgatcagca gtataataat aattgattta
     3301 ttcattgaga tcctaaaaat ctatcagtga tagagtgggt gtaatatatc tctatcagtg
     3361 atagattgtc aaggctattt tattttgagg taggtaatgg caaagctaga taaagaacaa
     3421 gttattgata atgcgttgat tttacttaat gaagttggta ttgaaggatt aacaacgcgt
     3481 aagctggcgc aaaaaatagg tgtggaacaa cccacattgt attggcatgt aaaaaataaa
     3541 cgcgctttgt tagatgcatt agcagaaact attttgcaaa agcaccatca tcatgttttg
     3601 ccattgccga atgaaacatg gcaggacttt ttgcgaaata acgcgaaaag cttccgccaa
     3661 gccttattaa tgtatcgtga tggtggcaaa attcatgcgg gaacacgccc ctctgaaagt
     3721 caatttgaga catcagaaca gcaactacag tttttgtgtg atgctgggtt tagtctatct
     3781 caagccgtgt atgcattaag ctctattgcg cattttacat taggctccgt actggaaact
     3841 caagagcatc aagaaagcca aaaagagcgt gaaaaagtag agacggatac tgttgcctat
     3901 ccgccattat taacccaagc cgttgcaatt atggatagtg ataatggtga tgctgcattt
     3961 ttgtttgtcc ttgatgtgat gatctctgga cttgaaacag tattaaagag cgctaaataa
     4021 atctaaattc gctgacgccc tttttggcta attagttgcc cttgtatgtc agcgatacca
     4081 atgtgagcaa tacaacctgc attaaattgt aaatagtcat cttcgatgcc atcagagaaa
     4141 attacgccat tctctggcat caaagatcct aattg
//
LOCUS       DANLXS010000198         4101 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.201, whole genome shotgun sequence.
ACCESSION   DANLXS010000198 DANLXS010000000
VERSION     DANLXS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.201"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            112..1641
                     /gene="mepM"
                     /locus_tag="PXD71_002337"
     CDS             112..1641
                     /gene="mepM"
                     /locus_tag="PXD71_002337"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDL6292405.1"
                     /translation="MQHVILARDRKRHKKHFKIAVFLLAVFSLFVDIVLALKDQSSIN
                     NTSSQEDIIAIDKQEKYQSINTNLAENNTLHQEQHSSFINYVTDLLTQSALVIEHDDE
                     IVDDQTATSYEDELVGQDDEVEEGIETVENQVPGEKLPEEAEKALEDLLDVADQALRI
                     QNQFSYTVARGDKLKDVLEQSGLTASVAKNLIKQHSALAQLEPGQQFYWVLDNDGELE
                     YMNWLVSEKEERVYERKENGKFSFQRFEKKGEWKLDVVRGTIQNSFAVSLKAVGLSDR
                     QINQLAVGLQSQIATNKLKKGDRFAILIKREYIDGKVTDLGNVEGIHVISSKKSYYAI
                     QADNGRYYSRYGETLSGGFARHPLLFAARVTSSFNPKRRHPITGRISPHKGVDYGVPI
                     GTPIIAPSDGVVEHVAYQARGAGRYIRIKHGHITTVYMHLSKTLVKPGQSVKKGERIA
                     LSGNTGGSTGPHLHYEFHINGRPVNPITVKLPGSNSGMGNKERQAFLNKAKNVESRLK
                     M"
     gene            1651..2334
                     /gene="dsbC"
                     /locus_tag="PXD71_002338"
     CDS             1651..2334
                     /gene="dsbC"
                     /locus_tag="PXD71_002338"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDL6292406.1"
                     /translation="MKKNLITLGLMCLSTITFASDAKLKSQLEKIGATDVKISDSPLT
                     GFKMATSNEGVVYISQDGRYVIQGKVFELKNGKAIDITNQGLLAELNSYTNEMIVYPA
                     KNEKHVITVFMDITCHYCHLLHTKIKEYNDLGITIRYLAFPRGGMNTQTAKQMEAIWT
                     SADKVKALNDAEDGKLPTQIKTPDIVKKHYQLGIKFGVTGTPNMVTSEGELIGGYVEP
                     KELAKMLSE"
     gene            2419..>4101
                     /gene="recJ"
                     /locus_tag="PXD71_002339"
     CDS             2419..>4101
                     /gene="recJ"
                     /locus_tag="PXD71_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL6292407.1"
                     /translation="MEKIIKRRPLLTTEIVSTNPLLNRIYQSRGIKNTQELEHNLKNL
                     HRPQQLANIEQAVDLLLQAFREKSRIIIVGDFDADGATSTSLALIALRQLGFDKVDYL
                     IPDRFSQGYGLSVAVAEMVLAKGADLVLTVDNGISSTEGIALLKSHQIQVLVTDHHLP
                     PESLPNADALVNPNLAFCEFPSKSLAGVGVIFYVMMALRSRMREQHLFENKPEPNFAE
                     LLDLVALGTVADVVPLDQNNRILVHQGLQRIRSGHCRLGIKALAEVGKRDLSTLQAAD
                     LGFAIAPRLNAAGRLENMSLGVELLICENMELARRLALDLDSLNQTRKEFEQEMKAEA
                     LSICANLPSLAQSEQAHGIVLYQADWHQGVIGILASRIKDQFNRPVIAFAQESEESEY
                     LKGSARSINGVHMRDLLEHIDMRHPDLIEKFGGHAMAAGLTIHQSKLDLFKQIFDQSI
                     NAIIEPEQLQGIIYTDGELNALEFTLANAEMIRQGGPWGQHFPEPSFEGEFSILQQKL
                     LAGKHLKLMLQSEKGQLFDAIWFNVDVRAFPDLSIKKARLVYRLDINEFRGEK"
BASE COUNT         1376 a          651 c          896 g         1178 t
ORIGIN      
        1 agctgatttt tcgtgttgtg acaatagatt aaatgaattt tttcttggta aaatagtcta
       61 ctcagaaaaa tatgtaaatt ttagtaaatt ttaaaaatag cgggatatat tttgcaacat
      121 gtaattttag cgagagatcg taagcgacat aaaaaacatt ttaagattgc ggttttctta
      181 ttagcggtat ttagtctatt tgttgatatt gttctcgcac taaaagatca atcctcaatc
      241 aataatacat cttcacagga agatattatt gccattgata aacaggaaaa atatcagagt
      301 atcaacacta atttggcgga aaataatact ctccatcagg agcaacattc cagttttatt
      361 aattatgtga ctgatttact gactcagtcg gcattagtaa tcgaacatga cgacgaaatt
      421 gttgatgatc aaacagcgac ttcttatgaa gatgaattag ttgggcaaga cgatgaagta
      481 gaagaaggga ttgaaacggt tgaaaatcaa gttcctgggg aaaaactccc agaagaagct
      541 gaaaaggcat tagaagattt acttgatgtg gctgatcaag cactacgaat tcaaaaccaa
      601 ttcagttata ccgtagctcg aggtgataaa cttaaagatg tattagagca atcaggctta
      661 actgcatccg ttgcgaaaaa tttaattaag caacattcgg cattagcaca attagaaccg
      721 ggtcaacaat tttattgggt attagataat gacggtgaat tagaatatat gaactggctg
      781 gtttccgaaa aagaggaacg ggtttatgag cgaaaagaaa atggtaaatt ttcttttcaa
      841 agatttgaga aaaaagggga atggaagtta gatgttgtaa gaggaactat tcagaatagt
      901 tttgcggtta gcttaaaagc cgttggctta tcagaccgcc aaattaacca gctagcggtt
      961 ggattgcagt cacaaattgc gacgaataaa ttaaaaaaag gggataggtt tgcaattctg
     1021 attaagcggg aatatattga cggcaaagta acggatttag gaaatgtgga aggaatccat
     1081 gtaattagca gtaaaaaaag ttattatgct attcaagcag ataatggacg ctattatagt
     1141 cgatatggtg aaacattaag tggaggattt gctcgtcatc cattattatt tgcagcaaga
     1201 gtaacttctt ctttcaaccc caaacgcaga caccctatta cagggagaat tagtccgcat
     1261 aaaggggttg actatggcgt gccaataggt actccgatca tcgcaccaag tgatggtgtc
     1321 gtagagcatg tggcatatca agcccgtgga gcaggacgtt atattcggat aaaacacgga
     1381 catattacga ctgtttatat gcacctcagc aaaacgttgg taaaaccagg gcagagtgtt
     1441 aaaaaagggg agcggatagc attatcgggt aatacaggcg gttcaaccgg gcctcattta
     1501 cactatgaat tccatattaa tggtcgaccg gtcaacccga ttacggtaaa attgcctggc
     1561 tcaaattcag gtatgggtaa taaagaacgt caagcattct taaataaagc gaagaatgta
     1621 gaaagtagat taaaaatgta aggattctca atgaaaaaaa atctaatcac tttaggatta
     1681 atgtgtttat caaccatcac gtttgcttca gatgcaaaac taaaaagcca gcttgaaaaa
     1741 attggagcca cagatgttaa aatttcagat tctccattaa caggctttaa aatggcgact
     1801 tcaaatgaag gtgttgtata tatcagccaa gacggtcgtt atgtaattca aggtaaagta
     1861 tttgagctaa aaaatggtaa agcgattgat attactaatc aaggattgtt ggcagaattg
     1921 aattcataca ctaatgaaat gatcgtatat ccagcgaaaa atgagaaaca tgtgattacg
     1981 gtgtttatgg atattacttg ccattattgc catttactac atactaaaat caaagaatat
     2041 aatgatttag gtattaccat tcgctatttg gcattcccgc gtggtggaat gaatacccaa
     2101 acagcaaaac aaatggaagc tatttggaca tcagcagaca aagtaaaagc actgaatgat
     2161 gctgaagatg gtaaattacc aacccaaata aaaacgccgg atattgtgaa aaaacattac
     2221 caattaggga ttaaatttgg tgtgacaggt accccaaata tggtaacttc agaaggtgag
     2281 ttgattggtg gttatgtaga gcctaaagaa ttagcgaaaa tgttaagtga gtaattcatt
     2341 aacttgaata caagcggtct ttttttgtaa aatttttgca aaagaagacc gcttgttgtt
     2401 tcattaaaga agagtattgt ggaaaaaata attaaaaggc gcccattatt aacaacggaa
     2461 atagtctcta caaacccgtt attaaatcga atctatcaaa gtcgaggcat aaagaatacg
     2521 caagaactag agcataattt aaagaatttg catagaccac agcaacttgc caatattgag
     2581 caagccgttg acttgctatt acaagccttt cgtgaaaaaa gccggatcat tattgtgggt
     2641 gattttgatg cagatggtgc aaccagtaca tcgcttgccc ttattgcgtt acgccaattg
     2701 ggtttcgata aggtagatta tttaattccg gatcgttttt ctcaggggta cggattgagt
     2761 gtagcggttg ctgaaatggt attagcaaaa ggggcggatt tagtcttaac ggtagataat
     2821 ggaatttctt cgactgaagg cattgctctg ctaaaaagcc atcagattca agttttggtt
     2881 accgatcatc atttaccgcc agagagccta cccaatgcag atgcactggt aaaccccaat
     2941 ttagcttttt gtgaatttcc gtctaaatct ttagccgggg taggcgtgat cttctatgta
     3001 atgatggcat tgcgttctcg tatgagagaa caacatttat ttgaaaataa accagagcca
     3061 aattttgctg aattactgga tttagtcgct ttaggcacag tggctgatgt agtgccgtta
     3121 gatcaaaata atcggatatt ggtgcatcaa gggttacagc gaatccgttc agggcattgc
     3181 cgtttaggga taaaagcttt agcggaggtg ggcaagcgag atctttctac tttgcaagca
     3241 gcagatttgg gttttgctat cgcaccacgt ttgaatgcag ctggtcggtt agaaaatatg
     3301 tctttagggg ttgagttact aatttgtgaa aatatggaat tagctcgccg gcttgcgctt
     3361 gatcttgatt ctcttaatca aacccgaaaa gagtttgaac aagaaatgaa agcagaagca
     3421 ctgtcgattt gtgcgaattt gccaagtctt gcgcagtctg aacaggcaca cggaatagtg
     3481 ttatatcaag cagattggca ccaaggtgta attgggatct tagcttctcg cattaaagat
     3541 caatttaacc gtcctgtcat tgcttttgca caagaaagtg aggaaagtga atatctaaaa
     3601 ggttctgctc gctcgatcaa cggagtacat atgcgagatt tactggaaca tatcgatatg
     3661 cgacatccag acttgattga aaaatttggt ggacacgcaa tggcggctgg tttgactatc
     3721 catcaatcaa aattagattt atttaaacag atatttgacc aatctattaa tgcaattatc
     3781 gagcctgagc aattacaagg catcatttat accgatggtg agttaaatgc actagagttt
     3841 actttagcca atgccgagat gattaggcaa ggtgggcctt ggggacaaca ttttccagaa
     3901 cctagttttg aaggggagtt tagcattctc caacaaaaat tactcgcagg taaacattta
     3961 aaattaatgt tgcaatcaga gaaagggcaa ctatttgatg caatttggtt taatgtggat
     4021 gtaagggcgt ttcctgactt atcgatcaaa aaggcaagat tagtttatag gttggatatt
     4081 aatgaatttc gaggagagaa a
//
LOCUS       DANLXS010000199         4089 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.202, whole genome shotgun sequence.
ACCESSION   DANLXS010000199 DANLXS010000000
VERSION     DANLXS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.202"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(95..1453)
                     /locus_tag="PXD71_002340"
     CDS             complement(95..1453)
                     /locus_tag="PXD71_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL6292408.1"
                     /translation="MANQQQERQSWSNRLAYIMTVAGATVGFGATWRFPYLVGEHGGG
                     AYVFLFCIAMIVIGIPMILVENVIGRRMRVNAIDAFAGFANGKKIASGWKILGYMGLL
                     GAFGILAYYMVLGGWVLNYIGSLLTGTLDLSQPVTVGTTQQFFQQSIFNSPMTIIAYT
                     ALFVLVNYFILVKGIVDGIERSVKYLMPILFIFLIVMVVRNITLPGAAEGIAFYLMPD
                     FSKITPKLFVFVLGQVFFALSLGFGVLITLSSYLDKSENLVKTATITGVMNTLIAVLA
                     GFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSNMWGGTVFAVIFFSLLVVAALTTSLTI
                     YEVIITALQEKAKISRQKAIFITLATIFLLGNIPSALSDNILQDVQVMGKSIFDAFDY
                     ISGNILFILTALGSAIFVGFVLKEEAKEELGAGKMFTTIWFNYVRFVIPVIILVIFFN
                     SL"
     gene            complement(1523..2350)
                     /locus_tag="PXD71_002341"
     CDS             complement(1523..2350)
                     /locus_tag="PXD71_002341"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDL6292409.1"
                     /translation="MNHPKHRAFLKWAGGKYRLIPDIQTHLPKKACLVEPFVGAGSVF
                     LNTDFERYILADINPDLINLFNVVKADVEEYIAQTKQLFLHPQANTEAFYKARRAEFN
                     RSKDIFRRSVIFLYLNRFGYNGLCRYNQKKGYNVPFGRYKTHYFPEKELRFFAEKAQK
                     ATFVCADFEQVFELAKNQLTDYVIYCDPPYAPLAQETNFTQYAGGGFSLEQQGRLATL
                     ALEMKGLGVPVLISNHDTDFTRRIYQSADITQIQVQRSIGQKVGSRVKVGELFALFI"
     gene            complement(2364..3452)
                     /gene="aroB"
                     /locus_tag="PXD71_002342"
     CDS             complement(2364..3452)
                     /gene="aroB"
                     /locus_tag="PXD71_002342"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL6292410.1"
                     /translation="MLQVNVELKERRYPIIIGAGLLNQPASYSPLKSGDKVMIVSNPT
                     VATHYLSVVTNALKELGCHVDSVLIPDGEEYKTLESLNLIFTALLEKNHNRDTTLIAL
                     GGGVIGDVAGYAAASYQRGIRFIQIPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     VSVIVDTNTLHTLSKREVSAGLAEVIKYGAIFDVTFFEWLEKHIDDLVSLKQDELEYC
                     IQRCCQLKADVVARDETEKGDRALLNLGHTFGHAIEARMGYGVWLHGEAVSVGMLEAA
                     ELSRILGDLSPQEVARLEKLLARANLPTISPDGMEPAEYLPYMWRDKKVLAGQLRLIL
                     LKSLGEAYVSSQATESQVLASIERFTQR"
     gene            complement(3476..3997)
                     /gene="aroK"
                     /locus_tag="PXD71_002343"
     CDS             complement(3476..3997)
                     /gene="aroK"
                     /locus_tag="PXD71_002343"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDL6292411.1"
                     /translation="MAEKRNIFLIGPMGAGKSTIGRQLAQTLGMEFMDSDSVIEERAG
                     ADIDWIFDVEGEEGFRKREERILNELTQNYGIVLSTGGGSILSKDNRNVLSARGIVIY
                     LETTVDKQFERTQRDKKRPLLQTEDPRKTLEELAKIRNPLYEEIADITIHTDEQSAKV
                     VANQIIDMIDNLQ"
BASE COUNT         1335 a          923 c          682 g         1149 t
ORIGIN      
        1 taattaagca agaattgcaa attccccaca aaatttaacc gcttgtcact aaaaataaag
       61 ctgtttgaaa atccattcaa acagcttata gaatttagag tgaattaaag aaaatcacca
      121 agataatcac cggaatcaca aagcgaacat agttaaacca aatagtggta aacattttac
      181 ccgcacctaa ttcctccttc gcctcttctt ttagaacaaa gccaacgaaa atcgcgctac
      241 ctaaggcggt taaaataaac agaatattgc cactaatata atcgaaggca tcaaaaatgc
      301 tcttgcccat aacctgcaca tcttgcaata tattgtcact taatgctgat ggaatattac
      361 ccaacagaaa aatagtagcc aaagtaataa aaatagcttt ttgacggcta atctttgcct
      421 tctcttgcaa tgcagtgata ataacttcat aaatagttaa cgaggttgtt aatgccgcca
      481 ccactaatag actaaaaaag ataaccgcaa aaactgttcc cccccacata tttgagaaca
      541 caatcggcaa actttggaat actaaagtcg gacctgaatt tggagcaacg ccaaagctaa
      601 ataatgacgg aaaaatcata aaaccggcaa gtaccgcaat taaggtgttc atcaccccag
      661 taattgttgc tgtttttact aaattctcgc tcttatcaag gtagcttgaa agggttatca
      721 acacaccaaa accaaggctt aatgcaaaaa atacttgacc cagcacaaac acaaacaatt
      781 ttggtgtgat ttttgaaaaa tcgggcatca gataaaaggc aatgccttca gcagcccccg
      841 gtaaggtaat atttcgaacc accatcacaa tcaggaaaat aaacagaatc ggcatcaaat
      901 atttcactga acgctcaata ccgtccacaa tgccttttac caaaataaaa taattcacca
      961 atacaaaaag tgccgtataa gcaataattg tcattgggct attaaaaata ctttgttgga
     1021 agaattgttg ggtcgtgcca acggtaacag gttgagacag atctagtgtc cccgtgagta
     1081 agctaccgat ataatttaat acccaaccac caagtaccat atagtaggca agaataccaa
     1141 atgctccaag caagcccata tagccaagaa ttttccaacc tgaggcaatt tttttaccat
     1201 tagcaaaacc ggcaaaagca tcaatagcat tcactctcat tcggcgacca attacattct
     1261 ccactaatat catagggata ccaattacga tcatcgcaat acaaaataaa aacacataag
     1321 caccgcctcc gtgttccccc acaagataag ggaaacgcca agttgcacca aaaccgactg
     1381 ttgcaccagc aacggtcata atatacgcca atcggttaga ccaagattgg cgttcttgtt
     1441 gttgatttgc catttaatac tccatcggct aacaataccg cttcaaccaa caagcggtca
     1501 gatttttaga aaatattaca aattaaatga agagtgcgaa taactcgcca actttaaccc
     1561 ttgagcccac cttctgccct attgaacgct gtacctgaat ttgggtaata tcggcagatt
     1621 gataaatcct acgggtaaaa tcagtatcgt gatttgaaat taatacggga acgcctaatc
     1681 ctttcatttc taaagccagt gttgccaaac gcccttgttg ctctaggcta aaaccaccgc
     1741 cagcatattg cgtaaaattc gtttcttgtg ctaaaggagc gtaaggtggg tcgcagtaaa
     1801 tcacataatc cgttaattga ttttttgcca actcaaagac ctgttcaaaa tccgcacaca
     1861 caaaagttgc cttttgtgct ttttcagcaa aaaaacgtag ttctttttct ggaaaataat
     1921 gggttttata acgcccaaag ggaacattgt accctttttt ctggttataa cggcaaagtc
     1981 cgttatagcc aaatcgattt aaatacaaaa aaatcaccga gcgacgaaaa atatcttttg
     2041 agcgattaaa ctccgcacgg cgtgctttat aaaaggcttc tgtattcgct tgtgggtgaa
     2101 gaaacagttg ctttgtttgc gcaatatatt cctcaacatc tgctttcacc acgttgaaaa
     2161 gattaatcaa gtcaggatta atatcagcaa gaatataacg ttcaaaatct gtattcagaa
     2221 agaccgaacc tgcaccaaca aaaggctcga ctaaacaagc cttttttggc aaatgggttt
     2281 gaatatctgg aattaagcga tatttaccgc cagcccattt taaaaaggct cgatgcttag
     2341 ggtgattcat ttcattttaa acattaacgt tgggtaaaac gctcaataga ggctaatact
     2401 tgagattctg tggcttgcga tgaaacgtaa gcttcaccaa gtgatttcaa taaaattagg
     2461 cgtaattgac ctgccagtac ttttttatct cgccacatat aaggtagata ttctgccggt
     2521 tccattccat caggtgaaat tgttggcaaa ttggctcggg ccaataattt ttccaagcga
     2581 gcaacctctt gaggacttaa atcccccaga atacgagata attccgcagc ctctagcata
     2641 ccaaccgaaa cagcctctcc gtgtaaccat acaccatagc ccatacgtgc ttcaatggcg
     2701 tgaccaaagg tatgtcctaa gtttagtaat gcacgatctc ctttttccgt ttcatctcga
     2761 gcaaccacgt cagcttttag ctggcaacaa cgctgaatac aatattccag ctcatcttgc
     2821 ttgagactga ccaaatcatc aatatgcttt tcaagccatt caaaaaaagt aacatcaaag
     2881 attgcaccgt atttgatgac ttccgccaaa ccagcactga cttctctttt ggataaggta
     2941 tggagcgtat tggtatcaac aatcacagaa acaggctgat aaaaagcccc aatcatattt
     3001 ttaccgagtg ggtggtttac tgccgttttc ccgcctactg aagaatcaac ttgagccagt
     3061 aaagtggtcg gaatttgtat aaaacgaata ccacgttggt aagaggcagc tgcatagcct
     3121 gcaacatcac cgattacacc accgcctaaa gcaattaaag tggtatcgcg attatgattt
     3181 ttttctaata atgcggtaaa aatcaaattt aatgattcta gcgttttata ttcttcaccg
     3241 tcgggaatca ataccgaatc aacgtggcaa cccagctcct ttaacgcatt tgttaccaca
     3301 ctaagataat gtgtcgccac cgttggattt gagacaatca tcaccttatc tccagatttt
     3361 aatgggctat aactagcagg ctgatttaat aaccctgcac caattataat cgggtaacga
     3421 cgctctttta attccacatt gacttgtaac atatcttagc tcctttctta cctggctact
     3481 gtaaattatc aatcatatca ataatttgat ttgccactac tttagcgctc tgctcgtccg
     3541 tatgaatagt aatatctgca atttcttcat acaatgggtt acggattttc gctaactctt
     3601 ccaacgtttt acgggggtct tctgtttgca ataaagggcg ttttttatct cgctgagtac
     3661 gttcaaactg tttatccact gtcgtttcta aataaataac gataccacga gccgataaca
     3721 cattacggtt atctttagat aaaatcgaac caccgccagt tgaaagcacg ataccataat
     3781 tttgcgtcag ttcatttaga atacgttctt cacgcttacg gaaaccctct tcaccttcaa
     3841 catcgaagat ccaatcaata tccgcacctg ctcgttcttc gatcacactg tcggaatcca
     3901 taaattccat acctaatgtt tgagcaagct gacgaccaat cgtactttta cctgctccca
     3961 tcggcccaat caaaaaaata ttacgttttt cagccattat ttcttcttca ttttaaaatt
     4021 tactaaacat ttgatctgtg aaattaaaag aaaaaatgac cgcttgttct acaagcggtc
     4081 tgatttctt
//
LOCUS       DANLXS010000200         4037 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.203, whole genome shotgun sequence.
ACCESSION   DANLXS010000200 DANLXS010000000
VERSION     DANLXS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.203"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(74..1765)
                     /gene="dld"
                     /locus_tag="PXD71_002344"
     CDS             complement(74..1765)
                     /gene="dld"
                     /locus_tag="PXD71_002344"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDL6292412.1"
                     /translation="MSNNSLISQLTEIVGQSYIITDPTKTEAYRSGYRFGTGSAIAVV
                     KPTTLLEYWNVLKACVAADVIVISQAANTGLTGGSTPSGNDYDRDIVIINTMRMNGIQ
                     LINNANQVVCLPGSTLNELELLLKTHGREPHSVIGSSCIGASVIGGICNNSGGALVQR
                     GPAYTEMALFAQLNEDGTLELKNHLGIDLGDTPEEILENLQAQRYQRKDIQQACGKGH
                     DHAYCNHVRQIDEDSPARFNADPARHYEAAGSAGKLAVFAVRLDTFPLEKDTAVFYIG
                     TNHTDTLNDIRRYMLANFEVLPISGEYIHRDAFDIAADYGKDTFWVIKKLGTHRLPQL
                     FSLKAKVDRMAKKLTFLPANLSDKILQAISKMLPQHLPESLRDYRNQYEHHLIIKMGG
                     KGVQEARDYLARYFADTQKGGYLECNPTESQAAMLHRFAVASAAIRYRAIHDKEVEDI
                     VALDIALRRNDKAWFEVLPPEIESQITHKLYYGHFMCHVFHQDYIVKKGVDCLALEHQ
                     MLELLDKRGAQYPAEHNVGHLYEAKPTLRKFYRQLDPTNSFNPGIGQTSKKKYWK"
     gene            complement(1977..3074)
                     /locus_tag="PXD71_002345"
     CDS             complement(1977..3074)
                     /locus_tag="PXD71_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL6292413.1"
                     /translation="MFYSSNPLIKHKTGLLNLAEELGNISQACKVMGMSRDTFYRYQQ
                     AVEQGGVEALLNQNRRVPNIKNRVDESTEKAVVQFALDNPAFGQVRVSNELRKQGIFV
                     SAGGFCLCRWFLSLQVVFVSAGGVRSIWLRHHLANFKQRLTALEKLVAEQGIILSEPQ
                     IQALERKKEDEIACGEIETAHPGYLGSQDTFYVGNIKGVGRIYQQTFIDTYSKVAFAK
                     LYTMKTAITAADMLNDKVLPFFESQRLPMLRVLTDRGSEYCGKVENHDYELYLAINDI
                     EHTKTKVKQPQTNGICERFHKTILQEFYQVAFRKRIYMDLEALQADLDEWLMYYNHHR
                     THQGKMCCGRTPMETLVDGKRIWAEKNLSSN"
     gene            3404..>4037
                     /locus_tag="PXD71_002346"
     CDS             3404..>4037
                     /locus_tag="PXD71_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDL6292414.1"
                     /translation="MYLMYVDESGSTTDPTQKYFVLSGIAVFERNTHWIEKKMNVIAS
                     QFCQYSEGSDPYFYELHGSPMRSGKSEWRGIPMSERINAIQDCLKLVQSNNIRIFAAV
                     IEQGYSSGQDTITECFEQISSRFDMYLSRLHAQGNTQRGISIFDKSSTEKSIQALART
                     FKNSGHTYGKLRNFSEVPLFLDSKSSRLIQLADLVSYAIFRHYEHNDSSFY"
BASE COUNT         1160 a          854 c          774 g         1249 t
ORIGIN      
        1 attagcaact gttttgtcca cttggcatag tttttgacgg atcagcaact attttgtcta
       61 ctatgccttt ttgttacttc cagtattttt tcttcgaggt ttgcccgatc cccggattaa
      121 agctgttggt tggatcaagc tgccggtaga atttgcgtaa ggtcggtttt gcttcgtaaa
      181 gatgaccgac attatgctcc gcagggtatt gtgccccacg tttatccagt aattctaaca
      241 tctgatgttc caacgccaaa caatctacac cttttttcac aatatagtct tgatggaaaa
      301 catggcacat aaaatgccca taataaagtt tgtgcgtgat ttgactctct atttccggcg
      361 gtagcacttc aaaccacgct ttatcatttc ggcgaagggc aatatctaaa gcaacaatat
      421 cttccacctc tttatcgtga atggctcgat agcgaatcgc tgccgaagca acggcaaaac
      481 gatgtaacat tgccgcttgg ctttcagtcg gattacactc caaataaccg cctttttgtg
      541 tatcagcaaa ataacgggca agatagtctc ttgcttcttg aacgcctttt ccacccattt
      601 tgataattaa gtgatgttca tactggttac ggtagtctcg aagactttcc ggcaaatgtt
      661 gcggtaacat ttttgagatt gcctgtaata ttttatcact gagatttgcc ggtaaaaagg
      721 tgagtttctt cgccattctg tccacttttg ctttaagtga aaagagttgt ggtaagcggt
      781 gtgtaccgag ttttttaatc acccaaaaag tatctttacc gtaatcagcg gcaatatcaa
      841 aggcatcacg atgaatatat tcaccggaaa tcggcaatac ctcaaagttg gctaacatat
      901 agcgacgaat atcgtttaat gtgtccgtat gatttgtccc gatataaaaa acagccgtgt
      961 ctttttcaag cggaaaggta tctaatcgca ccgcaaacac cgctaacttt cctgcactac
     1021 cggcagcctc gtagtggcgg gcaggatcgg cattaaaacg ggcaggggag tcttcatcaa
     1081 tttggcggac gtgattacaa taggcgtgat cgtgtccttt tccgcacgct tgctgaatat
     1141 ccttacgctg ataacgttgg gcttgcagat tttctaaaat ttcttccggc gtatcaccta
     1201 aatcaatacc taaatggttt tttaattcca gcgtgccgtc ttcatttaac tgggcaaaca
     1261 gtgccatttc agtataagcc ggcccacgtt gcactaatgc accccctgaa ttattacaaa
     1321 taccaccgat aaccgaagcc ccgatacagg acgatccgat caccgaatgc ggttcacgtc
     1381 cgtgcgtttt cagcaacaat tccagctcat ttaatgtcga gcccggtaag caaacgactt
     1441 gattggcatt gttaattaac tgaatgccgt tcattcgcat tgtgttaatg atcacaatat
     1501 cccgatcata atcgttgccg cttggtgttg aaccaccggt taatccggta ttggccgctt
     1561 gtgagataac aatgacatcg gcggcgacac aggcttttaa tacgttccag tattctaata
     1621 acgttgtagg ttttaccact gcaatcgcac tacccgtgcc aaagcgatac ccgctacgat
     1681 aggcttccgt cttagtcggg tcagtgatga tataagactg cccgacaatt tcagtaagct
     1741 gagaaatcag tgaattattt gacattatgc ttccctcaaa ggtagaaata gatagggcag
     1801 tatagcaata aacaaaagag agcaaagcag aatgttacaa aattgtgaac tactgcacag
     1861 aataaatgaa gaaatgaagt aaaagaaagc gagctaaaag agcgtcgccc gctttatttg
     1921 tagaaaatca aacctaatct gacagtcccc cgtttaaaat taccgtgtct gtcagattaa
     1981 tttgagctta aattcttttc tgcccaaatg cgttttccat caactaatgt ctccatcggt
     2041 gttcttccac agcacatttt tccttgatgg gttcgatgat ggttataata cattaaccat
     2101 tcatctaagt cagcttgcaa tgcctctaaa tccatataaa tccgttttct gaatgccact
     2161 tggtagaact cctgcaaaat cgttttgtga aaacgctcac agatgccgtt tgtttgtggc
     2221 tgtttcactt tggtttttgt gtgctctatg tcattgatag ccaaataaag ctcataatcg
     2281 tgattctcaa ctttcccaca atactcactg cctcggtctg tcagcactcg taacataggt
     2341 aacctctggc tttcaaagaa tggtaatact ttgtcattta gcatatcagc agcagtgata
     2401 gccgtcttca ttgtatacag ttttgcaaat gccactttac tataagtatc aataaacgtt
     2461 tgctgataaa tacgacccac acctttaata ttgccgacat aaaaggtatc ttgtgaccct
     2521 aaatagccgg ggtgtgccgt ttcaatttca ccacaggcaa tctcatcttc tttcttccgc
     2581 tccaaggctt gtatctgtgg ctcactcaga ataatgcctt gctctgccac caatttttcg
     2641 agggcagtta agcgttgctt aaaattagct aagtgatgtc tcaaccaaat ggaacgtaca
     2701 ccacctgcag agacaaaaac cacctgcaga gacaaaaacc acctgcagag acaaaaacca
     2761 cctgcagaga caaaaatccc ctgtttacgc agttcattac tcactcgaac ttgaccaaaa
     2821 gcaggattat ctaaagcgaa ttgtacaacc gctttttctg tggattcatc cacacgattt
     2881 ttaatgttgg gaacacgtcg attttgattt agtaatgcct caacaccgcc ttgttcgaca
     2941 gcctgttgat agcgataaaa agtatctcga ctcatcccca ttaccttaca ggcttgtgag
     3001 atattaccca gttcttctgc taaatttaat aaaccggtct tgtgtttaat aagtggattg
     3061 ctagaataaa acatgagagt ttcctttttg tttagattga tttttgacac tcatattcta
     3121 aacgggaaac tctcactttt tagagtgaat tgtcagatca agtctgatct tctacagacg
     3181 atccaggtct tacacattct ttcaagtttg taaagcctga aaattatttt ttatacttga
     3241 tttttgtcaa aaagtctcta cactatatgg cagggtcagt tttcgctgtt taacggtgtt
     3301 taatgagatt ttcattactg gctctcctta atagaaattt atggaagagc tatctgatag
     3361 agtaatctag cgatagctct ttttcattga acaaagaggc tatatgtatc ttatgtatgt
     3421 tgatgagtct ggttctacga cagatcctac tcaaaaatat tttgtattat caggtatcgc
     3481 tgtatttgaa cgaaatactc attggattga gaaaaaaatg aatgtgatag cctctcaatt
     3541 ctgtcaatat tctgaaggga gtgatccata tttctatgag ttacatggtt caccaatgag
     3601 atctggaaag agtgaatgga gaggcattcc aatgagtgag cgtattaatg ccattcaaga
     3661 ttgcttgaaa ttagttcaaa gtaataatat tcgtatattt gcagcagtta tagagcaagg
     3721 gtattctagt ggtcaggata ctattacaga atgttttgag caaatatcat ctcgatttga
     3781 tatgtatctg agccgtttac atgcccaagg taatacccaa agaggcattt ctatctttga
     3841 taaatcatcg actgagaagt caatccaagc acttgcaaga acattcaaaa atagtgggca
     3901 tacttatggg aaacttagaa atttctcaga agttccatta tttcttgact caaagtcatc
     3961 gagattaatt cagctagcgg atctagtttc ctatgcgata tttagacact atgaacacaa
     4021 tgatagctca ttttacg
//
LOCUS       DANLXS010000201         4008 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.204, whole genome shotgun sequence.
ACCESSION   DANLXS010000201 DANLXS010000000
VERSION     DANLXS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.204"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(111..1751)
                     /gene="pgi"
                     /locus_tag="PXD71_002347"
     CDS             complement(111..1751)
                     /gene="pgi"
                     /locus_tag="PXD71_002347"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL6292415.1"
                     /translation="MQKINPVQTFAWNALEQHKADNLSIPQLFAEDPNRFDKYSLRFE
                     DQLLVDFSKNAINEKTLTLLRQLANECGLESAKNAMFSGKKINRTENRAVLHVALRNR
                     ANTPIAVDGKDVMPEVNAVLAKMKDFCERVISGEWKGYTGKTITDVINIGIGGSDLGP
                     YMVTEALRPYKNHLNMHFVSNVDGTHIAEVLRKVNPETTLVLVASKTFTTQETMTNAL
                     SARKWLLDSAKDESAVAKHFVALSTNAKEVEKFGIDTANMFEFWDWVGGRYSLWSAIG
                     LSIALSLGFENFEQLLAGAHAMDKHFLITPIEKNIPTTLALVGIWNNNFLGAESEAIL
                     PYDQYMHRFAAYFQQGNMESNGKFVGRDGKPVTHQTGPIIWGEPGTNGQHAFYQLIHQ
                     GTKLIPCDFIAPAQTHNPLSDHHNKLLSNFFAQTEALAFGKSKETVEKEFLDAGKTLE
                     EVAEIVPFKVFSGNKPTNSILVQKITPYSLGALIAMYEHKIFVQGVLFNIYSFDQWGV
                     ELGKQLANRILPELETDEKINSHDSSTNGLINQFKAWR"
     gene            complement(1784..2032)
                     /locus_tag="PXD71_002348"
     CDS             complement(1784..2032)
                     /locus_tag="PXD71_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HDL6292416.1"
                     /translation="MLTPKNLEAIAQQLHNALPQSLKNVGNDLDEKFKQILQAQLARL
                     DVVTREEFDVQSQVLLRTREKLTELEKRLDELTQRTDE"
     regulatory      2156..2200
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2204..2872
                     /gene="queC"
                     /locus_tag="PXD71_002349"
     CDS             2204..2872
                     /gene="queC"
                     /locus_tag="PXD71_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDL6292417.1"
                     /translation="MTHQKPKAVVIFSGGQDSTTCLFQAIAEFGKENVEVVTFQYGQR
                     HSIELEKARWISNDLGVKQTLIDTSVIKAITTNALMDNNAEIQQQGSTPNTFVDGRNA
                     LFLLYTAIYAKGQGIQTIFTGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYNFNIRTP
                     LMYLTKKETWALADKLGAFDYIRDHTHTCYLGVEGGCHDCPSCVLREKGLNEYLAERE
                     VSNV"
     gene            2865..3287
                     /gene="queD"
                     /locus_tag="PXD71_002350"
     CDS             2865..3287
                     /gene="queD"
                     /locus_tag="PXD71_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDL6292418.1"
                     /translation="MFKIAKEFSFDMAHMLDGHDGKCQNLHGHTYKLQVEVAGELFQE
                     GAKRGMVMDYSDLKAVVKQHILEPMDHAFIYDLQSERESQVAQLLIDLNSKVYGIPTR
                     TTAEEMAKYMFEKLEKVGLPVSLIRLWETPTSYCEYSK"
     gene            3287..3913
                     /locus_tag="PXD71_002351"
     CDS             3287..3913
                     /locus_tag="PXD71_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDL6292419.1"
                     /translation="MAELISNPRFPIVEIFESLQGEGFNTGMPAIFVRFGKCNLTCPW
                     CDTNYNQFEQWTLSDILAKVRSYSAKNVIITGGEPTIQPNLSLLLEQFKKEGYFLAIE
                     TNGLKEVPAQIDYIATSPKRMYQEKYQRRCIEFANEVRIVVDGEVQGFCEQIESQIKA
                     EHYYLSPCEVEGKMNLLETITQIGLLNQRPNKPKWQLSIQTHKIVGIE"
BASE COUNT         1201 a          748 c          852 g         1207 t
ORIGIN      
        1 agaaaaaact gcttgtgtag aaatggcgct tagttcagtc ctcagctaaa attttagaaa
       61 aaaagcatag agatttttta cctctatgct ttttattgta ttgactatat ttaacgccaa
      121 gccttgaatt ggttaattaa accatttgtg gagctgtcgt ggctgttgat tttttcatcg
      181 gtttcgagtt cgggtaaaat gcggtttgca agctgtttgc ctaactccac gccccattgg
      241 tcgaaactgt aaatgttgaa cagcacgcct tgcacgaaga ttttgtgttc atacattgca
      301 attaatgccc ctaagctgta cggggtgatt ttttgcacca agatagagtt ggtcggtttg
      361 tttccgctga acactttaaa cggcacgatt tcagctactt cttccaacgt tttacctgca
      421 tctaaaaact ctttttcaac tgtttctttt gatttaccga aagctagtgc ttcagtttga
      481 gcaaagaagt tagaaagcag tttgttgtga tgatcgctta acggattgtg agtttgtgcc
      541 ggtgcgataa aatcacaagg aattaatttt gtgccttgat gaatgagttg atagaatgcg
      601 tgctgaccgt tagttcccgg ttcgccccaa ataatcggac ctgtttggtg agtaaccggt
      661 ttgccgtcac gccccacaaa tttaccgttt gattccatat tgccttgttg gaaataagcc
      721 gcaaagcggt gcatatattg gtcgtaaggt aaaatcgctt ctgattcagc acctaagaaa
      781 ttgttattcc aaatgccgac taaagctaac gtagttggaa tgtttttctc aatcggcgtg
      841 attaagaaat gcttatccat tgcgtgagca ccagcaagta gctgctcgaa gttctcaaaa
      901 cctaatgaaa gggcaatcga aagcccgatg gcagaccata atgagtaacg accgccaacc
      961 caatcccaga attcaaacat attagcggta tcaataccga atttttccac ttctttggca
     1021 ttggttgaaa gtgccacgaa atgttttgct accgcagatt catctttggc agaatcaagt
     1081 agccatttgc gagctgaaag ggcattggtc atcgtttctt gagtggtaaa ggtttttgaa
     1141 gccactaaca ctaacgtggt ttcaggatta actttacgca acacttcagc aatatgcgtg
     1201 ccgtccacgt tagacacaaa gtgcatatta aggtgatttt tataagggcg taatgcctct
     1261 gttaccatat aaggacctag atccgagcca ccaataccga tattgatcac atcagtaatc
     1321 gttttgccgg tatagccttt ccattcgccg gaaataacac gctcgcagaa atctttcatt
     1381 ttcgctaaca cggcattcac ttccggcatt acatctttgc catcgactgc gattggtgta
     1441 ttggcacggt tacgcagtgc aacgtgcaat acggcacggt tttcagtgcg gttgattttt
     1501 ttgccggaga acatcgcatt ttttgctgat tccaaaccac attcatttgc cagttggcga
     1561 agtaaggtta aggttttttc gttgatcgca ttttttgaaa aatcgactaa taattgatct
     1621 tcaaaacgga gggaatattt gtcgaaacgg tttggatctt cggcaaaaag ttgaggaata
     1681 gaaaggttgt ctgctttatg ttgctctaag gcattccaag caaaagtttg cacagggtta
     1741 attttttgca tctgagtttc cttatactaa attaaaaaat cgattattca tctgttcttt
     1801 gagtcagttc atcaagacgt ttttctaatt cggttaattt ttcacgagtg cggagtaaca
     1861 cttgagattg aacatcaaat tcttcacgag tgacaacatc aagacgggct aattgggctt
     1921 gaaggatttg cttaaatttt tcatctagat cattaccgac attttttaag ctttgcggta
     1981 aggcgttatg gagttgttga gcaatcgctt ctaagttctt cggcgtgagc atatttaaat
     2041 tccttactat aaatgagtat cccaatttta ttgaattttg ggtttaagag aaagcaaatt
     2101 ctttgtaaaa tatcaccttt cgcaaaatac aagcaaaaaa gaccgcttgt attttttatt
     2161 tgtggttcgt aaacctccca cacaaaaaaa ctaaggacac aaaatgactc atcaaaaacc
     2221 aaaagccgtt gtgatttttt ccggcggtca agactctacc acttgtttat tccaagctat
     2281 tgctgaattt ggcaaagaaa atgtggaagt cgttaccttt caatatgggc aacgccattc
     2341 gattgaactt gaaaaagcac gctggatttc taacgattta ggcgtcaaac aaacgctgat
     2401 tgatacttcg gtgattaaag cgattactac caatgcgtta atggataata atgctgaaat
     2461 tcaacaacaa ggcagcacac ccaatacctt tgtggatggg cgtaatgcct tatttctgct
     2521 ttataccgcc atttatgcca aagggcaggg cattcaaact atttttaccg gcgtgtgtga
     2581 gaccgatttt agtggttacc ctgattgccg agatgtattt gtaaaatcaa tgaatgtaac
     2641 gctaaattta gcgatggact ataatttcaa cattcgcacg ccattgatgt atttaactaa
     2701 aaaagaaact tgggcattag cagataagct gggtgctttt gattatattc gagaccatac
     2761 ccacacctgt tatttaggcg ttgaaggtgg ttgccacgac tgcccgagtt gcgtattgcg
     2821 agaaaaaggc ttgaatgaat atttagcaga aagggaggta agcaatgttt aaaattgcta
     2881 aagaatttag ttttgatatg gctcatatgc tagatggaca cgatggcaaa tgccaaaact
     2941 tacacggaca cacctacaaa ttgcaagttg aggtggcagg tgagctattt caagagggag
     3001 ctaaaagagg aatggtaatg gactattcgg atctaaaagc agtggtaaaa caacatattt
     3061 tagagccgat ggatcacgct tttatttacg acttacagag tgagcgtgaa agccaagtag
     3121 cacaattatt aattgattta aactccaagg tttatggtat cccaactcgt accacagccg
     3181 aagaaatggc gaaatatatg tttgaaaaat tagagaaagt gggcttaccg gttagcctaa
     3241 ttcgcttgtg ggaaacgcct acttcttatt gtgagtacag caaataatgg cggagttaat
     3301 ttcaaaccct cgttttccga ttgttgagat ctttgaaagc ttacaagggg aaggctttaa
     3361 taccggaatg ccggctattt ttgtacgttt tggcaaatgc aacctcactt gcccgtggtg
     3421 cgataccaat tacaatcaat ttgagcaatg gacgttaagc gatattcttg ccaaagtaag
     3481 aagctattct gcgaaaaatg tgattattac cggaggcgag ccaacgattc agcctaattt
     3541 atcgttatta ttagaacaat ttaaaaaaga gggatatttt ttagcgatcg aaaccaacgg
     3601 cttaaaagag gttccggcac aaattgatta tattgcgacc agcccaaagc gaatgtacca
     3661 agaaaagtat caacgccgtt gcattgaatt tgcgaatgaa gtccgtatcg tcgttgatgg
     3721 agaagtacag ggtttctgtg agcaaataga aagccaaatt aaagctgagc attactatct
     3781 ttcaccttgt gaggtagagg gaaaaatgaa cttgctggaa accatcaccc agattgggtt
     3841 attaaatcag cgtccaaata agcctaaatg gcagctaagt atccaaacgc ataaaattgt
     3901 ggggattgaa taagtagaag ttagaagatg atttgtaaaa gatcagactt gatctgacaa
     3961 ttcactctaa aaagtgagag tttcccgttt agaatatgag tgtccaaa
//
LOCUS       DANLXS010000202         4000 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.205, whole genome shotgun sequence.
ACCESSION   DANLXS010000202 DANLXS010000000
VERSION     DANLXS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.205"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(64..951)
                     /gene="galU"
                     /locus_tag="PXD71_002352"
     CDS             complement(64..951)
                     /gene="galU"
                     /locus_tag="PXD71_002352"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL6292420.1"
                     /translation="MKVIIPVAGLGTRMLPATKAIPKEMLILADKPLIQYIVNECVAA
                     GFKEIVLVTHSSKNAIENHFDTSFELETMLEKRVKRQLLDDVRSIVPKDVTLIHVRQG
                     QAKGLGHAVLCGRTVVGDEPFAVVLPDVLLGEFTANQKTENLAAMVKRFQETGYSQIM
                     VAPVPMENVSSYGVADCHGVDIPLGGAAPIAKMVEKPSIEEAPSNLAVVGRYVFSSNI
                     WNLLEKTPVGVGDEIQLTDAIDMLIHQETVEAFHMTGRSFDCGYKVGYMKAFVEYSLN
                     HDKCGKEFKAFIKELAKSL"
     gene            complement(1087..1272)
                     /gene="csrA"
                     /locus_tag="PXD71_002353"
     CDS             complement(1087..1272)
                     /gene="csrA"
                     /locus_tag="PXD71_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDL6292421.1"
                     /translation="MLILTRKIGEVLLVGDDVEITVLSIRGNQVKLGVNAPKEISVHR
                     REIYERIKASVEEENAE"
     gene            complement(1362..3989)
                     /gene="alaS"
                     /locus_tag="PXD71_002354"
     CDS             complement(1362..3989)
                     /gene="alaS"
                     /locus_tag="PXD71_002354"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL6292422.1"
                     /translation="MKTTSDIRQSFLEFFQTKGHTIVPSSSLVPENDPTLLFTNAGMN
                     QFKDVFLGLEKRPYSRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYF
                     KQDAIKFGWEFLTSPQWLGLPKEKLYVTVYETDDEAYDIWHKEVGVPSEHIIRIGDNK
                     GAPYASDNFWAMGDTGPCGPCTEIFYDHGPEHWGGLPGSPEEDGDRYIEVWNIVFMQF
                     NRLADGTMEKLPKPSVDTGMGLERMTAVMQHVNSNYETDIFQTLIKEVATLLNVKDLD
                     NKSLRVVADHIRACSYLIADGVLPSNEGRGYVLRRIIRRAVRHGNILGAKEAFFYKLV
                     PTLATVMGQAGEVLTQKQAHIQKTLKAEEEQFARTLERGLNLLEEALAKVENNTLSGE
                     VAFKLYDTYGFPLDLTADVCRERDIKIDEDGFNAEMTAQRERAKASSSFGTDYNNVIK
                     VDSKTCFSGYTETSLNDATVIGLFSNGKSVESIQSGENAVVILDKTPFYAEMGGQVGD
                     SGQISAEICNFEVVDTQKYGQVFGHIGQLTSGSLSVGDKVNTIVDRERRHAITLNHSA
                     THLLHSALRQVLGDHVAQKGSLVSENILRFDISQPEAITKSQLEEVERIVNAKVRENI
                     QVIIEEMDIESAKAKGAMALFGEKYGEVVRVVEMSDFSIELCGGTHVKQTGDIGLFKI
                     TSEGAVAAGVRRIEAVTGENAIAWLHTLQQAVQQSAELLKADSHSFVEKIVQLQEKAK
                     RTEKELQQLKDKLAAQAGSDLAKQATQINGVNVVIQQLENVEPKALRTMVDDLKNQLG
                     SAVVVFATTAEDKVNLIVGVTKDLTDKVNAGQLVGAMAEKVGGKGGGRPDMAMAGGSE
                     PQHLLQALSLAQEWITAKL"
BASE COUNT         1132 a          916 c          681 g         1271 t
ORIGIN      
        1 aaaagactgg atccaataga atatcagaca cagcccctta gatcatagtc taaactctaa
       61 ggcttagagt gatttagcta actctttaat aaatgcttta aattcttttc cgcatttgtc
      121 atgatttaaa ctatattcta caaatgcttt catatagccg actttataac cacaatcaaa
      181 tgagcgccct gtcatatgaa aagcttcaac cgtttcttgg tgaatcaaca tatcaattgc
      241 atccgtcagc tggatttcat cacctacgcc tactggcgtt ttttctaata aattccaaat
      301 atttgaggaa aatacataac gtcctactac cgctaaatta gaaggcgcct cttcaatact
      361 tggtttttca accattttcg caataggcgc tgcccccccg agagggatat ctacaccatg
      421 gcagtcagca accccatagc tacttacatt ttccattgga accggcgcaa ccataatttg
      481 gctatatccg gtttcttgga aacgcttcac catcgcagct aaattttccg ttttttggtt
      541 tgctgtaaat tcaccaagta atacatctgg taatacaacc gcaaacggtt catcgccaac
      601 cactgttcgt ccacatagaa ccgcatgacc taaacctttg gcttgccctt ggcgaacgtg
      661 aattaaggta acatcttttg gtacaataga acgtacatca tctaataatt gacgttttac
      721 tcgtttctcc agcatcgttt ccaattcaaa agaggtatca aaatggtttt cgatagcatt
      781 ctttgaagag tgagtaacca atacaatttc tttaaaacct gcagccacac attcatttac
      841 aatgtattga ataagaggtt tatctgcaag gataagcatt tcttttggaa tagctttggt
      901 tgcgggtaac atgcgcgttc ccaatccagc aacaggaata atgactttca tttggaatcc
      961 tttgatgact taacatatta gatataactg aaataaaccg cttagacata acaggcggtc
     1021 aattttcaga aaattttgca ttgcctgctt cgctgttatc atctagcagc tttacagtta
     1081 aaatgcttat tccgcatttt cctcttctac tgaggctttt attctttcat aaatctctcg
     1141 gcggtgtaca gagatttctt ttggtgcatt cacacctaat ttaacttgat ttcctcggat
     1201 gctgagtacc gtaatctcaa catcatctcc tacgagtaaa acttccccaa tcttacgagt
     1261 tagaataagc ataattgtct cctaaaaagt gaaaaactct ataaatactt aaatatagag
     1321 taaagtttta aaggcgagtt cagctcgcct tgttattatt tttaaagttt tgctgtaatc
     1381 cattcttgag caagtgacaa tgcttgcaat aaatgttgtg gttctgagcc tccggccatt
     1441 gccatatcag gacgtccgcc ccctttacct cccacttttt cagccattgc accaactaat
     1501 tgaccagcat tgactttatc ggttaaatct ttggtcacac ctacaatcaa attgacttta
     1561 tcttccgccg tagtcgcaaa tacaacaact gcactgccta attgattttt taggtcatca
     1621 accatcgttc tcaatgcttt tggttcaaca ttctctaatt gttgaatcac tacattcacg
     1681 ccattgattt gggtagcttg tttggctaaa tctgaacctg cttgtgctgc aagtttatct
     1741 tttagctgtt gaagctcttt ttctgtacgt ttagccttct cttgtaattg aacaattttc
     1801 tcaacgaatg aatgactgtc tgcttttaac aattcagcac tttgttgaac ggcttgttgt
     1861 aaggtatgca accaagcaat cgcattttca ccagtgacag cctctatacg gcgtacacct
     1921 gctgctaccg caccttcaga ggtaattttg aataagccaa tatcaccggt ttgtttaacg
     1981 tgtgtgccgc cacaaagttc aatcgagaaa tctgacattt ccactacacg cactacttcg
     2041 ccatattttt caccaaataa cgccattgca cctttcgctt ttgcagattc aatatccatt
     2101 tcttcaatga ttacctgaat attttcacgc actttagcat tcacgatacg ttcaacttct
     2161 tcaagttgtg atttagtaat tgcttccggc tgagaaatat caaaacgtaa aatattttct
     2221 gacactaatg agcctttttg agcaacgtgg tcgcctagca cttggcgtaa cgcagaatgc
     2281 agtaagtggg tagcactgtg gtttaatgta atagcatgac gacgctctct atctacgatc
     2341 gtattaactt tgtcgccaac agaaagtgag ccggaagtta attgcccaat atgcccgaat
     2401 acttgaccat atttttgcgt atctactacc tcaaaattgc aaatttctgc ggaaatttga
     2461 ccgctatctc ccacttgtcc gcccatttct gcatagaatg gggttttatc taaaatcact
     2521 accgcatttt cacccgattg aatagactca accgattttc cattactaaa taacccaatt
     2581 acagtagcat catttaagct ggtttcagta tatccactaa agcatgtttt actgtccact
     2641 ttaatcacat tgttataatc tgtgccaaag ctactgctgg ctttcgcacg ctcacgttgt
     2701 gcggtcattt ctgcattgaa gccgtcttcg tcaattttaa tatcacgttc acggcaaaca
     2761 tctgcggtta aatctaacgg gaagccataa gtatcataaa gtttgaacgc aacttcgcca
     2821 gataaggtgt tattttcaac cttcgctaat gcttcttcta ataaattcaa tccacgctca
     2881 agagtacggg caaattgctc ttcttctgct ttgagtgtct tttgaatatg cgcttgtttt
     2941 tgggtcagca cctcaccagc ttgccccatt accgttgcaa gtgttggcac caatttatag
     3001 aaaaaggctt cttttgcacc caaaatatta ccgtgacgaa ccgcccggcg gataatacgg
     3061 cgtaatacat agcctcttcc ttcatttgaa ggtaaaacac catcggcaat cagataggaa
     3121 caagcacgaa tatggtctgc cactacacgt aaagatttat tatctaaatc tttcacatta
     3181 agcaaagtag cgacttcttt aattaaagtt tggaaaatat ccgtttcgta gttggagttt
     3241 acgtgttgca ttaccgcagt catacgttct aaccccatcc ctgtatctac agaaggctta
     3301 ggcagttttt ccatcgtacc gtctgctaaa cgattgaact gcataaatac aatattccat
     3361 acctcaatat agcggtcgcc atcttcttct gggctacccg gtaagccacc ccaatgttca
     3421 ggcccgtgat catagaaaat ctcggtacaa gggccacaag ggccggtatc gcccatcgcc
     3481 cagaagttat ctgaagcata aggtgcgcct ttattatcgc caatgcgaat aatatgttcc
     3541 gatggtacac ctacttcttt atgccaaata tcgtaggctt catcatcagt ttcatacaca
     3601 gttacatata atttttcttt tggtaagcca agccattgtg gagaagttaa aaactcccaa
     3661 ccgaatttga ttgcatcttg cttgaagtaa tcaccaaagc tgaaattccc catcatttca
     3721 aaaaaagtat gatggcgagc ggtataacct acgttttcta aatcattgtg tttaccacct
     3781 gcacgtacac aacgttgtgc cgtagtcgct ctgctataag ggcgtttttc taacccaaga
     3841 aaaacatctt tgaattggtt catgcccgca ttggtaaata ataatgtcgg atcattctcg
     3901 ggaacaagag agctgcttgg aacaatcgta tgtcctttag tttggaaaaa ctctagaaaa
     3961 gattgtctga tatctgaagt tgttttcatt aaaaatcact
//
LOCUS       DANLXS010000203         3985 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.206, whole genome shotgun sequence.
ACCESSION   DANLXS010000203 DANLXS010000000
VERSION     DANLXS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.206"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..252
                     /locus_tag="PXD71_002355"
     CDS             <1..252
                     /locus_tag="PXD71_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292423.1"
                     /translation="EKISKNNKTQFSSTIRENQINFIRALLYMDYGIETPQEVKNAIN
                     SGELGRKIEKFRRENPNIENDKNFKFPTSVTLSNWYSKA"
     gene            330..581
                     /locus_tag="PXD71_002356"
     CDS             330..581
                     /locus_tag="PXD71_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6292424.1"
                     /translation="MQLQKTISTFTQPNHRGIYVMTYSEQSYLNVKDVCEMLQLNRST
                     IYRQIKQGIFPKPTKIGKSSRWKFADIKAFLEPKPQEAN"
     gene            581..961
                     /locus_tag="PXD71_002357"
     CDS             581..961
                     /locus_tag="PXD71_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6292425.1"
                     /translation="MAGLSIICPFCGNTARIRGSSRPSLLSYQAQLTCSNCGQLKADF
                     VGQLINIKRAVFIECDEANQWDKPENELIKEGKIQGKDNAQRLKELRGSQQDLFAKVP
                     GQLPPEKITPAEKIAKRANLSKTH"
     gene            1272..2333
                     /locus_tag="PXD71_002358"
     CDS             1272..2333
                     /locus_tag="PXD71_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDL6292426.1"
                     /translation="MKDLSPNQKQYTPFSAIMQKNLMDLLTKSARSPYSFSTVAKSTA
                     GRENPELHNTANSSTPLTRAIFIRSLHTPKERRNKACSSMVACSGQGSPLAVFGSPFV
                     AVFSPRYTLSPCTVRSVADSPLKLTNGLQKMNPSQITGKNRLNSFLFNNNQNDYSLLF
                     TNAKQSTPISAKISKNQDLCLTTLNRSPYASRAFAKSKAECGNSKEHQANNSTPLTRA
                     FFVRSSRTPKECQNKACSSMVACSGQGFALDCLPDVAVFHPVTRYRPTLWKMLAIAPK
                     NQHQELSKMKAFAYSFLCAIRTPETDTYQETVVRIISDSEQNARFQLTADYRLLLPRP
                     VAKIRLNSTAYTVKGGIYA"
     gene            2326..2490
                     /locus_tag="PXD71_002359"
     CDS             2326..2490
                     /locus_tag="PXD71_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292427.1"
                     /translation="MLNYSDIEQIRYFMDQAEEALDNNEEFKAVAELNTAQQFILALL
                     MNYEQGGENE"
     gene            2483..3118
                     /locus_tag="PXD71_002360"
     CDS             2483..3118
                     /locus_tag="PXD71_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292428.1"
                     /translation="MNNTTGQATQRNWQEIIANLQRLSESGESTIEIAKRYEAFSKTA
                     EQIQALVNNNGSEAEKEAFFAPVERFYLGLLETETRLKDAIAKDEENAQKLKEQKAAR
                     EAEQKRWDNLPFYAKDKFSDLICIESRETLEDKFFAISCMFDALKAVPNLLEIGLREE
                     GKDSVIDRLAEMSKLYLLFVDEQFSLLANEIQSGKYGKLDLNGSQNKGDLC"
     gene            3112..3330
                     /locus_tag="PXD71_002361"
     CDS             3112..3330
                     /locus_tag="PXD71_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292429.1"
                     /translation="MLTHRITLPDGTEIEFFAETDKETFAEIQRDLEGLKLPTFAEFV
                     AQERKKHRAKQAVKKARKIAKLARRKNR"
     gene            3472..3957
                     /locus_tag="PXD71_002362"
     CDS             3472..3957
                     /locus_tag="PXD71_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292430.1"
                     /translation="METALQLAQHLTGWTEEENSQAILKNFLDWKETFGEKTREETKI
                     VETLLDWINENEASFIEYPFNQEQRTPPKVAGYRILANNSTKTNEYFFIYPKQFKETL
                     AEFPLLMACTKLHEAGILHKPPRPEKGYEFQYTIAPKITGGSKKRAYKIVPIALEEEN
                     E"
BASE COUNT         1440 a          840 c          765 g          940 t
ORIGIN      
        1 gaaaaaatat caaagaataa caaaactcaa ttcagctcaa caatacgaga aaaccaaatc
       61 aattttatta gagccttgct ttatatggat tatgggatag aaacgcctca agaagtaaaa
      121 aacgccatta attcagggga actaggcaga aaaatagaga aatttaggag agaaaacccc
      181 aatatagaga acgataaaaa tttcaaattc cctacaagtg taacccttag caactggtat
      241 tcaaaagcct aaaatattgg aaatttccga atctactttg gaaatttcca aacttctaaa
      301 acctactcac ctcacaatct cccccaacaa tgcaactaca aaagacaata agcacattca
      361 cgcaaccaaa ccacaggggg atttatgtaa tgacctattc agaacaaagc tatttaaacg
      421 tgaaagatgt atgcgaaatg ctacaactca accgctcaac tatttatcga caaattaagc
      481 aagggatttt ccctaagcca actaagatcg gaaaatcaag ccggtggaaa tttgccgaca
      541 tcaaagcatt tttagagcca aaaccacagg aggcgaacta atggcagggc tttctattat
      601 ttgcccgttt tgtggcaata ccgcacgaat tagaggcagt tctcgcccta gccttttaag
      661 ttatcaagca cagctaactt gttcaaattg cggacaatta aaagccgact ttgtagggca
      721 gctaatcaac atcaaacggg ccgtatttat tgagtgtgat gaagcaaatc aatgggataa
      781 acccgaaaat gaactaatca aggagggaaa aattcaaggc aaagacaatg ctcaacgcct
      841 caaagaacta agaggctcac agcaagattt atttgcaaag gtgccggggc aactaccgcc
      901 cgagaagatc actccggcgg agaaaatcgc caagcgggca aacctttcaa aaacgcatta
      961 aacaggggga taaatggcaa caaaaaagcc ccgcaaggca aaccatacgg agctattaaa
     1021 atcgcataat gcgaaagata tgcaaagaaa ttaaagcagg cttgacgatt tctgaaggtg
     1081 ttagattctt tctaaagtct ttagggatag gtttatttct agcattgttt ttttattttt
     1141 tgccgtattt agcagaattt ataatcgccg taaaaaaaca actagcaaaa aaatcggctt
     1201 actttcaatg ataaaagcaa gccgaaatga caatccaaat ttttaacttt aaataaagag
     1261 tacaagaaga aatgaaagat ttatctccaa atcagaaaca atatacgcca ttttcggcta
     1321 taatgcaaaa aaatttaatg gatttattga caaaatccgc ccgttcgcct tattctttct
     1381 ctaccgttgc aaaatcaacg gcagggcgtg agaaccctga actacataat acggcgaaca
     1441 gtagcacgcc tttaacccgt gctattttta ttcgtagctt acacacacct aaagaacgcc
     1501 gaaataaggc gtgttcgtct atggtagcgt gtagcgggca aggttcgccc ttggctgttt
     1561 ttggttcgcc gtttgtagca gttttctcac cccgttacac gctatcgccc tgcaccgtga
     1621 gaagtgtagc cgatagtcct ttaaaattaa caaacggact acaaaaaatg aaccctagcc
     1681 aaattacagg caaaaaccgc ctaaactctt ttctatttaa caataatcaa aatgattatt
     1741 cgctattatt tactaatgca aaacaatcaa ccccgatttc tgctaaaata agcaaaaatc
     1801 aagatttatg cttgacaacc ctcaatcgtt cgccttatgc tagccgtgct tttgcaaaat
     1861 caaaagccga gtgtggaaac tcgaaagaac atcaggcgaa caatagcacg cctttaactc
     1921 gtgctttttt tgttcgtagc tcacgcacac ccaaagaatg ccaaaataag gcgtgttcgt
     1981 ctatggtagc gtgtagcggg caaggtttcg cccttgactg cttacctgat gttgcagttt
     2041 tccaccccgt tacacgctat cgccctacat tgtggaaaat gttagcgata gcccctaaaa
     2101 atcaacatca ggagctatca aaaatgaaag cattcgctta ttcattcctt tgtgctattc
     2161 gcacacccga aacagatact tatcaagaaa ccgtagtacg cattatttca gacagcgaac
     2221 aaaacgcccg ttttcagctt acagccgatt atcgcttact actcccccgc cctgttgcaa
     2281 aaatccgcct aaattctacc gcttacacgg tgaaaggggg gatttatgct taattattcc
     2341 gatattgaac aaatccgcta ttttatggat caagccgaag aggctttaga caataatgaa
     2401 gagttcaaag ccgtagcaga acttaacaca gcacaacaat ttattctggc tttattaatg
     2461 aactacgaac aagggggtga aaatgaataa tacaacagga caagctacgc aaagaaactg
     2521 gcaagaaatt atcgctaatt tgcagagact cagcgaatcc ggggaaagca caatcgaaat
     2581 cgctaaacgc tatgaggcat tttctaaaac cgccgagcaa atccaagccc tagtgaataa
     2641 caacggcagc gaagccgaaa aagaggcgtt ttttgcccct gttgagcgtt tttatcttgg
     2701 cttactagaa accgaaacac gcttaaaaga tgccatagcc aaagacgaag aaaacgccca
     2761 aaaattgaaa gagcaaaaag cagctagaga ggcagagcaa aaaagatggg acaacctacc
     2821 tttctacgcc aaagacaaat tcagtgattt aatttgcatt gaaagccgag aaacgcttga
     2881 agataaattc tttgcgatct cttgtatgtt cgatgcccta aaagccgtac ctaatctatt
     2941 agaaataggc ttgcgtgaag aaggcaaaga cagtgttata gaccgattag ccgaaatgag
     3001 caaactctat ttactgtttg tagatgagca attttcacta ctggcaaacg aaattcaaag
     3061 cggtaaatat ggcaagttag acctaaacgg ctcacagaat aagggggatt tatgctaacg
     3121 caccgcatta cactccctga cggcacagaa attgaatttt ttgcagaaac cgacaaagaa
     3181 acatttgcag aaattcaaag ggacttagag ggcttgaaat tgccaacctt tgccgagttt
     3241 gttgcccaag agcggaaaaa acaccgagca aaacaagcgg tcaaaaaagc ccgaaaaatt
     3301 gcaaaattag caagacggaa aaaccgctag cacctatcaa aaagacttat tattgcagaa
     3361 aatcacgctt taataaaaac gacctataaa gcaatccgca agaaatggtt aagtttaact
     3421 gaaaatatga gcggacaggt tcagcgggta gcgtgcgata gattcgcaat aatggaaacc
     3481 gccttgcagt tagcccaaca tttaacaggc tggacagagg aagagaacag ccaagcgatt
     3541 ttaaaaaact tcctagactg gaaagaaact ttcggcgaaa aaacaagaga agaaactaaa
     3601 atagtagaaa ccttgctaga ttggataaat gaaaatgagg cgagttttat cgagtaccca
     3661 ttcaaccaag agcaaagaac accgcctaaa gttgccggct atagaatact tgcgaataac
     3721 agcactaaaa caaatgagta ctttttcatt taccctaagc aatttaaaga aacgctggca
     3781 gaatttccgc tattgatggc ttgcaccaag ttacacgagg cagggatttt acataagcca
     3841 ccacgcccag aaaaaggcta tgaatttcag tacacaatag cccctaaaat aacaggaggg
     3901 agcaaaaaaa gagcttataa aatcgtaccg atagcattgg aagaagaaaa cgagtaatta
     3961 cagcaaaagc aagagtttca attct
//
LOCUS       DANLXS010000204         3976 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.207, whole genome shotgun sequence.
ACCESSION   DANLXS010000204 DANLXS010000000
VERSION     DANLXS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.207"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(360..1454)
                     /locus_tag="PXD71_002363"
     CDS             complement(360..1454)
                     /locus_tag="PXD71_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL6292431.1"
                     /translation="MKKTTLAILASALIANSALAITIYDHKETGTQIEFIGSARIKWE
                     STSDKTSPVNGKATRNHVNHAVDNNGSRFGFRLTQQVGHGFYALGRVEWRMRGDSSSQ
                     HNFDHIYDHQLYAGIGHKQYGELTYGNMTNIADEVKQTDLPNTLSLSDGLLTGSSRRT
                     LQYVYKGIEGLKLGAFYGGNSQRGNNGLDLVNKRKDIWGAAAIYNYKIDDNQSLKLAT
                     GATRERSEQSIGNYERIALAFGTAYTYGKTTLGLDLERRETEDQNAIGHKRIEKEVRT
                     VLLHRLTDQWNAYTMYAYKENKLNAVGLANDSKVKRNQFMLGTEYYIVPKHLKGFVEW
                     QATRAKHYTNDVKTAKSRNYTTVVGLRAYW"
     gene            1588..2766
                     /gene="lpxB"
                     /locus_tag="PXD71_002364"
     CDS             1588..2766
                     /gene="lpxB"
                     /locus_tag="PXD71_002364"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDL6292432.1"
                     /translation="MKQQFPVIGLVAGEVSGDILGAGLIKALKVHYPNARFIGVAGSR
                     MIEAGCQSLFDMEELSVMGLAEVVKHLPRLLKRRKQVIDEMLKLKPDVFIGIDAPDFN
                     LTVEEKLKASGIKTIHYVSPSVWAWRQKRVFKIARAANLVLAFLPFEKAFYDKFNVPC
                     RFIGHTMADAIPLEPNRTEACLALNIDESKRYMAILVGSRGSEIQFLAEPFLKAAQLL
                     KARFPDLQFLVPMVSQKRMEQFNAIKQQVAPELELNIIQGKARQAMIASECTLLASGT
                     AALEAMLCKSPMVVGYRMKPLTYWLAKKLVKTDYISLPNLLAQAPLVPELIQEACNPE
                     NLAQELSLFLADDEKNQQHKALLKQQFMQLHQSIQCNADEQAAQAVIDLLNGSTSGQI
                     "
     gene            2793..3845
                     /gene="sohB"
                     /locus_tag="PXD71_002365"
     CDS             2793..3845
                     /gene="sohB"
                     /locus_tag="PXD71_002365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDL6292433.1"
                     /translation="MWKEVLLNYGIFLLELLTVFGIIAVVVMLILESKKQNENGSISI
                     TNLSEKYKEQQKSLSGFFLSEEELKHQEKQEKKVAKEKAKAEKKRLKAGEECVESPSR
                     LFVLNFNGDMMAHEVNSLRREIDAVISLANPEKDEVLLKLESPGGVVHGYGLAASQLQ
                     RLRDRNIYLTAAVDKVAASGGYMMACVANKIVSAPFAVIGSVGVVAQVPNIHRLLKKH
                     DIDVDVMTAGEYKRTVTLVGENTEKGKQKFQQELEETHSLFKQFVARHRPQLDIEQVA
                     TGEHWFGQQAIELNLVDEIATSDDLLLNAIKEKEVIELKYKEKKKLAQKLGSQFEQSA
                     ENLLAKILHKSRSTLM"
BASE COUNT         1183 a          685 c          864 g         1244 t
ORIGIN      
        1 gacagacacg gtaatttaaa acgggggact gtcagattag gtttgatctt ctacatataa
       61 taacccctca tcactttttg cagtttaacc tcttttgcaa cagataattt aaatttaggc
      121 gttttggctg ctcagttatt gccattatga ttatattagt tcacactaaa attttagtgt
      181 tgtttcttaa agctgaagat gctaataggt gagtaagcgg tttttaccac tttgaagata
      241 aatgcaatag cttcttgttt gaaggattag ttgtatttaa tcataaaaaa tctgcacctt
      301 aatcagttgg ttagcccaac ttttaggtgc agatcattct agtacgcttt tcagttttat
      361 taccaatatg ctcgtaagcc tacaacagtc gtataattgc gagattttgc ggtttttaca
      421 tcattagtat agtgtttagc acgagttgct tgccattcaa caaagccttt taagtgtttt
      481 ggaacaatgt aatattccgt tcccaacata aattggtttc ttttaacttt gctatcattt
      541 gccaagccta cagcattaag tttgttttct ttataagcat acattgtgta agcattccat
      601 tgatcggtta aacgatggag taacacagtg cgaacttctt tttcgatacg tttgtgaccg
      661 atagcatttt gatcttccgt ttcacggcgt tctaaatcta agccaagggt agtttttccg
      721 taggtataag ctgtaccgaa ggcaagagca atgcgttcat aattgccgat agattgctca
      781 gaacgctcac gggttgcacc tgttgctaat tttaagcttt ggttatcatc aattttatag
      841 ttataaattg ctgctgcacc ccaaatatct ttacgtttat tgactaaatc taagccattg
      901 tttccacgtt ggctatttcc gccatagaat gctccgagtt ttaatccttc gatgcctttg
      961 taaacatatt gaagcgtacg tcgtgatgaa ccggtaagta agccatcact taagctgagt
     1021 gtatttggta agtcagtttg ttttacttca tcggcaatat tagtcatatt gccgtatgtt
     1081 aattcaccgt attgtttatg cccgattcct gcatagagtt gatgatcata aatatgatcg
     1141 aagttatgtt gtgagcttga atcgccacgc atacgccatt ccacacgacc taaagcatag
     1201 aatccgtgac ccacttgctg ggttaaacgg aaaccaaaac gagagccgtt gttatccact
     1261 gcgtgattaa cgtggttgcg ggtagcttta ccatttaccg gtgaggtttt atctgaggtg
     1321 ctttcccact taatacgagc agaaccaata aattcaattt gtgtaccggt ttctttatga
     1381 tcgtaaatcg taatagctag agctgagttt gcgattaagg ctgatgctaa aatcgctaat
     1441 gttgtttttt tcataaaaat cactccatat gaatagataa agggaggtag tgtatgtgaa
     1501 tattcattgc tagacaataa tgttttgtta gtttatatga tgattggtta taatagagtc
     1561 acttacagat cttcaataat atttaatatg aaacaacaat tccccgttat tggcttagtt
     1621 gctggtgagg tctctggcga tattcttggt gccgggttaa ttaaggcgtt aaaagtacat
     1681 tacccgaatg ctcgatttat cggtgttgca ggctctcgaa tgattgaggc tggctgccag
     1741 tctttgtttg atatggaaga gctttctgta atggggctag cagaggtggt taaacattta
     1801 cctcgtttac ttaagcgtcg taaacaggtt attgatgaga tgcttaaatt gaaaccagat
     1861 gtatttatcg gtattgatgc accggatttt aatttaaccg ttgaagaaaa actcaaagcg
     1921 agtgggatta aaacaatcca ttatgttagc ccttcggtgt gggcttggcg acaaaagcgg
     1981 gtgtttaaaa ttgccagagc agctaattta gtgttggcat ttctgccatt tgagaaagcg
     2041 ttctatgata aattcaatgt gccttgtcgt tttatcgggc atacgatggc agatgcgatc
     2101 ccactcgaac caaatcgtac agaggcatgt ttagcgttaa atattgatga atccaagcga
     2161 tatatggcaa ttttggtggg aagccgtggc agtgaaattc agtttttggc tgagcctttt
     2221 ttgaaggctg ctcagttgtt aaaagcccgt tttccggatt tacagttttt agtgccaatg
     2281 gtcagccaaa agcgaatgga gcaatttaat gcaattaaac agcaagtagc tcccgagttg
     2341 gaactgaata ttattcaagg caaggctcgt caggctatga ttgcgtcaga atgtactctt
     2401 ttggcatcgg gaacagcagc actagaggca atgttgtgca aatctccgat ggtagttggt
     2461 tatagaatga aaccgctgac ttattggtta gcaaaaaaat tagtgaaaac cgattatatc
     2521 tccttaccga atttattggc tcaagcccca ttggtaccag aattgatcca agaagcatgc
     2581 aacccggaaa atttagctca agagttatct ctatttttag ctgatgatga gaaaaaccaa
     2641 caacataaag cattgctaaa acagcagttt atgcagctac atcaatcaat tcaatgtaat
     2701 gctgatgagc aagctgccca agcggttatc gatctcctaa acggctcgac aagcggtcaa
     2761 atttagttag ttttttgtaa aaaggaaaat ttatgtggaa agaagtatta cttaattatg
     2821 gtattttttt attagagtta ttaactgtat ttggcattat tgcagtggtg gtgatgctga
     2881 ttttggaatc gaaaaagcag aatgaaaacg gctcaatttc aattaccaat ttatctgaga
     2941 aatataaaga gcagcaaaaa tctctttccg gcttttttct aagtgaggaa gaattaaaac
     3001 atcaggaaaa gcaggagaaa aaagtagcaa aagagaaagc taaagcagaa aaaaaacgct
     3061 taaaagcagg cgaagaatgt gtagaaagcc cttcccgttt atttgtattg aattttaatg
     3121 gcgatatgat ggctcacgaa gtaaattccc tacgccgtga aattgatgcg gtaattagcc
     3181 tcgcgaatcc ggaaaaagat gaagttttac ttaaacttga aagtccgggc ggagtggtgc
     3241 acggttatgg attggctgct tcgcagttac aacgtttgcg tgatcgcaat atttatttaa
     3301 ccgcagcggt agataaagta gctgccagcg gtggttatat gatggcgtgt gtggcgaata
     3361 aaattgtctc tgccccattt gcggtgattg gctcagtggg ggtggtggct caggtgccga
     3421 atatccaccg cttactgaaa aaacacgata ttgatgtgga tgtgatgact gcaggtgaat
     3481 ataaacgcac ggtgacgctg gtgggagaaa ataccgaaaa agggaaacag aaattccagc
     3541 aagaactgga agaaacgcac tctctgttta agcaatttgt ggctcgacac cgcccacaac
     3601 tggatattga gcaggttgca acaggcgagc attggtttgg tcagcaagcg attgagctaa
     3661 atttggttga tgagattgca accagtgatg atctgctcct caatgcaatt aaggaaaaag
     3721 aagtgatcga gctaaaatat aaagagaaga aaaaacttgc tcagaaattg ggttctcaat
     3781 tcgagcaatc agcagagaac ctgctcgcta agattttaca taaaagtcgt tcaacgctga
     3841 tgtagcgtat atattattaa agggctatcg tttatatagc cccttatttt atcagttagt
     3901 aatagaatga agtatttgtt cactatttaa atgacctgga atacttttct ttaaattgag
     3961 tgaagaatct aacaat
//
LOCUS       DANLXS010000205         3963 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.208, whole genome shotgun sequence.
ACCESSION   DANLXS010000205 DANLXS010000000
VERSION     DANLXS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.208"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(129..836)
                     /gene="gloB"
                     /locus_tag="PXD71_002366"
     CDS             complement(129..836)
                     /gene="gloB"
                     /locus_tag="PXD71_002366"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDL6292434.1"
                     /translation="MLQITPIPALKDNYIWAIRNGQDVIIIDPSEHQPALNFIAKNAL
                     NLTAILITHNHHDHTDGVSGIVEQYPNIPVYGPQEVAEFANVIVSPEQHISLFGYDVR
                     IIESGGHTAGHISYLFGYDYLFCGDALFSGGCGRVFTGDYQAQFDALQRFKNLPNSVK
                     AYPAHEYTKSNLKFAEAVLPPSCVLSEYQEQVDLLRSQNKPTLPTTIGLEMQVNPFMQ
                     AVDLAEFIALRQQKDNF"
     gene            complement(871..968)
                     /gene="ffs"
                     /locus_tag="PXD71_002367"
     ncRNA           complement(871..968)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PXD71_002367"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1128..2129)
                     /gene="bioB"
                     /locus_tag="PXD71_002368"
     CDS             complement(1128..2129)
                     /gene="bioB"
                     /locus_tag="PXD71_002368"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDL6292435.1"
                     /translation="MTTFQLRLKAKDELTPHPTAQYWRKCQVEGLFDLPFMDLVFRAA
                     QVHRENFDANAIQLSTLLSIKTGGCPEDCEYCPQSARYDTGIEKQTMLDVETIVEKAK
                     IAKERGASRFCMGAAWRGPRPKDIKVVSEIINAVKSLGLETCGTFGMLEEGMAEELKQ
                     AGLDYYNHNLDTDPERYNKIIHTRNHEDRMDTLGKVRNAGLKVCCGGIVGMNETRPER
                     AGLIASLANLDPQPESVPINQLIKVEGTPLTNAEDLDWTEFVRTIAVARITMPQSFVR
                     LSAGRTTMPEAMQALCFMAGANSIFYGDKLLTTENPEEDHDRQLMDKLDLYPLQYIA"
     gene            complement(2222..3829)
                     /gene="pckA"
                     /locus_tag="PXD71_002369"
     CDS             complement(2222..3829)
                     /gene="pckA"
                     /locus_tag="PXD71_002369"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL6292436.1"
                     /translation="MLNRIEQELADLGITNVKEIVRNPSYEQLFEEEMKSDLKGYEKG
                     VLTNLGAVAVDTGIFTGRSPKDKYIVLDETTKDTVWWTSDAAKNDNKPMTQSTWQSLK
                     TLVTDELSNKRLFIIDAFCGASPEHRINVRIITQVAWQAHFVKNMFIRPTEEELKDFK
                     PDFVVMNGSQVTNPNWKEQGLNSENFVAFNITEGVQIIGGTWYGGEMKKGMFSMMNYF
                     LPLKGVASMHCSANVGEEGDVAVFFGLSGTGKTTLSTDPKRKLIGDDEHGWDENGVFN
                     YEGGCYAKTINLSIENEPDIYRAIRRDALLENVVVREDGTIDFDDKSKTENTRVSYPI
                     YHIDNIAVPSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERG
                     ITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDT
                     RGIIDAILDGSIEKAEMSTLPIFNLAIPTALPGVETHILDPRNTYEDKAQWQAKAEDL
                     AGRFVKNFEKYATNEEGKALIAAGPKL"
BASE COUNT         1119 a          849 c          760 g         1235 t
ORIGIN      
        1 gctaaaaata gtcgtaacta taccatagga taagccagag tggaattgac aagcgatgtt
       61 tgcaaaggtt ttatttaacc aaaaagcatt aaagtgagag gctttaatgc ttaattagaa
      121 atatgaggtt aaaaattgtc tttttgctga cgaagtgcaa taaattcggc taaatcgacc
      181 gcttgcataa acgggttcac ctgcatttcc aagccaatag tggttggcaa agtcggtttg
      241 ttttgcgaac gtaaaagatc aacctgttct tgatattccg ataatacaca gcttggtggt
      301 agaaccgctt ctgcaaattt caaattgctc ttggtgtatt cgtgagccgg gtaggcttta
      361 accgagttag gcagattttt aaagcgttgt aacgcatcaa attgggcttg gtaatcaccg
      421 gtgaaaaccc gcccgcaacc gccggagaat aacgcatcgc cacaaaacag ataatcatag
      481 ccaaataaat aactgatatg ccctgcggta tgcccaccac tttcaataat ccttacatca
      541 taaccaaaca ggctgatatg ttgttcaggc gagacaatca cattggcaaa ttcagccact
      601 tcttgcggac cataaacagg gatattcgga tattgctcaa caataccact caccccatca
      661 gtatggtcgt ggtgattatg ggtaatcaag atagcggtta aatttaacgc attttttgca
      721 ataaagttta atgctggctg atgttctgat ggatcaataa taatcacatc ttgcccgtta
      781 cggattgccc aaatgtaatt atcttttaat gccggaatgg gggtgatctg gagcataacg
      841 ttctcctgat agaactgaaa aaagggtggg gcattaccag cctccctcgg cacacggaaa
      901 tttcaaccca gactgctttc ttccgaacct gatcaagtaa gctgaacacc attgcgagga
      961 accaataacg ccccatcgaa ttacgcaagc ttgcgtaagg cctcacatta tagataaatt
     1021 taaaaataag acaagaacga attttgcaag cggttatttt atgttagaaa tttgcgtaaa
     1081 ataacgattg attctttaat tttcattcag gcgaaaatca ttggcactca ggctatatat
     1141 tgtagcggat ataaatctaa tttatccatt aattggcgat catgatcttc ttccggattt
     1201 tctgtagtaa gtaatttatc gccatagaaa atagaatttg ctcctgccat aaaacagagg
     1261 gcttgcattg cctccggcat agtagttctg ccagcagaaa gtcggacaaa gctttgaggc
     1321 atagtaattc ttgctaccgc aatggtacga acaaattctg tccagtctaa atcttcggca
     1381 ttggtaagcg gtgtaccttc tactttaatc aactgattaa tcggtacgct ttcaggttga
     1441 ggatcgaggt tagcgaggct tgcaattagg cctgctcgct cggggcgggt ttcgttcatt
     1501 cccacaatac cgccacagca aactttcaag ccggcattgc gaactttacc taaagtgtcc
     1561 attcgatctt cgtgatttcg ggtatggata attttgttat agcgttcagg gtcggtatct
     1621 aaattatggt tgtagtaatc cagccctgct tgctttaatt cttctgccat tccctcttca
     1681 agcataccga aagtaccaca ggtttctagc cccaatgatt tcaccgcatt gatgatttct
     1741 gagactactt taatatcttt aggcctaggt cctcgccacg cagcccccat acaaaagcgg
     1801 cttgctccac gctctttggc aattttagct ttttctacaa tcgtctctac atcaagcatt
     1861 gtttgctttt ctattcccgt atcatagcgg gcagattggg ggcaatattc gcaatcttcc
     1921 ggacagcctc ctgttttaat tgagagtaac gttgaaagct ggatcgcatt agcatcaaaa
     1981 ttttcacggt gaacttgtgc cgctctaaac actaaatcca taaacggcag atcaaatagt
     2041 ccttccactt ggcattttcg ccaatattgt gctgttgggt gtggggtaag ctcatctttg
     2101 gctttcagcc ttaattggaa tgttgtcatt attgtacttc tcctttatac aaaaggacta
     2161 aacctcattc aaggtttagt cctaaactaa ttttttagcg attataactt ataattacaa
     2221 cttataactt cggaccagca gcaattaatg ctttgccttc ctcgttggtc gcatattttt
     2281 caaagttttt cacaaatctg ccagctaaat cttctgcttt agcttgccat tgtgctttgt
     2341 cttcataagt gttacgaggg tctaaaatgt gggtttctac acccggtaac gcagttggaa
     2401 ttgctaaatt gaagattggt aatgtactca tttccgcttt ctcaattgag ccgtctaaga
     2461 tggcatcaat aataccccgt gtatctttaa tcgaaatacg tttacctgtg ccgttccaac
     2521 cggtattcac taaataggct tccgcaccgg ctgcttccat acgtttcact aacacttcag
     2581 catattgtgt cgggtggagt gataagaacg ccgcaccgaa acaagcagag aaagttgggg
     2641 ttggctcagt aataccacgc tctgtacctg ctaattttgc ggtaaagcct gataagaagt
     2701 agtatttagt ttgttcaggc gttaatttag acaccggtgg caatacgccg aatgcatctg
     2761 ccgttaagaa aatcactttt ttagcgtgtc ctgcttttga cggcacggca atattatcaa
     2821 tatgataaat cggatagctc acacgggtat tttctgtttt tgatttatca tcaaaatcaa
     2881 tagtgccatc ttcacgcaca accacgtttt ctaataacgc atcacggcgg attgcacgat
     2941 aaatatccgg ctcgttttca atagaaaggt tgatggtttt cgcatagcaa ccaccttcat
     3001 agttaaatac gccgttttcg tcccaaccgt gttcatcgtc accaatcaat ttgcgtttcg
     3061 gatcggttga tagtgtcgtt ttaccggttc ctgataaacc aaagaaaacc gccacatcac
     3121 cttcttcgcc cacattggct gaacagtgca ttgaagcgac accttttaat ggtaagaagt
     3181 agttcatcat agagaacata ccttttttca tctcaccacc gtaccaagta ccgccaatga
     3241 tctgaacacc ttctgtgata ttaaatgcca caaagttttc tgaatttaag ccttgctctt
     3301 tccagttcgg gttagttact tgggaaccgt tcattaccac aaaatccggt ttgaaatctt
     3361 ttaattcttc ttctgttgga cgaataaaca tatttttcac aaagtgagct tgccacgcaa
     3421 cttgggtaat aatacgcaca ttgatgcgat gctcagggct tgcaccacag aaagcatcaa
     3481 taataaataa acgcttatta gataactcat cggtcactaa ggtttttaaa ctttgccaag
     3541 tagattgagt catcggttta ttatcatttt tagctgcatc agatgtccac cagacggtat
     3601 cttttgtcgt ttcatctaat acgatatatt tatctttcgg cgaacgcccc gtgaaaatgc
     3661 cggtatctac cgctactgcg cctaaatttg ttaggacgcc tttttcataa cctttaagat
     3721 ccgatttcat ctcttcttca aacagttgct catagctagg gttacgcacg atttctttta
     3781 catttgtaat gcctaagtct gcaagttctt gttcaatacg atttaacata gtggttacct
     3841 cataatagtt ataaatttaa aactgggttg attatacgaa aattgaaaaa taaaaaatgt
     3901 tacatacttc aaatttttga catttagttt aaaaaaatcg ctttaaaata cgcatttgtt
     3961 tgt
//
LOCUS       DANLXS010000206         3958 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.209, whole genome shotgun sequence.
ACCESSION   DANLXS010000206 DANLXS010000000
VERSION     DANLXS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.209"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            105..1307
                     /gene="yedE"
                     /locus_tag="PXD71_002370"
                     /pseudo
     CDS             105..1307
                     /gene="yedE"
                     /locus_tag="PXD71_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
     gene            1343..1612
                     /gene="yedF"
                     /locus_tag="PXD71_002371"
     CDS             1343..1612
                     /gene="yedF"
                     /locus_tag="PXD71_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDL6292437.1"
                     /translation="MAFTVVQKLDKDPKDITPDYTLDTLGEPCPYPAITMLETMPQLQ
                     KGEILELLSDCSQSINNIPIDTKNHGYTLLSVEQNGTKLRYLIQR"
     gene            1712..2056
                     /locus_tag="PXD71_002372"
     CDS             1712..2056
                     /locus_tag="PXD71_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL6292438.1"
                     /translation="MNKYALLAIFWFIIGIYALLFRESTNDIPPFPHFDKVSHFALFF
                     IQIWLSANAFIRSKKGIPYFGLIIFALLYAFTSEWAQATFTSTREGSWLDGLADMLGA
                     SLSLLLVKYCKK"
     gene            complement(2119..3066)
                     /locus_tag="PXD71_002373"
     CDS             complement(2119..3066)
                     /locus_tag="PXD71_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL6292439.1"
                     /translation="MNRITQILNIKHPIVQAPMSWLTDAHLVASVAEAGGLGFLAPHA
                     GQTTNPTSNEEVLDRMRNEIRKVKALTDKPFGVPFILSYDFSLIPLMVDLFIEERVPV
                     VLDNGWLDPEIYAKFKAAGIKIICRLPNPNLADALKAQALGADILVLTGFDEGGTLPM
                     KEVGSFNVLAEFVGKVDLPIMLAGGIADKKAVQAALTLGAEGVWIGTAFIATEECRAS
                     EKVKNWIVEANANDLLLFRTEPHYYRSLPTGLAKKCFEMSENGATRSEIAKVMNAGTG
                     MRLGMLEGDFDNGYVSVGNGISQINKIKSVKELIDELMA"
     gene            3249..>3958
                     /locus_tag="PXD71_002374"
     CDS             3249..>3958
                     /locus_tag="PXD71_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292440.1"
                     /translation="MSIRWENIKPLQGSQNSAFEELVCQLARQEFKSQGKFTRISAPD
                     GGIEAMCELPDGSVCGWQAKYFLSSFSSSQWQQIEDSFKESLKNYPKLTKYYVCVATD
                     RANANVSGKKSFLTKWEEYTQRWKDFAQSQGREIEFEFWGSFELSDLLSKPENAGKKF
                     FWFNESELSDKWFEQYNQQAINNLGVRYTPEINVDLPIAMQLESLARTQIFKKHLSDE
                     FHKLLVEIKSQYKSLYRY"
BASE COUNT         1151 a          828 c          778 g         1201 t
ORIGIN      
        1 ttttgtgtat aggaaatgaa ctaattactg tgtcaatcag tccctttctt catcgttata
       61 ccctcttgca aatccgatta atctatcctt agttaaggtg ttttatgtta gcgatttggc
      121 aaagttttaa acaaaattat ctaatcaaat attggaatcc tgttgcagca gtgatcgcag
      181 ctggtattat ttcagcttat tatttcggca ttactggcac ttattgggcg gttacagggg
      241 aatttacccg ttggggcggt catctgttaa atgctttggg tgttgatgtt tcgaactgga
      301 gctattttca actcatcggc ttagacggca ccatctttac ccgcattgac ggtgtgatga
      361 ttctcggtat gtttgccggc tgtctttctg ccgcattatg ggcgaacaac gtcaaatggc
      421 ataatcagcc acataaaaaa cggattatcc aagccttgat tggcggtgcg ttagccggtt
      481 tcggagcccg tttagcaatg ggctgtaacc tcgcctctct ttttaccggt attccgcaat
      541 tctccgtcca cgcttggttt ttcacctttg cgaccgccat cggcacttac tttggggtaa
      601 aagtgacttt attaccgatg tttagagtga aattagagct gaaaaaaggg gcagcaaaaa
      661 tcaaagaaaa cgatccgaaa caagctaatc gccgtttttg ggttggtatg ctcattttta
      721 tcacttatct ttctgcttcc ctttatgtaa tgcaatcctc aatcaaactt ggttttgcaa
      781 tgctttgcgg cttagccttt ggcttattga ttgaacgagc ccaaatttgc tttacctccg
      841 ctttccgtga cttatggatt accggacggg cttatatggc aaaagcaatt attttcggca
      901 ttattgccgg cacaatcggc gtgttctctt atattcaatt aggtgtttca cctaaaattt
      961 tgtgggctgg cccaaatgcc attatcggcg gattattatt cggatttggc attgtgcttg
     1021 ccggcggctg tgaaaccggc tggatgtacc gttcaatgga aggacaggtt cattttatgt
     1081 gggtcggcgt aggcaatgta gtcggttcaa ccctgctggc ttactactga ggcgacttag
     1141 ctccggtgct tacccttgat tatgaaaaaa tcaacctact caaagaattt gggccggttg
     1201 gcggattatt catcaattat ggcttattaa ttctttgctt aatcgctgta gtttggtggg
     1261 aaaaacgctt ccttgccaaa gcgaaagcaa aattagcaac tgcgtaatct ttttaacttc
     1321 aaccaacaag aggaaaatga ctatggcatt caccgttgta caaaaactag ataaagatcc
     1381 aaaagacatc acgcctgatt acaccttaga taccttaggc gaaccttgcc cttaccctgc
     1441 gattacgatg ttagaaacaa tgccacagct gcaaaaagga gagatattgg aactgttaag
     1501 cgattgttcc caatcgatta ataatattcc gattgatact aaaaatcacg gctataccct
     1561 attaagcgtg gaacaaaacg gcactaaatt acgctattta attcagcgtt aattgcaaaa
     1621 aattcgcaca aaacgaccgc ttgtcgtaaa atagcaacta tttaagagcc ttgtaacgca
     1681 aggctctttt attctaagag gtaaaagaaa aatgaataaa tacgctttac ttgcaatatt
     1741 ttggtttatt attggcattt atgctttact ttttcgtgaa agcactaatg atataccgcc
     1801 ttttccacat tttgataaag taagccattt tgccttattc tttatccaga tctggttatc
     1861 tgcaaacgcc tttatccgtt caaagaaagg gataccttat tttgggctaa taatttttgc
     1921 cttactctat gctttcacca gcgaatgggc tcaggcaacc ttcacctcaa cccgagaagg
     1981 ttcttggctt gatgggctag cagatatgct aggagcctca ctttcattat tgctggtaaa
     2041 atattgcaaa aaataaagca taaaagaccg cttgttatat ggtttagcca tactacaagc
     2101 ggtcattttt taccaaaatt atgccatcaa ctcatcaatc aattctttca cacttttgat
     2161 tttgttgatt tgtgaaatgc cattgcccac cgacacatag ccattgtcaa aatccccttc
     2221 taacataccc aaacgcattc ctgtaccagc gttcattacc ttagcaatct cactacgagt
     2281 tgccccattt tcgctcattt caaagcattt tttcgctaat ccagtcggca gtgaacggta
     2341 gtaatgtggt tcggtacgga ataacagcag atcattggca tttgcctcca caatccagtt
     2401 tttcactttt tcgcttgcac ggcattcttc ggtggcgata aatgccgtgc caatccacac
     2461 gccttcagcc ccaagcgtta aggcagcctg caccgctttt ttatcggcaa taccaccagc
     2521 cagcattatc ggcaaatcca ccttgcccac aaattccgcc agcacattaa aactgcctac
     2581 ctctttcatc ggcaaagtac cgccctcatc aaaacccgtc agcaccaaaa tatctgctcc
     2641 caatgcttgg gcttttaaag cgtccgccaa attgggatta ggcaaacggc agatgatttt
     2701 aatgccagca gctttgaatt tggcgtaaat ctctgggtca agccagccgt tatccagcac
     2761 cacaggcaca cgctcttcaa tgaataaatc caccattaac ggaatcagcg aaaaatcata
     2821 agacaaaata aatggtacgc caaaaggctt atcggtcaat gccttaacct tacgaatctc
     2881 gttacgcata cggtcaagta cttcttcgtt ggaagttgga ttggtggttt gccctgcgtg
     2941 tggggctaaa aaaccaagcc caccagcctc ggcaacgctt gctaccagat gagcgtccgt
     3001 cagccaagac attggcgctt ggacgatagg gtgcttgata tttaagattt gggtaatgcg
     3061 gttcattgga ttttcctgtt tgaataaaaa gaattggacg tagtttaatg gttttggatt
     3121 tgagagggaa tgcgtgaaaa tgggaaggtg gttttcggat ttggaaaatt atcaatgcta
     3181 aaatacttga aatacacacg ccaatcctca tatcaacaaa attgaatatt taattatggt
     3241 aaaagaaaat gtctattaga tgggaaaaca tcaagccctt acaaggttct caaaatagtg
     3301 cttttgaaga gctagtatgc caattggcac gccaagaatt taaatctcaa ggaaaattta
     3361 ctcgaatttc tgcacctgat ggtgggattg aagcaatgtg tgaattgcca gatggttccg
     3421 tatgtggttg gcaagccaaa tattttttaa gctcattttc ttcatcacaa tggcaacaaa
     3481 ttgaagattc ctttaaagaa tctttaaaaa actatcctaa actaaccaaa tactatgttt
     3541 gtgtggcaac cgatagagca aatgccaatg tatctggaaa gaaatcattt cttaccaaat
     3601 gggaggagta tacccaaaga tggaaagact ttgctcaatc acaaggtcgt gaaatagaat
     3661 ttgaattttg gggaagtttt gaattatctg acttattatc aaaacctgaa aatgcaggca
     3721 aaaaattctt ttggtttaac gaaagcgaat tatctgacaa atggtttgaa caatacaatc
     3781 aacaagccat taataatctt ggtgtgcgtt atacacctga aattaatgtt gatttaccta
     3841 ttgcaatgca attagaaagc ttggcaagaa cccaaatttt caaaaaacat ctgagtgatg
     3901 aatttcataa attgttagta gaaattaaat cacaatacaa atctctttat cgttacga
//
LOCUS       DANLXS010000207         3916 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.210, whole genome shotgun sequence.
ACCESSION   DANLXS010000207 DANLXS010000000
VERSION     DANLXS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.210"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(25..1564)
                     /locus_tag="PXD71_002375"
     rRNA            complement(25..1564)
                     /locus_tag="PXD71_002375"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1918..3879)
                     /gene="recD"
                     /locus_tag="PXD71_002376"
     CDS             complement(1918..3879)
                     /gene="recD"
                     /locus_tag="PXD71_002376"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDL6292441.1"
                     /translation="MLNLLLELKQEKAISDLNYQFARFIDNKQKPYGYSPLEQDLAVL
                     AAALVSYQVGLGHTALRLENLSLSSLFELNSKPQTYHLLEQILQKIEQIPPLEWQSVL
                     KNHIAFSYSPETIKPMLFQNSLLYFYRYWQAEHRIAGYLQQAVDFSEKFANTELNQSI
                     LNQLFTGKAQTIDWQKIAVATALEKQFSLISGGPGTGKTTTVVKLLLGLQLKQQALQK
                     PYLQIALAAPTGKAAARMKESLESKLAEEKGLSEELKRSIPTEAVTIHRLLGARPLTE
                     KTKYHTKNPWHYDLVVLDEASMIDLSMMEKVVQALKPNSRLIMLGDKDQLASVEAGSI
                     MGELGSFIDNGYSQSHCDYLKAVTDYQIEAGNALPIGDCLSHLKHSYRFGEKAWIGEL
                     ANAVNDQKINLSWEIFDKYKNSDKLENFCYPETKETTDKSSWIEKSVQMVVDKAVELY
                     RDYLQAVQKREKNPDSVSVKAIFEKFQKVRILSALRVSELGVERLNMAIAEGLRKASL
                     VKFNHSRDRYIGKPILMTENMPINSIASGDVGIILPDENGESRVYFDSLQENREHHSL
                     PLGRVSNYEAAYIMTVHKSQGSEFEHTVLVLPHSISPVLTKELIYTAITRAKDKFTLF
                     GSEKVWKYSVGAKTERQSGLKEQIRGKFS"
BASE COUNT         1032 a          990 c          729 g         1165 t
ORIGIN      
        1 acttgctgtc gttctcgatt ttggtaagga ggtgatccaa ccgcaggttc ccctacggtt
       61 accttgttac gacttcaccc cagtcatgaa tcataccgtg gtaaacgccc ccctttcggt
      121 taagctatct acttctggta caacccactc ccatggtgtg acgggcggtg tgtacaaggc
      181 ccgggaacgt attcaccgca acattctgat ttgcgattac tagcgattcc gacttcatgg
      241 agtcgagttg cagactccaa tccggactta gacgtacttt ctgagattcg ctccccatcg
      301 ctggctcgcc gccctctgta tacgccattg tagcacgtgt gtagccctac tcgtaagggc
      361 catgatgact tgacgtcatc cccaccttcc tccggtttat caccggcagt ctcctttgag
      421 ttcccgacct aatcgctggc aacaaaggat aagggttgcg ctcgttgcgg gacttaaccc
      481 aacatttcac aacacgagct gacgacagcc atgcagcacc tgtctcatgg ttcccgaagg
      541 cactctcata tctctacaag attccatgga tgtcaagagt aggtaaggtt cttcgcgttg
      601 catcgaatta aaccacatgc tccaccgctt gtgcgggccc ccgtcaattc atttgagttt
      661 taaccttgcg gccgtactcc ccaggcggtc gatttatcac gttagctacg ggcaccagag
      721 ttaaacccca atccccaaat cgacagcgtt tacggcgtgg actaccaggg tatctaatcc
      781 tgtttgctcc ccacgctttc gcacatgagc gtcagtacat tcccaagggg ctgccttcgc
      841 cttcggtatt cctccacatc tctacgcatt tcaccgctac acgtggaatt ctacccctcc
      901 ctaaagtact ctagttgtcc agtctgaaat gcaattccca ggttaagccc ggggctttca
      961 cacctcactt aaacaaccgc ctgcgtgccc tttacgccca gttattccga ttaacgctcg
     1021 caccccccgt attaccgcgg ctgctggcac ggagttagcc ggtgcttctt ctgtgattaa
     1081 cgtcaatcga ctgttctatt aaaacaatcg ccttcctcgt caccgaaaga actttacaac
     1141 ccgaaggcct tcttcattca cgcggcatgg ctgcatcagg gttcccccca ttgtgcaata
     1201 ttccccactg ctgcctcccg taggagtctg gaccgtgtct cagttccagt gtggctggtc
     1261 atcctctcag accagctaga gatcgcgggc ttggtgagcc tttacctcac caactaccta
     1321 atcccacttg ggctcatctt atggcaggtg gccaaatggt cccacccttt agtcctaaga
     1381 cattacgcgg tattagctac cgtttccagt agttatcccc ctccataagc cagattccca
     1441 agcattactc acccgtccgc cactcgtcac ccaaggagca aactcctatg tgctaccgtt
     1501 cgacttgcat gtgttaagcc tgccgccagc gttcaatctg agccatgatc aaactcttca
     1561 attcaaaaag tttaatcgct caatactgct gacataaaat cgcacttcca aatttaaacc
     1621 taattaaaat taagttaaaa gaaagtaatg aatttctagt tagcacctat taagacttca
     1681 aaattaaaaa aatgtttttc aatcaagtca atcaacaagt gcccacacag attgtctgat
     1741 aaattgttaa agaacaaaaa gaaacgacgc actggaaatc aattttaaat cgttcacaac
     1801 agtgcgtcgt tgtgtgctgc gtattatagg gaaaaacaaa aacaacgcaa gcggtttttg
     1861 taaaaaattt gaaaaaaatg accgcttgcg tatttttacg gcaaaacgag tattaattta
     1921 gctaaacttg cctcttattt gttcttttag cccactttgt ctttcggttt tggcaccaac
     1981 actgtatttc caaacttttt cgctaccaaa taaggtaaat ttatcttttg cccttgtgat
     2041 tgcggtgtaa atcaactctt tcgtcagcac cggggaaatc gaatggggca acaccaatac
     2101 cgtatgttca aattcagaac cttgcgattt atgcacagtc ataatataag ctgcttcata
     2161 atttgaaacc ctacctagag gcaaactatg gtgttctcta ttttcctgta aggaatcaaa
     2221 ataaactcgg ctttctccat tttcgtccgg caaaatgatg ccgacatcac cgctggctat
     2281 gctatttatc ggcatatttt ctgtcattaa aatcggtttt ccgatatagc ggtctcggct
     2341 atgattaaat ttcactaagc tagctttccg caagccttcc gcaatcgcca tattaagacg
     2401 ttcaaccccc agttcactta cccttaaggc tgataaaatt cggacttttt gaaatttctc
     2461 aaaaatcgct ttcacactca ctgaatctgg atttttctcc cgtttttgaa ccgcttgtaa
     2521 ataatcacga taaagttcaa ccgctttatc caccaccatt tgcacacttt tttcaatcca
     2581 gcttgattta tccgttgtct ctttcgtttc cggataacag aaattttcta acttatcact
     2641 gtttttatat ttgtcgaaaa tctcccacga aaggttaatt ttttgatcat ttactgcatt
     2701 tgcaagctct ccaatccagg ctttttcgcc aaatcgatag ctatgtttta agtgagataa
     2761 acaatcacca atcggcaacg cattaccggc ttcaatttgg tagtcggtaa cggcttttaa
     2821 gtaatcgcaa tgggattggc tataaccatt atcaataaaa gagccaagtt cccccataat
     2881 tgaaccagct tccacagaag caagctgatc tttatctccc agcattatca agcgagagtt
     2941 tggtttgagc gcttgaacaa ctttctccat cattgataaa tcaatcattg aagcctcatc
     3001 taatacgacc aaatcatagt gccacggatt tttggtatga tatttggttt tctcagttaa
     3061 tggtctcgca ccaagcaagc gatgaatagt cactgcttca gttggaatag agcgttttaa
     3121 ctcctcagat aacccctttt cttccgccaa cttgctttcc agcgactctt tcattcttgc
     3181 tgctgctttt ccggttggtg cagccagagc gatttgcaaa tatggtttct gtagtgcttg
     3241 ctgttttaat tgcagcccga gtaataattt tactaccgta gttgtttttc cggttcccgg
     3301 cccacctgaa attaagctaa actgcttttc taatgctgtt gcaacggcaa tcttttgcca
     3361 gtcgatagtt tgagctttcc cagtgaatag ttggttgaga atgctttggt ttaattcggt
     3421 atttgcaaat ttttccgaaa aatcaaccgc ttgttgtaaa taaccggcaa tgcgatgctc
     3481 cgcttgccag tagcgataaa aatagagcaa gctgttttgg aataacatcg gtttaattgt
     3541 ttcgggagaa tagctaaaag cgatatgatt tttcagcaca ctctgccatt caagcggtgg
     3601 aatttgctca attttttgca aaatttgttc gagcaaatga taagtttgtg gcttgctatt
     3661 taattcaaaa agagaagaaa gactgagatt ttccaaacgt aatgccgtat gcccaagtcc
     3721 cacttggtaa gaaaccaacg cagcggctaa cacagctaaa tcttgctcca aaggcgaata
     3781 cccatagggt ttctgcttat tatcaataaa acgggcaaat tgataattta agtcagaaat
     3841 cgccttttct tgtttaagtt ccaataataa attcagcata atcaaacctt aaatcgacca
     3901 cctaacatta agtgat
//
LOCUS       DANLXS010000208         3891 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.211, whole genome shotgun sequence.
ACCESSION   DANLXS010000208 DANLXS010000000
VERSION     DANLXS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.211"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            80..2575
                     /locus_tag="PXD71_002377"
     CDS             80..2575
                     /locus_tag="PXD71_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HDL6292442.1"
                     /translation="MSQAVFYLNNFKELFMTVEVGGVVITADLEFENLLRDTQKVDKA
                     MDDIGKSTLKAEKGLKGLETQATKTAIAVNQATNKMKNIRGVAGQLGYQIQDISIMLQ
                     MGSNASTVIAQQGSQILSIFGPMGAVAGAILAISGAIGGALLPSLFDSTDATELLAAA
                     QKNLSEVVTQTESGVSALSAKIIKLAKVSEDAAKAKIAVAMSDAKIAIKAAGDSSQKA
                     FEQFDGFFGSFTNSSQSIRNAIDELKRYESKGKDVGAVIKELGGTYGGAIAEVNALDS
                     VTSEMSKTLGITQSEALGLIKLLAQLEQDKSPDNIKLLAIALSDLSDKNGWANEELNK
                     LTRTINDNSVSALDAETSVRLLESAIKDLQNAIQNSQGALDGNVAKMNQLADAAERKA
                     ATIGKTERETAKYAATLLATTDEAKKNLDSTLSRIDASYDQIEAYQAEQKALQDKKTA
                     DAKAAREAEAAAKKSAQAKKQVVEQLRQMATQYEIAVLRQKGMHLEAVKLEAGMRLGA
                     AATKEQREEAEKLAQSIYGATAASQNFNAIQAQASPVTALDQRHQQQLAQIEEYKILY
                     PQKIMEAEAARAAIEEQYRQQRMEAQWEEWKQASDGARMFGDAVDAVGSSATSTITGL
                     LNGTMSVRDAFASIANTILNSVVQSLVEMGMAQVKQMIMGQTAAKASMAAQMVQAKAL
                     AASFAPAASMVSLATQGANAIPAQAAITSTMSMAQVAAVTGRKLGGPVSARQMYRVGE
                     NNQPEIFKASNGMQYMIPGQSGRVFSNKQSQGQGGGESKTVAVIVNQTNHFSSDEASA
                     NQAAVAKALSNQIKASVRLELQSQMRAGGVLAR"
     gene            2579..2908
                     /locus_tag="PXD71_002378"
     CDS             2579..2908
                     /locus_tag="PXD71_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292443.1"
                     /translation="MQTFNFKVEQDYTVQNEPSVVILKFADGYEQTAPKGLNHNLKKY
                     QGITVKANKAEAVKIQQFLNAHGGYKKFNWRDRTSDETVTVRCRSWSSTKLGAVIEFT
                     LNFDEVI"
     gene            2908..3378
                     /locus_tag="PXD71_002379"
     CDS             2908..3378
                     /locus_tag="PXD71_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL6292444.1"
                     /translation="MPKAPPKKMASELIKLEQDALIELWEIDLTHIYSSSNPNQRGEI
                     FRFHNGVSQTQQNIWWQGNEYQAYPIAADGFEISGQGPSNRPTLTVSNLYGVVTVLVE
                     DFGQPVEAKVSSEETVDGNIQMTVELLKKMDQIADQRYRRNQLNDLRTGGTLSK"
     gene            3386..>3891
                     /locus_tag="PXD71_002380"
     CDS             3386..>3891
                     /locus_tag="PXD71_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL6292445.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRKLQDKNGNAGSVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEATGFERNSSGPSNRPTLTLSNLFGLVTGIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLSSLTRETATFTLA
                     LPIETDNA"
BASE COUNT         1301 a          775 c          899 g          916 t
ORIGIN      
        1 aaaatataga ttaaattgta tttaaccaat ctattgctag ataaattgta aaaaaatcac
       61 agaaataacc gcttgtgcta tgtcacaagc ggttttttat ttaaataatt ttaaggaatt
      121 gtttatgacg gtcgaagttg gcggagttgt aattaccgca gatctagaat ttgaaaatct
      181 gctaagagat actcaaaaag ttgataaagc gatggatgac atcggaaaat caacattaaa
      241 agcagaaaaa gggctgaaag ggctagaaac gcaggcaacc aaaacggcga tagcagtaaa
      301 tcaagccaca aacaaaatga aaaatatccg tggagtagca ggtcaacttg gttatcaaat
      361 tcaagacatc tcaataatgc ttcaaatggg ttctaacgct tcaaccgtta ttgctcagca
      421 agggtcgcaa atcttaagta ttttcggtcc aatgggagct gttgcagggg cgatcttagc
      481 tattagtggt gctattggtg gggcgttact ccctagtttg tttgattcaa ctgatgcaac
      541 tgagcttttg gcagcagctc aaaaaaatct cagcgaagta gttactcaaa cggaaagtgg
      601 tgtttccgcc ctttctgcga aaatcattaa attagcaaaa gtaagcgaag atgcagcaaa
      661 agcaaaaatt gcagtagcta tgagtgatgc aaaaatagct attaaggcag caggggattc
      721 atcacaaaag gcatttgagc aatttgacgg cttttttgga tcgtttacaa attcaagtca
      781 aagtattcgg aacgcaattg acgaattaaa acgctatgaa tcaaaaggta aagatgttgg
      841 tgccgtaata aaagaacttg gaggcacata tggtggtgca attgccgaag ttaatgcgct
      901 ggattctgtg acaagcgaaa tgtctaaaac gcttggtatt actcaatctg aggctcttgg
      961 tttgataaaa ctgttggctc aattagagca agacaaaagc cctgataata ttaaattact
     1021 cgcgatagct ttatctgact taagcgataa aaatgggtgg gcgaacgaag agctaaataa
     1081 gctaaccaga acgataaacg ataattcagt atctgcttta gatgccgaaa catcagtaag
     1141 actcttagag tctgctataa aagacttgca aaatgcaatc caaaactccc aaggtgcatt
     1201 ggatggcaat gtagcaaaaa tgaatcaact tgcagatgcg gcagaaagaa aagctgcaac
     1261 gataggcaag actgagcgtg aaactgctaa atatgcagct acgctattag ctacaaccga
     1321 tgaagctaaa aaaaatcttg attccacatt aagtaggatt gacgcatcat acgatcaaat
     1381 cgaagcctat caagccgaac aaaaagcctt acaagataaa aagaccgcag atgcaaaagc
     1441 tgccagagaa gctgaagcgg ctgcaaaaaa atccgctcaa gccaagaagc aagttgtcga
     1501 acagttgcga caaatggcta cacagtacga gattgctgtt ttgaggcaaa agggaatgca
     1561 tctcgaagcc gtaaaattag aagctggtat gcgtttagga gctgcggcaa caaaagagca
     1621 acgagaagaa gctgaaaaac tagcacaaag tatttatgga gcaaccgcag caagtcagaa
     1681 ctttaacgct atacaagccc aagcatctcc tgttactgca ttagaccaac gacatcaaca
     1741 acagctagct cagatcgaag aatataaaat cttgtatcca caaaaaataa tggaagcaga
     1801 agctgcgaga gctgcaatcg aagaacaata ccgccagcaa cgaatggaag ctcaatggga
     1861 ggagtggaag caagcgagcg acggggcgag aatgtttggt gatgcggttg atgctgttgg
     1921 ttcaagtgca acaagcacaa ttacggggct actaaatggc acaatgtccg tgcgtgatgc
     1981 tttcgcatca attgcaaata cgatcttaaa tagtgtagta caaagccttg tcgaaatggg
     2041 tatggctcaa gttaagcaaa tgattatggg gcaaactgct gccaaggctt ctatggcggc
     2101 gcaaatggtg caagctaaag cgttagctgc ttcatttgca cccgcggcct ctatggtttc
     2161 tcttgccaca cagggggcga atgctattcc cgctcaagct gcgatcacgt caactatgag
     2221 tatggctcaa gtcgccgcgg tgacaggtcg taaacttggc ggccctgtat cagctagaca
     2281 gatgtatcgt gtcggcgaga acaatcagcc cgaaatcttc aaagcgagca atgggatgca
     2341 atatatgatc cccggtcaaa gcggtcgtgt attcagcaac aagcagtcgc aaggtcaagg
     2401 tggaggcgaa agcaaaacag tagctgtcat cgtaaatcaa accaatcact ttagcagcga
     2461 tgaagccagt gcaaatcaag cggctgttgc aaaagcattg agcaatcaaa tcaaggcttc
     2521 cgtgagattg gaattgcagt cacaaatgcg agctggtgga gttctagcga ggtaatcaat
     2581 gcaaacattt aatttcaagg ttgagcaaga ctacactgtt caaaatgagc ccagtgtcgt
     2641 catactaaaa tttgccgatg gttacgagca aaccgctccc aaagggttaa accacaatct
     2701 gaaaaaatac caaggcatta ccgtaaaagc gaacaaagca gaagcggtga agattcagca
     2761 atttctcaat gctcacggtg ggtataagaa attcaactgg cgcgatagaa caagcgatga
     2821 gacggtaacg gttcgttgca gaagctggag ctccactaaa ttaggtgcag tcattgagtt
     2881 tacacttaat ttcgatgagg tgatttaatg ccaaaagccc cccccaaaaa aatggcatca
     2941 gagttgataa aactcgaaca agatgccctg attgagctgt gggagataga tctcactcac
     3001 atctactcta gctcaaaccc aaatcagcgc ggagagatat tccgctttca caatggcgtg
     3061 agccaaacac aacagaacat ctggtggcaa ggcaatgaat accaagcata cccgattgcc
     3121 gccgatggtt ttgagatttc gggtcaagga ccgtcaaatc gtccaacact cactgtctct
     3181 aacctgtacg gtgttgtcac ggtattagta gaagatttcg ggcaacccgt agaggcgaaa
     3241 gtcagcagtg aagagactgt agatggcaat atccaaatga cggtggaact gctgaaaaaa
     3301 atggatcaaa tcgccgatca acgctaccgt agaaatcagt taaatgattt aagaacaggt
     3361 ggaacattat ctaaataggt gaagtatgcc aattagtatt tctaaccaaa tgaaactaga
     3421 tcttgccaaa cttgagcaaa atgcaatgct ggacttgtac gaggttgatt tgcgtaaatt
     3481 gcaagacaaa aacggcaatg ctggtagtgt ttaccgcttt tattctgggc taaatgaact
     3541 caaaacaagt attgtctggc aaggtcgaac ttacgaccct taccccatcg aggcaacagg
     3601 atttgagcgt aacagcagcg gcccaagcaa tcgccctact cttacgctat caaatctgtt
     3661 tggcttagtt acgggtattg ctaatcagtt tgacgaatgt atcggtgcga ttgttcgccg
     3721 tcatcaggtt tacgcccaat atttagatgc agtaaacttt gctggaggta atgccaaagc
     3781 cgatcctaat caggaaatca tcagccattt tgtgattgag caactttcaa gcctcacacg
     3841 agagactgct acttttacgc ttgcgttgcc gattgaaacc gataatgcca a
//
LOCUS       DANLXS010000209         3848 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.212, whole genome shotgun sequence.
ACCESSION   DANLXS010000209 DANLXS010000000
VERSION     DANLXS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.212"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            32..2071
                     /gene="malQ"
                     /locus_tag="PXD71_002381"
     CDS             32..2071
                     /gene="malQ"
                     /locus_tag="PXD71_002381"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL6292446.1"
                     /translation="MPYFFDERGVKKTASEKIKHSLFKAFNGNTQARETPIPTVNIFY
                     QHQPCFLPFHLADKKHPLTGTWQLQLENSNQIISGKIKARGITLPQDLPLGYHQLQLK
                     TVDKTFNCTIIIAPERCYQPQAVIEQKRLWGTFLQLYTLKSAHNWGIGDFGDLKDFIQ
                     QIAPYQADFIGLNPIHSLFPANPNSASPYSPSSRRWLNIAYIDINQLAEFQQSDEAQT
                     WFHSAEIQQQLSELRTQDWLNYEQIIPLKLKGLRFAFAKFQQNMTACAAPAFIEFVQK
                     GGESLQVQATFDALHQHLSAQFADQWGWDFWEKAYQDYHSAAVQQFRETHKTDIEFYL
                     WLQFVADQQLAECDAICRQQNMRIGMYRDLAVGVSPNGAETWNDKQLYCLNASVGAPP
                     DVLGPQGQNWGLTPMNPHVLKQQAYRPFIELVRANMSHCGALRLDHIMSLLRLWWIPK
                     GDSAVNGAYIRYPVDDLIAILALESQRHQCLIIGEDLGTVPKEIVSKLKNAGILSYKI
                     FYFEFDEQGKSRDLQHYPYQAMTTLSTHDLPTINGYWRGYDFELGEKFGVYPNSNILA
                     ILKQDRVNAKAKILQRLQEHNVEIAPEISEQLNSPVNTAFIHCLQTYVANVSSGLFGF
                     QPEDWLGMTEPVNIPGTSTQYANWRRRLSVNLEEIFTDKDIQALLKEVNLIRKNQ"
     gene            complement(2136..3797)
                     /locus_tag="PXD71_002382"
     CDS             complement(2136..3797)
                     /locus_tag="PXD71_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDL6292447.1"
                     /translation="MSEIALTVSLLSLVAVLGLWIGHIKIRGVGLGIGGVLFGGLFVS
                     HFMAQFDVKLDAHTLHFIQEFGLILFVYSIGIQVGPGFFASLKHSGLKLNGFAVLIVL
                     ISGILVILIHKFFNVPLPVILGIFSGAVTNTPSLGAGQQVLAELSAESVTEIMGMSYA
                     IAYPFGIIGILLSMWLIRILFRINIEAEAEAFNKQQNRSKDQLSTLNVRLTNPNIHGL
                     TLKQIPDFNLHEVAYSRLKRGNELSIPRVDTILQIGDILHLVGEESSLRKMQIILGEA
                     VDVSVSTKGTIYRSERAVVTNEKVFGKQIRQLMLKGKYDVVISRLNRAGVELVPTGDM
                     TLQFGDVLNLVGRQADIEMVMTIIGNAHSKLQQVQMLPIFIGIGLGVLLGSVPIYLPG
                     FPVALKLGLAGGPLVVALILARIGSFSKLYWFMPPSANLALREIGIVLFLAVVGLKSG
                     GRFLETLLSSEGVEWICYGAIITFVPLMIAGLIARMYNKVNYLTICGLLAGSMTDPPA
                     LAFANEIKEGNGAAALSYATVYPLVMFLRIMLPQVLAIMLWVSGG"
BASE COUNT         1237 a          876 c          696 g         1039 t
ORIGIN      
        1 aactgccaaa caatattatc gggctggaat tatgccctat tttttcgatg agcgtggtgt
       61 aaaaaaaaca gcatctgaaa aaataaaaca ttcgttattc aaagccttta acggcaatac
      121 tcaagcaaga gaaacaccta tccctacggt caacattttt taccagcacc aaccctgttt
      181 tttacctttt catttagcgg ataaaaaaca tccgttaacc ggtacttggc agctacaact
      241 agaaaattca aaccaaatta tttccggcaa aattaaagca aggggaatta cattacctca
      301 agatttgccc ttaggttatc accaattaca gctaaaaacg gtagataaaa cctttaattg
      361 caccattatt atcgctccgg aacgctgcta ccaaccacaa gccgtcattg aacaaaaaag
      421 attgtgggga accttcctac aactatacac cttaaaatca gcccacaact gggggattgg
      481 cgattttggc gatttaaaag acttcattca acagattgcc ccttaccaag cagattttat
      541 cggcttgaac ccgattcact cactttttcc ggcaaacccg aattctgcca gcccatacag
      601 tccatcctct cgccgatggc tgaatattgc ttacattgat atcaatcagc tagcggaatt
      661 tcaacaaagt gatgaagcac aaacttggtt tcattctgct gaaatccaac aacaattatc
      721 agaattacgc actcaggatt ggttgaatta cgagcaaatc attccgttga aattgaaagg
      781 gctacgcttt gctttcgcaa aatttcagca aaatatgacc gcttgcgctg cccctgcttt
      841 tatcgaattt gtacaaaaag gtggtgaaag cttacaagtt caagccacat ttgatgccct
      901 tcatcagcat ctatcggcac aatttgccga ccaatggggc tgggatttct gggagaaagc
      961 ctaccaagac taccactccg cagccgtgca gcaatttaga gaaacgcata aaaccgacat
     1021 agaattttac ctttggttac aattcgttgc cgatcaacag ctagcagaat gtgatgctat
     1081 ctgccgtcag caaaatatgc gtattggtat gtatcgcgat ctcgccgttg gcgtcagccc
     1141 gaatggtgca gaaacgtgga atgacaaaca actttattgc ttaaatgcct ctgtcggcgc
     1201 tccgcctgat gtattaggtc cgcaaggaca aaactggggc ttaaccccaa tgaatccgca
     1261 tgtattaaag caacaagcct accgtccgtt tattgagtta gtcagagcta atatgagtca
     1321 ttgcggtgca ttacgccttg accatattat gtctttactc cgtttatggt ggatccccaa
     1381 aggcgatagt gcggttaatg gtgcttatat tcgttatcca gttgatgatt taatcgcaat
     1441 tttggcactt gaaagccaac gccaccaatg cctaattatc ggtgaagatt taggtactgt
     1501 gccaaaagaa attgtgagca agctcaaaaa tgcggggatt ttatcttaca aaattttcta
     1561 ttttgaattt gatgaacaag ggaagagtcg agacttacag cattaccctt accaagcaat
     1621 gaccacatta agcacccacg atttaccgac tatcaatggt tattggcgtg gctatgattt
     1681 tgaattaggc gaaaaattcg gcgtctatcc taattcgaat attttggcaa tccttaagca
     1741 agatcgagtc aatgccaaag ccaaaatctt acaacgctta caagaacata acgtagagat
     1801 cgcacctgaa attagcgagc aacttaattc tccggtgaat accgcattta ttcactgttt
     1861 acaaacctat gttgcaaatg taagcagcgg attattcggc ttccaacccg aagactggtt
     1921 gggtatgact gagccggtta acattccggg aaccagtaca caatatgcaa actggcgacg
     1981 tcggcttagt gttaacctcg aggagatctt cacagataaa gatattcaag ccttattaaa
     2041 agaagttaat ttaatccgta aaaatcaata aaagttagat aagaaaaaag gcgactttcc
     2101 gtcgcctttt gggcttatat gcttgatgtc tatctttatc cacctgaaac ccacaacata
     2161 atcgctagca cttgaggtag cataatacgc aaaaacatta ctagtgggta aacagttgca
     2221 taggataacg ctgcagcgcc atttccctct ttaatttcat tggcaaaagc aagcgctgga
     2281 ggatcagtca ttgagccggc aagtaatcca caaatcgtta aatagtttac tttgttatac
     2341 attctggcaa tcaatcctgc tatcatcaat ggaacaaaag taataatcgc cccatagcat
     2401 atccactcaa cgccctcact gcttaatagc gtttcgagaa atctaccgcc agattttaaa
     2461 ccaactaccg ctaaaaacag tacaataccg atttcacgca atgcaagatt ggcacttggt
     2521 ggcataaacc agtagagttt actgaaacta ccaattcttg caagaataag agcaaccacc
     2581 agtggtccac cagccaagcc cagctttaac gcaacaggga aacccggtaa ataaatcggt
     2641 acagaaccaa gtaatactcc caaaccgatc ccgataaata ttggcaacat ttggacttgt
     2701 tgcaactttg agtgcgcatt gccgataatc gtcattacca tttcaatatc agcttgccgc
     2761 cctactaaat tgagaacatc accaaattgt aacgtcatat cacctgttgg tactaattct
     2821 acaccggctc gatttaaacg agaaatgaca acatcgtatt tgcctttaag cattagctga
     2881 cgaatttgtt tcccgaagac tttttcattc gtcactacag cacgttcaga acggtaaatc
     2941 gtgcctttgg ttgagacaga tacatctact gcctcaccta aaataatctg catttttcgt
     3001 aatgaggatt cttcacctac taagtgtaaa atatctccta tctgtagaat cgtatctaca
     3061 cgtggaatag aaagctcatt cccccgtttt aagcgtgaat aagctacttc gtgcaaatta
     3121 aaatcaggaa tttgtttcaa cgttaaaccg tgaatatttg gattagtcag acggacattt
     3181 aaggtactaa gttgatcttt acttctattt tgctgtttgt taaaagcctc tgcttccgcc
     3241 tcaatattga ttcgaaataa aatccgaatc agccacattg aaagtagaat tccgataatg
     3301 ccgaacgggt aagcaatcgc ataactcatt cccataattt cagttacact ctcagcgctt
     3361 aactcagcca atacctgttg acctgctcct aaagaagggg tattggttac agccccggag
     3421 aaaatgccta aaatcacagg caaaggaaca ttgaagaatt tatgaataag aataactaaa
     3481 ataccgctaa tcagtacaat cagtaccgca aaaccattga gttttaagcc agaatgtttt
     3541 agtgaggcaa agaatccagg tcctacttga attccaattg aataaacaaa taaaatcagc
     3601 ccgaactctt gaataaaatg aagcgtatgg gcatcgagct tcacatcgaa ttgcgccata
     3661 aaatgggaga caaacagccc accaaataac actcccccta tacctagccc cacgcctctg
     3721 attttaatat ggccaatcca taagcctaga acggccacta aagataataa actgacagta
     3781 agtgcaattt cgctcattac agcctctctt taatatgaaa tacctcaaaa atggtatcca
     3841 attctacc
//
LOCUS       DANLXS010000210         3783 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.213, whole genome shotgun sequence.
ACCESSION   DANLXS010000210 DANLXS010000000
VERSION     DANLXS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.213"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(162..1664)
                     /gene="ppx"
                     /locus_tag="PXD71_002383"
     CDS             complement(162..1664)
                     /gene="ppx"
                     /locus_tag="PXD71_002383"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDL6292448.1"
                     /translation="MSSNIAPREFAAIDLGSNSFHMIVARIVNGSIQVLSRLKRRVRL
                     ADGLDENLVLSQDAIDRGIACLALFAKRLEGFSAENVKVVGTYTLRRAINNQAFLEQA
                     KAVFPYPISIISGQQEARLIYSGVSHTQPETGRKLVVDIGGGSTEMTIGDDFTPIRAE
                     SRHMGCVSFAKRFFPQGELTIERFDSAYQLAMEKIEDLMWEYKELGWDYALGSSGTIK
                     TVHQVLIANGYRDGLITESRLQKIIDICLEFKSLNEIKLRGLLDERADVLVPGLAILL
                     ALFHTFRIDVLRYSNGALREGVMYGLEKSFQVGDIRQRTAEALAEQFALDKQQAKRVE
                     HTALILFDQIKTWKNRRQVQDLRSMLKWASLLHEIGIVVNHNNVHRHSAYLIANRDLP
                     GFDFEQQTLLSVLMRYHLKGFKRSDIRSINRYQCRDVLTLVRVFRLAVLLNRSRQATV
                     VPKQLNLVIENNAWQLTFEQGFLANNPLISIDLEEEQKILASTNLQFAVS"
     gene            1860..2189
                     /locus_tag="PXD71_002384"
     CDS             1860..2189
                     /locus_tag="PXD71_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDL6292449.1"
                     /translation="MSEVNKSFHDMLEYVHLYRLKNKLHRETADNDRKIRDNQKRVLL
                     LDNLTQYITDEMSIEEVRAIISNMRDDYENRVDDYMIRNAELSKQRREIRQKMASHKQ
                     APTNKKA"
     gene            complement(2253..3683)
                     /locus_tag="PXD71_002385"
     CDS             complement(2253..3683)
                     /locus_tag="PXD71_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDL6292450.1"
                     /translation="MSTLTYLQKIGRALMVPVAALPAAALLMGIGYWIDPDGWGTNSQ
                     LAALLIKSGAAIIDNMSILFAVGVAFGMSKEKDGAAALSGLVGYLVITTLLSPGTVAQ
                     LKGIAVDQVPAAFGKINNQFIGILVGVISAEIYNRFYQVELPKALSFFSGKRLVPIVV
                     SFILLYVWPVIFGALVSFGESIKDMGALGAGIYAFFNRLLIPVGLHHALNSVFWFDVA
                     GINDIPNFLGGAKSLAEGKAVIGVTGMYQAGFFPMMMFGLPAAALAIYHSAKPAKKMQ
                     VASIMFAAALASFFTGITEPLEFAFMFVAPVLYVVHALLTGLSVYIAASMEWIAGFGF
                     SAGLVDLVLSTRNPLAVQWYMLLAQGIVFAVIYYVLFRVVIQAFNLKTPGREEDSEVQ
                     TVNTVSRSEQAAKFVEALGGKENITSIDACITRLRLTLNDTAKADETKLKALGSKGNI
                     KLGENGYQVILGPEAEFVADAMKKLN"
BASE COUNT         1238 a          858 c          653 g         1034 t
ORIGIN      
        1 attcgtaaaa tatataatct caataagcaa acatcaaaaa tgagaagttg atcaaatttt
       61 aaaaaatact ttttgcaaaa aattagctaa atttgaccgc ttgttcgcaa aagggaatgc
      121 aataaacgaa aaaggaagcc ttcgcttcct tcatttaaat gttaagagac cgcaaactga
      181 agatttgtac tcgccaatat tttttgctct tcttctaaat ctattgaaat cagtggatta
      241 ttcgctaaga agccctgttc aaaggtaagt tgccaagcat tattttcaat caccaaattc
      301 aactgtttcg gcactacagt cgcttggcga gaacggttta atagcaccgc tagccggaaa
      361 acccgtacca acgttaatac atctctacat tggtaacggt tgatactacg aatatccgag
      421 cgtttgaatc ctttaaggtg gtatcgcatt aataccgata gaagggtttg ctgttcaaaa
      481 tcaaatcccg gtaaatctcg gtttgcaatc aaataagcag aatgacgatg cacattattg
      541 tggtttacaa caataccaat ctcgtgtaat aaacttgccc atttcagcat tgaacgcaga
      601 tcttgcactt ggcggcggtt cttccaagtt ttgatttggt caaataaaat caacgccgta
      661 tgctccaccc tttttgcttg ctgtttatct aaagcaaatt gctcagctaa cgcttctgca
      721 gtccgttggc gaatatcccc aacttggaaa cttttctcca aaccatacat taccccttct
      781 cttaaagccc cgttggaata acgtaataca tcaattctaa aagtgtgaaa aagtgctaaa
      841 agaatagcta agcccggaac tagcacatct gctcgttcat caagtaagcc tcgtagctta
      901 atctcattta acgatttaaa ctctaggcaa atatcaataa ttttctgtag gcggctttct
      961 gtaattaagc catcacgata gccattggca atcagcactt gatggacggt tttaatcgta
     1021 ccggaagaac ctaaagcata gtcccaacct aactctttgt actcccacat taaatcttcg
     1081 attttttcca ttgcaagctg ataggcacta tcaaaacgct caatcgttaa ttccccctgc
     1141 gggaaaaagc gttttgcaaa actcacacaa cccatatgac gactttccgc tcgaatcggg
     1201 gtaaagtcat caccaatagt catttccgtt gaaccaccgc caatatctac cactaatttt
     1261 ctgccagttt cgggttgggt atggctaacg cccgaataaa tcagcctcgc ctcttgttgt
     1321 ccggaaataa tggaaatcgg gtaaggaaat actgctttcg cttgctccag aaaagcttgg
     1381 ttattgattg ctctacgtaa ggtataagtg ccaaccacct tcacattctc tgcagagaac
     1441 ccttctaaac gttttgcaaa taaagctaaa caagcaatgc ctctatcaat cgcatcttgg
     1501 cttaatacta agttttcatc aagcccatct gccagccgta cacgccgttt caaacgggaa
     1561 agcacttgaa tggaaccatt tacgatacgg gctacaatca tatgaaagct gttcgagcct
     1621 aaatcaatag ctgcaaactc tctaggagca atatttgatg acataatgaa accctgtacc
     1681 aataactgaa ataagtaaga tattaccatt tttgagccct agatatcact acaaatttag
     1741 cgttattcgc gagaattaat ttaaatcata aaatgtgata aatttttttg acgaattgac
     1801 ggaaaaagaa taggatttac gagcttactt aattcaacac tacatcaagg gagattgata
     1861 tgtctgaagt gaataaatca ttccacgata tgctggaata tgtgcatctt tatcgtctaa
     1921 aaaacaaact gcaccgtgaa acggcggata atgatcgcaa aattcgagat aatcagaagc
     1981 gtgtactttt attagataac ctaacgcaat atattaccga cgaaatgtct atcgaggaag
     2041 ttcgagcgat tatttccaat atgcgtgatg actatgaaaa tcgagtagat gattatatga
     2101 tccgtaatgc ggaactttcc aaacaacgcc gtgaaatccg ccaaaaaatg gcctctcaca
     2161 aacaagcacc aaccaataaa aaagcataaa aagccaagcg gtctgatttg caaaaaaata
     2221 tgcaaatcag accgcttgtg attaagcaga gattaattca gctttttcat tgcatctgcc
     2281 acaaattctg cttccggtcc caaaatcact tggtagccat tctcgcctaa tttgatatta
     2341 ccttttgacc ctaacgcttt caatttggtt tcgtctgctt tcgccgtatc atttaaggtt
     2401 aggcgtaagc gggtaataca agcatcaatg ctggtaatat tctctttacc acctaatgct
     2461 tcaacaaatt tggcagcttg ttcgctacga gaaacagtat tcactgtttg tacttcgcta
     2521 tcttcctcac gccccggcgt ttttaaatta aaggcttgga tcactactcg gaacaataca
     2581 tagtaaatta ctgcgaatac gataccttgg gccaataaca tataccactg cactgccagc
     2641 ggatttcgtg tcgataacac taaatcgacc aaccctgcac taaaaccgaa accggcaatc
     2701 cattccattg aggctgcaat ataaacagat aatccggtta ataatgcgtg aaccacataa
     2761 agcacaggtg caacaaacat aaaggcaaat tcaagcggtt ccgtaatgcc ggtgaagaac
     2821 gaagctaaag cagcagcaaa cataatagag gctacttgca ttttcttagc cggtttcgca
     2881 ctgtggtaaa tagctaatgc tgccgcaggt aagccaaaca tcatcatcgg gaagaaacca
     2941 gcttgataca tacctgttac tcctatcact gctttgccct cagccaatga ttttgcccca
     3001 cctaagaagt ttggaatatc attaatgcct gcaacatcaa accagaaaac cgagtttaat
     3061 gcgtggtgta atccaaccgg aattaataaa cgattaaaga aagcataaat acctgcccct
     3121 aacgccccca tatctttgat agattcccca aaagagacta aagcaccaaa aataaccggc
     3181 cagacataaa gcaagataaa tgacaccaca atcggcacca atcgtttacc gctaaagaaa
     3241 gacaatgctt tcggtaactc tacttggtag aaacggttat aaatttcggc tgaaataacc
     3301 cctactaaga tcccgataaa ttggttgttg attttaccga atgctgcagg cacttgatca
     3361 actgcgatcc cttttagctg agcaacggtc cccggagaca acaaagtggt aattactaaa
     3421 tacccgacta aaccggataa tgctgctgcc ccatcttttt cttttgacat accaaatgcc
     3481 acacccacgg caaatagaat agacatatta tcaataattg ccgccccaga tttaatcagc
     3541 aatgccgcta attggctatt tgttccccag ccgtccggat caatccaata gcctataccc
     3601 atcaataatg ctgctgctgg taatgcagca accggcacca ttaatgcacg cccaattttt
     3661 tgtaaatagg ttaatgtact cataaaagcc tccaaaaata cctcataatg agtaacttaa
     3721 gcatagaaaa aaaccgatta aagaacaatc aaataaattt ggttttgtga tccatttcaa
     3781 aat
//
LOCUS       DANLXS010000211         3767 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.214, whole genome shotgun sequence.
ACCESSION   DANLXS010000211 DANLXS010000000
VERSION     DANLXS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.214"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            189..521
                     /locus_tag="PXD71_002386"
     CDS             189..521
                     /locus_tag="PXD71_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF413 domain-containing protein"
                     /protein_id="HDL6292451.1"
                     /translation="MADSFSVTRRFFDDKNYPRGFSRHGDYTIRESQTLEQFGQACLA
                     LESGERKPATQEEKRFVAVMKGEEVAETDIEKAWLKYRTLTSKTKRIYTLSGNAGSDA
                     GDDFSAAE"
     gene            586..1677
                     /gene="trmA"
                     /locus_tag="PXD71_002387"
     CDS             586..1677
                     /gene="trmA"
                     /locus_tag="PXD71_002387"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDL6292452.1"
                     /translation="MTLPIEQYSDLLAKKTENLTALLKPFNPPAIEVFASKPSHFRMR
                     AEFRVWHEEGELYHIMFNPETKARYRVDSFPIASELINHMMTALLAEIKGNELLTRKL
                     FQIDYLSTLSGEIAVSMLYHKSLNEEWVEQANALKSRLEQQGFKVQIIGRANKQKIAL
                     DCDYVEEILPVHGKNYVYRQVENSFTQPNAEMNIKMLEWARNCTKNSEGDLLELYCGN
                     GNFSIALAENFRKVLATEISKSSVQSAQYNIEKNGIVNLQIIRMSAEEFTQAMNGVRA
                     FYRLKGIDLNAYECNTIFVDPPRAGLDQATLNMVQAYERILYISCNPETLAENLQQLS
                     QTHRIEKAALFDQFPYTHHIESGVWLIRK"
     gene            1769..1858
                     /locus_tag="PXD71_002388"
     tRNA            1769..1858
                     /locus_tag="PXD71_002388"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1803..1805,aa:Ser,seq:tga)
     gene            2014..2385
                     /locus_tag="PXD71_002389"
     CDS             2014..2385
                     /locus_tag="PXD71_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDL6292453.1"
                     /translation="MKYLYIICGFICIGLGIIGAVLPGLPTTPFLLLALFCFTKGSER
                     IRQWFIQTKLYKKYLQEYDEKRAMTMKQKITILAISFPFTLFAFFALPHIGGKIALVV
                     LVIIQYWYFFCKMKTLEPENA"
     gene            complement(2382..3293)
                     /gene="xerC"
                     /locus_tag="PXD71_002390"
     CDS             complement(2382..3293)
                     /gene="xerC"
                     /locus_tag="PXD71_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL6292454.1"
                     /translation="MTTSNPLFQQTRPYWDFLRIEKQVSPHTLSNYQRQLMAVSELFS
                     QAGIENWEEVESSSVRWMLSQSHKQGLSAKSIGLRLVALRQWFVYLIKIGRMSANPTL
                     GVKSPKVGKHLPKNIDAEQVGQLLAIESDEPSDIRDIAMMELMYSSGLRLSELQGLDL
                     GDMDLSAREVRVQGKGSKERIVPIGSQALKALNRWLEMRLQFKPQDNAVFLNKRGGRL
                     SHRSIQLAMKKWGERQGLESHLHPHKLRHSFATHMLEASGDLRAVQELLGHSSLSTTQ
                     IYTHLDFQHLAKIYDSAHPRARRKQED"
     gene            complement(3360..3650)
                     /locus_tag="PXD71_002391"
     CDS             complement(3360..3650)
                     /locus_tag="PXD71_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5377 domain-containing protein"
                     /protein_id="HDL6292455.1"
                     /translation="MSVKTEVLFSNHWNVRVSDPGFEGSPSHYFETVYLTLEAFINGE
                     NVHYEFTRKVEQEVKTHRSFTDVNELFEFLGEYLSPVALGNVGVRIGQLGLA"
BASE COUNT         1115 a          825 c          732 g         1095 t
ORIGIN      
        1 aatataaaaa atatacctca gactgcattg tctattttaa aaggatcaat atttctcctt
       61 aaagagtttt ttgcaaaaaa tttgccaaat ttgaccgctt ttttacatta aggcattgat
      121 ttattcgctg tttttgttta atgtatgccg attttatttt ccctaatttc aaacaacgag
      181 gcattattat ggcagacagt ttcagcgtta cgcgccgctt ttttgacgat aaaaattatc
      241 cacgtggttt ctctcgccac ggcgattaca ccattcgtga atcacaaact ttagaacaat
      301 tcggtcaagc ttgcttggct ttagaaagtg gtgaacgtaa gccggcaact caagaagaaa
      361 aacgtttcgt tgcggtaatg aaaggcgaag aggtagcaga aactgacatt gaaaaagcgt
      421 ggttaaaata ccgcacatta actagtaaaa caaaacgcat ctacaccctt tcaggcaatg
      481 caggtagcga tgccggcgat gacttcagtg ctgctgaata atttctaatt taaaaccacg
      541 aagtaattcg tggtttgttt tttcatttgc tatttgtggt aaaaaatgac tcttcctatc
      601 gaacaatatt ccgatcttct tgcaaaaaaa accgaaaatt tgaccgcttt actcaagccg
      661 tttaacccgc ctgcgattga ggtatttgcc tcaaagccaa gccatttccg tatgcgggca
      721 gaatttcgag tgtggcacga ggaaggcgag ctgtaccaca ttatgttcaa ccctgaaacc
      781 aaagctcgtt atcgtgtgga tagcttccct attgccagtg agctgattaa ccatatgatg
      841 accgcattat tggcggaaat taaaggcaat gaactactga ctcgcaagct gttccagatc
      901 gattatttaa gcaccttaag cggtgaaatt gcagtctcaa tgctttacca caaaagcctg
      961 aatgaagaat gggttgaaca ggcaaacgcc ttaaaaagcc gtttagagca acaaggtttc
     1021 aaagtgcaaa ttatcgggcg tgccaataaa caaaaaatcg cactggattg cgattatgtg
     1081 gaagaaattc taccggtaca cggcaaaaat tatgtgtatc gccaagtgga aaacagcttt
     1141 acccagccga atgcggaaat gaatattaaa atgctggaat gggcgagaaa ttgcacaaaa
     1201 aatagtgaag gcgatttgct tgagctttac tgcggcaacg gcaatttttc gattgccttg
     1261 gcggaaaact tccgcaaagt gctggcaacc gagatctcaa aatcttcagt gcaatcggct
     1321 caatacaata ttgaaaaaaa tggcattgtt aacctacaaa ttatccgaat gtcggcagaa
     1381 gaatttaccc aagcgatgaa cggtgtgcga gccttttatc gcttaaaagg gattgattta
     1441 aacgcctacg aatgcaatac catttttgtt gatccacctc gtgcagggtt agatcaagct
     1501 acattaaata tggtgcaagc gtatgagcgg attctctata tctcttgcaa tccggaaacc
     1561 cttgccgaga atttacaaca attaagccaa acccatcgga ttgaaaaagc cgctcttttt
     1621 gaccaattcc cttacaccca ccatattgaa agtggcgttt ggttaattag aaaataaatt
     1681 gtgctgaaaa aaagaacgaa taagcagctt ttaagattta ttacttgacg atgttcgcaa
     1741 taatctctat tattgcaacg ttttcaacgg aggaatggca gagcggttga atgcaccggt
     1801 cttgaaaacc ggcgagggtt aacgccctcc gtgagttcga atctcacttc ctccgccagt
     1861 ctttaaacct gttgagcaat cagcaggttt ttttatttca tttgcaagaa aatcagataa
     1921 aaccaccgct tgaatccatc gctataaact ggttgaaatc agcctaaagt tgctttagga
     1981 taattgcgat tcattatcaa taacttaatt attatgaaat acctttatat tatctgtggt
     2041 tttatttgta ttggtttagg catcatcggg gcagtattac cgggcttgcc gaccacaccg
     2101 ttcttacttt tggctctgtt ctgctttact aaaggctcgg agagaatccg ccagtggttt
     2161 attcaaacca aattatacaa aaaatatctg caagaatatg atgagaaacg ggcaatgacg
     2221 atgaagcaaa aaatcacgat tttagcgatt tcgtttccgt ttacactatt tgctttcttt
     2281 gcactgcccc atatcggtgg gaaaattgcg ttagtagtgt tagtgataat tcaatattgg
     2341 tatttcttct gcaaaatgaa aacccttgaa ccggagaatg cctaatcttc ctgcttccgc
     2401 cttgctcttg ggtgggcaga gtcataaatt ttagctaagt gttggaaatc cagatgcgta
     2461 taaatttgag tggtggacag gctactatga cccaataatt cctgaaccgc tcgcaaatca
     2521 ccgcttgcct ctaacatatg agtcgcaaaa gagtgacgta atttatgtgg atgcagatgg
     2581 ctttccagcc cttgtcgctc accccatttc ttcattgcca actgaatgga acgatgagat
     2641 aaccgcccac ctcgcttatt cagaaacacc gcattatctt gcggcttaaa ttgcaaacgc
     2701 atttctaacc agcgatttaa cgcttttaac gcttgcgagc caatcggcac aattcgctct
     2761 ttgctaccct taccttgcac ccgtacttct cgtgccgata aatccatatc gcctaaatcc
     2821 agcccttgta attccgataa acgcaagccg gaactataca tcagctccat catcgcaata
     2881 tctcgaatat cgctcggctc atcactctca atcgccagca actgcccgac ctgctccgca
     2941 tcaatatttt tcggcaaatg cttacctact ttcggcgatt tcacccctaa agtcgggttt
     3001 gccgacattc gcccgatttt aatcaaatag acaaaccatt gccgtaacgc caccaagcgt
     3061 aagccgatac ttttcgcact caacccttgc ttatggcttt ggcttaacat ccaacgcacc
     3121 gagctacttt cgacctcttc ccaattttca atgcctgctt gcgaaaaaag ctcgctcacc
     3181 gccattaatt ggcgttgata attgcttaag gtatgcgggc taacttgctt ttcaatgcgt
     3241 aagaaatccc aataggggcg ggtttgttgg aaaagagggt tggaagtggt cataaacgta
     3301 ataacaagcg gttaattttt aggaaaatgt tgcaagggca aaagatttgc cctttgagat
     3361 taggctagcc ccaactgacc gatacgcacg ccaacattac caagtgcaac agggcttaaa
     3421 tattcgccga gaaattcaaa caattcattc acatcagtaa aactgcgatg agttttcact
     3481 tcttgctcca ctttacgagt aaattcataa tgtacatttt cgccgttaat aaaggcttcc
     3541 agcgttaaat atacggtttc aaaatagtgg cttggcgagc cttcaaagcc cggatcactc
     3601 actcgaacat tccaatgatt actaaataaa acttcggttt ttactgacat tgtgttttcc
     3661 ttcttcctat aagtacgtac attattttaa tcaagataga ttgaatttgc cacttttcct
     3721 agtcagaatc gaggcaaatc ttatttggtt tgtcctctta tatcttt
//
LOCUS       DANLXS010000212         3741 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.215, whole genome shotgun sequence.
ACCESSION   DANLXS010000212 DANLXS010000000
VERSION     DANLXS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.215"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(89..1024)
                     /gene="ftsX"
                     /locus_tag="PXD71_002392"
     CDS             complement(89..1024)
                     /gene="ftsX"
                     /locus_tag="PXD71_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDL6292456.1"
                     /translation="MIRSFSKLGAQTNYSLRAVWQDLMQRKLGTFLTVLVIAVSLTIP
                     TVSYLLWKNTHEAATQFYPEPQLTVYLHKNLAEHDVNTVVDRIRAFETDKIESFNYIS
                     RQQSLDEFRSWSGFGEALDILDENPLPAVVTLKPKANFNNADTMVELRNGLQQIKGVQ
                     EVRLDNGWLEKLTALTWLIARIAVVCTLLMLLAVFLVIGNSVRTDVANSKAAIEVQQI
                     LGATDHFIARPFLYTGMIYGLFGSLLAVLFSAVTISYFTGVVKYVTDMFTVQFELNGL
                     DFSEAFFLVVLCVFIGWLSAKIATMRNIFRIGSRG"
     gene            complement(1034..1687)
                     /gene="ftsE"
                     /locus_tag="PXD71_002393"
     CDS             complement(1034..1687)
                     /gene="ftsE"
                     /locus_tag="PXD71_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL6292457.1"
                     /translation="MIKFTNVNKVYKGGKPALQGIDFHLPAGGMAYITGHSGAGKSTL
                     LKLIMGIERANGGQILFNGHDITRLAHHELPFLRRQIGMVHQDYRLLTDRTILDNVAL
                     PLIILGMNQAIVEREARIALERVGLADKANYLPLHLSGGEQQRVDIARAIVHRPTLLL
                     ADEPTGNLDEKLSFEIFRLFEEFNQAGTTVLIATHDTNIISKRPKPCLVLEQGHLKF"
     gene            complement(1680..3176)
                     /gene="ftsY"
                     /locus_tag="PXD71_002394"
     CDS             complement(1680..3176)
                     /gene="ftsY"
                     /locus_tag="PXD71_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL6292458.1"
                     /translation="MSEKKKGFWSWLGFGKKEEAEQKEIQQEQESIQPEQPVIVEPET
                     EKSFSEKLDELEDKIEEKVDQLEDYFEEKEEKIEQFAEQVEDTIEQKVEDFKEFVEEK
                     VEEAKAFFDDVEDKIEQTFEEQKGEQAVENLQNIATEQLDLNEQEQVTYQVEEAVAEE
                     VAETELVNNVQDTVEESTIQDEYQEKPSQGGFFSRLIQGLIKTKQNIGSGFRNFFRGK
                     KIDDELFEELEEQLLVADLGMPTTQKIINNLTQHATKQQLKDADLLYQQLKVELGEVL
                     KPVAQPLVLEAKKPYVILMVGVNGVGKTTTIGKLARKFQQEGKSVMLAAGDTFRAAAV
                     EQLQVWGERNNIPVVAQSTGSDSASVIFDAMQSAAAKGIDVLIADTAGRLQNKNNLMD
                     ELKKIVRVMKKYDETAPHEIMLTLDAGTGQNAISQAKLFNEAVGLTGITLTKLDGTAK
                     GGVIFAIADQFNIPIRFIGVGEKIEDLRPFNAEEFIEALFSHEDEQND"
     gene            3255..>3741
                     /gene="rsmD"
                     /locus_tag="PXD71_002395"
     CDS             3255..>3741
                     /gene="rsmD"
                     /locus_tag="PXD71_002395"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL6292459.1"
                     /translation="MKKNRNSPQSVQQMGEVRVIAGLWRGRKLPVLNAEGLRPTTDRV
                     KETLFNWLMHDIAHSRCLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANQLKKNLQA
                     LKSEQGSVIHTDTLAYLAQKNNGEPFNIVFIDPPFHQGFVPQVLNLLAENNWLAPNAI
                     LY"
BASE COUNT         1037 a          905 c          671 g         1128 t
ORIGIN      
        1 agcaataata tattgcttat agtaaaaaac caagttccta ggaactggtt tttatctgca
       61 attatttctc taaaaacaac cgcttgtatt agcctctact accaatacgg aaaatatttc
      121 gcattgtcgc aatttttgcc gacagccagc caataaacac acaaagcacc actaagaaga
      181 aggcttcgct gaaatctaat ccgttcagtt caaattgtac ggtaaacata tcagtcacat
      241 acttcactac accggtgaaa tagctaatcg ttaccgcact aaacagcacc gctaacaggc
      301 tgccaaacaa gccgtaaatc atacctgtgt ataaaaacgg acgggcaata aagtgatcag
      361 ttgcccctaa aatttgttgg acttcaatcg ctgctttgct attggcaaca tcggttcgca
      421 cactgtttcc aatcactaaa aaaactgcca acagcattaa taaggtacac accaccgcaa
      481 tacgagcaat cagccaagtt agagcagtta atttttccag ccagccgtta tctaaacgaa
      541 cctcctgcac ccctttaatt tgttgcaaac cgttgcgtag ctccaccatc gtatcagcat
      601 tattaaaatt tgctttcggt tttaaggtaa cgaccgcagg caacgggttt tcatctaaaa
      661 tatctaacgc ttcaccaaag cctgaccaag aacgaaattc gtctaaactt tgttggcgag
      721 aaatatagtt aaatgactca attttatccg tctcaaaagc acgaatgcga tcaacaacgg
      781 tattcacatc gtgttctgcc aaatttttgt gcagataaac ggttaattgt ggctcagggt
      841 aaaattgtgt tgctgcctcg tgggtattct tccaaagtaa ataactcacg gttggaatcg
      901 taagtgagac cgcaatcacc agcaccgtca gaaaagtgcc gagcttgcgt tgcattaaat
      961 cttgccaaac cgctcttaaa gagtagttgg tttgagcacc taatttacta aatgaacgaa
     1021 tcataatatt tccttaaaat ttaagatgcc cttgctccag cactaaacac ggctttggac
     1081 gctttgaaat aatattggta tcgtgggtgg caatcagcac cgttgtgccg gcttgattaa
     1141 attcctcaaa caaacggaaa atttcaaacg agagcttttc atctaagttt ccggttggct
     1201 catcggctaa taacagcgtt gggcgatgaa caatggccct ggcaatatcg acacgttgtt
     1261 gctcaccgcc ggaaaggtgc aacggcagat agttcgcttt atctgccaag ccaacacgct
     1321 caagggcaat gcgggcttca cgctccacaa tcgcttggtt cattcccaaa ataatcaatg
     1381 gtaatgcgac attatccaaa atggttcggt cggttaataa gcgataatct tgatgcacca
     1441 tcccgatttg gcggcgtaaa aacggcaatt catgatgagc aagtcgggta atatcgtgtc
     1501 cgttaaacaa aatttgcccg ccattggcac gctcaatccc cataattaat tttaatagcg
     1561 tgcttttccc cgcaccggaa tgcccggtaa tatatgccat tccaccggca ggtaggtgga
     1621 aatcaatgcc ctgtaaggct ggctttccgc ccttataaac cttgttcaca ttagtgaatt
     1681 taatcattct gttcgtcctc gtggctaaat aaggcttcaa taaattcttc tgcattaaac
     1741 gggcgtagat cttcaatttt ctcgcccacc ccaataaagc ggatcggaat attgaattga
     1801 tcggcaatcg caaaaatcac gccacctttt gccgtaccgt ctaatttagt taaggtgata
     1861 ccggttaaac ccactgcctc attaaacaat ttcgcttggc tgatcgcatt ttgcccagtg
     1921 ccggcatcaa gcgtaagcat aatttcgtgc ggtgcggttt catcatattt tttcatcacc
     1981 cgcacaattt tcttcaactc gtccatcagg ttatttttgt tttgcaaacg ccctgcagta
     2041 tcagcgatta gcacgtcaat cccttttgcc gcagcggatt gcatcgcatc aaaaatcacc
     2101 gaggcagaat ccgaaccggt gctttgtgcc accaccggaa tgttatttcg ctctccccaa
     2161 acctgtagct gttcaactgc cgccgctcgg aacgtgtctc ctgctgctaa cattaccgat
     2221 ttgccttctt gttggaattt acgggcaagt ttgccaatgg tagtggtttt ccccacgcca
     2281 ttcacaccaa ccattaaaat gacatacggt tttttcgctt ccaacactaa cggttgagcc
     2341 accggtttta gcacttcacc cagttccact ttcaactgtt gatacaacaa atctgcatct
     2401 tttaattgtt gcttggtagc atgttgcgtt aaattgttga tgattttttg tgtggtcggc
     2461 atacctaaat ccgccacaag caactgctct tccagctctt caaataactc atcatcaatt
     2521 ttcttgccac ggaagaaatt acggaagccc gaaccaatat tctgcttggt tttaatcaag
     2581 ccttgaatta aacgactgaa gaaaccgcct tgactcggtt tttcttgata ttcatcttgg
     2641 atagtagatt cttcaactgt gtcttgaaca ttatttacta attcagtttc agcaacttct
     2701 tcagccacag cctcttcgac ttgataagta acttgctctt gttcgttgag atctaactgc
     2761 tcagttgcaa tattttgaag attttcgacc gcttgttcgc ctttttgttc ttcaaatgtt
     2821 tgttcgattt tatcttcaac atcatcaaag aacgctttcg cctcttcaac tttttcttca
     2881 acaaactctt tgaaatcttc gactttttgt tcaatggtat cttctacttg ctcagcaaat
     2941 tgctcaattt tttcttcttt ttcctcaaaa tagtcttcta actgatctac cttttcttca
     3001 attttatctt ccagctcatc taatttttca gaaaacgact tctcagtttc cggttcaaca
     3061 ataacaggct gttccggttg aatactctct tgttcttgtt gaatctcttt ctgttcagct
     3121 tcttctttct taccaaatcc aagccaagac caaaaccctt ttttcttctc tgacatattt
     3181 ttctctcatt tctcgtaaaa atagactaaa cttacatagt ctatcaaaaa atcccttatt
     3241 ttcctttagt gagtatgaaa aaaaatcgaa attcccccca atctgttcag caaatgggcg
     3301 aagtacgggt tatcgccgga ctgtggcgag gcagaaaatt acctgtacta aatgccgagg
     3361 ggcttcgccc gacaaccgat agagtaaaag aaacgctgtt taactggtta atgcacgaca
     3421 ttgctcactc acgctgtctt gattgctttg caggcagtgg ctctctgggc attgaagccc
     3481 tttcccgcca agcacaagcg gtcgtttttt tggaaaaatt tgcaaatgct gccaaccagt
     3541 taaagaaaaa tctccaagca ttaaaatccg aacaaggtag cgttattcac actgacaccc
     3601 tcgcctattt agcacaaaaa aataacggtg agccatttaa catcgtattt atcgatccgc
     3661 cttttcatca agggtttgta cctcaagtgc taaacttgct ggcagaaaat aactggctcg
     3721 ccccgaatgc gattttgtat g
//
LOCUS       DANLXS010000213         3633 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.217, whole genome shotgun sequence.
ACCESSION   DANLXS010000213 DANLXS010000000
VERSION     DANLXS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.217"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(108..230)
                     /locus_tag="PXD71_002396"
     CDS             complement(108..230)
                     /locus_tag="PXD71_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HDL6292460.1"
                     /translation="MKKLVLTILAAATFLTACNTVDGVGEDVEKAGDKIQQAAQ"
     gene            complement(310..1287)
                     /gene="rluD"
                     /locus_tag="PXD71_002397"
     CDS             complement(310..1287)
                     /gene="rluD"
                     /locus_tag="PXD71_002397"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDL6292461.1"
                     /translation="MTQQKILTAEITADLLGARLDQALAQLFPDYSRSRIKVWIESDL
                     VKVNGNIVNKAREKVFGGETVEITAEIEEEIRFEPQDIPLNIVYEDDDIIVINKPKDL
                     VVHPGAGNPDGTVLNALLHYYPPIAEVPRAGIVHRLDKDTTGLMVVAKNIPAQTHLVT
                     ALQKRRITREYEAIASGIMTQGGKVDEPMARHPTKRTAMAVHPMGKPAVTHYRIMERF
                     RNYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQLYGGRPRPPKGASDSFLSVLRGFQR
                     QALHATMLRLEHPITGEMMEWHAPLPDDFIELIQALKADYELYKDQLDY"
     gene            1428..2207
                     /locus_tag="PXD71_002398"
     CDS             1428..2207
                     /locus_tag="PXD71_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDL6292462.1"
                     /translation="MRKFKSLATLVLAGLFVAGCSNSNKELEQSNVQDLYGKGQTYLQ
                     DGDYNSAIRYLEAVGTKGGQYSGLGEQTQLSLIYAQYKVGEYYKALDAAERFARSYPN
                     AANMDYVFYLAGLSNARLSDNFIQDFFGVNRASRAVDNVRNAYGNFQTIVQRYPQSQY
                     AQDAQNWMNYLFNRLAEHELAVVKFYDERNAYVAVVNRIEEMLRFYPNTLATKQALPY
                     LKEAYKQMNIPDAEQKTELLIQEYESKELPNPEKLKYGEQF"
     gene            2280..3311
                     /gene="tsaD"
                     /locus_tag="PXD71_002399"
     CDS             2280..3311
                     /gene="tsaD"
                     /locus_tag="PXD71_002399"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL6292463.1"
                     /translation="MRILGIETSCDETGVAIYDEDKGLVANQLYSQIDMHADYGGVVP
                     ELASRDHIRKTLPLIQEALKEANLQPSDIDGIAYTAGPGLVGALLVGSTIARSLAYAW
                     NVPALGVHHMEGHLLAPMLEENAPEFPFVALLISGGHTQLVKVDGVGQYELLGESIDD
                     AAGEAFDKTGKLLGLDYPAGVAMSKLAESGTPNRFKFPRPMTDRPGLDFSFSGLKTFA
                     ANTIKANLNENGELDEQTKCDIAHAFQQAVVDTILIKCKRALEQTGYKRLVMAGGVSA
                     NKQLRADLAEMMKKLKGEVFYPRPQFCTDNGAMIAYTGFLRLKNDEQTDLSISVKPRW
                     AMTELPPIN"
     gene            3393..>3633
                     /locus_tag="PXD71_002400"
     CDS             3393..>3633
                     /locus_tag="PXD71_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL6292464.1"
                     /translation="MNNYVISLTSAQERRKHIEAEFGKQNIPFQFFDAITPDLIKEKA
                     KAFNIDISNTNLTKGEIACALSHIALWHLAKQQNLD"
BASE COUNT         1105 a          761 c          702 g         1065 t
ORIGIN      
        1 agagtaagcg gtaaaattta ccgtattttt cgcaaaaata taccttattg aacaattgtt
       61 caataaggta tatatattat tagcttctta gaattcgaag ctttttgtta ttgtgcagcc
      121 tgctgaattt tatcgcccgc tttttcaaca tcctcaccaa caccatcaac ggtattacac
      181 gcagtcaaga aagttgcagc tgctaaaata gttaatacta attttttcat agaaagctcc
      241 ttttttgttg tttacttatg aaaacgattt caatatagca actttacatt atttaagcaa
      301 cacctaaact taataatcta attgatcttt ataaagctca taatccgctt ttagtgcctg
      361 aatcaattca ataaaatcat caggtaacgg ggcgtgccac tccatcattt caccagtaat
      421 cggatgctct aatctcaaca ttgtggcgtg taaggcttgg cgttggaagc ctcgtaacac
      481 cgataaaaac gagtcactcg cacctttagg tgggcgaggt ctaccgccat agagctgatc
      541 acctaataat gggtgagcaa tatgcgccat atgtacccga atttggtgag tacgccctgt
      601 ttctaatctt aaacgtaaac gtgtgtaatt acgaaaacgc tccataatac gataatgcgt
      661 aaccgccggt tttcccattg ggtgaacagc cattgccgta cgtttagttg ggtggcgagc
      721 cattggctca tcaacttttc cgccctgtgt cataatgccg ctggcaatcg cttcatattc
      781 acgggtaata cgacgttttt gtaatgcggt tactaagtga gtctgtgccg gaatgttttt
      841 agccactacc attaagcctg tcgtgtcttt atccaatcgg tgaacaatcc ctgctcgagg
      901 cacttctgca atcggtggat aataatgtag caaagcattc agcactgtac cgtccggatt
      961 accagcccct gggtgaacca ctaaatcctt cggtttatta atgacgataa tatcgtcatc
     1021 ttcatacaca atatttagag gaatatcttg aggctcaaaa cgaatttcct cttctatttc
     1081 agcggtaatt tcaacagttt caccaccaaa tactttttcc cttgctttat tcacgatatt
     1141 gccattaact ttcactagat cactttcaat ccacacttta atgcgtgaac gagaatagtc
     1201 cggaaaaagt tgtgccagag cctgatctag gcgagcacct agtaaatctg cagtaatttc
     1261 tgctgttaaa atcttttgtt gagtcattaa accataaatc ctaattagtc atttgcttac
     1321 ttgcgtgatg ttatatagaa tagcgagatg aacttcataa gttattctta gtcagaggac
     1381 attgtacatt aacttttcgt cctagacact ttttataggt aaaacatatg cgtaaattta
     1441 aatctcttgc cacattagta ttagccggac tgtttgttgc aggttgttca aattcaaata
     1501 aagaattaga acaatccaac gttcaagatc tttatggcaa aggtcagacc tatttacaag
     1561 atggagatta taattctgcc attcgctact tagaagcggt tggaaccaaa ggaggccaat
     1621 attcaggact tggtgagcaa acccaactaa gtttaattta tgctcaatat aaagttggcg
     1681 aatactataa agcacttgat gccgcagaac gttttgctcg ctcttacccg aatgcagcta
     1741 atatggatta cgtattctat ctagcaggat tatcaaatgc acgtctaagt gataatttta
     1801 ttcaagactt ctttggtgta aatcgcgcct ctcgagccgt agataatgtt cgtaatgctt
     1861 acggtaactt ccaaactatt gtacagcgct accctcaaag ccaatatgca caagatgctc
     1921 aaaattggat gaattactta tttaaccgtt tagcggaaca cgaattagcc gttgttaaat
     1981 tttatgatga acgtaatgcc tatgtggctg tagtaaaccg tatcgaagaa atgttgcgtt
     2041 tttacccgaa tacactggca accaaacaag cattgccata cttaaaagaa gcatataagc
     2101 aaatgaatat cccagatgca gagcaaaaaa cggaactctt gatccaagaa tatgaaagca
     2161 aagagctacc gaatcctgaa aaactgaagt atggcgaaca attctagtcg tacagagaat
     2221 aatgtgaggg gcgttcttcg ccccttttgg ttttctaact tattttgact tctccaacta
     2281 tgcgaatttt aggtattgaa acctcttgtg atgaaaccgg tgttgccatt tatgatgaag
     2341 acaaaggctt agtggcaaac cagctttata gccaaattga tatgcacgcc gattacggtg
     2401 gcgtagtccc tgaactggct tctcgagacc atatccgtaa aacgttgcca ctaattcaag
     2461 aagccttaaa agaggccaat ctgcaaccct cggatattga cggcattgcc tatactgccg
     2521 gcccaggctt ggtcggggct ttattggtcg gctcaaccat tgcccgttcg ctggcttatg
     2581 cttggaatgt tccggcattg ggcgttcacc atatggaagg gcatttactt gccccaatgt
     2641 tggaagaaaa tgcccctgaa tttccgtttg tggcattatt gatttcaggt ggacacaccc
     2701 aactggtaaa agttgacggc gttgggcaat acgaactact cggggaatca attgatgatg
     2761 ctgccggtga agcctttgac aaaacaggca aactactcgg tttggattac cctgccggtg
     2821 tagcgatgtc aaaattagcc gaatccggca cgccaaatcg ttttaaattc cctcgtccaa
     2881 tgaccgacag accgggactg gatttcagtt tctccggttt aaaaaccttt gctgcgaata
     2941 cgattaaagc caatcttaat gaaaatggtg aactcgatga gcaaaccaaa tgcgatattg
     3001 cccacgcatt ccaacaagcc gtggttgata ctattttaat taaatgcaag cgagcgttag
     3061 agcaaaccgg ctataaacgc ttagtaatgg caggcggcgt aagtgccaat aaacaattac
     3121 gagcagacct tgcggaaatg atgaaaaaat taaaaggcga agtattctac cctcgcccac
     3181 aattttgcac tgacaacggc gcaatgattg cctacactgg ctttcttcgc ttaaaaaacg
     3241 atgaacaaac cgacttaagc attagcgtaa aaccccgctg ggctatgacc gaattaccac
     3301 cgattaatta acctttcaag cggtgaaatt tcttgttaat tttgcaaaaa tttaatcaaa
     3361 aataaccgct tgctatatga tagattaaat ttatgaataa ttatgtaatt agcctaacct
     3421 ccgcacagga gcgtagaaaa catattgaag ctgaattcgg caaacaaaat attccttttc
     3481 aatttttcga tgcgattacg ccggatttaa ttaaagaaaa agccaaagca tttaatattg
     3541 acatttcaaa tacaaactta acaaaaggtg aaatagcctg tgctttgagt catattgcat
     3601 tatggcattt agccaaacaa cagaatttgg att
//
LOCUS       DANLXS010000214         3631 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.218, whole genome shotgun sequence.
ACCESSION   DANLXS010000214 DANLXS010000000
VERSION     DANLXS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.218"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            13..396
                     /locus_tag="PXD71_002401"
     CDS             13..396
                     /locus_tag="PXD71_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292465.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEEDQIRCPKTYAKL
                     REILEANEENVVRIGNVSISIGSTT"
     gene            446..667
                     /locus_tag="PXD71_002402"
     CDS             446..667
                     /locus_tag="PXD71_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL6292466.1"
                     /translation="MNIYTDFLSREEIEFITQCKQKNLIKKQLNIMGIPFKENANGFP
                     VVRRDYAQAKQRKPIATNDSDWVSNAFKA"
     gene            689..1729
                     /locus_tag="PXD71_002403"
     CDS             689..1729
                     /locus_tag="PXD71_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6292467.1"
                     /translation="MARPRKYENNGLPQNLLCRRRKRANGSIVEYYFYVLADGKERSL
                     GTNKHEAILEAAKLNFENSRKSPIVLFIDVAKRYELEVVPTKKAKNTRQSNLQAIHWL
                     CKFFGNPPAPLEKIEPKHISQYLQWRKDTPAVANIEVGLFNTIWNMAREWGYTALPSP
                     SQGVKKFPTKYREVYVEDYILDKIYEFADERMADIIETAYLLGQRPIDICNIHRSHIY
                     DGILHITQQKTGKKVRFEISGRLKEILDKRLGYESDWIFTNKWGKKLKRRSLGDHFKE
                     IRERAMKAYPELADEIAKVQMRDMRAKAATDISLMTTDEQAQKQLGHTSKRMTHHYIR
                     KDKILKPTDEIT"
     gene            complement(1888..2175)
                     /gene="rplY"
                     /locus_tag="PXD71_002404"
     CDS             complement(1888..2175)
                     /gene="rplY"
                     /locus_tag="PXD71_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDL6292468.1"
                     /translation="MSFKFEAEVRSAQGKGASRRLRHNGQVPAIIYGGNAEPVSIILD
                     HDKVNNAQVHDSFYNEVLTIVVAGKEEQVKVQAIQRHPTKPKLVHLDFKRA"
     gene            complement(2327..3595)
                     /locus_tag="PXD71_002405"
     CDS             complement(2327..3595)
                     /locus_tag="PXD71_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HDL6292469.1"
                     /translation="MSDLKQQALDFHEFPIPGKISVTPTKALDTQHDLALAYSPGVAE
                     PCLAIEKDPSASYRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAG
                     IDVFDIEINETDPEKLVEIIASLEPTFGGINLEDIKAPECFYVEQKLRERMGIPVFHD
                     DQHGTAIITGAAVINGLQVVGKAISEVRLVVNGAGASAIACTNLLRSLGVKKENITMC
                     DSKGVIFQGRGDKMDETKQFYAIEDNGWRTLADAVANADVFLGCSKGGALTQEMVKTM
                     AKDLLILALANPVPEITPAEAKAVRPDAIVCTGRSDFKNQVNNVLCFPFLFRGALDVG
                     AKTINEEMKMAAAHAIAELAKEPVPFEVISTYGELSFGPEYIIPTPFDPRLIVNVSIG
                     VAKAAIQSGVATRPITDWEAYAEQLKKLVA"
BASE COUNT         1217 a          738 c          681 g          995 t
ORIGIN      
        1 tttggaggaa aaatgcacat agaagacaaa atcgcttggt ggcttgccaa tggagagaca
       61 ggggtaagca gtaaaacaat ggctttttac cttggttacg gaataagacc aaaaatagaa
      121 ggctacccac acgatgtatc ggatttcagg cgttgctttc tgttgttgga gacagtccct
      181 tttttgcgaa ataggattga aaaaatggct gaacttggga aagtttgggc tgcacttgca
      241 aaagaatggc acacattaga ggctctctac aacgaagagg aagatcaaat aagatgcccc
      301 aaaacctatg ctaagttgag agaaatccta gaagccaacg aagaaaatgt tgtccgcatc
      361 ggtaatgtga gcatttcgat aggttcaacg acttaacgat tatttaacca aatccctcta
      421 aatgaggggt ttatttggag agaatatgaa catctacact gacttccttt ctcgtgaaga
      481 gattgaattt attactcaat gcaagcagaa aaatctaatc aaaaaacaac tcaacattat
      541 gggcattcca tttaaagaaa atgcaaatgg attccctgtt gtccgccgtg attatgctca
      601 ggctaaacag cgtaaaccta ttgcaacgaa tgattctgac tgggtatcaa atgcatttaa
      661 agcgtaaatt gagaagaagg aggcacttat ggcacgccct cgaaaatatg aaaacaacgg
      721 attgccacaa aacctactct gccgccgcag aaaacgagca aacggaagta tagttgaata
      781 ctacttttac gttcttgccg acggcaaaga aaggtctctc ggtacaaaca aacacgaagc
      841 aattttagaa gcagccaagc taaattttga aaatagtaga aaatccccaa ttgttttatt
      901 tattgatgtg gcaaaacgct atgaattgga agttgttcca accaaaaaag ccaaaaacac
      961 tcgtcaatct aatttacagg ctattcattg gctatgtaaa ttctttggta acccacctgc
     1021 accgctcgaa aaaattgagc caaagcacat cagtcaatat ttacaatgga gaaaagacac
     1081 gccggctgtt gctaatattg aggtaggttt atttaacaca atttggaata tggcaagaga
     1141 atggggatac actgctcttc caagcccatc acaaggtgta aaaaaattcc cgaccaaata
     1201 tcgtgaagtt tatgtagagg attatatcct tgataaaatc tatgaatttg cagatgaaag
     1261 aatggcggat attatcgaaa cagcctattt gctgggacaa agaccgattg atatttgtaa
     1321 tatacaccgc tcacacatct atgacggtat attgcatatt actcaacaga agacagggaa
     1381 aaaagtgaga tttgaaatta gtggtcggct aaaagaaatt ttagataaaa ggttgggata
     1441 cgaatcagac tggattttta ccaataaatg ggggaaaaaa ttaaaacggc gaagtttagg
     1501 cgatcatttt aaagaaataa gggagagagc aatgaaagca tatcctgaac ttgctgatga
     1561 aatcgctaaa gtacaaatgc gtgatatgcg tgccaaagca gccaccgata tttccctaat
     1621 gacaaccgat gaacaagctc aaaaacaact tgggcataca tcaaaaagaa tgacacatca
     1681 ttacatcaga aaagacaaaa tcctcaaacc taccgatgaa atcacctaaa aataaagccc
     1741 ttttgccaaa tacaaaagag ccatttactc acctaaaatc cgttccgaaa ctattttgta
     1801 aataattgat tttattgacc taaaaaacta taaaatcgca aaaagtttcg gaacagaaaa
     1861 cagcgttaaa cccttgtaaa tcaaggatta agcacgtttg aagtctaagt gaactaattt
     1921 tggtttagtt gggtggcgtt gaattgcttg tacttttact tgctcttctt tacctgcaac
     1981 aacgatagtt aatacttcgt tatagaatga atcgtgaact tgtgcgttgt tcactttatc
     2041 gtgatctaaa atgattgata caggctctgc attaccaccg tagatgattg caggaacttg
     2101 accattgtga cgcaggcggc ggctcgcacc cttaccttgc gcagaacgaa cttcagcttc
     2161 aaatttgaat gacatagttt tattctctta aataaaatgg ggaaatcccc gtgaatagaa
     2221 aaaacagcag gcgacccagc tgtttccgaa aaaatatcgg catatccgtc tcgatatgcc
     2281 aactacgtat tttactgaag ttgcagaaaa atgcaatgta aaaatattaa gcaaccagtt
     2341 ttttcaactg ctctgcataa gcttcccaat ctgtaattgg tcgagttgct acacctgatt
     2401 gaattgctgc ttttgctaca ccaatcgaga cattcacgat caaacgtgga tcaaatggcg
     2461 ttggaataat atattccggg ccaaacgata attcgccata agttgaaata acttcaaacg
     2521 gcacaggttc tttagccaac tctgcaatcg catgggctgc tgccattttc atttcttcat
     2581 taatggtttt tgccccaaca tctaatgctc cacggaataa gaacgggaaa caaagcacat
     2641 tattcacttg gtttttaaag tctgaacgac cggtacatac aatcgcatct ggacggacag
     2701 ccttcgcttc agcaggtgta atttccggca cagggttcgc taaagcaaga attaatagat
     2761 cttttgccat tgttttaacc atttcttgcg ttaaagctcc gccttttgaa caacctaaga
     2821 aaacatctgc attcgcaacg gcatctgcca atgttctcca accattatct tctattgcat
     2881 agaattgttt ggtttcatcc atcttatcac cacgaccttg gaaaataacg cccttagaat
     2941 cgcacattgt gatattttct tttttcacac ctaatgaacg gagtaagtta gtacacgcaa
     3001 tcgctgatgc tccagcaccg tttaccacta aacgtacttc tgaaattgct ttacctacca
     3061 cttgtaatcc gttaatcacc gccgcaccgg taataattgc cgtaccgtgt tgatcatcgt
     3121 ggaacacagg gattcccata cgttcacgta atttttgctc tacataaaaa cattcaggtg
     3181 ctttaatatc ttccagatta ataccaccaa aagtcggttc taacgatgca ataatctcca
     3241 ctaatttctc cggatcagtt tcattaattt caatatcaaa cacatcaatt ccggcaaatt
     3301 tcttaaatag aacacctttg ccttccatca caggcttact tgctaaagca ccaatattgc
     3361 ctaagcctaa cactgctgta ccattagaaa ttactgcaac taagttacct cgtgccgtat
     3421 aacgataaga agctgaagga tctttttcaa tcgctaagca aggctcagca accccaggag
     3481 agtacgctaa agctaaatcg tgctgagtat ctaatgcttt agtaggagta acggaaattt
     3541 ttccggggat tgggaactcg tgaaaatcaa gcgcctgttg ttttaaatcg ctcattttag
     3601 aaaccttata atctataaaa ataaaagtaa t
//
LOCUS       DANLXS010000215         3628 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.219, whole genome shotgun sequence.
ACCESSION   DANLXS010000215 DANLXS010000000
VERSION     DANLXS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.219"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..986)
                     /locus_tag="PXD71_002406"
     CDS             complement(<1..986)
                     /locus_tag="PXD71_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDL6292470.1"
                     /translation="MADSVKSHEQSSDEIDLIEIFRALWDKKWWIVITTFITTLLAGI
                     YAFTAKEQWTSKATVIAPKLTELGEYLNVRREYSRILRVDPVDPNALSASLFNNFNRL
                     AQSQDTKNEFFITSNVYKTLAEGKDEQGQRTVLSNLSNENTSFIIPDAKKDPNAIGRT
                     LSFSAETPMEAQDTLGKLVQFINSKAFAVELEDFLVDFQNVLTALQYEKSLIQEDLSV
                     NKSVQLENLNKAYETAQKAGIKEYSKAFNQSDASNIAAALSDTKISLSDSKLADSSYL
                     FMLGEKYLKAQIDVANEKGVVYPPRYYQIDYQLKQLEPLFEKTKTVKTQTFS"
     gene            1135..1476
                     /gene="erpA"
                     /locus_tag="PXD71_002407"
     CDS             1135..1476
                     /gene="erpA"
                     /locus_tag="PXD71_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDL6292471.1"
                     /translation="MSDIQIPLIFTDAAARKVKSLIEGEENPNLRLRVYITGGGCSGF
                     QYGFTFDDQVNEGDLTIENQSVGLVVDPMSLQYLIGGTVDYTEGLDGSRFVVNNPNAS
                     STCGCGSSFSI"
     gene            complement(1581..1904)
                     /gene="yidD"
                     /locus_tag="PXD71_002408"
     CDS             complement(1581..1904)
                     /gene="yidD"
                     /locus_tag="PXD71_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL6292472.1"
                     /translation="MVKTHSPEQKAGTTESINQPPRLSLLAKILLLPIYFYRYAISPL
                     IGPRCRFYPTCSTYAVEAIKIHGAIKGGYLAFKRILRCNPLSEGGEDPVPLCCKGNCK
                     HNDKK"
     gene            complement(1853..2227)
                     /gene="rnpA"
                     /locus_tag="PXD71_002409"
     CDS             complement(1853..2227)
                     /gene="rnpA"
                     /locus_tag="PXD71_002409"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL6292473.1"
                     /translation="MKKLNFSRELRLLAPVQFKAVFEEPLRASTPQMTLLARENNLEN
                     PRLGLTVAKKHLKRAHDRNRIKRIVRESFRLKQHELPNFDFVFVAKGGIGKLDNATLF
                     ETLEKLWSRHIRLSKKPAQQNP"
     gene            complement(2263..2397)
                     /gene="rpmH"
                     /locus_tag="PXD71_002410"
     CDS             complement(2263..2397)
                     /gene="rpmH"
                     /locus_tag="PXD71_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL6292474.1"
                     /translation="MKRTFQPSVLKRARTHGFRARMATKNGRQVLARRRAKGRKSLSA
                     "
     gene            2634..3596
                     /gene="hemH"
                     /locus_tag="PXD71_002411"
     CDS             2634..3596
                     /gene="hemH"
                     /locus_tag="PXD71_002411"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDL6292475.1"
                     /translation="MNQPQQIGILLANLGTPDEPTPSAVKRYLKQFLSDPRVIDLPKF
                     KWQAILNCIILPRRSPKVAKLYQSVWSEEGSPLLAISRQQQRAVQRYFDAKGKNVVVE
                     LGMSYGNPSIESATDNLIKQGVERIIVLPLYPQYSSTTTASVFDAFANGLKAQRKIVP
                     FDFIHSYHNDPLYIQALANSIDVKPDELLLFSFHGIPKRYETEGDFYAKHCQETAELV
                     AEKLGLPREKWYLSFQSRFGDEEWLQPYTDQTLESFPQKGIKKVAVVCPGFSADCLET
                     LEEITEENRENFEHAGGESYRYIPALNASGDHIKALVKLIEAKI"
BASE COUNT         1101 a          690 c          714 g         1123 t
ORIGIN      
        1 taactaaaag tttgtgtttt aactgtcttt gttttttcaa ataacggctc tagctgttta
       61 agttggtaat caatttgata ataacgtggt ggataaacaa cacctttctc attagccaca
      121 tcaatttgag cctttaggta tttttcccct aacataaata aatatgaact atctgctaat
      181 tttgaatcag aaagggagat tttagtatca cttaaagctg ctgcaatatt tgatgcatct
      241 gattgattaa atgcttttga atattcttta atacctgctt tttgtgcagt ttcataggct
      301 ttatttaagt tttccaactg aaccgactta ttcacagata aatcttcctg aattaatgat
      361 ttttcatatt gtaatgcagt aagcacattt tggaaatcga ctagaaaatc ttctaattca
      421 acagcaaatg ctttgctatt aatgaattga accaacttac caagggtgtc ctgcgcctcc
      481 ataggtgtct ctgcagaaaa agatagtgta cgacctattg cattaggatc tttctttgca
      541 tcaggaataa taaagctggt attttcatta ctcaaattgg ataaaactgt gcgttgcccc
      601 tgctcatctt ttccttcagc taacgtctta tacacattgg aagtgataaa aaattcattt
      661 ttagtatctt gagattgtgc taatcggtta aaattgttga acaaactcgc agacaatgca
      721 tttggatcaa caggatctac acgcaaaatc cgactatatt ctctgcgaac atttaaatac
      781 tcacctaact ctgttaattt aggggcaata actgttgcct tagacgtcca ttgctctttt
      841 gcagtaaatg cataaatacc agcaagtaat gtggtaatga atgttgttat aacaatccac
      901 cattttttat cccatagagc acgaaaaatc tcaattaaat caatctcatc cgagctttgc
      961 tcatgacttt ttactgaatc tgccatagtt tcctctaaaa tttgcatgtt tctgtacagc
     1021 attatacata attttcttat ttccactgca ttcatttact cttttttaag attgcacttc
     1081 ctatgtaatt attagataat atccaatcac agtaaaattt tgatctaaaa caaaatgagc
     1141 gatattcaaa ttcctttaat ctttactgat gcggcggctc gtaaagtaaa aagcttaatt
     1201 gagggcgaag agaacccgaa tcttcgcttg cgtgtgtata ttacgggtgg gggctgtagt
     1261 ggattccagt atgggttcac ctttgatgat caagtcaatg agggagacct taccattgag
     1321 aatcaaagcg ttggcttggt ggttgacccg atgagcctac aatatttaat tggtggcacg
     1381 gtggattata ccgaagggct ggacggctca cgttttgtcg tgaataatcc aaatgcaagc
     1441 tcaacctgtg gttgtggttc ttcctttagc atttaattca ctcgaattat tgccttattc
     1501 aatatcagtc tcacaagtaa attttttact aaaaatgacc gtttgtatta aacaaaatag
     1561 cctaacttat tgagtttagg ctatttttta tcattatgtt tgcagttgcc tttacaacaa
     1621 agtgggacag gatcttcgcc tccctcactc aaagggttgc aacgcagaat acgcttaaac
     1681 gctaaatacc cacctttaat tgctccgtgg attttaatcg cttcgaccgc ataggtggag
     1741 caggttgggt aaaatcggca acgtgggcca atcaatgggc tgatagcata gcgatagaaa
     1801 taaatcggta gcaatagaat tttggctaat aggctgagac gaggtggctg atttatggat
     1861 tctgttgtgc cggctttttg ctcaggcgaa tgtgtcttga ccatagtttt tccaatgttt
     1921 caaacagcgt tgcgttgtct aatttgccaa tgccgccttt tgccacaaat acaaaatcga
     1981 aattcggcaa ttcgtgttgc ttgaggcgaa aactttcacg cactatccgc ttaatgcgat
     2041 tacggtcgtg agcccgtttt aaatgttttt tggcaacagt taagcctaga cgaggatttt
     2101 ctagattatt ctctctagca agaagagtca tttggggtgt gctggctcga agtggctctt
     2161 caaacaccgc cttaaattga acgggagcta acaaacgtag ctcccgagag aaatttagct
     2221 tcttcacact taatcagaaa ggtgttttgg ctggttctgc aattatgcag ataaagattt
     2281 acgaccttta gcacgacgac gcgctaacac ttgacgaccg tttttagtag ccatacgagc
     2341 acggaaaccg tgagtacgag cacgttttaa tactgatggt tgaaatgtac gtttcattgc
     2401 ttatctacct aaaatttaaa aagttggcat aaaatggttt ctgccaaaca aggaccataa
     2461 aaaagagttg caattctaca ggcaattaaa ataaaaagca agaaacaatc caagcacaac
     2521 tcgggttgtt tgcaaaattt ttgaaaaata cgaccgcttg ttataatcgc agaaatttat
     2581 attggtttgt ggcataatcg aaaaaatatt aactattgat gagaaataga acaatgaatc
     2641 aaccccaaca aataggtatt ttgctggcta atttaggtac gccagatgaa cccacgccaa
     2701 gtgcggtgaa gcgttattta aagcaatttt taagcgatcc tcgtgtgatc gatctaccaa
     2761 aatttaaatg gcaagcaatc ctaaactgca tcattttgcc aagacgttcg ccaaaagtgg
     2821 caaaattata tcaatcagta tggagcgaag aaggctcacc gttattggcg atttctcggc
     2881 aacaacaacg agcggtacaa cgctattttg atgccaaagg caaaaatgtg gtggtggaat
     2941 tgggaatgtc ttacggcaat ccaagtattg aaagtgcgac cgataacctc atcaagcagg
     3001 gggttgagcg gattattgtg ctaccgcttt atccgcaata cagtagcact actaccgctt
     3061 cggtgtttga tgcgtttgcc aacggtttaa aagcacagcg taaaatcgtg ccgtttgatt
     3121 ttattcacag ctatcataac gatccgcttt atattcaagc gttggctaac tctattgatg
     3181 ttaaacctga cgagttatta ctgttttcat ttcacggcat tcctaaacgt tatgaaacgg
     3241 aaggtgattt ttacgccaag cattgccaag aaacagcaga attagtggct gaaaagctag
     3301 gtctgccaag agaaaaatgg tatttgtctt tccagtcccg ttttggtgat gaagagtggc
     3361 tacagcctta taccgatcaa acccttgaaa gcttcccaca aaaagggata aaaaaagtgg
     3421 ctgtcgtttg cccgggcttt tcagcagatt gtttggaaac cttagaagaa attacagaag
     3481 aaaaccgcga gaactttgaa catgccggcg gagaatctta tcgctacatt ccagcgttaa
     3541 atgcgagtgg cgatcatatc aaggcattag taaaattaat cgaagcgaaa atataatata
     3601 atttgggcgg ataataaatc cgcccatt
//
LOCUS       DANLXS010000216         3628 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.220, whole genome shotgun sequence.
ACCESSION   DANLXS010000216 DANLXS010000000
VERSION     DANLXS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.220"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(63..2627)
                     /locus_tag="PXD71_002412"
     CDS             complement(63..2627)
                     /locus_tag="PXD71_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HDL6292476.1"
                     /translation="MRIAKIIFSALLSLLVLGGVIGGLLYMHFKSDLPDVATLKNVEL
                     QQPMQIFTLDGKLIGEVGEERRIPVKLDDIPPMLVKAILATEDARFYEHKGIDPKGIM
                     RAIWRTSQGDTQGASTITQQLAKNFFLTPERSLERKAKEAILALEIEQVLSKNEILEL
                     YLNKIYLGYRSHGVAAAAKTYFNKSLKDLTLSEIAIIAGLPKAPSTMNPLYSVKRAED
                     RRNVVLGRMLETGDITKEEYEKAKAEPVVANYYGATLEFRADYVTEMVRQEIVKRYGE
                     NIAYNKGLKVYATVLSADQKAAQDALRENLIDYDRRHGWRGAEKLWTGSSAWDDEKII
                     DHLSKLPKSEPFTPAVVMQADKSKYTILLANGDISVLKRTNASFAPKGLKAGEQIWVR
                     QNKNREWALGQIPEVNSALVSINSENGAIESIVGGFSFEQSRFNRATQSLVQVGSAIK
                     PFIYTAAMNKGLSLSTTISDSPIVIKKKGQKEWRPKNADGVYGGPLRTRVALGKSKNM
                     VAIRILQMAGIDYVADYLQRFGFNRNQYVATESLALGAASFTPLEMARAYAVFNNGGY
                     LIEPYIIDRIIDAQGYELYQANPVVACNSCDSPVVYPEPKYFESVKIHDEAQVGVQTI
                     ANSSNNDDDDVVEDVGDSEPELQANNSEKNAPSLMAESSHHIKGLRYAPHVISDELAF
                     LMRSALATAITGEPEYGWRGTSYKMLNTIKRADVGGKTGTTNNAKATWYAGFGANIST
                     VVYVGFDDNKRVLGRGASGSSTALPAWTNYMKVALSDKPVEKSTLPKNIIEVNIDPNS
                     GFLGNGRKEYFIKGTEPTKRYIVERAYTEKAPQKTESAPLRLGLPPPGVLKSGELF"
     gene            2751..3449
                     /locus_tag="PXD71_002413"
     CDS             2751..3449
                     /locus_tag="PXD71_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HDL6292477.1"
                     /translation="MKLLKAHSKTPSNRIILGLSEDEQRYCLVKNKAEKFTVFWQAKP
                     ASIEELIGNLTPQEQAMMTLVRPIPYQYIWRKTVFMANTLNEAQLHRKVIHILKNEQP
                     LALDLLNIDYQRFPSSNDNLDKVVIYAVRKSYTENLSQFNSILDCELHCYARAIIHLT
                     QSQNNQPAPCFSFKQKYFQFTETELLILQTEPEDCIHLSDLKIDHLEIECEQEKQLYC
                     LALGASLWNGKVLI"
     gene            3425..>3628
                     /locus_tag="PXD71_002414"
     CDS             3425..>3628
                     /locus_tag="PXD71_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292478.1"
                     /translation="MEWQGIDLSANRKNNWFAGSDKGIKNVIYTLLLSALISLALIFL
                     YLQKNQEIQPLAQQLELVKTDILN"
BASE COUNT         1096 a          803 c          606 g         1123 t
ORIGIN      
        1 tattatcgca agaaatcccc ctatttttca acttttaaga gcattatcct tgtaataaat
       61 tattagaata attcaccgct ttttagcacc cctggtggag gtaatcctaa acgtaacggc
      121 gcagattctg ttttttgagg cgctttttca gtataagctc tctctacaat ataacgtttt
      181 gtaggctcag tacccttaat aaaatactct ttacgtccat ttcctaagaa accactatta
      241 gggtcaatat taacctcaat aatatttttt ggaagggtac ttttttcgac aggcttatct
      301 gacaatgcaa ctttcatata atttgtccaa gctggtaaag cagtactaga acctgaagca
      361 cctctaccta atactctttt attatcatca aagccaacgt acacaacagt agaaatatta
      421 gcaccaaatc ccgcatacca agttgccttg gcattattgg ttgtaccagt cttcccgccc
      481 acatccgctc gttttatcgt attgagcatt ttatagcttg ttcctcgcca gccatattca
      541 ggctcaccag taattgctgt agctaaagca ctacgcatta aaaaagcgag ttcatcacta
      601 ataacatgag gagcgtaacg taaccctttg atatgatgag aagattctgc cattaagctc
      661 ggcgcatttt tctcgctatt gttcgcttgt aattcaggtt ctgaatcgcc aacatcttcc
      721 acaacatcgt cgtcatcatt attcgaggaa ttcgctatag tttgtacacc gacttgagct
      781 tcatcatgaa ttttgactga ttcaaaatat ttaggctcag ggtaaacaac tgggctatcg
      841 caactattac aagctactac aggatttgct tgatataact cgtagccttg ggcatcaata
      901 atacggtcaa tgatatatgg ttcaatcaaa taacctccat tattaaatac tgcataagct
      961 cgagccattt ctaatggcgt aaaagatgca gcacccaatg caagagattc tgttgcaaca
     1021 tactgatttc ggttaaagcc aaaacgttgt aagtagtctg caacataatc aatccctgcc
     1081 atttgtaata tacgaatagc caccatattt ttcgatttac ctaatgcaac acgagttctc
     1141 aacgggccgc cataaacccc atcggcattt ttaggtcgcc actctttttg ccctttcttt
     1201 ttaatgacaa taggagaatc gctaattgtt gttgaaagac ttagcccttt attcattgcc
     1261 gctgtgtaaa taaacggttt aatcgcagag cctacctgaa ctaatgactg agtcgcacga
     1321 ttgaaacggc tttgttcaaa gctaaagcct ccaacgatag attcaatggc accgttttca
     1381 ctattaattg acactagggc tgaatttacc tccggaattt gtcctaaagc ccactcccta
     1441 tttttatttt gacgtaccca aatttgttca cctgctttta accctttagg tgcaaaagaa
     1501 gcatttgtac gttttaaaac agagatatca ccatttgcca ataaaattgt atattttgat
     1561 ttatctgctt gcattacaac agctggtgta aacggctctg attttggcag cttacttaaa
     1621 tgatcgataa ttttctcatc gtcccaagca gaactacctg tccataattt ttcagcgcct
     1681 cgccaaccat gacggcggtc ataatcaatc aaattttcac gtaacgcatc ttgtgctgct
     1741 ttttggtctg ctgaaagtac cgtggcgtac acttttaagc ctttgttata agcaatattt
     1801 tctccatagc gtttcacaat ttcttgacga accatttctg tcacataatc cgctcgaaat
     1861 tctaatgtcg caccatagta gttagctact actggctctg ctttagcttt ctcatattcc
     1921 tcttttgtaa tatctccggt ttctagcatt ctcccaagca ctacattacg acggtcttcc
     1981 gcccgtttta ctgaataaag tggattcatc gttgaaggcg cttttggaag ccctgcaata
     2041 attgctattt cagaaagggt taaatctttc aaagatttat taaaataagt tttagccgca
     2101 gcggcaacac cgtgagaacg ataccctagg taaattttgt ttaaatacag ctccagaatc
     2161 tcatttttac tgagaacctg ttcaatttct aaagctaaaa ttgcctcttt ggccttacgc
     2221 tctaaactac gttcaggtgt taagaaaaag tttttcgcca gctgctgggt gattgtactt
     2281 gcgccttgag tatctccctg gctagtgcgc catattgcac gcataatacc tttaggatcg
     2341 atccctttat gctcgtaaaa acgtgcatct tcggtagcaa gaatcgcttt tactaacatc
     2401 ggtggaatat catcaagctt gaccggaata cggcgttctt ctcctacttc accaattaat
     2461 ttaccatcta aggtaaaaat ctgcataggc tgctgtaact ctacattttt cagcgttgcc
     2521 acatcaggca aatcagattt gaaatgcatg tacagtaaac caccaattac cccacctaaa
     2581 accaagagtg aaagtaaagc actaaatatt atttttgcga tccgcatcga aaaaatctct
     2641 tttggttaat gacgaaacat aaatttaaag ccaatattag gtcactaagt ataagctaaa
     2701 aacgcttact tcgcataact aattgcaaaa ctttccttac tatacttact atgaaattac
     2761 ttaaagcaca ttcaaaaaca ccttcaaacc gtatcatctt aggcttaagt gaagatgaac
     2821 aacgttactg cttagtcaaa aataaagcag aaaaattcac cgttttttgg caagccaagc
     2881 ctgcgagcat cgaagagctt atcggtaatc tcaccccaca agaacaagca atgatgacgc
     2941 tggttcgccc gatcccttac caatacattt ggcgaaaaac cgtttttatg gctaacaccc
     3001 tcaatgaagc tcaattacac cgtaaggtaa ttcacatttt aaaaaatgag cagccacttg
     3061 cattggatct actcaatatt gactatcagc gatttccaag cagcaacgat aatctggata
     3121 aagtggttat ctacgccgtg cgaaaaagtt ataccgagaa tttgagccaa tttaacagca
     3181 tcttagattg tgagctgcat tgctatgcac gggctataat tcatttaaca caatcccaaa
     3241 ataatcagcc tgcgccctgt ttttcattta aacagaagta cttccaattt accgagacgg
     3301 aattactgat attgcaaaca gaacctgagg attgtattca tctatctgat ctcaaaattg
     3361 accatcttga aatcgagtgt gagcaagaga aacagctata ttgcctcgcc ttaggagcaa
     3421 gtttatggaa tggcaaggta ttgatttaag tgctaatcga aaaaataact ggtttgccgg
     3481 cagtgataaa ggcatcaaaa acgtgattta tacactttta ttatctgccc tcattagctt
     3541 ggctctgatt tttctctatt tgcaaaaaaa tcaagaaatt caaccgcttg cacagcaatt
     3601 agagctggta aaaaccgata tattaaat
//
LOCUS       DANLXS010000217         3612 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.221, whole genome shotgun sequence.
ACCESSION   DANLXS010000217 DANLXS010000000
VERSION     DANLXS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.221"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            129..1052
                     /gene="oppB"
                     /locus_tag="PXD71_002415"
     CDS             129..1052
                     /gene="oppB"
                     /locus_tag="PXD71_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDL6292479.1"
                     /translation="MLKFIFKRLLEAIPTLLILITFSFFLMRLAPGSPFTSEKAYPPK
                     VMANIEAKYHLNEPLYKQYFLYMENLSKGDFGPSFKYKDQSVNNLIASAFPVSIKLGI
                     TAFIFAVLIGLTAGTIAALKQNSKWDYLVMGFSMTGIIMPSFVFAPLLVLMFAIYLKW
                     LPAGGWNGGQLYYMVLPVLSLTISYVAGIARITRGSMIEVLHSNFIRTAKAKGLPMRK
                     IIFKHALRPALLPVITYLGPAFVGIITGSMVIESVFGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGTLTILFNAIVDILYAVIDPKIRYS"
     gene            1067..1966
                     /gene="oppC"
                     /locus_tag="PXD71_002416"
     CDS             1067..1966
                     /gene="oppC"
                     /locus_tag="PXD71_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDL6292480.1"
                     /translation="MQNSSKNQPLVEVTSTIQGRSLWQDARRRFFRNKAAVASLIILC
                     CIILFITFAPMLMPFSYEDTDWNMMGIAPDFESQHYFGTDSSGRDLLVRVAIGGRISL
                     MVGIAGALIAVLIGTIYGAISGYFGGKVDMVMMRFLEILSSFPFMFFVILLVTFFGQN
                     IFLIFIAIGMVAWLNLARIVRGQTLSLKNKEFVEAAIVCGVSRRQIIWRHIVPNVLGL
                     VVVYASLEVPSLILFESFLSFLGLGTQEPMSSWGALLSDGAAQMETSPWLLAFPAFFL
                     CLTLFCFNFIGDGLRDALDPKDK"
     gene            1976..3571
                     /locus_tag="PXD71_002417"
     CDS             1976..3571
                     /locus_tag="PXD71_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6292481.1"
                     /translation="MELLAVNNLDVYLKTDEELVHAVRNVSFSIEKGQTLAIVGESGS
                     GKSVTSMAIMQLLPKNIVSFGERSSIIFEEQNLLTLSEKAMQTVRGDRIGMIFQEPMT
                     SLNPYMPIGKQVAETVQTHNPQISNAEAEKMVLETLQKVKIPDAEKKMICYPHEFSGG
                     QLQRIMIAMAIINKPDLLIADEPTTALDVTTQAEILDLMHDLQRDMGMAIILISHDLR
                     LVHKYSDLVCVMQNGEIIERGETETVFTNPQHPYTVELLTPIPTNLKGELPPNAPTLL
                     QGEHIKVDYILKRSLFGKPKKVFNALKDISLHLKTGETLGIVGESGSGKSTLGRAIMQ
                     ILDYRGNITFAGKTLANLNKAERQALKKDMQMVFQDPFNSLSPRLTVGEIIAEGLTVH
                     CPQMSKTERREKVMKMLEEVNLNPAMINRYPHEFSGGQRQRIAIARAIILEPKFVLLD
                     EPTSALDRSTQITVIELLNRLQKKYGLSYIFISHDLAVIKALSDRVIVMSQGEIVESG
                     DVTQIFENPQQEYTKRLILASNL"
BASE COUNT          997 a          725 c          784 g         1106 t
ORIGIN      
        1 agctaaaaaa acaaccgctt aagaataatg aattcttata aaaatagcca taactaatca
       61 atatctttac aaagccgtta atttacgcta ttaattgcct accaatataa ctaaaaagag
      121 tggtgcttat gctgaagttt atttttaaac gtcttcttga ggcgattcca accctactaa
      181 ttctgattac cttttccttt ttcctgatgc ggcttgcccc gggtagccca tttaccagtg
      241 agaaagccta tccgccgaaa gtaatggcaa atatcgaggc aaaatatcac ctgaatgaac
      301 cgctgtataa gcagtatttt ctttatatgg aaaatctgtc aaaaggcgat ttcggtccat
      361 cttttaaata taaagatcaa tcggtaaata atctgattgc ttctgcgttt cctgtttcga
      421 taaaactggg cattactgcc tttatttttg cggtgcttat cggtttaacc gcaggaacta
      481 tcgctgcact taagcaaaat agtaaatggg attatttagt gatgggcttt tcaatgacgg
      541 gtattattat gccgagcttt gtctttgctc cgctgttggt actcatgttt gcgatttatc
      601 tcaaatggtt gcctgccggt ggttggaacg gtggtcagct ctactatatg gttttacctg
      661 ttctctcttt aaccattagc tatgttgccg gcattgcccg tattactcgt ggttcaatga
      721 ttgaggtgtt gcactctaat tttattcgca ccgctaaggc aaaaggcttg ccaatgcgta
      781 aaattatttt caaacacgct ttgcgtccgg cattgttgcc tgtgatcact tatttaggcc
      841 cggcttttgt tggcattatt accggctcga tggtaattga aagcgtgttt ggcttaccgg
      901 gaatcgggca gttgttcgta aacggagcat taaatcgtga ctattcatta gtattaagcc
      961 taaccatttt ggtaggtacg ctcaccattt tatttaatgc gattgttgat attttatatg
     1021 cggtcatcga cccgaaaatt cgttattcat aggagtaaag cggaaaatgc aaaattcttc
     1081 aaaaaatcaa ccgcttgttg aggttacctc aaccattcaa ggacgaagtt tatggcaaga
     1141 tgcacgccgc cgttttttcc gaaataaggc ggctgtggca agtttgatta ttctttgttg
     1201 tattattctt tttattacct ttgccccaat gctaatgccg tttagctatg aagataccga
     1261 ttggaatatg atggggattg ctccggattt tgaatctcaa cactatttcg gtactgattc
     1321 ttcagggcgt gatttgctgg ttcgagttgc aatcggtgga cgcatttcgt taatggtagg
     1381 gattgccggt gcattaattg cggtgcttat cggtacaatc tatggagcaa tttccggtta
     1441 tttcggcggt aaagtcgata tggtgatgat gcgttttctg gaaattttaa gctcattccc
     1501 atttatgttc ttcgtaattt tgctggttac gttcttcggg cagaatattt tcctgatttt
     1561 cattgcaatc ggtatggtag cgtggctgaa ccttgcccgt attgtacgtg gtcaaactct
     1621 ttcgcttaaa aataaagagt ttgtggaagc agcgattgtg tgtggcgttt cccgccgtca
     1681 aattatttgg cgacacattg tgcctaacgt tttaggctta gtggtggttt atgcctccct
     1741 tgaagtacca tctctgattt tgtttgaatc gttcttaagt ttcttagggc ttggtacaca
     1801 agagccgatg agcagttggg gggcattatt aagcgacgga gcggcacaaa tggaaacctc
     1861 gccttggcta ctggctttcc ctgccttttt cttatgttta acactgtttt gttttaattt
     1921 tattggtgat ggcttgcgtg atgccctcga tccaaaagat aaatagaggt ttagaatgga
     1981 attattagca gtaaataatc tcgatgttta tttaaaaacg gacgaagaat tagttcacgc
     2041 agtgcgtaat gtctcgttct cgattgaaaa agggcaaacc cttgcgattg tgggggaatc
     2101 gggttcgggt aaatccgtta cttcaatggc aattatgcag ttattgccta aaaatatcgt
     2161 ctcattcggt gaacgctcaa gcatcatttt tgaagaacaa aacttgctta ctctatctga
     2221 aaaagcaatg caaacagttc gaggtgatcg catcggtatg attttccaag agccaatgac
     2281 ctcactcaat ccttatatgc cgattggcaa gcaggttgct gagacggttc aaacccataa
     2341 tccgcaaatc agcaatgccg aagcggaaaa aatggtgttg gaaactttgc aaaaagtaaa
     2401 aattcccgat gcggaaaaga aaatgatttg ctatccgcac gagttttccg gcggtcagct
     2461 acaacgaatt atgattgcaa tggcaattat caacaaaccc gatttattga ttgccgatga
     2521 gccgaccacc gccctggatg tcacaaccca agcggaaatt ttggatttaa tgcacgactt
     2581 acagcgtgat atgggaatgg cgattatctt aatttcccac gatttacgct tggtgcataa
     2641 atacagtgac ttagtctgcg taatgcaaaa cggcgaaatt attgagcgag gcgaaacgga
     2701 aactgtgttc accaatccgc aacatcctta tacggtggag ctacttaccc cgattccaac
     2761 taatttaaaa ggggaattac cgccaaatgc accaaccttg cttcagggtg agcatattaa
     2821 ggtagattat attttaaaac gctcgctatt cggtaagccg aaaaaggtgt ttaatgcgtt
     2881 aaaagatatt tcattgcact tgaaaaccgg cgaaacgctc ggcattgtag gcgaatccgg
     2941 ctccgggaaa tctacccttg ggcgtgccat tatgcagatt ttagattatc gcggcaatat
     3001 tacatttgca ggtaagacac tggctaatct gaataaagcc gaacgtcaag ccctgaaaaa
     3061 agatatgcaa atggtgttcc aagacccgtt caactcactt tctcctcgct taacagttgg
     3121 ggaaattatt gctgagggct taaccgtaca ttgccctcaa atgagcaaaa ccgaacggcg
     3181 ggaaaaagta atgaaaatgt tggaagaggt taatctcaac cctgcaatga ttaaccgcta
     3241 tccgcacgaa ttttcgggcg gacaacgcca gcggattgcg attgcaaggg caatcatttt
     3301 agagccgaaa tttgtattgc ttgatgagcc aacctcagca cttgaccgct ctactcagat
     3361 cactgtaatt gaattgctta accgcttaca gaaaaaatac ggcttaagtt atattttcat
     3421 cagccacgat ttggcggtca tcaaggcact tagcgaccga gtgattgtaa tgagccaagg
     3481 tgagattgta gaaagcggtg atgtcactca aatttttgaa aatccacaac aagaatacac
     3541 caagcggttg attttagcct ctaatttgta accccttcca caaggtctta ttctaccaaa
     3601 aaacaagacc tt
//
LOCUS       DANLXS010000218         3601 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.222, whole genome shotgun sequence.
ACCESSION   DANLXS010000218 DANLXS010000000
VERSION     DANLXS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.222"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            38..400
                     /locus_tag="PXD71_002418"
     CDS             38..400
                     /locus_tag="PXD71_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL6292482.1"
                     /translation="MKPTNKADFQRVINAAGYSMKGLKAAYINEAAFRQEIWCATILF
                     PLGLILGETNIEKALLVGTVLLVLVTELLNSAVESVVDRIGADFHELSGRAKDIGSAA
                     VFMAMVLLAITWILILFC"
     gene            437..2080
                     /locus_tag="PXD71_002419"
     CDS             437..2080
                     /locus_tag="PXD71_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDL6292483.1"
                     /translation="MRLKKTLLSVALLSATSAMAYETGKTYRFTVLHTNDIHGHFWQS
                     DKGEYGLAAQKTLVDNIRKEVAEKGGSVLMLNAGDANTGVPESDMQNARPDIEGMNAI
                     GYDAMVLGNHEFDYPLQILSMQEKWAKFPFISANVINKKTDKPIVKPYVMLERGGLKI
                     AVIGLTTEDTAKLGNPDVTENAFFKNPIETAKNTLAEINQKEKPDVRIALTHMGYYFD
                     GKHGTNAPGDVTMARTLEKGAFDLIIGGHTHDTVCIDEKGQFKAKYTPGEECKPDFQN
                     GTWIVQAGEWGKFVGRADFEFKDGKATLVKYELIPVNLKKTIKKEDGTKEYRLYQPEI
                     KADEATYTLLKKYQDEGDKKLNIEVGRVKGLLDGKREHIRFQQTNLGRLIAQSQMERV
                     KADIGIMNSGGIRTSLEEGVITYKHILTVQPFGNMIATVDLKGQELLDTLTTIALKEV
                     DTGAYPQFAGISMVVDRTAKTISDVKIGGKPFDKEKTYKVSLPDYVAAGGDGYPLLKK
                     HPSYVNTGFIDAEMLKKFFEENKIIEADKYDPKNQIIFK"
     gene            complement(2287..3156)
                     /locus_tag="PXD71_002420"
     CDS             complement(2287..3156)
                     /locus_tag="PXD71_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL6292484.1"
                     /translation="MSSRGKILNTISSLQNSLTKTEKKIAAAILSQPELLSQCSLSEV
                     AKQLDVGEATFIRFCRTLGFKGYTDFKLELAIELATQNQDNRVLLDTDVSESDTSKDI
                     AEKLKVSLDNVIEETINLLDFNVLEKVVEELRKAKRIFLFGVGSSGLTAEDAKHKLMR
                     IGLQTDAVTNNHFMYMQAALVKEGDLVIGISHSGYSEEIVKSLRFSRANKATTVAITH
                     NLRSPVTEEANYVLINGNRQGHMQGDSIGTKMSQLFVLDLIYTLLVKAEPENALKQKQ
                     KTLNVILEQRVKG"
     gene            complement(3166..>3601)
                     /locus_tag="PXD71_002421"
     CDS             complement(3166..>3601)
                     /locus_tag="PXD71_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL6292485.1"
                     /translation="YLKSLPPVFARLCKKLKSLDLVNLELGWQELENGIRMNVMSFET
                     TPAEGKKAELHRKFIDIQLLIDGEEMIEYGLSQPDLSKYDEYRDEDDYQLTAEIDDKN
                     NLILKPNMFAIFLPYEPHKPGNSVNGNKTLKKLVVKVPVELI"
BASE COUNT         1156 a          690 c          650 g         1105 t
ORIGIN      
        1 aatagttctc taaactcttt tatttatttt atttactatg aaaccaacca ataaagcaga
       61 ttttcaacgt gtgatcaatg cagctggcta ctctatgaag gggttaaaag ccgcttatat
      121 taatgaagcc gcctttcgcc aagaaatttg gtgcgctact attttattcc ctctagggct
      181 tattctaggc gaaaccaata ttgaaaaagc attattagta ggcacagttt tacttgtttt
      241 agttacagaa ctactcaata gtgcggttga atctgttgtt gaccgcattg gggctgattt
      301 ccacgagctt tctggtagag ctaaagatat tggctctgca gctgtattta tggcgatggt
      361 tttactcgct ataacttgga tattaatttt gttttgttaa ctctattttt caaccaacac
      421 tcttactgag gtatttatgc gcttaaaaaa aacattactt tcggtagcac tacttagtgc
      481 aacatcagca atggcctacg agacaggtaa aacatatcgt tttacggttt tacatactaa
      541 cgatatacac ggtcactttt ggcaaagtga taaaggcgaa tacggtctcg ctgctcaaaa
      601 aacattggtt gataatattc gcaaagaggt cgcagaaaaa ggtggttcgg tattaatgct
      661 gaatgcaggt gatgcgaata caggcgtgcc ggagtcagat atgcaaaatg cacgtcctga
      721 tattgaagga atgaatgcga ttggttacga tgcaatggtt ctaggtaatc acgaatttga
      781 ttacccactg caaattctct ctatgcaaga aaaatgggct aaattcccct ttatttcggc
      841 caatgtcatt aataaaaaaa cggataaacc cattgtaaaa ccttacgtga tgttagaacg
      901 aggcggacta aaaattgctg ttatcggatt aacaaccgaa gataccgcaa aattaggtaa
      961 tccagatgta acggaaaatg cctttttcaa aaaccctatt gaaacagcaa aaaacacatt
     1021 agctgaaatt aatcaaaaag aaaaacccga cgtacgtatc gctttaaccc atatgggtta
     1081 ctattttgac ggtaaacatg gtactaatgc accaggtgat gtaacaatgg cccgtacctt
     1141 agaaaaagga gcgttcgatt taatcattgg cggtcatacc cacgatactg tttgtattga
     1201 tgaaaaaggt caattcaaag ccaaatatac gccgggtgag gaatgtaaac cagacttcca
     1261 aaacggtact tggattgtgc aagccggcga atggggcaaa tttgttggac gggctgattt
     1321 tgagtttaaa gatggaaaag ctaccttggt aaaatatgaa ttaattccgg tcaacttaaa
     1381 aaaaaccatt aaaaaagaag acggtactaa agaataccga ctttaccagc ctgaaatcaa
     1441 agccgatgaa gcaacctata ccttattgaa aaaatatcaa gatgagggcg ataaaaagct
     1501 taatattgaa gttggtcgtg tgaaagggtt attagacggc aaacgtgagc atattcgctt
     1561 ccaacaaacc aatctcggtc gtttaattgc acaatcgcaa atggaaaggg taaaagccga
     1621 tatcgggatt atgaactccg gcggtattcg tacctccctt gaagaaggtg taataactta
     1681 taaacatatt ctgactgttc aacctttcgg taatatgatt gcaacggtgg atttaaaagg
     1741 acaagaattg ttagataccc tcaccactat tgctctgaaa gaagttgata ctggcgctta
     1801 tcctcaattt gccggcattt caatggtagt cgaccgtaca gcaaaaacta tttctgatgt
     1861 taaaatcggt ggcaaacctt ttgataaaga gaaaacttat aaagtttctc tgcctgacta
     1921 tgttgcggct ggtggcgatg gctacccatt actgaaaaaa caccctagct atgtcaatac
     1981 aggctttatt gatgccgaaa tgctgaaaaa attcttcgaa gaaaataaaa ttattgaggc
     2041 agataaatac gatccaaaaa accagatcat tttcaaatag agctatagaa aatgaaactc
     2101 actctggcag tctctgctca aaagagctgt ggctgcctgt agattgtttt cttactttca
     2161 agtgttatag gaaatcgata aaaaatccct accaaattaa ttcatcacca tatgatgaaa
     2221 attaaaaaac ctctggcaat actttgccaa aagtctttga cttttatagg gtaatatcac
     2281 tgcttattag cctttaacac gttgttccaa aatcacattc agtgtttttt gtttctgttt
     2341 caacgcattt tcgggttcgg ctttaactag taaggtataa attaaatcaa gaacgaaaag
     2401 ttgtgacatt ttagtaccga tagaatctcc ttgcatatgc ccctgtcgat taccattaat
     2461 taacacataa ttagcctctt ccgtgacggg ggaacgtaaa ttgtgtgtaa tcgcaaccgt
     2521 tgtcgcttta tttgcacgag aaaaacgtag tgatttcaca atttcttcag aatagcctga
     2581 atgactaatc cctataacta aatccccctc tttcaccaat gccgcctgca tatacataaa
     2641 atgattattt gtgactgcat cggtttgtag cccaatacgc attaatttat gttttgcatc
     2701 ttctgcggtt aacccagaag aacccactcc aaataaaaag atccgttttg ccttacgtaa
     2761 ttcttcaaca actttttcca acacgttaaa gtcaagcaaa ttaatagtct cttcaataac
     2821 gttatctaaa ctcactttga gcttctcagc aatatctttc gatgtatccg attctgaaac
     2881 atccgtatca agcaatactc tattatcttg attttgcgta gccaactcta ttgcaagctc
     2941 taatttaaag tcggtataac ctttaaaacc taatgtacga caaaaacgaa taaaagttgc
     3001 ttcaccaaca tctaattgtt tggccacttc agataatgaa cattggctaa gtaattccgg
     3061 ctgagataaa atagcagcag ctattttctt ctctgttttg gttaaactat tttgtagcga
     3121 gctaattgta ttaagaattt ttccccgact agacatagta gcctcctaaa tcaattcaac
     3181 aggtactttc accaccagtt ttttcaatgt tttgttgcca ttcaccgaat ttccaggctt
     3241 atgtggttca taagggagga aaatagcgaa catatttggc ttgaggatca agttattttt
     3301 atcatcaatc tcagctgtta attggtagtc atcttcatct cgatattcat catacttaga
     3361 taaatctggc tggcttaacc cgtattcaat catttcttcg ccgtctatca ataattgaat
     3421 atcgataaat tttcggtgta actctgcttt tttcccctca gctggcgtag tttcaaatga
     3481 cattacattc atgcgaatac cattttctaa ttcttgccag cctaattcca aattaactaa
     3541 atctaaactt tttaattttt tacaaaggcg agcgaatact ggtggaagag attttaaata
     3601 a
//
LOCUS       DANLXS010000219         3581 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.223, whole genome shotgun sequence.
ACCESSION   DANLXS010000219 DANLXS010000000
VERSION     DANLXS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.223"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            1..2055
                     /locus_tag="PXD71_002422"
                     /pseudo
     CDS             1..2055
                     /locus_tag="PXD71_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249230.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            2099..3535
                     /locus_tag="PXD71_002423"
     CDS             2099..3535
                     /locus_tag="PXD71_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3327 domain-containing protein"
                     /protein_id="HDL6292486.1"
                     /translation="MKKTTACILTFLVSSVLYANNITIELKQNDFVQGKLIQKAQQPL
                     SLSIIFPDQSSRLLAQNVFGEQDFMFKSELIGTATFSILANGATVSDQDFTLTIERHI
                     PISAQIAPPKVIENTQLEELSQKIAKNPEKQTALINDFWDKAKQAGTPLIEPLDEQQS
                     RVTFLWRGATKNARIWGGVSTEHDFMQRFGETDIWYKSYIVPNDTLVEYKLAPDVPTL
                     PVDENTQRRALLATAQADPLNKTAYFEKIGQNIHNTDKFNYASLLKLPNAPTQSFLTE
                     APNIAKGKLQQLIFESATLGNKHRLFVYLPDGFSAEKNYAVLYLFDGVEYTERVPTKT
                     ILDNMIAQKVILPTVAVFIENPSSESRRVELPANPIFTQVLAKELVPFVEKQLNIKTK
                     ERIIGGSSYGGLSSAYTVLNYPNVFSKALILSGSFWWQPKGTPSEQSHYIAQQFIQKE
                     KLPLCFFISAGFYESGKSAICKRSNYFA"
BASE COUNT         1180 a          722 c          680 g          999 t
ORIGIN      
        1 ctttctctaa tcactttagc gattgtagct cctcaagtat tagcagaaac aacctctcaa
       61 gcagaactaa atgctattga ggtcaaagag aaattggcag ctaccaccgg ctatattcct
      121 gtttatacga ctgtaggaac gaaaacatta acctctttgg ctcgtacacc ttttagtatc
      181 aatgttgtct ctgatgattt aatgcatgac caagctgttc gttctgttac cgattcagtg
      241 gcttatacct ccggtatgat gggtggctat cgtggtaaca attcgcttat cgaaatctcc
      301 attcgaggca ttggcaataa aagtgatggc agtacaattc cgacctattt aaacggttct
      361 cgctaccaaa ctgcatttga aatcgatccg ttcttcttag aaagtatcaa tgtgattaaa
      421 ggtccaaact ccattttata cgggcaagct aatcccggtg gcgtaatgga tattatcacc
      481 aaaaaagccg gcagtaaccc acatcgccat ttgcaatttg tggtgggaaa ccatcaacga
      541 taccaaatcg ggctggatat tgaagaaaat ctctccaata ccttatccgc tcgtttaatc
      601 ggtcaattag aacgaacaaa ctggaatacc taatttgtaa aagaaaaagg cggtgctttt
      661 gctccaagca ttttatggaa accgactaca aatacagaac ttaatgttta cgcttactat
      721 gcaaaaaaac cgaaagccgg tgatcgtaac tttttaccga aagaaggaac gattaatagc
      781 gtaaacggga aggaactacc ctatgatatg ttcctcagcg atctgaacta tcacggttta
      841 aaagccgaac aaatgcaagt aggttacagt ttctcacata aatttacgcc aaacctaacg
      901 ttccgccaaa atgcaagtta ccataatggc gaagagaatt tcaaaaatct agtttattgg
      961 ggcgatgtcg gttcggtttt agaacgtaga gctcgccatt gggatgtcaa aacccaagaa
     1021 atcaacttag ataaccaatt agaatgggta tttagcacag caaatataaa tcataccttg
     1081 ttagccggta ttgattataa acgagtgcgt aaagatcttg ataactatat ggggattgcc
     1141 cctgattttg attggcagaa ccctgtttat ggcgtgaccg ttactgagcc tagcttattt
     1201 tcaagtgaag tgaagcattt aaaacaactt ggggtttatc tacaagatca aatagaaatc
     1261 ggtaattttg acttcctgtt aggcggtcgt tacgatcgag caaaaaacag caaccaagat
     1321 cgtttgaaca gtaaaacaga gaataaccaa gaaggaaaat ttacttggcg tacaggcgta
     1381 gtttacaact ttgaaaacgg tatcgctccg tatatcagct acagcacttc ttttgtgcca
     1441 tcggctgaaa aagatgctca aggaaatttc ttaaaaccaa caactgctgg gcaatgggaa
     1501 gtgggaatga aataccaacc tacccaagac accttactga ccttagcagg atttaaaatt
     1561 acacaaaaag atttagccaa ttatcaatgg caaacccgct cttatgagca aattggtgaa
     1621 gtgcaaacca aaggaattga agtgagttta aatcagcaat tatccgataa attttcaata
     1681 gcggcttctt atgcttactt aaaaaaagag attactgcag ataaagatgg cacgacgatt
     1741 ggaaaaacgc agtggggagt gcctcgccat caagcatctc tttgggcaaa atataaattc
     1801 tttgataagc tgaccgccgg tgtaggtgta cgctatatgg gaacgacttt tggcgataac
     1861 caaaatactt ttaccgtacc ggcatacaca ctttacgata tgatgctagc ttatgatttt
     1921 atgcctaact taaacttaca gctcaacgca caaaacctga cgaataaaaa atatgtcgca
     1981 agctgtgcca ataatttttc ctgtttctat ggaaaagatc gcaatttaag tgcaacgtta
     2041 aactatcatt tttaatctca acagccctca aatgagggct taaacttttg caaaaaatat
     2101 gaaaaaaacg accgcttgta ttcttacttt tttggtttca tctgtgcttt atgctaacaa
     2161 tataaccatt gagcttaaac agaatgattt tgtgcaaggc aaattgattc agaaggctca
     2221 acagccactt tccttgtcga ttatttttcc tgaccaaagc tctcgcctgc tggctcaaaa
     2281 tgtctttggt gaacaagatt ttatgtttaa atcagaacta atcggcacag ctactttttc
     2341 tattctagcg aatggtgcaa ccgtttcaga ccaagatttt accttaacga ttgagcggca
     2401 tattccaatc tcggcacaaa ttgctccacc taaagtaatt gaaaatacac agcttgaaga
     2461 actcagccaa aaaattgcaa aaaatccaga aaaacagacc gctttaatta acgatttttg
     2521 ggataaagca aaacaagccg gcacaccatt gattgaacca ttggacgaac agcaaagccg
     2581 tgtaactttt ttatggcgtg gagcaacgaa aaatgcccgt atttggggcg gtgtgagtac
     2641 tgaacacgat tttatgcaac gctttggtga aacggatata tggtataaaa gttatatagt
     2701 gcccaatgac actttagtgg aatacaaatt agctccagat gtgccgacat tgcccgttga
     2761 tgagaatacc caacgcagag cgttactggc tacggctcaa gccgatccac tcaataaaac
     2821 agcatacttc gagaaaatcg gacaaaatat tcataatacc gataaattca actacgcttc
     2881 gctacttaaa ttgccaaacg cacctactca atcttttttg actgaagctc caaatattgc
     2941 aaaaggtaaa ctgcagcagt tgatttttga gagtgcaaca ctcggcaata aacacagatt
     3001 attcgtctat ctgcctgacg gttttagtgc agaaaaaaac tatgcggttc tctatttatt
     3061 tgatggtgtt gaatataccg aacgagtgcc aactaaaacc attttagata atatgatcgc
     3121 tcagaaagtg attctgccta ctgtagcggt gtttatcgaa aatccatcga gtgaatcaag
     3181 acgtgtcgag ctgcctgcca atcccatatt tacgcaagta ttggcaaaag aacttgtacc
     3241 ttttgttgaa aaacagctca atataaaaac aaaggagcga attattggcg gctctagcta
     3301 tggcgggcta tcctctgctt acaccgtact caactatcct aatgtattta gtaaagcatt
     3361 gattctatcc ggttcatttt ggtggcaacc caaaggcaca cctagtgaac aaagccacta
     3421 tattgctcaa caatttatcc aaaaagaaaa attaccactc tgttttttca tcagtgcagg
     3481 tttttatgaa agcgggaaat cggctatttg taagcggtcg aattattttg catagaatta
     3541 cactcaattt tgcatagata aaaattaaaa actatgcaaa a
//
LOCUS       DANLXS010000220         3546 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.224, whole genome shotgun sequence.
ACCESSION   DANLXS010000220 DANLXS010000000
VERSION     DANLXS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.224"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(179..769)
                     /locus_tag="PXD71_002424"
     CDS             complement(179..769)
                     /locus_tag="PXD71_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292487.1"
                     /translation="MTTQITTAQINDLLKSHDIQGYANEWQGRRIYINLSAKNNESKG
                     DRNYQLYIDLATGELVSKLGKGLTTRAFDAAVKAFENAFNAKDTAENTAPVESKETEQ
                     ENKQETTKLVITKPTLKGSEKQIDSANEIINLFIAKIGDTSIPVTATQEQITQAQAMV
                     DNFFAMTNAGKWIEKFKGLNDNSEPKQFWLSVKYGW"
     gene            1514..1892
                     /gene="rnpB"
                     /locus_tag="PXD71_002425"
     ncRNA           1514..1892
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PXD71_002425"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1953..2591
                     /locus_tag="PXD71_002426"
     CDS             1953..2591
                     /locus_tag="PXD71_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HDL6292488.1"
                     /translation="MKFIKTIITLIALYSMPVFSHPHSFVDLKTNVIVEGSMLKSFQM
                     EWMLDEIASSELIYEVKNSQDKEKTQQNITAEMVQTALQNHYFSTLYDSQNQPIKFTT
                     KPTATRFEIKNNRVIFYITFHLAKPHELKQSKVRFYTYEPSYYIAMEYNRPADVNTSN
                     ASCKATLVQPQVDSKLRLYASGLDKNQSLDMPENGDYSLGAQFAQKVEIICD"
     gene            2582..>3546
                     /locus_tag="PXD71_002427"
     CDS             2582..>3546
                     /locus_tag="PXD71_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HDL6292489.1"
                     /translation="MRLKATYWGMIVIGFLSLFALYQLYPMILFKVMEWQKNFNLQLS
                     ASLNELAENQSKAGITLVFISFLYGVFHAVGPGHGKFILSSYLSFEKTKLNQAMKITF
                     ASALVQGLVAISLVTVIVVIFTLSRQYFNLTLKWVERGSFGLMILFGIYWCYQVYRTV
                     NRARKPIIKSIRISQKKAGKLPLVTQKGHIHTENCSCRHKHLPTSNEMQQVRDWKAQL
                     MLVLSIGSRPCSGAILVLFLSYTLNLYAWGVISALAMAVGTGLTLSLFAYLVIIARNK
                     AVKVSQWYFSAQINRNIVWILKFTLGLALILFGMVLFHSSFIDAGT"
BASE COUNT         1022 a          588 c          741 g         1195 t
ORIGIN      
        1 ggtatctcaa gattattgtc tgtacgtttg ttggcacatc gtcaccataa gtagcagcga
       61 caatttgccc gataatgcgc ataatttgtg gtgttacttg tgtagtcata ataatctcca
      121 gttatctgtt tgttttagcc gtaagccttt gctcttacgg cttatttgtt atctcaatct
      181 accatccata ttttacggat aaccaaaatt gctttggttc gctattatca tttagacctt
      241 taaatttttc gatccacttt ccagcgtttg tcattgcaaa aaaattatct accattgctt
      301 gagcttgagt aatttgttct tgcgttgctg ttactgggat agaggtatca ccaatcttcg
      361 caataaaaag attgatgatt tcgtttgccg aatcaatttg tttttcagag ccttttaatg
      421 ttggttttgt gattacaagc ttagttgttt cttgtttgtt ctcttgctct gtttctttgc
      481 tttctaccgg cgctgtattt tcagcggtgt ctttagcatt aaaagcgttt tcaaaagctt
      541 ttacggcagc atcaaacgct ctggttgtta atcctttgcc aagtttgctc actaactcgc
      601 ctgtggctag gtcaatgtac aactggtaat tccgatcacc tttgctttcg ttattttttg
      661 ctgaaaggtt aatgtaaatg cggcgacctt gccattcgtt ggcgtagcct tggatatcgt
      721 gagattttaa taagtcgttg atttgtgcgg ttgtaatttg agttgtcatt ttagccccct
      781 ttggctgatg gttattagct ggctccctag ccttgcttta tgcgcctaat tataggcgca
      841 taataatttt attgcaagag tttttttaaa tttttttgca aatttttaaa aaatttattt
      901 taccccctat ccctcaaaga cgatcttgct cttcgagtgc tgaggtattg ctttattgtg
      961 ggtgtttttg tgtgtatttt aaatgtaatt caataattta atttaaaaac aattggttat
     1021 aattaaaatt gattgcggta cacgcccctg aaaatattaa ttcgtgctgt catcaacacg
     1081 atcgtgggcg taaacggaaa agtcagtaga gcggaagcag ataagcggtt aagggattgt
     1141 ttgattgcgc aaaatagacc tatttttgcg tggagcactt ggttggcggt aagatgtttt
     1201 ggatggtatt tttggaatga gaaaaaagga aaatagcttg ttttataatc tgattaaaat
     1261 ttgagtttcg gaaaaatgta tatagtttcg gaaatagaaa ggaaattgat taaataaccc
     1321 tttgaaatta ttgtaataaa atagagaaac caaaattagt tcacttagac ttcaaacgtg
     1381 cgtaattttt acgagtgaat taaagcgaag aggattaagt ttttaaatga acttaatcct
     1441 tttttatttt gccataataa ctacaagcgg ttatttcctt gcaatttttt gcgaaagcat
     1501 tatactctag tgctgagttg gttaggcaat cgctggttta ttgaagcctc taaattcgtg
     1561 aaaacgtaaa ctgggggaca agtaaacgag aggaaagtcc gagctacaca gggcagagtg
     1621 ccggataacg tccggggggc gtgagcctac gaccagtgca acagagagca gaccgccggc
     1681 tttgtcggta agggtgaaag ggtgcggtaa gagcgcaccg tgtgggtggt aacattccac
     1741 agcagggtaa actccactcg tagcaagacc aaataggaac tcaatcggtg gcccgctgag
     1801 agttcgggta ggttgcttga gcgtatgtgt aaatgtacgt ctagaggaat gattgtccac
     1861 gacagaactc ggcttatcga ccaactcact aaattcttga aatgcgaaca gggggaactt
     1921 gttcgctttt ttattttata gtgaaagtaa atatgaaatt tattaagacg attatcacgt
     1981 taattgcatt atattcaatg ccagtttttt ctcacccaca ttcatttgtt gatttgaaaa
     2041 cgaatgttat tgtggaaggc tcaatgttga aatctttcca aatggaatgg atgctagatg
     2101 aaattgcttc ttctgagtta atttatgaag tgaaaaatag ccaagataaa gaaaaaactc
     2161 agcagaatat cactgcagaa atggtgcaaa ccgcattgca aaatcattat tttagcacct
     2221 tatatgatag tcaaaatcag ccgataaaat ttacgacaaa gcccacagcg actcgctttg
     2281 aaatcaagaa taacagagtg attttttata ttacttttca tttagctaaa cctcacgaat
     2341 taaagcagag taaagtgaga ttctatactt atgaaccgag ttattatatt gcgatggaat
     2401 ataatcgccc tgctgatgtc aataccagta atgcaagttg taaagcaaca ttggtgcaac
     2461 ctcaagtaga tagtaagtta cgcttatatg cttccggttt ggataaaaac caatcactgg
     2521 atatgcctga aaatggagat tactctcttg gtgcccaatt tgctcaaaaa gtggagatta
     2581 tatgcgatta aaagcaacat attggggaat gatagtgata ggatttttga gcctatttgc
     2641 tctttatcag ctttatccaa tgatattgtt taaagtgatg gaatggcaaa aaaactttaa
     2701 tttacagctt tccgcttcgc tcaatgaatt agctgaaaat caaagtaaag ccggtattac
     2761 cttagtattt attagttttt tatatggagt atttcacgca gttgggcctg gtcacggaaa
     2821 atttattctg agcagttatc tttcttttga aaaaacaaaa ttaaatcagg cgatgaaaat
     2881 cacttttgcc tcagcattgg tgcagggctt agtggcaatt tcattggtga cggtgattgt
     2941 ggtgattttt actctctctc gccaatattt taatttaacc ctaaaatggg ttgagcgggg
     3001 gagctttggt ttgatgatct tgttcggcat ttactggtgt taccaggtgt atcgaacggt
     3061 gaacagagcg cgaaagccta tcattaaatc cattcgtatt tcgcaaaaaa aagctggaaa
     3121 attaccgctt gtcacgcaga aagggcatat tcataccgaa aattgtagct gtaggcataa
     3181 acatttaccg acttcaaatg aaatgcaaca agtgagagat tggaaagcac agctgatgtt
     3241 ggtattaagt attggctcaa gaccttgctc tggcgctatt cttgttttat ttctttctta
     3301 tacgctcaat ctctatgctt ggggggttat ttcagcatta gctatggcag taggaacagg
     3361 gctgacttta agcttatttg cttatcttgt tatcattgcg agaaataagg cagttaaggt
     3421 tagccaatgg tatttttcag ctcaaattaa tcgaaatatc gtttggatat taaaatttac
     3481 acttggactc gcattaattc tatttggaat ggtattattt cacagtagtt tcattgatgc
     3541 tggtac
//
LOCUS       DANLXS010000221         3538 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.225, whole genome shotgun sequence.
ACCESSION   DANLXS010000221 DANLXS010000000
VERSION     DANLXS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.225"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..291
                     /locus_tag="PXD71_002428"
     CDS             <1..291
                     /locus_tag="PXD71_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292490.1"
                     /translation="TFHLKGKITVTNSSEDVLNLYQLRLLINNEEKERLINQSVSPKN
                     SKSNTNGMTTINFDTVVDLKVGDIVDLQYKTTHELTLLSDSFLSQIYVTQEF"
     gene            292..534
                     /locus_tag="PXD71_002429"
     CDS             292..534
                     /locus_tag="PXD71_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292491.1"
                     /translation="MTKKHYCTGWRNAPANINSCCHQHDRDYGIKGTVSRAEADKRLR
                     HCLISQGNPIKAWVFWVVVRLFGWIYYKEKNINGKS"
     gene            492..695
                     /locus_tag="PXD71_002430"
     CDS             492..695
                     /locus_tag="PXD71_002430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292492.1"
                     /translation="MDLLQGEKYQWKVIKQEISLLSKQASKQASKQASKQASKQASKQ
                     SVAQFKQAPLPFTGQKRMFLKHF"
     gene            complement(878..2473)
                     /locus_tag="PXD71_002431"
     CDS             complement(878..2473)
                     /locus_tag="PXD71_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6292493.1"
                     /translation="MRLSQLSLSVLLLSASSLVAAAPVNTLINCVATPPMKLSPAITN
                     DANDFNASSQQIYNRLLAFKAGELTVEPSLAERWEISDDGLSYTFHLRKDVKFHSNKQ
                     FTPTRTLNADDVVFSFQRQMDLNHPYHKVSDGTYFYYHWMNLPKILKTVEKVDDYTVK
                     ITLQQPNAPFLTTLAMDFLSIYSKEYADKLLAEGKPELLDQAPIGTGPFVFQAYQTDQ
                     AVRYVANPDYFQGKAKFEKLIFSITPDAGTRYAKLKAGECDVIDFPNISDIAQMKQDP
                     KVNLLQRDGLNLAYIGLNSTKPALNNAKVRQALHYATDKKAIVEAVYQGGGTVASNPF
                     PDSVLGYNPNLPQYEFNLEKAKALLTEAGFADGFETEIWVQPVVRPSNPNPRRTAEII
                     QADWAKIGVKAKLVTHEWADFNKRTREGEFSTGTYGWTSRNGDPDNFLFPLLSKSNIP
                     GTNYGRWTDEKFEQLLAQAVQTQNRDERAKLYQQAVEIANEQTPFIPLAHAINYVPLS
                     KRVQGFVQNPFGYTPFYGVSLSE"
     gene            2622..3062
                     /gene="mioC"
                     /locus_tag="PXD71_002432"
     CDS             2622..3062
                     /gene="mioC"
                     /locus_tag="PXD71_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDL6292494.1"
                     /translation="MTSICIITGSTLGGAEYVADHINEVLSEQGFEVELFNQATLADI
                     EGKTNLIIVTSTHGAGELPENIQQLFDDLANSSADFSSMKFGVIGLGSSDYDTFCNAV
                     NIVEEALKAKGASQVCESLRIDVMNNFDHDGSAEEWLPSFVEQL"
     gene            3074..3241
                     /locus_tag="PXD71_002433"
     CDS             3074..3241
                     /locus_tag="PXD71_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDL6292495.1"
                     /translation="MNKISANKAKWIKIAIILIYMFSPVDILPEAILGPFGLVDDAAA
                     LMLLIKTILEK"
     gene            complement(3330..>3538)
                     /locus_tag="PXD71_002434"
     CDS             complement(3330..>3538)
                     /locus_tag="PXD71_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HDL6292496.1"
                     /translation="VIAKLSEFNEVVEAYYTTGNYSIFIKVMTHTIEELHSVLAGKIQ
                     SIDEIQSTETLISLQNPISRDILP"
BASE COUNT         1100 a          698 c          716 g         1024 t
ORIGIN      
        1 acttttcatt taaaaggtaa aataacggtt acaaattcat cggaagatgt gctgaattta
       61 tatcaactca ggctgttaat aaataacgaa gagaaagaaa gactgattaa tcaaagtgtt
      121 tcacctaaaa acagcaaatc aaatacaaat ggtatgacaa caatcaattt tgatactgta
      181 gttgatttaa aagtaggtga cattgttgat ttgcagtata aaacaacgca tgagttgaca
      241 ttactgtcag actcattttt atctcaaatt tatgtgactc aggagtttta agtgactaaa
      301 aaacactact gcacaggctg gcgtaatgcg ccagccaata tcaatagttg ctgtcatcag
      361 catgaccgag actacggtat caagggcaca gtcagccgag ccgaagccga caagcggtta
      421 agacattgct taatttcgca agggaatccg attaaggcgt gggtgttttg ggttgtggtg
      481 aggttgtttg ggtggattta ttacaaggag aaaaatatca atggaaagtc ataaaacagg
      541 aaatatcatt actgagcaag caagcaagca agcaagcaag caagcaagca agcaagcaag
      601 caagcaagca agcaagcaag caaagtgtag ctcaatttaa acaagctccg ctacctttta
      661 caggtcaaaa gcggatgttt ttaaaacact tttaatgatg atttaaaggg cgtttgtttt
      721 cagtttattt tgcataactt ttgtgattac ggctatgcaa aatgatttgc aaaactatgc
      781 aaaataaatc gaccgcttac aattctaaaa gaaaagcagt ctattctcta ccacattttg
      841 caaaaaagtg gcaaaaatag accgcttgta tggcatatca ctccgataaa ctcaccccat
      901 aaaatggcgt gtagccaaac ggattttgca caaaaccttg cacccgtttg ctgagtggta
      961 cataattgat agcgtgagcc agcggaataa atggcgtttg ttcgttggca atttccaccg
     1021 cttgttgata gagcttcgcc cgctcgtcac ggttttgggt ttgtacagct tgagctaata
     1081 actgctcaaa tttctcatcc gtccaacgcc cataatttgt tcccggaata ttagatttgc
     1141 ttaataaagg gaagaggaaa ttatcaggat caccattacg gcttgtccaa ccataagtgc
     1201 cggtagagaa ctcgccttca cgggtgcgtt tgttaaaatc cgcccattcg tgggtcacta
     1261 atttcgcttt caccccgatt ttcgcccaat ccgcctgaat aatttcagcc gttcgacgtg
     1321 ggtttgggtt agaagggcgc actaccggtt gtacccaaat ttccgtttca aatccatcgg
     1381 caaagccggc ttctgttaat agggctttgg ctttttcaag gttaaattca tattgcggta
     1441 aatttggatt atagcccaac accgaatcag ggaacggatt acttgccacc gtgccaccgc
     1501 cttgataaac agcctcgaca atcgctttct tatcggttgc ataatgcaag gcttggcgaa
     1561 ctttggcgtt atttaatgcc ggtttggtgc tgtttaagcc gatataagct aaattcagcc
     1621 cgtctctctg taacaaattc accttcggat cttgtttcat ttgggcaata tcggaaatat
     1681 tcgggaaatc aatcacatcg cattcacccg ctttcagttt ggcataacga gtgcccgcat
     1741 caggggtaat actaaaaatc agtttctcaa attttgcttt accttggaaa taatccgggt
     1801 tcgcaacata acgcaccgct tgatcggttt gataggcttg gaacacaaaa ggccctgtgc
     1861 cgatcggggc ttgatccaat aactccggct tgccttccgc cagcaattta tccgcatatt
     1921 cttttgaata aatcgacaag aaatccatcg ctaaggttgt cagaaaagga gcattaggtt
     1981 gctgtaaggt gattttaaca gtgtaatcgt ccactttttc cactgttttt aaaatcttcg
     2041 gcaaattcat ccaatggtaa tagaaataag taccgtcaga gaccttatgg taagggtggt
     2101 ttaaatccat ctggcgttgg aacgagaaca ctacatcatc cgcattcagc gttcgagttg
     2161 gcgtaaattg tttattggaa tgaaatttca cgtctttacg taaatggaat gtgtaactta
     2221 agccatcgtc actgatttcc caacgttccg ccaaactcgg ctcaaccgtt agttcaccgg
     2281 ctttaaacgc taataagcga ttataaattt gttgtgaact ggcgttgaaa tcattggcat
     2341 cattagtaat tgcaggacta agtttcatcg gtggagttgc cacgcaatta atcaacgtat
     2401 ttaccggagc tgctgccacc aaactgctgg cacttaagag taaaacagaa agagataatt
     2461 gagaaagacg cataaagact ccttaacatt ttctactaac ttactcggca ttaacgtatt
     2521 ttcaaaataa ctaaaggtta taatttataa tcagaataat cagttgcaaa ccaatgattc
     2581 ttggctagaa tattggcagt tttttaaacg agagatttac tatgacatca atttgtatca
     2641 tcaccggcag caccttaggc ggtgcggaat atgttgccga tcacattaat gaggtattaa
     2701 gcgaacaagg ttttgaagtg gaactgttta accaagccac ccttgccgat attgagggca
     2761 aaaccaatct gattatcgtt acctctaccc acggagccgg cgagttaccc gaaaatatcc
     2821 aacagctatt tgacgattta gcaaatagct cggctgattt cagcagtatg aaatttggtg
     2881 taatcggttt aggtagctcc gactatgaca ccttctgcaa tgcagtaaat attgttgaag
     2941 aagctctcaa agcgaaaggg gcaagccaag tttgcgaatc actgagaatt gatgtaatga
     3001 ataatttcga ccacgatggc agtgctgaag aatggttacc atcatttgtt gaacaattat
     3061 aacttaagta tcaatgaaca aaatcagtgc aaataaggca aagtggatta aaatcgctat
     3121 tatcttaatc tatatgttca gccctgtaga tattctaccc gaagcgattt taggcccatt
     3181 cggtttggtt gatgatgccg ccgcattaat gctattgatt aagactattt tggaaaaata
     3241 ggcatagtag acaaaatagt tgcaaaaaac caaaacaaaa cgaccgcttg taacccaata
     3301 caagtggtct tatcttttac ttaattcttt taagggagaa tatctcttga aataggattt
     3361 tgaagtgaaa taagcgtttc tgttgactga atttcatcaa tagactgaat ttttcctgct
     3421 aataccgaat gcaactcttc gatggtatgg gtcattactt taataaaaat agaataatta
     3481 ccagtggtat agtacgcctc aacaacttca ttaaattcac taagtttagc aattactt
//
LOCUS       DANLXS010000222         3475 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.226, whole genome shotgun sequence.
ACCESSION   DANLXS010000222 DANLXS010000000
VERSION     DANLXS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.226"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            19..1821
                     /gene="frdA"
                     /locus_tag="PXD71_002435"
     CDS             19..1821
                     /gene="frdA"
                     /locus_tag="PXD71_002435"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDL6292497.1"
                     /translation="MQSVNFDVAIIGAGGGGLRAAIAAAEANPNLKIALISKVYPMRS
                     HTVAAEGGSAAVIKDTDSFDNHFNDTVGGGDWLCEQDIVEYFVEHSPIEMTQLERWGC
                     PWSRREDGEVNVRRFGGMKIERTWFAADKTGFHILHTLFQTSIKYPNIVRFDEHFVLD
                     ILTDNGEARGCVAMNMMEGTLVQINANAVVIATGGGCRTYRFNTNGGIVTGDGLSMAY
                     RHGVALRDMEFVQYHPTGLPNTGILMTEGCRGEGGILVNKDGYRYLQDYGLGPETPIG
                     KPENKYMELGPRDKVSQAFWQEWRKGNTLKTAKGVDVVHLDLRHLGEKYLLERLPFIC
                     ELAKAYEGVDPAKAPIPVRPVVHYTMGGIEVDMNAETSIKGLFAVGECASSGLHGANR
                     LGSNSLAELVVFGKVAGENAARRALEVTARNQPQIDAQAKDVVARLHALANQEGNESW
                     SDIRNQMGDAMEEGCGIYRTQESMEATVNKIQELKERYKNISVKDKSSVFNTDLLYKI
                     ELGFILDVAQSIACSAVERKESRGAHQRLDYVERDDVNYLKHTQAYYNAEGTPTIKYS
                     DVKITKSQPAKRVYGAEAEAQEKVKKAAEAQAQQ"
     gene            1837..2568
                     /locus_tag="PXD71_002436"
     CDS             1837..2568
                     /locus_tag="PXD71_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HDL6292498.1"
                     /translation="MANLDKMTIEVLRYNPESDSEPHLDRYEVPYDSQTSLLDALGFI
                     KDELEPELSYRWSCRMAICGSCGMMVNGKPKLACKTFLRDYSGFMRIEPLANFPIERD
                     LVVDLSHFIDSIEAIKPYIIDNKAPEGQRTKQTPAQLEKYRQFSMCINCGLCYAACPQ
                     FGLNPEFIGPAAITLAHRYNLDNRDNGREERMKLLSSKNGVWSCTFVGYCSEVCPKHV
                     GPASAVNQGKLESAKDYVISMIKPR"
     gene            2578..2970
                     /gene="frdC"
                     /locus_tag="PXD71_002437"
     CDS             2578..2970
                     /gene="frdC"
                     /locus_tag="PXD71_002437"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDL6292499.1"
                     /translation="MSATKRRPYVRGMKASWWKKLDFYKMYMVREATCLPTVWFCIVL
                     FYGAVALSKGTFTTDFVDFLQNPLVVILNIISLAAMLYHAATLFVMTPAVMSVMVKGK
                     HLPVSVGRNILWAATGVISVIALILVYI"
     gene            2984..3331
                     /gene="frdD"
                     /locus_tag="PXD71_002438"
     CDS             2984..3331
                     /gene="frdD"
                     /locus_tag="PXD71_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDL6292500.1"
                     /translation="MSLDQNPKRSNEPPVWLLFSAGGMVSALFFPVVIFIIGLLLPFG
                     LVSPDNIISFAHTWIGKLAIMVLAIFPMWAGMHRIHHGLHDLKVHTPAGNVIFYGLSI
                     LYTILVIIAVVQI"
BASE COUNT         1018 a          639 c          835 g          983 t
ORIGIN      
        1 taactttcgg agtgcattat gcaaagtgtt aattttgatg tcgcgattat aggtgccggt
       61 ggtggtggct tgcgtgctgc tattgcggcg gctgaagcaa atccaaatct aaaaattgct
      121 ttaatttcaa aagtttaccc aatgcgtagc catacggttg cagcagaagg tggttcagcg
      181 gcggtaatta aagataccga ctctttcgac aatcacttta atgataccgt cggtggtggt
      241 gactggttat gtgagcaaga tattgtggag tattttgtag agcattcgcc gattgaaatg
      301 acacaattag aacgctgggg ttgcccttgg agtcgtcgtg aagacggtga agtgaatgtt
      361 cgccgttttg gaggaatgaa aattgagcgt acttggtttg cggcggataa aaccggtttc
      421 cacattttgc atactttgtt ccaaacttcg attaaatatc cgaatattgt gcgttttgat
      481 gagcattttg tgctggatat tctcaccgac aacggcgaag ctcgtggttg tgtggcaatg
      541 aatatgatgg aaggcacact tgttcaaatc aatgcaaatg cagttgtgat tgcaaccggt
      601 ggcggctgcc gtacttatcg ctttaacact aacggcggta ttgtaaccgg cgacggttta
      661 tcaatggcgt atcgtcacgg tgtggctttg cgtgatatgg agttcgtaca atatcaccca
      721 acaggtttgc caaataccgg tattttgatg acagaaggtt gtcgtggtga aggtggtatt
      781 ttagttaata aagatggcta ccgttacctg caagattatg gtttaggacc ggaaacacct
      841 atcggcaaac cggaaaacaa atatatggaa ttaggccctc gtgataaggt ttcacaagca
      901 ttctggcaag aatggcgtaa agggaacacc ttaaaaaccg caaaaggcgt ggacgtagtt
      961 cacttagatt tacgccattt aggtgaaaaa tatttacttg agcgtttacc gtttatttgt
     1021 gaattagcga aagcctatga aggggtcgat ccggctaaag cgccgattcc tgttcgcccg
     1081 gtggttcact acacgatggg tggtattgaa gtggatatga atgcggaaac ctcaattaaa
     1141 ggcttattcg cagtaggtga atgtgcttca tcaggcttgc acggtgcgaa ccgtttgggt
     1201 tctaactcat tagcagagct tgtggtgttc ggcaaagttg ccggtgaaaa tgcggctcgc
     1261 cgtgcgttag aagtgactgc tcgcaaccag ccacaaattg atgcacaagc aaaagatgtg
     1321 gtggctcgct tgcacgcatt agcgaaccaa gagggtaatg aatcttggtc ggatatccgt
     1381 aaccaaatgg gtgatgcgat ggaagaaggt tgtggtattt accgtaccca agaaagtatg
     1441 gaagcgactg taaacaaaat ccaagagcta aaagagcgtt ataaaaacat cagcgtaaaa
     1501 gataaatcaa gtgtgtttaa cactgattta ctctacaaaa ttgaattagg cttcatttta
     1561 gatgtggctc aatccattgc ttgctctgcg gtagagcgta aagaatctcg tggggctcac
     1621 caacgtttag actatgttga gcgtgatgat gtgaactact taaaacacac acaagcgtac
     1681 tacaatgcag agggtacacc gacgattaaa tacagcgatg tgaaaatcac taaatcacaa
     1741 ccggctaaac gtgtgtatgg tgcagaggct gaagcccaag aaaaagtcaa aaaagcagca
     1801 gaagctcaag cacaacaata gagaaggagt agtaaaatgg caaatttaga taaaatgacc
     1861 atcgaagtgc tacgctacaa ccctgaatca gatagcgagc cacatttaga cagatatgaa
     1921 gtgccgtatg acagccaaac ttcactttta gatgcattgg gcttcattaa agacgaatta
     1981 gaaccggaac tttcttatcg ctggtcttgc cgtatggcga tttgtggttc ttgcggaatg
     2041 atggtaaacg gtaagccgaa gttagcttgt aaaacattcc tgcgtgatta cagcggtttt
     2101 atgcgaattg agccgctggc gaacttcccg attgagcgtg acttagtagt ggatttaagc
     2161 cactttattg atagcatcga ggcaattaaa ccttatatta ttgataataa agcaccggaa
     2221 ggacagcgta ctaaacaaac gccggcacaa ttagagaaat atcgtcaatt ctcaatgtgt
     2281 atcaactgcg gtttatgtta tgcggcttgc ccacaatttg gtttgaaccc tgaatttatc
     2341 ggacctgctg cgattacatt ggctcaccgc tataacttgg ataaccgtga taatggtcgt
     2401 gaagagcgta tgaagctact cagcagcaaa aacggtgttt ggagctgtac tttcgtgggt
     2461 tactgctcgg aagtctgtcc gaaacacgtc ggccctgctt cggctgtaaa ccaaggtaaa
     2521 cttgaaagtg cgaaagatta tgttatctca atgatcaaac cgagatagga ggaagaaatg
     2581 tcagcaacaa aacgtagacc ttatgtaagg ggaatgaaag ccagctggtg gaaaaaactc
     2641 gacttctata aaatgtatat ggtgcgtgaa gctacctgtc ttccaactgt ttggttctgc
     2701 attgtattat tttatggagc agttgcgtta agtaaaggaa catttactac cgattttgtg
     2761 gatttcttac aaaatccgtt agttgttatc ctaaacataa tttcattggc agcaatgtta
     2821 tatcatgcgg caacactttt tgtgatgaca cctgccgtca tgtccgtgat ggttaaaggt
     2881 aaacacttac cagtctcggt tggaagaaat attctttggg cagcaacagg cgtaattagc
     2941 gtaattgcat taatcttggt ttatatttaa gaaggggaag aaaatgagtc ttgatcaaaa
     3001 cccaaaacgt tcaaatgaac cgccggtatg gttgcttttt agtgctgggg gaatggtaag
     3061 tgcattattt ttccctgtgg taatctttat tatcggttta ttactgccat tcgggttagt
     3121 atcaccagat aatatcattt cctttgccca tacttggatc ggcaagctcg ctattatggt
     3181 attagcgatt ttccctatgt gggcaggaat gcaccgtatc catcatggat tacacgacct
     3241 gaaagttcac acgccagcag gtaatgtgat tttctacgga ttgtctatct tatacacaat
     3301 tctagtaatc atcgcagtcg tgcagattta aatgagaaag accatcttgt ataaagcaga
     3361 tggtcttttc atttttaata tgctaacaag cggtcgaatt tataagaaaa tttgcaaata
     3421 ttagaaattt tgccgataaa acgatcgttt tgtattaaat atctgcaaaa aagat
//
LOCUS       DANLXS010000223         3403 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.228, whole genome shotgun sequence.
ACCESSION   DANLXS010000223 DANLXS010000000
VERSION     DANLXS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.228"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            44..2020
                     /gene="cpdB"
                     /locus_tag="PXD71_002439"
     CDS             44..2020
                     /gene="cpdB"
                     /locus_tag="PXD71_002439"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDL6292501.1"
                     /translation="MLNRRKFIQLGATAMLVLGTQPSAFAQTAKNKAGLRVIATTDIH
                     GFLTDFDYYKDAPTEKFGFTRTASLIKQARAEVKNSVLVDNGDLIQGNPISDYQSTKG
                     AKEGKPEPSIMALNAMQYEVGTLGNHEFNYGLSYLDNAIKQAKFPIVNANIVKPGTDE
                     PFFTPYVIQQKEIVDEKGNKHTIKIGYIGFVPPQVMIWDKANLEGKVEARDIVKTAQK
                     YVPIVKKEGADIVVALAHTGPSDEPYKEGAENCAFYLADVKGIDAVIFGHSHRLFPNK
                     EFANSPNADIAKGTMKGVPESMAGYWGNNISVIDLSLAENDGKWGVVDGRAELRPIYN
                     TEEKKATVENHPEIVALLKETHEATRQFVSQPIGKATDNMYSYLALVQDDPTIQIVNQ
                     AQKAYVENVVKGLPELAGLPILSAGAPFKAGGRKNDPTGYTEVDKGELTFRNAADLYL
                     YPNTLVVVKATGAELKEWLECSAGMFKQIDVASDKPQYLLDWTGFRTYNYDVIDGVSY
                     QFDITQPARYDGECKLINPNSHRVVNLTFDGKPVDPKQEFLIATNNYRAYGAKFPGTG
                     DKHIVFAAPDENRQVLANYITTESKAHGQVSPKADNNWRIAPISSNAKLDIRFETSPT
                     DKAKAFIKENAQYPMEYIENDETGFAVYRIDLTK"
     gene            complement(2093..>3403)
                     /locus_tag="PXD71_002440"
     CDS             complement(2093..>3403)
                     /locus_tag="PXD71_002440"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL6292502.1"
                     /translation="SFGFQAFILKYLIHYFNDNFFSRSQHDVVSEYTQFISDTLNIED
                     SGEHIAQLHQLGYLPIRIKAIPEGKSVAVKVPLMTIENTHSSFFWLTNYLETLINVSL
                     WQPITSASIAFAYRKALAEFSEQTCDDNSHLPFQAHDFSMRGMSSLESAESSGAGHLT
                     SFLGTDTIPAISFVTAYYGSQHLIGTSIPASEHSVMSSHGVDELPTFRYLMAKYPNNM
                     LSIVADTTDFWHNITVNLPLLKQEILARPESAKLVIRPDSGDFFTIICGNNSSPVEHE
                     RKGLIECLWDIFGGSINQKGYKVLNPHIGAIYGDGVTYDKMIRILEGLTAKGFASSNI
                     VFGVGAQTYQRNTRDTLGFAIKATSITINGVEKAIFKAPKTDNGLKKSQKGRVKVISY
                     DHYVDELTALDDFSDDLLEVVFENGKLVKSTNFDEIRQNIAQQF"
BASE COUNT         1128 a          649 c          689 g          937 t
ORIGIN      
        1 atattttaca atttaccctt tcatttcatt aaggagatat tttatgttaa atagaagaaa
       61 atttatccag cttggtgcaa ctgcaatgct tgtattgggg acacaaccgt ctgcatttgc
      121 acaaacagcc aaaaataaag ctggcttgcg tgtcatcgcc actacggata ttcacggttt
      181 cttaactgat tttgattatt acaaagatgc tccaacagaa aagtttggtt ttacccgtac
      241 ggcaagccta attaaacaag ctagagcaga agttaaaaac agtgtgttag tcgataatgg
      301 tgacttaatt caaggtaatc ctatttctga ctaccagtca accaaaggtg caaaagaggg
      361 caaacctgaa ccttcgatta tggcattaaa tgcgatgcaa tatgaagtcg gaacgctggg
      421 aaatcatgaa tttaactacg gtttaagcta tttagataat gctattaagc aagcaaaatt
      481 ccctattgtc aatgcaaaca ttgtgaagcc ggggacagat gaaccattct ttacacctta
      541 tgttattcaa caaaaagaaa ttgtagatga aaaagggaat aagcatacca ttaagattgg
      601 ttatatcggt tttgtaccac cacaagtgat gatttgggat aaagctaatc ttgaaggaaa
      661 agttgaagct cgagatattg tgaagaccgc acaaaaatat gtgcctatag taaaaaaaga
      721 aggggcagat attgtcgttg cattggcaca taccggccct tcagacgaac cctataaaga
      781 aggcgctgag aactgtgcgt tttatctagc tgatgtaaaa ggtattgatg cagttatttt
      841 cgggcattca catcgcctgt tcccaaataa agaatttgca aattcaccaa atgccgatat
      901 tgcaaaagga acaatgaaag gcgtaccgga aagtatggct ggctactggg gaaataacat
      961 cagtgtgatt gatttaagct tggccgaaaa cgatggtaaa tggggcgttg tggacggtag
     1021 agcagagttg cgtccaattt ataatacgga agagaaaaaa gcaaccgttg agaaccaccc
     1081 tgaaatcgtt gcattattaa aagaaaccca tgaagcaacc cgtcaatttg tgtctcaacc
     1141 tatcggtaaa gcaactgata atatgtatag ttaccttgcg ttagttcaag atgaccctac
     1201 cattcaaatt gtgaaccaag ctcaaaaagc gtatgtagaa aatgtggtaa aaggcttgcc
     1261 ggagcttgca ggattaccaa ttctaagtgc cggagcaccg tttaaagccg gtggacgtaa
     1321 aaatgacccg acaggctata ccgaagtcga taaaggcgaa ttaactttcc gcaatgcggc
     1381 agacttatac ctttacccaa ataccttagt ggtggttaaa gcaacaggag cagaattaaa
     1441 agagtggtta gagtgcagtg caggaatgtt caaacaaatt gatgtcgctt cggataaacc
     1501 gcaatattta ctggattgga caggtttccg cacctataac tacgatgtaa ttgacggtgt
     1561 cagctatcaa tttgatataa cccaaccggc tcgttatgac ggcgagtgca aattgatcaa
     1621 ccctaattcg caccgtgtgg tgaacttaac ctttgacggc aaaccggttg atcctaaaca
     1681 agaattctta attgccacca acaattaccg tgcttatgga gcaaaattcc cgggaacagg
     1741 cgataaacat attgtgttcg ccgcaccgga cgaaaaccgc caagtgttag cgaattatat
     1801 taccaccgaa agtaaagctc acgggcaggt aagtccgaaa gcggataaca actggcgtat
     1861 tgcaccaatt agctcaaatg caaaattgga tattcgtttt gaaacctctc caacagataa
     1921 agctaaagct ttcattaagg aaaatgcaca atatccgatg gagtatatcg aaaacgatga
     1981 aaccggcttc gcagtttacc gtattgattt aaccaaataa tcagagctaa atacaagcgg
     2041 tctaatttgc aaaaaaatta acaaatttga ccgcttgttt cattccattt ttctaaaatt
     2101 gttgtgctat attttgccga atttcatcaa aattggtcga tttgaccaac ttaccatttt
     2161 caaaaaccac ctctaaaaga tcatctgaaa aatcatccag tgcggttagc tcgtcaacat
     2221 aatgatcata agagattact tttactcgcc ctttttgcga tttttttaag ccgttatcgg
     2281 ttttaggagc tttgaaaatt gccttttcga caccgttaat tgtaattgaa gtcgctttga
     2341 ttgcaaaacc cagcgtatca cgagtattgc gttgataagt ttgagcccca acgccaaaca
     2401 caatattgct ggaagcaaaa ccttttgccg ttaagccctc taaaatccgt atcattttat
     2461 cataagtgac gccgtcacca taaattgccc caatatgcgg attaagcact ttataacctt
     2521 tttggttaat actaccacca aaaatatccc acaaacactc aatcagacct ttgcgttcgt
     2581 gttcaacggg ggaggaattg ttaccgcaaa taattgtaaa aaaatcaccg ctatcagggc
     2641 ggataaccaa ttttgctgat tccggtcgag ctaaaatttc ttgttttaat aatggcaaat
     2701 taacggtgat gttatgccag aaatcggttg tatctgccac aatagatagc atattatttg
     2761 gatattttgc cattaaataa cggaaagtcg gcagctcatc aacaccgtgt gaactcatca
     2821 ccgaatgttc tgatgccgga atagatgtac cgattaaatg ttgagaacca taataggctg
     2881 tcacgaatga aattgcagga atggtatctg tgcctaaaaa tgaggttaga tgtccggcac
     2941 cggagctttc tgctgattct aaggaactca ttccacgcat agagaaatcg tgtgcctgga
     3001 agggtagatg gctattatca tcacaggttt gctctgaaaa ttcggctaga gccttacgat
     3061 aagcaaatgc aatggaagct gatgtaatag gctgccataa tgacacatta atcagggttt
     3121 ccaaataatt agtcagccaa aaaaagcttg agtgagtgtt ttcaattgtc attaaaggaa
     3181 ctttcactgc aacagatttt ccttctggaa tagctttaat tctgataggt aagtaaccga
     3241 gctgatgcaa ttgtgcaata tgttcgccag agtcctcaat attcaatgtg tcagaaataa
     3301 attgagtata ttcagaaaca acgtcatgtt gtgagcgaga aaaaaaatta tcgttaaaat
     3361 aatgaatcaa gtattttaaa ataaatgcct gaaagccaaa tga
//
LOCUS       DANLXS010000224         3376 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.229, whole genome shotgun sequence.
ACCESSION   DANLXS010000224 DANLXS010000000
VERSION     DANLXS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.229"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1425)
                     /locus_tag="PXD71_002441"
     CDS             complement(<1..1425)
                     /locus_tag="PXD71_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL6292503.1"
                     /translation="MTLQIHSIRTKVKTPLAKRIAHALTDRKILQFRFDAHLRNQVWQ
                     RLTKTQKALLNRINAAGIDALPKRELDKLLKELKAEIAKNYQETTAYIQPELSGFFAV
                     EKQALTELYNDEIGYDFFNQVPSYQRKATQQATIIAGMPLESWFEKQGSELSFKFEST
                     IRQGLLEGRQTPEIAKATAELFGISRRHADTLVITAVAKVADDAHKALRDENLDLIKG
                     EMHLSTLDTRTSTVCQVRDGKMWDTDYKPIGHSLPYQRPPLHPRCRSILQLVMKDWKE
                     LGFDDVKEMPKSTRASADGQVSEKTNYEEWLSSKTPEEQDKALGKGKADLWRRGVITF
                     SDMLDQSARPLTLKELYSLKNSSSVDKMDSVELVRKKAVAVEPMITRDVVSIITASGG
                     EPAGLDFRLKSLSSLQRKIDTEIMAGVSKEQAIASIRDVIRYTAILDEQRFVEQYQKM
                     QKDLEKQGYSTIIVKNTWKSNNAYK"
     gene            complement(1373..2776)
                     /locus_tag="PXD71_002442"
     CDS             complement(1373..2776)
                     /locus_tag="PXD71_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HDL6292504.1"
                     /translation="MPNSISADFAALKAKTKIIDDLLGGTQKMRQAGQTYLYKMKMEE
                     EDGYKNRLNRSTLYPALRETLSQMLGRVFFNPINVEDVHKQIEPLFDDIDLEGNNLDV
                     FASRWFYAALAYGVSYCLVDFVRVENARSLAEEKAQNARPYLVHIKPQQVLGFKTAKI
                     NGKTAFTQFRYKETVTEDDGEFASKQVNYIYVYEIGRVRKYKNDEGGLSLVADMQIKA
                     QNTPLAFVPIVPFITKPIDVWGMGEPPLMELAYLNIKHWQSQSDQDNILNIARVPLLA
                     IFSDTQIKSLEIGSSAISLPKDSTMQFIEHSGSSINAGLTSLKELEDQMKTAGAKLLT
                     KTALAMTDSQAKDEAGKEISQLRHYANKFEDALDLAFEYIGHWLGIGVDEVGTSQISG
                     NIDNDLDPNASMATIIQLRNAGVISNHSTFEEAKRRGLLSDSAKWEDEQARLQAEGLS
                     DDFTNPFNSNQSENAAS"
     gene            complement(2789..>3376)
                     /locus_tag="PXD71_002443"
     CDS             complement(2789..>3376)
                     /locus_tag="PXD71_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL6292505.1"
                     /translation="GDYLESSDAQIFRDKYQELEFKPNPDFNGPYYGLDFGFANDPTA
                     CVKCWVFDDDLYIEREAGKVRLELDETAAYLANALPDLSNYTVRADSARPESISYLKR
                     HGLPYIQPVPKWKGSVEDGVEHIKSYRKVYIHPSCTEVLKEFRLYSYKTDRLSGDVLP
                     IIIDAHNHYIDAIRYALNPLIQARGEAKQCALKVY"
BASE COUNT          910 a          765 c          617 g         1084 t
ORIGIN      
        1 cttataagca ttatttgatt tccaagtgtt tttaacaatg atcgtagaat aaccttgttt
       61 ctccaagtct ttttgcattt tttggtattg ctcaacaaaa cgctgttcat ctaagattgc
      121 agtgtagcga ataacatctc gaatacttgc tatcgcttgc tcttttgaca ctccggccat
      181 tatttccgta tcaatttttc gctgcaaaga tgatagtgat ttcaagcgaa aatctaagcc
      241 ggcaggctcg cccccagatg ctgtaataat tgatactaca tcacgagtaa tcataggctc
      301 aactgcaaca gcctttttac ggacaagttc cacactatcc attttatcta ccgaagaact
      361 attttttaat gaatatagct cttttagtgt caatggtcta gcactttgat ctaacatatc
      421 gctaaacgtt atcactcccc gccgccacaa atccgccttg cctttgccta gcgctttatc
      481 ttgctcttcc ggtgttttgc tactgagcca ctcttcatag tttgtcttct ccgacacttg
      541 cccgtctgca ctcgccctag tgcttttcgg catttctttc acatcatcaa agcctaactc
      601 tttccagtct ttcatcacca gttgcagaat cgaacgacag cgagggtgaa gtggtgggcg
      661 ttgatacggc aaactgtgtc cgataggctt gtaatcagtg tcccacatct taccgtctcg
      721 cacttgacaa acagtactgg tgcgggtatc gagcgttgat aagtgcatct cacccttaat
      781 taaatcaaga ttttcatctc gcaatgcctt gtgagcatcg tccgccactt ttgccacagc
      841 agtaatgaca agtgtatcag cgtgccgtct ggatatacca aataattcag ccgtagcttt
      901 ggcaatctca ggcgtttgcc taccctctaa caagccttgc cgaatggtgc tttcaaattt
      961 aaacgaaagc tctgatcctt gcttttcaaa ccaactttca agcggcattc cggcaataat
     1021 agtcgcttgt tgcgtagctt tacgttgata tgacggcact tgattgaaaa aatcatagcc
     1081 gatttcatca ttgtaaagct cagtcagtgc ctgtttttca accgcaaaaa aaccgcttaa
     1141 ttcaggctga atgtaagcgg tcgtttcttg gtaatttttt gcaatttctg ctttgagttc
     1201 tttgagcagt ttatccaact ctcttttcgg taaggcatct atcccggctg cattaattcg
     1261 gtttaataat gccttttgcg tttttgtgag tcgttgccaa acttgatttc gtaaatgagc
     1321 atcaaaacgg aactgcaata ttttacgatc tgttaatgcg tgagcgatcc gtttagctag
     1381 cggcgttttc actttggttc gaattgaatg gatttgtaaa gtcatcgctt aaaccttccg
     1441 cttgtaacct tgcttgttcg tcttcccatt ttgcactatc tgacaataaa cctcgtcttt
     1501 tagcttcttc aaacgtagag tgatttgaaa tgacaccggc attgcgtagc tgaataatgg
     1561 ttgccattga cgcatttgga tctaaatcat tgtcgatatt gcctgaaatt tgcgaagtcc
     1621 caacttcatc aacgccgata cctagccaat gcccaatgta ctcaaaagcc aaatcgagag
     1681 catcttcaaa tttgttagca taatgtctaa gctgtgagat ctctttacct gcttcatctt
     1741 tagcctgact atccgtcatt gctaatgctg ttttagttag cagtttcgca ccggcagttt
     1801 tcatttggtc ttctagttct ttcagactgg ttaaaccggc attaatagac gaacctgaat
     1861 gctcaataaa ctgcattgta ctgtctttcg gcagagatat tgcacttgag ccgatttcaa
     1921 gggattttat ttgagtatca gagaaaatag ctaatagcgg tacacgagca atattcaaaa
     1981 tattgtcttg atctgactga ctttgccagt gcttaatatt taaataagcc agctccatta
     2041 aaggcggctc acccattccc caaacatcaa tcggtttagt gataaatgga acaataggca
     2101 caaatgcaag cggtgtattt tgagctttga tttgcatatc agcaaccagt gataagccac
     2161 cctcatcgtt cttatatttt cggacacgtc cgatttcata aacataaatg tagttcacct
     2221 gtttagaagc aaactcgcca tcatcttctg taactgtctc tttataacga aattgggtaa
     2281 aagcagtttt accgttgatt ttggctgttt taaagcccag cacttgttgc ggcttaatat
     2341 gcaccaaata cggacgtgca ttttgagctt tttcttctgc aagactgcgg gcattctcta
     2401 ccctcacaaa atccaccaag caataagata cgccataagc caatgcagca taaaaccaac
     2461 gagaagcgaa tacatcaaga ttgttaccct ctaaatcaat atcatcaaac aatggttcga
     2521 tttgtttatg cacatcctct acgttgatcg gattaaaaaa cactctcccc aacatctgcg
     2581 aaagcgtttc cctcaatgcc ggataaagcg tagaacgatt taatcggttt ttatatccat
     2641 cttcctcttc cattttcatt ttatagaggt aggtttgacc cgcttgtcgc attttttgtg
     2701 tgccaccgag taaatcatcg atgatttttg tttttgcttt taatgccgca aaatcggctg
     2761 agatggagtt aggcattgtt acctcatttt aataaacctt taatgcacat tgcttcgcct
     2821 cgcctcttgc ctgaataaga ggatttaagg cataacgaat ggcatcaata tagtggttat
     2881 gagcgtcaat aatgataggc agtacatcac cacttaatct gtccgttttg taactataaa
     2941 ggcgaaattc tttcaatacc tctgtacaac ttggatgaat atatacttta cgataagatt
     3001 ttatatgctc aaccccatct tcgactgagc ctttccactt tggaacaggt tgaatataag
     3061 gcaatccatg tcgttttaaa taactgatgg attctgggcg agcagaatct gctctaacag
     3121 tataattact caaatcaggc aaagcatttg ctaaataagc agcggtctca tcgagctcta
     3181 gtcttacttt ccctgcttca cgctcgatat ataagtcatc atcgaacacc caacatttca
     3241 cacaggctgt tgggtcattg gcaaaaccaa agtccagccc gtaataagga ccgttaaaat
     3301 cagggttagg cttaaattct aattcttggt atttatctct aaagatttga gcatcagagg
     3361 attctaagta gtcccc
//
LOCUS       DANLXS010000225         3326 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.230, whole genome shotgun sequence.
ACCESSION   DANLXS010000225 DANLXS010000000
VERSION     DANLXS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.230"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..455)
                     /locus_tag="PXD71_002444"
     CDS             complement(<1..455)
                     /locus_tag="PXD71_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL6292506.1"
                     /translation="MKPINLKNPIHFFAFGFGSGLLKPAPGTWGSLAGLIVAIALWSI
                     TQSALFFGLLTLVSFIAGCVICTKASQDLEVHDDGRIVWDEIVAIFLMFTCLPEYNLL
                     GYLLAFISFRIFDILKPFPIRYFDEKLESGLGIMFDDILAAIFALISLHL"
     gene            complement(460..1440)
                     /gene="thiL"
                     /locus_tag="PXD71_002445"
     CDS             complement(460..1440)
                     /gene="thiL"
                     /locus_tag="PXD71_002445"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDL6292507.1"
                     /translation="MGEFDIIERYFNASQRPPRKDVLLSIGDDCALTSLKPNQQLAIT
                     TDTLVCGTHFLPTISPADLAYKSVASNLSDLAAMGAEPAWISLALTLPEINHDWLAEF
                     SEHFFEILDHYNVDLIGGDTTCGPLSLTITAQGIVPQDRGLFRHNAKVGDWIYVSGTL
                     GDSAGGLQLLLNQQSEQVNWNKEQSYLIQRHLRPTPRVLLGLTIASIANAAIDISDGL
                     LSDLSHILKRSDCGAVLNLENFPLSDVLLSCYSRTEAEYFALNGGEDYELCFTVPNDN
                     KAKLDKALANIGVTYTCIGQIRSAKEGLTLLRHNQPVTLPSQRGYDHFKK"
     gene            complement(1503..1916)
                     /gene="nusB"
                     /locus_tag="PXD71_002446"
     CDS             complement(1503..1916)
                     /gene="nusB"
                     /locus_tag="PXD71_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL6292508.1"
                     /translation="MKISPRRRARECAVQAIYSWYISQNTVEEVELAFVTDQDMKGVD
                     MPYFRKLFRGVVDNVEAIDEALRPYLDRKESDVDPIERSILRLSTYELKFEHDVPFRV
                     VINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK"
     gene            complement(1997..3310)
                     /gene="pilQ"
                     /locus_tag="PXD71_002447"
     CDS             complement(1997..3310)
                     /gene="pilQ"
                     /locus_tag="PXD71_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HDL6292509.1"
                     /translation="MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNM
                     VLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQEDDIVYIHKKEEKAADLTPLPI
                     MNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
                     SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGH
                     YKFGGRLEGNGLNNVTNNLNVNFPITGGASAVLQVSSINSRVLDLELSALEQENSVEI
                     IASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLGLEVVPHLSTQNQIL
                     LDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPI
                     LGSIPFIKRLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKKSK"
BASE COUNT          990 a          659 c          671 g         1006 t
ORIGIN      
        1 agatgaagtg aaatcagggc aaaaatggct gcgagaatat catcaaacat aatgcctaaa
       61 ccgctttcta atttttcatc aaaatagcgg attggaaaag gcttcaaaat atcaaaaatg
      121 cgaaagctga taaaggcgag caggtagcca agaagattgt attcaggcaa acaggtgaac
      181 atcaaaaaga tggcaactat ttcgtcccaa acaattcgcc cgtcatcgtg tacttctaga
      241 tcttggctgg ctttggtgca aataacacaa ccggcaataa aagaaactag ggtgagtaag
      301 ccgaaaaaca gggcggattg tgtgatagac cacaaggcta ttgctacaat tagcccggcg
      361 aggctacccc aggtgccggg agcaggcttt aataagcctg aaccaaagcc aaaggcaaaa
      421 aaatgaatcg gatttttaag attaattggt ttcattgcgt tattttttaa aatgatcgta
      481 gcctcgttgt gagggcaagg tgacaggctg attatgacgt aatagggtta aaccttcttt
      541 agccgaacga atttgcccaa tacatgtata agttactccg atattggcta atgctttgtc
      601 taatttggct ttgttgtcat ttggcacggt aaagcagagt tcgtaatctt cgccaccgtt
      661 taaggcaaaa tattctgcct ctgttcgaga ataacaacta agtaagacgt cagacaaggg
      721 gaaattctca aggtttaata ctgcaccaca gtcactgcgc tttaaaatat ggcttaaatc
      781 agacaataga ccatctgaaa tatcaatcgc tgcattagca atagaggcga tagtcaagcc
      841 aagtaaaact cgaggtgttg ggcgtaaatg gcgttgaatc aggtagcttt gctctttatt
      901 ccaattcact tgctcgcttt gctgatttag cagaagttgc aaaccacctg cactgtcacc
      961 gagtgtgccg gaaacataga tccaatcgcc tactttagca ttatggcgga ataagcctct
     1021 atcttgtggc acaatccctt gagctgtgat tgtcaaagat agtggtccac aagtagtatc
     1081 ccctccaatc aaatcaacat tatagtgatc aagaatctca aagaaatgtt cactaaattc
     1141 agctagccaa tcgtgattaa tttcaggtaa ggttaaggct aaagagatcc atgcaggttc
     1201 tgcacccatt gcggcaagat cgcttaaatt ggacgccaca cttttataag cgagatcggc
     1261 aggtgaaata gtcggtagaa aatgagtgcc gcaaacaagg gtatcggttg tgatcgcaag
     1321 ttgctgattt ggtttgagtg atgtgagcgc acagtcatcg ccgatagaaa gtaaaacatc
     1381 tttgcgaggt gggcgttgag aagcattaaa atagcgttcg ataatatcaa attcacccat
     1441 aaaaaatctc cttgagcgtt ataatcaaaa tccccaattt atgtataaat tggggaatct
     1501 tattattttc tgcctaatgc tggggctaat ttatctaaaa tgccgttaat gtatttgtgg
     1561 ctgtcatcag agccgaacac tttagcgact tcaatccctt cattgatgac tacacggaaa
     1621 ggcacatcgt gttcaaattt taattcgtaa gttgataaac gcaaaataga acgttcaata
     1681 ggatcaacat ctgactcttt acgatctaaa taagggcgta atgcttcatc aatggcttct
     1741 acattatcca caacgccacg gaaaagttta cggaaataag gcatatcaac gcctttcata
     1801 tcttgatcgg tcacaaacgc caattccact tcttcaaccg tgttttggga aatataccaa
     1861 gaataaatgg cttgcaccgc acattctctt gcacggcgac gtggggaaat tttcataaaa
     1921 cctctaaatc tttaactcaa aaaatgacgg ctgattctat cacacttccc ctatttttgc
     1981 ttgcgtttat gatgtgctat tttgattttt tgcggttgct gctcttttca ttagattgaa
     2041 ggatataagg cgtcacaaaa atgactagct ctcttttgct gattttatct cgagtttgac
     2101 taaataaccg cttgatgaag ggaattgagc ctaaaatcgg cactttatct tcgccttttt
     2161 gggttaaatg ttgaaaaata ccaccaagta caatggtttc gccgtgtttg gcaaatactt
     2221 gcgtatttag ctcttgttta tcaatcgtaa ttaagccact actgccggat tgggaatttg
     2281 gcgaattttg ggtaacgatc agatcgagta gaatttgatt ttgggttgaa aggtgtggaa
     2341 cgacttcaag ccctaatacc gcttctttaa attcgacatc ggtggcttcc gatttggtgt
     2401 tgtacatcac ataaggaatt tccgtgcctt gcttgatact tgccggcttt ttattggtgg
     2461 ttaacaggcg tgggctggca ataatttcca cactattttc ttgttctaat gcacttaatt
     2521 ctaaatcaag cactctgcta ttaatggacg aaacctgtaa tacggcagat gctccaccag
     2581 taatcggaaa atttacattt aagttattgg tgacattgtt gagtccattg ccttccagcc
     2641 gtccgccaaa tttatagtgc ccagccccac gagaaaaaat tccccagcga acgccaagct
     2701 cttgcaaatg ctcgctactg atagtgacaa ttctagcctc aatcgcaatt tgctcggtgg
     2761 gttggtcgag ctgttttact aaattctcga tatttttaag cgattttgca ctgtctttca
     2821 caatcaggct attgcttctt tcatcaaagt gaatataacc gttttccgat aaaaacgttc
     2881 cgctgccttt ggttaacgac tcaatcacct ctgaagcctt tgcataatgt agtttcaagg
     2941 tttttgtgat taacttaggg gcggtgacaa gcggtgtatt ttgcccatta ttttgcaaat
     3001 tcattattgg cagaggcgtt aagtcagccg ctttttcctc ttttttgtga atataaacaa
     3061 tatcatcttc ttgcgttaag gataatttat tgactttgcc aagcgtttta aaaatctcgt
     3121 caaccgattt attttctaat cgtagtgttg atttggtttc aatattatca tcaagcacca
     3181 tatttttacc ggtttcttcc gctaaataag taaaaataag cgaagttgga gcatttttta
     3241 aaactatgga aatggtattc gcataggcta atgaaaaacc gcataagaaa tatataaaaa
     3301 ttcgccacat atcaacctct ataatt
//
LOCUS       DANLXS010000226         3317 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.231, whole genome shotgun sequence.
ACCESSION   DANLXS010000226 DANLXS010000000
VERSION     DANLXS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.231"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..633)
                     /locus_tag="PXD71_002448"
     CDS             complement(<1..633)
                     /locus_tag="PXD71_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL6292510.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDVSPAAFGERTLLQPAPTDL
                     ATVKSGKVANFSANSTACKELAVFAKSAKVRSTLNQRDKYSNDLPYIRFVKSIQTCQC
                     RDGKYCHTEKVLVENKNGAVQLCWHHDKMRMDGEIPNEKLEALADENWEKFIAQQIRL
                     TLRKTQSTAIEFADLVLFASIKGLLDELDSKALQQFLGYPPLNNSGVSKES"
     gene            complement(760..1353)
                     /locus_tag="PXD71_002449"
     CDS             complement(760..1353)
                     /locus_tag="PXD71_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL6292511.1"
                     /translation="MTDFDKNTYQTPNYVRNWLNHRYAWFHIDGCSNGQNTLYTYWIG
                     RAADGLDDDKLSCQIADDFLADDLFDVLLDKVSDWGELLRIFVNPPYSDPLPFVQRAA
                     ELKKAGHLVVMLLPADKTTEWYTIIQQHANEVIDIIGYHDEKGTWRTGRIQFINPVTG
                     KPAQGNNKGSMIVVFDPFIEGIVTRQMPLDKIKEWGK"
     gene            complement(1364..2410)
                     /locus_tag="PXD71_002450"
     CDS             complement(1364..2410)
                     /locus_tag="PXD71_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL6292512.1"
                     /translation="MRFTSTINNVRLVEWEINITQGALVDLINQASSWAKAVVIDGIT
                     YYWMSFSKVCEELPAVFSKEDTVYRQYKVLKEKGIIDHFKMDGKDYVRLTEKGCEWNK
                     FEPIRESEKNPSIGNNSEQTRKNFRESSEKNPTDNNTNYKNNNDHTTPLNPPAGEPAP
                     AEVVLNYLNSALVTLAVQLGERKPVGYSLKPWAKNITARIRESSVADCCQVVDYLVAK
                     WGRDEKMREYLCPKTIFRQSNFADYFPKSTAWANNGKPVCVNGKWVTPAELEKRLIMP
                     TVDEVRALFQKSLSGNPFKALDFTNKRNLVMYHATINTRNKRPLERDLPMIISQEIKN
                     AVERIDRLRVPTFS"
     gene            complement(2407..3090)
                     /locus_tag="PXD71_002451"
     CDS             complement(2407..3090)
                     /locus_tag="PXD71_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDL6292513.1"
                     /translation="MNQLLNISAPKASITMSSREIAELCEKEHRHVTRDIEAMFSQLD
                     IPAEGYAHFWTHPQNGQTYREFRLPYDLTLTLISGYSVILRKKIIDRWQELESRQGIP
                     QSFSQALRLAAEQQEVIEQQNHQLAIQAPKVAFVEHYVEVGTTKSLRETAKILNFPEK
                     RLIECLERYRVLYRQSGNLLPYQDKQAKQLFAVKTGTAEHGHNFTQTRVTAKGIEWIA
                     QRYASELGQ"
     gene            complement(3148..>3317)
                     /locus_tag="PXD71_002452"
     CDS             complement(3148..>3317)
                     /locus_tag="PXD71_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL6292514.1"
                     /translation="EHFGTLEKVAATLGISVSAVSQWGEIIPEKNAYRLQEITAGKLK
                     VKHSLYRNRSK"
BASE COUNT          876 a          755 c          632 g         1054 t
ORIGIN      
        1 actttctttg ctcacaccgc tattattcag tggtggatag ccgagaaact gctgtaatgc
       61 cttgctgtct aattcatcaa gcaaaccttt gatactcgca aacaacacta agtccgcaaa
      121 ttcaatagcg gtagattgcg ttttgcgtaa ggttaagcgg atttgttggg cgataaattt
      181 ttcccagttt tcatccgcta gtgcttctaa tttttcattc gggatttcac cgtccattcg
      241 cattttgtcg tgatgccaac aaagctgtac cgcaccgttt ttattctcta ccagcacttt
      301 ttccgtgtgg caatatttgc catctcggca ttggcacgtc tgaattgact ttacaaaccg
      361 gatataaggc aaatcattac tatatttgtc acgctgattt agcgttgaac gaacttttgc
      421 cgatttagca aacaccgcca gctctttaca agcggtcgaa tttgccgaaa aatttgcaac
      481 cttgcctgat ttaaccgtcg ctaaatcggt cggtgcaggt tgcaacaaag ttcgctcacc
      541 gaaagcagca ggagaaacat ctttaggcac tttgtaaaac acaataccca cttcggtttg
      601 aaagtaaggg gtaagtagca aaccttccgc cattattgcc cccatttctc ggtttcatca
      661 taggctttca cgaatagcat aaatacgcca atacagatta aaaaaatcag tacgccaatt
      721 actgcaccaa taccaataaa tagttcccaa taatccattt tatttacccc attccttgat
      781 tttatctagt ggcatctgac gagttacaat gccctcaata aatgggtcaa acacaacaat
      841 catcgagcct ttattattac cttgtgccgg tttacctgtt accgggttga taaattgaat
      901 ccgccctgtc cgccaagtgc ctttttcatc gtgatagcca ataatatcaa ttacctcatt
      961 agcgtgttgt tgaatgatgg tgtaccattc tgttgtttta tccgctggta ataacatcac
     1021 aaccaaatga cctgcttttt tcaattctgc cgctcgttga acaaaaggca gtggatcaga
     1081 atatggcgga ttaacgaaaa tacgcagtaa ttctccccag tctgaaactt tatctagtag
     1141 cacatcaaat aaatcatcgg ctaaaaaatc atcggcaatt tggcaactta atttatcatc
     1201 atctaaaccg tctgccgctc taccaatcca ataggtgtac aacgtatttt gtccgttaga
     1261 gcaaccgtca atatgaaacc aagcataacg atgatttagc caattacgca cataattagg
     1321 tgtttggtaa gtgtttttat caaaatctgt cattgtctaa tccttacgaa aatgttggta
     1381 ctctcaaacg gtcgatacgc tcaaccgcat ttttaatttc ttggctgata atcatcggca
     1441 aatcacgttc aagtggtcgc ttgtttctgg tgttgattgt ggcgtggtac atcactaaat
     1501 ttcgtttgtt ggtaaagtcc aatgccttaa acggattgcc actgagtgat ttctggaaca
     1561 aggctcgtac ttcgtccacc gttggcataa tcaagcgttt ttccagctcc gccggtgtaa
     1621 cccacttacc atttacgcaa acaggctttc cgttattagc ccaagcggtc gattttggaa
     1681 aataatctgc aaaattcgat tggcggaaaa tcgttttcgg gcagaggtat tcacgcattt
     1741 tttcatctcg tccccattta gcgacaaggt aatccaccac ttggcaacag tccgccaccg
     1801 aactttcacg aatgcgagca gtaatatttt ttgcccacgg cttgagcgaa taccccacag
     1861 gcttacgctc accaagctgt accgccaacg ttaccaaagc cgaattgaga taattcaaca
     1921 caacttcagc cggggcaggt tcccccgctg ggggattaag gggggtagta tgatcattat
     1981 tatttttata attagtatta ttatctgtcg gatttttttc cgagctttct cggaaatttt
     2041 tccgagtttg ttcggagtta tttccgatac tcggattttt ttccgactca cggatcggct
     2101 cgaatttatt ccattcacaa cctttttcgg ttaaacggac ataatcttta ccgtccattt
     2161 tgaagtggtc gataatgcct ttttctttca acaccttata ttggcggtaa actgtatctt
     2221 ctttgctaaa aaccgcaggt aactcttcgc aaaccttgct aaatgacatc cagtaatagg
     2281 ttatgccgtc aataaccacc gctttcgccc aactggaggc ttgattgatt aaatcgacca
     2341 atgccccttg tgtaatattg atttcccatt ccactaagcg gacattgttt atggttgatg
     2401 taaatctcat tgtcctaact ccgatgcgta acgttgagca atccattcaa tccctttggc
     2461 ggttactctc gtttgggtaa aattatgacc gtgttctgcc gtgccggttt ttactgcaaa
     2521 aagctgttta gcttgtttat cttgataagg gagtaggttt cctgattggc gataaagaac
     2581 tcgatatcgt tctaaacatt caatcaaccg cttttctgga aaattgagta tcttggcggt
     2641 ttctcgcaat gatttagtcg ttcccacctc aacataatgc tccacaaatg cgactttcgg
     2701 ggcttgaatg gctaattgat gattctgctg ctcaatcact tcttgctgtt ctgccgccaa
     2761 ccgtagagct tgagagaaag attgtggaat accttgtctg ctttctagct cttgccaacg
     2821 gtcgataatt ttcttgcgaa gtatcacgct atagccggaa attagggtaa gggttaaatc
     2881 gtaaggcaat cgaaattccc ggtatgtttg cccattttga gggtgtgtcc aaaaatgggc
     2941 atacccctcg gcaggaatat ctaattgact aaacatcgct tctatatcac gagtaacgtg
     3001 tcgatgttct ttttcgcaca actcagcaat ctcacgacta ctcatcgtaa tgcttgcttt
     3061 tggtgccgaa atgtttaata attgattcat cgaaaatacc tttcgtgttt aattatgtat
     3121 gaaagccacc gttacagcgg tggtttttta tttgcttcta tttctataaa gtgagtgttt
     3181 gacctttaac ttgccggcag tgatctcttg taatctgtac gcatttttct caggaataat
     3241 ctcgccccat tgagaaacag cagatacact aatccctaaa gttgccgcca ctttttctag
     3301 agttccgaaa tgttcga
//
LOCUS       DANLXS010000227         3314 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.232, whole genome shotgun sequence.
ACCESSION   DANLXS010000227 DANLXS010000000
VERSION     DANLXS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.232"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..2240
                     /locus_tag="PXD71_002453"
     CDS             <1..2240
                     /locus_tag="PXD71_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HDL6292515.1"
                     /translation="GRIRSTKMVIYRYVDKSNDTNENLIVEQLNNACSRINGALTTAG
                     LQLQRLDGEQFRQWLLRWFNPNPSINFEGLSNNDCYHLFGKQTSNDGLPELAFNDFSE
                     DLFYSQPKNEGNYWYFDDQPHEVVVVNNLRKQPEVGLMTGEITRGRNINALFDLLPES
                     TIMAITIVIHPQDKLEAHLEKISSRAVGENFESKYLKEDCQTVQSYLKEGHKLYQAAI
                     AFYVRGKDEKELRTRSRQLRTILLSNNLEPVKENAEIAPLNSYLRWLPMNFNPQMDAK
                     SHYYTKYYFVQHLANLLPIFGRDTGTGNPGITYFNRGGAPFNFDPFNRKDRTKNAHKL
                     LLGPTGSGKSATLCGQLCQAMAVYRPRTFVVEAGNSFGLLADYAERFGLTVNRITLAP
                     NSGIVLPLFSEAHRLLDMELNPEDVPEDIEDNDDTDDEGDMQRDLLAEMELTARLMIT
                     GGEKKEDEKMSRADRAAIRRAILNAARQTKSEGRQTLTEDIKNALEALSHDEHTREER
                     RTRLAEMAESLEMFCTGVNGQFFNREGAIWPEADLTLVDLAMFAREGYEAELAISYIS
                     LINHINSLGEKYQHSDRMILNVTDESHIITANPLLAKFLVKGSKMWRKLGIWLWLATQ
                     NMDDFPNEAAKLLSMVEWWELLNVSASEIDEINRFRKLSEAQKTMLLSAKKSDRKYTE
                     GVVLATNMEALFRVVPPSLFLALGMTEKHEKAERKQLMMQHGYTELDAALEVAARIDK
                     ARGIH"
     gene            2240..2668
                     /locus_tag="PXD71_002454"
     CDS             2240..2668
                     /locus_tag="PXD71_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292516.1"
                     /translation="MNKQHYRLFNAGPQKSSLGVVSPAVETQLLGFCEQIRQLIGVGK
                     QITLFPHRVVQNHWAIELSGEQGSVHLLLNVTGRSLLPTSNNSEWSARLIIEIADHVD
                     AYWFSAFLTQYLGLKSNLLPLSTSIADNSVYNEVIDNNEV"
     gene            2658..2975
                     /locus_tag="PXD71_002455"
     CDS             2658..2975
                     /locus_tag="PXD71_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6292517.1"
                     /translation="MKSDTTKPKMTRVITVLKITLMIIGIILLLLVVGWLAMTYFPST
                     NFQAWFYETRYAWLGWRFVLYVAIWLLMVQLNRKGQMPLKARLMILSVIPIIEGLNLL
                     YLL"
     gene            2986..>3314
                     /locus_tag="PXD71_002456"
     CDS             2986..>3314
                     /locus_tag="PXD71_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL6292518.1"
                     /translation="MTFTVDSYLEYFLTLLAWIINNNIFAVLVQTGLFVLPLIFVLIS
                     TWMEVKKQGEDEGNKGDLLITWLSLKFYPAMFVVVLVLAPMIPINLNNIELNVERSKA
                     CGYRVPTA"
BASE COUNT          999 a          618 c          725 g          972 t
ORIGIN      
        1 ctggtcgtat acgtagtacc aaaatggtga tctatcggta tgttgataaa tccaatgaca
       61 ctaatgaaaa tctgattgtc gagcagctga acaatgcttg ttctcgcata aatggagctc
      121 taacaactgc tggattacaa ttgcagcgac tggatggcga acaatttcgt caatggctgt
      181 tacgttggtt taatcccaat ccttctatta attttgaggg gttaagcaac aatgattgct
      241 accatctttt tggtaaacaa acctcaaatg acgggcttcc agaattagcg tttaacgatt
      301 tcagtgaaga tttattttat tctcaaccga aaaatgaagg caactattgg tattttgatg
      361 accaaccgca cgaagttgtt gtagtcaata atttgaggaa acaaccggaa gtggggttga
      421 tgactggcga gattactcgt gggcgtaata tcaatgcatt atttgacttg ttgccggaat
      481 caaccattat ggcaattacc atcgtcatcc atccacagga taagttggag gcacatttag
      541 aaaaaattag tagtcgagcg gtaggtgaaa attttgagtc gaaataccta aaagaagact
      601 gtcaaactgt acagtcttat cttaaagagg ggcataaact ctatcaagca gctattgcat
      661 tttacgttcg aggtaaggat gaaaaggagc tacgtacccg ttctcgccag ttgagaacga
      721 ttctgctcag taacaaccta gaacccgtga aggaaaatgc agaaattgcc ccacttaaca
      781 gctatttgcg ttggctacca atgaatttta atccgcagat ggatgccaaa tcacattact
      841 acacgaagta ctattttgta caacatttgg caaacttatt acctatcttt ggccgagata
      901 ccgggactgg gaatccgggg atcacttact ttaatcgagg aggagcacca tttaatttcg
      961 acccatttaa ccgtaaagac agaaccaaaa atgctcacaa gctattatta ggtccaaccg
     1021 gttcgggaaa atcagctacg ttatgtgggc aattatgtca ggctatggcc gtttatcgcc
     1081 cacggacttt tgttgttgag gcaggaaatt catttggttt attagcggac tatgcagaac
     1141 gtttcggact gactgtaaat cgcattacgt tagctccaaa cagcggcatt gtcttaccac
     1201 tcttttcaga agcacatcgt ctattggata tggaattaaa cccggaagat gtgccggaag
     1261 acattgagga taacgatgac actgatgatg aaggtgatat gcaacgagat ttattggccg
     1321 aaatggagct gacggcacgt ttgatgatca ccggaggtga aaagaaagaa gatgaaaaaa
     1381 tgagccgtgc tgatcgtgct gctattcgtc gtgccatttt aaatgcagct cgccaaacca
     1441 aatctgaagg cagacaaacg ctgactgaag atattaaaaa tgctttggaa gcattaagtc
     1501 atgatgagca tactcgtgaa gaacggcgta cccgtttagc agaaatggca gaatcattag
     1561 aaatgttttg taccggtgta aatgggcaat tttttaatcg agagggggca atttggccgg
     1621 aggctgatct aaccttagtt gatttagcta tgtttgcccg ggaaggctat gaagcagagt
     1681 tggccatttc ctatatttca ctcattaacc atatcaacag tcttggtgaa aaataccaac
     1741 acagtgatcg gatgattctg aatgtaacag atgaatcaca cattatcacg gccaacccat
     1801 tattagctaa attcctagtt aaaggctcaa aaatgtggcg taaactaggg atttggctat
     1861 ggcttgctac tcaaaatatg gatgacttcc cgaatgaagc ggcgaaatta ctctcaatgg
     1921 tcgaatggtg ggaattgttg aatgtgtctg ccagtgaaat tgatgaaatc aatcgtttcc
     1981 ggaaactgtc agaggcccag aaaacgatgt tgttatctgc caaaaaatca gatcgtaaat
     2041 ataccgaggg cgtggtatta gcaacgaata tggaagctct attccgagtt gtaccgccta
     2101 gcttattttt agctttagga atgactgaaa aacatgaaaa agcagaacgt aagcaactga
     2161 tgatgcagca cggctatacc gaactcgatg cagctttaga ggttgcagct cggattgata
     2221 aagcaagagg aatacactaa tgaataaaca acattaccgt ttatttaatg caggcccaca
     2281 aaaatcctca ttaggagttg tatctccagc ggtagaaact caattgcttg gattttgtga
     2341 acaaattcgc caactcatcg gcgttgggaa acagattacc ttattccctc atcgtgtcgt
     2401 gcaaaatcac tgggctatcg aactttctgg tgagcagggt tctgttcact tattacttaa
     2461 tgtgacgggg cgttctttgc taccaacttc caataacagt gaatggtcag ctcgtttaat
     2521 tattgaaata gctgatcatg tggatgctta ttggttttcg gcattcctta ctcagtatct
     2581 cggtttgaaa agtaatcttt tacccttatc cacatcaatt gcggataact ctgtgtataa
     2641 cgaggtaata gataataatg aagtctgata caacaaagcc taaaatgaca agggttatta
     2701 ctgtattaaa aataaccttg atgatcatcg gtattatttt attactactc gttgttggct
     2761 ggttggcaat gacctatttt ccttccacaa attttcaagc gtggttctat gaaacccgct
     2821 atgcctggtt gggatggcga tttgttttat atgtagctat ttggctattg atggtacaac
     2881 ttaatcgaaa agggcaaatg ccattaaaag ccaggttaat gattttatct gtcatcccaa
     2941 ttattgaggg acttaattta ctgtatctac tttaaggagg atgagatgac ctttaccgta
     3001 gatagttact tagaatactt tcttacctta ctggcttgga tcatcaacaa caatattttc
     3061 gctgtattgg ttcaaacagg gttgtttgtt ctgcctctca tttttgtctt aatcagtacg
     3121 tggatggaag tcaaaaaaca aggtgaggac gaaggtaata aaggggattt acttattact
     3181 tggctcagcc tcaaatttta cccggcgatg tttgtcgttg tattggtctt agctccaatg
     3241 ataccgatca acctgaataa tattgagttg aatgtagaac gttctaaagc ctgtggttat
     3301 cgtgttccaa ctgc
//
LOCUS       DANLXS010000228         3309 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.233, whole genome shotgun sequence.
ACCESSION   DANLXS010000228 DANLXS010000000
VERSION     DANLXS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.233"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(31..216)
                     /locus_tag="PXD71_002457"
     CDS             complement(31..216)
                     /locus_tag="PXD71_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HDL6292519.1"
                     /translation="MYFEIYKDAKEEFRWRLKAGNHEVIATNQAYTTKQGCQKGIEAV
                     KKVTPETEVKDLTKAEA"
     gene            complement(332..1654)
                     /gene="pepP"
                     /locus_tag="PXD71_002458"
     CDS             complement(332..1654)
                     /gene="pepP"
                     /locus_tag="PXD71_002458"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDL6292520.1"
                     /translation="MDLAYLAKMPQEEFTARRERVLEQMQDNSALLVFTESEKRRNSD
                     SEYLFRPDSYFWYLTGLAEPKSAVLLVKSEGKTESIIFVRKKDPLMETWNGRRLGIEE
                     APKTLQLNAAFDIDDIETVLAKKLENLTACYCALGIQEWGDNILFATFEKMKVNRQKV
                     PTTLIDWQPMLSEMRLIKSEFEIALIQQACHISSMAHIRAMKQARPNRYEWEIEGEIQ
                     HEFSRFGARFPAYNSIVAGGENACILHYNENDQVLKGGDLLLIDAGAEFAYYAGDITR
                     TFPINGKFSEPQKELYELTLTMQKEAINLLVPNSSIKAANDKAVQILTQGLVRLGILN
                     GDVESLIEQKAYRQFYMHGLGHWLGLDVHDVGDYGTERDRPLQIGMVLTVEPGIYIPK
                     DADVPEQYKGIGIRIEDNLLITEYGNKNLSSGCPKEIADVEQLMQSLN"
     gene            1870..2193
                     /locus_tag="PXD71_002459"
     CDS             1870..2193
                     /locus_tag="PXD71_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6292521.1"
                     /translation="MKLSSELQNQINQLNQLRKQALKQEQDEQKLFEKNKIEDLPHFG
                     TQLNAQRKLLGIELTTLEMQTGISSSTLKRLFKDPSQVKFQTICIVAETLGFNLCAIN
                     THKQE"
     gene            complement(2270..3288)
                     /gene="glpX"
                     /locus_tag="PXD71_002460"
                     /pseudo
     CDS             complement(2270..3288)
                     /gene="glpX"
                     /locus_tag="PXD71_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
BASE COUNT          980 a          772 c          564 g          993 t
ORIGIN      
        1 agccctgaaa taatatcagg gctttataga ttatgcttca gctttggtta aatcttttac
       61 ttctgtttca ggtgtaactt ttttaactgc ttcaatgcct ttttgacaac cttgcttagt
      121 cgtataagct tggttggttg caataacttc gtggttaccc gcttttaaac gccaacgaaa
      181 ttcttctttt gcatctttgt aaatttcaaa atacatataa acactccttc gtgttgtgat
      241 tgcccaaaca ctgtgtttgt gtcgggttag cttaagctaa attccccaaa atatcaaagc
      301 aagcggtcat ttttttacaa aaatttgcga attaatttaa cgattgcatc aactgctcaa
      361 catccgcaat ttctttaggg caaccgctgc ttaagttttt gttgccgtat tcggtaatca
      421 gcaaattatc ttcaatccga atcccaatac ctttatattg ctccggtaca tccgcatctt
      481 taggaatata gataccgggt tcaacagtta acaccatacc aatctgcaaa ggacgatccc
      541 gctccgtgcc gtaatccccc acatcgtgaa catctagccc tagccaatgc cctaatccgt
      601 gcatataaaa ttggcgatag gctttttgct caatcaggct ttccacatca ccgtttaaaa
      661 ttcctaagcg aaccaagccc tgcgtgagaa tctgtacagc cttatcgttt gccgctttaa
      721 tcgagctgtt tggcacaaga agattaatcg cctctttttg catagttaag gttaattcgt
      781 acagctcttt ttgcggttcg gagaatttcc cattaatagg gaaggttcgg gtaatatcgc
      841 cggcgtaata cgcaaattcc gcccctgcat caatcagtaa taaatcgccg cctttaagca
      901 cctgatcatt ttcattgtag tgaagaatac aggcattctc accgccggca acgattgaat
      961 tatatgccgg aaaccttgct ccaaaacggc taaattcgtg ttgaatttcc ccctcaattt
     1021 cccactcata acgattcggg cgagcttgtt tcatcgctcg aatatgtgcc attgaagaaa
     1081 tatgacacgc ctgctgaatt aaggcaattt caaattctga tttaatcaac cgcatttccg
     1141 ataacattgg ctgccaatca atcaaggttg tcggcacttt ttgccgattg actttcattt
     1201 tttcaaatgt tgcaaacaaa atattatcgc cccactcttg tatccctaac gcacaataac
     1261 aagcggtcag attttctaat ttttttgcaa gaacggtttc aatgtcatca atatcaaaag
     1321 ctgcgttaag ctgtaacgtt tttggagctt cctcaatccc taaacgtctg ccattccagg
     1381 tttccattaa agggtctttt ttacgcacaa aaataatact ttccgtttta ccttcgcttt
     1441 ttaccagcaa tacggctgat ttaggttctg ccaaaccggt taaataccaa aagtaactat
     1501 caggtctaaa caagtattca gagtcactat tacggcgttt ttccgattcg gtaaaaacca
     1561 ataacgcaga attatcctgc atttgctcaa gcacacgctc acggcgtgcg gtaaattctt
     1621 cttgtggcat tttagccaaa taagcgagat ccattatttc tcctttttat tatatctata
     1681 tcgaaacctg ccttatttta gcacaaacgc agcataaatt cagcctaacc aaaacagatg
     1741 aaagattata tttgagatct tgattgcaaa aacaaaacag attaagatta aatataacct
     1801 ttacatatct aaacaggtta tatttaacct atgaacacat aaataatcta cttttagctc
     1861 aaaaataaaa tgaaactatc aagcgaacta caaaatcaga taaaccaact aaatcagtta
     1921 cgaaaacagg cactcaaaca agagcaagac gagcagaaat tgtttgaaaa aaataaaatt
     1981 gaagatttgc cacattttgg cacgcagctt aatgctcaga ggaaactatt aggcatagaa
     2041 ttaaccaccc ttgaaatgca aaccggcatt tcaagctcta ccctaaaacg cttatttaaa
     2101 gacccaagcc aagtgaaatt tcagacaatt tgtattgtgg cagaaacact cggctttaat
     2161 ttatgtgcaa ttaatactca caaacaagag taagcgtgac aatgtagtga ttttgattac
     2221 tttgggaaga caaaaaatcg ggctaaaagc ccgatttatc agagaaaagc tataaatcca
     2281 ataaagaaag gagttcgtta tctttacgat cgacataatg gtcggattga attttacgaa
     2341 tggttctaga tttgccacga ataagtaagg tttcagtgct ggcaatattg cctctacgtt
     2401 taatcccttt taacaattca cctgaagtaa tgccggttgc aacaaagacg atattgtcat
     2461 cacgcaccaa atcttctaat ttcaacaccg tattcaccgg tacattcatt tcattacagc
     2521 gtaaaagctc tttttcggta gcagttaaat tttccgggct attccccttc acttggttgc
     2581 gaggaattaa acgtgcttgc atatcgccac ccaatgcacg aaccgcagcc cccgccacta
     2641 ccccttcagg tgctccgccg ataccataaa gcagatccaa ttcgctctct ggcaaacagc
     2701 attgcaccgc agccgcaaca tcgccatcag gaatcgcaat cactcttacc cctaattttt
     2761 gcacatcggc aattattttt tcgtggcgag gcttagcaag atagcaatgg ttaattggga
     2821 aagtaatttg ccctttttcg aggcgacacg gcgaagattt tgttcaagcg gaagatttaa
     2881 atcaatcatt cctttgcatt cctgccccac cactaatttt tccatataca tatcaggggc
     2941 ttttaaaaag gtttctttgc cgcctgctgc taataccgac aacgcatttg cctgccccat
     3001 tgcagtcata cgagtaccgt caatcgggtc aaccgcaata ctgatttcct catcttctag
     3061 gcgggataga ccgattttct cgccaatata aagcatcggt gcttcgtcaa tttcgccctc
     3121 accaattacc acttcgccac ggatttccat ttggttcagc ataaaccgca ttgctttcac
     3181 cgcagcttca tcagcggcat ttttatcccc acggcctagc caagagtaag ctgctagagc
     3241 cgctgcttcc gtaacacgag aaaattcaaa agaaagagtt cgtttcattt ttgttcctta
     3301 gtttcttga
//
LOCUS       DANLXS010000229         3297 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.234, whole genome shotgun sequence.
ACCESSION   DANLXS010000229 DANLXS010000000
VERSION     DANLXS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.234"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(55..627)
                     /gene="pdxT"
                     /locus_tag="PXD71_002461"
     CDS             complement(55..627)
                     /gene="pdxT"
                     /locus_tag="PXD71_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDL6292522.1"
                     /translation="MKIAILALQGAFSEHTDKLQQLGVESMEIRQLADLNQDFDGLIL
                     PGGESTVQGKLLRELGLLEPLRQKILNGLPTLGTCAGLILLAENLVNDDKKHLATLPV
                     TVKRNAYGRQLGSFLVEEEVKYLGKIPLTFIRAPYIESTGKDVEILAKISGKIVGVKY
                     QNQIGISFHPEVNNDLSFHRYFVEMCRKNK"
     gene            complement(627..1499)
                     /gene="pdxS"
                     /locus_tag="PXD71_002462"
     CDS             complement(627..1499)
                     /gene="pdxS"
                     /locus_tag="PXD71_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDL6292523.1"
                     /translation="MTNRYDLNRQLAQMLKGGVIMDVTTPEQACIAEEAGACAVMALE
                     RIPADIRAAGGVSRMSDPKMIKSIQAAVSIPVMAKVRIGHVTEAQILQAIEIDYIDES
                     EVLSPADDTFHINKRKFEVPFVCGAKNLGEALRRINEGAAMIRSKGEPGTGNVVQAVH
                     HLRKMKQEIARLVSHTTDELYHVAKELQVPFDLVQYVHQYQKLPVVTFAAGGVATPAD
                     AALMMQLGAEGVFVGSGIFKSGDPAKRARAIVQAVTNYNDPKLLAELSEDLGEAMVGI
                     NEQEIELLMAARGV"
     gene            1710..3113
                     /locus_tag="PXD71_002463"
     CDS             1710..3113
                     /locus_tag="PXD71_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL6292524.1"
                     /translation="MLTYTFNTQEGTALYEQLYRFLKNDIESNILIAGDKLPSKRAFA
                     KHLGISVMTVETAYQQLVAEGYVNTQPKKGFFVNALNLPEKKEAISVQLQKISSEQYE
                     KNKKWQADLTNRQTLAENFPFSVWTKLVREVLKTHQSELMERAESGGVWTLKFAIANH
                     LRAFRGMIVNPTQIIIGAGTEYLYGLLIQLLGLDKHYALTEPSYDKLRRIYHSYRLST
                     ATVSMPFSIDELNSKNVDVIHTSPSHHFPTGHVMPIAKRYELLAWVAQKAERYIVEDD
                     YDSEFRFVGQPIPALKSIDMLGRVIYMNTFSKTLSSTVRIAYMVLPPELLNRFQQELG
                     FYASTVSNFEQYILAEFIQQGYFEKHINRMRAYYQKKRDKLLKAFKQGNLAEKIAIKE
                     EHSGLHFIVQLNTTLTERQILQAAEEQGIKFVALSAYYQDKSQIQQNAFIIGYSNLKD
                     DQINYVAESLKKILDNE"
BASE COUNT         1029 a          656 c          602 g         1010 t
ORIGIN      
        1 aaactacttt ttctaaaaaa gtataaaaaa taagaggcaa tagccccttt tcttttactt
       61 attttttcgg cacatttcca caaaatagcg gtgaaaactt aaatcattat tcacttcggg
      121 atggaaggat atacctatct gattttggta ttttactcca acaatcttac cgcttatctt
      181 ggctaaaatt tctacatctt ttccagtgct ttctatataa ggcgcacgaa tgaatgtgag
      241 aggaatttta ccgagatatt taacttcttc ttctactaaa aacgagccta attgccttcc
      301 gtaagcatta cgtttcactg ttacaggcaa agtagccaaa tgttttttat cgtcgtttac
      361 caaattttcc gcaagcaaaa ttaaacccgc acaagttcca agcgttggca agccatttaa
      421 aattttttga cgaagcggct caagcaagcc taattcacgt aataatttac cttgaacagt
      481 cgattcgcca ccaggtaaaa tcagaccatc aaaatcttgg tttaaatcag caagttgacg
      541 aatttccata ctttcaaccc ctagttgctg gagtttatca gtatgctcgc taaatgcacc
      601 ttgcagagca agaatagcaa ttttcattag acacctcttg ctgccattag caattcaatc
      661 tcttgctcat taattcctac cattgcttca cctaaatctt cagaaagctc cgcaagtaat
      721 ttaggatcgt tgtagttggt aacagcttgt acaatggccc gcgctcgttt agcaggatcg
      781 cctgacttga aaatacctga gccaacgaag actccttctg cacctaactg catcatcaat
      841 gcagcatcgg caggggttgc aacacctcca gcagcaaagg tgacaacagg caatttttgg
      901 tactgatgta catattgtac taaatcaaat ggtacttgga gctctttagc tacgtgataa
      961 agttcatctg tggtatgtga aaccaagcgg gcaatttctt gtttcatttt gcgaagatga
     1021 tgtactgctt gcaccacatt gcctgttcct ggctcgccct tactacggat catagcagcg
     1081 ccttcattaa tacgacgtag ggcttcgcct aaattttttg ctccacatac aaatggcact
     1141 tcaaatttac gcttgttaat gtggaaggta tcatctgcag gagaaagcac ctcactttcg
     1201 tcaatatagt caatttcgat cgcttgcagg atttgtgctt ctgtaacgtg tccaatacgt
     1261 actttcgcca ttacaggaat ggataccgct gcttggatac tctttatcat cttgggatca
     1321 ctcatacgag aaacgccacc agctgcacga atatctgcag gaattctctc tagtgccatt
     1381 actgcacaag ctccagcttc ttccgcaatg caggcttgct caggggtagt tacatccata
     1441 attacaccgc cttttaacat ttgagcaagt tgtctgttta aatcatagcg attagtcatt
     1501 gtactttttc cttctaattg ttgaataatt gaacttttcg tagtttcgat tagctcggca
     1561 ttaagtttat ttttatattc gctggatgcg agtgttcatc taccaattta aatacactca
     1621 gccaatttga gtgaggaaac agcatgtatt gtaatttaat ctgaactgtt taaaatgacc
     1681 attttatgtt tttcttatca tgtcagatta tgcttaccta cacttttaac acacaagaag
     1741 gcaccgctct ctatgagcaa ctttaccgtt ttctaaaaaa cgacattgaa agcaacatac
     1801 ttatagcagg ggataaactg ccgtctaaac gggcttttgc aaagcattta ggcatttcag
     1861 taatgacagt ggaaacagct taccaacagt tagtagcaga aggttatgtg aatactcagc
     1921 ctaaaaaagg cttttttgtg aatgctttaa atttaccaga aaaaaaggaa gccatatcag
     1981 ttcagctaca aaaaatcagt tcagaacagt atgagaaaaa taaaaaatgg caggcagatt
     2041 taactaatcg acagacttta gcagaaaatt ttccgttttc ggtttggaca aaattagtac
     2101 gcgaagtgct gaaaacacac caaagtgagc taatggagcg tgctgagagt gggggcgtat
     2161 ggacattaaa atttgcgata gccaatcatc ttcgagcctt tagaggaatg atcgttaatc
     2221 caacacagat tattattgga gcgggtacgg agtatcttta cggtttgctc attcagcttc
     2281 taggcttaga taaacattat gcactaactg agcccagtta cgataaattg cgcagaattt
     2341 atcacagtta tcgcttatca acagccacag tatcgatgcc attttcaatc gatgagttaa
     2401 attcaaagaa tgtggacgtg atacatacct cgccatcaca ccattttccg acaggacacg
     2461 taatgccgat tgccaaacgt tatgagttgt tagcttgggt tgcacaaaaa gctgaaagat
     2521 atattgtaga agacgactac gacagcgaat ttcgttttgt aggacaacca attcctgcat
     2581 tgaaatctat tgatatgctc gggcgagtta tctatatgaa tactttttct aaaacgctct
     2641 cttcaactgt acgaattgcc tatatggttt tgccaccaga attactgaat cgttttcagc
     2701 aggaattagg gttttatgct tctaccgttt ccaattttga gcaatatatt ttggcagaat
     2761 ttattcaaca aggctatttt gaaaagcata tcaatcgaat gcgagcgtat tatcaaaaaa
     2821 agagggataa attactaaaa gctttcaagc aaggcaattt agcagaaaag attgcaataa
     2881 aagaagaaca ttcaggcttg catttcattg tacagctaaa cacaacatta acagaaaggc
     2941 aaattttgca ggcagcagaa gaacaaggta taaaatttgt cgcattgtct gcttattatc
     3001 aagataaatc tcaaattcag caaaatgctt ttataattgg gtactctaat ttaaaagatg
     3061 atcagattaa ttatgtagca gagagcctga aaaaaatttt agacaacgaa tagcaagtat
     3121 caaaactgct ttaattttta gcaaattgcc ttaaaaacac gcaagtggta attattttag
     3181 tgatttttgc aaaaaattac caaattctcc ttgcaaggca ttttgttttg cgtataatgc
     3241 atggcacaca acgacgcact gttgtgaacg atttaaaatt gatttccagt gcgtcgt
//
LOCUS       DANLXS010000230         3296 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.235, whole genome shotgun sequence.
ACCESSION   DANLXS010000230 DANLXS010000000
VERSION     DANLXS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.235"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            262..639
                     /locus_tag="PXD71_002464"
     CDS             262..639
                     /locus_tag="PXD71_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL6292525.1"
                     /translation="MDKSQGVVSVNIRGGSGFGRANNAVGPNYMGMLAGKYQFNDSTW
                     IGALFGHSWRKISQNYKIGGGRKVTDSSIDLSKIDDLDKESSTTSPFNAAHLKQKPMS
                     RMAKLEYGDKYQTITLLPKSGSG"
     gene            1369..2145
                     /locus_tag="PXD71_002465"
     CDS             1369..2145
                     /locus_tag="PXD71_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL6292526.1"
                     /translation="MNYSATLSAYIHDLFTPFISYSQTHRAPNIKEIFFSNLGDYGVN
                     TALQPEQAKTTQFGINGFKERVFSEEDKLGFKVLFYNTHLKNYIYNVQHTAVGSAPSL
                     FIFHKNYEKPVNIKGIELEFSYDIGSFYGNLAYARQNTNQPVSFTDASSRVDAASHYG
                     FKTQGFGASKISALPRDYGSLELGTRWFDQRLQIGGVAKYYGKSKRIAIDEFNYTYYP
                     GTHIVKETHRLEETVNKQPIIVDFYVSYEPIKSLILKLEL"
     gene            2749..3243
                     /locus_tag="PXD71_002466"
     CDS             2749..3243
                     /locus_tag="PXD71_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6292527.1"
                     /translation="MSRKYKFHEKSGAYFVSFATVYWVGVFVREIYFQVIVDALDYCR
                     KHKGMILYAYCIMPTHIHLLFQAENQNPSDLIRDFKTFTAKILIQRIKDNLQENRREW
                     LLWMFSRAAERRCNVKQYQFWQHHNQPIEIYSQKFFDEKLNYIHQNPVVSGFVSEPWE
                     WKYN"
BASE COUNT         1122 a          534 c          614 g         1026 t
ORIGIN      
        1 attttttata ggaaagacaa tagaaatgaa gatcaaaaaa tatgtatttc acctgactta
       61 tacaacactt atctgcccct tacctagggt attcgcagag gaaggttata tgttagatac
      121 ggttgaggtt aaagcccaag caataaaaaa acagatgaag ttttcattaa aacaggggca
      181 ataagcaccc gagataaaat cttggaatca aaccaaagct tagagactat tgttcgttct
      241 atgcctggcg catttacaca aatggataaa tctcaaggtg tggtttctgt taatattcga
      301 ggaggttctg gatttgggcg agctaataat gcagtaggcc caaattatat gggaatgctc
      361 gctggcaaat accaattcaa tgactctact tggataggag cattatttgg gcatagttgg
      421 cgcaaaatct ctcaaaatta taaaatagga ggcggacgaa aagttacgga tagctcgatt
      481 gaccttagta aaattgatga tctagataaa gaaagttcta ccacctcacc gttcaatgca
      541 gctcatttaa aacaaaaacc aatgagtcgt atggctaaat tggaatatgg cgataaatac
      601 caaaccatca cactattacc aaagtcaggt agcgggtaga catttaaaaa accaaaacaa
      661 ccagttaaat tatcgttttc acttgcccga gtcaccttgg ttagatgttc aagtggccta
      721 tgcggaaaat caaggcaaac aaaactacaa caaagggagc tgaattaata ataaatattt
      781 aattcaaggt ttggcaatta acaacaaagc gacaacctta aatttgagta acacctttaa
      841 agtgtctccg tttaaagatg gagaatggga agcaatatta ggttttaatc gtttaaagaa
      901 ccgctatagt aaaaatagac atcctgcgga attaaactat aacttagaga atggcgaaac
      961 cagcgaaaac aacgattctg taggaatgac agccgttcgt aaatcgcttt atacttcaac
     1021 tttccagcct gatggcgaac agaagttcga tactttttat ctcgacaatc actttaacta
     1081 tggtatatgg ggattggata taaacgcaaa tattaatacc gctcggttta ttggtcagcg
     1141 ttttaaatat tttccttttg cgattagaga attggagtca gatattagac aggcaagtag
     1201 aaataggcag tttgagcatt taaggtcatt acaatctcaa ttagacaact tgcaaaaaaa
     1261 cattgtacat atattccaga tgaggatttt cctaatgatc ctgaattggg agagtttaac
     1321 tgtcctgatg tcaattttcc tcatacagca acaggaaaac ataaacgaat taattattca
     1381 gcaaccttgt ctgcttatat tcacgattta ttcacgccct ttattagtta ttctcaaaca
     1441 catcgagccc ctaatattaa agaaatattc ttctctaatt taggtgacta tggtgtaaat
     1501 accgcactac aaccagagca agcgaaaaca acacagtttg gtattaatgg attcaaagaa
     1561 cgggtattta gtgaagaaga taaattagga tttaaagtgc tattttataa cactcactta
     1621 aaaaactata tttataatgt acaacatacc gctgttggta gtgctcctag tttattcatt
     1681 ttccacaaaa actatgaaaa accagtaaat atcaaaggga tagaattaga atttagttat
     1741 gatataggct ctttctatgg caacttagct tatgctcgtc aaaatacgaa tcaacccgta
     1801 tcctttacag atgcaagttc aagggtagat gccgcttctc attatggttt taaaactcaa
     1861 ggttttggtg ccagcaaaat ttcagcgtta ccaagagact atggctcatt ggagttaggg
     1921 acaagatggt ttgatcaaag actacaaatt ggcggagtgg caaaatatta cggaaaaagt
     1981 aaacgtattg cgatagatga gtttaactat acttattatc ctggaacaca tattgtgaaa
     2041 gaaactcatc ggcttgaaga aaccgtaaat aaacaaccta ttattgttga tttttacgtg
     2101 agctacgaac caatcaagtc gcttatatta aagttagaac tataaaatgt ctttgacaaa
     2161 aagtacattg atcctcttga tgctaataat gatgcagcaa gtcagagggt ttacaattct
     2221 gatatcagtg atgaacctat ttatacgctc aataattatg caagagggag aactatagtg
     2281 tttagcacaa gttataaatt ctaaatgggg ttgtcctaga taagctagct taaatttcct
     2341 ttaactaatt atttggcata gtgactaaaa ttgagtctgc acagtaggtt aaaaccttat
     2401 aatataagga tttaacccag atggtgcaag cgtccacgct tgtgccagtt aattcctgtt
     2461 ctgaaagtat ttcagtcata agataaaagc ctaaatcagc caagtaaaat ttggtttatt
     2521 tgggcttttg tttttggcac aagcgtggac gcttgcgtca tcattttatt tatccatcca
     2581 ttgctatcat ttcagtattt ctttggctta tcacaagcgg ttaaatttta tcaacacttt
     2641 gcaagtttta atgatttttg cgatctagat cgaaaaagtt atattggctt gtggaattta
     2701 attttgaata gggtattttt cttattcctt tttaagttga gaaaggctat gagccgaaaa
     2761 tataaattcc acgaaaagag cggtgcttat tttgtcagtt ttgcaactgt ttattgggtt
     2821 ggtgtgtttg tgagagagat ttattttcag gtaattgttg atgccttaga ttactgcaga
     2881 aaacataagg gaatgatttt gtatgcctat tgtattatgc caactcatat ccatttatta
     2941 tttcaagcag aaaatcaaaa tccttctgat ttaatacgag atttcaaaac attcaccgct
     3001 aaaatactta ttcagagaat taaagataat ttgcaagaaa accgtcggga atggttgctg
     3061 tggatgtttt ctcgggcggc tgaacgacgt tgtaatgtaa aacaatatca attctggcaa
     3121 catcataacc aacctattga aatctattcg cagaaattct ttgatgaaaa gttaaattat
     3181 attcatcaaa atccagtggt aagtggtttt gtgtctgagc cttgggaatg gaaatataat
     3241 tgaaataaac tgcttgtatt tttgcgatct ctgtcgaaaa attgtgaatt tttact
//
LOCUS       DANLXS010000231         3294 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.236, whole genome shotgun sequence.
ACCESSION   DANLXS010000231 DANLXS010000000
VERSION     DANLXS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.236"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            93..1496
                     /gene="asnS"
                     /locus_tag="PXD71_002467"
     CDS             93..1496
                     /gene="asnS"
                     /locus_tag="PXD71_002467"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL6292528.1"
                     /translation="MSKFPTVSEILSGKIAVGEEATVRGWVRTRRDSKAGLSFLSVYD
                     GSCFDPIQAIINNDLPNYNAEVLHLTAGCSVVVTGKVVESPAEGQAVELQASKVEVLG
                     WVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHCLAQAIHRFFHEQGFYW
                     VATPVITASDTEGAGEMFRVSTLDLENLPRGENGKVDFNQDFFGKESFLTVSGQLNAE
                     TYACALSKVYTFGPTFRAENSNTTRHLAEFWMMEPEVAFADLNDNAKLAEDMLKYVFK
                     AVLAERKDDMEFFVKHVDKEAISRLENFINSPFAQVDYTDAIEILLKSGKEFEFPVSW
                     GIDLSSEHERFLAEEHFKSPVVVKNYPKDIKAFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEVLDQRMNEMGLNPEDYWWYRDLRKYGTVPHSGFGLGFERLIVYVTGLQ
                     NIRDVIPFPRAPRNANF"
     gene            complement(1596..2588)
                     /gene="epmB"
                     /locus_tag="PXD71_002468"
     CDS             complement(1596..2588)
                     /gene="epmB"
                     /locus_tag="PXD71_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDL6292529.1"
                     /translation="MQPIQIKPLWLTELAQAFNNPIDLLQFLELNPNDFEGDIAARKL
                     FALRVPRMFAEKMEKGNPNDPLFLQAMSLQAEFIEAEGFVVDPLEEQQSPAPNILHKY
                     HNRLLFMLKNSCAINCRYCFRRHFPYEEVKSGKAVWQQGLTYIAEHPELEEVILSGGD
                     PLMAKDQDLDWILTQLEQISHIKTLRIHSRLPVVIPNRITTELCERLSKSRLNVVLVT
                     HINHANEIDAVFANKMAQLKKAGVVLLNQSVMLKGVNDNAQTLKRLSDKLFEYGILPY
                     YLHLFDKVAGASHFYIEDEQAGEIYRELQRITSGYLVPKLAREIAKEPNKILKG"
     gene            complement(2642..3211)
                     /locus_tag="PXD71_002469"
     CDS             complement(2642..3211)
                     /locus_tag="PXD71_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292530.1"
                     /translation="MKKLITLSAILAVSVATVAEAKRGGGSFRSVSPSKAVATKPVAS
                     QKSTTNQQTQQKDATFANTPNSATAQPQVRQGSTMANVAMGAAAGYILGDMMSSNAHA
                     NQPSQPADANAQAVNSAQNVANGAVPAIAEFKSIGGQIDPFLVEKTDGYRRYCISGVQ
                     YLVAAQGVQTSPTVMVNPNGTPLQCNLIP"
BASE COUNT          923 a          682 c          692 g          997 t
ORIGIN      
        1 ttccgtaaaa ttattatatt tccccctatt ttcttgtaga atacggaacg tttcttatat
       61 taaaactcac aacaaagaat ttgaggtatt caatgtctaa atttccaaca gtttcggaaa
      121 tcctatccgg caagatagcg gtaggtgaag aagcgacagt gcgtggttgg gtgcgtaccc
      181 gccgtgattc aaaagccggt ttatctttct tgagcgttta cgatggttct tgctttgacc
      241 caattcaagc gattattaat aacgatctac caaattataa tgccgaagta ttacatttaa
      301 ccgcaggttg ctctgtggtg gtgaccggta aagttgtgga atcccctgct gaaggtcaag
      361 ccgttgaatt acaagcaagt aaagtggaag tgcttggttg ggtggaagat cctgatacct
      421 acccaatggc agcgaaacgc cactctatcg aatatctgcg tgaagtcgct cacttacgcc
      481 ctcgtacgaa cttaatcggt gcagttgctc gtgttcgtca ctgtttagct caagctatcc
      541 accgtttctt ccacgagcaa ggtttctatt gggtagcaac acctgtaatt accgcctcag
      601 atactgaagg tgcaggtgaa atgttccgtg tttctacgct ggatttagaa aatctacctc
      661 gtggcgaaaa cggcaaagtg gattttaacc aagatttctt cggtaaagaa tcgttcttaa
      721 ccgtatccgg ccagttaaat gcggaaacct atgcgtgtgc cttaagtaaa gtttatactt
      781 tcggtccgac attccgtgca gaaaactcaa acacaactcg ccatcttgca gaattctgga
      841 tgatggagcc ggaagttgcc tttgccgact taaacgacaa tgcaaaatta gccgaagata
      901 tgctgaaata tgtgtttaaa gcggtgcttg cagaacgtaa agatgatatg gaatttttcg
      961 ttaaacacgt tgataaagaa gcgattagtc gtttagaaaa ctttatcaat tcgccatttg
     1021 ctcaagtaga ttacaccgat gcgattgaga tcttattgaa atccggcaaa gaatttgaat
     1081 tcccggtgtc ttggggaatc gatctttcct ctgaacacga gcgtttctta gccgaagaac
     1141 actttaaatc gccggtggtc gtgaaaaact atccaaaaga cattaaagcc ttctatatgc
     1201 gtttaaatga tgacggcaaa acggttgccg caatggacgt tttagctccg ggaatcggtg
     1261 aaattatcgg tggttctcaa cgtgaagaac gtttagaagt gttagatcag cgtatgaatg
     1321 aaatgggctt aaaccctgaa gattactggt ggtatcgtga tttgcgtaaa tacggtacag
     1381 tgccacactc aggtttcggt ttaggtttcg agcgtttaat cgtgtatgta accgggttac
     1441 aaaacattcg tgatgtaatc ccattcccac gtgctccacg caatgcgaac ttctaatttg
     1501 caaaaaagta agagaattta accgcttgca acaagcggtt aaaaaaggcg aaaattttac
     1561 cattttcgcc ttttgtttct tgatttctgt ttaagctaac ctttcaaaat tttatttggc
     1621 tctttcgcta tttctcttgc cagctttggc acaagatagc ctgaagtgat acgctgtaat
     1681 tctcggtaaa tctcaccagc ctgttcatct tcaatataga aatggctcgc ccctgccact
     1741 ttatcaaata aatgcaaata gtaaggcaaa atcccatatt caaaaagttt atcacttaac
     1801 ctttttaagg tttgagcatt atcgtttaca ccttttaaca tcaccgattg attcagcaat
     1861 acaacgccgg cttttttcag ttgagccatt ttgtttgcaa aaacagcatc aatttcattg
     1921 gcgtgattaa tatgggtaac caacacgaca ttcaagcgag attttgataa ccgttcacat
     1981 aattctgtcg taattcgatt gggaataacg accggcaagc gtgaatgaat acgcagggtt
     2041 ttgatatgcg aaatctgctc taattgcgtt aatatccaat ctaagtcctg atcttttgcc
     2101 atcagcggat caccaccgga cagaatcact tcctccagct cgggatgctc agcaatataa
     2161 gttagtccct gttgccacac agctttaccg cttttcactt cctcataagg gaaatgacgg
     2221 cgaaaacagt aacggcagtt aatcgcacaa ctgtttttta gcataaataa caaacggtta
     2281 tggtatttat gtaaaatatt cggtgctggg ctttgttgct cttccaatgg atctaccaca
     2341 aacccttctg cctcaataaa ttccgcctgt aaagacattg cttgcaaaaa aagcggatcg
     2401 tttgggttac ccttttccat tttttcggcg aacattcttg gcactcgaag agcaaataat
     2461 ttacgggcag ctatatcgcc ctcaaaatca tttggattga gttcaagaaa ttgtaataag
     2521 tcgatagggt tattaaaggc ttgagccagc tcggttagcc aaagcggttt gatttgaatt
     2581 ggttgcattg aatattgtag atacaagggg caggtttttg cctgcccgca aataaaaata
     2641 attatggaat taagttacat tgtaatggcg tgccatttgg attaaccatt acagttggtg
     2701 aggtttgtac accttgtgca gcgactaggt attgcacgcc tgaaatacaa taacggcgat
     2761 aaccatcggt tttttctact aaaaacggat cgatttgacc gccaatactc ttaaattctg
     2821 caatagcagg aactgctcca tttgcaacat tttgtgcaga attgaccgct tgtgcatttg
     2881 catccgcagg ctggctaggc tgatttgcat gtgcatttga tgacatcata tcacctaaaa
     2941 tataaccggc tgctgccccc atcgctacat ttgccatcgt gctgccttga cgcacttgtg
     3001 gctgggcagt tgcactgttt ggcgtattgg caaaagtagc atctttttgt tgcgtttgct
     3061 gatttgtggt tgatttttga gaagctaccg gcttagttgc taccgctttt gacgggctaa
     3121 ctgaacggaa gctcccaccg ccacgttttg cctctgccac ggttgcaaca gaaacggcta
     3181 aaatcgcaga aagagtaatc agttttttca ttcgcattcc ttatcaataa agtttgaaaa
     3241 attatagcaa aaatcgcaag agatctttcg ctaattttgt gaatttaggt tatt
//
LOCUS       DANLXS010000232         3279 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.237, whole genome shotgun sequence.
ACCESSION   DANLXS010000232 DANLXS010000000
VERSION     DANLXS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.237"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..135)
                     /gene="rpmG"
                     /locus_tag="PXD71_002470"
     CDS             complement(<1..135)
                     /gene="rpmG"
                     /locus_tag="PXD71_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL6292531.1"
                     /translation="MAAKGAREKIRLVSTAETGHFYTTDKNKRNMPEKMEIKKFDPVV
                     R"
     gene            complement(147..383)
                     /gene="rpmB"
                     /locus_tag="PXD71_002471"
     CDS             complement(147..383)
                     /gene="rpmB"
                     /locus_tag="PXD71_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL6292532.1"
                     /translation="MSRVCQVTGKRPAVGNNRSHAMNATKRRFLPNLHTHRFWVESEN
                     RFVTLRLTAKGMRIIDKKGIDAVLAEIRARGEKI"
     gene            complement(615..1352)
                     /gene="radC"
                     /locus_tag="PXD71_002472"
     CDS             complement(615..1352)
                     /gene="radC"
                     /locus_tag="PXD71_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL6292533.1"
                     /translation="MEEKMNDFFDEDSQEPTMMPREKLLTQGAASLTDSELLAIFLRT
                     GTKAVPVLALAESVLMAFGSLRQLLNANIDDFCKIYGLGKTKYIQLQASKEMTKRYLA
                     QQMEFSEMIQAPYMAIMYFQTELEEEEREVFMVLFLDNQNRLIYKEKMFFGTINQTAV
                     HPREIIKRALKYNAAAIIVAHNHPSGSCLPSESDRSLTKKIEMACELVDIRFVDHIIV
                     GKGDYFSFEEEKLEMKEHSFLQISDRK"
     gene            1502..2704
                     /gene="coaBC"
                     /locus_tag="PXD71_002473"
     CDS             1502..2704
                     /gene="coaBC"
                     /locus_tag="PXD71_002473"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDL6292534.1"
                     /translation="MLQNKKILVGISGGIAAYKTIEFIRHLKKANAEVRVVLTPAAEA
                     FVTPLTLQAISGNAVSTSLLDPQAELAMGHIELAKWADLIVIAPASADFIARLRMGMG
                     NDLLSTLCLATASPILLAPAMNQQMYKQIAVQENLSVLVNRGVQLIGPNSGFQACGDV
                     GAGRMSEPEEIYQAVLAHFSNNQDLEGLNVTITAGPTREAIDPVRYISNHSSGKMGYA
                     IANAFAERGAKVTLISGPVNLPTPKNVERINVESAVEMEQQAVKFAQKSAIFIGCAAV
                     ADYRMAAVSDQKIKKTDANDELTLKLVKNPDIIANVANLAENRPFVVGFAAETQNVAE
                     YAKSKLERKNLDMICANDVSGGQVFGQDQNALHLFWKNGEKTLPLADKSKLAESLVQE
                     IVKHYREK"
     gene            2704..3021
                     /locus_tag="PXD71_002474"
     CDS             2704..3021
                     /locus_tag="PXD71_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292535.1"
                     /translation="MKLLVSHFHQGEGKFPTFSAGTAVRLGSECVNYPNWFACEIDGW
                     KTFVPSHFISEDKLICDYNPTELVAAQGERVEFLELHYQWAIVKRENEIGWLPCEILR
                     VIG"
     gene            3018..3200
                     /locus_tag="PXD71_002475"
     CDS             3018..3200
                     /locus_tag="PXD71_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL6292536.1"
                     /translation="MKIMQLTSNQYINDIKHILLSARQKAYQAVNSAMVEAYWKIGER
                     IVQEEKNMVKDQNEKV"
BASE COUNT          981 a          683 c          680 g          935 t
ORIGIN      
        1 acgcacaact ggatcaaatt ttttgatttc cattttttca ggcatattac gtttgttttt
       61 atctgttgtg tagaagtgac cagtttctgc tgtagaaact aaacggattt tctcacgagc
      121 gcctttagct gccatttttt agctccttag attttttcgc cacgagcacg gatttcagct
      181 aatactgcat caatgccttt tttatcaata atacgcatac ctttcgcagt taaacgtaag
      241 gttacgaaac ggttttcgct ctcaacccag aaacggtgag tgtgaaggtt tggtaagaaa
      301 cgacgtttag tcgcattcat tgcgtgtgag cggttgttac caactgctgg acgcttgccg
      361 gttacttggc aaactcttga catttgaatt tctccaataa ttttaagctt aaaaatggtt
      421 cgggcgatct cacaaaaggc gttgctcaac caacccctcc gattttgatt gcctcgtcag
      481 gcattaaccc gaccacagtc tatgtaataa agactgccga ttataccgat ttcatttgtt
      541 atattcaagt agaatttata tgcgtatctt ttactgtttc aggagaaatg ttaaaggtta
      601 cataaagcaa gcggtcattt tctatcagaa atttgcaaaa atgaatgctc cttcatctct
      661 aatttctctt cctcgaacga aaaatagtcc cctttgccga caataatatg atccacaaag
      721 cgaatatcaa ccaattcgca cgccatttcg atctttttag ttaaacttct gtccgactca
      781 ctcggcagac aagaacctga ggggtgatta tgagccacaa taatcgccgc ggcattatat
      841 tttaacgctc ttttaataat ttctcggggg tgaacagcag tttgattaat ggtgccgaag
      901 aacatttttt ctttataaat caatcggttt tggttgtcca aaaacagcac cataaaaact
      961 tcccgttctt cttcctccaa ctcggtttgg aaatacataa tcgccatata aggggcttga
     1021 atcatctcgc taaattccat ttgttgagcc agatagcgtt tggtcatctc tttagaggct
     1081 tgtaactgaa tgtatttggt tttgcctaaa ccgtaaattt tgcaaaaatc atcaatattt
     1141 gcattcagta attggcgtaa cgagccaaat gccatcaaca cactctctgc caatgccagc
     1201 acaggcacag ctttcgtacc agtccgtaaa aatatcgcta ataattcgga atcggttaat
     1261 gaggctgccc cctgtgttaa taatttctca cgcggcatca ttgttggctc ttgagaatct
     1321 tcatcaaaaa aatcgttcat tttttcctcc atcaatttag ttaagcataa aagagccaac
     1381 ctttgcagac aaagggattt attcaatttt tcgagcaaga tcgcaaaaat agtgaagttg
     1441 tgagcaactc cttattccgc taaaatatgc aaaataaatc cacgctttaa ggatagtaaa
     1501 gatgttacaa aacaaaaaaa ttttagtcgg catttccggt gggattgcag cctacaaaac
     1561 cattgaattt atccgccact taaaaaaggc aaatgcggaa gtgcgtgtag tgctaacccc
     1621 agcagcagaa gcctttgtaa cgcctttgac cttacaagcg atttccggca atgcggtttc
     1681 cacttcacta ctcgacccac aagccgaact ggcgatgggg catattgagc tggcaaaatg
     1741 ggcggattta atcgttatcg ccccggcaag tgccgatttt attgctcgcc tgcgtatggg
     1801 aatgggcaac gatttacttt ccactctctg ccttgcgact gcaagcccga ttttgcttgc
     1861 accggcaatg aatcaacaaa tgtacaaaca gattgcggtg caagaaaatc tgtctgtttt
     1921 agtcaatcgt ggcgttcagc tcatcggccc gaatagtggc ttccaagcct gtggtgatgt
     1981 gggggctggc agaatgtctg agcctgaaga aatttaccaa gcggtgttgg ctcatttctc
     2041 caacaatcaa gatttagagg gcttaaatgt aaccattacc gccggaccga ctcgtgaggc
     2101 gattgaccct gtgcgttata tcagcaacca cagctccggc aaaatgggct acgcgattgc
     2161 taatgccttt gccgaacgtg gagcaaaggt tacgttgatt agcggtccgg tgaatttacc
     2221 aacgccgaaa aatgtggaac gaattaatgt ggaatctgcg gttgagatgg agcaacaagc
     2281 ggtcaaattt gcccaaaaat ctgcgatttt tattggctgt gcagcggttg ccgattaccg
     2341 aatggcggca gtctctgacc aaaaaattaa gaaaaccgat gccaatgatg aactcacgct
     2401 aaaattggta aaaaaccccg atattatcgc taacgtcgcc aatcttgccg aaaaccgccc
     2461 ttttgtggtt ggttttgccg cagaaacgca aaatgtggcg gagtacgcta aatcgaaact
     2521 cgaacgcaaa aatctggata tgatttgtgc caatgatgtc tcaggcggac aagtgttcgg
     2581 gcaggatcaa aatgcgttgc atttgttctg gaaaaatggt gaaaaaacgc ttcctctggc
     2641 ggataaaagc aaattggcag aaagtttggt acaggaaatt gtaaaacatt atcgagaaaa
     2701 ataatgaaat tactggttag ccattttcat caaggcgaag ggaaatttcc tacattctca
     2761 gctggtactg ccgttcgatt agggagtgaa tgtgtaaact acccaaactg gtttgcctgt
     2821 gaaattgacg gttggaaaac ctttgtgcca agccatttta ttagcgaaga taaattgata
     2881 tgcgattata acccaacgga acttgttgca gcacaaggcg agagagtaga attccttgaa
     2941 ctacattacc aatgggcaat tgtcaaaaga gaaaacgaaa ttggctggct accttgtgaa
     3001 atcttaaggg taataggatg aaaattatgc aacttacgtc aaatcagtat atcaacgaca
     3061 ttaaacatat tcttttatct gctcgccaaa aggcgtatca agccgtgaat tcggcaatgg
     3121 tcgaagccta ttggaaaatc ggtgaacgaa ttgtgcaaga agagaaaaat atggtcaaag
     3181 atcaaaatga aaaagtataa tattgtattt tcagcagata aaaactacct gccatattta
     3241 gagattgcct taaaatcact attagctcat cataatcat
//
LOCUS       DANLXS010000233         3261 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.238, whole genome shotgun sequence.
ACCESSION   DANLXS010000233 DANLXS010000000
VERSION     DANLXS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.238"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..598)
                     /locus_tag="PXD71_002476"
     CDS             complement(<1..598)
                     /locus_tag="PXD71_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6292537.1"
                     /translation="MQERITQQNERLSAYMDGQNVDTAFVETLTNSPELQQKWASYHT
                     IRSVMQGDEIILGRDFSAKMEALLENEEIESQANVEKPKGLLLKLKRWGTPIMQAGIA
                     ASVCLVAVLGVNSMNASDEIAQTQQPVLQTLPFSNSVEAVSYNAPVQDQPTEEQLELQ
                     QRKINALLENHELQRRTNNVKNLTLSEEEKQKAQTSSNT"
     gene            complement(644..1216)
                     /gene="rpoE"
                     /locus_tag="PXD71_002477"
     CDS             complement(644..1216)
                     /gene="rpoE"
                     /locus_tag="PXD71_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDL6292538.1"
                     /translation="MSEQITDQELVEKAQKGDKKAFNLLVVRYQNRVAGLLTRYVARD
                     DIPDIVQESFIKAYRSLESFRGESAFYTWLYRIAVNTAKNHLTSLGRRPPKEDILAED
                     AESYDVGAQLRESDTPENLILSEELKKVVFDTIENLPDELKTAIRLRELEGLSYEEIA
                     EVMECPVGTVRSRIFRAREAIDAKIKPLMQ"
     gene            complement(1438..2982)
                     /gene="leuA"
                     /locus_tag="PXD71_002478"
     CDS             complement(1438..2982)
                     /gene="leuA"
                     /locus_tag="PXD71_002478"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL6292539.1"
                     /translation="MSNNNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVM
                     EVGFPVSSAGDFESVQTIARHIKNSRVAALSRAVAKDIDAAYEALKVAEAFRIHTFIA
                     SSALHVEAKLKRSFDDVVEMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAI
                     NAGATTVNIPDTVGFCLPNEYGNIIHQVMNRVPNIDKAIVSVHCHNDLGMATANSLTA
                     VQNGARQIECTINGIGERAGNTALEEVVMAIKTRQEFMGVDTRINTQEIHRVSQMVSQ
                     LCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEIMSPETIGLKKEKLNLTARSG
                     RAAVKGHMADMGYTETDYDLDKLYEAFLQLADKKGQVFDYDLEALAFIDMQQGDEDRL
                     KLDVITTQLISGLPASAFVQVELDGVRQSATSQGGNGPVEATYNAIMNIVGMELKMSH
                     YNLTAKGEGAEALGQVDIVVEYEGRKFHGVGLATDIVESSARALIHAINAIYRSQKVA
                     DLKAKK"
BASE COUNT          886 a          719 c          581 g         1075 t
ORIGIN      
        1 ctgtattgga agaagtttgt gctttttgct tttcttcctc agataaggtt aaatttttaa
       61 cgttattagt acggcgttgt aactcatggt tttctaataa cgcattaatc ttacgctgtt
      121 gaagctctaa ctgttcttct gttggttgat cttgaactgg cgcattataa cttaccgctt
      181 caactgaatt gctaaatggc aatgtttgta atactggttg ttgcgtttga gctatttcat
      241 cactcgcatt cattgaatta acccctaata ccgcaactaa gcacactgaa gctgcaatac
      301 cggcttgcat aattggagtt ccccaacgct tgagcttgag caataagcct ttaggtttct
      361 ctacatttgc ttgagattca atttcttcat tttccagtaa tgcttccatt ttagcagaga
      421 aatctctccc taaaatgatc tcatcacctt gcatcacact acgaatagtg tgataacttg
      481 cccatttttg ctgtaattca gggctgttag ttaatgtttc tacaaatgct gtatcaacat
      541 tttgcccatc catataagct gaaaggcgtt cattttgttg agtgatgcgt tcttgcataa
      601 ttgactccaa tgatttaaat ctcttctaaa ataatctttc tatctattgc ataagtggct
      661 tgattttagc atcaatcgct tcccttgctc ggaaaatacg ggaacgtaca gtaccaaccg
      721 gacattccat cacttctgcg atttcttcgt aacttagccc ttccaattct cttaaacgaa
      781 tggcggtttt aagctcatca ggtaaatttt caatcgtatc aaacacgact ttctttaatt
      841 cctcggataa tatcaaattc tctggcgtat cactttcacg aagttgcgcg cctacatcat
      901 aactttcagc gtcttcggca agaatatctt cttttggcgg tcttctgcct aaagaggtta
      961 aatgattttt cgcagtattc actgcaatgc gataaagcca cgtataaaaa gcactttcgc
     1021 cacggaaaga ctctaaagaa cgataggctt taataaaaga ttcttgcaca atgtccggaa
     1081 tatcatctct tgcaacataa cgagtcagca aaccggctac tctattttga taacgtacaa
     1141 caagtaaatt aaatgctttt ttatcacctt tttgtgcttt ttcgactaat tcttggtcgg
     1201 tgatctgttc gctcaactgc ggactctcct aaatatcaat atgtcatttt ccattgcaca
     1261 ctttgttagt gtgtgcttta tgacaaaagt tcaaaaaatt tgaaaaattg tgtaattttt
     1321 tacattccta gataaaacct acttaataaa ctaagcaagc ggtcggattt tagcgaaatt
     1381 ttgcaaaaat ttcaaaaaat ccgaccgctt gtctatgtta tgctttcgct ctataagtta
     1441 tttcttcgct tttaaatctg ccactttttg tgaacggtaa atcgcattaa tggcgtgaat
     1501 taaagctcgg gctgaagatt ccacaatatc ggttgccaaa cccacaccgt ggaattttcg
     1561 accttcatat tccactacaa tatccacttg ccctaatgct tctgctcctt cgcctttcgc
     1621 agtcagatta tagtgcgaca tttttagctc cattcccaca atgttcataa tggcattgta
     1681 agttgcctca actggaccat ttcccccttg agatgtagca ctttgacgaa cgccgtctaa
     1741 ttccacctgt acaaaagccg aagccggtaa accgctgatt agttgggtgg taattacatc
     1801 taattttaag cggtcttcat cgccttgctg catatcaatg aaggcaagag cttctaagtc
     1861 ataatcaaac acttgccctt ttttatcagc aagttgcaag aaggcctcat aaagtttatc
     1921 taaatcgtaa tccgtttccg tataacccat atcagccata tgccctttca ccgctgctct
     1981 acctgaacgg gcagttaaat tcagcttctc ttttttcaag ccgatggttt ccggcgacat
     2041 aatttcataa gtatttttat ttttcagcat accgtcttgg tgaatacctg aagagtgagc
     2101 aaaggcattg ctaccgacaa tcgctttgtt cggctgaatc ggcatattgc aaagctggct
     2161 gaccatttgg ctgacacggt gaatttcttg ggtattaatg cgggtatcaa cccccataaa
     2221 ttcttggcgg gttttaattg ccattaccac ttcttcaaga gcggtgttac cggcacgttc
     2281 gccaataccg ttgatagtac actcaatctg tctcgctccg ttttgcaccg ctgtcaatga
     2341 gttggcggta gccataccta aatcgttatg gcagtgaaca gagacaatcg ctttatcgat
     2401 attcggaaca cggttcatca cttggtggat aatattgccg tattcattcg gtaaacagaa
     2461 accgactgta tccggaatat tcactgtggt tgcaccggca ttaatggcag cttctacaat
     2521 gcggcagatg ttatcaattc cagtacgccc agcatcttca caggaaaact caacatcatc
     2581 agtataattt ctggcgcgtt ttaccgctgc aaccgccatt tcgactacat catcaaatga
     2641 acgttttaat ttagcctcaa cgtgtaatgc cgagctggca ataaaggtat ggatacggaa
     2701 tgcttctgct acttttaagg cttcataagc tgcatcaatg tcttttgcaa ccgctcggga
     2761 aagcgccgcc acccggctgt ttttgatatg acgagcgatg gtttgtactg attcaaaatc
     2821 gcccgctgaa gaaactggaa aaccgacttc catcacatca acccctaaac gttctaaagc
     2881 aagggcaatt tgcaattttt cttttacggt taaacttgct tttaatgatt gctcaccatc
     2941 acgtaaagtc gtatcaaaaa taataatgtt attgttgctc atagaagcct ctttttaggt
     3001 gtttttattt gataaaaaaa gcccgcattt taaggtgcgg gcaagaatgt ggatagtcca
     3061 tttacttttt ccaatcaatc tttatcctcg cacaaaatac gataacagag ataatcggag
     3121 taaggtaaat ttttccatgt tgtgttgtat gtttatgttt gcattgttat tcagtagttg
     3181 aggtgtatat tatccttatc caaatcaatg tcaatagcat ttttctcttc acgcaaccgt
     3241 ttaccagccc attaattcaa g
//
LOCUS       DANLXS010000234         3261 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.239, whole genome shotgun sequence.
ACCESSION   DANLXS010000234 DANLXS010000000
VERSION     DANLXS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.239"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            23..1000
                     /gene="pdxA"
                     /locus_tag="PXD71_002479"
     CDS             23..1000
                     /gene="pdxA"
                     /locus_tag="PXD71_002479"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDL6292540.1"
                     /translation="MGDAAGIGPEVIIKALANQRIYELAHPVVIGDRKIIQRALPIVH
                     SELKLRILNNLDNFVAEYGVIDILDLNNLPEDLPFAQVDPRAGKAAYEYIEEAVNLAM
                     GGKIHAIVTAPLNKEALHAGGKPFPGHTEILATLSNTKDYSMMLTSEKLNVIHVTTHV
                     QLRKACDLITKERVATVIDLAEENARILGFKEPRIAVAGLNPHSGENGMFGDEDQKEI
                     VPAIQEAQAKGFNVTGPVPPDTLFHRAANLNEFDIVVVMYHDQGHIPIKLLGFDTGVN
                     VTVGLPFIRTSVDHGTAFPIAGKGTADSRSMTEALYLGAKMANIKYATH"
     gene            1011..2009
                     /locus_tag="PXD71_002480"
     CDS             1011..2009
                     /locus_tag="PXD71_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDL6292541.1"
                     /translation="MSTQTSKSIVPIYDGMNKIPGGAMVIPLILGSIIGTFFPEFLNL
                     GSFTTALFKSSAGPLIALLIFATGMQITVRSSGPVLAHTGVVLLFKTIIPALLVVILG
                     LWVGNDGVMGVSLLAMLTVVANSNGGIWLAFTGKYGDYRDKGAYVASAVNDGPFFVLL
                     FLGVSGLADIPLSLMLAAVIPLIIGMVIGNLDPKWTELMKPTGAIVIPFFAFALGTGI
                     NLQSIITGGATGIILGIVASLFTGFLVFIGYKTILRRGNQSGIGFAAGTTAGNAIITP
                     EIVAQADPTLAPFVQTATAQIAAAVLVSAILAPLFAAWVLKRQGGLEQDTIKGENL"
     gene            2006..3232
                     /locus_tag="PXD71_002481"
     CDS             2006..3232
                     /locus_tag="PXD71_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL6292542.1"
                     /translation="MKETLLVIADDLTGANDTAVMFAESGFDTVLKTKVSALAQIHPD
                     KAQVISVSTDSRAIGEKAKELTQIAISNAIQNSIGQIYLKIDSTMRGSVKYQIEGAIK
                     AWAGLYPDVKAIICPAYPEMGRTIEAGHLYVNGVPVNETASGKDPICPVHSSSMQKLL
                     PDAQVIPCDTVDNLQASIENSEYKQIVIDAKTHEDLSMIAQVINHIGNKIIPVGSAGL
                     AQKLKNIQNQQQPERNLKLGRALIVVTSIHETSQLQVDEYISTLGGKSIVFNPSPSQL
                     INYSQSEHSLKLQLNALIRFSRDHVIIRANPAKVVNAKSANITETSKEISQHLADLSK
                     FCLDNEKFDSLILFGGDGAAALLDQMNVTEMNVQYAVVPGVPLCSIEKGNYSGITVMT
                     KSGGFGNNTLLSNIMN"
BASE COUNT         1032 a          586 c          631 g         1012 t
ORIGIN      
        1 gaaacctata ttgggaatta cgatgggaga tgccgccggt atcggaccag aagttattat
       61 taaagcatta gctaatcaac ggatttatga gttagcacac cctgttgtga tcggtgatcg
      121 aaaaattata caacgagcct tacctattgt tcattcagaa ttgaaattaa gaattctcaa
      181 taatctagat aactttgtag ccgaatatgg cgttattgat attttagatt tgaataacct
      241 gcctgaagat ttgccttttg ctcaagttga tccgcgtgct ggaaaagcag cttatgaata
      301 tattgaagag gcagttaatt tagcaatggg aggaaaaatc cacgctattg ttacagcacc
      361 actgaataaa gaagccctac acgcaggtgg taaaccattt cctggccata cagagatcct
      421 tgcaaccctc agtaatacta aagattactc aatgatgcta accagtgaaa aactcaatgt
      481 tattcacgtt actactcacg tccaacttcg taaagcctgt gatttaatta ccaaagaacg
      541 agttgctaca gttattgatt tagcagaaga aaatgctcgc attcttgggt ttaaagaacc
      601 tcgtattgca gtcgctggtt taaatcctca ttcaggtgaa aatggaatgt ttggcgatga
      661 agatcaaaaa gaaattgttc ccgcaattca agaggctcaa gccaaagggt ttaatgttac
      721 agggcctgtc cctccggata ctttatttca ccgtgcagct aacctaaatg aattcgatat
      781 tgttgttgtg atgtatcacg atcaagggca tatccctatc aaattattag gctttgatac
      841 aggtgtaaac gttactgtcg gtcttccatt tattcgaaca tctgtcgatc acggaactgc
      901 tttcccaata gcaggcaaag gaacagcaga tagtcgtagt atgactgaag cactctattt
      961 gggtgcaaaa atggcaaata tcaaatatgc gacacactaa ggagattaat atgtctactc
     1021 aaacatctaa atccatcgtc ccgatatatg atggtatgaa taaaattcct ggcggtgcaa
     1081 tggtaatacc gctaattctt ggttctatta ttggtacatt ctttcctgaa tttttaaatt
     1141 taggttcatt tacgacggct ttattcaaat catcagcagg accacttatt gcactattaa
     1201 ttttcgctac aggtatgcaa attaccgtga gaagttctgg acctgtactt gcccataccg
     1261 gtgttgtatt actatttaaa actattattc ccgctctttt agttgtgatt ttaggattat
     1321 gggtaggtaa tgatggtgtt atgggcgttt ctttattagc tatgctaact gttgttgcaa
     1381 attcaaatgg tggtatttgg ttagcgttta cagggaaata tggtgattat agagataaag
     1441 gagcttatgt cgcttcggct gttaatgatg gacctttctt tgttttacta tttttaggtg
     1501 tatcaggatt agcagatatt cctctttcat taatgcttgc agctgttatt cctcttatta
     1561 ttggtatggt tattggtaac ttagatccta aatggacaga gttaatgaaa ccaacaggtg
     1621 caatagtcat tcctttcttt gcttttgcat taggaacagg aatcaatctg caatctatta
     1681 ttactggagg agcaacagga attattttag gtatcgtagc ttctctcttt acaggttttc
     1741 ttgtatttat cggttacaaa acaattttaa gacgtggtaa tcaatcagga atcgggtttg
     1801 cagcaggaac tactgctgga aatgccatta ttacacctga aattgttgct caagcagacc
     1861 ctacattagc accttttgtt caaactgcga ctgcacagat tgccgcagct gtattagttt
     1921 cagcaatctt agctccatta tttgcagcgt gggttttaaa acgtcaaggt ggcttagaac
     1981 aagacactat aaaaggagaa aatctatgaa agagacactc ttagttattg ctgatgacct
     2041 tactggtgca aatgataccg cggttatgtt tgctgaatca ggatttgaca ctgtcttaaa
     2101 aactaaagtt tcggcattag cccaaattca tcctgataaa gcccaagtaa tttctgtttc
     2161 tacagattca agagcaattg gagagaaagc aaaagagctg actcaaattg caattagcaa
     2221 tgctattcaa aatagtatcg ggcaaattta ccttaaaatt gattccacaa tgagaggctc
     2281 tgttaaatat cagattgaag gtgctattaa agcttgggct ggtttatatc ccgatgttaa
     2341 agcaattatt tgtcctgcat atccagaaat gggaagaact atagaagctg gtcatttata
     2401 tgttaatggt gttcctgtaa atgaaactgc ttcaggaaaa gatcctatct gccctgtgca
     2461 ttcatcttct atgcaaaaac tattgcctga tgctcaagta atcccgtgtg atacggtcga
     2521 taatttgcag gcatctattg aaaatagtga atataaacag atcgttattg atgctaaaac
     2581 gcacgaagat ctctcaatga ttgcccaagt cataaatcac ataggtaata aaattattcc
     2641 tgttggctca gccggattgg cacagaagtt gaaaaatatc caaaatcaac aacaaccaga
     2701 gcgaaatctt aaattaggta gagcattaat tgtcgtaacc tcaattcacg aaacaagcca
     2761 actgcaagtt gatgaatata tttcaacttt aggaggaaaa tctattgtat ttaatccttc
     2821 gccttcacag ttgattaact actcgcaatc agaacattca ctgaaactac aattaaacgc
     2881 tctaatacgt tttagtagag atcatgttat tattcgggca aaccctgcaa aagttgtaaa
     2941 tgcaaaatct gccaatatca cggaaacttc taaagaaatt tctcaacatt tagctgattt
     3001 aagtaaattc tgccttgata atgaaaaatt tgatagcctt atattatttg gtggagatgg
     3061 tgcagcagca ttattagatc aaatgaatgt aacagagatg aatgtacaat atgcggtagt
     3121 accgggcgtt cctttatgct ccattgaaaa gggcaactac tcaggcatca ctgtaatgac
     3181 taaatctgga ggatttggaa acaacacact cttaagcaat ataatgaact agtaacaaca
     3241 aagaacctaa agcaacaatg t
//
LOCUS       DANLXS010000235         3228 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.240, whole genome shotgun sequence.
ACCESSION   DANLXS010000235 DANLXS010000000
VERSION     DANLXS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.240"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            82..534
                     /gene="iscR"
                     /locus_tag="PXD71_002482"
     CDS             82..534
                     /gene="iscR"
                     /locus_tag="PXD71_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDL6292543.1"
                     /translation="MKLTSKGRYAVTAILDIALHGKLEPVSLADISDRQAISLSYLEQ
                     LFAQLRRDGIVQSVRGPGGGYQLGKKPEEISVGMIISAVNENIDVTKCKGSGNCRKDS
                     QCLTHSLWERLEDQIEQFLGTISVADLAAEHADHDCEKEHCHDHPHHH"
     gene            589..1809
                     /locus_tag="PXD71_002483"
     CDS             589..1809
                     /locus_tag="PXD71_002483"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HDL6292544.1"
                     /translation="MKLPIYLDYAATTPMDERVAKKMMEYMTKDGIFGNPASRSHKFG
                     WEAEEAVDVARNQIADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKT
                     EHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLKAAIRPDTILVSVMHVNNEMGVI
                     QPIKEIGAICRAKKIIFHVDATQSVGKIEVNVQDLNVDLMSFSSHKLYGPKGIGGLYV
                     CRKPRVRLEAIIHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMARLTVLRDR
                     LYNGFKDIEEVYVNGSMEEGKRIGSNLNISFNFVEGESLMMSLRDIAVSSGSACTSAS
                     LEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDHTIEIVKKAIVKLRELSPLWEMF
                     KEGVDLSKIEWNHH"
     gene            1936..2319
                     /gene="iscU"
                     /locus_tag="PXD71_002484"
     CDS             1936..2319
                     /gene="iscU"
                     /locus_tag="PXD71_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDL6292545.1"
                     /translation="MAYSEKVIDHYENPRNVGTFDKEASDTGTGMVGAPACGDILRLQ
                     IKVNDQGIIEDARFKAYGCGSAIASSSLITEWVKGKSLEEAGAIKNSDIAEELELPPV
                     KVHCSILAEDAIKAAIADYKEKQAK"
     gene            2428..2751
                     /gene="iscA"
                     /locus_tag="PXD71_002485"
     CDS             2428..2751
                     /gene="iscA"
                     /locus_tag="PXD71_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDL6292546.1"
                     /translation="MSITLTEAAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEF
                     VDVLNEDDQVFEQHGVKVIVDEKSLTYLNGTQLDFVKEGLNEGFKYTNPNVKNECGCG
                     ESFSV"
     gene            2761..>3228
                     /gene="hscB"
                     /locus_tag="PXD71_002486"
     CDS             2761..>3228
                     /gene="hscB"
                     /locus_tag="PXD71_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HDL6292547.1"
                     /translation="MNNPFTLFDLPVQFQLDNAQLSERYLALQKQLHPDNFAHKSSAE
                     QLEAVQKSADVNEALNILKDPILRAEAMIEIHTGEAKNLEEKSMRDVDFLMQQLEWHE
                     KLETIEHRKDETELTVFLKQIKAEQKAVLEQLEQTLNTADWQKANALTDKLRYF"
BASE COUNT         1051 a          576 c          715 g          886 t
ORIGIN      
        1 atgattgact aaattggtgg ggtatatcaa aatttagcaa ctgtttcaga agattttctg
       61 gataatcaat gaggatattt tatgaagctt acctcaaaag ggcgttatgc agtaaccgcc
      121 attttagata ttgctttaca cggaaaacta gagccggtta gcttagccga tatttcagat
      181 cgccaagcga tttcactgtc ttatttagag caactgtttg ctcaacttcg ccgtgacggt
      241 atcgtgcaga gcgttcgtgg accgggtggt gggtatcaat taggtaaaaa accggaagag
      301 attagcgtgg gtatgatcat ctccgccgtg aatgaaaata ttgatgtcac caaatgtaaa
      361 ggtagtggta attgccgtaa agattctcag tgtttaacgc attctttgtg ggagcggtta
      421 gaagatcaaa ttgagcagtt tttagggaca atttcagttg cggatttagc cgcagaacac
      481 gctgatcacg attgtgaaaa agagcattgt cacgatcatc ctcatcatca ctaattgcaa
      541 attttttatc aaaaatgacc gcttgggttg ttttaactag gagtaacaat gaaattacca
      601 atttatttgg attacgcagc aaccacacca atggacgaac gtgttgcgaa aaaaatgatg
      661 gaatatatga ccaaagacgg tatctttggt aaccctgctt ctcgctcaca caaatttggt
      721 tgggaagcgg aagaagcggt tgatgtggct cgtaaccaaa ttgccgattt aatcggtgca
      781 gacagccgtg aaatcgtttt cacttctggt gcaacagagt cggataactt agccatcaaa
      841 ggtgcggctc atttctatca aaccaaaggt aaacacatta tcactgttaa aaccgaacat
      901 aaagcagtat tagatacttg ccgtcagtta gagcgtgaag gttttgaagt gacttactta
      961 gaccctgaag aagacggtat tttagattta gctaaattaa aagcggctat tcgtccggac
     1021 acaattttag tttcggtaat gcacgtaaat aacgaaatgg gcgtaatcca accgattaaa
     1081 gagatcggtg caatctgccg tgcgaagaaa atcattttcc acgttgatgc gacccagtct
     1141 gttggtaaaa tcgaagtgaa tgtacaagac ttaaatgttg atttaatgtc tttctcaagc
     1201 cacaaattat acggaccaaa aggtatcggc ggtctatatg tttgtcgtaa accacgtgtg
     1261 cgtttagaag caattattca cggtggcggt cacgagcgtg gtatgcgttc gggtacattg
     1321 cctgttcacc aaattgtagg tatgggtgaa gcatatcgta tcgctaaaga agaaatggca
     1381 acggaaatgg ctcgtttaac cgtgttacgt gatcgtttat ataacggctt taaagatatt
     1441 gaagaagtat atgtaaacgg ttcaatggaa gaaggcaaac gtatcggttc aaacctaaac
     1501 atcagcttta actttgttga gggcgaatcg ttaatgatgt cattacgtga tatcgcagta
     1561 tcatcaggtt cagcttgtac ttctgcaagt ttagagccat cttatgtgtt acgtgcaatc
     1621 ggtcgtgacg atgaattagc tcacagctca atccgtttca ctttaggtcg ttggacgact
     1681 gaagaagaaa tcgatcatac tattgaaatc gtgaaaaaag ctatcgtgaa attgcgtgag
     1741 ctttcgcctc tttgggaaat gttcaaagaa ggtgtagatt tgtctaaaat cgaatggaat
     1801 caccactaat tcagaaaaat tttgcaaggg caaataatat ttgcccctac aaaataagct
     1861 ttcatcacaa atagtaaatt gaatgtaggg gcgaaatagg tttcgccgga aataggaaat
     1921 aaaaggaata caaaaatggc atatagcgaa aaagtaattg atcattacga aaacccacgc
     1981 aacgtaggga cttttgataa agaagcatca gacaccggta caggtatggt gggagcaccg
     2041 gcttgtggtg atattttacg tttacaaatt aaagtgaacg atcaaggtat cattgaagat
     2101 gcacgcttta aagcatacgg ttgtggttct gcaatcgcat caagttctct cattaccgaa
     2161 tgggtaaaag gtaaatcatt agaggaagca ggtgcgatta aaaacagtga tatcgctgaa
     2221 gagttagaat taccaccggt aaaagttcac tgttcaatct tagcagaaga tgcaatcaaa
     2281 gctgcaattg cagactacaa agagaaacaa gctaagtaat atgatacaat caatgcaagc
     2341 ggtcgatttt tgcaaaaaat ttgcaaaatt taaccgcttg catttgctat ttctatcttg
     2401 atttaatcca ttcacataag gaaaactatg agcattacct taacagaagc tgccgcaaac
     2461 cgagttcgta cattcttaga aaacagaggt aaaggtatcg ggctaaggct tggtgtgaaa
     2521 acttccggtt gttcggggct tgcctatgtg cttgaatttg ttgatgtatt aaatgaagac
     2581 gatcaggttt tcgaacaaca cggcgtaaaa gttattgtcg atgaaaaaag cctgacttac
     2641 ttaaacggta cgcagctgga ttttgtgaaa gaaggcttaa acgaaggctt taaatacacc
     2701 aacccgaatg ttaaaaacga atgcggctgt ggcgaaagtt ttagtgtgta aggaactgta
     2761 atgaataacc cttttacttt atttgattta cccgttcaat ttcaacttga taacgcccag
     2821 ctttctgagc gttatcttgc tttacagaaa cagcttcacc cagataactt tgcccacaaa
     2881 tcaagtgctg aacagttaga agcggtgcaa aaatctgctg atgtcaatga ggcactcaat
     2941 attcttaaag atccgatttt acgggcagaa gcgatgattg aaatccatac aggtgaagcc
     3001 aaaaatctcg aagaaaaaag tatgcgagat gtggatttct taatgcagca actcgaatgg
     3061 cacgaaaaac tggaaaccat tgagcatcgt aaagacgaaa ccgaactgac cgtcttttta
     3121 aaacagatta aagcggaaca aaaagcagta ttagaacagc ttgaacaaac gttaaataca
     3181 gccgactggc aaaaagccaa tgcgttaacc gacaagctcc gttacttt
//
LOCUS       DANLXS010000236         3210 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.242, whole genome shotgun sequence.
ACCESSION   DANLXS010000236 DANLXS010000000
VERSION     DANLXS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.242"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(77..781)
                     /locus_tag="PXD71_002487"
     CDS             complement(77..781)
                     /locus_tag="PXD71_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDL6292548.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            complement(872..1699)
                     /locus_tag="PXD71_002488"
     CDS             complement(872..1699)
                     /locus_tag="PXD71_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034033256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-2"
                     /protein_id="HDL6292549.1"
                     /translation="MAIRIFAILFSIFSLATFAHAQEGTLERSDWRKFFSEFQAKGTI
                     VVADERQADRAMLVFDPVRSKKRYSPASTFKIPHTLFALDAGAVRDEFQIFRWDGVNR
                     GFAGHNQDQDLRSAMRNSTVWVYELFAKEIGDDKARRYLKKIDYGNADPSTSNGDYWI
                     EGSLAISAQEQIAFLRKLYRNELPFRVEHQRLVKDLMIVEAGRNWILRAKTGWEGRMG
                     WWVGWVEWPTGSVFFALNIDTPNRMDDLFKREAIVRAILRSIEALPPNPAVNSDAAR"
     gene            complement(1776..2567)
                     /locus_tag="PXD71_002489"
     CDS             complement(1776..2567)
                     /locus_tag="PXD71_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA25"
                     /protein_id="HDL6292550.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2625..3158)
                     /gene="aadB"
                     /locus_tag="PXD71_002490"
     CDS             complement(2625..3158)
                     /gene="aadB"
                     /locus_tag="PXD71_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="HDL6292551.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
BASE COUNT          736 a          908 c          788 g          778 t
ORIGIN      
        1 agtgccgatt gaccgagctt taacggcact gttgcaaagt tagcgatgag gcagcctttt
       61 gtcttattca aaggccttac atttcaaaaa ctctgcttac caggcgcatt tcgcccaggg
      121 gatcaccata ataaaatgct gaggcctggc ctttgcgtag tgcacgcatc acctcaatac
      181 ctttgatggt ggcgtaagcc gtcttcatgg atttaaatcc cagcgtggcg ttgattatcc
      241 gtttcagttt gccatgatcg cattcaatca cgttgttccg gtacttaatc tgtcggtgtt
      301 caacgtcaga cgggcaccgg ccttcgcgtt tgagcagagc aagcgcgcga ccataggcgg
      361 gcgctttatc cgtgttgatg aatcgtggga tctgccactt cttcacgttg ttgaggattt
      421 tacccagaaa ccggtatgca gctttgctgt tacgacggga ggagagataa aaatcgacag
      481 tgcggccccg gctgtcgacg gcccggtaca gatacgccca gcggccattg accttcacgt
      541 aggtttcatc catgtgccac gggcaaagat cggaagggtt acgccagtac cagcgcagcc
      601 gtttttccat ttcaggcgca taacgctgaa cccagcggta aatcgtggag tgatcgacat
      661 tcactccgcg ttcagccagc atctcctgca gctcacggta actgatgccg tatttgcagt
      721 accagcgtac ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca
      781 tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag
      841 tgcccgttga agtaaccggc gctgcgcggt tttatcgcgc agcgtccgag ttgactgccg
      901 ggttgggcgg taacgcttca atagagcgaa ggattgcccg cacgattgcc tccctcttga
      961 aaagatcatc cattctgttt ggcgtatcaa tattcagtgc gaagaatacg gagccagtcg
     1021 gccactcaac ccatcctacc caccaaccca tacggccttc ccagcccgtc tttgcacgca
     1081 gtatccagtt gcgaccggct tccacaatca tgagatcctt gaccaagcgc tgatgttcta
     1141 cccgaaaggg cagctcgtta cgatagagct tcctgagaaa tgcaatttgc tcctgcgccg
     1201 agattgcaag gctgccttct atccagtaat cgccattact tgtcgaagga tcggcgttgc
     1261 catagtcgat tttcttcaaa tagcgccgag ctttgtcatc accaatttcc tttgcaaata
     1321 gctcatacac ccaaacagta gaattccgca ttgctgatcg caaatcttgg tcttgattgt
     1381 ggcctgcaaa gcccctgtta acgccgtccc atcgaaaaat ctggaactca tcacgaacag
     1441 cgcctgcatc aagtgcaaaa agtgtatgag gtatcttgaa tgtcgatgca ggcgagtagc
     1501 gtttcttcga tcgcacagga tcaaaaacca acatggcacg atccgcttgg cgttcgtctg
     1561 ccacaactat cgtgcctttg gcttgaaatt cgctgaaaaa cttcctccag tcagaacgtt
     1621 ctagcgtgcc ttcttgcgca tgcgcgaaag tggcaagaga aaaaatggag aaaagtatcg
     1681 cgaagattcg gattgccatt aacttttcct taatgcccaa cggccaaggt aagccgcgcc
     1741 gcgaagcggc gtcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc
     1801 tcgcctttca cgtagtgaac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     1861 tcttcttgtc caagataagc ctgtctagct tcaagtaaga cgggctgata ctgggcaggt
     1921 aggcgtttta ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttattgcg
     1981 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
     2041 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     2101 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     2161 gtcagcaaga tagccagatc aatgtcgatc atggctggct cgaagatacc cgcaagaatg
     2221 tcattgcgct gccattctcc aaattgcagc tcgcgcttag ccggataacg ccacgggatg
     2281 atgtcgtcat gcacgacaag ggtgacttct atagcgcgga gcgtctcgct ctcgccaggg
     2341 aaagccgaag cctccataag atcattgagc aatgctcgcc gcgtcgtttc atcaagcttt
     2401 acggccacag taaccaacaa atcaatatcg ctgtatggct tcaggccgcc atccactgcg
     2461 gagccgtaca aatgcacggc cagcaacgtt gattccagat ggcgctcaat gacgcttagc
     2521 acctctgata gttggttcga aatttcgatg gtcaccgctt ccctcatgat gtctaacacc
     2581 tgagttaagc cgcgccgcga agcggcgtcg gcttggacga attgttaggc cgcatatcgc
     2641 gacctgaaag cggcacgcaa gacctcaacc ttttccgccc cgagtgaggt gcatgcgagc
     2701 ctgtaggact ctatgtgctt tgtaggccag tccactggtg gtacttcatc ggcatagtaa
     2761 aagtaatccc agatgatcgc ctcccagctg ttacaacgga ctggccgccc ggcgatgacg
     2821 ccctcagccg cctctgggca cgagccctgc ggagcctccg cgatttcata cgcttcgtct
     2881 gcccaccaag caggttcgca gtcaagtaac tcatccccga tctccgctaa gaatccatag
     2941 tccaactcct ccatgacgcg cccgccgagc atttcaacta ttgcctcgag ctcgccgcgc
     3001 ctctcgccgg gaaacgtcag atcaatatca tcgtgcttgc gtgttacacg ccctagccgt
     3061 gcatcgatcg cccagccccc accgatccag agcggcagat ttcgctcatc tgccgcagct
     3121 agaattttgt gtatcaatgt gacctgcgtt gtgtccatgc ggcctaacct ttgttttagg
     3181 gcgactgccc tgctgcgtaa catcgttgct
//
LOCUS       DANLXS010000237         3173 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.243, whole genome shotgun sequence.
ACCESSION   DANLXS010000237 DANLXS010000000
VERSION     DANLXS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.243"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(39..1874)
                     /gene="glmS"
                     /locus_tag="PXD71_002491"
     CDS             complement(39..1874)
                     /gene="glmS"
                     /locus_tag="PXD71_002491"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL6292552.1"
                     /translation="MCGIVGAVAQRDIAEILVDGLHRLEYRGYDSAGVAVLNADDKTM
                     QIVRRVGKVKALDEALDTKPLLGGTGIAHTRWATHGEPSETNAHPHRSGKIAVVHNGI
                     IENYEELKIVLQERGYVFQSQTDTEVIAHLVEWELRSASSLLEAVQKTVVQLRGAYGT
                     VVLNEEEPEHLIVARSGSPLVIGYGVGENFLASDPLALLSVTRRFAYLEEGDVAEITR
                     RTVDIYDREGNKAEREIHEGNFEADAADKGQYRHYMQKEIFEQPVAIMNTLDGRIKDG
                     KVDISAIAPNAAEILSKVEHVQIVACGTSYNAGMVSRYWFESIAGVSCDVEIASEFRY
                     RKFVTRPNSLLITLSQSGETADTLAALRLAKESGYMAAMTVCNVASSSLVRESDFAFL
                     TKAGVEIGVASTKAFTTQLTCLLLLNVAIGRLQGRMSEEQEHHIVQSLQRLPAQIESA
                     LVFDKQIEKLSEDFADKHHTLFLGRGEYYPVAMESALKLKEISYIHAEAYAAGELKHG
                     PLALIDSDMPVVVVAPENDLLEKVKSNIEEVRARGGQLYVFADHDAGFEEAEGFKTIV
                     MPKVDEVTAPIFYTVPLQLLSYHIALIKGTDVDQPRNLAKAVTVE"
     gene            complement(1922..2683)
                     /locus_tag="PXD71_002492"
     CDS             complement(1922..2683)
                     /locus_tag="PXD71_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL6292553.1"
                     /translation="MSKRNTQQRRHLIVQMVQEKNEVSVDELAHFFETSEVTIRKDLT
                     ALEESGFLLRKYGGAVKIPSEMMEEAFIEQLSIQKKMIAQAARDCIRDHNRIIIDSGS
                     TTGALVKALNQTGLVVMTNSLNLATELTSLECEPTVLMTGGTWDSRSESFQGKVAEQV
                     LRSYDFDQLFIGADGLDLARGTTTFNELVGLSQVMAEVSREVIVLIESQKIGRKMPNI
                     ELKWQNITKIITDSQLSFETKEQIEKMGVEVVIAK"
     gene            complement(2840..3040)
                     /locus_tag="PXD71_002493"
     CDS             complement(2840..3040)
                     /locus_tag="PXD71_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL6292554.1"
                     /translation="MKSILKVVGAMVLMVAVSGCNNMTKTQRNTAIGAAIGGVAGHAI
                     GESTGATLGGAALGGVIGSQIK"
BASE COUNT          892 a          752 c          554 g          975 t
ORIGIN      
        1 atataaaaac cccaagatat cttggggttt aactaacatt actctacagt caccgctttt
       61 gcaaggttac gaggttggtc aacgtccgtt cctttgatta aggcaatgtg atatgaaagt
      121 agttgtaacg gcacggtgta gaaaatcggt gcggttactt catcaacttt cggcattaca
      181 atcgttttga agccttctgc ttcttcaaaa cctgcatcgt gatcggcaaa cacatataac
      241 tgaccgcctc ttgcacgcac ttcttcaata ttggatttca ctttctcaag caagtcattt
      301 tccggtgcta ccaccactac cggcatatcg ctatcaatta atgctaacgg gccgtgtttt
      361 aactcgcctg cggcataagc ttctgcgtga atatatgaaa tctcttttaa tttcaatgca
      421 gattccatcg caaccgggta atattctcca cgacctaaga acaatgtgtg gtgtttatcc
      481 gcaaaatctt ccgataattt ttcgatttgt ttatcgaaca ctaacgcact ttcaatttgt
      541 gctggtaagc gttgtaaaga ttgcacgata tggtgttctt gctcttcact cattctgcct
      601 tgtaagcgtc caatcgccac atttaataac aataagcaag ttaattgagt agtgaatgct
      661 ttggttgatg ctacaccaat ttcaacacca gctttggtta agaatgcaaa atcggattca
      721 cgcactaatg aagaacttgc cacattacac accgtcattg ccgccatata gccagattct
      781 ttcgctaaac gcagtgcagc taacgtatct gcggtttcac ctgattgtga aagcgtgatt
      841 aataagctgt tcgggcgagt aacgaattta cggtaacgga attcggaggc aatttccaca
      901 tcacagctca cgcctgcaat ggattcaaac caataacgtg aaaccatacc agcattgtaa
      961 gaggttccac aagccacaat ttgcacgtgt tctactttcg ataaaatctc tgccgcattc
     1021 ggtgcaatgg ctgaaatatc tactttaccg tctttgatac gaccatctaa ggtattcata
     1081 atcgcaaccg gctgctcaaa aatctctttt tgcatatagt gacgatattg tcctttatct
     1141 gccgcgtctg cctcaaaatt gccttcgtga atttcacgct ctgccttgtt gccttcacgg
     1201 tcataaatat ccactgtgcg acgggtaatt tcggctacat caccttcttc tagataagca
     1261 aaacggcgag ttacgcttaa taatgcaagt gggtctgaag caaggaagtt ctcgcctaca
     1321 ccgtaaccaa tcactaacgg gctacctgag cgagccacaa ttaaatgctc cggctcttct
     1381 tcatttaata ccactgtgcc gtaagcacca cgcagttgca ccacggtttt ttgtaccgct
     1441 tccaataatg aagaagcaga acgcaattcc cattcaacta aatgagcaat gacttcggta
     1501 tcggtttgag attggaacac ataaccacgc tcttgtaaca ctatttttaa ctcttcgtag
     1561 ttttcgataa tcccgttatg aactactgca attttgccac tgcggtgtgg gtgagcatta
     1621 gtttctgacg gctcgccgtg cgttgcccag cgagtgtgtg cgatcccagt gccgcctaat
     1681 agtggcttag tgtctaacgc ctcatctaag gctttgactt taccgacacg gcgtacaatt
     1741 tgcattgttt tatcatctgc atttaatact gctacaccag cagagtcata tccacggtat
     1801 tctaagcgat gtaaaccatc aaccaagatt tctgcgatat cacgttgtgc tactgcacct
     1861 acaattccac acataaattt gctttcctat gtttaagttt aaaaattcat ttgagctact
     1921 gctattttgc aataaccact tcgacaccca ttttttcgat ttgttctttc gtttcaaaac
     1981 ttaattgact gtcggtaata attttggtaa tattttgcca tttcaattca atattcggca
     2041 ttttccgacc gattttttgc gattcaatta acacaatcac ttcacggctc acttctgcca
     2101 tcacctgact taagccaact aattcattaa aggtagtcgt gcctcgagcc aaatctaaac
     2161 cgtccgcacc aataaaaagc tgatcgaaat catatgaacg caatacctgt tctgctactt
     2221 tgccttgaaa ggactcagaa cggctatccc aagtgccgcc ggtcatcaac actgttggtt
     2281 cgcattcgag ggaagtcaat tccgtcgcta aattcagcga atttgtcatc accaccaagc
     2341 cggtttggtt caatgctttt accaaagctc cggtcgtgct gccgctgtca ataatgatgc
     2401 gattatgatc acgaatacaa tcacgtgcgg cttgagcaat cattttcttt tgaatcgaaa
     2461 gttgctcaat aaacgcttct tccatcattt ccgatggaat ttttaccgcc ccaccatact
     2521 tacgcaataa aaaaccactt tcttccaaag cggttaaatc ttttctaatc gtaacttcag
     2581 acgtctcaaa gaaatgagcg agttcatcta cactcacttc atttttctct tgtaccattt
     2641 gaacaattaa atggcggcgt tgttgcgtgt tacgttttga cattataatc ttcacttaaa
     2701 tttcgtttca aaacttttgc attataaaga aagctcgcga aaagaaaagc agaaaatgca
     2761 aaatttacaa aatctataca aaataaaacc gcttgtaata ttgatttaca agcggttgat
     2821 ttcttgaaat tttttgcaat tatttgattt ggctaccaat cacgccacca agtgcggcac
     2881 cacctaaagt tgcaccggta gattcaccaa tcgcgtgacc agccacaccg ccaattgctg
     2941 caccgattgc agtattacgt tgcgtttttg tcatattgtt acagccagac acagcaacca
     3001 ttaataccat tgcaccaaca actttcaaaa tagatttcat aataatccct cataattaat
     3061 caatttcaaa taagccaata ttcttggcta tgctataaga tttaatttat ttagaaaggt
     3121 tcaactacta ccacctatga attgcaaatt ttttcacaat ttaggccgct tgt
//
LOCUS       DANLXS010000238         3155 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.244, whole genome shotgun sequence.
ACCESSION   DANLXS010000238 DANLXS010000000
VERSION     DANLXS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.244"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..282)
                     /gene="ybgE"
                     /locus_tag="PXD71_002494"
     CDS             complement(<1..282)
                     /gene="ybgE"
                     /locus_tag="PXD71_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDL6292555.1"
                     /translation="MIHKLYNLTAKGLLKALSFILAAVLFATIWVNSTAFALSFGGKT
                     PYLAMLVFYGMAILWVHGIGFEIRAAIWKVIFLPLLGYLIVIPALWILLV"
     gene            complement(282..380)
                     /locus_tag="PXD71_002495"
     CDS             complement(282..380)
                     /locus_tag="PXD71_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CydX/CbdX family cytochrome bd oxidase small
                     subunit"
                     /protein_id="HDL6292556.1"
                     /translation="MFYVTWVLGVLLAIFFATVITISIEKTGKFDE"
     gene            complement(395..1528)
                     /gene="cydB"
                     /locus_tag="PXD71_002496"
     CDS             complement(395..1528)
                     /gene="cydB"
                     /locus_tag="PXD71_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDL6292557.1"
                     /translation="MIDYEILRFIWWILIAVLLIGFAITDGFDMGVLNLLPIIGKSNV
                     ERRIMINTIAPHWDGNQVWLLTAGGAIFAAWPTVYATSFSGFYIAMVLVLAALFFRPV
                     GFEYRAKIDNPKWRSAWDWGLFIGGFVPSLVFGVAFGNLLQGVPFEFNNINQSQYTGS
                     FFGLLNPFALVCGIISLMMLTTQGATWLQMKTTDELHNNSRKVAQVTASIVLVAFILA
                     GVWLYFKDGFVITSELDHNAQSLLTNKTVAVQAGAWFRNYAEMPVLWVVPALVVVGAL
                     LTIIASKANRSGLAFFSSSLMIVGVILTAAVSMFPFIMPSITHPEMSLTVWDATASHT
                     TLTVMLFVAGIFVPLVLAYTIWSYIKMYGRLDASYLEENKAAY"
     gene            complement(1541..3091)
                     /locus_tag="PXD71_002497"
     CDS             complement(1541..3091)
                     /locus_tag="PXD71_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL6292558.1"
                     /translation="MLDVVELSRLQFALTALYHFLFVPLTLGLSFVLVVMETLYVTTG
                     KEVYKDMTKFWGKLFGINFALGVTTGITMEFQFGMNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWERLSKAKHLLATYCVAFGSNFSALWILVANGWMQNPIASEFN
                     FETMRMELSSFSELVLNPVTQSKFLHTVTAGYVCGAIFVLGISSYYILKGRDLGFAKR
                     SFSVGASFGLIAIISVLIMGDESGYEIGKAQPTKLAAMEGEFETQPAPAAWNAFVIPN
                     TAEMKNEFSIHIPYAAGIIATRSLDKEIKGLKDIRVINEQRVRTGMVAYGLLEKLRSG
                     NYTEADKEAFKAVQKDLGFGLLLKQYTPNVVDATEEQIQKAADNTIPNVGPTFWAFRV
                     MMLSGFLMLILITTAFIQNMRGKVESSRFLLRGLLFGLPLPWIAIESGWFLAEYGRQP
                     WAIYEMLPVGVSNSALTTGDLWFTIGLLCGLYTLFLVVEMYLMFKYGRLGPSALKTGR
                     YYFEQSTK"
BASE COUNT         1128 a          653 c          508 g          866 t
ORIGIN      
        1 aacaagtaaa atccataatg ccggaataac aataaggtaa cctagaagtg gtaaaaaaat
       61 cactttccaa atagcagctc ggatttcaaa tccaattcca tgtacccata aaatagccat
      121 accataaaat accaacattg ctaaatacgg cgtttttcca ccaaaagaaa gagcgaaagc
      181 ggtcgaattt acccaaatag ttgcaaataa tacagccgct aaaataaaag aaagggcttt
      241 taacaagccc tttgctgtta agttatagag tttatgaatc attactcgtc aaacttacct
      301 gttttttcaa tgctgatcgt gatgactgtt gcaaagaaga ttgcaagcaa tacacctaag
      361 acccaagtta cataaaacat attgtgtctc cttattagta agctgcttta ttttcttcta
      421 aataactagc atctaaacga ccatacattt tgatatatga ccaaatagta tatgctaata
      481 ctaacggtac aaagatacca gcaacaaata acattacagt taatgttgtg tggctagcag
      541 tagcgtccca aaccgttaag ctcatttctg gatgagtaat tgacggcata atgaatggga
      601 acattgatac tgcagcagtt aaaatcaccc caacaatcat caatgatgat gagaagaatg
      661 ctaaacctga acgatttgct tttgaggcaa taatagtcag taatgcacct actacaacta
      721 atgcaggaac aacccataat acaggcattt cagcataatt tctaaaccat gcaccagctt
      781 gaaccgctac agttttattc gtcaatagag attgtgcatt atgatctaac tcgcttgtga
      841 tcacaaaacc atctttaaaa tataaccata cacctgcaag aataaatgcc actaatacaa
      901 tagaagctgt cacttgtgcc acttttcttg agttgttatg tagctcatca gtagttttca
      961 tttgtaacca agttgcaccc tgtgtggtta acatcattaa gctgataata ccgcacacta
     1021 atgcaaatgg atttaataaa ccaaagaatg aacccgtata ttgtgattgg ttaatattat
     1081 taaattcaaa tggtacacct tgtaataagt taccgaatgc aacaccgaat actaatgatg
     1141 gaacgaaacc accaataaat aatccccaat cccaagcact acgccatttc ggattatcaa
     1201 tttttgcacg atactcaaaa cctacagggc ggaaaaataa tgctgcaagc acaagcacca
     1261 ttgcaatata gaaacccgag aacgaagttg catagactgt tggccatgca gcgaagatcg
     1321 caccaccagc ggtcaataac catacttggt taccatccca gtgaggggca attgtgttga
     1381 tcataatacg acgttcaaca ttacttttac caatgatagg taaaaggttt aatacgccca
     1441 tatcaaaacc atcagtaatt gcaaagccaa ttaacaatac cgcaattaag atccaccaaa
     1501 taaagcggag aatttcataa tcgatcattt ctaactccta ttatttagtt gattgttcaa
     1561 aataataacg gcctgttttt aatgcactag ggccaagacg accatatttg aacattaaat
     1621 acatttctac aactaagaat agtgtatata aaccacacaa taagccgatt gtaaaccata
     1681 aatcacctgt tgttaatgca gagtttgaca caccgacagg taacatttca tagatagccc
     1741 aaggctgacg gccatattcg gcaaggaacc aaccagattc aatcgcaatc caaggtaacg
     1801 gaagaccgaa taacaagcct cttaataaga aacggctact ttctacttta ccacgcatat
     1861 tttgaataaa tgctgtcgta atcaagataa gcatcaagaa gccagataac atcattacac
     1921 ggaatgccca gaatgtaggg cctacatttg gaattgtatt atctgctgct ttttgaattt
     1981 gttcttcagt cgcatcaacc acattaggtg tatattgttt taacaataac ccaaagccta
     2041 aatctttttg tacagcttta aatgcttctt tatccgcctc tgtataattg ccagaacgta
     2101 atttttctaa tagtccgtag gctaccatac cggtacgaac acgttgttca ttaattacac
     2161 gaatatcttt taggccttta atttctttat ctaaagaacg ggttgcaata ataccagctg
     2221 cataaggaat atgaattgag aattcatttt tcatttccgc agtatttgga atcacaaaag
     2281 cattccaagc agctggagca ggctgagttt cgaattcacc ttccattgca gctaatttag
     2341 tcggctgagc tttaccgatt tcataaccag attcatcacc cataatcagt acagagatga
     2401 tcgcaattaa accaaaactt gcacctactg agaatgaacg ttttgcaaag cctaagtcac
     2461 gaccttttaa gatatagtat gaactaatac ctaatacaaa aatagcacca caaacatagc
     2521 ctgcggttac agtatgtaag aatttagatt gtgtaactgg gtttaataca agttcagaga
     2581 aacttgaaag ctccattcgc atggtttcaa agttgaattc agaagcaatc gggttttgca
     2641 tccaaccatt agcaactaaa atccatagtg cagagaagtt agaaccaaac gcaacacagt
     2701 aagttgctaa taaatgtttc gctttcgata aacgttccca gccaaagaag aacaagccca
     2761 caaaggtaga ttctaagaag aacgccatta aaccttcaat cgctaatgga gcgccaaaaa
     2821 tatcacctac ataatgagaa taatatgacc agttcatacc aaactggaat tccattgtaa
     2881 taccagttgt tacccctagg gcaaagttaa taccaaataa tttaccccag aatttagtca
     2941 tatctttata tacttcttta cccgtagtta catacagggt ttccatcacc acaaggacga
     3001 aagaaagacc taatgtaagc ggtacgaata agaagtgata gagtgcagtt agagcaaact
     3061 gcaaacgtga gagttcaact acatctaaca tctcaaacct cttatattaa tatactaatt
     3121 tgacaattag attactgtaa aagtaatcac ccaca
//
LOCUS       DANLXS010000239         3115 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.245, whole genome shotgun sequence.
ACCESSION   DANLXS010000239 DANLXS010000000
VERSION     DANLXS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.245"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..844
                     /locus_tag="PXD71_002498"
     CDS             <1..844
                     /locus_tag="PXD71_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6292559.1"
                     /translation="AGVLFACINTLIPKLTSISPIDSNVIALVQYLAAFIFLVPSMNN
                     IGFFSSLKTEHFGQHCFRIFLSAIGIQCWTMALANQLPIWQGIALLMTSPLFVTIGSG
                     LFLKEKVDKKRWIATAMGFVGAMIILEPWSDHFEMIALLPIAAAFFWAGYSLMVKKLS
                     RYDSPTTMVAYLFILITPFNLLIALTNLSPSGFSLPSWSDFGFLILLGFFTALAQLAV
                     AKAYSVADASYIQPFDFIKLPLNVLAGWLVFNWVPPGKLWLGAAIIIAATIYITHAET
                     KQSN"
     gene            complement(923..1414)
                     /gene="ilvN"
                     /locus_tag="PXD71_002499"
     CDS             complement(923..1414)
                     /gene="ilvN"
                     /locus_tag="PXD71_002499"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDL6292560.1"
                     /translation="MRRTLAVLLENESGALSRVVALFSQRGFNIESLTVAPTDDPSLS
                     RMTIVAQGEEQVLEQIEKQLHKLIDVFKVSNLSAYEHVEREVLLVKVRATGSSRDELK
                     RMVDIYRGQIVDLTPKLYTIQLSGSTEKLNAFIEAVKEETTIVEIVRSGVISLSRGDK
                     NAL"
     gene            complement(1407..3104)
                     /locus_tag="PXD71_002500"
     CDS             complement(1407..3104)
                     /locus_tag="PXD71_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDL6292561.1"
                     /translation="MIVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLGLNHVLVRHEQG
                     AVHMADGYARSTGKVGCVLVTSGPGATNTITGILTAYTDSVPLVILSGQVPSSLIGTD
                     AFQESDMIGISRPVVKHSFLVKNTEEIPEIIKKAFYIASTGRPGPVVIDIPKDVVNPL
                     NKYPYEYPQEVSMRSYNPTVQGHKGQIKKALKALLVAKRPVLYVGGGVISAEGSPEIT
                     EFAQKLNLPVTCSLMGLGAYPASDKQYLGMLGMHGTFEANNAMHESDLILGIGVRFDD
                     RTTNNLAKYCPNAKVIHVDIDPASISKTVQAYIPIVGSAKDVMEEFLSLLGEENLAKN
                     QVDLTAWWQQIDAWKARNCLAFEENSDVIKPQQVIRMIHKLTNGEAYVASDVGQHQMF
                     AALHYGFDKPRRWINSGGAGTMGFGLPAAMGVKFAHPDATVVCVTGDGSIQMNIQELS
                     TAKQYDTPIVIVSLNNRFLGMVKQWQDIIYSGRHSQVYMNSLPNFAKLAEAYGHVGIQ
                     IDHPSELEAKLTEAFSIKDKLVFVDVLVDETEHVYPMQVRGGAMNEMMLSKTERTDA"
BASE COUNT          856 a          682 c          589 g          988 t
ORIGIN      
        1 tgccggtgtg ttatttgcct gtatcaatac cttaattccc aaattaacgt ccatttcacc
       61 gattgactcg aacgtgattg cattggtgca atatttagct gcctttattt tcctcgttcc
      121 cagcatgaat aatatcggtt tcttctcttc attaaaaacc gagcattttg ggcaacactg
      181 tttccgtatt tttctatctg cgattggtat tcaatgttgg acaatggcac tcgctaacca
      241 attacctatt tggcagggaa ttgcgttatt gatgacttct ccattgtttg tgaccatcgg
      301 ctccggtctg tttttgaaag agaaagtgga taaaaagcgt tggattgcaa ccgcaatggg
      361 gtttgttggg gcgatgatta ttctagagcc ttggagcgat cattttgaaa tgattgcgtt
      421 actgccgatt gccgcggctt ttttctgggc aggttattcg ctaatggtga aaaaactctc
      481 tcgttacgat agcccaacta cgatggtcgc ttacctgttt attctaatta cgccttttaa
      541 tttgttgatt gcactcacga accttagccc tagtgggttt agtctgcctt cttggagcga
      601 tttcggcttt ttaatcttac ttggtttctt tacggcgtta gcgcaactgg cggtagccaa
      661 agcctacagt gtagcagatg cttcctacat tcagccgttt gattttatta aattaccttt
      721 gaacgtatta gccggttggc ttgtatttaa ctgggtgcca ccgggtaaat tatggctagg
      781 agctgcgatt attattgctg cgaccattta tattactcac gctgaaacta aacagagtaa
      841 ttagcaacaa attcacgcta cataaaaatc cctttatctc aaagataaag ggatttgcaa
      901 attttaagct aaaattgacc gcttacagag cgtttttatc gccacgagat agactaatca
      961 cgccagagcg aacaatctct acaatcgttg tttcttcttt aactgcctca ataaatgcat
     1021 tgagcttctc ggtagaaccg gataattgaa tggtgtaaag tttcggtgtt aaatctacga
     1081 tttgcccacg gtaaatatct accatacgtt tcagttcatc acgagatgaa cctgtagctc
     1141 gcacttttac caataacact tcacgctcaa cgtgttcata agcacttaag ttactcactt
     1201 taaacacatc aatgagtttg tgtagttgct tttcgatttg ttctaacacc tgttcttcgc
     1261 cttgcgcgac aattgtcata cgggaaaggc ttggatcatc agtaggggca acggtcaagc
     1321 tttcaatgtt aaaaccacgc tgcgagaaaa gggcaactac acgagataat gcacctgatt
     1381 cattttctaa taatacggct aatgttctac gcatctgttc tctccgtttt acttaacatc
     1441 atttcattca ttgctcctcc acgcacttgc attgggtaaa catgttccgt ttcatcgacc
     1501 aatacatcaa caaatacaag cttatcttta attgagaatg cctcagttaa ttttgcctcc
     1561 aattcagaag ggtggtcgat ttgaatccca acgtggccat aagcctctgc taatttagca
     1621 aagttaggta gagaattcat atacacctgt gaatggcggc cagagtaaat aatatcttgc
     1681 cactgtttca ccatacctaa gaaacggtta tttaagctga caatcacaat cggtgtgtcg
     1741 tattgctttg cggtggaaag ttcctggata ttcatttgga tactaccatc accggttaca
     1801 caaacaacag tggcatcagg gtgggcaaat tttaccccca tcgcagcagg taagccaaag
     1861 cccattgtgc ctgcaccgcc tgaattaatc caacgacgtg gtttatcgaa cccataatgc
     1921 aatgcagcaa acatttgatg ttgtccaaca tctgaggcaa cataagcctc cccattggtc
     1981 aacttgtgga tcattctaat cacttgttgt ggcttaatca catcactgtt ttcttcaaag
     2041 gctaagcaat tacgagcttt ccaagcatca atttgttgcc accaagcggt taaatctacc
     2101 tgattttttg caagattttc ctcacctaat aaagataaaa attcttccat tacatcctta
     2161 gcactgccta caattggaat ataagcttgt actgttttgg aaattgaggc tggatcaata
     2221 tccacgtgga tcacttttgc attcgggcaa tattttgcta agttatttgt tgtacgatca
     2281 tcaaaacgta ccccaatacc taaaatcaaa tcactttcgt gcattgcatt atttgcctca
     2341 aaagtgccgt gcatgcctaa catacctaaa tattgcttat cagaggcagg ataagcacct
     2401 aatcccatta atgaacaggt tacaggtaga tttagctttt gtgcaaactc tgtaatttct
     2461 ggcgaaccct ctgcactaat tacaccaccg cctacataaa gcactggacg ttttgctact
     2521 aatagtgctt ttaatgcttt tttaatttgc cctttatgcc cttgaactgt tgggttataa
     2581 gaacgcattg atacttcttg cggatattca tagggatatt tatttaatgg attgactaca
     2641 tctttaggaa tatcaataac taccgggcct ggtcgaccgg ttgatgcaat ataaaaggct
     2701 tttttgataa tttcaggaat ttcttctgta ttttttacta agaaactgtg ttttacaacc
     2761 ggacgagaaa tgccgatcat atcactttct tggaatgcat ctgtcccaat taaacttgat
     2821 ggaacttggc ccgacagtat tactaacgga acagagtcgg tataagcagt taaaatacca
     2881 gtaattgtat tcgttgctcc cggacctgaa gtcactagca cacagccgac tttacctgtt
     2941 gaacgggcat agccatcagc catatgtaca gcaccttgct cgtgtcgaac taaaacgtga
     3001 ttcaatccaa gtgtatgaat tgcatcataa atatcgagta cagagccacc cggatagccg
     3061 aaaacgtatt ctacgccttc atcctttaag gactgtacaa tcatttctgc accag
//
LOCUS       DANLXS010000240         2985 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.246, whole genome shotgun sequence.
ACCESSION   DANLXS010000240 DANLXS010000000
VERSION     DANLXS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.246"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..215
                     /locus_tag="PXD71_002501"
     CDS             <1..215
                     /locus_tag="PXD71_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1414 domain-containing protein"
                     /protein_id="HDL6292562.1"
                     /translation="KSKYHTKQFEQLLADLIATIEKHKAPVDLSLMALGNMITNILIE
                     NIQSPAQREALADAFADALKQSLKTK"
     gene            220..1953
                     /locus_tag="PXD71_002502"
     CDS             220..1953
                     /locus_tag="PXD71_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HDL6292563.1"
                     /translation="MFARLRSLLPTNSRQYREETSQKITWGHWFALFNIVLALLISSR
                     YAFNADWPNTLVGKLYFFVSLFGHFSFVVFAGFLLILFPLSFIVKNHRTYRGLSVILA
                     TIGQTVLLVDTEVFKQFYLHLSPLVWDLLVNPEEGELTRKWQLLFVPMPLLLLAEMLY
                     SRWCWQKLRSFSRQKWGKYVALFFVISFTATHLIYAWADMNAYRPITAQRANYPLSYP
                     MTARNFLEKHGFIDRVEFNQEIEESGRLDTFYLNYPKSPLIATKTPSTNILLINLSGL
                     SKQIINAETMPKLTEISQQSHRFMQHYSSGDSASAGALGLFYSLSGKYLDSVLADKTV
                     SPLIEIARKSDYQLGLFSHNGFADPIYHQAIFSGILLPTAHSNVGAIGQWKNWISSRS
                     LENPFFSYLDLRMPFAHTAQEQAKLLDWQFAEIWQHLTQNALLANTLVIITADISNEV
                     GAENSFDKQHIQVPMMFYWQGESKEYHFLSSHLDIMPTLLSQFFGVQNPLNDYAQGMD
                     LSTQNQRLWVSSSNHKWNVAIMPNGDQYQIDRKGNFKHFNSNGEHEKDARPPLALFLQ
                     MIQQSNQFIEK"
     gene            complement(2020..2721)
                     /locus_tag="PXD71_002503"
     CDS             complement(2020..2721)
                     /locus_tag="PXD71_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6292564.1"
                     /translation="MIGQALGWRATFGIIGIIALIVLMMIWRLLPYLPSKNAGSIKSL
                     PLLFKRPMLIGIYLLVALIVSGHFTAYSYIEPFMIKISSVSVENTTFILLVFGLAGVV
                     ASFLFGRLYGKAPNKFILSGIAIIMCSQLCLLFLSDSTVAMFMVAFVWGIGITGMSIA
                     LQMKVLELAPDATDVATAIFSGTYNLGIGGGALLGSIAIQQLGLANIGFAGGSIAFVA
                     MVWFVFVSLKYRQLR"
     gene            complement(2718..>2985)
                     /locus_tag="PXD71_002504"
     CDS             complement(2718..>2985)
                     /locus_tag="PXD71_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6292565.1"
                     /translation="KLARKAQRRLSFLRVFSLAFAAFIVNTTEFIPIALLSDIASSFQ
                     MEPSSAGIMMTVYAWVVFLLSLPLMLLTANMERKRLLLMLFMGE"
BASE COUNT          933 a          683 c          564 g          805 t
ORIGIN      
        1 cgaaatctaa ataccacacc aaacaatttg agcagctatt ggctgacctc atcgccacca
       61 tagaaaagca taaagcaccg gtggatttat cgttaatggc attaggcaat atgataacta
      121 acattttaat tgaaaatatt caaagcccgg cacaaagaga agctctcgct gatgcctttg
      181 ccgatgcatt aaaacaatca ctaaaaacca aataagatga tgtttgcacg cctacgcagt
      241 ttattgccga ccaattctcg ccaatatcga gaagaaacct cacagaaaat cacgtggggg
      301 cattggtttg cactatttaa tattgtgttg gcactactca tttcctctcg ttacgcattt
      361 aatgcagact ggccgaacac gcttgtaggc aaactttatt tctttgtcag cctgttcggg
      421 catttcagct ttgtggtctt tgcaggattt ctattaattc tgtttccgct cagttttatt
      481 gttaagaatc atcgcaccta tcgtgggctt tccgtgattt tagccaccat cgggcaaacc
      541 gtgttattgg tggatactga agtcttcaag caattttatc tgcatttatc accgcttgta
      601 tgggatctgt tagttaatcc ggaagaaggg gaactgaccc gtaaatggca actgctgttt
      661 gtaccaatgc cactcctttt gctggcagaa atgctctact ctcgctggtg ctggcagaaa
      721 ctgcgtagtt ttagccgcca aaaatggggc aaatatgtcg ctctcttttt cgtgatcagc
      781 tttaccgcta ctcatttaat ctatgcgtgg gcagatatga atgcctaccg cccgattacc
      841 gctcaacgag caaactaccc actttcttac ccgatgacgg ctcgtaattt cttagaaaaa
      901 cacggtttta ttgaccgagt agaatttaat caagaaattg aagaaagtgg cagactagat
      961 actttctatc tgaattatcc aaaatctccc ttgattgcga ccaaaacgcc ctcaactaat
     1021 attttactga ttaatttatc aggtttatct aaacagataa taaatgccga aacaatgcct
     1081 aagctgacag aaatcagcca acaaagccac cgctttatgc agcattacag tagtggtgat
     1141 tcagcctctg ccggtgcatt aggtctattt tacagtttaa gcggtaaata tttggattcc
     1201 gttttagccg ataaaaccgt gtcaccgctt atcgaaatcg ctagaaaatc ggactatcaa
     1261 ttagggctgt tttcacataa tggttttgcc gacccgattt atcatcaggc gatcttctct
     1321 ggtattttat tgcctacggc gcacagtaat gtaggggcga ttgggcaatg gaaaaactgg
     1381 atcagctctc gctcattgga aaatccgttc tttagctatt tagacttaag aatgcccttc
     1441 gcccacaccg ctcaagagca ggcaaaatta ttagattggc aattcgctga aatctggcaa
     1501 catttgaccc aaaatgcact attggcgaat acgctggtaa tcattaccgc cgatattagt
     1561 aatgaagttg gagcagaaaa tagcttcgat aaacaacaca ttcaagtgcc gatgatgttc
     1621 tactggcaag gcgaaagcaa agagtaccat ttcctttcaa gccatttaga tattatgcca
     1681 accttactga gccaattctt cggcgtgcaa aatccgctga atgattatgc acaaggtatg
     1741 gatttaagca cccagaacca acgtttatgg gtatccagtt ctaatcacaa atggaatgtg
     1801 gcgattatgc cgaatggcga tcaatatcaa attgatcgta aaggcaactt taagcacttt
     1861 aacagcaatg gcgaacacga gaaagatgct cgaccaccat tagcgttgtt cttacaaatg
     1921 attcaacaaa gcaatcaatt tattgaaaag taatgttcaa caagcggtca tttttgcagg
     1981 aaaatttgca aaatttcagc aaaatatgac cgcttgtcac tatctgagct gtctatattt
     2041 caagctaaca aatacaaacc aaaccattgc tacaaaagca atactcccac cggcaaatcc
     2101 gatattggct aatcccagtt gctgaatagc gatgctcccc agtaatgctc caccgccaat
     2161 gcctaaatta taagtgccgg aaaaaattgc agttgcgaca tcagtcgcat caggggcaag
     2221 ttcaagcacc ttcatttgca acgcaatgct cattccggta atgccaattc cccagacaaa
     2281 agcgaccata aacattgcca ctgttgagtc actcaaaaac agtaaacaaa gctggctgca
     2341 cataataatg gcgatcccgc ttaagataaa tttattcggg gctttgccat ataaccgacc
     2401 aaataaaaaa cttgccacca cacctgccaa gccaaaaacc agcaaaataa aggtggtgtt
     2461 ttccacactc accgaactga ttttaatcat aaacggctca atatagctgt aagcggtaaa
     2521 atgtcctgaa acgatcaatg ccaccagcaa ataaatgccg attaacatcg ggcgtttaaa
     2581 taacaaaggc aaacttttta tcgagcctgc atttttgctt ggtaaataag gcagcaaccg
     2641 ccaaatcatc attagcacaa tcaaagcgat aatcccaata atgccaaagg ttgcccgcca
     2701 gcctaaggct tgcccgatca ttcgcccata aataacatca gcaacagccg ttttctttcc
     2761 atattcgccg tgagtaacat caacggtaac gataataaaa ataccaccca cgcataaacc
     2821 gtcatcataa tgcctgccga tgaaggctcc atttggaaac tgcttgcaat gtctgaaagc
     2881 aatgcaatag gaataaattc ggtggtgttt actataaaag ccgcaaaggc aagactaaat
     2941 actctaagga acgataatct ccgttgagct tttcgagcaa gtttc
//
LOCUS       DANLXS010000241         2926 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.247, whole genome shotgun sequence.
ACCESSION   DANLXS010000241 DANLXS010000000
VERSION     DANLXS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.247"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(31..821)
                     /locus_tag="PXD71_002505"
                     /pseudo
     CDS             complement(31..821)
                     /locus_tag="PXD71_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(818..1519)
                     /gene="lpxH"
                     /locus_tag="PXD71_002506"
     CDS             complement(818..1519)
                     /gene="lpxH"
                     /locus_tag="PXD71_002506"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDL6292566.1"
                     /translation="MTYYFIADLHLNENQPEITHLFLQFMQEKALLAEAVYILGDLFD
                     FWIGDDEQSELISTVKQAIKTLTTSGTKCYFICGNRDFLIGKRFSEQTGMVILPDYHV
                     IDLFGTSTLLCHGDTLCTDDVKYQQFRKKVHQKWRQAIFLMFPLSWRIRIAEKIRSKS
                     KQEKRDKSAEIMDVNAEVTAQIVAKYNTTQLIHGHTHRQAIHQHNGFTRIVLGDWKEN
                     YASILSVTEQGAEFI"
     gene            complement(1594..2115)
                     /locus_tag="PXD71_002507"
     CDS             complement(1594..2115)
                     /locus_tag="PXD71_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HDL6292567.1"
                     /translation="MRAILKYFLILVSLSFFIVLGGAVNYAMPSYEDTVVTGMEVRRM
                     DKDGIISKANPADGEVRDVYFLFTEDPETKKVMVYRNEDTGWGLPPYFKFGSADIQAK
                     AQAYANEKQRVQIKYYGWRINWLNEFRNIVSIKPLEEAETVSKPIMSYVLYAVLAFLF
                     FLSVQFIRGIFKD"
     gene            complement(2125..2913)
                     /locus_tag="PXD71_002508"
     CDS             complement(2125..2913)
                     /locus_tag="PXD71_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HDL6292568.1"
                     /translation="MKDLFIIDSHCHLDSLDYETRHKNVDEVIENAKARGVHHFISVC
                     TTVGRFEAMKNLTAHRNDVSLSCGVHPLNVEDEPFDYDKLFQFAQDPKVVAIGETGLD
                     YHYTPETKALQQSLFVQQIEIANKLNKPLIIHSRSARQDTMDMLEQNNAEKCGGVLHC
                     FTEDWAMAKRALDIGFYISISGIITFRNAEELREVVRKVPLDRLLVETDSPYLAPIPY
                     RGKPNQPAYVRETCEYVATLKGVSTEELARITTENVQHLFNIKL"
BASE COUNT          858 a          585 c          558 g          925 t
ORIGIN      
        1 tttttttagg gaaaatctgc aaatgctttt ttagctccat tcaagcgtat aaggcttaat
       61 accaatccct ttactattaa gacagtcttg tagatttcta atattattaa caatattttc
      121 ggtaattagg ttggcttgtt taactttttc ttcagtaaca ttgggatctt tttcgttgaa
      181 taaaatcgca tttaataatc tttctgcgtg cataccctct cggcagtaat gtaaaacacc
      241 gacatcatta aatgcagtag ctacagagct aatttgtgta gtggctatgc ctaaagcacc
      301 atcttctcca cctaattctt ggaagagctg atgttgtgga aagccgccac aagcgaggaa
      361 gaaactacgc cgaaatacag tgttgcctgc accagtcatt tgcatatatt gccaagctaa
      421 ctcaaaatta gggtgagagc tgtaacgttg atctaagtta ccggttttaa tgctaaccga
      481 accaagccgg tttccggttt aaactcaaaa atcttttcag caacttgcaa tgccccgttt
      541 tcataagcat catcagcatc taaaaaggca attaaatctg tttcactttg taatgctccc
      601 cagttgcgtg ctttggcaac accgccattt tgaggcattt gttccactct aattttatca
      661 ggcacttggg cttgtaaatg tttagctaag gcaagcgtat tatccgttga agcatcatca
      721 ataagccaca atagatttaa tccagactga tttaatacac tttgcactgc acgaacaagg
      781 gtgttttcgg cgttatagca aggaataatc acattaatca tataaattca gctccttgct
      841 cggttacgct taaaatagag gcgtaatttt ctttccagtc gcctaataca atgcgggtaa
      901 atccattatg ctgatggatt gcttgtcgat gcgtatgccc gtgtataagt tgagttgtat
      961 tgtattttgc gacaatttga gcggtaactt cagcgtttac gtccataatt tcagcggatt
     1021 tatcacgctt ttcttgtttg cttttggatc gaattttctc cgcaatacga attcgccaag
     1081 aaagcggaaa catcaaaaag atagcttgtc gccatttttg gtggactttt ttacgaaatt
     1141 gttgatattt cacatcatca gtacaaagcg tatcgccgtg gcaaagcaag gtagatgtgc
     1201 cgaataaatc tataacgtga taatcaggca aaataaccat acccgtttgt tcgctgaatc
     1261 gtttgccaat taaaaaatca cgatttccgc aaataaaata gcattttgta ccgcttgtag
     1321 tgagggtttt gattgcctgt ttaacggttg aaatgagttc agactgctca tcatcgccaa
     1381 tccagaaatc aaacagatca cctaagatat aaaccgcttc tgcaagtagt gctttttctt
     1441 gcataaattg caaaaagagg tgggtaattt caggctgatt ttcatttaag tggagatcgg
     1501 caataaaata gtacgtcatt tgatattgtt gataaaaaag aaaacggttc aattttttac
     1561 aaaatcgaac cgtttgtcaa aggttaatcg gcattaatct ttaaaaatgc cacgaataaa
     1621 ttgtactgag aggaagaaca agaacgccaa tacggcataa agtacatagc tcataattgg
     1681 tttgctgact gtttctgctt cttctaatgg cttgattgaa acaatatttc taaattcatt
     1741 tagccaattg attcgccagc cgtaatattt aatttgcaca cgttgttttt cgttagcgta
     1801 ggcttgagct ttggcttgaa tgtcggcaga tccgaatttg aaataaggag gcaaccccca
     1861 gccggtatct tcattacgat aaaccattac ctttttggtt tcaggatctt ccgtgaataa
     1921 gaaataaaca tcgcgcacct cgccatcagc aggattcgct ttgctgataa taccatcttt
     1981 atccattcgg cgtacttcca tacctgttac tacggtatct tcataacttg gcattgcata
     2041 attaactgca ccgcctaata caataaaaaa tgatagtgag acaagaatca gaaaatactt
     2101 aagtattgct ctcatattag ttccttataa tttaatgttg aataagtgtt gcacattttc
     2161 ggttgtaatg cgagctaatt cttcagttga aacgcctttt aatgtcgcca catattcgca
     2221 agtttcccgc acatacgccg gctggttcgg cttaccacga taaggaatcg gggcaagata
     2281 aggggaatct gtttcaacca ataagcgatc taacggcact tttcgcacga cttctcggag
     2341 ttcttctgca ttgcggaaag taataatgcc cgaaattgag atataaaagc cgatgtctaa
     2401 agcccgtttt gccattgccc aatcttcggt aaagcaatgc aacaccccac cgcatttttc
     2461 ggcattattt tgttccaaca tatccatcgt atcttggcgt gccgaacgag aatggataat
     2521 gagtggctta ttgagtttgt ttgcaatttc aatttgttga acgaaaagcg attgttgcaa
     2581 cgctttggtt tccggtgtgt agtgataatc caaaccagtt tccccaatgg caaccacttt
     2641 cggatcttga gcgaactgaa ataatttatc gtaatcaaaa ggttcatctt ccacattcaa
     2701 cgggtgaacg ccacaagaaa gtgaaacgtc attacgatgg gcggttaagt ttttcattgc
     2761 ttcaaaacgc ccaactgtag tgcaaaccga gataaaatga tgcacccctc gggctttggc
     2821 attttcaatt acttcgtcca cgtttttgtg acgagtttcg tagtctagag aatctagatg
     2881 acaatgtgag tctatgataa ataagtcttt catttttcta aatata
//
LOCUS       DANLXS010000242         2918 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.248, whole genome shotgun sequence.
ACCESSION   DANLXS010000242 DANLXS010000000
VERSION     DANLXS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.248"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1178)
                     /gene="tolA"
                     /locus_tag="PXD71_002509"
     CDS             complement(<1..1178)
                     /gene="tolA"
                     /locus_tag="PXD71_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDL6292569.1"
                     /translation="MKSQQDRLELAVIVSILLHILLIGLLLLGSLFTKTELAAAGGSG
                     GDSDSFEAVMVDTGQVAAEYGQLLAEKKGSKASKPKVEPKEEAVEEDVEDKPTPEEIQ
                     KQKQAELAAIQEKQRQQEIERQQKLQEQQKQEQAKQEELKKQQAEEATRKKAAEAAKL
                     KADAEAKRLEAAAKQAEEEKKAKEALEKKLEQQKKAEAEKKLKEQKELKEQQAKEAKE
                     AKEKAEKEAKAKAEKEAKEKAEKEAKAKAEKEAKEKAEKEAKAKAAAEAKAAQAKNNK
                     ALDDFLNSGDIGGGSSKGGNKNSAGSQGSGGTSGLGQGTNVDGNAYGQRIKKLIQSRY
                     RVDPSFAGKQCDVKIFLSRDGTITNYQVISGDKAVCDAAVSAIVATRKVPPAPSDAVY
                     "
     gene            complement(1192..1629)
                     /gene="tolR"
                     /locus_tag="PXD71_002510"
     CDS             complement(1192..1629)
                     /gene="tolR"
                     /locus_tag="PXD71_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDL6292570.1"
                     /translation="MSRRLKRNDIKSEINIVPFLDVLLVLLLIFMATAPIISQSVEVN
                     LPEDTHSQSVSNEDKTPVILEVAGVGEYKLKIDGNYVQSNGQENINESEVVAYAGEQF
                     QKDNNTLFLVAGDKVVPYEEIMKGITLLKDAGVKSVGLMTEGK"
     gene            complement(1771..2451)
                     /gene="tolQ"
                     /locus_tag="PXD71_002511"
     CDS             complement(1771..2451)
                     /gene="tolQ"
                     /locus_tag="PXD71_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HDL6292571.1"
                     /translation="MQTEFSLLSLFLEASIVVKIVMLILIVFSILSWAVIIQRSKILT
                     KAKKDSLAFEDRFWSGEDLNRLHQGLENRRDALNGSEQIFYVGFKEFTRLQQANPDAP
                     ESIIQGSTRAMNLALNREMESLENYIPFLGTVGSISPYIGLFGTVWGIMHSFMGLSAV
                     KQATLQSVAPGIAEALIATAIGLFAAIPAVMAYNRLNLRLGKVEQGYVNFIDEFTTLL
                     HRQAFSKR"
     gene            complement(2473..2874)
                     /gene="ybgC"
                     /locus_tag="PXD71_002512"
     CDS             complement(2473..2874)
                     /gene="ybgC"
                     /locus_tag="PXD71_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDL6292572.1"
                     /translation="MDFPIRVYYEDTDAGGVVYHSNYLNFFERARTEFLRQQHFSQQE
                     LFSQSLAFVVKRIEIDYKLPARLDDLLNVETTLSELKKATLIFKQRLWKDGDCLSEAN
                     VTVACVDLIKMKPVAIPDDIRQALQKSYFSI"
BASE COUNT          769 a          679 c          459 g         1011 t
ORIGIN      
        1 ttatataccg catctgatgg tgcaggtggt acttttctag ttgccactat tgcacttacc
       61 gctgcatcac atactgcttt atcgccactg ataacttgat agttagtaat tgtgccatct
      121 cgacttaaga atatcttaac atcacattgt tttccggcaa aacttggatc aacccgatat
      181 cgagattgaa ttaatttctt aattcgttga ccatatgcat taccatctac attagtacct
      241 tgccctaagc cacttgtacc accagaacct tgtgatcctg ctgagttttt gttaccacct
      301 ttacttgaac caccaccgat gtctccacta ttgaggaaat catcaagtgc tttattgttt
      361 ttcgcttggg cggcttttgc ctcagcagct gccttcgctt tagcttcttt ctcggctttc
      421 tctttggctt ctttttctgc ttttgctttg gcttctttct cagccttttc ttttgcttct
      481 ttttctgcct tggctttagc ttctttttct gctttttcct tcgcttcttt agcttctttt
      541 gcctgctgtt ccttcagttc tttttgctct tttaactttt tctcagcttc cgctttcttc
      601 tgctgttcaa gcttcttttc aagtgcttct ttagcttttt tctcttcttc agcttgttta
      661 gcagcagcct ctaaacgctt agcttctgca tctgctttca gttttgctgc ttcagctgct
      721 tttttacgag ttgcttcttc tgcttgctgc tttttaagct cttcttgctt agcttgttct
      781 tgcttttgct gttcttgtag tttttgctga cgctcaattt cttgctgacg ctgtttctct
      841 tgaatcgccg ctaactcagc ttgtttctgt ttttggatct cttccggagt cggtttatct
      901 tccacatctt cttcaacagc ttcctcttta ggctctacct ttggcttcga tgcttttgag
      961 ccttttttct ctgccaataa ctgcccatat tcagcagcca cttgcccagt atctaccatc
     1021 acagcttcaa agctatcgct atcgccacca ctgccaccag ccgcagcgag ttccgtctta
     1081 gtgaaaagag acccgagcaa taacaacccg attaacaata tatgcaataa gatcgaaaca
     1141 ataaccgcta gctctagtct atcttgttga gatttcacac gttatccttg gctatttacc
     1201 ttctgtcatt aaacctaccg atttcactcc ggcgtcttta agtagtgtaa tacctttcat
     1261 aatttcttca taaggtacta ctttatcacc ggcaactaaa aataaggtat tgttatcttt
     1321 ttgaaattgt tcccctgcat aggcaacaac ttctgattca ttaatattct cttgcccatt
     1381 actttgcaca tagttaccgt caattttcaa tttatattca ccaacacctg ccacctccaa
     1441 aatcactgga gttttatctt cattagaaac tgattggcta tgcgtatctt ccggtaaatt
     1501 aacttctaca ctttgactga taattggggc cgttgccata aaaatcagca ataataccaa
     1561 caatacatca agaaatggaa caatgttgat ttctgattta atgtcattac gttttaaacg
     1621 acgagacata tattcctctt tacaggtgct tagcacctta attgatctga aataaccggc
     1681 acagtgtccc ggtctcgaat aatgcggtta caagcggtct tttttacgaa aaactttaca
     1741 aattttcatt aaaaatcaac cgcttgtaga ttatcttttt gaaaaagcct gacggtgtaa
     1801 taaggtcgtg aactcatcaa tgaaattcac atacccttgc tcaactttac ctaaacgtaa
     1861 attcaaacgg ttatatgcca ttactgcagg aatcgccgca aataaaccga tagccgttgc
     1921 aattaatgct tctgcaatac ccggtgcgac tgattgtaaa gttgcttgct taactgcact
     1981 taatcccata aaggagtgca taattcccca aaccgttccg aataaaccaa tatatgggct
     2041 gattgaaccc actgtgccta aaaacggaat gtagttttct aaactttcca tttcacggtt
     2101 taatgctaag ttcatcgcac gggtagaacc ttgaataatc gattctggcg catctgggtt
     2161 cgcttgttgt aaacgggtaa attctttaaa cccaacataa aagatttgct ctgaaccatt
     2221 taatgcatca cgacgattct ctaaaccttg gtgtaaacgg tttagatctt cccctgacca
     2281 gaatcgatct tcaaaagcaa gggaatcttt tttggcttta gttaaaattt tgctgcgctg
     2341 aataatcacc gcccaagaca aaattgagaa tacaataagg atcagcatca cgatctttac
     2401 cacaatactt gcttctaaaa ataacgaaag aagactaaat tcagtttgca ttaataaaac
     2461 tccgaatagt ttttaaatag aaaaataact tttttgtaat gcttgacgaa tatcatcagg
     2521 aattgcaacc ggcttcattt tgattaaatc aacacaagcg accgtaacat ttgcctcact
     2581 cagacaatcg ccatctttcc acaatcgttg tttaaagata agggttgctt tcttcaattc
     2641 tgagagtgtt gtttccacat ttaataaatc atctaaacga gcagggagct tataatctat
     2701 ttcaatcctc ttaacaacaa aagcaaggga ttggctaaaa agttcctgct gtgagaaatg
     2761 ctgctgtctc aaaaattccg ttcttgcccg ctcaaaaaaa ttcagatagt tggaatgata
     2821 aactactccg ccggcatcag tatcttcata ataaacacga atcgggaaat ccataagcat
     2881 tatttctctc attcaaaatg aaaaaacgcc caaaattt
//
LOCUS       DANLXS010000243         2907 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.249, whole genome shotgun sequence.
ACCESSION   DANLXS010000243 DANLXS010000000
VERSION     DANLXS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.249"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            68..2107
                     /gene="malQ"
                     /locus_tag="PXD71_002513"
     CDS             68..2107
                     /gene="malQ"
                     /locus_tag="PXD71_002513"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL6292573.1"
                     /translation="MSNKQNTAHYFYSPNIKEYASPSAFEKIERLLNTDNDLQKNNQI
                     LPLVKVITQPNAEQQAVEISLNFTNVAKWQIVLENGEVRNGEIKAEQSVLTLPADLPL
                     GYHQLSISTENDEESCSLIVTPPKAFQPKPLEERKKLWGAFLQLYTLRSDNNWGIGDF
                     GDLKQFLANISDYGADFVGLNPIHALFPADPDAASPYSPSFRLWQNIIYIDVSAVEAF
                     QQSEQAQQWFHTKETQQQLAELRAKDFVDYSQVAQLKLKALQFAFEHFSQSSQQQSQT
                     AFDAFIEQAGEPLKIQATFDALHHWISSEFGEKGGWTNWAEEYQSYYSPKVAEFQISQ
                     ATQIRFYMWLQFVANQQLAECNRFAQSLSMPIGFYRDLAVGVTQSGAETWADKALFVQ
                     EASIGAPPDMLAPNGQSWGLSPMHPHILQSRGYQPFIDLVRANMKHCGALRIDHILGF
                     ARMWWVANGESAKEGIYVRYPLEDLLSILALESQRHQCLVIAEALGIVPEGILDSLEQ
                     KGIFAYNVFYFEKAENAFKPLENYPYQAMTTLSTHDLPTIQGYWKGYDFELGEKFDTY
                     PSKAVLRKLKEERSYDRLNIRNAVEKVIALEPNEVGVTIKFTHQLQRYVAHTHSALFG
                     TQPEDWLNMLEPVNIPGTSTEYPNWRRKLSATTEQIFANKDIQQLLKDINEIRKG"
     gene            complement(2175..2906)
                     /locus_tag="PXD71_002514"
     CDS             complement(2175..2906)
                     /locus_tag="PXD71_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDL6292574.1"
                     /translation="MGCSSFSTKLKQEFFSGWTTFEASWLIVFLAIQIGIFIYQPDTW
                     IATIAAITGILCVVFVGKGKISNYLFGLISVSLYAYISYTFQLYGEMMLNLLVYVPVQ
                     FIGFYFWRKNMTSENTVNNAGVEEVIAKALTAKQWVIVAITTIIGTFLYIELLKYLGS
                     ALAILDGATVVISIVAQILMVLRYREQWALWIIVNIMTISLWTAMYFQNGETSLPLLV
                     MYVMYLCNSIYGYYNWIKLHRKHQQ"
BASE COUNT          931 a          616 c          571 g          789 t
ORIGIN      
        1 cttcttactc atattaatct tcacaagatc gttattttaa caaatacagt aaaggaaaaa
       61 agtcataatg tcaaacaaac aaaatacagc tcactatttt tactccccaa atattaaaga
      121 gtatgccagc ccctctgctt ttgagaaaat agagcggctt ttaaataccg ataacgattt
      181 gcaaaaaaat aatcaaattc taccgcttgt taaagtgatt actcagccaa atgccgagca
      241 acaagcggtc gaaatttcgc tgaattttac aaatgtagca aagtggcaaa ttgtgcttga
      301 aaatggtgag gttcggaacg gtgaaattaa ggcagagcaa tcagttctaa ccctgcctgc
      361 cgatttgcct ttaggctatc atcagctttc tatttcaact gaaaatgatg aagaatcttg
      421 ctcgctgatt gtaacaccgc cgaaagcttt ccagcctaag ccattggaag aaaggaaaaa
      481 gctctggggt gcatttttac agctttatac tttgcgttcc gataacaact ggggaattgg
      541 cgattttggc gatctcaaac aattccttgc caacatttct gattatggtg cagattttgt
      601 gggcttaaac cctattcacg ccctctttcc ggcagatcct gatgcggcaa gcccttacag
      661 cccgtccttt cgactttggc aaaatattat ttatattgat gtttcagcag tagaagcctt
      721 tcaacaatct gaacaggctc aacaatggtt tcacaccaaa gaaactcaac agcaacttgc
      781 cgaattgcga gccaaagatt ttgtcgatta cagccaagta gcgcaactga aattaaaggc
      841 tctgcagttt gcttttgagc atttttcaca gtcatctcaa caacaatcgc aaacagcgtt
      901 cgatgccttt attgagcaag caggcgagcc attaaaaatc caagcaacgt tcgatgcttt
      961 gcaccactgg atttcatctg aatttggcga aaaaggcggc tggacaaact gggcggagga
     1021 atatcaaagt tactattcgc ctaaagtagc tgaatttcaa atatcgcagg caacgcagat
     1081 acgtttttat atgtggttgc aatttgtggc aaaccagcaa ttagcagaat gcaaccggtt
     1141 tgctcaatca ctttcaatgc cgattggttt ttaccgcgat ttagccgttg gtgttactca
     1201 aagcggagcg gaaacttggg cggataaagc cctatttgtg caagaggcgt caatcggtgc
     1261 accacctgat atgcttgccc cgaacggaca gagctgggga ctttcgccaa tgcacccgca
     1321 tattctacaa agccgaggtt atcagccgtt tattgattta gtgcgtgcta atatgaaaca
     1381 ttgtggggca ttgcgaatcg accatattct cggcttcgcc cgtatgtggt gggtggcgaa
     1441 cggtgaatcg gcaaaagaag ggatttatgt ccgttatccg ctcgaagatt tgctctctat
     1501 tttggcatta gaaagccaac gtcaccaatg tttagtgatt gcagaggcac ttggcattgt
     1561 gcctgaaggc attttggact ctctcgagca aaagggcatt tttgcctaca atgtttttta
     1621 ttttgaaaag gcagaaaacg cgtttaaacc gctggaaaat tatccttacc aagcgatgac
     1681 aactctaagc acccacgatt taccgactat tcaaggctat tggaaaggct atgattttga
     1741 gctaggcgaa aaatttgaca cttacccaag caaagccgta ttgagaaagc tgaaagaaga
     1801 gcgttcgtac gaccgcttga atatccgtaa tgcggtggaa aaagtgatag cactcgagcc
     1861 aaatgaagtg ggagtaacaa taaaatttac ccaccaatta caacgctatg tggctcacac
     1921 tcacagtgca ttattcggta cacagcccga agattggctc aatatgttag agccggtgaa
     1981 cattccggga accagcaccg aataccctaa ctggagaaga aaattatccg caaccaccga
     2041 gcagattttt gcaaataaag atattcaaca actgctaaaa gatataaacg aaattcgtaa
     2101 aggataataa caagcggtca tttttaggta aatttttaca aaaaatgacc gcttgtaatt
     2161 tgtatttatt cgctctattg ctgatgtttt ctgtgaagtt taatccagtt gtaataaccg
     2221 taaatagagt tacataaata catcacatac atcaccaaca gcggcaagct ggtttcgccg
     2281 ttttggaagt acattgccgt ccataaagaa atggtcataa tattcacgat aatccacaat
     2341 gcccattgct cacggtagcg aagtaccatc aaaatttgtg ccacaatcga aataaccact
     2401 gttgcaccat ctaatatagc taacgcactg cctaaatatt taagcaattc aatataaaga
     2461 aatgtgccga taatggtggt aatggcgaca atcacccact gttttgcggt taaagcttta
     2521 gcaattacct cttccacacc agcattattt accgtgtttt cgctggtcat atttttacgc
     2581 cagaaataga aaccgataaa ctgcaccggc acatatacaa gtaaattcag catcatttca
     2641 ccatataact gaaaggtata agagatataa gcataaagcg aaacggaaat taagccgaat
     2701 agatagttac tgatttttcc tttaccgaca aataccacgc ataaaatacc tgttattgcc
     2761 gcaatagttg caatccaagt atcgggttga taaataaaaa taccgatttg aatcgctaaa
     2821 aaaacaatca accaacttgc ctcaaaagta gtccaaccac taaaaaactc ttgttttaac
     2881 ttagttgaaa aagatgaaca gcccata
//
LOCUS       DANLXS010000244         2827 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.250, whole genome shotgun sequence.
ACCESSION   DANLXS010000244 DANLXS010000000
VERSION     DANLXS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.250"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..463
                     /locus_tag="PXD71_002515"
     CDS             <1..463
                     /locus_tag="PXD71_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292575.1"
                     /translation="GDRNYQLYIDLATGELVSKLGKGLTTREFDAAVKAFENAFNAKD
                     TAENTSPAESKETEQENKQETTKLVITKPTLKGSEKQIAWADEIINLFIAKIGDTSIP
                     VTATQEQITQAQAMVDNFFAMTNAGKWIEKFKGLNDSSEPKQFWLSVKYGW"
     gene            533..1051
                     /locus_tag="PXD71_002516"
     CDS             533..1051
                     /locus_tag="PXD71_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292576.1"
                     /translation="MTTQVTPQIMRIIGQIVAATYGDDVPTNVQTIILRYPIRGIGFI
                     SSRRELSINNGEIARLMDKIPGDLEDPKDGMPFDCQGAFWLGYYQYCKLSNDVKNYTS
                     KELSIIGESLYGTQWQSNLARDLRLSDARRVREWVAGERKIPFGVWADLTELVKAKKA
                     NLSSILKKLTID"
     gene            complement(1198..1509)
                     /locus_tag="PXD71_002517"
     CDS             complement(1198..1509)
                     /locus_tag="PXD71_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292577.1"
                     /translation="MTKTKHKHPLAEAFYQLGKDVGEINRRLVVVDEQSLKLQELKQT
                     INEQTEQIKTLGTVVNKLIDALQQSQSLNVRSESAVAATENQDHLNDGVQYQGPGRAR
                     A"
     gene            complement(1502..1651)
                     /locus_tag="PXD71_002518"
     CDS             complement(1502..1651)
                     /locus_tag="PXD71_002518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292578.1"
                     /translation="MDNILITIHPTETIDITIDKLVVIHIGEEYGDTPDFLTIYQQAK
                     ELNDD"
     gene            complement(1885..2178)
                     /locus_tag="PXD71_002519"
     CDS             complement(1885..2178)
                     /locus_tag="PXD71_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292579.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGEESDSEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYNHLKETGQIVV"
     gene            complement(2301..>2827)
                     /locus_tag="PXD71_002520"
     CDS             complement(2301..>2827)
                     /locus_tag="PXD71_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292580.1"
                     /translation="AAISANLGKVTAGTITGTTITGNTISGGSINGTTISGTTISGGT
                     IKGARLEGATGKFTGELEVSQLIGGGVIEQVTGKLKYSHRNRIYEGQEHGENSSSPIY
                     RHEYIYKATVTINAAPTRRYIEIIGSESFILEANQSITKTIEKSLNRNASGSSLDFIV
                     LAYSISTAKYISIS"
BASE COUNT          847 a          541 c          562 g          877 t
ORIGIN      
        1 aggtgatcgg aattatcagt tgtacattga cttagccaca ggtgagttag tgagcaaact
       61 tggcaaagga ttaacaacaa gagagttcga cgctgccgta aaagcatttg agaacgcttt
      121 taatgctaaa gacaccgctg aaaatacatc gccagcagaa agcaaagaaa cagagcaaga
      181 gaacaaacaa gaaacaacta agcttgtaat cacaaaacca acattaaaag gctctgaaaa
      241 acaaatcgct tgggcagacg aaatcatcaa tctttttatt gcgaagattg gtgatacctc
      301 tatcccagta acagcaacgc aagaacaaat tactcaagct caagcaatgg tagataattt
      361 ttttgcaatg acaaacgctg gaaagtggat cgaaaaattt aaaggtctaa atgatagtag
      421 cgaaccaaag caattttggt tatccgtaaa atatggatgg tagattgaga taacaaataa
      481 gccgtaagag caaaggctta cggctaaaac aaacagataa ctggagatta ttatgactac
      541 acaagtaaca ccacaaatta tgcgcattat cgggcaaatt gtcgctgcta cttatggtga
      601 cgatgtgcca acaaacgtac agacaataat cttgagatac cctataagag ggatagggtt
      661 tatctctagt cgcagagagc tatctattaa caatggagag atagcaagat taatggataa
      721 aataccaggc gacctcgagg accctaaaga cgggatgcct tttgattgtc agggggcgtt
      781 ttggcttgga tactatcaat actgcaaatt aagtaacgat gtaaaaaatt acacatcaaa
      841 agagctatcc atcattggcg agtcattgta tggcactcaa tggcagtcta atctagcgag
      901 agatttgaga ttatctgatg ctcgccgtgt ccgcgaatgg gtggctggtg aacgtaaaat
      961 ccctttcggg gtttgggctg atttgaccga attggttaaa gccaaaaaag ctaacttatc
     1021 atcaattctt aaaaaattaa caatagatta gcactgattt taaagcccct ctcggggctt
     1081 aattatagaa aataaaatca caatggctaa ttttgccaaa tttgcctgtt aaaacgtgcg
     1141 aaaaattaca aaaaacggct agagctatag ccgttttgag tgcagatgtt aacagagtta
     1201 agctctagca cgtcctgggc cttgatactg cacaccatca tttaaatgat cttgattttc
     1261 ggttgctgct acagcagatt ctgatcttac atttaaactt tgtgactgtt gtaatgcatc
     1321 aataagttta tttaccactg ttccgagtgt tttaatttgc tcggtttgct cattaatagt
     1381 ctgctttaac tcttgcagtt ttaatgattg ctcatccaca accactaatc gacggttaat
     1441 ctctcctacg tctttaccta attgataaaa tgcttctgca agtgggtgtt tgtgtttagt
     1501 tttagtcatc gttaagctcc tttgcttgtt ggtatatagt taaaaagtca ggtgtgtcac
     1561 catactcctc acctatatgg ataactacca gtttgtctat ggtgatatca atagtttcgg
     1621 ttggatgtat agtgattaag atattgtcca tattcccgcc ttaaatttgg gtttggtcag
     1681 ggataagttg gatcgtgccg tagtactgcc attttgggtg gatagctcaa tttgaggctc
     1741 tgtgctgccc tctactatgg cgtgtaaatc cagcctaata ccctctaaat ttacaggctc
     1801 gccatttgtt tgcctaaaaa aaaaggcgat aacttacgtc atcgcctctg aaaaacttaa
     1861 atttatgctt ttgcatttag acccctacac tacaatctga ccggtttctt tgaggtggtt
     1921 gtagatacgt cctaacataa tctgcgttgg cgttttgcca atatcctctt ttgttagcgg
     1981 tgcatcagta atcgctttgg ctgattgagc atcaatatat ttgtactcac ttgtgatgat
     2041 tacaaagttt gttacctcgc tatcgctctc ctcacctgtg ccaacgacat atttagcatt
     2101 gagtgagccg tcgtcttgag ttgagtagct tgcgatggtg gaatacattg ggtttaagat
     2161 tttgttaaaa gttgtcataa tttttccttt tatcgatagt tgaaataaaa acaatcgcct
     2221 gaattgacct gcaagcggtt ggttttggat atttaaaaaa gctgtggcta tccgcactca
     2281 gtactacatt gaggcgataa ctaactgata gagatatatt tagccgtcga aatggaatag
     2341 gctaaaacaa taaaatctaa agagctacca cttgcatttc tatttaatga tttctctatt
     2401 gtttttgtga ttgattgatt tgcttctaaa atgaatgact cacttccgat gatttcaata
     2461 tacctccttg tcggcgctgc atttatggtg acagttgctt tatagatata ttcgtgtcta
     2521 taaattggag aggaggagtt ttctccatgt tcttgcccct cataaatgcg attacgatgt
     2581 gaatatttta atttgcctgt aacctgctct ataacacctc cccctattaa ttgagatact
     2641 tcaagttcgc cggtaaattt gccagtagcc ccctccaatc ttgcaccctt tattgtgccg
     2701 ccagagatag ttgtaccgct aattgttgta ccattaatac ttccgccact aattgtatta
     2761 ccagtaattg tcgtacctgt aattgtacca gcagttacct taccaagatt tgccgatatt
     2821 gccgcta
//
LOCUS       DANLXS010000245         2813 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.251, whole genome shotgun sequence.
ACCESSION   DANLXS010000245 DANLXS010000000
VERSION     DANLXS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.251"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            220..468
                     /locus_tag="PXD71_002521"
     CDS             220..468
                     /locus_tag="PXD71_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292581.1"
                     /translation="MISGEKMTWESLGASAIGGAIGGGIGATMKAGAVTASTLSALGG
                     GMGEGLYKSTSQGIKNSIGIKASCNQSGSDYGDGCDYQ"
     gene            480..683
                     /locus_tag="PXD71_002522"
     CDS             480..683
                     /locus_tag="PXD71_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292582.1"
                     /translation="MKIQFPISYQEFRENYFEKKPLLMKGAISQKDLLSWKSINEILP
                     RCDLISEDAIKVMHKGKRAHKKD"
     gene            722..1364
                     /locus_tag="PXD71_002523"
                     /pseudo
     CDS             722..1364
                     /locus_tag="PXD71_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1504..2574
                     /locus_tag="PXD71_002524"
     CDS             1504..2574
                     /locus_tag="PXD71_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292583.1"
                     /translation="MKFNKKLILTFAATLVLSACGGSGSGGSSSTPNHPKPVLVPKTQ
                     NNLQAQNVPQAQNASQAQNAPQAQNAPQAQNAPQVENAPQAQNAPQVENAPQAEVTPP
                     VPQPQSQKIDGSFDKIGSVKLNKEAQTLELSRFTLVDKLGTPPKFDKVSGKKIIEEKD
                     FLVLNLSDINAEQLSGDFLIRRSDDLFYGYYHDTNGKNLVDAADKFSQYFVVYDEKRV
                     NDNISDKLTATYRKKEGFVYGSNPHTKEFAARISKLGDVEIKFENGQAQGSIKDEKDG
                     NAEIFTIKGDTKQLEITPTESNRIIIAILDQNQKSYTPGMEKAIMETKFIDSKAGNSD
                     QKYLIGEAKSDNWQAIMVSEKK"
BASE COUNT          968 a          468 c          573 g          804 t
ORIGIN      
        1 ttgtgtctat tcttcttgta aagaagattg taaaaaataa aaatgtacaa gatgttattg
       61 aggctatagt tctaatagta tttgttgtaa ttataaacat catagaggaa tatctcttat
      121 gaaagagtta aaaattgagg attgccaatt tgtttcggca ggcttgcact tgttattgta
      181 ggagctgttg ctggagctgc atattatgtt gggaatggta tgatttctgg ggaaaaaatg
      241 acttgggaga gtttgggagc atcagctata ggtggggcta ttggtggcgg tattggagca
      301 acaatgaaag ctggtgcggt gacagcgtca accttatctg ctttaggtgg tggtatgggg
      361 gagggcttgt ataaaagtac ttctcaaggt atcaaaaata gtattggtat aaaggcgagt
      421 tgcaatcaat caggttcgga ttatggtgat ggatgtgatt atcaataaga gagtatatta
      481 tgaaaattca atttccaatt tcttatcaag aatttcgtga aaactatttt gagaaaaaac
      541 cacttttaat gaaaggagca atatctcaga aagatttact ttcttggaag tcaataaatg
      601 aaatattacc tcgctgtgat ttaatttctg aggatgcaat taaggtaatg cataaaggga
      661 aaagggctca taaaaaagat taggggctgt agtagattag catcactgct atactacaaa
      721 aatgaagata acccattgta aattaaagaa atctttgcag aaaaagcttc ttgaattttt
      781 tgttgctgaa gtgaccgcta gaaccgctgc tgatttatta ggtattcaac ccaatacggc
      841 agccctgttt taccacaaaa ttaggctggt cattgaacac catttagccc ttgaagctca
      901 tgaaattttt gagggaaaaa ttgaggtaga tgaaagctat tttggtggtc atcgcaaagg
      961 aaaacgaggg cgaggtgcgg ctgggaaagt cgctgtattc ggtttgttga agcgtcaagg
     1021 aaaggtgttt acaaaatacc aaatcagaaa cgctaatgcc ggttattaca aggaaaatca
     1081 agcctgacag ctgggtttat acggatacct atcgtagcta tgatgccctt gatgtgagcg
     1141 aatttcatca cgagcgaatc aaccattccg aactgtttgc ggagaaacaa aaccatatca
     1201 atggcattga aaatttttgg aatcaagcga agcgagtact ccgaaaatat aacggaatta
     1261 accgaaaaaa tttcccttta ttcttgaagg aatgtgaatt tcggtttaac tttggtacac
     1321 caaaagaaca gctcaaaacg ttgcgaaaat ggtgtgagat ttagggctaa tctactacag
     1381 ccccaaaaat tttcataagg gaaacgttta cgtaaaactc ctcagaccac tcattcttat
     1441 tttatataaa aatatgtgat agacttctcg cagtttcgtt ttatatattt aaggaataac
     1501 taagtgaaat tcaataaaaa attaatttta acatttgctg caaccttagt tttaagtgct
     1561 tgcggaggaa gcggtagcgg aggttcgtct tcaacaccga atcaccccaa accagtacta
     1621 gtaccaaaaa cacaaaataa tcttcaagca caaaatgttc ctcaggcaca aaatgcctct
     1681 caggcacaaa atgcccctca ggcacaaaat gctcctcagg cacaaaatgc tcctcaggtg
     1741 gaaaatgctc ctcaggcaca aaatgctcct caggtagaaa atgctcctca agcagaggtt
     1801 actccgcctg taccacagcc acaatcacaa aaaattgacg gttcttttga taaaattggt
     1861 tcagtaaaac tcaataaaga ggctcaaact cttgagctta gtagattcac tttggtggat
     1921 aaattaggca caccaccgaa gtttgataaa gtaagcggta aaaaaattat tgaagaaaaa
     1981 gattttctcg tattaaattt gtctgatatt aatgctgaac aactctctgg cgattttctt
     2041 attcgccgta gcgatgatct attctatggc tactatcacg atacaaatgg caaaaatctt
     2101 gtcgatgctg ccgataaatt cagtcaatat tttgtcgtgt atgatgagaa acgggtaaat
     2161 gataatatct ctgataaatt aacagcaact taccgtaaaa aagaaggctt tgtatatggt
     2221 tcaaatccac atactaaaga atttgccgca cggatcagca aattggggga tgtagaaatt
     2281 aaatttgaaa atggtcaagc tcaaggaagt ataaaagacg aaaaagatgg aaatgctgag
     2341 atctttacta ttaaaggtga tacaaaacag ttagagatta ccccaacgga aagtaaccga
     2401 atcattatag caattttaga ccaaaatcaa aaaagctata ctccaggaat ggaaaaagca
     2461 attatggaaa ctaagtttat tgattcaaag gctggtaatt ccgaccaaaa atacttaatc
     2521 ggtgaagcaa aaagcgataa ctggcaagca ataatggtta gcgagaaaaa ataaagttat
     2581 cttttgctaa aaactgaaat aaaaaggctg agtccgggta atatcggcct cagtctttta
     2641 aattgtagaa aatcatctgt agaagatcaa acctaatctg acagtccccc gttttaaatt
     2701 accgtgtctg tcagattaat ttgagcttaa attcttttcc acccaaatcc gttttccatc
     2761 aagtaaggtt gccatcggtg ttctgccata gcacattttt ccttgatgtg ttc
//
LOCUS       DANLXS010000246         2793 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.252, whole genome shotgun sequence.
ACCESSION   DANLXS010000246 DANLXS010000000
VERSION     DANLXS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.252"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>2793
                     /locus_tag="PXD71_002525"
     CDS             <1..>2793
                     /locus_tag="PXD71_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292584.1"
                     /translation="WEIYDLAPILSKMLGVEISFDKWALYDVARYCDQLVPDGMGGME
                     PRFTCNVWLTEVKTAYDLLNDFCSVFRAIPIWTGTEVSVIIDRPRDPVWTYTNANVVG
                     GFERSYSARKSRHNAVQVTYSDKTNGYESAIEYVSDDEEIKKHGLNLSQITAFGCTSR
                     GQAHRTGKWILETEKREKETITFTVGREGLMHLPGDIIRVADSHYAGTEIGGRVLAIN
                     GRKVTLDREISIDNASYFTYINGEATHSSKIQSVNGKEITLDSTPTGLETYGVWSLST
                     KQISSGLYRSISIVENADGTNTITALQHEPQKEAIVDNSAHFVETARTLYKAPQINAV
                     EVSTGYDGKLYITGDISSGDGKLTYDIKITKDGNLYQYKKGLSDPNIELSDLPNGDYT
                     VIIYGKNAKGQIVTEKTQTFTIDRPPAPTGVVVTGGLGQITLEWDWVNEVTQTEIFAA
                     ETDNFALAKKIAKVTARTYAHTLKGNKVVRYYWLRHTRGINVGPFYQQQGVRGQTAVD
                     LDARLTELNTQLSRNIVNEVFDVAAPARGLELVKTVANLTDKGTKLASSQVYNQADGK
                     LYTWNGTAYSATVAAEDVTGKLSKSKIDTSLISQLTGADNTANLARRLAETAQSNINQ
                     EITNRQDAVTAEANNRTKAIQAESANLTKKIQAEATARGAAVTQLQNVDAQQAQLISA
                     VTAKADNALSGLEEEKTARANADKAESQAREALTARMGAAESNIVTIQRTVANNAQSI
                     SEVSQNLNAKIDNINIGGRNLLRDSEFNAYNKWGSPQIEFAENANRRTIKVTSTGTNG
                     PVGIVSSNRHSTSYFQQGETYTLSLFARGSKALDYLYLMRQDGNNVRLPVINVASETE
                     FNHYKLTFKAPFTTQQGYVLIGFRQTSTEQFVEFHSVKLEKGNVATDWTPAPEDVESA
                     VSAVSADLTSYKQTQA"
BASE COUNT          896 a          578 c          623 g          696 t
ORIGIN      
        1 cttgggaaat ttacgattta gctccgattt taagcaaaat gcttggggtt gaaatcagtt
       61 ttgacaaatg ggcgttgtat gatgttgctc gctattgcga tcaacttgtg ccggacggta
      121 tgggtggaat ggagcctcgt ttcacctgta acgtatggct cacggaagtt aaaactgcct
      181 atgacttgtt gaatgatttc tgctcggtat tccgtgcgat tccgatttgg accggaacag
      241 aagtatcggt cattatcgac cgtccgcgtg atccggtttg gacttacaca aatgctaatg
      301 tagttggtgg atttgaacga tcctactctg ctcgcaaatc acgccataat gcagtgcaag
      361 ttacttattc ggataaaaca aatggctatg aaagtgcgat tgaatatgtc tctgatgacg
      421 aggaaatcaa aaagcacggc ttaaatttaa gtcaaattac tgctttcggt tgtacctctc
      481 gggggcaagc acaccgtacc ggaaaatgga ttttggaaac cgaaaaacgg gaaaaggaaa
      541 ccattacttt taccgtggga cgtgagggct taatgcactt gccgggcgat attattcgtg
      601 tggcagacag ccattatgcc ggtacggaaa ttggcggacg tgtactggcg attaatgggc
      661 gaaaagtcac gttagatcgt gaaattagca ttgataacgc cagttatttt acctatatca
      721 acggtgaggc aacgcactct agcaaaatcc aatcggtaaa cggcaaagaa attacgctgg
      781 atagtacacc aaccggctta gaaacctacg gtgtttggtc gttatcgact aaacaaatca
      841 gctctggatt atatcgctca atctcgattg tcgaaaatgc ggacggcaca aacactatca
      901 cagcgttaca acacgaaccg cagaaagagg caattgttga taactccgct cactttgtgg
      961 aaacggctag aacactctac aaagctccac aaattaacgc agtggaagtc tcgaccggtt
     1021 atgacggtaa gctctacatt accggtgata ttagctccgg tgatggcaag ctaacctatg
     1081 atattaaaat taccaaagat ggcaatttat atcaatacaa aaaaggctta agcgatccga
     1141 acattgaact aagtgattta cctaacggcg attataccgt cattatttac ggcaaaaacg
     1201 ccaaagggca aatcgtcacc gaaaaaacac aaacctttac tattgatcga cctccagctc
     1261 cgaccggtgt agtagtaaca ggtggattag ggcaaattac cctcgaatgg gattgggtaa
     1321 atgaggttac gcaaaccgag attttcgcgg ccgaaaccga taatttcgcc cttgcaaaaa
     1381 aaatcgcaaa agtgaccgct agaacctacg ctcacacact aaaaggtaac aaggttgtac
     1441 gctattattg gctacgtcat accagaggca ttaatgtcgg accgttttat caacaacagg
     1501 gggtaagagg acaaacagcg gttgatttgg atgctcgatt aaccgaatta aacactcagt
     1561 tatcacgcaa tattgttaat gaggtttttg atgttgctgc accggcaaga ggtttagaat
     1621 tagtgaaaac agtcgctaat ttgaccgaca aaggtactaa actggcgtca tctcaagttt
     1681 acaatcaggc tgacggcaag ctctatactt ggaacggtac agcatactca gctacagtgg
     1741 ctgcggaaga tgttaccggc aaattatcaa aatctaaaat tgatacgtcc cttatttcgc
     1801 aattaacagg tgcagataac accgcaaatt tggcaagaag attagcggaa acagctcaaa
     1861 gcaacattaa ccaagagatt actaaccgac aagatgccgt tactgccgaa gcaaataacc
     1921 gtactaaagc cattcaagcc gaaagtgcga atttaacgaa aaaaattcaa gctgaagcaa
     1981 ccgctcgtgg ggctgctgtt acacagttac aaaatgttga tgctcaacaa gcacagttga
     2041 tttctgctgt cacagcaaaa gctgataatg ctctttcagg cttggaagaa gaaaaaacag
     2101 cacgagcaaa cgccgataaa gcggagtctc aagcacgtga agcattaaca gcaagaatgg
     2161 gagcagctga aagcaatatc gtaactattc aacgtacggt agcaaataat gcccaaagca
     2221 ttagtgaagt cagtcagaat ttaaatgcta agattgataa tattaatatc ggcggtcgga
     2281 atttattaag agacagcgag ttcaatgcgt acaataaatg gggtagccca caaattgaat
     2341 ttgctgaaaa tgcaaatcgc cgaactatca aagtaacctc aacaggtact aacggtcctg
     2401 ttggtattgt ttcatctaat cgccattcca catcttattt ccaacaagga gaaacttata
     2461 cactctcatt atttgctcga ggaagtaagg cactagacta tctctatcta atgcgtcaag
     2521 atgggaataa cgttagatta ccagtgatta acgttgcctc tgaaactgag tttaatcatt
     2581 acaaactcac gttcaaggct ccttttacca cgcaacaagg ttatgtctta atcggttttc
     2641 ggcaaaccag cacagagcag tttgttgagt ttcacagcgt caaacttgaa aaaggcaacg
     2701 tagccacaga ctggacacca gcaccggagg atgtggagag tgcggttagt gctgtttcgg
     2761 ctgatttaac gagttataaa caaactcaag cgg
//
LOCUS       DANLXS010000247         2690 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.253, whole genome shotgun sequence.
ACCESSION   DANLXS010000247 DANLXS010000000
VERSION     DANLXS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.253"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..170
                     /locus_tag="PXD71_002526"
     CDS             <1..170
                     /locus_tag="PXD71_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292585.1"
                     /translation="KGYDEFLAEKIRLGLEDMQSGNGLSLDESKARTKQLIERKAREL
                     ANFEQENIIYG"
     gene            163..450
                     /locus_tag="PXD71_002527"
     CDS             163..450
                     /locus_tag="PXD71_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6292586.1"
                     /translation="MAKTLRISSRAQNDIEEILASVIEYTGFESSGIRLQEDIYQKFE
                     TIAYMPSAAGRLREDGTREAFTRRYRIVYTELENEVWIITIIHSSRLYPRI"
     gene            complement(576..2678)
                     /locus_tag="PXD71_002528"
     CDS             complement(576..2678)
                     /locus_tag="PXD71_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL6292587.1"
                     /translation="MKSFKLLPVSAAVLSVLAANAVAQTASTDAVVLDTVTVTDNQGL
                     KVQSNVVTTKKKDESTQTDLRGLLKDEPAISLGGGNGTSQYLYIRGMGQNSIDVKVDN
                     TYSDSQILYHQGRHMLDPALVKIVSVQKGAGSASAGIGQTNGAVIAKTVDALDLLKNS
                     DKNFGAKLGTGFSTNHAHNFNATLYGKGEIFDALVSGNLVRDRDYKGGKDYVNQFGTN
                     RVPYSALDKTSFLAKLGATLGDHRFVLSHSSERNEGQRLVREEFDVAPNGTKVSPYNR
                     LTVERQAPANRRITVQRTNLEWTGKNLGFAQEATANVYQLVQGRWSENETGNGYAGGV
                     DYATKTKIVTHGANVNFDSALHENVLLKYGVNYRHQEAKPHTKFNPLVKNQEKTDTGV
                     YVEAITAPVDKVTLTTGVRYDHFKFKAMDGKERSNGAFSPSVGLIYEPIEHLSLSASH
                     NYATRSPRLYDALTAHGNRKIVSIGSNTKAEKARNTEIGFNYNNGTFSFDGSYFWQDI
                     KDALGGGRDSHNPNNELIMNLGKIKNRGYELNAGYHNGGFTARIGVAHSKPRFYSPTI
                     WEVNRQTGKREEKPLLSTNPEYASAIGRTWTASLSYRFEQPNLEVGVHHRIVEKVKPE
                     DNFFVTGGTLQTNTGTGKDGYNVTDITLNWKPLKDDSVNVNFAVDNVANKKYNAHGQR
                     GQLSARGREFRAGVNYTF"
BASE COUNT          773 a          538 c          505 g          874 t
ORIGIN      
        1 agaaaggcta tgatgaattc ctagcagaaa aaattcgcct tggcttagaa gatatgcaat
       61 caggtaatgg tctttcactc gatgaatcaa aagcacgaac caaacaactg attgaacgta
      121 aagcaagaga actcgcaaac tttgaacaag agaatatcat ttatggctaa aacattacga
      181 attagcagta gagctcaaaa tgatattgaa gaaattttag cgagcgtaat tgaatatact
      241 ggttttgaaa gtagtggaat tcgcttacaa gaagatattt accaaaaatt tgagactatc
      301 gcttatatgc cctctgcggc tggacgttta agagaagatg gcacaagaga agcattcact
      361 aggcgttatc gtattgttta taccgaatta gaaaatgaag tgtggattat taccattatt
      421 catagtagca gactttatcc acgcatttaa ataacaaacg actaaaatag tcggtttttt
      481 atttgcaaaa aattaataaa atatcaccgc ttgtaagctc cataaaaaaa agcccgattg
      541 taacatcggg cgttttagtc tgaatttaag tcagattaga atgtgtagtt cacacctgca
      601 cggaattcac gtccacgagc tgataattga ccacgctgac cgtgtgcgtt gtatttttta
      661 ttcgccacgt tatccactgc gaagtttaca ttcacgctat catctttaag tggtttccag
      721 tttaaggtga tatcagttac attgtagccg tctttgcctg taccggtatt tgtttgtaat
      781 gttccgcccg taacgaagaa attatcttca ggtttcactt tctcaacaat acggtggtgt
      841 acgccaacct ctaaatttgg ttgttcaaag cgatagctta aagaagctgt ccaagtacga
      901 ccgatagctg aggcgtattc tgggttagta cttaataatg gtttttcttc ccgcttacca
      961 gtttgtctgt ttacttccca aatagttggt gagtagaaac gcggtttgct gtgtgcaaca
     1021 ccaatacgag cagtaaaacc accattatgg taacctgcat ttaactcata accacggttt
     1081 ttaattttac ctaggttcat aattaactca ttattaggat tatggctatc acgaccacca
     1141 cctaatgcat cttttatatc ttgccagaaa tagctaccat caaaactgaa tgtaccattg
     1201 ttatagttga aaccgatttc agtattacga gctttctccg ctttagtatt gctaccaata
     1261 ctaacgattt ttctatttcc gtgagccgtt agtgcatcgt ataaacgagg gctacgagtt
     1321 gcatagttgt ggcttgcact taagcttaaa tgttcaattg gctcatagat aagcccaacg
     1381 cttgggctga atgcaccatt actacgctct ttaccatcca ttgctttgaa tttgaagtgg
     1441 tcgtaacgta caccggtagt taaagttact ttatctactg gagcagtaat tgcttcaaca
     1501 tacacgccgg tatcggtttt ctcttggttt ttcacaagag ggttaaattt agtgtgtggt
     1561 ttagcttctt ggtggcgata gttcacaccg tattttaata atacattttc gtgaagggca
     1621 gaatcaaagt ttacgtttgc accgtgggtt actattttag ttttggttgc ataatctaca
     1681 ccaccagcat aaccattacc tgtttcattc tcagaccaac gaccttgtac taactgatac
     1741 acgttagctg ttgcttcttg agcaaaacct aagtttttac cagtccattc taagttagtt
     1801 ctttgaaccg taattctacg gttagctgga gcttggcgtt ctacagttaa gcggttgtac
     1861 ggagaaacct ttgtgccgtt aggtgctaca tcaaattctt cacgcactaa acgctgacct
     1921 tcatttcgct cacttgaatg gcttaataca aaacggtgat ctcccaatgt tgcacctaat
     1981 ttagctaaaa agctggtttt atctagtgca ctatacggaa cacggtttgt gccgaattgg
     2041 tttacgtaat ctttaccgcc tttatagtcg cgatctctca caagattacc tgagactaaa
     2101 gcatcaaaga tttcgccttt accatataag gttgcattga aattgtgagc gtggtttgtg
     2161 ctgaagccag tacctaattt tgcaccgaag tttttatcgc tgttttttaa taaatctaat
     2221 gcatcaacgg ttttagcaat tactgcacca ttagtttgac caataccggc tgaagcactg
     2281 cctgcacctt tttgtacaga aacaattttc accaatgccg gatctaacat atgacgacct
     2341 tggtgataca aaatttggct atctgagtaa gtgttatcta cttttacatc aattgagttt
     2401 tgacccatac cacgaatata gagatattgt gatgtaccgt taccaccacc taagctaatt
     2461 gccggctcat cttttaataa accacgcaaa tcggtttggg tgctttcatc ttttttctta
     2521 gttgttacca cattgctttg tacttttaaa ccttgattat ctgttacggt aacagtatct
     2581 aatactactg catctgttga agcagtttga gccactgcat tagcagcaag tacgctcaat
     2641 accgcagcag aaacagggag caatttgaag cttttcattt tatttatatt
//
LOCUS       DANLXS010000248         2632 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.254, whole genome shotgun sequence.
ACCESSION   DANLXS010000248 DANLXS010000000
VERSION     DANLXS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.254"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            14..772
                     /locus_tag="PXD71_002529"
     CDS             14..772
                     /locus_tag="PXD71_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL6292588.1"
                     /translation="MIPLERQREILRLINNNGVASINFLAEQLKVSHMTIRRDIQKLE
                     EQGKVSLVSGGVQSLERLIMELPHDDKSMLFQEQKSAIGSLASQLIPSNTTIYLDAGT
                     TTLEIAHRIIDRNDLLVVTNDFVIAHYLSNSGKCDLIHIGGSVCKENLSTTGSLAAEF
                     LKNLSIDMAFISTSSWNLKGLTTPNENKIPVKKAILEASKQNILVTDSSKYGKVATFF
                     VCPLTVFDQIICDKGLFENVRDALKGLSIKLDLG"
     gene            complement(912..2303)
                     /gene="ffh"
                     /locus_tag="PXD71_002530"
     CDS             complement(912..2303)
                     /gene="ffh"
                     /locus_tag="PXD71_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL6292589.1"
                     /translation="MFENLSDRLSKTLRNITGKGRLTEDNIKDTLREVRMALLEADVA
                     LPVVRDFINKVKERALGVEVNKSLTPGQEFLKIVQSELETAMGEANEELNLAAQPPAV
                     ILMAGLQGAGKTTSVGKLAKFLKERHKKKVLVVSADVYRPAAIKQLQTLAEALKVDFF
                     PTETSQKPVAIAEAALKHAKLNFFDVLIVDTAGRLHVDGEMMEEIQQIHAVLNPIETL
                     FTVDAMTGQDAANTAKAFNEALPLTGVILTKVDGDARGGAALSIRQITGKPIKFLGVG
                     EKTDALEPFHPDRVASRILGMGDVMSLIEDIQRSVDQEKAEKIAAKFKKGDEFTLEDF
                     REQLIEMKKMGGMMSMLDKLPGAKNLPDHVKSQVDDKMFMKMEAIINSMTLKERANPD
                     IIKGSRRRRIALGSGTQVQDVNKLLKQFDDMQRMMKKMRKGGMAKMMRGVKGMMGGGL
                     GGLGGLGSMFGKR"
BASE COUNT          792 a          552 c          513 g          775 t
ORIGIN      
        1 aataggttaa aaaatgatcc ccttagagag acaacgagaa atactacgac ttattaataa
       61 taatggtgtc gcaagtatta attttctggc agaacagcta aaagtatcac atatgacaat
      121 tcgccgtgat attcaaaagc tggaagagca aggtaaggta tccctagtat ctggtggagt
      181 tcaatctctt gaacgattaa tcatggaatt accacacgat gataaatcaa tgttattcca
      241 agagcagaaa tcagctatag gatctttagc atcacagcta attccctcaa acacaactat
      301 ttatcttgat gctggaacta ctacattaga aatagctcat cggataatcg atagaaatga
      361 cttactagtt gtaacaaacg attttgttat tgctcactat cttagtaatt caggaaaatg
      421 tgatttaatt catataggag gaagtgtctg taaagaaaac ctatcgacaa ctggttcact
      481 tgcagcagaa tttctgaaaa acctatcaat cgatatggca tttatatcta cttcatcctg
      541 gaatttaaaa ggtctaacaa cgccgaacga aaataaaatc cccgttaaaa aagcaatttt
      601 agaggcatcc aagcaaaaca ttttagtgac agactcttca aaatatggaa aagtcgcaac
      661 tttttttgtt tgccctctta ctgtatttga ccagattatt tgtgataaag gattatttga
      721 gaatgtacga gacgcactga aagggctaag tattaaatta gatttaggat aatcttctta
      781 ccaatttttt ctgagaacga caagcaatca aaaagtatta tttttgcaaa taagatatta
      841 atagggaaag ttaacaatcc tagcaacgga ttgttaactg attttcggct ggtactaata
      901 aaataggaat attaacgttt accaaacata ctgcctaagc caccgagacc gcctagacca
      961 ccacccatca tacctttaac accacgcatc atttttgcca ttccgccttt acgcattttt
     1021 ttcatcatac gttgcatatc atcaaactgc ttgagcagct tatttacatc ttgcacctgt
     1081 gtgccggagc caagtgcgat acggcggcgg cgtgagcctt taataatatc cggatttgcc
     1141 cgctctttta aggtcataga attgatgatg gcttccattt tcatgaacat tttatcatca
     1201 acctgacttt taacgtggtc aggcaggttt ttcgcccccg gtagtttatc gagcatcgac
     1261 atcattccac ccattttttt catttcgata agctgctcac ggaaatcttc caaggtaaat
     1321 tcatcgcctt ttttgaattt ggcggcaatt ttttccgctt tttcttgatc aaccgagcgt
     1381 tgaatatctt caattaaaga cattacatcg cccataccta aaatgcggga agccacacgg
     1441 tcagggtgga acggctcaag ggcatcagtt ttttcaccca cacctaagaa tttaatcggt
     1501 ttaccggtga tttggcgaat agaaagtgcc gcaccgcctc gggcatcgcc atcgactttg
     1561 gtaagaataa caccggtaag cggcaacgct tcgttaaagg cttttgcggt gttggcggcg
     1621 tcttgaccgg tcatcgcatc aaccgtaaat agcgtttcaa tcggattcag cacagcgtgg
     1681 atttgttgga tctcttccat catttcgcca tcaacgtgta aacgaccggc agtatccaca
     1741 atcaatacat caaagaaatt aagcttagcg tgcttcagtg ccgcttctgc aatcgctacc
     1801 ggtttttggc tggtttcggt cgggaagaag tccactttta acgcttcagc aagggtctgt
     1861 agctgcttga tagcggcagg gcggtaaaca tcggcagaaa ctaccaacac ctttttcttg
     1921 tggcgttctt tcaggaattt tgccaatttg ccgacagagg tggttttacc cgcaccttgc
     1981 aaacctgcca ttaaaattac cgcaggcggt tgggccgcaa gattcagctc ctcgttggct
     2041 tctcccattg ccgtttctaa ttcgctttgt acgattttta agaactcttg ccccggcgtg
     2101 aggcttttgt ttacctcaac ccctaacgca cgctctttga ccttgttgat gaaatcacgc
     2161 acaacaggga gggcaacatc cgcctccaac aatgccatac gaacttcacg cagggtatct
     2221 ttaatattat cttccgttag gcgacctttg cccgtaatgt tacgcagcgt tttagacagt
     2281 ctgtcagaga ggttttcaaa catatcagct ttctcaattt gcaaaatttt aggaaaaaag
     2341 taccgcttgt gagcggaaag ttgccgccat tatacaaaaa aaagcgttac ggttataggg
     2401 aaataaaaaa acggcatagc gattaaacta tgccgtaaaa tttgacgata ttcgaggaat
     2461 cattatttag ttatattgtg cgatcataat actccgaatt gcgtataaaa gtgaagtgat
     2521 attttcaaat ttatgttaga aatgagaggt aaattacaaa attaaagggg atattattgt
     2581 ttgaaaaaat aagagcgaaa agaaaaccct gatgattgct catcagggct tc
//
LOCUS       DANLXS010000249         2606 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.255, whole genome shotgun sequence.
ACCESSION   DANLXS010000249 DANLXS010000000
VERSION     DANLXS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.255"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            57..2405
                     /locus_tag="PXD71_002531"
     CDS             57..2405
                     /locus_tag="PXD71_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL6292590.1"
                     /translation="MLISAQFSPLVWAENNDVAVLDEVSVVGSTPSISQGSEVTLLKV
                     SDKIIAGKEFKKRSATLGNALASELGVHSNPFGGGASKPIIRGQEGARIRILQNGSDV
                     IDMSNLSPDHAVVADSLLADQVEILRGSSTLLYASSSPVGIVNVVDKRIPTAIPEKGY
                     EVELNSRFDTAAKEKVGALGATFGIGNHIAVRVEGLSRHSDNYRVPGINLGERLNYVP
                     DTYNKSKVGTLGLSFVGERGYIGASYSKRRDNYGLPGHNHKFDFCTGHIYGNKRDKYA
                     YTYLYPHLIGEENIGSNPHFHCGTNHAEDGTHSHDNPFGHAHDHTHKGPWVDLESKRI
                     DVKAELRQPFKGVDKIKASYADADYYHDEKDAGVLATRYHKQLKKDQDYGKPVNIFKN
                     RGKNTRLEVYHAPLGGLTGVWGVQYQTQKSSMNAPKDREVKFPLVENTNKQFSLFGVE
                     QYMWDSVAVELAGRMEKQKIEIEYDRNEIKRLQEHYRISGGKQVEPDLSPYDETAYAY
                     SGTLNWFFHPDYQLSFTASHNERLPTPMELYYHGQHIATNSFEYGNKDLKKEQSNNVE
                     LGLGYQAERIGYKVSVYYNHFKNYIYNENLFRENQLFMRRYNQAKARFYGVEAEASYR
                     FNDQYQATIFGDMVRGWLTNLPPLKINSDYSVFKDYLPENVEKGKDYLLYRADQNTPR
                     TPPMRLGFRFNAEFTPNWSGDLELIRTFTQRRTSQLEYITEGNTMLNVGLSYSNKWKE
                     LDYKISLNGTNLLNQPVYIHTSYHQFVPQMGRNFMLGMEMKF"
BASE COUNT          765 a          524 c          625 g          692 t
ORIGIN      
        1 tattatcctt aattttttta aggagaaaga ttgtgtttaa gaaaacagga ttggcattgc
       61 tgatttcggc tcaatttagc ccgcttgttt gggcggaaaa taacgatgta gcggtgttag
      121 atgaagtttc ggtcgttggc agcactccaa gtatatcgca ggggagtgaa gttaccttgc
      181 taaaagtcag tgataaaatt attgcaggta aggaatttaa aaagcgttcg gcaacgctgg
      241 gtaatgcgtt ggcgtcagaa ttaggcgtgc atagtaaccc gtttggtggc ggagcgagta
      301 agccgattat tcgtgggcag gaaggggcga gaattcgcat tttgcaaaac ggttcagacg
      361 tgattgatat gtcgaaccta tctcctgatc acgccgttgt tgctgattca ctgcttgccg
      421 atcaggtcga aatcttgcgt ggttccagca ccttgcttta tgccagcagt tcgccggtag
      481 gaattgtaaa tgtggtggat aaacgtattc cgaccgctat tcctgaaaag ggatatgagg
      541 ttgaactcaa tagccgtttt gataccgccg ccaaagagaa agtcggcgca ttaggggcaa
      601 cctttggcat cggtaaccac attgcggtgc gagtcgaagg gctgagtcgt catagtgata
      661 actaccgtgt gccggggatc aacttaggcg agcgcttaaa ctatgtgccg gacacttaca
      721 ataaatccaa agtcggcacg ttgggcttat cctttgtcgg tgaacgaggc tatattggtg
      781 cctcatacag caaacggcgg gataattatg gcttgccggg gcataaccat aaatttgatt
      841 tctgtaccgg tcatatctac ggtaataagc gggataaata cgcttatacc tatctctatc
      901 cgcatttgat tggtgaggaa aatattggga gcaatccgca cttccactgt ggaaccaacc
      961 acgccgagga cggcacacac agccacgata acccgttcgg acacgcccac gatcacactc
     1021 acaaaggccc gtgggtggat ttagaatcaa aacggattga tgtgaaagcc gagctacgcc
     1081 agccgtttaa gggcgtagat aaaattaagg caagctatgc tgatgccgat tattaccacg
     1141 atgaaaaaga tgcaggcgtg cttgccaccc gttaccataa acagctgaaa aaagatcagg
     1201 attacggcaa gccggtgaat attttcaaaa atcggggcaa aaatacccgc ttggaggtct
     1261 atcacgcacc gctaggtggc ttaaccggtg tatggggtgt tcagtatcaa acgcaaaaaa
     1321 gcagtatgaa tgcgccgaaa gatcgcgaag tgaaattccc gctggttgaa aataccaata
     1381 aacagttcag cctgtttggg gttgagcagt atatgtggga tagcgttgct gttgagcttg
     1441 ccggacgaat ggaaaagcag aagattgaga ttgagtatga tcgcaatgaa atcaaacggt
     1501 tacaagaaca ctaccgtatc agtggcggca aacaggttga gccagatctt tcaccctatg
     1561 atgaaacggc ttatgcctat tccggcacgc ttaactggtt cttccaccct gactatcagc
     1621 tttcgtttac tgcctcgcac aatgaacgct tgccaacgcc aatggagctg tattatcacg
     1681 ggcagcatat tgctactaat tcgtttgaat acggcaataa agacttgaaa aaagagcagt
     1741 caaataatgt ggagttgggc ttgggctatc aagctgaacg tattggctat aaagtgagtg
     1801 tttattacaa tcactttaaa aactacatct acaatgaaaa tcttttccgt gaaaatcagc
     1861 tatttatgcg ccgttataat caggcaaaag cccgttttta cggggtagaa gcagaggcaa
     1921 gctaccgctt taacgaccaa taccaagcca cgattttcgg cgatatggta cgaggctggc
     1981 taacaaattt accaccattg aagataaata gcgattatag tgtgttcaaa gattatctgc
     2041 cggaaaatgt ggaaaaaggg aaagattatc ttctatatcg ggctgaccaa aacacccctc
     2101 gtacaccgcc aatgcgttta ggttttcgct ttaatgcgga atttacccca aattggtcgg
     2161 gggatttaga gctgatccgc acctttaccc aacgccgtac gagccagttg gaatatatta
     2221 ccgaaggtaa tacgatgttg aatgtcgggt tgtcgtatag caataagtgg aaagagctgg
     2281 attacaaaat cagtctaaac ggcacaaact tactgaatca gccggtgtat atccacactt
     2341 cgtatcacca atttgtacca caaatgggac gaaattttat gttgggaatg gagatgaaat
     2401 tttaagttct cgacataaca agacaagcgg ttattttttc ttcaaatttt gcaaaaaaac
     2461 ggctaaaaat aaccgcttgt ttaaaactca ggtattggta atttggataa aatagtgctg
     2521 tttttccttt cttgttattt tcggagctta ccaaaatttc ctgtttgttg ttaaataaga
     2581 caattaactt tatcccttta ttaaaa
//
LOCUS       DANLXS010000250         2571 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.256, whole genome shotgun sequence.
ACCESSION   DANLXS010000250 DANLXS010000000
VERSION     DANLXS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.256"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..395)
                     /locus_tag="PXD71_002532"
     CDS             complement(<1..395)
                     /locus_tag="PXD71_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL6292591.1"
                     /translation="MRSMFKKFFGKNKSQSAVKSDTIFSIEQDNEGWLYPEKASQLLN
                     TELRQKYLGLLWQQVSMNRQMFEVLYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKVRQSYILPPNVAPEEQSKQR"
     gene            complement(499..963)
                     /locus_tag="PXD71_002533"
     CDS             complement(499..963)
                     /locus_tag="PXD71_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HDL6292592.1"
                     /translation="MMNTQQPKFDLYQHITDRIIAALEQGTAPWLKPWSHPECNMALP
                     RNAVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRP
                     NEREKLDEEGNIVYDEEGNPEMEQHFFIAKHNLFNLDQCENLPAELYDPVIP"
     gene            1172..1435
                     /locus_tag="PXD71_002534"
     CDS             1172..1435
                     /locus_tag="PXD71_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme-like superfamily protein"
                     /protein_id="HDL6292593.1"
                     /translation="MRPVTRNNRGTSFDPRHNKYINTLATALLARWHIEQFSERNIPV
                     NDENLYMAHNIGLDGFHRALLGQSTRKDIENMRKNGMKPWMSI"
     gene            complement(1519..2544)
                     /locus_tag="PXD71_002535"
     CDS             complement(1519..2544)
                     /locus_tag="PXD71_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL6292594.1"
                     /translation="MTAGDTPHDTLKTLVGALKDTREEARKANERGDRFEQEVLRLQA
                     REAEVDSRIAGEVESRVESRLAAAWDGFNRQIEELKGRVQSGNQQQKEAEAIPVGGAS
                     SGFGTESAGQTGNVDNNGIRWVDPDDMVVTDEQGKPVTGNYSGKTKTGFPSPFKAEGE
                     SSNTGTALNNNAGTENMTGNTQIQQIKRTPYYTIPENSTLMGSTAMTALLGRVPIQGA
                     VTDPFPFKVLIGRENLIANGIDLPDVEGAIVSGTSTGDWTLSCVRSEVTSITFVFADG
                     RISNGRASGESRAVKRNQASNLTPFCLTVSLSGFNDIPHSDGNPGFLTMSAVDYVDVY
                     LMSHLAR"
BASE COUNT          706 a          580 c          494 g          791 t
ORIGIN      
        1 tctcgttgct ttgattgttc ttcgggagct acattcgggg gcaaaatata gctttgacga
       61 actttagctg aaaaagcaat aacttctaaa ccgtgatcaa gcattccacc gatatgtgaa
      121 tgatggtgtg attcagaagc tggtaatagt tgaaccatct cagcgtaacg ttctattggc
      181 tgctgataaa gtacctcaaa catttgacgg ttcattgata cctgttgcca aagtagccca
      241 agatattttt gtcgtaattc tgtattaagt aattgagagg ctttttccgg gtataaccac
      301 ccttcattat cttgctcaat agaaaaaata gtatcggatt tgaccgcact ttgagattta
      361 tttttaccaa aaaatttttt gaacattgaa cgcattggaa gagaccgaaa atatgaatgc
      421 tgtattgaat gaaagccgtt tagccctttg cccttttggg taagaccttt ccccttggaa
      481 aggttactct tatccccctt aggggattac aggatcgtac agttccgccg gtaaattttc
      541 acactggtct aaattgaaca gattgtgctt agcaatgaaa aagtgttgtt ccatttccgg
      601 atttccttcc tcgtcataca caatatttcc ctcctcatcg agtttttctc tctcattagg
      661 acggtagttc atcacaacag tagctttttc ccctttacgg atatatccgc cgagttcatt
      721 ggcttttttc gccgtaatcc acttacactg tttgtagccg tgttcagcgg tagcaatcca
      781 aagcaataac gtattgatac cggaatacag acggttagaa accgcattgc gaggtaatgc
      841 catattgcat tccggatggc tccacggttt taaccacgga gctgtacctt gttccaatgc
      901 agcaatgatt ctatcagtaa tatgttggta taaatcaaat ttaggttgtt gagtgttcat
      961 catgtgtttc tccaaagttt gggtaaaaaa atggggaaac acggttaccc tatggggaac
     1021 ttgtttcccc gatgggtagt ttataaaatt tcaatggcca caatttttct agaatcatca
     1081 aagccaacag aactgcctat ggcgtagtaa ttacgaatag tctgttcatc ggcattgatt
     1141 agcagatacc tatcgaggaa gagagattgg gatgagaccg gtaacgagaa ataatcgtgg
     1201 aaccagtttt gaccctcgtc acaataaata catcaataca ttggcaaccg cattattggc
     1261 acgttggcat attgagcaat tctctgagcg taatatccca gtcaatgatg aaaatctcta
     1321 tatggctcac aatattggct tagatggttt ccatcgagcg ttattagggc aatctactcg
     1381 taaagacatt gagaatatgc gtaagaatgg gatgaaacct tggatgtcca tttagctctg
     1441 ttttcaaatg acctaatcca gtttgttccg gagagagtac cgaaattttg cactctttca
     1501 tccgtggtgt atcaaacttc aacgtgctaa atgagacatt aaatatacat caacataatc
     1561 taccgcactc atcgttaaga aaccgggatt cccatcagaa tgaggaatat cgttaaagcc
     1621 cgataaggag acggttaaac aaaatggtgt taaattactt gcttgatttc gtttcacagc
     1681 tctgctttct ccactcgctc gcccattcga aatacgaccg tctgcaaaca caaaggtaat
     1741 gctcgttacc tcagaacgaa cgcaagatag cgtccaatct ccggttgatg taccggatac
     1801 aatcgccccc tcaacatcag gtaaatcaat gccattggca attaaatttt ctcgcccgat
     1861 taatacttta aacggaaacg gatctgtcac agcaccttga ataggtacac ggcctaacaa
     1921 tgccgtcatc gcagtagaac ccattaaagt cgaattttcg ggaatagtgt aataaggtgt
     1981 acgctttatt tgctgtatct gagtattacc agtcatattt tctgtcccag cattattatt
     2041 taatgctgtc ccagtatttg aactttcccc ctctgcttta aaagggcttg gaaagccggt
     2101 ttttgttttc ccggaataat tgcctgtaac cggtttacct tgttcatcgg ttaccaccat
     2161 atcatcagga tcgacccagc gaatgccatt gttatctaca ttccctgttt gtcctgctga
     2221 ttcagtccca aagccagaag aagcccctcc tacagggatt gcctctgctt ctttctgctg
     2281 ctgattacca ctttgaaccc gacctttcag ttcttcgatt tggcgattaa atccatccca
     2341 agcagcagcc aaacggcttt ctacacgact ttctacttca cccgcaatac gagaatccac
     2401 ttcggcttct cttgcttgta agcgtaatac ctcttgctca aagcgatcac cacgctcatt
     2461 tgctttacga gcttcttcac gagtatcttt taatgctcct accaacgttt ttaatgtatc
     2521 gtgaggcgta tctcctgcag taatacctaa tgtattcgcc tcctcatcac t
//
LOCUS       DANLXS010000251         2562 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.257, whole genome shotgun sequence.
ACCESSION   DANLXS010000251 DANLXS010000000
VERSION     DANLXS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.257"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            101..1393
                     /locus_tag="PXD71_002536"
     CDS             101..1393
                     /locus_tag="PXD71_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6292595.1"
                     /translation="MEKSVPIFNETELRANQLLTEKLLIQAIYSRRLANMEKLTAIYR
                     DFTHIDPVLLRYAQGKIALLKGDYAQAIQHFQTILAKQPKLNTVRIELAIALLYDQQL
                     NQAQAQFEKAKSAEHLPPKVHALIEQYQQHLAKKQEWKFSFSANYVRDGNINRTSKQP
                     NIEQTGYVKQPNLLPQTAHGVHYAFGLQRDFNLFDSHYVSFDNQTQGELYWDNHDYDE
                     ITNRTLFGYAYKKSDQTTRLLPFYEKRWKHHRSERWTNGIRLEHAQWFSPHWQLATAL
                     EYGKKRYFDLPIYNGSYKLASATLLWLRNPQQFFFVGTDFNQDKTQLKQYSSDTKGIR
                     FGWQREWQALELSSRLTLGYTDRRYKDTAILGGKLNLGRIRHDRVQSANLTLWKRDWH
                     WLGLMPKLQFSYAKHRSNLPSLYAYTDKNINLIIESRF"
     gene            complement(1643..1966)
                     /locus_tag="PXD71_002537"
     CDS             complement(1643..1966)
                     /locus_tag="PXD71_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HDL6292596.1"
                     /translation="MQKAYQIYTHSEREKVTEAKLELGKVETRLRNEGKTKEEIAQHP
                     EYLQLKRDLNDKQQTFDNAYGTGSKTKRAVDAVTAALQGLAAKDSGQAAVGLASPYLN
                     EQIKK"
     gene            complement(1935..2462)
                     /locus_tag="PXD71_002538"
     CDS             complement(1935..2462)
                     /locus_tag="PXD71_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL6292597.1"
                     /translation="MRDKNKERQQTLERYQELYDQDPELRVIAANSEGDLDLLQETFF
                     VKGELTITDSTPKEKWNWLHMCNLNSYSPSPLSVVEFYIKHGVDVNAQDCYGMTPLHY
                     AMRAKNVDAAIALLNAGADPNIENKDQVIPLSMIGAVPKEIELIEHDISEVKETQELV
                     KGIGEIAEGLSDLHA"
BASE COUNT          735 a          608 c          450 g          769 t
ORIGIN      
        1 ggcacttttc ccaatgatgt tatttgcccg agatcctaat actacacagg cttatattga
       61 gcatactgca cagccaacta caacccccca aagggtcaag atcgaaaaat cagtgcctat
      121 ttttaacgaa actgaattac gtgctaatca attattgacc gaaaagctac ttatccaagc
      181 tatttatagt cgtaggcttg ctaacatgga aaaattaact gcgatttatc gggattttac
      241 ccacattgac ccggttttac tgcgttatgc acaaggtaaa attgccctac tgaaaggcga
      301 ttacgcacag gcaattcagc acttccaaac cattcttgcc aaacaaccca aactaaacac
      361 tgtaagaatt gagctggcga ttgctctatt gtacgatcag caacttaatc aagcccaagc
      421 acaatttgaa aaagccaaaa gtgccgaaca tctaccgcct aaagttcacg cattaattga
      481 gcaatatcag caacatctgg caaaaaaaca ggaatggaaa tttagttttt cagccaatta
      541 tgtgcgagac ggcaacatta accgaacctc gaaacagccg aacattgagc aaaccggcta
      601 tgtaaaacaa cctaatttgt tgccccaaac cgcacatggt gttcactatg cgtttggatt
      661 acagagagat ttcaacctgt tcgactcaca ctatgtgagt tttgacaacc aaacccaagg
      721 tgaactttat tgggataacc acgattatga tgaaattacc aatcgcacct tgtttggata
      781 cgcttacaaa aaatccgacc aaaccacccg cttgttaccg ttttatgaaa aacgctggaa
      841 acatcaccgt tcggaacgct ggacaaacgg aattcgactt gaacacgcac aatggttttc
      901 accgcattgg caactcgcca ccgcattgga atacggcaaa aaacgttatt ttgatttgcc
      961 gatttataac ggcagctaca aacttgcctc tgccacattg ctttggttac gcaatccaca
     1021 acaatttttc tttgtcggca ctgatttcaa tcaagacaaa actcagttaa aacaatatag
     1081 ctctgatact aaaggcatac gctttggctg gcaacgagaa tggcaagccc tcgagctttc
     1141 cagtcgcctc acgcttggct atactgacag acgttacaaa gacaccgcta tattgggagg
     1201 aaaacttaat cttggcagaa tccgccatga ccgtgtgcaa agtgctaacc tcacgctttg
     1261 gaaacgagat tggcactggc tcggcctgat gccgaagctg caatttagtt acgccaaaca
     1321 ccgtagcaac ctgccgagcc tctatgccta caccgataaa aacatcaatt taattattga
     1381 aagtcggttc tagctccata aaaacaacaa gcggttaaaa ttcctagaaa ttttgcaaat
     1441 tgcaaaaaat gtagaaaatt taaccgcttg tatctatact gcagcttctt ttccttcagt
     1501 ataaattaat atacaccgtt gcaacaataa tggctgctcc ctgtcatcac cttgaactga
     1561 aaattgctca aggtggatat gactatcgca gccgcttcca ccgcacccag taatgtatgg
     1621 gcaagcaggt tggcttgggt atctactttt taatttgttc gttgagatat ggcgaagcta
     1681 agcctactgc tgcttgtcct gaatctttcg ccgctaaacc ttgtaaggct gccgtcaccg
     1741 catccaccgc acgcttggtt ttgctgcccg tgccgtaggc gttatcaaaa gtctgctgtt
     1801 tatcgtttaa atcccgtttt aattggagat attcaggatg ttgagcgatt tcttcttttg
     1861 tttttccctc atttctcaaa cgggtttcca ctttgcccaa ttccagcttc gcttccgtca
     1921 ctttttcacg ctcactatgc gtgtaaatct gataagcctt ctgcaatctc gcctatgccc
     1981 ttcaccagct cctgtgtttc tttcacctca ctaatatcgt gttctattaa ctctatctct
     2041 ttaggaactg ctccaatcat agacaatgga ataacttgat ctttattttc aatattcgga
     2101 tctgcccccg catttaataa tgctatcgct gcatcaacat tctttgccct cattgcataa
     2161 tgtaaagggg tcattccgta gcaatcttgg gcattaacat ctaccccatg tttaatgtaa
     2221 aattcgacta cagaaagagg agatggtgaa taactattta agttacacat atgtagccag
     2281 ttccattttt cttttggtgt gctatccgta atagtcagtt caccttttac gaaaaatgtt
     2341 tcttgtaaaa gatcgagatc gccttctgag tttgctgcaa taactcttaa ttcaggatct
     2401 tgatcatata attcttgata acgttctaat gtctgctgac gttctttatt cttatctctc
     2461 attttacttt cctatttgtt ggaatttttt catgtcatcg attattgatt taatattatc
     2521 tttccccggc atctcattct tatgactccg attttcagat at
//
LOCUS       DANLXS010000252         2533 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.259, whole genome shotgun sequence.
ACCESSION   DANLXS010000252 DANLXS010000000
VERSION     DANLXS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.259"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            79..1194
                     /gene="wecB"
                     /locus_tag="PXD71_002539"
     CDS             79..1194
                     /gene="wecB"
                     /locus_tag="PXD71_002539"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDL6292598.1"
                     /translation="MRYLIVFGTRPEAIKMAPLVEGFKKSGLNFKVCVTAQHRQMLDQ
                     VLELFEITPDYDLDIMSNKQTLSTVTSAILEKIQPVIAEYKPTTIFVHGDTATTLASS
                     LAAYYNQIDIAHIEAGLRTHNIYSPWPEEGNRKLTAALAKYHFAPTQSTKKNLLNENI
                     AEGTIYVTGNTVIDALFLACKKLDENIDLLSSLQEKFSYIKSKRTILITGHRRENFGN
                     GFENICKAVSTLASDFPDVQFIYPVHLNPNVREPVNRLLADKSNVHLIEPCDYLSFVY
                     LMRESYLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVEAGTVKLVGTAQSEIIKQV
                     TELLNDQAAYTKMSEAHNPYGDGTAVKQILSVFQSKQ"
     gene            1209..2480
                     /gene="wecC"
                     /locus_tag="PXD71_002540"
     CDS             1209..2480
                     /gene="wecC"
                     /locus_tag="PXD71_002540"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL6292599.1"
                     /translation="MSNFNTISVVGLGYIGLPTATVFAQHGLNVIGVDVNQHAVDHIN
                     QGKIHIVEPDLDVAVHQCVSSGKLKATLTPEPAEAFLIAVPTPFKGEDYEPDLSYIQA
                     ACKAIAPVLEKGNLVILESTSPVGATEQMAEWLAEYRPELSFPQQAGEDSDIRIAHCP
                     ERVLPGQVMHELIENDRIVGGMTNKCSVQAVNLYKTFVKGDCIVTNARTAEMCKLTEN
                     SFRDVNIAFANELSIICDRLDINVWELIALANRHPRVNILQPGCGVGGHCIAVDPWFI
                     VNKTPDLAKIIRTAREVNDHKPEWVISKMNEAVIYALQKTGKSIDQIKIACLGLAFKP
                     DIDDLRESPALKITEKLAEKYPNQVFAVEPNVEELPKKLANKNISLISVDEALEVADV
                     VVVLVDHSEFKLVKPVFLNDICVVDTKGIWR"
BASE COUNT          814 a          485 c          492 g          742 t
ORIGIN      
        1 gatctttata actaatttaa caaaattact tacatctagt gtattttagt tttatctata
       61 tttttaaagg agtaataaat gcgctatctc atcgtcttcg gtactcgtcc tgaggcaatc
      121 aaaatggctc ccttagttga gggctttaag aaaagcggtc taaattttaa agtctgtgtt
      181 actgctcagc accgccaaat gctggaccaa gttttagaat tattcgaaat tactccagat
      241 tatgacctgg atattatgag caataagcaa acactttcta ctgtaacttc tgctatttta
      301 gaaaaaattc aacctgttat tgccgaatac aaaccaacta ctatttttgt tcatggcgat
      361 acagcaacca ctcttgcctc ttcattagca gcctattaca accaaattga tatcgctcat
      421 attgaggcag gtctaagaac gcataatatc tactctcctt ggccggaaga aggcaatcgc
      481 aaattaacag ctgctttggc aaaataccat ttcgcaccaa cacaaagcac caagaaaaat
      541 ctactcaacg aaaatattgc tgagggcacc atttatgtaa cgggtaatac ggtaattgat
      601 gccttattcc tcgcttgcaa aaaattagat gaaaatattg atttattaag ctcgctacaa
      661 gagaaatttt cttatataaa aagtaagcgt actattttga tcaccggaca tcgccgcgaa
      721 aatttcggta atggttttga gaacatctgt aaagccgttt caacattagc gtctgatttc
      781 cctgacgttc aatttatcta ccccgtacac ctcaacccaa atgtaagaga accggtcaat
      841 cgcctattag cggataaatc caatgtgcat ctcattgagc cttgcgacta tctctccttt
      901 gtttatttaa tgagagagtc ttaccttatc ttaactgact ccggaggtat tcaagaagaa
      961 gccccatcat taggaaaacc ggtattagta atgagagaca caacagaaag accggaagct
     1021 gtagaagccg gaacagtaaa acttgttgga actgcacaat ctgaaattat taaacaagtt
     1081 actgagttac tcaatgatca agctgcttat acaaaaatgt ctgaggctca caacccttat
     1141 ggcgatggca cagctgtgaa acaaattctc tcagtatttc aatcaaaaca ataataaggg
     1201 ataaatgtat gtcgaacttt aataccattt cagtagtagg cttaggctat attggcttac
     1261 caaccgccac tgtatttgcc caacacggtc taaatgtaat tggcgtagat gttaatcagc
     1321 acgccgtaga ccatatcaat caagggaaaa ttcacattgt cgagcctgat ttagatgttg
     1381 ctgttcacca atgtgtatct agcggaaaat taaaagccac actgacacct gaaccagcag
     1441 aagcattttt aattgctgta ccaacgcctt ttaaaggcga ggattatgag cctgatttaa
     1501 gttatattca agctgcttgc aaagcaattg cacctgtgct ggaaaaaggt aatttagtca
     1561 ttttagaatc aacctctccg gttggtgcaa cagaacagat ggctgagtgg cttgccgagt
     1621 accgtccaga gctaagcttc cctcagcaag caggtgaaga ttctgatatt cgtatcgccc
     1681 attgcccaga acgagttcta ccaggacaag tgatgcatga attgattgaa aacgaccgta
     1741 ttgtgggtgg tatgacaaat aaatgctctg tgcaagcggt caatttatac aaaacttttg
     1801 taaaaggcga ctgtatagtt accaatgccc gtaccgcaga aatgtgtaaa ttaaccgaaa
     1861 atagtttccg tgatgtcaat atcgcttttg caaatgagct ttctattatt tgtgatcgcc
     1921 tagatattaa tgtatgggaa ctaattgctt tggctaacag acaccctcga gtcaatatct
     1981 tacaaccggg ttgtggtgta ggtggacact gcattgctgt agatccttgg tttattgtga
     2041 ataaaactcc tgacttggca aaaattatcc gcactgctcg tgaggtaaac gatcataaac
     2101 ctgaatgggt tatctcaaag atgaatgaag cggtaattta tgccttacaa aaaacaggta
     2161 aatctatcga tcaaatcaaa attgcttgtt tagggttggc attcaagcct gatattgacg
     2221 atttgcgtga aagtccggca ttaaaaatta ctgaaaaatt ggcggaaaag tatccaaatc
     2281 aagtatttgc agttgagccg aatgttgaag agctaccgaa aaaattggct aacaagaaca
     2341 tttctttgat ttctgttgat gaagctctgg aagtagctga tgttgtagtt gttttggtgg
     2401 atcatagcga atttaaacta gtcaaacctg tgtttttgaa tgatatttgt gtggtggata
     2461 ctaaaggaat ttggagataa gtgatgaaaa agaaacagca atatcgtaaa tccactatta
     2521 acaatactaa tgc
//
LOCUS       DANLXS010000253         2520 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.260, whole genome shotgun sequence.
ACCESSION   DANLXS010000253 DANLXS010000000
VERSION     DANLXS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.260"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            126..2402
                     /gene="gshAB"
                     /locus_tag="PXD71_002541"
     CDS             126..2402
                     /gene="gshAB"
                     /locus_tag="PXD71_002541"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL6292600.1"
                     /translation="MKLQKLIRENHLALLFQQGNFGLEKESQRVDRNGNIVTTPHPAV
                     FGNRSYHPYIQTDFAESQLELITPPNAKLEDSFRWLSAIHEVVLRSLPEEEYIFPLSM
                     PAGLPPEEQIQEAQFDNPEDVQYRQHLSKNYGKYKQMVSGVHYNFQLSTEFVEKAFAL
                     QTEYSELKAFQNALYMKLANNFLRYQWILLYLLAATPTVESQYFYEKSPLARGQLVRS
                     LRSSPYGYVNSEKVVVNHDSLESYVESLEANVANGLLIAEKEFYSNVRLRGGKKAREL
                     LENGVKYAEFRLFDLNPFAPYGIELNDAKFIHYFLLGMLWLEETSGQKEVEIGKRKLY
                     QVALEDPRTETAFRAEGEAILNQLLAMLEELSAGQESLDLVREKLAQFADPSKTLNGR
                     LIAAIEEVGSYKALGAKLAQTYKAQAFERFYALSAFDNMELSTQALFFDLIQQGIEVE
                     VLDENDQFLALKFGDHLEYVKNGNMTSRDQYISPLIMENKVVTKKVLAKAGFNVPKSV
                     EFTNLEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQAVKNREDFAKAVEIAFREDK
                     EVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVVGDGIHSVKELVERKNDDPLRGDGS
                     RSPLKKIALGEIEQLQLKEQGLTVDSVPAKGQTVQLRANSNISTGGDSIDMTDQMHES
                     YRQIAVGIAQAMGAKVCGVDLIIPDLTKPAEPNLSSWGVIEANFNPMMMMHIFPYQGK
                     SRRLTKAVIKMLFPEIGN"
BASE COUNT          767 a          516 c          550 g          687 t
ORIGIN      
        1 gaagcagtat ttctgttata accaaatcat ctacttcatt ccaataaact aaaacccact
       61 atttcactaa gtctttcatt tgtgttattg tgccgataca tttttttgac tacaaaggat
      121 agatgatgaa actacaaaaa cttatccgag aaaatcattt agcattgctg tttcaacaag
      181 ggaattttgg gcttgagaaa gagagccaac gggttgatcg caacggcaat atcgttacta
      241 ctccccaccc tgccgtattc ggtaatcgct cttaccaccc ttatattcaa accgattttg
      301 ccgaaagtca gctagagtta atcactcccc cgaatgctaa attagaagac agttttcgtt
      361 ggctctctgc tattcacgaa gtggtattgc gttcgttgcc agaggaggaa tatatcttcc
      421 ctctcagtat gccggcaggc ttgccgccgg aagagcaaat tcaagaagca caatttgaca
      481 accctgaaga tgtgcaatat cgccaacacc tttccaaaaa ctatgggaaa tataagcaaa
      541 tggtaagcgg cgtgcattat aattttcaac tttcaacgga atttgtcgaa aaagcctttg
      601 ctttacaaac ggaatatagc gagctgaaag ccttccaaaa cgcactttat atgaagttag
      661 ctaataactt tttgcgttat caatggattt tgctctattt attggctgcc acgcctacgg
      721 tggaatcgca atatttttat gaaaaatcac cgcttgcgag agggcaactt gtccgcagct
      781 tgcgttcaag cccttatggt tatgtgaatt cggaaaaagt ggtggttaat cacgacagct
      841 tagaaagcta tgttgaaagc cttgaagcca atgttgccaa cggattatta attgccgaaa
      901 aagaattcta ttccaatgtg cgtttacgag ggggcaaaaa agcccgtgag ctattggaaa
      961 acggtgtgaa atacgccgaa ttccgtttat ttgatttaaa ccctttcgcc ccttacggca
     1021 ttgagctgaa tgatgccaaa tttatccatt atttcttact cggtatgctt tggctggagg
     1081 aaacaagcgg tcaaaaagag gtcgaaatcg gcaaacggaa actttaccaa gtcgcacttg
     1141 aagatccaag aacagaaacc gctttccgtg ccgaaggtga agccatttta aatcaacttt
     1201 tagcgatgct tgaagaatta tctgccggtc aggaaagcct agatttggtg cgggaaaaat
     1261 tagctcaatt tgctgatcca agcaaaaccc tgaacggacg tttaattgcc gcaatagagg
     1321 aagtcggcag ttataaagca ttgggggcta aattggctca aacctataag gcacaagcct
     1381 ttgagcgttt ttatgcgtta tcggcatttg ataatatgga actttccact caagccctgt
     1441 tttttgattt gatccaacaa gggattgagg tggaagtact ggacgaaaac gatcaattcc
     1501 tcgccctaaa atttggcgat catttggaat atgtgaaaaa cggcaatatg accagccgag
     1561 atcaatacat ttcaccgctg attatggaaa acaaagtggt gacgaaaaaa gtattggcaa
     1621 aagccggctt taacgtgccg aaaagcgttg aatttaccaa ccttgaacag gctgtggcac
     1681 attatccgct atttgaaggc aaagcggtgg tgattaagcc gaaatcgact aactatggtt
     1741 tagggattac catcttccaa caagcggtca aaaatcgaga agattttgca aaagcggttg
     1801 aaattgcgtt ccgtgaagac aaagaggtga tggtggaaga ctatttagtc ggtactgaat
     1861 accgcttctt tgtactgggc gatgaaaccc ttgcggtgtt actgcgtgta ccggcgaatg
     1921 tagtgggaga tggcattcac agtgtaaaag agctggtgga gcgtaaaaat gacgatccgc
     1981 tacgtggcga tggctctcgt agcccgttga aaaaaattgc cttaggtgaa attgagcagt
     2041 tacaacttaa agagcaaggc ttaaccgtag attccgtacc ggcgaaaggt caaacagttc
     2101 aactgcgtgc caattccaac atcagcaccg gcggcgattc gattgatatg accgaccaaa
     2161 tgcacgaaag ctatagacaa attgcagtgg gaattgctca ggcaatgggg gctaaagtgt
     2221 gtggggtgga tttgatcatt cctgatctca ccaaacctgc cgagccaaat ttaagctctt
     2281 ggggcgtcat tgaggcaaac ttcaacccaa tgatgatgat gcacattttc ccttaccaag
     2341 gcaaatcccg ccgtttaacc aaagcggtaa tcaaaatgct gtttcctgag ataggcaatt
     2401 aaattttctc tctatacctc ttttaaggag aatttactca agaattctcc ttaatttatt
     2461 tctaaagttt tactccaatt aaatttcact ttgtgcaaaa cactccaaaa gaagaatttt
//
LOCUS       DANLXS010000254         2480 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.261, whole genome shotgun sequence.
ACCESSION   DANLXS010000254 DANLXS010000000
VERSION     DANLXS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.261"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            26..1093
                     /gene="hisC"
                     /locus_tag="PXD71_002542"
     CDS             26..1093
                     /gene="hisC"
                     /locus_tag="PXD71_002542"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL6292601.1"
                     /translation="MTISQLSRKNIQALTPYQSARRLGGSGDVWLNANEYALSPNFDL
                     TDRTFNRYPEPQPQAVIEGYARYAGVAPENVLVSRGGDESIELIIRAFCEANDSILYC
                     PPTYGMYAVSADTCGIATKTVPLTADFQLDLAQIKANLEGVKVVFVCSPNNPTGNLIK
                     RSDLFELLQITAGKAIVVVDEAYIEFCPEARLVSELANFPHLAIIRTLSKAFALAGLR
                     CGFTLANAELIGVLQKVIAPYPLPVPVSDIAAQALSPQGIEQMKTRVAEVITLRAELQ
                     KNLENLPLVEQVFDSEANYLLFKCQDGQKVFKALWEQGIILRDQHKALGLQNCIRITV
                     GAREENLRVVEAITALSQFQR"
     gene            1278..2366
                     /gene="hisB"
                     /locus_tag="PXD71_002543"
     CDS             1278..2366
                     /gene="hisB"
                     /locus_tag="PXD71_002543"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDL6292602.1"
                     /translation="MQPTLFIDRDGTLIDEPKTDFQIDSLEKLKFEKNVIPALLKLKG
                     KYRFVMVTNQDGLGTSSFPQADFDKPHNAMMALFNSQGIEFDDVLICPHKPEDGCDCR
                     KPHTKLLQKYIDRQLFDPANSFVIGDRATDVQLAENLGIRALQYHPEKLNWDLIAEKL
                     LGEAVTNIGERPKRFAEVIRKTKETDIKVQVWLDETGNNEISTGVGFFDHMLDQIATH
                     GGFRMNITTKGDLWIDEHHTVEDTALALGTALKQAIGDKRGIARFGFVLPMDESRAEC
                     AMDLSGRPFIKFKADFKRDKVGDFSTELTEHFFQSIAFTMLATLHIKAKGDNDHHKIE
                     SLFKVFGRTLRQCIRIEGNELPSSKGVL"
BASE COUNT          697 a          617 c          602 g          564 t
ORIGIN      
        1 tgtataaatt atacaggaac acaatatgac aatttcacaa ctttcccgta aaaacattca
       61 agctctcacc ccttaccaat cggcacggcg tttggggggt agtggcgatg tgtggctgaa
      121 tgccaatgaa tatgcccttt cgccaaactt tgatttaacc gaccgcactt ttaaccgcta
      181 ccctgaacct caaccgcagg cggtgattga aggttacgcc cgatatgcag gcgttgcacc
      241 tgaaaacgtg ctggtttccc gtgggggcga tgagagcatt gagctgatta tccgagcctt
      301 ttgcgaggca aacgacagca ttctctactg cccaccaacc tacggaatgt acgccgtgag
      361 tgccgacacc tgtggcattg cgaccaaaac tgtgccttta accgccgatt tccagttgga
      421 tttagcccaa atcaaagcta acctcgaggg cgtgaaagtg gtgtttgtgt gcagcccgaa
      481 caacccgaca ggcaacttaa tcaagcggtc ggatttgttc gaacttttgc aaatcaccgc
      541 aggcaaagcg attgtggtgg tggacgaagc ctacatcgaa ttttgccctg aagctcgctt
      601 ggtaagcgag ctggcgaatt tcccacattt ggcgattatc cgcaccctat ccaaagcctt
      661 tgccttggcg ggtttgcgtt gtggctttac cttggcaaac gctgagctga tcggcgtgtt
      721 acaaaaagtg attgccccgt atccgctgcc tgtgcctgtt tccgacatcg ccgcccaagc
      781 cctctcccca caaggcattg agcagatgaa aacccgtgtg gcggaagtca tcaccctgcg
      841 tgccgaattg caaaaaaatc tggaaaactt accgcttgta gaacaagttt ttgacagcga
      901 agccaactac ctcctattca aatgccaaga cggacaaaaa gtcttcaaag cactttggga
      961 gcaaggcatt attttgcgtg accaacacaa agcgttagga ttgcaaaatt gcattcggat
     1021 tacggtgggg gcgagagagg agaatttgag ggtggttgag gcgattacag cactgagcca
     1081 atttcagaga tagaatttaa cgagcagtat tttaagagac atttatagcc tagcacggca
     1141 tagccgtact aggttacgca taagtcagtc cctttgggac tgaatacaga gaggcaaagt
     1201 ctctcactaa tgcttaaccc catatgcctt ggcgtgtggg gagagtaatg aataagatac
     1261 agatacagga gaaaccaatg caacccaccc tttttatcga ccgtgacggc acgttaattg
     1321 atgagccaaa aaccgatttt caaatcgaca gcttggaaaa gctgaaattc gagaaaaacg
     1381 tgattcctgc cctgctcaaa ctcaagggca aataccgctt tgtgatggtt acgaaccaag
     1441 acggcttagg cacgagttcg ttcccacagg cagatttcga caaaccgcac aatgcaatga
     1501 tggcgttgtt caactcgcaa gggattgaat ttgatgatgt gctgatttgt ccacacaagc
     1561 ccgaagacgg ttgcgattgc cgcaaaccgc acaccaaact gctacaaaaa tatatcgacc
     1621 gccaactgtt cgacccagcc aacagctttg tgattggcga ccgtgctacc gatgtgcaac
     1681 tggcggaaaa ccttggcatt cgggcgttgc aatatcaccc tgaaaaactg aactgggatt
     1741 tgattgctga aaaactactg ggcgaagccg tcaccaacat tggcgagcga ccaaagcgct
     1801 ttgccgaagt tatccgtaaa accaaagaga ccgacatcaa agtgcaagtg tggctggacg
     1861 aaacaggcaa caacgaaatc agcaccggcg tgggcttttt cgaccatatg cttgaccaaa
     1921 ttgccaccca cggcggtttt agaatgaaca tcaccaccaa aggcgacttg tggattgacg
     1981 aacatcacac ggtggaagac accgccctcg ccctcggcac cgcattaaaa caagcgattg
     2041 gcgacaaacg cggcatcgcc cgtttcggct tcgtgttgcc gatggacgaa agccgtgcgg
     2101 aatgtgcgat ggacttatca ggcagacctt tcatcaagtt caaagcggat ttcaaacgcg
     2161 acaaagtggg cgatttcagc accgagctca ccgaacactt cttccaatcc atcgccttca
     2221 cgatgctcgc caccctgcac atcaaggcaa aaggcgacaa cgaccaccac aaaatcgaaa
     2281 gcctgttcaa agtcttcggc agaacactca gacagtgcat tcgtattgaa gggaatgaac
     2341 taccaagcag taaaggtgta ttataatcac cgtaggggcg ggttttaacc cgcccgaaag
     2401 tgcggttgaa atttgcaaag tttttgggga ttttgaccgc ttgttataaa ggatatattg
     2461 atgattagag aagttattga
//
LOCUS       DANLXS010000255         2472 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.262, whole genome shotgun sequence.
ACCESSION   DANLXS010000255 DANLXS010000000
VERSION     DANLXS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.262"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            38..1513
                     /gene="msr(E)"
                     /locus_tag="PXD71_002544"
     CDS             38..1513
                     /gene="msr(E)"
                     /locus_tag="PXD71_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(E)"
                     /protein_id="HDL6292603.1"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
     gene            1569..2453
                     /locus_tag="PXD71_002545"
     CDS             1569..2453
                     /locus_tag="PXD71_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="HDL6292604.1"
                     /translation="MTIQDIQSLAEAHGLLITDKMNFNEMGIDFKVVFALDTKGQQWL
                     LRIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAE
                     TYEIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLV
                     KSEIGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAH
                     IDDPAIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFAL
                     ETQNESLIVGAKAQLGVI"
BASE COUNT          847 a          406 c          532 g          687 t
ORIGIN      
        1 attaaaaatt tcaagagcta aacaggagta aataaaaatg agtttaatta ttaaagcgag
       61 aaacatacgc ttggattatg ctgggcgtga tgttttggat attgatgaat tggaaattca
      121 ctcttatgac cgtattggtc ttgtgggtga taacggagca ggaaagagta gtttactcaa
      181 agtacttaat ggcgaaattg ttttagccga agcgacatta cagcgttttg gtgattttgc
      241 acatatcagc caactgggcg gaatcgaaat agaaacggtc gaagaccggg caatgttatc
      301 tcgccttggt gtttccaatg tacaaaacga cacaatgagt ggcggagagg aaactcgtgc
      361 aaaaattgct gccgcatttt cccaacaagt acatggcatt ctagcggatg aaccaaccag
      421 ccaccttgat ctcaatggaa tagatctact tattggtcaa cttaaagcat ttgatggagc
      481 attacttgtt atcagtcatg accgatattt tcttgatatg gttgtagaca agatatggga
      541 gttaaaagac ggtaaaatta cggaatattg gggtggttac tcggattact tgcgtcaaaa
      601 agaagaagag cgacaacacc aagccgtaga atatgagctg atgatgaagg aacgggagcg
      661 attagaatct gctgtgcaag aaaaacgcca gcaagctaat cgattagaca ataagaaaaa
      721 aggagaaaaa tccaaaaact ctaccgaaag tgctggacga cttgggcatg caaaaatgac
      781 tggcaccaag caaagaaaac tgtatcaggc agctaagagt atggaaaagc gtttggctgc
      841 attagaagat attcaagcac cagagcattt gcgttctatt cgttttcgtc aaagttcagc
      901 cctagaactg cacaataagt tcccgattac ggcagatggt ctgagcttaa aatttggtag
      961 ccgtactatc tttgatgacg ctaactttat aataccgctt ggcgctaaag tcgctataac
     1021 tggatcgaat ggaacaggga aaacgtcctt gttaaaaatg atatcagaac gtgctgatgg
     1081 attaaccata tctccaaaag ctgaaattgg ctactttaca caaacaggat ataaatttaa
     1141 cacgcataaa tctgtgctct cctttatgca ggaagagtgc gagtacacag ttgcggaaat
     1201 tcgtgcagta ttggcttcaa tggggatcgg agcgaatgat attcaaaaaa acttatccga
     1261 cttatcggga ggtgaaatca tcaaactgct tttatccaaa atgcttttag gaaaatataa
     1321 tattttgctt atggatgaac caggaaacta tcttgaccta aaaagtattg ccgcattaga
     1381 aacaatgatg aagtcctatg caggaactat tatcttcgta tctcatgaca agcaattggt
     1441 cgataatatt gctgacatta tctacgagat caaagaccac aaaatcatca agacttttga
     1501 gagagattgt taatgatagc caatctaatc cgaacattaa ttattgaact ctttaaagga
     1561 aattaaaaat gacaattcaa gatattcaat cacttgctga agcacacggc ttgttgatta
     1621 cggacaaaat gaatttcaat gaaatgggca ttgattttaa ggtcgttttt gctcttgata
     1681 caaaggggca acaatggttg ctgcgtattc ctcgtcgtga tggcatgagg gaacaaatca
     1741 agaaagaaaa acgcatttta gaattggtaa aaaaacatct ttctgtagag gttcctgatt
     1801 ggagaatttc atctacagaa ttagtggctt atcccatact taaagataat cctgttttaa
     1861 atttggatgc tgaaacctat gaaataattt ggaatatgga caaagatagc ccgaaataca
     1921 taacatcttt ggcaaaaacc ttatttgaaa tccatagtat tcctgaaaaa gaagttcggg
     1981 aaaatgattt gaaaattatg aaaccttcag atttaagacc tgaaatagca aacaatttgc
     2041 agttagtaaa atctgaaatt ggtataagtg agcaattgga aacccgctac agaaaatggt
     2101 tggataatga tgttctatgg gcagatttca cccaatttat acatggcgat ttatatgctg
     2161 ggcatgtact agcttcaaag gatggagctg tttcaggcgt tattgattgg tcaacagccc
     2221 atatagatga cccagcgatt gattttgctg ggcatgtaac tttgtttgga gaagaaagcc
     2281 tcaaaactct aatcatcgag tatgaaaaac tagggggtaa agtttggaat aaactatatg
     2341 aacagacttt agaaagagca gcggcctctc ctttgatgta tggtttattt gccttagaaa
     2401 ctcaaaatga aagccttatc gttggagcaa aagctcagtt gggagttata taatttaaaa
     2461 atatgattgc tg
//
LOCUS       DANLXS010000256         2467 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.263, whole genome shotgun sequence.
ACCESSION   DANLXS010000256 DANLXS010000000
VERSION     DANLXS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.263"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..265
                     /locus_tag="PXD71_002546"
     CDS             <1..265
                     /locus_tag="PXD71_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL6292605.1"
                     /translation="VILKREEVETKSAGGIVLTGSAATKSTRGKVIAVGTGRILDNGS
                     VQPLAVKVGDVVIFNEGYGVKTEKIDGEEVLILAEHDILAIVE"
     gene            334..1974
                     /gene="groL"
                     /locus_tag="PXD71_002547"
     CDS             334..1974
                     /gene="groL"
                     /locus_tag="PXD71_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608625.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL6292606.1"
                     /translation="MAAKDVKFGNDARVKMLKGVNILADAVKVTLGPKGRNVVLDRAY
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNE
                     GLKAVAAGMNPMDLKRGIDKAVAAVVEELKAISKPCETSKEIEQVGTISANSDSTVGQ
                     LIAQAMEKVGKEGVITVEDGTGLEDALDVVEGMQFDRGYLSPYFINKPEAGTVELENP
                     YILLVDKKVSNIRELLPVLEGVAKAGKPLLIIAEDIEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTAGTVISEEIGMELEKATLEELGQAKRVVISKDNTTIID
                     GIGDETQIKGRVAQIRQQIEDSTSDYDKEKLQERVAKLAGGVAVIKVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALVRAANKVADTLKGDNEEQNVGIKLALRAMEA
                     PLRQIVANAGEEASVVARNVKEGSGNYGYNAGTEQYGDMLEMGILDPTKVTRSALQFA
                     ASIAGLMITTECMITDLPKEEKLDPAAMGGMGGMGGMM"
     gene            complement(2099..2425)
                     /locus_tag="PXD71_002548"
     CDS             complement(2099..2425)
                     /locus_tag="PXD71_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDL6292607.1"
                     /translation="MLKRILGITLCTILVSSNAIASVKTSSLTVAKPTLSKNLPTECE
                     KMFNTANKLVSDAERQPGTHTQVLKMKNKLSLTKQQILKMDSELQQKSCEKGLTALNS
                     LKQKHQ"
BASE COUNT          784 a          403 c          600 g          680 t
ORIGIN      
        1 agttatttta aaacgtgaag aagttgaaac aaaatcggca ggcggtattg ttttaaccgg
       61 ttcggcagca acaaaatcaa cccgtggcaa agtaattgca gtaggaacag gtcgcatttt
      121 agacaatggt tctgttcagc ctttagcagt taaagtgggc gatgtagtga tttttaacga
      181 aggctacggc gtaaaaaccg agaaaattga cggcgaagaa gtgctaattt tagcagagca
      241 cgatattttg gcgattgtag aataatttcg ttctgcacaa gcggttaaaa aagcaaaatt
      301 ttttacatat ttcaaataca aaggatttag aaaatggcag caaaagacgt gaaatttggt
      361 aacgatgcac gcgtaaaaat gttaaaaggc gtgaatattt tagccgatgc ggtaaaagta
      421 actttaggtc cgaaaggtcg taatgtggtg ttagatcgtg cttatggtgc accaacgatt
      481 acaaaagacg gtgtgtcggt tgcccgtgaa atcgaattag aagataaatt tgaaaatatg
      541 ggggctcaaa tggtgaaaga ggttgcctct aaagccaatg atgccgccgg tgacggtaca
      601 actaccgcaa ctgtattagc tcaagcgatt gtaaacgagg gcttaaaagc agtggctgcg
      661 ggtatgaacc caatggattt aaaacgtggt atcgataaag cggttgcagc agtggttgaa
      721 gagttaaaag cgatttcaaa accgtgcgaa acctcaaaag aaattgagca agtagggacg
      781 atttctgcaa actcagattc aacagtcggt caattaattg ctcaagcaat ggaaaaagtg
      841 ggcaaagagg gcgtgattac tgttgaagac ggtacaggtt tagaagatgc attagacgtg
      901 gttgagggta tgcaatttga ccgtggttac ttatcgccat actttatcaa taaaccggaa
      961 gccggtacgg ttgagttaga aaacccatat attcttttag tggataaaaa agtgtctaac
     1021 attcgtgaat tattaccggt gttagaaggt gttgcaaaag caggtaaacc gttattaatt
     1081 attgccgaag atattgaagg cgaggcgtta gctaccttag tggtgaacac tatgcgtggt
     1141 atcgtgaaag tggcggcagt gaaagcacct ggctttggtg atcgccgtaa agcaatgtta
     1201 caagatattg cgatcttaac tgcaggtact gtgatttctg aagagatcgg tatggagctt
     1261 gaaaaagcaa cccttgaaga gcttggtcaa gcaaaacgtg tggttatcag caaagataac
     1321 accacaatta ttgacggtat tggtgatgaa actcaaatca aaggtcgtgt agcacaaatc
     1381 cgccaacaaa tcgaagattc aacttcagat tacgacaaag aaaaactaca agagcgtgta
     1441 gcaaaattag ccggtggtgt tgcggtaatt aaagtaggtg cggcaactga agtggctatg
     1501 aaagagaaaa aagaccgtgt tgatgatgct cttcacgcaa cccgtgctgc ggtggaagaa
     1561 ggtattgtac cggggggcgg agttgcttta gtgcgtgcag ccaacaaagt ggctgataca
     1621 ctaaaaggcg ataacgaaga gcaaaatgta ggtatcaaac ttgccttgcg tgcaatggag
     1681 gctccattac gccaaatcgt tgccaatgca ggtgaagaag cctcagttgt agcccgcaac
     1741 gtgaaagaag gttcaggcaa ctacggctat aacgcaggta cagagcagta cggcgatatg
     1801 cttgaaatgg gaattttaga cccaactaaa gtgactcgtt cagcacttca attcgcggct
     1861 tctattgcag gtttaatgat cactacagaa tgtatgatta ccgatctgcc aaaagaagag
     1921 aaattagatc ctgccgcaat gggcggtatg gggggaatgg gcggaatgat gtaattgccc
     1981 attgagtgaa caaaagctac tgaaattaat tcagtagctt ttttgttggt aacaagtggt
     2041 taaatttatg ctattttttg caaataaaaa accaagcaag taagcttggt tttagcgttt
     2101 attgatgttt ttgtttaagg ctgtttagtg ctgttaaacc tttctcacaa cttttttgct
     2161 gtaattcaga atccattttt aggatttgtt gctttgttaa agaaagttta tttttcattt
     2221 ttagcacttg agtatgagta ccaggctgtc tttctgcatc agagaccaat ttatttgccg
     2281 tgttaaacat tttctcacat tcagttggca aatttttact taaagtaggt tttgcaactg
     2341 taagtgatga ggttttaaca cttgctatag cattactgga aaccaatatg gtacaaagtg
     2401 ttattcctaa aattcttttt agcatgcgtt ctccacacat taagtgttta aaagtaaaaa
     2461 tctaata
//
LOCUS       DANLXS010000257         2464 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.264, whole genome shotgun sequence.
ACCESSION   DANLXS010000257 DANLXS010000000
VERSION     DANLXS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.264"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(234..2213)
                     /locus_tag="PXD71_002549"
     CDS             complement(234..2213)
                     /locus_tag="PXD71_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL6292608.1"
                     /translation="MQSIFKKIFGKHTSQSAVKSDTVFSIEQDDDGWLYPQNATQLLN
                     TELRQKYLGLLWQQVSMNRQMFETFYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKARQSYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITLKD
                     GSRWFAWNGEPPEPYKFKYIKGRDYHLHLVIGSFFANQIIPKSAFDWLAQYPEVISSL
                     MYAMSGHNDKAGLLSEIVQKADQYSVTLALGGDVNKLVQRPVNSLSKQLVLALRYLLE
                     QKIKLNTPKGPADGWFTEEGLWLVSKTAADNIRTYLLGQGISVPKDNPKLFDEMQSLG
                     IVEESPSGGAIWSCRIQADSGWTPANVFTLLRIKPEVAWENINDRPNYFSGKVKIVEQ
                     IMEDSNMENHPPLVDESTKLVTLENVQPETIITSKEVEISQVEASSDNAPFSEIIIND
                     ENDLTSFVLDMFAPTESSEPSLADISIGKGQEIATMPSKQEVQDKPTTKKLNVTQSAV
                     ETPETLTLSELQSQNKENSITGDNFVNWLKSGIATNKFAINKPTAKLHIVEDHLFLVT
                     PVIFEMYLQESGLPYDKEMINSLQYDFQGLGIHLPKNTTINGKPDTINFWKCDVIGPR
                     KTSSLTGYLIKNTHLFFGDKTLLNNHCLTLQKEET"
BASE COUNT          707 a          478 c          464 g          815 t
ORIGIN      
        1 taaatgtctg acataattat tccatgttac agctttgatt ttatcgccaa caatttgatt
       61 acgccattca attagcatat cagtggtaat ctcatttgca tttaactttg ggtaacgtct
      121 tttgataatt cggattacat tattatagct ccgcttagta tcagggcgaa gatagtaacg
      181 atagaaaaaa aaatcggtct agtagcgaat caaaagagat agatgtcgtc attttaagtt
      241 tcctctttct gtaaagttaa acagtgatta tttaatagtg tcttatcccc aaagaagaga
      301 tgtgtatttt ttatcaaata tccagttaaa gaggaagtct ttctcggacc aattacatca
      361 catttccaaa aattgatggt atctggctta ccgtttattg tcgtattctt aggtaaatgg
      421 attcctagcc cttggaaatc atactgaaga ctatttatca tttccttatc ataaggcaga
      481 ccactctcct gcaaatacat ttcaaaaata acgggggtaa ctaaaaacag gtggtcttca
      541 acaatatgga gcttggcagt aggcttgtta attgcaaatt tgttggtcgc aatacctgat
      601 ttgagccaat ttacaaagtt atctcctgta attgagttct ccttattctg tgactgtagc
      661 tctgatagtg ttaatgtttc gggcgtttct actgcacttt gtgttacgtt gagcttttta
      721 gtggtaggtt tatcctgtac ttcttgtttg gatggcatcg ttgcaatttc ctgtccctta
      781 ccaattgaaa tatcagcaag gcttggctca gagctttccg taggtgcaaa catatccagc
      841 acaaaagagg ttaaatcatt ttcatcattt atgatgattt ctgagaacgg tgcattatct
      901 gagcttgcct ctacctgaga tatttcaact tccttagagg taataatagt ctctggttga
      961 acattctcta atgtaacaag tttagtgctt tcatcaacta atggaggatg attttccata
     1021 ttgctatctt ccattatctg ctcgactatt ttaactttcc cggaaaaata atttgggcga
     1081 tcattaatat tctcccaagc cacttcaggt ttaattctca ataatgtaaa gacgtttgcc
     1141 ggtgtccaac ctgaatcagc ttgaatacgg caactccaaa tcgccccacc agaaggagat
     1201 tcttctacga taccgagaga ttgcatttca tcaaataact ttggattatc cttaggcacg
     1261 gaaatcccct gccctagtaa gtaagttcgt atattatcag cggcagtttt gctaacaagc
     1321 cacaatcctt cctcggtaaa ccaaccgtct gcagggcctt tcggagtatt taacttgatt
     1381 ttctgttcca gtaaataacg tagtgccaag acaagctgct ttgacagaga attgacagga
     1441 cgttgaacca gtttatttac atcgccaccg agtgcgaggg taacactata ctgatcagct
     1501 ttttgcacga tttcagataa aagcccagct ttatcattat gtccggacat tgcatacatc
     1561 agagaagaaa tgacttctgg atactgtgcc agccaatcaa atgcagattt gggaataatc
     1621 tgatttgcaa agaagctgcc tatcacaaga tgaagatggt agtctcgacc tttgatgtat
     1681 ttgaatttat acggttcagg aggttctcca ttccaagcaa accaacgtga gccatctttg
     1741 agtgtgattt caatatctac tacaactttg ccaatatcgt gaactaatgc aagataaatt
     1801 gctgcggcag tccaagcgtc tcgctgcttt gactgttctt cgggagccac attcggtggc
     1861 aaaatataac tctggcgagc tttagctgaa aaagcaataa cttccaaacc gtgatcgagc
     1921 attccaccaa tatgagaatg atgatgtgat tctgaagctg gcaatagttg aaccatttca
     1981 gcgtaacgtt ctattggttg ctgataaaat gtctcgaaca tttgacggtt cattgatacc
     2041 tgttgccaaa gtagtccaag atacttttga cgtaattccg tattcagtaa ttgagtagca
     2101 ttttggggat atagccaacc atcgtcatct tgctcaatag agaaaacagt gtcagatttg
     2161 accgcacttt gagatgtgtg tttaccaaaa attttcttga aaattgattg cattgaaaga
     2221 gaccgaaaag tatcaatttt gtgttaaatg aaagccgttt agccctttgc ccttttgggc
     2281 aagacctttc cccttggaaa ggttaccttt caccctttag gggttatcct ttgcccttaa
     2341 ggactccttt atccttttgc catttagggt gagcttggaa tatattttgt agtgtataaa
     2401 tatatttctg ttaagttatc taaaaactct caaatatttt taaatagttt ttgtatcaat
     2461 ttac
//
LOCUS       DANLXS010000258         2444 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.266, whole genome shotgun sequence.
ACCESSION   DANLXS010000258 DANLXS010000000
VERSION     DANLXS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.266"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            10..834
                     /locus_tag="PXD71_002550"
     CDS             10..834
                     /locus_tag="PXD71_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL6292609.1"
                     /translation="MKSIKKLTLATLILVGLTACGSGGSGSSSNNAKSKPQVELKKTE
                     EDKKTEEDKKTEEDKKTEEDKKTEEDKKAEEAKKIAEAKGIGNKEYKDGLNELEEKNE
                     TGKNGEEIRTYGYLYNSHYSVVTAKMKQTLQENGRQMEPTVEVKGLKTENLPTEGKAT
                     YKGEAFDSHGNNSNSVVGGELIYNVDFSSRTGSGLVKNVQGGSIELAQGEIKNDSIIA
                     SAHQKYNDQAVGNGSYNIQFFGPNAEEIGGKIELNGEGEGSMKQMLGIAGTREEQK"
     gene            883..2277
                     /locus_tag="PXD71_002551"
     CDS             883..2277
                     /locus_tag="PXD71_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6292610.1"
                     /translation="MYKKHFLFLLVSPLVMAETVEFQQDPKEFDGQRQAVKFEQEIAK
                     KDLKLTAYLEEDNSLKGIEQHINQAILRQDWAKLERLLGDYAKQEQFDPLLLDYALAA
                     LAYAKKDYRSAAFYYQQLLKKQPDLTYPRFDLALILKEDYRYREAEQEFKQLKTDDLR
                     NAVNSALAQIEEEESWKPDFYLQYTQTNNVNNASSSPTVNVNGRIFIKESDSLPQSAK
                     GVRYGVGLSKMRNIGGNHFVGVDLGYNGVYYWNNHDYSEQSVSLTPKYSYRTATYRIG
                     VSPFIEQNWLGSSRYNYQFGSSINAVKLFERGLISGSFSHLQKRYSEQHIADRYNGYR
                     NQLTLGTQWQAVKNWRFFANLNASREITREKAQSSNKWLATIGVIYQTPSWASSVSVG
                     YGKRYFADKHYLFGYKRQDKEYQANVAVWNRSWHWQGIVPKLNFRYQKIDSNIADFYS
                     RSNKEVFMTLEKLF"
BASE COUNT          845 a          401 c          549 g          649 t
ORIGIN      
        1 ggagcaaata tgaagagtat caaaaaatta accctagcca ctttgatttt agtgggttta
       61 actgcttgtg gtagcggtgg ttcaggtagc agttctaata atgctaaatc gaaaccacaa
      121 gttgagttaa aaaaaactga agaggataaa aaaactgaag aggataaaaa aactgaagag
      181 gataaaaaaa ctgaagagga taaaaaaact gaagaggata aaaaggctga agaagcaaaa
      241 aaaattgcag aggcaaaagg aattggcaat aaagaatata aagatggatt aaatgagtta
      301 gaagaaaaaa atgaaacagg taaaaacgga gaagagattc gaacctatgg ttatctctat
      361 aacagccatt attccgttgt aactgccaaa atgaaacaaa ctttacagga aaatggtcgt
      421 caaatggaac ctacggttga agtgaaaggg cttaaaactg agaatttacc gacagagggc
      481 aaagcaacat ataaaggaga agcctttgat tctcacggta ataacagcaa ttcagtagta
      541 ggtggagaat taatctataa tgttgatttc tctagtcgta ccggatcagg tctagttaaa
      601 aatgtacagg gcggttctat cgagttagca caaggcgaaa taaaaaatga tagtattata
      661 gcttctgctc accaaaagta taatgatcag gctgttggaa atggtagtta caatatccaa
      721 ttctttggtc caaatgcaga agaaattgga gggaaaattg agcttaatgg tgagggcgaa
      781 ggctcaatga agcagatgtt aggtattgca ggaacccgag aagaacaaaa ataacattta
      841 atacccaatg ccgtttaatc ttaaacggca ttctttttta ctatgtataa aaaacatttt
      901 ctatttttac ttgtttcgcc tttagtaatg gcggaaacgg ttgagtttca gcaagatccc
      961 aaagagtttg atgggcagcg acaagcggtt aaatttgagc aagaaattgc aaaaaaggat
     1021 ttaaaattaa ccgcttactt ggaggaagat aattctctta aaggtattga acagcacatt
     1081 aaccaagcga ttcttagaca agattgggcg aagctggaaa ggctgcttgg cgattatgca
     1141 aagcaagagc aatttgatcc gctactttta gactatgctt tggcggcatt ggcttatgct
     1201 aaaaaagatt atcgctctgc ggcgttttat tatcagcagt tgctgaaaaa acagcctgat
     1261 ttaacctacc ctcgttttga tttggctctg attttaaaag aggattatcg ctacagggaa
     1321 gcagagcaag aatttaagca actcaaaacc gatgatttac gcaatgcggt gaacagtgcg
     1381 ttggctcaaa tagaggagga agagagctgg aagccggatt tttatttgca atatactcaa
     1441 accaacaatg tgaataatgc ttcttcgtcg ccaacggtaa atgtaaacgg gcggattttt
     1501 attaaagaat cagacagctt gccgcagtcg gcaaaaggcg taagatatgg cgttggcttg
     1561 agtaaaatgc gaaatatcgg cggtaatcat tttgtgggag tcgatcttgg ctataacggc
     1621 gtttattact ggaataacca cgattatagc gaacagtcgg tctcactaac accaaaatat
     1681 agctacagaa cggcaactta tcgtatcggg gtttcgccct ttattgagca gaattggctg
     1741 ggtagctcac ggtataacta tcaatttggt agcagtatca atgctgttaa actttttgaa
     1801 cgagggctga tttccggctc attcagccat ctgcaaaaac gttatagtga acagcatatt
     1861 gccgaccgtt acaatggtta tcgcaaccaa ctcacgctag gtacgcaatg gcaagcggtc
     1921 aaaaattggc gattttttgc aaatcttaac gctagccgtg aaattacccg tgagaaagct
     1981 caatcttcca ataagtggct ggcgaccatt ggcgtaattt accaaacccc aagctgggca
     2041 agttcggtaa gtgtcggata tggcaagcgt tattttgctg ataaacatta tctgtttggc
     2101 tataaacgcc aagataagga atatcaagcg aatgtggcgg tgtggaatcg aagttggcac
     2161 tggcaaggga ttgtgccgaa attgaatttc cgttatcaaa aaatcgatag caatattgcc
     2221 gatttctact ctcgtagcaa taaagaagtc tttatgacct tagagaaact attttaaggc
     2281 tgtgtatgaa tgtcggggag aaaaattttc tccccgatat ttatgtgtag aagatcagac
     2341 ttgatctgac aattcactct aaaaagtgag agtttcccgt ttagaatatg agtgtcaaaa
     2401 atcaatctaa acaaaaagga aactctcatg ttttattcta acaa
//
LOCUS       DANLXS010000259         2419 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.267, whole genome shotgun sequence.
ACCESSION   DANLXS010000259 DANLXS010000000
VERSION     DANLXS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.267"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            8..856
                     /locus_tag="PXD71_002552"
     CDS             8..856
                     /locus_tag="PXD71_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL6292611.1"
                     /translation="MEKKALFRGFLLSTVALAVAACGNLSKVSDEGTTENPVFPKISE
                     SEFNHDGSQFGSWPNWENVRQIEKGMNKDQLYNLIGRPHFEEGLYGVREWDYAFNYRE
                     NGVHKICQFKILFDKNMNAQSFFWYPNGCNGNSSFALTGDFLFDFDKATLTTKGKEVV
                     DNVAQQLKASKAQQVKVAGYTDRLGSAAYNLDLSQRRSNTVKARLVQQGVTAQIEAVG
                     YGKANQVKACDGETGQALKDCLRPNRRVEISANCGVMKQNEGGNVAGPNGPAPLYQTP
                     AYDGSK"
     gene            944..1762
                     /locus_tag="PXD71_002553"
     CDS             944..1762
                     /locus_tag="PXD71_002553"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HDL6292612.1"
                     /translation="MNYQAIAFDLDGTLLSSNATILESSKEAIKKVREKGIKVYFVTG
                     RHHTAVRPYYVELGLDTPVVCCNGTYVYDFKNEKVLSGNPLSAEIASKLVNDAQAQGI
                     HTAVYFEDAMTYESLNIHFTKFQKWVESCAPEVRPNVYQVENFQQEIDKGVTVWKVLI
                     SDPDLAKMQKFVEKLPPDQVSPEWSWVDRVDITSVGNSKGARLAEVLKTEGIDLQKVI
                     AFGDNFNDVSMLETVGLGIAMGNSEPDIQQKAKKTIGSNNEDSIANELKAQFNL"
     gene            1819..>2419
                     /locus_tag="PXD71_002554"
     CDS             1819..>2419
                     /locus_tag="PXD71_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HDL6292613.1"
                     /translation="MIGFASIISGISLGIMWSNKSDASLINVSGSLRMQSYRLLSEMD
                     SKKALLPERLLEYHRTLHATELSSLKQSVLLPDSVIESYQQLLKDWYEMQSYVAQGDK
                     VNYASHIEDYVQRVDDFVFRLQEFAELKLKIATGVIAIAMLLIIALAYIGVWYTRKKI
                     IGPLRQLVKASWQIQNKDFDHVKLAVNEPNELGLLSSTFT"
BASE COUNT          804 a          373 c          503 g          739 t
ORIGIN      
        1 tatgaaaatg gaaaaaaaag cccttttccg tggattttta ttatcgacag tggcattagc
       61 agtggctgct tgtggtaact taagtaaagt gagtgatgaa ggaacaactg aaaacccggt
      121 attcccgaaa atctcagaat ctgaatttaa ccacgatggt tcacaatttg gttcatggcc
      181 aaactgggaa aacgttcgtc aaattgaaaa agggatgaac aaagatcaat tatataatct
      241 aattggtcgt cctcattttg aagaaggctt atatggtgtt cgtgaatggg actatgcatt
      301 caattaccgt gaaaatggcg tacataaaat ttgccaattt aaaattttat tcgataaaaa
      361 tatgaatgca caaagtttct tctggtatcc aaatggatgt aatggaaact catcatttgc
      421 tttaactggc gatttcttat tcgatttcga taaagcgact ttaactacaa aaggtaaaga
      481 agttgttgat aatgttgctc aacaattaaa agcatctaaa gcacaacaag ttaaagtagc
      541 aggctataca gaccgtttag gctctgctgc ttataactta gacttatcac agcgtcgttc
      601 taatacagtt aaagctcgtt tagtacaaca aggcgttaca gcacaaattg aagcggtagg
      661 ttatggtaaa gcgaatcaag taaaagcttg tgatggtgaa actggtcaag cattaaaaga
      721 ttgtttacgc cctaaccgtc gtgttgaaat ttctgcaaat tgtggtgtaa tgaaacaaaa
      781 tgaaggtggc aatgttgcag gtcctaatgg tccggctcca ctttatcaaa ccccagcata
      841 tgatggcagt aaataatttc tagttttgac taacttagag tagaatatta aggcaaccaa
      901 atggttgcct tttcttttat ctatttattt ataggggaat actatgaact accaagctat
      961 tgcatttgat ttagacggaa cactactttc atcgaacgca acaattttag aatccagtaa
     1021 agaggctatt aaaaaggtaa gagaaaaggg aattaaagtc tactttgtta ccggtaggca
     1081 ccatactgcg gttcgccctt attatgtaga actagggctg gatacgccag ttgtgtgttg
     1141 caatggcact tatgtgtatg attttaaaaa tgaaaaagta ttaagcggta atccgttatc
     1201 agcagaaatt gcttcaaaat tagttaatga tgcacaagct caagggattc atactgcggt
     1261 ttattttgaa gatgcaatga cttatgagtc tttgaatatt cacttcacta aattccaaaa
     1321 atgggttgag tcttgcgcac cagaagttcg tccgaatgtt tatcaggtag aaaatttcca
     1381 acaagaaatt gataaaggtg taaccgtatg gaaagtgctt attagcgatc cagatcttgc
     1441 caaaatgcaa aaatttgttg aaaaattacc gcctgatcaa gtaagcccgg aatggtcttg
     1501 ggttgatagg gttgatatta ccagtgttgg taacagcaaa ggggcaagat tagcggaagt
     1561 attaaaaaca gaaggtattg atttacaaaa ggtgattgcc tttggcgata atttcaatga
     1621 tgtttctatg ctagaaaccg ttggattagg cattgcaatg ggaaattcag aaccagatat
     1681 tcagcaaaaa gcgaagaaaa ctataggttc aaataatgaa gatagtattg ctaatgaatt
     1741 gaaagctcaa tttaatcttt aattatgata aaagcaaaat actctattgc taggcggata
     1801 ggactctatt ttttaattat gatcggattt gcttcgatta tcagtggaat ctcacttggt
     1861 attatgtgga gcaataaatc tgatgcgagc ctgattaatg tttcaggctc acttcgtatg
     1921 caaagttatc gattgttatc agaaatggat tcaaaaaaag cgctgctacc tgagcgattg
     1981 ttggaatatc atcgaacgct acatgctaca gagcttagtt cattaaagca aagtgtttta
     2041 ttgccagata gcgtgataga aagctatcaa caattactta aagattggta tgaaatgcaa
     2101 tcttatgtcg ctcagggaga taaagttaat tatgcttcgc acattgaaga ttatgtgcag
     2161 agagttgatg attttgtttt ccgcttacaa gagttcgctg agttaaaatt aaagattgca
     2221 acaggcgtga ttgcgattgc aatgttactg attattgccc ttgcttatat tggcgtttgg
     2281 tatactcgaa aaaagattat tggaccttta aggcaattgg taaaagcgag ttggcaaatt
     2341 caaaataaag attttgacca cgtaaaatta gcggttaatg aacccaatga attagggcta
     2401 ctttcctcta cttttactg
//
LOCUS       DANLXS010000260         2405 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.268, whole genome shotgun sequence.
ACCESSION   DANLXS010000260 DANLXS010000000
VERSION     DANLXS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.268"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            184..402
                     /locus_tag="PXD71_002555"
                     /pseudo
     CDS             184..402
                     /locus_tag="PXD71_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            complement(454..669)
                     /locus_tag="PXD71_002556"
     CDS             complement(454..669)
                     /locus_tag="PXD71_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292614.1"
                     /translation="MQTEKRGRGRPKSGLTLQELQAKSDAKRGVRLKSFKFHEDFIAQ
                     LEQLAEQHGISQTQVIVQAVEQFSKGA"
     gene            1214..1915
                     /locus_tag="PXD71_002557"
     CDS             1214..1915
                     /locus_tag="PXD71_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292615.1"
                     /translation="MSTQISTQTISFNNQALITFEQNGTHYTAMKPICENIGLTWHAQ
                     YERIKRDEILSQGILIIRIPTNGGDQQMICLPIEYLNGWLFGIDINRCKPEIRETLIK
                     YKKECYQALHDYWFKGKAERSQIAPIVEPKIPMELTKKEWLRFASMWYALYNSLEVLA
                     TLEKPLRAIGSPFGATAYTHATEYQTTLGVMKRILEPMLADFEVDPFEEAHYHKALTT
                     LRQYQPKGLGGLVRI"
     gene            2169..2345
                     /locus_tag="PXD71_002558"
     CDS             2169..2345
                     /locus_tag="PXD71_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292616.1"
                     /translation="MTEEDKEYLQTKIENEGFEYAFVSYSDFEEVQDEKFHGLRKAYL
                     KARSELAEYIDIED"
BASE COUNT          758 a          543 c          455 g          649 t
ORIGIN      
        1 ccttatattt ttttatgaga ttactacgaa agcgttttac tgtcagaaca aaaatttatt
       61 ttttcgatgc agatcgcaaa aatagatatt gaggcttgac accgccaaac ttcggattaa
      121 gataaccgca ctttcaagcc gtccaccaac ggcttttttt gtacctaaaa acggagaaac
      181 gaaatggcaa atagcgtaat gattgtacaa ggcgtggagg tcaaagtaac cgctcgtgat
      241 ggtgaggatt acatcagcct aactgatatg tgcaaagctt ttggagacag cgaccagctc
      301 attaaaaact ggcttcaaaa taaaaatacc attgaatttc ttcaagtttg ggaagagctg
      361 aataatccaa attttaattt ggtggaatta caccaaatta aatgcaacaa ctaattaaaa
      421 acatcgaaca gtgaagggta ggtttatagg ggcttacgcc ccttttgaaa actgctcaac
      481 cgcttgaacg attacctgtg tttgcgaaat gccgtgttgt tctgcaagct gttcaagttg
      541 agcaataaaa tcttcgtgga acttgaatga cttcaatcgc acaccacgct ttgcatcgct
      601 ttttgcttgt aattcttgaa gcgttaaacc tgatttaggg cgaccacgac ctcgtttctc
      661 tgtttgcatt gtcaattcct tgttgatttt taatacggaa cgttatatta tttaggaact
      721 gcctagcaga gatggcgttc tctgctagga cttttagctt tctaatctaa taagctggcg
      781 tgcttattaa gattaagatc actaggatta acaacttaag atacataatc cagttcctga
      841 tgtttgccgc tctcttcaag ggcggcttct tcattttcag accctttccg aaaacaagat
      901 tattttaata cataccaaat aaaacgcaag ctatttttta gtatttatta aaataatttt
      961 atttgacaac cgccaaactt taacttacta ttccgcccaa ggtgtcgaaa cctcatactc
     1021 taaggcggat agttcactaa tcgccactag gcgatttttt tatatccgta atcctgacta
     1081 tgtcgggagg gcgactaata caagaccgaa aggaaatacg tccagccctt accttagagt
     1141 gggttttcga acctcccgac gccactgtcg aaagtggctt gttttaaaca aatagactct
     1201 aaggattacg actatgtcaa ctcaaatttc tactcaaacc atttctttca acaaccaagc
     1261 cttaatcaca ttcgagcaaa acggcacaca ttacaccgca atgaaaccga tctgcgaaaa
     1321 tatcggcttg acttggcacg ctcaatacga acggatcaaa cgagatgaaa tcctttcgca
     1381 gggtattctt atcataagaa tacctacaaa cggtggcgat caacaaatga tctgcctccc
     1441 aattgaatac ctcaacggct ggctcttcgg cattgacatc aatcgctgca aacccgaaat
     1501 cagagaaacg ctcatcaaat acaaaaaaga gtgctaccaa gccctgcacg attactggtt
     1561 caagggcaaa gcggaacgtt cacaaatcgc cccgattgtc gaacctaaaa tcccaatgga
     1621 gctaaccaaa aaagaatggc tacgtttcgc ttcaatgtgg tacgccctct acaacagcct
     1681 tgaagtactt gctacgttag aaaaaccgtt gcgtgctatc ggttcgccgt ttggggcaac
     1741 cgcctacact cacgccaccg agtatcaaac cacgcttggc gtaatgaaac ggatacttga
     1801 accgatgtta gccgacttcg aggtcgatcc gtttgaagaa gctcactacc acaaagccct
     1861 aactacctta aggcaatacc agccgaaagg cttaggcggc ttagtccgaa tttaatttgc
     1921 aaaaaaatca cacaatccga ccgcttgtga aacatcaagc ggcggtttcc tgcacccaaa
     1981 attcaaggat taggcgatga aatacgcaaa aatcatactc tttttaaccg cctttgcagt
     2041 tgctgccgac tacttagagt tacacaacga ctgcgatggc aaaccatgca ctatcgcaca
     2101 gcgataatgc ttccccgacc ctgcccgtgc agtgtttatt tttatccact ctcaatagga
     2161 gaaacgaaat gacagaagaa gacaaagaat atcttcaaac taaaattgaa aacgaaggtt
     2221 ttgaatacgc tttcgtcagt tatagtgact ttgaagaagt ccaagatgaa aaattccacg
     2281 ggcttcgcaa ggcttattta aaagctcgaa gtgagttagc ggaatatatt gatattgaag
     2341 attaaccact gatgaactgt tcattgaaca aatgaacact tgacaaccaa ccgcttgtag
     2401 ctaac
//
LOCUS       DANLXS010000261         2404 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.269, whole genome shotgun sequence.
ACCESSION   DANLXS010000261 DANLXS010000000
VERSION     DANLXS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.269"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..338
                     /locus_tag="PXD71_002559"
     CDS             <1..338
                     /locus_tag="PXD71_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL6292617.1"
                     /translation="CDELVTINEKIVNLTYGDQDALNIYFKGGWGALPIEYNYQVDAI
                     LELVLRREQEELARKNGYLDVIPKIIHYTSKFKPWKKELHTMQISTRKKYWFYYHLEW
                     NDILEQHQK"
     gene            complement(384..671)
                     /locus_tag="PXD71_002560"
     CDS             complement(384..671)
                     /locus_tag="PXD71_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL6292618.1"
                     /translation="MKNDKPIECEGCNTFDVGSILDNSETVATIQRTYESENEAKAAL
                     EKFTQKARDVESEPCEISSEITPIESGFELVAHFKFSCQAEVVIFQLGTRF"
     gene            791..1204
                     /locus_tag="PXD71_002561"
     CDS             791..1204
                     /locus_tag="PXD71_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HDL6292619.1"
                     /translation="MTAWERVFHALLFECFAIIFTVILTSWLTSHRMVDLTAVIVMIS
                     VIAMVWNVIFNWGFDKVFTGERVQRGLGIRILHSFLFEGGLLVFTIPLVMYMLDIGWW
                     QAFVMDIGMTLFVLVYSVVFNWVYDHLRLRWVKSG"
     gene            1289..2281
                     /gene="mglB"
                     /locus_tag="PXD71_002562"
     CDS             1289..2281
                     /gene="mglB"
                     /locus_tag="PXD71_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL6292620.1"
                     /translation="MKRTILSAFALSVICGVNLSYAQSESRVGVTIYKYDDHFMTQMR
                     KDMQNEAKGFAGLKWFMNDAQNSQVKQLAQIETLLQHKMRVLAVNIVNANESKTIVEK
                     AKAYNVPVVFFNRTPSKQALESYEKAYFVSADPKEAGRIQGELIAKVWKSNPDFDLNK
                     DGKLQFVLLKGEPSDVSAERSQAVVESLNQQGVQAEEIYSDTARWRRTLARNKMNEWL
                     VENRAAEIEVVISNNDEMAIGALDALNAHEKRLPIFGIDALPETLALMKKGEIAGTVL
                     NDSSAQAKAVVKLVSNLAQGKPALKDTQWESHSSKTIYLPHIGIDKENLGQFLP"
BASE COUNT          716 a          378 c          557 g          753 t
ORIGIN      
        1 tatgtgatga gttggttact atcaatgaaa aaattgtaaa cttaacttat ggcgatcaag
       61 atgctctcaa tatttacttt aaaggcggtt ggggagcatt gccgatagag tataattatc
      121 aagttgatgc aattcttgaa ctagtattaa gaagagaaca agaggaactt gcgagaaaaa
      181 atggctattt agatgtgatt ccgaaaatta tccactatac gagtaaattc aaaccttgga
      241 aaaaagaact gcatacaatg caaatatcta cgcgtaaaaa atattggttt tactatcatt
      301 tagagtggaa cgatattttg gaacaacatc aaaaatagct atcatacaaa agctgattca
      361 agtgtttgaa tcagcttttt gtattagaaa cgtgttccta attgaaaaat aaccacttct
      421 gcttggcagc taaatttgaa gtgagcaacc agttcaaaac cgctttcaat cggggtgatt
      481 tcactgctga tttcacaggg ttcagattcg acatcacggg ctttttgagt gaatttttca
      541 agtgccgctt tagcttcatt ttcgctttca taagtgcgct gaatagtagc gactgtttcg
      601 ctgttatcta aaatagaacc tacatcaaat gtgttacagc cttcgcattc aatcggttta
      661 tcgtttttca ttggctaatc cttctaaatt gatgaataat gtaggtactt taaaactaaa
      721 ttgcgtaacc tgcaatcatc agtgaacgat tatttgattt agttggtatt ttgcaaagtt
      781 tggaggaaaa atgaccgctt gggagcgtgt gtttcacgcg ctgttatttg aatgtttcgc
      841 tattattttt acggttatct taacttcttg gctgacttct catcgtatgg ttgatttaac
      901 cgcagtgatt gtgatgattt cggtgattgc aatggtgtgg aatgttattt ttaactgggg
      961 ttttgataag gtgtttaccg gtgagcgagt gcaacgtggt ttaggcattc gtattttgca
     1021 ttcattcctt tttgagggcg gtttgctggt ttttactatc ccattggtaa tgtatatgct
     1081 cgatatcggc tggtggcagg cgtttgtgat ggatattgga atgaccttat ttgtattagt
     1141 ttattcggta gtttttaatt gggtgtacga tcatttacgc ctgaggtggg ttaaatctgg
     1201 ctaaattgtg aactagatca cggtttcctt acacaaaatt cgctatgctt gaattggttc
     1261 atatttggca tagcaagagg gacattctat gaagcgtaca atcttgagtg cttttgcact
     1321 ttctgttatt tgcggtgtga atctaagtta cgcacaatct gaaagcagag tgggggtaac
     1381 gatttacaaa tatgatgacc attttatgac tcaaatgcgt aaggatatgc aaaacgaggc
     1441 gaaaggcttc gctgggttga agtggtttat gaatgatgca caaaattccc aagtgaagca
     1501 attagcgcaa attgaaacct tacttcagca taaaatgcgt gtgttggcgg tgaatattgt
     1561 gaatgcaaat gagtcgaaaa ctattgtgga aaaagcgaag gcttataatg tgccggttgt
     1621 cttttttaac cgcacaccga gtaaacaagc gttagaaagt tacgaaaagg cgtactttgt
     1681 gagcgctgat cctaaagagg ccggtagaat ccaaggggaa ttaattgcga aagtgtggaa
     1741 atcaaacccc gatttcgatc taaataaaga cgggaaattg caatttgtat tgcttaaggg
     1801 cgagccgagt gatgtgagcg ctgagcgttc tcaagctgtg gttgaatcgc ttaatcaaca
     1861 aggtgttcag gcagaagaaa tttacagtga cacggcgaga tggcgtagaa cattggctcg
     1921 caataaaatg aatgagtggc tggtggaaaa ccgtgcagct gagattgaag ttgtgatttc
     1981 aaacaatgat gaaatggcaa ttggtgcttt agatgcgtta aatgcacacg agaaaagatt
     2041 gccgatcttt ggtattgatg ccttgcctga gacgttagca ttaatgaaaa aaggggaaat
     2101 agccggcacg gtattaaatg atagctctgc acaagcgaaa gcggtggtga aattggtatc
     2161 taatttggca caaggtaagc ctgcgctgaa agatacgcag tgggaaagcc attcctctaa
     2221 aacgatttat cttccacata ttggtattga taaggaaaat ttagggcaat ttttacctta
     2281 aatggaggag cggggcgtta taaatacgcc ccttattgat gataacaagc ggtcattttt
     2341 cagtaaaatc ttgcaaatcc tttctctttt cttccaacag atttgaatag agcgaatatt
     2401 caaa
//
LOCUS       DANLXS010000262         2384 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.270, whole genome shotgun sequence.
ACCESSION   DANLXS010000262 DANLXS010000000
VERSION     DANLXS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.270"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..168
                     /locus_tag="PXD71_002563"
     CDS             <1..168
                     /locus_tag="PXD71_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292621.1"
                     /translation="THATEYQTTLGVMKRILEPMLADFEVDPFEEAHYHKALTTLRQY
                     QPKGLGGLVRI"
     gene            404..895
                     /locus_tag="PXD71_002564"
     CDS             404..895
                     /locus_tag="PXD71_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6292622.1"
                     /translation="MQTKPILDACCGSRMFHFDKQNPLVLFADNRQIETTMKDRDKIR
                     HLEIKPDVLHDFTNMPYENESFWHIVFDPPHLVQGGENSWLVKKYGKLPCNWREVLKK
                     GFDECMRVLKPNGTLIFKWNETQIPVSEILKVIDCQPIYGHKSGRLNQTHWLAFVKIE
                     QSL"
     gene            922..1179
                     /locus_tag="PXD71_002565"
     CDS             922..1179
                     /locus_tag="PXD71_002565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292623.1"
                     /translation="MEQIKLSEKAERALILSQLNENSAMIIDLDDVALILNCGYDYVQ
                     SKISKKPGFPEPVQGINGKSVLFKSGDVIRWVNRQARTRKN"
     gene            complement(1176..2165)
                     /locus_tag="PXD71_002566"
     CDS             complement(1176..2165)
                     /locus_tag="PXD71_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL6292624.1"
                     /translation="MASIRKRGSKYQVEISKKGVRKSATFRTKAEAQAWAYEEERKIE
                     IISKGGVVDILFAEALERYQNEVSINKRTWRREIGRINNILKEPIANRYVQDIKRDDI
                     EDWIEARLKRGLKGSSVERDLSILSNVFTYCLKRWGYISENPMIGVIHPNKVPPRKQR
                     YSQEDIDKILEVSKYREDIKRSNARLAAAMLFAIETAMRAGEISGLTWDNVNFEKRIA
                     HLPMTKNGSSRDVPLSSKAIEILKRLETIKKDNSVFQLPQGNGLSARFFELKEKAGLS
                     YLNFHDTRREALSRLAKKVDVMTLAKISGHKDVKILLNTYYAPNMEEVAHLLD"
BASE COUNT          738 a          523 c          435 g          688 t
ORIGIN      
        1 acgcacgcta ccgagtatca aaccacgctt ggcgtaatga agcggatact tgaaccgatg
       61 ttagccgact tcgaggtcga tccgtttgaa gaggctcact accacaaagc cctaactacc
      121 ttaaggcaat accagccgaa aggcttaggc ggcttagtcc gaatttaatt tgcaaaaaaa
      181 tcacacaatc cgaccgcttg tgaaacaaca agcggcggat ttccataccc aaattttgag
      241 gacaagccga tgaaatacgc aaaaatcatt ctatttttaa ccgcctttgc agttgctgct
      301 gaccatctag agttacgcaa cgactgcgat ggcaaaattt gtatggctca acgctaatta
      361 acccaaccgc ttgttccgca agcggttatt ttttggagga aaaatgcaaa caaaaccgat
      421 attagatgcg tgttgtggtt cccgtatgtt tcattttgac aagcaaaatc cactcgtcct
      481 gtttgctgat aatcgacaaa tagaaaccac aatgaaagac cgagacaaaa tccgccattt
      541 agaaatcaaa cctgatgttt tacacgattt cacgaatatg ccatacgaaa acgagagttt
      601 ttggcacatt gtttttgatc cgccacattt agttcaaggt ggagaaaata gctggcttgt
      661 aaagaaatac ggcaaattac cttgcaactg gcgagaagtc ttgaagaaag gctttgacga
      721 atgtatgcga gtgctaaaac caaacggcac gcttattttc aagtggaatg aaacacaaat
      781 ccccgtttca gaaattttaa aagttattga ttgtcagccg atttatggac acaaaagcgg
      841 acgcttaaac caaacacatt ggctggcgtt tgtgaaaatt gagcaatccc tctaaatgag
      901 gggtttattt ttggagagaa tatggaacaa attaaacttt cagaaaaagc ggagagagcc
      961 ttaattcttt ctcagctcaa cgaaaattca gcaatgatta ttgatttaga tgatgttgcc
     1021 ctgattttga attgcggata tgactatgtg caatccaaga tcagtaaaaa gcccggtttc
     1081 ccagagccag tgcaaggcat taacgggaaa tcagttcttt tcaaaagtgg cgatgttatc
     1141 cgttgggtca accgtcaagc acgaacacgc aaaaattaat ccagcaaatg agcgacttct
     1201 tccatatttg gggcgtagta agtgttaagc aaaatcttca catctttatg ccctgaaatt
     1261 ttggctaagg tcatcacatc gacttttttt gccaagcgtg aaagagcctc tctcctcgtg
     1321 tcgtgaaaat tcaaataaga aagcccagcc ttttctttta actcaaagaa tctagcactt
     1381 aaaccattcc cttgtggaag ctgaaaaacg gaattatcct ttttgatggt ttccagtctt
     1441 ttcaagatct cgattgcctt tgatgataac ggaacgtctc ttgatgaacc gttttttgtc
     1501 ataggcaaat gagctattct tttctcaaaa ttcacattat cccacgttaa acccgaaatc
     1561 tcaccggcac gcattgccgt ttcaatcgca aataacatag ccgcagccaa tcgagcatta
     1621 gagcgtttaa tatcttctcg gtatttgctg acttctaaga ttttatcaat atcttcctgg
     1681 gaataacgct gttttcttgg tggcactttg tttgggtgaa taacaccgat catcggattt
     1741 tcactaatat agccccagcg ttttaagcag taggtaaata cattgcttaa aatagacaaa
     1801 tccctctcaa cacttgagcc ttttaaccct ctcttcaatc tagcttcgat ccaatcttca
     1861 atatcatctc gcttaatatc ttgtacataa cgattcgcaa taggctcttt caatatgtta
     1921 ttgattcgac ctatctctcg tcgccacgtc ctcttattaa tcgatacttc attttgatac
     1981 cgctctaacg cttccgcaaa taaaatatca actaccccac ctttcgagat gatctctatt
     2041 ttacgctctt cttcgtaagc ccatgcctgt gcctctgctt ttgtgcggaa ggtagctgat
     2101 tttctcaccc ctttcttcga gatttcgact tgatatttac tgccccgctt gcgaatgctc
     2161 gccatatttt tacctgcctt ttgtcgtaaa tttgtcgtaa tttataccat acaataaccg
     2221 acaataacag ataataaccg attttatgaa atcatcttaa ggcaaagaag agaggaaaaa
     2281 cttaagataa cctattgaaa taactataat aaacgataat aaccgacaaa aaacggaaat
     2341 aaaaaaaagc ccctaatcaa aaaagcgaaa aggggcggaa gtct
//
LOCUS       DANLXS010000263         2363 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.271, whole genome shotgun sequence.
ACCESSION   DANLXS010000263 DANLXS010000000
VERSION     DANLXS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.271"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..186
                     /locus_tag="PXD71_002567"
     CDS             <1..186
                     /locus_tag="PXD71_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HDL6292625.1"
                     /translation="LLPVVIEMLVSRKLIPDLNALFGQLPESMWYFLNIFQIPMAISL
                     MISAMISRFVRRMPFIG"
     gene            196..735
                     /locus_tag="PXD71_002568"
     CDS             196..735
                     /locus_tag="PXD71_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292626.1"
                     /translation="MAVSAYIGIPGSGKSYEVVKSVILPAVASNRRVVSNIYGLNKEK
                     IHQYLLRKNKKLMIEELGEVIYVTNEQCLESDFLPSMDEQDSFCKAGDLIIIDEVWRI
                     WGNDKDIPKNHRSFIAEHRHFADPITGVTCDLVVINQDISQIPRFIKEGLRQLTECKN
                     TLVWGCVIVIGLMFFKERI"
     gene            666..1229
                     /locus_tag="PXD71_002569"
     CDS             666..1229
                     /locus_tag="PXD71_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292627.1"
                     /translation="MQKHVGLGLRNRYRVDVFQGKNLAKKNRTNSYQEKYDKKIFELY
                     KSYESDGAREAVTDSRQSIFSSFWVKVWIILLPLALFYCGYNLYIFLNGSESEVSHNT
                     EQAKFASDENHNDIVIHLLPTIGKPLSTKWRITGELKRDNKSFVILADNENRLRLEPS
                     SQFSFSGRMLQGEIDGGIVSYFSGESK"
     gene            1226..2359
                     /locus_tag="PXD71_002570"
     CDS             1226..2359
                     /locus_tag="PXD71_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HDL6292628.1"
                     /translation="MKIHQLGLLLMISSFSYAKNVDFKLEAVPLPKAISIIYDEVLQK
                     PYMLDPKLSNDSRVISFHTTEQQNFEQFIQRYFSNMNISVSEKKGVVYIGHIEPKQPK
                     VIKRSFVYSPIHRDVDYLASFLKDGTEGNQVFASGDKLVYYGTSEDIARVKSILKSID
                     TKSREVVVTGYVFEVQNIEKEGSGINLLAKLLSGKLGISVGYKQSYENFITVNAGNLD
                     AMFELFRTDQRFRVVSSPTLRVKSGSRGNFSVGSDVPVLSNVTYQDGRPIQSIEYRSS
                     GVIFDIQPTIKDKAIDLKIQQLSNFIKTDTGVNNSPTLVKRDLVTDVTVKSGDVIVLG
                     GLAENKISNGETSFSLLPKGWLSGKSNENTKTDIIVLLQVKEI"
BASE COUNT          801 a          323 c          485 g          754 t
ORIGIN      
        1 ctacttcctg tagtaataga gatgcttgtg tctagaaaat tgatacctga tttaaatgcg
       61 ttatttgggc aattgcctga aagtatgtgg tattttttaa atatttttca gataccaatg
      121 gctatttctt taatgatttc agcaatgatt tcacgttttg ttagacgtat gccatttatt
      181 ggatagagga gtgctatggc tgtttcagcg tatataggga ttcctggaag cggcaaatcg
      241 tatgaagtgg ttaagtcagt aattttacct gctgttgctt cgaatcgaag agttgtttcc
      301 aacatttatg gattaaataa agagaaaatt catcaatatt tactgcgtaa aaataaaaaa
      361 ttaatgattg aagagcttgg tgaagtcata tatgtaacga atgaacaatg cttggaatcg
      421 gattttttac catcaatgga cgaacaagat tcattttgca aagctgggga cttgataatt
      481 attgatgaag tttggcgtat ttggggaaac gacaaggata ttccaaaaaa tcatcgttca
      541 tttattgctg aacatcgaca ttttgcagat cctataacag gtgtaacttg tgatttagtc
      601 gtaataaatc aagatatttc tcaaattcca agatttatta aagaaggatt gagacaactt
      661 accgaatgca aaaacacgtt ggtttggggt tgcgtaatcg ttatcgggtt gatgtttttc
      721 aaggaaagaa tttagctaaa aagaaccgga caaattctta tcaagaaaaa tatgataaaa
      781 aaatatttga gctttataaa tcttatgaat ctgatggtgc aagagaagcg gttacagata
      841 gtaggcaatc tatattttca tctttttggg tcaaggtatg gattattctt ttaccattgg
      901 cattatttta ttgtggctat aacctttaca ttttcctaaa tggttcagag agtgaagtaa
      961 gccataatac tgaacaggct aaatttgctt ctgatgaaaa ccataacgat atagtaattc
     1021 atttacttcc tactattgga aaacctttat caactaaatg gcgaataaca ggggaactaa
     1081 aaagggataa taaatcattt gtgatcttag ctgacaatga aaaccgttta agacttgagc
     1141 ctagtagcca gtttagtttt agcggtcgaa tgttgcaagg agagatagat ggtggaatag
     1201 ttagttattt ttcgggagaa tctaaatgaa aatccatcaa ttagggctat tgctcatgat
     1261 tagctcattt tcttatgcta agaatgttga ttttaaatta gaagctgtgc cgttaccgaa
     1321 agcaatatca ataatatacg atgaagtgct acaaaagcca tatatgcttg atcctaaatt
     1381 atctaatgat tcaagggtta ttagctttca tacaacagag caacagaatt ttgagcagtt
     1441 tattcaacgg tatttcagca atatgaatat cagtgtatca gagaaaaaag gtgttgttta
     1501 tattggacat attgaaccaa aacagccgaa agtaataaaa cgtagttttg tgtattcgcc
     1561 tattcatcgt gatgttgatt atttggctag cttcctgaaa gatggaacag aaggaaacca
     1621 agtttttgca agtggcgaca agcttgtata ttatggtact tctgaagata ttgcacgagt
     1681 gaaaagtatc ttgaaatcaa tcgacacaaa aagccgtgaa gtagtggtaa ctggttatgt
     1741 ttttgaagtg cagaatattg aaaaagaggg tagtggcata aacttgttag cgaaattgct
     1801 atcaggaaaa ttaggcatta gtgttggtta caagcaaagt tatgaaaact ttatcaccgt
     1861 gaatgcagga aatttagatg caatgtttga attgtttcgc acagatcagc gttttagggt
     1921 tgtcagcagt ccgacattaa gagtaaaatc aggctcaaga ggtaattttt cagtaggctc
     1981 tgatgtccct gtattatcga atgtaaccta tcaggacgga aggcctattc agtcgattga
     2041 ataccgttca agcggtgtaa tatttgatat tcagccaacg ataaaagata aagcgattga
     2101 tttaaaaatt caacaactat caaatttcat caaaacagat accggtgtaa ataatagccc
     2161 aacgttggta aagcgtgatc tggtaacaga tgttacggtt aaaagcggtg atgttatcgt
     2221 tttaggtggg ttagctgaaa ataaaatatc taatggcgaa accagttttt cattgctacc
     2281 taaaggttgg ttaagtggaa aatcaaatga aaatactaaa acagatatta tagttctgct
     2341 acaagtaaag gaaatttaat atg
//
LOCUS       DANLXS010000264         2339 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.272, whole genome shotgun sequence.
ACCESSION   DANLXS010000264 DANLXS010000000
VERSION     DANLXS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.272"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            80..289
                     /locus_tag="PXD71_002571"
     CDS             80..289
                     /locus_tag="PXD71_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HDL6292629.1"
                     /translation="MSKQKNDYLHQRGQIKESAVKALVTDPLFRQRVERNRKGKGSYR
                     RQEKHKKTIYGQKETPFKNIRLESF"
     gene            complement(338..1060)
                     /gene="bioD"
                     /locus_tag="PXD71_002572"
     CDS             complement(338..1060)
                     /gene="bioD"
                     /locus_tag="PXD71_002572"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDL6292630.1"
                     /translation="MPSLFITGTDTNVGKTVVTRAIIQTLTKHSFPVIGYKPIACGGD
                     DSLPTEPTQYDYATEDNSDVLTILDSCPTNVSYRDINSYTFIHSSTPVFAALDAVHHI
                     QLEKLNTDLKRLENAYPNVVVEGTYGWLTPINKDYYFADWVMQNNMPVVLVVGIKEGC
                     VNHALLTANEILQRGVKLVSWVANRVNPGLRHYHELIELLTQKINAPLLGQIPYMGHP
                     EHKELSSYIQNPEPLLAYFSKP"
     gene            complement(1108..2295)
                     /locus_tag="PXD71_002573"
     CDS             complement(1108..2295)
                     /locus_tag="PXD71_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL6292631.1"
                     /translation="MFSDNKALHLGKIYHLIEQFELISRTDLAKLSGLAPASVTNLTK
                     ILIDNHFILERTVQTTLSRGRPSVGLAVSNFFWQLLCVTVSSHKIEISLCKLNGAEIY
                     KQDYTISAENYPRLNDFILNFITNFRQLYCVNTSQLLAVSISVIGKINAEKDSITQLG
                     NKNIFCNITSKLSPLFHCPILLSEHFQLWLLAESTVGSLINQDNAIFLQLDDTVNLSV
                     LLSGSLLSKQSKMNVDNMLMPRFSHLSDEIFPELDEIHRYKLVNQVTFPAIVRLIDRY
                     LPNNLKSQEQKISWLCQKIDENQPLALAILSHITDNLAYALMNLVNIFSSEKIMLNSP
                     LIKIKEPLFTQISEKLHKNLLQTNLKIDLVTSQYDWNSPLIACSAIKQGIYDGNLIKD
                     RIN"
BASE COUNT          781 a          374 c          413 g          771 t
ORIGIN      
        1 tcaatttttt cattttctta tatacaacat caacagattt tgccagaata caacctcatt
       61 ttgatatgag gaaaatagaa tgagtaagca aaagaatgat tacctccacc agcgaggtca
      121 aattaaagaa agtgcagtaa aagcattagt gactgatcca ctttttagac aacgtgtaga
      181 aagaaatcgt aaagggaaag ggagctatcg cagacaagaa aaacacaaaa aaactatcta
      241 tgggcaaaaa gaaaccccat ttaaaaatat tcggttagag tctttttaaa tggggtttta
      301 tttgtagctt attgtagttt tataagcctg ataaacacta tggtttactg aaataggcca
      361 acaacggttc tggattttga atataagaag aaagttcttt atgctctggg tgacccatat
      421 agggaatttg cccaagtaaa ggcgcattaa ttttttgtgt cagtagttca attaattcgt
      481 gataatgccg tagccctgga tttactctgt ttgcgaccca acttactaat tttacaccac
      541 gctgcaaaat ttcattcgca gtaagtagtg catggttaac acaaccttcc ttaattccca
      601 ccactaaaac aaccggcata ttgttttgca ttacccaatc agcaaaatag taatccttgt
      661 taataggagt tagccagcca taggttcctt ctacgacaac atttggatag gcattttcta
      721 gtcgttttaa atctgtgttt aatttttcca gttgaatatg atgaactgca tctaaagctg
      781 caaatacagg ggtactggaa tgaataaaag tatagctatt aatatcacga tagctaacat
      841 tagttggaca gctatctagt atggttaata cgtctgaatt atcttcagta gcataatcat
      901 attgagtagg ttctgtgggt aatgaatcat caccgccaca agcaatgggt ttatagccaa
      961 ttacaggaaa actatgtttt gtaagtgtct gtatgatggc tcgggtaacc acagttttcc
     1021 ctacgttggt gtctgttccg gtaataaata atgagggcat aaatggctcc tttcgaaaat
     1081 atggctggta gtttaaccaa aaatagatca atttattctg tctttgatca aatttccgtc
     1141 ataaattcct tgttttattg ctgaacaggc aattaatgga ctattccaat cgtattggct
     1201 ggtaactaaa tcgattttta ggtttgtttg taacaaattt ttatgcaatt tttctgaaat
     1261 ttgcgtaaat aacggctctt ttattttgat caatggcgag tttagcataa ttttttcact
     1321 agaaaatata ttaactaaat tcattaatgc ataagctaaa ttatcggtaa tatgcgataa
     1381 aatagctaat gcaagcggtt gattttcatc aattttttga caaagccagc tgattttctg
     1441 ttcctgactt tttaaattat taggcaaata acgatcaatg agtctcacaa tagctgggaa
     1501 cgtcacctga ttgactaatt tatagcgatg aatttcatct agttcaggga atatttcatc
     1561 actgagatgg ctaaatctag gcataagcat attatcaaca ttcatctttg attgtttaga
     1621 cagtaaacta ccactaagca atacacttag attcacagta tcatctaatt gtagaaaaat
     1681 agcattatct tggttaatta atgagccaac cgtagattct gcaagcaacc acagctgaaa
     1741 atgttcactt aataaaatag gacaatgaaa cagcggcgag agtttagatg taatgttaca
     1801 gaatatattt ttgtttccca attgagtaat actgtctttt tctgcattaa ttttaccgat
     1861 cacactaata gatacagcca ggagctgact agtattaaca caatatagtt ggcggaaatt
     1921 tgtaataaag ttaagaataa aatcatttag cctaggataa ttttctgctg agattgtata
     1981 atcttgctta taaatttctg caccattgag tttacaaagt gaaatttcaa ttttatgaga
     2041 tgaaacagta acacaaagta gttgccaaaa aaaattagaa acagctaatc caacggaggg
     2101 gcgacctctg gataaagtag tctgaaccgt acgttctaaa atgaagtggt tatctattag
     2161 tattttagtc agatttgtta ctgaagctgg ggctaacccc gatagtttag ctaaatctgt
     2221 tcttgaaatg agttcaaatt gctcaattaa atggtatatt ttacccaaat gaagggcttt
     2281 gttgtcagaa aacatttaaa atccttatgt tagaattttt agatgacaat tattttttg
//
LOCUS       DANLXS010000265         2316 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.273, whole genome shotgun sequence.
ACCESSION   DANLXS010000265 DANLXS010000000
VERSION     DANLXS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.273"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1083)
                     /locus_tag="PXD71_002574"
     CDS             complement(<1..1083)
                     /locus_tag="PXD71_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL6292632.1"
                     /translation="MSKKKKHFLANPLLWLTVIIPTACSGIYYSLFASDIYVSESSFV
                     VRSPNNQSSLTGVGALLQNSGFARSQDDTYSVQEYMRSRTALEQLEAEIPVKRFYSEQ
                     GDLLSKFNGLGFNDTQEAFYRYFKERLNIDVDSISGIATLRVRAFNAEEGQKINENLL
                     QQGETLINRLNERARQDTVEFAEERVKEAEKDVNNTALALSQYRTKNKIYDLPAQSSV
                     QLSLISTLKSELIRVETQLAQLKSITPDNPQVAALQMRQKSLKKEIDDQSKQLAGGNT
                     SVATQTAEYQRLVLANELAQQQLAAAMTSLQNTRGEADRQQLYLEVIGRPSKPDWALE
                     PYRIYNIISTFFIGLILFGVLSLLLAS"
     gene            complement(1155..2303)
                     /locus_tag="PXD71_002575"
     CDS             complement(1155..2303)
                     /locus_tag="PXD71_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDL6292633.1"
                     /translation="MTLSLISACSSLPTSGPSYSEILASNENTAETQLPEVNLIKLDN
                     ITVQNLYQEQQSQRFSGFDGSMGVGGYAGTVNVGDILEISIWEAPPAVLFGGTFSAEG
                     QGNGHLTQLPQQMVNKNGTVTVPFIGNIKVAGKTPEVIQNQIVGALSRKANQPQALVR
                     IANNLSSDVTVIRQGNSIRMPLSANNERILDAVAAVGGTSENIEDVTVRLTRGNKVRS
                     MAFETLIADPSQNITLRSGDVIALMNTPYSFTGLGAVGNNQQMRFSSKGITLAEAIGK
                     MGGLIDERSDPRGVFVFRHIPFTQLDYDNQAQWREKGYAEGMDVPTVYQVNLLKPQSM
                     FLLQRFPIQDKDIVYVSNAPLSEFQKFLRMIFSITTPITGTANTIRAY"
BASE COUNT          694 a          450 c          412 g          760 t
ORIGIN      
        1 acttgctagt aacaaactta atactccaaa tagaattaag ccgataaaaa aagttgatat
       61 aatattataa attctgtagg gttctaatgc ccaatctggt ttgcttggcc taccaattac
      121 ctctaaataa agttgttggc gatcagcttc acctcgtgta ttttgtaatg aagtcattgc
      181 tgctgcaagt tgttgctgag ctaattcgtt agcaagaacc aaacgttgat attcagcagt
      241 ttgcgttgct acagaagtat tacctcctgc aagttgtttg gactgatcat caatttcttt
      301 tttcaaactt ttttgacgca tttgtaatgc ggctacctga ggattatccg gcgtaattga
      361 cttcaattgt gctaattgtg tttctacccg aattaattca ctttttaagg tagaaattaa
      421 tgagagctga acgctggatt gtgccggtaa atcataaatt ttatttttag ttcgatattg
      481 gcttaatgct aacgcagtat tattgacgtc tttttcggcc tcttttacac gctcttctgc
      541 aaactcaacc gtatcttgtc ttgctctttc attaaggcgg ttaattaatg tttccccttg
      601 ctgtaataaa ttctcgttaa ttttttgtcc ttcttctgca ttaaaagcac gaacgcgtag
      661 cgttgcaata cctgaaatag aatcgacatc aatattcaga cgttctttaa aataacgata
      721 aaatgcttct tgggtatcgt taaatcctaa accgttaaat ttgcttagta aatcaccttg
      781 ctcactataa aaacgcttta ccggaatttc tgcttcaagt tgttctaatg cggtacggga
      841 acgcatatat tcctgaactg aataggtatc atcttgagaa cgggcgaaac cggagttttg
      901 caataaagca ccaacgcctg ttaagctcga ttgattattg ggcgaacgaa ccacaaagct
      961 agattctgaa acataaatat cagaggcaaa taaggaataa taaattccag aacaagcagt
     1021 tggaataatt acagttagcc acaacaaagg gttggctaaa aaatgttttt tcttctttga
     1081 cataaaatcc tcaacgaaaa gtgttacaag cggtcatttt ttaatgattt tttaccattt
     1141 tatctatgag ctaattagta cgctctaatt gtattagctg tacctgtaat tggtgtagta
     1201 atggagaaaa tcattctcaa gaatttttga aattcagaca gtggagcatt cgacacatat
     1261 acaatatctt tatcttgaat agggaaacgc tgcaataaaa acattgattg cggttttaat
     1321 aaattcacct gataaacagt tggaacgtcc attccctctg cataaccttt ttcacgccat
     1381 tgagcttggt tatcgtaatc taattgtgta aaaggaatat ggcggaatac gaacacacca
     1441 cgagggtctg aacgctcatc aattaatcct cccattttac cgatagcttc agcaagggta
     1501 atgcctttac ttgaaaaacg catttgttga ttattaccta ctgctcctaa tccggtaaag
     1561 ctataaggag tattcattaa agcaataaca tcacctgaac gtaatgtaat attttgtgag
     1621 gggtcagcaa ttaatgtttc aaacgccatt gaacgcactt tatttccacg tgttaaacga
     1681 actgttacat cttcaatatt ttcgcttgta ccacctactg ccgcaactgc atctaaaata
     1741 cgctcattat tggcacttaa tggcatacga atgctatttc cttggcggat aaccgtaaca
     1801 tcagacgaca agttattcgc aatacgaact aatgcttgag gttgattagc tttacggctc
     1861 aatgctccga caatttgatt ctgaattact tcaggtgttt ttccagcaac tttaatatta
     1921 ccgataaaag gcacagttac cgttccattt ttgttcacca tttgctgagg taattgggtt
     1981 aaatgcccat taccttgccc ttcagcacta aatgttccac caaatagcac ggcaggtggc
     2041 gcttcccaaa tggatatttc taatatatcg cctacattaa ctgttccagc ataaccaccc
     2101 acacccattg agccatcaaa tcctgaaaaa cgttgagatt gttgctcttg gtataagttt
     2161 tgtacggtga tgttatctaa cttgattaaa ttgacttcag gcaactgcgt ttcagctgta
     2221 ttttcattac ttgctaagat ttcgctatag cttgggccgg acgttggtag gctagagcag
     2281 gctgaaatta atgataatgt tataaataat aatgta
//
LOCUS       DANLXS010000266         2292 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.274, whole genome shotgun sequence.
ACCESSION   DANLXS010000266 DANLXS010000000
VERSION     DANLXS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.274"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            98..526
                     /locus_tag="PXD71_002576"
     CDS             98..526
                     /locus_tag="PXD71_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL6292634.1"
                     /translation="MTHDWLYWALASALFAALTAIFAKVGLQGVDSDFATFIRTVVII
                     IALIAFLTYSGKWQPLDSLSSKNWLFLILSGLATGLSWSAYFKALQMGNASQVAPIDK
                     FSLVLVTLFAVIFLGERPSGQDWLGIGLVAGGVLLLAFKR"
     gene            532..1254
                     /locus_tag="PXD71_002577"
     CDS             532..1254
                     /locus_tag="PXD71_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDL6292635.1"
                     /translation="MQNFANIEIAFIHRKAPLPSDFSYPEMPGGLTEKQVQKWQSRRV
                     AFFLLTELFKKHHLDLDLLADMQRSPSGRTFVDSEQIDFNISHSGEWIAVIFSYSFAK
                     LAVGIDIEHPQKIRRYHDLICHYGNAEEQNVLLANHSPILSNLAERFYLSWCLREAIL
                     KSQGVGIVKLSEVKHLPLEKQIFSAHCPKGKLHFVSELPFYLSYFYQQPQNMLLSEPL
                     LYHWQNGQFQPIKCQSLVYDVN"
     gene            1264..1683
                     /gene="psiE"
                     /locus_tag="PXD71_002578"
     CDS             1264..1683
                     /gene="psiE"
                     /locus_tag="PXD71_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDL6292636.1"
                     /translation="MPKKSLEEHHSSFGKIASETFQWIMNIALLVTGALLSYSLFAEA
                     YSLYELSFSDSEKFQIVEKIVIFFLYFEFLALIVKYFKSNYHFPLRYFLYIGITAMVR
                     LIIVDHSNALHTLLFALSILVMIIALYIVHTDRLHKS"
     gene            1692..>2292
                     /locus_tag="PXD71_002579"
     CDS             1692..>2292
                     /locus_tag="PXD71_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL6292637.1"
                     /translation="MKLTQYQHNDLQILNRETVYKGHFELQKITFRHKLFSGEMSGEV
                     VRELLIKGEAAALIAYDPTLDKVVLIEQVRIGAYDAGQKKSPWLLELVAGMVDKGNEE
                     PAEVAMREAKEEAGLEVTTVQHALSVWDSPGGQLERLHLFLGLIDSTQVKSGEVHGLL
                     EEENEDILVHVVSREQAYQWVEDGTIDNVIAVIGLQWLQL"
BASE COUNT          660 a          460 c          483 g          689 t
ORIGIN      
        1 ttgcgaagat caaatagaaa atttgataaa ctagaactaa ctcgcatttt attgctccat
       61 atgattagaa attaacctaa aatattaaaa ttttgttatg acacacgatt ggctctattg
      121 ggcattagcc tctgcacttt ttgcagcatt aaccgccatt tttgccaaag ttggcttaca
      181 aggcgtagat tctgatttcg ccacttttat tcgcacggta gttattatta ttgcacttat
      241 tgcctttctc acttacagcg gaaaatggca gccgttagat agcttgagca gcaaaaactg
      301 gctgttttta atcttatccg gcttggcaac aggattgtct tggtcggcgt atttcaaagc
      361 attgcaaatg ggtaatgctt cccaagtcgc tccaatcgat aaattcagct tggtgttagt
      421 tacccttttt gcagtaattt tcttaggcga acgcccaagc ggacaagatt ggcttggcat
      481 tggcttagtt gctggtggcg tactgttact tgccttcaag cggtgatttt tatgcaaaat
      541 tttgcaaata ttgaaattgc gtttattcat cggaaagcac ctctgccgag tgatttctcc
      601 taccctgaaa tgcccggcgg tttaactgaa aaacaggtgc aaaaatggca aagccgccga
      661 gtcgcctttt ttctgctgac ggaattgttt aaaaaacacc atctggattt agatttatta
      721 gctgatatgc aacgctcccc aagcggtcgc acttttgtcg attctgagca aattgacttt
      781 aacatcagcc attcagggga atggatagcg gtcatttttt cgtattcttt tgcaaaatta
      841 gcggtcggca ttgatattga acacccacaa aaaatcagac gttaccacga tttaatctgc
      901 cattacggaa acgctgaaga acaaaatgtt ttattagcca accactcccc cattctaagc
      961 aacctcgccg aacgctttta tttgagctgg tgcttgcgag aggcgatatt gaaatcgcaa
     1021 ggggtgggga ttgtgaaact gtccgaagta aaacatttac cgttagaaaa acagatcttt
     1081 tccgcccatt gtccgaaagg caagctgcat tttgtgagcg aactgccgtt ctatttaagc
     1141 tacttctatc aacaacctca aaatatgcta ttatctgaac ctttgctata ccattggcaa
     1201 aacgggcagt ttcagcctat aaagtgccaa tcacttgttt atgatgttaa ttaaggataa
     1261 cgaatgccta aaaaatcttt agaagaacac cattccagtt ttggcaaaat tgcttccgaa
     1321 acctttcagt ggattatgaa tattgccttg ctagtaacag gagcattgct ttcttactcg
     1381 ctgtttgctg aagcctattc actctatgaa ctttccttta gcgacagtga gaaattccaa
     1441 attgtagaaa aaatagtaat tttctttctc tattttgagt ttcttgcgtt gattgtgaaa
     1501 tatttcaaat cgaattatca ctttccgtta cgctatttcc tctatatcgg gattactgcc
     1561 atggtgcgtt tgattattgt cgatcattct aatgcgttgc atacactatt attcgcactc
     1621 tctattttgg tgatgattat agcattatat attgtacaca ccgatcgttt acataaaagc
     1681 taggaactaa tatgaagcta acccaatatc agcacaatga tttacagatt ctaaaccgag
     1741 aaaccgtcta taaagggcat tttgagctac aaaaaatcac tttccgccat aagctgtttt
     1801 ccggtgaaat gagcggtgaa gtggtgcgtg aactcttaat taaaggcgaa gcggcggctt
     1861 taattgccta tgatccaacc ttagataagg tagtattaat tgagcaagtg cggattggag
     1921 cttacgatgc aggacagaaa aaatcccctt ggttgctgga attggttgcc ggtatggtag
     1981 ataaaggcaa cgaagagccg gcagaagtgg caatgcgtga agccaaagaa gaagccggct
     2041 tggaagtcac aacagtgcaa cacgccttaa gcgtatggga cagtcccggt ggacaactgg
     2101 aacgcttgca cctgtttctc ggcttgattg acagcaccca agtgaaatcc ggcgaggtac
     2161 acggcttact ggaagaagaa aatgaagaca ttttggttca cgttgtaagc cgtgaacagg
     2221 catatcaatg ggtagaagac ggcacaattg acaatgtgat tgcggtaatt gggctacaat
     2281 ggttgcaatt aa
//
LOCUS       DANLXS010000267         2287 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.275, whole genome shotgun sequence.
ACCESSION   DANLXS010000267 DANLXS010000000
VERSION     DANLXS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.275"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..214)
                     /gene="raiA"
                     /locus_tag="PXD71_002580"
     CDS             complement(<1..214)
                     /gene="raiA"
                     /locus_tag="PXD71_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL6292638.1"
                     /translation="MSLNISSKQMDITPAIRTHIEERLAKLEKYHTQLINPHFMLIKQ
                     PNSFQVEATIGTPLGDLVAKAEHEDLY"
     gene            394..1335
                     /locus_tag="PXD71_002581"
     CDS             394..1335
                     /locus_tag="PXD71_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HDL6292639.1"
                     /translation="MSEKKQLPPFQTQFLHPKYWGLWLGLGVFKLILCLPYPILVKIG
                     LGLGKLFTKLGFGKRRMRIARRNLELCFPHYSAEKVEELVEENMKSVGMAIIETGMAW
                     FWSDKRILKWSKIEGLEHLTQRDQERGILLVGVHFLTLELGARIIGLHHQGVGVYRPN
                     DNPLLDWIQFRGRVRSNKAMLDRKDVRGMIRALKNGEMIWYAPDHDYGRRNSVFVPFF
                     AVEETCTTAGSYMLLRSAPKSLVIPFSPIRNLDYSGYTVKVSPAVDFSQCQNEVETAI
                     LMNRVVEREIMAALTQYMWLHRRFKTRPNEGDQSLYD"
     gene            1431..>2287
                     /gene="dapA"
                     /locus_tag="PXD71_002582"
     CDS             1431..>2287
                     /gene="dapA"
                     /locus_tag="PXD71_002582"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL6292640.1"
                     /translation="MATPLFHGSIVALVTPMTHGEVDYQDLKKLVEYHIQAGSHAIVS
                     VGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGTGSNATSEAITMTKLLANSGVAG
                     CLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPGRTGSDLKPETIGRLAKIENI
                     VAVKEATGDLSRLPLIKQLAGEDFIFLSGDDATGFESMKLGGQGVISVTNNVAAADMA
                     KMCELALAGQFTEAEEINNRLMALHHDLFVESNPIPVKWAAYKLGLISEPNLRLPLTT
                     LSESAQPVV"
BASE COUNT          695 a          357 c          498 g          737 t
ORIGIN      
        1 tatataaatc ttcatgttca gctttagcga ctaaatcacc taaaggggtg ccaattgttg
       61 cttcaacttg gaatgagtta ggttgtttta tcagcataaa atgtgggttt atcaattggg
      121 tgtggtattt ttctaactta gctaaacgct cctcaatatg agtacggatt gcaggggtaa
      181 tgtccatttg tttgcttgaa atatttaatg acataacatt ttcctcttgt ttagttaagg
      241 cttttgccgt tgatattcta ataaccaaaa tatgctcatc actccaaaaa ttcaagtgaa
      301 aaatgaaata agtttccaaa aatttgatct acttaacact tttagcagtt tttagctata
      361 atgtaatcat aatctagtat gtgagaataa ttaatgagtg agaaaaaaca gttacctcca
      421 ttccagacac aatttttaca ccctaaatat tggggattgt ggctcggttt aggtgttttt
      481 aagttaattt tatgtttgcc ttatcctatt ttagtaaaaa ttggtctggg actaggtaaa
      541 ttgtttacta aattgggttt tggtaaacgt cgtatgcgta ttgctcgccg taatttagag
      601 ctctgttttc cccattactc tgctgagaaa gtagaggaat tagtcgaaga aaatatgaaa
      661 tcggttggaa tggcgattat tgaaactggt atggcttggt tttggtcgga taaacgtatt
      721 cttaaatggt ctaaaatcga agggctagag catttaacac agcgtgatca ggaaaggggt
      781 attttacttg taggggtgca ttttctgact ttagaactcg gtgcaagaat tattgggcta
      841 catcatcaag gagtaggggt ttatcgaccg aatgataatc cattattaga ttggatacag
      901 tttcgaggac gtgttcgctc aaataaagcg atgttagatc gtaaagatgt gcgtggaatg
      961 attcgagctt taaaaaatgg cgagatgata tggtacgcgc ctgatcacga ttatggaaga
     1021 cgaaatagcg tttttgtacc attttttgca gtggaggaaa cttgtactac tgcgggtagt
     1081 tatatgttac ttcgttctgc cccaaagagt ttagtgatcc cgtttagccc gattcgaaat
     1141 ttggactatt caggctacac agtaaaagtc agtcctgccg ttgattttag tcaatgtcaa
     1201 aatgaagttg aaacggctat tttaatgaat cgggtggtag aacgtgaaat tatggctgcg
     1261 ctaactcaat atatgtggct acatcgccgt tttaaaactc gcccaaatga aggtgatcaa
     1321 agcctatatg attaatttta aataatttca aaaaagtgct tgcatattta agctgaattt
     1381 tgcaatctag caatgttatc aagcataata aaaaaataca ggagatgatt atggcgaccc
     1441 ctttatttca cggtagtatc gtagcattag ttacaccaat gactcatggt gaggtagatt
     1501 atcaggattt aaaaaaatta gttgagtatc atattcaagc aggttctcac gctattgtat
     1561 ctgttggtac aacaggtgaa tctaccacat taagcattga tgaaaatgtt aaggtgatta
     1621 agaaaacggt tgaatttgct gatggacgta ttccaattat tgcaggaact ggttctaatg
     1681 cgaccagtga agctatcact atgactaaat tattagcaaa cagtggtgta gctggttgtt
     1741 taagtgtagt gccttattac aacaagccaa cgcaagaggg gatgtactta cactacaaag
     1801 cgattgcaga aagtacagat ttaccacaaa ttctgtataa tgtgccgggg cgaacaggga
     1861 gtgatttaaa acctgaaaca atcggtcgct tagcgaaaat tgagaatatt gttgctgtga
     1921 aagaagcaac gggtgattta agccgcttac ctttaattaa gcaattagcc ggtgaagatt
     1981 ttattttctt aagtggtgat gatgctaccg gttttgagtc tatgaaatta ggtggtcaag
     2041 gggtgatctc ggtcaccaat aatgttgctg ctgctgatat ggcaaaaatg tgtgagttag
     2101 ctcttgccgg tcaatttact gaagcagaag aaattaataa ccgcttaatg gcgttacacc
     2161 atgacttatt tgtcgaatct aacccgattc cggtgaaatg ggctgcttat aaattaggtt
     2221 taattagtga gcctaattta cgtttaccgc ttaccacact gtctgaatca gcacagcctg
     2281 tagtgtt
//
LOCUS       DANLXS010000268         2241 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.276, whole genome shotgun sequence.
ACCESSION   DANLXS010000268 DANLXS010000000
VERSION     DANLXS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.276"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(345..1586)
                     /locus_tag="PXD71_002583"
     CDS             complement(345..1586)
                     /locus_tag="PXD71_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDL6292641.1"
                     /translation="MMNKRIYVFADWETFSQPELVGVLSSDVVRGKENFRFSYDNEWL
                     SSPYVQQIDPNLYLYPGEQYSTDTQNFHIFLDSCPDRWGRLLMKRREAALASQENRKP
                     KLLIESDYLLGVHDMNRMGALRFKTELNGPFLDNNTRLAAPPLSSLRELEFAAIQLES
                     SDNVDDPDYLKWLHMLISPGSSLGGARPKASVIDELNQLWIAKFPSRYDDYDIALWEY
                     LAYRLAIQAKIEMATCRIERFNQEHHTFLTQRFDRHCGKRRHFTSALTQLGYYDGDYE
                     ASYLEIAQFLTESGSNTRIDLAQLWRRIVFNIAISNTDDHLRNHGFIYAQEGWILSPA
                     YDLNPVPLSQGLHLNITDSDNRLDYQLAFDVADFFQLSKQQAVQIYDEVLKEINNWQN
                     VAKEIGIPRQEQEQMKFAFNV"
     gene            complement(1586..1897)
                     /locus_tag="PXD71_002584"
     CDS             complement(1586..1897)
                     /locus_tag="PXD71_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6292642.1"
                     /translation="MVIIMLSKTKSPTLFPKYQNALLQLGENIRLASKRRMFTQTLLA
                     QRTGLSRVTVRKIMNGDPTVAIGYYLMVLGVLGLEQDFTDVGKDDELGRKLQDIELLR
                     K"
BASE COUNT          682 a          425 c          394 g          740 t
ORIGIN      
        1 taaatgttag tttttctgat gtctgccatc tgctaggcat tatatatgcc tagattagtt
       61 tcctacattt gcgtttttaa ggctacgctt ccttgcggat atggttgaga aaaaagtcaa
      121 gtgttactaa tggttttata agatgttggt ttgttctggt gtctgccacc gctaggtctt
      181 tataccctag attagtatca ggtctttctg gtgcctgcca ccgctaggcc tttacatcct
      241 agattaatat cagattgaca atgagccaac aactcattgc gttattaagg taacgctact
      301 tttttagaac atagactacg atgattggtt ccacaagtca attactatac attaaatgca
      361 aatttcatct gctcttgctc ttggcgtgga ataccaattt cttttgcgac attttgccaa
      421 ttattaattt ctttcagtac ttcatcataa atctgtacgg cttgttgctt agataattga
      481 aaaaagtcag caacatcaaa ggcgagctga taatccaatc ggttatcgct gtctgttatg
      541 tttaaatgta atccttgaga tagtggtact gggtttaaat cgtatgcggg agataatatc
      601 cacccctctt gagcgtagat aaatccgtgg ttacgcaaat gatcatcagt attcgaaata
      661 gcaatattga atacaattct tctccatagt tgagccaagt caattcgagt atttgaacca
      721 ctttctgtta aaaattgagc aatctcaagg tagcttgcct catagtctcc gtcataataa
      781 cccagctggg ttaatgctga cgtaaaatgg cgacgttttc cacaatgtcg atcaaatctt
      841 tgtgtcaaaa aagtatggtg ttcttggtta aagcgttcaa ttctacaagt agccatttca
      901 attttggctt gaatagccag tcgataagct aaatattccc aaagagcaat atcataatca
      961 tcgtaccgac ttggaaattt ggcaatccat agttgattta gttcatctat taccgaagct
     1021 tttggtctag ctccgcctag tgacgaccct ggtgatatca acatatgaag ccattttaaa
     1081 taatccggat cgtccacatt atcactgctt tcaagttgta ttgctgcaaa ttctaattct
     1141 cttaaggaag acagtggtgg tgctgcaaga cgagtattat tgtccaaaaa tggaccattc
     1201 aattcagttt taaaacgtaa agcccccatt ctattcatat cgtgtacgcc taaaaggtaa
     1261 tcactttcta ttagtaactt aggtttacga ttctcttgac tagctagagc tgcctcccga
     1321 cgtttcatta acaatctacc ccatctatca gggcaagaat ctaaaaagat atgaaaattt
     1381 tgtgtatctg tactgtattg ctctccaggg taaaggtata agttaggatc aatctgctgt
     1441 acatagggtg aacttaacca ctcgttatcg tagctaaaac ggaaattttc tttgcctctc
     1501 actacatcag aagaaagcac cccaactaat tcaggctgtg aaaaagtttc ccaatctgca
     1561 aacacataaa ttcgtttatt catcattatt tccttaataa ttcaatatct tgcaatttac
     1621 gtcctaactc atcatccttt ccaacatcag tgaaatcttg ttctagaccg agaacaccta
     1681 aaaccattag gtagtatcca atcgcaacag ttggatcacc attcataatt tttctcactg
     1741 ttacacggct aagcccagtt ctttgagcca ataatgtttg agtaaacatt ctcctttttg
     1801 aggctagcct aatattttct ccaagttgaa gcaaggcatt ttggtactta ggaaacagag
     1861 ttggtgattt tgttttactg agcattatta ttaccatttt tatgtttttt ggaaattata
     1921 atgtccattt atagcaattg caagagcagt tatgtaaaca gtaaaatagg tagagagggt
     1981 ttttcggaaa aaaatatctg gtgaagtttt gactaaattt gaattaaaag aaaaaataga
     2041 ttttttcaaa taaaatcaat ctaaaaaatg attttcaggc tttacaaact tgaaagaatg
     2101 tgtaagatct agaacgtctg aaattcggac ggaaaatccc aatggatgta ttggctggat
     2161 acatcgtagt aactgtgtat ttgcgaatat gatttgaaca atcctacaca aacgattcaa
     2221 aatccgccgc ccataaagcg t
//
LOCUS       DANLXS010000269         2229 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.277, whole genome shotgun sequence.
ACCESSION   DANLXS010000269 DANLXS010000000
VERSION     DANLXS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.277"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..234
                     /locus_tag="PXD71_002585"
     CDS             <1..234
                     /locus_tag="PXD71_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292643.1"
                     /translation="GSSITADKLNVNNLAAISANLGAITAGSININNRFKVSNAGVVE
                     MRANSGNVGMVMNNNSIIVYDEQGRVRVKMGKL"
     gene            234..677
                     /locus_tag="PXD71_002586"
     CDS             234..677
                     /locus_tag="PXD71_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292644.1"
                     /translation="MLIGILCIIALAVLGLLFYFHKKKRKKVNKGVNMAEYGIQTFDT
                     ANVSTFSTNERLFKYIGTKNIKYGKFSFTVTEKYEGQLVFIISMLHSVHRKQATGYKT
                     NIPNLIHLENVSINDKTISGEVVCIFPFSLPADDSDYIQIVYGAY"
     gene            678..1277
                     /locus_tag="PXD71_002587"
     CDS             678..1277
                     /locus_tag="PXD71_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292645.1"
                     /translation="MSYGLSTFNINENFRSLSLQRKGRANFSNKIATIQAASDEIIVI
                     KYSDGQVCLLYRENGIATLATENATFAEYLIFSTNVPKSGGYGIEVYNSAGNVVFSSN
                     HKFLRPIEYVDLNPSKNVFRFTGQANRQYGIILSNFGFDFVINYDNGYSTSRSAKVSG
                     NFIEFGFCKYHYSGLYKTGMSYNSKAMYFNALVVDITGY"
     gene            1392..1685
                     /locus_tag="PXD71_002588"
     CDS             1392..1685
                     /locus_tag="PXD71_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292646.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYKHLKETGQIVV"
     gene            1846..2085
                     /locus_tag="PXD71_002589"
                     /pseudo
     CDS             1846..2085
                     /locus_tag="PXD71_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          778 a          346 c          427 g          678 t
ORIGIN      
        1 gggagttcga tcactgcgga taagctaaat gtaaacaatt tagcggcaat atcagcgaat
       61 ctcggagcta ttacagctgg ttctattaat atcaataata gattcaaggt aagtaatgct
      121 ggagtagtcg aaatgcgagc caatagtgga aatgttggta tggtaatgaa taacaatagt
      181 attattgttt atgatgaaca aggtagggtt agagtaaaaa tggggaaatt atgattctaa
      241 taggtatttt atgtattatt gcattagcag tcttagggct gcttttttat ttccataaaa
      301 aaaaacgaaa aaaagtaaat aaaggggtaa atatggctga atatggtatt caaacttttg
      361 ataccgctaa tgtctctaca tttagtacca atgagcgatt atttaaatac attggaacta
      421 aaaatattaa gtatggtaaa ttttcattca cggtaacaga aaagtatgaa ggacaacttg
      481 tatttatcat tagtatgtta cattcagttc accgaaaaca agcaacaggt tataaaacga
      541 atattcccaa tcttattcat ttagaaaatg tttcgatcaa cgataaaacg atttcaggag
      601 aagttgtttg tatttttcct tttagtctac cggctgatga ttcagattat attcagattg
      661 tatatggagc atattaaatg agttacggac tatctacttt taacatcaat gaaaatttca
      721 gaagtttatc cttacaaaga aaaggacgag ctaacttttc taataaaatt gcaactattc
      781 aggcagcgtc agatgaaatt attgtcatta aatattctga tgggcaagta tgtttgctat
      841 atcgagaaaa tggtattgcc acattagcta cagaaaacgc aacatttgct gaatatctca
      901 tttttagtac aaatgtaccg aaaagcggag gttacggcat agaggtttat aactcagcag
      961 gaaatgttgt attttcatca aaccacaaat ttttaagacc tattgaatat gtcgatctaa
     1021 atccatcaaa aaatgttttt agatttacag ggcaagcaaa taggcaatat ggcattatct
     1081 tatccaattt tggctttgat tttgtcatta actacgataa cggatattca acatctagat
     1141 ctgccaaagt aagtggtaat tttattgaat ttgggttctg taaataccat tattctggtt
     1201 tgtataagac gggaatgagt tataattcaa aagcaatgta ttttaatgct ctggttgtag
     1261 atataacagg ctattaaaca ttaaacgcct tcatttaata gaggcgaact aaaattctga
     1321 ttacacattc atcgccgctt gtagcaatac aagcggtctt tttttatcaa caatccacaa
     1381 aaggaaaaat tatgacaact tttaacaaaa tcttaaaccc aatgtactca actatcgcaa
     1441 gttactcaac tcaagatgac ggctcactca atgctaagta tgttgttggc acaggtgatg
     1501 ataccgatgg cgaggtaaca aactttgtaa tcatcacaag tgagtacaaa tatattgatg
     1561 ctcaatcagc caaagcgatt acagatgcac cgctaacaaa agaggatatt ggcaaaacgc
     1621 caacgcagat tatgttaggt cgtatctata agcacttaaa agagacgggg cagattgtag
     1681 tgtaggggtc taaatgcaaa agcataaatt taagtttttc agaggcgatg acgtaagtta
     1741 tcgccttttt tttaggcaaa caaatggcga gcctgtaaat ttagagggta ttaggctgga
     1801 tttacacgcc atagtagagg gcagcacaga gcctcaaatt gagctaaccg tcgattagtg
     1861 gttgtggatg agcaatcatt aaaactgcaa gagttaaagc agactattaa tgagcaaacc
     1921 gagcaaatta aaacactcgg aacagtggta aataaactta ttgatgcatt acaacagtca
     1981 caaagtttaa atgtaagatc agaatctgct gtagcagcaa ccgaaaatca agatcattta
     2041 actgatggtg tgcagtatca aggcccagga cgtgctagag cttaactctg ttaacatctg
     2101 cactcaaaac ggctatagct ctagccgttt tttgtaattt ttcgcacgtt ttaacaggca
     2161 aatttggcaa aattagccat tgtgatttta ttttctataa ttaagccccg agaggggctt
     2221 taaagtcag
//
LOCUS       DANLXS010000270         2161 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.278, whole genome shotgun sequence.
ACCESSION   DANLXS010000270 DANLXS010000000
VERSION     DANLXS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.278"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(79..1212)
                     /locus_tag="PXD71_002590"
     CDS             complement(79..1212)
                     /locus_tag="PXD71_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis PLP-dependent protein"
                     /protein_id="HDL6292647.1"
                     /translation="MTKFNNIETTLVQIGNRTDPRTGAVAMPIILSTAYGRDGLGEST
                     GWDYTRTKNPTRAVLEQGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVY
                     GGSYRLFDFSHKNHGTLKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVAA
                     IAKIAKKHNLMLIVDNTFLTPALFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDS
                     DATKVNGQNLTERLFYFQNCAGAVLSPFDSYLAVRGMKTLALRMERHQSNATELAKFL
                     AEQPEIDSVLYSGKSGMLSFRLQKEEWVAKFLKAIKLITFAESLGGAESFITYPATQT
                     HMDIPEAERITRGITNNLLRFSVGLEHVEDIKADLLQAFKQLK"
     gene            complement(1229..2041)
                     /gene="dapB"
                     /locus_tag="PXD71_002591"
     CDS             complement(1229..2041)
                     /gene="dapB"
                     /locus_tag="PXD71_002591"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL6292648.1"
                     /translation="MTLKIGIVGAGGRMGRQLIQAVHNAQGVELGSAFERKGSSLVGS
                     DAGEVAGVGTLGVVISDDVAQNVAGFDLLIDFTRPEGTLEHLDICVAHHKQMVIGTTG
                     FDDAGKAAIKAASEKIGIVFASNYSVGVNLVFKLLEKAAKVMGDYCDIEVIEAHHRHK
                     VDAPSGTALSMGEHIAKTLGRDLKTHGVFERNGITGERKRDEIGFATIRAGDVVGEHS
                     VWFADEGERVEIAHKASSRMTFANGAVRAAKWLNGKQNGLFDMTDVLDLNNL"
BASE COUNT          626 a          508 c          385 g          642 t
ORIGIN      
        1 tattaaaaaa gaaaacggtc agattggtaa aatctatacc taatatgacc gcttgtgaat
       61 acaaaaaaat tacttcaatt acttcaactg tttaaaggct tgcagtaaat cagctttaat
      121 atcttcaacg tgttctaaac caacggaaaa acgcagtaaa ttattcgtaa tgcctcgtgt
      181 aatacgttct gcttccggaa tatccatatg ggtttgtgtt gcaggataag taataaaact
      241 ttctgcaccg cctaaacttt ccgcaaaggt aatcaattta attgccttga gaaatttcgc
      301 tacccactct tctttttgta aacggaaaga taacatacca cttttgcctg aatataacac
      361 gctgtcaatt tcaggttgct ctgctaagaa tttagccagt tcggtggcat tagactgatg
      421 gcgttccatt cgtaatgcca aggttttcat accacgcacg gcaaggtaag aatcaaatgg
      481 cgacagcact gcaccggcac agttttggaa atagaataaa cgctcggtca aattctgccc
      541 gtttactttg gtagcatcag aatctttcgc aacaattaag ccggctaaca catcattatg
      601 ccccgaaaga tacttggtcg cactgtgaat cacaatatca gctccgtgct caatcggacg
      661 gaataacgct ggggttaaga acgtattatc aacgatcaac attaaattgt gttttttcgc
      721 aattttagca atggcagcaa cgtcgcactc ctccatcaat ggatttgacg gcgtttccac
      781 gaaaatcgcc ttagtattcg gtgtaatagc tgcttcaatc gctgctaaat ccgctgtatt
      841 aacataaacc ggctttaatg tgccgtgatt tttgtggcta aaatcaaaca agcggtaaga
      901 accaccataa acatcgctgg aaataatcca ttcatcaggt gctttaaata atgacattac
      961 tagctgaatc gctgccattc cagatgccat cgcaaaaccg gcatcaccgc cttctaaatc
     1021 cgcaatcccc tgctctaata cagctcgagt tgggtttttg gtacgggtgt aatcccagcc
     1081 ggtagattcg cctaagccat cacgcccgta agcagttgat aagataatcg gcattgccac
     1141 cgcccctgta cgaggatcag ttctgttacc gatttgcact aatgtggttt caatattatt
     1201 gaattttgtc attgttatta tccttcaatt acagattatt tagatccaat acgtctgtca
     1261 tatcaaacaa gccattttgt ttgccattta accattttgc agcacgcact gcgccattgg
     1321 caaacgtcat tcgactagag gctttatgtg cgatttcgac acgttcgcct tcatcagcga
     1381 accatacact atgctcccct acaacatcac cggcacgaat agtagcaaag ccgatttcat
     1441 cacgtttgcg ttcgcctgta ataccgttac gttcaaatac gccatgagtt ttcaagtcac
     1501 gcccaagagt tttggcaata tgttctccca ttgataaagc cgtacctgat ggtgcatcaa
     1561 ctttatggcg atggtgtgcc tcaatcactt caatatcaca ataatcgccc attacttttg
     1621 ctgctttttc gagcagttta aacaccagat tcaccccaac gctgtagttt gaggcaaaaa
     1681 cgataccgat tttttcactc gctgctttga ttgcagcttt acctgcatca tcaaaaccgg
     1741 ttgtgccaat caccatttgt ttatgatgag ccacacaaat atccaaatgt tctagcgtgc
     1801 cttcagggcg agtaaaatca attagtaaat caaaacctgc tacattttgg gcgacatcat
     1861 cagaaatcac cacacctaat gtaccaaccc cggctacttc accagcatca ctacctacta
     1921 atgatgaacc tttacgctca aaagcagacc ctaattctac gccttgtgca ttatggacgg
     1981 cttgaattaa ttgacgcccc attcgcccac cggcaccgac gattccaatt tttaatgtca
     2041 tatttgttct tcctttttat atttgttttt tacaagcggt tacattttta attaattttg
     2101 caaatagctt tctgatggca atttaaaagg tgggtgagaa gtaaaatcaa caatttgaat
     2161 a
//
LOCUS       DANLXS010000271         2118 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.279, whole genome shotgun sequence.
ACCESSION   DANLXS010000271 DANLXS010000000
VERSION     DANLXS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.279"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1902
                     /locus_tag="PXD71_002592"
     CDS             <1..1902
                     /locus_tag="PXD71_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDL6292649.1"
                     /translation="IFLKSGKNGDIPHIQCLVRKKDIKLTPEEAVRQLYLMNLHYEYD
                     YPFERMELEYSVVFGREKKRADIVIFDRDNPNAVYIMVELKKPKLKDGKEQLKSYCNA
                     TGAPIGVWTNGESISYYNRRDPNYFKDIPDIPNAQQKLSDLLQERWTIQDLIDNDKIA
                     KQRKSLKDLVIEMEDEVLANAGVDVFEELFQLIFTKLYDEMESTRNKSRYLEFRNYGD
                     TESELKAKIQSLFDRAKNKWEGVFAESAKINLSPSHLAICVSSLEEVKLFNSNLDVVD
                     EAFEYLINKSSKGEKGQYFTPRYVIDMCVKMLDPKPEETVIDTAAGSCGFPVHSIFHV
                     WEKQLKARGLERSHLFTAEEKLPEQTDYVKEKVFAIDFDEKAVRVARTLNLIAGDGQT
                     NVLHLNTLDYERWEDFTKEEEWNDVYGEGWKKLRKLRKTKNENRDFQFDVLMANPPFA
                     GDIKETRILARYELGKNSKGKQQSKVGRDILFIERNLDFLKDGGRMAIVLPQGRFNNS
                     SDKYIRDFIAERCRILAVVGLHGNVFKPHTGTKTSVLFVQKWHDELCPKVEDYPIFFA
                     TMQKSSKDNSGDKIYVKNADGTLKLDNHQHLIVEHDLFNHDNSTQDGIAEAFIEFAKK
                     EKLSFVGKD"
BASE COUNT          731 a          338 c          426 g          623 t
ORIGIN      
        1 attttcctca aatcagggaa aaatggtgat attcctcata ttcaatgtct tgttcgtaaa
       61 aaggatatta agctcacacc tgaagaagca gtacgccaac tctatttaat gaatttgcat
      121 tatgaatatg attatccatt tgaacgaatg gaattagaat attctgttgt attcgggcgt
      181 gagaagaaac gagcggatat tgtcatcttt gatagagata atcctaacgc tgtctatatt
      241 atggtggagc tgaaaaagcc gaaattaaaa gatggtaaag aacaattaaa atcttattgt
      301 aatgcgactg gagcacctat tggtgtatgg acaaatggcg aaagtatttc ttactataat
      361 cgccgtgatc ctaattattt taaagatatt cccgatattc cgaatgctca acaaaaactt
      421 tctgatttat tacaagaacg ttggactatt caagatctga tagataatga caaaattgcc
      481 aaacaacgta aatcattgaa agatcttgtt attgaaatgg aagatgaggt actggcgaat
      541 gctggagttg atgtttttga agagttattt caacttattt ttactaaact ctatgatgaa
      601 atggaaagta cacgcaataa aagtcgttat ttagagtttc gtaactatgg tgataccgaa
      661 agtgaattaa aggcaaaaat tcaatctcta tttgatcgtg caaaaaataa atgggaaggc
      721 gtatttgctg aaagtgccaa aatcaacctt tccccttcgc atttggcgat ttgtgtatct
      781 tcattggaag aagtaaagct gtttaattct aaccttgatg tagtggatga agcatttgag
      841 tacttaatta ataaatccag taaaggagag aaaggacaat actttacgcc tcgttacgtc
      901 attgatatgt gcgtaaaaat gctcgatcca aaacctgaag aaacagtcat tgataccgca
      961 gcaggaagct gtggttttcc agttcattct atttttcacg tttgggaaaa acaacttaaa
     1021 gcgagaggat tagaacgctc acacttattt actgctgaag aaaaattgcc tgaacaaaca
     1081 gattatgtga aagaaaaagt ctttgcgatt gatttcgatg aaaaggcagt acgtgttgct
     1141 cgtaccctaa acttaatcgc aggagatggg caaaccaacg tactacattt aaatacctta
     1201 gattatgaac gttgggaaga ttttactaag gaagaagaat ggaatgatgt atatggagaa
     1261 ggctggaaaa aattaagaaa attacgtaaa actaaaaatg aaaatcgaga tttccaattt
     1321 gatgtgctaa tggcaaatcc gccttttgcc ggtgatatta aagaaactcg tatattagct
     1381 cgctatgaat taggcaaaaa tagcaaagga aaacagcaat ccaaagtcgg acgagatatt
     1441 ttatttattg aacgcaattt agatttctta aaagacggtg gacgaatggc gattgtgcta
     1501 ccacaagggc gttttaacaa ttccagcgat aaatatattc gtgattttat tgcagagcgt
     1561 tgccgcattt tagctgttgt gggcttacac ggaaatgtgt ttaaaccaca taccggcaca
     1621 aaaacttccg tactatttgt gcaaaaatgg cacgatgaac tttgcccgaa agtagaggat
     1681 tacccgattt tctttgccac aatgcagaaa tccagcaaag ataattcagg cgataaaatc
     1741 tatgttaaaa acgctgatgg cacactcaaa ttagacaacc atcaacacct aattgtggag
     1801 cacgatctat ttaatcacga taatagtacc caagacggca ttgcagaagc ctttattgaa
     1861 tttgctaaaa aagaaaagct ctcttttgtg ggaaaggatt aagcccattt gaccctgcta
     1921 aatatcaggc attgttagat gggctagagg tgagcgaggt ttatttgagt aaggttcgtt
     1981 ctgcaagtta ttcggaacga atggatagta ctttcttttt gaaaaaatac ttaaaccatc
     2041 cgagttttaa ttttccatta tcgctgaatg aggtttctct gattaaaagt ggtacaacac
     2101 caacagatcg agatgata
//
LOCUS       DANLXS010000272         2098 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.280, whole genome shotgun sequence.
ACCESSION   DANLXS010000272 DANLXS010000000
VERSION     DANLXS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.280"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            51..698
                     /locus_tag="PXD71_002593"
     CDS             51..698
                     /locus_tag="PXD71_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6292650.1"
                     /translation="MISIRNVSKQYRTNNGWKTILKDINFELKKGEKVGILGRNGAGK
                     STLIRLMSGVEPPTTGTIERQMSISWPLAFSGAFQGSLTGMDNLRFVSRIYDADFDYV
                     KSFTEEFSELGEYLYEPVKSYSSGMKARLAFALSLSIEFDCYLIDEVIAVGDARFAAK
                     CKHELFEKRKDRSLILVSHSPASIKEYCDSAMVLEEGIMHTFDSIDNAYNFYNKT"
     gene            complement(776..1405)
                     /locus_tag="PXD71_002594"
     CDS             complement(776..1405)
                     /locus_tag="PXD71_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDL6292651.1"
                     /translation="MDFSKILSQVLDTAKDAATNGLIKGNSKNDQIAKIGGGAVAIGL
                     ISMLFGRNGGSSLAKLGSLAAIGSLAYQAYQKYQAQNSSVSELSQNNFENVDENVASK
                     VILQAMIAAAASDGAITEEEKAAIVSQVGDEAEVIEWIRQEMDTPATVEEIARQVGND
                     QALATQVYLASRAVCRDLERKEIVFLANLADALGLAEALVEQLEKDAGF"
     gene            complement(1477..1950)
                     /locus_tag="PXD71_002595"
     CDS             complement(1477..1950)
                     /locus_tag="PXD71_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDL6292652.1"
                     /translation="MLEKITACPCQTGKRYAECCQPFHLKQTFASTAEQLMRSRYSAY
                     TLVNIPYIVETTVPSQQKLLDQTAMKEWAENTKWVGLSVLNHLPSLSRIHSQVEFKAF
                     FDTDEGIQGHHETSLFVQIDERWFFVDPTVPLPHQKQPCVCGSGKKFKHCCGNYL"
BASE COUNT          661 a          388 c          386 g          663 t
ORIGIN      
        1 tttataggac tgctttgtgt gagaagtttt agtaaaggga tagaaccaag atgattagta
       61 ttagaaatgt aagtaagcaa tatcgaacta ataacggttg gaaaaccata ttaaaagata
      121 taaattttga gctgaaaaaa ggcgaaaaag tggggatttt agggcgcaat ggtgcaggaa
      181 aatcaacctt aatccgttta atgagcggag tagaacctcc aacaacaggt acgattgaac
      241 gtcaaatgtc gatttcttgg ccactggcat ttagtggtgc attccagggt agtttgaccg
      301 gaatggataa tcttcgtttc gtcagccgaa tttatgatgc tgattttgac tatgtaaaat
      361 ctttcaccga agaattttca gaactcggtg aatatttata tgaaccggta aaaagctatt
      421 cttctggtat gaaggcccgt ttagcctttg cattatcgct ttctattgaa tttgattgtt
      481 acttaattga tgaagtgatt gcagtaggcg atgctcgatt cgctgccaaa tgtaagcacg
      541 agctttttga aaaacggaaa gaccgttcat tgattttagt ttctcatagc ccagcctcaa
      601 ttaaagaata ttgcgattct gcaatggttt tagaagaggg tatcatgcat acttttgatt
      661 ctatagataa cgcttataat ttctacaata aaacataaaa gaacaagcgg ttatatttca
      721 caagtttttc gcaaaaaact ctcaaaatat aaccgcttgt tactaaaact gaaaattaga
      781 aacctgcgtc tttttctaat tgttcaacta atgcctcagc taaacctaaa gcatcagcta
      841 aattagctaa gaaaacgatt tctttgcgtt ctaaatctct gcatactgca cgggaagcta
      901 agtaaacttg tgtagccaat gcttggtcat tacctacttg gcgggcaatc tcttcaactg
      961 tagccggagt gtccatttct tgacgaatcc actcaatgac ttcagcctca tctccaactt
     1021 gggaaacaat agctgctttt tcctcttcag taatagcacc atctgaagca gcagcggcaa
     1081 tcattgcttg taaaatcact ttacttgcca cattttcatc tacattttca aaattatttt
     1141 ggctaagttc tgaaaccgat gaattttgcg cttgatactt ttggtatgct tgataagcaa
     1201 ggctaccgat agcggctaaa gagcctaatt ttgctaaact tgaaccgcca ttacgcccaa
     1261 ataacattga aattaaacca atagcgacag cgccgccacc aattttagcg atttgatcgt
     1321 ttttagaatt acctttaatt aatccgtttg tagcggcatc ttttgctgta tctaatactt
     1381 gacttaaaat cttactgaaa tccatttttg ttcctcttgg taagtaaatg aaatttactc
     1441 attagtcctt agattcaaac aagagttcaa ttcaaattaa agataatttc cacaacagtg
     1501 cttaaacttc tttccggagc cacatacaca aggttgtttt tgatgaggca aaggtacggt
     1561 aggatccaca aaaaaccatc gctcatcaat ttgaacaaaa agagaggttt cgtggtgtcc
     1621 ctgtatcccc tcatctgtat caaaaaaggc tttaaattca acctgactat gtattctcga
     1681 aagagaaggt aagtgattta gcacggataa tcctacccat tttgtatttt cagcccactc
     1741 tttcattgca gtctgatcaa gtaatttctg ctgacttggc actgtcgttt ccacaatata
     1801 gggaatatta accaaagtat aagcactgta acgggagcgc attaattgct ctgccgttga
     1861 tgcaaatgtc tgttttaaat ggaaaggttg gcaacattca gcgtagcgtt tgcctgtttg
     1921 gcaaggacaa gcggtgattt tttctaacat ttttacattt ccgcataaaa ataaagatgt
     1981 gaaaagttta cgctataaat aaagaaaaat aaattttata aaaaagcgtg acatacccct
     2041 tgtggataac ttatgcaaaa atcgtgaatt ttttgggtat tcattgcttt aaaaagcc
//
LOCUS       DANLXS010000273         2074 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.281, whole genome shotgun sequence.
ACCESSION   DANLXS010000273 DANLXS010000000
VERSION     DANLXS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.281"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(88..1803)
                     /gene="proS"
                     /locus_tag="PXD71_002596"
     CDS             complement(88..1803)
                     /gene="proS"
                     /locus_tag="PXD71_002596"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL6292653.1"
                     /translation="MRTSQYLFSTLKEMPNDAQVVSHQLMLRAGMIRPMASGLYNWLP
                     TGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELWVESGRWNDYGPELLRFKDRGER
                     DFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
                     YSFHTTQESLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGE
                     DDVVFSTESDFAANIELAEAVAVGERQAPTAEMQLVDTPNAKTINELVENHGLAIEKT
                     VKTLVVKGATEEQPLIALVIRGDHELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVG
                     SLGVVNMPIPVIIDRSVALMSDFGCGANIDGKHYFNVNWERDVAVPEIADLRNVVEGD
                     PSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVV
                     AAAIEQHYDDRGIIWPCDEMAPFTVAIVPMNMHKSEKVQAFAEELYANLKAQGVEVIF
                     DDRKERPGVMFADMELIGVPHMVVIGEKNLENGEIEYKNRRSGEKQMIAKESLLAFLK
                     ANVKA"
BASE COUNT          545 a          519 c          373 g          637 t
ORIGIN      
        1 aataatagag tattttatac tttagttaaa tactcgctgc tttatattaa agggcgtatt
       61 ttatacgccc aattttgtta tttataatta agcctttaca ttcgctttta agaacgcaag
      121 taagctctct ttggcgatca tctgtttttc accgctgcgg cggtttttgt attcgatttc
      181 gccgttctcg aggtttttct caccaataac caccatatgc ggcacaccga taagctccat
      241 atccgcgaac atcacgcccg ggcgttcttt acgatcatca aaaatcacct caacgccttg
      301 agcttttaag ttagcgtata actcttcagc gaatgcctgc actttttccg atttgtgcat
      361 attcatcggc acaatcgcca ccgtaaatgg cgccatttca tcacaaggcc agataatacc
      421 acgatcatca taatgttgtt caatcgcagc tgccaccaca cgggtaacgc cgataccgta
      481 acaacccatt gtcatcacta gcggtttacc gtcttcacct tgcacagtgg ctttcattgc
      541 ttctgagtat tttttgccta attggaaaat atgccctact tcaatacctc gtttgatttg
      601 aagtgtgcct ttaccgtccg ggcttgggtc gccttccacc acattgcgta aatctgcaat
      661 ttccggcaca gcaacatcac gctcccagtt cacgttaaag tagtgtttgc catcaatatt
      721 cgcaccgcag ccaaaatctg acatcaacgc cactgagcgg tcaataatca ccggaatcgg
      781 catattcacc acacctaacg agccaacacc ggcaccgatt ttggctttaa tttccgcttc
      841 atcggcaaac tcaagcggat cagccaccaa cgggtgtttt tgagctttaa tttcgtttaa
      901 ctcgtggtcg ccacgaatca ctaacgcaat taacggttgc tcttcagttg cccctttcac
      961 cactaaggtt ttcaccgttt tttcaatggc taaaccgtga ttttctacta attcattgat
     1021 ggttttcgca tttggcgtat ccactaattg catttctgcc gttggtgctt ggcgttcacc
     1081 caccgcaacc gcttcggcta attcaatatt ggcagcaaaa tctgattcgg ttgaaaatac
     1141 cacatcatct tcaccgcttg aggctaacac ttggaactcg tgagaggctg aaccgccgat
     1201 agagccggta tctgcctgca ccgcacggaa atctaagccc aaacgggtga aaatattgct
     1261 gtaaacctga tacatcacat cataggtttc ttgcaggctc tcttgtgtgg tgtggaaaga
     1321 ataggcatct ttcatcacaa attcacgcga acgcattacc ccgaaacgag ggcggacttc
     1381 atcacggaat ttcgtttgaa tttggtataa attcagcgga agttgtttgt acgaagaaac
     1441 ttcacggcga accaagtcgg taatcacttc ttcgtgagtc gggccaagca cgaaatcacg
     1501 ctcgccacga tctttaaaac gtaataattc cgggccgtaa tcattccaac gaccggattc
     1561 cacccataat tccgccggct gaaccaccgg cattaatacc tcgatagccc cgcctttgtt
     1621 catctcttca cgaataatat tttccacttt tttcaacact ttgatcccgg tcggcaacca
     1681 gttgtataaa cctgacgcca tcgggcgaat cattccggca cgcaacatta attgatggct
     1741 aactacttgt gcatcattcg gcatttcttt aagggttgaa aataaatatt gacttgtacg
     1801 cattgctatt cctattcttt ttaaactttg aaacctgaaa aaatctgctt attttaaaac
     1861 aaagcggtta aattttcagg aaattttgca aaatcctata aaaaaataac cgcttgtaag
     1921 cggttatttt gttttaacta agggtatttt cttaaactgt gtatgcttaa ttttctcgtt
     1981 caatttgctc aataatctct tgccattcgg catttgcttg ttcaagagta aaaatctgac
     2041 ctttttttac tttagcctct aaatattcat cata
//
LOCUS       DANLXS010000274         2048 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.282, whole genome shotgun sequence.
ACCESSION   DANLXS010000274 DANLXS010000000
VERSION     DANLXS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.282"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            83..2014
                     /locus_tag="PXD71_002597"
     CDS             83..2014
                     /locus_tag="PXD71_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6292654.1"
                     /translation="MIFFTDLILKRGHSVLIDNSSVTIHTGQKVGLVGKNGCGKSSLF
                     ALLKNELQPEGGDVSLPKNWAISWVNQETPALAISALDYVIQGDREYTHLMAELDIAN
                     EKNDGNRIAIIHTQLDTIDAWTIQSRAATLLNGLGFSTEQLQLPVKSFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLERWLSNYRGTLLLISHDRDFLDPIIDRVIH
                     IEHQKLNEYTGNYTSFEIQRATKIAQQNAAYQQQQRKVAHLQSFIDRFKAKATKAKQA
                     QSRIKALEKMELIAPAYADSPFEFEFRPPLALPSPLLMMEKVSAGYAEKTVLESVKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELPPQNGSIQLAKGVQLGYFAQHQVDTLRFDE
                     SPLWHLQKLAPEKTEQELRNYLGGFDFKGDKVVQAVKSFSGGEKARLVLALIVWQRPN
                     LLLLDESTNHLDLEMRQALVDALTYYEGSLVVVSHDRHLLRSTVNEFYLVHDRKVEKF
                     KGDLDDYQKWLNEQNALANEPKSVLAKDSANSTACSSTNENSAAQRKEQKRLEAERRQ
                     QTAPLRKKITQLEKELEKATEKLNKLEAMLTESDIYEAENKAKLTETLAKQLEAKRLV
                     EEIEMQWLEAQEEFETMLR"
BASE COUNT          622 a          446 c          460 g          520 t
ORIGIN      
        1 ttcttatttt agttcacaaa gcaatggctt tgctttttta tgctaaaata cgcaaaattt
       61 ttaacaaaat agaccgcttg caatgatctt ttttacagac ttaattttaa aacgagggca
      121 ttccgttctc attgataaca gctccgttac cattcacacc gggcagaaag tcggtttggt
      181 gggaaaaaac ggctgtggca aatcctctct ttttgcctta ctgaaaaatg aattacaacc
      241 tgaaggtggc gatgtatcgt tgccgaaaaa ctgggcgatc tcgtgggtaa atcaagaaac
      301 gcctgcgtta gctatttcag cgttagacta tgtgattcaa ggagatcggg aatataccca
      361 tcttatggct gagctagata tcgcaaatga aaagaatgac ggcaaccgca ttgcgattat
      421 ccatactcaa cttgatacca ttgatgcttg gacaattcaa tcccgtgccg ctaccctgct
      481 taacggcttg gggtttagca ccgaacaact gcaactgccg gtgaaatcct tctccggtgg
      541 ctggcgtatg cggttaaatt tggcacaagc tctgatttgt cgttccgatc tgttattgct
      601 tgacgagcca accaaccact tagacttaga tgcggttatt tggttggagc gttggctgag
      661 taactatcgt ggcactttat tgttaatttc gcacgaccgt gattttctcg atccgattat
      721 cgatcgggtg attcatattg aacatcaaaa gctcaatgaa tacaccggca attacacctc
      781 gtttgagatt caacgtgcga ccaaaatcgc ccagcagaat gccgcttatc agcaacagca
      841 acgtaaagtt gctcatttgc agagttttat cgatcgcttt aaagccaaag cgactaaagc
      901 gaaacaggca caaagccgaa ttaaagcgtt ggaaaaaatg gagcttattg ccccagccta
      961 tgctgattcg ccgtttgaat ttgaatttcg cccaccgctt gcactgccta gcccgttatt
     1021 aatgatggaa aaagtcagtg ccggctatgc ggaaaaaacg gtgttggaat cggtcaaact
     1081 gaatttggtt cccggctctc gtattggttt actcgggaga aacggtgcag ggaaatccac
     1141 gctgattaaa ttattggcgg gcgaacttcc gccccaaaac ggctcaattc aactggcaaa
     1201 aggcgtgcaa ctcggctatt tcgcccaaca tcaggtggat acactacgtt ttgatgaaag
     1261 cccactttgg catttacaaa aactcgcacc ggaaaaaacc gagcaggaat tacgcaatta
     1321 tttaggcggt ttcgatttta aaggcgataa agtggtacaa gctgtgaaat cattctccgg
     1381 cggagaaaag gcccgtttag tgcttgcctt aattgtatgg caacgcccga atttactgct
     1441 attagatgag tcaaccaacc atttagattt agaaatgcga caagccctgg ttgatgcact
     1501 gacctattac gaaggctcgc tagtggtggt ttcgcacgac cgccatttat tgcgtagcac
     1561 cgtgaatgaa ttttatttag tgcacgaccg caaagtggaa aaatttaaag gtgatttaga
     1621 cgattaccaa aaatggctca atgagcaaaa cgccttagcc aacgagccga aatccgtact
     1681 tgcaaaagat tcggcaaatt cgaccgcttg ctctagcacc aatgaaaata gtgcagctca
     1741 acgcaaagag caaaaacgct tagaggcaga gcgccgccaa caaaccgccc cattgcggaa
     1801 aaaaattact caactggaaa aagagttaga aaaagcaacc gaaaaattaa ataaactcga
     1861 agcaatgctt accgaaagcg acatttatga agccgaaaat aaagcaaaac taactgaaac
     1921 acttgcgaaa cagttagagg caaaaaggct ggttgaagaa attgaaatgc aatggttgga
     1981 ggctcaggag gagtttgaga ccatgttacg ctagggttct acaagatagc gcaagcgtcc
     2041 acgcttgt
//
LOCUS       DANLXS010000275         2034 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.283, whole genome shotgun sequence.
ACCESSION   DANLXS010000275 DANLXS010000000
VERSION     DANLXS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.283"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(133..351)
                     /gene="ilvM"
                     /locus_tag="PXD71_002598"
     CDS             complement(133..351)
                     /gene="ilvM"
                     /locus_tag="PXD71_002598"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDL6292655.1"
                     /translation="MEEYQLTLKANKRPETLERILRVIRHRGFEVKCLNVESTDSAFN
                     LNFTLASKRPLSLLSHQLEKLFDIIEIN"
     gene            complement(361..2013)
                     /locus_tag="PXD71_002599"
     CDS             complement(361..2013)
                     /locus_tag="PXD71_002599"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDL6292656.1"
                     /translation="MNGARFITESLKAHGVTTLFGYPGGAIMPTYDAIYDSGLDHLLC
                     RNEQGAAMAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASH
                     LIGTDAFQEADVLGLSLACTKHSFIVQNIEELPEILALAFQIAQSGRPGPVLVDIPRD
                     VQLAQTSALPIVYPKEPADAQDPNKLVVAKQLLATAKRPVLYVGGGVSMADGVQAVRN
                     FVKIANIPSVSTLKGLGTIDPTDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDR
                     VTGKLDTFAPHAKVIHIDIDNAEINKLRQAQVALKGNLIEAVNYLAQPLDIEPWREDV
                     RNLKQQFDFQYQENSGDGNIDAVALLHTLSQRKAKNSIVTTDVGQHQMWSAQHLTYHD
                     PKNYITSAGFGSMGFGLPAAIGAIKARAEDQVILITGDGSIMMNIQELGTLKRADLAV
                     KILLLDNQRLGMVRQWQSLFFHGRHSQTILDDNPDFVLLAKAFDIDGERIEKASEVSD
                     AIDRMFNAKGAYLLHVCIPQEDNVWPLVPPGACNADMLDDEV"
BASE COUNT          586 a          444 c          428 g          576 t
ORIGIN      
        1 aaaataaaga gcaataagtg aaaaaaaaga gttgaaattt gaagaaatga ataaaatcaa
       61 aaggatagcg aatgataaat tttaagcata aaaaagccgt gtattttttt acacggcgaa
      121 tgaaaagtct gattaattga tttcaatgat atcaaacagt ttttctaatt gatggcttaa
      181 aagagagagt ggacgtttac ttgctaatgt aaaatttagg ttaaaggcgc tgtctgttga
      241 ttctacattt aagcatttca cctcaaaacc acggtgacgt ataacccgta aaatacgctc
      301 taaggtttca gggcgtttgt tagctttaag cgttaattga tattcttcca tcttattctc
      361 ctatacctca tcgtctaaca tatcggcatt acacgccccc ggcggtacca gtggccaaac
      421 gttatcttcc tgtggaatac aaacgtgaag taagtaagcc cctttggcat tgaacattct
      481 gtctattgca tctgacactt cgctcgcttt ctcaattcgc tcaccgtcaa tatcaaaggc
      541 tttcgccaac aacacaaaat cagggttatc atctaaaatg gtttggctgt gtcgcccgtg
      601 gaagaacagc gattgccatt ggcgaaccat acctaaacgt tggttatcca gtaataggat
      661 tttgaccgct aaatcagctc ttttaagcgt gccgagttct tgaatattca tcataatcga
      721 gccatcaccg gtaattaaaa taacctgatc ttccgctcga gctttgattg ccccaatcgc
      781 cgcaggcaag ccaaaaccca tagagccaaa accggcagag gtgatatagt ttttcggatc
      841 gtgataagtt aaatgttgtg ccgaccacat ttgatgctgc cctacatcgg tagtgacgat
      901 gctgtttttg gctttccgct gtgaaagcgt gtgtaataac gccacggcat caatattgcc
      961 atcaccacta ttttcttgat attgaaagtc aaattgctgc tttaaattac gcacatcttc
     1021 acgccatggt tcaatatcta acggttgagc caagtaattc acggcttcaa tcaaattccc
     1081 ttttaaggca acttgtgctt gacggagctt gttgatttcc gcattgtcga tatcaatgtg
     1141 aatcacttta gcgtgagggg caaaagtgtc taatttgccg gtaacacggt catcaaagcg
     1201 ggcaccacag gcaatgagta aatcacactc ttgtaccgcg taatttgctg cttttgtgcc
     1261 gtgcataccg atcattccca tatagagcgg atcggttgga tcgatagtgc ctaacccttt
     1321 taaggtagaa acagacggaa tatttgcaat ttttacaaaa tttctgaccg cttgtacacc
     1381 atcagccata ctaacgccac cgccgacata aagtaccggg cgtttggcgg tggctaataa
     1441 ttgtttggca accactaatt tgttgggatc ttgtgcatcg gcaggttctt taggataaac
     1501 gataggcaga gccgaagttt gagctagttg cacatcacgt ggaatatcga ccagcaccgg
     1561 accaggtctg ccactttgtg caatttggaa tgcgagggct aaaatttcag ggagctcctc
     1621 aatattctga acaataaaac tgtgtttagt gcaagcgagt gacaaaccta gtacatcagc
     1681 ttcttggaag gcatcagtgc cgattaaatg gctggcaact tgtccggtaa ttgctaccac
     1741 aggaatagaa tcaagtgcgg catcgcctaa accggtgatt aaattagttg ccccagggcc
     1801 ggaggtcgca atacaaaccc ctactttacc tgttgcacgg gcataaccga tagctgccat
     1861 tgcagcacct tgttcattgc ggcaaagtaa atgatctaag cctgaatcat aaattgcatc
     1921 ataggtaggc ataatagccc cgcccggata gccaaaaagt gtcgttaccc cgtgagcttt
     1981 taaactctcg gtgataaatc ttgctccatt catggatgtt gtgtccttaa tatt
//
LOCUS       DANLXS010000276         2034 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.284, whole genome shotgun sequence.
ACCESSION   DANLXS010000276 DANLXS010000000
VERSION     DANLXS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.284"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(55..1938)
                     /gene="htpG"
                     /locus_tag="PXD71_002600"
     CDS             complement(55..1938)
                     /gene="htpG"
                     /locus_tag="PXD71_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDL6292657.1"
                     /translation="MANNQETRGFQSEVKQLLQLMIHSLYSNKEIFLRELISNASDAA
                     DKLRFKALSEPALYEGDGELRVRVSVDETLGTITIADNGIGMTREQVIDHLGTIAKSG
                     TKEFLNALGTDQAKDSQLIGQFGVGFYSSFIVADKVTVKTRAAGVPAEQGVIWESAGE
                     GDYTVADLEKKERGTEIILHLREDEKEFLNEWRLREIIGKYSDHIGLAVEIQTNEYDD
                     EGKASGQKWEKINKAQALWTRSKNEISDEEYTEFYKHLSHDFADPLIWSHNKVEGKQE
                     YTSLLYVPSKAPWDLFQREQKHGLKLYVQRVFIMDDAEVFMPNYLRFMRGLLDTNDLP
                     LNVSREILQENKVTQSLRSALTKRALQLLEKLAKDDAEKYAQFWQAFGVVLKEGVGED
                     FANKQQVAGLLRFASTQRDDSEQAVSFADYIARMKEGQKAIYFLTADSYQAAKNSPHL
                     ELFHKKGIEVLLLSDRIDEWLVSHLTEFDGKPLQSITKSDLDLGELADKQEEESQKAQ
                     QAEFGSFLERAKGYFGERVKNVVLTHRLTDTPAVVSTDSDEMTTQMAKLFAAMGQQAP
                     EVKYTFELNPDHPMVKKVADLADEAEFADWIELLFEQALLAERGTLENPTAFIKRVNK
                     LLG"
BASE COUNT          475 a          497 c          470 g          592 t
ORIGIN      
        1 attatatatt gaatatggaa aaacggaaac ctagatttcc gtttttagta atggctagcc
       61 caacaattta tttacccgtt taataaaggc tgtcgggttt tccaacgtgc ctcgttcagc
      121 aagcagagct tgttcaaaca gcagttcaat ccaatcggcg aactcggctt catcggcaag
      181 atcagctact tttttcacca ttgggtgatc tgggttcagc tcaaaagtat atttcacttc
      241 tggtgcttgt tgccccattg ctgcaaacag ttttgccatt tgggtggtca tttcatcgct
      301 gtcagtggaa accactgccg gtgtgtcggt taagcggtga gtcagcacca cgtttttgac
      361 ccgttcacca aagtagcctt tcgcccgttc taagaacgaa ccaaactccg cctgttgggc
      421 tttttggctc tcttcttcct gcttgtcggc aagctcgcct aagtctaagt ccgacttggt
      481 aatgctttgt agcggtttgc cgtcaaactc ggtgaggtgg ctaaccaacc attcatcaat
      541 gcggtcagaa agcagtagca cctcaatgcc ttttttgtgg aacagctcta agtgcgggct
      601 gtttttagcc gcttggtagc tgtctgcggt taagaagtag atcgctttct gcccctcttt
      661 cattcttgca atataatcgg caaaactgac cgcttgttcg ctgtcatcac gctgggtgga
      721 ggcaaaacgg agtaagcctg caacctgttg tttgttggca aagtcttcgc ccacgccctc
      781 tttaagcacc acgccgaacg cttgccagaa ttgggcatat ttttcggcat cgtctttcgc
      841 caatttttcc agcagttgta acgcacgctt ggttaaggca gaacggagcg attgggtaac
      901 tttattctct tgcagaattt cacggctgac gttgagcggc aggtcgttgg tgtcgagcaa
      961 gccacgcata aaacgcaagt agttcggcat aaagacttcc gcatcgtcca taataaatac
     1021 acgctgaacg tagagtttca agccgtgttt ttgctcgcgt tggaacagat cccacggggc
     1081 tttgctcggc acatacagca agctggtgta ttcttgctta ccttcgactt tattgtgcga
     1141 ccaaatcagc gggtcggcaa aatcatggct gaggtgtttg taaaattcgg tgtactcctc
     1201 gtcactaatc tcattttttg agcgtgtcca aagcgcttga gctttgttaa ttttttccca
     1261 tttttgaccg cttgctttgc cctcgtcatc gtattcattg gtctgaattt ccaccgccag
     1321 gccgatatgg tcggagtatt tgccgataat ctcacgcaaa cgccattcgt ttaaaaactc
     1381 tttttcgtcc tcacgcaagt gaagaatgat ttccgtgcca cgctcttttt tctccagatc
     1441 ggcaacggtg taatcgccct caccggcaga ttcccaaatc acgccttgct cggcaggcac
     1501 tccggcggca cgggttttga ctgtcacttt atccgccacg atgaaagaag agtaaaagcc
     1561 cacgccgaac tgcccgataa gctggctgtc tttggcttga tcagtgccga gtgcgttcaa
     1621 aaattctttg gtaccagatt ttgcaatcgt accgagatgg tcgatcacct gctcacgggt
     1681 cataccgata ccgttatcgg caatcgtaat cgtaccgagt gtttcatcaa cagagacacg
     1741 cacacgcagt tcgccatcac cttcatacaa agccggctcg gaaagggctt taaaccgcaa
     1801 tttatcggcg gcgtcagagg cgttggaaat aagctcacgc aggaaaattt ctttgttgga
     1861 gtaaagcgag tggatcatca gttgcaataa ctgtttcact tccgactgga agccacgggt
     1921 ttcttgatta tttgccattg tgtattcctt tattcattaa aaaacaggga atagatgggg
     1981 caagtggtca gattttcaag attttttgca aaatatctgt tgccaaatag cgaa
//
LOCUS       DANLXS010000277         1957 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.285, whole genome shotgun sequence.
ACCESSION   DANLXS010000277 DANLXS010000000
VERSION     DANLXS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.285"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..664
                     /locus_tag="PXD71_002601"
     CDS             <1..664
                     /locus_tag="PXD71_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6292658.1"
                     /translation="VFDGNKNRFIPCENQAEDKANYFEISDLDYIKAEAQGELVAVVH
                     SHPEPNGKPVLSTLDRKMQVQTGLDWWLVHNQKIHKFRNVPHLIGREFKHGTMDCYTL
                     YRDAYMLAGYEMDEFERQDDWWHTGQNLYLDNIQGQGFERVETPQIGDVILMQVGADV
                     PNHAAIYIGDQMVVHHSPNRLSKRDLYDGYWLRHTHSIWRHKLAEKLDFDGILNDIAV
                     NN"
     gene            933..1562
                     /locus_tag="PXD71_002602"
     CDS             933..1562
                     /locus_tag="PXD71_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL6292659.1"
                     /translation="MITVKFYGHLKTFGTDFKILVKDIAEAIRALCSQLKGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVVKGAKSGGIFQAVLGVALIGAA
                     FALGPIGLGLVGSATAMTVGAMGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            1572..>1957
                     /locus_tag="PXD71_002603"
     CDS             1572..>1957
                     /locus_tag="PXD71_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292660.1"
                     /translation="MRIYGAKGGGGSSHTPIEAPETGRSKQIVNIVELLCEGEIEGLV
                     DGFKSIYLDGTQIQNDDGTYNFNNVSGQLNVGTQDQDVLDGYDSSQNEVNVGVEIKKK
                     NGAIVRTVTDERINRLRLTLGVRSLF"
BASE COUNT          597 a          379 c          467 g          514 t
ORIGIN      
        1 tgtttttgac ggcaataaaa atcgatttat cccttgcgaa aaccaagcgg aagacaaagc
       61 caattacttt gaaatctctg atcttgatta catcaaagca gaagcacaag gcgagctggt
      121 ggcggtagtt cattcacacc ctgagccaaa cggcaagccg gtactctcta cccttgatcg
      181 caaaatgcaa gtacagaccg gtttagattg gtggctggta cataatcaaa aaatccataa
      241 attcaggaat gtgccgcatt taatcgggcg tgagtttaaa cacggtacaa tggattgtta
      301 cacgctctac cgtgatgcct atatgcttgc cggttacgag atggacgagt tcgagcgaca
      361 agatgactgg tggcataccg ggcaaaatct ctatttggat aatattcaag ggcaaggctt
      421 tgagcgagtg gaaaccccac aaatcggcga tgtgatttta atgcaagtag gtgctgatgt
      481 gccgaaccac gctgccattt acattggcga tcagatggta gtccatcata gccccaaccg
      541 attatctaag cgtgatttat acgacggcta ctggttacgc catacccaca gtatttggcg
      601 gcataaactg gcggagaagt tagattttga tggcatattg aatgatattg ctgtaaataa
      661 ctaacaaaaa atgaccgctt gtaacaaaaa caaaccccga agcgtttgca gcacttcggg
      721 gtttttcata tccacttacc taactaagag gacataaatt aaatgaatga taaacgattt
      781 acattcaaat ttctaggagt tcttatggaa gcaataaata ttaccccaaa agaaatcaga
      841 aaaacaatgt ggacagccgc aataattctc tttttgtttg cgttcatctg gaaagcccct
      901 gaatttatca ctgctattcg ttggtggtaa ctatgattac agttaaattt tacggacacc
      961 ttaaaacatt cggcacagat tttaaaattt tagttaaaga catcgctgag gcaattcgtg
     1021 ctctgtgttc acagcttaaa ggattgcgtg aagcgttgcg agacggtgtt tataaagtcc
     1081 gtatcggtaa gcagtattta gacccatcag cccttgaaaa agggcttttt tattgcctga
     1141 agaaagggca aaccattcat tttacccctg ttgtcaaagg agcgaaaagc ggtggtattt
     1201 ttcaagcggt gttaggtgtg gcgttaattg gggctgcatt tgcgttaggt cctatcggct
     1261 taggattggt tggctctgct acagcaatga cagttggagc aatgggggca tcaatgttac
     1321 taggaggagt ttcccaaatg ctcaccaaaa tgccaaaagc ccccacaatg ggtaatgaaa
     1381 cagaaaaaga aagctcaacg gctttttcaa atttaaataa cttggtggct caaggcaaac
     1441 ctgtgccgtt ggcttatggt ttgattcgta ccggctcgct ggtgatatcg cagggagttg
     1501 agacgatcac cattaaagaa aaccaaccgg caagcaacaa gaaaaccggc tttagaaaat
     1561 aagggataat tatgcgtatt tacggagcaa aaggtggtgg cggcagtagc cataccccga
     1621 ttgaagcacc ggaaaccggt cgctcaaaac agattgtgaa tattgtggaa ttgctgtgcg
     1681 agggcgaaat tgaaggcttg gttgatggct ttaaatcgat ttacctagac ggcacgcaga
     1741 tccaaaacga tgacggcacc tataatttta ataatgtgag cggtcagcta aatgtcggta
     1801 cgcaagatca agatgtgttg gacggttacg atagctcaca gaatgaagtg aatgtgggtg
     1861 ttgagattaa gaaaaagaat ggggctattg tccgcaccgt aactgatgaa cgaattaacc
     1921 gcttgcgttt aacgttggga gtaaggtcat tattcca
//
LOCUS       DANLXS010000278         1946 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.286, whole genome shotgun sequence.
ACCESSION   DANLXS010000278 DANLXS010000000
VERSION     DANLXS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.286"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1642
                     /locus_tag="PXD71_002604"
     CDS             <1..1642
                     /locus_tag="PXD71_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL6292661.1"
                     /translation="DLNADTSQAFLNELDQTLWTAADKLRKNLDAANYKHIVLGFIFL
                     KYISDSFTDFQAKLKTQLTTPESELYLDPALFDEQEFSQILAEELEQRDYYAAENIFW
                     VPEQARWDNIKSLSKLNLGDELPWGDKFKGVSRLIDDAFEAIERENPKLKGVLQRIAG
                     FGVPDEMLTGLIDLFSRTNFTQPMHNGEPVHLQAKDILGHVYEYFLGQFALAEGKKGG
                     QYFTPKSIVTLIVEMLEPYSGRIYDPAMGSGGFFVQADRFIQAHAGNRNAISVYGQES
                     NSTTRKLAVMNMAIRGIPFDFGDKPEDTLLNPLHIDKKMDVVMANPPFNQKEWWNESL
                     ANDPRWAYGTPPQGNANFAWLQHMIYHLSPKGKMALLLANGSMSSQTSGEGDIRKNIV
                     QADLVEAMIALPNQLFTNTQIPACIWIINKAKARKGEVLFINATQIGYLKDRVLRDFT
                     ADDIAKISDTYHNWQKQNGYENIPAFCYCATLDEIAKNDFVLTAGRYVGAVQEENDGV
                     PFAEKMQELTALLNEQFKQGRELEQQIAENLKGLGYGI"
     gene            1632..>1946
                     /locus_tag="PXD71_002605"
     CDS             1632..>1946
                     /locus_tag="PXD71_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6292662.1"
                     /translation="MAFNQYVFSDIVELISEKIKIKDLKKENYISTDNMLPNFGGITL
                     AENLPNSASCNRFAKKDILFSNIRTYFKKVWLAEFSGGCSPDVLVMRSKNTDILLNEY
                     LFL"
BASE COUNT          594 a          478 c          402 g          472 t
ORIGIN      
        1 tgatctcaac gctgacacaa gtcaagcctt tttaaacgaa ctcgaccaaa ccctctggac
       61 tgccgccgac aaactgcgta aaaacctcga tgccgccaac tacaaacaca tcgttcttgg
      121 ctttatcttc ctaaaataca tctccgacag ctttaccgat ttccaagcca agctaaaaac
      181 ccagcttacc acccccgaaa gcgaactcta tcttgaccct gcactatttg acgaacaaga
      241 atttagccaa attcttgccg aagagttgga acagagagat tactacgccg ctgaaaacat
      301 cttttgggtg ccggagcaag cccgctggga caacatcaaa tcattaagca aactcaatct
      361 tggcgatgaa ttgccttggg gagacaaatt taaaggtgtc agccgcttga ttgatgatgc
      421 ctttgaagcc atcgaacggg aaaaccccaa actcaaaggc gtactccaac gcattgccgg
      481 ctttggcgtg cctgatgaaa tgctcacagg cttaattgac ctgttctcac gcaccaattt
      541 cacccagcct atgcacaatg gcgaacctgt gcatctgcaa gccaaagaca ttttagggca
      601 cgtctatgaa tactttcttg ggcaatttgc ccttgccgaa ggcaaaaaag gcggtcaata
      661 cttcacgcca aaatccatcg ttaccctgat tgttgaaatg ctcgaaccct attcagggcg
      721 gatttacgac ccagctatgg gcagcggcgg cttttttgtg caagctgacc gctttattca
      781 ggctcacgca ggcaaccgca acgccatttc cgtttatggg caagaatcca actccaccac
      841 tcgcaaactg gcggtgatga atatggcgat tcgtggtatt ccctttgact ttggcgacaa
      901 gcccgaagat accctactaa accctttgca catcgacaaa aaaatggatg ttgtgatggc
      961 aaatccgccc tttaaccaaa aagagtggtg gaatgaaagc ctagcaaacg atccacgctg
     1021 ggcatacggc acaccgccgc aaggcaacgc caactttgcg tggttgcaac atatgattta
     1081 ccacctctcc cccaaaggca aaatggcact cctgctcgcc aacggctcaa tgagcagcca
     1141 aacttcaggc gaaggcgaca ttcgcaaaaa catcgtgcaa gctgaccttg tcgaagcgat
     1201 gattgccctg cccaatcagc tattcaccaa cacccaaatc cctgcctgca tttggattat
     1261 caataaagcc aaagccagaa aaggtgaagt gctgtttatc aacgccaccc aaataggcta
     1321 cctgaaggac cgcgtcttgc gtgattttac cgctgatgac atcgccaaaa tcagcgacac
     1381 ctaccacaac tggcaaaaac agaacggcta cgaaaatatc cctgcgtttt gttattgtgc
     1441 cacgctggac gaaatcgcca aaaacgattt tgtgctgacc gcagggcgat atgtcggtgc
     1501 ggtacaagaa gaaaatgacg gcgtgccgtt tgcagaaaaa atgcaggaat tgaccgcttt
     1561 attgaatgaa caatttaaac aagggcggga attggaacag caaatcgcag agaatttaaa
     1621 ggggttggga tatggcattt aatcagtatg tattttcaga tattgttgaa ttaatatccg
     1681 aaaaaataaa aatcaaagac ttaaaaaaag aaaactatat ttcgacagat aatatgctgc
     1741 ctaattttgg tggaataaca cttgctgaaa accttccaaa ttcagcttct tgcaataggt
     1801 ttgccaaaaa agacattttg ttttccaata taagaaccta ctttaaaaaa gtttggcttg
     1861 ctgaattttc aggtggttgt tctcctgatg ttctggtaat gcgcagtaaa aacacagata
     1921 ttttattaaa tgagtattta ttttta
//
LOCUS       DANLXS010000279         1895 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.287, whole genome shotgun sequence.
ACCESSION   DANLXS010000279 DANLXS010000000
VERSION     DANLXS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.287"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(114..404)
                     /locus_tag="PXD71_002606"
     CDS             complement(114..404)
                     /locus_tag="PXD71_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292663.1"
                     /translation="MNNLSEFKLRYINNPYSNKDTFFIFDELSILLKSLTSDELKKIG
                     TAKNLADRQLNKQRKRCKRYVSEAYRLVEDNKEPYSKFFNHDGITAYNIKAF"
     gene            complement(454..1218)
                     /locus_tag="PXD71_002607"
     CDS             complement(454..1218)
                     /locus_tag="PXD71_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6292664.1"
                     /translation="MPNITPKLVINALEPAKEKGIGDTLKRAIRLLNEVMNFAVNADV
                     IEFNKCQNVGSNFSVAKSEHNPTIHPKELPQFLAALRDYNLSFQTKMLIKWQLLTMTR
                     SREASETLWSEIDLEAKTWTISAERMKMKRPHVIPLSRQAVEILERMRKITGNSPFVF
                     QSEMKPRQPMNSQTANRAIGLLGYAGTLTAHGMRSIASTYLNEQLVNYDVVEACLAHV
                     IKDQTRKAYNRSDYLDQRVDVMQQWADYVESCSTGI"
     gene            complement(1187..1666)
                     /locus_tag="PXD71_002608"
     CDS             complement(1187..1666)
                     /locus_tag="PXD71_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDL6292665.1"
                     /translation="MARVTTELNNTQIKNAKAQTKDYKLMDGKGLFLLVKKNGSKLWR
                     FRYKKPITGKESDLGIGSYPETTLAQARTIREEYRALLAENIDPLEHRQRLEAEALAE
                     IENTFYKVCLKWRDKCESRVKNGELQALTLKKNWRILEKYLLPCRLLYSKHHAKTSY"
BASE COUNT          522 a          360 c          356 g          657 t
ORIGIN      
        1 tttaaatgta aattatatgc ttcataattt catattttat gggttttgtg attgtgatca
       61 taatacacat taaagaggtt tcaggacgat ataaaaaagg ctctctaaat agattagaaa
      121 gccttgatat tgtatgcggt tatcccatcg tggttgaaaa acttagagta aggttcttta
      181 ttatcttcaa caagacgata agcctctgat acataccttt tacatctttt tctttgctta
      241 ttaagttgtc tatctgccag atttttagct gttcctattt tctttaattc atcggaagtc
      301 agtgatttta atagtatgct taattcatca aatataaaaa atgtatcttt attagaataa
      361 gggttattga tatatcgtaa tttaaactca gaaaggttat tcatatttgt acacctctaa
      421 ttaaacaagc ggtcaaattc cgccaaaaat ttactaaatc cccgttgagc agctttctac
      481 ataatccgcc cattgctgca ttacatctac acgctgatct aaataatcag aacggttata
      541 agcctttcgg gtttggtctt tgataacgtg agctaaacaa gcctctacta catcataatt
      601 gactaactgc tcattcaaat aggttgaggc aatagaccgc ataccgtgag ccgttagagt
      661 gccggcatag cctaataagc ctatggctct gttggcggtt tggctgttca tcggttggcg
      721 tggtttcatt tcgctttgaa aaacaaatgg gctattccct gtgatttttc gcattctttc
      781 taaaatttcg accgcttgcc gtgaaagagg gataacgtga gggcgtttca tcttcattct
      841 ttcggctgaa atggtccacg ttttcgcctc taaatcaatt tcactccaca acgtttcgct
      901 tgcctctcgt gagcgtgtca ttgttaatag ttgccattta atcaacatct tagtttgaaa
      961 tgataggttg taatcacgca atgcagctaa aaattgaggt agctcttttg ggtgtatcgt
     1021 tgggttatgt tcagatttcg ctacgctaaa atttgagcct acattttggc atttgttaaa
     1081 ctcgataacg tccgcattta ccgcaaaatt catcacttca ttaagtagcc taattgctcg
     1141 tttaagggtg tcgcctatgc ctttttcttt agcaggctct aaagcattaa taactagttt
     1201 tggcgtgatg tttggaatag agtagtcggc aaggcaaaag atacttttca aggattcgcc
     1261 aattcttctt cagcgttaag gcttgaagtt cgccattttt tacacggctt tcgcatttat
     1321 cacgccattt caagcagact ttataaaagg tgttttctat ttcggctaac gcttcagcct
     1381 ctaatctttg gcggtgttcg agtgggtcga tattttccgc tagtaatgcc ctatattctt
     1441 ctctaatcgt tcttgcttgg gctagggtag tttcggggta actgcctatg cctaaatcgc
     1501 tctctttgcc ggtaatcggc tttttatacc taaaccgcca taacttcgag ccgtttttct
     1561 taaccagcaa aaacagcccc ttgccgtcca ttagcttgta gtctttggtt tgagcctttg
     1621 cgtttttgat ttgagtgtta tttaattctg tggtaactct agccataaaa atttacctaa
     1681 aatcgcattg tgctttatca gaatcataag ggatttaagg gtatgggttt catacccttt
     1741 tacgttatac cctcaaatat acccttaaaa taaggggatg taaacgcaag aacacgaaag
     1801 aatacgaatg aagaaataat ttaaaattgc tctaaagcca gtatcatcaa tggttttgcg
     1861 taagaatgcg aatgaagaaa taagagtgga aaatt
//
LOCUS       DANLXS010000280         1865 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.288, whole genome shotgun sequence.
ACCESSION   DANLXS010000280 DANLXS010000000
VERSION     DANLXS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.288"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(190..792)
                     /locus_tag="PXD71_002609"
     CDS             complement(190..792)
                     /locus_tag="PXD71_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL6292666.1"
                     /translation="METLIEFFSSYGYWAVFLVLLACGFGVPIPEDITLVSGGVISGL
                     GYTNVHWMLVVSMLGVLVGDSTMYWLGRIYGEKILKFPLIRNVATPERFATVQERFEK
                     QGWKLLFMARFLPGLRAVVYLVSGITRKVSFVKFLLVDFCAAIISVPIWVYLGDYGAS
                     NLDWLKEQVANGQHLIFAILGIVILFLGWKWYKNKKQYKK"
     gene            complement(794..1813)
                     /gene="purR"
                     /locus_tag="PXD71_002610"
     CDS             complement(794..1813)
                     /gene="purR"
                     /locus_tag="PXD71_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDL6292667.1"
                     /translation="MATIKDVAKYAGVSTTTVSHVINKTRFVAEDTTKAVWEAIKALN
                     YSPSAVARSLKINTTKSIGMIITTSESPFFAEIVLAVEEFCYSKGYSLFLCNTQNKLE
                     KIQNHLDMLIKKRVDGILVMCSEYTANSFDIFENSNIPMVIMDWGRSDERSDIILDHG
                     VEGGYIATQHLIENGHQDIAVITGNRDKEIARTRFEGFKKALSEAGLSIREEWIQEGD
                     FEPEGGYECMNNLLKSSEKLPTAVFCFNDSMALGAISAITEKGLNVPQDISVIGYDNV
                     HSSRFYAPPLTTIHQSKSRLGSAALELLLERIEDNENAKEPKVLEFFPELVKRKSVRN
                     LNIKE"
BASE COUNT          581 a          405 c          284 g          595 t
ORIGIN      
        1 aattctcttt tttgtctttt taatacttac tgttatttcg ttcttcttca agaaaaagtg
       61 acacagttaa acctaatttt ttaagttaaa atatagttca aaaaaacagt tggtaatgga
      121 tatttataat tattcttacc aactgttttg ctattatatc tttttaagat gaaatgctcg
      181 tcttttgttt cactttttat actgcttttt atttttatac catttccagc ctaaaaatag
      241 aataacaatg cctaaaatgg caaaaatgag gtgctgccca ttagctactt gttcttttag
      301 ccaatccaaa ttagatgccc catagtcgcc taaataaacc caaattggca cagagataat
      361 agcggcacag aaatcaacta ataaaaattt tacgaagctg acttttctgg taatacctga
      421 aactaagtaa accaccgcgc gcaagccagg taaaaaacga gccataaaaa gtagtttcca
      481 tccttgtttt tcaaaacgtt cttgcacggt tgcaaaacgt tcaggtgtcg caacattcct
      541 gattaatgga aatttgagaa ttttttcgcc atagattcta cctagccagt acattgtgct
      601 atcgccaact aatactccta acatactaac aaccagcatc caatgcacat tggtgtagcc
      661 taatcctgaa ataacaccac cggatactaa cgtaatatct tcaggaatcg gcacaccgaa
      721 accacaggca agtagcacaa gaaaaacagc ccaataacca tagctactaa aaaattcaat
      781 caatgtttcc atattactcc ttaatgttta aattccgaac cgatttacgt ttgaccaatt
      841 caggaaagaa ctctaatact ttaggctctt ttgcgttttc gttatcttca atacgttcta
      901 ataataattc taatgctgcc gagcctaagc gagattttga ctgatggatt gtcgtaagag
      961 gcggtgcata aaagcgtgag ctatgcacgt tatcataccc gatcactgaa atatcttgcg
     1021 gcacattcag ccctttttcg gtaatagctg aaattgcccc tagtgccatt gagtcattaa
     1081 aacaaaaaac cgcggttggt aatttttcac tgctcttaag caaattattc atacactcat
     1141 accccccctc aggttcaaaa tcgccttctt gaatccattc ttcacgaata gacaaacccg
     1201 cctcagatag cgctttttta aaaccttcaa aacgggttct tgcaatttct ttatctctat
     1261 tgccggtaat tacagcaata tcttgatgcc cgttttcaat taaatgctga gtcgcaatat
     1321 agccaccttc aacaccgtga tctaaaataa tatcactgcg ttcatcactt cgtccccaat
     1381 ccataatcac cattgggata ttgctgtttt caaagatatc aaatgaatta gctgtatatt
     1441 cggagcacat caccaaaatg ccatcaaccc gttttttgat gagcatatct aaatgatttt
     1501 gaattttctc aagtttgttt tgagtattgc acaaaaatag tgaataccct ttgctatagc
     1561 aaaattcctc taccgctaac acaatttcag cgaaaaacgg tgattcgctg gttgtaatga
     1621 tcataccgat agatttagtc gtattaattt tcaaactccg cgctacagca ctaggagaat
     1681 agttcagcgc tttaatagcc tcccaaactg cttttgtagt gtcctctgct acaaagcggg
     1741 ttttgtttat aacgtgagaa accgttgtag tcgatacacc agcatatttt gcgacatctt
     1801 ttatcgttgc catacctttt tcctcctaaa taagtagtgg tgcattataa ctgattctaa
     1861 ctgac
//
LOCUS       DANLXS010000281         1851 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.289, whole genome shotgun sequence.
ACCESSION   DANLXS010000281 DANLXS010000000
VERSION     DANLXS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.289"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            92..1042
                     /gene="coaA"
                     /locus_tag="PXD71_002611"
     CDS             92..1042
                     /gene="coaA"
                     /locus_tag="PXD71_002611"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL6292668.1"
                     /translation="MSLEKTSKITPFLTFDRQQWADLRKSVPLKLTEQDLKPLLGFNE
                     DLSLEEVRTIYLPLARLIQYYIEENLKRQTVLHRFLGIEPPKVPYIISIAGSVSVGKS
                     TSARILQALLSHYPKERKVDLITTDGFLYPLATLTEKNLLKKKGFPESYDIHSLIRFV
                     SDIKSGKRNVKAPIYSHLTYDIIPNQFNTVDQPDIVILEGLNVLQSGMNYPSSPHNVF
                     VSDFVDFSIYVDAEESLLKKWYINRFLKFRRSAFADPNSYFHHYASLSEQEAIETAST
                     IWEEINGLNLRQNILPSRERANLILIKGQDHAIETVKLRK"
     gene            1195..1734
                     /locus_tag="PXD71_002612"
     CDS             1195..1734
                     /locus_tag="PXD71_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HDL6292669.1"
                     /translation="MNKKFLLPLILFLALSVAFLVQLARNSQGDDPKKLESALIGKTV
                     PSFNLESVLDEKQTIDQNIVKTGKPRLLNVWATWCPTCYAEHEYLTILAKQGVEIVGI
                     DYKDERPKAIKFLANYGNPYKAIIFDPKGSLGLDLGVYGAPETFIIDGNGVIQYRHAG
                     DVNDQVWNQVLKPIYDGLK"
BASE COUNT          585 a          380 c          331 g          555 t
ORIGIN      
        1 acggcaaaat aagtgcatat attgtatagt tacgccctta atttggtatt taattacctt
       61 aatattccag caaaaaattg agattttggc tatgagcctt gaaaaaacca gcaaaattac
      121 tccttttctc acttttgatc gccaacaatg ggctgattta cgcaaatccg ttccattgaa
      181 gctgaccgag caagacctaa aaccgctttt aggctttaat gaagatcttt cattagaaga
      241 agtacgcact atttatttac cgcttgcccg tttaattcaa tactatatag aagaaaactt
      301 aaaacgccag acagtcttac atcgctttct cggcattgaa ccgccaaaag tcccttacat
      361 tattagtatt gccggcagcg tatcggttgg gaaaagtacc tctgcccgta ttctgcaagc
      421 tctgctctcc cattacccta aagagcgaaa agtggattta attaccaccg acggtttttt
      481 atatccactt gcaacgctaa ccgaaaagaa cttactcaag aaaaaaggct ttccggaatc
      541 ttatgatatt cacagcctga ttcgctttgt atctgatatt aaatccggca aacgcaatgt
      601 gaaagctccg atttattcac atttgaccta cgatattatc ccaaatcaat ttaatacggt
      661 ggatcaaccc gatattgtga ttttagaagg gctaaacgtg ttacaaagtg gaatgaacta
      721 cccgtccagc ccacataatg tatttgtatc ggactttgtg gatttttcta tttatgtgga
      781 tgccgaagaa agtttgctga aaaaatggta tatcaaccgt ttcttgaaat tccgccgcag
      841 tgcctttgcc gacccaaact cctatttcca ccattatgcg agtttgtccg agcaagaagc
      901 gattgaaacc gcctcaacga tttgggaaga gatcaacggc ttaaatttac gccaaaatat
      961 tctcccaagc cgagagcgtg ccaacctcat tttaatcaaa gggcaagacc acgccattga
     1021 aacggtaaaa ttacgcaaat aatattttgc aaataattgg gctttttcga ccgcttgtaa
     1081 taccttaaaa tccgttgaat accactcaac ggatttttta tttaaatcaa tgcttattca
     1141 actatttttt ggtaagctaa cgactttgag agtgattgat attaggcata tacaatgaac
     1201 aaaaaatttc tacttccttt aattctcttt ttggcgttat ctgtggcatt tttggttcaa
     1261 ctcgctcgca actcgcaagg cgatgatccg aaaaaattag aatcagcctt aattggcaaa
     1321 acagttccga gctttaattt agaatcggta ttagatgaaa aacagacaat cgaccaaaat
     1381 atcgtcaaaa ccggcaaacc tcgcctgctt aacgtgtggg caacttggtg tccgacttgt
     1441 tatgccgaac acgaatactt aaccattttg gcaaaacaag gggtggaaat tgtcggtatt
     1501 gattataaag acgaacgccc aaaagcgatt aaattcttag ccaattacgg caacccttat
     1561 aaagcgatta tttttgaccc gaaaggctca ctcggtttag atttaggtgt ttatggtgca
     1621 cccgaaacct ttattattga cggcaacggc gtgattcaat accgccacgc cggcgatgta
     1681 aacgaccaag tgtggaacca agtgcttaaa ccgatttatg atggattaaa gtaaatgttt
     1741 gtatttcctg ctgacattaa gaaatctata aaaagcaatt atcaaagaga atataacttt
     1801 atttttataa tagttggttt atcaactatt ataggaataa ttctaactat t
//
LOCUS       DANLXS010000282         1821 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.290, whole genome shotgun sequence.
ACCESSION   DANLXS010000282 DANLXS010000000
VERSION     DANLXS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.290"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            48..689
                     /gene="sodA"
                     /locus_tag="PXD71_002613"
     CDS             48..689
                     /gene="sodA"
                     /locus_tag="PXD71_002613"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDL6292670.1"
                     /translation="MAYTLPELGYAYDALEPHFDAKTMEIHHSKHHQAYINNANAALE
                     AHPELLEKCPGALIKDLSQVPAEKRIAVRNNVGGHVNHTLFWKGLKTGTTLQGALKEA
                     IERDFGSVEAFQSEFEKAATTRVGSGWAWLVLEEGKLAVVSTANQDNPLMGKEVAGVS
                     GYPILVLDVWEHAYYLNYQNRRPDFIKAFWNVVNWDEAARRFEEKVATCGCAK"
     gene            complement(752..1783)
                     /gene="alr"
                     /locus_tag="PXD71_002614"
     CDS             complement(752..1783)
                     /gene="alr"
                     /locus_tag="PXD71_002614"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL6292671.1"
                     /translation="MIKANAYGQGLLPAAGTLADMVDGFGVARLREALEIQETGYTGK
                     IVLVEGFFDREELLKTLSRKFDTVIHNLEQLELLEQVAKEWQAEQQKGFWTRKAKIYF
                     PINIWLKIDTGMHRLGIHPEQVDHFVERLKNCALVNSISFVSHFSRADELDCGYTEKQ
                     IAVFEQATQKYQDHDRSISASSGILYWKQAHYDWVRPGIIMHGISPHYTPITDLGFKP
                     VMTLSSSLIAVRTHKAGEPVGYGGAWVSPKDTKLGVTAMGYGDGYPRNAPEGTPVLIN
                     GRIVPIVGRVSMDMLTVDLGAESQDKVGDKAIFWGEQLLIEDVAKHIGVISYELITKL
                     TPRVIFEYK"
BASE COUNT          527 a          401 c          393 g          500 t
ORIGIN      
        1 ggagtttaac tctgattaac ctatttacta tttgagagga attaactatg gcatatacat
       61 taccagaatt aggctacgct tatgatgcgt tagagccaca tttcgatgct aaaacaatgg
      121 aaatccacca ttctaaacat caccaagctt acatcaacaa tgcaaatgca gcattagaag
      181 ctcaccctga attattagaa aaatgtccgg gtgcattaat caaagatttg agccaagtgc
      241 ctgcagaaaa acgtattgca gtgcgtaaca acgtaggtgg tcatgtaaac cacactttat
      301 tctggaaagg tttaaaaaca ggcactacct tacaaggtgc attaaaagaa gcgattgagc
      361 gtgatttcgg ttcagttgaa gctttccaat cagaatttga aaaagcagcg actactcgtg
      421 ttggttcagg ctgggcgtgg ttagtattag aagagggtaa attagccgtt gtttcaaccg
      481 ctaaccagga caacccacta atgggtaaag aagtggcagg cgtttcaggc tatccgattt
      541 tagtcttaga cgtttgggaa cacgcttact acttaaacta ccaaaaccgc cgtccggatt
      601 tcattaaagc attctggaac gttgtaaact gggacgaagc cgctcgccgt tttgaagaaa
      661 aagtagcaac ctgcggttgt gcgaaataat attttcgatc ttgaataaga tacaagcggt
      721 aaaatttgtt gaaaatttta ccgcttgttg tctatttata ttcaaaaatc acacgaggag
      781 tgagtttagt aatcagctca tagctaatca cgccaatgtg tttcgccaca tcttcaatta
      841 atagttgctc tccccaaaaa atggctttat cgcccacttt atcttggctc tctgcaccta
      901 aatcgaccgt cagcatatcc atcgaaactc gaccgacaat cggcactatt ctaccgttaa
      961 tcaacacagg ggtaccttca ggagcattgc gagggtagcc atcgccgtag cccattgcag
     1021 tcacgccgag tttggtatct ttagggctta cccaagcacc accatagcct acaggctcgc
     1081 cggctttatg ggtacgcaca gcgatcaggg aagaggagag cgtcattacc ggcttaaaac
     1141 ctaaatcggt aatcggcgta tagtggggag aaatgccgtg cataataata cccgggcgaa
     1201 cccaatcata atgagcctgt ttccaataaa gaataccgct tgaggcggaa atactgcggt
     1261 cgtgatcctg atatttttgc gtagcttgct caaacaccgc aatttgtttt tcggtatagc
     1321 cacaatcgag ttcatcggca cggctaaaat gactcacaaa gctgattgaa ttgaccaagg
     1381 cacaattttt caaacgttca acaaagtgat cgacttgctc cgggtgaatg cctaagcggt
     1441 gcattccggt gtcgattttt agccaaatgt taatcgggaa gtagattttc gctttacgag
     1501 tccaaaagcc tttttgttgc tctgcctgcc attcttttgc tacttgctcg agtaattcca
     1561 gttgttcgag gttatgaatg acggtatcaa acttgcggga aagggttttg agtagctctt
     1621 ctcggtcaaa aaaaccttca actaacacaa ttttgcctgt gtatccggtt tcttgaattt
     1681 ctaaggcttc acgcaaacgg gcaactccaa agccatcaac catatctgct aatgtgccgg
     1741 cagccggcaa taagccttgc ccgtaagcat tggctttgat catcgccaat aatttttgct
     1801 gtggagcgaa agatttgatc a
//
LOCUS       DANLXS010000283         1782 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.291, whole genome shotgun sequence.
ACCESSION   DANLXS010000283 DANLXS010000000
VERSION     DANLXS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.291"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(61..477)
                     /gene="tnpA"
                     /locus_tag="PXD71_002615"
     CDS             complement(61..477)
                     /gene="tnpA"
                     /locus_tag="PXD71_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL6292672.1"
                     /translation="MKKETEIRRGRHVVFNLHVHLVFVTKYRREVFTQAILNDLHSIF
                     ATICADFEATLVEFDGEDDHVHLLVEYPPKVAISHLVNSLKGVSSRMIRKKNYPSIRK
                     KLWGNQLWSPSYFAGSCGGAPISIIRQYIEQQQTPD"
     gene            524..1660
                     /locus_tag="PXD71_002616"
     CDS             524..1660
                     /locus_tag="PXD71_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6292673.1"
                     /translation="MLLRKAFKFEIRPNGEQVRKMKQFCGCSRFVFNKALAWQNEQYE
                     QDNHHKFSYTKLANLLPQWKKELVWLKDCHSQVLQQSLKDLESAFKNFFQKRADLPKF
                     KKKGVKESFRFPQGCKIEQKNDRLYLPKIGWVRYRNSREIVGEVKNVTVSMKCGRFFV
                     SIQTEFKQEIPTHKGGEIGIDMGIARFATLSNGEFFEPLNAFKTHKGKLAKLQKQLKN
                     KVKFSKNWQKLKDKIGKLHHKIANCRKDFLHKISTQISKNHAMIYVEDLQVANMSKSA
                     KGTAEVHGKNVKQKSGLNRVILDQSWFEFRRQLDYKTQWLGGFLLAVPPQNTSRTCPC
                     CNYTAKENRQTQENFECVECGYTENADVVGAMNVLTRGQAIVQA"
BASE COUNT          607 a          348 c          372 g          455 t
ORIGIN      
        1 accacgtcct ttagggcgtg gatataaggt ataaacggca aagccgtttc aaatttctaa
       61 ctaatcaggt gtttgttgtt gctcgatata ttggcgaata atcgatattg gtgcaccacc
      121 acaactacca gcaaagtaag acggtgacca aagttgattt ccccataatt ttttgcgaat
      181 gctcggatag tttttctttc taatcattcg gctcgaaacg ccttttaaac tgtttacaag
      241 atgagaaata gcgactttag gcggatattc cacaagtaga tgaacgtgat catcttcacc
      301 atcaaattcc actaatgtgg cttcaaaatc agcacaaatc gttgcaaaaa ttgagtgtaa
      361 gtcgttcaaa attgcttgag taaaaacttc cctgcgatat ttagtcacga aaactaaatg
      421 aacgtgtaag ttaaaaacaa cgtggcgacc acgtctaatc tctgtttctt ttttcatagg
      481 ccaagtgtag aataagcaca atttttgtat tctaattcgt gcaatgttac tacgtaaagc
      541 ctttaaattt gaaatcagac caaatgggga acaagttcgc aaaatgaagc agttttgcgg
      601 ctgttctcgc tttgtgttta acaaggcatt ggcgtggcaa aatgagcagt acgaacaaga
      661 taaccatcac aaattcagtt ataccaagct cgccaacttg cttccgcaat ggaaaaaaga
      721 attggtatgg cttaaagatt gccattcaca agtgcttcaa cagtcgttaa aagaccttga
      781 aagtgcgttt aaaaacttct ttcaaaagcg tgcggatttg cccaaattca agaaaaaagg
      841 cgtaaaagag agtttccgtt ttccgcaagg ttgcaaaatc gagcagaaaa acgaccgctt
      901 gtatctgccg aaaatcggtt gggtgcgtta tcgcaacagt cgtgaaattg ttggggaagt
      961 aaaaaatgtc accgtttcaa tgaaatgcgg acgctttttt gtgagcattc aaacggaatt
     1021 taaacaggaa attcccacgc ataaaggcgg agaaatcggc attgatatgg gcattgctcg
     1081 ttttgcaacc ctttccaatg gagaattttt tgagccgtta aatgcgttta aaacccacaa
     1141 aggaaaattg gcaaaactgc aaaaacagct aaaaaataaa gtgaagttca gtaaaaactg
     1201 gcaaaaactg aaagataaaa tcggcaagtt acatcacaaa atcgccaact gccgaaaaga
     1261 tttcctgcat aaaatctcaa cgcaaatcag caaaaaccac gcgatgatct atgttgaaga
     1321 tttgcaagtg gcgaatatgt ccaaatcagc caaaggcaca gcggaagtac acggcaaaaa
     1381 tgtgaaacag aaatcaggct taaaccgagt gatattagac cagtcttggt ttgaatttag
     1441 acgacaactg gactacaaaa cgcaatggtt aggcggattt ttgctagcag ttcctccaca
     1501 aaataccagt cgtacttgcc cttgttgcaa ctatacagca aaagaaaatc gccaaacaca
     1561 ggaaaatttt gaatgcgtag aatgtggcta tacggaaaat gccgatgtag tgggagcaat
     1621 gaatgttctt acacgtgggc aagcaatagt ccaagcataa taaatatcag ggtaggacat
     1681 acccgaagcg tctatgaagt gaatctcaat agagatcagc agtagaaacc caccgagagt
     1741 agcccacagc ttgctgtggg agctagtagg aatcccctgc ct
//
LOCUS       DANLXS010000284         1765 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.292, whole genome shotgun sequence.
ACCESSION   DANLXS010000284 DANLXS010000000
VERSION     DANLXS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.292"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(215..>1765)
                     /locus_tag="PXD71_002617"
     CDS             complement(215..>1765)
                     /locus_tag="PXD71_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase/relaxase domain-containing protein"
                     /protein_id="HDL6292674.1"
                     /translation="VHDIGKVVVDIEITLKDGSRWFAWNGLPPQPYKFKYIKGRDYHL
                     HPVLGGFLANQLIPKSAFDWLGQYPEVISSLMYAMSGHNDKAGLLSEIVQKADQHSVT
                     LALGGDVNKLVQRPVNSLSKQLVLALRYLLEQKIKLNTPKGPADGWFTEEGLWLVSKT
                     AADNIRAYLLGQGISVPKDNPKLFDEMQSLGIVEESPAGGAIWSCRIQADSGWTPANT
                     FTLLRIKPEVAWENINDRPNYFAGKVKIVEQITEESNIETQSTLVDGTTKPAPLPSVQ
                     TGGIIASKEVEISQVETNSDNAPSSEIIINDENDLTSFVLDMFAPTESSEPSLDDISI
                     GKGQEIASMPSKQEVQDKSTTKKLKVTQSAVETPETLTLSELQLQNKENSITGDNFVK
                     WLKSGIATNKFAINKPTAKLHIVEDHLFLVTPVIFEMYLQETGQPYDKETINNLQYDF
                     QGLGIHSPKNTMTNGKPDTINFWKCDVIGPRKTSSLTGYLIKNTRLFFGDKVLLNNHC
                     LTLQKEET"
BASE COUNT          487 a          356 c          341 g          581 t
ORIGIN      
        1 ttccacgtta ctgctttgat tttatcgcca acaatttgat tacgccattc aatgagcata
       61 tcggtggtaa tgtcattggc atttaacttt ggataacgct ttttgataat tcggattacg
      121 ttgttgtagc tccgtttggt atccggacga agatagtgac gatagaaaaa aaaatcggtc
      181 tagtagcgaa tcaaaagaga tagaagttat cattttaagt ttcctctttc tgtaaagtta
      241 aacagtgatt atttaatagt accttatccc caaaaaagag acgggtattt tttatcaaat
      301 atcctgttaa agaggaagtc tttctcggtc caatgacatc acatttccaa aaattgatgg
      361 tatccggctt accgttggtc attgtgtttt taggtgaatg aatccctaat ccttgaaaat
      421 catactggag attattaatt gtttccttat cataaggctg tccagtctct tgcaaataca
      481 tttcaaaaat aacgggggta actaaaaaca ggtggtcttc aacaatatgg agcttggcag
      541 taggcttgtt aattgcaaat ttgttggtcg caatacctga tttgagccac tttacaaagt
      601 tatctcctgt aattgagttc tccttattct gtaactgtag ctctgatagt gttaatgttt
      661 cgggcgtttc tactgcactt tgtgttactt tgagcttttt agtggtagat ttatcctgta
      721 cttcttgttt ggatggcata cttgcaattt cctgtccctt accaattgaa atatcatcaa
      781 ggcttggttc ggaactttcc gtaggtgcaa acatatccag cacaaaggag gttaaatcat
      841 tttcatcatt tatgatgatt tctgaggatg gtgcattatc cgagtttgtc tctacctgag
      901 atatttcaac ttctttagag gcaataatgc cccctgtctg aacactcggt aatggagctg
      961 gtttagtggt cccatcgact aatgtagact gagtctctat attactttct tctgttatct
     1021 gctcgactat tttaactttt ccggcaaaat aatttggtcg atcattaata ttctcccaag
     1081 ccacttctgg tttaattctc aataatgtaa aggtatttgc tggcgtccaa cctgaatcag
     1141 cttgaatacg gcaactccaa attgctccac cagccggtga ttcttctaca ataccgagag
     1201 actgcatttc atcaaataac tttggattat ccttaggcac tgaaattccc tgccccagta
     1261 agtaagctcg gatattatca gcagcagttt tgctaacaag ccacaatcct tcctcagtga
     1321 accaaccgtc tgccggacct ttgggtgtat ttaactttat tttctgttcc agcaaataac
     1381 gtagtgccaa gaccagttgc tttgacagag aattgacagg acgttgaacc agtttattta
     1441 catcgccacc gagtgcgagg gtaacactat gctgatcggc tttctgcacg atctcagata
     1501 aaagcccggc tttatcgtta tgcccagaca ttgcatacat cagtgaagaa atgacttctg
     1561 gatactgtcc cagccaatca aacgcagatt ttggaataag ttgatttgca aggaagcctc
     1621 ctagcaccgg atgaagatgg tagtctcgac ctttgatgta tttgaattta taaggttgag
     1681 gtggtaaccc attccaagca aaccaacgtg aaccgtcttt gagtgtaatt tcaatatcaa
     1741 cgacaacttt gccaatatcg tgaac
//
LOCUS       DANLXS010000285         1723 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.293, whole genome shotgun sequence.
ACCESSION   DANLXS010000285 DANLXS010000000
VERSION     DANLXS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.293"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..668
                     /locus_tag="PXD71_002618"
     CDS             <1..668
                     /locus_tag="PXD71_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292675.1"
                     /translation="NPLIYHRHSWHLEFDGSVLYIADPTLFKYESINLAWYVGDKDTD
                     LYPYLAEFVTSVATHLNVETDHIVTYGSSGGGFAALQLGARIGNGVVTVCINPQTNIM
                     KYVPSHRNAFLNACFDFNAEEYDSLSNQEKFNALVLLKENTSKILYIQNTQDEFHHKW
                     HFLPFLELKGIDLSEIEKTHWREQQSKDVKVILYSHESGHAAEPKEMIPEILSAVDQL
                     IAS"
     gene            670..1665
                     /locus_tag="PXD71_002619"
     CDS             670..1665
                     /locus_tag="PXD71_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6292676.1"
                     /translation="MLFYLVSDSLTRLSLSNESLVDLGRITSIGNYPFLHNAKNAKFL
                     MVESAWQGYKNRWKYKIASYPDNPERTNEKLVRLVEKAKDKGIPTVFWNKEDSVHFDR
                     FIDSAKHFDHIFTVDQNAIARYREVVPATTTVNVGMFPVQPKIHNFQGFNFKHLAANF
                     VGSYSHHIHNKRRERQDMLFGAALKAGLPVTIFDRNSDRKSENYRYPKNSFNLNVQPN
                     VSYTETANIYRDYLVSLNINTVEDSPTMFSRRVVEILACGGILVSTPSMAIDNIFKEY
                     CHIVQSEDEAIELFQRLKRGASRDDLNKAIAGSEFVLSNFTWEHFLSQIQKVVYR"
BASE COUNT          565 a          278 c          316 g          564 t
ORIGIN      
        1 gaaatccctt aatttatcat agacatagtt ggcatctaga atttgatggc tcagttctat
       61 atatcgcaga tccaacatta tttaaatatg aatcaattaa tttggcttgg tatgttggag
      121 ataaagatac tgatttatat ccatacttag ctgaatttgt aacttctgtt gctacacatt
      181 tgaatgttga gacagaccat attgttactt atggttcaag tggtggtgga tttgctgcat
      241 tacagttggg agcaagaatt ggaaacggtg tagttaccgt ttgtattaat ccacaaacca
      301 acatcatgaa atatgtacca agtcatcgta atgccttttt aaacgcttgc tttgacttta
      361 atgcggaaga gtacgatagt ctttctaatc aagagaaatt taatgcttta gtattactta
      421 aggaaaatac atcaaagatt ttgtatattc aaaatactca ggatgaattt catcataaat
      481 ggcatttcct cccattccta gagttaaagg ggatagatct atcggaaata gaaaagaccc
      541 attggagaga acaacagagc aaagatgtaa aagttatttt gtattctcat gaatcaggtc
      601 atgcagctga acctaaggaa atgattcccg agatattatc cgctgttgat caactgatag
      661 ctagctaaaa tgctgtttta tttggtatct gattctttaa ctaggctttc attatcaaat
      721 gaaagcctag ttgatcttgg aagaataaca tctataggaa attatccttt cttgcataat
      781 gcaaagaatg ctaaatttct tatggtcgaa tccgcctggc aaggctacaa aaaccgttgg
      841 aaatataaaa ttgcctctta tcctgataat cctgaacgaa ccaatgaaaa gttggttcgt
      901 ttagtagaaa aagcgaagga taaaggcatt ccaacggtct tttggaacaa agaagattct
      961 gtgcattttg atcgatttat tgattcggcg aagcattttg atcatatttt taccgtagat
     1021 caaaatgcca ttgctcgtta tagggaagta gtgcctgcga caacaaccgt caatgtcgga
     1081 atgttccctg ttcagcccaa aatccataac tttcaaggat ttaattttaa acaccttgcc
     1141 gcaaattttg tagggagtta cagtcatcat atccacaata agcgtagaga aagacaggat
     1201 atgttgtttg gtgcagcact taaagcaggt cttccggtaa ccatctttga tagaaactct
     1261 gatagaaaat cggaaaatta tcgttaccca aaaaatagct ttaatttaaa tgttcaacct
     1321 aatgttagtt atactgaaac tgctaatatt tatcgtgatt atcttgtttc tttgaatatt
     1381 aatactgtcg aagattcccc tacgatgttt tctcgcagag tcgttgaaat attagcttgt
     1441 ggaggaatat tagtctctac accaagtatg gctatcgaca atatatttaa agaatattgt
     1501 catatcgtac aaagtgaaga tgaggctata gaattattcc aacgtttaaa acgaggtgct
     1561 tctcgtgatg atttgaataa agcgattgca ggctctgaat ttgtattgag taactttact
     1621 tgggaacatt tcttatccca aatacagaaa gttgtatata ggtaacttaa atagcattat
     1681 taaaaactct ctgccaagaa attattttct atttcgagta att
//
LOCUS       DANLXS010000286         1715 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.294, whole genome shotgun sequence.
ACCESSION   DANLXS010000286 DANLXS010000000
VERSION     DANLXS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.294"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            123..1619
                     /gene="pepA"
                     /locus_tag="PXD71_002620"
     CDS             123..1619
                     /gene="pepA"
                     /locus_tag="PXD71_002620"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDL6292677.1"
                     /translation="MEFSVKNGSVEKQRTACLVVGVYEPRRLCSAAEQLDKLSNGYIS
                     TLLRRGDLEGKAGQTLLLHNVPNVLADRVLLVGCGKERELTERQYKQIIQKSVQTINE
                     TGSMEAVSFLTELHVKGRTPYWNVRFAVEAIQEQLYAYNDFKSIKPEVRRELRRVIFN
                     VAHRKDLPDAERALEHGKAVALGISHAKNVANCPPNVCNPHYLAELAKGLAYDYPAIS
                     TTIVDEKQMAELGMNAYLAVSRASENEAFLSVIEYKGNPNADAKPIILVGKGMTFDSG
                     GLSIKPAEAMDEMKYDMCGAATVYGTIKAIAEMKLPLNVIGLLAGCENAVDGKAYRPG
                     DILTTMSGLTVEVLNTDAEGRLVLCDALSYVERFEPELVIDIATLTGACMIALGNHNS
                     GLMSPHNGLANELLAAAEQADDKAWRLPLGEEYQEQLKSNFADLANIGGRLGGACTAG
                     QFLSNFTKKYTWAHLDIAGTAWKSGAAKGATGRPVSLLTQFLINKAQV"
BASE COUNT          528 a          339 c          377 g          471 t
ORIGIN      
        1 tgcaaataaa aagtaaatta ttttaaataa tgatttacat cagggtatat ctatatttta
       61 cttatcattg atgattatgt tggcattata aaatctgtag caataaaaat taaggaggaa
      121 aaatggaatt tagtgtcaaa aatggcagtg ttgagaaaca aagaaccgcc tgtttggttg
      181 ttggtgtcta tgaaccccgc cgattatgtt ctgccgcaga acagcttgat aaattaagta
      241 acggctatat cagcacatta ctgagaagag gagatttaga aggcaaagca gggcaaacct
      301 tactcttgca taatgtgccg aatgtactcg ctgatcgagt attattagtc ggctgtggaa
      361 aagagcgtga attaactgaa cgccaataca aacaaattat tcaaaaaagt gtacaaacta
      421 ttaatgaaac cggttcaatg gaagctgtca gctttttaac cgaactgcac gttaaaggta
      481 gaacacctta ttggaatgtg cgatttgcgg tagaggcaat ccaagaacaa ctatatgcct
      541 ataacgattt caaaagcatc aaacctgaag ttcgccgtga attacgccgt gtgattttta
      601 atgtcgcaca ccgtaaagat cttcctgatg ccgaacgagc tttagaacac ggaaaagccg
      661 tagcacttgg aatcagccac gctaaaaatg tagcaaattg tccgcctaat gtgtgtaatc
      721 ctcactattt agccgaattg gctaagggtt tagcctacga ctatcctgcc atttcaacta
      781 ccattgttga tgaaaaacag atggcagaat tgggaatgaa tgcttactta gcggtttcca
      841 gagcctctga aaatgaggcc tttctttcag tgattgaata taaaggcaac ccgaatgctg
      901 atgcgaagcc gattatctta gtcggcaaag gaatgacctt tgactctggc ggtttatcca
      961 ttaaacctgc ggaggcgatg gacgaaatga aatacgatat gtgcggagct gctactgttt
     1021 acggtacaat caaagcaatt gccgagatga aattaccact caatgttatc ggcttactcg
     1081 ccggctgtga aaatgcggtt gatggcaaag cctatcgacc gggggatatt ctcactacaa
     1141 tgtctggttt aaccgttgag gtactcaaca ccgatgcaga aggacgttta gtgctatgtg
     1201 atgctctcag ctatgtagaa cgttttgaac cggaattagt gattgatatt gctaccctaa
     1261 ctggtgcttg catgattgcc ttaggtaatc ataacagcgg gctgatgtca cctcataacg
     1321 gtttagcaaa cgaactctta gctgccgcag agcaagccga tgacaaagcg tggcgtttac
     1381 ctttagggga agaatatcaa gaacagttaa aatctaactt tgctgatctt gctaatatcg
     1441 gcggacgttt aggcggagcc tgtactgccg gtcaattcct ctctaacttt acaaagaaat
     1501 atacttgggc gcatctagat attgccggca cggcttggaa atctggggca gccaaagggg
     1561 cgaccggcag accggtatcc ttactaacac aatttttgat caataaagcc caggtctaac
     1621 tctcacaagc ggtctgattt tatcgagttt ttgcaaaaag aaaatcagac cgatcctctt
     1681 catacgcccc tatattcttt ttagttatat caaat
//
LOCUS       DANLXS010000287         1708 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.295, whole genome shotgun sequence.
ACCESSION   DANLXS010000287 DANLXS010000000
VERSION     DANLXS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.295"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            109..489
                     /locus_tag="PXD71_002621"
     CDS             109..489
                     /locus_tag="PXD71_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292678.1"
                     /translation="MKFIDFGKQLAKPVKFIKQRTAFLPKPLQQAVVFAVIFAGLPLF
                     LVGFTVLRILNGTPVEDISTNDVAYNPNHVENTVNEIEDDSEDSKPEDLWDVIADQDE
                     NQLDGYRDGYFGYGLYQGGYFVKR"
     gene            complement(542..>1708)
                     /locus_tag="PXD71_002622"
     CDS             complement(542..>1708)
                     /locus_tag="PXD71_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL6292679.1"
                     /translation="QMNSELGNQQARVPLWWGLFHQINKGVTHALTASIPCKPDLRQV
                     RFEVQHEKINNPALLTEVRLFVQQCYVPARQKIQSSQLSLTPAQVRETSWLGGRILLT
                     NSELYPRYRAQQPHSLFPYNASRDNGLPNNGEGGFPNCAEWWDTANTGLKDRLLADVK
                     QNFSAKVIDFFSSKENADESILRTVLRAENMDISRGKVYPGYGGNLDPTFANATTRLT
                     SSVGSLAASFAAFPAFDAMRQALPMVHAFMLMAVIIITPLVIVMSGYSLKTLVTLTFV
                     SFALVSLTFWWELARWLDSWLLSALYDSSAHSRINPYFLENTQDDFIVNFVMGSMFLV
                     LPAIWLGSMSWAGVKIGDIAAGFTTDSLDSKSAGSQGGNLVKKGGKEISAGFNK"
BASE COUNT          546 a          348 c          321 g          493 t
ORIGIN      
        1 agataattta acaggaaaag ttttatctta ttctgtaaaa gacaataaag ataaagtaat
       61 ttatagtagt cgccaaaaac aataaccttt tatcttagga aagatactat gaaatttatc
      121 gatttcggaa agcagctcgc taagccggtt aaatttatca aacaacgtac cgcattttta
      181 ccaaaaccgc tacaacaagc tgtcgttttt gcggtgattt ttgcaggact tcccttgttt
      241 ttagttggat tcacggttct gcgaattctc aatggaacac cagtagaaga tatttcaaca
      301 aatgacgtag catataatcc aaaccacgtc gaaaacacgg ttaatgaaat tgaagatgat
      361 agtgaggata gtaagccgga agatttatgg gatgtaattg ctgatcaaga cgaaaatcag
      421 cttgatggat atcgtgatgg atattttgga tatggactct atcagggtgg ctattttgta
      481 aaaagatgag aaaatatgat tagaaattaa taaggtagag attaacctct accttattgt
      541 tttatttgtt aaaccctgca gatatttcct taccgccttt cttaactaaa ttaccacctt
      601 gagagcctgc ggatttacta tctaatgaat cagtagtaaa accggcagct atatctccta
      661 tttttactcc agcccacgac atactgccta gccaaatcgc cggtaaaacc aagaacatcg
      721 aacccatcac aaaattgacg ataaaatcat cttgcgtatt ttcaaggaaa tacggattta
      781 ttcgactatg tgcactggaa tcatatagag cggatagtag ccaagaatct aaccatcttg
      841 ctaattccca ccagaatgtc agtgatacca aagcaaaact cacaaaagtt aatgtaacta
      901 atgttttgag agaataacca ctcatcacaa taaccagcgg agtaatgatg atcacagcca
      961 tcaacataaa agcatgtacc atcggcagtg cttgtcgcat tgcatcgaat gctggaaagg
     1021 ctgcaaagct ggcagctaag cttcccactg aagatgttaa tcgagtcgtt gcatttgcaa
     1081 atgtaggatc taaattcccc ccatacccgg gataaacttt ccctctcgaa atatccatat
     1141 tctctgctcg taatacagta cggagtatgg attcatccgc attttctttg ctgctaaaaa
     1201 aatcaatcac ttttgcagag aagttctgtt tgacatcagc taataaacga tcttttaaac
     1261 cagtattcgc cgtatcccac cactctgcac aatttggaaa accaccttcc ccattattcg
     1321 gcaatccatt atcccgggaa gcattgtagg gaaatagact atgaggttgt tgagctcgat
     1381 aacggggata aagctcactg tttgtcaaaa gaatacgacc acctaaccag ctggtttcac
     1441 gcacctgtgc cggagtcaat gagagctgac tgctttgaat tttctgacgt gcaggtacat
     1501 aacattgttg tacaaataag cgaacctcag tcaataatgc aggattattg attttttcat
     1561 gttggacctc aaagcgaact tgacgtaaat ccggtttaca cggaattgaa gctgttaatg
     1621 cgtgtgtaac tcctttgttg atttgatgga ataatcccca ccaaagcggt actctcgctt
     1681 gctggttgcc caattcagag ttcatttg
//
LOCUS       DANLXS010000288         1708 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.296, whole genome shotgun sequence.
ACCESSION   DANLXS010000288 DANLXS010000000
VERSION     DANLXS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.296"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..526
                     /locus_tag="PXD71_002623"
     CDS             <1..526
                     /locus_tag="PXD71_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL6292680.1"
                     /translation="DFLKPSWFILAIIMTLRCTGIRRGQLVKLKIGDVNLSNRTIYIS
                     PEINKNHDYHIVPISDSLYLHIERLITELKIRKQTDNCQLFNLNLFSRCSRRRGQPMS
                     QNQLTHIFRHISDLVGFTSSPHRFRHTVATQLMKKPENVYVVQKLLGHKDISVTLGYI
                     EHDVEMLRETVNLL"
     gene            707..1678
                     /locus_tag="PXD71_002624"
     CDS             707..1678
                     /locus_tag="PXD71_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISApl1 family transposase"
                     /protein_id="HDL6292681.1"
                     /translation="MSTSYRHLTINEREKIMILLAQGKKQAEIAKALGRSSSTISREL
                     KRHALESYSATNAQNSYLKHRQNSKAQRKLEQPEYFNLVQEKFLTENWSPEQISARLK
                     LEKSELSISYSTIYRGIYSGLFDIGERKASRKLRHKGKTRHTKNHHEKRGKIQISNHL
                     NDRPISAQNRSRFGHWKADTVLGKAGGACLLTLTERKSRFELVKKIPAKKAEAVQKAM
                     IELLDSHILRSITPDRGKEFAQHRLVTEALGVEFYFPEPHQPWTRGTNENTNGLLREY
                     FPKHQDINQWSEVDIQQVVNKLNLRPRKCLGWKTPYEVYFKKTLHLV"
BASE COUNT          596 a          329 c          328 g          455 t
ORIGIN      
        1 tgattttcta aaaccaagtt ggtttattct tgcgattatt atgacattga gatgtacagg
       61 cattcggcga ggacaattag tgaaactgaa aattggtgat gtaaatcttt ccaacaggac
      121 gatttatatt tcgccagaaa tcaataaaaa tcatgattac catatcgtac cgatttcaga
      181 ttcactttat cttcatatag aaaggcttat tacagagtta aaaatcagaa aacaaacaga
      241 taattgccaa ttgttcaatc tcaatttatt ttctcgatgc tctcgaagaa gaggacagcc
      301 aatgagccaa aatcagctta cgcatatatt tcggcatatt tctgacttgg tcggttttac
      361 ttcatcacca catagatttc gacatacggt tgcaactcaa ttaatgaaga agcccgaaaa
      421 tgtttatgtg gttcagaaac ttctagggca taaggatatc agcgtaacac taggttatat
      481 tgagcacgat gtggaaatgt tgagagagac agtgaattta ctctgataaa agaaaaatgg
      541 gagaatataa tttctcccac tttttttcta taaataaata atttctgttt tatcttctaa
      601 atgaactgat tttatacgct gaatttacaa tccaagtgca acaaaaaaag aagtactcat
      661 caactagaat ataattttgt ttccacacaa aaaccacttc caaatgatga gtacttccta
      721 ccgacatctt acaataaacg agcgagaaaa gataatgatt ttactcgcac agggcaaaaa
      781 acaagcagaa attgccaaag cactgggacg tagctccagc accatttctc gcgagctgaa
      841 acgacacgct ctagaaagct acagtgcaac gaacgcacaa aacagctatt tgaagcatcg
      901 tcaaaatagc aaagcacagc gcaaattaga gcagcctgaa tatttcaatt tggtgcaaga
      961 aaagtttctg acagaaaact ggtcgcccga acaaatcagc gcacgattaa aattggaaaa
     1021 atctgaatta tccattagtt attcaaccat ttatcgtggt atttattcag ggttgtttga
     1081 tataggcgaa cgcaaagcca gtcgcaaact gcgccacaaa ggcaaaacac ggcatacaaa
     1141 aaatcatcat gaaaaacgtg gcaaaattca gatatccaac catttgaacg accgtcccat
     1201 ttcggcgcaa aatcgcagtc gctttggaca ttggaaagcc gataccgtac tgggtaaagc
     1261 gggtggagct tgtttgctga cgctgacgga acgcaaaagt cgttttgagt tggtgaagaa
     1321 aattcctgcc aaaaaagccg aagcagtcca aaaagccatg attgaattgc tggattcaca
     1381 tatattgcgg tcaattacgc cagaccgtgg taaagaattt gcccaacatc gtttggtaac
     1441 agaagcactg ggtgtagaat tttacttccc cgagccgcat caaccgtgga cacggggaac
     1501 gaatgaaaat acaaatgggt tacttcgtga atactttccg aagcaccaag acatcaatca
     1561 gtggagcgaa gttgatattc aacaggtggt caataaactg aatttacgac cgcgtaaatg
     1621 tttgggttgg aaaacacctt atgaagttta cttcaaaaaa acgttgcact tggtttgaca
     1681 attcaagctt ccaaattaaa atttttaa
//
LOCUS       DANLXS010000289         1689 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.297, whole genome shotgun sequence.
ACCESSION   DANLXS010000289 DANLXS010000000
VERSION     DANLXS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.297"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            24..>1689
                     /locus_tag="PXD71_002625"
     CDS             24..>1689
                     /locus_tag="PXD71_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL6292682.1"
                     /translation="MLSKLQRFTLMIKSTIIKHPLEILFVTYVTILLINNVSDYELIK
                     RFQNLIALFPICFILLYLIRDTKAYWLFAPIPIAIVFFFGQINLTLLEDSRYWATIFC
                     VFLCLISQYWYKNNHYFVAGTTNKLVNIVFATVLSGIIFATLSAITIAIIALFDLNKQ
                     NNFHIFEKFGIFSSFWAFPVLFLTLEQQTTNDSRYLNRISEMLLNWILSPALIIYTAI
                     IYAYVIFIAAKGQMPDGVVANVALPYLAFGLAIQAVQLLLKNAKWQWFYSHFTYLALL
                     PLTLIWYAIYIRFSHYGLTEARIYLVIGTITLSICYTLLLFKQLAQYRLFSAVSIAII
                     LISVFILDPKTVALKDQTKRLDNYLLKHKLLDVNNKISTKAVLKYQESLNENRDEAEY
                     FYSMLHYVARGMPAAQFKEKYGVNSSYDLMSEIRERNTDPRYFEATNSELVRLTKADM
                     ANAREYITFSSQLYRPNSFSHQKIENKLCQKLLSQGYYFEGNILNIEYQYSETAQDCS
                     SITQDPTEFVIYFKGLGTTIEFNINDILKKIFAKHQLSITQQHKNEV"
BASE COUNT          542 a          300 c          254 g          593 t
ORIGIN      
        1 ttttcatagg caggagatct attatgctaa gtaagctaca acgctttacc ttaatgataa
       61 aaagcaccat cattaagcat ccactggaaa ttttattcgt tacttacgtc acgattttac
      121 ttatcaataa tgtgagcgac tacgaattaa tcaaacgctt tcaaaatcta attgctcttt
      181 ttcctatttg ctttattttg ctttacctga tacgcgatac caaagcttat tggctattcg
      241 ctccgattcc gatagcaatc gtctttttct ttggtcaaat taatttaaca ttattggaag
      301 attctcgtta ttgggctacc atattttgtg tattcctttg tttgatcagt caatattggt
      361 acaaaaacaa ccattatttt gtagccggaa caactaataa attagttaac attgtctttg
      421 ctacagtttt atctggcatt atttttgcaa cattaagcgc tattaccata gccattattg
      481 ctttatttga tttaaataaa caaaacaact ttcatatttt tgaaaaattt ggcatttttt
      541 ctagtttttg ggcattccct gttttatttt taactcttga acaacagact accaatgatt
      601 ctagatatct aaaccgcatt agtgaaatgt tactaaactg gattttgtct ccggcattaa
      661 ttatctatac tgcaattatt tatgcttatg taatttttat tgcggctaag gggcaaatgc
      721 cagatggggt tgttgcaaac gtagcgcttc cctatttagc tttcggccta gctatacaag
      781 cggtgcaatt attactaaaa aatgcaaaat ggcagtggtt ttattctcac tttacctatt
      841 tagcactttt gccgttaaca ttaatttggt atgccatcta tattcgtttc agccattatg
      901 gcttaactga agcccgtatt tatttagtga ttggcacaat aaccttatct atttgctata
      961 ccttattact atttaaacag ttggcgcaat atcgtctctt ttctgcagtg tcgatagcaa
     1021 ttattttaat ttcagtgttt attttagatc cgaaaacagt tgcgcttaaa gatcaaacca
     1081 agcggttgga caactatttg ctaaaacaca aattattaga tgtaaataac aaaatcagca
     1141 caaaagctgt acttaaatac caagaatcat taaatgaaaa ccgagatgaa gccgaatatt
     1201 tttattcaat gctccactat gttgccagag gaatgcctgc tgcacaattt aaagagaaat
     1261 atggcgtaaa ttcaagctac gatttaatga gtgaaataag agaaagaaat actgacccac
     1321 gttattttga agctactaat tctgagctgg ttcgcttaac taaagcagat atggcaaatg
     1381 ctcgtgaata tattacattt agtagccaat tatatcggcc taattcattt tcccatcaaa
     1441 aaatagaaaa taagctatgc caaaaactcc tttctcaagg ttactatttt gagggaaata
     1501 tattaaatat tgagtatcaa tactcagaaa cggctcaaga ctgctcttca ataacacaag
     1561 atcctactga atttgttatc tactttaaag gacttgggac tactattgaa tttaacatta
     1621 acgatatttt gaaaaaaatc tttgctaaac atcagttatc tattacccag caacacaaaa
     1681 atgaggttt
//
LOCUS       DANLXS010000290         1680 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.298, whole genome shotgun sequence.
ACCESSION   DANLXS010000290 DANLXS010000000
VERSION     DANLXS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.298"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            112..768
                     /locus_tag="PXD71_002626"
     CDS             112..768
                     /locus_tag="PXD71_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6292683.1"
                     /translation="MNTQTLGDRIKSRRKELKLTQKDLATALKGVSHVAVSQWESSTT
                     KPNSENILDLSTVLQCDISWLLRGNGESNVLPASLGMTKVPLISYIQAGQWTGIDDFR
                     ETCGDYEYILTDLEVSEDAFALEIKGDSMEPDFIEGDRVIIDPDVQPHAGEFVAAING
                     DYEATFKKYRPLEDLDEYGRQHFELIALNPDYHKLSTLKQEIRIIGTMVEHRIYRRKR
                     "
     gene            780..1154
                     /locus_tag="PXD71_002627"
     CDS             780..1154
                     /locus_tag="PXD71_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292684.1"
                     /translation="MTEFKDVKQAIVEAQLASTPKDKVVNDIMKGSAELLSANDIINR
                     LGIGADEFQELVNLPNHYLRINGGLGAAVYNTRRMFDTHVELVNQELEKNISFPKPDL
                     YILGKARWKKETFKKWLEEQCR"
     gene            1306..>1680
                     /locus_tag="PXD71_002628"
     CDS             1306..>1680
                     /locus_tag="PXD71_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL6292685.1"
                     /translation="MLSKQVLSISESRLSTYLFCFFRNDPTKQKEAIAIYTALQHRTG
                     IYFSLIQEIEVALRNEVSQLLRFAAPNHNLYEFFHFLANDSNAPLNAESKRQLQKAIS
                     ECGRNYNENDIISHISFGFWVNL"
BASE COUNT          573 a          270 c          339 g          498 t
ORIGIN      
        1 ctttcttgaa ttttatttaa gacctcttaa atatacaatt aaaggaaact taagtcaata
       61 aatgttaaga taacttaaca gcaactttat ttttaccttt aaggggatac tatgaatact
      121 cagaccttgg gcgatagaat taaaagccgt agaaaagaat taaaattgac acagaaagat
      181 cttgctactg cgttaaaagg ggtctcacac gttgcagtat ctcaatggga aagtagtacg
      241 actaagccaa attcagagaa tattttggat ttatctacag tattgcaatg cgatattagt
      301 tggctcttgc gtggtaatgg agagtctaat gttctacctg ctagcctcgg tatgactaaa
      361 gtcccattaa ttagctatat tcaagccgga caatggacgg gaattgacga ttttcgtgaa
      421 acctgtgggg attatgaata tatcctaact gatcttgagg tgtctgaaga tgcttttgcc
      481 ctagaaatca aaggggactc gatggagcct gattttattg aaggagaccg agtaattatt
      541 gatcctgatg tacaacctca cgccggtgag tttgtggcag caattaatgg cgattatgaa
      601 gccactttca aaaaataccg tccgttagaa gacttagacg aatacggaag acaacatttt
      661 gagcttatcg ctcttaatcc ggattaccat aaattatcaa cgctaaagca agaaatcaga
      721 attatcggca caatggtaga gcatcgtatt tatcgtagaa agagatagga attgataata
      781 tgacagaatt taaagatgta aaacaggcga ttgtagaagc tcaattagct tcgacaccta
      841 aagataaagt cgttaatgac ataatgaaag gaagtgcgga actgctttca gcaaatgata
      901 ttatcaatag gttaggtatt ggggcagatg agtttcaaga gttggttaat ttacctaacc
      961 attatttgcg tataaatgga gggttaggtg ctgctgtgta taatacaaga agaatgtttg
     1021 atacccatgt tgagttagtc aatcaagagt tagaaaagaa tatttcattt cctaaacctg
     1081 atttatatat tttggggaaa gctcgttgga aaaaagaaac atttaagaaa tggttggaag
     1141 aacagtgtag ataaagaaaa ggtaggttaa aaaacctacc ttttctgaac cgcaaataag
     1201 cgattctcac actttacaaa gcaatccagc tcctacgtct ggggcttcgg gtccgttgcc
     1261 agtgcaagta ttattacatt aaactaaact tgagtaaagg aaaatatgtt gtccaaacaa
     1321 gtgctaagta tttcagaaag tcggctgagt acatatttat tttgcttttt ccgaaatgat
     1381 cccacaaaac agaaagaagc tattgcaatt tataccgcct tgcaacatcg aacaggaatt
     1441 tatttttcat tgattcaaga aatagaggtg gcactaagaa atgaagtcag ccagttgtta
     1501 cgctttgctg cacctaatca taatttatac gaattctttc attttctagc gaacgatagt
     1561 aatgctcctc ttaatgcaga atctaaacgc cagctacaaa aagccattag tgaatgcgga
     1621 cgtaactata atgaaaatga cataatttct catatttctt ttggattttg ggtaaatctg
//
LOCUS       DANLXS010000291         1680 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.299, whole genome shotgun sequence.
ACCESSION   DANLXS010000291 DANLXS010000000
VERSION     DANLXS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.299"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..140
                     /locus_tag="PXD71_002629"
     CDS             <1..140
                     /locus_tag="PXD71_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDL6292686.1"
                     /translation="QTFINASNLDEKQMMWQVVIHLAFIVSALAMAYIDKMLNSGHMK
                     H"
     gene            217..1587
                     /gene="gorA"
                     /locus_tag="PXD71_002630"
     CDS             217..1587
                     /gene="gorA"
                     /locus_tag="PXD71_002630"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL6292687.1"
                     /translation="MAKHYDYIAIGGGSGGIASINRAASYGQKCAIIEAKHLGGTCVN
                     VGCVPKKVMFYGAQVAEAINHYAPDYGFDITVNNFDYAKLVESRQAYIGRIHTSYGNV
                     LAKNNVDVLNGFARFKDAKTLEVSYADGSVEEVTADHILIATGGRPTVPNVKGAEYGI
                     TSDGVFALDALPKSVAIVGAGYIAVELAGVFNSLGVNTHLFVRQHAPMRNQDPLIVDT
                     LLEVLAQDGIELHTQATPQEVVKNADGSLTLKLQDGRETSVESLVWAIGREPATDKIN
                     LEAAGVETNERGFIKVDKFQNTNVPNIYAVGDIIEGGIELTPVAVAAGRRLSERLFNN
                     KPNEHLDYSLVPTVVFSHPPIGTVGLSEPKAIEQYGAENVKVYKSSFTAMYTAVTQHR
                     QPCRMKLVCVGEGEKVVGLHGIGFGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSE
                     EFVTMR"
BASE COUNT          501 a          254 c          388 g          537 t
ORIGIN      
        1 tacaaacatt tatcaatgct tcaaatcttg atgaaaagca aatgatgtgg caagtagtta
       61 ttcacttagc ctttattgtt tcagcattag cgatggcgta tattgataaa atgttgaatt
      121 cagggcatat gaaacattag tcttctatgc aaatttaatt tgataaagcg cttaacgaat
      181 aagttgcttt tgtttttata gatcgaggaa tagcttatgg ctaaacatta tgattatatt
      241 gctatcggcg gtggtagtgg tggtattgct tcaattaatc gagcagcaag ctatgggcaa
      301 aaatgtgcca ttattgaagc aaaacattta ggtggcactt gtgtgaacgt gggatgtgta
      361 cccaaaaaag tgatgttcta tggtgcacaa gttgcagaag cgattaacca ttacgcccct
      421 gattatggtt ttgacattac ggtaaataat tttgattatg ccaaattagt tgagagtcgc
      481 caggcctata ttgggcgcat tcatacctca tatggtaatg tgctggcaaa aaataatgtt
      541 gatgttttaa atggttttgc tcgctttaaa gatgctaaaa cattagaagt gagttatgct
      601 gatggttccg ttgaggaggt cacagcagac catattctga ttgcaacagg tggtcgtcca
      661 actgtaccca atgtgaaagg ggctgaatac ggtatcactt ctgatggtgt atttgcacta
      721 gatgctttac cgaagtccgt agcaattgtg ggggcagggt atattgcagt tgaattagct
      781 ggagtattta atagtttagg agtaaatacc catctttttg ttcgtcaaca tgctccaatg
      841 cgtaatcaag acccattaat agtggataca ttgcttgaag tattagctca agatgggatc
      901 gaattacata cacaagcgac tcctcaagaa gtagttaaaa atgcagatgg ttcattgaca
      961 ttaaaattgc aagatggtcg tgaaacctct gttgaaagct tggtgtgggc tattggtcgt
     1021 gagccagcga cagataagat taatttagag gctgctggtg ttgaaaccaa cgagcgtggt
     1081 tttatcaaag tggataaatt ccaaaatact aatgtgccaa atatctatgc tgttggtgat
     1141 attattgaag gtggtattga attaactcct gttgcagttg cagctggtcg caggctatcg
     1201 gaacggttgt tcaataataa accaaatgaa catttggatt atagcttagt accgacagtc
     1261 gtttttagcc acccaccaat aggtactgtt ggtttaagtg agccaaaagc gattgagcaa
     1321 tatggggcag aaaatgtgaa agtgtataaa tcttcattta ctgcaatgta tactgcggtt
     1381 acacaacatc gtcaaccttg ccgaatgaaa ttagtttgtg ttggggaagg ggaaaaagtg
     1441 gttgggttac atggtattgg ttttggtgtt gatgaaatga ttcaaggttt tgctgttgca
     1501 attaaaatgg gcgcaacgaa agctgatttt gacaacacag tggctattca cccgacagga
     1561 tctgaagaat ttgtaaccat gcgctaaatg taataaaatg ttacgtaatt ttatgattat
     1621 gctacatttt atttatgggt ttgctatgaa aaacgctttt agcttttttg ttatttctct
//
LOCUS       DANLXS010000292         1578 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.300, whole genome shotgun sequence.
ACCESSION   DANLXS010000292 DANLXS010000000
VERSION     DANLXS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.300"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            70..411
                     /locus_tag="PXD71_002631"
     CDS             70..411
                     /locus_tag="PXD71_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292688.1"
                     /translation="MKFIDFGKQLANPVKFIKQNTAFLPKPIQQVVVFAVIFAGLPLF
                     ILGFFILRIWAYKHISLYSEYKDMANSEISSKDTDLKSLFSDDEEQEDDEYQYGYDGP
                     GIYLGNYRVDE"
     gene            complement(457..>1578)
                     /locus_tag="PXD71_002632"
     CDS             complement(457..>1578)
                     /locus_tag="PXD71_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL6292689.1"
                     /translation="QMNSELGDQQARVPIWWGLFHQINKGVTHALTASIPCKPDLRQV
                     RFEVQHEKINNPALLTEVRLFVQQCYVPARQKIQNSQLSLSPAQVRETSWLGGRILIA
                     NSELYPRYRAQQPHSLFPYNASRDNGLPNNGEGGFPNCAEWWDTANNGLKDRLLDDVK
                     QNFSAKVIDFFSSKENSDESILRTVLRAENMDISRGKVYPGYGGNLDRSFTNEVTKTA
                     SMFGLSLGSIGAFPAFDSMRQALPMVHSFALMALIILTPLIMVMSGYSLKTLVTLTFV
                     NFALVTLTFWWELARWLDSWLLSVLYDSGTHSRINPYFLENTQDDIIVNFVMGSMFLV
                     LPAIWLGSMSWVGVKMGEFASHLTTGSRDSKTAGSKIGK"
BASE COUNT          499 a          311 c          282 g          486 t
ORIGIN      
        1 tgaatataca actttaatta attatcatac tgagctgaaa atcagctaat tagaataatt
       61 ggaggagcta tgaaatttat cgatttcgga aagcagctcg ctaacccagt taaatttatc
      121 aaacagaata ctgcattttt acctaagcct attcaacaag tggtcgtttt tgcggtaatt
      181 tttgcaggat tgcctctatt tatcttgggt ttctttattc tcagaatatg ggcttataag
      241 catatttctt tgtatagtga atataaagat atggctaatt cagaaatatc gtctaaagat
      301 acagatttaa aatcactttt ttctgatgat gaagaacaag aagatgatga atatcaatat
      361 gggtatgatg gaccaggtat atatctaggt aattaccgtg ttgatgagta aaagtaaata
      421 gagtggtata taccactcta ttattcagtt gttagattat ttccctatct tggaacccgc
      481 tgttttagag tctctagaac cagtagttaa atgacttgca aactctccca tttttactcc
      541 tacccaagac atactgccta gccaaatcgc tggtaagacc aagaacatag aacccatcac
      601 aaaattcaca ataatatcgt cttgtgtatt ttcaaggaaa taagggttta ttctgctatg
      661 tgtgcctgaa tcatagagga ccgacagcaa ccaagagtca agccatctag ctagttccca
      721 ccaaaatgtg agtgtaacta atgcgaaatt cacaaaagtt agcgtaacca atgtttttaa
      781 cgaataacca ctcataacca taattaaagg cgtgagaata atcaatgcca ttaatgcaaa
      841 tgaatgcacc atcggtaatg cttgccgcat cgaatcaaaa gcagggaatg ctccgatact
      901 gcctaatgac agaccaaaca ttgaagctgt tttagtcact tcatttgtaa atgatctgtc
      961 taaatttccc ccatacccgg gataaacttt ccctctcgaa atatccatat tctcagctcg
     1021 taatacggta cgaagtatgg attcatcgga attttcttta ctgctgaaaa aatcaatcac
     1081 ttttgcagag aagttctgtt ttacatcatc taataaacga tcttttaaac cattattcgc
     1141 cgtatcccac cactctgcac aatttggaaa accaccctct ccattattcg gcaatccatt
     1201 atcccgagaa gcattatagg gaaatagact atgaggttgt tgggctcgat aacggggata
     1261 aagctcgcta tttgcaatca aaatacggcc acctaaccag ctagtctcac gaacctgtgc
     1321 tgggctcaat gaaagttggc tattttgaat tttctgacgt gccggtacat agcattgttg
     1381 tacaaataag cgaacctctg tcaataatgc tggattattg attttctcgt gttgaacttc
     1441 aaaacgaact tgacgtaaat ccggtttgca gggaattgaa gctgttaagg cgtgtgttac
     1501 ccctttgttg atttgatgaa ataatcccca ccagataggg actctcgctt gttgatcacc
     1561 caattcagag ttcatttg
//
LOCUS       DANLXS010000293         1574 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.301, whole genome shotgun sequence.
ACCESSION   DANLXS010000293 DANLXS010000000
VERSION     DANLXS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.301"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..161
                     /locus_tag="PXD71_002633"
     CDS             <1..161
                     /locus_tag="PXD71_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292690.1"
                     /translation="IMARIEKEQPLEKTLILYSEQLASFEQKSPMALHREKFEAKPTG
                     LAELINNL"
     gene            172..1122
                     /locus_tag="PXD71_002634"
     CDS             172..1122
                     /locus_tag="PXD71_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HDL6292691.1"
                     /translation="MSLSTFAHALKLDKQGNVILFDDSPEEQAKLISRLKQNRLFKLL
                     NLVQTKHAKGEAIGLFTPIAGTTNTESGEEREPKQALAPAQKYHCEQINIDSYIRYEK
                     IDAYNLENSLEERLNQLLDDNLLRGLLMVGWQGVSRADTSNATNNQLAQDVKKGWLQK
                     IREGKPEAVINGATIGKEQQYKSLNALIKQGLGKISPAYAMGGDMIAICGRDIIADDV
                     IQLEHNDLSENLADLLTLCQQAKGGLKAVSIPYFPSNAILITRLDNLSLYLHLNNIRR
                     KAFDTPQKDSVETFYSLNVDYIVEDFNACCLIENIEVIEE"
     gene            1119..1313
                     /locus_tag="PXD71_002635"
     CDS             1119..1313
                     /locus_tag="PXD71_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292692.1"
                     /translation="MSLESYIQQAEEMELLEKLDFVSDLLELEKVSEIEAYRILTAHG
                     EPKPQPAQEKLIGCVDFLDD"
BASE COUNT          583 a          319 c          299 g          373 t
ORIGIN      
        1 atataatggc tagaattgaa aaggaacagc cacttgaaaa aacattaatt ctttattctg
       61 aacagttagc aagttttgaa caaaaatcgc caatggcatt acaccgtgaa aaatttgaag
      121 caaaacctac cggcttagct gaattaatta ataacttata agaggtaaaa aatgagttta
      181 tccactttcg cccacgcatt aaaactagat aaacaaggta acgttatttt atttgacgat
      241 agccccgaag aacaggcaaa actaattagc cgcttaaaac aaaatcgcct ttttaaatta
      301 ttgaatttag tgcaaaccaa gcacgcaaag ggcgaagcga ttgggctatt cacaccgata
      361 gccggcacaa ccaacaccga aagcggagag gaacgagagc caaaacaagc ccttgcaccc
      421 gctcaaaaat accactgcga acaaatcaac attgatagct atattcgcta tgaaaaaatc
      481 gatgcttaca acctcgaaaa cagcttagaa gaacgcttaa atcagttact tgatgacaac
      541 cttctaagag ggcttttaat ggttggctgg caaggcgtaa gccgtgcaga tacatcaaac
      601 gcgacaaaca accaactcgc ccaagacgtg aaaaaaggct ggctacaaaa aatcagagag
      661 ggcaagcccg aagcggtaat taatggagca accataggca aagagcaaca atacaaaagc
      721 ctaaatgctc tgattaagca gggattaggc aaaattagcc cagcgtatgc aatgggcgga
      781 gatatgatcg ccatttgtgg gcgggatatt atcgctgatg atgtaattca gcttgaacac
      841 aacgatttaa gcgaaaacct agccgactta ttaaccttat gccaacaagc taaaggcgga
      901 ctaaaagcgg taagtattcc ctacttccca agcaatgcga ttttaatcac tcgattagat
      961 aacctttcgc tatatcttca tttaaacaat atccgccgta aagcgtttga tacgccacaa
     1021 aaagacagcg tagagacatt ttactccctc aatgtggatt acattgtaga agattttaac
     1081 gcctgttgtt taattgaaaa tattgaggtt attgaagaat gagcctagaa agctatatcc
     1141 aacaagcgga agaaatggag cttttagaaa aactcgattt tgtttcagac ctgttagaac
     1201 ttgagaaagt cagcgaaatt gaagcctatc gcattttaac cgcccacggc gaacctaaac
     1261 cccaaccggc acaagaaaag ctaataggtt gcgtagattt tcttgatgac taacccaagc
     1321 cccgaaaggg gctttttgca tttttgcaac aaaaaacgac cgcttgcacc ctttgaaatt
     1381 attgctatca ttaataaaaa acaataacca aatcaaatga gagtacagca atgacaaact
     1441 ataaactacc ggctagaaag tggtacacct tagagcaggc aacaaaacgc attaaacaac
     1501 tcactggcga agatatagaa atagaagatt tattacatta ttggtattta agaaagctag
     1561 aattaagtat ttat
//
LOCUS       DANLXS010000294         1508 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.302, whole genome shotgun sequence.
ACCESSION   DANLXS010000294 DANLXS010000000
VERSION     DANLXS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.302"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            65..1429
                     /gene="glmU"
                     /locus_tag="PXD71_002636"
     CDS             65..1429
                     /gene="glmU"
                     /locus_tag="PXD71_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL6292693.1"
                     /translation="MTQLSVVILAAGKGTRMYSDLPKVLHPVAGKPMVKHVIDTAKQL
                     DAQQIHLIYGHGADLLKARLSEEPVNWVFQAEQLGTGHAMQQAAPFFADDENIVMLYG
                     DAPLITKATLERLIAAKPENGIALLTVELENPTGYGRIIRENGSVVAIVEQKDANEAQ
                     LKVKEVNTGVMVASGASFKKWLKNLNNNNAQGEYYITDVIAMANQEGFKVQAVQATEF
                     MEVEGANNRLQLAALERFYQKREAEKLLLAGVTIIDPSRFDLRGTVTHGKDVQIDVNV
                     ILEGEIKLGNNVKIGAGCVLKNCEIGDNVEIKPYSVIEDSIVGAKSAIGPFSRLRPGA
                     ELAEETHIGNFVEIKKATIGKGSKVNHLTYVGDAEIGKECNIGAGVITCNYDGANKFK
                     TIIGDNVFVGSDSQLIAPVTIASGSTIGAGATVTKDIAENELVISRVPQRHIQGWQRP
                     TKKK"
BASE COUNT          496 a          304 c          330 g          378 t
ORIGIN      
        1 atcacttata gcaatttaca cgttactata agccttcatc tatttagtca aataaggatt
       61 ctatatgact caactgagcg ttgttatttt agctgccggt aaaggcactc gtatgtattc
      121 agatttaccc aaagtgttac acccagttgc cggtaaaccc atggtaaaac acgttattga
      181 tactgccaaa cagctagatg cccagcaaat tcatttaatt tacggacacg gtgccgatct
      241 gttaaaagca cgtttaagcg aagagcctgt aaactgggta ttccaagccg aacaattagg
      301 cacaggacat gcaatgcaac aagcagctcc ttttttcgct gatgatgaaa acattgtaat
      361 gctttatggt gatgcccccc taatcacaaa agccacttta gagcgtttaa tcgcagccaa
      421 accggaaaat ggtattgcat tactaaccgt tgaattggaa aacccgacag gctacggacg
      481 aattattcgt gaaaatggtt ctgtggtagc gattgttgag caaaaagatg ccaatgaagc
      541 acagcttaaa gtcaaagaag tgaataccgg cgtgatggta gcaagcggtg cgagcttcaa
      601 aaaatggttg aaaaacctaa ataacaacaa tgctcaaggt gaatattaca tcactgatgt
      661 gatcgcaatg gcaaaccaag aaggctttaa agtacaagcg gttcaggcaa ctgaatttat
      721 ggaagtagaa ggggcaaaca accgtttgca acttgctgca cttgaacggt tctaccaaaa
      781 acgtgaggcg gaaaaactgt tattggctgg tgtaactatt atcgacccaa gccgttttga
      841 tcttcgtggc actgtcaccc acggaaaaga tgttcagatc gatgtgaatg tcattctaga
      901 aggtgaaatc aaactcggta acaatgtaaa aattggtgca ggctgcgtac tgaaaaactg
      961 cgaaattggc gataatgttg aaattaaacc ttattctgtg attgaagatt cgattgtcgg
     1021 ggcaaaatcc gctatcggtc cattctctcg cttacgcccg ggggcagaat tagcggaaga
     1081 aacccatatc ggtaactttg tggaaatcaa aaaagctacc atcggcaaag gttcaaaagt
     1141 aaatcatctc acttatgtag gcgatgcgga aatcggcaaa gaatgtaaca tcggagcagg
     1201 tgtaatcact tgtaactatg atggtgcgaa taaattcaaa accattattg gcgataacgt
     1261 atttgtcggc tcagacagcc aactcattgc cccggtcacg attgccagcg gttcaaccat
     1321 tggtgccggt gcaacagtca ctaaagacat tgcggaaaat gaactggtaa ttagccgtgt
     1381 accacaacgc catattcaag gctggcaacg cccgactaaa aagaaatagt gccagtggtc
     1441 aaaagttatt atatttaaaa attggctttg ccaataacag caaagccata cactttaaaa
     1501 aggataat
//
LOCUS       DANLXS010000295         1495 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.303, whole genome shotgun sequence.
ACCESSION   DANLXS010000295 DANLXS010000000
VERSION     DANLXS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.303"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(26..202)
                     /locus_tag="PXD71_002637"
     CDS             complement(26..202)
                     /locus_tag="PXD71_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292694.1"
                     /translation="MAYVRIYQGEAYGWCAEKGEANTECPEALLVSLTGDIFEAVGGD
                     KLKGAKKWQPYKKE"
     gene            216..473
                     /locus_tag="PXD71_002638"
     CDS             216..473
                     /locus_tag="PXD71_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292695.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFYNYQQFMPEVEAKESLR
                     EQEPELDDGYNEEEYSEEEYSDENDASDENDNYDETYDDNF"
     gene            611..766
                     /locus_tag="PXD71_002639"
     CDS             611..766
                     /locus_tag="PXD71_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292696.1"
                     /translation="MLFKEKGYDEFLTQKIAKGRAELESGKGIPLEQATLLVQSTIEE
                     TAQELDD"
     gene            820..978
                     /locus_tag="PXD71_002640"
     CDS             820..978
                     /locus_tag="PXD71_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292697.1"
                     /translation="MTEEYQNFLIEQVKQGLDDIENGRVISLEESRQLCQKTIYETLQ
                     ELQGAVYA"
     gene            971..1288
                     /locus_tag="PXD71_002641"
     CDS             971..1288
                     /locus_tag="PXD71_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6292698.1"
                     /translation="MLSRKEVLMTRRASMNLQQIIRNVVEYTYSYESGDKLSAEIHNT
                     LDNLSYFPRIGVIGSVENTRECFCRGYRIIYRETENQVQILAIIHCRQQYPTDYYRSA
                     FKA"
BASE COUNT          538 a          287 c          263 g          407 t
ORIGIN      
        1 cacgtttttg tgtcgtatcc atctgtcatt ctttcttgta aggctgccat tttttggcac
       61 ctttcagctt atctccgccg actgcttcaa aaatatcgcc ggttaaactc accaataagg
      121 cctcagggca ttctgtattt gcctctcctt tttcagcaca ccaaccataa gcctcacctt
      181 ggtaaatacg aacatatgcc acccctcaat tagggatgat atgccacgct gatcctgaat
      241 atgtaaaata caactacgcc ggactttggg aaaaaggcag cgatggcact tttttctaca
      301 attaccagca atttatgcct gaagttgaag ctaaagaatc actaagggaa caagaacctg
      361 aacttgatga tggatataac gaagaggaat atagcgaaga agaatattcc gatgaaaatg
      421 atgccagcga tgaaaacgat aactacgatg aaacttatga tgataatttt taaatattcc
      481 caaaccacac ttccaaaaac cctgctcttg acactctatt gctttaagcg taaattattc
      541 caatatttcg catttaggag aacataatcc ctatgcctga aagccttgta ataagtgaaa
      601 ggaacataaa atgctattta aagaaaaagg ctacgatgag ttccttactc aaaaaattgc
      661 aaaaggcaga gctgaattag aatccggaaa aggtattcct cttgagcaag ccacattgtt
      721 agttcaatca actattgaag aaactgctca agaactggac gattaacaat ttgtaacatt
      781 gctaatatat ctatatacaa ctaatgatag gagttatcca tgacagaaga ataccaaaac
      841 ttcttaatag agcaagtaaa acaaggactt gatgatatag aaaacggacg tgttatttca
      901 ttagaagaaa gccgtcaact gtgtcaaaaa accatatatg aaaccttgca agagctacag
      961 ggagccgtgt atgcttagtc gaaaagaggt gttaatgact cgcagagcaa gtatgaattt
     1021 acaacaaatc attcgtaatg ttgtggaata tacttatagc tatgaaagtg gtgataaatt
     1081 atctgcggaa attcataaca ctttggataa tctttcttat tttccacgaa ttggtgttat
     1141 aggttcagta gaaaatacta gagaatgctt ttgccgaggt tatagaatta tatatcgaga
     1201 aacagaaaat caagtgcaaa ttttagccat tatccattgt cgtcaacaat acccaaccga
     1261 ctattatcgc tctgcattca aagcataaca ggagctataa aatggaaata aaaagaaaaa
     1321 tgaccgtagc cgaattaaaa ctcaaggcaa tgctcgccaa gcctaccata gttcaataat
     1381 attaggtgca aaaaacagga gaaccacaat gtctctactt aaaaaagcac aaacgtttat
     1441 ctctattttt aatgaagtgc gagctaaaaa tgaagctcaa cgccagtctt caaac
//
LOCUS       DANLXS010000296         1475 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.304, whole genome shotgun sequence.
ACCESSION   DANLXS010000296 DANLXS010000000
VERSION     DANLXS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.304"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1435
                     /locus_tag="PXD71_002642"
     CDS             <1..1435
                     /locus_tag="PXD71_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292699.1"
                     /translation="FKLTLAALAVSIVLSGCKSSQGPSIVADSPPPKEITATDGNDKE
                     KTDNKEKKSAQPNKSDPQPDKPANAKDPVKPDAETNKPSKKDKEYTPPATEKIDNSSV
                     QPDKMVPNPIENKENDSNKPNPKESDSDNNDKVTEGNKPNAPTETVNKDKEAEKSKKE
                     NKEDSSNNTPNINFDGAKPIDTDSKGSPYRVINLNPRKSVVNASAMQKVDFDSFTDES
                     VENSPFVSSDTEKEYNNTLVFGLGAGDKDSYFKNLNQKGDKHLGIHSGVYEDANLEGN
                     TKKINYLYVNQPYSSYGALFTDQKDSHLFNVRLTAGRENGDSYAEYGVFTQSGKEAKW
                     NDNLIGDATYKGQAIARVIRQVNGEQVAGIPQFDGDVTLNLHLSKEWNESKLNGKIDS
                     QSVGVITLKESEIANPTALRSNIGFGEEVIVENKENLVGDYAVDFTGPQLEEAVGVVE
                     LENEAETFSENEVIKYNAVFGVSKAAK"
BASE COUNT          555 a          255 c          299 g          366 t
ORIGIN      
        1 attcaaactt actcttgcag ctttagctgt ttctattgtt ttaagtggct gtaaatctag
       61 ccaaggtcca tctattgttg cagacagccc tccacctaag gaaataacag ctactgatgg
      121 caacgataaa gagaaaaccg ataacaaaga gaaaaagtct gcgcaaccaa ataaatccga
      181 tcctcaaccg gataaacctg ctaacgcaaa agacccagta aaacctgatg cggagacaaa
      241 taaaccaagt aaaaaagata aagaatatac accacctgct actgaaaaaa tcgataactc
      301 atcagtacag ccggataaaa tggtgcctaa tcctatcgag aataaggaaa acgacagtaa
      361 taaacctaac cccaaagaaa gcgatagcga taataatgac aaagtcactg aaggcaataa
      421 accaaatgcc ccaacagaaa cagtaaataa agataaagaa gctgagaaaa gtaaaaagga
      481 gaataaagag gactcaagca ataatactcc taacattaat tttgacgggg caaagcctat
      541 agatacagat agtaaaggta gcccgtaccg agtaataaac ctaaatccga gaaaaagcgt
      601 agtaaatgca agtgctatgc aaaaagtaga tttcgactct tttacggatg aatctgttga
      661 aaattcacca tttgtcagta gcgatactga aaaagaatac aataatacac ttgtttttgg
      721 gcttggtgcc ggagataaag attcttattt taagaactta aatcaaaaag gggataaaca
      781 tttaggtatt catagtggcg tgtatgaaga tgcgaattta gaaggtaata ctaaaaaaat
      841 taactatctc tatgtaaatc aaccttattc gagctatggt gctttattta cggaccaaaa
      901 agacagccat ttattcaacg ttcgtctgac cgctggtaga gaaaatggag atagttatgc
      961 cgaatatggt gtattcacac aatcaggaaa agaagctaaa tggaatgata acctgattgg
     1021 tgatgccact tacaaaggtc aagctattgc tcgcgttatt cgtcaggtaa atggggaaca
     1081 agtcgcaggt attcctcaat ttgatggtga tgttacatta aatttacacc tttccaaaga
     1141 atggaatgaa agtaaactaa acggcaaaat tgatagccaa agtgttgggg taattacact
     1201 taaagaaagt gaaatagcta accctaccgc actccgctca aatattggtt ttggagaaga
     1261 agtcatagtt gagaataaag agaatcttgt gggtgattac gctgtcgatt ttaccgggcc
     1321 tcagcttgaa gaggcggtag gcgttgttga attagaaaac gaagccgaga cattctctga
     1381 aaatgaagtc attaaataca atgcggtatt tggcgtaagc aaagcggcga aatagtattt
     1441 tttgtatctt acaaagggct gttaagccct tttat
//
LOCUS       DANLXS010000297         1461 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.305, whole genome shotgun sequence.
ACCESSION   DANLXS010000297 DANLXS010000000
VERSION     DANLXS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.305"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(148..1383)
                     /locus_tag="PXD71_002643"
     CDS             complement(148..1383)
                     /locus_tag="PXD71_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HDL6292700.1"
                     /translation="MKINRIILQSCSECGQQVRVPLTSNGNAVCPVCHNILRKSNTWS
                     LHRSAFLALAILILLPFALTFPLMSIDLLGIKINATVWDGIWKMATEGYPYTAFMVLV
                     CAVIMPIAFVLLILTIQLQRIIRQRPRYTLIFLTKIKEWVMLDVYLVALAVAAFKIRD
                     YADLQFDINLIAFVLVTLLNTLLFIKTEPKQAWERFYPEYHALPFDHPSNPTLCPTCE
                     YCFDETILDLKGRQRCPRCESNLSISDNVKIQRVWACLIAGTIMMIPANIFPISTTEL
                     AGQVSADSLMSGVLLFMEMGSYTVAAIVFIASIAVPVSKITIIFYLLLAIHYKWKHPI
                     HWQMKLLHYVHFVGRWSMLDLFVLSLMMSLLERGQILSFSVGEAAFYFGAAVFLTMFA
                     SANLDSRMLWKIHTVQKTS"
BASE COUNT          443 a          283 c          304 g          431 t
ORIGIN      
        1 taaagactat tttatatttg ctaatttctt tctggctatc ggtgctacat tatcggctct
       61 ttcttactca atagggattg agattttggg ttacgtacta cttttcttac tttgcatgtt
      121 atgtattatt tatcggaaaa tctttattta ggatgttttc tggacagtat gaatcttcca
      181 caacattctg gaatctaaat ttgcagaggc aaacatcgtt aagaatacgg ctgctccaaa
      241 atagaaagcg gcttccccta ccgagaaact gagaatctgc cctcgttcga gtagcgacat
      301 catcaatgat aacacgaata aatccagcat tgaccagcgt ccgacaaaat ggacgtagtg
      361 tagtagtttc atttgccaat gaatcgggtg tttccacttg taatgaattg ccagcaagag
      421 ataaaaaata atggtaattt tgctcaccgg caccgcaata ctagcgataa acacaatcgc
      481 tgccacggtg tagctaccca tttccataaa cagaagcacg ccggacatta atgaatctgc
      541 cgaaacctga cctgccagtt cagttgtcga aatcgggaaa atatttgccg gaatcatcat
      601 aatagtccct gcaattaagc aagcccaaac ccgttgtatt tttacgttgt cggaaataga
      661 taaattgctt tcacaacgag ggcagcgttg ccgtcctttt aagtctaaaa tagtttcatc
      721 aaagcaatat tcacaggtcg ggcaaagtgt tggattgctt gggtgatcaa acggcaaggc
      781 gtgatattct gggtaaaaac gttcccatgc ttgttttggc tcggttttaa taaaaagtag
      841 ggtgtttaag agtgttacca acacaaaggc gattaagttg atatcgaatt gtagatcggc
      901 ataatctcga attttaaaag cggcaaccgc aagagcaact aaatagacat caagcattac
      961 ccattctttt attttagtta agaaaatcag cgtgtaacgg gggcgttggc gaataatacg
     1021 ttgtagctga atggttaaaa ttagcaatac aaatgcaatc ggcattatta ccgcgcagac
     1081 tagcaccata aaagcggtgt aagggtagcc ctccgttgcc atcttccaaa taccgtccca
     1141 tacggtagca ttaattttga tgccgagtaa gtcaatgctc atcaagggaa aagtaagtgc
     1201 gaagggaagc aaaattaaaa tggctaatgc caaaaaagca ctacggtgca agctccaagt
     1261 attgcttttg cgtaatatat tatggcagac cgggcaaacg gcattgccat ttgacgtgag
     1321 cgggactcgc acttgttgcc cacattcgct acagctttgt aaaatgattc tgtttatttt
     1381 cattttttga tcttctatta aattaggtgg ttattttcta ctttatcgta gagattgcaa
     1441 gtggttaaat ttggcaaaat t
//
LOCUS       DANLXS010000298         1458 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.306, whole genome shotgun sequence.
ACCESSION   DANLXS010000298 DANLXS010000000
VERSION     DANLXS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.306"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(32..742)
                     /locus_tag="PXD71_002644"
     CDS             complement(32..742)
                     /locus_tag="PXD71_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6292701.1"
                     /translation="MWKNRQLFFGYAENRLLLLSRSITGLLGILCGVYIIDHLVLSDV
                     DMIGKLTSFILIILSAIFLKERASLLQWILCLIAFIGALFIIKPAVDIRFMPYLVGVI
                     GSVFAAIAYLCLRLLAKTKKVESPNTIVFFFSAFSTLILLPFVAIDYVVLTNLQTIYL
                     VLAGVTSAVGQFCVTTAYKYAAAKDISIYSYASVLFSAILGLLFFEQYPDMLSWLGYA
                     IIFVSSWLMFVLTKKQSK"
     gene            complement(978..>1458)
                     /locus_tag="PXD71_002645"
     CDS             complement(978..>1458)
                     /locus_tag="PXD71_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL6292702.1"
                     /translation="KFAKVLMVAVPAFALAACSSSSDDAAANAAANAAANAAANAAGE
                     FGGLTAEQLKAQYNTVYFGFDSYAVEGEYRQLLDAHAQLLAKTQGNVTIAGHADERGT
                     PEYNIALGQRRADAVKGYLAAKGANNTSTVSYGEEKPAVLGHSEADYAKNRRAVLEY"
BASE COUNT          489 a          299 c          270 g          400 t
ORIGIN      
        1 agaaatgaat aaacgttatt ctaacaaaat tttactttga ttgcttttta gttaatacaa
       61 acataagcca gcttgagaca aaaataattg cataacctag ccaactcaac atatccgggt
      121 attgttcaaa gaacaacaac cctaaaattg cactaaatag cacagaggca taactataaa
      181 ttgagatgtc tttcgccgcc gcatatttat aggcggtagt tacacaaaac tgcccgaccg
      241 ctgaagttac ccctgctaac actaagtaga ttgtctgtaa attggttagc actacataat
      301 caatggcgac aaaaggcaat aatatcaagg tcgaaaaggc agaaaaaaag aaaacaatgg
      361 tattaggtga ttctactttt ttggtttttg ccaataacct taagcataaa taggcaatgg
      421 ctgcaaacac agagccaatc acgccaacca gataaggcat aaagcgaata tcaactgccg
      481 gtttgataat aaaaagtgcc ccgataaagg caataaggca taaaatccat tgcaggagag
      541 atgctctctc ttttaaaaaa atcgcagaaa gaataattaa aatgaacgac gtgagtttgc
      601 caatcatatc gacatcactt agcacaaggt gatcgatgat atatacacca cataaaatcc
      661 ccaataaccc ggtgatggaa cggcttaata ataacaaacg attttctgca taaccaaaga
      721 ataattggcg atttttccac acaatataac cgcttatcaa tgctgtgcgt tgcgaaaaat
      781 cgctttttgt gcaatcggta catcccccga aagacgaaga aaagcagcca taatcgcaaa
      841 gcttagtgcc gacaaaagca cacaaaaaat ggctttaaat aacggcgaga gcgaattcca
      901 aaaattcata gcataaaggc ttttaataaa aataaagccc tgattattca gggcttcaat
      961 ttagaatcaa ctaagaatta gtattctaat actgcacgac ggtttttcgc gtagtcagct
     1021 tctgagtgac ctaatactgc tggtttctct tcaccgtaag aaacagttga agtgttgtta
     1081 gcacctttag ctgctaagta acctttaact gcatctgcgc gacgttgacc taatgcgatg
     1141 ttgtactctg gagtaccacg ctcatcagcg tgaccagcaa tagttacgtt accttgagtt
     1201 tttgctaata attgagcgtg agcgtctaat aattgacggt attcaccttc aactgcatag
     1261 ctgtcaaaac cgaagtatac agtgttgtat tgtgctttta attgttcagc tgttaaacca
     1321 ccgaattcgc ctgctgcatt tgctgctgca tttgctgctg cgtttgctgc tgcgtttgct
     1381 gctgcatcat ctgatgagct gctacatgct gctaatgcga aagctggtac tgctaccatt
     1441 aatactttag caaatttt
//
LOCUS       DANLXS010000299         1456 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.307, whole genome shotgun sequence.
ACCESSION   DANLXS010000299 DANLXS010000000
VERSION     DANLXS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.307"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(75..539)
                     /locus_tag="PXD71_002646"
     CDS             complement(75..539)
                     /locus_tag="PXD71_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL6292703.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCGGIARN
                     NPEMIKDAIGDLIVVCIIKAKQEKQPIYPVQFENHRLNGYRFDISVAVHCLYDIERIK
                     DRGKYSRFFGILHHLALINRFTLNDCLAHAYDQIKDRKGEMQAGIWVKEDDL"
     gene            complement(523..726)
                     /locus_tag="PXD71_002647"
     CDS             complement(523..726)
                     /locus_tag="PXD71_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292704.1"
                     /translation="MTDTERLDFIAQRKLDVRYSDLVDEFIIASYTKPHEQYFHWARN
                     ADLRTAIDEAMKEMRGLNNATTN"
     gene            complement(723..1214)
                     /locus_tag="PXD71_002648"
     CDS             complement(723..1214)
                     /locus_tag="PXD71_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL6292705.1"
                     /translation="MNEDKFFSVDVSNDIHILNLHETLEQAKQSCLNGATEAYEFADD
                     MDDHESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERESELFGEAEQVIEPPTLVENNG
                     WISVKDRLPEIRKEVLITNNFIMIAQFNGESWFKPGGFLGIQPVYGVTHWQPLPEPPK
                     EEK"
BASE COUNT          400 a          293 c          268 g          495 t
ORIGIN      
        1 aagaagaaac cgaaactctt atagagtggc ggaatgcttt tctgcaagat gtttcggttt
       61 ttatagctta ggtactataa atcgtcttct ttaacccaaa tcccagcttg catttcgccc
      121 ttgcgatctt tgatttggtc gtaggcgtga gcgaggcagt cattaagcgt aaatctattt
      181 attaaggcta aatggtgtaa tatgccaaaa aaacggctat atttacctct gtctttaatg
      241 cgttcaatat catatagaca atgaacagcg accgaaatat caaatctgta accatttaat
      301 ctatgatttt caaattgtac agggtaaata ggttgttttt cttgttttgc tttaataata
      361 cagacaacaa tcaaatcccc aatcgcatct ttaatcattt caggattatt gcgggcaatg
      421 cctccgcaga gttcgccaaa ctcttccatc agtttgagca tttgtttttg tggggttgag
      481 ccgttaatta aattgcggtc ttccgcccat tgttcgatgt ttttaattag ttgttgcatt
      541 gtttagtcct ctcatttctt tcattgcttc atcaatcgcc gtgcgtaaat cggcatttct
      601 tgcccaatga aaatactgct cgtgcggttt ggtatagcta gcaataataa attcgtccac
      661 caaatcagaa tatcggacat ctaatttgcg ttgggcgata aagtctaagc gttcggtgtc
      721 ggtcattttt cctcctttgg tggttcggga agtggttgcc agtgggtaac accatagaca
      781 ggctgaatac ctaagaatcc acccggcttg aaccagctct cgccattgaa ttgagcaatc
      841 ataataaaat tgtttgttat caagacttct ttgcggattt ctggcaatct gtctttgact
      901 gaaatccagc cgttgttttc tacaagtgtt ggtggttcaa taacttgttc agcttcccca
      961 aataactctg attctctttc ttcatcggtt agtgggcgga tatctgattt tgctctacct
     1021 aaaacaacac cataaactgc atagggtaaa tcataggctt catagctttc gtgatcatcc
     1081 atgtcatccg caaactcata ggcttcagta gcaccattta aacagctttg ttttgcttgc
     1141 tctaaggttt cgtgcagatt taggatatgt atatcatttg atacatccac cgaaaaaaat
     1201 ttgtcttcat tcattttctt tctccaaaat accaatagtt gagagaataa taagcatact
     1261 atctcatgct attcttaagt gcatctaacc acgctccagc atcttcggct gaagcgaaat
     1321 ataacccttg atcaaatagt ttttctatat aagaatgtgt agggaaattt gattttatca
     1381 cattgaaatt atcgccaata aaccacattc catctcttgg ttcttttaat ggacaaggaa
     1441 gagtgagagt tactgt
//
LOCUS       DANLXS010000300         1419 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.308, whole genome shotgun sequence.
ACCESSION   DANLXS010000300 DANLXS010000000
VERSION     DANLXS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.308"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            4..1320
                     /gene="argA"
                     /locus_tag="PXD71_002649"
     CDS             4..1320
                     /gene="argA"
                     /locus_tag="PXD71_002649"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDL6292706.1"
                     /translation="MQNTELALAQWFRQSTPYVNMHRGKTFVIMLDGDVIESPNFVNI
                     INDISLLHSLNIKLVIVFGARPQINQLLKQNHIEPEYYHNIRVTNPQSLEYVKQAVGK
                     VHYDFFARLSLRLPHSEVINVVSGNMILAQPIGVVDGVDYTLSGKIRRINIESIKQQL
                     AQNSIVLLGPIAPSVTGEMFNLPFEDIATQVAIKLRADKLIGFCEEQGILDENGKPIP
                     DYLPQDGETLLQQLIERGEYHTPKARFLQAANTVCREGVKRSHLLSYKEDGSLLQELF
                     SRDGIGTQFSMESSEQVRIANSSDIPGLLDLIRPLEQQGILVKRSREQLEMEITNYTI
                     IERDGVVIACAALNPYPEEKMAEMACVAVHPDYRDSSRGDILLENIQKRAVQLGMEKL
                     FVLTTRTTQWFQERGFRLAGAQDLPRYKRENYNYQRRSKVLILPLW"
BASE COUNT          434 a          271 c          317 g          397 t
ORIGIN      
        1 tatatgcaaa acacagaact cgcattagct caatggttta ggcaatctac accttatgtt
       61 aatatgcaca gaggcaaaac ctttgtgatt atgcttgatg gcgatgttat cgaaagcccg
      121 aattttgtca atattatcaa tgacatcagt ctcctgcaca gcctgaatat caaattagtg
      181 attgtatttg gggcaagacc gcaaattaac caattgttaa aacaaaatca tattgagccg
      241 gagtattacc acaatatccg tgtgactaat ccacaatcgc tagaatatgt gaaacaagcg
      301 gtagggaaag ttcattatga tttctttgcc cgcctttctt tgcgtttacc ccattctgaa
      361 gtgattaatg tagtcagtgg taatatgatt ttagctcagc cgattggcgt ggtggatggg
      421 gtagattata cactaagcgg taaaatccgt cgtattaata ttgagagcat taagcagcaa
      481 ctggcacaaa attcgattgt attacttggt ccgattgccc cgtctgttac cggtgaaatg
      541 tttaatctgc cgtttgaaga tattgccaca caggttgcaa ttaaacttcg tgccgataaa
      601 ttaatcggtt tttgtgaaga gcaggggatt ttagatgaaa acggcaagcc aattccagat
      661 tatcttccgc aagatggcga aaccttgtta cagcagttaa tcgagcgtgg cgaatatcac
      721 accccgaaag ctcggttttt gcaggcggcg aacacggtat gccgagaggg agtgaaacgc
      781 tcgcatttat taagctataa agaagatggc tcattgcttc aagagttatt ctctcgtgat
      841 ggtattggca cacagttctc aatggaaagc tcggaacagg tacgtattgc taattccagt
      901 gatattccgg gattattgga tttaatccgc ccacttgaac agcagggcat tttagtcaaa
      961 cgctctcgag aacaattaga aatggaaatc acaaactaca ccattattga gcgtgatggt
     1021 gtggtgattg cttgtgcggc acttaacccg tatccggaag aaaaaatggc ggaaatggcg
     1081 tgtgtggcgg ttcaccctga ttatcgtgat tcctcaagag gggatattct actggaaaat
     1141 attcaaaaac gggcagtgca actgggaatg gagaaactct ttgtgctgac tacaagaacc
     1201 acacaatggt tccaagagcg aggatttagg ctggcaggtg cccaagattt acctcgctat
     1261 aaacgggaaa attacaatta tcaacgtcgt tctaaagtat taattttacc actttggtaa
     1321 aatattcaat gtttttgacc gcttgttacc aagagaacta taaaaggaga actatgaaaa
     1381 aacatcaacc catcaaaggg gctatggcaa ttattactg
//
LOCUS       DANLXS010000301         1399 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.309, whole genome shotgun sequence.
ACCESSION   DANLXS010000301 DANLXS010000000
VERSION     DANLXS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.309"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>1399)
                     /locus_tag="PXD71_002650"
     CDS             complement(<1..>1399)
                     /locus_tag="PXD71_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6292707.1"
                     /translation="KKLKELENKVSSLEQTNHVQQQDISRLNILLEQKNIEENRNLER
                     MISYQLGYALINACKSFSGFISLPSELLKIRKLAKDKKQQKLLPSSPVANTPKQQTAL
                     VTSAPARSQSIDLTRGLTLLDPISELCWADAFTGYPLVRKSYADHIAGSTCKFAFFES
                     AWQANKGSWIYAFTSPGLKHNNAQALLDAIQKLKDKSIPIIFWNKEDPMHYEMFKPIA
                     EKVDYVFTTDQLVVDKYKKELGHSNIWALPFAAPIKVTNPIGRFSLDTETVCFAGTYY
                     AQNHENRKKQMDMLLPALLAYNGCIYDRASKDTSGKYAYPEQYTHLIREGVNFNEIVK
                     LYKKFKVFLNVNTITQSTTMMSRRVYELLASGTPVVSTPSKAITAQFPGIVITVNNEK
                     EAELAVHKLLTDSYYWHKQSMLGIREVMSKHTYENRWEFACSVINNEPIKEKTPSVRI
                     IAVYHNYLDLAIYIDS"
BASE COUNT          428 a          250 c          230 g          491 t
ORIGIN      
        1 gagaatctat atagatagct aaatctagat aattatgata aaccgcgata attcgaacac
       61 taggtgtttt ttcctttatt ggttcattat tgattacact acacgcaaat tcccaacgat
      121 tttcataagt atgttttgac attacttcac gtatgcccag catggactgc ttatgccaat
      181 aatagctatc tgtcagtaat ttatggacag caagctcagc ttctttctca ttatttacag
      241 taataacaat gccagggaat tgtgccgtaa tagctttaga tggcgtagat acaacaggtg
      301 tgccgctagc tagaagttca tacacacgtc ttgacatcat tgtggttgat tgagtgatgg
      361 tattaacatt taaaaatact ttaaatttct tatacaactt aactatctca ttgaaattta
      421 ccccttctcg gataagatga gtgtattgtt caggataagc atatttgcca cttgtatctt
      481 tagatgctct atcataaata cagccgttgt aagccaataa tgcaggtaac aacatatcca
      541 tctgtttttt acgattttca tgattttgag cataatatgt tccagcaaaa caaactgttt
      601 ctgtatcaag agaaaatctg ccaattggat tggttacttt aattggggca gcaaaaggca
      661 aagcccaaat atttgaatgc ccaagttctt ttttatattt atctacaacc aattgatctg
      721 ttgtaaaaac ataatctact ttttctgcga ttggcttaaa catctcataa tgcattggat
      781 cttctttatt ccaaaaaatg ataggaatgc ttttatcttt tagtttctga attgcatcta
      841 acaatgcttg agcattatta tgttttaagc ctggtgaagt aaaagcatag atccatgacc
      901 ctttatttgc ctgccaagca ctttcaaaaa aagcaaattt acacgtgctg ccagcaatat
      961 gatcagcata agattttcta actaaaggat accctgtaaa ggcatctgcc cagcataatt
     1021 cagaaatagg atccagtagt gtcaatcctc ttgtcaagtc aatagattga gatcgggcag
     1081 gtgcacttgt aactaatgca gtctgttgct ttggtgtatt tgcaacaggg gaagatggca
     1141 ataatttctg ctgtttctta tccttagcta acttcctgat ttttaataat tctgaaggca
     1201 aggatataaa accactaaat gatttacaag cgtttattaa agcatatcct aactgataag
     1261 aaatcattct ttcaagattt ctattttctt caatgttctt ttgctctaac aaaatattta
     1321 atctggatat gtcttgctgt tgaacatgat tagtttgctc aaggctgctt actttatttt
     1381 ctagctcctt gagcttttt
//
LOCUS       DANLXS010000302         1385 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.310, whole genome shotgun sequence.
ACCESSION   DANLXS010000302 DANLXS010000000
VERSION     DANLXS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.310"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            265..1383
                     /locus_tag="PXD71_002651"
     CDS             265..1383
                     /locus_tag="PXD71_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL6292708.1"
                     /translation="MKKTLVALAVTAFAASASATTVYDAEGSKVDFDGSLRIVLDRTN
                     SKELDNHNVVKTKKDAHTGIRNTGSRFGVRVKHDLAQDFYALGRVEFRFNKNTSDDGF
                     GDLYTKRAYVGLGSKQYGELTFGRQVTIADDLSQSVDYNYGINPKGDYIQTDGNQVIR
                     YNYRGVENLHVSANYNFAQDRKDGKVLANKLQNAYGIGALYTVDNWDLRAAYGHTNYK
                     TATDSTKHRKDGVLGSVGYKFGDLKLSVDTGYSREKGLAVTDDNGVTTFYNGVKSNAF
                     YVSPNFEYQVVPASKVYGNYLYERVKSKSNGSTYAAGKEKTHGFLLGVDYQFHKQVVA
                     FVEGSYTRTKDYDKVTNGYEYSGKSTNKAIGVGMRVYW"
BASE COUNT          454 a          199 c          268 g          464 t
ORIGIN      
        1 tgttatgcgc ttcatttttt ctgttaaaaa tgagtggatt ttctccaaat gaaataatgt
       61 aagtataacc atttgatttt aaaagaaaaa atgccgtctt tgttttgggg tttgaactat
      121 tgacattgag ttttcttttt gcaattattc tggcgaaaac aatgttaggc gttttgctga
      181 tacattgtat cttggataat tactcttaca ccttaatgag ggtaattact tatcatataa
      241 catataaaat ctaaggtgaa aattatgaaa aaaacattag ttgcattagc agtaaccgcg
      301 ttcgcagcgt cagcatcagc aaccaccgtt tacgatgcag aaggttcaaa agtagatttt
      361 gatggttctt tacgtattgt tcttgataga acaaattcta aagagctaga caatcataat
      421 gttgttaaaa ctaaaaaaga tgcacacaca ggaatccgta acactggttc acgttttggt
      481 gtaagagtaa aacatgattt agctcaagat ttctatgcat taggtcgtgt tgagttccgt
      541 tttaacaaaa ataccagtga tgatggtttt ggtgatttat atactaaacg tgcttatgtt
      601 ggtttaggta gtaagcaata tggtgaatta acttttggtc gtcaagtaac gattgctgat
      661 gatttaagtc aatcagtaga ttacaactac ggtattaatc caaaaggtga ttatattcaa
      721 accgatggta accaagttat tcgttataac taccgtggtg ttgaaaactt acatgtaagt
      781 gcaaactata actttgcaca agatcgtaaa gatggtaaag tattagcaaa taaacttcaa
      841 aatgcatacg gcattggtgc attatacact gtagataact gggatttacg tgcagcttat
      901 ggtcatacaa actacaaaac agctactgat agcactaagc accgtaaaga tggtgtatta
      961 ggttctgttg gttataaatt tggtgactta aaattaagtg ttgatacagg ttattctcgt
     1021 gagaagggtt tagctgttac ggatgataat ggtgtaacta cattctataa tggtgttaaa
     1081 tcaaacgctt tctatgtatc tccaaacttt gaatatcaag tagtgcctgc atctaaagta
     1141 tatggtaact acttatatga gcgtgtgaaa tctaaatcaa atggttctac atatgctgct
     1201 ggtaaagaaa aaactcatgg tttcttactt ggtgttgatt accaattcca taaacaagta
     1261 gttgcttttg ttgagggttc ttatactcgt actaaagact atgataaagt aactaatggt
     1321 tatgaatact caggcaaatc aactaacaaa gctatcggtg ttggtatgcg tgtatactgg
     1381 taatc
//
LOCUS       DANLXS010000303         1376 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.311, whole genome shotgun sequence.
ACCESSION   DANLXS010000303 DANLXS010000000
VERSION     DANLXS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.311"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1259
                     /locus_tag="PXD71_002652"
     CDS             <1..1259
                     /locus_tag="PXD71_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDL6292709.1"
                     /translation="LSISGSNQAFANWAQKKVKGKRGAKLMAAGLVFFTFIDDYFHSL
                     AVGAIARPVTDKFRVSRAKLAYILDSTAAPMCVLMPISSWGAYIITLIAGLLATHSIT
                     GYSPLGAFMTMSAMNFYAIFAMIMVFFVAYFSFDIGSMARFERQAAHLAELDDEQIET
                     KGHVRDLILPIIALIVATVSMMMKTGADALAESGASFSILGAFENTTVGISLVVGGVA
                     GLIVATICIITSGNISFKNYVRAYGLGVKMMSGAITILFFAWTINGVVSDMQTGKYLS
                     GLVAESIGAEFLPAILFVLAAAMAFSTGTSWGTFGIMLPIGASMAVHSDPALIIPCLS
                     AVMAGAVCGDHCSPVSDTTILSSTGAQCNHMDHVISQLPYALLVATASIIGYLVVGFT
                     QSAVLGFIATAVVMAILILLFKEKKH"
BASE COUNT          369 a          236 c          306 g          465 t
ORIGIN      
        1 tattaagtat ttcaggtagt aatcaggcgt ttgcgaattg ggcgcagaaa aaggtaaaag
       61 gcaaacgagg cgcaaaatta atggcggcag gtttggtttt ctttaccttt attgatgact
      121 atttccacag tttagcagta ggtgcgattg cccgccctgt tacggataaa ttccgtgttt
      181 ctcgtgctaa attagcttat attttagatt ctaccgccgc tccaatgtgc gtgttaatgc
      241 caatttcaag ctggggggct tatattatta ccttaatagc tggcttattg gctacgcatt
      301 ccatcacagg ctattcgcca ctaggcgcat ttatgacaat gagcgcaatg aacttttacg
      361 ccatttttgc gatgattatg gtgttctttg tggcatattt ctcatttgat attggttcga
      421 tggcgcgttt tgaacgtcaa gcagcgcatt tagcagaatt agatgatgag caaattgaaa
      481 cgaaaggaca cgtgcgcgat ttgattttgc caattattgc acttattgtt gctacagtct
      541 caatgatgat gaaaacaggt gcagatgcac tggccgaatc aggtgcttca ttttctatac
      601 taggtgcttt tgaaaatact acggtaggca tttcattagt tgttggtggt gtggcagggc
      661 ttatagttgc aactatttgt atcattacaa gcggtaatat ttcttttaaa aattacgtcc
      721 gtgcttatgg cttaggtgtg aagatgatgt ctggtgcaat cactattctc ttctttgcgt
      781 ggacaataaa cggcgtagta agtgatatgc aaacagggaa atatctatca ggattggttg
      841 cagaaagcat tggagcagag tttttacctg caattctatt tgtgttagcc gcagcaatgg
      901 cgttttcaac aggaactagc tggggaacat ttggtattat gttgccaatt ggagcctcaa
      961 tggcagtgca ttcagatcca gcattaatta ttccttgttt atctgcagta atggctggtg
     1021 cggtttgtgg tgatcactgt tctccagtat ctgataccac tattctttct tcaacaggcg
     1081 cacaatgtaa tcatatggat catgtcattt ctcaattacc ttatgcttta ttagttgcaa
     1141 cagcttctat tattggttac ttagtggttg gctttaccca atcagcggta ttgggattta
     1201 ttgctacagc tgttgttatg gcaatactca ttttgctttt taaagaaaaa aagcattaat
     1261 ttccgaaaat gtgaagggaa tcacagaatg agatttattg atgagataaa atagcactca
     1321 agatatggat atcgaatgtt gtgttatata aatcttaaaa taagatcctc ctagca
//
LOCUS       DANLXS010000304         1365 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.312, whole genome shotgun sequence.
ACCESSION   DANLXS010000304 DANLXS010000000
VERSION     DANLXS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.312"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>1365)
                     /locus_tag="PXD71_002653"
     CDS             complement(<1..>1365)
                     /locus_tag="PXD71_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292710.1"
                     /translation="YSRVGYDKGVWEALSVLLPERFDFEVFEVIDYSKEWSWFEIIVE
                     NLFWRQPEKINMQKLHQFFKNSPYFNQATWINILYQLATEINHPLNIKYLHNKLYSFS
                     LADRDEIWTAFIALESDQCPAIERLLTWCQKSSGQENIEPEALLLGAIAVSWLFTSTD
                     KRSRNGYINALAHLLWNRLEIALALFKEFIEVNDPYVMEGILSAIHGAVAYSENSSGL
                     KALALEIDNQIFNQPETEEIYPNVLVRDYARHIIEYSLQKDEYSSDDIEIIRERITPP
                     YRSSFPQNLPTNEEIDKKYHSDGQRIIIRSMTTEYGRGMCGYGDFGRYTFEAKFRNWE
                     SYKFGNLINVDLLSNYACQMIFEQFGYDDKKHLEFDKETRSRYWRRDDTNKIERIGKK
                     YQWLALYEILARVMDNFPTFSNRYADEIGLEYITNLDNFYLPKTQIKIVPPTKTDVFF
                     QYIN"
BASE COUNT          451 a          252 c          196 g          466 t
ORIGIN      
        1 gatttatgta ttgaaaaaat acatctgttt tagttggcgg tacaatttta atttgggttt
       61 tgggtagata aaagttgtcc aaattagtaa tatattcaag ccctatttca tcggcatagc
      121 gattagaaaa agtaggaaaa ttatccatca cccttgccaa gatttcatac aaagccaacc
      181 attgatattt cttgccaatt cgctcaattt tattagtatc atctcttcgc caatatcttg
      241 aacgagtttc tttatcaaac tcaaggtgct tcttgtcatc atagccaaat tgctcaaaaa
      301 tcatttggca agcataatta cttaataaat caacattgat gagattgccg aatttataac
      361 tctcccaatt tcggaatttg gcttcaaaag tatatctgcc aaaatcacca tatccacaca
      421 taccacgacc atattcagtt gtcattgaac ggataatgat tctttgtcca tcagaatgat
      481 attttttatc aatttcttca ttggttggta agttttgggg aaatgaactt ctataaggtg
      541 gcgtaattct ttctctgatt atctcaatat catctgatga atattcatct ttttgcaaag
      601 agtattcaat aatatggcga gcataatcac gaaccaaaac attgggataa atttcttctg
      661 tttcaggttg attaaatatt tgattatcta tctctaatgc aagtgctttt aagccagatg
      721 aattttcaga ataagcaaca gctccgtgaa tggcagataa aataccttcc ataacataag
      781 ggtcattaac ctctataaat tccttgaata acgccaatgc aatttccaat ctattccata
      841 ataaatgagc cagtgcatta atatagccat ttctacttcg tttatcggta gaagtaaata
      901 gccaactaac agcaattgca cccaataata gtgcttcggg ttcaatattt tcttgaccac
      961 ttgatttctg acaccaagtt agtaatcgtt caattgctgg gcattgatca gactccaaag
     1021 caataaaagc tgtccaaatc tcatctctat ctgccaaaga aaaagaataa agtttgttat
     1081 gtaaatattt tatatttaat gggtggttaa tttcggtagc cagttgatat aaaatattaa
     1141 tccaagttgc ttgattaaaa tatggactat ttttgaaaaa ttgatgtagt ttttgcatat
     1201 tgattttttc aggctgcctc caaaagagat tttctacaat aatctcaaac caagaccatt
     1261 cctttgaata atcaatcact tcaaatactt caaaatcaaa tcgttctggc aacaatacac
     1321 ttaatgcttc ccatacgccc ttatcatagc caactctact atata
//
LOCUS       DANLXS010000305         1339 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.313, whole genome shotgun sequence.
ACCESSION   DANLXS010000305 DANLXS010000000
VERSION     DANLXS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.313"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..514
                     /locus_tag="PXD71_002654"
     CDS             <1..514
                     /locus_tag="PXD71_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL6292711.1"
                     /translation="SKQIPYREYAVVLGTAKFYSKDVINQYYKYRLEAAKQLVQEGKV
                     NQLLLSGDNKTPYYNEPKTMTSDLLKMGIATSKIKQDYAGYTTFYSIIRAKKVYKLPP
                     FTIISQKFHCERALFIAKFRDIDAICYAATYPEGAYQVRMREILARSAMVFSLLIGKE
                     PESLEDIRSK"
     gene            complement(551..>1339)
                     /gene="purU"
                     /locus_tag="PXD71_002655"
     CDS             complement(551..>1339)
                     /gene="purU"
                     /locus_tag="PXD71_002655"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDL6292712.1"
                     /translation="LVAKITNICYKHQLNIIKNSEFVENETKRFFMRTELNGIFNDET
                     LLTDLKFTLPEGSSYKLLPKQRKRIVILVTKEAHCLGDLLMKNYYGGLDVEIAAVIGN
                     HETLKSLVERFDIPFHLVSHENLTRVEHDKLLAEKIDEYSPDYIVLAKYMRVLNPEFV
                     ARYPNRVVNIHHSFLPAFIGAKPYHRAYERGVKIIGATAHFVNDELDEGPIIMQNVIN
                     VDHTYTAEAMMRAGRDVEKTVLSQALELVLADKVFVYKNKTIIL"
BASE COUNT          428 a          227 c          256 g          428 t
ORIGIN      
        1 tagtaaacag attccttacc gggagtatgc agttgtactc ggtacggcaa aattttactc
       61 taaagatgtg attaaccaat attataaata tcgtttggaa gccgctaagc aattagtcca
      121 agaaggaaag gtcaatcagc ttttgttaag tggagataat aaaacgccgt actataatga
      181 gccaaaaaca atgacaagtg atctacttaa aatggggatt gcgacttcaa aaattaagca
      241 ggattatgcg gggtatacca ctttttattc aataattagg gctaaaaaag tatataaatt
      301 accgccattt acgattatct cccaaaaatt ccactgtgaa agggcattgt ttattgcaaa
      361 atttagggat attgatgcaa tttgttatgc cgctacatat ccagaggggg cttatcaggt
      421 acgaatgcgg gaaattttag ccagatcggc aatggttttt agtttgttga taggtaaaga
      481 gcctgaatca ttggaagata tccgttctaa atgagatagc cggtcagata atgttgaccg
      541 gctattttgc ttataaaata atggttttgt ttttatatac aaatactttg tcagcaagca
      601 ctaattctaa ggcttggcta agtacggttt tctcgacatc acgtccggca cgcatcatag
      661 cctctgcggt gtaggtgtga tcaacattaa tcacattttg cataataatc gggccttcat
      721 ctaattcatc attcacaaaa tgggcggttg caccgataat tttcactcca cgttcgtatg
      781 cacgatgata aggttttgca ccaatgaaag cgggtaagaa cgagtggtga atattgacaa
      841 ctcggtttgg ataacgagcg acaaattccg gattcagcac ccgcatatat ttagccaaca
      901 caatataatc aggtgaatat tcatcaattt tttccgctaa taatttatcg tgttccactc
      961 gggttaaatt ttcgtggctg actaaatgga atggaatatc gaaacgttca actaaacttt
     1021 ttaatgtttc gtggtttcca atcacggcag caatttctac atctaatcca ccatagtaat
     1081 ttttcatcag taagtcacct aaacaatgtg cttctttggt aactaaaata acgatacgtt
     1141 ttctttgttt agggagtaat ttatagcttg agccttcagg taaggtaaat tttaaatcag
     1201 tgagtaaggt ttcatcatta aaaattccgt ttaattcggt acgcataaaa aaacgtttag
     1261 tttcattctc aacaaattca gaatttttaa taatatttaa ttggtgctta tagcaaatat
     1321 tggtaatttt agcgacaag
//
LOCUS       DANLXS010000306         1324 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.314, whole genome shotgun sequence.
ACCESSION   DANLXS010000306 DANLXS010000000
VERSION     DANLXS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.314"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            32..751
                     /locus_tag="PXD71_002656"
                     /pseudo
     CDS             32..751
                     /locus_tag="PXD71_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            811..1260
                     /locus_tag="PXD71_002657"
     CDS             811..1260
                     /locus_tag="PXD71_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HDL6292713.1"
                     /translation="MLAKLKWNQLACVTLAFCSASVFSAHHEIKMLDNNAEGSMVFEP
                     GYVKAEVGDTVTFIPSHKGHWVESRSIPEGAEKFLSKEDEEFTITLTHEGVYVYYCPP
                     HRTMNMAGIIQVGQALNLEATQKEVEKIEKRTKENKGRLFEYLEQVK"
BASE COUNT          434 a          231 c          294 g          365 t
ORIGIN      
        1 gttttagtcg ttatctagga cagcccctac ttttaatact cgtaagcccc cttttgataa
       61 ccctaaagtt cgtcaagcat tgaattatgc ggtgaataaa actgaaattt taacttccgt
      121 ttaccaaggc tctgcaacgc cagctaaaaa cccaatgcct tctacgattt ggggatataa
      181 caacgaagta gccggttatg aatttaatcc ggaaaaggcg aagagcttgc ttaaagaggc
      241 cggattagaa aatggctttg aaacagaaat ttgggcaatg cctgtttctc gcccttacaa
      301 tccaaatgcc cgtagaatgg ctgaaatgat tcaagaggat tggaaagcaa taggggtaga
      361 ggcaaagata gtgagctatg aatggggaga atatcttaaa cgaatgggaa acggcgagca
      421 cgctaccgga atgattggtt ggaccggaga taacggcgat ccagataatt tcctcaatgc
      481 cctactcagt tgtaatgcgg tagaacaagg ctcaaactat gcagggtttt gtaatccaga
      541 gtttgaccgt ttaattaatg aggctgcgac tatcagtgat caaaacaagc ggtcagattt
      601 atataaaaaa gcgcaattca tctttaatga gcaagcacct tggttaccga ttgcacattc
      661 aacggtgtat tttccggtaa ggaaagaggt aaaaggctat gttgtcagcc cgtttattgc
      721 tcataatttt tatggtgttg agctggaata acctaaggta taatgaattg aactcatttt
      781 gtgcctcata aatttaggag caataaatat atgttagcga aacttaagtg gaatcaatta
      841 gcttgtgtaa cattggcatt ttgttcagcc tcagtatttt cagcccatca cgaaatcaaa
      901 atgttagata acaatgcgga aggatcaatg gtatttgagc ccggttatgt aaaagcagag
      961 gtgggcgata cggtaacatt tattccgtct cataaagggc attgggttga aagccgctcg
     1021 attccggaag gtgcggagaa gttcttatct aaagaagatg aggaatttac tattaccctt
     1081 actcacgaag gtgtttatgt ttactactgt ccgccacacc gaaccatgaa tatggccgga
     1141 attatccaag taggacaagc tctcaatctt gaagccacac aaaaagaagt ggaaaaaatt
     1201 gaaaaacgca cgaaagaaaa taaaggacgt ttatttgaat atttagagca agttaagtag
     1261 aagtgattta attttgcgat ctgcatcgaa aaatcaatat ttaatagttt acaaatgatg
     1321 ctta
//
LOCUS       DANLXS010000307         1276 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.315, whole genome shotgun sequence.
ACCESSION   DANLXS010000307 DANLXS010000000
VERSION     DANLXS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.315"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(186..527)
                     /locus_tag="PXD71_002658"
     CDS             complement(186..527)
                     /locus_tag="PXD71_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HDL6292714.1"
                     /translation="MKAILCQRAKQLRTNMTEAEKVLWQALRAKRFLAIKFKRQQVIG
                     NYIVDFVCFSAKLVIELDGSQHLDQQNYDNLRTHFLNGQGFRVLRFWNNQIHSDLEAV
                     LDTIFYEITRG"
     gene            complement(675..>1276)
                     /locus_tag="PXD71_002659"
     CDS             complement(675..>1276)
                     /locus_tag="PXD71_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HDL6292715.1"
                     /translation="SAWLILRDWFTQAKNHYEILASYPEDAGSALVGMQLSTRSPLGA
                     MVYETGGVFIDHGWLRILGSGNEKLPRGLFDWNFGKTFKQSGEQPSHLLVADDAIGGY
                     FAINAGGIGSDLGKVYYYHPKAKKWENTQLGYSEFLGWALAGDLNHFYQDLRWENWQT
                     DVEKLQGHQVLDLTSKIAVPIERHYQGEFAPENMGYSVA"
BASE COUNT          400 a          262 c          211 g          403 t
ORIGIN      
        1 cccacaaggg gagagggtta ttctttcaaa aattcaaaac aactaaaaaa ttagcattct
       61 ccttttgtag gagaaaatgt tgttctttca aaattctaaa ataaccgcaa aattaactct
      121 cgccccttgt gggagagaga cagataaatc attcgttcag aatgatttat cagagagagg
      181 ggaaattacc ctctcgtaat ttcataaaaa attgtatcta aaacagcctc tagatcgcta
      241 tgaatttgat tattccaaaa tcttaaaacc ctgaacccct gaccgtttaa aaaatgagtt
      301 cgtagattat catagttttg ttgatctaaa tgttgtgaac catctaattc aataactaat
      361 ttcgctgaaa agcaaacaaa atcgacaata tagttaccta taacttgttg ccgtttaaat
      421 tttatcgcta aaaatcgttt tgctcttaat gcttgccaaa gaaccttctc tgcttctgtc
      481 atattagtcc gaagctgttt agctctttgg cataaaattg ctttcatttt ttattcctct
      541 tttttaattt agtataaaag gaataaaaaa tataatttga gcgatttttg tgatctaaat
      601 cgaaaatcag aaaatttctt tcgttctaaa aaaataagct aaacagccta tatttcagcc
      661 tctcttacaa acacttaagc caccgaatat cccatatttt caggggcaaa ttcgccttgg
      721 tagtggcgtt caatcggaac ggcgatttta cttgtaagat ccagcacttg atgcccttgt
      781 aatttctcta catcggtttg ccagttttcc caacgcaaat cctgataaaa atggtttaaa
      841 tctccggcta atgcccagcc tagaaattct gaataaccta attgggtgtt ttcccatttt
      901 ttagcttttg gatgataata ataaactttg ccaagatctg agccgattcc gccggcatta
      961 atggcaaaat agccaccgat tgcatcatcc gctaccagca agtggcttgg ttgttcgcca
     1021 gattgtttaa aagttttgcc aaagttccaa tcaaataagc ctcgaggcaa tttttcgttg
     1081 cctgagccta aaatgcgtaa ccaaccgtgg tcaataaata cgccaccggt ttcatacacc
     1141 attgccccta gcggtgagcg agtagaaagt tgcataccaa ccaatgcact gccggcatct
     1201 tcagggtaac tcgctaaaat ttcgtaatga tttttggctt gggtaaacca gtctcttaaa
     1261 attaaccaag ccgatt
//
LOCUS       DANLXS010000308         1265 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.316, whole genome shotgun sequence.
ACCESSION   DANLXS010000308 DANLXS010000000
VERSION     DANLXS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.316"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..924
                     /locus_tag="PXD71_002660"
     CDS             <1..924
                     /locus_tag="PXD71_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL6292716.1"
                     /translation="DRMIKLDMIPKYYFKEIIDVLYTETFSRDNNLRADAPLETISEA
                     FKLDISKVYVALSKDLGITIKSPEQAATYVNDERYRRFNTLIDKKFNDSVLIELLNCF
                     EKRDDKRIEELVTDEAAIPTIFEYILGIIWYKVSERQGNILDFMKLSLEANLLPKTHA
                     AGGYADIIYEYEACTSYPKHSLLLEATLADGNNQRRMEMEPVSRHLGDYRIRFNNPFD
                     YSLFVSTYLDKNVISDFRYRKIIPYTRDEETITGMKIISMDTDSLKKIIENKVKYKYL
                     YEIFDKYHEMPLETVDWHDGMIKEATGEYKS"
     gene            986..>1265
                     /locus_tag="PXD71_002661"
     CDS             986..>1265
                     /locus_tag="PXD71_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDL6292717.1"
                     /translation="MKNRIEHPKIFISYAWGSQEHDEKVIALATNLKGDGIDVIFDKW
                     QLKEGNDTFKFMEKSVLDESITNVLILIDPIYAKKANERAGGVGTETQI"
BASE COUNT          477 a          152 c          256 g          380 t
ORIGIN      
        1 gatcgtatga ttaagttgga tatgattcca aagtattatt ttaaagaaat tattgatgtt
       61 ctgtatacag aaacttttag tagagataat aatctcaggg ctgatgcccc acttgaaact
      121 atatcagaag cattcaagtt agatattagc aaagtatatg tagctttaag taaagattta
      181 ggaattacaa ttaagagtcc ggaacaagct gcaacttatg taaatgatga acgatacaga
      241 agatttaata ctttgatcga taagaaattt aacgattctg tgcttataga gttactaaat
      301 tgttttgaga aacgtgatga taaaaggatt gaagaacttg ttacagatga ggctgcaata
      361 cctactattt ttgaatatat tttgggaatt atatggtaca aagtgagcga aagacaagga
      421 aatattttgg atttcatgaa gttatcatta gaagccaatt tattgccgaa gacccacgca
      481 gctggaggtt acgcagacat tatatatgaa tatgaagcgt gcacctctta tccgaaacat
      541 tcattattgc ttgaagcaac ccttgcagat ggaaataatc aaagaagaat ggaaatggag
      601 cctgtttcaa gacatttagg cgattatagg attagattta ataatccgtt tgattacagc
      661 ctatttgtta gcacatactt agataaaaat gtaatctcag attttagata tagaaagata
      721 attccataca ccagagatga agaaaccatt acaggaatga agattatttc aatggacaca
      781 gattctctta agaaaataat tgagaataag gttaagtaca agtatctgta tgaaatattt
      841 gataaatacc atgaaatgcc attagagact gtagattggc atgatgggat gataaaagag
      901 gctactggag agtataagtc atagtgatta taagatagtg agaaagaata cttattttga
      961 aaggaataca tgggaattga tacagatgaa gaatagaatt gaacatccta agatttttat
     1021 ttcatatgct tggggatcac aggaacatga tgagaaggtt attgctttag ctacaaatct
     1081 taaaggagat ggtatagatg tcatttttga taaatggcag ctaaaagaag gcaatgatac
     1141 ctttaaattc atggaaaaaa gtgttttaga tgagagtatt accaatgtac tgattcttat
     1201 agatcctatt tatgcaaaga aagcaaatga aagagccggt ggcgtaggta cagaaacaca
     1261 gatta
//
LOCUS       DANLXS010000309         1257 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.317, whole genome shotgun sequence.
ACCESSION   DANLXS010000309 DANLXS010000000
VERSION     DANLXS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.317"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..274
                     /locus_tag="PXD71_002662"
     CDS             <1..274
                     /locus_tag="PXD71_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HDL6292718.1"
                     /translation="FEMYLQECGQPYDKETINNLQYDFQGLGLHSLKKTMTNGKPDTI
                     NFWKCDVIGPRKTSSLTGYLIKNTHLLFGDKMLLNNHCLTLQEEDT"
     gene            314..1192
                     /locus_tag="PXD71_002663"
     CDS             314..1192
                     /locus_tag="PXD71_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL6292719.1"
                     /translation="MFFYRHYLRADTKRSYNNIIRIIKKRYPNLNANDVTTDMLIEWR
                     NQVVGNTIKAVTWNNYVRHLKALYNFAIAQGLLCMSSNPFNHLFIRESRRKKKTYSSE
                     QLHKIDYILHNNIILPSFLKPSWFILAIIMTLRCTGIRRGQLVKLKIGDVNLSNRTIY
                     ISPEINKNHDYHIVPISDSLYPHIERLIQELKIHKQTDDCQLFNLNLFSRYTRRKGQP
                     MSQNQLTHIFRHISDLVGFTSSPHRFRHTVATQLMKKPENVYVVKKLLGHKDISVTLG
                     YIEYDVEMLRDTVNLL"
BASE COUNT          442 a          191 c          218 g          406 t
ORIGIN      
        1 ttttgaaatg tatttgcaag aatgtggcca accttatgat aaggaaacta taaacaatct
       61 tcagtatgat ttccaggggc ttggtcttca ttctcttaaa aaaaccatga ctaatggtaa
      121 gccagataca ataaattttt ggaagtgtga cgtgattggt cctagaaaga cttcctcttt
      181 aacaggatat ttgataaaaa atactcatct cttatttggg gataaaatgt tactaaataa
      241 ccactgttta actttacagg aagaggacac ataatatgga ttctaagatt tccttcgata
      301 tattattgga acgatttttt tctatcgtca ttatttacgg gcggacacga aacgaagtta
      361 taacaatatt attcgaatta tcaaaaagcg ttatccaaac ttaaatgcga atgatgtgac
      421 tactgatatg ttaattgaat ggcgtaatca agttgttggt aatacaatta aagctgtgac
      481 gtggaataat tatgtgagac acctaaaagc attatataat tttgcgatag cacaggggct
      541 actttgcatg agtagtaatc catttaatca tttatttatt agagaatcta gaagaaaaaa
      601 gaaaacttat tccagtgagc agttgcataa aatcgattat attctacata ataatatcat
      661 cttacctagt tttttaaaac ctagctggtt tattcttgct atcattatga cattgagatg
      721 cacaggaatt aggagagggc aattggttaa actgaaaatc ggtgatgtta atttatcaaa
      781 taggacgatc tatatttcac cagaaattaa taaaaaccat gattatcata tagtaccgat
      841 atccgattca ctttatcccc atatagaaag gctgattcag gagttaaaga tccataaaca
      901 aacggatgat tgccagttat ttaacctcaa tttattttct cgttatactc gtaggaaagg
      961 tcagccgatg agccaaaacc agcttactca tatatttagg catatttccg atttggttgg
     1021 cttcacgtca tcaccacata gatttcggca tacagtagct actcaattga tgaagaaacc
     1081 tgaaaatgtt tatgtagtta agaaattatt aggtcataaa gatatcagcg ttacattagg
     1141 atatattgaa tacgatgtgg aaatgctacg agacaccgtg aacttactct aataaaagag
     1201 aaatgagaga aaactattct ctcatttatt gtgataaata ggaaacttct gtcttat
//
LOCUS       DANLXS010000310         1231 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.318, whole genome shotgun sequence.
ACCESSION   DANLXS010000310 DANLXS010000000
VERSION     DANLXS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.318"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            30..>1231
                     /gene="tolB"
                     /locus_tag="PXD71_002664"
     CDS             30..>1231
                     /gene="tolB"
                     /locus_tag="PXD71_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDL6292720.1"
                     /translation="MVADSDVRISIDEGVSMAQPIAVVPFKASGVPADVAQIISDDLR
                     NSGKFTPLERSQMPAQPGSAAEVVADQWSAIGIDNIVVGQVSSTGGGYNVAYQLIDTL
                     GTSGTAGGVIAQGSFIVPAAQIRQGAHTVSDEVFEKLTQIRGAFRTKIAYVVQRGVSS
                     YELRVSDYDGFNAFTITTSKEPLMSPEWSPDGSRLAYVTFENKKAQVVVHDIRSGARR
                     VVAALKGHNGAPSFSPDGSRIAFASNQDGELNIYVVGSGGGTPSKLTANAGNNTEPSW
                     SPDGSTIYFTSDRAGSPQVYRMSSSGGGASPMGGSGSYNAKVSSDGKNLIMIAGDRVV
                     KRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKGTSKVLQLVSADGRFKANLPGA
                     GGHYKFPA"
BASE COUNT          329 a          223 c          312 g          367 t
ORIGIN      
        1 gcatcttttc agcattattg acaactcagg tggttgctga ttctgatgtg cgaatttcta
       61 ttgatgaagg tgtaagtatg gctcagccga ttgcggttgt gccatttaaa gccagcggtg
      121 tgccggcaga tgttgcacaa attatttctg atgatttgcg aaatagtggt aaatttacac
      181 cgcttgaacg ctctcaaatg cctgctcaac cgggatcagc agcggaagtg gtggcagatc
      241 aatggagtgc tatcggtatt gataatattg tagttggcca agtgagtagc acaggtggcg
      301 gttataatgt cgcttatcaa ttaattgata ctcttggtac atcgggtaca gcgggtggtg
      361 tcattgccca aggttcattt attgttccgg cagcccaaat tcgtcaaggt gcgcatacag
      421 taagtgatga ggtatttgaa aaattaaccc aaattcgcgg tgcatttaga actaaaattg
      481 cgtatgtggt tcaacgtggc gtatcatcgt atgaattacg agtatctgat tatgatggtt
      541 ttaatgcctt tactattaca accagtaaag agcctttaat gtcgccagag tggtcaccag
      601 atggtagcag attagcttat gtgacatttg aaaataaaaa agcgcaggtt gttgttcacg
      661 atattcgttc aggtgctcgc cgtgtagtgg cagcattgaa aggacataat ggtgcgccat
      721 cattctctcc agacggttct cgcattgcgt ttgcctcaaa tcaagatggt gagttaaata
      781 tttatgttgt aggtagtggc ggtggtacac caagtaaatt gaccgctaat gctggaaata
      841 atactgaacc aagctggtct ccagatggta gcacaatcta ctttacgtct gaccgtgcag
      901 gttctcctca agtttataga atgagttcat caggtggtgg tgctagccca atgggcggta
      961 gcggtagcta taatgcgaaa gtatcatctg atggtaagaa tttaatcatg attgccggtg
     1021 atagagttgt gaaacgcgat ctcgcttcag gtggcacaga agtactgagt tcaactttct
     1081 tagacgaaag tccaagtatt tcaccgaatg gcattatggt tatttatagc tctaccaaag
     1141 gtacgagtaa agtgctacaa ttggtgtctg cagatgggcg ttttaaagct aatttgcctg
     1201 gcgcaggtgg acattataaa ttccctgcat g
//
LOCUS       DANLXS010000311         1228 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.319, whole genome shotgun sequence.
ACCESSION   DANLXS010000311 DANLXS010000000
VERSION     DANLXS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.319"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..525)
                     /locus_tag="PXD71_002665"
     CDS             complement(<1..525)
                     /locus_tag="PXD71_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292721.1"
                     /translation="MSALINLFYLYDPWFFHFLRMSFVVGLVACGYLVFQIIKGKSNG
                     IIIPKDSLIAIIALIGFSIIPLLINSTKEIGVLAMYVKMLILFVFGIAIYNVFYHNQQ
                     GKTLFVRDLKIGIAIQWICGVTALLGLPFMVDFLLSSNAMMPRFFGSEQEYRLYNITS
                     SAFFQLSIFYLMLLH"
     gene            complement(536..>1228)
                     /locus_tag="PXD71_002666"
     CDS             complement(536..>1228)
                     /locus_tag="PXD71_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL6292722.1"
                     /translation="AINTMSSDWEALNNHFDLALFEQRYGRKTTKGEVGCTLSHLAVY
                     QKIAEDSTIAEDDYCLVCEDDALFADDFQKHLDALLAQNLKVDIILVGQSKIPTFNDS
                     ELELNYPSTFGFLQKNIPNSQYKYAYSYLNYFAGTVAYLIKKSTACQFLAQCAGKNPY
                     WLADDFILFEKNFKFDVLIVRPLMAIENPVLISNLEDLRGSLNNNLFKKAVKYPLKKL
                     LAIKRNFTQRNS"
BASE COUNT          447 a          219 c          191 g          371 t
ORIGIN      
        1 atgtaatagc attaaataaa aaatgcttaa ttggaaaaag gcggaagaag tgatgttata
       61 taaacggtat tcttgttccg aaccaaaaaa acgtggcatc attgcattgc tggataataa
      121 aaaatccacc ataaacggca aacctaataa ggctgtcaca ccacaaatcc attgaatagc
      181 aatgccgatt tttaaatcac gaacgaataa agttttacct tgttggttat gataaaaaac
      241 attataaatc gcaatcccga acacaaacaa aatcagcatt ttgacataca ttgccagcac
      301 accaatttct tttgtactgt taatcaataa tggaataatg ctaaagccga ttaatgcaat
      361 aattgcaatc aggctatctt taggaataat aatgccattc gatttgccct tgattatttg
      421 gaaaaccaaa tagccacaag caacaagccc gactacaaac gacattctta aaaaatggaa
      481 aaaccacgga tcgtaaagat aaaataagtt aatgagtgct gacatttcag acctattatg
      541 aatttcgctg agtaaaatta cgtttaattg ccagtaattt tttaagtgga tattttacgg
      601 cttttttaaa taaattatta tttaatgaac cacgtaaatc ttccaaatta ctaattagaa
      661 caggattttc tatcgccatt aacgggcgga caattaacac atcaaatttg aaattctttt
      721 caaataaaat aaaatcatcg gcaagccagt atggattttt tccggcacat tgtgctaaaa
      781 actgacaagc ggtcgatttt ttaattaaat atgcaaccgt accagcaaaa taattcaggt
      841 aagaataagc atatttatat tggctattcg gaatattttt ttgcaaaaag ccaaaggttg
      901 atgggtaatt aagctctaat tcgctatcgt taaaagttgg aattttcgac tgcccaacta
      961 aaataatatc gacttttaaa ttttgtgcta ataaagcatc aagatgtttt tggaaatcat
     1021 cggcaaataa ggcatcatct tcacagacta ggcagtaatc atcttccgca atagtgctat
     1081 cttcggcaat tttttggtaa acggctaaat ggcttaaagt acaacctact tcgcccttag
     1141 tggttttacg cccatatcgc tgttcaaaca gagccaaatc aaaatggtta tttaatgcct
     1201 cccaatcact actcattgta ttaatggc
//
LOCUS       DANLXS010000312         1221 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.320, whole genome shotgun sequence.
ACCESSION   DANLXS010000312 DANLXS010000000
VERSION     DANLXS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.320"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            16..702
                     /locus_tag="PXD71_002667"
     CDS             16..702
                     /locus_tag="PXD71_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6292723.1"
                     /translation="MDTLASRLKIVLDQKGLTQEKFAEQIGVSQPSVFKILNGQTRNP
                     TRIYEIANVLDVDVNWLKTGKGEAPDFAKIGKNPTAYEEESSMLRLEVLDVYASAGNG
                     SFVTGDLTAYTHAVEFENAYFAQVFQRANAKGLSIINVDGDSMEPTIGNGDLLFVDTT
                     KSAYQGDGVYVFSYGENLYVKRLQFAGDELLVISDNPLYKEWRITSENEHKFQIHGKV
                     EFMQGHIRRV"
     gene            706..>1221
                     /locus_tag="PXD71_002668"
     CDS             706..>1221
                     /locus_tag="PXD71_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292724.1"
                     /translation="MEHQNLTHLVYITSHKQIGVYSISNLNIDDLYFKGYCIQKNRVI
                     TLRADRIIKQFDDLALAENYAKNIPQDVFYLFDSLLNQQRKEKITPIYKIGLCFTGFK
                     QARKNELIQLAVDNDLRVVQNVTGAVDFLIFDKESKTVGPAKLAKAEKLGIKIIDDEE
                     FLYMLETGVVPD"
BASE COUNT          419 a          199 c          243 g          360 t
ORIGIN      
        1 aataaggagt tcctaatgga tactttggca agtcgtttga aaatcgtttt agaccaaaaa
       61 ggtttaacac aggaaaaatt tgcagaacaa ataggtgttt cacagccatc tgtttttaaa
      121 atcctgaatg gacaaactcg aaatcccaca aggatttatg aaattgcaaa cgtcctagat
      181 gtcgatgtaa attggctcaa aaccggcaaa ggcgaagcac cagattttgc gaaaatcgga
      241 aaaaatccga ccgcttatga agaggaaagc tcaatgcttc gacttgaggt gttagatgta
      301 tatgcctctg ccggcaatgg tagttttgtc acgggagact taaccgctta cacgcacgca
      361 gtagaatttg aaaatgccta ttttgcacaa gtctttcaac gtgcaaatgc caaaggctta
      421 tcaattatta atgtagatgg cgactcaatg gagccaacta tcggtaacgg tgatttgctg
      481 ttcgtagata ccactaaatc cgcttatcaa ggcgatggtg tctatgtttt cagctatggc
      541 gaaaacttat atgtaaaacg cttgcagttc gccggtgatg aattgttagt catttccgac
      601 aatccgcttt ataaagagtg gagaatcaca tcagaaaatg agcataaatt ccaaattcac
      661 ggtaaagtgg aatttatgca agggcatatt aggagggttt aggtgatgga acatcaaaat
      721 ctaactcatc ttgtctatat tacatcacat aaacaaattg gagtttattc gatttctaat
      781 ttgaatattg atgaccttta cttcaaaggt tactgtattc agaaaaatag agttatcaca
      841 ttaagagctg atcgcattat taaacaattt gatgatttag ctcttgccga aaattatgcc
      901 aaaaatattc ctcaagatgt gttttatcta tttgattctc ttctgaacca gcaacgaaaa
      961 gaaaaaataa caccgatcta taaaatcggt ttgtgtttta ctggatttaa gcaagctaga
     1021 aaaaatgaat taattcaact agcagtagat aatgatttga gagtagtaca aaacgtaact
     1081 ggtgcagttg attttttaat tttcgataaa gagtcgaaaa ctgttggacc agcaaaacta
     1141 gcaaaagctg aaaagttagg aattaaaatc atcgatgatg aagaattttt atatatgtta
     1201 gaaactggcg ttgtgccgga t
//
LOCUS       DANLXS010000313         1195 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.321, whole genome shotgun sequence.
ACCESSION   DANLXS010000313 DANLXS010000000
VERSION     DANLXS010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.321"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..220
                     /locus_tag="PXD71_002669"
     CDS             <1..220
                     /locus_tag="PXD71_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDL6292725.1"
                     /translation="TLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIYSYISPKTGKR
                     STKNRIGSYPQMSLVEQSPQVFEQIARL"
     gene            272..859
                     /locus_tag="PXD71_002670"
     CDS             272..859
                     /locus_tag="PXD71_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HDL6292726.1"
                     /translation="MFDELKIAQATAYLLHKAGGTMEHIKVMKLLYLADRLSWQRYGY
                     SISNDEYYSLPYGPVLSNTLNLMRGEEASLYQKTSIWGEWIADKEDHKISLKKSVNTE
                     DEYFFDCLSESDTEILEEVFATFGNYKPFALVEYTHNPKYIPEWEDPQGRSKPISLST
                     LLTKLGKTPSEIESILEQQAEKGTMDNLFKGLGNA"
     gene            852..1169
                     /locus_tag="PXD71_002671"
     CDS             852..1169
                     /locus_tag="PXD71_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292727.1"
                     /translation="MLEIKRQVTILRPTNYGKEENHYKHLYILLTDPCFDGETGKGDM
                     ILSVNCSSIKDGKALDETCILKAGTHEFITRDSFIFYRHIKIDSLDEIKANIEAGYFI
                     QKP"
BASE COUNT          437 a          227 c          239 g          292 t
ORIGIN      
        1 cacattacta aaaaatctta aatattcccc aaatgcgaag cctatcgcag acagtaacgg
       61 gctatttatt cttgcacaga aaaaagcgaa agtatggatt tatagctata tcagccccaa
      121 aacaggcaag cgaagcacaa aaaatagaat cggtagctat ccgcaaatga gcttagtgga
      181 acaatcccca caggtatttg aacaaatcgc acgtctttaa aatattaaat taagagaaag
      241 cactatttgc gatgtagcag gagataaaac aatgtttgat gaattaaaaa tagcccaagc
      301 gaccgcctat ttgctacata aggcaggcgg aacaatggag catattaagg taatgaaatt
      361 gctttatctt gcggatcgct tatcgtggca aagatacggc tattctattt ctaatgatga
      421 atattattca ttgccttatg gaccggtatt aagcaacaca ctcaacctaa tgcgaggaga
      481 agaagcgagc ttataccaaa aaaccagtat ttggggcgag tggatagcag ataaggaaga
      541 tcacaaaatc agccttaaaa aatccgtaaa taccgaagat gaatacttct ttgattgcct
      601 ttcagagagc gacaccgaaa ttttagaaga ggtatttgcg acctttggaa attataagcc
      661 attcgcccta gtggaatata cccataaccc taaatacatt cccgaatggg aagacccaca
      721 aggcagaagc aagccgattt ctttaagcac gcttcttact aagctaggga aaacgccaag
      781 cgagatcgaa agcattttag agcaacaggc ggaaaaaggg acaatggata accttttcaa
      841 gggcttaggc aatgcttgaa atcaaaagac aagtaacgat tcttagaccg accaactacg
      901 gcaaggaaga gaaccactac aagcatttat atattctgct taccgatccg tgctttgatg
      961 gtgaaaccgg taaaggagat atgattttaa gtgtaaactg ctcaagcatt aaagacggga
     1021 aagcacttga tgaaacttgt attttaaagg caggaacaca cgaatttatc acacgggaca
     1081 gcttcatttt ctaccgccat ataaaaattg attcgttaga tgagattaag gcaaatatag
     1141 aagccggtta ttttattcaa aagccctaat taatgatgaa gcctatcaac aaata
//
LOCUS       DANLXS010000314         1124 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.322, whole genome shotgun sequence.
ACCESSION   DANLXS010000314 DANLXS010000000
VERSION     DANLXS010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.322"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1063
                     /locus_tag="PXD71_002672"
     CDS             <1..1063
                     /locus_tag="PXD71_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292728.1"
                     /translation="FILFDSILERHLGESLKRALESLGHQAVFTDLILHGHSMIYKQA
                     DIDFMWKKVKDICDEKADMFISFRPMNLTTEMLEYIGKRMKTAIWLSDDPVLYKTCYS
                     QVVNHYDVLLHCGYKEVMDFYEANNHPKGFNFPFWTDHVAFPSVYNPLHSDYEIAFLG
                     NMNGQVRRKRYMELASLPFTKKVFGLIDSDPLAMHGGFINEAYLHTKRVTEVLSKAKV
                     GVSIPQFFTEYNGLDYDYPELAGLGYFQFPSRVIQYAASGLPIAAVGDERMKEVYPEI
                     FVGNSITELEPYIKQICEDYDFALSESAKILTRFRKNYSALSRAMMLIDLVENLDKLQ
                     SQTTQERAILFTKYKAQYN"
BASE COUNT          377 a          161 c          218 g          368 t
ORIGIN      
        1 gtttatttta tttgattcta ttttagaacg tcatttgggc gaaagtttaa aaagagcttt
       61 ggagtcgtta gggcatcaag cagtatttac agatttaatt ttgcacggcc attctatgat
      121 ttataaacaa gcggacatag atttcatgtg gaaaaaagta aaagatattt gtgatgaaaa
      181 agcagatatg tttatctcat ttcggccgat gaatctaacg acagaaatgc ttgagtatat
      241 aggcaagcga atgaagaccg ctatttggct ttcagatgac cctgttttat ataaaacttg
      301 ctattctcaa gtagtaaatc attatgatgt attattacat tgcggatata aagaagttat
      361 ggatttttat gaagctaata accatccaaa aggctttaat tttccgtttt ggacagatca
      421 tgtagcattt ccaagtgtat ataacccgct tcattctgat tatgagattg catttttagg
      481 taatatgaat gggcaagtta gacgcaaaag atacatggaa ttggcttcat taccatttac
      541 caaaaaagta ttcggtttaa tagattcaga tccattagca atgcatggtg gatttatcaa
      601 tgaagcatat ttacatacaa aacgtgtaac agaggtttta tctaaggcaa aagttggggt
      661 gagtatccca caattcttta ccgaatataa tggtttagat tatgattatc ctgaattggc
      721 gggtttagga tatttccaat ttcctagtcg agttatccaa tatgctgcaa gtgggttacc
      781 tattgcagcg gttggtgatg agagaatgaa ggaagtttat cctgaaatct ttgttggcaa
      841 tagtattaca gagctggagc cgtacattaa acaaatttgt gaagattatg attttgcact
      901 atcagaatcg gctaaaattc taactagatt tagaaagaac tattctgcct tatcccgtgc
      961 catgatgttg attgatttgg tagaaaattt agacaaacta cagagccaga caacacaaga
     1021 gcgcgcaatt ttatttacta aatataaagc tcaatacaac taggagtaaa ctttatgaat
     1081 attaaagact tacttaaaga gatcagcttt aaagctgtaa ttaa
//
LOCUS       DANLXS010000315         1115 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.323, whole genome shotgun sequence.
ACCESSION   DANLXS010000315 DANLXS010000000
VERSION     DANLXS010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.323"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            159..434
                     /locus_tag="PXD71_002673"
     CDS             159..434
                     /locus_tag="PXD71_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6292729.1"
                     /translation="MAKATVARRIKNAINGNFGDHKSLAGTGGLYEMRIATGAGYRIY
                     YAQQGNVIYILLHGGDKSTQQADIEKAKALWAELKTTGESNGNNTNQ"
     gene            412..735
                     /locus_tag="PXD71_002674"
     CDS             412..735
                     /locus_tag="PXD71_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL6292730.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNNVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
BASE COUNT          400 a          214 c          220 g          281 t
ORIGIN      
        1 ctgctctctt ttcaaaattt aaccacttga ttatttgtat cttaatggat acactaagca
       61 ataaatagaa taagaggctg gcaatgacag agcaaaccac taatcaaatc atcattaccg
      121 ataatttttc aaaatagctt gagaatttaa agaaccctat cgcaaaagca acggtagcaa
      181 ggcggataaa aaatgcgatt aatggcaact ttggcgatca taaatcttta gccggtacag
      241 gcggacttta tgaaatgcgg atagcaacag gagccggata tagaatttat tacgcacaac
      301 aaggaaatgt aatttatatc ttgttacacg gcggcgataa atcaacacag caagccgata
      361 tagagaaagc gaaagcatta tgggctgaat taaaaactac aggggaaagc aatggaaaca
      421 atacaaatca atgaacaggc agcagaaaag cacggtttac gcccttttga cgtggcggag
      481 tttttaaccg atgaggcaac tattcaagcc tatttagcgg aagtgttaaa agagggcgac
      541 caagacgaat ttttagaagc cttgaacaat gtggcaaggg caaggggaat ggcggagctt
      601 gccaagcaaa cagggctagg gagagagagc ctatataaaa ccctagccaa aggcagcaaa
      661 ccacgctttg acactattca aaaaattcta ggggcattca atttagtgct tattccacaa
      721 gtcaaaaatg gctaaaactt atcacttgct acctgccaaa acctcacatt caaatagccg
      781 ttgattgata ttaacggctt ttttattgct taattctgta ccaattacgc accgattgcg
      841 gatcaatacg gggcaagttt tcgcaaaacc ttaatgaaat atgaccgctt gtgattttgt
      901 acaaaacgac acaaaccaat gtataaaatc aaaacttgcc acaaatggcg gaatgatatt
      961 aaatgcccca caaactagca gtatttctta atgacttttc attttaaggg tacatttaag
     1021 ggtatgttta acaagtctta aatgcacttc tcaataataa caagcctcat agcatacaat
     1081 tcaaactcgg ctggggacca tttaagcgtt gcata
//
LOCUS       DANLXS010000316         1054 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.324, whole genome shotgun sequence.
ACCESSION   DANLXS010000316 DANLXS010000000
VERSION     DANLXS010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.324"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..633
                     /locus_tag="PXD71_002675"
     CDS             <1..633
                     /locus_tag="PXD71_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL6292731.1"
                     /translation="TVFPKQVHFADYLNYAIFADFLEKNQVNYNLNIPNELSSFANTL
                     WQEKKIRLLLNPQGSMREISTQELSELLSMIDKRYVQHISCLLTNTTNSAEYLSKIKA
                     PFEISLAPNSDILGYCALVNSADIVIAVDGGGVHISCALGKKLLSFYANHPANIARWY
                     PKPKKDVETFMVIADKWTEDNNDTRGFSLVAPAKWLNHQFAKLIEKYTAC"
     gene            complement(688..900)
                     /gene="rpmE"
                     /locus_tag="PXD71_002676"
     CDS             complement(688..900)
                     /gene="rpmE"
                     /locus_tag="PXD71_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDL6292732.1"
                     /translation="MKQGIHPEYKEITATCSCGNVVKTRSTVGKDLNLDVCGNCHPFY
                     TGKQRVVDTGGRVERFNKRFSIPSSK"
BASE COUNT          341 a          186 c          192 g          335 t
ORIGIN      
        1 actgtctttc caaagcaggt tcattttgct gactatctca actatgctat ttttgctgat
       61 tttttagaaa aaaatcaggt caattataat ttaaacattc ctaatgaact aagcagtttt
      121 gcaaatacgc tatggcaaga gaagaaaatt cgattactat tgaatcctca aggtagtatg
      181 cgtgaaattt ctactcaaga attatcagag ctattatcta tgattgataa aagatatgtt
      241 caacatatta gttgtttgct gaccaataca accaatagtg ccgaatattt gtcgaaaatt
      301 aaagccccat ttgaaataag cctagcacct aatagtgata ttttaggtta ttgtgcatta
      361 gtaaactctg ctgatattgt aattgcagtt gatggaggtg gtgtccatat ttcttgtgca
      421 ttaggcaaga aattgctctc attttatgcc aatcatccag ctaatattgc acgctggtat
      481 ccaaaaccta agaaagatgt tgaaactttt atggtgatcg cagataaatg gaccgaggat
      541 aataatgata ctcgaggttt ttctctggta gctcctgcaa aatggttaaa tcaccaattt
      601 gcaaaattga tagaaaaata caccgcttgt taattcgata tataagaaaa acaaaacccc
      661 gcattagcgg ggtttgcaat aatcaaatta ttttgagctt gggatgctga aacgtttgtt
      721 aaagcgttca acacgaccac cggtgtcaac aacacgttgt ttaccagtat agaacgggtg
      781 gcagttgcca catacgtcta agtttaaatc tttacccact gttgagcgag ttttaactac
      841 attaccgcaa gagcaagttg ccgtaatttc tttatattct gggtgaatac cttgtttcat
      901 tggggaacct cataagaagc catatcgccg cctatcttgt tagaatctct accgctgata
      961 ggtaccatat gtaattaata aaaatgttga gcaaatgctc aacgccaaaa agagtgcgta
     1021 ttttagataa atttgttgcc tagatcaact acta
//
LOCUS       DANLXS010000317         1020 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.325, whole genome shotgun sequence.
ACCESSION   DANLXS010000317 DANLXS010000000
VERSION     DANLXS010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.325"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            276..803
                     /locus_tag="PXD71_002677"
     CDS             276..803
                     /locus_tag="PXD71_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL6292733.1"
                     /translation="MNEEKYFSVDVSDETHVIRLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERASELFGEAEQVIEPPTLLE
                     NNGWISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIA
                     VTHWQPLPEPPKEEK"
     gene            800..1003
                     /locus_tag="PXD71_002678"
     CDS             800..1003
                     /locus_tag="PXD71_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292734.1"
                     /translation="MTDTERLDFIAQRKLDVRYSDLVDEFIIASYTKPHEQYFHWARN
                     ADLRTAIDEAMKEMRGLNNATTN"
BASE COUNT          350 a          183 c          223 g          264 t
ORIGIN      
        1 gcagtaacat tgacgttacc ttgcccatta aaagaacctc aagaaaatat gtggtttatt
       61 gacaatgatt ttaccatagt aaaatctatg ttcgcaaatg ccccctttgt aaaaaaattc
      121 ttgccacaag ggcgatgttt cgcttcagaa gaagacgctc aagaatggtt agatgcaatg
      181 cgaaatagtc gtaggtaaat cggcagcact aacagctgaa tgctgctgtt gaatttcttg
      241 tatatccaca taaacacgga acgcggagaa agaaaatgaa tgaagaaaaa tatttttcgg
      301 tcgatgtctc ggatgagaca catgtaataa ggttgcacga aacattagag caagcaaaac
      361 aaagctgttt aaatggtgct actgaagcct atgagtttgc gggtgacatg gatgatcacg
      421 aaagctatga aagctatgaa gcctatgatt taccctatgc agtttatggt gttgttttag
      481 gtagagcaaa atcagatatt cgtccactaa cagacgaaga aagagcatcg gagttatttg
      541 gggaagctga acaagttatt gaaccaccaa cacttttaga aaacaacggc tggatttcca
      601 ttgaggacaa attaccacct attgaaacgg atgtgctagg gttatgcgat atttcaggaa
      661 tgcagttaat tttaatagtt tcaagagaac ttgccgataa cgagtggtat tttcttagtg
      721 taaatcaata tgggcttgat gacgatgtga ttgcagtaac ccactggcaa ccacttcccg
      781 aaccaccaaa ggaggaaaaa tgaccgacac cgaacgctta gactttatcg cccaacgcaa
      841 attagatgtc cgatattctg atttggtgga cgaatttatt attgctagct ataccaaacc
      901 gcacgagcag tattttcatt gggcaagaaa tgccgattta cgcacggcga ttgatgaagc
      961 aatgaaagaa atgagaggac taaacaatgc aacaactaat taaaaacatc gaacaatggg
//
LOCUS       DANLXS010000318         1014 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.326, whole genome shotgun sequence.
ACCESSION   DANLXS010000318 DANLXS010000000
VERSION     DANLXS010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.326"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>1014)
                     /locus_tag="PXD71_002679"
     CDS             complement(<1..>1014)
                     /locus_tag="PXD71_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6292735.1"
                     /translation="DYFISEDINEKMISSQLKEGDVLVNIVGATLEVVGRVAYVSSTF
                     PKANITQAMSFVRLKSKYNKELLPTYLFAFLQSSYGKIQINRNARPTGQYNLNNEELG
                     AIKVPLIDLETQKQVDKIIKQSNDFVQKSTQSYQEAETLLLENLGLRAFQADSNPVNV
                     KSLKESFLQTGRLDAEYYQTKYEQYWNLIQSQDYVFIRDEYLHITQKPDWTKPMYQYI
                     EIGDVNIGDGSYQTNWIETQELPANAKTQAQTGDILISTVRPYRGAVTIIGENDQDLV
                     VSGAFTVLRRKENSVFNNEVLKVLLRSELYKDWLLQFNVGTSYPVIKDNDILNLPIPK
                     ISGE"
BASE COUNT          316 a          181 c          141 g          376 t
ORIGIN      
        1 ttctccactt attttaggaa ttggtaaatt caaaatatcg ttatctttaa ttacaggata
       61 actcgtacct acattaaatt gtagtaacca atctttatat agttctgacc gtaatagcac
      121 ctttaaaact tcattattaa aaacagagtt ttcctttctg cgtaatactg taaacgcacc
      181 actaacaact aaatcttgat cattttcacc gataattgtt actgcaccac gataagggcg
      241 aacggttgaa attaaaatat cacctgtttg ggcttgagtt ttagcattag caggtaattc
      301 ttgtgtctct atccaattcg tttgatatga accatcacca atattaacat caccaatttc
      361 aatatattga tacatcggtt ttgtccaatc aggtttttgg gtgatatgta aatattcatc
      421 acgaataaaa acataatctt gcgattgaat taaattccag tattgctcat atttagtttg
      481 ataatactct gcatctaatc gtcctgtttg taagaaactc tcttttaagg atttaacatt
      541 tacaggattg ctatcagctt gaaaagctct caaaccgaga ttttctaata ataaagtttc
      601 agcttcttgg taactttgtg ttgatttttg tacaaaatca ttactttgct taatgatttt
      661 atctacctgc ttttgtgttt ctagatcaat taaggggact ttaatcgcac caagctcctc
      721 attattcaga ttgtattgtc ctgttgggcg tgcattccgg ttaatttgga tttttccata
      781 agaactttgt aaaaaagcaa ataaatacgt tggtaataat tctttattat attttgattt
      841 taatcttaca aaagacattg cttgtgtaat atttgctttt ggaaaagtgc tactaacata
      901 agctacccga ccaacaacct ccaaagttgc accaacgata tttactaaaa catccccttc
      961 tttcagttgt gatgaaatca ttttctcatt gatatcttcg ctaataaaat aatc
//
LOCUS       DANLXS010000319         1007 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.327, whole genome shotgun sequence.
ACCESSION   DANLXS010000319 DANLXS010000000
VERSION     DANLXS010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.327"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            73..318
                     /locus_tag="PXD71_002680"
     CDS             73..318
                     /locus_tag="PXD71_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292736.1"
                     /translation="MTNDGSIIARGSGFTTYALELENDGLKYLVGFVQPNGNAKEFIE
                     PGATIRRSNDQPSIGSVDCDPRKKIHYKFDVHLMNTL"
     gene            537..>1007
                     /locus_tag="PXD71_002681"
     CDS             537..>1007
                     /locus_tag="PXD71_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4852 domain-containing protein"
                     /protein_id="HDL6292737.1"
                     /translation="MCKKAFPLWGLIFGLLLSTSAFSVSKNFNYQNILMESLHFSENI
                     ELSSLSKDWLLYSDRNTYHKYQNDEFEFQDKIEQAGKKLQKLVDDRKSEEAPIVFTIP
                     VNSEFGNYDFEQGKFEYQPLMNGHMYISSNQYTENLPRKINLYVSNPEFIDGIPM"
BASE COUNT          342 a          164 c          194 g          307 t
ORIGIN      
        1 gttaaacggg taggaaatga ttacgagtat tcgtatgaca aaactacttt caaaaacccg
       61 attaaaaagg cagtgaccaa cgacggttcg attatcgcca gaggctcagg ttttactact
      121 tatgccttag agttggaaaa tgatggcttg aaatatctag tcggatttgt tcaaccaaat
      181 ggaaatgcaa aagagtttat tgagccggga gcaacaattc gccgtagtaa tgatcaacca
      241 agtattggtt ctgtggattg tgaccctcgt aaaaaaatcc attataagtt tgatgttcat
      301 ttgatgaata ctttataata cctttgtggt gctagagcta gcccaagtta aaaaagacaa
      361 agctcggcag atgaacaatt gtggtgtcag agctgaccca cgttaaaaaa cacaatgctc
      421 ggtaggtaaa tcccttaggt ttaaaaaatc taagggattt ttgtttaaaa gacttgctct
      481 ttattatcag gtggatctat cataatttaa actctctaat gtgtcaggag ataaaaatgt
      541 gtaaaaaagc tttcccatta tggggcttaa tcttcggatt actgctatcc acttcagcat
      601 tctcagtgtc taaaaacttc aactatcaaa acatattgat ggaatcatta cattttagtg
      661 agaatatcga gcttagttcg ttatctaaag actggttact ttacagcgat agaaatacgt
      721 atcataaata tcaaaatgac gagtttgagt ttcaggataa aattgagcaa gctggtaaga
      781 aactacaaaa actggtggat gacagaaaat ctgaagaagc ccctattgtt ttcacaattc
      841 ctgtaaactc agaattcggg aattatgatt ttgaacaagg taagtttgag taccaacccc
      901 tgatgaatgg gcatatgtat atttcttcta atcaatatac tgaaaatttg ccaagaaaaa
      961 ttaatctgta tgtttctaat cccgagttta ttgacggtat acctatg
//
LOCUS       DANLXS010000320         1000 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.328, whole genome shotgun sequence.
ACCESSION   DANLXS010000320 DANLXS010000000
VERSION     DANLXS010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.328"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            7..954
                     /gene="cysK"
                     /locus_tag="PXD71_002682"
     CDS             7..954
                     /gene="cysK"
                     /locus_tag="PXD71_002682"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL6292738.1"
                     /translation="MTIYADNSYSIGNTPLVRLKNFGNGNIVVKVEGRNPSFSVKCRI
                     GANMVWQAEKDGILTKDKEIVDATSGNTGIALAYVAAARGYKLTLTMPETMSTERKRL
                     LRGLGVNLVLTEGAKGMKGAIAKAEEIAASDPDRYVTLKQFENPANPAIHQQTTGPEI
                     WDATEGKIDVLVAGVGTGGTITGVSRAIKQDKGKQIISVAVEPSESPVITQTLNGEEV
                     KPGPHKIQGIGAGFIPKNLDLSLLDRVEQVSSEEAITTARRLMAEEGILGGISSGAAV
                     AAADRIAKLPEFADKLIVVILPSASERYLSTLLFEGIEA"
BASE COUNT          309 a          198 c          243 g          250 t
ORIGIN      
        1 atcactatga ctatctatgc agacaactca tactcaatcg gcaatacccc gctggtgcgt
       61 ttaaagaatt ttggcaacgg caatattgtg gtaaaagtgg aagggcgtaa cccaagtttt
      121 agcgtgaaat gccgtattgg tgcgaatatg gtgtggcaag cagagaaaga cggtattcta
      181 accaaagaca aagaaattgt agatgcaacc agcggtaaca ctggaattgc cttggcttat
      241 gtagcagccg ctcgtggtta taaattaacg ctcacaatgc cggaaacaat gagtaccgag
      301 cgtaaacgct tattgcgtgg cttaggagta aatttagtct taaccgaagg ggcaaaagga
      361 atgaaaggag cgattgcgaa agctgaagaa atcgcggctt ccgacccaga tcgctatgtt
      421 accttaaaac agtttgaaaa cccggcaaac ccggcaattc accaacaaac taccggtcct
      481 gaaatttggg atgcaactga aggtaaaatt gatgtattag tggcaggcgt aggaacaggt
      541 ggcactatta ccggtgtttc ccgtgctatt aagcaagata aaggcaagca aattatttca
      601 gtggcggtag aaccgagtga atcgccggtc attacgcaaa ctttaaacgg tgaagaagtc
      661 aaaccgggcc cgcacaaaat tcaaggcatt ggtgcaggct ttattccgaa aaacttagat
      721 ttatcactgc ttgaccgtgt tgaacaagtt tcaagcgaag aagcgattac aaccgcacgc
      781 cgtttaatgg cagaagaagg cattttaggc ggtatttcat caggtgctgc cgttgctgcc
      841 gcagatcgca tcgctaaact acctgaattt gccgataaat taattgtagt gattctgcct
      901 tcagcctcag agcgttattt aagtacgcta ctgtttgaag ggattgaagc gtaatattaa
      961 attaatataa aaatgccggc tgatgtcggc atttttattg
//
LOCUS       DANLXS010000321          979 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.329, whole genome shotgun sequence.
ACCESSION   DANLXS010000321 DANLXS010000000
VERSION     DANLXS010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.329"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            134..820
                     /locus_tag="PXD71_002683"
     CDS             134..820
                     /locus_tag="PXD71_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292739.1"
                     /translation="MAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGTITGT
                     TISGGTISGTTITGGIIKGARIEGLTGKFTGELEINQLIGGNIYEAKTWTSKATGKSK
                     TYSETKNSFALSDTYYEYVCQFVLPATKATRTLYVVHTPTGESKSETFYGSGSNRTPT
                     TITLPSVIDISNTISPNVSNPFNDSGAILIANTQYVVTCRAWCAGRGRSAKVNFRAII
                     SSTNGSSYQL"
BASE COUNT          344 a          178 c          202 g          255 t
ORIGIN      
        1 cccttggcaa aatgccggtg taaccgctat ccacggtggc tcaatagtta ctaataccgt
       61 tactgctcaa caaattgcag caaataccat taccgccaac gaaatcgcag ccggcacgat
      121 tgcagctcga aatatggcag caaactcgat taatgcaagt catattgtgg ggagttcgat
      181 cactgcggat aagctaaatg taaacaattt agcggcaata tcggcaaatc ttggtaaggt
      241 aactgctggt acaattacag ggacgacaat tagtggtggg acaatttcag gtacaacaat
      301 tacaggggga ataattaaag gagcaagaat tgagggatta acaggtaaat ttactggaga
      361 acttgaaatc aaccaattga tcggaggtaa tatctatgag gctaaaactt ggacatcaaa
      421 agcaacaggg aaaagtaaaa catattcaga aacgaaaaac agttttgcat taagtgatac
      481 atattatgag tatgtatgtc aatttgtact acctgctaca aaagcgacga gaacattgta
      541 tgttgtccac actccaacag gagagagtaa aagcgaaact ttttatggta gtggtagtaa
      601 tcggacaccg acaacaatta cattaccgtc tgtaattgat attagcaata ctatttcacc
      661 taatgtatct aacccattta atgattctgg ggcaatactc attgctaata ctcaatatgt
      721 agtaacgtgt cgtgcgtggt gtgcagggcg tgggagaagt gctaaagtga attttcgagc
      781 aattatatca tctacaaatg gttcatctta tcaattatag gaggcaaata tgaaaaaaat
      841 tatactactt acaacaatta tggtttcagc ttgtacaact caatataagc aagactatac
      901 aacaaaacca accttttgtt atcaactagc ccctcaagag caattaccgg ggaaaaactg
      961 tattggctca ggagggcat
//
LOCUS       DANLXS010000322          964 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.330, whole genome shotgun sequence.
ACCESSION   DANLXS010000322 DANLXS010000000
VERSION     DANLXS010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.330"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            223..964
                     /locus_tag="PXD71_002684"
                     /pseudo
     CDS             223..964
                     /locus_tag="PXD71_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
BASE COUNT          343 a          167 c          151 g          303 t
ORIGIN      
        1 gactcctcta atagtccact cttgttccat tttggaacat ttttgtatat tatttcgcct
       61 tttctttctc attgtgttct atgtaggcaa cagcattttt tgcaaagtga tcgacgagaa
      121 cattcattac ttcagtccat tttacaggtc ttcctatctt cattccaact tccatcgcta
      181 atctttctat cttgagtttt ttctctaatt taatattgaa cagagcttta tgaaatggtt
      241 gatatagaac aaacgtcagt tgattggatt gatgtaactt attcagctca tattgattcg
      301 gaatctatcc aaaaacaact tatctctttt ttccaaaaca tccatttagg acaaacacgt
      361 cgtacaagac ataacaaaga atatgacaca acggcagaat ggaattcagg ttcagaacac
      421 cgtattttaa aagtttatct aaaaggtaat gaagttcaaa aaagactaca agaagcccag
      481 cacgacttca aaaaatcacc aagtaaaaag tatctgcaaa actcaattca agtattaagt
      541 aatccaaatc tcattgaatt ttcaaaaaaa tgtgttcgtt ttgaagcaag attaaaacaa
      601 cgttatttag ataaacatca aatcccacgc aatttatttg acttaattaa atatcagaga
      661 gattacgaag agcagggtag aagcctgatt aaagacctat ggcaagacgc attcaaagac
      721 attattaagg ctgtcgggga aagcaatatg aatatttaca acagagatca aattcagcgt
      781 ttattacgga aaaattattt ttcaatcact ccaaaaggaa atatgagcta tgcaaaggct
      841 gatcgtttat tcagctttta taagaattta ctaagtgatg gctatagaga aacgctagat
      901 tctatgccaa gaaacacatt ttggagatat gaaaaggatc ttatggccgt tggtttaact
      961 aaaa
//
LOCUS       DANLXS010000323          957 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.331, whole genome shotgun sequence.
ACCESSION   DANLXS010000323 DANLXS010000000
VERSION     DANLXS010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.331"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>957
                     /locus_tag="PXD71_002685"
     CDS             <1..>957
                     /locus_tag="PXD71_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL6292740.1"
                     /translation="DKAGLLSEIVQKADQHSVTLAIGGDVNKLVQRPVNSLPKQIVLA
                     LRYLLEQKIKLNTPKGPADGWFTEDGLWLVSKTAADNIRTYLLGQGISVPKDNPKLFD
                     EMQSLGIVEESPSGGAIWSCRIQADSGWTPATTFTLLRVKPEVAWENINDRPAYFAGK
                     VKVIEQITEEVNAEVQPPLVEETTKISLLPSKQSEAIIPSEETEISKAITSSDNATSA
                     EVIINNENDLTSFVLDMFAPTETIEQNISVISSNKEQQIVMTTLTEQEKVPPKKVKLE
                     KSKKKNLQLTTLEPWNDMINPITGKEFVNWLKEGIATNKFAI"
BASE COUNT          327 a          173 c          214 g          243 t
ORIGIN      
        1 cgataaagcc gggcttttat ctgagatcgt gcagaaagcc gatcagcata gtgttacgct
       61 agcaatcggt ggcgatgtga ataagctggt acagcgacca gttaattcat tgccgaagca
      121 aattgtgctg gctctacgtt atttactgga gcagaaaatc aaattaaata cgccaaaagg
      181 accagcagac ggctggttta ccgaagatgg gctgtggctg gtaagtaaaa ctgctgccga
      241 taatatccga acttatttac tgggacaggg gatttccgtg cctaaagata atccaaaact
      301 gtttgatgaa atgcaatctc ttggtattgt ggaagagtct ccttccggcg gtgcaatttg
      361 gagctgccgt atccaagccg attccggttg gacaccggcg acaacattta cgctattacg
      421 agttaaaccg gaggttgctt gggagaatat caacgataga ccggcgtatt ttgccggaaa
      481 agtcaaagtg attgaacaga taactgaaga ggtcaatgca gaggttcagc ctccattagt
      541 tgaagaaacc actaaaattt ctctattacc gagcaagcaa tcggaggcta ttattccgtc
      601 tgaggaaacg gaaatatcga aggcaattac aagttcagac aatgcaacat ctgcggaagt
      661 aatcataaat aatgaaaatg atttaacttc ttttgtattg gatatgtttg ctcctacaga
      721 gacaattgag caaaatattt ctgtcatttc ttcaaataaa gagcagcaaa tcgtaatgac
      781 aacacttacg gaacaagaaa aagttccgcc gaaaaaagtt aaattagaga aaagtaagaa
      841 aaaaaatctt caattaacca ctctagaacc atggaatgat atgattaatc caattactgg
      901 aaaagaattc gtaaattggt tgaaggaggg gatagcaacc aataaatttg caattaa
//
LOCUS       DANLXS010000324          933 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.332, whole genome shotgun sequence.
ACCESSION   DANLXS010000324 DANLXS010000000
VERSION     DANLXS010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.332"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            86..>933
                     /gene="msrA"
                     /locus_tag="PXD71_002686"
     CDS             86..>933
                     /gene="msrA"
                     /locus_tag="PXD71_002686"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL6292741.1"
                     /translation="MKYNQLLLCLVSAIFTTSSIANITTPNEMRSENLQDIYLAGGCF
                     WGIEAYMEKIYGVKEATSGYANGKTEQTAYRIIGQTDHAETVHVTYDANKISLEKLLQ
                     YYFKVIDPTSKNKQGNDIGRQYRTGIYYQNEQDKQIILKEIQAQQAKYKQKIQVQVEP
                     LKHYILAEEYHQDYLKKNPNGYCHIDLNQANSIIIDPKDYPKPSDATLKMKLTPLQYS
                     VTQRKNTERSFSNEYWDNTAPGIYVDITTGEPLFSSLDKYDSGCGWPSFTKPIINEVV
                     NYTEDN"
BASE COUNT          359 a          168 c          159 g          247 t
ORIGIN      
        1 gcaaaaaagt aagaaattat gaatgtttaa ggttaaaccc tcatccttaa aaaataaaat
       61 tacataagtt ataaaggaga actggatgaa atacaatcaa ctgttactat gtttggtgtc
      121 tgctatcttt accacctcct caatagcaaa catcacaacc cctaacgaga tgcgatctga
      181 aaacttacag gatatttact tggcaggagg atgtttctgg ggcattgaag cctatatgga
      241 aaaaatatat ggcgtaaaag aggctacatc gggctatgcc aatggcaaaa ctgagcaaac
      301 tgcttaccga attatcgggc aaaccgatca tgcagaaaca gttcacgtga cctatgatgc
      361 taataaaatt tctttagaaa agctgttgca atattacttt aaagtaattg atccaaccag
      421 taaaaataaa caaggtaatg atattggccg ccaatatcgg acaggtattt attatcagaa
      481 tgagcaagat aaacaaatta ttttaaaaga aatacaagct caacaagcaa aatacaaaca
      541 aaaaatacaa gtgcaagtag aaccattaaa acattatatt ttggcagaag agtatcatca
      601 ggattaccta aagaaaaatc caaatggcta ctgccatatt gatttaaacc aagccaacag
      661 cattattatc gatccgaaag attatcctaa acctagtgat gcaacattaa aaatgaaatt
      721 aacgcctttg caatatagcg ttacccaacg taaaaatact gagcgttcat tcagcaatga
      781 atattgggat aataccgcac cgggcattta tgttgatata actacaggag agcctctttt
      841 ttcatctctc gataaatatg actccggctg tggctggcca agttttacca aaccaattat
      901 caatgaggtt gttaactata ccgaagataa tag
//
LOCUS       DANLXS010000325          923 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.333, whole genome shotgun sequence.
ACCESSION   DANLXS010000325 DANLXS010000000
VERSION     DANLXS010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.333"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            141..>923
                     /locus_tag="PXD71_002687"
     CDS             141..>923
                     /locus_tag="PXD71_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6292742.1"
                     /translation="MLNWAGKSTAYQTLQTPTSKTLTPCEEESVDFENTQNIFIEGEN
                     LDVLKALQKSYFNSVKMIYIDPPYNTGNDFIYNDSFADTKADYAEKVGDVDEHGKLKR
                     AFVRNSKENGHYHSNWLNMMLPRLHLAKNLLRDDGVIFISIDDNEQAQLKLLCDEVFG
                     EENFVAILSVENNPKGRKNSKFISVSNDFCIIYAKNKEMGKFVENIPKNAKDMCQDEN
                     GHYVHASGKRVLVGENKFNQPVTDFTSDKHYSVYFNKLDDDII"
BASE COUNT          321 a          153 c          172 g          277 t
ORIGIN      
        1 ttgccaactt ttcgcttaaa gatcaacaac tttctcaatt aaaatccctt ttacctgaaa
       61 ttttcaccga aggtaaaatc gacttcgaca aattccaaca acttttttcg gataattttt
      121 ccaccgaacc tgaccgctat atgctcaact gggcaggaaa atcaacagcc tatcaaacct
      181 tacaaacccc aaccagcaaa acgctcactc cttgcgagga agagagtgtg gattttgaaa
      241 atacgcagaa tatctttatt gagggtgaaa atttagatgt attaaaagcc ctgcaaaaat
      301 cctacttcaa ttcggtcaaa atgatttata tcgatccgcc atacaataca ggcaacgatt
      361 ttatttataa cgacagtttt gccgatacca aagcagatta tgcggagaaa gtgggcgacg
      421 ttgatgaaca cggcaaattg aaacgagcct ttgttcgtaa cagcaaagaa aatgggcatt
      481 atcacagtaa ctggctgaat atgatgttgc ctcgtttgca tctcgcaaaa aatttactgc
      541 gtgatgacgg ggtgattttt atttcaattg atgataacga acaggcacag ctgaaattgc
      601 tttgtgatga agtgtttggg gaggagaatt ttgttgccat attatcagtt gaaaataatc
      661 cgaaaggtag aaagaattct aaatttattt ctgtaagtaa tgatttttgt attatttatg
      721 ctaaaaataa agaaatgggg aaatttgttg aaaatattcc taaaaatgct aaggatatgt
      781 gtcaagatga aaatggacat tatgttcacg caagtggaaa acgagtatta gttggggaaa
      841 acaaatttaa tcagcctgta acagatttta cctcagataa acactattca gtttatttta
      901 ataaattaga tgatgatatt att
//
LOCUS       DANLXS010000326          922 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.334, whole genome shotgun sequence.
ACCESSION   DANLXS010000326 DANLXS010000000
VERSION     DANLXS010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.334"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            7..741
                     /locus_tag="PXD71_002688"
     CDS             7..741
                     /locus_tag="PXD71_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6292743.1"
                     /translation="MARSSYFARLAEWTLLEHLLVKQPENISVCSFGQSFGTSGRLDA
                     EYYQPKFASLFAQLANFKTMKLGEIVNLQKSVEPGSEAYQTEGIPFVRVSDLSKFGIS
                     QTDKYLHPKDFGNVVRPKKDSILLTKDGTVGIAYRVPQDLNVITSGAIVHLELKTDEV
                     LPDYLALALNSPAVQLQAERDAGGSIIQHWKPSEILDVVIPVLPKNIQQTISDKVQQS
                     FALRVESEVLLEKAKILVEQEIENMR"
BASE COUNT          321 a          150 c          180 g          271 t
ORIGIN      
        1 ggtttattgg cgagatcttc ttattttgca cgtttagcag aatggacttt attagaacat
       61 ttacttgtca aacagcctga aaatatttcg gtatgttcat tcggtcaatc attcggtaca
      121 agcggtcgtt tagatgcaga atattaccaa cctaaatttg caagcttatt tgcacaatta
      181 gccaatttca aaacgatgaa attaggtgag attgttaatt tacaaaaatc ggtagaaccg
      241 ggctcggaag cttatcaaac agagggaata ccctttgtgc gtgtatcgga tttatctaaa
      301 ttcggcatca gccaaacaga taaatactta catccgaaag attttggcaa tgttgtaaga
      361 cctaaaaaag acagtatatt actgactaaa gacggcacag tcggcattgc ttaccgtgtt
      421 ccgcaagatt tgaatgtgat tacatcaggg gcaattgtgc atctagagct taaaactgac
      481 gaagttttac ccgattattt ggcgttagca ttgaattctc ctgctgtaca attacaagcg
      541 gaaagagatg cgggaggctc tattatccaa cattggaaac cgtctgaaat tttagatgta
      601 gtgattccgg tattacctaa aaatatccag caaaccatta gcgacaaagt acaacaaagt
      661 tttgcattga gagtagaaag tgaagtttta ctggaaaaag ctaaaatact tgtagaacaa
      721 gaaatagaaa atatgaggta ataatgccta atcataatca agatttgaaa aaagagtggt
      781 ttaataaagc aaggatagat taccactctc cttttttaat gttatggctc agttgtaata
      841 gctggtataa cttccattat tccttaggta aggatagaac acatattgat agaataaaat
      901 cggataccag taatcaaaac aa
//
LOCUS       DANLXS010000327          859 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.335, whole genome shotgun sequence.
ACCESSION   DANLXS010000327 DANLXS010000000
VERSION     DANLXS010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.335"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>859
                     /locus_tag="PXD71_002689"
     CDS             <1..>859
                     /locus_tag="PXD71_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL6292744.1"
                     /translation="THTIKTETIAGGRKAIAGIAFGATADNRNAESSVIVMADKFEVV
                     KNAQDGTPVRLFGVVENKVAINGNLIATGSISGDHINANSVRTAVLTAGAIKTEHLAA
                     GQISADKLAIGLGGNLLVNPIFATPDLSIAPFGWNYWRGEVGDRLNIDTRTIFNRNEQ
                     DNYGLKNGGLPNELVFSMRYSTTKETSDTGRIGWLSQDINVVPNKKYIASVWLACHRG
                     QAKLVIENIEKPGGAYLGRIGNSEVITGYSAHQGEFKNMKRVKVVFTAPTSGCIRFAL
                     RLDNIQGKA"
BASE COUNT          264 a          151 c          212 g          232 t
ORIGIN      
        1 ctacccatac catcaaaacc gaaaccattg ccggtggacg caaagcgatt gccggtattg
       61 catttggtgc aacggcagat aaccgtaatg cggaatcttc tgtgattgtg atggcagata
      121 aatttgaggt cgtgaaaaac gcccaagacg gcacgccagt aagactattt ggcgtggtag
      181 aaaataaagt agcgattaat gggaatttaa tcgcaacagg ctcgatttca ggagaccaca
      241 tcaatgcaaa ttccgtcaga actgctgtat tgactgcagg agcgattaaa acggagcatt
      301 tggcagcagg gcaaatatcg gcggataagt tagcgattgg gctaggtggg aatttgctgg
      361 ttaatcctat ttttgcaaca cccgatttgt caatagctcc gtttggctgg aactactggc
      421 gaggagaagt tggtgatcgg ctaaatattg acacccgtac tattttcaat aggaatgaac
      481 aagataatta cggactaaaa aatggaggac taccaaatga gttagtattt tcgatgcggt
      541 atagcactac aaaggaaacc tctgatactg ggcgaattgg gtggttatct caggatatta
      601 atgttgtgcc taataaaaaa tatattgcct ccgtatggtt agcttgtcat cgtgggcaag
      661 ctaaattagt cattgagaat atcgaaaaac ctggtggtgc ttatttaggt cggataggca
      721 acagcgaggt aattactggc tatagtgctc atcaaggcga atttaaaaat atgaaacggg
      781 ttaaggttgt atttactgca cctacttcag gctgtattcg tttcgcatta cgattagata
      841 atattcaagg taaagctaa
//
LOCUS       DANLXS010000328          855 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.336, whole genome shotgun sequence.
ACCESSION   DANLXS010000328 DANLXS010000000
VERSION     DANLXS010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.336"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>855
                     /locus_tag="PXD71_002690"
     CDS             <1..>855
                     /locus_tag="PXD71_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292745.1"
                     /translation="ASIAQQNLQSIWRTDAQSAVDALKIGGANLLVDSEYLTTARWGG
                     SSRVASSQYGDRRLTQVFVTQAGTGHFGVTQGTQKATTRIRQGETYTLSLNAQGTAGF
                     TRTGLNYVYLIREDGGNFRLPTLPLTASLSQRPKVTFTAPWTSNQVRLLIGANGIFEA
                     TDWFAFHSVKLEMGNVATGWTPTAKDIDDKVSAVQSNLTAYQAAQAKADQAKATQISG
                     LTTRMGAAESNLTRTERAVTELNQTTVTTLRDLTARTKTTEGSLSRLETAKANKTEVA
                     SIAQSSLQS"
BASE COUNT          235 a          212 c          224 g          184 t
ORIGIN      
        1 gcaagtattg cccagcagaa cttacagagc atttggcgca ccgatgcgca aagtgcggtg
       61 gatgccttga aaatcggtgg tgcaaatcta ctggttgata gtgagtattt gacgacagcc
      121 cgttggggcg gtagctcacg agtggcaagc tcgcaatatg gtgatcgtcg cttgactcaa
      181 gtgtttgtga cccaagcagg cacagggcat tttggtgtga ctcaaggcac acaaaaagcc
      241 actacgcgta ttcgtcaggg tgaaacctat accttgtcac taaatgcgca ggggacggca
      301 ggttttaccc gcacagggtt aaactatgtg taccttatcc gcgaagatgg tggcaatttt
      361 agattgccaa ctttgccgct tactgcatca ttgtcccaac gccctaaagt gacatttact
      421 gcgccgtgga cgagcaatca agttcggtta ctgattggcg caaatggcat atttgaggcg
      481 acagactggt tcgcatttca cagcgttaag ctcgaaatgg gtaatgttgc cacaggctgg
      541 acaccaaccg ccaaggatat tgacgacaaa gtatcagcgg ttcagtccaa cctcaccgct
      601 tatcaggcgg cacaagctaa agcagatcaa gccaaagcta cgcagatttc ggggctcaca
      661 acccgaatgg gggcagcgga aagtaacctc actcgcaccg aacgggcagt gacggaactt
      721 aatcagacaa cggtgacgac actgcgtgat ctaaccgcgc gcaccaaaac aacggaaggt
      781 tcgctctctc gtttggaaac cgccaaagca aataaaactg aggtggcaag tattgctcaa
      841 agctccttac agtca
//
LOCUS       DANLXS010000329          842 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.337, whole genome shotgun sequence.
ACCESSION   DANLXS010000329 DANLXS010000000
VERSION     DANLXS010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.337"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>842
                     /locus_tag="PXD71_002691"
     CDS             <1..>842
                     /locus_tag="PXD71_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HDL6292746.1"
                     /translation="QFVIKLLEQLREQDSRFEFNPEVIRFLTYIAIASTDLYRFKDCN
                     SDNYPLLLDKAWEIHRFIFTQFSAYLNLSEIHDDTCRKDLLKMLIPIAMKMMLSISDD
                     VDLGLFNQTELHRNPILAHTVEKLVDDIEKNYGYSLSIREQYFIFDIFSGMVNRIELE
                     RKPLRLAIIALNGRLSTQQLKRNIKRYFSDYILKIETKVLYELAIMEDKPYDYYLCNE
                     YGKNMAIPYKPIYFADSSLNENEYATSLNEVFYHAYQYDEVLPSIKSIEIEAQYRLNE
                     FHLE"
BASE COUNT          276 a          139 c          150 g          277 t
ORIGIN      
        1 tcagtttgta attaagttac tggaacagct tcgtgaacaa gacagtcgtt ttgagtttaa
       61 tccggaagtt attcgatttc tgacctatat tgcgatagcc agtacagatc tctaccgctt
      121 taaagattgt aattcggata attacccatt gttgctagat aaggcttggg aaattcatcg
      181 ttttattttt actcagtttt ctgcatattt aaatttatcg gaaattcacg atgacacttg
      241 ccgtaaagat ttgcttaaaa tgctgatccc tatcgcaatg aaaatgatgc ttagcatttc
      301 agatgatgtg gatttagggc tatttaacca aacagaacta catagaaatc caattttggc
      361 tcatacagta gaaaaattag tggatgacat tgaaaaaaac tacgggtata gcctatctat
      421 tcgagaacag tattttattt ttgatatttt ttccggaatg gttaatcgaa ttgaattaga
      481 acgtaaaccg ctccgactgg cgattattgc ccttaatggc agattaagca cccaacagtt
      541 aaaacggaat attaagcggt acttttccga ctatattttg aaaattgaaa ccaaagtgct
      601 atatgagctt gccataatgg aagataaacc ttatgactac tatctctgta atgaatatgg
      661 aaaaaatatg gctattccct ataagcctat ttattttgct gacagtagtt taaatgaaaa
      721 tgaatatgca acatcactca acgaagtatt ttatcacgct tatcaatatg atgaggtgtt
      781 gcctagcatt aaatcgattg aaattgaagc acaatatcgc ctaaatgagt ttcatcttga
      841 gc
//
LOCUS       DANLXS010000330          841 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.338, whole genome shotgun sequence.
ACCESSION   DANLXS010000330 DANLXS010000000
VERSION     DANLXS010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.338"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            19..792
                     /locus_tag="PXD71_002692"
     CDS             19..792
                     /locus_tag="PXD71_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL6292747.1"
                     /translation="MNLSNALKLIKYNELDVLEVNHNKFKAKIALQGAQLLSWQPYNA
                     EQDVLWLSEIEPFRIGSAIRGGIPICYPWFGSIKEPAHGTARIQEWRLVEHTCSDENV
                     GLVFALENKAKMEMMLGETCELYFTHLDPEPAQLALHSYFNIGDIEKVEVQGLPTRCF
                     DKLTDQEVEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETVLWNPWH
                     NTVSAMTETAYQNMVCVETARINTLLQQNETLGVRVLVR"
BASE COUNT          278 a          134 c          187 g          242 t
ORIGIN      
        1 tcatcaaagg aataaattat gaacttgagt aatgcattaa aattaatcaa atataatgaa
       61 ttagacgtgc tagaagttaa tcacaataaa ttcaaagcta aaattgcatt acaaggagca
      121 caattgctaa gttggcagcc gtataatgca gagcaagatg tgctgtggtt aagtgagata
      181 gaaccgttta gaattggctc tgcgattcgt ggcggtatac caatttgtta tccgtggttc
      241 ggttcaataa aagagcctgc ccacggcaca gctcgcattc aagaatggcg tttagttgag
      301 catacttgtt ctgatgaaaa tgtcggtttg gtatttgcct tagagaataa agcgaaaatg
      361 gaaatgatgt taggcgaaac ttgtgagtta tattttaccc atttagatcc agaaccagca
      421 caactggcat tgcactctta ttttaatatt ggtgatattg aaaaagtgga agtgcaaggc
      481 ttgccaactc gctgttttga taagctaacc gatcaagaag ttgaagtgcc atcacctcgc
      541 aagataagtg aaaatgtgga ttgtatttat ccggcacaag cggtgaattt tattcaagat
      601 tttgcaaatc agcgcaccat taaagttgag catattaatg cgaccgaaac cgtactttgg
      661 aatccttggc ataataccgt tagtgcgatg acagaaacgg cgtatcaaaa tatggtatgt
      721 gtggaaaccg ctcggataaa tactctactt caacaaaatg aaacgctagg agtgagggtt
      781 ttagtgcgat agtgtttaat gagagcaagc agctgaatta gtaatattgt ttacaaattt
      841 a
//
LOCUS       DANLXS010000331          835 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.339, whole genome shotgun sequence.
ACCESSION   DANLXS010000331 DANLXS010000000
VERSION     DANLXS010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.339"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            7..678
                     /locus_tag="PXD71_002693"
     CDS             7..678
                     /locus_tag="PXD71_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HDL6292748.1"
                     /translation="MKKSTLAIILGGALLTGTAVAHQAGDVIFRAGAVHVKANSSSDA
                     KSKAATGIDVDLEVKNNTQLGLTATYMLADNVGIELLGATPFSHKINAKAKVLESGAE
                     VDLDRVVILKQLPPSLYAQYYFFELTAKVKPYVGAGLNYTRFYHAKSVSPAVTDLSVK
                     KHSFGPVANLGVDVKLTDNIYFNAAAWYTRIKTTAKFKAAGADHEVKVKLDPVVLFAG
                     FGVKF"
BASE COUNT          269 a          140 c          168 g          258 t
ORIGIN      
        1 atgaaaatga aaaaatcaac tttagcaatt attttaggtg gggcattatt aactggaact
       61 gcggtggcac atcaagcagg tgatgtgata ttccgtgccg gtgcggtaca tgttaaggca
      121 aattcaagtt cagatgcaaa atcaaaagca gcgacaggta ttgatgtaga tttagaagtg
      181 aaaaacaata ctcagttagg cttaactgca acttatatgt tagccgataa tgtgggtatt
      241 gagttattag gtgcaacccc tttctctcat aaaattaatg ccaaagccaa agttttagaa
      301 tcaggtgcag aagtagattt ggatagagtt gttatcttaa aacaattacc accgagcctt
      361 tatgcccaat actatttctt tgagctaact gcaaaagtaa aaccttacgt tggtgcaggt
      421 ttaaattaca ctcgcttcta ccacgctaaa tcagttagcc ctgctgttac tgacttaagt
      481 gtaaaaaaac attctttcgg accggtagca aacctaggtg ttgatgtaaa attaaccgat
      541 aacatctact tcaacgcggc agcttggtac acacgcatta aaacaaccgc aaaatttaaa
      601 gcagcaggtg cagatcacga agtaaaagta aaacttgatc cggttgtctt atttgccggt
      661 ttcggggtga agttctaatt atttgttggg aacgagttgg gctaatctta gtgattagtc
      721 cttttttgtt aaagttgttt gaatatagcc ctttccactt ttaattaaat aattgaagag
      781 ataataaaat ctcactatgc aaatatttat tggtgtaatt ggtaaatctt gcttg
//
LOCUS       DANLXS010000332          823 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.340, whole genome shotgun sequence.
ACCESSION   DANLXS010000332 DANLXS010000000
VERSION     DANLXS010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.340"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>823
                     /locus_tag="PXD71_002694"
     CDS             <1..>823
                     /locus_tag="PXD71_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292749.1"
                     /translation="FSYVTHYIKKHSYAYSKADNIQELLVKFFSRKQPVMQSAFNHQK
                     RIVKWLWAEFWDKNIISMTLRIDGYRNANLWHYLRYQSRIEVLYKIANEHPNLLPLLA
                     YVKPEYWKCSDLFSYQNWVRSRDKQPFILYAKCQDKMLCESKGQWRWLKKQSRIVVDQ
                     CFLHVNQWQLLTEIQMPFKIPVCVICFILTNFSMISCELDRSRQRRLLSIFIQYIYHY
                     WKDNGFRQLKIHLSHLRVLFQDIEDYMIHEGYLHGIPQTWQHLVYHLNLINSNTS"
BASE COUNT          282 a          123 c          142 g          276 t
ORIGIN      
        1 ccttttcata tgtaactcat tacattaaga aacattccta tgcctatagt aaggcagaca
       61 atattcaaga attattagta aagtttttct ctagaaagca acctgttatg caaagtgcat
      121 ttaatcatca aaaaaggatt gttaagtggt tatgggctga attttgggat aagaatatta
      181 tctcgatgac acttagaatt gatggttata gaaatgctaa cttatggcat tatttacgtt
      241 atcaatcacg gattgaggta ttgtataaaa tagcaaatga acacccgaat ctactaccat
      301 tattagccta tgttaaacct gagtactgga aatgctcgga cttatttagt tatcaaaatt
      361 gggtaagaag ccgtgataaa cagcctttta ttttgtatgc taaatgccaa gataagatgt
      421 tatgtgaatc aaaggggcaa tggagatggt taaaaaaaca gtcgagaata gttgtagatc
      481 aatgttttct acatgttaat caatggcagt tattaacaga aattcaaatg cctttcaaga
      541 tcccagtatg tgtcatatgt ttcatactta ctaattttag catgatcagc tgtgagttag
      601 atcgatctag acaacgtcga ttactctcta tttttattca atatatctat cattattgga
      661 aagataatgg gtttagacaa ttaaagatac atctttctca tctgagagtg ctttttcaag
      721 atattgaaga ttacatgata catgaggggt atttacatgg tatacctcag acttggcaac
      781 atttggttta ccacctcaac cttataaatt caaatacatc aaa
//
LOCUS       DANLXS010000333          802 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.341, whole genome shotgun sequence.
ACCESSION   DANLXS010000333 DANLXS010000000
VERSION     DANLXS010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.341"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..547
                     /locus_tag="PXD71_002695"
     CDS             <1..547
                     /locus_tag="PXD71_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL6292750.1"
                     /translation="IRALCTQISGLREALRDGVYKVRIGKQYLDPSALEKGLFYCLKK
                     GQTIHFTPVIKGAKSGGVFNFVLGAALIGAAFFTGGASIAAWGAGAKMMGMLGASMLL
                     GGVSQMLTKMPKAPTMGNETEKESSTAFSNLNNLVTQGKPVPLAYGLIRTGSLVISQG
                     VETITIKENQPASNKKTGFRK"
     gene            596..>802
                     /locus_tag="PXD71_002696"
     CDS             596..>802
                     /locus_tag="PXD71_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292751.1"
                     /translation="MNLTKAESNLNAKITEEKKARVDADKVNADKLTDITSRVANAES
                     TITNFQSTKANKSEVASLAQSSLQA"
BASE COUNT          261 a          158 c          188 g          195 t
ORIGIN      
        1 tatccgagcc ttatgtacac agattagcgg attgcgtgag gcattacgag acggtgttta
       61 taaagtccgt atcggcaaac agtatttaga cccgtcagcc cttgaaaaag ggctttttta
      121 ttgtctgaag aaagggcaaa ccattcattt tacccctgtt atcaaaggag cgaaaagcgg
      181 tggcgtgttt aattttgttt taggtgcagc cttgatcgga gccgcatttt ttactggcgg
      241 tgcttctatt gcggcttggg gagctggagc aaaaatgatg ggtatgttag gggcttcgat
      301 gttactagga ggagtttccc aaatgctcac caaaatgcca aaagccccca caatgggtaa
      361 tgaaacagaa aaagaaagct caacggcttt ttcaaattta aataacttgg tgactcaagg
      421 caaacctgtg ccgttggctt atggcttaat tcggaccggc tcgcttgtga tatcgcaggg
      481 agttgagacg atcaccatta aagaaaacca accggcaagc aacaagaaaa caggctttag
      541 aaaataaggg ataattaccg attcagcaca agcacagcaa cttaatcagt tatctgtgaa
      601 cttgaccaaa gcagagagca atttaaatgc caaaatcacg gaagaaaaaa aagctcgtgt
      661 tgatgcagat aaagtgaatg cagataaact cacagacata acaagccgtg ttgcaaatgc
      721 ggaatcaact attaccaatt tccaatcaac caaagcgaat aaaagtgagg ttgcgagcct
      781 tgcacaaagt tcgttgcagg ca
//
LOCUS       DANLXS010000334          801 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.342, whole genome shotgun sequence.
ACCESSION   DANLXS010000334 DANLXS010000000
VERSION     DANLXS010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.342"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>801
                     /locus_tag="PXD71_002697"
     CDS             <1..>801
                     /locus_tag="PXD71_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6292752.1"
                     /translation="KVEEFTETLKTLSRDDLSAEERTTKTIDSVKLRIYLNEQARNRK
                     NYINGFVPTDWIPEITDQMTKSKNLWLKYEEEAKTTLKPYQEETISRVIQDEINKKMA
                     EIGTTTLEIGGKEHELEDLENQINALALTDEEKLAEDVENDLISKLDEAKAALAKNQQ
                     TLKEKRDELLGLNNKLDSSPLVKKGKNALAEGTNAFASGENAIAFGTDSQATGNNAIA
                     LGANSKANAESAIAIGKGAQALKEKALALGENAIANRASAIAIGDNHS"
BASE COUNT          327 a          135 c          154 g          185 t
ORIGIN      
        1 agaaggtaga ggaatttact gaaacattaa aaactttatc tagagatgac ttaagtgcag
       61 aggaacgtac aaccaaaaca attgactccg ttaaattaag aatatatctc aatgaacaag
      121 cacgaaatcg taaaaattat attaatggat ttgtacctac cgattggatt cctgaaatta
      181 cagatcaaat gacaaaatcc aaaaatctat ggttaaaata tgaagaggaa gccaaaacaa
      241 cattaaaacc ataccaagaa gaaacaatat ctagggttat acaagatgaa attaataaaa
      301 aaatggctga aataggtact acaacactag agattggtgg taaagaacac gagctcgagg
      361 atcttgaaaa ccaaattaat gctttagctt taactgatga agaaaaactt gcggaagatg
      421 tggaaaatga tttaatctca aagcttgatg aggctaaagc agccttagca aaaaaccagc
      481 aaacgctcaa agaaaagcgt gatgaattac tagggctaaa taacaaatta gatagctcac
      541 cacttgttaa aaaaggtaaa aatgcacttg cagaaggcac gaatgccttt gcttctggtg
      601 aaaatgccat cgcattcggc acggattcac aagctacagg taataacgct atcgcactag
      661 gtgcaaattc aaaagcaaac gcagaaagtg cgattgcaat tggtaaaggt gcacaagcat
      721 taaaagaaaa agcccttgct ttaggcgaaa atgccattgc caacagagca tctgctattg
      781 cgattggtga taaccattca t
//
LOCUS       DANLXS010000335          791 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.343, whole genome shotgun sequence.
ACCESSION   DANLXS010000335 DANLXS010000000
VERSION     DANLXS010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.343"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>791)
                     /locus_tag="PXD71_002698"
     CDS             complement(<1..>791)
                     /locus_tag="PXD71_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292753.1"
                     /translation="GHYLCTKVKGVRFFNNIAIGGRNVSAYGATSCSDIFIFGNREQG
                     MAVTTVDVEAGVQVEDVQTGECVVANNIFRHDIWMSGAKNAIINSNLCRRLRVTSGNP
                     TDTGSNNVEFSNNQAKSIAISKYGSFDNPSLFIDNINFINNILEPSEFNQDVYIDGTI
                     LKQCQFINNRCISKSKQYSVRLARNNPFLNLTFKSNKLSDNPAIISGKQGVIFCDDLS
                     LGVNKHRLRLRFSSPINFTVNQEWQKMPFDRVLNDINGHVDIVSG"
BASE COUNT          253 a          162 c          125 g          251 t
ORIGIN      
        1 tgaccactga caatatcaac atgaccattg atatcattta acactctgtc aaacggcatt
       61 ttttgccatt cttgattaac agtaaaatta atcggcgatg aaaatcgcaa tcttaatctg
      121 tgcttattta ctcctaagct caaatcatcg cagaaaataa caccttgctt acctgatata
      181 atcgcaggat tgtccgataa tttattactc ttaaatgtta aatttaagaa cggattattg
      241 cgagcaagtc tgacagaata ttgcttagac ttgctaatac aacgattatt aataaattgg
      301 cactgtttta aaattgtccc gtcaatatac acatcttgat taaattcact gggctccaaa
      361 atgttattaa taaaattgat attatcaata aacaatgaag gattatcgaa agaaccatat
      421 ttcgatattg caatagattt tgcttgatta tttgaaaatt ctacattgtt gctgcctgtg
      481 tcagtcggat tacctgatgt gacacgcaag cgacggcaga ggttgctgtt gataatggca
      541 tttttagcac cagacatcca aatatcgtgt ctgaaaatat tattcgctac cacacattcg
      601 ccagtttgta catcttccac ttgtacgccc gcttccacat cgactgtcgt cactgccatc
      661 ccctgttcac gattaccaaa aatgaaaata tcagagcaag atgttgcacc gtaagctgat
      721 acattgcgac ctccaattgc tatattgtta aaaaatcgca cacctttaac tttggtgcag
      781 agataatgtc c
//
LOCUS       DANLXS010000336          769 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.344, whole genome shotgun sequence.
ACCESSION   DANLXS010000336 DANLXS010000000
VERSION     DANLXS010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.344"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..552
                     /locus_tag="PXD71_002699"
     CDS             <1..552
                     /locus_tag="PXD71_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6292754.1"
                     /translation="AGQERCIADEVPFEIPQNWTWVRLGEVGDWSSGATPNRHNPEYY
                     NGTIPWLKTGDLNNGIISSIPEFITDKALQECSVRLNPVGSVLIAMYGATIGKLGILK
                     IAATTNQACCACIPFNGIYNKFLFYYLMSQKAEFQKKSEGSGQPNISKEKIINYLFPL
                     PPIHEQHRIVQKIEQLFAEIEKL"
BASE COUNT          266 a          129 c          144 g          230 t
ORIGIN      
        1 gcaggtcagg aacggtgtat cgccgatgaa gtgccatttg agattcctca aaattggact
       61 tgggtgaggt tgggggaagt aggtgattgg agttcaggtg caactccaaa ccgacacaat
      121 cctgaatact ataatggtac gatcccgtgg ctaaaaacag gagacttaaa caacggaata
      181 atttcatcta tacctgaatt tattacagat aaagcattgc aggaatgttc tgtaagatta
      241 aatcctgttg gttcagtttt gatagcaatg tatggagcga caattggtaa gcttgggata
      301 ttaaaaatag cagcaacaac aaatcaggct tgctgtgcgt gtattccttt taatggaatt
      361 tataataaat tcttatttta ttatttgatg tcgcagaaag cagaatttca gaaaaaaagt
      421 gagggaagtg ggcaaccaaa tatatcaaag gaaaaaatta tcaattattt atttccttta
      481 ccaccaatcc acgaacaaca tcgcattgtt caaaaaattg aacaattatt tgcagagatt
      541 gaaaaattgt aggagtcatc atcgctttct tgggcgtgtt ggatacgccc tacagtcact
      601 aaattattga tcttttatca tatcggtttt caaccaacct aaaaggaaac ctatatgatc
      661 catctatttg aaaagcacct taatcaatct aatcttgctg aaaataccca atcagcttat
      721 ctttttgcgg taaaacaata tcacaaaaaa tttgatcagt tatctaaaa
//
LOCUS       DANLXS010000337          758 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.345, whole genome shotgun sequence.
ACCESSION   DANLXS010000337 DANLXS010000000
VERSION     DANLXS010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.345"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            31..>758
                     /locus_tag="PXD71_002700"
     CDS             31..>758
                     /locus_tag="PXD71_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6292755.1"
                     /translation="MWVRLGDICLKITDGTHHSPPNIDKSDFLYITAKNIKKDGLDLS
                     KISYVTKEIHNEIFSRCNPEKGDILYIKDGATTGVSIINTLNEPFSMLSSVALIKTSQ
                     EIDNEYLNYVMNSHYFYNISIGSMSGTGIPRITLTKLESYLVPVPPLLEQQRIVQKIE
                     ELLPLVERYEQTEQQLTKLNNTFPEQLKKSVLHAAIQGKLTEQDPNDELASCLIERIK
                     AEKNRLIAEKKLKKPKSVSEIVMR"
BASE COUNT          279 a          127 c          140 g          212 t
ORIGIN      
        1 gaaccacctt ttgagattcc tgagagttgg gtgtgggtga ggttggggga tatatgccta
       61 aaaattacag atggaactca tcattctccc ccaaatatcg ataagagtga ttttctttat
      121 attacagcta aaaatataaa gaaagatgga ttagatttat caaaaattag ctatgtaaca
      181 aaagaaattc ataatgaaat tttcagccgt tgtaatcctg aaaagggaga tattctatat
      241 attaaagatg gagccacaac tggagtaagt attataaata ctcttaatga gccatttagt
      301 atgttatcca gtgttgcctt aattaagacc tcacaagaaa tagataatga gtacttaaat
      361 tatgtaatga atagtcatta tttttataac atttctattg gaagtatgtc tggaacaggt
      421 attccaagaa taacattaac taaattagaa tcttatttag tcccagttcc gccactccta
      481 gaacaacagc gcattgtgca aaaaatcgag gaattattac cgcttgttga acgctacgag
      541 caaaccgaac agcaactcac taaactcaac aatacattcc ccgaacagct gaaaaaatcg
      601 gtgttacacg cagctattca gggtaaatta accgagcaag atccgaatga tgaactggca
      661 tcttgcctga ttgagcggat aaaagcggag aaaaatcgtc ttattgccga gaaaaaactc
      721 aaaaaaccaa aaagtgtttc agagattgta atgcgtga
//
LOCUS       DANLXS010000338          754 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.346, whole genome shotgun sequence.
ACCESSION   DANLXS010000338 DANLXS010000000
VERSION     DANLXS010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.346"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            82..741
                     /locus_tag="PXD71_002701"
     CDS             82..741
                     /locus_tag="PXD71_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDL6292756.1"
                     /translation="MTTLGSRIKAYREQLGISQYEVAERCTSIDNRDKNNKWGQSRIA
                     NYERNNRTPDLDDIEILSKVLGVSPETLAFDTNVQLARPTKSNSYPLISNIQAGLWTE
                     AFDFKDSEGYDYIDTEIDAGPDAFFLRISGMSMEPKFSEGDLVLIDIRKRPHPGDYVA
                     AVNGTGEATLKRYRELGEWSESGNPHFELIPLNPDFPTLSSMKQDIRIIGVAVEHRSY
                     L"
BASE COUNT          241 a          141 c          167 g          205 t
ORIGIN      
        1 actaattgta aatttcattt tgtgattcac aaggatataa cggagcgtgt taaaatgagg
       61 aaaatctaat aggagagtgc tatgacaacc ttaggtagta gaattaaggc ttacagagaa
      121 cagctaggaa tcagccaata tgaagttgct gaaagatgta catcaataga taatcgagat
      181 aaaaataaca aatgggggca atctcgcatt gctaattatg agagaaacaa tcgtactccc
      241 gatcttgatg atattgaaat actcagcaaa gtgcttggtg tttctccaga aacacttgct
      301 tttgatacaa acgttcagct tgccagaccg acaaaatcga actcatatcc gttgatcagc
      361 aatatacaag cgggtttatg gacagaagcc tttgatttca aagattcgga gggctacgac
      421 tatatcgata cggaaattga tgccggaccg gacgctttct ttttaagaat ttccggaatg
      481 tcgatggagc ctaaatttag tgaaggcgat ctggtgttaa tcgatattcg caaacgtcct
      541 cacccgggcg attatgtagc agcggttaat ggcacaggtg aggctacttt aaaacgttat
      601 cgggagttag gcgaatggtc tgaatccggc aatcctcatt ttgagctaat accgcttaac
      661 cccgactttc caacactcag ctcaatgaaa caagatattc gtattattgg tgtcgctgtt
      721 gagcatagaa gctatttgta aagtccacca aagc
//
LOCUS       DANLXS010000339          750 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.347, whole genome shotgun sequence.
ACCESSION   DANLXS010000339 DANLXS010000000
VERSION     DANLXS010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.347"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..350)
                     /locus_tag="PXD71_002702"
     CDS             complement(<1..350)
                     /locus_tag="PXD71_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292757.1"
                     /translation="MIQNDPRDAIILSLLANAVYGGHNNSQISYQETEEVNLYARLRQ
                     VEGDVREIKSNYLTKEAFYKAGGSTLIALVVTAGIALWTVYSHLDTKVEARFSKIDDK
                     FVQVETNIKNLDIRL"
     gene            complement(355..735)
                     /locus_tag="PXD71_002703"
     CDS             complement(355..735)
                     /locus_tag="PXD71_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292758.1"
                     /translation="MNNKRIKPEQFADFITQRTGRNLTCPICNSQEHYLHDGYDTFTE
                     LCGEKIIGVPTIPYRIEPSPDEVMKFAAPEFHSLHFNEREGTKYWLDQKARTAVIVTC
                     VCCNNMLFFDRERILEWIKGSQNE"
BASE COUNT          218 a          149 c          124 g          259 t
ORIGIN      
        1 aggcgaatat caagattttt gatattggtc tctacttgga caaacttatc atcaattttc
       61 gagaatcgag cttcaacttt ggtgtcaagg tgtgaatata ctgtccataa tgcaattcca
      121 gcagtaacta ctaatgcaat gagtgtagag ccaccagctt tataaaaagc ctctttcgtc
      181 aaataatttg acttgatttc tcttacatcg ccttcaactt gtcttaatcg agcatataaa
      241 ttcacttctt ccgtctcctg ataactaatt tgtgaattat tatgcccgcc gtatactgca
      301 tttgcaagta aggatagtat gattgcatct ctaggatcat tttgaatcat tggtctattc
      361 attttgtgag cccttaatcc attctaaaat tctctctctg tcgaaaaaaa gcatattatt
      421 acagcaaaca caagtgacaa taacggctgt tctcgctttt tggtctaacc aatattttgt
      481 tccttcccgt tcattgaaat gcaaagaatg aaattcagga gctgcaaatt tcataacttc
      541 atcaggagat ggctctattc ggtatggaat ggttggaaca ccgataattt tctctccgca
      601 taattccgta aaagtgtcat agccatcgtg taaataatgt tcttggctat tacaaatagg
      661 gcaagttaaa ttccgcccag tacgttgcgt gataaaatcc gcaaactgtt caggtttaat
      721 gcgtttatta ttcattttaa cttcctttct
//
LOCUS       DANLXS010000340          705 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.348, whole genome shotgun sequence.
ACCESSION   DANLXS010000340 DANLXS010000000
VERSION     DANLXS010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.348"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            150..>705
                     /locus_tag="PXD71_002704"
     CDS             150..>705
                     /locus_tag="PXD71_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292759.1"
                     /translation="MASALGAFLLLLVASSYADFYYTQTKQKEQLYLQAEAHQLLNYF
                     QQHFQHIGYQGSHRTDSNFDLFQQAGKSLNIVHKNCIIGFYDLNQDGCLGKRKTKTAA
                     CKLGTVNNTREVLKEIVGFKVENNEIYTFSSQLDNCVKEQCQTLLENCDGNWSKFTEA
                     NNFKTKKLDFSWRQEDNLLKIELEI"
BASE COUNT          268 a           98 c          132 g          207 t
ORIGIN      
        1 attagaatca aaatgtatta agctcagttt aaataaagaa aatgaaattt tgcaatttga
       61 acaattagga cgggtttatg tggcagcaaa aaataaacgt agcacctgca aagattagtt
      121 tatctgcttt tagcctaatt gaagtattaa tggctagtgc attaggggcg tttttattat
      181 tgcttgttgc ttcaagttat gccgatttct attacacaca aaccaaacaa aaagaacagc
      241 tctacttgca agcagaagct caccagttac taaactactt ccaacagcat tttcaacaca
      301 ttggctatca aggctctcac agaacagaca gtaattttga tttatttcag caagcaggga
      361 aaagcctgaa tattgtgcat aaaaattgca ttattggatt ttacgattta aaccaagatg
      421 gctgtttggg aaaacgtaag acaaaaacgg ccgcttgtaa gttaggaaca gtgaataata
      481 ccagagaggt gttaaaagaa attgttggct ttaaagtaga aaataatgaa atttatactt
      541 tttcatcgca attagataat tgtgtgaaag aacaatgcca aacattattg gaaaattgcg
      601 atggaaattg gagtaaattt accgaagcaa ataatttcaa aactaagaaa ctggatttta
      661 gttggagaca ggaagataac ttattaaaaa ttgagttaga aatag
//
LOCUS       DANLXS010000341          700 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.349, whole genome shotgun sequence.
ACCESSION   DANLXS010000341 DANLXS010000000
VERSION     DANLXS010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.349"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>700
                     /locus_tag="PXD71_002705"
     CDS             <1..>700
                     /locus_tag="PXD71_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6292760.1"
                     /translation="TDFPNINFSNEKYKDLIKGYSNIQGNLVTAIPNFLEKVNNDFAI
                     SARNRDLYSHYLPYQGIDDWENSNFNFRTLNGVYYDDNLTSTELLRKFMIQRDYNTLY
                     ALSSARTGNADSLAKNYHLVVAKMSYENLKKELSKKPDSTFNKLKKLYETNGEFLNEY
                     ILDNFRGNPLELLTVPALKESLENQESFPSRLYQKFNIFNQAIAQTTSNITKEEIDDL
                     EKTVENFNNFKNSID"
BASE COUNT          269 a          113 c          102 g          216 t
ORIGIN      
        1 actgatttcc ctaatattaa tttttcaaat gaaaaatata aagatctcat taaaggttat
       61 agcaatattc aaggtaactt agtaacagcc attcctaact tcctggagaa agttaataat
      121 gattttgcta taagtgctag aaatagagac ttatatagtc actaccttcc ttatcagggt
      181 attgatgatt gggagaattc taattttaac tttcgaacac taaatggagt ttattatgat
      241 gataatctaa cttcaacaga actcctgcga aaatttatga ttcaaagaga ttataacact
      301 ctttatgctc taagttctgc tcgcacagga aatgctgata gtctggctaa aaattaccat
      361 cttgtagttg cgaaaatgtc ctatgaaaac ctgaaaaaag aattatccaa gaaacccgac
      421 tctacattta ataaactgaa aaagctgtat gaaaccaatg gtgaattctt aaatgaatat
      481 attttagaca atttccgagg aaatccactt gaattattaa cagtacctgc gcttaaagaa
      541 agtttagaaa accaagagtc attccctagc aggctgtatc agaaatttaa tattttcaac
      601 caggctattg cacagaccac atcaaacatt actaaagaag aaattgatga tttagaaaaa
      661 actgtagaaa atttcaataa cttcaaaaat tcaattgatt
//
LOCUS       DANLXS010000342          691 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.350, whole genome shotgun sequence.
ACCESSION   DANLXS010000342 DANLXS010000000
VERSION     DANLXS010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.350"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>691)
                     /locus_tag="PXD71_002706"
     CDS             complement(<1..>691)
                     /locus_tag="PXD71_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6292761.1"
                     /translation="DNTTFKRSCVIQKRVIHALLMRELITRYGRKNIGFLWLFIEPLL
                     LTLVITVAWKYIKADKFSTLNIIAFMITGYPIAMMWRNASNRAQGAISANLSLLYHRN
                     VRVLDTIFTRVLLEVTGASIAQILLMTIFIAIGWIEMPNDIFYMLCAWILMAFFALGL
                     GLIICTLAHYIEPFGKIWGTFSFFLLPLSGAFFFVHNLPLTAQNIALWFPMIHGTEMF
                     RHGYFGETVITY"
BASE COUNT          258 a          125 c          114 g          194 t
ORIGIN      
        1 cataggtaat taccgtttca ccaaaataac catgtctaaa catttctgta ccatgaatca
       61 ttggaaacca taaggcaata ttttgagcag ttagcggtaa attatggaca aagaagaatg
      121 caccagaaag aggaagtaga aagaagctga atgttcccca aatcttacca aaaggttcaa
      181 tataatgtgc taaagtacag ataattaaac ctaaacctaa ggcaaaaaat gccattaata
      241 tccaagcaca gagcatatag aaaatatcgt taggcatttc tatccaacca atagcgataa
      301 atatagtcat taagagaatt tgggcgatag atgcgccagt aacttctaat aaaactcgag
      361 taaatatcgt atctagcact ctcacatttc tgtggtaaag caaacttaag tttgctgata
      421 ttgctccttg agctctatta gaggcatttc tccacatcat tgcgataggg tagcctgtaa
      481 tcataaaagc gataatattt aaagttgaaa acttatctgc ttttatatat ttccaagcaa
      541 cagtaatcac taacgttaac aataatggtt cgataaaaag ccataagaag ccaatatttt
      601 tacgcccata acgagtaatt aattccctca ttaaaagggc gtgaatcacc cgtttttgaa
      661 tcacacagga acgtttaaag gttgtattat c
//
LOCUS       DANLXS010000343          650 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.351, whole genome shotgun sequence.
ACCESSION   DANLXS010000343 DANLXS010000000
VERSION     DANLXS010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.351"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..260
                     /locus_tag="PXD71_002707"
     CDS             <1..260
                     /locus_tag="PXD71_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDL6292762.1"
                     /translation="KKLVLSILFVISPMALAYSCNDPIPYCKEMQSCEQAKFYLNQCN
                     AGRLDRDNDGIPCENVCGKKGKSEKKDDKKSDKSKSKKTSN"
     gene            292..>650
                     /locus_tag="PXD71_002708"
     CDS             292..>650
                     /locus_tag="PXD71_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDL6292763.1"
                     /translation="MMNNQSVWHLFCDESGISGKPFYAFGALWIREDKISLFEKEIQK
                     LREKHYCTDEIKWQGTNSKRFSEFYRELVSFFFQADYLFFNCIVVQQAIVNKAFHNGD
                     YEMAKQKHFNLLICNKI"
BASE COUNT          231 a           89 c          125 g          205 t
ORIGIN      
        1 tgaaaaaatt agtcctctcg attttatttg ttatttcgcc tatggctttg gcttatagct
       61 gcaatgaccc tatcccttac tgcaaagaaa tgcaatcttg cgagcaagca aagttttatt
      121 taaatcaatg caatgccggt cgattagata gagataacga tggcatacct tgtgaaaatg
      181 tttgtggcaa gaaaggaaag tccgagaaaa aagacgataa gaaatcagat aaatcaaaat
      241 ctaaaaaaac ttcaaattag agacaggcgt tgtgcctgat taaaaggggt aatgatgaat
      301 aaccagtcag tttggcactt attttgcgat gaatccggta taagcggtaa gcctttttat
      361 gctttcggtg cattatggat aagagaagat aaaattagct tatttgaaaa agaaatacag
      421 aaattacgag aaaagcacta ttgcactgat gaaataaaat ggcaaggtac aaattctaaa
      481 cgctttagtg aattttatcg tgaattggtt agttttttct ttcaggctga ttatttattt
      541 tttaactgta ttgtagttca acaagctatt gtcaataaag cttttcataa cggtgattat
      601 gaaatggcaa aacagaagca ttttaattta ttgatttgca acaaaattga
//
LOCUS       DANLXS010000344          644 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.352, whole genome shotgun sequence.
ACCESSION   DANLXS010000344 DANLXS010000000
VERSION     DANLXS010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.352"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>644
                     /locus_tag="PXD71_002709"
     CDS             <1..>644
                     /locus_tag="PXD71_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6292764.1"
                     /translation="EIEQTRAAYDRLKRSGVASQNELRRASEATKQRVRELNAELGKT
                     SFGDKAKGFGRGLMSAGVGIAAGAAMARQPAKNQMEFDRQLAMASNTAFSDRDVKGRI
                     EGKKELLAAVQEAVKVGGGTKEDALLTLDTLIASGTMKAETAMSLLPTLQKGAVATGA
                     SSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGNFELADMARWLPQQMAAG"
BASE COUNT          184 a          129 c          190 g          141 t
ORIGIN      
        1 gaaattgaac aaactcgtgc agcctatgac cgcttaaaac gctcaggtgt tgcctctcaa
       61 aatgagctac gccgtgcctc agaagccact aagcaacggg tgcgagagct gaatgcagaa
      121 ttgggtaaga caagctttgg ggataaggct aaaggatttg ggcgtggttt gatgagtgcc
      181 ggtgtcggta ttgcagcagg tgctgcaatg gctcgtcagc cagccaagaa ccaaatggag
      241 tttgaccgac agttagcgat ggcatcaaac actgcctttt ccgaccgtga tgttaaaggg
      301 cgtattgagg gcaagaaaga gttattagca gcggtgcaag aggcggttaa ggttgggggt
      361 ggaacaaaag aagatgctct tttaactctt gatacgctaa ttgcctcagg aacgatgaag
      421 gctgaaactg caatgagcct attaccaacc ttgcagaaag gtgctgtagc aacaggtgca
      481 agctccgagg atatggcaaa aattgcgatc tcctcaatgc aacaatttgg gattaaagaa
      541 gaggatattg ggcgggcatt ggatatggca gttgcagccg ggcaagcagg taactttgag
      601 cttgcagata tggctcgctg gctaccacaa caaatggccg cagg
//
LOCUS       DANLXS010000345          642 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.353, whole genome shotgun sequence.
ACCESSION   DANLXS010000345 DANLXS010000000
VERSION     DANLXS010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.353"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            26..>642
                     /locus_tag="PXD71_002710"
     CDS             26..>642
                     /locus_tag="PXD71_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6292765.1"
                     /translation="MGRPELERAVLSVQNQTYPCKHYVFVDGEKYHQQAADILKNFPS
                     VIVTYLPMNTGADGWVNSSINAIAPFLVKEDIICYLDDDNWFEPTHVESGVHALLEMQ
                     SDYAYSLRNFYDLDGEFLCIDTIESLGEYNCVYQEPLECNYQLGGVKGVLSINLNDCQ
                     HIDTNCYFIRSEIARLISTAWYTGIHNDRNVYKKLQELALKGNCT"
BASE COUNT          205 a           82 c          128 g          227 t
ORIGIN      
        1 aactgtagct gttatcagtt caacaattgg tcgtcctgaa ttagaaagag ctgttttaag
       61 cgtacaaaat cagacttatc cttgtaaaca ttatgttttt gttgatggag agaaatatca
      121 ccaacaagcg gctgatattt taaagaattt tccaagtgtt attgttactt atctcccaat
      181 gaataccggg gctgatggtt gggtaaatag cagcattaat gcaattgcac catttttagt
      241 gaaagaagat attatttgtt atctggatga tgataattgg tttgaaccta ctcacgttga
      301 atctggtgtg catgctttat tagaaatgca atctgattat gcttactctt tacgtaattt
      361 ttatgattta gatggcgagt ttttgtgtat tgatacgatt gaatcgttgg gagaatataa
      421 ttgcgtttat caagaaccat tagaatgtaa ttatcaatta ggtggtgtta aaggtgtttt
      481 atcaataaat ctgaatgatt gtcagcatat tgatacgaat tgctatttta ttcgtagtga
      541 gatagctcgc cttatcagta cggcttggta cacaggtatt cataatgata gaaatgtata
      601 taaaaaatta caagaattgg cactaaaagg aaattgtaca aa
//
LOCUS       DANLXS010000346          638 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.354, whole genome shotgun sequence.
ACCESSION   DANLXS010000346 DANLXS010000000
VERSION     DANLXS010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.354"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(19..303)
                     /locus_tag="PXD71_002711"
     CDS             complement(19..303)
                     /locus_tag="PXD71_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010128390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292766.1"
                     /translation="MANITLSHITPKHFFETVRLLKMQEHRIYIGVDSIEVRFKQKRF
                     ESKKETDKRLEQLEHFMLFLQEIINSDIPIDLDEFIYTYHRSVRRGFYRR"
     gene            complement(321..482)
                     /locus_tag="PXD71_002712"
     CDS             complement(321..482)
                     /locus_tag="PXD71_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL6292767.1"
                     /translation="MEKIENSSNLVLKLSLENQMLILESLEHPKEPNLVMLEALKKYS
                     LYVAKTSNK"
BASE COUNT          198 a          112 c          102 g          226 t
ORIGIN      
        1 cactcctctt tcacatcact aacgtcgata aaaacctctc cttactgacc tgtgataggt
       61 gtatataaac tcatctaaat ctataggaat atctgaatta atgatttctt gtagaaagag
      121 cataaaatgc tctaactgtt ctagtctctt atctgtttcc ttttttgact caaaacgctt
      181 ttgcttaaaa cgaacttcta tcgaatctac accgatgtaa attcgatgtt cctgcatttt
      241 gagtagtcgg acagtttcaa aaaaatgttt aggggtgatg tgagaaaggg ttatatttgc
      301 catagtttag cttgaaaacg ctatttatta cttgttttag caacatacaa agaatacttt
      361 tttaaagcct ctagcattac taaattaggc tctttaggat gttctaagct ctcaagaatt
      421 agcatttgat tttcaagaga aagctttaga acaagatttg aactattctc tattttttcc
      481 attttaatta tctcattaga tgactttcgc ataacaacag gttatgtgta aattctcgtg
      541 cagtgggaca agcccgattg tactgcactc gaattgtaac ataacctgta ggacattatg
      601 cgaaaattaa taatataagt ttttcatcaa tcggcggt
//
LOCUS       DANLXS010000347          630 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.355, whole genome shotgun sequence.
ACCESSION   DANLXS010000347 DANLXS010000000
VERSION     DANLXS010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.355"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
BASE COUNT          210 a          126 c           84 g          210 t
ORIGIN      
        1 catactttct ccattaaagt atttcaactt actttataca ttactttaat tgcatcttca
       61 gaaagtaaat cacaacgagg taatacttca ttgatagcct tccaagaaag taaatcttgc
      121 tactctatag cacctttcat taaaagtggt tgtttctcag aataactttc acgaaattct
      181 tgataagaaa ttggaaattg aattttcatt ttcatctcca ctagccttga taatctgtac
      241 catcaccaca atcgtagttt gagccagatt ggttattatt cgttctacct ggaactccaa
      301 acgtaggctt tattggtaaa gatgatggaa ttaattcttt tacaccgcta ggtaaagaat
      361 tttcatgtat tgtaataggt aggctcctcg ctaaatcaag ggctaaatta acagaagtac
      421 tggataaacc gcctaaagcc cccctcttgc aaaccctgta gcaaaagcaa cgccataaga
      481 tacaagatct ggttgattag ccttattttc agaattccct ccaactacta agcttaattg
      541 taaaattgat ggttctttca tttcattctc cctttaatct aattctgatc acaaaaacca
      601 atattaaaaa taattctaaa aataatacta
//
LOCUS       DANLXS010000348          604 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.356, whole genome shotgun sequence.
ACCESSION   DANLXS010000348 DANLXS010000000
VERSION     DANLXS010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.356"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..296
                     /locus_tag="PXD71_002713"
     CDS             <1..296
                     /locus_tag="PXD71_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292768.1"
                     /translation="DTRYNKNGQGAVSERNIIDAKNRFKASHPYAKIISCVRGSETYV
                     TRPAKANATTKSVKEKQSQPQESSSLSSMLLGAAVTAGVGFVANKWFSKKDNT"
     gene            324..>604
                     /locus_tag="PXD71_002714"
     CDS             324..>604
                     /locus_tag="PXD71_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292769.1"
                     /translation="MAGLLPPYFKKKNSDHYKDAVKQFGKEQVDEVIAQCDELESWVK
                     EMPTKQLIMYVGCFHTFNKLSQSKAYVQENFPNVLENPFLFQTLSAASH"
BASE COUNT          218 a          102 c          111 g          173 t
ORIGIN      
        1 cagataccag atataacaag aatgggcaag gtgctgtttc tgaaagaaat attatagatg
       61 ctaaaaatcg ttttaaagcg agccatccat acgcaaaaat tatttcttgt gttagagggt
      121 ctgagacata tgtaacacgt ccagcaaaag ctaatgctac aacaaaatct gtcaaggaaa
      181 agcaatctca accacaagaa agttcatcgc tcagttcaat gttacttggt gcggctgtaa
      241 ctgctggggt tggttttgta gccaataaat ggtttagcaa aaaagataac acttaattta
      301 tctctaacca atgtggagta aaaatggctg gattattacc accttatttc aaaaagaaaa
      361 atagtgatca ctataaagat gctgtaaaac aatttgggaa agaacaagtt gatgaggtta
      421 tagcacagtg tgatgaactt gaatcttggg taaaagaaat gccaacaaaa caacttatta
      481 tgtatgttgg ttgtttccat acttttaaca aattatctca atctaaagct tatgttcaag
      541 agaactttcc aaacgtactg gaaaacccgt ttcttttcca aacactttcg gcagcgtctc
      601 ataa
//
LOCUS       DANLXS010000349          581 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.357, whole genome shotgun sequence.
ACCESSION   DANLXS010000349 DANLXS010000000
VERSION     DANLXS010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.357"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..298
                     /locus_tag="PXD71_002715"
     CDS             <1..298
                     /locus_tag="PXD71_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6292770.1"
                     /translation="ACLIYLRKFGYSLGRPYADTVYQSKFPNMKELRIQNKGKPIRAF
                     YAFDPLRQAIVLCAGEKGKDKRFYEKMIALADAEFTSYLNNLEKENENVRSNAQ"
     gene            270..569
                     /locus_tag="PXD71_002716"
     CDS             270..569
                     /locus_tag="PXD71_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL6292771.1"
                     /translation="MKTLDQMLNELSPERRQKVEDMAQEMIREIQLSKLREELNLSQQ
                     QLATALGISQPAIAKLEQKDNDPRLSTLKRYIEAMGGKLKLAVELPTGDERVIRI"
BASE COUNT          201 a          107 c          111 g          162 t
ORIGIN      
        1 ggcttgttta atctatctgc ggaaattcgg ttatagtcta ggcagacctt atgctgatac
       61 agtttatcag tctaaattcc ctaatatgaa agagttacgc attcaaaaca aaggtaagcc
      121 aattcgagca ttttatgctt ttgacccttt acgccaagcg atagtacttt gtgctggcga
      181 gaaaggtaaa gacaaaagat tctatgaaaa aatgatcgct cttgctgatg cggaatttac
      241 ctcctattta aataatttag agaaagaaaa tgaaaacgtt agatcaaatg ctcaatgaac
      301 tctctcctga acgccgccaa aaagtggaag atatggctca agaaatgatt agagagatac
      361 aactttcaaa actaagagaa gagctaaatc tctctcaaca acaattagct acagctctcg
      421 gtatttcaca gcctgctatt gctaaacttg aacagaaaga taacgatcca agattatcca
      481 cgctcaaacg ctatattgaa gcaatgggcg gtaagttaaa acttgccgtt gaattgccta
      541 caggcgatga gcgtgtaatt aggatttaat tactttgtta a
//
LOCUS       DANLXS010000350          562 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.358, whole genome shotgun sequence.
ACCESSION   DANLXS010000350 DANLXS010000000
VERSION     DANLXS010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.358"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>562
                     /locus_tag="PXD71_002717"
     CDS             <1..>562
                     /locus_tag="PXD71_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDL6292772.1"
                     /translation="LVLLDVMMPVLSGVKTLKRIRQKYSLPVLMLSARDDQIDRILGL
                     ELGADDYVPKPFNNRELVARMKAILRRTTSRPASTDHSEQNYTENISKSLQFAGVELH
                     SGRQQAYYQGCDLDLTGTEFALLQMLMRNSGEILSRELLSLKILGKNLTPYDRAIDMH
                     ISNLRKKLPEREDGLPWFKTLRGRGYL"
BASE COUNT          191 a           88 c          112 g          171 t
ORIGIN      
        1 tttagtatta ctagatgtga tgatgccggt attaagtggc gtgaaaacat taaaacgtat
       61 acgacaaaaa tactcattac ccgttttaat gttaagtgca agagatgatc aaattgatag
      121 aatcttaggt ttagaattag gggctgacga ttatgtaccg aaacctttta ataatcgtga
      181 actagtcgct agaatgaaag cgatacttcg aagaacaacc tctcgcccag cttcaacaga
      241 ccattctgaa cagaattata ctgaaaacat ctctaaatca ttacaatttg ccggagttga
      301 attacattca ggacgtcagc aggcttatta tcaaggatgt gatcttgatt taacggggac
      361 tgaatttgca ttattacaaa tgttaatgcg aaactcaggg gagattttgt ccagagaatt
      421 attgagttta aaaattttag ggaaaaattt aacgccttat gatcgtgcaa ttgatatgca
      481 tatttctaat cttcgtaaaa aattacccga acgggaagat ggattacctt ggtttaaaac
      541 cttaagaggt cggggttact ta
//
LOCUS       DANLXS010000351          546 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.359, whole genome shotgun sequence.
ACCESSION   DANLXS010000351 DANLXS010000000
VERSION     DANLXS010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.359"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>546
                     /locus_tag="PXD71_002718"
     CDS             <1..>546
                     /locus_tag="PXD71_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL6292773.1"
                     /translation="PLAYEDYSLINQKFLKFQWKSNYAYANWLETTANKIKRSKDDSI
                     EWHRKNEKEIPFWVITETWDFGALSKFYSLLNGQHQRKIAKKFNVDENTLKNWLNEIN
                     IVRNLSAHHSRIWNRKFSDIAIPKLDDPRWSKFKKMQQYFSNLSLDDKAKERIFGRVA
                     ILWFLVSQTSTNYHWLDKFED"
BASE COUNT          193 a           72 c          103 g          178 t
ORIGIN      
        1 tcctttggct tatgaagatt attctctgat taatcaaaag ttcttaaaat ttcaatggaa
       61 atctaattat gcttatgcaa attggctaga aactacagct aataaaatta agcgtagtaa
      121 agatgattct attgaatggc atagaaaaaa tgagaaggaa atcccttttt gggtaattac
      181 tgaaacctgg gattttggtg cattatctaa gttttactca ttgttaaatg ggcaacacca
      241 aaggaaaatt gctaagaaat ttaatgtaga tgagaatacg ttaaagaatt ggttgaatga
      301 aataaacatt gtaagaaacc tttctgctca ccattctcgt atttggaaca gaaaatttag
      361 cgatattgct attcctaagc tagatgatcc tcgttggtcg aaatttaaaa aaatgcagca
      421 gtatttttca aacttatcgc tggatgacaa agcaaaggag agaattttcg gaagggtagc
      481 tattctgtgg tttttagtta gtcaaaccag cactaattac cattggttag ataagtttga
      541 agattt
//
LOCUS       DANLXS010000352          544 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.360, whole genome shotgun sequence.
ACCESSION   DANLXS010000352 DANLXS010000000
VERSION     DANLXS010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.360"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..229
                     /locus_tag="PXD71_002719"
     CDS             <1..229
                     /locus_tag="PXD71_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292774.1"
                     /translation="VANGIVNEPSVDCFSQEIAQFTGCHIFSSLYIAFNTQRSFKSHW
                     DSRDIFAVQMQGKKRWIIHTPTFKKTIVYAS"
     gene            219..>544
                     /locus_tag="PXD71_002720"
     CDS             219..>544
                     /locus_tag="PXD71_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HDL6292775.1"
                     /translation="MHRSKDMPEYAPNLDDVYMDIVLEAGDVLYLPRGWWHDPIPVGE
                     ETVHLAVGIFPAYTHNYLTWVSQNMVEKEIARASLSHYESDKELIAQLAEQTAEYIKD
                     KENYHK"
BASE COUNT          173 a           82 c          106 g          183 t
ORIGIN      
        1 agtcgcaaat ggtattgtaa atgaaccaag tgttgattgc ttctctcaag agatagctca
       61 atttacaggc tgtcatattt tttcaagttt atatattgcg tttaatactc aaagatcttt
      121 taaaagccat tgggatagtc gtgatatttt tgcagttcaa atgcagggta aaaagcgttg
      181 gattattcac actccaacat ttaaaaaaac cattgtttat gcatcgtagt aaagatatgc
      241 ctgaatatgc tcctaatctt gatgatgtat atatggatat tgtattagaa gctggagatg
      301 ttctttattt acctcgtggc tggtggcacg atcctattcc tgttggagaa gaaactgtgc
      361 atttagctgt aggcattttt cctgcttata ctcataacta tctgacttgg gtatctcaaa
      421 atatggttga gaaagaaatt gcccgagctt ctttatcgca ttatgagtca gataaagagt
      481 tgatagcaca gttggcagaa caaacagcag aatatattaa agataaagag aattatcata
      541 aatt
//
LOCUS       DANLXS010000353          543 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.361, whole genome shotgun sequence.
ACCESSION   DANLXS010000353 DANLXS010000000
VERSION     DANLXS010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.361"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..291
                     /locus_tag="PXD71_002721"
     CDS             <1..291
                     /locus_tag="PXD71_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292776.1"
                     /translation="TFHLKGKITVTNSSEDVLNLYQLRLLINNEEKERLINQSVSPKN
                     SKSNTNGMTTINFDTVVYLKVGDIVDLQYKTTHELTLLSDSFLSQIYVTQEF"
     gene            292..534
                     /locus_tag="PXD71_002722"
     CDS             292..534
                     /locus_tag="PXD71_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292777.1"
                     /translation="MTKKHYCTGWRSAPANINSCCHQHDRDYGIRGTVTRADADKRLR
                     ECMIKNGNPIRAWLFWLVVRAFGWRFYKNKEIKIEK"
BASE COUNT          196 a           82 c          107 g          158 t
ORIGIN      
        1 acttttcatt taaaaggtaa aataacggtt acaaattcat cggaagatgt gctgaattta
       61 tatcaactca ggctgttaat aaataacgaa gagaaagaaa gactgattaa tcaaagtgtt
      121 tcacctaaaa acagcaaatc aaatacaaat ggtatgacaa caatcaattt tgatactgta
      181 gtttatttaa aagtaggtga cattgttgat ttgcagtata aaacaacgca tgagttgaca
      241 ttactgtcag actcattttt atctcaaatt tatgtgactc aggagtttta agtgactaaa
      301 aaacactact gcacaggctg gcgtagcgca ccagccaata tcaacagttg ctgtcatcaa
      361 catgaccgag actacggcat tcgtggcaca gtaacacgag cagatgctga taaacgctta
      421 cgtgagtgta tgattaaaaa tggcaatcca attagggctt ggttattttg gctagttgtg
      481 agagcttttg gttggcgttt ttataaaaat aaggagatta agattgagaa ataaagataa
      541 tac
//
LOCUS       DANLXS010000354          543 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.362, whole genome shotgun sequence.
ACCESSION   DANLXS010000354 DANLXS010000000
VERSION     DANLXS010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.362"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>543)
                     /locus_tag="PXD71_002723"
     CDS             complement(<1..>543)
                     /locus_tag="PXD71_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292778.1"
                     /translation="FNESYDFRGKEFEEPVQAILVESDKIYFIGSTIDAIFDKTLIDN
                     TKASDVIYFLNKLLQSPYGRKISNHLITLAIYDENIQIQYSPQFIAKNLTMSDIQALQ
                     KLGFQFSHSKEYLWKSYAGLGGKITQFKNRKEILEKYKFKQPIIAKAKSYQKSIEFSF
                     DDTISVIAAPITIPASIILFV"
BASE COUNT          172 a           86 c           82 g          203 t
ORIGIN      
        1 tacaaataaa ataatagatg cgggaatcgt aattggtgct gcaatgaccg aaatggtgtc
       61 atcaaagcta aattctatgc ttttctgata acttttcgct ttagcgatta taggttgctt
      121 aaatttatac ttttctagta tttccttgcg atttttaaac tgtgtgattt taccaccaag
      181 acctgcatag cttttccata gatattcttt tgaatggcta aattgaaaac ctaacttttg
      241 tagagcttga atatcgctca tggtgagatt ttttgcaata aattgcggac tgtactgaat
      301 ttgaatattt tcatcataaa tggctaaagt aattaggtga ttactgatct tccttccata
      361 cggactttga agaagtttat tcaaaaaata aataacatct gaagcttttg tattatctat
      421 cagcgtttta tcaaatatcg catcaattgt ggaaccaatg aaataaattt tatctgattc
      481 aaccaaaatt gcttgtacag gttcttcaaa ttcttttcct ctaaaatcat aactttcgtt
      541 aaa
//
LOCUS       DANLXS010000355          532 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.363, whole genome shotgun sequence.
ACCESSION   DANLXS010000355 DANLXS010000000
VERSION     DANLXS010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.363"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>532
                     /locus_tag="PXD71_002724"
     CDS             <1..>532
                     /locus_tag="PXD71_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6292779.1"
                     /translation="KMPRGDKNAMKGFIFNIPSIEYQKKCIANYFAFDQKIQLNTQTN
                     QTLEAIAQAIFKSWFVDFDPVRAKAAALSEGKSEHEANLAAMSVICGKDTSELNDTEY
                     KALWQIAEAFPSEFGDEGLPIGWKFNQADNLFDVGIGKTPPRKESEWFSDNANDTEWI
                     SIKDMGNQGLFITESS"
BASE COUNT          188 a          105 c          113 g          126 t
ORIGIN      
        1 caaaaatgcc acgaggggat aaaaatgcaa tgaaaggttt tattttcaat atcccaagta
       61 ttgaatatca aaaaaaatgt attgctaatt attttgcctt tgaccaaaaa atccaactca
      121 acacccaaac caaccaaacc ctagaagcca ttgcacaggc aatcttcaaa agctggtttg
      181 tggattttga ccctgtgcgc gccaaagccg ccgccctaag cgaaggcaaa agcgaacacg
      241 aagccaacct tgcggcaatg tcggtgattt gtggtaaaga caccagcgag ttaaacgaca
      301 ccgaatacaa agcgctttgg caaatcgccg aagccttccc aagtgagttt ggagatgaag
      361 ggttacctat tggctggaaa ttcaatcaag cagacaattt atttgatgtt ggtattggga
      421 aaaccccacc aagaaaagag agtgaatggt tttctgataa tgcaaatgat acagaatgga
      481 tttctattaa agatatggga aatcagggat tatttatcac agaaagtagc ga
//
LOCUS       DANLXS010000356          496 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.364, whole genome shotgun sequence.
ACCESSION   DANLXS010000356 DANLXS010000000
VERSION     DANLXS010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.364"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>496)
                     /locus_tag="PXD71_002725"
     CDS             complement(<1..>496)
                     /locus_tag="PXD71_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292780.1"
                     /translation="KINDFPDVSNLLEVTINDEQWLVLERHDDFRELAKFGQKETYDR
                     QMWLQIRSYLVKESQFSKAKNWLAQQDFMERWMPEGNEHYGMNIHQFFAENTLNEWQE
                     IYRHRENKSPSFKVIPSVDSHTWESTYGNDGISFYAPIPMLYHGMKMKNAIKDGYWVD
                     LQNN"
BASE COUNT          156 a           95 c           61 g          184 t
ORIGIN      
        1 aaattatttt gtaaatcaac ccaataacca tctttaatcg catttttcat tttcattccg
       61 tgataaagca taggaattgg cgcataaaat gaaatgccgt cattgccata agtagattcc
      121 caagtgtgag aatcgacact aggtattacc ttaaatgaag gcgatttatt ttccctatgt
      181 cgataaattt cttgccactc atttaatgta ttttcggcaa aaaattgatg aatattcatt
      241 ccatagtgtt cattaccttc tggcatccat ctttccataa aatcttgttg tgccaaccaa
      301 ttttttgctt ttgaaaattg agattcttta actaaataac ttctaatttg taaccacatt
      361 tgcctatcat aagtttcttt ttgcccaaat tttgctaatt cacgaaaatc atcgtgtctt
      421 tctagaacaa gccactgttc atcattaatc gttacttcca aaagattact aacatcagga
      481 aaatcattta ttttta
//
LOCUS       DANLXS010000357          486 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.365, whole genome shotgun sequence.
ACCESSION   DANLXS010000357 DANLXS010000000
VERSION     DANLXS010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.365"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
BASE COUNT          162 a           68 c           88 g          168 t
ORIGIN      
        1 acttaaaact aagttaaatg aattttcctt aaatcaaaat ataggccgtt attttaagca
       61 agaagtaggg attacactcc actataaagg ggtatcaagt tcggtacatg ctggctttac
      121 aaaaggaaat acattacaag aacaaaaatt catttcactc aaagttggat atgagtggta
      181 agctttatgt aacctgaagc tacacttgaa tcggttcaaa cataatgtta ctaaaaaagc
      241 cttgaaacat tgattttcaa ggctttttag gtatcggttg aaccgtatag gattataatt
      301 tggtgctggg gtcatctgaa ttgtatcact tcaagcattg ataaataagg gttttggtta
      361 attcaaattt tatttgtact cctaaatata ctcctaaaat tcaaagtgcc ttattgctta
      421 ggctattttg tgtacaatta ttcggatcgt gtatagggaa atttagttcg tagttttcta
      481 tagtag
//
LOCUS       DANLXS010000358          469 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.366, whole genome shotgun sequence.
ACCESSION   DANLXS010000358 DANLXS010000000
VERSION     DANLXS010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.366"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(116..191)
                     /locus_tag="PXD71_002726"
     tRNA            complement(116..191)
                     /locus_tag="PXD71_002726"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Thr,seq:ggt)
     gene            complement(195..269)
                     /locus_tag="PXD71_002727"
     tRNA            complement(195..269)
                     /locus_tag="PXD71_002727"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Gly,seq:tcc)
     gene            complement(313..397)
                     /locus_tag="PXD71_002728"
     tRNA            complement(313..397)
                     /locus_tag="PXD71_002728"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Tyr,seq:gta)
BASE COUNT          145 a          108 c          108 g          108 t
ORIGIN      
        1 tttctaaaag cctcttaaac aaaaacggtt acaatggaac aaaagaaccc attaaccata
       61 atgtagaaat taataccaat aaaaatttgc gtgagaaaga tagaattcag agaagtggtg
      121 ctgataggcg gatttgaacc gccgacctca cccttaccaa gggtgcgctc taccaactga
      181 gctatatcag cgcttggagc gggcagcggg aatcgaaccc gcatcattag cttggaaggc
      241 taaggtaata gccattatac gatgcccgct gttctaaatt cgtctgacct acgcgattca
      301 aaatatcaga gatggtggag ggagaaggat tcgaaccttc gaaggctgag ccaacagatt
      361 tacagtctgc cccctttggc cgctcgggaa cccctccacg ataattgaat acttatttac
      421 gcctgagtta atggtgccga ctaccggaat cgaactggtg acctactga
//
LOCUS       DANLXS010000359          461 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.367, whole genome shotgun sequence.
ACCESSION   DANLXS010000359 DANLXS010000000
VERSION     DANLXS010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.367"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
BASE COUNT          164 a           86 c           74 g          137 t
ORIGIN      
        1 catcaggtaa attttctaac ttccatcttc gtttttctaa aatcgaaaag taatactggt
       61 catcgtctaa aatctcaata tatgtacttc caaagagaac ttttgtataa ggcgtatcat
      121 gatagatcgt gacatcagcc ttcaatttgt actcgccttc catgcattcc ccaaaatggt
      181 attgactaaa aacaatggtt atgatataat gacatggggc ttgaaaagca agctgattgc
      241 tcaataggta atcagaccac tttgaaagct cggtcctttt caaaagaaaa ggtcttacta
      301 attactcaca aaatctggag aacaatatga aaaacactac agcgcaacaa aagccaatga
      361 aagttacagc taaacatatt agagaatcta ctactcgttg ttaagagctt agctttgata
      421 taaaaagacc aactttttat atcggcgtag taggtctaat t
//
LOCUS       DANLXS010000360          456 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.368, whole genome shotgun sequence.
ACCESSION   DANLXS010000360 DANLXS010000000
VERSION     DANLXS010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.368"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..439)
                     /gene="ssb"
                     /locus_tag="PXD71_002729"
     CDS             complement(<1..439)
                     /gene="ssb"
                     /locus_tag="PXD71_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL6292781.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GERREVTEWHRIVFYRRYAEICGQYLRKGSKIYIEGKLRTRKWQDQNGQERYTTEIQG
                     ELLQMLDSRNATNSVDGQQSNYQAMSQSPKATHPQDLPMDNFDD"
BASE COUNT          114 a          101 c           80 g          161 t
ORIGIN      
        1 catcatcaaa gttatccatt ggtaaatctt gtggatgtgt tgcttttgga gattggctca
       61 tagcttgata gttagattgt tgtccatcta cgctatttgt agcattacgg ctatctaaca
      121 tttgtaatag ttcaccttgg atttcggttg tataacgctc ttgcccgttt tggtcttgcc
      181 acttacgtgt ccgtaatttt ccttcgatat aaattttaga gcctttacgt aggtactgac
      241 cacaaatttc agcataacgg cgataaaaaa cgatacgatg ccattcagtg acttctcgac
      301 gttctccggt atttttatcc gtccaacttt cagaagttgc cacactaata ttagcaactg
      361 cttcaccatt aggcatagtc cgcatttcag gatcgttgcc aagattaccg acaataatca
      421 ctttattgat tcctgccatc gtatttactc cttcaa
//
LOCUS       DANLXS010000361          448 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.369, whole genome shotgun sequence.
ACCESSION   DANLXS010000361 DANLXS010000000
VERSION     DANLXS010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.369"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            29..268
                     /locus_tag="PXD71_002730"
                     /pseudo
     CDS             29..268
                     /locus_tag="PXD71_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
BASE COUNT          155 a           62 c           97 g          134 t
ORIGIN      
        1 tttagttttt ttaattaagg agccttatat gaaattcaaa aaattagcat tagcagcaat
       61 gttaggtggc ttatttgtaa cagctaatgc ggcagaatta cctaatatca ctattttagc
      121 gacaggcgga acgattgctg gtagtcagca gaatgcaaat agtagtgctt atcaagcagg
      181 gcaattgaat atcgaaacct taattgaagc agtgccagaa atgaaaacat tggcgaatat
      241 caaaggtgag cagattgtga aaattggccc actaaagcct tgttagatgt aggctatcaa
      301 ggtattgtcg tagcaggtgt tggtaacggc aatatgagtg cggaaaatct tgcattattt
      361 agaggaagcc aacaatattt ttaagatttt taataaacgt taaagcatat aaaaaatggc
      421 ataagtgagt cacttatgcc attatttt
//
LOCUS       DANLXS010000362          437 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.370, whole genome shotgun sequence.
ACCESSION   DANLXS010000362 DANLXS010000000
VERSION     DANLXS010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.370"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>437)
                     /locus_tag="PXD71_002731"
     CDS             complement(<1..>437)
                     /locus_tag="PXD71_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha2 family transposase"
                     /protein_id="HDL6292782.1"
                     /translation="QHKLIELFVAGVTARTAAELVNVNKTTAAYYFHRLRLLIYQHSP
                     HLEMFEGEIEADESYFGGARKGKRGCGAAGKTAVFGLLKRDDKVYTVVVPNTQSATLL
                     PIIREKVKPDSLVYADFYRSYDVLDVSEFNHFRINHSTHFAEN"
BASE COUNT          122 a          102 c           92 g          121 t
ORIGIN      
        1 tgattttcag cgaagtgtgt gctgtgattg atacgaaaat ggttaaattc actcacatca
       61 agcacatcat agcttcgata aaaatcggcg taaacaaggc tgtctggctt cactttctcc
      121 cgaataatcg gcaaaagtgt ggcagattgc gtatttggta cgacaacggt ataaaccttg
      181 tcatcacgtt ttaacagccc aaatacggca gttttgcctg cggcaccaca ccctcgttta
      241 cctttgcgag caccaccgaa ataactttcg tccgcttcaa tttcaccctc aaacatttcc
      301 agatgagggc tatgttgata gatgagcaat cgcaaacggt gaaagtaata ggcagcggtg
      361 gttttgttta cattcaccaa ctcagccgct gttcgagcag tgacacctgc tacaaacagt
      421 tcaatgagtt tatgttg
//
LOCUS       DANLXS010000363          430 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.371, whole genome shotgun sequence.
ACCESSION   DANLXS010000363 DANLXS010000000
VERSION     DANLXS010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.371"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>430)
                     /locus_tag="PXD71_002732"
     CDS             complement(<1..>430)
                     /locus_tag="PXD71_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6292783.1"
                     /translation="DLLNLIKEGDNRVVLHFNERNKGLIYSLNKAINLSSGEYIARMD
                     ADDISLPTRLSEQILYLEENHLDLCGCNALLFSEHGNLGLTNKLNSHEALEKLMVAGD
                     IGIIHPTFLARRKVFVESGGYNPLALHAEDMEFIAHILSMG"
BASE COUNT          153 a           87 c           49 g          141 t
ORIGIN      
        1 cccattgata aaatatgggc aataaattcc atatcttccg catgtaaagc taagggatta
       61 taccctccag attcaacaaa tacttttctc cttgctaaaa aagtagggtg aattattcca
      121 atatcaccag ccaccataag cttttctaaa gcctcatgac tattcaactt atttgtaaga
      181 ccaaggttgc catgttcaga aaaaagcaaa gcattacaac cgcataagtc taaatggttt
      241 tcttctaaat ataaaatttg ctctgacaat ctagtaggta aagatatatc atcagcatcc
      301 attctcgcta tatattcacc agaggataag tttatagctt tatttaatga atatatcaac
      361 cccttatttc tttcattaaa atgtaaaaca actctattat ctccctcctt tattaaattt
      421 aataaatcaa
//
LOCUS       DANLXS010000364          428 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.372, whole genome shotgun sequence.
ACCESSION   DANLXS010000364 DANLXS010000000
VERSION     DANLXS010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.372"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>428
                     /locus_tag="PXD71_002733"
     CDS             <1..>428
                     /locus_tag="PXD71_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL6292784.1"
                     /translation="FVSAGGVRSIWLRHHLANFKQRLIALEKLVAEQGIILSETQVQA
                     LERKKEDEIACGEIETVHPGYLGSQDTFYVGNLKGVGRIYQQTFIDTYSKVAFAKLYT
                     MKTAISAADMLNDKVLPYFESQGLPMLRILTDRGSEYCGKV"
BASE COUNT          133 a           74 c          100 g          121 t
ORIGIN      
        1 tttgtttcag caggaggtgt acgttcgatt tggttacgtc atcatcttgc caattttaag
       61 caaagattaa tcgccctgga aaaactggtt gcagaacaag gtattatact cagtgaaaca
      121 caggtacaag ccttagagcg taagaaagaa gatgagattg cctgtggtga aattgaaacg
      181 gtacaccctg gctatcttgg ctcacaagat actttctatg tgggcaacct caaaggagtg
      241 ggtcgtattt atcaacaaac gtttattgat acttacagta aagtggcgtt tgcaaaactt
      301 tatacaatga agaccgctat cagtgctgcg gatatgctga atgataaagt gttaccatac
      361 tttgaaagcc aaggtttacc gatgttacgc atattaactg accgaggaag tgaatattgt
      421 ggcaaggt
//
LOCUS       DANLXS010000365          413 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.373, whole genome shotgun sequence.
ACCESSION   DANLXS010000365 DANLXS010000000
VERSION     DANLXS010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.373"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>413
                     /locus_tag="PXD71_002734"
     CDS             <1..>413
                     /locus_tag="PXD71_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL6292785.1"
                     /translation="GVRSIWLRHNLANFKQRLNALEKAVAEKGIILSENQVQALERKK
                     EDDVACGEIETAHPGYLGSQDTFYVGNLKGVGRIYQQTFIDTYSKVAFAKLYTMKTAI
                     SAADMLNDKVLPFFESQGLPMLRILTDRGSEYCGKV"
BASE COUNT          122 a           76 c          102 g          113 t
ORIGIN      
        1 ggtgtgcgtt ctatttggct acgccataat ctcgctaact ttaaacagcg tttaaatgcg
       61 ttagaaaaag cagtggctga gaaaggcatt attctgagtg aaaaccaagt tcaagccttg
      121 gagcgtaaaa aagaagacga tgtggcttgt ggcgaaattg aaacggctca tccgggctat
      181 ctaggttcac aagatacctt ctatgtgggg aatcttaagg gggttggacg catttatcaa
      241 cagaccttta ttgatactta tagtaaagtc gcatttgcaa aactgtatac gatgaagaca
      301 gcaattagtg cagcagatat gctcaatgat aaagtcctac cgttctttga gtcccaagga
      361 ttaccgatgt tgcgtattct taccgaccga ggcagtgagt actgtggcaa ggt
//
LOCUS       DANLXS010000366          366 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.374, whole genome shotgun sequence.
ACCESSION   DANLXS010000366 DANLXS010000000
VERSION     DANLXS010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.374"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>366
                     /locus_tag="PXD71_002735"
     CDS             <1..>366
                     /locus_tag="PXD71_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292786.1"
                     /translation="NTTNVDLKITIGTRQYSHSFNGKYSSQYLESVVFDNLPPVPFNI
                     SVERLTEDSNSQRLQNGTIWSSYTEIIDTEFTYPNSAVAGISFDSEYFNNIPTRNYLI
                     KAKKVKVPSNYDPVKRTYTG"
BASE COUNT          117 a           84 c           67 g           98 t
ORIGIN      
        1 aataccacca atgttgattt aaaaatcacg attggcacac ggcaatattc gcatagcttt
       61 aacggcaaat acagctcgca ataccttgaa tctgtggtgt ttgataacct gccgcctgtg
      121 ccatttaata tctcagttga gcgtctaacc gaggatagca attcgcaacg cttacaaaac
      181 ggtacaattt ggagtagtta caccgaaatt atcgataccg agtttaccta ccctaattca
      241 gcggttgccg gtatcagttt tgattcggag tattttaata atatcccgac ccgaaactac
      301 ctaattaaag cgaaaaaagt caaagtgcca agcaattatg atccagttaa acgcacttac
      361 accggc
//
LOCUS       DANLXS010000367          358 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.375, whole genome shotgun sequence.
ACCESSION   DANLXS010000367 DANLXS010000000
VERSION     DANLXS010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.375"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
BASE COUNT          115 a           57 c           60 g          126 t
ORIGIN      
        1 gtcttgtgtt taatgagagg gttgttagaa taaaacatga gagtttcctt tttgtttaga
       61 ttgatttttg acactcatat tctaaacggg aaactctcac tttttagagt gaattgtcag
      121 atcaagtctg atcttctaca cctgatatta atttgcctga tctggaagtt cctacggcgg
      181 aatcaattat atcccctttt aaaaaattgc taccagattt acagaatttt gaactgaata
      241 aacgaaccgc acaatgccct gtttggacat ttaatattga atacttgaga tttcattatg
      301 aacttagaga acattgcgat ttgatagaac cacataaaga aatcttaaag ctattatt
//
LOCUS       DANLXS010000368          357 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.376, whole genome shotgun sequence.
ACCESSION   DANLXS010000368 DANLXS010000000
VERSION     DANLXS010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.376"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            10..>357
                     /locus_tag="PXD71_002736"
     CDS             10..>357
                     /locus_tag="PXD71_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL6292787.1"
                     /translation="MLLFLKNHNIATVEQLVKVTEASPATIRRDLIKLDEEGVVIRTH
                     GGVSLNQFIPFQPTTNEKQFKQIVEKERIAEYAVSLISPGNSILLDAGTTTLCIAKKI
                     AHMPLRVITTDLHI"
BASE COUNT          123 a           75 c           63 g           96 t
ORIGIN      
        1 ctagaacaaa ttttactatt cctaaaaaac cacaatattg ctaccgtaga gcaactggta
       61 aaagtgactg aggcttctcc tgcaactatc cgccgtgatt taataaaatt agatgaagaa
      121 ggcgtcgtga ttcgcacaca cggaggggtc agcttaaacc aatttatacc gtttcaacca
      181 acaacaaatg agaagcaatt taagcaaatt gtcgaaaaag aacgcatagc tgaatatgcc
      241 gtttcgctta tttcaccggg aaattctatt ttattagatg ctggcactac aacgctatgc
      301 attgcgaaaa aaatcgctca tatgccactt cgagtgatta caactgatct acatatt
//
LOCUS       DANLXS010000369          354 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.377, whole genome shotgun sequence.
ACCESSION   DANLXS010000369 DANLXS010000000
VERSION     DANLXS010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.377"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            31..107
                     /locus_tag="PXD71_002737"
     tRNA            31..107
                     /locus_tag="PXD71_002737"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:65..67,aa:Ile,seq:gat)
     gene            164..239
                     /locus_tag="PXD71_002738"
     tRNA            164..239
                     /locus_tag="PXD71_002738"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ala,seq:tgc)
BASE COUNT          107 a           75 c           90 g           82 t
ORIGIN      
        1 aagaagacaa aaaaccgaag gtcatccgtt gggtctgtag ctcaggtggt tagagcgcac
       61 ccctgataag ggtgaggtcg gtggttcaag tccactcaga cccaccactc agaaggcaag
      121 cgaaagcgaa gcgactatga gtgaagaaaa cggaagatga aatggggata tagctcagct
      181 gggagagcgc ctgccttgca cgcaggaggt cagcggttcg atcccgctta tctccaccaa
      241 tcatcgtgac tttatcatca tacctaagat tacaagcggt taaaaatacc ggattatttg
      301 caattttagg tatgatgata ggcgaaagcc attatctttg ttctttaaca atat
//
LOCUS       DANLXS010000370          354 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.378, whole genome shotgun sequence.
ACCESSION   DANLXS010000370 DANLXS010000000
VERSION     DANLXS010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.378"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(69..145)
                     /locus_tag="PXD71_002739"
     tRNA            complement(69..145)
                     /locus_tag="PXD71_002739"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(109..111),aa:Arg,seq:acg)
     gene            complement(159..253)
                     /locus_tag="PXD71_002740"
     tRNA            complement(159..253)
                     /locus_tag="PXD71_002740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:Ser,seq:gct)
BASE COUNT          101 a           74 c           75 g          104 t
ORIGIN      
        1 tatttgaatt gtaagtttat ttaattttat cagcaaccat tttaatttga atagagtaaa
       61 aattaaaatg gcgcacccga gaggattcga acctctgacc gctcggttcg tagccgagta
      121 ctctatccag ctgagctacg ggtgcgtgat aaagtaaatg gcggtgagag agggattcga
      181 accctcgata gagcttttta accctatact cccttagcag gggagcgcct tcagcctgct
      241 cggccatctc accactcact gtgtctgtgg gcgccatatt actagaattt aaaaagatgt
      301 caaattcttt ttcctgaaaa ttgaaaaatc ccgagtgttt gcctattgaa taaa
//
LOCUS       DANLXS010000371          353 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.379, whole genome shotgun sequence.
ACCESSION   DANLXS010000371 DANLXS010000000
VERSION     DANLXS010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.379"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>353
                     /locus_tag="PXD71_002741"
     CDS             <1..>353
                     /locus_tag="PXD71_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292788.1"
                     /translation="EYKFISPFEQTDRNEWKHEDIEFIYDEKISTEPPPYMEEEKFQK
                     IIMRFSKDIKIMMHNHINFIESDRFAEWILEKACVRSKTLTSFYGDKSVIRAVPPLDW
                     SGKYKYTKIGFRLCY"
BASE COUNT          141 a           49 c           62 g          101 t
ORIGIN      
        1 atgaatataa gtttatttct ccgtttgaac aaacagatag aaatgaatgg aagcatgaag
       61 atatagaatt tatatatgat gagaaaatat ctacagaacc acctccttat atggaggaag
      121 aaaaatttca aaaaataata atgaggtttt caaaagatat aaaaataatg atgcacaacc
      181 acataaactt catagaatct gacagatttg cggaatggat tcttgaaaag gcttgtgttc
      241 gctctaaaac attaaccagc ttttatggcg acaagtctgt tataagggcc gttccaccat
      301 tggattggtc aggaaaatat aaatatacca aaataggatt tagattatgc tac
//
LOCUS       DANLXS010000372          346 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.380, whole genome shotgun sequence.
ACCESSION   DANLXS010000372 DANLXS010000000
VERSION     DANLXS010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.380"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..333
                     /locus_tag="PXD71_002742"
     CDS             <1..333
                     /locus_tag="PXD71_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292789.1"
                     /translation="AIEYQSKKWKSTAVLFSRVPALVVFSISFFIFTAFILFIVFGNY
                     TRRETVVGELVMQSHPILLSAPKSGYISENYIQPHQQVKKRAIRWLKLRWIELRIAKI
                     LMLILFFR"
BASE COUNT          105 a           63 c           58 g          120 t
ORIGIN      
        1 gctattgaat atcaatcgaa aaaatggaaa agcacagctg tactatttag cagagttcca
       61 gctttggttg tcttttccat ttctttcttt atctttactg cctttattct atttattgtg
      121 tttggtaatt atacccgccg tgaaacggtt gtgggtgaat tagtgatgca atctcatccg
      181 attttattat ctgccccaaa atccggctat atatccgaaa attatattca accacatcag
      241 caggtaaaaa aaagggcgat ccgctggtta aaattacgct ggatagaatt acgcatagcg
      301 aaaatattaa tgttaattct attctttcgt tgaaatcaca aattca
//
LOCUS       DANLXS010000373          338 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.381, whole genome shotgun sequence.
ACCESSION   DANLXS010000373 DANLXS010000000
VERSION     DANLXS010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.381"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>338
                     /locus_tag="PXD71_002743"
     CDS             <1..>338
                     /locus_tag="PXD71_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6292790.1"
                     /translation="AVDSLSIGARNLLIDSTYNQHINYNTKMSSITRTVYRGNMLIRL
                     GMLGGAGVAGIVQAPAAQVSNIRQGQEYTLSLNVQGTAGIQKTGLNYVFLMRADGANQ
                     RLATIPVIAS"
BASE COUNT          104 a           79 c           76 g           79 t
ORIGIN      
        1 ctgccgttga ttcactttct atcggtgcaa ggaacttact gatagacagt acttacaacc
       61 aacacatcaa ctacaataca aaaatgtcat caatcacacg cacagtctat cgcggcaata
      121 tgttgatacg actcggaatg cttggtggcg caggtgtggc cggtattgta caagcaccag
      181 ccgctcaagt aagtaatatc agacagggtc aagagtacac acttagcctg aatgtacagg
      241 gtacagccgg catacaaaaa acggggctca actatgtatt cttgatgcgt gctgatgggg
      301 ctaatcaacg gttagcaaca atacctgtta tagccagc
//
LOCUS       DANLXS010000374          335 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.denovo.382, whole genome shotgun sequence.
ACCESSION   DANLXS010000374 DANLXS010000000
VERSION     DANLXS010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.denovo.382"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
BASE COUNT          108 a           69 c           49 g          109 t
ORIGIN      
        1 accttatcta tcaatacttg aagccattaa gttcaagtct cgaccaggca ccatttgact
       61 aaatttagtt caacagagct aaacgaaaaa accttagaaa gcctgtaaaa tcaacgttta
      121 caggcttttt tgttgtttta gctcaatggc actaaacatt tcatatcgca aattttaagt
      181 agcctagcaa attttccgcc aaatatgacc gcttgtaatg ccgttaggtt attgcctggc
      241 ggcctttttg ttacagctct tatatttttt acaccaaagt tcagaaaaag tcacttttat
      301 cactaacatt aagatgaaac cttgatacaa agctt
//
LOCUS       DANLXS010000375         6956 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.guided.129, whole genome shotgun sequence.
ACCESSION   DANLXS010000375 DANLXS010000000
VERSION     DANLXS010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.guided.129"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..218)
                     /locus_tag="PXD71_002744"
     CDS             complement(<1..218)
                     /locus_tag="PXD71_002744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292791.1"
                     /translation="MPENSDWISCSGCENSRSSGKKPNFDPKPQKTRNDSSYNLFIWG
                     RAPVALATLNDSSYNSDEPATPAQRRAS"
     gene            complement(381..1673)
                     /locus_tag="PXD71_002745"
     CDS             complement(381..1673)
                     /locus_tag="PXD71_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL6292792.1"
                     /translation="MGTYIPHREGIIQTIVRNSLTQYQAYASPSQTILDRLEHFAECA
                     DWTKGVVRIRCHDCGHSYFRPFSCKVFHLCPSCDQKRTLLYAEYLAEDLLLDLPHRQF
                     VFTIPKILRPYFKSDKRLFGEVSRLVFSLLSDFFSLAAGQELLCACVVSYQSFGEFAR
                     FHPHWHVLVLEGGFTKYDRFVYLPIGADEGMLKVWQAAIMALFLRKELIDQARVNMLR
                     DWKHSGFSIESETRLFNKADREALGQYVVRGATCAEKIQYDPASDTIIWTASPKGFYK
                     GKTETFKGFEFVDQLVAHLPPRRVQLLRRYGVYAGKVRKQWQERPNIYSLAPESWQKG
                     HPRESKIVPAIPQEKPETVQVSDAWSKLRKQSWARLLQKVYEVDPFVCPKCQGTMSVV
                     AIIEDPKELAKIINWAKQQEREQPVAVCARSPPELALV"
     gene            1836..2681
                     /gene="sul2"
                     /locus_tag="PXD71_002746"
     CDS             1836..2681
                     /gene="sul2"
                     /locus_tag="PXD71_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDL6292793.1"
                     /translation="MEYTSKSQIARNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQA
                     RKLMAEGADVIDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQ
                     AYALSRGVAYLNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHI
                     AAFFDARIAALTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLL
                     SVSRKSFLRALTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKE
                     TARIR"
     gene            2850..3653
                     /locus_tag="PXD71_002747"
                     /note="aph(3'')-Ib"
     CDS             2850..3653
                     /locus_tag="PXD71_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDL6292794.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3653..4489
                     /locus_tag="PXD71_002748"
     CDS             3653..4489
                     /locus_tag="PXD71_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDL6292795.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            4825..5640
                     /locus_tag="PXD71_002749"
     CDS             4825..5640
                     /locus_tag="PXD71_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDL6292796.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(5794..6387)
                     /locus_tag="PXD71_002750"
                     /pseudo
     CDS             complement(5794..6387)
                     /locus_tag="PXD71_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
     gene            complement(6388..6537)
                     /locus_tag="PXD71_002751"
                     /pseudo
     CDS             complement(6388..6537)
                     /locus_tag="PXD71_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
     gene            6609..6794
                     /locus_tag="PXD71_002752"
     CDS             6609..6794
                     /locus_tag="PXD71_002752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292797.1"
                     /translation="MNEQNNSWVQEWLNKSAKASERMATDEKFRQEVAKRATNSGQDI
                     QAILAKNIAKYQNQAKD"
BASE COUNT         1655 a         1792 c         1814 g         1695 t
ORIGIN      
        1 atcgacgctc gccgttgtgc tggtgttgcc ggttcatcag aattatagga actgtcattt
       61 aaagtcgcta aggctacagg tgcacgcccc caaataaata aattatagga actgtcattt
      121 cgggtttttt gcggtttcgg gtcaaaattc ggttttttcc ccgaactacg actgttttcg
      181 caccccgaac aactaatcca atcagaattt tccggcaacg ccgactccaa gaagtgtaat
      241 tccagtctat ttctgccgtc aacccctgat tcgcgttaat gcgacaaagc tcaaacacat
      301 aactccccca cgaagccata taaactccga tttcgaggtg ttatcctgtt ccgcataaac
      361 ttcgtttttg ggtattatac ctacaccaaa gcaagctcag gaggtgaacg agcacagacg
      421 gccactggct gctcccgttc ctgctgcttt gcccagttaa taatcttggc aagctccttg
      481 ggatcctcga ttatcgccac aaccgacatt gttccctggc attttgggca aacaaacggg
      541 tcaacttcat agactttctg taacaaccgt gcccaacttt gtttgcgcaa cttggaccag
      601 gcatcggaaa cttggacagt ttccggtttc tcctgaggta tggctggaac aattttggat
      661 tcgcgtggat gacctttttg ccaactttct ggggccaggc tataaatatt ggggcgctct
      721 tgccactgct tgcggacttt gcccgcatat acgccatagc gacgaagcaa ttgaacccgc
      781 cttggtggta aatgggccac aagctggtcg acgaattcaa agcctttgaa ggtctctgtt
      841 tttcctttgt agaatccctt tgggctagct gtccagatta tcgtgtcgga agctggatca
      901 tactgaattt tttctgcaca ggtagcgccg cgaacgacat actggccgag ggcttctcga
      961 tcggccttgt tgaatagtct ggtttcgctc tcgatgctaa agccggaatg cttccagtct
     1021 ctgagcatgt tcactcgggc ttgatctatt agttcctttc gcagaaacaa agccataatc
     1081 gctgcttgcc agaccttgag catcccttcg tcggctccaa ttggtaaata tacaaagcgg
     1141 tcgtacttgg tgaacccacc ttccagaacc agaacatgcc agtgcgggtg gaatcgagcg
     1201 aattcaccaa acgactgata gctgacaaca caggcacata gtagttcttg accggctgcc
     1261 agggagaaaa aatcagaaag caaggagaat accagcctgg acacctcgcc aaacagtcgc
     1321 ttatccgatt taaagtatgg tctgagaatc ttgggaatcg taaaaacaaa ttgccgatgc
     1381 ggtagatcaa ggagcaaatc ctcggccagg tattcagcat agagtaaggt ccgtttttgg
     1441 tcacaggatg ggcaaaggtg gaaaaccttg cacgaaaatg gcctgaagta cgaatgtcca
     1501 caatcatggc aacgtattcg aacaacaccc ttggtccaat cggcacattc agcaaaatgc
     1561 tcaaggcggt caaggatagt ttgcgatgga cttgcgtagg cttggtactg ggttagagaa
     1621 ttgcggacta tggtctggat tatcccttcc cgatggggaa tgtaagttcc catacctata
     1681 catacgcagg aaaacgatca tttgcttgca attgcgaaga aaataatgga aaatgtggac
     1741 tggcgccacc taacaaccgg ttcgagcgga ctcacgggac aagcccgttc gcagctcaac
     1801 cgaaagttag gttgacgcct tcggcgcgga ggaaaatgga atatacaagt aaatcacaaa
     1861 tagctcgaaa taaatcgctc atcattttcg gcatcgtcaa cataacctcg gacagtttct
     1921 ccgatggagg ccggtatctg gcgccagacg cagccattgc gcaggcgcgt aagctgatgg
     1981 ccgagggggc agatgtgatc gacctcggtc cggcatccag caatcccgac gccgcgcctg
     2041 tttcgtccga cacagaaatc gcgcgtatcg cgccggtgct ggacgcgctc aaggcagatg
     2101 gcattcccgt ctcgctcgac agttatcaac ccgcgacgca agcctatgcc ttgtcgcgtg
     2161 gtgtggccta tctcaatgat atccgcggtt ttccagacgc tgcgttctat ccgcaattgg
     2221 cgaaatcatc tgccaaactc gtcgttatgc attcggtgca agacgggcag gcagatcggc
     2281 gcgaggcacc cgctggcgac atcatggatc acattgcggc gttctttgac gcgcgcatcg
     2341 cggcgctgac gggtgccggt atcaaacgca accgccttgt ccttgatccc ggcatggggt
     2401 tttttctggg ggctgctccc gaaacctcgc tctcggtgct ggcgcggttc gatgaattgc
     2461 ggctgcgctt cgatttgccg gtgcttctgt ctgtttcgcg caaatccttt ctgcgcgcgc
     2521 tcacaggccg tggtccgggg gatgtcgggg ccgcgacact cgctgcagag cttgccgccg
     2581 ccgcaggtgg agctgacttc atccgcacac acgagccgcg ccccttgcgc gacgggctgg
     2641 cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt cgggatattt
     2701 ctctatattc gcggttcagc aggcatgtcc cctttgaggg cgacccgacg acaggataat
     2761 cgaccttatg gtgcgcaaat atttcggcac agatggtatt cgtggcaaag ccacctccgc
     2821 gcttcatcag aaaactgaag gaacctccat tgaatcgaac taatattttt tttggtgaat
     2881 cgcattctga ctggttgcct gtcagaggcg gagaatccgg tgattttgtt tttcgacgtg
     2941 gtgacgggca tgccttcgcg aaaatcgcac ctgcttcccg ccgcggtgag ctcgctggag
     3001 agcgtgaccg cctcatttgg ctcaaaggtc gaggtgtggc ttgccccgag gtgatcaact
     3061 ggcaggagga acaggagggt gcatgcttgg tgataacggc aattccggga gtaccggcgg
     3121 ctgatctgtc tggagcggat ttgctcaaag cgtggccgtc aatggggcag caacttggcg
     3181 ctgttcacag cctatcggtt gatcaatgtc cgtttgagcg caggctgtcg cgaatgttcg
     3241 gacgcgccgt tgatgtggtg tcccgcaatg ccgtcaatcc cgacttctta ccggacgagg
     3301 acaagagtac gccgcagctc gatcttttgg ctcgtgtcga acgagagcta ccggtgcggc
     3361 tcgaccaaga gcgcaccgat atggttgttt gccatggtga tccctgcatg ccgaacttca
     3421 tggtggaccc taaaactctt caatgcacgg gtctgatcga ccttgggcgg ctcggaacag
     3481 cagatcgcta tgccgatttg gcactcatga ttgctaacgc cgaagagaac tgggcagcgc
     3541 cagatgaagc agagcgcgcc ttcgctgtcc tattcaatgt attggggatc gaagcccccg
     3601 accgcgaacg ccttgccttc tatctgcgat tggaccctct gacttggggt tgatgttcat
     3661 gccgcctgtt tttcctgctc attggcacgt ttcgcaacct gttctcattg cggacacctt
     3721 ttccagcctc gtttggaaag tttcattgcc agacgggact cctgcaatcg tcaagggatt
     3781 gaaacctata gaagacattg ctgatgaact gcgcggggcc gactatctgg tatggcgcaa
     3841 tgggagggga gcagtccggt tgctcggtcg tgagaacaat ctgatgttgc tcgaatatgc
     3901 cggggagcga atgctctctc acatcgttgc cgagcacggc gactaccagg cgaccgaaat
     3961 tgcagcggaa ctgatggcga agctgtatgc cgcatctgag gaacccctgc cttctgccct
     4021 tctcccgatc cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg atcaaaacgc
     4081 aggttgtcaa actgactacg tccacgcggc gattatagcc gatcaaatga tgagcaatgc
     4141 ctcggaactg cgtgggctac atggcgatct gcatcatgaa aacatcatgt tctccagtcg
     4201 cggctggctg gtgatagatc ccgtcggtct ggtcggtgaa gtgggctttg gcgccgccaa
     4261 tatgttctac gatccggctg acagagacga cctttgtctc gatcctagac gcattgcaca
     4321 gatggcggac gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc tcgaccaggc
     4381 gtacgcttat gggtgccttt ccgcagcttg gaacgcggat ggagaagagg agcaacgcga
     4441 tctagctatc gcggccgcga tcaagcaggt gcgacagacg tcatactaga tatcaagcga
     4501 cttatcctat cccctgggaa cacatcaatc ttaccggaga atatcgttgg ccaaagcctt
     4561 agcgtaggat ttcgccctct cccgcaaacg acccctttat tgtaggtggg ccagttggtg
     4621 attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga
     4681 tccttcaact cagcaaaagt tcgatttatt caacaaatcc acgttgtgtc tcaaaatctc
     4741 ttatgttata ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     4801 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg
     4861 ccgcgattaa attccaacct ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     4921 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg
     4981 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     5041 aactggctga cggcatttat gcctcttccg accatcaagc attttatccg tactcctgat
     5101 gatgcatggt tactcaccac tgcgctcccc gggaaaacag cattccaggt attagaagaa
     5161 tatcctgatt caggtgaaaa tattgttgat gcgctggcag cgttcctgcg ccggttgcat
     5221 tcgattcctg ttagtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     5281 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     5341 tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca
     5401 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     5461 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     5521 tggaactgcc tcggtgaatt ttctccttca ttacagaaac ggctttttca aaaatatggt
     5581 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctga
     5641 tagttagtct ttgtagagac acaaacctga aattccggac accgcgcttc aggacatctc
     5701 cctggacgcc gatatctggc agtatccgga cggtactatc gaacgccggg ccaacggtac
     5761 tcccctgccc tttatgactg acgaccggct atttcaccca cgcacttctc catgaaattt
     5821 agcatcgtcg cggttaatgt cgaaaatttg ccacctgacg tggttggcat cgagggtgaa
     5881 aactgccgtg gcgagggtgt tctcctcatc cggatcgtcg gcagccagcc ggtaaattgg
     5941 cagttctgcg tcatgggtat ctgagagaat ggtttttgcc gccgcggcgt caaacggagg
     6001 ttgcgtttcc cgccactgca cgaggcgtaa ctgccgcgac gctgaactgt cggtaacgat
     6061 ctgctcaacg ccagccagtg ccgcatgcac cagatggttg gcatgcccgg ttaccgtcgt
     6121 caggggtaga acggaacagc cttgaccggt ggcctcaacg ctgaacagcc gcgcatcgcc
     6181 cagctgcccg agcgtatggt gaaacgcccc ggatcgcggc tggccggtaa gtatagtgat
     6241 ggcctcgtcg agggtgttcg cattcagcga ggcgcgagcc aggatctgac gcggtaatcc
     6301 ctgcggacga tacactgcac ggatgttatt caccgtgtta acgatacctt tttcatttac
     6361 cgcaaaggtg tggccgcaaa tcgacccctt gggtggcagt ccgaacagcg cccgatcatg
     6421 gtcgcgctct aggtagctat ccaacccatc gagcaggcgc tgttctggcc actcgccgct
     6481 ttcaaccttg ccgagtatcc acgcgcctac cagcaccttg cgccgggtgt cctccttggc
     6541 gtataacgag caagttttgg cttaccaaac cttaaaatcc acacaatcac agacacagga
     6601 aaaatcagat gaacgagcaa aacaactctt gggttcaaga gtggttgaac aagtcagcga
     6661 aagcgagcga gagaatggca acggacgaga aatttcgtca ggaagtcgcc aaacgagcaa
     6721 cgaacagcgg acaggatatt caggcaattc tagccaagaa tatcgccaaa tatcaaaatc
     6781 aggcgaaaga ttaactaata aaacgtctga aaacgctctc atagagccta caagacctat
     6841 caaaaataaa aatggtagta aggtagcacc cacaccaaca aacacgccta aaacgcaaaa
     6901 taacgcaaat tctaatctat tgtcatcaga acagtatcgg cagactatgg agtttt
//
LOCUS       DANLXS010000376         4384 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.guided.189, whole genome shotgun sequence.
ACCESSION   DANLXS010000376 DANLXS010000000
VERSION     DANLXS010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.guided.189"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..257
                     /locus_tag="PXD71_002753"
     CDS             <1..257
                     /locus_tag="PXD71_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL6292798.1"
                     /translation="DEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIE
                     DPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            726..1172
                     /locus_tag="PXD71_002754"
                     /pseudo
     CDS             726..1172
                     /locus_tag="PXD71_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
     gene            1449..2663
                     /gene="floR"
                     /locus_tag="PXD71_002755"
     CDS             1449..2663
                     /gene="floR"
                     /locus_tag="PXD71_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HDL6292799.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSVLAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNSDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            2691..2996
                     /locus_tag="PXD71_002756"
     CDS             2691..2996
                     /locus_tag="PXD71_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6292800.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
     gene            3108..>4384
                     /locus_tag="PXD71_002757"
     CDS             3108..>4384
                     /locus_tag="PXD71_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HDL6292801.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRAS"
BASE COUNT          844 a         1268 c         1350 g          922 t
ORIGIN      
        1 ctgatgaacc ggcaacacca gcacaacggc gagcgtcgat gacatgggcg caacggctca
       61 agcgtgtttt caatatcgac atcgagacct gcagcggctg cggcggcgcc atgaaagtca
      121 tcgcctgcat tgaagaccct atagtgatca agcagatcct tgatcacctg aagcacaaag
      181 ccgaaaccag cgggaccagg gcgttacccg aaagccgggc gccaccggct gagctgctcc
      241 tgggtctgtt tgactgacga gcctgaaggc caacgatacc aatcaaaatg ctgcgttcac
      301 agcgccgcgg cagggatccg ccgtgctggt tgtcggaaaa ggagccgcta gtgggaaaga
      361 ggagggtaaa ttttcagcgt tgctggctcc ccgtcagccg gattgggttg catcgcaggg
      421 gtgtcgaaag agtcaactgc ggtccaaagc tgttggactt gggtgaaaag ggcgtttatt
      481 cttcctatac gtgccgcgtt ccaggcggcg actatgaggg ctatgtcgat gcccatgtgc
      541 gccggctgga ggcgctacgc cgggccggta tcgtcgagcg gatcgacgcc gaccaatggc
      601 gcatccccga tgatctggtc agccgtgccg ccgcccatga cgccggccga gacagtcagg
      661 ccagcgttcg cgtcctttcc ccggtcgatc tgaacaaaca gatcggatcg gacggcgcga
      721 cctggctgga ccggcggctg atccacggcg agacggccga ccttgcgcca accggcttcg
      781 ggcaacaagt ccgcgaagcc atggaccagc gccgcgagca ccatatcgaa cagggcgacg
      841 ccacccgcag ccgggacagc cgcgtcttct accggcgcaa ccttctcgcc atcctgcggg
      901 agcgcgaggt agccggcgtc ggatcggata tggctttgag taagggcctg ccgttccgcg
      961 ccgccacgga cggcgagagc gtcagcggca agtttaccgg aaccgtgcat ctatcgagcg
     1021 gcaagttcgc cgtggtcgag aaatcccatg agttcaccct tgtcccgtgg cggccgatca
     1081 tcgaccgcca actcggccgc gaggttatgg gcatcgtgca gggcgggtcg gtgtcgtggc
     1141 agttagggcg gcagaggggg ctggaacgct gagtgcgccc atgccgcatt gcgaagcaaa
     1201 agataatcgg ataaaatgta gcaattcata ttcgtaagcg tggagtaatc agatgggaaa
     1261 ttccaagtca gcagacaagt aagccgcaac aaccagtatt gttgttgcgg cgctctgtaa
     1321 ggctagtctc atctgattgc tgacgagcag acgtcgcccg gtattcctta atcgagggtt
     1381 gattcgtcat gaccaccaca cgccccgcgt gggcctaaac aaatatcaat cgcaaggaga
     1441 tcatattcat gaccaccaca cgccccgcgt gggcctatac gctgccggca gcactgctgc
     1501 tgatggctcc tttcgacatc ctcgcttcac tggcgatgga tatttatctc cctgtcgttc
     1561 cagcgatgcc cggcatcctg aacacgacgc ccgctatgat ccaactcacg ttgagcctct
     1621 atatggtgat gctcggcgtg ggccaggtga tttttggtcc gctctcagac agaatcgggc
     1681 gacggccaat tctacttgcg ggcgcaacgg ctttcgtcat tgcgtctctg ggagcagctt
     1741 ggtcttcaac tgcaccggcc tttgtcgctt tccgtctact tcaagcagtg ggcgcgtcgg
     1801 ccatgctggt ggcgacgttc gcgacggttc gcgacgttta tgccaaccgt cctgagggtg
     1861 tcgtcatcta cggccttttc agttcggtgc tggcgttcgt gcctgcgctc ggccctatcg
     1921 ccggagcatt gatcggcgag ttcttgggat ggcaggcgat attcattact ttggctatac
     1981 tggcgatgct cgcactccta aatgcgggtt tcaggtggca cgaaacccgc cctctggatc
     2041 aagtcaagac gcgccgatct gtcttgccga tcttcgcgag tccggctttt tgggtttaca
     2101 ctgtcggctt tagcgccggt atgggcacct tcttcgtctt cttctcgacg gctccccgtg
     2161 tgctcatagg ccaagcggaa tattccgaga tcggattcag ctttgccttc gccactgtcg
     2221 cgcttgtaat gatcgtgaca acccgtttcg cgaagtcctt tgtcgccaga tggggcatcg
     2281 caggatgcgt ggcgcgtggg atggcgttgc ttgtttgcgg agcggtcctg ttggggatcg
     2341 gcgaacttta cggctcgccg tcattcctca ccttcatcct accgatgtgg gttgtcgcgg
     2401 tcggtattgt cttcacggtg tccgttaccg cgaacggcgc tttggcagag ttcgacgaca
     2461 tcgcgggatc agcggtcgcg ttctacttct gcgttcaaag cctgatagtc agcattgtcg
     2521 ggacattggc ggtggcactt ttaaacagtg acacagcgtg gcccgtgatc tgttacgcca
     2581 cggcgatggc ggtactggtt tcgttggggc tggtgctcct tcggctccgt ggggctgcca
     2641 ccgagaagtc gccagtcgtc taaccgacga ctggtagcag gcccgctccg atgcggcgca
     2701 ctaaccatcg aaacctcgtg aatgtcggta tcctgtctgg caggataccg ctcatttccc
     2761 ttgttcagtt catcgccgtc gccgagcatc tgaattttcg gcatgcggcc aaggcacttg
     2821 gtatcagcca gtcgagcgtc agcgcgcgtg tgaaagcgct ggaggataac cttggtgtcc
     2881 tgctatttga gcgccatgcg cggggcgttc ggctaacaga cgcaggcagg cacttcatgg
     2941 agcgtgtcac ggcgggtgtc gatcaactcg atcacgcagt gaagaccgcg gagtgacggg
     3001 cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg
     3061 ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg
     3121 cggccaggac ggccagccgg gatcgggata ctggtcgtta ccagagccac cgacccgagc
     3181 aaacccttct ctatcagatc gttgacgagt attacccggc attcgctgcg cttatggcag
     3241 agcagggaaa ggaattgccg ggctatgtgc aacgggaatt tgaagaattt ctccaatgcg
     3301 ggcggctgga gcatggcttt ctacgggttc gctgcgagtc ttgccacgcc gagcacctgg
     3361 tcgctttcag ctgtaagcgt cgcggtttct gcccgagctg tggggcgcgg cggatggccg
     3421 aaagtgccgc cttgctggtt gatgaagtac tgcctgaaca acccatgcgt cagtgggtgt
     3481 tgagcttccc gtttcagctg cgtttcctgt ttgccagccg gcccgagatc atggggtggg
     3541 tgctgggcat cgtttaccgc gtcattgcca cgcacctggt caagaaagcg ggccataccc
     3601 accaagtggc caagacgggc gcggtcaccc tgatccagcg ttttggatcg gcgctcaatc
     3661 tgaatgttca cttccacatg ctgtttctcg acggtgtgta tgtcgagcaa tcccacggct
     3721 cagcgcgttt ccgctgggtc aaggcgccga ccagcccaga gctcacccag ctgacgcaca
     3781 ccatcgccca ccgggtgggt cgctatctgg aacggcaagg cctgctggaa cgggatgtcg
     3841 aaaacagcta tctggcctcg gatgcggtgg atgacgaccc gatgacaccc ctgctggggc
     3901 actcgatcac ttaccgtatc gctgtcggtt cacaggcggg gcgaaaggtg ttcactttgc
     3961 aaactctgcc gaccagtggt gatccgttcg gtgacgggat tggcaaggta gccgggtcca
     4021 gcctgcacgc cggcgtggcg gccagggccg atgaacgcaa gaagctcgaa cggctgtgcc
     4081 ggtacatcag ccgcccggcg gtatccgaga agcggctgtc gttaacacga ggcggcaacg
     4141 tgcgctacca gctcaagacg ccgtaccggg acggcaccac gcacgtcatt ttcgaaccat
     4201 tggatttcat tgcaaggctg gccgccctgg taccgaagcc cagagtcaac ctaacccgct
     4261 tccacggggt gttcgcaccc aacagtcggc accgggcgtt ggtcacgccg gcaaaacggg
     4321 gcaggggcaa caaggtcagg gtggctgatg aaccggcaac accagcacaa cggcgagcgt
     4381 cgat
//
LOCUS       DANLXS010000377         4384 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.guided.190, whole genome shotgun sequence.
ACCESSION   DANLXS010000377 DANLXS010000000
VERSION     DANLXS010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.guided.190"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..257
                     /locus_tag="PXD71_002758"
     CDS             <1..257
                     /locus_tag="PXD71_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL6292802.1"
                     /translation="DEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIE
                     DPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            726..1172
                     /locus_tag="PXD71_002759"
                     /pseudo
     CDS             726..1172
                     /locus_tag="PXD71_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
     gene            1449..2663
                     /gene="floR"
                     /locus_tag="PXD71_002760"
     CDS             1449..2663
                     /gene="floR"
                     /locus_tag="PXD71_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HDL6292803.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSVLAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNSDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            2691..2996
                     /locus_tag="PXD71_002761"
     CDS             2691..2996
                     /locus_tag="PXD71_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6292804.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
     gene            3108..>4384
                     /locus_tag="PXD71_002762"
     CDS             3108..>4384
                     /locus_tag="PXD71_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HDL6292805.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRAS"
BASE COUNT          843 a         1269 c         1350 g          922 t
ORIGIN      
        1 ctgatgaacc ggcaacacca gcacaacggc gagcgtcgat gacatgggcg caacggctca
       61 agcgtgtttt caatatcgac atcgagacct gcagcggctg cggcggcgcc atgaaagtca
      121 tcgcctgcat tgaagaccct atagtgatca agcagatcct tgatcacctg aagcacaaag
      181 ccgaaaccag cgggaccagg gcgttacccg aaagccgggc gccaccggct gagctgctcc
      241 tgggtctgtt tgactgacga gcctgaaggc caacgatacc aatcaaaatg ctgcgttcac
      301 agcgccgcgg cagggatccg ccgtgctggt tgtcggaaaa ggagccgcta gtgggaaaga
      361 ggagggtaaa ttttcagcgt tgctggctcc ccgtcagccg gattgggttg catcgcaggg
      421 gtgtcgaaag agtcaactgc ggtccaaagc tgttggactt gggtgaaaag ggcgtttatt
      481 cttcctatac gtgccgcgtt ccaggcggcg actatgaggg ctatgtcgat gcccatgtgc
      541 gccggctgga ggcgctacgc cgggccggta tcgtcgagcg gatcgacgcc gaccaatggc
      601 gcatccccga tgatctggtc agccgtgccg ccgcccatga cgccggccga gacagtcagg
      661 ccagcgttcg cgtcctttcc ccggtcgatc tgaacaaaca gatcggatcg gacggcgcga
      721 cctggctgga ccggcggctg atccacggcg agacggccga ccttgcgcca accggcttcg
      781 ggcaacaagt ccgcgaagcc atggaccagc gccgcgagca ccatatcgaa cagggcgacg
      841 ccacccgcag ccgggacagc cgcgtcttct accggcgcaa ccttctcgcc atcctgcggg
      901 agcgcgaggt agccggcgtc ggatcggata tggctttgag taagggcctg ccgttccgcg
      961 ccgccacgga cggcgagagc gtcagcggca agtttaccgg aaccgtgcat ctatcgagcg
     1021 gcaagttcgc cgtggtcgag aaatcccatg agttcaccct tgtcccgtgg cggccgatca
     1081 tcgaccgcca actcggccgc gaggttatgg gcatcgtgca gggcgggtcg gtgtcgtggc
     1141 agttagggcg gcagaggggg ctggaacgct gagtgcgccc atgccgcatt gcgaagcaaa
     1201 agataatcgg ataaaatgta gcaattcata ttcgtaagcg tggagtaatc agatgggaaa
     1261 ttccaagtca gcagacaagt aagccgcaac aaccagtatt gttgttgcgg cgctctgtaa
     1321 ggctagtctc atctgattgc tgacgagcag acgtcgcccg gtattcctta atcgagggtt
     1381 gattcgtcat gaccaccaca cgccccgcgt gggcctaaac aaatatcaat cgcaaggaga
     1441 tcatattcat gaccaccaca cgccccgcgt gggcctatac gctgccggca gcactgctgc
     1501 tgatggctcc tttcgacatc ctcgcttcac tggcgatgga tatttatctc cctgtcgttc
     1561 cagcgatgcc cggcatcctg aacacgacgc ccgctatgat ccaactcacg ttgagcctct
     1621 atatggtgat gctcggcgtg ggccaggtga tttttggtcc gctctcagac agaatcgggc
     1681 gacggccaat tctacttgcg ggcgcaacgg ctttcgtcat tgcgtctctg ggagcagctt
     1741 ggtcttcaac tgcaccggcc tttgtcgctt tccgtctact tcaagcagtg ggcgcgtcgg
     1801 ccatgctggt ggcgacgttc gcgacggttc gcgacgttta tgccaaccgt cctgagggtg
     1861 tcgtcatcta cggccttttc agttcggtgc tggcgttcgt gcctgcgctc ggccctatcg
     1921 ccggagcatt gatcggcgag ttcttgggat ggcaggcgat attcattact ttggctatac
     1981 tggcgatgct cgcactccta aatgcgggtt tcaggtggca cgaaacccgc cctctggatc
     2041 aagtcaagac gcgccgatct gtcttgccga tcttcgcgag tccggctttt tgggtttaca
     2101 ctgtcggctt tagcgccggt atgggcacct tcttcgtctt cttctcgacg gctccccgtg
     2161 tgctcatagg ccaagcggaa tattccgaga tcggattcag ctttgccttc gccactgtcg
     2221 cgcttgtaat gatcgtgaca acccgtttcg cgaagtcctt tgtcgccaga tggggcatcg
     2281 caggatgcgt ggcgcgtggg atggcgttgc ttgtttgcgg agcggtcctg ttggggatcg
     2341 gcgaacttta cggctcgccg tcattcctca ccttcatcct accgatgtgg gttgtcgcgg
     2401 tcggtattgt cttcacggtg tccgttaccg cgaacggcgc tttggcagag ttcgacgaca
     2461 tcgcgggatc agcggtcgcg ttctacttct gcgttcaaag cctgatagtc agcattgtcg
     2521 ggacattggc ggtggccctt ttaaacagtg acacagcgtg gcccgtgatc tgttacgcca
     2581 cggcgatggc ggtactggtt tcgttggggc tggtgctcct tcggctccgt ggggctgcca
     2641 ccgagaagtc gccagtcgtc taaccgacga ctggtagcag gcccgctccg atgcggcgca
     2701 ctaaccatcg aaacctcgtg aatgtcggta tcctgtctgg caggataccg ctcatttccc
     2761 ttgttcagtt catcgccgtc gccgagcatc tgaattttcg gcatgcggcc aaggcacttg
     2821 gtatcagcca gtcgagcgtc agcgcgcgtg tgaaagcgct ggaggataac cttggtgtcc
     2881 tgctatttga gcgccatgcg cggggcgttc ggctaacaga cgcaggcagg cacttcatgg
     2941 agcgtgtcac ggcgggtgtc gatcaactcg atcacgcagt gaagaccgcg gagtgacggg
     3001 cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg
     3061 ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg
     3121 cggccaggac ggccagccgg gatcgggata ctggtcgtta ccagagccac cgacccgagc
     3181 aaacccttct ctatcagatc gttgacgagt attacccggc attcgctgcg cttatggcag
     3241 agcagggaaa ggaattgccg ggctatgtgc aacgggaatt tgaagaattt ctccaatgcg
     3301 ggcggctgga gcatggcttt ctacgggttc gctgcgagtc ttgccacgcc gagcacctgg
     3361 tcgctttcag ctgtaagcgt cgcggtttct gcccgagctg tggggcgcgg cggatggccg
     3421 aaagtgccgc cttgctggtt gatgaagtac tgcctgaaca acccatgcgt cagtgggtgt
     3481 tgagcttccc gtttcagctg cgtttcctgt ttgccagccg gcccgagatc atggggtggg
     3541 tgctgggcat cgtttaccgc gtcattgcca cgcacctggt caagaaagcg ggccataccc
     3601 accaagtggc caagacgggc gcggtcaccc tgatccagcg ttttggatcg gcgctcaatc
     3661 tgaatgttca cttccacatg ctgtttctcg acggtgtgta tgtcgagcaa tcccacggct
     3721 cagcgcgttt ccgctgggtc aaggcgccga ccagcccaga gctcacccag ctgacgcaca
     3781 ccatcgccca ccgggtgggt cgctatctgg aacggcaagg cctgctggaa cgggatgtcg
     3841 aaaacagcta tctggcctcg gatgcggtgg atgacgaccc gatgacaccc ctgctggggc
     3901 actcgatcac ttaccgtatc gctgtcggtt cacaggcggg gcgaaaggtg ttcactttgc
     3961 aaactctgcc gaccagtggt gatccgttcg gtgacgggat tggcaaggta gccgggtcca
     4021 gcctgcacgc cggcgtggcg gccagggccg atgaacgcaa gaagctcgaa cggctgtgcc
     4081 ggtacatcag ccgcccggcg gtatccgaga agcggctgtc gttaacacga ggcggcaacg
     4141 tgcgctacca gctcaagacg ccgtaccggg acggcaccac gcacgtcatt ttcgaaccat
     4201 tggatttcat tgcaaggctg gccgccctgg taccgaagcc cagagtcaac ctaacccgct
     4261 tccacggggt gttcgcaccc aacagtcggc accgggcgtt ggtcacgccg gcaaaacggg
     4321 gcaggggcaa caaggtcagg gtggctgatg aaccggcaac accagcacaa cggcgagcgt
     4381 cgat
//
LOCUS       DANLXS010000378         3710 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.guided.216, whole genome shotgun sequence.
ACCESSION   DANLXS010000378 DANLXS010000000
VERSION     DANLXS010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.guided.216"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..650
                     /locus_tag="PXD71_002763"
     CDS             <1..650
                     /locus_tag="PXD71_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL6292806.1"
                     /translation="KAAKEKAEAERLAAEKEVDPIAIIKAKGIDDTNYGLKLGNSKET
                     IEGKVTVRTQSYLYNQPYSVVTAQAVQRNGWENGNYIDKVDGTVTVKGLKTEKLPTEG
                     TATYSGKAFNHDGAKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLIVLEEGTINNTQIS
                     STVHGNNPLNLSGKYTVEFFGKNAEEIAGEIQTKANLTGEVSNFGLAGTRGDIQK"
     gene            846..2255
                     /locus_tag="PXD71_002764"
     CDS             846..2255
                     /locus_tag="PXD71_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6292807.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            2401..2874
                     /gene="gpt"
                     /locus_tag="PXD71_002765"
     CDS             2401..2874
                     /gene="gpt"
                     /locus_tag="PXD71_002765"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL6292808.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            2987..3610
                     /gene="gmk"
                     /locus_tag="PXD71_002766"
     CDS             2987..3610
                     /gene="gmk"
                     /locus_tag="PXD71_002766"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL6292809.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
BASE COUNT         1112 a          700 c          852 g         1046 t
ORIGIN      
        1 aaaaagcggc gaaagagaaa gcggaagcgg agcgtttagc agcagaaaaa gaagtagatc
       61 ctatagctat tattaaagca aaaggtattg atgatacaaa ttacggctta aaacttggta
      121 atagtaaaga aactattgag ggtaaagtca cagtcagaac acagagttat ttatataatc
      181 agccatattc tgtagtaaca gcacaagcag ttcaaagaaa tggctgggaa aacggtaatt
      241 atattgataa agttgatgga acagttacag ttaaaggatt gaaaacagag aaattaccaa
      301 cagagggtac tgcaacatat tcaggaaaag catttaacca cgatggagct aaaggtttta
      361 atggtggtga tctaacctat aatgttaatt tctctaagcg taccggttca ggtagagttg
      421 agggagaatt tggaaactta atcgtattag aagaggggac gattaataat actcagattt
      481 cttcaacagt acatggcaat aatcctttaa acctttctgg aaaatataca gtcgaatttt
      541 tcggtaaaaa tgcagaggaa attgccggtg aaattcagac aaaagcaaat cttactgggg
      601 aagtgagtaa ttttggactt gccggcactc gtggcgatat ccaaaaataa ccttttctaa
      661 cgttcaatgc cgtttaacga aaaattaaac ggcatttgtc ttgatattgc ttgtagatac
      721 cataagacac aatagtttag atttcaattt acagcttcta ataaccctct cccacaaggg
      781 gagagagtga taaatcccgt agaggcgaac atataggtcc gcccctacca attttcttta
      841 tctttatgaa aaaatatccc cttttatttt cgctgttcag ccccgttgta tgggcagaaa
      901 aggtgattga acacaacact gacccgattt cctttgagcg tcagccgcaa gcggttaaat
      961 ttgagcaaaa tgttgtaaaa aattcacaaa aaccgaccgc ttatcagcct gcgtattcag
     1021 tcaatcagaa tgttgaacag caaattaacc aagcgattct cagccaagat tgggcaacgc
     1081 ttgaaccgct tttaagccgt tatccgcaaa ctgaaaattt tgacccgttg ttgtggcact
     1141 atgcgcaagc cgcacttgcc tatgcgaaaa aagatcatca aaccgcagtt tttcactatc
     1201 gccaactgtt agctaagccc ccgagtttgg tttaccctcg ttttgatttg gcgttgattt
     1261 tggcagaaga tcagcaattt cgtgcggcag agcaggaatt taaggctatt caagcccatt
     1321 taccgccgga acttgctaaa attgcccgca atactcaagt ccaaattgct gaaaatgagg
     1381 cgtggcagcc cgatttttat ctgcaatata cccaaacaga taacgtgaat aatgcctcat
     1441 cttcgccgat tgtgaatgta aatgggcgga ttttccgcaa aaatgcagac agtttgccac
     1501 aatcagcaaa aggcgtgcgt tatggcttag ggttgagtaa attgcacaac gtgaaaggca
     1561 atcattttgt cggtttaacg ctcaattata gcgggattta ctattgggat aaccgagatt
     1621 accgtgagca atccgtatcc gtttcgccga attatagcta ccgcaccgtc gcttatcggc
     1681 tgactttttc gccttttatt gagcaaaatt ggctggggag tagccgttat aatgttcaat
     1741 ttggggcaac actcaacggt ttgcgttacc ttaattcgca atggttggtg tcggggagtt
     1801 ttacccattt gcaaaaacgg tattttgagg agctaaccgc aagccgttat aacagctatc
     1861 agcaccaact cagtggcggt gtgcagtggc aagcggtcaa aaatgggcgg ttttttgcaa
     1921 atttagacgg cgtgcgtgaa attgcacggg aaaaagcaca aagctcgaat aagtggctgg
     1981 ctcgagtggg ggcggttttt caaggcgaac gctgggcaac gcaggcaagt atcggctttg
     2041 gcaagcgata ttttgccgac aagcactatt tgtttggcta taagcggtta gacacggaat
     2101 atcaagccaa tttagcggtt tggaaccgca cttggcattg gcagggcatt gtgccgaagc
     2161 tgaatttccg ttatcaaaaa attgacagca atattgctga tttttactct cgccaaaaca
     2221 aggaagtctt tttcacttta gaaaagctat tttagtgttg gttttttgtg ctaagcatac
     2281 gattgcgtag ctgccggcac aaaaaaataa atgaaatgat ggcgaaaaaa gcattttcgg
     2341 gttatcctag tgcaattttt tatatcgggg tagcccccaa ttttatatta ggttgtttct
     2401 atgagtgaaa aatacacaaa tgaaaaatat gtcgtgacgt gggatatgtt ccatatgcac
     2461 gctcgcaagt tggcagaaaa tttattacct gccacacagt ggaaagggat tattgcggtg
     2521 agccgtggcg gcttgtttcc ggcagccgtt ttagcacgtg agttgggtat tcgccacgta
     2581 gaaacagtgt gcattgccag ctacgatcac gatagccaag gtgagttaaa tgtgttacac
     2641 gcagccgata ccgatggcga aggctttatt gtggtagatg atttagtgga taccggcaac
     2701 acggcaaaag aaattcgcag aatgtacccg aaagctcgtt ttgtaaccgt gtttgcgaaa
     2761 ccgcacggtg caccgttggt tgatgattat gtgattgata ttccacaaga aacgtggatt
     2821 gagcagcctt gggatttagg cattaccttc gttcagcctt tggcacgtag ataattttgt
     2881 gtaatggaca atgaaagttg tccattattt tttatttatt cctatgaaat gtaggggcga
     2941 atcatcattc gccctattcc caaaatcaaa taggtatata caaataatga gtttaggcaa
     3001 tctttatatt atttctgccc caagtggagc aggaaaatct tccttaatta acgccttgtt
     3061 ggcggattta ccccgttcgg aagtacagct ttcgatttca cacactaccc gtcagcctcg
     3121 tccggcagaa caggacggcg tgcattatta tttcacttcg cacgatgagt ttaagcagct
     3181 gattgagcaa gggtattttt tagaatgggc ggaggtgttt ggcaattatt acggcacatc
     3241 attgccgatg attgaacgta gcctagagca gggcattgat gtctttttgg atattgactg
     3301 gcaaggggct agacaaattc gggaaaaagt gccgaatgtg aaaaccgttt ttattttgcc
     3361 accgtcaaaa acagaattgg aaaaacgttt aatcgggcgt gggcaagatt ccgctgaaac
     3421 cattgccaaa cgtatggcag aggcggtgtc ggaaatgaac cattacaatg agttcgatta
     3481 tgtgattatt aatgatgatt tccaacaggc attggcagag ctaaaagcga ttttaattgc
     3541 cgagcgtttg agcttaggtt cgcaacaaat tcgtcatcag catttgatta ctgatttgtt
     3601 aaataattaa atgaacagtt gttcatttaa ctggaaaggc aatctgtttt tgggtagaat
     3661 acgcccattt tcccctcttt attttcaaaa ggaaatgtaa tgaaactttc
//
LOCUS       DANLXS010000379         3417 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.guided.227, whole genome shotgun sequence.
ACCESSION   DANLXS010000379 DANLXS010000000
VERSION     DANLXS010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.guided.227"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            87..1289
                     /gene="tet(H)"
                     /locus_tag="PXD71_002767"
     CDS             87..1289
                     /gene="tet(H)"
                     /locus_tag="PXD71_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL6292810.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
     gene            complement(1385..2695)
                     /locus_tag="PXD71_002768"
     CDS             complement(1385..2695)
                     /locus_tag="PXD71_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL6292811.1"
                     /translation="MSNILNWPDYKVLQVSELEHDYQVHAEVSEPPTQCPHCNHPEIV
                     GFGRRDEVIMDTPVHGRRTGIMLNRRRYRCQSCRKTFLEPVPHKDEKRQMTNRLIQYI
                     ERESLRRTFSSVAEDVGVDEKTVRNIFNDYCERLEKTLNFEMPQWLGIDEIHIIKPRC
                     VITNIQQQTIVDMLDNRNKTTVTRYLSKRTDRDLVRYVAMDMWRPYRQAVETMIPDAT
                     VIIDKFHVVRMANESLERARKAIRSALTPQQRRGLMRDRFVLLKRRHELTDAEYMRFS
                     GWTLNYPEIGQAYELKEAFFEIWDCQTRHQAQEAYYSWLRQITPEMKAHYDPLIKAMG
                     NWHDDIFAYFDHPITNAYTESLNNLIRVVNRVGRGYSFEALRAKILFTEGFQKIKKPR
                     YQRQRIPEGAMGRMPFYGVAEAGPSTNYGADISTLVREIEAGRL"
     gene            complement(2695..2997)
                     /locus_tag="PXD71_002769"
     CDS             complement(2695..2997)
                     /locus_tag="PXD71_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292812.1"
                     /translation="MKIKLYFPDESIATIKRMGLRQMSPETLRWTADHPSSSYGMGAL
                     LRGKSGEILDGKSFAAMVHAFGAWIETDSEDTSRRVHNALVTAATGTEESVKVAKE"
     gene            complement(3038..3226)
                     /locus_tag="PXD71_002770"
     CDS             complement(3038..3226)
                     /locus_tag="PXD71_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292813.1"
                     /translation="MSYDANDALNEIEEALSELERVAEDLINNNPNKESELRGQGVHQ
                     ATKHLRFRIRNIRRGEAI"
BASE COUNT          802 a          819 c          806 g          990 t
ORIGIN      
        1 tacctcaaaa taaaatagcc ttgacaatct atcactgata agatatatta cacccactct
       61 atcactgata gatttttagg atctcaatga ataaatcaat tattattata ctgctgatca
      121 ccgtattaga tgccattggt atcgggctta tcatgccagt actccctact ctattaaatg
      181 aatttgtcag tgaaaattca ctggcaaccc attacggtgt gctattagcg ctctatgcta
      241 ccatgcaggt tatttttgct cctattctag gacgactgtc tgataaatac ggcagaaaac
      301 ccatcttgct gttttccctt ttaggcgcgg cactcgacta tcttttaatg gcattctcaa
      361 ccacactttg gatgctctat attgggcgca tcattgcggg gatcacaggc gcaacaggtg
      421 ccgtatgtgc atcagcgatg agtgatgtga ctcccgctaa aaatcgaact cgctattttg
      481 gtttcttagg tggtgctttt ggtgttggcc ttattatcgg cccaatgcta gggggattat
      541 taggtgatat cagtgctcat atgccattta tttttgccgc tatttcacac tcgatattat
      601 taatactctc tttgctcttt ttccgagaaa cacaaaaaag agaagcgctt gttgccaata
      661 ggacacctga aaaccaaact gcctcaaata cagtcactgt tttttttaag aaaagcctct
      721 acttttggtt agcaacctat tttattatcc agcttatcgg gcaaattcct gccaccatct
      781 gggtgctgtt tacacaatat cgttttgatt ggaacacaac ttctatcggt atgtctttgg
      841 cggttctggg tgtattaaat attttctttc aggcgattgt cgctgggaaa ttggcacaaa
      901 aatggggcga aaaaaccacc attatgatca gtatgtctat tgatatgatg ggctgtttat
      961 tattagcgtg gataggccac gtttgggtca tcttaccagc attaatttgc ttagcggcag
     1021 gaggtatggg gcaacccgca ttacaaggtt atttatcaaa atctgtcgat gataatgcgc
     1081 aagggaaatt acaaggtact ctggtgagcc taaccaatat taccgggatc attggtcccc
     1141 ttttatttgc ctttatttat agttatagcg tcgcttattg ggatggtctg ttatggctga
     1201 tgggggcaat actttatgct atgttgctta ttaccgctta ttttcaccaa agaaaaacca
     1261 cacctaaagc tgttatttca accccttaat actagaaatt ataaagatca taaaagtcct
     1321 gtccgctctt aataacgttg ataggacttt atttgggtat ccggattaca gggttgaaac
     1381 agggctataa acgccccgcc tcaatctccc gcaccagtgt tgatatgtca gcgccgtagt
     1441 tagtggatgg ccccgcttcg gcaacgccat agaacggcat cctgcccata gcgccctcgg
     1501 gtatccgttg acgctgatag cgtggcttct tgatcttctg gaagccctca gtaaacagga
     1561 tcttggctcg cagcgcttca aagctgtagc cacggccaac acgattaaca acacgaatca
     1621 ggttgttcag tgactcggtg taggcgttgg taatggggtg gtcgaagtaa gcgaaaatgt
     1681 catcgtgcca gttgcccatc gctttgataa gcgggtcata gtgggctttc atttccggcg
     1741 taatctgtcg cagccaagag taatatgcct cctgtgcctg gtgccgggtc tgacagtccc
     1801 aaatctcgaa aaaggcttct ttcagttcgt aggcttggcc aatctcaggg taattcaatg
     1861 tccagcctga aaaacgcatg tattcggcat ctgtcagctc gtgacggcgt ttcagtagga
     1921 caaacctatc tcgcatcaac ccacggcgct gctgtggcgt cagggcgctt ctgatggctt
     1981 ttctggcgcg ttccagtgat tcgttcgcca tcctgacaac gtggaacttg tcgatgatca
     2041 ccgtggcgtc aggaatcatg gtttctactg cttgccggta cggcctccac atatccattg
     2101 ccacgtagcg caccaggtcg cggtcagtgc gtttgctcag gtagcgggtg acggtggttt
     2161 tattgcggtt atccagcatg tccacaatgg tctgctgctg aatgttggtg atcacgcagc
     2221 ggggtttgat gatgtggatt tcgtcgatac ccaaccactg cggcatttca aaattgagcg
     2281 tcttttcgag gcgttcacaa tagtcgttga aaatgttacg gacggtcttt tcatcgacgc
     2341 ccacatcctc ggcaacactg gagaacgtgc ggcgcaggct ctcgcgctcg atgtactgga
     2401 tcaggcggtt ggtcatctgc cgcttttcgt ctttgtgcgg tacaggctcc agaaacgtct
     2461 ttcggcaaga ctggcagcga tagcgacggc ggttaagcat gatgccggta cgccttccgt
     2521 ggacgggtgt atccatgatc acctcatcac ggcgaccaaa gccgacgatt tcaggatgat
     2581 tgcagtgggg gcactgcgta ggcggttcgg aaacttcggc gtgtacctgg taatcgtgct
     2641 ccagttcgga tacctggagc actttgtaat caggccagtt gaggatattc gacattactc
     2701 ctttgccacc ttaaccgatt cctcggtgcc agtggcagca gtgactagcg cgttatgaac
     2761 tcgacgactg gtatcttcac tgtctgtttc aatccacgcg ccaaaggcat gcaccattgc
     2821 agcaaaagac ttcccatcaa ggatttcacc agacttgcct ctgagcaaag cgcccatacc
     2881 atagctgctt gacggatgat cggcagtcca cctgagagtc tcgggagaca tttggcgaag
     2941 ccccattcgc tttatcgtgg cgatagactc gtccggaaaa tagagtttga ttttcatgct
     3001 gacctcgctg aaagccgccc ctagggcggc atgttagtca aattgcttcg ccgcggcgga
     3061 tgttgcgaat acgaaaacgg aggtgctttg tcgcttggtg aaccccttgg ccgcgtaatt
     3121 ctgattcttt attcgggttg ttgttgatta ggtcttcagc aaccctttca agctcgctaa
     3181 gcgcctcttc gatttcattc agcgcatcat ttgcatcgta tgacatggct tctatcctgc
     3241 accaagttcg gcggtgcgcc cgttgggctt atgcccgagc cgttgttctc agctcttgaa
     3301 tctaatatta gggcatctgg ccttttccgt caaccctaaa atccggttta tttacaaaat
     3361 tacgtctagc gcagatcaac cgctaaatcc ggatacccaa aatatcatat ataaata
//
LOCUS       DANLXS010000380         3226 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.guided.241, whole genome shotgun sequence.
ACCESSION   DANLXS010000380 DANLXS010000000
VERSION     DANLXS010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.guided.241"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            939..1076
                     /locus_tag="PXD71_002771"
     CDS             939..1076
                     /locus_tag="PXD71_002771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6292814.1"
                     /translation="MKPLRIGARVIYPLQDLDKQIAYLEANLLENIKVHFIVESIEIK
                     G"
     gene            1211..2128
                     /locus_tag="PXD71_002772"
     CDS             1211..2128
                     /locus_tag="PXD71_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class A beta-lactamase
                     ROB-1"
                     /protein_id="HDL6292815.1"
                     /translation="MLNKLKIGTLLLLTLTACSPNSVHSVTSNPQPASAPVQQSATQA
                     TFQQTLANLEQQYQARIGVYVWDTETGHSLSYRADERFAYASTFKALLAGAVLQSLPE
                     KDLNRTISYSQKDLVSYSPETQKYVGKGMTIAQLCEAAVRFSDNSATNLLLKELGGVE
                     QYQRILRQLGDNVTHTNRLEPDLNQAKPNDIRDTSTPKQMAMNLNAYLLGNTLTESQK
                     TILWNWLDNNATGNPLIRAATPTSWKVYDKSGAGKYGVRNDIAVVRIPNRKPIVMAIM
                     STQFTEEAKFNNKLVEDAAKQVFHTLQLN"
     gene            2139..3056
                     /locus_tag="PXD71_002773"
     CDS             2139..3056
                     /locus_tag="PXD71_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL6292816.1"
                     /translation="MKKKLLWILILGLIIISCKQRKTEMKEKIIKTNGIELCTESFGN
                     KKNPAILLVAGATVSMLYWDTEFCQQLSEKGFFVIRYDNRDVGKSTNYEPGSTPYDIV
                     DLTNDAISILDGYKIDKAHFVGISLGGLISQIASIKFADRVNSLTLMSSGPWGDSDPT
                     IPEMDTSILDFHSKAGTVNWTNEDSVVNYLIQGAELMSGKKQFDKQRSEKLIRAEFNR
                     ANNYISMFNHAALQGGGEEYWNRLNEIKQPTLIIHGTDDKIWHYKNAGFLLEKIKGSN
                     LITLEGTGHELHVDDWKSIIDGIEKHIND"
BASE COUNT         1050 a          605 c          656 g          915 t
ORIGIN      
        1 gtggttattg tgggcggtag gtttttagtg ttggaaagct gttggaaagc tcgtgggtaa
       61 ctcgcagagt tatccatcag cttttcaatg gcttttcaat acgacccgca ggggaaaaac
      121 cgttaaaccg tccacattaa ccgctgaccc aaaaagaacg ctttttacta aaagatttct
      181 attcggttct caaatgttgc gtgaatgggg caacaataaa acgttgtggc gtttatcagg
      241 gtgggaattg cagacttaaa caatcaattc cgctgtatcc ttaaatctcg cttatcaggt
      301 gtgcttgtat ttcttggggc tttcgccttt ctaaccgtag cacgaaacgg agacagagct
      361 taattttcgt attaagaacg ttatcacttc tacgcctgtt tattcttccg aagtccgagc
      421 tgaagaccgt ctaatttaag gtttacgttt cttgtttacg tcgttgaatg gttttttggt
      481 agtagggacg cttcgcaatc tcttgcatta ctagctaccc tttgctatga ttagattgtt
      541 cacgtctgga tcatagcaaa gaacgtactg atacagatta taggaaaagg gatcgaatgt
      601 caaaagttcg atcccttttt cttttctaaa gcattggtat taaaggcttt aaggtgtatt
      661 ttattaaaat ctgtacacct ttgtgaaaat ccatacacct ttcttaccgt gtaattttat
      721 gaaaattcgt gcacctttct gctaacgccc tgcggttgtt agactaaaaa agccattgaa
      781 agcaagtttc aacggctttt tcagtcccga ataaaacaaa accgccttgt taggcggtta
      841 tgtgttaaaa tcactttcgc actatggcgg cggctcgcac taccgttcat agtgtgagcc
      901 tagtcgctta gccttatagt gctaaggggg tgatttatgt gaaacctcta cgaattgggg
      961 caagggttat ttatccgctt caagacttag acaagcaaat tgcataccta gaggcgaact
     1021 tacttgagaa tatcaaagtg catttcattg tagaaagcat tgagattaaa ggctaaattg
     1081 ctagactttt gcgcttaaat tcgccaaaat ctgttttttt gttctacaaa caaattatcc
     1141 gaatttccgc gcaatgtacg tttcaggctg cctgaaaaga aatttattta gtaaaatcaa
     1201 aggataattt atgttaaata agttaaaaat cggcacatta ttattgctga cattaacggc
     1261 ttgttcgccc aattctgttc attcggtaac gtctaatccg cagcctgcta gtgcgcctgt
     1321 gcaacaatca gccacacaag ccacctttca acagactttg gcgaatttgg aacagcagta
     1381 tcaagcccga attggcgttt atgtatggga tacagaaacg ggacattctt tgtcttatcg
     1441 tgcagatgaa cgctttgctt atgcgtccac tttcaaggcg ttgttggctg gggcggtgtt
     1501 gcaatcgctg cctgaaaaag atttaaatcg taccatttca tatagccaaa aagatttggt
     1561 tagttattct cccgaaaccc aaaaatacgt tggcaaaggc atgacgattg cccaattatg
     1621 tgaagcagcc gtgcggttta gcgacaacag cgcgaccaat ttgctgctca aagaattggg
     1681 tggcgtggaa caatatcaac gtattttgcg acaattaggc gataacgtaa cccataccaa
     1741 tcggctagaa cccgatttaa atcaagccaa acccaacgat attcgtgata cgagtacacc
     1801 caaacaaatg gcgatgaatt taaatgcgta tttattgggc aacacattaa ccgaatcgca
     1861 aaaaacgatt ttgtggaatt ggttggacaa taacgcaaca ggcaatccat tgattcgcgc
     1921 tgctacgcca acatcgtgga aagtgtacga taaaagcggg gcgggtaaat atggtgtacg
     1981 caatgatatt gcggtggttc gcataccaaa tcgcaaaccg attgtgatgg caatcatgag
     2041 tacgcaattt accgaagaag ccaaattcaa caataaatta gtagaagatg cagcaaagca
     2101 agtatttcat actttacagc tcaactaaca aattcagcat gaaaaaaaaa ctactttgga
     2161 tattaatttt aggactgata ataatcagtt gcaaacaaag gaaaacagaa atgaaagaga
     2221 aaataattaa aacaaacggc attgaactct gtacggaaag ttttggaaat aagaaaaatc
     2281 cagcaatcct tttggtagca ggtgcaaccg tatcaatgct gtattgggac actgaatttt
     2341 gccaacaatt atctgaaaaa ggattttttg ttattcgtta cgacaacaga gatgtaggaa
     2401 aatccactaa ttatgaacca ggttctactc catacgatat tgttgactta actaatgacg
     2461 ctatttcaat attggatggc tacaagattg acaaagcaca ttttgtgggg atttctttgg
     2521 gcggactaat ttctcaaata gcatcaataa agtttgccga cagagttaac tccttaactc
     2581 ttatgtcatc aggcccttgg ggagactcag acccaactat acctgaaatg gacacgagta
     2641 ttttagattt ccatagtaaa gcaggtacag tcaattggac aaatgaagac agtgtggtaa
     2701 actatttaat tcagggtgca gaattaatga gtggcaagaa acaatttgac aaacaaagaa
     2761 gtgaaaaact gataagagct gagttcaata gagccaacaa ttatataagt atgttcaatc
     2821 acgctgcatt gcaaggtggt ggtgaagaat attggaacag attaaacgaa atcaaacaac
     2881 ccaccttaat tatccacgga acagacgaca aaatttggca ttataagaat gcaggttttt
     2941 tactagaaaa aataaaaggt tcaaatctaa tcacccttga aggtacagga cacgaattac
     3001 acgttgatga ttggaaatca ataattgatg gaatagaaaa acacataaat gactgataaa
     3061 caaaaacaac gacccgctaa caatgtatat aaaaaatagg cgaaacagta gtaaattcaa
     3121 ggcttttggc tcgtattata ctttgtgctt aaccgaaaat ttggcacttc gaaatcgcct
     3181 acttttcgct gaatttacaa tccaagtgca acaaaaaaag aagtac
//
LOCUS       DANLXS010000381         2544 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.guided.258, whole genome shotgun sequence.
ACCESSION   DANLXS010000381 DANLXS010000000
VERSION     DANLXS010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.guided.258"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            69..1544
                     /gene="msr(E)"
                     /locus_tag="PXD71_002774"
     CDS             69..1544
                     /gene="msr(E)"
                     /locus_tag="PXD71_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(E)"
                     /protein_id="HDL6292817.1"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
     gene            1600..2484
                     /locus_tag="PXD71_002775"
     CDS             1600..2484
                     /locus_tag="PXD71_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="HDL6292818.1"
                     /translation="MTIQDIQSLAEAHGLLITDKMNFNEMGIDFKVVFALDTKGQQWL
                     LRIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAE
                     TYEIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLV
                     KSEIGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAH
                     IDDPAIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFAL
                     ETQNESLIVGAKAQLGVI"
BASE COUNT          868 a          418 c          546 g          712 t
ORIGIN      
        1 tagtttccag ttataactta attgactagc tattaaaaat ttcaagagct aaacaggagt
       61 aaataaaaat gagtttaatt attaaagcga gaaacatacg cttggattat gctgggcgtg
      121 atgttttgga tattgatgaa ttggaaattc actcttatga ccgtattggt cttgtgggtg
      181 ataacggagc aggaaagagt agtttactca aagtacttaa tggcgaaatt gttttagccg
      241 aagcgacatt acagcgtttt ggtgattttg cacatatcag ccaactgggc ggaatcgaaa
      301 tagaaacggt cgaagaccgg gcaatgttat ctcgccttgg tgtttccaat gtacaaaacg
      361 acacaatgag tggcggagag gaaactcgtg caaaaattgc tgccgcattt tcccaacaag
      421 tacatggcat tctagcggat gaaccaacca gccaccttga tctcaatgga atagatctac
      481 ttattggtca acttaaagca tttgatggag cattacttgt tatcagtcat gaccgatatt
      541 ttcttgatat ggttgtagac aagatatggg agttaaaaga cggtaaaatt acggaatatt
      601 ggggtggtta ctcggattac ttgcgtcaaa aagaagaaga gcgacaacac caagccgtag
      661 aatatgagct gatgatgaag gaacgggagc gattagaatc tgctgtgcaa gaaaaacgcc
      721 agcaagctaa tcgattagac aataagaaaa aaggagaaaa atccaaaaac tctaccgaaa
      781 gtgctggacg acttgggcat gcaaaaatga ctggcaccaa gcaaagaaaa ctgtatcagg
      841 cagctaagag tatggaaaag cgtttggctg cattagaaga tattcaagca ccagagcatt
      901 tgcgttctat tcgttttcgt caaagttcag ccctagaact gcacaataag ttcccgatta
      961 cggcagatgg tctgagctta aaatttggta gccgtactat ctttgatgac gctaacttta
     1021 taataccgct tggcgctaaa gtcgctataa ctggatcgaa tggaacaggg aaaacgtcct
     1081 tgttaaaaat gatatcagaa cgtgctgatg gattaaccat atctccaaaa gctgaaattg
     1141 gctactttac acaaacagga tataaattta acacgcataa atctgtgctc tcctttatgc
     1201 aggaagagtg cgagtacaca gttgcggaaa ttcgtgcagt attggcttca atggggatcg
     1261 gagcgaatga tattcaaaaa aacttatccg acttatcggg aggtgaaatc atcaaactgc
     1321 ttttatccaa aatgctttta ggaaaatata atattttgct tatggatgaa ccaggaaact
     1381 atcttgacct aaaaagtatt gccgcattag aaacaatgat gaagtcctat gcaggaacta
     1441 ttatcttcgt atctcatgac aagcaattgg tcgataatat tgctgacatt atctacgaga
     1501 tcaaagacca caaaatcatc aagacttttg agagagattg ttaatgatag ccaatctaat
     1561 ccgaacatta attattgaac tctttaaagg aaattaaaaa tgacaattca agatattcaa
     1621 tcacttgctg aagcacacgg cttgttgatt acggacaaaa tgaatttcaa tgaaatgggc
     1681 attgatttta aggtcgtttt tgctcttgat acaaaggggc aacaatggtt gctgcgtatt
     1741 cctcgtcgtg atggcatgag ggaacaaatc aagaaagaaa aacgcatttt agaattggta
     1801 aaaaaacatc tttctgtaga ggttcctgat tggagaattt catctacaga attagtggct
     1861 tatcccatac ttaaagataa tcctgtttta aatttggatg ctgaaaccta tgaaataatt
     1921 tggaatatgg acaaagatag cccgaaatac ataacatctt tggcaaaaac cttatttgaa
     1981 atccatagta ttcctgaaaa agaagttcgg gaaaatgatt tgaaaattat gaaaccttca
     2041 gatttaagac ctgaaatagc aaacaatttg cagttagtaa aatctgaaat tggtataagt
     2101 gagcaattgg aaacccgcta cagaaaatgg ttggataatg atgttctatg ggcagatttc
     2161 acccaattta tacatggcga tttatatgct gggcatgtac tagcttcaaa ggatggagct
     2221 gtttcaggcg ttattgattg gtcaacagcc catatagatg acccagcgat tgattttgct
     2281 gggcatgtaa ctttgtttgg agaagaaagc ctcaaaactc taatcatcga gtatgaaaaa
     2341 ctagggggta aagtttggaa taaactatat gaacagactt tagaaagagc agcggcctct
     2401 cctttgatgt atggtttatt tgccttagaa actcaaaatg aaagccttat cgttggagca
     2461 aaagctcagt tgggagttat ataatttaaa aatatgattg ctgaggcact gttgcaaata
     2521 gtcggtggtg ataaacttat catc
//
LOCUS       DANLXS010000382         2447 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749875-rid16719743.guided.265, whole genome shotgun sequence.
ACCESSION   DANLXS010000382 DANLXS010000000
VERSION     DANLXS010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749875
            Sequence Read Archive: SRR17934094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574578.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574578.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:37:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,723
            Genes (coding)                    :: 2,647
            CDSs (with protein)               :: 2,647
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 40 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 1 of 76
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749875-rid16719743.guided.265"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-12-09"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            94..627
                     /gene="aadB"
                     /locus_tag="PXD71_002776"
     CDS             94..627
                     /gene="aadB"
                     /locus_tag="PXD71_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="HDL6292819.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
     gene            685..1476
                     /locus_tag="PXD71_002777"
     CDS             685..1476
                     /locus_tag="PXD71_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA25"
                     /protein_id="HDL6292820.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1553..2380
                     /locus_tag="PXD71_002778"
     CDS             1553..2380
                     /locus_tag="PXD71_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034033256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-2"
                     /protein_id="HDL6292821.1"
                     /translation="MAIRIFAILFSIFSLATFAHAQEGTLERSDWRKFFSEFQAKGTI
                     VVADERQADRAMLVFDPVRSKKRYSPASTFKIPHTLFALDAGAVRDEFQIFRWDGVNR
                     GFAGHNQDQDLRSAMRNSTVWVYELFAKEIGDDKARRYLKKIDYGNADPSTSNGDYWI
                     EGSLAISAQEQIAFLRKLYRNELPFRVEHQRLVKDLMIVEAGRNWILRAKTGWEGRMG
                     WWVGWVEWPTGSVFFALNIDTPNRMDDLFKREAIVRAILRSIEALPPNPAVNSDAAR"
BASE COUNT          593 a          585 c          700 g          569 t
ORIGIN      
        1 aataaatcaa ttattattat actgctgatc accgtattag cagcaacgat gttacgcagc
       61 agggcagtcg ccctaaaaca aaggttaggc cgcatggaca caacgcaggt cacattgata
      121 cacaaaattc tagctgcggc agatgagcga aatctgccgc tctggatcgg tgggggctgg
      181 gcgatcgatg cacggctagg gcgtgtaaca cgcaagcacg atgatattga tctgacgttt
      241 cccggcgaga ggcgcggcga gctcgaggca atagttgaaa tgctcggcgg gcgcgtcatg
      301 gaggagttgg actatggatt cttagcggag atcggggatg agttacttga ctgcgaacct
      361 gcttggtggg cagacgaagc gtatgaaatc gcggaggctc cgcagggctc gtgcccagag
      421 gcggctgagg gcgtcatcgc cgggcggcca gtccgttgta acagctggga ggcgatcatc
      481 tgggattact tttactatgc cgatgaagta ccaccagtgg actggcctac aaagcacata
      541 gagtcctaca ggctcgcatg cacctcactc ggggcggaaa aggttgaggt cttgcgtgcc
      601 gctttcaggt cgcgatatgc ggcctaacaa ttcgtccaag ccgacgccgc ttcgcggcgc
      661 ggcttaactc aggtgttaga catcatgagg gaagcggtga ccatcgaaat ttcgaaccaa
      721 ctatcagagg tgctaagcgt cattgagcgc catctggaat caacgttgct ggccgtgcat
      781 ttgtacggct ccgcagtgga tggcggcctg aagccataca gcgatattga tttgttggtt
      841 actgtggccg taaagcttga tgaaacgacg cggcgagcat tgctcaatga tcttatggag
      901 gcttcggctt tccctggcga gagcgagacg ctccgcgcta tagaagtcac ccttgtcgtg
      961 catgacgaca tcatcccgtg gcgttatccg gctaagcgcg agctgcaatt tggagaatgg
     1021 cagcgcaatg acattcttgc gggtatcttc gagccagcca tgatcgacat tgatctggct
     1081 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1141 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1201 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1261 atttggtaca gcgcaataac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     1321 ataaaacgcc tacctgccca gtatcagccc gtcttacttg aagctagaca ggcttatctt
     1381 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgttcactac
     1441 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga
     1501 cgccgcttcg cggcgcggct taccttggcc gttgggcatt aaggaaaagt taatggcaat
     1561 ccgaatcttc gcgatacttt tctccatttt ttctcttgcc actttcgcgc atgcgcaaga
     1621 aggcacgcta gaacgttctg actggaggaa gtttttcagc gaatttcaag ccaaaggcac
     1681 gatagttgtg gcagacgaac gccaagcgga tcgtgccatg ttggtttttg atcctgtgcg
     1741 atcgaagaaa cgctactcgc ctgcatcgac attcaagata cctcatacac tttttgcact
     1801 tgatgcaggc gctgttcgtg atgagttcca gatttttcga tgggacggcg ttaacagggg
     1861 ctttgcaggc cacaatcaag accaagattt gcgatcagca atgcggaatt ctactgtttg
     1921 ggtgtatgag ctatttgcaa aggaaattgg tgatgacaaa gctcggcgct atttgaagaa
     1981 aatcgactat ggcaacgccg atccttcgac aagtaatggc gattactgga tagaaggcag
     2041 ccttgcaatc tcggcgcagg agcaaattgc atttctcagg aagctctatc gtaacgagct
     2101 gccctttcgg gtagaacatc agcgcttggt caaggatctc atgattgtgg aagccggtcg
     2161 caactggata ctgcgtgcaa agacgggctg ggaaggccgt atgggttggt gggtaggatg
     2221 ggttgagtgg ccgactggct ccgtattctt cgcactgaat attgatacgc caaacagaat
     2281 ggatgatctt ttcaagaggg aggcaatcgt gcgggcaatc cttcgctcta ttgaagcgtt
     2341 accgcccaac ccggcagtca actcggacgc tgcgcgataa aaccgcgcag cgccggttac
     2401 ttcaacgggc actgttgcaa atagtcggtg gtgataaact tatcatc
//
